LOCUS       DASUNT010000001         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_175402, whole genome shotgun sequence.
ACCESSION   DASUNT010000001 DASUNT010000000
VERSION     DASUNT010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175402"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /gene="aceF"
                     /locus_tag="VFX94_00005"
     CDS             <1..1175
                     /gene="aceF"
                     /locus_tag="VFX94_00005"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEX5766591.1"
                     /translation="ESDKATMEIPSPAAGMVKELIVKVGDKVSKGSAILVLESGESAA
                     RAPEKAKPAVAPAPAAPAPAQLHGTRPGPVPREPREETLAKPHASPSVRKFARELGVD
                     LALVQGSGPKGRILHSDVQAFVKGVIQQKAVPAAAKAEGGALPFNLPAWPEVDFAKFG
                     PIEAKPLSRIQKLSGPYLHRNWISIPHVTQFDEADITDLEAFRKAQTVETEKKGFKLT
                     MLAFMIKACVTALKQHPQFNASLDKSGESLIVKKYYHIGVAVDTPGGLVVPVVRDADR
                     KGVFDIAQELGDISKLARDGKLKPGDMQGGTFSISSLGGIGGTAFTPIINAPEVAILG
                     VSRSANRPVWDGKAFAPRLMLPLSLSYDHRVIDGASAARFAAFLVSVLSDIRKLIL"
     gene            1176..>2491
                     /locus_tag="VFX94_00010"
     CDS             1176..>2491
                     /locus_tag="VFX94_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5766592.1"
                     /translation="MAGTEVRVPDIGDFKDIEVIEVLVKPGDAIAKEQSLITLESDKA
                     TMEIPSPDAGVVKELRIKVGDKVSKGSPILVLEGPARGAAPAKTPEAPQAAAPTPAAG
                     ADIECDMMVLGAGPGGYSAAFRAADLGMKTVLVERYPTLGGVCLNVGCIPSKALLHTA
                     AIMDGARALAPHGISFGEPKVDLAKLRAFKDKVVGKLTGGLAGMAKARKVTVVQGVGT
                     FLDKNRLQVGGKTIRFTSAIIAAGSQAAKLPFLPEDPRIVDSTGALELKSLPKKLLVI
                     GGGIIGLEMGTVYSTLGARLDVVEMMDGLMLGADRDLVAVWQKFNAPRFDKVMLKTKT
                     TKAEAVREGIRVSFDGAEAKTYDIVLVSVGRVPNGKKIAADTAGVTVDEKGVIAVDSQ
                     MRTNAANIYAIGDIAGNPMLAHKAVHEGHVAAEAASGMKSHFDARVV"
BASE COUNT          450 a          813 c          857 g          371 t
ORIGIN      
        1 tggaatccga taaggcgacg atggagattc cctcgcctgc ggccggcatg gtgaaggaac
       61 tgatcgtcaa agtcggcgac aaggtgtcca agggttccgc gattctggtt ctggagtccg
      121 gggaaagcgc tgcaagagcg cccgaaaagg cgaagcccgc ggtggctcct gcgccggccg
      181 cgcccgctcc ggcgcagctc catggcacgc gcccgggccc ggtgccgcgc gagccgcgcg
      241 aggaaacgct cgccaagccg cacgccagcc cctcggtgcg caagttcgcg cgcgagctcg
      301 gcgtcgatct cgcgctcgtg cagggtagcg gtcccaaagg ccgcattctg cacagcgatg
      361 tgcaggcatt cgtgaaaggc gtgatccagc agaaggcggt cccggctgcg gcgaaagccg
      421 agggcggcgc gctgcccttc aacctgccgg cctggccgga agtcgacttc gccaagttcg
      481 gcccgatcga ggcgaagccc ctctcgcgca tccagaagct ctccggtccc tacctgcacc
      541 gcaactggat ctcgataccg cacgtcacgc agttcgacga ggcggacatc accgacctcg
      601 aggccttccg caaggcgcag accgtcgaga cagagaaaaa gggcttcaag ctgacgatgc
      661 tcgcgttcat gatcaaggcg tgcgtcaccg cgctcaagca gcacccgcag ttcaacgcct
      721 cgctcgacaa gtctggcgag agcctgatcg tcaagaagta ctaccacatc ggcgtggcgg
      781 tcgacacgcc gggcggactc gtggtcccgg tggtgcgcga tgccgatcgc aagggcgtgt
      841 tcgacatcgc gcaggaactg ggcgacatct cgaagctcgc gcgcgacggc aagctcaagc
      901 ccggcgacat gcagggcggc acgttctcca tttcgagcct gggcggcatc ggcggcaccg
      961 cgttcacgcc gatcatcaac gccccggaag tcgcgattct gggcgtctcg cgcagcgcga
     1021 accgaccggt atgggacggc aaggcgttcg ccccgcggct catgctgccg ctctcgcttt
     1081 cctacgacca ccgcgtcatc gacggcgcga gcgcagctcg attcgccgcg ttcctggttt
     1141 cggtgctttc cgatatccgg aagctcatcc tctagatggc cggcacagag gtaagggtcc
     1201 cggacatcgg ggacttcaag gacatcgaag tcatcgaggt gctcgtcaag ccgggcgacg
     1261 cgatcgccaa ggagcagtcg ctcatcacgc tcgagtcgga caaggcgacc atggagatcc
     1321 cgtcgccgga cgccggtgtg gtgaaggagc tgcgcatcaa ggtcggcgac aaggtgtcca
     1381 agggctcgcc gattcttgta ctggagggtc ctgcaagagg cgcagcgccg gcgaaaactc
     1441 cggaagcgcc gcaggcggcg gcgccaaccc cggccgcggg cgcggatatc gagtgcgaca
     1501 tgatggtgct cggggcgggc cctggcggct actcggcggc gttccgcgcg gccgacctcg
     1561 gcatgaaaac ggtgctggtc gagcgctatc cgacgctcgg cggggtgtgc cttaacgtcg
     1621 gctgcatccc gtcgaaggcg ctcctgcaca ccgcggcgat catggacggg gcgcgcgcgc
     1681 tcgccccgca cggcatctcc ttcggcgagc cgaaggtgga tctcgccaag ctgcgcgcgt
     1741 tcaaggacaa ggtggtcggc aagctgaccg gcgggctcgc cggcatggcc aaggcgcgca
     1801 aggtgacggt ggtgcagggc gtcggcacgt tcctcgacaa gaaccgcctg caggtgggcg
     1861 gcaagaccat ccgcttcacc tccgcgatca tcgccgccgg ctcgcaggcc gcgaagctgc
     1921 cgttcctccc ggaggatccg cgcatcgtcg actccaccgg cgcgctcgag ctgaagagcc
     1981 tgccgaagaa gctgctggtg atcggcggcg gcatcatcgg cctcgagatg ggcacggtgt
     2041 actccacgct cggcgcgcgg ctcgacgtgg tcgagatgat ggacggcctc atgctcggcg
     2101 ccgaccgcga cctcgtggcg gtgtggcaga agttcaacgc tccgcgcttc gacaaggtca
     2161 tgctcaagac caagacgacc aaggccgagg ccgtaagaga gggaatccgg gtgagcttcg
     2221 atggcgccga ggcgaagacc tacgatatcg tcctcgtctc ggtcgggcgc gtgccgaacg
     2281 gcaagaagat tgctgcggac acggccgggg tgacggtgga cgaaaaggga gtcatcgcgg
     2341 tcgacagcca gatgcgcacc aacgccgcga acatctacgc catcggcgac atcgcgggca
     2401 atccgatgct ggcgcacaag gcggtgcatg agggacacgt cgcggcggag gcggcctcgg
     2461 gcatgaagtc gcacttcgac gcgcgcgtcg t
//
LOCUS       DASUNT010000002         4017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608 k141_42,
            whole genome shotgun sequence.
ACCESSION   DASUNT010000002 DASUNT010000000
VERSION     DASUNT010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VFX94_00015"
     CDS             <1..787
                     /locus_tag="VFX94_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5766593.1"
                     /translation="LGGAANVAWNCKALGARTRLLTVVGRDEPGERLAKLLKKMAIDA
                     SLHRDAKLDTTQKLRVIGRRQQLLRIDFEKPPSREVLAAKLEEFRRALAETDVVVLSD
                     YGKGGLTHIADMIRAARKAARRVLVDPKGDDYARYKGASIITPNTAELREVVGRWTDE
                     KDLRSRAQALRAKLGLEALLMTRGEDGMTLFQKNRTISVKAEKREVMDVTGAGDTVIA
                     TLAVMLAAGAGLEAAMRIANRAAGIVVGKLGTAAATHAELFGK"
     gene            784..1761
                     /gene="rfaD"
                     /locus_tag="VFX94_00020"
     CDS             784..1761
                     /gene="rfaD"
                     /locus_tag="VFX94_00020"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEX5766594.1"
                     /translation="MKYTIVTGAAGFIGSRLVEGLNRRGVAGVVAVDNLQHSDKFRNL
                     AASEIADYVDQKDFIERLERFDGAVETVFHQGACSDTMESDGRYMMDNNYAYSKRLLE
                     WCQEEDVPFIYASSASVYGAGPQFREDRRCEKPLNVYGYSKFLFDQYVRSMLAARSAQ
                     VAGLRYFNVYGPNEQHKGRMASVAFHAYHQLRAAGKVKLFVGSGGYGNGEQRRDFVYV
                     EDVVDVNLWLLEQRDVSGVFNCGTGRAQTFNELAAAVINAVQGTALSVQEMVHKGLIE
                     YIPFPAGLAEKYQSYTQADLAQLRAAGYASDFRTVEEGVAAYVAELERK"
     gene            1758..2645
                     /gene="cysM"
                     /locus_tag="VFX94_00025"
     CDS             1758..2645
                     /gene="cysM"
                     /locus_tag="VFX94_00025"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HEX5766595.1"
                     /translation="MKKTIEDFIGNTPLVRLQRLLRSDRNILLAKLEGNNPAGSVKDR
                     PAISMIRHAERRGDIKPGDTLIEPTSGNTGIALAMAAAIRGYRMVLIMPEHLSAERRQ
                     TMRAFGAEIILTPREGGMEAARDLAERMVREKKGTMLDQFANADNPRVHYETTGPEIW
                     RDTEGRITHFVSSMGTTGTIMGCSRFFKQKDAKIQIIGCQPQEGSQIPGIRKWPEAYL
                     PKIYDRERVDRLEYVSQAEAEEMTRRLAREEGIFAGISSGGAVHVALRISKELRNAVI
                     VTIVCDRGDRYLTTGVFPD"
     gene            2688..3464
                     /locus_tag="VFX94_00030"
     CDS             2688..3464
                     /locus_tag="VFX94_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5766596.1"
                     /translation="MRPILAFDIETVPDVAGIRRLYDLPADLPDQDVAEIALQKRRAQ
                     SASDFLPPHLHRIVAIGCVLREGEGVQVFSMGEPERDEAATIQKFFDGVERYTPQLVS
                     WNGSSFDLPVLNYRMLLCGGCATKFWDQGDDDPDFRYNNYVSRYHARHCDLMDVLAMY
                     QPRNNAPLDQVAQLAGLPGKLGIGGPAVWSSYLKGEIRKIRDYCEGDCVNTYVLFLRF
                     QRQRGLLTEEQYRAETELLRSSLQATGKPYWQEFLGLWKS"
     gene            3431..>4017
                     /locus_tag="VFX94_00035"
     CDS             3431..>4017
                     /locus_tag="VFX94_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX5766597.1"
                     /translation="MAGVPRPLEELILSIDALDAEGRGVARNPQGKVVFVEGALPGER
                     VASRRIAGKRSFDVARATRVLQASGGRREPRCPHFGVCGGCATQHADERTQMAAKQRW
                     LEDNLERIGKVRPQTLLPIVYGPEWGYRRRARLSVRRVPSRGGVLVGFRERRSSYVAQ
                     MDECHVLPEAVSKLIPEMKALVEKLSVRERVPQIEV"
BASE COUNT          717 a         1337 c         1372 g          591 t
ORIGIN      
        1 cctcggcggc gcggccaacg tggcgtggaa ctgcaaggcg ctcggcgcgc gcacgcgcct
       61 gctcaccgtg gtcggccgcg acgagccggg cgagcgcctg gcgaagctcc tgaagaagat
      121 ggcgatcgac gcgagcctgc accgcgacgc caagctcgac acgacccaga agctgcgcgt
      181 catcgggcgc aggcagcagc tcctgcgcat cgacttcgag aagccgccgt cgcgcgaggt
      241 gctggcggcg aagctggagg agttccgccg cgcgctcgcc gagaccgacg tggtggtgct
      301 ctccgactac ggcaaggggg gactcacgca catcgccgac atgatccgcg cagcgcgcaa
      361 ggcggcgcgg cgcgtgctcg tcgaccccaa gggcgacgac tacgcccgct acaagggcgc
      421 gagcatcatc acgccgaaca ccgcggagct gcgcgaggtg gtggggcgct ggacggatga
      481 aaaggacctc aggagccgcg cgcaggcgct gcgcgcgaag ctcgggctgg aagcgctgct
      541 catgacgcgc ggcgaggatg gaatgacctt gttccagaag aacagaacca tcagcgtgaa
      601 ggcggaaaag cgcgaggtca tggacgtcac cggcgcgggc gacacggtga tcgccacgct
      661 cgcggtgatg ctcgccgccg gcgccgggct ggaggcggcg atgcgcatcg ccaatcgcgc
      721 cgccggcatc gtcgtcggca agctcggcac cgcggcggcc actcacgccg agctcttcgg
      781 caaatgaaat acaccatcgt caccggtgcg gccgggttca tcggctcgcg gctggtcgaa
      841 gggctgaacc ggcgcggcgt cgccggcgtg gtggcggtgg acaacctcca gcactccgac
      901 aagttccgca acctcgccgc cagcgagatc gcggactacg tcgaccagaa ggacttcatc
      961 gagcgcctcg agcgcttcga cggcgcggtg gagaccgtgt tccaccaggg cgcttgctcc
     1021 gacaccatgg agagcgacgg gcgctacatg atggacaaca actacgcgta ctcgaagcgc
     1081 ctactcgagt ggtgccagga agaggacgtg ccgttcatct acgcctcgtc ggcgtcggtt
     1141 tacggcgcgg gcccgcagtt ccgcgaagac cggcgctgcg agaagccgct caacgtctac
     1201 ggctattcga agttcctgtt cgaccagtac gtgagaagca tgctggcggc gaggagcgcg
     1261 caggtcgccg ggttgcgcta cttcaacgtc tacggcccga acgagcagca caaggggcgc
     1321 atggcctcgg tcgccttcca cgcgtaccac cagctgcgcg ccgccggcaa ggtgaagctc
     1381 ttcgtcggct ccggcgggta cggcaacggc gagcagcggc gcgatttcgt ctatgtcgaa
     1441 gatgtggtcg acgtaaacct gtggctgctc gagcagcgcg acgtgtctgg cgtgttcaac
     1501 tgcggcaccg ggcgcgcgca gaccttcaac gagctcgccg ccgcggtgat caacgcggtg
     1561 cagggcacgg cgctctcggt gcaggagatg gtgcacaagg ggctgatcga atacatcccc
     1621 ttcccggcgg ggctcgccga gaagtaccag agctacaccc aggccgacct cgcgcagttg
     1681 cgcgccgccg gctatgcgag cgatttccgc acggtggagg agggcgtcgc cgcctacgtc
     1741 gccgagctcg agcggaaatg aagaagacga tcgaagactt catcggcaac accccgctgg
     1801 tgcggctgca gcgcttgctg cgttccgacc gcaacatcct tcttgcaaaa ctggagggaa
     1861 ataatcccgc cggctcggta aaggaccgtc ccgcgatctc catgatccgg cacgccgagc
     1921 ggcgcggcga catcaagccg ggcgacacgc tgatcgagcc tacttccggg aataccggga
     1981 tcgcgctcgc aatggcggcg gccatccgcg gctaccgcat ggtgctcatc atgccggagc
     2041 atctctcggc ggagcgccgc cagacgatgc gcgcgttcgg cgccgagatc atcctcacgc
     2101 cccgcgaggg cggcatggag gcggcgcgcg acctcgccga gcgcatggtg cgcgaaaaga
     2161 agggcacgat gctcgaccag ttcgccaacg cggacaatcc gcgcgtccac tacgagacca
     2221 ccggccccga gatctggcgc gacaccgagg ggcgcatcac gcacttcgtc tcgagcatgg
     2281 gcaccaccgg caccatcatg gggtgctcgc gcttcttcaa gcagaaggac gcgaagatcc
     2341 agatcatcgg ctgccagccg caggaaggct cgcagatccc gggaatccgc aaatggcccg
     2401 aagcctacct gccgaagatc tacgaccggg agcgcgtcga ccggctcgag tatgtctcgc
     2461 aggccgaggc cgaggagatg acgcggcgcc tcgcgcgcga ggaagggatc ttcgccggca
     2521 tttcatcggg cggggcggtg cacgtggcgc tacgcatctc gaaagagctc aggaacgcgg
     2581 tgatcgtgac gatcgtgtgc gatcgcggtg atcgctatct gacgaccggg gttttccctg
     2641 actgaaaaaa gaccgggcct gaccccgatt cctgcgatcc ttgcgccgtg aggccaatcc
     2701 tcgccttcga catcgaaacc gtgcccgacg tggcgggcat ccgccggctc tacgatttgc
     2761 cggccgatct gccggatcag gacgtggcgg aaattgcgct gcaaaagcgc cgcgcgcaga
     2821 gcgcgagcga ttttctgccg ccgcacctgc accgcatcgt cgccatcggg tgcgtgctgc
     2881 gcgagggcga gggcgtgcag gttttctcca tgggcgagcc cgagcgcgat gaagccgcga
     2941 cgatccagaa gttcttcgac ggcgtcgagc gctacacccc ccagctcgtg tcctggaacg
     3001 gctcgagctt cgacctgccg gtgctcaact accgcatgct cctctgcggc ggctgcgcga
     3061 cgaagttctg ggaccagggc gacgacgacc cggacttcag gtacaacaat tacgtgagcc
     3121 gctaccacgc ccggcactgc gacctgatgg acgtgcttgc catgtaccag ccgcgcaata
     3181 acgcgccgct ggaccaggtt gcgcagctcg ccggcctgcc gggcaagctc ggcatcgggg
     3241 gccccgcggt gtggtcctcg tacctcaagg gcgagatccg caagatccgc gactactgcg
     3301 agggcgactg cgtcaacacc tacgttcttt ttctgcgctt ccagcgccag cgcgggctgc
     3361 tcaccgaaga gcaataccgg gccgagactg agctcttgcg ctcctcgctg caggcgacag
     3421 gcaagcccta ttggcaggag ttcctcggcc tctggaagag ctgattctca gcatcgacgc
     3481 gctcgacgcc gaagggcgcg gcgtcgcgcg caacccgcaa ggcaaggtcg tgttcgtgga
     3541 aggcgcgctt cccggcgagc gcgtcgcttc ccgcaggatt gcgggaaaac gcagcttcga
     3601 cgtcgcccgc gccacacggg tgctccaagc ttcgggcgga cggcgcgagc cgcgctgccc
     3661 gcacttcggc gtgtgcggcg gctgcgccac gcagcatgcc gacgagcgca cgcagatggc
     3721 cgccaagcag cgctggctcg aggacaacct ggagcgcatc ggcaaagtgc ggccgcaaac
     3781 cctgctcccg atcgtgtacg ggcccgagtg gggctatcgg cgccgcgcgc ggctctccgt
     3841 gcgacgcgtg ccctcgcgcg gcggcgtgct cgtcggcttc cgcgagcggc gctcgagcta
     3901 cgtcgcgcag atggacgagt gccacgtact gccggaggcc gtgtcaaagc tcattcccga
     3961 gatgaaggcg ctcgtggaga agctctcggt gcgcgagcgc gtgccgcaga tcgaggt
//
LOCUS       DASUNT010000003         3132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1140027, whole genome shotgun sequence.
ACCESSION   DASUNT010000003 DASUNT010000000
VERSION     DASUNT010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140027"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..649)
                     /locus_tag="VFX94_00040"
     CDS             complement(68..649)
                     /locus_tag="VFX94_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX5766598.1"
                     /translation="MKAPIDFYFDFSSPYGYLASHKIEPLAAKYGRGVTWRPILLGVA
                     FKATGAVPLPNIPLKGDYAKRDFLRSARFHGIAFRMPEPFPIPTVAPCRAFYSLKDAQ
                     AQVELAKALLRAYFVENINIGEADNVLKVAAALGCKPDLNDQAVKDRTRAEVEAALTR
                     GVFGSPYIVVDGEPFWGLDRFDQIERWLSRGPF"
     gene            complement(646..1767)
                     /locus_tag="VFX94_00045"
     CDS             complement(646..1767)
                     /locus_tag="VFX94_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5766599.1"
                     /translation="MSAQAEEIRQAVRRFAAEQLAPNAARWDREKIFPRQALQGLAAM
                     GLYGVAIAEEWGGAGLDYTSLAVACEEIAAGDCSTATIVAVNNLVAGILAGYGNRQQK
                     ERFLKPLAQGKLLGAFALTEPQVGSDASAITTRAEKQGSHYLLNGVKQFITSGQNADV
                     AVIFAVTRKQAAKRGISAFLVPTSAPGYKVARIEEKTGQRASDTAQIVLENCKAEELL
                     GEEGAGYRIALANLESGRINVAAQATGVARAALEAAIAYARERKSMGKALIEHQAVNF
                     RLADMATQVEAARQLYLHAARLRDAGKPCLKEASMAKLFASEMAEKVCSDAIQIHGGY
                     GYVSDFPVERLWRDVRVTKIYEGANDIQRLVIGRALEEG"
     gene            complement(1751..2146)
                     /locus_tag="VFX94_00050"
     CDS             complement(1751..2146)
                     /locus_tag="VFX94_00050"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5766600.1"
                     /translation="MKKPLQKLLDESRFLRPYGFKVLSVNDERCTLELPHKPQLERPG
                     GIINGPALMAAADCAMWLAIKARIGVDKDALTSELNTAFLAPAKGEHVYCTARILKMG
                     RRRIYGVAECHGKAGNLFSHHTITYVRPG"
     gene            complement(2182..2616)
                     /locus_tag="VFX94_00055"
     CDS             complement(2182..2616)
                     /locus_tag="VFX94_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX5766601.1"
                     /translation="MSELLETYRGTVYPWHCDHMGHMNVMWYVGKFDEATWNLFAAMG
                     VTTQFLKDNQRGMAAVQQNITYRRELLAGDTVFVRSAFIEVRDKAAKFVHEMCNAQTG
                     EIAAICVLTGVFIDACTRKSCPFPDDLAARGQRLVRAYALPA"
     gene            complement(2613..>3132)
                     /locus_tag="VFX94_00060"
     CDS             complement(2613..>3132)
                     /locus_tag="VFX94_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5766602.1"
                     /translation="ELVFRDCEVPASNILGAEGAGVKVLMSGLDYERAVLAGGPLGVM
                     AACLDVVIPYVHDRKQFGQPIGEFQLMQGKLADMYTAFSACRAYVYAVGQALDRNETT
                     RKDAAGAILYAAEKATWMAGQAIQALGGMGYINETPTGRLWRDAKLYEIGAGTSEIRR
                     WLIGRELFAETR"
BASE COUNT          498 a         1017 c         1046 g          571 t
ORIGIN      
        1 gatctcgttg acgaggaagc gaacgtgcga catcgacatg cgagcgcccc taggagttat
       61 tgacttacta gaatggaccg cgcgaaagcc agcgctcgat ctggtcgaac cggtcgagcc
      121 cccagaaggg ctcgccgtcg acgacgatgt acggcgagcc gaacacgccc ctggtgagcg
      181 cggcctcgac ctcggcgcgg gttctgtcct tcaccgcctg gtcgttgagg tcgggcttgc
      241 agccgagcgc tgcagccacc ttcagcacgt tgtcggcttc gccgatattg atgttttcga
      301 cgaagtacgc ccgcaacagg gctttcgcga gctccacctg cgcctgcgcg tctttcagcg
      361 aatagaaagc cctgcacggg gcaacggtgg ggatggggaa cggctcgggc atgcggaacg
      421 caatgccgtg gaagcgcgcc gagcgcagga agtcgcgctt cgcgtagtcg cccttcagcg
      481 ggatgttcgg cagcggcacc gcccccgtcg ccttgaaggc gactccaagg aggatcggcc
      541 gccacgtcac gccgcggccg tatttcgccg cgagcggctc gatcttgtgc gaggcgaggt
      601 agccgtacgg cgaggagaag tcgaaataga agtcgatcgg cgctttcatc cttcctccag
      661 ggctctgccg atcacgaggc gctggatgtc gttcgcgcct tcgtagatct tcgtcacgcg
      721 cacatcgcgc cacagccgct cgaccgggaa atcggagaca tagccgtaac cgccgtggat
      781 ctggatcgcg tcggagcaca ctttctccgc catttcggag gcgaagagct tcgccatgga
      841 cgcttccttg aggcagggct ttcctgcatc gcgcaggcgc gctgcgtgca ggtagagctg
      901 gcgcgccgct tccacctggg tcgccatgtc ggcaaggcgg aagttgaccg cctggtgctc
      961 gatcagcgcc ttgcccatgc tcttgcgctc gcgcgcgtag gcgatcgccg cctccagcgc
     1021 ggcgcgcgcg acaccggtcg cctgcgcggc gacgttgatg cggcctgact cgagattggc
     1081 gagcgcgatg cggtagccgg cgccttcctc gcccagcagc tcctcggcct tgcagttctc
     1141 gagcacgatc tgcgccgtgt cggaggcgcg ctgcccggtc ttttcctcga tgcgcgcgac
     1201 cttgtagccg ggtgccgagg tcggcacgag aaaggcgctg atcccgcgtt tggcggcctg
     1261 cttccgggtg acggcaaaga tcaccgccac atcggcgttc tggcccgagg taatgaattg
     1321 cttcaccccg ttcagcaagt aatgcgagcc ctgcttttcg gcgcgcgtgg tgattgccga
     1381 ggcatcggag cccacctgcg gctcggtcag cgcgaacgcg cccaggagct tgccttgggc
     1441 gaggggcttg aggaatcgct ccttctgttg cctgttgccg tatccggcga ggatgccggc
     1501 gacgaggtta ttcaccgcga cgatggtcgc ggtagaacag tccccggctg cgatctcctc
     1561 gcaggccacg gcgagagagg tgtagtcgag cccggcgcca ccccattcct cggcgatcgc
     1621 gaccccgtag agccccatcg cggcgaggcc ctgcagggcc tgcctgggaa agatcttttc
     1681 gcggtcccag cgcgcggcgt tcggcgcgag ctgctcggcg gcgaagcgcc gcaccgcctg
     1741 gcggatttct tcagcctggg cggacataag tgatggtgtg gtgactgaag agattgccag
     1801 ccttgccatg gcattccgcg acgccgtaga tcctgcgccg gcccatcttg aggatacgcg
     1861 cggtgcagta cacgtgctcg cctttcgcgg gagcaaggaa agcggtgttg agctccgagg
     1921 tcagtgcgtc cttgtccacc ccgatgcggg ccttgatcgc gagccacatc gcgcaatcgg
     1981 ccgccgccat cagcgccggg ccgttgatga tgccgccggg ccgctcgagc tgcggcttat
     2041 gcggcaactc gagcgtgcac ctctcgtcgt tgacgctgag caccttgaac ccgtagggcc
     2101 gcaggaagcg cgattcgtcg agcagctttt gcaggggctt tttcaagcga gctcggccgg
     2161 gtctttgcgc atggcgacgc ctcaggcggg gagcgcgtac gcgcgcacca gacgctgtcc
     2221 gcgcgcggcg aggtcgtcgg gaaacgggca cgacttgcgc gtgcaggcat cgatgaacac
     2281 gccggtcagc acgcagatcg ccgcgatctc cccggtctgc gcgttgcaca tctcatgcac
     2341 gaatttcgcc gccttatcgc gcacttctat gaatgccgag cgcacgaaga cggtatcgcc
     2401 ggcaagcagc tcgcgccggt aggtgatgtt ctgctggacc gcggccatgc cgcgctgatt
     2461 gtccttcagg aactgcgtgg tcacgcccat ggctgcgaac aggttccacg tcgcctcgtc
     2521 gaacttgccg acgtaccaca tgacgttcat gtggcccatg tggtcgcaat gccagggata
     2581 caccgtgccg cgataggtct cgaggagctc gctcatcggg tttcggcgaa gagctcgcgg
     2641 ccgatcagcc agcggcggat ctcggaggtt ccggcgccga tctcgtagag cttggcgtcg
     2701 cgccaaagcc tcccggtggg cgtctcgttg atgtagccca tgccgccgag cgcctggatc
     2761 gcctggcccg ccatccaggt cgctttttcg gcggcgtaga ggatcgcgcc ggccgcgtcc
     2821 ttgcgggtcg tttcgttccg gtccagcgcc tggcctacgg cgtagacgta ggcgcggcag
     2881 gcggagaacg cggtgtacat atcggcgagc ttgccctgca tgagctggaa ctcgccgatc
     2941 ggctggccga actgcttgcg atcgtgcacg tacgggatca cgacatcgag gcaggcggcc
     3001 atgacgccga gcggcccgcc ggcgagcacc gcgcgctcgt agtcgaggcc ggacatgaga
     3061 actttcaccc ccgcaccttc ggcgcccaga atgttgctgg caggaacttc gcagtcgcgg
     3121 aacaccagct cg
//
LOCUS       DASUNT010000004         2353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_702105, whole genome shotgun sequence.
ACCESSION   DASUNT010000004 DASUNT010000000
VERSION     DASUNT010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702105"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFX94_00065"
     CDS             complement(<1..191)
                     /locus_tag="VFX94_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766603.1"
                     /translation="MRALIWLLMGIPLLVQAQPLPIFDAHIHYSHDAWDVVPVQDAIA
                     IMRKAGVKRALVSSSGDDG"
     gene            219..764
                     /locus_tag="VFX94_00070"
     CDS             219..764
                     /locus_tag="VFX94_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX5766604.1"
                     /translation="MQVSPPRSLGELFLGFLSIGARSFGGVMPWAHRVMVEERRWMTE
                     ADFTETIGLCQFLPGPNIGNASIVVGKRWFGLRGALVAFLGLFALPFVWVLALFALYA
                     DFAATNATLRAVVTGVGAAGAGLFIGTAIKLGRVLVRKPVALALIVTCFVAAGILRVS
                     LFLVLPLSMAVSYLCARKGWL"
     gene            761..1279
                     /locus_tag="VFX94_00075"
     CDS             761..1279
                     /locus_tag="VFX94_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX5766605.1"
                     /translation="MTVLELALYFMLLSLISVGGMPSVLPEMQRYVVDVKGWMTPQDF
                     MQIFAVGQAAPGPNILIASLVGWKVAGLTGALVALAAMCGPAAVLAWWVAEAWERFKD
                     EPWRIAAQKAISPIVVALILSGGFILATPGAPDWRLWLIAAATAVGMLVSKINPLWFL
                     AAGGALGAFLLE"
     gene            complement(1282..1566)
                     /locus_tag="VFX94_00080"
     CDS             complement(1282..1566)
                     /locus_tag="VFX94_00080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013954173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX5766606.1"
                     /translation="MSFVLVVNIRIKPENVNAFMKQLNENAAAARKEPGCKQFDVLVD
                     PKDKAKVMLYEVYNDEKAFEAHQQTPHFKKYVAEAVPMLAARERSVWQRT"
     gene            1619..2065
                     /locus_tag="VFX94_00085"
     CDS             1619..2065
                     /locus_tag="VFX94_00085"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5766607.1"
                     /translation="MQARAPKLLFPQLQSFYQWAEPLSWALIRITAGLMLIPHGWPKL
                     MMGVGATAQMALVKRGIYPAEPLAVILIAIETLGGLCIALGLFTRFWAAAATIEMAVI
                     VYHHLPKFGWTDRGYEYPLFWGLVMFAIALRGGGPYSVDRVIGREL"
     gene            2077..2153
                     /locus_tag="VFX94_00090"
     tRNA            2077..2153
                     /locus_tag="VFX94_00090"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2111..2113,aa:Arg,seq:acg)
BASE COUNT          355 a          796 c          713 g          489 t
ORIGIN      
        1 tgcccgtcgt cgcccgagct cgagacgagc gcgcgcttca cgccggcctt gcgcatgatc
       61 gcgatcgcgt cctgcaccgg caccacgtcc caggcgtcgt ggctgtaatg gatgtgtgcg
      121 tcaaagatcg gcagcggctg agcctgcacg agaagcggga ttcccatcaa gagccagatt
      181 agggcgcgca tcggcccaag tgtatattcc cggaaaacat gcaggtaagt cccccgcgct
      241 cgctcggcga actcttcctc ggctttctct ccatcggcgc gcgctccttc ggcggcgtga
      301 tgccgtgggc gcaccgcgtc atggtggagg agcggcgctg gatgaccgaa gcggacttca
      361 ccgaaaccat cggcctttgc cagttcctgc ccgggccgaa catcggcaat gcatcgatcg
      421 tcgtcggcaa gcgctggttc gggttgcgcg gcgcgctcgt cgcgttcctc ggtctgttcg
      481 cgctgccctt cgtctgggtg ctcgccctct tcgcgctcta cgccgatttc gccgccacga
      541 acgcgaccct gcgcgccgtc gtgaccgggg tcggcgccgc gggcgcgggg cttttcatcg
      601 gcacggcgat caagctgggg cgcgtgctgg tgcgcaagcc cgtggcgctc gcgctcatcg
      661 tcacgtgctt cgttgccgcc ggcatcctgc gagtgtcgct ctttctcgtc ctgccgctgt
      721 cgatggcggt ttcatacctc tgcgcacgca agggctggct atgacggtgc tcgagctcgc
      781 gctctacttc atgctccttt cgctcatctc ggtgggcggc atgccctcgg tgctgcccga
      841 gatgcagcgc tacgtggtgg atgtcaaggg atggatgacg ccgcaggact tcatgcagat
      901 cttcgccgtc ggccaggcgg cgccagggcc caacatcctg attgcgagcc tcgtcggctg
      961 gaaggtcgcc gggctcacgg gagcgctcgt tgcgctcgct gccatgtgcg gcccggccgc
     1021 cgtgctcgcc tggtgggtgg ccgaagcctg ggagcgcttc aaggacgagc cctggcgcat
     1081 cgccgcccag aaagccatct cgccgatcgt cgtcgcgctc atcctctccg gcggcttcat
     1141 cctcgccacg cccggcgcgc ccgactggcg cctgtggctc atcgccgccg cgactgccgt
     1201 cggaatgctc gtctcgaaga tcaacccgct gtggtttctc gccgcgggcg gcgctctcgg
     1261 cgcttttcta ctcgaataac tctacgtccg ctgccacacc gacctttccc tggcagcaag
     1321 catcgggacc gcttcggcca cgtacttctt gaagtgcggc gtctgctggt gcgcctcgaa
     1381 cgccttctcg tcgttgtaga cctcgtacaa catcaccttc gccttgtcct tcggatccac
     1441 cagcacgtcg aactgcttgc agcccggctc cttgcgcgcg gcggccgcgt tctcgttcag
     1501 ctgcttcatg aacgcattca cgttctcggg cttgatgcgg atgttcacca ccagtacgaa
     1561 gctcatcgtc tctcctcgcc tcctttagag taatctactc gaagagggag gaaaaaacat
     1621 gcaggcaaga gcgccgaagc tcctgttccc gcagctccag tcgttctacc agtgggccga
     1681 gccgctgtcg tgggccctca tccgcattac cgccggcctg atgctcatcc cgcacggctg
     1741 gccgaagctc atgatgggcg tcggcgccac cgcgcagatg gcgctcgtca agcgcggcat
     1801 ctatcccgcc gagccgctcg ccgtcattct gatcgccatc gaaacgctag gcggactttg
     1861 catcgcgctc ggtctcttca cgcgcttttg ggccgcggcc gcgaccatcg agatggcggt
     1921 catcgtttat caccacctgc cgaaattcgg ctggaccgat cgcggctacg aatacccgct
     1981 tttctggggc ctcgtgatgt tcgccatcgc gctgcgcggc ggcggcccat actcggtcga
     2041 ccgcgtgatc ggccgcgagt tataattcgc ggcctcgcgc ccgtagctca gttggataga
     2101 gtacctggct acgaaccagg gggtcgtggg ttcaaatcct gccgggcgcg ccaggaaaat
     2161 caaggtgcat tccggtcaca taggtaacgt tttataccgg agacatgggt aacacttttg
     2221 cggcgaacgg attatccacc ggttcgacgc gacatgtctc gtgatcgaaa tagcccaagt
     2281 cgtagttcat gaaggtgacg agccagatct tgtcgctcac ctccttgacc ccgacgtctt
     2341 ggccagcaaa gac
//
LOCUS       DASUNT010000005         4831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_526754, whole genome shotgun sequence.
ACCESSION   DASUNT010000005 DASUNT010000000
VERSION     DASUNT010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526754"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VFX94_00095"
     CDS             complement(<1..796)
                     /locus_tag="VFX94_00095"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX5766608.1"
                     /translation="MTDAVIVSTARTPLCKSWRGALNLTHGAKMGGHVAHAALERAKV
                     DGAEVEDVIIGCANPEGATGWNIARQIAIRAGCPITVPGMTVNRFCSSGLQAIALAAQ
                     RILAGEGEVFVAGGIESISLVQNEMNKHHFQDEWLHRHKPETYWPMLQTAEYVARRYE
                     IPREKQDEFGVQSQQRAARARAEGKFKDEIVPITTKMKVVDKNTGQESVKEVTATEDE
                     GIRPDTTYEGVSQIKPAIEGGCIAAGNASQFSDGASAAVVMSAKEAA"
     gene            complement(845..2932)
                     /locus_tag="VFX94_00100"
     CDS             complement(845..2932)
                     /locus_tag="VFX94_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX5766609.1"
                     /translation="MSDVAHYAVRDAIAVVTMNNPPVNGLGNALRAALMAHLKNAEAD
                     RAVRAVLIMGSAKAFSGGADIREFNKPREKPDLFEVNDQQDAMSKPLVAAIGGFALGG
                     GLELALGCHYRVALPRAQLGLPEVKLGILPGSGGTQRLPRVIPMAEAVRMMTTGATIP
                     AEKAKELGLVDEIVSGDLLEGSLQFTRKLLAAGRGPRRVRDMKAKLDGDAHEFFAKVR
                     EQVANESRGYPAPLEIVACAEAAATLPFDEGRKVERERFAHLVNTPESKALRHMFFAE
                     RQTTKIPDVPEDTPARPIGKAAVIGAGTMGGGIAMCFANAGIPVTVVETTQQALDKGL
                     ERIKANYAATVAKGRLAQTDMDKRMGLIRGTTNLAQAGDADIVIEAVFERMDVKQDLL
                     RKLDAIAQKGAILATNTSTLDVDKIAAATTRPEDVIGTHFFSPANVMRLLEVVRGKKT
                     AKDVLATTIRLAKQLKKLPVVSGVCDGFIGNRMLEKYTQQSLFLLEEGATPAQVDGAL
                     QRWGMAMGPFTMYDMAGNDIGWEIRKRRAKERPDMVYSRFADRICEKGWFGQKAGRGW
                     YRYEKGSRKPIPDEEIARMLSDYRREIGVTPRKISDEELVERCIYALANEGARILEEG
                     IALRASDIDMVYLTGYGFPPYRGGPMFYADQVGLDKVLASIRRFQAGYQGSQWQVAPL
                     LEELAKDGKRFNQ"
     gene            complement(2977..3903)
                     /gene="bioB"
                     /locus_tag="VFX94_00105"
     CDS             complement(2977..3903)
                     /gene="bioB"
                     /locus_tag="VFX94_00105"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX5766610.1"
                     /translation="MDVKDVLGLFRLPFTELVFRAAAVHRAHFDPSQIQLSSLLSVKT
                     GGCPEDCAYCPQSRRYATGVRDEDMLPLEQVVESARVAKAKGASRFCMGAAWRTPKDR
                     DLEKIEQFIRAVKQLGLETCVTLGMLKPGQAERLRDAGLDYYNHNLDTSPQFYGEIIS
                     TRTYQDRLDTLERVRAAGIRVCCGGIVGMGESREDRAALIAQLSSLDPHPESVPINSL
                     IRVKGTPLHGADPVDPLEFVRTIAAARICMPKAWVRLSAGRETMPEALQALCFLAGAN
                     SVFYGEKLLTTPNPEIDTDRALFERLGLTPTA"
     gene            3939..>4831
                     /locus_tag="VFX94_00110"
     CDS             3939..>4831
                     /locus_tag="VFX94_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5766611.1"
                     /translation="MHGLMMDQQLLISDLIRHADRHHGSTDIVSKTVEGGIHRYTYRE
                     AHARIRRLANALKRLGVGMHDRVATLAWNGFRHFEVYYAVAGSGAVIHTINPRLFPDQ
                     VGYIANHAEDKVVFYDTTFAPLIEKLRPQLKTVKRFIALTDEYEELISSEADTFDWPS
                     FDERTAACLCYTSGTTGNPKGALYSHRSTLIHAYAAALPDTLNLSAREVVLPVVPMFH
                     VNAWSLPYSCTMTGAKMVFPGPHLDGKSLHELFETEGVTMSAGVPTVWLGLLNHVKEK
                     KLRFSTLKNVVIGGSACLPAM"
BASE COUNT          740 a         1622 c         1563 g          906 t
ORIGIN      
        1 gcgcggcttc cttggcgctc atcacgactg ccgccgaagc gccgtcggag aactggctcg
       61 cgttgcccgc ggcgatgcat ccgccctcga tcgccggctt gatctgcgac accccttcgt
      121 acgtcgtgtc aggacggatg ccttcgtcct cggtcgctgt cacttccttg accgactcct
      181 ggccggtgtt cttgtccacc accttcatct tggtagtgat cggcacgatc tcgtccttga
      241 acttgccttc ggcgcgcgcc ctggcggcac gctgctggct ttgcacgccg aactcatcct
      301 gcttctcgcg cgggatctcg tagcggcgcg ccacgtactc ggcggtctgc agcatcggcc
      361 agtaggtctc gggcttgtgg cggtgcagcc actcgtcctg gaagtgatgc ttgttcatct
      421 cgttctgcac gagcgagatc gactcgatgc cgcccgcgac gaacacctcg ccttcgcccg
      481 cgaggattct ttgggctgcc agggcgatgg cctgcaggcc ggaggagcag aagcggttca
      541 ccgtcatgcc gggcacggta atcgggcagc cggcgcggat cgcgatctgc cgcgcgatgt
      601 tccatccggt cgcgccctcg gggttggcgc agccgatgat cacgtcctcg acctcggcgc
      661 catccacttt cgcgcgctcg agcgcggcgt gcgccacgtg gccgcccatc ttggcgccat
      721 gggtcaggtt gagcgcgccg cgccagctct tgcagagcgg cgtgcgcgcg gtggaaacga
      781 tcaccgcgtc tgtcatgtcg ggctcctcct cgcatccaag agtgttcgct tcactaaatc
      841 gtcttcattg attgaagcgc ttgccgtcct tggccagctc ctccagcagg ggcgccacct
      901 gccactggct tccctggtag cccgcctgga acctgcgtat cgaggcgaga accttgtcga
      961 gccccacctg gtcggcgtag aacatcggcc cgccgcggta cggcggaaag ccgtagcccg
     1021 tgaggtacac catgtcgatg tcggaggcgc gcagcgcgat cccctcctcg aggatgcgcg
     1081 cgccctcgtt ggcgagcgcg tagatgcagc gctcgacgag ctcttcgtcg gagatcttgc
     1141 ggggtgtgac gccgatttcc ctcctgtagt ccgaaagcat ccttgcgatt tcttcatccg
     1201 ggatcggctt gcggcttccc ttctcgtagc ggtaccagcc gcggcccgcc ttctgcccga
     1261 accagccttt ctcgcagatg cggtcggcga acctggaata caccatgtcc gggcgctcct
     1321 tcgcgcgccg cttgcggatc tcccagccga tgtcgttgcc cgccatgtcg tacatcgtga
     1381 acgggcccat cgccatgccc cagcgctgga gcgcgccgtc cacctgcgcg ggcgtcgcgc
     1441 cctcctcgag cagaaaaagg ctctgctgcg tgtatttctc gagcatgcgg ttgccgatga
     1501 agccgtcgca gaccccggac acgaccggca gcttcttcag ttgttttgcg agccttatcg
     1561 tggtggcgag gacgtctttc gcggtcttct ttccgcgcac gacctcgagc agccgcatga
     1621 cgttcgccgg cgagaagaag tgcgtcccga tcacatcctc gggccgcgtg gtggcggcag
     1681 cgatcttgtc cacgtccagt gtcgacgtat tggtggcgag gatcgcgcct ttctgcgcga
     1741 tagcgtcgag cttcctgagg aggtcttgct tcacgtccat gcgctcgaac accgcctcga
     1801 tcacgatgtc ggcatccccc gcctgggcga gattcgtcgt gccgcggatg agccccatgc
     1861 gcttgtccat gtcggtttgc gcgaggcggc ccttggcgac cgtggcggca tagttggcct
     1921 tgattctctc caggcccttg tccagggcct gctgcgtagt ctccaccacc gtgaccggga
     1981 tgccggcatt cgcgaagcac atcgcgatgc cgccccccat ggtgcccgcg ccgatgaccg
     2041 ccgccttgcc gatcggccgc gcgggcgtgt cttccggcac gtccgggatc ttggtggtct
     2101 ggcgctcggc gaagaacatg tgacgcagcg ccttcgattc cggcgtgttc acgagatggg
     2161 cgaagcgctc gcgctcgacc ttgcggccct cgtcgaacgg tagcgtcgcc gcagcttccg
     2221 cgcatgcgac gatctcgagc ggcgccgggt agccgcgcga ttcgttcgcc acctgctcgc
     2281 gcaccttggc gaagaactcg tgcgcgtcgc cgtcgagctt cgctttcatg tccctgactc
     2341 gtctcggacc gcggccggcg gcgagcaatt tcctcgtgaa ctgcaaagac ccttcgagga
     2401 gatccccgct gacgatctcg tccacgaggc cgagctcctt cgctttctcc gcggggatcg
     2461 tggcgccggt cgtcatcatg cgcaccgctt ccgccatggg gatgacgcgc ggcaggcgct
     2521 gggtgccgcc cgagccgggc aggatgccga gcttgacctc tggcaggccc aactgcgcgc
     2581 gcggcagcgc gacgcggtaa tggcagccga gcgcgagctc gagcccgccg ccgagcgcaa
     2641 acccgccgat cgcagcgacg aggggcttgc tcatcgcgtc ctgctggtcg ttcacctcga
     2701 aaaggtccgg cttttcgcgc ggcttgttga actcgcggat atccgcgccg ccggagaaag
     2761 ccttcgccga gcccatgatg agcaccgccc tgaccgcccg gtccgcctcg gcgtttttca
     2821 ggtgggccat caaagcggcg cgcagcgcat tccccagccc gttcaccggc ggattgttca
     2881 ttgtgactac cgcgatggcg tcgcggacag cgtagtgagc gacgtcgctc atgcgagagg
     2941 gactcctgaa gtcaaggggt gcgttcggtc tcggggtcaa gccgtcggtg tgaggcccag
     3001 gcgctcgaag agcgcccggt ccgtgtcgat ttccgggttg ggggtggtga gaagcttttc
     3061 gccgtagaac accgagttcg cgccggcgag gaagcatagc gcctgcagcg cctcgggcat
     3121 ggtttcgcga cccgcggaga ggcgcaccca cgccttcggc atgcaaatgc gcgccgccgc
     3181 tatggtgcgc acgaattcga gcgggtcgac gggatccgcg ccatgaagcg gcgtgccttt
     3241 gacccggatc agggaattga tggggacact ttccggatgc ggatcgaggc tggaaagctg
     3301 cgcgatgagc gcggcgcggt cttcgcgcga ttcgcccatg ccgacgatgc cgccgcagca
     3361 cacgcggatg ccggcggcgc gcacgcgctc gagggtgtcg agacggtcct ggtaggtgcg
     3421 agtgctgatg atctcgccgt agaactgcgg cgaagtgtcg aggttgtggt tgtagtagtc
     3481 gagccctgca tcgcgcagcc gctcggcctg gccgggcttc agcatgccga gggtgacgca
     3541 ggtttccagc ccgagctgct tcactgctcg tatgaattgc tctattttct cgagatcgcg
     3601 atccttcggc gtgcgccagg cggcgcccat gcagaagcgg ctcgcgccct tcgctttcgc
     3661 aactctcgcc gactcgacca cctgctcgag cggcagcatg tcctcatccc gcaccccggt
     3721 ggcgtagcgg cgcgactgcg ggcagtaggc gcagtcctcc gggcagccgc cggttttcac
     3781 cgagagcagg ctcgagagct ggatctgcga cggatcgaag tgggcgcgat gcacggcggc
     3841 ggcccggaag acgagctcgg tgaacggcag cctgaaaagg ccgagcacgt ccttgacgtc
     3901 catggcgcta ggataaacta cttcgcgagg aggaagtcat gcacggactg atgatggatc
     3961 agcagcttct catcagcgac ctgatccggc atgcggaccg ccaccacggc tcgaccgaca
     4021 tcgtgtcgaa aacggtggag ggcggcatcc accgctacac gtaccgcgag gcgcacgccc
     4081 ggatccgccg gctggccaac gcgctcaagc gcctgggcgt cggcatgcac gaccgggtgg
     4141 cgacactcgc ctggaacggc ttcaggcact tcgaggtgta ctacgcggtc gcgggctcgg
     4201 gcgcggtgat ccacaccatc aacccgcgtc tatttcccga ccaggtcggc tacatcgcga
     4261 accacgccga ggacaaggtc gttttctacg acaccacgtt cgcgccgctc atcgagaagc
     4321 tcaggccgca gctgaagacc gtcaagcgct tcatcgcgct gaccgacgaa tacgaagagc
     4381 tgatttcgag cgaagcagac acgttcgact ggccgagctt cgacgagagg accgcggcgt
     4441 gcctttgcta cacctcgggc accaccggaa acccgaaggg cgcgctctac tcgcatcgct
     4501 ccaccctcat tcatgcctac gcggccgcgc tgcccgacac gctgaacctc tcggcgcgcg
     4561 aagtggtgct gcccgtcgtg ccgatgttcc atgtcaacgc ctggagcctg ccgtactcgt
     4621 gcaccatgac cggcgcgaag atggtgttcc ccggcccgca cctcgacggc aagagcctgc
     4681 atgagctctt cgagaccgag ggcgtgacga tgagcgccgg cgtgcccacg gtgtggctgg
     4741 ggctgctcaa tcacgtgaag gagaagaagc tgcgcttctc cacgctgaag aacgtggtga
     4801 tcggcggctc ggcctgcctg ccggcgatga t
//
LOCUS       DASUNT010000006         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_88391, whole genome shotgun sequence.
ACCESSION   DASUNT010000006 DASUNT010000000
VERSION     DASUNT010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88391"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFX94_00115"
     CDS             complement(<1..264)
                     /locus_tag="VFX94_00115"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX5766612.1"
                     /translation="MSGATPGLSRRLASMVYEAVLLFAVAFFAAWLFFFASGGREALS
                     GWLRHALQLFIIAAFAAYFLWCWLRSGQTLAMKAWNIRLVDVTP"
     gene            complement(261..740)
                     /locus_tag="VFX94_00120"
     CDS             complement(261..740)
                     /locus_tag="VFX94_00120"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HEX5766613.1"
                     /translation="MMRAAWLLAATVFAGAALAEPPVEVAPKTKPAPKAKPAAKAKAQ
                     VRPAWAELTAEQQQVLAPLKPDWESLEPERRRKWIGVAKRYPQMTPEAQERVQRRMQT
                     WAKLSPEQRRQARETYKQIVKAPPEKRANLRQQWAEYQQLPQQERDSLVSPEGKRRR"
     gene            complement(737..1117)
                     /locus_tag="VFX94_00125"
     CDS             complement(737..1117)
                     /locus_tag="VFX94_00125"
                     /inference="COORDINATES: protein motif:HMM:NF023699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HEX5766614.1"
                     /translation="MNEMQFGRRIRQILNQGTEVNPRVAERLRQAREAALARQRPEPA
                     PAFAWADHALVRFGGWSGLALRVLLPLAMIVASGVAVYAWEQNRRAAELEEIDAQLLT
                     DDLPIDAYLDRGFQNWLKKRAAEQ"
     gene            complement(1114..1695)
                     /locus_tag="VFX94_00130"
     CDS             complement(1114..1695)
                     /locus_tag="VFX94_00130"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5766615.1"
                     /translation="MASRSELAAFLEGVEKRAFKQAMFAVRDEEAALDIVQDAMLRLA
                     EKYGHLPASELPALFHRIVQNAIRDWYRRQKVRSMWTTLLSSVGPGRDDDDEHDPLET
                     LEPAYGSDARASPAAQLEQRQVVEIIREEIFRLPERQREAFILRYWDELDVAETARKM
                     NCSEGSVKTHCSRAAHALQAALAARGITFETKS"
     gene            1821..>2018
                     /locus_tag="VFX94_00135"
     CDS             1821..>2018
                     /locus_tag="VFX94_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5766616.1"
                     /translation="MDRAKTTALTGAEIVVRCLQAEGVEYVFGYPGGAVLFIYDELFK
                     QDKVKHVLVRHEQGAVHAADGY"
BASE COUNT          314 a          680 c          667 g          357 t
ORIGIN      
        1 cggcgtaacg tcgacgaggc ggatgttcca ggctttcatc gcgagcgtct gcccgctgcg
       61 tagccagcac cagaggaagt aagcggcgaa cgccgcgatg atgaagagct ggagcgcgtg
      121 gcgcagccag cccgaaagcg cctcgcggcc gccgctcgcg aagaagaaga gccacgcggc
      181 gaagaaggcg accgcgaaga gcagtaccgc ctcgtacacc atgctcgcga gccggcgcga
      241 caggcccggc gtggcgccgc tcaacggcgg cgcttccctt ccggggaaac caggctgtcg
      301 cgctcctgct gcggcagctg ctggtactcg gcccactgct ggcgcaggtt ggcgcgcttc
      361 tcgggcggag ccttcacgat ctgcttgtaa gtctcgcgtg cctggcgcct ctgctcgggc
      421 gagagcttcg cccaggtctg catgcgccgc tgcacgcgct cctgggcctc cggcgtcatc
      481 tgcgggtagc gcttagcgac gccgatccac ttgcgccggc gctccggctc gaggctttcc
      541 cagtcgggct tgagcggtgc gaggacctgc tgctgctcag cggtgagctc cgcccacgcc
      601 ggccgcacct gcgccttcgc cttggccgcg ggcttcgcct ttggggccgg cttcgttttg
      661 ggggcgacct cgaccggcgg ctcggcgagc gccgcgcctg cgaagaccgt ggcagcgaga
      721 agccaagcgg cgcgcatcat tgctcggccg cgcgcttctt gagccagttc tggaagccgc
      781 gatcgaggta ggcgtcgatc ggcaggtcgt cggtgaggag ctgtgcgtcg atctcttcca
      841 gctccgccgc gcgccggttc tgctcccagg catagaccgc gaccccgctc gctacgatca
      901 tggcaagcgg cagcagcacg cgcagcgcta gcccgctcca gccgccgaag cggacgagcg
      961 catggtccgc ccacgcgaag gccggcgcgg gctccggacg ctggcgcgcg agcgcagcct
     1021 cgcgcgcctg ccgcaggcgc tcggcaaccc ttggattgac ctcggttccc tgattcagga
     1081 tttgacggat cctgcggccg aattgcattt cattcatgat ttcgtctcga acgtgatgcc
     1141 gcgggccgct agggctgctt gcaaagcatg agcggctcgg gagcagtgcg ttttcacgct
     1201 gccctccgag cagttcatct ttcgggccgt ttcggccacg tccagttcat cccaataacg
     1261 cagtatgaaa gcttcccgtt gacgctccgg caagcggaaa atctcttccc taataatttc
     1321 aacgacttga cgctgctcga gttgtgcggc cggactcgct cttgcgtctg atccgtaggc
     1381 gggttccagg gtctcgagcg ggtcgtgctc gtcgtcatcg tcccgccccg ggccgacgga
     1441 tgaaagcagc gtagtccaca tcgagcgcac cttctggcgc cggtaccagt cgcgaatcgc
     1501 gttctgcacg atgcgatgga aaagcgccgg cagctcggag gccggcaagt ggccgtactt
     1561 ctccgcgagc cgcagcatcg cgtcctgcac gatgtcgagc gccgcctcct cgtcgcgcac
     1621 ggcgaacatg gcctgcttga acgcgcgctt ctcaacgcct tcgaggaaag cggcaagctc
     1681 gcttcgggat gccaggaccg gccctctgtt gtttggcggc cgatggtatc aaaccgaccg
     1741 cgcgaggccg gggcgctcgg cttgaccgca ccgcaccaag cctctattct tcaaggcttt
     1801 tccgacactt agaagggtta atggaccggg ccaagaccac cgcgctcacc ggcgcggaaa
     1861 tcgtcgtgcg ctgcctgcag gccgagggcg tcgagtacgt gttcggctac cccggcggcg
     1921 cggtgctgtt catctacgac gagctcttca agcaggataa agtgaagcac gtcctggtgc
     1981 ggcacgagca gggtgcggtg catgccgccg acggctac
//
LOCUS       DASUNT010000007         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_614531, whole genome shotgun sequence.
ACCESSION   DASUNT010000007 DASUNT010000000
VERSION     DASUNT010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614531"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="VFX94_00140"
     CDS             <1..1210
                     /locus_tag="VFX94_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEX5766617.1"
                     /translation="DITARNPGDWAKEYGVTTKIRSDDNTRFRIQVVYHPQGASADAV
                     VESFENLSMLPGDPREAVKVLEAESSFVRAVVLGGATNPPADTPSAQMLSGAANLDGT
                     VLQPDSAPFETALQAGGGAGGVNLLDEIDLFNLLCVPGETKNTIVSELEKFCRDRRAF
                     LIVDAPQGASFTSLQNGPDSQIVGDNAINAALYFPWVLAPDPLQENRPAEYPAAPLVA
                     GIYARTDSQRGVWKAPAGTDASLSGVLGLRTPLTDLQNGELNIRAINCLRTLTVFGNV
                     VWGARTLRGNNEVGSEWKYIPVRRMALFIEETLYRGTKWVVFEPNDEPLWAQIRLNIG
                     AFMQTLFRQGAFQGRSPREAYFVKCDGETTTQTDINLGIVNIHVGFAPLKPAEFVVIR
                     IQQMAGQTEA"
     gene            1223..1750
                     /locus_tag="VFX94_00145"
     CDS             1223..1750
                     /locus_tag="VFX94_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX5766618.1"
                     /translation="MAQFSVNAQRFDPYKNFKFRVKWDGRYVAGVSKVGALKRSTEMI
                     EHREGGDPSTSRKSPGRTKYEAITLERGVTHDSEFEKWANSVWNYGSGLGAEVSLKTF
                     RKDIIIEVYNEAGQLVLAYKVYRCWVSEFQALPDLDANANAIAIQTLKLENEGWERDY
                     DVTEPAEPQFSEPPQ"
     gene            1750..>2395
                     /locus_tag="VFX94_00150"
     CDS             1750..>2395
                     /locus_tag="VFX94_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766619.1"
                     /translation="MPALDDAALLALWERGAALHPLDRALLLCGAARADVAPARLADL
                     PLGEANRALLELRRASFGPHLAALVACERCAAPLEIELHADALAAALGAPRAAAGASL
                     RALTMRDLAVVSGEPTAEAAALALARRCALAGGHDALSREELERGLEALDPAADIALD
                     MTCEACGHAWRASLDLAAFLWTEVAARAAAILGDVHRLACAYGWTEREILELGPQ"
BASE COUNT          390 a          874 c          795 g          336 t
ORIGIN      
        1 cgacatcacg gcgcgcaatc ccggcgactg ggcgaaggag tacggcgtca cgaccaagat
       61 caggtcggac gacaacacgc gctttcgcat ccaggtcgtc taccacccgc agggcgcgag
      121 cgccgacgcg gtggtcgagt cgttcgagaa cctgtcgatg ctcccgggcg acccgcgcga
      181 ggcggtgaaa gtgctggaag ccgaatcgag cttcgtccgc gccgtggtgc tcggcggcgc
      241 caccaacccg cccgcggaca ccccgtcggc gcagatgctc tcgggcgcgg ccaacctcga
      301 cggcaccgtg ctgcagcccg acagcgcccc gttcgagacc gcgctgcagg cgggcggcgg
      361 cgcaggcggc gtcaacctcc tcgacgagat cgatctcttc aacctgctct gcgtgccggg
      421 cgagacgaaa aacacgatcg tgtccgagct cgagaaattc tgccgcgacc ggcgtgcgtt
      481 cctcatcgtc gatgcgcccc agggcgcgag cttcacgagc ctgcaaaacg gacccgattc
      541 ccagatcgtg ggcgacaacg ccatcaacgc cgcgctctac ttcccgtggg tgctcgcgcc
      601 cgacccgctg caggagaacc gtcccgcgga atatccggcc gcccccctgg tcgccggcat
      661 ctacgcccgc accgactcgc agcgcggcgt ctggaaggcg cctgcgggaa ccgacgccag
      721 cctgagcggc gtgctcgggc tgcgcacgcc gctcaccgac ctgcagaacg gcgagctcaa
      781 catccgcgcc atcaactgcc tgcgcacgct cacggtattc ggcaacgtgg tgtggggcgc
      841 gcgcaccctg cgcggcaaca acgaggtcgg ctcggaatgg aagtacatcc cggtgcgccg
      901 catggcgctc ttcatcgagg agacgctcta ccggggcacg aagtgggtgg tgttcgagcc
      961 gaacgacgag ccgctctggg cccagatccg cctgaacatc ggcgccttca tgcagacgct
     1021 cttccgccag ggcgccttcc agggccgctc gccgcgcgag gcgtacttcg tgaagtgcga
     1081 cggcgagacc accacgcaga ccgacatcaa cctcggcatc gtcaacatcc acgtcggctt
     1141 cgcgccgctc aagcccgccg agttcgtggt catccgcatc cagcagatgg ccggtcagac
     1201 cgaagcgtaa ggaggcaccc cgatggccca gttcagcgtc aacgcgcagc gcttcgatcc
     1261 gtacaagaac ttcaaattcc gcgtgaaatg ggatggccgc tacgtcgccg gggtctcgaa
     1321 ggtcggcgcg ctcaagcgct cgaccgagat gatcgagcac cgcgaggggg gcgatccgtc
     1381 cacttcgcgc aagtcgccgg gacgcacgaa gtacgaggcg atcacgctcg agcgcggcgt
     1441 cacccacgac agcgagttcg agaagtgggc gaactcggtc tggaactacg gctcgggcct
     1501 cggcgccgag gtgtcgctca agaccttccg caaggacatc atcatcgagg tctataacga
     1561 ggccggccag ctcgtcctcg cctacaaggt ctatcgctgc tgggtatcgg aattccaggc
     1621 gctgcccgac ctcgacgcca acgcgaacgc gatcgcgatc cagacgctca agctcgagaa
     1681 cgagggctgg gagcgcgact acgacgtcac cgagccagcc gagccgcagt tcagcgaacc
     1741 gccgcagtga tgcccgcgct ggacgacgcc gccctgctcg cgctctggga gcgcggcgct
     1801 gcgcttcatc cgctcgatcg cgcgctcctg ctctgcggcg cggcgcgcgc ggacgtcgcg
     1861 cccgcacggc tcgcggacct gccgctcggc gaagcgaacc gcgcgctgct cgagctcagg
     1921 cgcgcgagct tcggtccgca cctcgccgcg ctcgtcgcgt gcgagcgctg cgccgcgccg
     1981 ctcgagatcg agctgcatgc ggacgcgctc gccgcggcac tcggggcgcc gcgcgccgcg
     2041 gcaggcgctt cgctgcgcgc gctcacgatg cgcgatctcg ccgtggtctc tggcgaaccc
     2101 accgccgagg ccgcggcgct tgcgctcgcg cgacgttgcg cgctcgccgg cggccacgac
     2161 gcactgtcgc gcgaggagct cgagcgcggc ctggaagcgc tcgatcccgc ggccgacatc
     2221 gcgctcgaca tgacctgcga ggcttgcggc cacgcctggc gcgcgagcct cgatctcgcc
     2281 gcattcctct ggaccgaagt cgcggcgcgc gcggcggcga ttctcggcga cgttcatcgg
     2341 ctggcgtgcg cctacggctg gaccgagcgc gagatcctcg agctcggccc gcagc
//
LOCUS       DASUNT010000008         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_702443, whole genome shotgun sequence.
ACCESSION   DASUNT010000008 DASUNT010000000
VERSION     DASUNT010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702443"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /gene="lptB"
                     /locus_tag="VFX94_00155"
     CDS             <1..571
                     /gene="lptB"
                     /locus_tag="VFX94_00155"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX5766620.1"
                     /translation="LIPVDSGSIHLDDQELTHLPIHRRARLGISYLPQENSVFRRLTV
                     EENVQAILELQGLTPHDMEPRLAELLKDLNIAHLRKHAAISLSGGERRRLEIARALGT
                     RPAFILLDEPFAGVDPIAVLDIQRIIRFLKERGIGVLITDHNVRETLGICDRAYIINE
                     GAVLASGHPEEIVYNDSVRRVYLGENFRM"
     gene            588..2000
                     /locus_tag="VFX94_00160"
     CDS             588..2000
                     /locus_tag="VFX94_00160"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX5766621.1"
                     /translation="MKPSLQFRLSQHLTLTPQLQQSIRLLQLSTVELNQEIERILMEN
                     PALEREDTEEAPFPAKGSTESPIASATAETPESPDWPSEVAASWRGPDDDEEGDRTHA
                     TPDTPTLRDHLRAQVSLTNLGARDRAFVEILIDALDDDGYLTQPLEEIAALLPPQVAA
                     QSSEYAEALVEELKIALCYLQHFEPAGVGARSPAECLTLQLKTLPDEPQRALALEIVE
                     KHLELLAARDYTRLKALTGAGEDALRAAQRLIQALNPRPGSAFAKVEARYVVPDVIVR
                     KVRNVWRASLNPDAMPRLRINRLYAELAAAARSGAGLSSQLQEARWLIKNVQQRFDTI
                     LRVSQAIVDRQRHFFEHGEVAMRPLVLREIAATLGLHESTISRVTTQKFMATPRGTFE
                     LKYFFGSHVATEAGGAASSTAIRALIKQLVAAENAKAPLSDARISQILGEQGIVVARR
                     TIAKYRESLQIPPVNQRKSL"
     gene            2023..>2260
                     /gene="raiA"
                     /locus_tag="VFX94_00165"
     CDS             2023..>2260
                     /gene="raiA"
                     /locus_tag="VFX94_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX5766622.1"
                     /translation="MNLNISGHHLEVTPALRSYVESKIGRVTRHFDHVIDAHVILTAN
                     KVKQKAEVTLHVRGKDLHCESEEVDLYAAIDLLAD"
BASE COUNT          405 a          802 c          712 g          341 t
ORIGIN      
        1 cctgatcccg gttgactcgg gctcgatcca tctcgatgat caggagctca cgcacttgcc
       61 gatccaccgc cgcgcgcgcc tcggcatctc ctatctgccg caggagaact cggtgttccg
      121 gcgcctcacc gtggaggaga acgtgcaggc gatcctcgag ctccaggggc tcacgccgca
      181 cgacatggag ccgaggctcg cggagctgct gaaggacctg aacatcgcgc acctgcgcaa
      241 gcacgccgcg atctcgctct ccggcggaga gcggcgccgg ctcgagatcg cgcgcgcgct
      301 cggcacgcgg ccggcgttca tcctgctcga cgagcccttc gccggcgtcg acccgatcgc
      361 ggtgctcgac atccagcgca tcattaggtt cctcaaggag cgcggcatcg gcgtgctcat
      421 aacagaccat aacgtgcgcg agaccctcgg gatctgcgat cgcgcgtaca ttatcaacga
      481 aggcgcggtg ctcgcatcgg gccatcccga ggagattgtc tataatgaca gcgttcgccg
      541 agtatatctg ggtgaaaatt tccggatgta gctgagccgc tcaacgaatg aagccgtcgc
      601 ttcaattccg cctctcgcag cacctgaccc tcacgccgca gctgcagcag tcgatccgcc
      661 tgctgcagct ctcgacggtg gagctcaatc aggagatcga gcgcattctc atggagaacc
      721 ccgcgctcga gcgcgaggac accgaagaag cgccgttccc ggcgaaaggc agcaccgagt
      781 cgccgatcgc ttcggccacc gccgagacgc cggagagccc ggactggccg agcgaggtgg
      841 cggcgagctg gcgcggcccc gacgacgacg aggagggcga ccgcacgcac gcgacgcccg
      901 acacgccgac gctgcgcgac catctgcgcg cccaggtctc gctcaccaac ctcggcgcgc
      961 gcgaccgcgc cttcgtcgag atcctgatcg acgcgctgga cgacgatggc tatctcacgc
     1021 agccgctcga ggagatcgcg gctctgctgc caccgcaagt cgccgcgcag tcctccgaat
     1081 atgccgaggc gctcgtcgag gagctgaaga tcgcgctgtg ctacctgcag cacttcgagc
     1141 ccgcgggcgt cggcgcgcgc tcgccggcgg agtgcctcac gctccagctg aagacccttc
     1201 ccgacgagcc gcagcgcgcg cttgcgctcg agatcgtcga gaagcacctc gagcttctcg
     1261 ccgcgcgcga ctacactcgg ctcaaggccc tcaccggcgc gggcgaggac gcgctgcgcg
     1321 cggcgcagcg tctcatccag gcgctcaacc cgcgccccgg ctccgcgttc gccaaggtcg
     1381 aggcgcgcta cgtggtgccc gacgtgatcg tcaggaaggt acgcaacgtg tggcgcgcga
     1441 gcctcaaccc ggacgcgatg ccgcgcctgc gcatcaaccg cctctatgcc gagcttgcgg
     1501 ccgccgcgcg cagcggcgcg ggcctgtcct cgcagctgca ggaggcgcgc tggctgatca
     1561 agaacgtgca gcagcgcttc gacacgatcc tgcgcgtgtc gcaggcgatc gtcgaccgcc
     1621 agcgccactt cttcgagcac ggcgaagtgg cgatgcggcc gctcgtgctg cgcgagatcg
     1681 cggcgacgct cggcctgcac gagagcacaa tctcgcgcgt cactacgcag aagttcatgg
     1741 ccaccccgcg cggcacgttc gagctcaagt actttttcgg cagccatgtc gccaccgagg
     1801 ccggaggcgc cgcctcctcc accgcgatcc gcgcgctcat caagcagctc gtcgcggccg
     1861 aaaacgccaa ggcgccgctt tcggacgcgc gcatttcgca gatcctgggc gagcagggca
     1921 tcgtggtggc gcgccgcacg attgcaaagt acagggagtc gttgcagatt cctcccgtga
     1981 accagcgcaa gtccctgtaa tcatgaaccg tgggggtaca tcttgaacct caatatcagc
     2041 ggccaccacc tcgaagtgac ccccgcgcta cgcagctacg tcgaatcgaa gatcggccgg
     2101 gttacccgcc acttcgacca cgtgatcgac gcgcacgtca tcctcaccgc gaacaaggta
     2161 aagcagaagg ccgaggtcac gctccacgtg cgcggcaagg acctgcactg cgaaagcgag
     2221 gaggtggacc tctacgccgc gattgacttg ctggctgaca
//
LOCUS       DASUNT010000009         2288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_877904, whole genome shotgun sequence.
ACCESSION   DASUNT010000009 DASUNT010000000
VERSION     DASUNT010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877904"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFX94_00170"
     CDS             complement(<1..509)
                     /locus_tag="VFX94_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766623.1"
                     /translation="MKFSIELRDGYLHATVYNRDTADEMREFLVAVHAACRKHGSSKI
                     LLAVRRSRAMFKPEDYGLSAENRGYANELVTPACQIALLGDTDEVNSANEYIELVARQ
                     QGVNARAFRDERAAQRWLGEVGDRPARRYRFTRIVISGAPEEAGVYALWDADELVYYG
                     RADGKNRGGG"
     gene            complement(506..769)
                     /locus_tag="VFX94_00175"
     CDS             complement(506..769)
                     /locus_tag="VFX94_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766624.1"
                     /translation="MEFESLLEALAGLAAANIVLIETLHEQRVVDKSHFAHAFARALD
                     TLAPEHRGGMIEHVLANMRDRCMNLPAGAPSDAREWLRQLLDQ"
     gene            complement(778..>2288)
                     /locus_tag="VFX94_00180"
     CDS             complement(778..>2288)
                     /locus_tag="VFX94_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5766625.1"
                     /translation="LHQSQSLTAELQSQQEELKKTNDRLETQAQNLTKSETLLKGKQD
                     ELRSANEQLQEKARQLSEQMREVEFKNREIEQARAALEEKAEQLSLSSRYKSEFLANM
                     SHELRTPLNSLLILARLLADNAGGNLTPKQVEFARTIHSSGAELLALINDILDLAKIE
                     SGTVTLNIAAEPFAELADTVERTFRQVAADKGLDFHIALGAGLPEAIHTDGKRLQQIL
                     ANLLSNAFKFTAKGEVRLRIEAARSGWSMGHALLDRAAPAIAFSVCDTGIGIPATKQR
                     LIFEAFQQADGSTSREYGGTGLGLSISRELARLLGGEIRVASTPGAGSTFTLYLPAEH
                     HLSAEAAANAHNRGAARGEPALRASEELPAAAAPGASLAPTLAGRQVLVVDDDIRNVF
                     AMTSALELHGITVLHADSGREAVEALAHERGVEAVLMDVMMPGLDGLDTIGLVRALEG
                     YGGVPIIAVTANAMRGDREKCLAAGANEYLAKPVNVDALLAALAKAVRAPAA"
BASE COUNT          309 a          803 c          783 g          393 t
ORIGIN      
        1 ccgccgcccc ggttcttccc gtcggcgcgg ccgtaataga cgagctcgtc ggcgtcccac
       61 agcgcgtaca caccggcttc ttccggcgcg cccgagatca cgatgcgcgt aaagcggtag
      121 cggcgcgccg ggcggtcccc cacctcgccc agccagcgct gcgccgcccg ctcgtcgcgg
      181 aaagcgcgcg cattcacgcc ctgctgacgt gcgacgagtt cgatgtactc gttcgcgctg
      241 ttcacttcgt cggtgtcgcc cagcagcgcg atctggcacg cgggcgtgac gagctcgttc
      301 gcatagccac ggttctccgc cgacaaaccg tagtcctccg gcttgaacat tgcgcgcgag
      361 cgccgcaccg cgagcaggat cttggacgag ccgtgcttgc ggcacgctgc gtgcacagcg
      421 accaggaact cgcgcatttc gtccgcagta tcgcggttgt acacggtggc gtgcaggtag
      481 ccgtcgcgca gctcgatcga gaacttcatt gatcgaggag ctgccgcagc cactcgcgtg
      541 catcggaggg cgcgcccgcg ggcaggttca tgcagcggtc gcgcatgttg gcgagcacgt
      601 gctcgatcat cccgccgcgg tgctcggggg cgagggtgtc cagtgcgcgc gcgaaagcgt
      661 gggcgaagtg actcttgtcc accacgcgct gctcgtgcag cgtctcgatc agcacgatgt
      721 tcgcggccgc gagccccgcc agcgcttcga gcagggactc gaactccatg gagtggctca
      781 cgccgccggc gcccgcaccg ctttcgccag cgccgcgagg agcgcatcca cgttcacggg
      841 cttggcgagg tactcgttgg cgccggccgc gaggcatttt tcgcggtcgc cgcgcatggc
      901 gttggcagtc accgcaatga tcggcacgcc gccgtagccc tcgagtgcgc gcaccaggcc
      961 gatggtgtcg aggccgtcga gccccggcat catcacgtcc atcagcaccg cctcgacgcc
     1021 gcgttcgtgc gccagcgcct ccaccgcctc gcggccgctg tcggcgtgca gcaccgtgat
     1081 cccgtgcagc tcgagcgcgc tcgtcatggc gaacacgttg cggatgtcgt cgtccaccac
     1141 gagcacctgg cgcccggcga gcgtcggcgc gagcgaggcg cccggcgcgg ccgccgcggg
     1201 caattcttcc gacgcgcgca gcgccggctc tcccctcgcc gcgcccctgt tgtgcgcgtt
     1261 cgctgcagcc tccgccgaca ggtgatgctc ggcgggcaga tacagcgtga acgtgcttcc
     1321 tgctcccggc gtgctcgcca cgcggatctc gccgccgagc aggcgcgcga gctcacggct
     1381 gatcgagaga cccaggccgg taccgccgta ctcgcggctc gtggagccgt cggcctgctg
     1441 gaaggcttcg aagatgagcc gctgcttggt cgccgggatg ccgatgccgg tgtcgcagac
     1501 cgagaacgcg atcgccggcg cggcgcggtc gagcagcgcg tggcccatgc tccagcccga
     1561 tcgcgccgcc tcgatgcgca gccgcacttc gcccttcgcg gtgaacttga acgcgttgga
     1621 aagcaggttg gcgaggatct gctgcagccg cttgccgtcg gtgtggatcg cctcgggcag
     1681 gcccgcgccg agcgcgatgt ggaaatcgag ccccttgtcc gccgccacct ggcggaacgt
     1741 gcgctccacg gtgtcggcca gctcggcgaa gggctcggcg gcgatgttga gcgtaaccgt
     1801 ccccgactcg atcttcgcga ggtcaaggat gtcgttgatg agcgcaagga gctccgcgcc
     1861 cgaggagtgg atggtgcgcg cgaactccac ctgcttcggc gtaaggttgc cgccggcgtt
     1921 gtccgcgagg agccgcgcga ggatgagcag gctgttgagc ggcgtcctga gctcgtgcga
     1981 catgttggcg aggaactcgg acttgtagcg cgaggagagc gagagctgct cggccttctc
     2041 ctcgagcgcg gcgcgcgcct gctcgatctc gcggttcttg aactcgacct cgcgcatctg
     2101 ctcggagagc tggcgcgcct tctcctgcag ctgctcgttc gccgagcgca gctcgtcctg
     2161 cttgcccttc agcagggtct ccgacttggt caggttctgc gcctgcgtct cgaggcggtc
     2221 gttggtcttc ttcagctcct cctgctggct ctgcagctcc gcggtcagcg actgcgactg
     2281 gtgcagca
//
LOCUS       DASUNT010000010         2665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_527570, whole genome shotgun sequence.
ACCESSION   DASUNT010000010 DASUNT010000000
VERSION     DASUNT010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527570"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VFX94_00185"
     CDS             complement(<1..291)
                     /locus_tag="VFX94_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX5766626.1"
                     /translation="MKLEVASDVICPWCYIGKKRLEKALGLLKSELTPEMGWLPFQLN
                     PGMPKEGMPRAEYRKAKFGSLERSRELDARVIAEGRSEGIAFAFERIERTPNT"
     gene            complement(292..870)
                     /locus_tag="VFX94_00190"
     CDS             complement(292..870)
                     /locus_tag="VFX94_00190"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5766627.1"
                     /translation="MRILVAVAAAVLAATPAAALDRSPYDFLAQAQPRKPAQQPDVIF
                     VPTPHEVVDDMLRLANVGKDDILYDLGSGDGRIAIAAAKKFGIRAVGIDIDPERIREA
                     NDNARKAGVSHLVQFRQEDLFKADFREATVVTLYLLPDLNVKLRPRLWDELKPGTRII
                     SHQFDMGTWKPEKRLDSNGRVVYFWTVPAKTK"
     gene            complement(1045..1323)
                     /locus_tag="VFX94_00195"
     CDS             complement(1045..1323)
                     /locus_tag="VFX94_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766628.1"
                     /translation="MTVHVFSHHEHPGPHEHPVIAWIAIVAIVAAILLIAWARLEIHG
                     TDDLEAALAETPAQGDLPLAPRTDLKPDEERELDAAVTSSRFATGFSP"
     gene            complement(1364..>2665)
                     /gene="pmbA"
                     /locus_tag="VFX94_00200"
     CDS             complement(1364..>2665)
                     /gene="pmbA"
                     /locus_tag="VFX94_00200"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF008268.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEX5766629.1"
                     /translation="QAVLDRARRAGASGCECDVSEGYGLSVTVRKGKPDTIEHNRDRS
                     IGVTVYFGERPRARRGHASTSDFSRAALEQTVDAAAAIARHTAEDDCAGLPEPELLAR
                     EQPNLDLFHPWDISTEEAVDLARRCEAAAFAVSKKIRNSEGATVSAQQTQFVLANSHG
                     FVGGFAGSRHWISCAVIAEEKGLMQRDDWYSASRVPEKLADARALGRYAGERAAARLG
                     ARKIATCQAPVLFEAPVAIGLIGHFVSAVSGGNLYRKTSFLVDSLGEQVFSPLVSIDE
                     RPRERQGMASSAFDEEGVATIERPIVREGVVEGYFLGSYAARKLGMKSTGSAGGHHNL
                     AVRSEGPDFNGMLKNLGRGLLVTELLGQGVNPVTGDYSRGAAGYWVEGGEVAFPVEEI
                     TIAGNLRQMFRQIAAIGSDVVVRSGRSTGSILVESMTIAGE"
BASE COUNT          400 a          886 c          905 g          474 t
ORIGIN      
        1 cgtgttcggg gtgcgctcga tgcgctcgaa cgcgaacgcg atgccttcgc tgcggccctc
       61 ggcgataacg cgcgcgtcca gctcgcgcga gcgctcgagc gatccgaact tcgccttgcg
      121 gtactcggcg cgcggcatgc cttctttcgg catgccggga ttgagctgga agggaagcca
      181 gcccatttcc ggcgtgagct cgcttttcag caggcccagg gccttttcga gccgcttctt
      241 gccgatgtag caccacggac agatgacgtc cgatgcgact tccagcttca tctatttcgt
      301 cttcgccgga accgtccaga aatagaccac ccggccgttt gagtcgagcc gcttctcggg
      361 cttccaagtg cccatgtcga actggtgcga gatgatgcgc gtgcccggct tgagctcgtc
      421 ccaaagccgc ggccgcagct tcacgttgag gtcgggcagc aggtacagcg taacgacggt
      481 cgcttcgcgg aaatcggcct tgaagaggtc ctcctgccgg aactggacga gatggctgac
      541 gcccgccttg cgcgcgttat cgttcgcctc gcggatacgc tccggatcga tgtcgatgcc
      601 gacagcgcgg atgccaaact tcttcgccgc cgcgatcgcg atgcggccgt cccccgagcc
      661 gaggtcataa aggatgtcgt ccttgcccac gttggcgagg cgcagcatgt cgtccacgac
      721 ctcgtggggc gtagggacaa aaatgacatc gggctgctgc gccggcttgc gcggttgcgc
      781 ctgcgctagg aaatcgtacg gggaccggtc gagcgcggcg gctggggtcg cggcaaggac
      841 cgccgcggca actgctacga ggattcgcat tcgtgcgatt atgccttagg gcattcctga
      901 cataggcgcc ctgcgcggag gtacatcagc gggggcaccc gctagcgttt cagtcggggg
      961 cacccccgag tgtttcagtc ggggggatta ccgctttcgc gggaaggacg acagccgcct
     1021 gcgcttcgct gcgcgcctat ttagttaggg cgagaacccg gtcgcgaaac ggctggaggt
     1081 tacggctgca tcgagctcgc gttcctcgtc gggcttgagg tcggtccggg gcgcgagcgg
     1141 caggtcgccc tgcgccggcg tctcggcgag cgccgcctcc aggtcgtcgg tgccgtggat
     1201 ctccaggcgg gcccacgcga tgagcaggat cgccgcgacg atcgcgacaa tcgcgatcca
     1261 cgcgatcacc ggatgctcgt gcgggcccgg atgctcgtga tggctgaaga catgcaccgt
     1321 catgcttaga cgctactcct cgccgccggt tcaggctagc gaactattca cccgcgatgg
     1381 tcatgctctc gacgaggatc gaaccggtgg aacggccgct gcgcaccacc acgtcgctcc
     1441 ctatggccgc gatttgcctg aacatttgcc gcaggttgcc ggcaattgtt atctcttcta
     1501 ccggaaatgc gacctctccg ccctcgaccc agtagccggc ggcgccgcgc gaatagtcgc
     1561 cggtgaccgg gttgacccct tgcccgagga gctcggtcac gagcagcccg cgcccgaggt
     1621 ttttcagcat tccgttgaag tcgggtcctt cggagcgcac tgcaagattg tgatgtcctc
     1681 cggcgctgcc ggtggatttc atgccgagct tccgggccgc gtagctgccc aggaagtagc
     1741 cctccaccac gccttcgcgc acgatcggcc gctcgatcgt cgccacgcct tcctcgtcga
     1801 aggcgctcga ggccatgccc tgccgctcgc gcgggcgctc gtcgatggag acgagcggcg
     1861 aaaagacctg ctcgccgagg ctgtcgacga ggaacgaggt cttgcggtag aggttccctc
     1921 cgctcaccgc ggacacgaaa tggccgatga gcccgatcgc caccggcgct tcgaagagca
     1981 ccggcgcctg gcaggtggcg atcttgcgag cgcccaggcg cgcggcggcc cgctcgccgg
     2041 cgtagcgccc gagcgcgcgc gcatcggcca gcttttccgg tacgcgggag gcgctgtacc
     2101 agtcgtcgcg ctgcatcagg cctttttcct cggcgatcac cgcgcacgat atccagtggc
     2161 gcgagccggc gaagccgccg acgaagccgt ggctgtttgc gagcacgaat tgcgtctgct
     2221 gcgcggagac ggtcgcgccc tcggagttgc ggatcttctt cgaaaccgcg aacgccgcgg
     2281 cctcgcagcg gcgcgcgagg tccaccgcct cctcggtgga aatgtcccag gggtggaaga
     2341 ggtcgaggtt cggttgctcg cgggcaagga gctcgggctc cgggagcccg gcgcaatcgt
     2401 cctcggcggt gtggcgcgcg atcgccgcgg cggcgtcgac cgtctgctcg agcgcggcgc
     2461 gcgagaagtc cgaggtgctg gcgtgccccc gccttgcgcg agggcgctcg ccgaaataca
     2521 ccgtgacgcc gatggagcgg tcgcggttgt gctcgatggt gtcgggcttg cccttgcgca
     2581 ccgtgacgct caggccgtag ccctcggaca cgtcgcactc gcagccgctc gcgccggcgc
     2641 ggcgcgcgcg atcgagcacg gcctg
//
LOCUS       DASUNT010000011         2916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_703187, whole genome shotgun sequence.
ACCESSION   DASUNT010000011 DASUNT010000000
VERSION     DASUNT010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703187"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VFX94_00205"
     CDS             complement(<1..960)
                     /locus_tag="VFX94_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX5766630.1"
                     /translation="MKKLLVLALLYAPAALAQAPAAAPKIDAANTGWMLTASALVLFM
                     TLPGLALFYAGLVRTKNVLSVLMQCFAITCVVTLAWLALGYSVAFGDGNAWWGGLGKS
                     FLSGIQASTVKGSIPETVFAMYQLTFAIITPALVVGAYAERVRFGGMLVFSALWLLLV
                     YCPVAHWVWGEGWLQKRGLMDFAGGTVVHLNAGVAALVCAVVLGRRRGFPETAMPPHN
                     MTMAVTGAAMLWFGWFGFNAGSALAADGTAGMAMLVTHIGAATGASAWMVCEWLRYGK
                     PSALGIVTGMVAGLGTITPASGFVGPLGALVIGAVAGVACFFAT"
     gene            complement(987..1514)
                     /locus_tag="VFX94_00210"
     CDS             complement(987..1514)
                     /locus_tag="VFX94_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5766631.1"
                     /translation="MKFCSNCASPVVKRVPPGDTLARWVCDQCGEIHYQNPRLVVGSI
                     PEYEGKLLLCRRAIEPRYGYWTLPAGFMENDETTAQAAARETLEEAGARVELEAPFSM
                     ISVPRVNQVHLFYRARVMELGFTPGEESLEVALWEEAKIPWRDIAFRTVALTLQHWFA
                     DRGNGAFGFHAEDLQ"
     gene            complement(1498..2142)
                     /locus_tag="VFX94_00215"
     CDS             complement(1498..2142)
                     /locus_tag="VFX94_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX5766632.1"
                     /translation="MAAEAMVFDAYGTLFDVHSVAARCETCWPGKGGQLSQLWRAKQL
                     EYTWQRSLMQRYAPFSAVTREALAYACEALGLELDAPRTALLMEEYLKLATYPDVAAT
                     LKKLGGRQAILSNGSPDMLLPLVEHSGLRFDALISVDQLRVYKPAPQVYELAANKLNA
                     DKSTIGFVSSNCWDAMGAKSFGFKVYWINRAQAPVDRLGFRPDAILRSLDEVLQ"
     gene            2166..>2916
                     /locus_tag="VFX94_00220"
     CDS             2166..>2916
                     /locus_tag="VFX94_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5766633.1"
                     /translation="MPDRYEELYRSFRWDVPERYNMAQACCGKWALDRARFALYWEDD
                     SGATAAYSFWDIQQAANRLSNALAALGVKRGERVAIILPQRPQTAIAYIAVFQMGAIA
                     LPLSHLFGPDALEYRMEHAEASVAIVEPTTIANLLAIRDRLTHLRHVIAVDSREKGVL
                     CWAKLLEKASARFAPVDTRADDPALIIYTSGTTGPPKGALKAHRVLIGNLPGFVHSHD
                     FFPQPGDMFWSPADWAWTGGLFDALLPTWHFG"
BASE COUNT          509 a         1016 c          934 g          457 t
ORIGIN      
        1 cgtggcgaag aagcaggcga ccccggcgac cgcgccgatg acgagcgcgc cgagcggccc
       61 gacgaagccc gaggccggcg tgatggttcc gagccccgcc accatcccgg tcacgatgcc
      121 gagcgccgaa ggcttgccgt agcgcagcca ttcgcacacc atccaggcgg aagcgccggt
      181 cgccgcgccg atgtgcgtaa ccagcatcgc catgccggcg gtgccgtcgg cggcgagcgc
      241 gctgcccgcg ttgaacccga accagccgaa ccacagcatg gccgcgccgg tcaccgccat
      301 ggtcatgtta tgcggcggca ttgctgtttc cggaaagccg cggcgccggc caaggacgac
      361 cgcgcaaacg agcgctgcca cgcccgcgtt aaggtgcacc accgtgccgc cggcgaagtc
      421 catcaggccg cgcttctgca gccagccctc gccccacacc caatgcgcca ccgggcagta
      481 cacgaggagc agccagaggg cgctgaagac cagcattccg ccgaagcgca cgcgctccgc
      541 gtaggccccc accacgagcg ccggtgtaat gatggcgaag gtgagctggt acatcgcgaa
      601 caccgtctcc gggatgctgc ctttcaccgt gctcgcctgg ataccggaaa ggaagctctt
      661 gcccaggccg ccccaccagg cgttgccgtc gccgaacgcg acgctgtagc cgagcgcgag
      721 ccaggcgagc gtgaccacgc acgtgatcgc gaagcactgc atcaggaccg acaagacgtt
      781 cttcgtgcgc accagtcccg cgtagaaaag cgcaagaccg ggcagcgtca tgaaaagcac
      841 gagcgccgag gcggtcagca tccagccggt attcgccgcg tcgatcttcg gcgcggccgc
      901 cggcgcctgc gccagcgcag ccggcgcgta aagcaaggcc aggacgagca attttttcat
      961 tcgagcctcc cctcgttgtt ggttttttat tgtaggtcct ccgcatggaa gccgaaagcg
     1021 ccgttgcccc gatcggcaaa ccagtgctgg agcgtgagcg cgacggtgcg gaacgcgatg
     1081 tcgcgccatg ggattttcgc ctcctcccac aacgccacct cgaggctttc ctccccgggc
     1141 gtgaacccga gctccatcac gcgcgcgcgg tagaagaggt gcacctggtt tactcgcggc
     1201 acggaaatca tgctgaacgg cgcttccagc tcgacgcgcg caccggcctc ttccagcgtt
     1261 tcgcgcgccg ccgcctgggc ggtggtctcg tcgttctcca taaagcccgc ggggagcgtc
     1321 cagtagccgt agcgaggctc gatcgcccgg cggcagagga gcagcttgcc ctcgtattcg
     1381 gggatcgagc ccaccaccag gcgcggattc tggtagtgga tctcgccgca ctggtcgcag
     1441 acccagcgcg ccaacgtgtc gcccggcggc acccgcttca ctaccggcga ggcgcagtta
     1501 ctgcagaact tcatcgagcg agcgcaggat cgcgtccggc ctgaacccga ggcggtccac
     1561 gggcgcttgc gcgcggttga tccagtaaac cttgaagccg aacgacttcg cgcccatggc
     1621 atcccagcag tttgaggaca cgaagccgat tgtgcttttg tccgcattaa gcttgttcgc
     1681 cgcaagctcg tagacttgcg gagccggctt gtagaccctg agctggtcca cactgatgag
     1741 cgcgtcgaac cgcagccccg agtgctctac gagcggaagc agcatgtccg gggaaccgtt
     1801 ggagagaatc gcctgccttc cccccagctt cttgagcgtt gcggccacat caggataggt
     1861 ggcgagcttc aggtactcct ccatgaggag cgccgtgcgc ggtgcatcta gctccaggcc
     1921 cagcgcctcg cacgcatagg caagcgcctc gcgcgtcacc gcggagaagg gcgcgtaacg
     1981 ctgcatcagg ctgcgctgcc aggtgtactc gagctgcttt gcgcgccaga gctgcgagag
     2041 ctggccgcct ttcccgggcc agcaggtctc gcagcgcgcg gcgaccgagt gcacgtcgaa
     2101 gagcgtgccg tacgcatcga agaccatcgc ctcggccgcc atggccgcaa gtatagaatc
     2161 cccgcatgcc cgaccgctac gaggagctct accggagctt ccgctgggac gttcccgagc
     2221 gctacaacat ggcgcaagcg tgctgtggca agtgggcgct cgaccgcgcg cgcttcgcgc
     2281 tctactggga ggacgactcg ggcgccaccg cggcgtactc gttctgggac atccagcagg
     2341 cggcaaaccg gctctcgaac gcgctcgccg ccctgggcgt aaagcgcggc gagcgcgtgg
     2401 cgatcatctt gccgcagcgg ccgcagaccg cgatcgccta catcgcggta ttccagatgg
     2461 gcgccatcgc gctgccgctg tcgcatctct tcgggcccga cgcgctcgag taccggatgg
     2521 agcatgccga ggcctcggtc gcgatcgtcg agccgaccac gatcgccaac ctcctggcga
     2581 tccgcgatcg cctgacccat ctgcggcacg tcatagcagt agacagccgc gaaaagggcg
     2641 tgctttgctg ggcgaagctg ctcgaaaagg cgagcgcgcg tttcgcgccc gtggataccc
     2701 gggccgacga tccggcgctg atcatctata caagcggcac gaccggtccg ccaaagggcg
     2761 cactgaaggc gcaccgcgtt ctcatcggca acctcccggg gttcgtgcat tcgcacgact
     2821 tcttccccca gcctggcgac atgttctggt cgccggccga ctgggcctgg accggcggtc
     2881 tgttcgatgc gctgctgccc acctggcact tcggtt
//
LOCUS       DASUNT010000012         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_177235, whole genome shotgun sequence.
ACCESSION   DASUNT010000012 DASUNT010000000
VERSION     DASUNT010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177235"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFX94_00225"
     CDS             <1..288
                     /locus_tag="VFX94_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX5766634.1"
                     /translation="DVAVCANDWCLAPDSSLDDGRVGALVSSYHAARPFSSLEVQAWR
                     PMLRAAALRFWLSRLYDFHLPRPGMLVHAHDPEHFRRVLESRIAVEAPWIA"
     gene            288..1055
                     /locus_tag="VFX94_00230"
     CDS             288..1055
                     /locus_tag="VFX94_00230"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HEX5766635.1"
                     /translation="MQARIVQALRGALWLAEGWRLFSAAPLGWIAMVFAYWLLMTLAS
                     IVPVLGVAAASVLVPAFSVGFMAAARSAARKGPVELALLFDGFRNHLRSQLVLGVAYF
                     ACLALVLAATVLVDEGELAGWMLTGSRPEDEVLRSERFVAALAAAAALYTPVLMMFWF
                     APPLAAWHGVAPAKALFFSFFACLMNWRAFLAYGAVTALVALALPLALVSVVALGSLN
                     VPAMSLVFPLLIVLLPTLFASFYASYRDVFGAEGARA"
     gene            1052..>2480
                     /locus_tag="VFX94_00235"
     CDS             1052..>2480
                     /locus_tag="VFX94_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HEX5766636.1"
                     /translation="MNRIREIPYNYTSFSDREIALRLLGAEGWQLISELRAERRTGRS
                     ARMLYEVLGEMWAIQRNPYLEDELVNRPQRRAEALGGMRRRLAEIDARRAGAPKVARL
                     LELARGAVAAFENDLAATRALRSTLLRRLGRFTRRDNILFDGFARVSHCTDATDWRVE
                     MPFVVLAPDTEEEIQPLVRELAALDLTIIPRGGGTGYTGGAVPLTRRSAVINTEKLET
                     LGEVEHTELPGVARAQPTIRASAGVVTRRVMEAAEAGGLVFAMDPTSADASCIGGNVA
                     MNAGGKKAVLWGTAIDNLVWWRMVTPRGEWLEAERLEHNLGKIHDAPSAKFRLRFFEK
                     KRHLREEVLDIPGAHFRKAGLGKDVSDKFLAGLPGVQKEGTDGIIVAARFLLHRMPPH
                     TRSVCLEFFGLARDAVPAIVEIKNYVQKSHAVMLAGLEHLDERYVKAVGYATKSRRSE
                     RPRMVLIGDLVGEDVDAVAAAASGVV"
BASE COUNT          329 a          859 c          870 g          422 t
ORIGIN      
        1 gacgtggccg tatgcgcgaa cgactggtgc ctcgcgccgg acagctcgct cgacgatggc
       61 cgcgtcgggg cgctcgtctc cagctaccac gccgcgcggc cgttcagctc gctcgaggtc
      121 caggcctggc ggccgatgtt gcgcgcggcg gcgctgcgct tctggctctc gcggctctat
      181 gacttccacc tcccgcgccc ggggatgctg gtgcatgcgc acgaccccga gcatttccgc
      241 cgcgtgctgg agtcccgcat cgccgtagaa gcgccctgga tcgcctgatg caggcgcgca
      301 tcgtgcaggc gttgcggggg gcgctctggc ttgccgaagg gtggcggctc ttcagcgccg
      361 cgccgctcgg gtggatcgcg atggtgttcg cgtactggct gctgatgacg ctggcgtcca
      421 tcgttccggt gctcggcgtc gccgcggcat cggtcctggt gccggcgttt tcggtcggct
      481 tcatggccgc ggcgcgctcg gctgcgcgca agggaccggt ggagctcgcg ctccttttcg
      541 atggctttcg caatcacctg cgcagccagc tcgtcctggg cgtcgcctac ttcgcctgcc
      601 tggcgctcgt gctggccgcg acggtgctcg tggacgaggg cgagctcgcg ggatggatgc
      661 tcaccgggag ccggcccgag gacgaggtgc tgcgctccga gcggttcgtc gctgcgctcg
      721 ccgccgcggc cgctctgtat accccggtcc tcatgatgtt ctggttcgcg ccgccgctcg
      781 ccgcctggca cggcgtggcg cccgccaagg cgctcttctt cagctttttc gcctgcctga
      841 tgaactggcg tgctttcctc gcctacggcg ccgtgaccgc gctcgtcgcg ctcgcgctgc
      901 cgctcgcact cgtcagcgtc gtggcgctcg gctcgctcaa tgtgccggcc atgtcgctcg
      961 tgttcccgct gcttatcgtt ctgctcccga cgctctttgc cagtttctac gcgagctatc
     1021 gcgacgtctt cggcgcggag ggcgcgcgag cgtgaaccgc atccgcgaga tcccgtacaa
     1081 ctacacctcg ttctcggacc gcgagatcgc gctgcggctg ctgggcgccg agggctggca
     1141 gctcatcagc gagctgcgcg ccgagcgccg gaccgggcgc tcggcccgca tgctctacga
     1201 ggtgctcggc gagatgtggg ccatccagcg caacccctac ctcgaggacg agctggtcaa
     1261 ccggccgcag cgccgggccg aagcgctcgg cggcatgcgc cggcggctgg ccgagatcga
     1321 tgcacggcga gccggcgcgc ccaaggtggc gcggcttctc gagctcgcgc gcggcgccgt
     1381 cgcagcgttc gagaacgacc tcgcagcgac ccgcgcgctg cgctccacgc tcctgcgccg
     1441 gctcggacgc ttcacgcggc gcgacaacat cctcttcgac ggcttcgcgc gcgtctcgca
     1501 ttgcaccgac gcgaccgact ggcgcgtgga gatgccgttc gttgtcctcg ctccggacac
     1561 cgaggaagag attcagccgc tcgtgcggga gctcgccgcc ctggatctca ccatcattcc
     1621 gcgcggcggc gggacaggct acacgggagg cgcggtgccg ctcacgcgcc gctcggcggt
     1681 catcaacacc gagaagctcg agacgctcgg cgaagtcgag cacacggagc tcccgggcgt
     1741 ggcgcgcgcg caaccgacga tccgcgcctc ggccggggtc gtgacccggc gggtcatgga
     1801 ggccgccgag gcgggcggtc tcgtgttcgc aatggacccg acttcggcag acgcctcgtg
     1861 catcggtggc aacgtggcca tgaacgcggg cgggaagaaa gccgtgctgt ggggcacggc
     1921 aatcgacaac ctcgtatggt ggcgcatggt caccccgcgc ggcgagtggc tggaagccga
     1981 gcgcctggag cacaacctcg gcaagatcca cgacgcgccg agcgcgaagt tcagattgcg
     2041 gttcttcgaa aagaaaaggc atctgcggga agaagttctc gacattcccg gcgcgcattt
     2101 ccgcaaggcg gggctgggca aggacgtgag cgacaagttc ctcgccggcc tgccgggggt
     2161 gcagaaggag ggcaccgacg gcatcatcgt cgctgcgcgc tttctgcttc accgcatgcc
     2221 gccgcacacg cgcagcgtgt gcctcgagtt cttcggcctg gcacgtgacg ccgtgccggc
     2281 catcgtggaa ataaagaact acgtgcaaaa atcccatgcc gtcatgcttg cggggctcga
     2341 gcatctggac gagcgctacg taaaggcggt gggctacgcc accaagtcgc gccgcagcga
     2401 gcgcccgcgc atggtgctga tcggtgacct cgtgggggag gacgtcgacg cggtcgccgc
     2461 tgcggcttcg ggcgtcgtgc
//
LOCUS       DASUNT010000013         3324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_89683, whole genome shotgun sequence.
ACCESSION   DASUNT010000013 DASUNT010000000
VERSION     DASUNT010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89683"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..913
                     /locus_tag="VFX94_00240"
                     /pseudo
     CDS             119..913
                     /locus_tag="VFX94_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            1184..1366
                     /locus_tag="VFX94_00245"
                     /pseudo
     CDS             1184..1366
                     /locus_tag="VFX94_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524925.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1363..1461)
                     /locus_tag="VFX94_00250"
     CDS             complement(1363..1461)
                     /locus_tag="VFX94_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HEX5766637.1"
                     /translation="MGKGDKRTRRGKIYRGSYGKKRPQRQPNRKKS"
     gene            complement(1473..>3324)
                     /locus_tag="VFX94_00255"
     CDS             complement(1473..>3324)
                     /locus_tag="VFX94_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEX5766638.1"
                     /translation="ATERLIEIAEAHKTGGEKRKEDDLAWRNAPAVERLKHALVKGID
                     AFVEADTEEARRDTVARGGRTIEVIEGPLMDGMNVVGDLFGAGKMFLPQVVKSARVMK
                     QAVAHLVPYIEAEKAKSGEAGRAKGRLVLATVKGDVHDIGKNIVAVVLQCNNYEVLNL
                     GVMVPAEAILETARREQADVIGLSGLITPSLEEMAHVAREMERAGLKLPLLIGGATTS
                     RAHTAVKIAPNYSGPVVYVPDASRSVPVVQSLLGESREAYLAEVRSDYERIRVQHRDK
                     KGPGPLHPLAEARSRGYKGDWRNYVPPAPARPGLTVLRRYPLAELVQYIDWSPFFQAW
                     ELSGPYPKILDDPVVGAAARKLFAEAEEALARIVRENLIEANGVVALYPASQVDCDDI
                     EIYADEEGRQRLMTWHNLRQQNHKPEGRANLCLADFVAPKASGVRDWIGAFAVCAGGI
                     EERVGELEKRGDDYNAIMLKVLADRLAEAFAERLHERVRREFWGYAAEERLTAPELIA
                     ERYRGIRPAPGYPACPDHTAKGPLFRLLDAEANAGMRLTESFAMLPASSVSGFYLSHP
                     EASYFAVGKIGRDQLEDYARRSGAGVPEAEKWLAPYLAYEPQGAAASASL"
BASE COUNT          486 a         1157 c         1110 g          571 t
ORIGIN      
        1 cctcgcgatc gtgaaaaaga tcgtggacga gcaccacggc gcggtgacga tcgagaaccg
       61 gccggcgagc ggcgcatcgg tgagcgtgct gctgccgctc gccaaggcgg cctaggcgat
      121 ggcgcagatc ctggtcgtcg acgacgagat cggcatccgc gagctgctgt cggagatcct
      181 cgccgacgag ggccaccagg tggtcctcgc cgagagcgcg agcgacgcgc gccggctgcg
      241 cgagcgcgcg cgcccggacc tcgtcctgct tgacatctgg atgccggacg ccgacggcat
      301 cacgcttctg aaggagtggg cggtgagcgg ccagctcacc atgccggtcg tgatgatgtc
      361 cggccatggc acgatcgaga ccgcggtcga ggcgacgcgc atcggcgcgc tcgacttcct
      421 cgagaagccg atcgcgctgc agcgccttct cgccacggtg aagcgcgccc tgcgtcatcc
      481 ggaaaacgcg gcggcgccgg agttttccat cgcagcgctc gggcgcgcgc ccgcgctcgt
      541 ggaggtgaag aagcgcctcg gccagctcgc cccctcggtc gcgccgctga tgctgcgcgg
      601 ggagcgcggc atccggccgg agctcttcgc ccgcctgctc gtgccggcgg gagcgccatt
      661 cctctccggc ggcgagatgc tgtcgcagcc gccctccgag ctcctcgcca aggcggccgg
      721 aggcgtgctt ttcatccccg atctcagccg cctcgggcac gcggagcagc gcaacctcga
      781 cttcctgctc gctcgcgccg agaagcaccg cgtgcgcgtg gtgtcgttct ctcctctgga
      841 cgtgcgcacg ctggcggaga aatgcgaatt cgatcccgag ctcggcgccc ggctcggcga
      901 gctcacgctc aagcttccgg cgctgcgcga gttcgccgag gacgtgcccg acctcgcgtc
      961 gtacgtgctc gcccaactgg tggaggcgcg gacctgcccg ccgcgccgct tcgctgtcgc
     1021 agcgttgaac gcgctgcgcc accacggctg gccgggaaat cttgccgagc tcgagagcgt
     1081 cgtcaaggac cttgcgctca gctcgctgga ggaggagatc cagctcgtgg acgtcgggcg
     1141 cgtcctgaac gcgcgcgcgg ccccgggcac actgcccgaa atcgcgttcg accggccgta
     1201 tcgcgaggca cgcgaggctt ttgagcgggt ctatttcgag cacctgctcg cgcgcgagaa
     1261 cggcagcatg tcacgcgtgg cagagcgctc ggggctggag cgcacccacc tctaccgcaa
     1321 gctgaaggcg ctgggcctgc cggtcggcag acgcgaggaa acctagcttt tctttcggtt
     1381 cggctggcgc tgcggccgct tcttgccgta gctgccgcgg taaatcttgc cgcgccgcgt
     1441 gcgcttatcg ccctttccca tggcctcgga ttctataagg aagcgcttgc cgctgcgcct
     1501 tgcggctcat atgcgaggta cggcgcaagc catttctccg cttcgggcac acccgcgccg
     1561 ctacgccgcg cgtaatcctc gagctggtcg cgcccgatct tgccgacggc gaagtagctc
     1621 gcctccgggt gggagagata gaaccccgac accgaggaag cgggcagcat ggcgaacgac
     1681 tcggtgagcc gcatgccggc gttggcttcc gcgtcgagca ggcgaaacaa gggtcccttg
     1741 gcagtgtggt ccgggcacgc cggatatccg ggggcggggc ggattccccg atagcgctcg
     1801 gcaatgagct ccggagcggt caaccgctct tcggcggcat agccccagaa ctcccgccgc
     1861 acccgttcgt ggagccgctc ggcaaaggcc tccgccaggc ggtcggcgag cactttgagc
     1921 atgatcgcgt tgtagtcgtc gccgcgcttt tccagctcgc cgacgcgctc ttcgatcccg
     1981 ccggcgcaca cggcaaacgc cccgatccag tcgcgcaccc cgctcgcctt gggtgcgacg
     2041 aaatccgcga ggcagagatt cgcccgaccc tcgggcttgt ggttctgctg ccgcaagttg
     2101 tgccaggtca tgagccgctg gcggccctct tcgtcggcat agatctcgat atcgtcgcag
     2161 tcgacctgag acgcggggta aagcgcaacc acgccgttcg cctcgatgag gttctcgcgc
     2221 acgatgcgcg caagcgcttc ctcggcctcc gcgaaaagct tgcgcgcggc cgcgcccacg
     2281 accgggtcat cgaggatctt cggatacggt cctgacaatt cccaggcctg gaagaagggg
     2341 ctccagtcga tgtactggac cagctccgcc agcggatagc ggcgcaggac cgtgagtccc
     2401 ggcctggctg gcgctggcgg gacgtagtta cgccaatcgc ccttgtaacc gcggctgcgt
     2461 gcttcggcga gcgggtgtaa cggcccgggg cctttcttgt cacggtgctg tacgcgaatg
     2521 cgctcgtaat cggagcgcac ctccgcgagg taggcctcgc ggctttcccc gagcaggctt
     2581 tgcactaccg ggacgctgcg cgaggcgtcc ggcacgtaca ccaccggacc ggaatagttt
     2641 ggcgcaatct tcaccgcggt gtgcgcgcgc gaggtcgtcg cgccgccgat gagcagcggg
     2701 agcttcagtc cggcgcgctc catctcgcga gcgacatgcg ccatttcctc gagcgaggga
     2761 gtaatcagtc ccgaaaggcc gatcacgtcc gcctgctcgc ggcgtgcggt ttcgagaatc
     2821 gcctcggccg ggaccatcac gccgaggttc agcacctcgt agttgttgca ctggagaacg
     2881 acggctacga tattcttgcc gatgtcgtgc acgtcgccct tcaccgtggc gagcacgagc
     2941 cgtcctttgg cgcggccggc ttcgccgctt ttcgctttct cggcttcgat gtacggcacg
     3001 aggtgcgcca ccgcctgctt catgacgcgc gccgatttca cgacttgcgg caggaacatc
     3061 ttgcctgcgc cgaaaaggtc acccaccacg ttcatgccgt ccatcagcgg cccctcgatg
     3121 acctcgatcg tgcggccgcc gcgggccacg gtgtcgcggc gcgcttcctc ggtgtcggcc
     3181 tcgacgaacg cgtcgatgcc cttgaccagc gcgtgcttca gccgctcgac cgccggcgca
     3241 ttgcgccagg ccagatcgtc ctccttgcgc ttctcgccgc cggtcttgtg cgcctcggcg
     3301 atctcgatga ggcgctcggt ggcg
//
LOCUS       DASUNT010000014         2686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_177415, whole genome shotgun sequence.
ACCESSION   DASUNT010000014 DASUNT010000000
VERSION     DASUNT010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177415"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..574
                     /locus_tag="VFX94_00260"
     CDS             287..574
                     /locus_tag="VFX94_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEX5766639.1"
                     /translation="MAKKKAKKSGKKRKPNAAFMKAMTPSGPLASVVGSGAMPRTEVT
                     KKIWGYIKRNGLQDKKNRRMINADDKLKAVFGGKGQVSMFEMTKLVSRHLK"
     gene            complement(626..1822)
                     /locus_tag="VFX94_00265"
                     /pseudo
     CDS             complement(626..1822)
                     /locus_tag="VFX94_00265"
                     /inference="COORDINATES: protein motif:HMM:NF006779.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
     gene            complement(1717..2646)
                     /locus_tag="VFX94_00270"
                     /pseudo
     CDS             complement(1717..2646)
                     /locus_tag="VFX94_00270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          532 a          816 c          933 g          405 t
ORIGIN      
        1 cggatcggga tcggcctgcg cttccgagag cccgcagcgc atgtattcct ggcgcagatc
       61 ggcgatcttc atgcgcgcga ttctacgtcc gcggcgtggc gcgggcccga ctacccgggc
      121 aacgagtcgc actttcccta tgaaaagcgt tgacacgcga tagcttcttc ggcatcttca
      181 cgacaccgct gcgcttgtgg cgggggaacc gggagccgaa gtcagcagga aagtcggcaa
      241 gctcgtcagc atttccaaat agatcgttca gaggagaaac agagacatgg cgaagaaaaa
      301 agcgaagaag tccggcaaga agcgcaagcc gaatgccgcc ttcatgaagg cgatgacgcc
      361 gagcggtccg ctcgcgtccg tggtcggctc gggcgcgatg ccgcgcaccg aggtgaccaa
      421 gaagatctgg ggctacatca agcgcaacgg gctacaggac aagaagaacc gtcgcatgat
      481 caacgccgac gacaagctca aggccgtgtt cggcggcaag ggccaggtgt cgatgttcga
      541 gatgaccaag ctggtcagcc gccacctgaa ataacggttt cccgaggagg acggaacgaa
      601 gcggggcccc tgcgcggggc cccgctcatt tccggtggta cgaggtgacg cggtcgactt
      661 cgttcctcga gccgaggatc accgcgcagc gctggtgcag ccccttgggc tggacatcga
      721 ggatcgggat ctcgccgtcg gtcgccgcgc cgcccgcctg ctcgatgatg aacgccatcg
      781 ggttcgcctc gtacatgagg cgcaggcgcc cttccttgtt cttcgagtcg cgcgggtaca
      841 tgaagatgcc gccgcgcgag agcacgcgga agacgtcggc caccatggaa gcgacccagc
      901 gcatgttgaa atccttgccg cgaggcccct ccttgccgac gaggcattcc tcgatgtagc
      961 gcttgaccgg cggttcccag cgccttgcgt tcgaggcgtt gatcgcgaac tcctgcgtgt
     1021 cttccgggat gcggattgcc gcgtgggtct ggacgaacgt gtaggtctcg cggtccaggg
     1081 tgaagccgta gacgccgtcc ccgaccgtga tgacgaacac cgtggtgggc ccgtataccg
     1141 cgaaaccggc cgcgatctgc ttgcgcccgg gctgcaggaa cgcctgctcg ttcgcgtcgc
     1201 agacgctgcc gtcggcattc ttcgggcagc gcagcaccga gaagatcgtg ccgaccgaga
     1261 tgttgacgtc gatgttggac gatccgtcga gcgggtcgaa gagcaggaga tactcgccgc
     1321 gcgggtagtg cgtcgggatg gggcgcggct cgtccatttc ctcggaggcg agcgcggcaa
     1381 ggttcccacc ccattcgttg gcctcgagca ggatctcgtt cgacagaacg tcgagcgtct
     1441 tctgggcctc gccctggatg ttctgtgttc cggtaaggcc aaggccgtcg gccaacgctc
     1501 ccttgttgac gcgcgtgctg atcgccttgc aggcgcgcgc gatggtctcg atgagaagcc
     1561 tcaggtcggc gttgatgagg ttcttgtcgc gctgacggga aaccaggaac tgcgagagcg
     1621 tgacgcgctt catgcgctgg attttacttg ccttcgcgca aaccaggcgc cgcccagaag
     1681 gcacgcggcg agcagccaga acaccggcgc catgcccacg gcgctggagg cggcgccgga
     1741 ggcgagcggg atgagcgtat ggctgccgtt gaggagcgag gtgcgcacgc cgaccgcctc
     1801 gccctggcgc ccgggcgggg aggccgcgta cagcagcgac atgatgaccg gctgcgcgct
     1861 gccgagaccc aggccgagca cgaacgacag gaccatgagc ggcggcacgc tctgcgcgaa
     1921 cgggaagagc acgtagcagg tgcccgagat gccgagcgcg gcgaagatga tcacccattg
     1981 cctgagcttt ttgattagca cgggcatcgc gatgcgtacg gtgagcgtcg cgactgcgaa
     2041 ggtcgagagg actacgccga tcgcggcggc cgatagcccg aggcgcgcgc catagagcgg
     2101 aacgaggaaa gtgaagaggt cccagccggt ggcgagaagc aggctgaccc agaaagtctg
     2161 gcgcaggccg gggatgcgca gcagctcgag caccccggtg cgcctcgcga gcggcccctg
     2221 ctccgggcgc ggcaaagaac gttttcttcg caaaaccagg acgaaggcga cggcaggaaa
     2281 aaaggccaga atcaggaagg cgggcgcatg gccgaagccg tcgatggcgt agccggcgac
     2341 cagcggaccg atggagttgg agatcgcaaa cccgagcgcg agccatgaga aatttaccgc
     2401 acgctgctcc ggcgagccgt gcgcgccgaa aacgctgttc atggcgatgt gcacgtacat
     2461 gaacgaaacg ccgagcagcg tggtggacac gtacagcgtt tcgagcctcg gaaaggcgaa
     2521 gggcagggcg gccgaaatcg cgagcactgt gaaagaaagc gcaagtggcc ggcgcggccc
     2581 cacgcggtcg atgagacgtc cggcgctgac cgaaagcagc atcggcagca gcgcaaagag
     2641 cgacatcagc acgccgaccg cggcctcgga ggcgccaagc tcgagc
//
LOCUS       DASUNT010000015         2584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_703786, whole genome shotgun sequence.
ACCESSION   DASUNT010000015 DASUNT010000000
VERSION     DASUNT010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703786"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VFX94_00275"
     CDS             complement(<1..465)
                     /locus_tag="VFX94_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX5766640.1"
                     /translation="MKVDSKFTDFKVADLAAADWGRKEIAIAESEMPALMAIRKEFAA
                     AQPLRGARIAGSLHMTIQTAVLIETLKALGAEVRWASCNIFSTQDHAAAAIAASGTPV
                     FAYKGESLDEYWEFTHRIFEFGANKGPNMILDDGGDATLLVHLGRKAEQDPSV"
     regulatory      complement(507..590)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            640..1332
                     /locus_tag="VFX94_00280"
     CDS             640..1332
                     /locus_tag="VFX94_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766641.1"
                     /translation="MAAALRASLYASVVFLKIQDFARRPATEQARLRAQLQAVIAVTA
                     AEIAPAGRVMLDAADGAAIVVLDDPRAALRLAERALAAVTVGLPLSAGVNYGALELAG
                     RKRGEGMTGDGIAVAAAVAAYAPQARVLVSRAFRDAVADAAPGTEAVLAPAGTFTDPG
                     LRTHEVFGPTERAPGRRSRRYSMIAVMLAVALVATGVAVRSSTPDQRPFVDAVLERTG
                     PYWRNLVQRVSY"
     gene            1332..2459
                     /locus_tag="VFX94_00285"
     CDS             1332..2459
                     /locus_tag="VFX94_00285"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5766642.1"
                     /translation="MAKAAKKIGRYKILGELGRGAMGVVYRAEDPNLDRIVALKTISL
                     EKAEGRAEYQKRFMLEAKAAGKLSHPNIVTVYDFGEAEGLAYMAMELIEGTDLRKRAQ
                     QGAIPPVEAVEIASQVAEGLAFAHQRGIVHRDIKPANIMLPERGPAKITDFGLARMRL
                     ADHKTSTGIVLGTPRYMSPEQISGQPVDHRSDVFSLGIVLWEMLTGRRLFSGTEMAQV
                     SHSITYDEHEPPTRVNPELPAMLDFVVARALKKDPAVRYQDADEMAADLHTCLAELRA
                     AADLPDEATEASRTQKLPVDGEPTVGAPPAAAIAMDTRLPVSHHFDCTAALRRLSKAQ
                     QSGHPPRPVGILRRIITDAPARRIFVFTLLGALAGSYIALV"
BASE COUNT          426 a          887 c          864 g          407 t
ORIGIN      
        1 caccgaagga tcctgctcgg ccttgcggcc caggtgcacg agcagcgtcg catcaccgcc
       61 gtcgtcaagg atcatgttcg ggcccttgtt cgccccgaac tcgaagatcc tgtgcgtgaa
      121 ctcccagtac tcgtcgagcg attcgccctt gtaggcgaac accggcgtgc cgctcgccgc
      181 gatcgcggcc gccgcgtggt cctgggtcga gaagatgttg cacgatgccc agcgcacctc
      241 ggccccgagc gccttcagcg tctcgatcag caccgcggtc tggatcgtca tgtggagcga
      301 cccggcgatg cgcgcgccgc gcagcggctg cgcggcggcg aactccttgc ggatcgccat
      361 gagcgccggc atctccgact ccgcgatcgc gatttccttt cgcccccagt cggcagccgc
      421 gaggtcggcg accttgaaat cggtgaactt cgaatcgacc ttcatgagct caacgcctcc
      481 cgaaaacaaa aagcgcccgc accgtttcga tgcgagcgcc gttgaatggg tgacacgctt
      541 cgctgagcct gatccccgcg tacgcggaga acgcaacgct cctcagcgga tcgaattata
      601 ccctattcaa gcgcgctcaa tccctgcatc cttccccgca tggcagcggc cctgcgggcg
      661 tccctttatg cgagcgtcgt attcctgaag atccaggatt tcgcgcggcg tcccgccacc
      721 gaacaggccc gtttgcgcgc gcaactgcag gcggtgatcg cggtcacggc ggccgagatt
      781 gcgcccgccg ggcgcgtcat gctggatgcg gccgacggcg cagccatcgt cgtgctcgac
      841 gacccgcgcg cggcgttgcg gctcgcggag cgcgcgctgg ctgcggtgac ggtcggactc
      901 ccgctttccg ccggagtgaa ctacggggct ctcgagcttg ccgggcgcaa gcgcggcgaa
      961 ggcatgaccg gcgacggcat tgcggtcgcc gcggcggttg ccgcatacgc gcctcaggct
     1021 cgggttctgg tttcgcgcgc gttccgcgac gccgttgccg atgcggcccc gggcacagag
     1081 gcggtgctcg cgcccgcagg gacgttcacc gatccggggc tgcgcacgca cgaagtgttc
     1141 ggccccaccg agcgcgcgcc cggccgccgc agccgccgct acagcatgat tgccgtaatg
     1201 ctcgcggttg ctctggtggc caccggcgtt gcggtacgct cctccacccc ggatcagcgg
     1261 cctttcgtgg acgcggtgct cgaaagaacc gggccgtact ggcgcaacct ggtgcagcgg
     1321 gtgagctact gatggcgaaa gcggcgaaga agatcggccg ctacaagatt ctcggcgaac
     1381 tgggccgcgg cgcgatgggg gtggtctaca gggccgaaga cccgaacctc gatcgcattg
     1441 tggcgctgaa gaccatcagc ctggagaagg ccgagggccg tgcggaatac cagaagcgct
     1501 tcatgctcga ggccaaagcg gcgggaaagc tgagccatcc caacatcgtc acggtgtacg
     1561 actttggcga agccgaagga ttggcctaca tggcgatgga gctcatcgag ggaaccgatc
     1621 tgcgcaaacg cgcgcagcag ggcgccattc ctccggtcga agcggtggag attgcgagcc
     1681 aggtcgccga gggcctcgcg ttcgcacacc agcgcggcat cgtgcatcgc gacatcaagc
     1741 cggcaaacat catgctgccg gagcgcgggc cggcgaagat caccgatttc ggcctcgcgc
     1801 gcatgcgcct cgccgaccac aagacgagca ccggcatcgt gctcggcacg ccgcgctaca
     1861 tgtcgccgga gcagatttcc gggcagccgg tcgaccaccg ctcggatgtg ttctcgctcg
     1921 gcatcgtgct gtgggagatg ctcaccgggc ggcggctctt cagtggcacc gagatggcgc
     1981 aggtgagcca cagcatcacg tacgacgagc acgagccgcc gacgcgcgtg aatccggagc
     2041 tgcccgcgat gctcgacttc gtggtggcgc gcgcgctgaa gaaggacccc gcggtgcgtt
     2101 accaagacgc ggacgagatg gcggccgacc tgcacacctg tctcgcggag ctgcgcgcag
     2161 ccgcggacct gccggacgag gcgaccgagg cgagcaggac gcagaagctc ccggtggacg
     2221 gcgagccgac cgtcggagct ccgccggcgg ccgccatcgc catggacacg cgcctgcccg
     2281 tatcgcacca cttcgactgc accgcggcgt tgcggcggct ctccaaggcg cagcagagcg
     2341 ggcacccgcc gcgcccggtc ggaattctgc gcagaatcat caccgatgcg ccggcgcggc
     2401 gcatcttcgt cttcacgctg ctcggcgcgc tagccggcag ctacatcgcg ctcgtctagt
     2461 cctcgaaggt aatcagtccc atgccggttg ccatgggcgc gtagtaatgc cggtgcacgc
     2521 gtcttgcttt cggggaaagc gcgccgcgca tcacgtgcca catgatgagc tcgaccgcct
     2581 cggc
//
LOCUS       DASUNT010000016         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_90183, whole genome shotgun sequence.
ACCESSION   DASUNT010000016 DASUNT010000000
VERSION     DASUNT010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90183"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VFX94_00290"
     CDS             <1..506
                     /locus_tag="VFX94_00290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5766643.1"
                     /translation="REHGAREALDWQGRSTFAYGGATALAVGATTFITGAVGAARIAG
                     GWLVDRFPIPRVAIGSQLFALCGALIFSLFPGPRVAVPALAMIGMGYGVISGLTAAAI
                     ARYWGKNAFGRVAGRLYVAWCVAAISLPILAGWLYDRTGGYGAAVLIAGGLNLLAVGV
                     AARLPKP"
     gene            555..806
                     /gene="rpsP"
                     /locus_tag="VFX94_00295"
     CDS             555..806
                     /gene="rpsP"
                     /locus_tag="VFX94_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX5766644.1"
                     /translation="MVVIRLQRGGANKRPFFHVVVADSRRAAGKKFIERLGFYDPKAP
                     QGREALRVNMERVKHWTGKGAQLSPTAARLVKQFGKKPA"
     gene            820..1281
                     /gene="rimM"
                     /locus_tag="VFX94_00300"
     CDS             820..1281
                     /gene="rimM"
                     /locus_tag="VFX94_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX5766645.1"
                     /translation="MGRVAGSYGVRGWIRVEEAQAALARCAAWRIDGREFSVEQVKQH
                     SGTLLAKLRGVESPEAARKLKGSTVYVRREAMPAPEAGHYYLTDLVGLEVVNEQGVLL
                     GKVKRWMFNGAQDVMEVAGDGRVRLLPWIPGVVNNVDLQKRQIDVVWGADW"
     gene            1281..2021
                     /gene="trmD"
                     /locus_tag="VFX94_00305"
     CDS             1281..2021
                     /gene="trmD"
                     /locus_tag="VFX94_00305"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX5766646.1"
                     /translation="MRFDVVTLFPEMFAAITGSGITSRALEAGLYSLTTWNPRHFAPD
                     RYRTVDDRPYGGGPGMVMLAEPLEKTLDAVKAADGGRVVYLTPQGRKLDHRRVMELKD
                     ERALTLLCGRYEGVDERLLERRVDEEISIGDFVLSGGEVAAMALIDAVVRQLPGALGD
                     EASALEESFADGLLDCPQYTRPEVHAGLRVPQVLLSGHHGEIRRWRLKQALGRTWLRR
                     PDLLSARQLRAEEQGLLDEFKQETGSRG"
     gene            2052..2447
                     /gene="rplS"
                     /locus_tag="VFX94_00310"
     CDS             2052..2447
                     /gene="rplS"
                     /locus_tag="VFX94_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX5766647.1"
                     /translation="MDLIRTLEQEEIKRLKKEVPDFSPGDTVVVNVNVVEGERKRLQA
                     FEGMVIAKRNRGLNSSFIVRKVSSGEGVERTFQTYSPLIASIEVKRKGEVRRAKLYYL
                     RSRSGKSARIRERTGGEEGGGAGQGKAQE"
BASE COUNT          452 a          732 c          946 g          424 t
ORIGIN      
        1 cgcgtgaaca tggagcgcgt gaagcattgg actggcaagg gcgctcaact ttcgcctacg
       61 gcggcgcaac ggcgctcgcg gtcggcgcga cgacgttcat caccggcgcc gtcggcgcgg
      121 ctcgcattgc cggcggctgg ctcgtggacc ggttcccgat tccgcgcgtc gcgatcgggt
      181 cgcaactctt cgcgctgtgc ggggcgttaa tcttcagcct gttcccgggc ccgcgcgtcg
      241 ccgtacctgc gctcgcgatg atcggcatgg gctacggcgt catttccggg ctcactgccg
      301 cggcgattgc acgctactgg ggcaagaacg cgttcggccg tgtggcaggc cgactctatg
      361 tcgcctggtg cgtcgccgct atcagcctgc ccatactcgc cggctggctc tatgaccgga
      421 ccgggggcta cggcgcggcg gtgctcattg cgggcgggct gaatctactt gcggtcggcg
      481 tcgccgcacg cctaccaaag ccctgatttc tcccgtataa tccgcgcctt ttcgcggttt
      541 gcggagtatt cccgatggtg gtgattcgac tgcagcgcgg cggcgcgaac aagcggccgt
      601 tcttccatgt cgtggtggcc gactcgcggc gcgcggcggg caagaagttc atcgagcggc
      661 tcgggttcta cgacccgaaa gcgccgcaag gccgcgaggc gctgcgcgtg aacatggagc
      721 gcgtgaagca ttggactggc aagggcgctc aactttcgcc tacggcggcg cgcctcgtga
      781 agcagtttgg caagaaaccc gcctgaaaag cttgtagaga tgggccgcgt cgccggctcg
      841 tacggcgtgc ggggatggat cagggtggaa gaggcgcaag cggcgcttgc gcggtgtgcg
      901 gcatggcgga tagacggcag ggagttttcc gtcgagcagg taaagcagca ctcagggacg
      961 ttgctcgcga agctgagagg cgttgaaagt cccgaggcgg cccggaagct gaaggggtcg
     1021 acggtgtatg tacggcgcga ggccatgccc gcgcctgaag caggccacta ctacctcacg
     1081 gatctcgtgg gtttggaagt ggtgaacgag cagggagtcc tgctcgggaa ggtgaagcgc
     1141 tggatgttca acggcgcgca ggacgtgatg gaggtggcgg gggacggcag agtgcggttg
     1201 ctgccgtgga ttcctggagt cgtgaacaac gtcgacttgc agaaacgcca aatcgatgtg
     1261 gtgtggggag cggactggtg atccgcttcg acgtggtgac gctgtttccg gagatgttcg
     1321 ccgcgattac gggcagcggg atcacgagcc gggcgctgga agcgggcttg tattcgctga
     1381 caacgtggaa tccgcgccac ttcgcaccgg acaggtaccg gacggtcgac gaccggccgt
     1441 acggtggcgg gccgggcatg gtaatgctgg ccgagccgtt ggagaagacg ttggacgcgg
     1501 tgaaggccgc ggacggcggc agggtggtct acctgacgcc gcaaggaaga aagctcgatc
     1561 accgaagggt gatggagctg aaggacgagc gagcgctgac gctgctctgc ggccgctacg
     1621 aaggcgtgga tgagcggctg ctcgagcgcc gggtcgacga ggagatctcc atcggcgact
     1681 tcgtgctctc cggcggagag gtggcggcga tggcgctgat cgacgccgtg gtgcggcaac
     1741 tgccgggcgc attgggggac gaggcttctg cgctcgaaga gtccttcgcc gacgggctgc
     1801 tcgattgccc gcagtacacg cggcccgagg tgcacgcagg gctacgcgtg cctcaggtgc
     1861 tgctctcggg gcaccacggc gagatccggc gctggcggct gaagcaggcg ctgggcagga
     1921 cctggctgcg caggcccgat cttctctcgg cgcggcagct gcgcgcggaa gagcagggat
     1981 tattggacga gttcaagcag gaaacgggaa gcaggggata acctcccccg cccccctagg
     2041 agaggttgac catggatctg atccggacac tcgagcagga agaaatcaag cgcctcaaga
     2101 aggaagtgcc cgacttctcg ccaggcgata cggtggtggt gaacgtaaac gtcgtcgaag
     2161 gcgagaggaa gcgcctgcag gccttcgagg gcatggtgat cgcgaagcgc aatcgcggcc
     2221 tgaactcctc gtttatcgtg cgcaaggtgt cctcgggcga aggcgtggaa cgcaccttcc
     2281 aaacctattc gcccctgatc gcctcgatcg aggtcaagcg caagggggaa gtgcggcgcg
     2341 ccaagctcta ctacctgcgt tcgcgctcgg gcaagtcggc tcgcatccgg gagcggacgg
     2401 gcggagaaga aggcggcggc gcaggacaag gcaaagcgca ggagtaaaat gggctgatgc
     2461 gccgggaagc ggcagccatc agccctgtca gcgaagtcgt agcggcgatc cgcgccggcg
     2521 agatcgtcat cctggtcgac gacgaggacc gcga
//
LOCUS       DASUNT010000017         3933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_704073, whole genome shotgun sequence.
ACCESSION   DASUNT010000017 DASUNT010000000
VERSION     DASUNT010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704073"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1995)
                     /gene="ppsA"
                     /locus_tag="VFX94_00315"
     CDS             complement(<1..1995)
                     /gene="ppsA"
                     /locus_tag="VFX94_00315"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEX5766648.1"
                     /translation="MRVAWLKDLRLKDLAQVGGKNASLGEMIGGLAAAGIRVPGGFAT
                     TAEAFREFLGDLGPRIDERLRGLDLTDVDALASCGAEVRSWIVKQPFPAVLEQEIRSK
                     YQELTALTSSDTSFAVRSSATAEDLPEASFAGQQETFLNIRGVDNVLEAIRHVYASLY
                     NDRAISYRAHHGFEHSQVALSAAVQQMVRSDLGSAGVMFTIDTESGFQDVVFITSSYG
                     LGELLVQGAVNPDEFYVHKPMLEKGRPAIVRRGLGSKLQKMVFGRSDKAGKSTRVVDV
                     DEAQRSAFSLDDAGILELARYAVAIERHYGRPMDIEWAKDGADGRIYVLQARPETVKS
                     RTRDVEERFALKGSSRVLISGRAIGHKIGSGMVRIIPDASQMSGVKKGDVLVTDMTDP
                     NWEPVMKLAAAIVTNRGGRTCHAAIIARELGIPAVVGCGDATARLKDGQPVTVSCADG
                     DTGNVLEGLLPFEVSTRERGEMPRVPVKIAMNVGNPQRAFEFAQLPNAGVGLARLEFI
                     INNEIGVHPRACLEHEKLPAELKAKLLQKTRGYADPRTFYREKLVEGVATIAAAFWPK
                     PVIVRLSDFKSNEYKKLLGGERYEPDEENPMLGWRGASRYIAPDFRECFELECAALRK
                     VREELGFTNVEVMVPFVRTVGEAKAVVALLADNGLRRGQKD"
     gene            2017..2838
                     /locus_tag="VFX94_00320"
     CDS             2017..2838
                     /locus_tag="VFX94_00320"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEX5766649.1"
                     /translation="MAQRRTVFFVSDGTGITAQMLGHSLLTQFEGVEFNQVTLPFVDS
                     AEKAEECLARISAEADNNGQPIVFSTLVNNDVRAVVRRANALFIDFFESFIDPLEAGL
                     GIKSSHTIGRSHSAMDKKEYQQRIEAINFAMAHDDGASHRELGLADVILIGVSRSGKT
                     PTSLYLAMQFGVKAANYPLIPEDFHRGKLPEALRGDKSRLYGLTIAPERLHEIRKERR
                     PGSRYAELDNCRFEVDEAENLMRREGIRSINSTSKSIEEIATTILRELRIQRQIY"
     gene            complement(2813..3589)
                     /locus_tag="VFX94_00325"
     CDS             complement(2813..3589)
                     /locus_tag="VFX94_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX5766650.1"
                     /translation="MSVLTSPGNPKVRRWAKLAADACYRRSEKRALIEGPHLLAAALE
                     HGCRPVALLATEEGAADAEIGALIRESGVRPVLLAKRVFRRVVDAETPQGVAAEISIP
                     DSPARDGPVSVYLENIQDPGNVGAIVRTAAAFGIARIVFDRGCADAWSPKALRAGMGG
                     HFAVALKTAADLSAELNDFNGRLLSTVPRGGMPIEEADLTGPIGWLFGGEGRGLSEET
                     LRKVPLRVSIPMAEGSESLNVAAAAAICLHETFSRSAAGS"
     gene            complement(3586..>3933)
                     /locus_tag="VFX94_00330"
     CDS             complement(3586..>3933)
                     /locus_tag="VFX94_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX5766651.1"
                     /translation="IPDEEPVRHVSWHQANAYCAFAGRRLPSEAEWEYAALQGLENIG
                     HVWEWTSSAFLPYPGFVRDPYKEYSEPWFATHKVLRGASFATPPGVAHERFRNFYTPD
                     REDIFAGFRTCAL"
BASE COUNT          672 a         1336 c         1260 g          665 t
ORIGIN      
        1 gtctttctgg ccgcgccgca gcccgttgtc ggcgaggagc gcgacgaccg ccttcgcttc
       61 gccgaccgtc cgcacgaacg gcaccatgac ctcgacgttg gtgaagccga gctcctcgcg
      121 caccttgcgc agcgccgcgc attcgagctc gaagcattcc cggaagtcgg gcgcgatgta
      181 gcgcgaggcg cctcgccagc cgagcatcgg gttctcctcg tcgggctcgt agcgctcgcc
      241 gcccaggagc ttcttgtact cgttcgactt gaaatccgac aggcgcacga tcaccggctt
      301 cggccagaac gcggccgcga tcgtggccac gccctcgacg agcttctcgc ggtaaaaggt
      361 gcgcggatcg gcatagcccc tggttttttg cagcagcttc gccttcagct cggcgggaag
      421 cttctcgtgc tcgaggcagg cacgcggatg cacgccgatc tcgttgttga tgatgaattc
      481 cagccgcgcg agcccgacgc cggcgttagg aagctgggcg aactcgaacg cgcgctgcgg
      541 gttgccgacg ttcatcgcga tcttgaccgg gacgcgcggc atctcgccgc gttcgcgcgt
      601 cgagacctcg aagggcagga gcccctcgag cacgttgccg gtgtcgccgt cggcgcacga
      661 tacggtgacc ggttgcccgt ccttgagccg cgcggtggcg tcgccgcagc ccacgaccgc
      721 cgggatgccg agctcccgcg cgatgatcgc ggcgtggcag gtccggccgc cgcggttggt
      781 gacgatcgcg gcggcgagct tcatgaccgg ctcccagttg gggtcggtca tgtcggtgac
      841 gagcacgtcg ccctttttca ctccgctcat ctgcgacgcg tccgggatga tgcgcaccat
      901 gccgctgccg atcttgtggc cgatcgcgcg gccgcttatt agcacgcgag agctcccctt
      961 gagggcgaag cgctcctcga cgtcccgggt gcgcgacttg accgtctcgg gacgcgcctg
     1021 caggacgtag atgcggccgt cggcaccgtc cttcgcccat tcgatgtcca tcggccggcc
     1081 gtagtgcctc tcgatggcta ccgcgtagcg cgcgagctcc agaattccgg cgtcgtcgag
     1141 agaaaaagcg ctgcgctgcg cctcgtcgac gtccaccacc cgggtcgact tgcccgcctt
     1201 gtctgagcgc ccgaacacca tcttctgcag cttcgatccc aggccgcggc ggacgatggc
     1261 ggggcggccc ttctccagca tcggcttgtg gacgtagaac tcgtccgggt tgacggctcc
     1321 ctgaaccaga agctcgccca gcccgtagga cgaggtgatg aagaccacgt cctggaagcc
     1381 ggactccgta tcgatggtga acatgacgcc ggccgagccg aggtcgctgc gcaccatctg
     1441 ctggacagcc gccgacagcg cgacctggga atgctcgaag ccgtggtgag cccgatacga
     1501 gatggcccgg tcgttgtaga gcgaggcgta gacatgccgg atcgcttcga gaacgttgtc
     1561 cacgccgcgg atgttgagga acgtctcctg ctgtccggca aacgacgcct cgggcaggtc
     1621 ttcggccgtc gccgaggagc gcacggcgaa cgacgtatcg cttgaagtga gcgccgtaag
     1681 ctcctgatac ttggagcgta tctcttgctc gagaacagcc gggaatggct gcttgacgat
     1741 ccaggagcgg acctcggcgc cgcaactcgc caaggcatcg acgtctgtca ggtcgaggcc
     1801 tcgcaggcgc tcgtcgatgc gcgggccaag atctcctagg aactcgcgga aagcctcagc
     1861 ggtcgtggcg aagccgccgg ggacgcggat gccggcagcg gcgaggccgc cgatcatttc
     1921 gcctagcgag gcgttctttc caccgacctg ggccagatcc ttaaggcgga gatctttaag
     1981 ccacgctaca cgcattcgtt tggtatttta ggcgctatgg cgcagcgccg caccgtgttt
     2041 ttcgtctccg acggtacggg catcacggcg cagatgctcg gccacagcct gctcacccaa
     2101 ttcgaaggcg tggagttcaa ccaggtgacc ctgcccttcg tcgattcggc ggagaaggcc
     2161 gaggagtgcc tcgcgcgcat cagcgccgaa gcggacaaca acggccagcc gatcgtgttc
     2221 tcgacgctcg tcaacaacga cgtgcgcgcc gtggtgcgcc gggccaatgc cctgttcatc
     2281 gattttttcg agagcttcat cgatccgctg gaagcgggac tcggcatcaa atcgagccac
     2341 acgattgggc gctcgcacag cgcgatggac aagaaggaat accagcagcg catcgaggcg
     2401 atcaatttcg cgatggcaca cgacgacggc gcctcgcacc gcgagctcgg cctggccgac
     2461 gtgatcctga tcggggtgtc gcgcagcggc aagacgccga ccagcctata cctcgccatg
     2521 caattcggcg taaaggccgc gaattacccc ttgatccccg aagacttcca ccgcggcaag
     2581 ctgcccgagg cgctgcgcgg cgataaaagc cgcctttacg gcctcaccat cgcccccgag
     2641 cggctgcacg agatccgcaa ggagcgccgc cccgggagcc gctacgccga gctcgacaac
     2701 tgccgcttcg aggtcgacga ggcggagaac ctgatgcgcc gcgaaggcat ccgctcaatc
     2761 aactccacca gcaagtcgat cgaggagatc gccaccacga tcctgcgcga gctcaggatc
     2821 cagcggcaga tctactgaaa gtttcgtgga gacagatggc ggcggcggca gccacgttca
     2881 gcgactcgct gccttcggcc attggaatgc tgacgcgcag cggcaccttg cgcagcgtct
     2941 cctcgctcaa gccgcgtcct tcgccgccaa aaagccagcc tatcgggccg gtgagatccg
     3001 cctcttctat cggcatgccg ccacgtggaa ccgtggagag gagcctcccg ttgaagtcgt
     3061 tcagctccgc gctcaggtcg gcagccgtct tgagcgccac ggcgaaatgg ccgcccatgc
     3121 ccgcgcgcag cgccttgggc gaccaggcat ccgcgcagcc gcgatcaaaa acgatcctcg
     3181 caatcccgaa tgcggcggcg gttcgcacta ttgccccgac gttgcccggg tcctgtatgt
     3241 tctccaggta aacgctgacc gggccatcgc gagcgggtga atcggggatg gagatctccg
     3301 ccgccacacc ctgcggtgtt tcggcatcga ctacgcggcg aaaaaccctc ttcgccagca
     3361 gcaccggccg cacaccgctt tcccgaatca gcgcgccgat ttcggcatcc gctgcgccct
     3421 cctcggtcgc aagcagcgcg accggcctgc agccgtgctc gagcgcggcc gcgagcaggt
     3481 gcgggccctc gatcagcgcg cgcttctcgc ttcgccggta gcacgcatcc gcggcgagct
     3541 tcgcccagcg ccggactttg ggattgccgg gcgacgtgag cacgctcaca gcgcgcaggt
     3601 ccgaaagccg gcgaagatgt cctcccggtc gggagtataa aagttgcgaa agcgctcatg
     3661 cgccacgccg ggcggcgtgg cgaagctcgc gccgcgcagc accttgtgcg tggcgaacca
     3721 gggctcggaa tattccttgt acgggtcgcg cacgaaaccc gggtacggca ggaaagcgct
     3781 ggatgtccat tcccacacat ggccgatgtt ctcgaggccc tggagcgccg cgtactccca
     3841 ttccgcttcg ctcggcaacc gccggccggc gaacgcacaa taagcgttcg cctggtgcca
     3901 gctcacgtgg cgcaccggct cttcgtcggg aat
//
LOCUS       DASUNT010000018         2667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_704216, whole genome shotgun sequence.
ACCESSION   DASUNT010000018 DASUNT010000000
VERSION     DASUNT010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704216"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..1533)
                     /locus_tag="VFX94_00335"
     CDS             complement(193..1533)
                     /locus_tag="VFX94_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5766652.1"
                     /translation="MADALAAHAAKRESLRESLRQGAGPVALAKETSNLFRDREAGRR
                     RRLDVRGFNQVLRIAPGWVEAEGMIAYEELTRECLAHGVMPAVVPQLKTITLGGAVAG
                     VGIESSSYRYGLVHDTMLELDVLLGDGRVVACTPDNAHADLFFGFPNSYGTLGYALRV
                     KAKTVAVKPYVRLRHLAFSDAGEYFADLERHLEERKADFIDGTIFSPERLFLTLGELV
                     DSAPYTSDYTFEKIYYQSIGEKREDYLAVHDYIWRWDTDWFWCSKNVFAQNPLMRRFV
                     YGRDRLGSRTYTKIMRWNSRVGATKKLERVLGLHSESVIQDVDIPISRAAEFLDFYAR
                     EIALWPQWVCPIGPQANCGRFVLYPVKNAWYVNFGFWDVKRTRGAHPPGHFNRLIEEK
                     VSALGGIKSLYSESFFTEADFRARYGGAAYDALKRKYDPTAVFPRLYEKCVLRA"
     gene            complement(1526..>2667)
                     /locus_tag="VFX94_00340"
     CDS             complement(1526..>2667)
                     /locus_tag="VFX94_00340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX5766653.1"
                     /translation="VFRSPRAYRRIAAFGHVGLLEAYFAGELDIEGSLARALAAGMEG
                     GIDGRVGLLVWLRNRWHEFTHSNAVRSTARANAEAHYALPPEFYQFWLDDPLMLYTCA
                     YWTEGTRTLEEAQRNKCDHVARKLLLKPGEEVIDVGSGFGGFMFHAHERYGVKVTAVN
                     TTGSQADHARREIERKRLGDSLSMQMHEDFRDAGQRQFDKVASIGCLEHAGRDQLREL
                     IHAHAGYLKKGGIGLIHFIGHVGRFDTDFFIRKYVFPGGWIPSLADVIVEMEKAGLEV
                     LDVENLRRHYALTLDVWADRFDRNWEKIRALDPKRFDERFRRIWRVYLYGCAELFRSP
                     AGRTHLFQVVFSKGNVTRSSYPMTRDFLYEQVEAEAAAPRRVGHG"
BASE COUNT          455 a          866 c          898 g          448 t
ORIGIN      
        1 ggaatgcggc ggcgacatcg gcttcgatcg cctgcgtgcg aatctgtcgg cgctgcgctg
       61 cgtggactgc cagcgcgtgc acgagaagac cttcgccggg ccgagcgcct cgagcctttg
      121 aggcaatgcg gggcaattcc gggacgcccg aactagttag ggctccgtcc cggaattgcc
      181 tccccgcact gctcaagccc gtagaacgca tttctcgtaa agccggggga agacggccgt
      241 cgggtcgtac ttgcgcttca gcgcgtcgta ggcggcgccg ccgtagcgcg cgcggaagtc
      301 cgcctcggtg aagaagctct cggagtagag cgacttgatg ccgcccagcg cgctcacttt
      361 ttcctcgatc agacggttga agtgcccggg cgggtgggcc ccgcgcgtgc gcttcacgtc
      421 ccagaagccg aagttgacgt accaggcgtt tttcaccgga tagagcacga accgcccgca
      481 attcgcctgg gggccgatcg ggcagaccca ctgtggccag agcgcgatct cgcgcgcata
      541 gaaatcgagg aactcggcgg cgcgcgagat gggaatgtcg acgtcctgga tgaccgattc
      601 ggaatgcagg ccgagcacgc gctcgagctt tttcgtcgcg ccgacgcggc tattccagcg
      661 catgattttc gtgtaggtgc gcgagccgag gcgatccctc ccgtagacga agcgccgcat
      721 cagggggttc tgcgcgaaca cgttcttcga gcaccagaac cagtcggtgt cccagcgcca
      781 gatgtagtcg tgcaccgcca gatagtcctc gcgcttctcg ccgatcgact ggtagtagat
      841 tttctcgaag gtatagtcgc tcgtgtacgg cgccgaatcg acgagctcgc ccagggtgag
      901 gaagagccgc tcgggcgaga aaatggtgcc gtcgataaaa tcggccttgc gctcctcgag
      961 atgccgctcg agatcagcga agtactcgcc tgcgtccgaa aaagccaggt gcctcaggcg
     1021 cacatacggc ttcaccgcga cggtcttcgc tttcacgcgc agcgcgtagc cgagcgttcc
     1081 gtacgagttg gggaagccga agaaaaggtc ggcatgcgcg ttgtcgggcg tgcaggcgac
     1141 gacccgcccg tcgccgagca gcacgtcgag ctcgagcatc gtgtcgtgca ccagcccata
     1201 ccggtacgac gacgactcga tacccacgcc ggccactgcg ccgccgaggg tgatggtctt
     1261 cagctgcggg accaccgcgg gcatcacgcc gtgcgcgaga cactcgcgcg tgagctcttc
     1321 gtacgcaatc atgccttcgg cctcgaccca gcccggcgcg atgcgcagca cctggttgaa
     1381 gccgcggaca tcgagccgcc gccgcctgcc cgcctcgcgg tcgcggaaga ggttggaggt
     1441 ctccttcgcc agcgcgaccg gcccggcgcc ttgcctcaga gattcgcgga gagactcgcg
     1501 cttggcggcg tgcgccgcga gcgcgtcagc catgcccgac gcggcgcgga gcggcggctt
     1561 cggcttccac ctgctcgtac aggaagtcgc gggtcatcgg atagctcgag cgcgtgacgt
     1621 tgcccttgga gaacacgacc tggaagaggt gcgtgcggcc ggcgggcgag cggaacaact
     1681 cggcgcagcc gtagagatac acccgccaga tgcgccggaa gcgctcgtcg aagcgcttcg
     1741 ggtcgagcgc acggatcttt tcccagttcc tgtcgaagcg atcggcccac acgtccagcg
     1801 tgagcgcata gtggcggcgc aaattctcga cgtcgagcac ttcgaggccg gctttttcca
     1861 tctcgacgat cacgtcggca aggctcggga tccagccgcc ggggaacacg tacttgcgga
     1921 tgaagaaatc ggtgtcgaag cgcccgacgt ggccgatgaa gtggatgagc ccgatgccgc
     1981 ccttcttcag gtagccggca tgggcgtgga tgagctcgcg aagctggtcg cgcccggcgt
     2041 gctccaggca tccgatggaa gccaccttgt cgaactgtcg ctggccggcg tcgcggaagt
     2101 cctcgtgcat ctgcatggag agcgaatcgc cgaggcgctt gcgctcgatc tcgcggcgcg
     2161 catggtccgc ctgcgagccc gtggtgttga ccgcggtgac cttcacgcca tagcgctcgt
     2221 gcgcgtggaa catgaagccg ccgaagccgc tgcccacgtc gatcacctcc tcgcccggct
     2281 tgaggaggag cttgcgcgcc acgtggtcgc acttgttgcg ctgcgcttcc tcgagcgtgc
     2341 gcgtgccctc ggtccagtac gcgcacgtgt agagcatgag cggatcgtcg agccagaact
     2401 ggtagaactc cggcggcagc gcatagtgcg cctccgcgtt cgcgcgcgcc gtggagcgca
     2461 cggcgttcga atgggtgaat tcgtgccagc ggttgcgcag ccagacaaga aggcccacgc
     2521 ggccgtcgat gccgccttcc atgccggcgg caagcgctct cgcgaggctg ccctcgatgt
     2581 cgagctcgcc ggcaaagtac gcttccagaa gcccgacgtg gccgaaggcc gctatgcgcc
     2641 ggtaggcgcg cgggctgcgg aacacca
//
LOCUS       DASUNT010000019         3368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_178271, whole genome shotgun sequence.
ACCESSION   DASUNT010000019 DASUNT010000000
VERSION     DASUNT010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178271"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1678
                     /locus_tag="VFX94_00345"
     CDS             <1..1678
                     /locus_tag="VFX94_00345"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5766654.1"
                     /translation="CTAAAPAVSTWRVTGAQSLLRALRAMGVERIYASPGSDWAPVWE
                     ALAGAKPGELPQYISVRHEETAVAMANGYAKASGNLPAVLLHTTVGTLHAAMALRAAL
                     HERVPMAVLAGESAAFAEDGLPLGRQWLRLLTDIGGPARLIEPCVKSSFASSASELLA
                     STVQRACQLALSAPRGPVFLSVPIEHLMAEASAELPIASLPAAPAPDPAALDALAREL
                     DAARSPLIITEEAGRDRSAVDALVALAEALGAPVLEGWQPYYFNFPREHPLYGGVLVD
                     AVPECDAVLLVDAVLPWHPPSAVKERKVFVLGEDPLRSRLPYWGFRSDAIVPGDVRAS
                     VAGLAARLGRGSSKSNSSWRARRESERHALRAAARAEARMTNAWVAHELNELLPREAV
                     LVNETITHRLDLLRRVERLGPGGFYEASFGGLGVGLGVALGVKHAEPARTVVCTIGDG
                     AFHYNPVPASFGAAQEHGLPILVILFNNAGYLSQKTDVEKYYPAGQAVKSGRFAGTSI
                     APMPDYAALARAYGGAGERVEDPRELRAALQRGFDAVAQGRLALVDVVLG"
     gene            1675..2505
                     /locus_tag="VFX94_00350"
     CDS             1675..2505
                     /locus_tag="VFX94_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5766655.1"
                     /translation="MKSFVPPPLACDAHCHVFGPAAKFPYDPRAAYQPEDAPFEELQK
                     LHSRLGLQRAVIVHASCHGADMRVTLDAIARSGGSFRGTAIIDSSVSEKDFERMHEGG
                     IRAVRFNFVRHLGGRPSMDFFQRTVQKLQSFGWHLILHLDAQDLVELEELLSTLPVPF
                     VIDHMGRVKAGDGLEQRPFKTLLGFLKNENAWVKICGAERVSSKGPPFTDAVPFGRAL
                     VEAAPERVLWGTDWPHPNVRWMPDDGALLELFGLMVPEPALQQMILVNNPARLYGFGI
                     "
     gene            2537..3295
                     /locus_tag="VFX94_00355"
     CDS             2537..3295
                     /locus_tag="VFX94_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5766656.1"
                     /translation="MAEDIILETRGLTKEFKGFVAVKDVSLKVRRGTIHALIGPNGAG
                     KTTCFNLLTHFLEPTRGQIFFKGREITGSPPARIARMGLVRSFQISAVFPHLTVLENV
                     RIALQAKRGRSFDFWRSERMLERLNAAADELIDAVGLKEFSRTVAVELPYGRKRALEI
                     ATTLALDPEMMLLDEPTAGMTHEDVERIAALIKRVSANRTVLMVEHNLSVVSTLSDTI
                     TVLQRGEILAEGDYASVSKNPQVVEAYLGAGGHA"
BASE COUNT          519 a         1133 c         1148 g          568 t
ORIGIN      
        1 atgtacagcc gcggccccgg ctgtgtcaac atggcgcgtg accggggcgc aatctctgct
       61 gcgcgcgctg cgcgcgatgg gcgtggagcg catctacgcc tcacccggct ccgactgggc
      121 gccggtgtgg gaggcgctcg ccggcgcaaa gccgggcgag ctcccgcagt acatctcggt
      181 tcgccacgag gaaaccgcgg tggcgatggc gaacggctat gccaaggcga gcgggaacct
      241 gcccgccgtc ctgcttcaca ccacggtcgg aacgctgcat gcggcgatgg cgctgcgcgc
      301 tgcgctgcac gagcgcgtgc cgatggctgt gctcgctggc gaatcggcag cctttgccga
      361 ggacggcttg ccgctcgggc gccaatggct gcggcttctc accgacatcg gcggcccggc
      421 gcggctgatc gagccgtgcg tcaagtcgag ctttgcctcg agcgcctcgg aacttctcgc
      481 cagcacggtg caacgcgcct gccagctcgc gctgagcgcc ccgcgcggtc cggtgttcct
      541 ctcggtgccg atcgagcacc tgatggcaga agcatctgca gaactaccca tcgccagcct
      601 gcccgccgcg ccggcgccgg atcccgcggc gctcgatgcg ctcgcgcgcg agctggacgc
      661 cgcgcgcagc ccgctgatca tcaccgaaga agcgggacgg gatcgcagcg cggtggatgc
      721 gctcgtcgcg ctcgcggagg cgctcggcgc gccggtgctc gagggctggc agccgtatta
      781 cttcaacttc ccgcgcgagc atccgctcta tggcggcgtc ctggtcgatg ctgtcccgga
      841 atgcgacgcc gtgctcctcg tggatgccgt gctgccgtgg cacccgccct ccgcagtgaa
      901 ggagaggaag gttttcgtgc tgggcgaaga tccgctacgt tccaggcttc cttactgggg
      961 ctttcgcagc gacgcaatcg ttccgggcga tgtgcgggcg agcgtcgccg gcctcgcagc
     1021 ccggctgggc aggggttctt cgaaaagcaa ctcttcctgg cgggcgagac gcgaaagcga
     1081 gcgccacgcg ctccgcgccg ccgcgcgggc cgaggctcgc atgaccaacg cctgggtcgc
     1141 gcacgagctg aacgagctgc tgccgcgcga agcggtgctg gtcaacgaga cgatcacgca
     1201 ccggctcgat ctcctgcgcc gggtcgagcg cctcggcccg ggcgggttct acgaggcgag
     1261 cttcggcggc ctcggcgtcg ggctcggcgt tgcgctcggc gtgaagcacg ccgagcccgc
     1321 gcgcacggtg gtgtgcacga tcggcgacgg cgcgttccac tacaaccccg tgcccgcttc
     1381 cttcggtgcc gcgcaggagc acgggctccc gattctcgtg atcctcttca acaatgcggg
     1441 ctacctctcg caaaagaccg acgtcgagaa gtactaccca gcggggcagg ccgtgaagag
     1501 cggcaggttc gccggcactt cgattgctcc gatgcccgac tacgccgcgc tcgcgcgcgc
     1561 gtacggcggt gctggcgagc gcgtggaaga tccgcgggag ctgcgcgccg ccctgcagcg
     1621 cggcttcgac gcggtcgcgc aagggcgcct cgcactggtg gacgtggtcc tcggatgaaa
     1681 agcttcgtgc cgccgccgct tgcctgcgat gcacattgcc atgtgttcgg gccggcggcg
     1741 aagtttccct acgatcccag ggcggcctac cagcccgagg atgcgccgtt cgaggagctg
     1801 caaaagcttc attcccgcct gggtcttcag cgtgcagtca tcgtacatgc gagctgccac
     1861 ggggcagaca tgcgggtcac gctcgacgcg atcgcgcgct cgggcgggag ctttcgcgga
     1921 accgcgatca tcgattcttc ggtttccgaa aaggacttcg agcgcatgca cgagggcggc
     1981 atccgggcgg tccgcttcaa cttcgtgcga cacctcggcg gccggccgag catggatttt
     2041 tttcaacgga cggtccagaa actacagtcc ttcggctggc atctcatcct gcacttggac
     2101 gcgcaggacc tggtggagct ggaagagctc ttaagcacgc tgccggtccc gttcgtcatc
     2161 gaccacatgg ggcgggtgaa ggcgggcgac gggctcgagc agcggccttt caagacgcta
     2221 ttggggttct tgaagaatga gaatgcgtgg gtgaagatct gcggggccga gcgcgtctcc
     2281 tcgaaaggtc ctcccttcac cgacgcggtg cccttcggcc gggcgctggt cgaggctgcg
     2341 cccgagcgcg tgctgtgggg aacggactgg ccccatccga acgtcagatg gatgcccgac
     2401 gatggtgcgc tgctcgagct cttcggcctg atggtcccgg agccggcgct gcagcagatg
     2461 atcctcgtca acaaccccgc gaggctctac ggcttcggta tatgatcgcg cgcttcagca
     2521 ccacccaggg ttagagttgg ccgaggacat cattctcgaa acgcgcggct tgacgaagga
     2581 gttcaagggc ttcgtcgccg tcaaggacgt gtcactgaag gtgcgccgcg gcaccatcca
     2641 cgcgctgatc ggccccaatg gcgccggcaa gaccacctgc ttcaacctgc tcacgcactt
     2701 cctcgagccc acgcgcggac agattttttt caagggacgc gagatcaccg gctcgccgcc
     2761 cgcgcgcatc gcgcgcatgg gcctggtgcg ctcgttccag atctccgcgg tctttccgca
     2821 tctcacggtg ctcgagaacg tgcgcatcgc gctgcaggcg aagcgcggcc gctcgttcga
     2881 cttctggcgc tccgagcgca tgctcgagcg gctgaacgcc gcggccgacg agctgatcga
     2941 cgcggtcggc ctgaaggaat tctcgcgcac cgtcgcggtc gagctgccct acggccgcaa
     3001 gcgcgcgctc gagatcgcga ccacgctcgc gctcgatccc gagatgatgc tgctcgacga
     3061 gcctaccgcg ggcatgacgc atgaggatgt ggagcgcatc gctgcgctga tcaagcgcgt
     3121 gtcggcaaat cgcacggtcc tcatggtgga gcacaacctg tcggtggtgt cgacgctttc
     3181 cgacaccatc accgtgctcc agcgcggcga gatcctggcc gagggcgact acgcctccgt
     3241 ctcgaagaat ccgcaggtgg tggaggcgta ccttggagcc ggcggccatg cttgagatcc
     3301 gcggcctgca ctcctggtac ggagagtcgc acatcctgca cggaatggag ctcgacatcg
     3361 ccgagggc
//
LOCUS       DASUNT010000020         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_353813, whole genome shotgun sequence.
ACCESSION   DASUNT010000020 DASUNT010000000
VERSION     DASUNT010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353813"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VFX94_00360"
     CDS             <1..613
                     /locus_tag="VFX94_00360"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5766657.1"
                     /translation="VARVLSESVVLAVRTDAQWRTLNELIQEAKRKPKAISVGHSGIG
                     SHTHISLAALVNSAGIEVNEVPFAAAQVVPSLVGGHVNAVVQFPGALAAPLKQGQARL
                     LATLTQNRDPAWPDVPTARELGVDVVLDAWRGIAVPRGTPRQVVSQLESAIRMTASSP
                     DFIAQSEKLNVRPAFLPAEEFSALVAKEDAELSRLMQQIGLKK"
     gene            complement(543..>2141)
                     /locus_tag="VFX94_00365"
     CDS             complement(543..>2141)
                     /locus_tag="VFX94_00365"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX5766658.1"
                     /translation="HLERAQVSLEQDAQGRRNWILQQTREKEESRLFIRLLTLDDALV
                     RWEDAARKHSLAVDLSTDDSGVQFMAVGSYGGKPLKAHGHAGQVLSIRDPDTPFPLKG
                     DFSIGGTSARLEGTLTGIVDMKGIDLRLERLAGKSMDELYDIVGLAFPATSPYTVSGR
                     LSRTDGTWRFEDFAGKVGESDLGGWIRVQTGAGKRPLMQGEVKAKVLNFADLGALVGT
                     DRPRKNGVLPDMPFSRERWESVDADVKIEAGTIKRPAQLPIERFAARIVMHERVLTLD
                     PLAFGIAGGKLAGTVRLDGNHEPMRGEVRMRVQNLQLAKIFPRIEQTRSSVGDLDGVM
                     ELSGSGDSVGKLLASANGKVGVYIDQGRISQYLMELIALDLWDVARIKLRGDRDVEIR
                     CAIADFGVKNGVMQANALVFDTSVVNVGGSGSIDLKNERMDITLRPQPKDGSPASLRT
                     PLHMRGTFGQPEIGPDMRRIAARGAGMLAMGILNPLLTILPLLDEGKGKDSNCAGLIE
                     EATSSGRSAASGATAPRPPSPPRR"
BASE COUNT          329 a          746 c          704 g          362 t
ORIGIN      
        1 ggtcgcgcgc gtgctgtcgg aatcggtggt gctcgcggtc aggaccgacg cccagtggcg
       61 cacgctgaac gagctgatcc aggaagcgaa gcgcaagccc aaggcgattt ccgtcggaca
      121 ctcaggcatc ggcagccata cccacatttc gcttgcggcg ctcgtcaact ccgccgggat
      181 cgaagtgaac gaggtgccgt tcgccgcggc ccaggtggtg ccgagcctcg tcggcggcca
      241 cgtcaacgcc gtggtgcaat tcccgggcgc gctcgccgcg ccgctcaagc aggggcaagc
      301 ccgcctgctc gcgacgctga cgcagaaccg cgaccctgcg tggcctgacg tgccgacggc
      361 gcgcgagctc ggcgtcgacg tcgtgctgga cgcatggcgc ggcatcgcgg tgccgcgcgg
      421 cacgccgcgc caggtggtga gccagctcga atccgcgatc cggatgacgg ccagttcccc
      481 ggacttcatc gcgcagagcg agaagctcaa cgtgcggccg gcattcctcc cggccgagga
      541 gttcagcgcc ttggtggcga aggaggacgc ggagctgtcg cgcctgatgc agcagatcgg
      601 cctgaagaag tagcttcctc gatcagcccg gcgcagtttg agtccttgcc tttgccctcg
      661 tcgagcaggg gcaggatggt gagcagcgga ttgagaatcc ccatggcgag catgcccgcg
      721 ccacgcgccg cgatccggcg catgtccggg ccgatctccg gctgcccgaa cgtgccgcgc
      781 atgtgcagcg gcgtgcgcag cgaggccggg ctgccgtcct tgggctgcgg cctgagcgtg
      841 atgtccatgc gctcgttctt caggtcgatg cttccgctgc cgcccacgtt gaccaccgag
      901 gtgtcgaaga cgagcgcatt cgcctgcatc acgccgttct tcacgccgaa atcggcgatc
      961 gcgcagcgga tctcgacgtc cctgtcgccg cgcagcttga tgcgcgccac gtcccacagg
     1021 tccagcgcga tgagctccat caggtactgg ctgatccgcc cctggtcgat gtagacgccg
     1081 accttgccgt tcgccgaggc gaggagcttg ccgaccgagt cgccgctccc cgaaagctcc
     1141 atcacgccgt cgaggtcgcc caccgacgat cgcgtctgct cgatgcgggg gaatatcttc
     1201 gcgagctgca ggttctgcac gcgcatcctc acttcgccgc gcatcggctc gtggttgccg
     1261 tcgaggcgca ccgtgccggc gagcttgccg cccgcgatgc cgaaggcgag cgggtcgagc
     1321 gtgagcacgc gctcgtgcat cacgatgcgc gcggcgaacc gctcgatcgg cagctgcgcc
     1381 gggcgcttga tcgtgccggc ttcgatcttg acatccgcgt ccacgctctc ccagcgctcc
     1441 cgcgagaacg gcatgtcggg cagcacgccg ttcttgcgcg gccggtcggt gccgaccagg
     1501 gcaccgagat cggcgaaatt caggaccttc gcctttactt ctccttgcat gagcgggcgc
     1561 ttccccgccc cggtctgcac gcggatccac ccccccaggt cgctctcgcc gacctttccg
     1621 gcgaagtcct cgaagcgcca tgtaccgtcg gtccgggaga ggcggcccga cacggtgtag
     1681 gggctagtcg ccgggaaagc gagtccgacg atatcgtaga gctcgtccat cgacttgccg
     1741 gcgaggcgct ccaagcgcag gtcgatgcct ttcatgtcga cgatgccggt gagcgtgcct
     1801 tccaggcggg ccgaagtacc accgatgctg aagtcgccct tcaacggaaa cggcgtgtcc
     1861 gggtcgcgga tcgacagcac ctgccccgca tgcccgtgcg ccttgagcgg cttgccgcca
     1921 tacgagccca ccgccatgaa ctgcactccg ctgtcgtcgg tggaaagatc aacggcaagg
     1981 ctgtgctttc gggcggcgtc ctcccagcgc acgagcgcat cgtccagcgt caagagcctt
     2041 atgaaaagcc gcgactcttc cttctcccgc gtctgctgca ggatccagtt cctgcggcct
     2101 tgagcgtcct gctcaagcga tacctgggcg cgctcaaggt g
//
LOCUS       DASUNT010000021         2336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_266019, whole genome shotgun sequence.
ACCESSION   DASUNT010000021 DASUNT010000000
VERSION     DASUNT010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266019"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /gene="rsfS"
                     /locus_tag="VFX94_00370"
     CDS             <1..285
                     /gene="rsfS"
                     /locus_tag="VFX94_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX5766659.1"
                     /translation="IASGDSNRQVKALSERVQERVRELGARVYGVEGEAGGEWVLVDL
                     GDVVVHIMHPAVRTFYNLEEIWGEKPVQMKRAAPRKAKRRAKAPRRRRKR"
     gene            251..649
                     /locus_tag="VFX94_00375"
     CDS             251..649
                     /locus_tag="VFX94_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX5766660.1"
                     /translation="MPRLRVVAVSARLPGWAEAACADYLERMPRGYEVERSRARPGRL
                     ALPRNSRIVALDEHGKDMTTPQFASLLKTETTFVIGGPDGLDTQVKKDAALLLRLSSL
                     TLPHALAQVVLIEQIYRAATLLSGHPYHRA"
     gene            646..1272
                     /locus_tag="VFX94_00380"
     CDS             646..1272
                     /locus_tag="VFX94_00380"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX5766661.1"
                     /translation="MISIPERGIYLASRSPRRRELLSQIGVRYHLLLFRERLGERPEV
                     DETVLEGESAAAYVERMARAKAEAGWNRMLQRNLPQAPILAADTTVALGGRILGKPAD
                     RAEAAEMLKLLSGERHEVLTGVALKSHDFVEFSLSISEVLLKKLSPEEIAQYVASGEG
                     DDKAGAYAIQGRAARFIVELRGSYSGVMGLPLYETSLLLEKLRGRNSY"
     gene            1253..>2336
                     /locus_tag="VFX94_00385"
     CDS             1253..>2336
                     /locus_tag="VFX94_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX5766662.1"
                     /translation="MEEILINVTPQETRVAITAAGVVQELLIERAASRGLVGNIYVGR
                     VARVLPGMQSAFIEIGLERAAFLHVADIWDRRERPIEKILAEGEPVLAQVVKYPIGSK
                     GARLSTQVSIAGRLLVYLPHDPHIGISQRIEDEGGRAALRERLKELVPPDEKGGFIVR
                     TLAEAAAEEELRADLAYLRELWKAVAERSLGAEPPKLVYQDLSLAQRVLRDLVSAATI
                     GVLVDSRENFQKLAAFAERYMPQVRLRVEHYTGERPLFDLYNVETEIERALSRRVELK
                     SGGYLIIDQTEAMTTIDVNTGGFVGNRNFDDTVFKTNLEAAQAIARQLRLRNLGGIII
                     IDFIDMDSAEHRNAVLDELRRALARDR"
BASE COUNT          394 a          777 c          823 g          342 t
ORIGIN      
        1 atcgcgagcg gcgactcgaa ccgccaggtg aaggcgctct ccgagcgcgt gcaggagcgg
       61 gtgagggaac tcggcgcccg ggtctacggc gtcgaaggcg aggcgggggg cgagtgggtg
      121 ctggtcgacc tcggcgacgt agtggtgcac atcatgcatc ccgccgtgcg gaccttctac
      181 aacctggagg agatctgggg cgagaagccc gtgcagatga agcgggcagc gccccggaaa
      241 gcgaaacgcc gtgccaaggc tccgcgtcgt cgccgtaagc gctaggctgc cgggctgggc
      301 cgaggccgcg tgcgccgact accttgagcg catgccgcgc ggctacgagg tcgagcgctc
      361 gcgcgccagg cccgggcgcc tcgctttgcc gaggaattcg cgcatcgtgg cgctggacga
      421 acatggcaag gacatgacga cgccgcagtt tgcgagcctg ctgaaaaccg agacgacctt
      481 cgtcatcggc ggtcccgacg ggctggacac gcaggtgaaa aaggacgcgg ccctgctgct
      541 gcgcctttca tcccttaccc tgccgcatgc gctcgcccaa gtggtgctga tcgagcagat
      601 ttaccgcgcc gcgacgcttc tgagcgggca tccttaccat cgcgcatgat ctccattccc
      661 gagcgcggca tctacctcgc ttcgcgcagc ccgcggcggc gcgagctcct ttcgcagatc
      721 ggcgtgcgct accacctgct gcttttccgc gagcgcctgg gcgagcgccc tgaggtggac
      781 gaaaccgtgc tcgagggcga atctgcggcg gcgtacgtcg agcgcatggc gcgcgccaag
      841 gcggaggccg ggtggaaccg gatgctgcag cgaaacctgc cgcaggcgcc gatactcgcg
      901 gccgacacga ccgtggcgct cggcggccga atcctcggca agcccgcaga ccgcgccgag
      961 gcggccgaga tgctgaagct cctttccggc gagcgccacg aggtgctgac cggcgtggcg
     1021 ctcaagagcc acgacttcgt ggagttctcg ctttcgatct cggaagtgct gctgaagaag
     1081 ctctcgccgg aggagatcgc gcaatacgtc gcgagcggcg aaggcgacga caaggcgggc
     1141 gcctacgcga tccagggccg ggccgcgcgc ttcatcgtcg agctgcgcgg cagctactcg
     1201 ggggtgatgg ggctgccgct gtacgagacg agcctgctcc tcgagaagct gcgtggaaga
     1261 aattcttatt aacgtcacgc cgcaggagac gcgggtagcg atcaccgcgg ccggcgtggt
     1321 gcaggagctc ctcatcgagc gggcagcgag ccggggactc gtgggcaaca tctacgtcgg
     1381 gcgcgtggcg cgagtgctgc ccggtatgca gtccgcgttc atcgagatcg gcctcgagcg
     1441 cgccgccttc ctgcacgttg ccgacatctg ggaccggcgc gagcgcccga tcgagaagat
     1501 cctcgccgaa ggcgagcccg tgcttgcgca ggtggtgaaa tacccgatcg ggtcgaaagg
     1561 cgcgcggctc tccacgcagg tgtccatcgc ggggcggctg ctcgtctacc tgccgcacga
     1621 cccgcacatc ggcatttcac agcgcatcga ggacgagggc gggcgcgccg cgctgcgcga
     1681 gcggctgaaa gagctcgtgc cgcccgacga gaaaggcggc ttcatcgtgc gcacgctggc
     1741 cgaggcggcg gccgaggaag agctgcgcgc cgacctcgcg tacctgcgcg agctgtggaa
     1801 ggcggtggcc gagcgctcgc tcggcgccga gccgccgaag ctcgtctatc aggacctgtc
     1861 gctcgcgcag cgcgtgctgc gcgacctggt gagcgccgcg accatcgggg tgctggtcga
     1921 ctcgcgcgag aacttccaga agctcgccgc gttcgccgag cgctacatgc cgcaggtgcg
     1981 gctgcgcgtc gagcactaca ccggcgagcg gccgctgttc gacctctaca acgtggagac
     2041 cgaaatcgag cgcgcgctct cgcgccgcgt ggagctcaag tcgggcggtt acctcatcat
     2101 cgaccagacc gaggcgatga ccaccatcga cgtgaacacc ggcggcttcg tcggcaaccg
     2161 caacttcgac gacacggtgt tcaagaccaa cctcgaggca gcacaggcga tcgcgcggca
     2221 gctgcgcctg cgtaacctgg gcggcatcat catcatcgac ttcatcgaca tggacagcgc
     2281 cgagcatcgc aacgccgtgc tcgacgagct gcgccgggcg ctcgcacgcg accgca
//
LOCUS       DASUNT010000022         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_616797, whole genome shotgun sequence.
ACCESSION   DASUNT010000022 DASUNT010000000
VERSION     DASUNT010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616797"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFX94_00390"
     CDS             complement(<1..301)
                     /locus_tag="VFX94_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766663.1"
                     /translation="MALLVSGCAAMRSYDAELYVTLERASSGAVDEAIRLLESNNTKG
                     DKDLLYYLELGMLQRLAGRYPESQKAWKSAAARIEAQSAGGFIEATNFVRGVSSYV"
     gene            complement(340..1185)
                     /locus_tag="VFX94_00395"
     CDS             complement(340..1185)
                     /locus_tag="VFX94_00395"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX5766664.1"
                     /translation="MARSSLVLLALLAGCTTSPITGREQYIVVSESQAVSQSAAAYSQ
                     MMADLNKKKKIETGTPRVQKVKEITDKLVKEAVRVRPDAASWAWEVQVIDDPKTVNAF
                     CMAGGKMAIYTGMWDQLKASDDEIAQVMGHEIAHALADHTRERMSIAMTTAVATTAAA
                     VIVGTRDNSGPGAGLALTGAQLAAVLAIQLPNSRQGESEADQIGIELAARAGYDPKAA
                     VTLWQKMGKLGGGKSPEFLSTHPSPENRAARLAELGTQVQPLYEAARAGKPTDTPKLI
                     TFPRK"
     gene            1277..1444
                     /locus_tag="VFX94_00400"
     CDS             1277..1444
                     /locus_tag="VFX94_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766665.1"
                     /translation="MLPLRPGRKLPDEPAFKNPPQWIHEAGKWLFSRRETRKRELIRL
                     IDRMSPGKKEK"
     gene            complement(1475..>2595)
                     /locus_tag="VFX94_00405"
     CDS             complement(1475..>2595)
                     /locus_tag="VFX94_00405"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX5766666.1"
                     /translation="MQALRRAGSKNTVLMFDEIDKLGQGGFHGDPSSALLEVLDPEQN
                     SHFRDNYLGVDFDLSKIMFITTANMLDTIPGPLRDRMEIIQLPGYTEEEKLEIAKRYL
                     VKRQLEANGLDASKAEMSEEALRAIIEDYTREAGVRTLEREIGAVLRNAAMQIAEGRK
                     ERIVIGAQDLRSILGARRFESEIAQRTSVPGVATGLAWTPVGGDILFIEASRTSGTGK
                     LILTGQLGEVMKESAQAALTLAKVLTSQTLEKTDVHIHVPAGATPKDGPSAGVAMFLS
                     LISLLSAIPVRADVAMTGEISLRGLVLPIGGVKEKTLAALRAGIKTVMLPKRNEKDLE
                     DVPAEARAKLEFVFLERVEDAIRTAIGELPAAGERKAA"
BASE COUNT          386 a          867 c          845 g          497 t
ORIGIN      
        1 cgacatagct cgagacgccg cgcacaaagt tcgtggcctc gatgaatccg cccgcgctct
       61 gggcttcgat gcgcgccgcc gcgctcttcc aggccttctg gctttccggg tagcggccgg
      121 cgagcctctg cagcatgccg agctcgaggt agtagaggag atccttgtcg cccttggtgt
      181 tgttcgactc gagcagccgg atcgcctcgt cgaccgcgcc gctcgaggcg cgctccagcg
      241 tgacgtagag ctcggcgtcg tagctgcgca tcgcggcgca acccgagacg agcaaggcca
      301 cgccgcacaa cgaaagcgcg cggcgccagg cttgccggat cacttgcgtg ggaaggtgat
      361 gagcttgggc gtgtcggtag gcttgccggc cctggccgcc tcgtagagcg gctgcacctg
      421 cgtgccgagc tccgcgagcc gcgcagcgcg gttctccggc gaggggtgcg tggaaaggaa
      481 ttccggactc ttcccgccgc cgagcttgcc catcttctgc cacagcgtga cagccgcttt
      541 cgggtcgtag cccgcgcgcg cggcgagctc gatgccgatc tggtcggcct cggattcgcc
      601 ctggcggctg ttcggcagct ggatcgcaag caccgcggcg agctgcgccc cggtgagcgc
      661 gaggccggct cccgggcccg agttgtcgcg cgtcccgacg atgaccgcgg cagcggtggt
      721 ggcgaccgcg gtcgtcatcg cgatcgacat gcgctcgcgc gtgtggtcgg cgagcgcgtg
      781 cgcgatctcg tggcccatga cctgcgcgat ctcgtcatcg ctcgccttga gctggtccca
      841 catgccggtg tagatcgcca tcttgccgcc cgccatgcag aacgcgttga ccgtcttcgg
      901 atcgtcgatc acctgcactt cccacgccca gctcgcggca tcgggccgca cgcgcaccgc
      961 ctccttgacg agcttgtcgg tgatctcctt cactttctgc acgcgcggcg tgccggtctc
     1021 gattttcttc ttcttgttga ggtccgccat catctgcgaa tacgcggcgg ccgattgcga
     1081 taccgcctgg gattccgaaa cgacgatgta ctgctcgcgg cccgtgatcg ggctggtggt
     1141 gcagccggcg agcagcgcca gaaggactag agatgagcgc gccatagagc cgaaccctgt
     1201 tgaggtctct gattcgagag gagattttac ttcgtttact tcgcctttcg ggtggcatag
     1261 actcttcttg cccgccatgc tgccccttcg cccaggccgc aagctccccg acgaaccggc
     1321 gttcaagaat cctccgcagt ggatccacga ggcgggaaag tggcttttct cccggcgcga
     1381 aacgcgcaaa cgcgagctca tccggctgat agaccggatg agccccggca agaaagagaa
     1441 gtaatacctg tcttaagaag gggtcaggac ccttttacgc cgctttgcgc tcgccggccg
     1501 cgggcagctc gccgatcgcg gtgcggatcg catcctccac gcgctcgagg aacacgaact
     1561 cgagcttggc gcgcgcttcg gccggcacgt cctcgaggtc cttctcgttg cgcttgggca
     1621 gcatcacggt cttgattccc gcgcgcaagg ccgcgagcgt cttttccttc acgccgccga
     1681 tcgggagcac gaggccgcgg agcgagatct cgcccgtcat cgcgacatcg gctcgtaccg
     1741 gtattgcgct cagcagcgaa atcaggctga ggaacatggc gacgcccgcg ctcggcccgt
     1801 ccttcggcgt cgcgcccgcc ggcacgtgga tgtggacgtc ggtcttttcc agcgtttggg
     1861 acgtcaatac ctttgcaaga gtgagggcag cctgggccga ttccttcatc acctcgccga
     1921 gctggcccgt gaggatgagc ttgccggtgc ccgaggtgcg cgaagcctcg atgaagagaa
     1981 tgtcgccgcc caccggcgtc caggcgaggc cggtcgccac gcccggcacg gaagtgcgct
     2041 gggcgatctc gctctcgaag cgccgcgcgc cgagaatcga gcgcaggtcc tgcgcgccga
     2101 tgacgattct ctcctttctt ccttcggcga tctgcatcgc ggcgttgcgc agcacggcgc
     2161 cgatctcgcg ctcgagggtg cgcactccgg cctcgcgcgt gtagtcctcg atgatggcgc
     2221 gcagcgcctc ctcgctcatc tcggctttcg aagcatcgag cccgttcgcc tcgagctgcc
     2281 gcttcaccag gtaacgtttc gcgatctcga gcttttcttc ctccgtgtag ccggggagct
     2341 ggatgatctc catgcggtcg cgcagcggcc ccgggatcgt gtcgagcatg ttggcggtgg
     2401 tgatgaacat gatcttcgac agatcgaagt ccacgccgag gtagttgtcg cggaagtgtg
     2461 aattctgctc cgggtcgagc acttcgagca gcgccgagga cgggtcgccg tggaagccgc
     2521 cctggccgag cttgtcgatc tcgtcgaaca tcagcacggt gttcttcgag cccgcgcggc
     2581 gcagcgcctg catca
//
LOCUS       DASUNT010000023         2243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_528974, whole genome shotgun sequence.
ACCESSION   DASUNT010000023 DASUNT010000000
VERSION     DASUNT010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528974"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="VFX94_00410"
     CDS             <1..1016
                     /locus_tag="VFX94_00410"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766667.1"
                     /translation="DEIAACAFAIGASYAGKCTVTITSGPGYSLKQEAIGLAVMSEIP
                     LVVINVQRGGPSTGQPTKAEQGDLMSAIFGSHGDAPKVVIAASTIEDCFYSVITARRI
                     AETFNMVVVVLTDANLATSQQPFPRPEFDPAWMAPPADQSAVPEGLKPYDWDAATGLA
                     RRFIPGQPNGMHTVTGLAHDRESHVAYDPEINEESIHFRSLKLAALQRTLEAPTIFGP
                     AQGDLLAIGWGSTKGAIEEAVESLQREGRKVSSMHLRFLQPLAPGIKEAMSGFKKVMT
                     IEGNWSDRPEDQLIDETNRRYSALATLLRARYLVDVDCWSEVRGQPIKPATIREALRR
                     KLP"
     gene            1013..1927
                     /locus_tag="VFX94_00415"
     CDS             1013..1927
                     /locus_tag="VFX94_00415"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5766668.1"
                     /translation="MNDIACVLRLYDEKYELEDYQGGVPRWCSGCGDNAILASVQRLC
                     RDEGLRPEKTVFVSGIGCSSRFPHYMRTYGFHGIHGRALPVAEGVRLARPDLHVFVNT
                     GDGDCCSIGAAHWIHAVRYNMRMSVFLHDNQIYGLTKKQASPTSPRGTKSNTTPRGSA
                     LEALNPLTVTLGVQNVSFVAQAVDWMPDVLYEIVRAAFHHKGFSFVRIIQRCPEWLPD
                     QWEPYIKDPKRVLRLDKSGHDASNLHRAREMASQMDPIPVGILYRNPDVPCYEDLRRS
                     PKLRTAEMVRAGLEAELDKFTVWPEHAR"
     gene            complement(2061..>2243)
                     /locus_tag="VFX94_00420"
     CDS             complement(2061..>2243)
                     /locus_tag="VFX94_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766669.1"
                     /translation="CITVGDFSICSKPHWEAGKGVIATTANLSLAPERLCASLLVFAL
                     EGVIDQRGQGIDFSFF"
BASE COUNT          406 a          760 c          731 g          346 t
ORIGIN      
        1 aggacgagat cgcggcgtgc gcgttcgcga tcggcgcctc ctacgccggc aagtgcacgg
       61 tgacgatcac ctccggcccg ggctattcgc tcaagcagga ggcgatcggg ctcgcggtga
      121 tgagcgagat cccgctcgtg gtgatcaatg tgcagcgcgg cggcccgagc accggccagc
      181 cgaccaaggc cgagcagggc gacctgatga gcgcgatctt cggcagccac ggcgacgcgc
      241 ccaaggtggt gatcgcggcg agcacgatcg aggactgctt ctactcggtg atcacggcgc
      301 ggcgcatcgc cgagaccttc aacatggtgg tggtggtgtt gacggatgcc aacctcgcca
      361 cctcgcagca gcccttcccg cgcccggagt tcgacccggc gtggatggcg ccgccggccg
      421 accagagcgc ggtgcccgag gggctcaagc cctacgactg ggacgccgct acggggcttg
      481 cgcggcgctt catcccgggg cagccgaacg gaatgcacac ggtcaccggc ctcgcgcacg
      541 accgcgagag ccacgtcgcc tacgacccgg agatcaacga ggagtcgatc cacttccgta
      601 gcctcaagct cgccgcgctc cagcgcacgc tcgaggcgcc gacgatcttc ggcccggcgc
      661 agggtgatct gctcgcgatc ggctggggca gcaccaaggg cgcgatcgag gaagcggtgg
      721 aatcgctgca acgcgagggc cgcaaggtgt cctcgatgca tctgcgcttc ctgcagccgc
      781 tcgcgccggg aatcaaggaa gcgatgagcg gcttcaagaa ggtgatgacg atcgagggca
      841 actggtccga ccggccggaa gaccagttga tcgacgagac caaccgccgg tactccgcgc
      901 tcgcgactct gctgcgcgcg cgctacctcg tggacgtgga ctgctggagc gaagtgcgcg
      961 gccagccgat caagcccgcc acgatccgcg aagcgcttcg caggaaactc ccatgaacga
     1021 tatcgcgtgc gttctgcggc tctacgacga gaaatacgag ctcgaggact accagggcgg
     1081 cgtgccgcgc tggtgttcgg gctgcggaga caacgccatc ctcgcctcgg tgcagcgcct
     1141 gtgccgcgac gaaggcctgc ggccggagaa gaccgtgttc gtgtcgggca tcggctgctc
     1201 gagccggttc ccgcactaca tgcgcactta cggcttccac ggtatccacg gccgcgcgct
     1261 gccggttgcc gagggcgtgc gcctggcgcg gcccgacctg catgtgtttg tgaacacggg
     1321 cgacggcgac tgctgttcga tcggcgccgc gcactggatc catgccgtgc gctacaacat
     1381 gcgcatgagc gtgttcctgc acgacaacca gatctacggg ctcaccaaga agcaggcctc
     1441 gcccacctcg ccgcgcggca caaaaagcaa caccactccg cgcggcagcg cgctcgaagc
     1501 gctgaatccg cttaccgtga cgctcggcgt gcagaatgtg tcgttcgtcg cgcaagcggt
     1561 ggactggatg cccgacgtcc tctacgagat agtgcgcgcc gcgttccacc acaagggctt
     1621 ctcgttcgtg cggatcatcc agcgctgccc cgagtggctg cccgaccagt gggaaccgta
     1681 catcaaggat ccgaagcggg tgctgcgcct cgacaaaagc ggccacgatg cgtccaacct
     1741 ccaccgcgcg cgagagatgg cctcgcagat ggacccgatc ccggtcggca tcctttaccg
     1801 caacccggac gtgccctgct acgaggacct gcgccgcagc ccgaagctgc gcaccgcgga
     1861 aatggtgcgc gcgggcctcg aagccgagct cgacaagttc accgtgtggc ccgagcatgc
     1921 acgctgacct ccaggcccag ctcgccttcc atcttaccgg gcgcagtctc ccgccgatgg
     1981 agccctttga gctcatcccg gcgctcctcg cgcgttacag agatctgacg gcgctcagat
     2041 acgactttcc tctggtgctt tcaaaagaag gaaaagtcga ttccctgtcc acgctggtcg
     2101 atcacgccct cgagggcaaa gacgagaaga ctcgcgcaca ggcgctcagg cgcgagcgag
     2161 agattcgcag tggtagcaat cacgcccttg ccagcttccc agtgcggctt gctgcagatg
     2221 gagaagtcgc cgactgtgat gca
//
LOCUS       DASUNT010000024         3613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_617445, whole genome shotgun sequence.
ACCESSION   DASUNT010000024 DASUNT010000000
VERSION     DASUNT010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617445"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFX94_00425"
     CDS             <1..239
                     /locus_tag="VFX94_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3400 domain-containing protein"
                     /protein_id="HEX5766670.1"
                     /translation="RFSKEESLRKSMKELNSEKVKVLTSCPSCLQGLARFEDDIGAQA
                     DYIVVELARRMLGENWLPEFVAKANAGGIERVLL"
     gene            236..592
                     /locus_tag="VFX94_00430"
     CDS             236..592
                     /locus_tag="VFX94_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEX5766671.1"
                     /translation="MSERVATSIILHQKSRVMEIAFSDGRSFRLPYELLRVYSPSAEV
                     RGHGPGQEVLQTGKRAVDIRSLEPVGSYAVQPQFSDGHNTGIYSWEYLYELGENQDEL
                     WSQYLAKLKAAGASRG"
     gene            576..1277
                     /locus_tag="VFX94_00435"
     CDS             576..1277
                     /locus_tag="VFX94_00435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5766672.1"
                     /translation="MRAGDKAQTVRRVFDSVAERYDVMNDLMSLGLHRAWKATAVAVA
                     RPRPGERVLDIATGSGDLALSLSRKVSPGGEVWITDVNRRMLERGRDRLLDRGLALPA
                     VQCDAERLPFPAEHFDCVTVGFGLRNMTRKEAALAEMARVLKPGGRLVVLEFSKVWKP
                     LERAYDWYSFKVLPWLGDKVAGDADAYRYLAESIRVHPDQAALAEMLRGAGLSAVEVF
                     NLAAGVVAIHRGYRF"
     gene            1306..2124
                     /locus_tag="VFX94_00440"
     CDS             1306..2124
                     /locus_tag="VFX94_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HEX5766673.1"
                     /translation="MSKWLIASAVFVFSGIFLATEVEARRLGGGRTVGAQRNVTTPPA
                     QTPAKPAQQQAAPAQQGAQQAAPGATGSRWAPILGGLALGGILGYLFGGSGLFGFLLL
                     ALLAIAAVVGVRALMRRRAEVPQPVQFAGMRETMAVPVKNALPASSARARVPAGFDAS
                     GFLRAAKHNFLRLQAANDAGRLEEIREFTTDELYQELKSELDQKGETEVLGLEAELLE
                     LATEGERHWASVRFSGSVREARGAAPEPFAEVWNLVKPADGSSGWLLAGIQQMH"
     gene            2124..2735
                     /locus_tag="VFX94_00445"
     CDS             2124..2735
                     /locus_tag="VFX94_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766674.1"
                     /translation="MTSDPERGLTPIFSEATPAVAFCFLLNRLLAAEAWARRRLAPFA
                     GETLELRAPPLPALRLRILEGGTLEPGSGDPGLTMTLTPQLLPALARGSEHVARTVDV
                     RGNDKLAAEVLLLARHLRWDAEEALSRLIGDVAAHRVAGAGRALIAWHEDSARRLIAA
                     LGDYVTDETQVLAHRAELDALAESLARLRDGIARLEKRIERLD"
     gene            2728..>3613
                     /locus_tag="VFX94_00450"
     CDS             2728..>3613
                     /locus_tag="VFX94_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX5766675.1"
                     /translation="MTKAGRLARILWVGLRFGLHEFVPQAAGSLLVRALAGRTHEPRG
                     QRLREALETLGPIYVKFGQVLSTRRDLLPVDIADELAKLQDQVPAFPSELAVAEVERS
                     LGSTVDELFAEFDVSPVASASIAQVHLARLHDGADVAVKVLRPGVEAAVARDLTLLDT
                     AAGLAERLWADGKRLRPREVVAEFARHVEHELDLMREAANASQLGRNFADSPLLIVPA
                     VHWDLCAQRVMVMERMHGTPISQVRTLREKGIDIPALARAGVEIFFTQVFRDGFFHAD
                     MHPGNIRVADDGRYIALDF"
BASE COUNT          549 a         1208 c         1267 g          589 t
ORIGIN      
        1 tgcgcttcag caaggaggaa agcctgcgca agagcatgaa ggaactgaat tccgagaagg
       61 tcaaagtgct gacttcctgc ccttcgtgcc tgcagggtct cgcgcgcttc gaggatgaca
      121 tcggtgcgca ggccgactac attgtggtcg agctcgcgcg ccgcatgctc ggcgagaact
      181 ggctgccgga gttcgtcgcc aaggcgaacg cgggcggcat cgagcgtgtc cttttatgag
      241 cgagcgcgtc gcgacgagca tcatccttca ccagaagtcg cgcgtcatgg agatcgcgtt
      301 ctccgacgga cgcagcttcc ggctgccgta cgagctcttg cgcgtgtact cgccctcggc
      361 cgaggtgcgc ggccacggcc cgggccagga ggtcctgcag acgggcaagc gcgcggtcga
      421 tatccgttcg ctcgagcccg tgggctccta cgcggtgcag ccgcagttct ccgatgggca
      481 caataccggg atctactcct gggagtacct ctacgagctc ggcgagaacc aggacgagct
      541 ctggtcgcag tacctcgcaa agctcaaggc cgccggtgcg agcaggggat aaggcgcaga
      601 cggtaaggcg ggtgttcgac tcggtcgccg agcgctacga cgtgatgaac gacctcatgt
      661 cgctcggact gcatcgcgcc tggaaggcga ccgcagtcgc ggtggcgcgc cctcgaccgg
      721 gcgagcgcgt cctcgacatc gcgaccggca gcggcgatct cgcgctatcg ctctctcgca
      781 aggtcagccc gggcggcgaa gtgtggatca cggacgtcaa ccggcgcatg ctcgagcggg
      841 gacgcgaccg gctgctcgac cgcgggctcg cgcttcccgc ggtgcagtgc gacgccgagc
      901 ggctgccttt tccggcggag catttcgact gcgtcacggt gggcttcggg ctgcgcaaca
      961 tgacgcgcaa ggaagcggcg ctcgccgaga tggcgcgcgt gctcaagccc ggcgggcggc
     1021 ttgtcgtgct cgagttctcc aaggtgtgga agccgctcga gcgcgcctac gactggtatt
     1081 ccttcaaggt gctgccatgg ctgggcgaca aggtggcggg cgacgcggac gcgtaccgct
     1141 accttgccga atcgatccgc gtgcatcccg accaggcggc gctcgcggag atgctgcgcg
     1201 gcgctgggct ttccgctgtc gaagtgttta acctcgctgc tggtgtggtt gccatccatc
     1261 gcgggtatcg tttttgatag cattgcggat tcggaggaac gcaacatgag caagtggctg
     1321 atcgcatccg cagtcttcgt cttcagcggg atttttctcg caaccgaggt ggaagcgcgc
     1381 cgtctcggcg gcggccgcac cgtcggtgcg cagcgcaacg tgacgacccc gcccgcgcag
     1441 acgcccgcca agccggcgca gcagcaggcg gcgcctgcac agcaaggcgc gcagcaggcg
     1501 gcgccgggag cgaccggcag ccgttgggcg ccgatcctcg gcgggcttgc ccttggcgga
     1561 atcctcggct acctcttcgg cggcagcgga cttttcggct tccttctgct cgcgctgctc
     1621 gcgatcgccg ccgtggtcgg cgtgcgcgct ctgatgcggc gccgggcgga ggtaccgcag
     1681 ccggtccaat tcgccggaat gcgcgaaacc atggccgtcc cggtaaagaa tgcgctgccc
     1741 gcgtcgagcg cgcgagccag ggtcccggcc gggttcgacg cgtcgggctt cctgcgcgcg
     1801 gccaagcaca acttcctgcg gctgcaggcg gcaaatgacg ccggccggct ggaggaaatc
     1861 cgcgagttca ccaccgacga gctctaccag gagctgaaga gcgagctgga tcagaaaggc
     1921 gagaccgagg tcctcggtct cgaagccgag ctgctcgagc tcgccaccga aggcgagcgt
     1981 cactgggcaa gcgtgcgctt ctccggcagc gtgcgcgagg cgcgcggcgc cgcgccggag
     2041 cccttcgccg aagtgtggaa cctcgtgaag cccgcggacg gctcgagcgg gtggctcctg
     2101 gccggcatcc agcaaatgca ttgatgacgt cagaccccga gcggggtctg accccgattt
     2161 tttccgaggc cacccccgcg gtggcctttt gttttctgct gaaccggttg cttgccgccg
     2221 aggcctgggc tcggcggcgg cttgcgccgt tcgccggcga gacgctcgag ctgcgcgcac
     2281 caccgcttcc cgcgctacgc ttgcgcatcc tggagggggg aacgctggaa ccagggagcg
     2341 gggatccggg cctcactatg acgttgacgc cgcagctcct cccggcgctg gcgcgcggca
     2401 gcgagcatgt cgcaagaaca gtagacgtgc gcggcaacga caagctcgcc gccgaggtgc
     2461 tgctgctcgc gcggcacctg cgctgggacg ccgaggaggc gctttcccgc ctgatcggcg
     2521 acgtggccgc tcaccgcgtc gccggcgccg gccgcgcgtt gatcgcttgg cacgaggatt
     2581 ccgctcgtcg cctcatcgca gcgctcggcg actatgtgac cgacgagacg caggtactcg
     2641 cgcaccgcgc ggagctcgac gcgctcgccg aatcgctcgc gcggctgcgc gacgggatcg
     2701 caaggctcga aaagcggatc gagcggcttg actaaggccg gccggctcgc gcgcatcctg
     2761 tgggtcggcc tgcgcttcgg gctgcacgag ttcgtcccgc aggcggcggg aagcctcctg
     2821 gtgcgggctc tcgccggccg cacgcacgag ccacgcggcc agcggctgcg cgaggcgctg
     2881 gagacgctcg gtccgatcta cgtcaagttc gggcaggtgc tctccacccg gcgcgatctc
     2941 cttccggtgg acatcgccga cgagctggca aagctgcagg accaggtgcc cgcgtttcct
     3001 tccgagctcg cggtggccga agtggagcgc tcgctcggca gcaccgtcga cgaactcttt
     3061 gctgaattcg acgtgagtcc ggtggcaagt gcctcgatcg cgcaggtcca cctcgcgcgg
     3121 cttcatgacg gcgccgacgt cgctgtgaag gtgctgcggc ccggggtcga ggccgcggtc
     3181 gcgcgcgacc tcactctgct cgacacggcg gcgggcctcg ccgagcggct ttgggccgac
     3241 ggcaagcgcc tgcgaccgcg ggaggtcgtg gccgagttcg cgcgccatgt cgagcacgag
     3301 ctcgacctca tgcgcgaggc ggcgaacgca agccagctcg ggcgcaactt cgcggactcg
     3361 ccgctcctta tcgtgccggc ggtgcactgg gatctttgcg cgcaacgcgt gatggtgatg
     3421 gagcgcatgc acggcacgcc gatctcgcag gtccggacgc tgagagaaaa gggaatcgac
     3481 atcccggctc tcgcgcgcgc cggcgtagag atcttcttca cgcaagtctt tcgcgacggt
     3541 tttttccacg ccgacatgca tcccggcaac atccgggtcg ccgacgacgg ccgctacatc
     3601 gccctcgact tcg
//
LOCUS       DASUNT010000025         2522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_529616, whole genome shotgun sequence.
ACCESSION   DASUNT010000025 DASUNT010000000
VERSION     DASUNT010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529616"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1252
                     /locus_tag="VFX94_00455"
     CDS             278..1252
                     /locus_tag="VFX94_00455"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5766676.1"
                     /translation="MPAPDIRRYLKHGVLPQLRAFEAAARLGTLTRAAQELHMAQATA
                     SVQVKKLSETLGVPLFEQVGNRLQLTDAGQAVYASCGQLFGALSAMESTLSPMRGCSS
                     GGVRVAAPSTARHFASRLIAAFVSQNPGVEASLQIHDRIALIERLSRNADDLYLFVEP
                     PGDREVVSQALVPNPLVVLASADHPLAAGTDIPFARLAQEPLVMREPGSGIRMIVLRL
                     FARHGIAPKIRLELASDEAIREAVLAGLGLAVLPRYTLGIDPMLTKLVCLDVEGFPLE
                     SYWHFVYPVGKRPSPAARVFMDFARAEAKALFRDCLRAPRSAAEISSA"
     gene            complement(1164..1982)
                     /locus_tag="VFX94_00460"
     CDS             complement(1164..1982)
                     /locus_tag="VFX94_00460"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX5766677.1"
                     /translation="MHPMLNIAVRAARRAGSIINRAALDGGGLEVRAKQKNDFVTRVD
                     QAAEQSIIDVVRKAYPDHALLAEESGHSAGSAEYQWIIDPLDGTTNFIHGFPQYCVSI
                     AIRHRGALAHGVVYDPVRNELFTASKGRGAFLNDRRIRVSKCTRLGDALVGTGFPFRE
                     AARLELYVGQLETLMKASSGVRRAGAAALDLAYVACGRLDAFWEMGLSAWDMAAGALM
                     ILEAGGLVGDLRGEAGYLESGEICAATPKIFPPLIEALSGSRGTAPSPRRAQSP"
     gene            2007..>2522
                     /locus_tag="VFX94_00465"
     CDS             2007..>2522
                     /locus_tag="VFX94_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX5766678.1"
                     /translation="MQAIRFVLCRPSHPGNIGAAARAMKTMGLTDLRLVAPERFPAPE
                     AQWMATNAVDVLQNAVIHHNLLDAIRDCAGAFAMSARGREWSPQVLDVRTAAMRASAI
                     AGPVAFVFGNETAGLTNDEMFACQHLVHIPANPQFSSLNLAQAVQVAAYELRMAVDLA
                     IPFSRAEKAATV"
BASE COUNT          397 a          866 c          837 g          422 t
ORIGIN      
        1 gtcgtgcatg acaaaacctc cgctcgggcg agggactcgc gcactatcgc cgcgacgcag
       61 cgcacaaaag aattagaact tcatgaagca cgccatcaca cttcttgata gcccggggca
      121 gagctgctat taatatgcgt actgcagagg ggagtagctt tcggcggcca accccggccg
      181 ctgtggcgcg atcgtcaaac cgggcctgga ccccggtcgc gccagcaccc accgtgcgag
      241 cgagaccttt gcctcgacga gaggtaaagg attcgccatg cccgcaccgg atatccggcg
      301 ctatctgaaa cacggagtgc tgccgcagct gcgagcattc gaagcggctg cgcgcctggg
      361 cacgctcacg cgcgcggcgc aggagctgca catggcccag gccaccgctt cggttcaggt
      421 gaagaagctc tccgagacgc tcggcgtgcc gctgttcgag caggtgggca accggctgca
      481 gctcaccgat gccgggcagg cggtctacgc gagctgcggc cagcttttcg gcgcgctatc
      541 ggcgatggag agcacgctga gcccgatgcg cggctgctcc tcgggcggcg tacgggtcgc
      601 cgcgccgtcc accgcgcgcc atttcgcctc gcggctcatc gccgccttcg tgtcgcagaa
      661 cccgggcgtc gaagcctcgc tgcagattca tgatcgcatt gcgctcatcg agcggctgag
      721 ccgcaacgcc gacgacctgt acctcttcgt cgagccgccc ggagatcgcg aggtcgtgtc
      781 ccaggcgctg gtgccgaacc cgctcgtcgt cctggcaagc gccgaccatc cgctcgccgc
      841 cgggacggat atcccgttcg cccgcctcgc gcaggagccg ctcgtgatgc gcgagcccgg
      901 ctccggcatc cggatgatcg tgctgcggtt attcgcgcgg cacggcatcg ctccgaagat
      961 ccggttggag ctcgcctccg acgaggcgat ccgcgaggcg gtgctggcgg ggctcggcct
     1021 cgcggtgctg ccgcgctata cgctcggcat cgatcccatg ctcaccaagc tggtttgcct
     1081 cgatgtggag ggattcccgc tggagagcta ctggcacttc gtctacccgg tcggcaagcg
     1141 gccctcgccg gccgcgcggg ttttcatgga ctttgcgcgc gccgaggcga aggcgctgtt
     1201 ccgcgactgc ctgagagcgc ctcgatcagc ggcggaaata tcttcggcgt agcggcgcag
     1261 atttcgcccg actccaggta gcccgcttcg ccgcgcaggt cgcccaccag tccgccggct
     1321 tccaggatca tgagcgcgcc ggcggccatg tcccaggccg agagccccat ctcccagaac
     1381 gcgtcgaggc ggccgcaggc gacataggcg aggtcgagcg ccgccgcgcc ggcgcgcctc
     1441 accccagaac tcgctttcat cagcgtttcg agttgcccca catagagctc gagccgcgca
     1501 gcctcgcgga aagggaaccc ggtcccgacc agggcgtcgc ccagccgcgt gcacttcgac
     1561 acgcggatgc gccggtcgtt gagaaaggcg ccgcggccct tggaagcggt gaacagctcg
     1621 ttgcgcaccg ggtcgtacac cacgccgtgc gcgagcgcgc cgcggtggcg gatcgcgatc
     1681 gacacgcagt actgcgggaa gccgtggatg aaattggtgg tgccgtcgag cgggtcgatg
     1741 atccactggt actccgcgct tcccgcgctg tggcccgatt cctccgcgag cagcgcgtgg
     1801 tcggggtacg ccttgcgcac cacgtcgatg atcgactgtt ccgcagcctg gtccacgcgc
     1861 gtcacgaaat cgtttttctg cttggcgcgc acttcgaggc cgccgccgtc gagcgcggcg
     1921 cggttgatga tcgaccctgc gcggcgcgcg gcgcggaccg cgatgttgag catcgggtgc
     1981 atcgggcgcg tattctagac gtcgtcatgc aggcgatccg ctttgtcctg tgccgcccga
     2041 gccaccccgg aaacatcggc gctgcggcgc gcgcgatgaa aaccatgggc ctcaccgact
     2101 tgcgccttgt agcgccggag cgctttccgg cgcccgaggc gcagtggatg gcgaccaatg
     2161 ccgttgacgt tctgcagaac gccgtcattc accacaatct gcttgacgcc ataagagact
     2221 gcgcgggcgc gtttgcgatg tccgcccgcg gccgcgaatg gtcgcctcag gtgctcgatg
     2281 tgcgaactgc ggcgatgcgc gcaagcgcga tcgccggccc ggtcgcgttc gtgttcggca
     2341 atgaaaccgc aggactgacc aacgacgaga tgttcgcctg ccagcacctg gttcacatcc
     2401 ccgcgaatcc gcaattcagc tcgctcaatc tcgcgcaggc ggtgcaggtg gcagcctacg
     2461 agctgcgcat ggcagtcgac ttggccattc ccttctcgcg ggcggagaag gcggcgacgg
     2521 tg
//
LOCUS       DASUNT010000026         2147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1055846, whole genome shotgun sequence.
ACCESSION   DASUNT010000026 DASUNT010000000
VERSION     DASUNT010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055846"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VFX94_00470"
     CDS             complement(<1..220)
                     /locus_tag="VFX94_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766679.1"
                     /translation="MDRYEGGRVQVFSTCPQSKDVPRANYLGQVADVARWSEEAGCTG
                     MLVYTDNSIVDPWLTSQVILQSTATLAPL"
     gene            309..1142
                     /locus_tag="VFX94_00475"
     CDS             309..1142
                     /locus_tag="VFX94_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEX5766680.1"
                     /translation="MRVALVCEESAGARVLENLARDAHEIVALVTSPHSPAWNLGVKL
                     GVRPLAARRVREPDFADVLASCGAQLLLNIHSLYIVPQPALRAPPLGAYNLHPGPLPG
                     CAGLNAPSWAIYRGEPRHGVTVHRMEAGIDTGPIAFQDWFPLEESDTGLSVALRCARR
                     GVELVRKLVQILARDPAGLELTPQDLAQRRYFGREVPQGGRIDWNAPARRIYDFVRAC
                     DYQPFASPWGTPVAQLGGREVEVQKVALTGERSHAAPGSVRDDNLVATADEWLLLKKW
                     K"
     gene            complement(1126..2019)
                     /locus_tag="VFX94_00480"
     CDS             complement(1126..2019)
                     /locus_tag="VFX94_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX5766681.1"
                     /translation="MIVSIGEPMVEFNQTKPGERTFLQGFGGDSSNMIIAAARSGARA
                     AYVTRIGDDEFGRMMLELWKREGVDASGVIVDREAPTGAYFVTHGAQGHVFSYLRSGS
                     AASRLRPGDLPNLDKASVVHASGISMAISQSACDTVLAAFSKAQTISFDSNLRLKLWP
                     LARARAMIGAAAAMADYFFPSIEDARQLSGRQSIDENLAWAHRLGAQTVFLKLGPEGV
                     VVSDGRRSERIPGMTVKAIDATGAGDCFCGATLARLAAGDSIWEAARYANAAAALATT
                     GYGAVDPLPRPEQVRKLISTS"
BASE COUNT          345 a          733 c          705 g          364 t
ORIGIN      
        1 ccagcggggc gagcgtggcg gtgctctgca ggatcacctg ggaggtgagc catggatcga
       61 cgatcgagtt gtcggtgtac accagcattc cggtgcaacc cgcctcctcg ctccagcgcg
      121 ccacgtcggc gacctgcccg aggtagttag cgcgcggcac gtccttcgac tggggacagg
      181 tggagaacac ctgtacccgt ccaccttcgt agcggtccat gcgtcaagaa ttgaaccgca
      241 gccagatttc tcccggacca tcctgtcaac cgcctttcag actatgaatg gcgggagaat
      301 ttccgggaat gcgggtcgcg ctcgtctgtg aggagtcggc gggcgccagg gtgctcgaaa
      361 acctggcgcg ggacgcccat gagatcgttg cactcgttac gtcgccgcac tcgcccgcct
      421 ggaacctcgg cgtgaagctc ggggtgcgcc cgcttgcggc gcggcgagtg cgcgagcccg
      481 actttgccga cgtcctggcg agctgcggcg cgcagctttt gctcaatatc cactcgctct
      541 acatcgtgcc gcagcccgcg ctgcgcgcgc cgccgctcgg tgcgtacaac cttcacccgg
      601 ggccgctacc cggctgtgcc ggcctgaacg cgccgagctg ggcgatctac cgcggcgagc
      661 cgcgtcacgg cgtcacggtg catcggatgg aggcgggaat cgataccggt ccgatcgctt
      721 ttcaggattg gtttcctctc gaagagagcg ataccgggct gagcgtcgcc ttgcgttgcg
      781 cccgcagagg cgttgagctc gtgcgcaagc tcgtgcagat cctcgcgcgc gatcccgcag
      841 ggcttgaatt gacgcctcag gacctcgcac agcggcgcta cttcggacgc gaggtgccgc
      901 agggcgggcg catcgactgg aatgcgccgg cgcggcgcat ctacgatttc gtgcgcgcct
      961 gcgactacca gcccttcgcc tcgccgtggg gcacgccggt agcgcagctc ggcggacgcg
     1021 aggtagaggt gcagaaagtc gcgctcaccg gcgagcgctc ccatgcagcg ccgggaagcg
     1081 tgagagacga caacctcgtc gccaccgccg acgagtggct gctgctcaag aagtggaaat
     1141 gagcttgcga acctgctccg ggcggggcag cggatcgact gcaccgtatc ccgtggtcgc
     1201 cagcgccgct gccgcattcg catagcgcgc ggcttcccag atcgagtcgc ccgccgcaag
     1261 gcgcgcgagc gtcgcgccgc agaagcagtc gcccgcgccc gtggcatcga tcgcctttac
     1321 cgtcatcccc ggaatgcgct cgctgcgcct tccgtcgctg accaccacgc cctcgggccc
     1381 gagcttgaga aaaacggtct gcgctccaag gcggtgcgcc cacgcgaggt tctcatcgat
     1441 agactgcctc ccgctcagct gccgggcatc ttcgatgctg ggaaagaaat agtccgccat
     1501 ggccgcggcc gcgccgatca tcgcccgcgc gcgcgccagc ggccagagct tgagtcgcag
     1561 gttcgagtcg aaggaaatgg tttgtgcctt cgaaaaagcc gcgagcacgg tatcgcacgc
     1621 cgattgcgag atcgccatgc tgatgccgga tgcatggacc accgatgcct tgtccagatt
     1681 aggcaagtcc ccggggcgca gccggctcgc agccgagccg gagcgcaggt agctgaacac
     1741 atggccttgc gctccatggg tgacgaagta cgcgccggtc ggcgcctcgc ggtcgacgat
     1801 gacgccgctc gcgtccacgc cttcgcgctt ccagagctcg agcatcatgc ggccgaactc
     1861 gtcgtcgccg attcgggtga cgtacgcggc acgcgcgccg ctgcgcgcgg cggcgatgat
     1921 catgttcgaa gagtcgccgc cgaagccctg caggaaggtc ctttccccgg gcttcgtctg
     1981 gttgaactcc accatcggct cgccgatcga gacgatcatt cgaggaaccg ccgcgcgtgg
     2041 gcgacaacgc gggcgcggtc gcccgccgcc agcgctttct gatcgacgat gttgttcccc
     2101 actcccacga tgagcgctcc cgcctcgaag tacttcctca tgttgtc
//
LOCUS       DASUNT010000027         5107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_529730, whole genome shotgun sequence.
ACCESSION   DASUNT010000027 DASUNT010000000
VERSION     DASUNT010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529730"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VFX94_00485"
     CDS             <1..561
                     /locus_tag="VFX94_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX5766682.1"
                     /translation="AGHLKIIDRAKDVGKLADGTLFAPKYLENKLKFFPYIKEAVCFG
                     DRRDCASAFINIDVTAVGDWAERRGLAYSGYPDLAAKPEVYELIRGCVEKVNRDLAGD
                     AKLANSQIRRFLVLPKELDADDGELTRTRKVRRSFIAEKYAPLVQALYDGSGQCGFNA
                     EVKFEDGRRSSLRAELKIWDASLVPA"
     gene            937..1734
                     /locus_tag="VFX94_00490"
     CDS             937..1734
                     /locus_tag="VFX94_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5766683.1"
                     /translation="MRKRREILGTATTDAYLRVQDIHLNFGGVKALTGVSFQVHRGEI
                     TSIIGPNGAGKTSMINVVSGFYRPTRGRIFFDGADRTHLPAHRVAQLGFARTFQNIAL
                     FKGMSTLDNLMAGRRFRHNLLLDALYVGPAKREELRQRAEIERIIDFLEIQSIRKKPV
                     GRLPYGLQKRVELGRALAMQPKVLLLDEPMAGMNVEEKEDMARFILDVNDQWGTTIVL
                     IEHDMGVVMDISNHVVVLDHGAKIAEGTPAEVRADPVVIRAYLGVAH"
     gene            1736..2635
                     /locus_tag="VFX94_00495"
     CDS             1736..2635
                     /locus_tag="VFX94_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5766684.1"
                     /translation="MDASFFFEVLIGGLLAGVMYSLVAIGFVLIYKASGVFNFAQGAM
                     VFFAALTYVSLVERGWPFWLALAAVLALMIVLGFAIERVVLRPLVNQPQITLFMVTIG
                     LAYFFEGVAQGVWGAQVHALEIGIQDAPFTVAGISVSQFDLFASGTAALLVLVLGVFF
                     QKTKTGRALRAVADDHQAALSVGIQLKYVWAVVWAAAGFVALVAGLLWGARTGVQFAL
                     TFTALKALPVLILGGFESIPGAIVGGLIIGASEKLAEVYIGSSIGLNIGTGIENWFPY
                     MLALVFLLVRPEGLFGEKRIERI"
     gene            2638..3702
                     /locus_tag="VFX94_00500"
     CDS             2638..3702
                     /locus_tag="VFX94_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5766685.1"
                     /translation="MFYRLAGQFKSSYAADQQIFPIRQDRFFVVGLMLVAFVVIPLVA
                     NDYWLGAILIPFLILGLAALGLNILTGYTGQLSLGTAGFMAVGAFAAFNFILRVPQMP
                     FLLALFLAGVCAALIGILFGLPSVRIKGFYLAVATLAAQFFIEWVLTKFGWFSNYNPS
                     GVITAQAIEILGYEFNTPRSKYLLTLGVVAALALAAKNLARTQTGRYWMAVRDMDLAA
                     EVTGIDIARTKLVAFAVSSFYCGVAGALWAYAYLGAVEPQAFTLDRSFQILFMIIIGG
                     VGTVLGAFLGSAFIVLLPIGLNVGAHTLFPGVSTDLMSNLELVIFGALIVFFLAVEPH
                     GLARLWQIGKEKLRLWPFPH"
     gene            3719..5032
                     /locus_tag="VFX94_00505"
     CDS             3719..5032
                     /locus_tag="VFX94_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5766686.1"
                     /translation="MKAKIYLAVAAALCAGTALAQPNEQFIPMLSYRVGPYAAGGTGF
                     FGGYIDYLDMLNRRDGGIGGVKLTWQECETEYKNDRGVECYERLKKSGPTGATVFAPL
                     STGITYSLIERATADKIPVISMGYGRADASDGRVFPYVFPLVTNYWSQNTAKIKFIGM
                     KEGGVDKLKGKKIVNLYHGSAYGKETIPILDAQAQKYGFTVTHIEVPHPGNEQQSQWL
                     QVRSIKPDWVILRGWGVMNPTALKAAQKVGYKTERIVGVWWSGAEEDVLPAGDAAKGY
                     IAAAFHPSGTEFPVVQEILKHVYAGGKRGNMEDKGRIGQQYFNRGVIQGILTAEAIRT
                     AQTKFGQRPLSGEEMRWGIENLKVDEKRLKELGAYNLMQPMKVSCFDHEGGGAVKFQQ
                     WDGKQWKVISDWIASDQSIVRPQIEKSAAQYASEKKLAPRDCPKG"
BASE COUNT          858 a         1698 c         1666 g          885 t
ORIGIN      
        1 gccgggcacc tcaagatcat tgaccgcgcg aaagacgtcg gcaagctcgc cgacggcact
       61 ctgttcgcgc ccaagtacct ggagaacaag ctcaagttct tcccgtacat caaggaagcc
      121 gtgtgcttcg gcgaccggcg cgactgcgcc agcgccttca tcaacatcga cgtcaccgcg
      181 gtcggagact gggctgagcg gcgcggcctc gcctattcgg gctacccgga cctcgccgcc
      241 aagcccgagg tctacgagct catccgcggc tgcgtcgaga aggtgaaccg cgacctcgcc
      301 ggcgatgcga aactcgcgaa ctcgcagatc cgccgcttcc tcgtgctgcc gaaggagctc
      361 gacgccgacg acggcgagct cacgcgcacg cgcaaggtgc gccgctcgtt catcgccgag
      421 aaatacgcgc cgctcgtgca ggcgctgtac gacggctccg ggcagtgcgg cttcaacgcc
      481 gaggtgaagt tcgaggacgg gcgccggagc agcctgcgcg ccgagctcaa gatctgggat
      541 gcgtctttgg tgcctgcgta gatggaccag agtacgttcg cggcccgtct taccttccat
      601 gattccatcg agaccatgga agtggatttt tccaccttcg cgttcaccga cgcgacggtg
      661 gtcgatgcct tctacgacca catcgacgcg cgcgtcgcgg ccaccggacg gcgctggtat
      721 ttcttggtcc tatacgaggg gtgcgagatc gcgccggagg cctgggagcg cttcgccgcg
      781 cgcggcaagc acgccaacat tacctacggc ctgggcacgg tgcgcgtgca tccgagcgag
      841 cgcacgcgcg agacgatccg cagccgcgcc gagcgcgaaa ccttccgcgc caacattttc
      901 gatacgcgcg accaggcgat ggtcgcgctc ggcgaaatgc gcaagcggcg cgagattctc
      961 ggcaccgcga ccaccgacgc ttacctgcgg gtgcaggaca tccacctgaa tttcggcgga
     1021 gtaaaggcgc tcaccggcgt gagcttccag gtgcaccgcg gcgagatcac ctcgatcatc
     1081 ggccccaacg gggccggcaa gacctcgatg atcaacgtgg tgagcggctt ctaccgcccg
     1141 acgcgcgggc gcattttttt cgacggcgcc gaccgcacgc acctgccggc gcaccgcgtc
     1201 gcgcagctcg gcttcgcgcg cacgttccag aacatcgccc tcttcaaggg catgagcacg
     1261 ctcgacaacc tgatggccgg gcggcggttc cgccacaacc tgttgctcga tgcgctgtac
     1321 gtcggcccgg cgaagcgcga ggagctgcgc cagcgcgccg agatcgagcg gatcatcgac
     1381 ttcctcgaga tccagtcgat ccgcaaaaag ccggtggggc ggctccccta cggcctgcag
     1441 aagcgcgtcg agctcgggcg cgcgctcgcc atgcagccca aggtcctgct gctcgacgag
     1501 ccgatggccg gcatgaacgt cgaagagaag gaggacatgg cgcgcttcat cctcgacgtg
     1561 aacgaccagt ggggcaccac gatcgtgctg atcgagcacg acatgggcgt ggtgatggac
     1621 atctcgaacc acgtcgtggt gctcgaccac ggcgccaaga tcgccgaagg cacgccggcc
     1681 gaagtgcgcg ccgacccggt ggtgatccgg gcctatctcg gggtggctca ctgacatgga
     1741 tgcgagtttc ttcttcgagg tgctgatcgg cgggctgctc gccggggtca tgtactcgct
     1801 cgtcgcgatc ggcttcgtgc tgatctacaa ggcctcgggc gtattcaact tcgcgcaggg
     1861 cgcgatggtg ttcttcgccg cgctcacgta tgtgagcctg gtcgagcgcg gctggccgtt
     1921 ctggctcgcg ctcgccgcgg tgctcgcgct catgatcgtg ctcggcttcg ccatcgagcg
     1981 cgtggtgctg cggccgctcg tcaaccagcc gcagatcacc cttttcatgg tcacgatcgg
     2041 gcttgcctac ttcttcgagg gcgtggcgca gggcgtctgg ggcgcgcagg tgcacgcgct
     2101 ggagatcggc atccaggacg cgcctttcac cgtggccggc atcagcgtga gccagttcga
     2161 tctcttcgcc tcgggcaccg cagccctcct cgtgctggtg ctcggggtct tcttccagaa
     2221 gaccaagacc gggcgcgcgc tgcgcgccgt ggccgacgac caccaggccg cgctctcggt
     2281 cgggatccag ctcaagtacg tgtgggcggt ggtgtgggcg gcggccggct tcgtcgcgct
     2341 ggtggcgggc ctgctgtggg gcgcgcgcac cggcgtgcag ttcgccctca ccttcaccgc
     2401 gctcaaggcg ctcccggtgc tgattctcgg cggcttcgag tcgatccccg gcgcgatcgt
     2461 cggcggcctc atcatcggcg cgagcgagaa gctcgccgag gtctacatcg gctcctcgat
     2521 cggcctcaac atcggcaccg gcatcgagaa ctggttcccg tacatgctgg cgctcgtctt
     2581 cctgctcgta cggccggagg gcctgttcgg cgaaaaacgc atcgagcgga tctgaccatg
     2641 ttctatcgcc tcgccgggca gttcaagtcg agctacgccg ccgaccagca gatcttcccg
     2701 atccggcagg accggttttt cgtagttggc ttgatgcttg tcgcctttgt cgtcattccc
     2761 ctggtggcga atgactactg gctgggcgcg atcctcatcc cgttcctgat cctcggcctc
     2821 gccgcgctcg ggctcaacat cctcaccggc tacaccgggc agctctcgct cggcaccgcg
     2881 ggcttcatgg cggtcggggc attcgcggcg ttcaacttca tcctgcgcgt gccccagatg
     2941 ccgttcttgc tggcgctttt cctcgcgggg gtctgtgcgg cactcattgg catcctgttc
     3001 ggcctgccga gcgtgcgcat caagggcttc tacctcgcgg tggcgacgct cgcggcgcag
     3061 tttttcatcg agtgggtgct gaccaagttc ggctggttct cgaactacaa cccctcgggc
     3121 gtgatcaccg cgcaggcgat cgagatcctc ggctacgagt tcaacactcc gcgcagcaaa
     3181 tatctgctca cgctcggcgt cgtcgccgcg ctcgcgctcg ccgcgaagaa cctggcgcgc
     3241 acccagaccg ggcgctactg gatggcggtg cgcgacatgg acctcgccgc cgaggtgacc
     3301 ggcatcgaca tcgcgcgcac gaagctcgtg gcgttcgcgg tgagctcgtt ctactgcggt
     3361 gtcgccggcg cgctctgggc ctacgcctat ctcggcgcgg tcgagccgca ggccttcacg
     3421 ctcgaccgct cgttccagat tctcttcatg atcatcatcg gcggggtcgg caccgtgctc
     3481 ggcgccttcc tcggctcggc gttcatcgtg ctcttgccga tcgggctcaa cgtcggcgcg
     3541 cacacgctct tccccggcgt gagcaccgac ctgatgtcca atctcgagct cgtcatcttc
     3601 ggcgcgctca tcgttttctt cctcgcggtc gagccgcacg gcctggcaag gctctggcag
     3661 atcggcaagg agaagctgcg gctatggccg ttcccgcact gaatcgaagg aggatgagat
     3721 gaaagcgaaa atctacctcg cggtcgccgc ggcgctgtgc gccgggaccg cgctcgcgca
     3781 acccaacgag cagttcatcc ccatgctctc gtaccgcgtc gggccgtacg ccgccggcgg
     3841 caccggcttc ttcggcggct acatcgacta tctcgacatg ctgaaccgcc gcgacggcgg
     3901 catcggcggc gtgaagctga cctggcagga atgcgagacc gagtacaaga acgaccgcgg
     3961 cgtcgagtgc tacgagcgcc tgaagaagag cggcccgacc ggcgccacgg tgttcgcgcc
     4021 gctctcgacc ggcatcacct attcgctgat cgagcgcgcc accgccgaca agatcccggt
     4081 gatctccatg ggctacggcc gcgccgacgc ctccgacgga cgcgtgttcc cgtatgtctt
     4141 tccgctggtg accaactact ggagccagaa caccgccaag atcaagttca tcggcatgaa
     4201 ggaaggcggg gtggacaagc tcaagggcaa gaagatcgtg aacctgtacc acggctcggc
     4261 ctacggcaag gagaccatcc cgatcctcga cgcacaggcg cagaagtacg gcttcacggt
     4321 gacccacatc gaggtgccgc acccgggcaa cgagcagcag tcgcagtggc tgcaggttcg
     4381 ctcgatcaag ccggactggg tgatcctgcg cggctggggt gtcatgaatc ccactgcgct
     4441 caaagcggcc cagaaggtgg gctacaagac cgagcgcatc gtcggcgtgt ggtggagcgg
     4501 ggcggaggaa gatgtgctgc cggcgggcga cgcggccaag ggctacatcg ccgcggcgtt
     4561 ccatccctcg ggcaccgagt tcccggtcgt gcaggagatc ctcaagcacg tctatgccgg
     4621 cggcaagcgc ggcaacatgg aggacaaggg ccgcatcggc cagcagtact tcaatcgggg
     4681 agtgatccag ggaatcctga ccgccgaggc gatccgcacc gcgcagacca agttcggcca
     4741 gcgcccgctc agcggagagg agatgcgctg gggcatcgag aacctgaagg tcgacgagaa
     4801 gcggctgaag gagctcggcg cctacaacct gatgcagccg atgaaggtct cgtgcttcga
     4861 ccacgagggc ggcggcgcgg tgaagttcca gcaatgggac ggaaagcagt ggaaggtgat
     4921 cagcgactgg atcgcgagcg accagtcgat cgtgcgaccg cagatcgaaa aatcggcggc
     4981 acagtacgcg tccgagaaga agctcgcgcc gcgcgactgc ccgaaaggct aggcgcatga
     5041 acccgctgct gcgcgtcaac aacatcgagg tcatctacga ccacgtgatc ctcgtgctga
     5101 agggggt
//
LOCUS       DASUNT010000028         2357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_3711, whole genome shotgun sequence.
ACCESSION   DASUNT010000028 DASUNT010000000
VERSION     DASUNT010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3711"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VFX94_00510"
     CDS             complement(<1..746)
                     /locus_tag="VFX94_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5766687.1"
                     /translation="MDFDLSQVAQTLAIYALPVLFAITLHEAAHGYVARHFGDMTAHA
                     QGRISLNPVRHIDIIGTIVVPLVILLLSYGKFLFGWARPVPVNYSALRKPRQHMAWVA
                     AAGPGANLAMALAWALVLRASVSMTSRADAWMQVAQQYGVSGLIDVVMRHGNGAAEFL
                     VGVSAAGVLVNLLLMLLNLLPILPLDGGRIVASLLPTRAAWRFAKLEPFGVPLLLLLL
                     FVPVYGSSALSWILSPLLFESEALLRAMVLL"
     gene            complement(765..1391)
                     /locus_tag="VFX94_00515"
     CDS             complement(765..1391)
                     /locus_tag="VFX94_00515"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX5766688.1"
                     /translation="MAQYFSIHPTHPQKRLVRQAADIVRRGGLIAYPTDSCYALGCHR
                     GNAAGAARLRRIRGIDERHRLTLMCRDLSEISRYAIVDDTQFALLKSATPGSYTFILR
                     ARRELPRRLTRKKTIGVRVPGHAVAHALLAELDEPMVSATLLLPGAAAPLSDPPEIRQ
                     LLEHELDLVIDAGPCGTAPSTVIDLTEKTPIVLRAGKGSLAPFVVEGV"
     gene            complement(1391..2257)
                     /locus_tag="VFX94_00520"
     CDS             complement(1391..2257)
                     /locus_tag="VFX94_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5766689.1"
                     /translation="MKQSESRFVEIRGLRYHVRHWPAPGAPKVVLLHGWMDVSASFQF
                     VVDELQQDWDVYAPDWRGYGLTEWGKADCYWFPDYIADLEFLLADIDAANPLNIVGHS
                     LGANVAALYAGVRPARVARFANLEGFGMAPTRAEQAPKRYARWLEELRDPPGFRAYES
                     YSELAQRLRSSNPRLSMEKAQFLARHWGREEGKTVVLRSDPAHKIVNPVLYRYEEVRA
                     CWSEVATPVLWVEGAESATLERLKLGAQDVAERRTAFRDLRYERLADAGHMLHHDQPA
                     ALARLLERFLAR"
BASE COUNT          402 a          778 c          827 g          350 t
ORIGIN      
        1 agcagcacca tggcgcgcag gagcgcttcc gactcgaaaa gcagcgggct caggatccag
       61 ctgagcgcgc tcgagccgta gaccggcacg aaaagcagca gcaggagcag cggcacgccg
      121 aaaggctcga gcttcgcgaa gcgccacgcc gcccgcgtgg gcagcaggct cgccacgatg
      181 cgcccgccgt cgagcggcag gatcggaagc agattgagca gcatgaggag caggttgacc
      241 agaacgcccg cggcgctcac cccgacgagg aactcggcgg cgccgttgcc atgcctcatc
      301 accacgtcga tcaggccgct cacgccgtat tgctgcgcga cttgcatcca ggcgtcggcg
      361 cggctcgtca tgctcaccga tgcgcgcagc acgagcgccc acgcgagcgc catggcgaga
      421 ttcgcgccag ggccggcagc cgcgacccac gccatgtgtt gacgcggctt gcgcagcgcg
      481 gagtagttta ccggcacggg cctcgcccag ccgaaaagga acttgccgta ggacaggagc
      541 aggatgacga gcggaaccac gatggtgccg atgatgtcga tgtgccgcac cgggttgagg
      601 ctgatgcgcc cctgcgcgtg cgcggtcatg tcgccgaagt gacgggcgac gtagccgtgg
      661 gcggcttcgt gaagcgtaat ggcgaaaagt accgggagcg cgtaaatggc cagggtctgg
      721 gcgacctggc ttagatcgaa gtccattctg cggccggaag tctatcagac gccctccacc
      781 acgaagggcg cgagcgagcc cttgccggcg cgcagcacga tcggggtttt ttcggtgaga
      841 tcgatgaccg tgctcggcgc cgtgccgcac gggcctgcgt cgatgacgag atcgagctcg
      901 tgctcgagca gttgcctgat ttcaggagga tcggagagcg gcgccgccgc gcccgggagg
      961 agcaaggtcg cggacaccat cggctcgtcg agctcggcga gcaaggcatg ggcgacggca
     1021 tggcccggca cgcgcacgcc gatagtcttc ttgcgcgtca ggcggcgcgg cagctcgcgt
     1081 cgcgcgcgca ggatgaacgt gtagctgccc ggggtcgcgc tcttcagcag ggcgaactgc
     1141 gtgtcgtcca cgatcgcgta gcgcgagatc tccgacaagt cgcggcacat gagcgtaagc
     1201 cggtgccgct cgtctatgcc tcggatgcgg cgcaggcgcg ctgcgccggc agcgttgccg
     1261 cggtggcagc ccagcgcata gcaggagtcg gtcgggtatg cgatcaggcc gccgcgccgc
     1321 acgatgtccg ccgcctgccg cacgagacgc ttctgcggat gcgtggggtg gatggagaaa
     1381 tactgcgcca tcagcgtgcg aggaagcgct ccagcaagcg cgcgagcgcg gcgggctggt
     1441 cgtggtgcag catgtgaccc gcatcggcca gccgttcata tcgcaggtcg cggaacgcgg
     1501 tgcggcgttc ggcgacgtcc tgcgcgccaa gcttcagccg ctcgagcgtt gcggattctg
     1561 cgccctcgac ccacagcact ggcgttgcga cctcgctcca gcaggcgcgc acctcttcgt
     1621 atcggtagag caccgggttg acgatcttgt gcgcgggatc gctgcgcagc acgactgtct
     1681 tgccctcctc gcggccccag tgccgcgcaa gaaactgcgc cttctccatg gaaagcctcg
     1741 gattactgct gcgcaagcgc tgcgccagct ccgaatagct ctcgtaggct ctgaagccag
     1801 gcgggtcgcg cagctcctcg agccagcgcg cgtagcgctt cggcgcctgc tcggcccgcg
     1861 tcggcgccat gccgaagccc tcgaggtttg caaacctcgc cacgcgcgcg ggccgcacgc
     1921 cggcgtacaa cgcggccacg tttgcgccca ggctgtggcc gacgatgttg agaggattgg
     1981 ccgcgtcgat gtccgccagg agaaactcca ggtcggcgat gtagtcggga aaccagtagc
     2041 agtcggcttt tccccattcg gtgagcccgt agccgcgcca gtcgggagcg tacacgtccc
     2101 agtcttgctg cagctcgtcc acgacaaact ggaacgacgc cgatacgtcc atccagccgt
     2161 gcagcaagac gaccttcggc gctccgggcg cgggccagtg gcgcacgtgg tagcgcagcc
     2221 cgcgaatctc gacgaagcgt gactcgcttt gcttcacgcg aacggaaact ccacctgggg
     2281 tgagcggccg gacacgaggt cggcgagcgc cgcgccggag ccgcaggcga gcgtccagcc
     2341 gagcgtgccg tggccgg
//
LOCUS       DASUNT010000029         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_267014, whole genome shotgun sequence.
ACCESSION   DASUNT010000029 DASUNT010000000
VERSION     DASUNT010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267014"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..526
                     /gene="yjgA"
                     /locus_tag="VFX94_00525"
     CDS             29..526
                     /gene="yjgA"
                     /locus_tag="VFX94_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEX5766690.1"
                     /translation="MDQQIVSKTRRKREMHELQALGAQLARLPEAQLKSIELPEELRE
                     ALLDAKRMTSREAKRRQMQYVGRLMRGLDPEPIRARLGEIEGSSARATARQRRLESWR
                     ERLLDDDDALTLFAAEHPGAELQGLRTLIRNARKEALEGKPPRAYRELFRVLKAIESS
                     TAVRG"
     gene            498..947
                     /locus_tag="VFX94_00530"
     CDS             498..947
                     /locus_tag="VFX94_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HEX5766691.1"
                     /translation="MNLQPLFGAEITLAAAQELGETPQGRRRIIGITGGRFSGERLSG
                     RVLPGGADWQVIRSDGVADLDARYTLETADGALIYVRNRGYRHGPAEILRRLASGENV
                     DPGLYYMRTTPRFETGDPRYAWLNRIICVASGARRRDAVELQVFEVK"
     gene            944..1498
                     /gene="mog"
                     /locus_tag="VFX94_00535"
     CDS             944..1498
                     /gene="mog"
                     /locus_tag="VFX94_00535"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HEX5766692.1"
                     /translation="MNQDLLIGLVSISDRASAGVYKDEGIPSLKDWLASAIASPAWRD
                     ETRLIADEQPVIEATLRELVDDKGCSLVLTTGGTGPAPRDVTPEATLAVADKVMPGFG
                     EQMRAVSLKYVPTAILSRQVAVIRKKALIINLPGQPKAIRETLDGVFAAVPYCVDLIG
                     GPYIETNEAVVKAFRPKSAIRKKP"
     gene            complement(1495..1887)
                     /locus_tag="VFX94_00540"
     CDS             complement(1495..1887)
                     /locus_tag="VFX94_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766693.1"
                     /translation="MKRILVADLPQMEQRYSSALAGWDIAFARTMGQAREALAGGRYD
                     LVAVGVYFDDSQIFDLVRAVRRDPRHGDVPIVCVRSQTGCTAVSSRTLESAVKDLGAE
                     QLIELAALGDDAAGEGALRAAAEQLVKD"
     gene            1913..>2428
                     /locus_tag="VFX94_00545"
     CDS             1913..>2428
                     /locus_tag="VFX94_00545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5766694.1"
                     /translation="MLTRPVLIFVMALAGCASALQSTPEVQYEPTPMSVVHVMLDLAL
                     VHSDDLVADLGCGDGRLVIEAVRRHGARGLCVDIDPRRIAEARSNAAAAGVAERITFR
                     NQDLFATDLRGVTVVMLFLSPRFNLKLRPKLERELASGARVVSHWHDMGDWPPKRTVR
                     VRSDGRERAVYL"
BASE COUNT          401 a          847 c          820 g          360 t
ORIGIN      
        1 ttcggcatgg gggcgctatg ataatcgcgt ggatcagcaa attgtcagca agacccgcag
       61 gaagcgcgaa atgcacgagc tgcaggcgct cggcgcgcag ctcgcgcgcc ttcccgaagc
      121 gcagttgaag tcgatcgagc tgcccgagga gctgcgcgag gcgctgctcg acgcgaagcg
      181 catgacgagc cgggaggcga agcgccgcca gatgcagtac gtcggccgct tgatgcgcgg
      241 cctggacccc gagccgatcc gcgcacgcct gggcgagatc gaaggcagct ccgcgcgcgc
      301 cacggcgcgc cagcgcaggc tcgagagctg gcgcgagcgc ctgctcgacg acgacgacgc
      361 gctcaccttg ttcgccgccg agcatccagg cgcggagctg caggggctgc gcacgctgat
      421 ccgaaacgcg cgcaaggagg cgctcgaggg caagccgccg cgcgcctacc gcgaactctt
      481 cagggtgctg aaagcgattg aatcttcaac cgctgttcgg ggctgagatc accctcgccg
      541 cggcgcagga gctgggcgag acgccgcagg gccggcggcg catcatcggc atcaccggcg
      601 ggcgcttcag cggcgagcgg ctctcgggac gcgtgctgcc gggcggcgcc gactggcagg
      661 tgatccgctc cgacggcgtc gcggacctcg acgcccgcta cacgctcgag accgccgacg
      721 gcgcgcttat ctatgtccgc aaccgcgggt accgccacgg ccctgcggaa atcttgagga
      781 gattggcttc cggcgagaac gtcgacccag gcctctacta catgcgcacc acgccgcgtt
      841 tcgagaccgg cgatccacgc tacgcgtggc tgaaccgcat catctgcgtc gcaagcggcg
      901 ctcgccgcag ggacgcggtc gaactccagg tcttcgaggt gaaatgaacc aggatcttct
      961 gatcgggctc gtctcgatca gcgacagggc ctcggcgggc gtctacaagg acgaagggat
     1021 tccgtcgctc aaggactggc tcgcctccgc gatcgcctcg cccgcgtggc gtgacgagac
     1081 tcgcctcatc gccgacgagc agccggtgat cgaggcgacg ctgagggagc tggtggacga
     1141 caagggctgt agcctggtgc tcaccacggg tggcaccgga cccgcgccgc gggacgtcac
     1201 gccggaggcc acgctggcgg tggcggacaa ggtgatgccc ggcttcggcg agcagatgcg
     1261 cgcggtgagt ctcaagtacg tgcctaccgc catcctttca cgtcaggtgg ccgtgatccg
     1321 aaaaaaggcg ctcatcatca acctgccggg acagcccaag gcgatccgcg agacgctcga
     1381 cggcgtgttc gccgcggtgc cctattgcgt agacctcatc ggcggcccgt acatcgagac
     1441 gaacgaggcc gtggtcaagg ccttccggcc gaaatcggcc attcgaaaga aaccctaatc
     1501 cttgacgagc tgttcggccg cggcgcgcag cgccccttcc ccggccgcat catcgccgag
     1561 cgccgcgagc tcgatgagct gctccgcacc gaggtctttt accgcgctct ccagggtgcg
     1621 gctgctgacc gcggtgcagc cggtctggct gcgcacgcag acgatcggca cgtcgccgtg
     1681 gcgcgggtcc cggcgcaccg cgcgcaccag gtcgaaaatc tgcgagtcgt cgaaatacac
     1741 gccgaccgcg acaaggtcgt agcgcccgcc ggcaagcgcc tcgcgcgcct gccccatggt
     1801 gcgcgcaaac gcgatgtccc agccggcgag ggcggacgaa tagcgctgct ccatttgcgg
     1861 caggtccgcg accaggatcc ttttcacgtt ctggtggaat actaacccgc ccatgctcac
     1921 tcgccctgtc ttgatcttcg tcatggcgct cgccgggtgc gccagcgcgc tccagtcgac
     1981 ccctgaagtg caatacgagc cgacgccgat gagcgtcgtg cacgtgatgc tcgaccttgc
     2041 cctggtgcat tccgacgacc tcgttgccga cctcggctgc ggcgacgggc gcctcgtgat
     2101 cgaggccgtg cgccgccacg gcgcgcgcgg cctgtgcgtc gacatcgatc ccaggcgcat
     2161 cgccgaggcg cggagcaacg cagccgcggc cggcgtcgcc gagcgcatca cctttcgcaa
     2221 ccaggacctc ttcgcgaccg acctgcgcgg cgtgaccgtc gtcatgctct ttctgtcgcc
     2281 gcgcttcaac ctcaagctgc gcccaaagct cgagcgcgag ctcgcgtccg gggcgcgcgt
     2341 agtctcgcac tggcacgaca tgggcgactg gccgccgaag cgcacggtgc gcgttaggag
     2401 cgacggccgc gagcgcgcgg tctacctc
//
LOCUS       DASUNT010000030         6381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_91810, whole genome shotgun sequence.
ACCESSION   DASUNT010000030 DASUNT010000000
VERSION     DASUNT010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91810"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1214)
                     /gene="sat"
                     /locus_tag="VFX94_00550"
     CDS             complement(48..1214)
                     /gene="sat"
                     /locus_tag="VFX94_00550"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX5766695.1"
                     /translation="MPSLVSPHGAGVLAPRALSGEALAAERERARSLPQLRISSRERG
                     DLIMLGIGGFTPLEGFMTRADWEGVCERYRTASGLFWPIPITLSAKEEIKTGKDLALV
                     DPENGEPLATMRVTECYAIDKARECQTVFRTTDAAHPGVAMVMAQPEFNLAGPVKVLS
                     DGGFKAKYGALFMTPAQTRAEFERLGWSRVAAFQTRNPMQRAHEYLVKVAIEVCDGVL
                     VHSLLGALKPGDIPAEVRTRAIAVLIEKYFRPGTVVQAGYPLDMRYAGPREALLHALF
                     RQNYGCSHLIVGRDHAGVGKYYGPFDAHKIFDEIPPGSLETRPLKMDVTFWCYRCHGM
                     ASGRTCPHGEKDQLQVSGTQLRKWLEEGSEVPAEFSRPEVAAILIAAERAKVPA"
     gene            complement(1273..1578)
                     /locus_tag="VFX94_00555"
     CDS             complement(1273..1578)
                     /locus_tag="VFX94_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5766696.1"
                     /translation="MRTFLAVLAIAVSLPAAAQSTKVHWYGQAAFRIETPAGGVILID
                     PWLKAPTNPDKESVEKLSRVDYILVTHGHWDHIGDAVEIGKKTGAVLVMKPGESRNF"
     gene            complement(1608..3101)
                     /gene="glpK"
                     /locus_tag="VFX94_00560"
     CDS             complement(1608..3101)
                     /gene="glpK"
                     /locus_tag="VFX94_00560"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX5766697.1"
                     /translation="MKYVLALDQGTTSSRAIVFDAEGRAVTAAQREFRQIFPQPGWVE
                     HDPKEIFSSQRDTARQALRESGISSKELMAVGIANQRETTIVWDRQTGEPIHNAIVWQ
                     DRRTAAQCAELKAAGAENLVRSRTGLVIDPYFSGTKIAWLLDHVPGARGRAERGELAF
                     GTVDTWLAWHLTGNRTHVTDVSNASRTLLFDIHTNDWDAELLELLRVPRAILPDVHPS
                     AHAFGMLPAGVLGEPQVIGGMAGDQQAAMFGQACHHAGMAKNTYGTGCFMLLHTGDKV
                     VQSSSGLISTACAQTKGKEFALEGSVFIGGAVVQWLRDGLEFFKSSSEIERLAASVLD
                     SGDVYLVPAFTGLGAPYWDPDARGSISGLTRGTTRAHIARAALESIAFQSAELLEAMQ
                     KDSRQPLSELRVDGGAAANDLLMQFQADLLGVPVVRPRVLETTALGAAYLAGLTVDLW
                     KSRDELASHWKAERRFEPRMAPAERQARMSRWREAVARARSWAKVDS"
     gene            complement(3114..3725)
                     /locus_tag="VFX94_00565"
     CDS             complement(3114..3725)
                     /locus_tag="VFX94_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5766698.1"
                     /translation="MPAAALTVYIIDDDAAVRDSLSLMLGLAGYATALFADAEAFLAA
                     YRDDWAGCVVADLRLPGKSGLELQAELRARSSALPFILMTAHGDVPSARTAFQARAVD
                     FLEKPFDHAQLRSAIENAFSREQQRLARANELAKLATLTVREREVLEHAAQGLHAKEI
                     AAALGISPRTVEVHKTRIMEKLGARNIAELVRFALGTRDDGER"
     gene            complement(3707..5257)
                     /locus_tag="VFX94_00570"
     CDS             complement(3707..5257)
                     /locus_tag="VFX94_00570"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEX5766699.1"
                     /translation="MSVIRYLRDAAVFAPCYIALDWASYLDPVGPFNITPWNPQPALA
                     MAWMLLGGLGHAPAVFVTIVAADVLVRGAPGGPFITVLSSGVLAASYAALAWMLRELA
                     RGGVGLRTTRELTLFVAVTVGGTALAGAAFVGVLSGAALIPRAEVAGAWLRFWIGDAV
                     GILVTAPLLFAVADAARRAGLAALARRPETWAQASLLVGTLWLIFIGLPGEPALHFYL
                     LFVPIIWIAARGGMNGAVVATAIVQLGVVLGIHRTGAGSLPVLELQALVATLTLTGLF
                     LGMMVDERQRAAFNLRRSLRLAAAGEMAGALAHEVNQPLTALSNYGQSALVLLRRGDE
                     TARLQTIVEKMLAEAERAGEVVRRLRDFFRTGTTRLEPVPVEELVAAARRIGQRLIGE
                     RRVAFEVRGDRSLAPLYVDRLQVELILRNLIANSVDAVQATPDGRIHVSMTAEEPQRA
                     RIVVADNGPGIASASRESVFEPFVSGKPSGMGLGLAVSRAIAEAHGGSLEARAGEFHL
                     VLPCLPPR"
     gene            5321..5563
                     /locus_tag="VFX94_00575"
     CDS             5321..5563
                     /locus_tag="VFX94_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766700.1"
                     /translation="MMLNEFLDLKAWAEWFAALDREFMFLLALPFVVAIIGLWAWWVE
                     RDEEEEYEAPPAQPAQPERPRGERRGATRIPHHGLR"
     gene            5653..6144
                     /locus_tag="VFX94_00580"
     CDS             5653..6144
                     /locus_tag="VFX94_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEX5766701.1"
                     /translation="MNRPRLLETAALAASLFVFWLLLSGIYTPFLLLSGLGASIAVAL
                     LATRMEITDREGHPIHLAFAAVRYWPWLLKEIVKSGWQVARIIIDPRLPISPTLVRFR
                     PSQTSAVALATHANSITLTPGTITVDATAEEFLVHALTREGAAGVVGSEMDRRVSRLD
                     QRA"
     gene            6144..>6381
                     /locus_tag="VFX94_00585"
     CDS             6144..>6381
                     /locus_tag="VFX94_00585"
                     /inference="COORDINATES: protein motif:HMM:NF015993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HEX5766702.1"
                     /translation="MFSAAAVALLATLALLLVRALKGPTVFDRLLASNTIGNVAVLLL
                     AVIGFLTGRPDFLDLGLVYGLLNIVGTIAVLKFFR"
BASE COUNT         1003 a         2193 c         2187 g          998 t
ORIGIN      
        1 ctttggtccc gtggcaccgt catcctgagc gaagcgaacg atccgcccta cgccggaacc
       61 ttcgcccgct cagccgcgat caggatcgcc gccacctcgg gccgcgagaa ctcggccggc
      121 acctcgctgc cctcctccag ccacttgcgc agctgcgtgc ccgacacctg cagctggtcc
      181 ttctcgccgt gcgggcaggt gcgccccgag gccatgccgt ggcagcggta gcaccagaat
      241 gtgacgtcca tcttgagcgg ccgcgtctcg agcgagcccg gcgggatctc atcgaagatc
      301 ttgtgcgcgt cgaacggccc gtagtacttg cccacgcccg cgtggtcgcg acccacgatg
      361 agatgcgagc agccgtagtt ctggcgaaag agcgcgtgca gcagcgcctc gcgcggcccg
      421 gcgtagcgca tgtcgagcgg atagcccgcc tgcaccacgg tgccgggacg gaaatacttt
      481 tcgatcagca ccgcgatcgc gcgcgtgcgc acctcggcgg ggatgtcgcc gggcttcaat
      541 gcgccgagga gcgagtgcac gagcacgcca tcgcaaacct cgatcgccac cttgacgagg
      601 tactcgtgcg cgcgctgcat cgggttgcgc gtctggaatg cggcgacgcg cgaccagccg
      661 agccgctcga actcggcgcg cgtctgggcc ggcgtcatga aaagcgcgcc gtacttcgcc
      721 ttgaagccgc cgtcggaaag caccttgacc gggccggcga ggttgaactc gggctgcgcc
      781 atcaccatcg cgacgccggg gtgtgctgcg tcggtcgtgc gaaacaccgt ttggcactcg
      841 cgcgccttgt cgatcgcgta gcactcggtc acgcgcatgg tggcgagcgg ctcgccgttt
      901 tccgggtcga ccaacgccaa gtccttcccg gtctttattt cttcttttgc tgaaagagta
      961 atcgggattg gccagaagag gcccgaagcc gtgcggtagc gctcgcacac gccttcccag
     1021 tcggcgcgcg tcatgaagcc ttccagcggc gtgaagccgc cgatgccgag catgatgaga
     1081 tcgccgcgct cgcgcgagct gatgcgaagc tgcggcaggc tgcgcgcgcg ctctctctcg
     1141 gcggcgaggg cttcgccgct caaggcacgc ggcgcgagga cgccggcgcc gtgcggagaa
     1201 actaggcttg gcacgcagtc attgtcggga aggcgagacg ggccccgcca atcagaacct
     1261 ttgattcccg gatcagaagt tccgcgactc gcccggcttc atcaccaaga cggcgccggt
     1321 cttcttgccg atctcgacgg cgtcgccgat gtggtcccag tgcccgtggg tgacaaggat
     1381 gtaatcgacg cgcgagagct tttccaccga ctccttgtcc gggttggtgg gcgccttcag
     1441 ccacgggtcg atgaggatga ccccgccggc aggcgtttcg atccgaaacg ccgcctgtcc
     1501 ataccagtgg accttcgtgg actgcgcagc cgccggtagc gagaccgcga ttgcaagcac
     1561 cgccagaaac gttctcatct tgtttttcct ccctccagaa agctgcttca ggaatctact
     1621 ttagcccagg agcgcgcgcg ggcgacggcc tcgcgccagc gcgacatccg cgcttgcctt
     1681 tccgcaggcg ccatgcgcgg ctcgaagcgg cgctcggcct tccagtgcga ggcgagctcg
     1741 tcgcgcgatt tccaaagatc cacggtcagg cccgcaaggt aggcggcgcc gagcgccgtg
     1801 gtttcgagca cgcgcggccg caccaccggc acgccgagca ggtcggcctg gaactgcatc
     1861 agcaggtcgt tggcggctgc gccgccatct acccgaagct ccgacagcgg ttgccgggaa
     1921 tccttttgca tcgcctcgag cagctcggcg ctctgaaagg cgatcgactc gagcgcggcg
     1981 cgcgcgatgt gggcgcgggt cgtgccccgc gtgaggcccg agatcgaacc gcgcgcgtcg
     2041 ggatcccagt agggcgctcc caggccggtg aaggccggaa cgaggtacac gtcgccggag
     2101 tcaagcacac tggccgccag gcgctctatt tcggaggagc ttttgaaaaa ctccaggcca
     2161 tccctcagcc attgcacgac cgcgccgccg atgaacacgc tgccctcgag cgcgaactcc
     2221 ttgcccttgg tctgcgcgca ggcggtcgag ataaggccgc tcgaggactg cacgaccttg
     2281 tcgccggtgt gcagcagcat gaagcagccg gtgccgtacg tgttcttcgc catgccggcg
     2341 tggtggcacg cctggccgaa catcgccgcc tgctggtcgc ctgccatgcc gccgatgact
     2401 tgcggctcgc ccagcacgcc ggcgggcagc atgccgaagg cgtgcgccga tggatgcacg
     2461 tcgggcagga tcgcgcgcgg cacgcgcagg agctccagca gctccgcgtc ccagtcgttg
     2521 gtatgaatgt cgaaaagcag cgtgcgcgac gcgttcgaga cgtcggtgac gtgggtgcgg
     2581 ttgccggtga ggtgccaggc gagccaggtg tcgaccgtgc cgaaagcgag ctcgccgcgc
     2641 tcggcgcgcc cgcgcgcgcc cgggacgtgg tcgagcagcc acgcgatctt cgtgccgctg
     2701 aagtacggat cgatcacgag gccggtgcgg ctgcgcacca ggttctcggc gcccgccgct
     2761 ttgagctcgg cgcactgcgc ggccgtgcgc cggtcctgcc agacgatcgc gttgtggatc
     2821 ggctcgcccg tctggcggtc ccagacaatc gtggtctcgc gctggttcgc gatgccgacc
     2881 gccatcagct ccttggagga aatcccggat tcgcggagcg cttgcctggc cgtgtcgcgt
     2941 tgcgaggaga aaatttcctt cgggtcgtgc tcgacccagc cgggctgggg aaagatctgg
     3001 cggaactcgc gctgcgcggc cgtgaccgca cgcccctcgg cgtcgaacac gatcgcgcgc
     3061 gagctggtgg tgccctggtc gagggcgagg acgtacttca ccgaaaaatt ctatcagcgc
     3121 tcgccgtcgt cgcgcgtgcc gagcgcgaag cgcacgagct ccgcgatgtt gcgcgcgccg
     3181 agcttctcca tgatgcgcgt cttgtggacc tcgacggtgc gcgggctgat gcccagtgcg
     3241 gcggcgatct ccttcgcgtg caggccctgg gccgcgtgct cgagcacttc gcgctcgcga
     3301 acggtcagag tggcgagctt tgcgagctca ttcgcgcgtg cgaggcgctg ctgctccctg
     3361 gaaaaggcgt tctcgatcgc gctgcgcagc tgcgcgtggt cgaaaggctt ctcgaggaag
     3421 tccaccgccc gcgcctggaa cgcggtacgc gcggagggta cgtcgccgtg cgcggtcatg
     3481 aggatgaagg gcagggcgct ggagcgcgcg cggagctcgg cctgcagctc caggccgctt
     3541 ttccccggca ggcgcaggtc ggcgactacg cagcccgccc agtcgtcgcg gtaggcggcg
     3601 agaaaggcct cggcgtcggc gaagagcgcg gtggcgtagc ctgcgaggcc gagcatgagc
     3661 gaaagcgagt cacgcaccgc ggcgtcgtcg tcgataatgt agacggtcag cgcggcggca
     3721 ggcatggcag gacaagatgg aactccccgg cgcgcgcctc gagcgagccg ccgtgcgcct
     3781 cggcgatcgc gcggctcacc gcgagcccga ggcccatgcc gctcggcttg cccgagacga
     3841 aaggctcgaa cacgctttcg cggctggccg acgcgatccc cgggccgttg tcggcgacca
     3901 cgatgcgcgc gcgctggggc tcctcggccg tcatcgagac atggatgcgg ccatccggag
     3961 tcgcctggac ggcgtccacg gagttggcga tcaggttgcg cagaatgagc tccacctgca
     4021 ggcggtcgac gtagagcggc gcgaggctgc ggtcgccgcg cacttcaaac gcgacgcgcc
     4081 gctcgccgat caggcgctgg ccgatgcgcc gcgccgcggc gaccagctct tccaccggca
     4141 ccggctccag gcgcgtggtg ccggtgcgga agaagtcgcg caggcggcgc accacttcgc
     4201 cggcgcgctc ggcctcggcc agcatcttct cgacgatggt ttgcaggcgc gccgtctcgt
     4261 cgccgcgccg gagcagaacc agcgcggatt gtccgtagtt ggagagcgcc gtgagcggct
     4321 ggttgacctc gtgcgcgagc gcgcccgcca tctccccggc cgcggcgagg cgcagcgagc
     4381 ggcgcaggtt gaacgccgct cgctgccgct cgtccaccat catgccgaga aagaggccgg
     4441 tgagcgtaag cgtcgcgacc aacgcttgca gctcgagcac cggcagcgag cccgccccgg
     4501 tgcgatggat gccgagcacg acgcctagct ggacgatcgc ggtcgcaacc accgcaccgt
     4561 tcataccgcc gcgcgccgcg atccagatga tgggcacgaa aagcagatag aaatgcagcg
     4621 ccggttcccc gggcaggccg atgaaaatga gccagagagt gccgacgagc aggctcgcct
     4681 gcgcccaggt ctcgggtctg cgggcgagcg cggcgagccc cgcgcggcgc gccgcatcag
     4741 cgacggcgaa gagcagcggc gcggtgacga ggatgccgac cgcgtcgccg atccagaatc
     4801 gcagccacgc gccggccacc tcggcgcgcg gaatcagcgc cgccccgctc agtacgccga
     4861 cgaacgcggc cccggcgagc gcagtcccgc cgacggtcac cgcgacgaac agcgtcagct
     4921 cgcgcgtggt gcgcagcccg acgccgccac gcgcgagctc gcgcagcatc caggcgagcg
     4981 cggcatagct cgccgcgagc acgccggagc tgagcaccgt gatgaacggc ccgccgggcg
     5041 cgccgcgcac cagcacgtcc gccgcgacaa tggtgacgaa gaccgcgggc gcgtgcccca
     5101 ggccgccgag gagcatccac gccatggcga gcgccggttg cgggttccag ggcgtaatgt
     5161 tgaacggccc gaccggatcg aggtagctcg cccagtcgag cgcgatgtag cagggcgcga
     5221 acaccgcggc gtcgcgcaag tatcggataa cggacatagg tgaaaacact tacgcgctct
     5281 tgcgcatagg cgggcgctcc gggcgcggct attgtgcacc atgatgctca acgaatttct
     5341 ggacctcaag gcctgggcgg aatggttcgc cgccctggat cgcgagttca tgttcctgct
     5401 cgcgctgccg ttcgtcgtgg ccatcatcgg gctgtgggcc tggtgggtcg agcgcgacga
     5461 agaagaggag tacgaagcgc ccccggcaca accggcacag cccgagcgcc cgcggggcga
     5521 gcgccgcggc gcaacgcgga ttccgcatca cggcctgaga tagcaccgga gttgcagtag
     5581 tgcgccggcc cgccctcggg tcctcgaccc gacggcggga ttcgggcatt ctgtcgacgg
     5641 ccaagaatga caatgaatcg cccgcgctta ctcgagactg cagccctcgc ggcctcgctg
     5701 tttgtcttct ggctgctgct ctccggcatc tacacgccgt tcctcctgct ttcggggctg
     5761 ggcgcctcga tcgcggtcgc gttgcttgcg acgcgcatgg aaatcaccga ccgcgaaggt
     5821 caccccattc atctcgcttt cgccgcggtt cgctactggc cgtggctgct aaaggaaatc
     5881 gtcaaatccg gctggcaggt cgcgcgcatc atcatcgatc cgcgcctgcc gatcagcccg
     5941 acgctggtgc gttttcggcc gtcacagacg agcgcggtgg cgctcgcgac gcacgccaac
     6001 tccattaccc ttacgcccgg cacgatcacg gtggacgcca cggccgaaga gttcctggta
     6061 catgcgctca cgcgcgaagg ggcagccggc gtggtgggaa gcgagatgga ccgccgcgtg
     6121 agccggctcg accagcgcgc gtgatgttct ccgccgccgc tgtagcgctg ctcgcgacgc
     6181 tcgcgctgct actcgtgcgc gcgctcaagg gaccgaccgt cttcgatcgc ctgctcgcga
     6241 gcaacaccat cggtaacgtc gccgtgctcc tcctcgcggt catcggcttc ctgaccgggc
     6301 ggccggactt cctcgacctc gggctcgtgt acggactgct caacatcgtg ggcacgattg
     6361 cggtactcaa gttcttccgc c
//
LOCUS       DASUNT010000031         3678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_617970, whole genome shotgun sequence.
ACCESSION   DASUNT010000031 DASUNT010000000
VERSION     DASUNT010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617970"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VFX94_00590"
     CDS             <1..418
                     /locus_tag="VFX94_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766703.1"
                     /translation="SGTLRLWEVLALRGETARVLPDPVRSLCVLDAALEAGLRLIHGK
                     RMDAELHPARVRRLGAAGAEIESKAPVAVFGALQLVLAMEPALALDGKVIELTERDGI
                     ATALVRFTGLDWDTRARIETYARERRARGYDPGSKP"
     gene            415..1098
                     /locus_tag="VFX94_00595"
     CDS             415..1098
                     /locus_tag="VFX94_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX5766704.1"
                     /translation="MSPIIPALVALPAFAATQAQALDPAPRGRTNELSLNVQVVGSDD
                     YEFDGGASVRNDGGAGVALSIARNLNDHFAIGAEVAWSTSNFRARVAPGAGNSGGRFD
                     SEGDTESVALRLHATWYLLSRPITPFVTAGVGVIFFDAELDDSPPANTCWIYPWYGEV
                     CGASAPENTLTRLSYTAGAGLRWDLPPTWGHREGFLRLLLGGEWIDFEEASSPVGYWQ
                     VRLDFGARF"
     gene            complement(1095..1376)
                     /locus_tag="VFX94_00600"
     CDS             complement(1095..1376)
                     /locus_tag="VFX94_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766705.1"
                     /translation="MNPARHALILALALAACAAPSTYWEKPGATAEMVQQDNDQCLAN
                     ARSNSPQPALYGQTGTQTTRVLTLEEQRERSEVESFQKCMREKGYSAKR"
     gene            1375..3120
                     /locus_tag="VFX94_00605"
     CDS             1375..3120
                     /locus_tag="VFX94_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX5766706.1"
                     /translation="MPSIRAWLCLTLLVCGAALAQEPAPTKYRLRVEAPTKELRELLG
                     KGLQLARWQDDPQMTPELLRRLADEAVVEARQALAADGYFAALVSYSIDRDVSPWSVV
                     LHVEPGTRATVSGVEITFTGPASTDPEAAKHLARVRNEWLLRPGMAFTQEAWGEAKRE
                     AVRRLSAWRYAEARIASSRARVDAQANTAMLEITLESGRPFRFGGVEVRGNGRYDNSI
                     AANLSPVEPGTTYDREVLELYTRRLLEPGYFAGARAELAPEAATDGQAPVNVSVIEGH
                     SQNIEGGVSFTTDRGVRLEAAHRNADLLDSAWRGRASARWDELTQEARYDLDAPPLPG
                     ARWWSGFTAVKNELIQNEENFEFSGGIAHNWAGKGSPISIFASGHLENQQVSGQEVDN
                     RQAVFFGFRVGFRETDAVVLPRRGYFGQFTGGVAPENLSTQHFVRGTWRVTFLVPLGR
                     NDDLSMRAEAGLVVANSRAGIPSTFLFRTGGDQTVRGYAFESLGVREGDAVLGGRYMA
                     IGSVEYTHWFSPVWGLAAFVDGGNAWDTGDFDPAYGVGGGVRFRTPIGPVRADVAYGL
                     DVESWRLHFSVGFVF"
     gene            3127..>3678
                     /locus_tag="VFX94_00610"
     CDS             3127..>3678
                     /locus_tag="VFX94_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766707.1"
                     /translation="MLRWLVRGLVFILLLAAGGALWLLGTESGLRWALGFVPADIIIL
                     EEPRGTLLGTVSFERVAFQGSEARKVAFDLNLLALFADTISVEFVRIDSLTLRRFETK
                     EASEPSAGLPFRVRVADAQVKSVVFEGYEIHDVSAEYSGERSGHKVQAAFSGAGARAS
                     LKGSFNEEISLAARIQGLNVAVIH"
BASE COUNT          543 a         1231 c         1296 g          608 t
ORIGIN      
        1 tagcgggacg ctgcgtcttt gggaagtcct cgcgctgcgc ggcgaaaccg cgcgcgtgct
       61 gcccgatccg gtgcgcagcc tctgcgtcct cgatgcagcg ctcgaggctg gcctacgcct
      121 cattcacggc aagcggatgg atgccgagct tcaccccgca cgcgtgcggc gactgggcgc
      181 tgccggcgcc gaaatcgagt cgaaggcgcc agtcgcggtg ttcggcgcgc tccagctcgt
      241 tcttgcgatg gagccggctc ttgcgctcga cggcaaagtg atcgagctca ccgagcgcga
      301 tgggattgcg acggcgctcg tccggttcac cggcctcgac tgggacaccc gcgcgcgaat
      361 cgaaacctac gcccgggagc gaagggcgcg aggttatgat cccggctcca agccatgagc
      421 ccaatcattc cggcgctcgt cgcgctgccc gcctttgccg cgacccaggc gcaggcgctg
      481 gatcccgcgc cgcgcggccg caccaatgag ctcagcctga acgtgcaggt tgtcggctcg
      541 gacgattacg aattcgacgg cggcgcgagc gtgcgcaatg acggcggcgc aggcgtggcc
      601 ctctcgatcg cgcgcaacct gaacgatcat ttcgccatcg gcgcggaggt ggcctggtcg
      661 acctcgaatt ttcgggcgcg cgtcgcgccc ggggcgggaa attctggcgg gcgtttcgac
      721 tcggaaggcg acaccgagag cgtcgcgttg cgcctgcacg cgacctggta cctgctctcc
      781 cgccccatca cccctttcgt gaccgccggc gtgggggtga tctttttcga cgcggagctc
      841 gacgatagcc cccccgcgaa cacctgctgg atctacccgt ggtatgggga ggtgtgcgga
      901 gcaagcgcgc ccgagaacac gcttacgcgg ctcagctaca ccgccggcgc cggtctgcgg
      961 tgggatctac cgcccacttg gggacaccgc gagggcttcc tgcggctgct cctcggcggt
     1021 gaatggatcg acttcgaaga agcgtccagc cccgtcgggt actggcaggt gcgcctggat
     1081 ttcggagcgc ggttctagcg cttcgcgctg tagcccttct cccgcatgca cttctggaag
     1141 gactcgacct cgctgcgctc gcgctgctcc tcgagcgtca gcacccgggt cgtctgcgtt
     1201 ccggtctgcc cgtacagggc cggctggggc gagttcgaac gcgcgttggc gaggcactga
     1261 tcgttgtctt gctgcaccat ctcggcggtg gcgccgggct tttcccagta cgtcgagggg
     1321 gccgcgcagg cggcaagggc gagggcgagg atcaaggcat gacgggcggg gttcatgccg
     1381 tctattagag catggctgtg cctgacgttg ctcgtgtgcg gcgcagccct ggcgcaggag
     1441 cctgcaccga ccaagtaccg cctgagggtc gaggcgccaa cgaaggagct gcgcgagctg
     1501 ctcggcaagg ggctgcagct cgcgcgctgg caggacgacc cgcagatgac gccggagctg
     1561 ctgcgccggc ttgccgacga ggccgttgtc gaagcgcggc aggcgctcgc cgcggacggc
     1621 tacttcgcgg cgctggtgag ctattcaatc gaccgcgacg tcagcccgtg gtcggtggtg
     1681 cttcacgtcg agccggggac gcgcgccacg gtaagcggcg tagagatcac gttcaccggg
     1741 ccggcgagca ccgaccccga agcggcgaag cacctggcgc gcgtgcgcaa cgaatggctc
     1801 ctgcggcccg gcatggcgtt cacccaggaa gcgtggggcg aggcgaaacg cgaagcggtg
     1861 cgcaggcttt ccgcgtggcg ctacgcggag gcgcgcatcg cttccagccg cgcgcgcgtg
     1921 gacgcgcagg cgaacacggc gatgctcgag atcaccctgg agagcggccg gccgttccgc
     1981 ttcgggggcg tggaagtgcg cggcaacgga cgctacgaca actcgatcgc cgcgaacctg
     2041 agcccggtcg agcccggcac gacgtacgac cgcgaggtgc tggagctcta tacccgccgc
     2101 ctgctcgagc cgggctactt cgccggcgcg cgcgccgagc tcgcgcccga ggcggcgacc
     2161 gacggccagg cgccggtcaa cgtttcggtg atcgagggac actcgcagaa catcgaaggc
     2221 ggcgtgtcgt tcaccaccga ccgcggcgtg cgcctggagg ctgcgcatcg caacgccgac
     2281 ctcctcgact cggcgtggcg cgggcgcgcc tcggcgcggt gggacgagct cacgcaggaa
     2341 gcgcgctacg acctcgacgc gccgccgctt cccggcgcgc gctggtggag cggcttcacc
     2401 gcggtgaaga acgagttgat ccagaacgag gagaacttcg agttctccgg cggcatcgcg
     2461 cacaactggg cgggaaaggg ctcgccgatc tcgatcttcg cctccggcca cctcgagaac
     2521 cagcaggtga gcggccagga agtcgacaac cgccaggcgg tgttcttcgg cttccgcgtc
     2581 ggcttccgag agaccgacgc ggtcgttctg ccgcggcgcg gctacttcgg ccagttcacc
     2641 ggcggcgtgg cgcccgaaaa tctttccacg cagcacttcg tgcgcggcac gtggcgcgtt
     2701 acgttcctcg tgccgctcgg gcgcaacgac gatctgagca tgcgcgccga ggcaggcctc
     2761 gtggtcgcca actctcgcgc gggaattccg tccactttcc tcttccgtac cggcggcgac
     2821 cagaccgtgc gcggctatgc cttcgaaagc ctcggcgtgc gcgaagggga cgcggtcctc
     2881 ggcgggcgct acatggcgat cggcagcgtg gaatacactc actggttctc gcccgtgtgg
     2941 gggctcgccg cattcgtcga cggcggcaat gcctgggaca ccggcgactt cgacccggcg
     3001 tacggcgtgg gcgggggcgt gcgcttccgc actccgatcg ggccggtgcg cgccgacgtc
     3061 gcctacggcc tggacgtgga aagctggcgg ctgcacttct ccgtaggttt cgtgttctga
     3121 ttcggcatgc tgcggtggct cgtccgcgga ttggttttca tcctgctgct tgcggccggc
     3181 ggcgcgctct ggctgctcgg gaccgagagc gggctgcgct gggcgctcgg cttcgtgccg
     3241 gccgacatca tcattctgga agagccgcgc ggcacgctgc tcggcacggt ctccttcgag
     3301 cgcgtcgcgt tccagggaag cgaggcgcgc aaggtggcct tcgacctgaa cctccttgcg
     3361 ctcttcgccg acaccatctc ggtggaattc gtccgcatcg acagcctcac tctgcggaga
     3421 ttcgagacca aagaagcatc cgaaccgtcg gctggcttgc cgtttcgcgt ccgtgtcgcc
     3481 gacgcccagg tgaagtcggt ggtgttcgag ggctacgaga tccacgacgt aagcgccgag
     3541 tattcggggg agcgctccgg ccacaaggtg caggccgcgt tctccggcgc cggcgcgcgt
     3601 gccagcctga aagggtcttt caacgaggag atctcgcttg cagcaagaat ccaagggctc
     3661 aacgtggcgg tcattcac
//
LOCUS       DASUNT010000032         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_442776, whole genome shotgun sequence.
ACCESSION   DASUNT010000032 DASUNT010000000
VERSION     DASUNT010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442776"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFX94_00615"
     CDS             complement(<1..205)
                     /locus_tag="VFX94_00615"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX5766708.1"
                     /translation="MKFWYRRGAIAWLLWPASFAFRLVVLGRKLLYRLGVLRSAHPGI
                     PVIVVGNLTVGGSGKTPLVIWVAQ"
     gene            complement(206..622)
                     /locus_tag="VFX94_00620"
     CDS             complement(206..622)
                     /locus_tag="VFX94_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX5766709.1"
                     /translation="MNFRRGQEKEPLEINLVPMIDVMLVILIFLMITTTYSKYTELQI
                     NLPAARADRQLERPNEVSVLVNAQGQYVVNRRPVPFRSVEQLADELRRVGAPMQEPVV
                     VISADANATHQAVIRVMEASRLAGLAQITFTTQSRK"
     gene            complement(619..1230)
                     /locus_tag="VFX94_00625"
     CDS             complement(619..1230)
                     /locus_tag="VFX94_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX5766710.1"
                     /translation="MFAIVQAAGWPIWFLLFASVIAVALIIERSVTLRASRIIPPMLL
                     DQVVSVVQRQGLSAEVIERLSRDSPLGTVLAAGLRNLKSSRYVMKEAIEEAGRGVAHE
                     LGRFLTTLGTIATAAPLLGLFGTVIGMIEIFGSQSPTGGTNPQQLAHGISIALYNTAF
                     GIALGIAALVFYRHFRARVDTFVVEMEQLAAKLVDLVHGERAA"
     gene            1603..>2295
                     /gene="xseA"
                     /locus_tag="VFX94_00630"
     CDS             1603..>2295
                     /gene="xseA"
                     /locus_tag="VFX94_00630"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX5766711.1"
                     /translation="MQNDIPGAAVASALRIVSVAELLRSVRDLVERRFALTWVRGELS
                     NLSRAPSGHLYFTLKDRAAQVDCVMFRSRAVALDWEPRDGVQVEVRALATLYEPRGRF
                     QLTVEAMRRAGLGPLYERFLRLKENLGAEGLFEERLKRALPAHPRRIGVVTSLAAAAL
                     RDVLTTLARRNPAIEVVVYPVPVQGEGAAERIATMLARASRRAECDVLLLVRGGGSIE
                     DLWQFNEEALARA"
BASE COUNT          394 a          735 c          760 g          406 t
ORIGIN      
        1 attgcgcgac ccagatgacg agcggcgtct tgccgctgcc gccgacagtc aggttgccaa
       61 ccacgatcac cgggattccc ggatgcgcgc tcctcaggac gccgagccgg tagaggagtt
      121 ttctaccaag aaccacgagc cgaaaagcga agctcgccgg ccacagcagc caggcgatgg
      181 cgccgcggcg gtaccagaac ttcacctact tccgcgactg cgtggtgaac gtgatctggg
      241 ccaggccggc gaggcgcgag gcctccatca cgcggattac cgcctggtgc gtcgcgttcg
      301 cgtcggcgct gatcaccacg accggctcct gcatgggagc gccgacgcgg cgcagctcgt
      361 cggcgagctg ctcgacggag cgaaacggca ccggcctgcg gttcacgacg tactggccct
      421 gcgcgttgac gagcacgctc acttcgttcg gccgttcgag ctgtcgatcc gcgcgcgccg
      481 cgggcaggtt gatctgcagc tcggtgtact tcgagtacgt ggtagtgatc atcaggaaga
      541 tcaggatgac cagcatcacg tcgatcatcg gcacgaggtt gatctcgagc ggctccttct
      601 cctgcccgcg ccggaagttc acgccgcgcg ctctccgtgc accaggtcca cgagcttcgc
      661 cgcgagctgc tccatctcga ccacgaaagt gtcgacgcgg gcccggaagt gccggtagaa
      721 cacaagcgcc gcgatgccga gcgcgatgcc gaacgcggtg ttgtagaggg cgatggagat
      781 gccgtgcgcg agctgctggg ggtttgtgcc gccggtgggc gactgcgagc cgaagatctc
      841 gatcatgccg atcaccgtgc cgaaaagccc gaggagcggc gccgccgtgg cgatcgtgcc
      901 gagcgtagtg aggaagcgcc cgagctcgtg cgccaccccg cgccccgcct cctcgatcgc
      961 ctccttcatc acgtagcgcg agctcttcag attgcgcagg cccgcggcga gcaccgttcc
     1021 gagcggcgag tcgcgcgaca gccgctcgat tacctccgcc gacagccctt ggcgctggac
     1081 caccgacacc acctggtcaa gcagcattgg cggaatgatg cggctcgccc ttaacgtgac
     1141 ggagcgctcg atgatcaggg ctactgcgat gacagaagcg aagaggagga accaaatggg
     1201 ccagccagcg gcctggacga ttgcgaacaa cgcctatcct tatcgttatt ctggatgcgt
     1261 ggccgccgac tgtaagccaa agcctctcga acgggcaagt ttcgcgctgc agcaaagcgg
     1321 ctgcgcagac tattgtgtga attgtcacac gcacctgtag tgctttttgc gatatccaca
     1381 aaaaactgtg gataagtttg tggatggctg aggaatttgt tgtcacaagc ccgttgcgat
     1441 ggcgtcgaag tcggtggcga tcaaaatttg agcagaaatt ttcttcggag ccacaacccg
     1501 ttacgtaaaa gaatcgggtt ccgcgaggag ttcggtcgcg gttgcgcggc actgcggcgg
     1561 gaatgtggat tagtgtaggc tcggctgcgc ctcctgacat cgatgcagaa cgatattcca
     1621 ggcgccgctg tcgcctccgc cctacgcatt gtctcggtcg cggaattgct gcgcagcgtg
     1681 cgcgatctgg tcgagcggcg ctttgcgctc acctgggtgc gcggcgagct ttccaacctt
     1741 tcgcgcgctc cctccggtca tctttacttc acgctgaagg accgcgcggc gcaggtcgac
     1801 tgcgtgatgt tccgtagccg ggccgtggcg ctcgactggg agccgcgcga tggcgtgcag
     1861 gtcgaggtcc gggcgctcgc cacgctctac gagccgcgcg ggcgtttcca gctcacggtg
     1921 gaggcaatgc gccgagcggg gctcggcccg ctctacgagc gtttcctgcg cctcaaggag
     1981 aatctcggcg ccgaaggact gttcgaggag cggctgaagc gtgcgctgcc cgcgcacccg
     2041 cgccgcatcg gagtcgtcac ttcgctcgct gcggctgccc tgcgcgacgt cctgaccacg
     2101 ctcgcgcgtc gcaacccggc gatcgaggtc gtggtctatc cggtgccggt gcagggcgaa
     2161 ggcgcggccg agcgcatcgc gaccatgctg gcacgcgcga gccgccgcgc cgaatgcgac
     2221 gttctgctcc tcgtacgcgg cggcggctcc atcgaggatc tgtggcagtt caatgaagaa
     2281 gcgctggcgc gcgcc
//
LOCUS       DASUNT010000033         3280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_793509, whole genome shotgun sequence.
ACCESSION   DASUNT010000033 DASUNT010000000
VERSION     DASUNT010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793509"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VFX94_00635"
     CDS             complement(<1..358)
                     /locus_tag="VFX94_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEX5766712.1"
                     /translation="MTVTQDLDIWTLILHASMVVKVVMGLLVLVSFMSWMFIFRKWMT
                     IRSARAQTEQFEREFWSGNDINSLYQGSVNNRHSIGSLERIFEAGYREFTKLRAQRGT
                     DPSTMVDGARRAMRATY"
     gene            complement(355..732)
                     /gene="ybgC"
                     /locus_tag="VFX94_00640"
     CDS             complement(355..732)
                     /gene="ybgC"
                     /locus_tag="VFX94_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEX5766713.1"
                     /translation="MRVYYQDTDAGGVVFHGQYLAFMERARSELLDQAGIDLARFVER
                     HGVLFMVHRIEVKYHLPARLNELLAVSAEVVKMGRASFVFHQRVERAAELLVEAEVAI
                     ALVDRERMKPARMPAELEQALST"
     gene            complement(741..1778)
                     /gene="ruvB"
                     /locus_tag="VFX94_00645"
     CDS             complement(741..1778)
                     /gene="ruvB"
                     /locus_tag="VFX94_00645"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX5766714.1"
                     /translation="MTVEPDRMISARPALPQESEFERSLRPATLAEYVGQDKVRSQLE
                     IFIAAARGRGEALDHVLLFGPPGLGKTTLAHIIARELGVNLRQTSGPVLERPGDLAAI
                     LTNLEPKDVLFIDEIHRLAPVVEEILYPALEDFQIDIMIGEGPAARSIKLDLPPFTLA
                     GATTRAGMLTNPLRDRFGIVARLEFYSEQDLARIVQRSARLLKVSLESDGAAEIARRA
                     RGTPRVANRLLRRVRDYAQVKGDGRVSKQVAEAALKMLDVDVLGLDLMDRKLLLAVIE
                     KFAGGPVGVENLAHAVGEEADTIEDVLEPFLIQQGYLQRTPRGRIATLLAYRHFGIVA
                     PAAAKDLLSDA"
     gene            complement(1890..2450)
                     /locus_tag="VFX94_00650"
     CDS             complement(1890..2450)
                     /locus_tag="VFX94_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX5766715.1"
                     /translation="MKKRTWLAGLGLAAALAAAPAAAQLYAGGSVGYTQYKDICDVVA
                     PGVTCDDNDTGFRVFGGYQFNQHFALELGFADLGESSGSGAPGNFIVEAKEAFDLSMV
                     FSLPVANRLSLLFRLGAHRTRTTVEDTGLEEAKTGSAFTLGAGAEYSFGKLGIRAEWQ
                     RYDNISGGTVAETDVDVFSIGALYRF"
     gene            complement(2611..3192)
                     /locus_tag="VFX94_00655"
     CDS             complement(2611..3192)
                     /locus_tag="VFX94_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX5766716.1"
                     /translation="MTKGLAILGLAFAMSFTGPALAQDKGFFIGASIGQTDVDEEITS
                     GVIDPGGNVDGEDTAWKIYGGYMFNRHFGVELSYIDAGEVSYRGTFAGLPVTEGKVEL
                     SAWNVSALGVLPINEQFSIFGKVGLFKWEAQYSDTTGGIPFSADDDGTDASFGVGLAW
                     NFTRNLGLRAEYEFFKTDEADVNLLSVGVVWKF"
BASE COUNT          570 a         1092 c         1050 g          568 t
ORIGIN      
        1 ggtaggtcgc gcgcatggcg cggcgcgcgc cgtcgaccat ggtcgaggga tccgtgccgc
       61 gctgcgcgcg cagcttcgtg aactcgcggt agccggcctc gaagatgcgc tccaggctgc
      121 cgatcgagtg gcggttgttg accgagccct ggtagagcga attgatgtcg ttgccgctcc
      181 agaactcgcg ctcgaactgc tcggtctggg cgcgcgcgct gcggatcgtc atccacttgc
      241 ggaagatgaa catccacgac atgaacgaca ccagcacgag cagccccatg accaccttca
      301 ccaccatgct tgcgtggagg atcagcgtcc agatgtcgag gtcctgggtg acggtcatgt
      361 cgacaacgcc tgttcgagtt ctgcgggcat gcgcgcgggc ttcatccgct cccggtcgac
      421 cagcgcaatg gcgacctccg cttcgaccag gagctcggcg gcgcgttcca cgcgctggtg
      481 gaacacgaag ctcgcgcggc ccattttaac cacttcggcg ctcaccgcta ggagctcgtt
      541 gagacgcgcc ggcaggtgat atttcacctc gatgcgatgg accatgaaga gcacgccatg
      601 cctttcgacg aagcgcgcga ggtcgatgcc cgcctggtcg aggagctcgc tgcgcgcgcg
      661 ctccatgaag gcgaggtact ggccgtggaa cacgacaccg cccgcgtcgg tgtcctggta
      721 gtagacccgc acgggaatcc tcaggcgtcg gacagcaggt cctttgccgc cgcgggggcc
      781 acgatgccga agtggcgata cgcgagcagc gtggcgatgc gcccgcgcgg agtgcgctgc
      841 aggtagccct gctggatgag gaagggctcg agcacgtcct cgatcgtgtc ggcctcctcg
      901 ccgacggcgt gcgcgaggtt ttccacgccg accgggccgc cggcgaactt ctcgatcacc
      961 gcgagaagca gcttgcgatc catcaggtcg agcccgagca catcgacgtc gagcatcttg
     1021 agcgccgcct ccgccacctg cttcgaaacc ctgccgtcgc ccttcacctg ggcgtaatcg
     1081 cgcacacggc gcaggagccg gttcgccact cgcggggtac cgcgcgccct gcgcgcaatc
     1141 tccgccgcgc cgtcgctctc gagcgagacc ttgagcaggc gcgcggagcg ctgcacgatc
     1201 ctggcgaggt cctgctcgct gtagaactcg aggcgcgcga cgatgccgaa gcggtcgcgc
     1261 agcgggttgg tcagcatgcc ggcgcgcgtg gtggcgccgg cgagcgtgaa cggcggcaag
     1321 tcgagcttga tcgagcgcgc cgccgggccc tcgccgatca tgatgtcgat ctggaagtcc
     1381 tcgagcgccg gatagaggat ctcctccacc accggcgcaa ggcggtggat ctcgtcgatg
     1441 aaaaggacgt ctttcggctc gaggttggtg aggatggcgg cgaggtcgcc gggccgctcg
     1501 agcaccggcc cggaggtctg gcgcaggttc acaccgagct cgcgcgcgat gatgtgcgcg
     1561 agcgtggtct ttcccaggcc gggcgggccg aagagcagca cgtgatcgag cgcttcaccg
     1621 cggccgcgcg ccgccgcgat gaagatctcg agctgcgagc gtaccttgtc ctggccgacg
     1681 tattcggcga gcgtcgccgg cctgagcgag cgctcgaact cgctttcctg cggcaaggcg
     1741 ggccgcgccg agatcatgcg gtcgggttcg acagtcataa gtggcccgga acccgcctca
     1801 acacgcgggg cctggtttcc ggccctcgat tctgccagag ctcctatgac gctgccccgg
     1861 cgcccgcctg cccgccaaac cccggtgcgt cagaacctgt agagtgcccc gatgctgaag
     1921 acgtccacgt ccgtctcggc cacggttccg ccggagatat tgtcgtagcg ctgccattcc
     1981 gcgcggatgc cgagcttgcc gaacgaatac tccgcgccgg cgcccagggt gaacgccgag
     2041 ccggtctttg cctcctccag gcctgtgtcc tcgacggtgg tgcgggtgcg atgtgcgccc
     2101 agcctgaaaa gcagcgacaa gcggttcgcc accgggaggg agaacaccat gctcagatcg
     2161 aatgcttcct tcgcctcgac gatgaaattt cccggggcgc cggagccgct cgactcgccg
     2221 aggtccgcga acccgagctc gagcgcgaag tgctggttga actggtagcc gccgaacacg
     2281 cgaaaacccg tgtcattgtc atcgcaggtg acacccggcg cgaccacgtc gcagatgtct
     2341 ttgtactggg tatagccgac cgagccgccg gcgtagagtt gtgctgccgc tggagcggcg
     2401 gccagcgccg cagcgagtcc caggccggcc agccaagttc tttttttcat gccaaacccc
     2461 cgttattgtt gtaccgacaa ccgcgcacac tacaacgact tttccgaacg gccaaattcg
     2521 atcacgagca gtgctgacgt ggccggtcga gccggcaaga aaaagggcgg ccaaagccgc
     2581 ccttccgcac gccaggctgc aacgacgcgc tcagaatttc cagaccacgc cgaccgacag
     2641 caggtttacg tccgcctcgt cagtcttgaa aaactcgtac tccgcccgca ggccgagatt
     2701 gcgggtgaag ttccacgcga ggccaacccc gaacgaggcg tcggtgccgt catcatcggc
     2761 ggaaaacggt atgccgccgg tggtatcgga gtactgggcc tcccatttga acagtccgac
     2821 cttgccgaaa atcgagaatt gctcgttgat cggcagcacg ccgagcgccg agacgttcca
     2881 tgcgctcagc tccaccttgc cttcggtcac gggaagcccg gcgaacgtac cgcggtagct
     2941 cacctcgccg gcatcgatgt aggaaagctc tacgccgaaa tggcggttga acatgtagcc
     3001 gccgtagatt ttccaggcgg tgtcctcgcc atcgacgttg cctcccgggt cgatcacgcc
     3061 gctcgtgatt tcctcatcga cgtcggtctg cccgatgctc gccccaataa agaagccttt
     3121 gtcctgcgcc aacgccgggc ccgtgaagct catggcgaac gcaagaccca ggatggccag
     3181 tccctttgtc actttcataa tcttttccac tcctgttgtt gaatgattga acgcatcacg
     3241 cgcgctttgc gttcggcgca gacactacac tgagcacggc
//
LOCUS       DASUNT010000034         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_92198, whole genome shotgun sequence.
ACCESSION   DASUNT010000034 DASUNT010000000
VERSION     DASUNT010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92198"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="VFX94_00660"
     CDS             <1..1043
                     /locus_tag="VFX94_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX5766717.1"
                     /translation="TLLDALVLLALTLGGGLQWLSDLWRAFFAVESMWHGMALILSLL
                     LLKGALGLPLSVYRIFVVEQRFGFNRMTWKLFIADLAKGLAVGAVLGVPLLLAVLWLA
                     LAAGELWWLYVWLVLVVYSLFLQMIYPAVVMPLFNKFSRLEDPALAGRIEQLLSRTGF
                     RSRGLYVMDGSKRSSHGNAFFAGFGAAKRIVLFDTLVSRLRPAEVEAVLAHELGHYKL
                     RHIVKAMALSWAFSFLLLLALGFLAGQPWFYRGLGMETATLPIGLALFMLVTPEFTFF
                     VQPLLSLFSRKNEYEADRYAAANADAAELVRALVKLYHDNSATLTPDPLHSAYYDSHP
                     PAAMRIARLRTT"
     gene            1040..1369
                     /locus_tag="VFX94_00665"
     CDS             1040..1369
                     /locus_tag="VFX94_00665"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX5766718.1"
                     /translation="MNPLASRRCQPCEGGVKPFTPQEANELLKTLKGWAIENGRLART
                     FAFQNYYETMAFVNALAWISHREDHHPDLGVHFNKCRVEYWTHAIGGLSENDFICAAK
                     ADALFEL"
     gene            1366..1662
                     /locus_tag="VFX94_00670"
     CDS             1366..1662
                     /locus_tag="VFX94_00670"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX5766719.1"
                     /translation="MKRVYSSFNQTAVYHARNLLENAGIEVLVRNAILSSAMGELPPA
                     ECQAELWVLDDSDAGRAEELLFQPVTGEDWDCACGEKLGAQFTQCWRCGACRAG"
     gene            complement(1670..>2036)
                     /locus_tag="VFX94_00675"
     CDS             complement(1670..>2036)
                     /locus_tag="VFX94_00675"
                     /inference="COORDINATES: protein motif:HMM:NF015164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEX5766720.1"
                     /translation="TFAGQTFHLLEWPAVRLTAAAFALVGDFENTVYCWRTQARSWPD
                     PDAGVLLAAGAGAMGVRLGMPVQEVDGVQPRPELGVGEQADGPFLDTTVGMLWRALVL
                     WVAVLVGAALVAYGSRLWA"
BASE COUNT          306 a          705 c          653 g          372 t
ORIGIN      
        1 acacgctcct cgatgcgctc gtccttctcg ccctgactct cggcgggggc ctgcagtggc
       61 tctcggacct gtggcgcgcc tttttcgcgg tcgaatcgat gtggcacggg atggcgctca
      121 tcctgtcgtt gctgctcctc aagggcgcgc tcggtctgcc gctcagcgtc taccgcattt
      181 tcgtcgtcga acagcgcttc ggcttcaatc gcatgacgtg gaagctcttc atcgccgatc
      241 tcgccaaggg tctcgcggtc ggcgcggtgc tcggcgtgcc gctgctcctt gcggtgctgt
      301 ggctcgcgct cgcggccggc gagctgtggt ggctgtacgt ctggctcgtg ctcgtcgtgt
      361 actcgctttt cctgcagatg atctaccccg cggtggtcat gcctctcttc aacaagttct
      421 cccggctgga ggacccggcg cttgccggca ggatcgagca gctcctcagc cgcacgggtt
      481 ttcgctcgcg cggcctctac gtgatggatg ggtcgaaacg ctcctcgcac ggcaatgcgt
      541 tcttcgccgg cttcggcgcc gcgaagcgaa tcgtcctttt cgatacgctg gtgtcgcgcc
      601 tgcggccggc cgaggtcgag gcggtactcg cacacgagct tggccactac aagctgcgcc
      661 acatcgtgaa agccatggcg ctcagctggg cgttcagctt cctgctgctc ctcgcgctgg
      721 gcttcctcgc cgggcagccg tggttctacc gcgggctcgg catggagacc gccacgctgc
      781 cgatcggcct cgcgcttttc atgctcgtca cgcccgagtt cacgttcttc gtgcagccgc
      841 tgctttcgct tttctcgcgc aagaacgagt acgaagccga ccgctacgcc gcggccaacg
      901 ccgacgcggc ggagctggtg cgcgcgctgg tgaagctcta ccatgacaat tcggcgacgc
      961 tgacccccga cccgctgcac tcggcctact acgactcgca tccgccggcg gcgatgcgca
     1021 tcgcccgctt gaggaccaca tgaacccgct cgccagtcga cgctgccagc cctgcgaagg
     1081 cggcgtgaag ccgttcacgc cgcaggaagc gaacgagctg ctgaagaccc tcaagggctg
     1141 ggccatcgag aacggcaggc tcgccagaac tttcgcgttc cagaactact acgagaccat
     1201 ggccttcgtg aacgcgcttg cctggatctc gcaccgtgag gaccaccatc ccgacctcgg
     1261 cgtgcacttc aacaagtgcc gcgtcgaata ctggacccac gcgatcggcg gactgtcgga
     1321 gaacgacttc atctgcgcgg cgaaggccga cgcgctgttc gagctttgaa aagggtctac
     1381 agctccttca accagacggc cgtctatcat gcgcgcaatc tcctggagaa tgcgggcatc
     1441 gaagttctcg tgagaaacgc gatcctgtcc tccgccatgg gcgagctgcc gcccgccgaa
     1501 tgccaggcgg agctttgggt gctcgatgat tccgacgcgg gccgggcgga ggagctcctt
     1561 tttcagccgg tgaccggcga ggactgggac tgcgcctgcg gcgagaagct tggcgctcag
     1621 ttcacgcaat gctggcgttg cggcgcctgt cgcgccggct gaaagctgct caagcccaga
     1681 ggcgcgagcc gtaggcgacg agcgcggcgc ccacgagcac ggcgacccag aggacgagcg
     1741 cccgccacag catcccgacc gtcgtgtcga ggaacgggcc gtcggcttgc tccccgacgc
     1801 cgagctcggg tcgcggctgc acgccgtcga cttcctgcac cggcatgccg agccgcactc
     1861 ccatggcgcc cgcccccgcg gcgagcagca cccctgcgtc cggatcgggc cagctgcgcg
     1921 cctgggtgcg ccagcagtac acggtgttct cgaagtcgcc gaccagcgcg aatgccgccg
     1981 cggtgaggcg caccgcgggc cattcgagaa ggtgaaacgt ctgcccggcg aaggtg
//
LOCUS       DASUNT010000035         5811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1056849, whole genome shotgun sequence.
ACCESSION   DASUNT010000035 DASUNT010000000
VERSION     DASUNT010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056849"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VFX94_00680"
     CDS             <1..973
                     /locus_tag="VFX94_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766721.1"
                     /translation="TGDFDGARVAIKQAHELEAQIAEARARQYAEVEESARTRGARTS
                     FKELNGYPIETIDTPEVKALRNGYQSALSHYVAGFIYEALGEPSLAAPGYRLANELQP
                     GQPLLEDALRGLDERVAGAHNDGTTDVLFIVGSGSAPAIQSREFLLPVFVDNRMLLIA
                     NAFPVMKTTSWTSVPSEVTLDGQTYPAARITSIGLMARRRLMDDMPAILLRATIRSTL
                     SATLQYQAQRAADREHSAAMAIAAGIITTGSVLLASADDRTWRALPSDVSIARARVPR
                     GVHTVTLHTAEGARSVSVAVSGRYAVVDLRLLRYQLFVSAPTLSAER"
     gene            989..1423
                     /locus_tag="VFX94_00685"
     CDS             989..1423
                     /locus_tag="VFX94_00685"
                     /inference="COORDINATES: protein motif:HMM:NF018889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HEX5766722.1"
                     /translation="MKPALLIAAIAAAALAAGCGTKKGFEKPGYAFGCPIGETKVSSM
                     DDLVKAKVQSEGRRTTIQATEMRCSQAGDLMRIDANLNNDSRSVKRIAYKFRWIDREG
                     MRAWEEEPWKPLMLYENSNLVINTISPSNKAVDFRLVLRSEE"
     gene            1420..1992
                     /gene="lpoB"
                     /locus_tag="VFX94_00690"
     CDS             1420..1992
                     /gene="lpoB"
                     /locus_tag="VFX94_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HEX5766723.1"
                     /translation="MNLRILLALAAAAIVGGCAKEQTRYGDARGIETVTNQYGSTDLQ
                     MMAESMARSMLQAPVIASGNLPVVTVQEVRNKTSEYIDTRAITDSIRAELQKGGRVRF
                     AVDQAGMNQQVDELKRQQGGLYAKETAAEMGQMVGAQYRLEGNIISIVKQVRETKDVY
                     YKLNLQLWNVRNGLLEWSDEKEIRKTTTKG"
     gene            1997..2173
                     /locus_tag="VFX94_00695"
     CDS             1997..2173
                     /locus_tag="VFX94_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766724.1"
                     /translation="MKRKYGAALARETLAEQRMRERAAEMHDNSKNFGSQSKMGSPTR
                     GKRRGRAYKPAHRR"
     gene            complement(2244..2450)
                     /locus_tag="VFX94_00700"
     CDS             complement(2244..2450)
                     /locus_tag="VFX94_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766725.1"
                     /translation="MAPDTPNLTALLNMAPSAFASGDMLPLPRIKSISHLRKQVERHF
                     GKLSSQDTEALVGWFNRNFFRLEK"
     gene            complement(2607..4589)
                     /locus_tag="VFX94_00705"
     CDS             complement(2607..4589)
                     /locus_tag="VFX94_00705"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEX5766726.1"
                     /translation="MKDFFNLYRHGFARVAVATPVVRVADPQYNLVATLELMREAARE
                     KALLAVFPELGLSAYSCEDLFHQHALVASAEDALSELVRKTKSLPLAALVGLPVAVDG
                     LLYNCAALVCRGRLAGVVPKTYLPNYREFYEGRQFTPGDAARCKAIRLAGQHAPFGTD
                     LLFRFTGLKGFVLHVEICEDLWVPTPPSSFAALAGATLIANLSASNVVIGKEGYRRQL
                     VGNQSARCLAAYLYSAAGLGESTTDLAWDGHALIYENGTLVGESRRFADAPQLALADV
                     DLDRLLADRMRQNTFGAAAVRNTHEVAKFRSIEIDLQAPGGRLLLERKIERLPYVPSD
                     PRTRDARCEEVYRIQVQGLVTRMRATGSKRLVIGVSGGLDSTQALIVCARAMDELKLP
                     RANILAYTMPGFATSTRTKSQAWQLMRAVGATAEEIDIRPSCLQMLKDIGHPFARGKK
                     LYDITFENVQAGERTSHLFRLANLHGGMVVGTGDLSELALGWATYGVGDHMSHYNVNA
                     SVPKTLIQFLIGWVSESAEFGADVKRALRRVLETEISPELVPGRQSSEASVGPYELQD
                     FNLYYILRFGYSPAKVAFLAWHAWRGKYRLPAIRKWLGVFLKRFFQNQFKRSAIPNAP
                     KVGSGGSLSPRGDWRMPSDASAEAWLRNLKEVPADG"
     gene            4632..4811
                     /locus_tag="VFX94_00710"
     CDS             4632..4811
                     /locus_tag="VFX94_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766727.1"
                     /translation="MLLLRETVVTRDGPLEIELHDDLRYRLSWQGVVYDQKRRYDFRS
                     VEQLRYDFERDVEKA"
     gene            4795..5148
                     /locus_tag="VFX94_00715"
     CDS             4795..5148
                     /locus_tag="VFX94_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5766728.1"
                     /translation="MSRKLEIRVGAAGDALDRFEAAWNRVREGRAMRPLEILSFEDLP
                     LLTRALTPARWTLLQDLKRKGPCSIYELARRLGRDYKNVHTDVTQLAAIGLLERRADA
                     LASVPWDLLRAELKL"
     gene            5206..>5811
                     /locus_tag="VFX94_00720"
     CDS             5206..>5811
                     /locus_tag="VFX94_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006415791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5766729.1"
                     /translation="MNRDLSPTSALNLEPYWMPFTDNRRFKAKPKLVTAAKDMHFTTA
                     EGRQVLDGLATLWCVNAGHARPRIVEAIRRQAGELDFAASFSLGHPGAFRLAERIAAL
                     APAGMGHVFFTNSGSEAIDTAMKIAVAYHRLRGEGTRTRFVGRERSYHGVNLAGMSLG
                     GVPANRKAYSGVLLSGVDHLRHTHLAENAFSRGQPARGAELA"
BASE COUNT         1040 a         1936 c         1957 g          878 t
ORIGIN      
        1 gacgggcgac ttcgacggcg cacgcgtggc gatcaagcag gcgcacgagc tcgaggcgca
       61 gatcgccgag gcgagggcca ggcagtacgc cgaagtggag gagtcggcgc gcacgcgcgg
      121 cgcgcgcacc agcttcaagg agctgaacgg ttacccgatc gagaccatcg acacgccgga
      181 ggtcaaggcg ctgcgcaacg gctaccagag cgcgctctcg cactatgtcg ccggattcat
      241 ctacgaggcg ctcggcgagc cgagcctagc cgcgccggga taccggctcg cgaacgagct
      301 gcagcccggc cagccgctgc tggaagatgc gctgcgcggg ctcgacgaac gcgtcgccgg
      361 tgcgcacaac gacgggacta ccgacgtgct gttcatcgtc ggcagcggat cggcgcccgc
      421 gatccagtcg cgcgaattcc tgctgccggt gttcgtcgac aaccgcatgc tgctcatcgc
      481 caacgccttc ccggtgatga agaccacctc gtggacttcc gtgccgagcg aggtgacgct
      541 cgacgggcaa acctatcctg cggcgcgcat cacgagcatc ggcctcatgg cgcgccggcg
      601 cctgatggac gacatgccgg ccatcctgct gcgcgccacg atccgctcga cgctcagcgc
      661 gacgctgcaa taccaggctc agcgcgccgc cgaccgcgag cactcggcgg cgatggcgat
      721 cgccgccggc atcatcacca cgggctcggt cctgctcgcc tccgccgacg accgcacctg
      781 gcgcgcgctg ccctccgacg tgtcgatcgc ccgggcgcgc gtgccgcgcg gtgtgcacac
      841 ggtcacgctg cacacggcgg agggcgcgcg cagcgtctcc gtcgccgttt ccggacgtta
      901 cgcggtggta gacttgcgcc tgctgcgcta ccagcttttc gtgagcgctc cgacgctgag
      961 cgcagagcgt tgaaacaggg aggtcaggat gaaacccgcg ctcctcatcg ccgccattgc
     1021 cgctgccgcg ctcgccgccg gctgcggcac aaagaagggc ttcgagaagc ctggctacgc
     1081 tttcggctgc ccgatcggcg aaaccaaggt gagctcgatg gacgacctcg tcaaggcgaa
     1141 ggtgcagagc gagggacggc gcaccaccat ccaggcgacc gagatgcgct gctcgcaggc
     1201 gggcgacctg atgcgcatcg acgccaacct gaacaacgac agccgcagcg tgaagcgcat
     1261 cgcgtacaag ttccgctgga tcgaccgcga aggcatgcgc gcctgggagg aggagccctg
     1321 gaagccgctc atgctctacg agaactccaa cctcgtcatc aacaccatct cgccgagcaa
     1381 caaggcggtc gatttccgcc tcgttctgag gagtgaagaa tgaatctccg gatcctgctc
     1441 gcccttgccg ctgccgcgat tgtcggcggc tgcgccaagg agcagacccg ctacggcgat
     1501 gcacgcggca tcgagaccgt cacgaaccag tacggctcca ccgacctgca gatgatggcg
     1561 gagtcgatgg cgcgctcgat gctccaggcg ccggtcatcg cgagcggcaa cctccccgta
     1621 gtcacggtgc aggaggtgcg caacaagacg agcgagtaca tcgacacccg cgccattacg
     1681 gacagcatcc gcgccgagct gcagaagggc ggccgggtgc gcttcgcagt ggaccaggcg
     1741 ggcatgaacc agcaggtgga cgagctcaag cgccagcagg gcggcctgta tgccaaggaa
     1801 accgccgccg agatgggcca gatggtcggc gcccagtacc ggctcgaagg caacatcatc
     1861 tcgatcgtca agcaggtcag ggagacgaag gacgtctatt acaagctgaa cctgcagctc
     1921 tggaacgtcc gcaacggcct gctggaatgg tccgacgaga aagaaatccg caagaccacc
     1981 acgaaaggct gattggatga agcgcaagta cggagccgcg ctcgcgcgcg agaccctcgc
     2041 cgagcagagg atgcgcgaac gcgcagcgga gatgcacgac aacagcaaga acttcggctc
     2101 gcagtcgaaa atgggctccc ccacgcgcgg caagcgccgc gggcgcgcct acaagccggc
     2161 ccatcgccgg taaaaaaaga gggccagacc ccgcgcagcg ggatccgacc ccgggaaacc
     2221 tgcttcctgc cccctggcgg tagctacttt tccagccgga agaagttcct gttgaaccag
     2281 ccgaccagcg cctcggtgtc ctggctcgag agcttgccga agtgccgctc gacttgcttc
     2341 ctgagatgcg agatcgactt gatgcgcggc agcggcagca tgtcgccgct cgcgaaggcg
     2401 ctcggggcca tgttcagcaa ggccgtgagg ttcggtgtgt ccggcgccat ggattcagcc
     2461 taacgcacgg cgcccgggcg ggatggcccg attcaatctt tgacagacgc ggaacgtgac
     2521 gccgtctaga aaaccgccgg cgaggcggca ataaggcggg aagcgccgcg cggcagcatt
     2581 gcgagccttt gtaagacggc tttcgctcag ccgtcggcag ggacttcctt caggtttcgc
     2641 aaccaggctt ccgcgctcgc atccgagggc atgcgccagt cgccgcgcgg ggagagcgag
     2701 ccgcccgagc ccaccttggg tgcgttcggg atcgcgctgc gcttgaactg gttctggaag
     2761 aagcgcttca ggaatacgcc cagccacttg cggatcgccg gcagccggta cttgccccgc
     2821 caggcgtgcc aggcgaggaa ggccaccttg gccggcgaat agccgaagcg caggatgtag
     2881 tagaggttga agtcctgcag ctcgtagggg cccacgctcg cctcgctcga ctgccgcccg
     2941 ggcacgagct ccgggctgat ctcggtctcg agcacgcggc gcagcgcgcg tttcacgtcg
     3001 gcgccaaact ccgccgactc agacacccaa ccgatcagaa actggatcag cgtcttcggg
     3061 acgctcgcgt tcacgttgta gtgcgacatg tggtcgccga ccccataggt cgcccagccg
     3121 agcgcgagct ccgaaaggtc tcccgtgccg acgaccatgc cgccgtgcag gttggcgagg
     3181 cgaaagaggt ggctcgtgcg ttcgccggcc tgcacgttct cgaaggtgat gtcgtagagc
     3241 ttcttgcccc tggcgaacgg gtgcccgatg tccttcagca tctgcaggca cgagggccgg
     3301 atgtcgatct cctcggccgt cgcgccgacc gcgcgcatga gctgccaggc ctggctcttc
     3361 gtgcgtgtcg aagtggcgaa ccccggcatg gtgtaggcga ggatattggc gcgcgggagc
     3421 ttgagctcgt ccatcgcgcg ggcgcagacg ataagcgcct gcgtcgagtc gagacccccg
     3481 gagacgccga tgacgaggcg cttgctgccc gtggcgcgca tgcgcgtgac cagcccctga
     3541 acctggatgc ggtacacctc ctcgcagcgt gcgtcgcgtg tgcgcgggtc gctcggcacg
     3601 tagggcagcc gctcgatctt gcgctccagc aggagcctgc ccccgggagc ttgcaggtcg
     3661 atctcgatac tgcggaattt tgctacttca tgggtattgc gtaccgcggc agcgccgaag
     3721 gtgttctggc gcattcggtc cgcaagcagg cgatcgaggt ccacgtcggc gagcgcgagc
     3781 tgcggcgcgt cggcgaagcg ccgcgactcg ccgaccagcg tgccgttctc gtagatgagc
     3841 gcatgcccgt cccaggcgag gtcagtcgtc gactcgccaa ggccggccgc gctatagagg
     3901 tacgcggcga ggcagcgcgc cgactggttg ccgaccagct ggcggcgata gccttccttg
     3961 ccgatcacca cgtttgacgc cgagaggttg gcgatgagcg tagcgccggc gagcgcagcg
     4021 aaggaggagg gcggggtggg gacccacagg tcctcgcaaa tttcgacgtg caggacaaat
     4081 cccttgagcc cggtaaagcg aaacagcaga tcagtcccga acggggcatg ctgccctgcc
     4141 agccggattg ccttgcagcg cgcggcatcc ccgggcgtga attgccggcc ttcgtagaac
     4201 tcgcggtagt tgggcaggta ggtcttcggc accacgccgg ccaggcgacc ccggcacacg
     4261 agcgccgcgc agttgtagag caggccgtcc accgcaaccg gcagcccgac cagcgctgcg
     4321 agcggaaggc tcttcgtctt tcgcacgagc tccgaaagag catcctccgc ggaggccacc
     4381 agcgcatgct gatggaagag atcctcgcag ctgtacgcgg aaagcccgag ctcggggaac
     4441 accgcgagca gggctttctc gcgcgccgct tcgcgcatga gctcgagcgt ggccacgagg
     4501 ttgtactgcg gatcggcgac gcgcaccacc ggcgtggcaa ccgcgacgcg cgcgaagccg
     4561 tggcgataca ggttgaagaa gtccttcacg cgctcatttt acgcgggctg gtatatggta
     4621 cgctaagcca tatgctgctc ctgcgcgaaa cggtagtcac gcgcgatgga ccgctcgaga
     4681 tcgagctgca cgacgacctg cgctaccggt taagctggca aggcgtcgtt tacgaccaga
     4741 agcggcgcta cgacttcagg tcggtcgagc agctgcgcta cgacttcgag cgcgatgtcg
     4801 agaaagcttg agatccgggt cggcgcggcg ggcgacgcgc tcgatcgctt cgaggcggcg
     4861 tggaaccgcg tgcgcgaagg ccgcgccatg cggccgctcg aaattctgag cttcgaggac
     4921 ctgccgctgc tcacccgggc gcttacgccg gcgcgctgga cgctcctgca ggatttgaag
     4981 cgcaagggcc cttgttcgat ctacgagctg gcaaggcgcc tcgggcgcga ctacaagaac
     5041 gtgcacaccg acgtgacgca attggcggcg atcggcctcc tcgagcggcg cgccgacgct
     5101 cttgcctcgg taccgtggga cttgttgcgc gccgagctca aactttagtc cgtccgcagg
     5161 cagcgctaca cgtgcaccgt caaacgggga gagtatcgta gccccatgaa ccgcgatctc
     5221 tcgcccacca gcgcgctcaa cctcgagccg tactggatgc cgttcaccga caaccggcgc
     5281 ttcaaggcga agccaaagct cgtcaccgcg gcgaaggaca tgcacttcac caccgccgag
     5341 ggccggcagg tgctcgacgg gctggcgacg ctctggtgcg tgaacgcggg tcatgcgcgg
     5401 ccgcgcatcg tcgaagcgat ccgccgccag gcgggcgagc tcgacttcgc ggcgtcgttc
     5461 tccctcggcc atccgggcgc gttccgcctc gccgagcgca tcgcggcgct cgcgcccgcg
     5521 ggcatggggc acgtcttctt caccaactcg gggtcggagg cgatcgacac ggcgatgaag
     5581 attgcggtgg cctaccaccg tctgcgcggc gagggcacgc gcacgcgctt cgtcggccgc
     5641 gagcgttcct accatggcgt gaacctcgcg ggaatgtcgc ttggcggcgt gccggcgaac
     5701 cgcaaggcct acagcggcgt gctgctgagc ggggtggacc atctgcgcca cacgcatctc
     5761 gccgagaacg cgttctccag gggacagccg gcacgcggcg ccgagctcgc c
//
LOCUS       DASUNT010000036         8188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_706315, whole genome shotgun sequence.
ACCESSION   DASUNT010000036 DASUNT010000000
VERSION     DASUNT010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8188
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706315"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1040)
                     /locus_tag="VFX94_00725"
     CDS             complement(93..1040)
                     /locus_tag="VFX94_00725"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HEX5766730.1"
                     /translation="MKRILAALAALLLVAAAHAQRIYNQGELDAMLAPIALQPDGVVS
                     QILISATYPEQVAEAARWSRANPHLSGDPAVRAVQNEPWDPAVKALVAFPDLLIRMDE
                     SPQWLRELGEAFVNQEVQVMDTVQALRQRAQAAGHLTSNDNSVVYPQGEAIVVYPRTH
                     YVYVNYYDPYVVYGPWWWPYYRPVYWRPWAPRPVFVAHGFFYTKPDWYRRHVHVVHKP
                     VHVQHHHVTPGKWQHHKHVVSKPYVRVPESQRQPIVQPLHRHMPAASGFSSHKRDFVH
                     RHHQPQARPHAPTHRGHPANAGGGPRMQGNAGGGFRGRG"
     gene            1134..1529
                     /locus_tag="VFX94_00730"
     CDS             1134..1529
                     /locus_tag="VFX94_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766731.1"
                     /translation="MSTAAGVVVGLIVFLAMALASVIAVNGASVRAERAAVQPAAGGA
                     TSTELQPEPEPEGDKSKSLRSLDLDRDGRLSLAEAAGHAEIVQRFHRADRDRDGKLTQ
                     SEYDRLAKLAPAKAGKKKPARRPQTTPQD"
     gene            complement(1432..2247)
                     /locus_tag="VFX94_00735"
     CDS             complement(1432..2247)
                     /locus_tag="VFX94_00735"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5766732.1"
                     /translation="MASLPDSVASWRSLEAEIGPGALRIASYNVHGCRGADGRKDASR
                     IVGVIEECGSDTVGLQEVDYRLDYIAHKLGMEPIPGLTLARHDGPFGNALITRRKVLA
                     VRRLGFTYGGREPRNALDVDLEVAGETVRVIVTHLGLWPAERRYQVRQILKLLKETPI
                     CERVVVLGDINEWLPLGRPLRWMHALFGRSPAERSFPSRWPVVALDRVWVRPRRALLA
                     LKAHRTPLAEQASDHLPVKALVSLAASSAGDARASSSLPSPAQAWRGDRTRIA"
     gene            complement(2219..4465)
                     /locus_tag="VFX94_00740"
     CDS             complement(2219..4465)
                     /locus_tag="VFX94_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX5766733.1"
                     /translation="MGSVLYLDGRLRRRARSAPLRQEALAERAQPHGLQHSVLRLGYN
                     CWALARADRAAFIIDAKDYFHAFYRAALRAQRSIIILGWDFNSQTRLHFDPVPKDGPP
                     ALLGEFLNYLTRRRRRLHVHVLNWDYPMVFGADREFPPMYGFGWTPARRVHLRYDDTH
                     PVAASQHQKVVVIDDELAFVGGIDLTVRRWDCPEHLPQDKRRTAYDAPYPPFHDAMMA
                     VDGEAARRLAELARERWRLATGNALRPIQCDSDPWPAELVPDLTGVDVGIARTLPPRG
                     DLPAVREVEKLYLDMIVAARRTIYIENQYFTAPRIAAALEKRLAEPDGPEVVLVLRLL
                     SHGWLEEATMHVLRTRLIRRLRQVDRFGRFRVYYPHVPGLADGTCVDVHSKLMIVDDD
                     IVRIGSSNLSSRSMALDTECDLVVESRGQARVADAICRFRERLLAEHLDTEPERVREE
                     AEKAGSLNRAIAAFSAQPRTLRQFDDLPEWPEPILSVAAVADPEEPIALERLLSERHI
                     DEVATPQKPAWGNLAGIVLGIVALAAIWRFTPLREIATAEAAVAWAKAFGAQPWAPWA
                     LMASYTPACLVMFPRPLITLAAVIAFGPWLGFAYSLAGICLASAVTWYMGLHMRRDTV
                     RRLAGPRLDRMIEVMKKRGLAAMTLLRLVPLAPFAVESIVAGAVRMKLWHVIAGTAIG
                     LLPGTLTTTVFGDAIETAVTGSGRVNWWLVGGAAALLAAGAWAVKRWFTRMERRMRAH
                     GEPSGQRR"
     gene            complement(4470..5135)
                     /locus_tag="VFX94_00745"
     CDS             complement(4470..5135)
                     /locus_tag="VFX94_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766734.1"
                     /translation="MPSPIRHAFAAALAIGCHGAYAQTAVPPAVTVQRIAPQLVTFAG
                     SQSNFQSLVTGLAQATPVQLVTVLPDGSAQVVNFTPAAAVPAEQIAPTLEAARQRLIG
                     LGIGAPSAEQIAFSLVGGTVPTPLGGAPVNGMLNPQSTPSPAVQVQALSPTTPQSFPL
                     ASQPLAAPRVNTSDSPLPAGATSRTPTLPTTPITAPGAGSTPPTLERATPISPANRAL
                     VRH"
     gene            complement(5201..5788)
                     /locus_tag="VFX94_00750"
     CDS             complement(5201..5788)
                     /locus_tag="VFX94_00750"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5766735.1"
                     /translation="MPPVTQALLFANVAVYLLGGLGVLSLDEFALWPPGGLESRFAPW
                     QLVTYAFLHGGLTHIFFNMLGLYMFGSEVERLFGSRYFLLYYFGCVVTAALTHLAVTG
                     WTGAPPYPTVGASGAIYGLLLAFGVYFPHRKVLLLFPPIPLPARVFVILFAALELVLG
                     VTGTAAGVAHFAHLGGMIGGWLMIQHRRGGFPFRS"
     gene            complement(5790..6629)
                     /locus_tag="VFX94_00755"
     CDS             complement(5790..6629)
                     /locus_tag="VFX94_00755"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX5766736.1"
                     /translation="MALVSQTTTAFDLQGHRGARGHAPENTLAGFERALAIGVDTLEL
                     DVGVTRDGVVVIHHDRRLNPDIARGPDGQWIAAPGPTIHSLTYAELQRYDVGRIRPGS
                     DYAKEFASQEPLDGTRIPRLADLFELVKSTTVRFNIETKLSAEAPDETLAPEPFARAL
                     LAEVRKAGVERRTTIQSFDFRTLALVQREAPDIKTAYLTSRQKGETVPRTVHEAKGAI
                     WSPNFRDLDARALSVAHELGLQVIPWTVNESADIARILKLGVDGIISDYPDRVREALK
                     ARR"
     gene            complement(6647..7024)
                     /locus_tag="VFX94_00760"
     CDS             complement(6647..7024)
                     /locus_tag="VFX94_00760"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5766737.1"
                     /translation="MQLEWNSGLVKHLGIRFVQTEKDRVVAELEVRPELLTTTGALHG
                     GTLMAFADTIGAAGTVVNLEPGQSTATLESKTNFFAAAFDGVVRAEATALHKGKRTQV
                     WQTRITDVKGKLLSLTIQTQVIL"
     gene            complement(7027..7671)
                     /locus_tag="VFX94_00765"
     CDS             complement(7027..7671)
                     /locus_tag="VFX94_00765"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX5766738.1"
                     /translation="MRYELYYWPSIQGRGEFVRLALEEAGTPYINRNPSNDSRAFAPP
                     YLKAGKLLIPQTANILFYLGARLGLAPKSDPERLRLHGMQLTIADWLSEVHDTHHPIA
                     SSLYYEDQKREAKRRAAIFLAERLPKFLRYFERAVDERFSYVHLSLFQMVEGLRYAFP
                     RAMAKAEKKHKMLSRLHDRVAARPRIAAYLASGRRIAFNPYGIFRHYPELDEGG"
     gene            complement(7789..7947)
                     /locus_tag="VFX94_00770"
     CDS             complement(7789..7947)
                     /locus_tag="VFX94_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766739.1"
                     /translation="MKVHLEDGIDVRGHIEHQRGERKRRDELQPAAAHAGGEERPAPG
                     AFGRGAGG"
     gene            7906..8188
                     /locus_tag="VFX94_00775"
                     /pseudo
     CDS             7906..8188
                     /locus_tag="VFX94_00775"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
BASE COUNT         1289 a         2706 c         2899 g         1294 t
ORIGIN      
        1 gaagaagcgc gcgacgcagg tcaagcgaat cgccatccac tgagccgaca gggacctcgc
       61 ccgcgcactc ccgggcggtc cctttgtctt tcctagccgc ggccgcggaa gccgccgccg
      121 gcgttgcctt gcatgcgggg cccgccgccg gcgttcgccg gatgcccgcg atgggtgggc
      181 gcgtggggcc gggcctgcgg ctgatgatgc cggtgcacga agtcacgctt gtgggatgaa
      241 aatccgctcg ccgccggcat gtgccggtgg agcggctgaa cgatcggctg gcgctgcgac
      301 tccggcaccc gaacataggg tttcgaaacc acgtgcttgt ggtgctgcca ctttcccggc
      361 gtgacgtggt gatgttgcac atggaccggc ttgtgcacga cgtgcacgtg gcgccggtac
      421 caatcgggct tggtgtagaa gaagccgtgc gccacgaaaa cgggccgcgg cgcccaaggc
      481 cgccaataga cgggccggta atacggccac caccacggcc cgtacaccac gtatgggtca
      541 tagtagttga cgtagacgta atgcgtgcgc gggtacacca cgatcgcctc gccctgcggg
      601 tacacgacgg aattgtcgtt gctcgtgagg tggcccgcgg cctgcgcacg ctggcgcagc
      661 gcctggaccg tgtccattac ctgtacttcc tggttgacga aggcctcgcc gagctcgcgt
      721 agccactgcg ggctctcgtc catccgaatc agcaggtccg gaaacgcgac cagcgccttc
      781 acggccggat cccacggctc gttttgcaca gcgcgcacgg ccgggtcgcc ggaaaggtgc
      841 gggttcgcgc gcgaccagcg cgcggcctcg gccacctgct ccgggtacgt tgcggagatc
      901 aggatctgcg atacgacgcc gtcgggctgc agcgcgatcg gcgcgagcat cgcgtcgagc
      961 tcaccctggt tgtagatgcg ctgcgcatgc gccgcggcga cgaggagcag ggcggcgagc
     1021 gcggcgagga tccttttcat ggcatcaatt tagcccccgc cgccgagctg gtctgtccca
     1081 atgtgtaacg agttgttgcg ggtccgggca cgtctcctgc ggtgtcgcac gcaatgagca
     1141 ccgccgcggg cgtcgtcgtg ggccttatcg tattcctcgc catggcgctg gccagcgtca
     1201 tcgcggtcaa cggcgcaagc gtgcgcgccg agcgtgcggc ggtgcagccg gccgcgggtg
     1261 gcgctacgag cacggagctt cagcctgagc ccgagccgga aggcgacaag tcgaagtcgc
     1321 tgcgctccct cgacctcgac cgcgacgggc ggctgtcgct cgcggaggcc gcggggcacg
     1381 ctgagattgt ccagcgcttc catcgggccg accgcgatcg cgacggcaag ctcacgcaat
     1441 ccgagtacga tcgcctcgcc aagcttgcgc cggcgaaggc agggaagaag aagcccgcgc
     1501 gtcgcccgca gacgacgccg caagactgac gagggctttt accggcaggt ggtccgaggc
     1561 ctgctcggca agcggggttc gatgcgcctt gagcgcgagg agcgcgcgcc gcgggcgtac
     1621 ccagacgcgg tcgagcgcca cgaccggcca gcgcgaggga aacgagcgct ctgccggaga
     1681 ccggccgaaa agcgcgtgca tccatcgcag cggccgcccg agcggcagcc attcgttgat
     1741 gtcgcccagc acgaccacgc gctcgcagat cggagtttcc ttcaggagct tcaggatctg
     1801 ccttacctgg taacggcgct cggccggcca caggccgaga tgcgtgacga tcacgcgcac
     1861 cgtctcgccg gcgacttcga ggtccacgtc gagcgcgttg cgcggctcgc gtccgccata
     1921 ggtgaatccc aggcgccgca ctgccagcac cttgcgccgc gtgatgagcg cgttgccgaa
     1981 cgggccgtcg tggcgcgcaa gcgtaaggcc cgggatcggt tccatgccga gcttgtgggc
     2041 gatgtaatcg agccgatagt cgacttcctg cagccccaca gtgtcggagc cgcattcctc
     2101 gatcacgcca acgatccggc tcgcgtcctt ccggccgtcg gcgccgcggc agccatggac
     2161 gttgtaggac gcgatccgca gcgcaccggg cccgatctcg gcctcgagtg atcgccagct
     2221 agcgacgctg tccggaaggc tcgccatgcg cgcgcatccg gcgctccatg cgcgtgaacc
     2281 agcgcttcac cgcccaggcg cccgccgcga gcagcgccgc cgcgccgcct accagccacc
     2341 agttcacccg tccggagccc gtgacggcgg tttcgatggc gtcgccgaac accgtggtcg
     2401 tcagcgtgcc ggggagcagc ccgatcgccg tgccggcgat cacgtgccag agcttcatgc
     2461 ggaccgcgcc ggcgacgatg ctctccactg cgaacggcgc gagcggcacc aggcgcagca
     2521 gcgtcatcgc cgcgaggccg cgcttcttca tcacctcgat catccggtcg agcctcggtc
     2581 cggcaagccg gcgcaccgtg tcgcgccgca tatgcaatcc catgtaccag gtgaccgctg
     2641 atgcgaggca gattccggcc aggctgtagg cgaagccgag ccagggtccg aaggcgatta
     2701 cggccgcgag ggtaatgagc ggccgcggga acatcacgag gcaggcaggg gtgtacgagg
     2761 ccatcagcgc ccacggcgcc cagggctgcg cgccgaaggc tttcgcccag gcgaccgcgg
     2821 cttcggccgt cgcgatctcg cgcagcgggg tgaagcgcca gatcgcggcg agcgcaacaa
     2881 tgccgagcac gatgcccgcg aggtttcccc aagcgggctt ctgcggcgtc gcgacttcgt
     2941 cgatgtggcg ctcggagagc agcctttcga gcgcgatcgg ctcctcggga tccgccaccg
     3001 ccgctacgct caggatcggc tcgggccatt cgggcaggtc gtcgaattgc cgcagcgtgc
     3061 gcggctgggc gctgaacgcg gcgatagccc ggttcaggct gcccgctttt tccgcttcct
     3121 cgcgcacgcg ctcgggctcg gtgtcgaggt gctcggcgag cagccgctcg cggaagcggc
     3181 agatcgcgtc ggcgacccgc gcctggccgc gcgactcgac taccaggtcg cattctgtgt
     3241 cgagcgccat cgagcggctg ctcaggttcg acgagccgat gcgcacgata tcgtcgtcca
     3301 cgatcatgag cttggaatgc acgtcgacgc aggtgccgtc ggcaagcccg ggaacgtgcg
     3361 ggtagtacac gcggaagcgg ccgaagcggt ccacctggcg caggcgccgg ataagccgtg
     3421 tgcgcaggac atgcatcgtc gcttcctcga gccagccgtg tgacagcagc cgcagcacga
     3481 gcaccacctc aggtccgtcg ggctcggcga gccgcttttc gagcgccgcg gcaatgcgcg
     3541 gcgcggtgaa gtactggttc tcgatgtaga tcgtgcggcg cgcagcgacg atcatgtcga
     3601 ggtagagttt ctccacctcg cgcaccgcgg gcaggtcgcc gcgcggcgga agagtgcgcg
     3661 cgatgccgac gtcgacgccg gtgaggtcgg gcacgagctc ggcaggccag gggtcggagt
     3721 cgcactggat cggcctgagg gcatttccag tggcgaggcg ccagcgctcg cgcgcgagct
     3781 ccgcgaggcg gcgcgccgct tcgccgtcca cggccatcat ggcgtcgtgg aacggcggat
     3841 aaggtgcgtc gtacgcggtg cggcgcttgt cctgcggcaa atgttccggg caatcccagc
     3901 gccgcaccgt aaggtcgatg ccgccgacga aggcgagctc gtcgtcgatc acgaccactt
     3961 tctggtgctg gctggccgcc accggatggg tgtcgtcgta gcgcaggtgt acccgccgcg
     4021 ccggagtcca gccgaagccg tacatcggcg ggaattcgcg gtcggcgccg aacaccatcg
     4081 ggtagtccca gttgaggacg tgcacatgca agcgccgacg gcgcctcgtc aggtagttga
     4141 ggaactcacc gaggagcgcc ggtgggccgt ctttcggcac cgggtcgaag tgcaggcgcg
     4201 tctggctgtt gaaatcccag ccgaggatga tgatcgagcg ctgcgcgcgc agcgccgccc
     4261 ggtagaaggc gtggaaatag tccttcgcgt cgatgatgaa cgcggcacgg tctgcgcggg
     4321 cgagcgccca gcagttatag ccgagccgca gcacgctgtg ctgcaagccg tgcggctgcg
     4381 cgcgctcggc gagcgcttcc tgccgcaagg gtgcgctgcg cgcgcggcgg cgcagcctgc
     4441 cgtcgaggta cagcacgctg cccatcgcgc tagtggcgca cgagggccct gtttgcggga
     4501 gagatcggcg tcgcgcgctc cagcgtgggc ggcgtcgagc cggcgccggg cgcggtgatc
     4561 ggcgtggtcg gcagcgtggg cgtgcggctc gtcgcgccgg cgggcagcgg gctgtcgctc
     4621 gtgttcacgc gcggcgccgc gagcggctgg ctcgcgagcg ggaagctctg cggggtcgtg
     4681 ggggagaggg cctgcacctg taccgccggg ctcggggtgc tctgcggatt gagcatcccg
     4741 ttgaccggcg ccccgcccag cggggtcggt acggtgccgc ccacgagcga aaatgcgatc
     4801 tgctcggcgc tcggagcgcc gatgcccagg ccgatcaggc gctggcgcgc cgcttccagc
     4861 gtcggcgcga tctgctctgc cggcacggcc gcagccgggg tgaagttcac gacctgcgcc
     4921 gagccgtccg gcaggacggt cacgagctgc accggggtgg cctgcgcgag cccggtcacc
     4981 aggctttgaa aattgctctg cgagccggcg aacgtgacga gctgcggcgc gattctctgc
     5041 acggttacgg ccggcgggac ggcggtctgc gcatacgcgc cgtggcagcc tatggcgagc
     5101 gcggctgcga aggcgtggcg gatcggcgac ggcatgaggc ctcctttcgg cggcctccat
     5161 gcacttttcg tgccttcaat ccccgaaggg aaatccgaat tcagcttctg aaaggaaatc
     5221 cgccgcgccg gtgctggatc atcagccagc cgccgatcat gcctccgagg tgggcgaagt
     5281 gcgccacgcc ggccgcggtg ccggtcacgc cgaggaccag ctcgagcgcg gcgaagagga
     5341 tcacgaacac gcgtgccgga agcgggatag gcgggaaaag aagcagcact ttgcggtgcg
     5401 ggaaatagac gccgaaggcg agcagcaagc cgtaaatcgc tccggaggcg cccaccgtcg
     5461 ggtagggcgg cgcgccagtc cagcccgtca cggcgagatg tgtcagggcg gcggtgacca
     5521 cgcagccgaa gtagtacagg agaaagtagc gcgagccgaa gaggcgttcg acctccgagc
     5581 cgaacatgta caggcccagc atgttgaaaa agatgtgcgt gaggccgccg tgcaggaagg
     5641 cgtaggtcac cagctgccac ggcgcaaagc gcgactcgag cccgcccggc ggccacagcg
     5701 cgaactcatc gagcgacagg acgccgagcc cgcccagcag atagaccgcg acattggcga
     5761 agagcagcgc ctgggtgacc ggcggcaagt cagcgcctcg ctttcagggc ttcgcgcacg
     5821 cggtccggat agtcgctgat aatgccgtcg accccgagct tgaggatgcg cgcgatatcg
     5881 gccgactcgt tgaccgtcca tgggatgacc tgaagcccga gctcgtgcgc aacgctcagc
     5941 gcccgtgcgt cgaggtcgcg gaagttcggc gaccagatcg cgccctttgc ctcgtgcacc
     6001 gttcgcggaa cggtctcgcc cttttgcctg gaggtgagat aggccgtctt gatgtctggc
     6061 gcttcgcgct gcacgagcgc gagggtgcgg aaatcgaacg actggatcgt ggtacgccgc
     6121 tcgacgcccg ccttgcggac ctcggcgagc agcgcgcgcg cgaagggctc cggcgcgagc
     6181 gtctcgtcgg gcgcctcggc cgagagcttg gtctcgatgt tgaagcgcac cgtagtgctc
     6241 ttcacgagct cgaacaggtc cgcgaggcgc gggatgcgcg tgccgtcaag cggctcctgg
     6301 ctcgcaaact ccttcgcgta gtccgatccc ggtcgtatgc ggcccacgtc gtagcgctgg
     6361 agctccgcgt aggtgagcga atggatcgtc ggcccgggcg cggcgatcca ctgcccgtcg
     6421 gggccgcgcg cgatgtccgg gttgaggcgc cgatcgtggt ggatcaccac gacgccgtcg
     6481 cgcgtcacgc cgacgtcgag ctcgagcgtg tcgacgccga tggcgagcgc gcgctcgaag
     6541 ccggcgagcg tgttctccgg cgcgtggccg cgcgcgccgc gatggccctg caggtcgaac
     6601 gcggttgtgg tttgagaaac cagggccatg aacagcagaa gctcgcttat agaatcacct
     6661 gcgtctggat cgtaagcgag aggagcttgc ctttcacgtc cgtgatacgc gtctgccaca
     6721 cctgggtgcg cttgcccttg tgcagcgcag tcgcctccgc gcgcaccacg ccgtcaaagg
     6781 cggcagcaaa gaagttcgtc ttcgattcga gcgtcgcggt actctgcccc ggctcgagat
     6841 tcacgaccgt gccggcggcg ccgatcgtgt cggcgaacgc catcagcgtc ccgccgtgca
     6901 gcgctcctgt cgtggtgagg agctcgggcc gcacctcgag ctctgccacg acgcggtcct
     6961 tctccgtctg cacgaagcgg atgccgaggt gcttcaccag gccgctgttc cactcaagct
     7021 gcattctcat cctccttcgt cgagctcggg atagtggcgg aagataccgt acgggttgaa
     7081 cgcaatgcgc cgcccggacg cgagatacgc cgcgatgcgc ggccgcgctg cgacccggtc
     7141 gtgcaatctc gagagcatct tgtgcttctt ctccgctttt gccatcgctc tcggaaaggc
     7201 gtaacgcaga ccctcgacca tctggaacag cgaaaggtgc acgtacgaga agcgctcgtc
     7261 taccgcgcgc tcgaagtagc gcaggaactt cggcagccgc tcggcgagaa agatcgccgc
     7321 acggcgcttc gcctcgcgct tctggtcctc gtaatacagg ctcgaggcga tggggtgatg
     7381 ggtgtcgtgc acttcgctca gccagtccgc aatggtgagc tgcatgccat gcagtcgcag
     7441 ccgctcgggg tcactcttcg gcgcgaggcc gagccgtgcg ccgaggtaga agaggatgtt
     7501 cgccgtctgc gggatgagga gcttcccggc cttcagatag ggcggcgcga acgccctgga
     7561 atcgtttgaa ggatttctgt tgatgtaggg cgtgcccgcc tcttccagcg ccagccgcac
     7621 gaactcgccg cgtccctgga tcgatggcca gtagtaaagc tcgtaacgca tgctgcgaaa
     7681 ggtacattag gccgtgcggc acgggcatgc cgtttgcaaa gcatgggggt ggcgcgcgca
     7741 acagtgacgt cctgcggcac ctgcggcctc gtccaggtgg tagacgcgct acccgccggc
     7801 gccgcggccg aatgcacccg gtgcgggtcg ttcctcgccg ccggcgtgag ccgcggccgg
     7861 ctggagctcg tcgcggcgct tgcgctcgcc gcgctggtgc tctatgtgcc cgcgaacatc
     7921 tatcccatcc tcaagatgca ccttcacggc tcttactcgg agagcaccgt gggacggcgt
     7981 ggtcacgctc atgacgtaca acgagtgggt cgtcgcgacg atcgtgttcc tcgcgagaat
     8041 ggtgataccg ctcgtgaagg tcgccggcct gcttgcgctc gtcgtgagcg cgtggatggg
     8101 gtggggccgg cgcctgcgcg agcgcacgca gctacgcgcc gctggtgcgc gcgggctcgg
     8161 tgttctggaa cacgagcgcc gtgcaggc
//
LOCUS       DASUNT010000037         2738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_969254, whole genome shotgun sequence.
ACCESSION   DASUNT010000037 DASUNT010000000
VERSION     DASUNT010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969254"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VFX94_00780"
     CDS             complement(<1..906)
                     /locus_tag="VFX94_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5766740.1"
                     /translation="MTLVVLATDALLWLLVAAVIAYALYCRRRAHLAAPWQRVFQSTA
                     AVAPAVVLACFLLAALADSLHFRAAGSVEVHSLLDRALSALRQQSEKTYSAPLATRLY
                     AKEQIETPEGKNVRDYPRLRHGGAHLENETDRTADVAAKAAIGLCAAGVLWALLYVAS
                     RPVARRYPYVPWRAAWTTTALILAFAGPVVSLAPHYHVLGTNQVGQDVLYLALKSIRT
                     SIAIGTLTTLVLLPLGIALGVMAGYFRGWIDDAIQYLYTTLNSIPAVLLIAAAVLMMQ
                     VTIDRHPEWFSTAAQRADFRLLVLCA"
     gene            complement(903..1880)
                     /locus_tag="VFX94_00785"
     CDS             complement(903..1880)
                     /locus_tag="VFX94_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5766741.1"
                     /translation="MLAYIVRRVLYAIPILIGVNLITFVLFFVVNTPDDMARMHLGAK
                     RVTPEAIEKWKQERGYDKDLLWNESAPGAARFTDTIFFEKSLKLFVFDFGRADDQRDI
                     GHEIRTRMGPSLALAIPVFLVGLAVNITFALLMAFFRATYIDFWGVVLCVAMMSVSAL
                     FYIIGGQYLASKILHLVPISGYAGGIDAARFVILPVLIGVVSGIGSGSRWYRTIFLEE
                     MGRDYVRTARAKGLAESVVLYRHVLKNAMIPILTGSVVVIPLLFMGSLLSESFFGIPG
                     LGSYTIDAIQAQDFAVVRSMVFIGSVLYIVGLLLTDLSYTLVDPRIRLE"
     gene            complement(1880..>2738)
                     /locus_tag="VFX94_00790"
     CDS             complement(1880..>2738)
                     /locus_tag="VFX94_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5766742.1"
                     /translation="MEEYLSIFLNGRGVVAHSPLPPGIFGAREGRDAMNPVTHVWREG
                     RESRRPLAEAKKLLAEAGYPDGRDAKTGAPLVLYFDTASRGPGDKARLDWWRKQFEKL
                     SIQLEVRETDWNRFQEKIRKGTQQVYGLGWHADYPDPENFLFLLHGPQARADGAGSNS
                     SNYTSPEFDALFERMKNMPNSAERQQLIDRMVDIARRDAPWIFGFHPKDYSLRHGWLA
                     NAKPNDMAGNTMQYLRLDPALRAGLRREWNRPVVWPVALLLAVLIASAIPAFLAYRRR
                     ERMAARPAA"
BASE COUNT          501 a          862 c          958 g          417 t
ORIGIN      
        1 cgcgcagagg acgagcagcc tgaagtccgc gcgctgcgcc gcagtcgaaa accactcggg
       61 gtgccggtcg atcgtcacct gcatcatgag cacggcagcg gcgatgagaa gcaccgccgg
      121 aatggaattg agggtcgtgt agaggtactg gatcgcgtcg tcgatccagc ccctgaagta
      181 gcccgccatg acgccgagcg cgatgccgag cggcagcagg acgagcgtgg tgagcgtgcc
      241 gatcgcgatg ctcgtgcgga tggacttgag ggcgagatag agaacgtcct gcccgacctg
      301 gttcgtgccg aggacgtgat agtgcggcgc gaggctcacg accgggccgg cgaacgcgag
      361 gatcagcgcg gtggtggtcc aggcagcgcg ccacggtacg tacggatagc ggcgggcgac
      421 gggccgcgag gcgacataga ggagcgccca gagcacgccc gctgcgcaca gaccgatggc
      481 cgctttcgcc gccacgtcgg ctgtgcgatc cgtttcattc tcgagatgcg caccgccatg
      541 gcgcaggcgc gggtagtcgc gcacattctt gccctccggc gtctcgatct gctccttcgc
      601 atagagccgg gtcgcgagcg gcgcggaata ggtcttttcg ctctgctgcc gcagcgctga
      661 cagcgcgcgg tcgagaagcg aatgcacctc taccgagccc gcggcgcgga agtgaagcga
      721 gtcggcgagt gctgccagga gaaagcacgc gagcaccacg gcgggagcca ccgccgccgt
      781 cgactggaat acgcgctgcc agggcgccgc gaggtgcgct cggcgccggc agtagagcgc
      841 gtaggcgatc accgccgcga cgagcagcca gaggagcgca tcggtggcga ggacgaccag
      901 cgtcattcga gcctgatgcg cgggtccacc aacgtgtagg agagatcggt gaggagcagc
      961 ccgacgatgt agagcaccga gccgatgaag accatggagc gcaccacggc gaagtcctgc
     1021 gcctggatcg cgtcgatggt gtagcttccc agccccggga tgccgaagaa cgactcgctg
     1081 agcaggctgc ccatgaagag aagcgggatg acaacgaccg acccggtgag gatcggaatc
     1141 atcgcgtttt tcagcacgtg ccggtaaagc accacggact cggcgaggcc cttcgcgcgc
     1201 gcggtgcgga catagtcgcg ccccatctcc tccaagaaga tggtgcggta ccagcggctg
     1261 cccgatccga taccgctcac cacgccgatc agcaccggca ggatcacgaa cctggcggca
     1321 tcgatcccgc ccgcgtaacc cgagatcggc acgagatgca atatcttcga ggcgaggtac
     1381 tggccgccga tgatgtagaa gagcgcggaa accgacatca tcgccacgca aagcaccacg
     1441 ccccagaaat cgatgtaggt cgcgcggaag aacgccatca ggagcgcgaa ggtgatgttc
     1501 accgcgagcc cgacgaggaa cacagggatg gcaagcgcga ggctggggcc catgcgggtg
     1561 cggatctcgt gcccgatgtc gcgctggtcg tcggcgcggc cgaaatcgaa cacgaaaagc
     1621 ttgagcgatt tttcgaagaa gatcgtgtcg gtgaacctcg ccgcgcccgg agcgctttcg
     1681 ttccacagca ggtccttgtc atagccgcgt tcctgcttcc acttctcgat cgcctcgggc
     1741 gtgacgcgct tggcgccgag gtgcatgcgc gccatgtcgt cgggcgtgtt taccacgaaa
     1801 aacagcacga acgtgatgag gttgaccccg atcaggatcg ggatcgcgta gagcacgcgc
     1861 cggacgatgt aggcgagcat caggccgcgg gccgcgcggc catgcgctca cgccgtcggt
     1921 aggcgagaaa tgccgggatg gcggaagcga tcaatacggc gaggagaaga gccacgggcc
     1981 acaccacggg ccggttccac tcgcggcgca gcccggcgcg cagcgccggg tcgaggcgca
     2041 ggtactgcat cgtgttgccg gccatgtcgt tcggcttcgc gttcgcgagc cagccgtggc
     2101 gcaggctgta gtccttggga tggaagccga agatccacgg cgcgtcgcgc cgcgcgatgt
     2161 cgaccatgcg gtcgatgagc tgctgccgct cggcggagtt cggcatgttc ttcatgcgct
     2221 cgaagagcgc gtcgaactcg gggctcgtgt agttcgagct gttcgagcct gcgccgtcgg
     2281 cgcgcgcctg cgggccgtgc agcaggaaaa ggaagttctc cggatcggga tagtcggcat
     2341 gccagcccag gccgtagacc tgctgcgtgc ccttgcggat tttctcctgg aagcggttcc
     2401 agtcggtctc gcgcacctcg agctggatcg agagcttttc gaactgcttg cgccaccagt
     2461 cgaggcgcgc cttgtcgccc ggcccgcgcg aggccgtatc gaaatagagc acgagcggcg
     2521 cgccggtttt cgcatccctg ccgtcgggat agccggcttc ggcgagaagc ttcttcgcct
     2581 cggcgagcgg ccggcggctc tcgcggccct cccgccacac gtgggtcaca gggttcatgg
     2641 cgtcgcggcc ttcgcgcgcg ccgaagatgc ccggcggcag cgggctgtgc gcgacgacgc
     2701 ctcggccgtt cagaaagatc gacaggtact cctccatg
//
LOCUS       DASUNT010000038         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_706466, whole genome shotgun sequence.
ACCESSION   DASUNT010000038 DASUNT010000000
VERSION     DASUNT010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706466"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VFX94_00795"
     CDS             complement(<1..335)
                     /locus_tag="VFX94_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5766743.1"
                     /translation="MSFTDGYAEVDGCKTRLRRGGTGAPLLFLHGANGAPVVMPFMEK
                     LAQRFDVLVPEHPGYGKSDEPEWLENIHDLAYFYLDFLKALSLSNVTLVGTSMGGWIA
                     MEMAVRDTAR"
     gene            complement(332..1237)
                     /locus_tag="VFX94_00800"
     CDS             complement(332..1237)
                     /locus_tag="VFX94_00800"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX5766744.1"
                     /translation="MSLLQSRLRSGQFVVTAEITPPVSTDPAEFLRRALPLKGLATAI
                     NVTDGAGAKVHLSSLAAAHFLVQSGLEPIFQMTCRDRNRLALQGDLLGAAALGIHNVL
                     VLAGDDPKFGDQPEAKPVYDLDSRALISLARKMSDEGALPSGTAIEKKPELFVGAADV
                     PTDPAPGWTPKSLIGKAQAGADFVQTQFCMDIGIVRRYAARLRESGFALPVLVGIAPI
                     PSARSARWMREKLFGTIVPEAMVERLERAANPKAEGRKICVELLQQLAEVPGIAGAHV
                     MAPMNFAEIPAAIEESGVLKRKRAA"
     gene            1282..2178
                     /locus_tag="VFX94_00805"
     CDS             1282..2178
                     /locus_tag="VFX94_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX5766745.1"
                     /translation="MPELPDVTVYIENLDRLLKGHRLQRVHLFNPFLLRTAVPPISSV
                     EGRRVVQLARMGKRIVVGLEADLHLVVHLMIAGRLRWLGTNQGRKGRNTLALFEFENG
                     TLALTEAGTKRRASLHLVQGEAALRAMDPGGIEVLAADLKAFRERLASANHTLKRALT
                     DPRLFAGIGNAYSDEILHRAKLSPVALTDNLSDEEIQRLYTATRSVLEEWTQKLRTEG
                     FPEKVTAFREGMAVHGRYGKPCPVCGAPVQRIVYAENETNYCARCQTGGRMLADRSLS
                     RLLHSDWPRTLDEWEARRRPAG"
BASE COUNT          364 a          762 c          740 g          367 t
ORIGIN      
        1 cgcgcggtgt cgcgcaccgc catctccatc gcgatccagc cgcccatgga agtgccgacc
       61 agcgtgacat tggaaagcga cagtgctttg aggaaatcga gatagaagta cgccaggtca
      121 tggatgtttt cgagccattc cggctcgtcg gacttgccgt agccggggtg ctcgggcacg
      181 agaacgtcga agcgttgggc gagcttctcc atgaagggca tgacgaccgg cgcgccgttc
      241 gcgccgtgca ggaaaagcag cggcgcgccc gtgccgccgc gccgcaggcg cgtcttgcag
      301 ccgtcgacct ccgcgtagcc gtcggtgaag ctcacgccgc tctttttcgc ttgagcacgc
      361 ccgactcctc gatcgccgcc gggatctcgg cgaagttcat gggcgccatg acgtgcgcgc
      421 ccgcgatgcc ggggacctcg gcaagctgct gcagcagctc cacgcagatc ttgcggcctt
      481 cggctttcgg gttcgccgct ctttcgaggc gctccaccat cgcctccggg acgatcgtgc
      541 cgaagagctt ttcacgcatc cagcgcgccg agcgcgcgga aggaatcggc gcgataccga
      601 ccagcaccgg gagcgcgaag cccgattcgc gcaggcgcgc ggcgtagcgc cgcacgatgc
      661 cgatgtccat gcagaactgc gtctgcacga aatccgcgcc ggcctgcgcc ttgccgataa
      721 ggcttttcgg ggtccagccc ggcgccgggt cggtcggcac atcggccgcg ccgacgaaaa
      781 gctcgggctt cttctcgatc gccgtgcccg aaggcagcgc gccttcgtcg ctcatcttgc
      841 gtgcgagcga aatcagcgcg cggctgtcga ggtcgtacac cggcttcgcc tccggctggt
      901 cgccgaactt ggggtcgtcg ccggccagca cgaggacgtt gtggatgccg agcgcggctg
      961 cgccgagcag gtcgccctgc agcgcaagcc ggttgcggtc gcggcaggtc atctggaaga
     1021 tcggctccag gccgctctgc acgaggaaat gtgccgcggc gaggctggag aggtgaacct
     1081 tcgcgccggc gccgtcggtg acattgatcg cagtggcgag tcccttgagc ggcagcgcgc
     1141 ggcgcaggaa ctcggccggg tcggtggaaa ccggcggcgt gatctcggcg gtgacgacga
     1201 attgcccgct gcgcagcctc gactgcagaa gggacattcg cttgatctta tcatcgggga
     1261 ttcatcccac ctgcccgttt catgcccgag cttcccgacg tcacggtcta catcgagaac
     1321 ctggatcggc tcctgaaggg acaccgcctc cagcgcgtgc atcttttcaa cccttttcta
     1381 ctgcgcactg ccgtgccccc gatctctagc gtggagggca ggcgcgtcgt tcagcttgca
     1441 cggatgggca agcgcatcgt ggtcggcctg gaggccgatc tgcatcttgt cgtgcacctc
     1501 atgatcgccg gccggctgcg ctggctgggc acgaaccaag gcaggaaagg ccgcaacacc
     1561 ctcgcgctct tcgaattcga gaacggcacg ctcgcgctca ccgaggccgg aaccaagcgc
     1621 cgcgcttccc tgcatctcgt ccagggcgag gcggcgctgc gcgccatgga tccgggcggc
     1681 atcgaggtcc tcgccgcgga tctgaaagcc ttccgcgaaa ggctcgcttc ggcgaaccat
     1741 acgttgaagc gcgccctgac cgatccgcgg ctcttcgcgg gaatcggcaa cgcctactcc
     1801 gacgagatcc tgcaccgtgc gaagctctcg ccggtggcgt tgaccgacaa cctgtccgac
     1861 gaagagatcc agaggctcta cactgcgacg cgcagcgtcc tcgaagaatg gacgcaaaag
     1921 ctccgcaccg agggcttccc ggagaaggtc acggctttcc gcgagggcat ggcggtgcac
     1981 gggcgctacg gcaagccctg cccggtgtgc ggcgcgcccg tgcagcgcat cgtctacgcc
     2041 gagaacgaga ccaactactg cgcgcgctgc cagaccggcg gccggatgct ggccgaccgc
     2101 tcgctctccc gcctgctcca ttcggactgg ccgcgcaccc tcgacgagtg ggaggcgcga
     2161 cggcgaccgg caggctgacg gcgccagggc gccggtcgcc gacgccccgt ctgagatttt
     2221 cgacacttga caa
//
LOCUS       DASUNT010000039         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_706522, whole genome shotgun sequence.
ACCESSION   DASUNT010000039 DASUNT010000000
VERSION     DASUNT010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706522"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFX94_00810"
     CDS             complement(<1..443)
                     /locus_tag="VFX94_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5766746.1"
                     /translation="MKLRNKVCLVTGAASGLGKAIAARFAQEGARVAIADLNKAQAQA
                     VADEIKGLGVAMDVTSEEQVNRGVAEVASKWGGVDVLVSNAGIQIVHPVEEFPYADWK
                     KMLAIHLDGAFLTTRACVPHMKRAGRGGSIIYMGSVHSKEASVLKS"
     gene            complement(427..1557)
                     /locus_tag="VFX94_00815"
     CDS             complement(427..1557)
                     /locus_tag="VFX94_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5766747.1"
                     /translation="MERATHEDYILVLQGGGALGAYQAGVFEELSAEGMAPNWVTGIS
                     IGAINCALIAGNAPERRVERLHEFWNIVSSALPKSSFEAVGDYRAFLSSAAALQALVW
                     GVPGFFKPRVPPAPLQPSGTPAATSYYDTTPLLSTLESLVEWDRLNSGEVRLSVSAVN
                     VRTGNHAFFDSAKQRIDVRHVMASGALPPGLPPVEIDGEHYWDGALLSNTPLDYVLEQ
                     PGGGHRTVFQVDLFPARGEFPATIFDASEREKDIRYSSRTRRSTTQEVAWRRVAEAVR
                     RLAAKLPAELRNGPDMQELLARTRITCSGFDIVHLIYRAKQYEHHSKDYEFSRDTMLE
                     HWASGRADVRQTLTDPRWTGREPGVHGVRVFDLLRPEHETAK"
     gene            complement(1560..2000)
                     /locus_tag="VFX94_00820"
     CDS             complement(1560..2000)
                     /locus_tag="VFX94_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX5766748.1"
                     /translation="MRYLEEFAAGQKYGGDARLRVEVERVKSFAAEFDPQPFHLDENA
                     AGASMFKGLAASGWHTAAITMRLLVASDLKPAGGIIGAGFDELRWPLPVRPGDELRVE
                     SEILEVRPLQSRADRGVVKVKTTTLNQRDEAVQVSVGNLFVPRR"
     gene            complement(1997..>2185)
                     /locus_tag="VFX94_00825"
     CDS             complement(1997..>2185)
                     /locus_tag="VFX94_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEX5766749.1"
                     /translation="SAVSIGPRDGGGFGISVKMRVEDRSLSKADLEALVREAHEKICP
                     YSHATRANVDVQFEVVGS"
BASE COUNT          346 a          746 c          713 g          380 t
ORIGIN      
        1 gacttgagca ccgacgcttc tttggagtgc accgagccca tgtagatgat gctgccgccg
       61 cggcccgcgc gcttcatgtg cggcacgcag gcgcgcgtag tgaggaaggc gccgtcgagg
      121 tggatcgcga gcatcttctt ccaatcggcg tagggaaatt cctccaccgg gtgcacgatc
      181 tggatgccgg cgttgctgac cagcacgtcg accccgcccc acttggaagc cacttccgcg
      241 acgccccggt taacctgctc ctcgctcgtc acgtccatcg ccacgccgag gcctttgatc
      301 tcatcggcaa cggcctgcgc ctgtgccttg ttgagatcgg caatcgccac ccgggcgcct
      361 tcttgcgcga agcgcgcagc gatggctttg ccgagcccgc tcgcagcgcc ggtgaccaga
      421 caaaccttat ttcgcagttt catgttcggg cctcagaaga tcgaacacgc gcacgccgtg
      481 cacgccgggc tcgcgtccgg tccagcgcgg gtcggtgagc gtctggcgca cgtccgcgcg
      541 gccgcttgcc cagtgctcga gcatggtgtc gcgtgagaat tcatagtcct tcgaatggtg
      601 ctcgtactgc ttggcgcggt agatgaggtg cacgatgtcg aagccgctgc atgtgatgcg
      661 cgtgcgcgcg aggagctcct gcatgtccgg accgttgcgc agctcggccg gcagcttggc
      721 ggccagcctg cgcacagcct cggcaacgcg ccgccaagcg acctcctggg tcgtgctgcg
      781 ccgcgtgcgg ctcgagtagc ggatgtcctt ctcgcgctcg ctcgcgtcga agatggtcgc
      841 ggggaactcg ccgcgcgcgg ggaagagatc gacctggaac acggtgcggt ggccgccgcc
      901 gggctgctcg agcacgtagt cgagcggcgt gttcgaaagc agcgcgccgt cccagtagtg
      961 ctcgccgtcg atctcgaccg gcggcaggcc gggcggcagc gcgccgctcg ccatgacgtg
     1021 gcgcacgtcg atgcgctgct tcgccgagtc gaagaacgcg tggttgccgg tgcgcacgtt
     1081 gaccgcgctc accgaaagcc gcacctcgcc cgagttcagg cggtcccatt cgaccaggct
     1141 ttccaacgtg cttaagagcg gtgtagtgtc gtaatagctg gttgcagccg gtgttcccga
     1201 aggctggagc ggcgccggcg ggacacgcgg cttgaagaag cccggcacgc cccaaacgag
     1261 cgcttgcaac gccgccgcgc tgctaagaaa cgcgcggtaa tctcctaccg cctcgaacga
     1321 gctttttggc agcgccgagc tcacgatgtt ccagaactca tgtagccgct cgacgcgccg
     1381 ctcgggtgcg ttgccggcga tgagcgcgca attgatcgcg ccaatcgaaa tgccggtgac
     1441 ccagttgggt gccatgcctt cggcggaaag ctcctcgaac acgccggcct gataggcgcc
     1501 gagcgcgccg ccgccctgca gcaccagaat gtagtcctcg tgggtcgcgc gctccacagt
     1561 cagcgtcgcg gcacgaaaag gtttcccacg gagacctgca cggcttcatc gcgctgattg
     1621 agcgtggtcg tcttcacttt gaccacgccg cggtcggcgc gcgattggag cggccgtact
     1681 tcgagaattt cgctttcgac gcgcagctcg tcgcccgggc gcacgggcag cggccagcgc
     1741 agctcgtcga agcccgcgcc gatgatgccg cccgccggct tcagatcgct cgcgacgagt
     1801 aggcgcatgg tgatcgccgc ggtatgccag ccgctcgccg ccaggccctt gaacatggaa
     1861 gcgcccgcgg cgttctcgtc caggtgaaag ggctgcgggt cgaactccgc ggcgaagctc
     1921 ttcacgcgct cgacctcgac tcgcagccga gcatcgccgc cgtacttctg gccggcggca
     1981 aactcttcga gatacctcac gagccgacca cttcgaactg aacgtcgacg ttggcgcgcg
     2041 tggcatgcga gtacgggcag atcttctcgt gcgcctcgcg caccagcgct tcgaggtcgg
     2101 ctttcgacag gctgcgatcc tcgacccgca tcttcacgga aatgccgaag ccaccgccgt
     2161 cacgcggccc gatcgacacc gcgct
//
LOCUS       DASUNT010000040         4396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_268043, whole genome shotgun sequence.
ACCESSION   DASUNT010000040 DASUNT010000000
VERSION     DASUNT010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268043"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..880)
                     /locus_tag="VFX94_00830"
     CDS             complement(65..880)
                     /locus_tag="VFX94_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX5766750.1"
                     /translation="MPARYPERIVCLTEESTETLYLLGEERRIVGISGYTVRPPRARR
                     EKPRVSAFLSAKTDKILELKPDLVIGFSDLQADIARELAKAGLNVLIFNQRSVEEILS
                     MILTLSSLIGQADKGAALVRNLEEGLARMREQARAFPRRPRVYFEEWDEPMISGIRWV
                     SELIEIAGGEDVFASQSHSQAASGRIVAEPTSVVERNPEVILGSWCGKKFRPERVAAR
                     PGWERISAVKDHQLFEIKSADILQPGPAALTDGVKRIHELLAGYLKRSSGRGS"
     gene            897..2030
                     /locus_tag="VFX94_00835"
     CDS             897..2030
                     /locus_tag="VFX94_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX5766751.1"
                     /translation="MGPLQGIRVLEFEAIGPAPFAGMLLADMGADVLVLDRAGETDLG
                     LKRERRHDVLLRGKRSVTVDLKTRSGSEVALRLVCRADALIEGMRPGVMERLGLGPDE
                     VLKRNPRLVYGRMTGWGQDGPLAPRAGHDINYIALAGVLHAFGRKGEAPVPPLNLVGD
                     FGGGGMLLAFGVACGLIEAARSGRGQVIDAAMVEGASLLAAMFAGFLATKSWSEERGT
                     NILDTGAPWYNVYETKDGKYVSIGAIEGRFYEELLRKLGVPDLGQHDRSRWREMHSMF
                     SRSFKSKTREEWCRIFEGSDACFAPVLSWSEARRDPHMAARKNFIEAAGVQQPAPAPR
                     FSRTPGAVRRAPPERGEGGRQALADWGFSAAEIDRLAAQGLGC"
     gene            complement(2037..2474)
                     /locus_tag="VFX94_00840"
     CDS             complement(2037..2474)
                     /locus_tag="VFX94_00840"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX5766752.1"
                     /translation="MRLLAAALFLFTLQALAQGDIEVREAWSRATPPGAKLGVAYMEI
                     RNRGPQAERLIAASTPLARSVEMHVTQRDGEVMKMRQVQGFDIPARERITLRPGGSHL
                     MLVDIAKPLEKGERFRMRLRFERAGEMEIELQVQEQGSRHPGH"
     gene            complement(2471..4096)
                     /locus_tag="VFX94_00845"
     CDS             complement(2471..4096)
                     /locus_tag="VFX94_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEX5766753.1"
                     /translation="MFTRHFAHWPPGQPKTLELPRETVYANLAASAARYPQRAAIDYY
                     GSRISSARLKQEVDALAGFLQRQIRVARGDRVLLYAQNCPQFVIAYYAILRADAVVVP
                     VNPMNRTEELRHYVEDSGASAAIAGNDVLCEIAPLGMERILPIVYSDYLKSATDLPVP
                     DFVRADGPAVWNEMLQADLEPGPHLAGADDLAVMPYTSGTTGKPKGCMHTHATVQATT
                     VPYLYWRGAQEAAVVLCAMPMFHVTGMQAGMNAPIHTAATLVVLSRWDRDCAAMQIER
                     ARVTNWSAITTMLVDFLSNPNLSRFDLSSLRVLGGGGAAMPEALARRLEQVIGLPFVE
                     GYGLSETMAPTHINPPHRPKRQCAGIPIHNTDARILDVQDLHELGPGQVGEIVVHGPQ
                     VFRGYWNQPEATAQAFIEHDGKPFFRTGDLGYYDEEGYFFITDRLKRMINCSGFKVWP
                     AEVESMLYAHPAIQEACVIGTPDGYRGETVKAVVILKKNQKDTSSKDIIDWARERMAA
                     FKVPRVVQFVDELPKTATGKILWRKLQEEENRK"
     gene            complement(4097..>4396)
                     /locus_tag="VFX94_00850"
     CDS             complement(4097..>4396)
                     /locus_tag="VFX94_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX5766754.1"
                     /translation="AGACDYVMPDAERIGGVTGWMRAAALAQGAGVEMSSHLFPEVSC
                     HLLAATPTAHWLEYVDWADAVLAEPLRVKDGHLMVPDAPGIGLKWDEKAVKQYQL"
BASE COUNT          698 a         1458 c         1496 g          744 t
ORIGIN      
        1 aaggtactgg cgcacgagcg cgacaacgtg cgccgcgaat tcagccggcg gctcgcgatc
       61 gaattcagga accgcggcct gaagaccgct tgaggtagcc ggcgagaagc tcgtggatgc
      121 gcttcacgcc gtcggtcaac gccgccggcc cgggctgcag gatgtcggcc gactttattt
      181 cgaacagttg gtggtccttc acggccgaga tgcgttccca gccgggccgc gcggccactc
      241 gctccggcct gaacttcttg ccgcaccagg agccgaggat cacctcgggg ttcctttcga
      301 cgacgctcgt cggctcggcc acgattcgtc ccgaagcggc ctgcgaatgc gactgtgacg
      361 cgaacacatc ctcgccgccg gcgatctcga tcagctcact cacccagcgg atgccgctga
      421 tcatcggctc gtcccattcc tcgaaataga cgcgcggccg gcgcggaaat gcgcgcgcct
      481 gttcgcgcat gcgcgccagg ccctcctcca gattgcgcac cagggcggcg cccttgtccg
      541 cctgcccgat gagagacgaa agcgtgagaa tcatcgacag gatctcttcg accgagcgct
      601 ggttgaagat gaggacgttc aagcccgcct tggcgagctc gcgcgcgatg tccgcctgca
      661 ggtcggaaaa gccgatcacc aggtcgggct tcaattcgag gatcttgtcg gttttcgcgg
      721 acaggaaggc tgaaacgcgc ggcttttcgc gccgcgcgcg cggcgggcgc accgtgtagc
      781 cggagatgcc cacgatgcgc cgctcctcgc cgagaaggta cagcgtttcg gtgctttcct
      841 cggtaaggca tacgatgcgc tcggggtaac gcgccggcat tctgtattat cgaatcatgg
      901 ggcccttgca ggggatccgc gtcctcgagt tcgaggcgat cgggccggcg ccgttcgccg
      961 ggatgctgct cgccgacatg ggcgccgacg tgctcgtcct cgaccgcgcc ggcgagaccg
     1021 accttggctt gaagcgcgag cgccgccacg acgtgctgct tcggggaaaa aggtctgtga
     1081 ctgtggacct gaagacacga tcgggaagtg aagttgcgct gcggctcgtc tgccgcgccg
     1141 atgcattgat cgaaggaatg cgccccgggg tcatggagcg cctcggcctc ggccccgacg
     1201 aggtgctgaa gagaaacccg cggctcgtct acggccgcat gacgggctgg ggccaggacg
     1261 gcccgctcgc gccgcgcgcg gggcacgaca tcaactacat cgcgctcgcc ggcgtgctgc
     1321 acgccttcgg ccgcaagggc gaggcgcccg tgccgccgct caatctcgtc ggcgacttcg
     1381 gcggaggcgg aatgctgctc gccttcggcg tcgcgtgcgg cctcatcgag gccgcacgct
     1441 cggggcgggg ccaggtgatc gacgccgcga tggtggaagg cgcatcgctc ctcgccgcca
     1501 tgttcgcggg cttcctcgcc acgaaaagct ggtcggagga gcgcggcacc aacatcctcg
     1561 acaccggcgc gccctggtac aacgtctacg aaacgaagga cggcaagtac gtctccatcg
     1621 gcgcgatcga gggccgcttc tacgaggagc tcctgcgcaa gctcggcgtg cccgacctcg
     1681 gccagcacga ccgcagccgc tggcgggaaa tgcattccat gttctccagg agtttcaaga
     1741 gcaagacccg cgaagaatgg tgccgcatct tcgaaggctc ggacgcgtgc ttcgcgccgg
     1801 tgttgtcgtg gtccgaggcg cgccgcgatc cgcatatggc tgcgaggaag aatttcattg
     1861 aagcggcagg ggtgcagcag cccgctccgg cgccgcgctt ctcgcgcacg ccgggcgccg
     1921 tgcggcgcgc gccgccggag cgcggcgaag gcggccggca ggcgctcgcc gactggggct
     1981 tcagcgccgc ggagatcgac cggctcgccg cgcagggact cggctgctga tacggctcag
     2041 tgaccggggt ggcgggatcc ctgctcctgc acctgcagct cgatctccat ctcgcccgcg
     2101 cgctcgaatc gcagccgcat cctgaaccgc tcgccttttt cgagcggctt ggcgatatcg
     2161 acgagcatga ggtgcgagcc gccggggcgc agcgtgattc gctcgcgcgc cggaatgtcg
     2221 aagccctgta cctgccgcat tttcatcact tcgccgtcgc gttgcgtgac gtgcatttcg
     2281 acgctccttg ccagcggcgt cgacgccgcg atcaggcgct cggcttgcgg gccgcggttg
     2341 cggatctcca tgtacgcgac gccgagcttc gcccccggcg gcgtggcgcg ggaccacgcc
     2401 tcgcgcacct cgatgtcgcc ttgcgccagc gcctgcagtg tgaacagaaa aagcgctgcg
     2461 gcaagaagcc tcatttgcgg ttctcctcct cctggagctt gcgccagagg atcttgccgg
     2521 tcgcggtctt gggaagctca tcgacgaact gcacgacgcg cggaaccttg aacgccgcca
     2581 tccgctcgcg cgcccagtca atgatgtctt tcgaagaagt gtctttctgg ttcttcttga
     2641 ggatcaccac cgccttgacc gtctcgccgc ggtagccgtc gggcgtgccg atgacgcacg
     2701 cctcctggat cgcggggtgc gcgtagagca tggactccac ctcggcgggc cacaccttga
     2761 agcccgagca gttgatcatg cgcttcaggc ggtcggtgat gaagaagtag ccttcctcat
     2821 cgtagtagcc gaggtcgccg gtgcggaaaa agggcttgcc gtcgtgctcg atgaacgcct
     2881 gagcggtcgc ctcgggctgg ttccagtagc cgcggaatac ctgcgggccg tgaaccacga
     2941 tctcgcccac ctggcccggg ccgagctcgt ggaggtcctg cacatcgagg atgcgagcgt
     3001 cggtgttatg gatcggaatg cccgcgcact ggcgcttcgg ccggtgcggc gggttgatgt
     3061 gcgtcggcgc catggtctcg gagagcccgt agccttcgac gaacggcagc ccgatcacct
     3121 gctccaggcg ccgcgcgagc gcctcgggca tcgccgcgcc gccgccgccg aggacgcgga
     3181 gcgaggaaag atcgaagcga gaaaggttcg ggttggacaa gaaatccacc agcatggtgg
     3241 tgatcgccga ccagttggtg acccgtgcgc gctcgatctg catggcggcg caatcgcggt
     3301 cccagcgcga gagcaccacg agcgtcgcgg ccgtatgaat aggcgcgttc atacccgcct
     3361 gcatgccggt gacgtggaac atgggcatcg cgcaaagtac gaccgcggcc tcctgcgcgc
     3421 cgcgccagta cagatacggc acggtggtcg cctgcacggt ggcgtgggtg tgcatgcagc
     3481 ctttcggctt gccggtggtt cccgaggtgt acggcatgac ggcgagatcg tccgccccgg
     3541 caagatgagg ccccggctcg aggtccgctt gcagcatctc gttccacacc gccgggccgt
     3601 cggcgcggac gaagtccgga accggcagat cggtcgcgct cttcaggtaa tcggaataga
     3661 cgatcggcag aatgcgttcc atcccgagcg gtgcgatctc gcacagcacg tcgttcccgg
     3721 cgatcgcggc gctcgcgccc gagtcctcga cgtagtggcg cagctcttcg gtgcggttca
     3781 tcgggttgac cggcaccacc actgcgtcgg cgcgcaggat ggcgtagtag gcgatgacga
     3841 actgcgggca gttctgcgcg tagagcagca cgcgatcgcc gcgcgccacg cggatctggc
     3901 gctgcagaaa accggcgagt gcgtccactt cctgcttgag ccgcgcggag gaaatccggc
     3961 tgccgtaata atcgattgcc gcgcgctgcg gatagcgcgc agcgcttgcc gcgaggttgg
     4021 cgtaaaccgt ttcgcggggc agctcgaggg ttttgggctg ccccggcggc cagtgcgcga
     4081 aatgccgggt gaacattcag agctggtact gcttgacggc tttctcgtcc cacttaagtc
     4141 cgatgcccgg tgcatcggga accatgaggt gcccgtcctt cacgcgcagc ggctcggcca
     4201 gcaccgcgtc ggcccagtcc acgtattcca gccagtgcgc ggtgggcgtt gccgccagga
     4261 ggtggcagct cacttccggg aacaggtggc tcgacatctc gacgccggcg ccctgcgcga
     4321 gcgcagcggc gcgcatccag ccggtcacgc cgccgatgcg ctcggcatcc ggcatcacgt
     4381 agtcgcaggc gcccgc
//
LOCUS       DASUNT010000041         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_794300, whole genome shotgun sequence.
ACCESSION   DASUNT010000041 DASUNT010000000
VERSION     DASUNT010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794300"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..1170
                     /gene="pbpG"
                     /locus_tag="VFX94_00855"
     CDS             304..1170
                     /gene="pbpG"
                     /locus_tag="VFX94_00855"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HEX5766755.1"
                     /translation="MQRRLHPLEGIVAVCALFFVLALSVGFAQAGERDTIRKIALRSS
                     SALVQDAETGETVYGKNAEAVVPIASITKLMTAMVVLDAGQDLDEKITLSREDAVQMK
                     GSRSRLRSGLSLTRGELLLLALMSSENRAAAALGQNYPGGLEAFVDAMNAKAAALQMD
                     ESRFVEPTGLSPANVSTASDLAKMVRAAHEYPLIREYSTKSRATVRASGRPLQYGNTN
                     NLVRSSYWEIGLSKTGYISEAGRCLVMQVRLAEKDLIVVLLDSWGKHSRIGDANRLRK
                     WLESGAAPGHSS"
     gene            1131..>2017
                     /gene="egtD"
                     /locus_tag="VFX94_00860"
     CDS             1131..>2017
                     /gene="egtD"
                     /locus_tag="VFX94_00860"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX5766756.1"
                     /translation="MARERRGARPLELAVFRQDVVAGLSRPQKSLPPKYFYDAAGSRL
                     FARICRLPEYYVTRAELALTRQHLAAIARFAGRGRQLIEYGSGEGVKSRLLIRALRPA
                     VYVPIDISEDALRAAARALAREFPWLRIAPLAGDFSRPLDIPVRRGTPSVVYFPGSTI
                     GNLTPDEAHAFLSLSRGVAAKMLVGVDLKKDPAVLHAAYNDKAGVTAAFNLNLLARIN
                     RELGADFDLRRFRHYAFYNPAGGRIEMHLVALESQSVRLGRHRFRFAAGETIHTENSY
                     KYSPEDFSALAGKAGWRCA"
BASE COUNT          346 a          696 c          640 g          335 t
ORIGIN      
        1 cccaactcct tgatgcacaa ggtgaatatg caaggatgtg ggccctgcag caggagcagg
       61 cgcaagccaa ggagctgctg gaaaaagccg tgccgctgta ggccaaacgt aggccaaata
      121 gctaggggcg acgccttggc ccgacagtga aagaattcac gtggattcgc gaagttgcgt
      181 cctaaccact gttttacttg cgtatttttt ttccagtagg caagattgcg acaagcccat
      241 gtgccgttca gggcatggcc aacaacaacg gttagtagct ggtcgaggga gaagattcgc
      301 cgtatgcaac gcagactgca tccgctcgag ggcatcgtcg ccgtgtgcgc gctgttcttc
      361 gtgctcgctc taagtgtggg tttcgcgcag gccggagagc gcgacaccat ccgcaagatc
      421 gcacttcgtt cttcctccgc cctggtgcag gacgccgaaa ccggcgagac cgtctacggc
      481 aagaacgccg aagcggtggt gccgatcgcc tcgatcacca agctgatgac cgcgatggtg
      541 gtcctcgatg ccggccagga cctcgacgag aagatcaccc tcagccgtga agacgcggtg
      601 cagatgaagg gctcgcgctc gcgcctgcgc agcgggctgt cactgacgcg cggcgagttg
      661 ctgctgctcg cgctcatgtc ctcggagaac cgcgctgctg cggcgctcgg ccagaactat
      721 cccggcggcc tggaagcctt cgtcgacgcg atgaacgcca aggccgcggc gctgcagatg
      781 gacgagagcc gcttcgtcga gccgaccggc ctttcgccgg ccaacgtgtc caccgccagc
      841 gacctggcga agatggtgcg tgccgcgcac gaatatccgc tgatccgcga gtactccacc
      901 aagagccgcg ccaccgtgcg cgcctcggga cgcccgctgc agtacggcaa taccaacaac
      961 ctggtgcgct cgagctactg ggagatcggc ctttccaaga ccggctatat cagcgaggcg
     1021 gggcgctgcc tggtgatgca ggtgcgtctg gcggagaagg acctgatcgt ggtactgctc
     1081 gattcgtggg gaaagcattc caggatcggc gacgccaacc gcctgcgcaa gtggctcgag
     1141 agcggcgcgg cgcccggcca ctcgagctag cggtttttcg ccaggacgtc gttgccgggc
     1201 tttcgcgccc gcagaagtcg ctccctccca aatacttcta cgatgccgcg ggcagccgcc
     1261 tgttcgcgcg catctgccgg cttcccgagt actacgtaac ccgcgccgag ctcgcgctca
     1321 cccgccagca cctcgcggcc atcgcgcgct tcgccggccg cggccgccag ctgatcgagt
     1381 acggcagcgg cgagggcgtc aagagccgcc tgctgatccg cgcgctgcgc ccggcggtct
     1441 acgtgccgat cgacatctcc gaggacgccc tgcgcgccgc cgcgcgcgca ctcgcgcgcg
     1501 agttcccgtg gctccgcatc gcgccgctgg ccggcgactt ctcgcggccg ctcgacatcc
     1561 cggtgcgccg gggcacaccg agcgtggtct atttccccgg ctccaccatc ggcaacctga
     1621 cgcccgacga ggcccacgcc ttcctctccc tgtcgcgcgg cgtggcggcg aagatgctgg
     1681 tcggcgtcga cctcaagaag gatcccgcgg tgctgcacgc ggcctacaac gacaaggcag
     1741 gcgtcaccgc tgccttcaac ctgaatctcc tggcgcgcat caaccgcgag ctcggcgccg
     1801 acttcgacct gcgccgcttc cgccattacg cgttctacaa ccccgccggc gggcgcatcg
     1861 agatgcacct cgtggcgctc gaaagccaga gcgtgcgcct gggccgccac cgcttccgct
     1921 tcgccgcggg cgagaccatc cacaccgaga actcctacaa gtattcgccg gaggattttt
     1981 ctgcgctggc cggcaaggcg ggctggcgct gcgcgca
//
LOCUS       DASUNT010000042         2234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_356420, whole genome shotgun sequence.
ACCESSION   DASUNT010000042 DASUNT010000000
VERSION     DASUNT010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356420"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..2198
                     /locus_tag="VFX94_00865"
     CDS             204..2198
                     /locus_tag="VFX94_00865"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX5766757.1"
                     /translation="MPHREFFCLRVALDVPLPRTFEYSLPDGLEARVGDRVAVHFGAQ
                     QKIGVVIEERVTPQLAQSRIKPISALRDDAPRLPEDWIALMHFLSTYYQRPFGETVIG
                     ALPPRLRSVKPLPRKVLESKTQGGGARFIATHQLTQAQAAVVQRVARDFNAFLLHGVT
                     GSGKTEVYLHLVAKALARGMQALILVPEISLTPQLESRFREAFPEANLALMHSGLEDV
                     ARTGAWIAAARGEAGIVLGTRLAVLAPLPKLGLIVVDEEHDSSFKQQEGLRYSARDAA
                     VYRAKLAACPVILGTATPSLETWHNWTLGRYEKLELPERASPGARLPAVATLDMRAEL
                     VEQGFCSKSISAIADRLEKREQSLVFINRRGYAPVLACEACGWNAGCDRCTARMVLHA
                     ADRKLRCHHCGAEASIPRKCPTCGNVDLKPIGRGTQRVEETLAAHFPDARVVRMDRDS
                     ARRRAELSRTLERIRRGEGDILVGTQLLAKGHDFPALTLVVVLNADTALVSTDYRAPE
                     RLFCVLAQVAGRAGRRERPGEVLIQTRYPGHPLFAALAQHDYAGFAQSQLAERRAAGF
                     PPFVFEAALRAEAPKLETALAFLKNAAVAVQPPEEVRVFDPVPHLLTRRANLERAQLV
                     IQSASRPALQEFLAALTQVLFTTPCRTVRWHLDVDPIEFD"
BASE COUNT          377 a          750 c          714 g          393 t
ORIGIN      
        1 cttgacttat gcacatcttc gggccttggc cggacgcttc gcgcgagttt gcgcgttccg
       61 gggctatgaa cttctaaggt actgaatcct tgtcctaaaa cggggcgctt ggcccacagc
      121 gccggggaag ccggcgggta cttggagttt gtgacacgag gatgaagcgt ttactcacag
      181 gtttatccac aaaccctaag tccttgccgc acagagagtt tttttgtctc cgggtcgccc
      241 tcgacgtacc gctgccgaga acgttcgaat attcgttgcc ggacggactg gaggcgcgcg
      301 taggcgaccg cgtggcagtg cacttcggag cgcagcaaaa gattggcgtg gtgatcgaag
      361 agcgcgtgac gccgcagctt gcgcaatcgc gtatcaagcc catcagcgct ttgcgtgacg
      421 acgcgccgcg actacccgaa gactggatcg cgttgatgca cttcctgtcg acgtactacc
      481 aacgtccgtt cggcgagacg gtgatcggtg cactgccgcc gcgcttgcgg tccgtgaaac
      541 cactgccgcg caaagtgctc gagtcgaaga cgcaaggcgg tggcgcgcgc ttcattgcca
      601 cgcaccagct cacgcaagcc caggccgctg tcgtgcagcg cgtcgcccga gacttcaacg
      661 ccttcctgct gcatggagtg accggcagcg gaaagaccga ggtctacttg cacctcgtag
      721 cgaaagcgct tgcgcggggg atgcaagcgc tcatcctcgt tccggagatc agcctgacgc
      781 cgcaactcga gagccggttt cgtgaagctt tccccgaggc caatctcgcc ctcatgcata
      841 gcggcctcga agacgtggcg cgcacgggcg cgtggatcgc ggctgcccgc ggcgaggcag
      901 gcatcgtgct cggcacgcgg ctcgccgtgc tggcgcccct cccgaagctc gggcttatcg
      961 tagtggacga ggagcatgac tcgtcattca agcagcagga agggttgcgc tactcggcgc
     1021 gcgacgccgc ggtttatcgc gcaaaactcg ccgcctgccc ggtaatcctc ggcaccgcca
     1081 cgccgtcact cgagacgtgg cacaactgga cccttgggcg ctacgaaaag ctcgagctgc
     1141 ccgagcgcgc ctcgccgggg gcgcggcttc cggctgtggc gacgctcgac atgcgcgcag
     1201 aactcgtgga gcaggggttc tgcagcaagt cgatctccgc aattgcggat cggctcgaga
     1261 agcgcgagca aagcctcgtc ttcatcaatc gccgcggcta tgcgcctgtg ctcgcgtgcg
     1321 aagcgtgcgg ctggaacgcg gggtgcgacc gatgcacggc gcgcatggtg ctgcatgccg
     1381 ccgaccgaaa gctgcgctgt caccattgcg gcgccgaagc gtccatcccg cgaaagtgcc
     1441 caacgtgcgg caacgtggac ctgaagccca tcggacgtgg gacgcagcgc gtggaggaaa
     1501 ccctcgctgc gcactttccg gacgcccgag tcgtacgcat ggatcgcgac agcgcgcggc
     1561 ggcgcgccga gctttcgcgc acgctcgagc gcatccggcg cggcgagggc gacatcctcg
     1621 tcggcacgca gctcctcgcc aagggacatg attttccggc gctcacgctc gtcgtcgtgc
     1681 tgaacgccga caccgccctt gtctccaccg actaccgcgc gcccgagcgc ctgttctgcg
     1741 tcctggcaca agtggcgggg cgcgctggcc gtcgagagcg gcctggagaa gtgctgatcc
     1801 agacgcgcta cccggggcac ccgctttttg ctgcgctggc gcagcacgac tacgctgggt
     1861 tcgcgcagtc ccagctcgcc gaacggcgcg ccgccggctt tccgccgttc gtgttcgagg
     1921 ccgcgctgcg cgccgaagcg ccgaagctcg agacggcgct cgcattcctg aagaacgccg
     1981 ctgtcgcagt gcagccgccc gaagaggtgc gcgtattcga ccccgttccg cacctgctca
     2041 cccgccgcgc gaacctggag cgtgcacaac tcgtcatcca gtcggcctca cgcccggcgc
     2101 ttcaggaatt tctcgcggct ctcacccaag tccttttcac gactccctgc cgcaccgtgc
     2161 gctggcatct cgacgtggac ccgatcgaat tcgactgacg gctcgggaca acgggttcga
     2221 ttagaatctt cgtt
//
LOCUS       DASUNT010000043         2568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_268457, whole genome shotgun sequence.
ACCESSION   DASUNT010000043 DASUNT010000000
VERSION     DASUNT010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268457"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..938)
                     /gene="serB"
                     /locus_tag="VFX94_00870"
     CDS             complement(108..938)
                     /gene="serB"
                     /locus_tag="VFX94_00870"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEX5766758.1"
                     /translation="MNLVVQGPALEEHQAQELARMAAAKQLQPIAPRAYRLLDPQNRN
                     GIAHWCDTRRIDHAWMPEARSFADLKLIAMDMDSTLITIECIDELGELAGKKAEIAAL
                     TARAMRGEIEYRESLRRRVAALAGLAVDSLQTIYEARLKLTPGADTLLAACKRHGVKL
                     LLVSGGFSFFTERLRERLALDYTISNVLEVRDGRLTGALVGEIVDADAKARKFSEVAR
                     ALRAAPEHTVAIGDGANDLEMMKQAGISVAFRAKPVVRAQATCALSWSGLDGVVNLFE
                     "
     gene            complement(935..>2568)
                     /locus_tag="VFX94_00875"
     CDS             complement(935..>2568)
                     /locus_tag="VFX94_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5766759.1"
                     /translation="TPDQAAAINAVLADMAAGKPMDRLVCGDVGFGKTEVALRAAFAA
                     VADGSQVVMLCPTTLLAEQHFQSFSDRFADWPVRIAELSRFRTGKESSETLEKLASGE
                     IDIVIGTHKLLARDVKLKRLGLVIIDEEHRFGVRQKEALKKLRAEVDVLTLTATPIPR
                     TLAMSLEGIRDFSVIATAPQRRLAIKTFVSPWSEGLIREAVMRELRRGGQAYFLHNEV
                     ESIERMRERLTKLLPEARIAVAHGQMRERELERVMREFTAQRSNVLVCSTIIETGIDI
                     PTANTMVINRADRFGLAQLHQLRGRVGRSHHQAYAYLLTPPAEALNKNAVKRLEAIQM
                     MEELGSGFYLAMQDLEIRGAGEILGEAQSGEIQEVGFTLYARMLERAVRRLKSGKAAD
                     PGDAIDVSTEVNLHVPALLPASYCSDVQERLSLYKRLADAQTHETLELLREELVDRFG
                     ELPEPARALLECHRVRIAARALGVARVDATHEAVQLQFIQDPPLEAAKVIAFIRRKGR
                     AARLAGPDKLRVEAKLPAWQERAQAVKDVLHQLAA"
BASE COUNT          384 a          887 c          859 g          438 t
ORIGIN      
        1 tggacgtcgc agacttgcgc gacaccgcca tgatagctgc tgcggcgcga aagatgcgcg
       61 ctgcccgaga aatactcagg aaataggaca gcgggtcgcc gcgcgtacta ctcgaagaga
      121 ttcaccacgc cgtcgaggcc gctccagctg agcgcgcacg tcgcttgggc gcgcaccacc
      181 ggcttcgcgc ggaacgcaac ggagatgccg gcctgcttca tcatctccag atcgttcgcg
      241 ccatcgccaa tcgccacggt gtgctcgggt gcggcgcgca gcgcgcgcgc cacctcgctg
      301 aacttgcgcg cttttgcatc cgcatcgacg atttcgccga ccagcgcccc ggtcagcctg
      361 ccgtcgcgca cttcaagcac gttggagatg gtgtagtcga gcgcgagccg ctcgcgcaag
      421 cgctcggtga agaagctgaa tccccccgaa accaggagaa gcttcacgcc atgcctcttg
      481 caagcggcga gcagcgtgtc ggcccccggc gtgagcttga gccgcgcttc gtaaattgtc
      541 tgcagcgaat ctaccgccag cccggcgagc gccgccacgc gccggcgcag gctctcgcgg
      601 tactcgattt cgccgcgcat cgcgcgcgcg gtgagcgccg cgatctcggc tttcttcccg
      661 gccagctcgc ccagctcgtc gatgcactcg atcgtgatca gcgtggaatc catgtccatg
      721 gcgataagct tcaggtcggc gaagctccgc gcttcgggca tccaggcatg gtcgatgcgt
      781 cgggtatcac accagtgcgc aatcccattg cggttttgtg ggtcgagcag ccgatacgcc
      841 cgtggggcga tcggttgcag ctgcttcgca gccgccatgc gggcgagctc ctgcgcctga
      901 tgctcctcga gcgcgggacc ctggaccacg aggttcatgc cgcgagctga tgcaggacgt
      961 ctttcaccgc ctgcgcgcgc tcctgccagg ccggcagctt cgcctccacg cgcagcttgt
     1021 cgggcccggc gagccgcgcc gcgcggccct tgcgccggat gaacgcgatg actttcgccg
     1081 cttcgagcgg cgggtcctgg atgaactgca gctgcaccgc ctcgtgcgtc gcgtccacgc
     1141 gcgccacgcc gagcgcccgc gcggcgatgc gcacgcggtg gcattcgaga agcgcgcgcg
     1201 ccggctcggg cagctcgccg aagcggtcga cgagctcttc gcgcagcagc tcgagcgtct
     1261 cgtgcgtctg cgcgtcggcg aggcgcttgt agagcgaaag ccgctcctgc acgtcgctgc
     1321 agtagctcgc aggcaggagc gccgggacat gcagattgac ctcggtggag acatcgatcg
     1381 catcgccggg gtcggcggct ttgcccgatt tcaacctccg caccgcgcgc tcgagcatcc
     1441 gcgcgtaaag ggtgaagccg acctcctgga tttctcccga ctgcgcctcg ccgaggatct
     1501 cgcccgcgcc gcggatctcg aggtcctgca tggcgaggta gaagcccgag ccgagctctt
     1561 ccatcatctg gatcgcctcg aggcgcttga cggcgttctt gttgagcgct tccgcgggcg
     1621 gcgtgaggag atacgcatag gcctggtgat gcgagcggcc gacgcgcccg cgcagctggt
     1681 ggagctgcgc gagcccgaag cggtcggcgc ggttgatcac catggtgttg gcggtcggga
     1741 tgtcgattcc ggtctcgatg atggtggagc agacgagcac attggaccgc tgcgcggtga
     1801 actcgcgcat cacgcgctcc aactcgcgct cgcgcatctg gccgtgcgcc accgcgatgc
     1861 gcgcctcggg caggagcttc gtcagccgct cgcgcatgcg ctcgatcgac tcgacctcgt
     1921 tgtgcaggaa gtacgcctgg ccgccgcggc gcagctcgcg catcaccgct tcgcggatca
     1981 gcccctcgga ccacggcgac acgaaggtct tgattgccag gcgccgctgc ggcgcggtgg
     2041 cgatgaccga gaagtcgcgg atgccctcga gcgacatcgc gagcgtgcgc gggatcggcg
     2101 tcgccgtgag cgtcagcacg tcgacttcgg cgcgcagctt cttcagcgcc tccttctgcc
     2161 gcacgccgaa gcggtgctcc tcgtcgatga tcacgaggcc gaggcgtttg agcttcacgt
     2221 cgcgcgcgag cagcttgtgc gtgccgatga cgatgtcgat ctccccgctc gccaattttt
     2281 ccagcgtttc gctgctctcc ttgcccgtcc tgaagcgcga gagctcggcg atgcgcaccg
     2341 gccagtcggc gaagcggtcg gaaaagctct ggaagtgctg ctcggcgagg agcgtggtgg
     2401 ggcagagcat caccacttgg ctgccatcgg ccaccgccgc aaacgcggcg cgcagcgcga
     2461 cctcggtctt gccgaatccc acatcgccgc acaccaggcg gtccatcggc ttgccggccg
     2521 ccatgtcggc gagcacggcg ttgatcgccg cggcctggtc gggcgtct
//
LOCUS       DASUNT010000044         3250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_970062, whole genome shotgun sequence.
ACCESSION   DASUNT010000044 DASUNT010000000
VERSION     DASUNT010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970062"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFX94_00880"
     CDS             <1..251
                     /locus_tag="VFX94_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX5766760.1"
                     /translation="IPQDILPGAFNSGFKLALMTKDVGIAAELARGLKLKAPFLKETL
                     KQWRAAQKALPRDADHTEIYKYQRKRIRGRSPNSVRRR"
     gene            complement(238..1026)
                     /locus_tag="VFX94_00885"
     CDS             complement(238..1026)
                     /locus_tag="VFX94_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX5766761.1"
                     /translation="MASEKMLSKKEHGVGWMILNNPERHNAISLEMWDAALEIMQDFR
                     ADPSVRVMVLTGAGGKAFASGADISKFKDERQGAAAVAHYQATTQKAYDAIIGMPIPT
                     IAMIRGYCIGGGTAVAVCCDIRVCTENAKFGVPAAKLGLGYGMNRAKPLVELIGPAYA
                     KEMFFTGRQFTAQEAEKMGLVNRVVPDTELEKVVDEMARTIAENAPLTVKCAKLVVGE
                     VLKDADKRDIGATERAVEACFKSADYKEGQAAFMEKRKPRFIGA"
     gene            complement(1026..2195)
                     /locus_tag="VFX94_00890"
     CDS             complement(1026..2195)
                     /locus_tag="VFX94_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX5766762.1"
                     /translation="MQASTALSHIKVIDLTRVRAGPTAVRQLADWGARVIKVEMPAGL
                     DEGEGPGGPREGSDFQNVHRNKRGITLNLKSPDGVAILKKLVAQSDVLVENYRPDVKD
                     RLGIGYAAMAEVNPRLVYGSISGYGEDGPYRMRPGFDQIAQGMGGLMSITGLPGQGPV
                     RVGIPIADLCAGIFCAYGIAVALIERERSGKGQWVKSSLLQAQLFMLDFQAARWLMKG
                     EVAKQAGNNHPTSIPTGVYKTRDGYMNLAVTGTKIWNRMCQAIGAPELANHADYATGA
                     LRSKNRDALHDVLEERFTKRDTAYWIDTLNQAGVPAGPIYSIDQAFADPQVKSLGMVQ
                     KVGEVAYLGQPVTLSRTPSTVVSHPPRQGEHTAEVLRELGYEDEKIDELRKKGIV"
     gene            2237..3208
                     /locus_tag="VFX94_00895"
     CDS             2237..3208
                     /locus_tag="VFX94_00895"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5766763.1"
                     /translation="MANTNRRRVLGFAGGAAALSVLRPLAAQDKFPSRPIEVVTHAGV
                     GGGTDITARMMMVHAPGVFKNEFVVVNRVGGSGAAALQYMLEKPRDGHTIALITQTHL
                     LTILRSKGKFKYEDLVPLARATEDPQILAVGKGSPYKSAQDLFGAGKGKALKYGTSLV
                     GGVDHIAVVSIARSAGMQPPTIVPFRGGGDVVINLVGGNIDCALVNYAEAESQFKAGD
                     VRALAIFADKPLAALPNIPTGKQVGVPGNYSTVRGFVALSGTPAERIGALEEGLVKAM
                     QGQLFQTYLETSGQSMESVVGAGPWKAQLDEFMVEGKKTLEALGLLK"
BASE COUNT          524 a         1071 c         1082 g          573 t
ORIGIN      
        1 agattcccca ggacatcctg cccggcgcct tcaactccgg cttcaagctc gcgctgatga
       61 ccaaggacgt cggcatcgcg gccgagctgg cacgcggcct gaagctcaag gcacccttcc
      121 tgaaagagac gctaaagcaa tggcgcgcgg cgcagaaggc gctgccgcgc gatgcggacc
      181 acaccgagat ctacaaatac cagcgcaaac gaattcgggg acggagccct aattcggtca
      241 ggcgccgatg aatctgggct tgcgcttctc catgaaggcg gcctggcctt ccttgtagtc
      301 ggcgcttttg aagcaggctt ccaccgcgcg ctcggtggcg ccgatgtcgc gtttgtcggc
      361 gtccttgagg acttccccga cgacgagctt cgcgcatttc acggtgagcg gcgcgttttc
      421 ggcgatggtg cgcgccatct cgtcaacgac tttctcgagc tccgtgtcgg gtacgacgcg
      481 attcaccaag cccatcttct cggcttcctg cgcggtgaac tggcggccgg tgaagaacat
      541 ttccttcgcg tacgccgggc cgatcagctc gacgagcggc tttgcgcggt tcataccgta
      601 gccgaggccg agcttggcgg cggggacgcc gaacttggcg ttctcggtgc agacgcggat
      661 gtcgcagcac accgcgaccg cggtaccgcc gccgatgcag tagccgcgga tcatggcgat
      721 ggtggggatc ggcatgccga tgatcgcgtc gtaggccttc tgcgtggtgg cctggtaatg
      781 cgcgaccgcg gcggccccct ggcgctcatc cttgaacttg gagatgtcgg cgcccgaggc
      841 gaacgccttg ccgccggcgc cggtcagcac catgacgcgc accgaagggt cggcgcggaa
      901 gtcctgcatg atctcgagcg cggcatccca catctcgagc gagatcgcgt tgtggcgctc
      961 ggggttgttg aggatcatcc agccgacccc gtgctccttt ttcgaaagca tcttctcgct
     1021 cgccatcaga cgattccttt cttgcgcagt tcgtcgatct tctcatcctc gtagccgagc
     1081 tcgcgcagca cctccgcggt gtgctcgccc tgcctcggcg gatgcgacac cacggtgctc
     1141 ggcgtgcgcg agagcgtcac gggctggccg aggtaggcga cctcgccgac cttctgcacc
     1201 atgccgaggc tctttacctg cgggtcggcg aaggcctggt cgatcgagta gatcgggccg
     1261 gccggcacgc cggcctggtt cagcgtgtcg atccagtagg cggtgtcgcg cttggtgaag
     1321 cgctcctcga gcacgtcgtg cagcgcgtcg cggttcttcg agcgcagcgc gccggtcgcg
     1381 tagtcggcgt ggttcgcaag ctcgggcgcg ccgatcgcct ggcacatgcg gttccagatc
     1441 ttggtgccgg tgaccgcgag gttcatgtag ccgtcgcggg tcttgtagac gccggtcggg
     1501 atcgaggtcg gatggttgtt gcccgcctgc tttgccacct cgcctttcat cagccagcgc
     1561 gccgcctgga agtccagcat gaaaagctgc gcctgcagga gcgaggactt cacccactgt
     1621 cccttgcccg acctttcgcg ctcgatcagc gcgaccgcga tgccataggc acaaaaaatg
     1681 ccggcgcaca gatccgcgat cgggatgccg acgcgcaccg gtccctgccc cggcaagccc
     1741 gtgatcgaca tgagtccgcc catgccctgc gcaatctgat cgaaccccgg gcgcatgcgg
     1801 tagggaccat cttcgccgta gcccgagatg cttccgtaga cgagacgcgg attcacctcc
     1861 gccatcgcgg catagccgat gccgagccgg tccttcacgt cagggcgata gttctcgacg
     1921 agcacgtctg attgcgcgac cagcttcttc aggatcgcga cgccgtcggg cgacttcagg
     1981 ttgagcgtga tgccgcgctt gttgcggtgc acgttctgga aatccgaccc ctcgcgcggc
     2041 ccgcccggcc cctcgccttc gtcgaggccc gcgggcattt cgaccttgat cacccgtgcg
     2101 ccccagtcgg cgagctggcg caccgcggtg ggaccggcgc gcacgcgcgt gaggtcgatc
     2161 accttgatgt gcgagagcgc ggtggaggcc tgcatctttt tgagtttaca ctgcacctcg
     2221 aggaggaaag tccggcatgg cgaatacgaa tcgcaggcgg gtgctcggtt tcgcgggagg
     2281 cgcggccgct ctctcggtat tgaggccgct cgcggcgcag gataagtttc cctcgcgccc
     2341 gatcgaggtg gtgacgcacg ccggcgtcgg cggcggcacc gacatcacgg cgcgcatgat
     2401 gatggtccac gcgccgggcg tgttcaagaa cgagttcgtg gtcgtgaacc gggtcggcgg
     2461 cagcggcgcc gctgcgctgc agtacatgct ggaaaagccg cgcgacgggc acacgatcgc
     2521 gctcatcacg cagacccacc tgctgacgat cctgcgcagc aagggcaagt tcaagtacga
     2581 ggacctcgtg ccgctcgcgc gcgccaccga ggatccgcag atcctcgccg tgggcaaggg
     2641 cagcccgtac aagagcgcgc aggatctctt cggggcggga aaaggcaagg cgctcaagta
     2701 cggcacctcg ctcgtcggtg gcgtggacca tatcgcggtc gtcagcatcg cgcgcagcgc
     2761 cggcatgcag ccgccgacga tcgtgccgtt ccgcggcggc ggcgacgtgg tgatcaacct
     2821 cgtcggcggc aacatcgact gcgcgctcgt caactacgcc gaggccgagt cccagttcaa
     2881 ggcgggcgac gtgcgcgcgc tcgcgatctt cgccgacaag ccgctcgccg cgctccctaa
     2941 catcccgacg ggcaagcagg tcggcgtgcc ggggaactat tcgacggtgc gcggcttcgt
     3001 cgccctgtcc ggaacacccg ccgagcggat cggggctctc gaggaagggc tggtgaaggc
     3061 catgcaggga cagcttttcc agacctacct cgaaacctcc gggcagtcga tggagagcgt
     3121 cgtcggcgcg ggcccctgga aggcgcagct cgacgagttc atggtcgaag gcaagaagac
     3181 gctcgaagcg ctcggactgc tgaaataggg tgacggcaaa ccgcattgcc gcggccgcgg
     3241 tcatcgccgt
//
LOCUS       DASUNT010000045         3220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_269310, whole genome shotgun sequence.
ACCESSION   DASUNT010000045 DASUNT010000000
VERSION     DASUNT010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269310"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VFX94_00900"
     CDS             <1..525
                     /locus_tag="VFX94_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX5766764.1"
                     /translation="VQMFLWFFVMPELLPTELGDWIKQMPPPWSSYIPAVLCLGIFTS
                     VRIAEQVRAGINSLPRGQHMAGTALGLTLGQAYRYVILPQAFRIILPPLTSESMNIIK
                     NSSVALTIGLLELTGRARAMQEFSFRVFEAFAAATVIYLLTNLVVVLAMRALERKVRV
                     PGLIAAGGAPQAGH"
     gene            526..1218
                     /locus_tag="VFX94_00905"
     CDS             526..1218
                     /locus_tag="VFX94_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331911.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5766765.1"
                     /translation="MDSGFDFDVIQRSWGYLFREGMTFTVTLTLLAMAGGIIFGTLLA
                     MMRLSAFKPLSMLAGGYVNLMRSVPLVLVIFWFFFLVPYIGAWVTGASQPIKVGTWLS
                     ALVTFTMFEAAYYCEIMRAGIQSIARGQVWSGYALGLNYWQTMGYIVLPQAFRNMVPV
                     LLTQTIILFQDTSLVYVISATDFLGAASKVANRDYRLVEMYTFVAVVYFIISYSLSLL
                     VKRLQDRIAIIR"
     gene            1215..1943
                     /locus_tag="VFX94_00910"
     CDS             1215..1943
                     /locus_tag="VFX94_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX5766766.1"
                     /translation="MISIRNVSKWYGSFQVLADCSTEVAKGEVVVVCGPSGSGKSTII
                     KCVNALEPFQKGEITVEGISVGDPKTNLPKLRSRIGMVFQNFELFPHMSVTENLTLGQ
                     VKVLGRSQDEARAKGLKLLERVGLMAHKDKFPGQLSGGQQQRVAIARALSMDPIAMLF
                     DEPTSALDPEMINEVLDVMVALAREGMTMMVVTHEMGFARKVAHRVIFMDGGRIVEDA
                     KKDEFFGTPRSERAQQFLSKILHH"
     gene            2140..2547
                     /locus_tag="VFX94_00915"
     CDS             2140..2547
                     /locus_tag="VFX94_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766767.1"
                     /translation="MLARLNCDCLPAEMERVASDLSAGKANATATPAAFLSRVKTAFN
                     ACAARIARADIVASCAAGDASKLGVANKPIYCGCLSEGLKALDDDTIVSAAARVHRNS
                     EDRVRARSKGRPEPVIAPSAVDSLEQRCKELAG"
     gene            complement(2557..3183)
                     /locus_tag="VFX94_00920"
     CDS             complement(2557..3183)
                     /locus_tag="VFX94_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766768.1"
                     /translation="MRTDDDKVSRRYRELSREEPPPQLDDAILAAARRGVEARPAPLV
                     APAGRRRWYVPLAAAAIIVLAVAVTVHVERQRPDMEVAEAPARHEPAPAAQDPKPEPP
                     RESRAQRKAEALDDLEKRQVAPAAPARADAAASPQLRAEAQGVMREKAAALEARAPAS
                     PEQWLQGIADLRRQGRHEEADKALAEFRRAYPDYRISEAMLEKVEKQP"
BASE COUNT          513 a         1084 c         1046 g          577 t
ORIGIN      
        1 gtgcagatgt tcctgtggtt cttcgtgatg cctgagctcc tgccgaccga gctcggcgac
       61 tggatcaagc aaatgccgcc gccgtggagc tcgtacatcc cggcggtgct ctgccttggc
      121 atcttcacgt ccgtgcgcat cgccgagcag gtgcgcgccg gcatcaactc gctgccgcgc
      181 ggccagcaca tggcgggcac cgcgctcggc ctcacgctcg gccaagccta ccgctacgtg
      241 atcctgccgc aggccttccg catcatcctg ccgccgctca cttccgagtc gatgaacatc
      301 atcaagaact cctcggtcgc gctcaccatc gggcttctcg agctcaccgg gcgcgcgcgc
      361 gcgatgcagg agttcagctt ccgcgtgttc gaggcgttcg ccgcggcgac cgtgatctac
      421 ctcctcacca acctggtggt ggtgctcgcg atgcgcgcgc tcgaacggaa ggtgcgcgtc
      481 cccggcctga tcgccgccgg cggcgcgccg caggcgggac actagatgga ttcggggttc
      541 gatttcgacg tcatccagcg ctcgtggggc tacctcttca gggagggcat gacgttcacg
      601 gtcacgctca cgctgctcgc gatggcgggc ggcatcatct tcggcacgct gcttgccatg
      661 atgcggcttt cggcgttcaa gccgctctcg atgctcgccg gcggctacgt caacctgatg
      721 cgctctgtgc cgctcgtgct ggtgatcttc tggttcttct tcctggtgcc ctacatcggc
      781 gcctgggtca ccggggcgag ccagccgatc aaggtcggca cctggctgtc ggcgctcgtc
      841 accttcacca tgttcgaggc cgcgtactac tgcgagatca tgcgcgccgg catccagtcg
      901 atcgcgcgcg gccaggtctg gtcgggctac gcgctcggcc tcaattactg gcagaccatg
      961 ggctacatcg tcctgccgca ggcgttccgc aacatggtgc cggtgctgct cacccagacc
     1021 atcatcctgt tccaggacac ttcgctcgtt tacgtgatct cggccaccga ctttctcggc
     1081 gccgcctcca aggtcgccaa ccgggactac cgcctggtcg agatgtacac cttcgtcgcg
     1141 gtggtgtatt tcatcatctc ctattcgctg tcccttctcg tcaaacggct tcaggacagg
     1201 atcgccatca tccgatgatc tcgatcagga acgtcagcaa gtggtacggc agcttccagg
     1261 tgctcgccga ctgctcgacc gaagtcgcca agggcgaggt ggtggtggtg tgcggcccct
     1321 cgggcagcgg caagtccacc atcatcaagt gcgtcaacgc gctcgagccc ttccagaagg
     1381 gcgaaataac ggtcgaaggc atatcggtcg gcgacccgaa gaccaacctg ccgaagctgc
     1441 gttcacgcat cggcatggtg ttccagaact tcgagctgtt cccgcacatg tcggtcaccg
     1501 agaacctgac cctcggccag gttaaggtgc tcggccgcag ccaggacgag gcgcgcgcca
     1561 agggcctaaa gctcctcgag cgcgtcgggc tgatggcgca caaggacaag ttccccggcc
     1621 agctctccgg cggccagcag cagcgcgtgg cgatcgcgcg cgctctctcg atggacccga
     1681 tcgcgatgct gttcgacgag cccacttcgg cgctcgatcc cgagatgatc aacgaggtgc
     1741 tcgacgtcat ggtcgcgctc gcccgcgagg gcatgaccat gatggtggtg acgcacgaga
     1801 tgggcttcgc ccgcaaggtc gcgcaccgcg taatcttcat ggacggcggc cgcatcgtcg
     1861 aggatgcgaa gaaggacgag ttcttcggca cgccgcgctc ggagcgcgcg cagcagttcc
     1921 tgtccaagat cctgcaccat tgacagaatc ggagcaatga agcgaagcct gatggcattc
     1981 gccgttccga tgctcgccgc gctccatgcg acggcgcaag acgagaaggc tgtaagcgag
     2041 ctcgcggcag aaacgaaagc gcatttcgca gggcagtgct cgcttgctcg cagcagcgct
     2101 tcctccgccg cgggatcgcg gcaaagagat caggcggtca tgctcgcccg gctgaactgc
     2161 gattgcctgc cggccgagat ggagcgcgtc gcctccgact tgagcgcggg caaggcgaac
     2221 gccacggcga cgccggcagc ctttctttcg cgggtaaaga cggcgttcaa cgcctgcgcg
     2281 gcacggatcg cgcgcgctga catagtggca agctgcgccg ccggggatgc gagcaagctc
     2341 ggagtggcaa acaagcccat ctactgcgga tgcctgtccg aaggactgaa agcgctcgat
     2401 gacgacacca tcgtgagcgc cgcggcccgc gtacaccgga actccgagga cagggtgcgg
     2461 gcaagatcca aaggcaggcc ggagcctgtc atcgctccgt cggcggtgga tagtctcgag
     2521 cagcgctgca aagagctcgc gggctgaccg gactcgctac ggctgtttct cgactttctc
     2581 cagcatcgcc tccgagatcc gatagtcggg ataagcgcgc ctgaactcgg cgagcgcctt
     2641 gtccgcttcc tcatgacgtc cctgccgccg caggtcggcg atgccctgca gccattgctc
     2701 cggcgaagcg ggggcgcgtg cttcgagcgc cgcggccttt tcccgcatga cgccttgcgc
     2761 ttcggctcgg agctgcggac tcgccgcggc atcggcgcgg gcgggggcag ccggagcgac
     2821 ttgccgtttt tccagatcat cgagcgcctc ggctttgcgc tgcgcgcgcg actctcgcgg
     2881 cggctccggc ttcggatcct gggctgcggg tgccggctcg tgtcgcgcgg gcgcttcggc
     2941 aacttccatg tccggacgct gacgctccac atggaccgtg actgcgacgg cgagaacgat
     3001 gatggcggcg gcagcgagcg ggacgtacca gcgccgccgc ccggccggcg caacgagcgg
     3061 cgcgggccgc gcttcgacgc cccggcgcgc ggcggcgagg attgcgtcgt cgagctgggg
     3121 cggcggctcc tcgcgcgaga gctcgcgata gcgccgggag accttgtcgt cgtcagtcct
     3181 catcgactgc agccttcagc ttcgccatgg cgtagcgcaa
//
LOCUS       DASUNT010000046         2410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_182215, whole genome shotgun sequence.
ACCESSION   DASUNT010000046 DASUNT010000000
VERSION     DASUNT010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182215"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VFX94_00925"
     CDS             <1..486
                     /locus_tag="VFX94_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766769.1"
                     /translation="EVSQDVIVNARPCVEAAVLPALDEKGDLPPGVHGAGWTEIESRF
                     GTGSSARMHAIATLKRLHDLAKRTGALKNFYVFGSFVSAVSEPRDVDVVLIMDAGFRL
                     ESCPPESRSLFSHAEAEARYDATVFWIREGMLPRAAMRDFLSAWQIRRDGALRGILEV
                     A"
     gene            483..692
                     /locus_tag="VFX94_00930"
     CDS             483..692
                     /locus_tag="VFX94_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766770.1"
                     /translation="MIRNDNELAVTRERVAKFERLLEELRKTARPEEWAELSSGYRLE
                     IERMQREILDYLVQPAPDPKRAKTA"
     gene            complement(694..927)
                     /locus_tag="VFX94_00935"
     CDS             complement(694..927)
                     /locus_tag="VFX94_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766771.1"
                     /translation="MAALESAKAQPMSAVEIDPSLIRALQARARKEKTTVERLVRRLI
                     ADSLQELEDYEAVKAFRARAGRTAVLADVKKRL"
     gene            1249..1521
                     /locus_tag="VFX94_00940"
     CDS             1249..1521
                     /locus_tag="VFX94_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5766772.1"
                     /translation="MAKARRNLGKEILEGIREIKRGEHGRVSNLPSVASIRESTGLSQ
                     SRFAELLGVSVRTLQEWEQGRRAPSGAARTLLLIAAINPRALRQVA"
     gene            complement(1522..>2410)
                     /locus_tag="VFX94_00945"
     CDS             complement(1522..>2410)
                     /locus_tag="VFX94_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX5766773.1"
                     /translation="KLGMSRASVKRDLEYMRSRFNAPIEYVRELNGYRFGKPRSGPRY
                     ELPGLWFNAAEVQALLTTLQLLTNLQPGLLDGQVKPVLERLRSILGKGDHSWEEVVKR
                     MRIFLPERRQGQAAHFSVVAAALLKRQRIRIKHYNRKEDRETEREVSPQRLVHYRDNW
                     YLDAYCHLREDLRSFAVDAIREAVLKDVPAKEVREAELDEYLGSGYGIFGGRKVQWAT
                     LKFSPEAARWVSAQNWHPKQRARVEKDGSYVLELPYAEDRELVMEILKYGADVEVLAP
                     AELRKRVAGALKLAAVRYD"
BASE COUNT          399 a          765 c          832 g          414 t
ORIGIN      
        1 gaggtgagcc aggatgtcat cgtaaacgct cggccgtgcg ttgaagcagc cgtgctaccc
       61 gctctcgatg agaaaggcga ccttccgccg ggcgtgcatg gcgcgggctg gactgagatc
      121 gagtcccggt tcgggaccgg aagtagcgcg cgcatgcacg cgatcgcgac actcaagcgc
      181 ctgcacgatc tcgccaagcg caccggtgcg ctgaagaact tctacgtgtt cggcagcttc
      241 gtttcggcag tctccgaacc ccgggacgtg gacgtagtgc tgatcatgga cgccggcttc
      301 aggctcgaga gttgcccgcc agagtcgcgc agcttgtttt cgcacgcgga ggcagaagcc
      361 cgctatgacg ccaccgtctt ctggatcagg gaagggatgc tgccgcgggc cgcgatgcgg
      421 gatttcctga gcgcatggca gatcaggcgg gacggggcgc ttcgtggcat attggaggtc
      481 gcatgatccg caatgacaac gagctcgccg tcacgcggga gcgcgtggcg aagttcgagc
      541 gcctgctcga ggagctgcgc aaaaccgcgc ggcccgagga atgggcggag ctcagctccg
      601 gctaccggtt ggagatcgag cgaatgcagc gcgagatcct ggactatctc gtccagccgg
      661 ccccggaccc caagcgcgcg aagacggcct gatctaaagg cgcttcttga cgtcggcgag
      721 caccgccgtg cggccggccc gggcacggaa cgccttgacc gcctcgtaat cctccagttc
      781 ctggagcgaa tcggcgatca ggcgccttac cagccgttct accgtcgtct tctccttgcg
      841 tgcgcgcgcc tgcaaggcgc gaatcagcga gggatcgatc tcgacggcag acatgggttg
      901 cgctttggcg gattccagcg cggccattct aacgcgcctc gtcgtcgagg acccagagcg
      961 cgaacagcac gatggtgggc aggagcgcga tgaggaggtc gagcgggatc atggcggcag
     1021 gctaacgccg gcgatggctc agcgcgtgag cctgcgctga tgaaatgatg cgcgtgccct
     1081 ggccggtata cggcaatgac gtatgatgct gaccttcgtt gaaacgaggc tcttttcgcg
     1141 cctgctcgcg cagtacttga ccggcgatga atacgcggtg ctacaacaag cgctggcacg
     1201 ctctccggaa cggggcagcc tcgttcccgg ctcaggcggc ggagatcgat ggctaaggcg
     1261 aggcgcaatc tgggcaagga gattctcgag ggcatccgcg agatcaagcg cggcgagcac
     1321 ggccgcgtgt cgaacctgcc gtcggtcgcc tcgatccgcg aaagcaccgg gctgtcgcaa
     1381 tcgcgcttcg cggagctgct cggcgtgtcg gtgcgcacgc tgcaggaatg ggagcagggc
     1441 cgccgcgcgc cgagcggcgc cgcgcgcacg ctgctgctca tcgcggcgat aaatccgcgc
     1501 gccctcaggc aagtggcgta actagtcgta gcgcacggcc gcgagcttga gggcgccggc
     1561 gacgcgcttg cgcagctccg ccggcgcgag cacttccacg tcggcgccgt acttgaggat
     1621 ttccatcacc agctcgcggt cttcggcata gggcagctcg aggacatagc tgccgtcctt
     1681 ctcgacgcgg gcgcgctgct tggggtgcca gttctgggcc gatacccagc gcgcggcctc
     1741 gggcgagaac ttgagcgtcg cccactggac cttgcggccg ccgaagatgc cgtagccgct
     1801 accgagatat tcatccagtt ccgcctcgcg tacctccttc gcaggtacgt ccttcagaac
     1861 cgcttcgcgg atcgcgtcga cggcgaagct cctcaggtct tcgcgcaggt ggcagtaagc
     1921 gtcgaggtac cagttgtcgc ggtagtgcac caggcgctga ggcgacacct cgcgctcggt
     1981 ctcgcggtct tccttgcggt tgtagtgctt gatccggatg cgctgccgct tgaggagcgc
     2041 cgccgcgacg acgctgaaat gagcggcttg cccctggcgc cgctcgggca ggaagatgcg
     2101 catgcgcttg acgacctctt cccatgagtg gtcgcccttg ccgaggatcg agcgcaggcg
     2161 ctcgagcacc ggcttcacct ggccgtcgag caggccgggc tgcaggttgg tgaggagctg
     2221 cagggtggtg aggagcgcct gcacctcggc ggcgttgaac cacaggcccg gcagctcgta
     2281 acgcggcccc gagcgcggct tgccgaagcg gtagccgttc agctcgcgca cgtattcgat
     2341 cggcgcgtta aaccggctgc gcatgtactc gaggtcgcgc ttcacgctcg cgcgcgacat
     2401 gccgagcttt
//
LOCUS       DASUNT010000047         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1146744, whole genome shotgun sequence.
ACCESSION   DASUNT010000047 DASUNT010000000
VERSION     DASUNT010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146744"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2222)
                     /locus_tag="VFX94_00950"
     CDS             complement(<1..>2222)
                     /locus_tag="VFX94_00950"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX5766774.1"
                     /translation="VLVATASLELGIDIGHVDLVCQISSPHRIATFLQRVGRSGHTIK
                     GVPKGRIFPLTRDDLIECAAMVRAVHDGELDRVAVPDKPLDVLGQQIVAETSAQSEWD
                     VDALLALFRQAYPYRTLEKERFMDVVEMLARGYATRRGRRGALIHYDSLNKELRSRKG
                     SRLSAIVNGGAIPEVFDYRVLLEPEGHFIGTLNEDFAIESLPGDVFQLGNTSWRILRI
                     GNGVVRVADAQGQPPSMPFWLGEAPARSDEMSAAVSALRAAADAQLPGPDEPRKEGEL
                     AAAIELLEKEYLLGRTAAEQIAAYLGEAKRSLGAVPTTDTIALERFFDESGGMQLVLH
                     APFGNRVNRAWGLALRKKFCQGFNFELQAAATDEGVILSLGELHSFPLEEVFHYLHPN
                     TVRETLMQAVLASPLFETRWRWSTTLALAVPRNRGGARIPNQLQRMYAEDLLQAVFPD
                     AVACQDNMQGAREIPDHPLVSQALRDALEEAVDLKGLETQLKRLYSGELRTIVRDTPE
                     PSVLSHELLNSAVYTFLDDAPLEERRTRAVYTRRATEVRSSDDLGALDPAAIQRVREE
                     AWPVANTPDEMYDALMVAGYIKEGELAGHWPALLEQLGGRAIKRGDAWFALERKDDDE
                     VEITASRLEVLGPISSSETPVLLSLEAQGRILRGQFSSNELQWCDRRLLARIHRYTLN
                     RLRAEIEPVSAAEFMRFLLHWQHVAGEDQMKGAEGLMAVIEQLEGFELAAAAWENDVL
                     "
BASE COUNT          356 a          752 c          717 g          397 t
ORIGIN      
        1 cagcacgtcg ttttcccagg ccgccgcggc aagctcaaag ccttcgagct gctcgatgac
       61 cgccatgagg ccttcggcgc ccttcatctg atcctcgccg gcgacgtgct gccagtggag
      121 cagaaagcgc atgaactcgg cggcgcttac cggttctatc tccgccctta gtctattcag
      181 ggtgtagcga tggatgcgcg cgagcagccg gcggtcgcac cattgcagct cgttcgacga
      241 aaactgcccg cgcagaatgc ggccctgcgc ttcgagcgag agcaacaccg gtgtctcgct
      301 ggagctgatt ggccccagga cctcgagccg gctcgcggta atctccacct cgtcgtcgtc
      361 cttgcgttcg agcgcgaacc aggcgtcgcc ccgcttgatg gctcttccac ccaattgctc
      421 cagcagcgcc ggccaatgtc cggccagttc gccttccttg atgtacccgg ccaccatcag
      481 cgcgtcgtac atctcgtccg gcgtattggc caccggccac gcttcttcgc gcacgcgctg
      541 aatcgccgcc gggtcgagcg cgccgaggtc gtcggagctg cgaacctcgg tcgcgcggcg
      601 cgtgtagacg gctcgcgtgc ggcgctcctc gagcggagcg tcgtcgagga aggtgtagac
      661 cgcggagttc agcagctcgt ggctcagcac cgagggctcc ggtgtgtcgc gcacgatcgt
      721 ccggagttcc ccggaataaa gtctcttcaa ttgagtttcc aggcccttca gatctactgc
      781 ctcctcgagc gcgtcgcgca gcgcctggct gacgagcgga tggtctggaa tctcgcgcgc
      841 gccctgcatg ttgtcctggc acgccacggc gtccggaaat acggcctgga gcaagtcctc
      901 cgcatacatg cgctgcagct ggttcgggat gcgcgcgccg ccgcggttgc gcggcacggc
      961 gagcgccagc gtggtgctcc agcgccagcg tgtctcgaag agcggcgagg cgaggaccgc
     1021 ctgcatcagc gtctcgcgca cggtgttcgg atggaggtaa tggaacacct cttcgagcgg
     1081 gaacgaatgc agctcaccca gcgaaaggat gaccccctcg tcggtcgcgg ccgcctgcag
     1141 ctcgaagttg aagccctggc agaacttctt gcgcaacgcg agcccccacg cgcggttgac
     1201 acggttgccg aacggcgcgt gcagcacgag ctgcatgccg cccgactcgt cgaagaagcg
     1261 ctcgagggcg atggtgtcgg tcgtcggcac ggcgccgagg ctgcgcttcg cttcgcccag
     1321 ataggcggcg atctgctccg cggccgttct cccgagtaga tactcttttt cgagaagctc
     1381 gatggcggcc gcaagctcgc cttctttacg cggctcatcg ggcccgggca actgggcatc
     1441 cgcggcggcg cgcaatgcag acaccgcagc gctcatctcg tcggaacgcg ctggcgcttc
     1501 gccgagccag aacggcatcg acggcggctg gccctgcgcg tcggccaccc gcaccacccc
     1561 attgccgatg cgcaggatgc gccatgacgt attgccgagc tggaacacgt ccccgggcag
     1621 cgactcgatg gcgaagtctt cgttcagcgt gccgatgaaa tgcccttcgg gctcgaggag
     1681 cacgcggtag tcgaacacct cgggaatcgc gccgccgttg acgatggcgc tgaggcgcga
     1741 tccctttctg cttctcagct ctttattgag actgtcgtaa tggatcagtg ccccgcggcg
     1801 cccgcggcgg gtcgcgtatc cgcgcgcaag catttctacc acgtccatga atcgctcttt
     1861 ctcgagcgtc cggtacgggt aagcctgcct gaagagcgca agaagcgcgt caacgtccca
     1921 ctccgattgc gctgaagttt cggcgacgat ctgctgcccg agcacgtcga gcggcttgtc
     1981 gggcaccgct acccggtcga gctcgccgtc gtgcaccgcg cgcaccatgg cggcgcactc
     2041 gatgagatcg tcgcgcgtga gcgggaagat gcggcccttc ggcacgccct tgatcgtgtg
     2101 ccccgagcgt cccacccgct gcaggaaggt ggcgatccgg tgcggggacg agatctggca
     2161 cacgaggtcg acgtgaccga tgtcgatgcc gagctcgagc gacgcagtag ccaccagcac
     2221 tt
//
LOCUS       DASUNT010000048         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_708307, whole genome shotgun sequence.
ACCESSION   DASUNT010000048 DASUNT010000000
VERSION     DASUNT010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708307"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1463)
                     /locus_tag="VFX94_00955"
     CDS             complement(162..1463)
                     /locus_tag="VFX94_00955"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX5766775.1"
                     /translation="MTARNVVVIGTQWGDEGKGKIVDWLTDRAQGVVRFQGGHNAGHT
                     LVIGGKKTVLHLIPSGILRENVDCFIGNGVVVSPQALVAEMDELEAAGVRGVVARLSV
                     SEACPLILPYHAALDHAREAAKGERKIGTTGRGIGPAYEDKVARRAIRLQDLFEPARF
                     AQKLRELLDFHNFVLHEYYKQPKLDYKTILDESLGLAPRLAPLIADVPRSLYHANRAG
                     KNLLFEGAQGSLLDIDHGTYPFVTSSNCVAGAAAAGAGIGPHHLHYVLGITKAYTTRV
                     GGGPFPTELSDDVGERLRQRGQEFGATTGRPRRTGWFDAAALKRSIQLNGLSGLCITK
                     LDVLDGVESVKICVGYELEGSFSEILPVGAEELERCHPRYDELPGWMESTVGIRAYEA
                     LPQAARAYLGRIEALAGVPIDLISTGPDRDETIVRRHPYDV"
     gene            complement(1460..>2096)
                     /locus_tag="VFX94_00960"
     CDS             complement(1460..>2096)
                     /locus_tag="VFX94_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX5766776.1"
                     /translation="SVLHTRPAAPGATREPIQIGAEMYGEAGVAADVEMLELLCSALE
                     LAGVRKARVDIGHVAVFRAIAHEAGVDAEREAELFEALQRKDTPEIKNLSRSLQSAYR
                     EALLMLPGLYGGAEIIDEAQKKLPPLPEVKKALATLGRLAKACKFPAGFDLAELRGYH
                     YHSGVVFDAYCDGAAGAVARGGRYDEVGKAFGRARPATGFSIDLRTLASVS"
BASE COUNT          313 a          713 c          720 g          350 t
ORIGIN      
        1 accatcgctc tgatcgcggc gtgccgggcg cgcgcctcgt cgatatcgcg gtgcatcgcg
       61 cagccatttt gcgaccgtgc gcgcccgggc gctgtcgcgc cgctccgaaa tccgcgtggg
      121 cggggcgtga caacgcgtca ctacgagccg acaagcgtta ttcagacatc gtacgggtgg
      181 cggcgcacaa tggtctcgtc gcggtccggg ccggtggaga tcaggtcgat cggaacgcct
      241 gcgagcgcct cgatgcgccc gagatacgcg cgcgccgcct ggggcagcgc ctcgtaggcg
      301 cgaatgccga cggtgctttc catccacccg ggaagctcgt cgtagcgcgg gtggcagcgc
      361 tcgagctcct cggcgcccac gggcaggatc tcggagaagc tgccctccag ctcgtagccg
      421 acgcagatct ttaccgattc gaccccgtcg agcacgtcga gcttggtaat gcacaggccc
      481 gaaaggccgt ttagctgaat cgagcgcttg agcgccgcgg cgtcgaacca tccggtgcgc
      541 cgcggccggc cggtggtggc gccgaattcc tgcccgcgct ggcgcaggcg ctcgccgaca
      601 tcgtcggaca gctcggtcgg gaacggccct ccgccaactc gcgtcgtata cgccttggtg
      661 attccgagta catagtgcag atggtgcggc ccgattcccg cgcccgctgc ggcggcgccg
      721 gcgacgcagt tcgacgacgt gacgaacgga taggtgccgt ggtcgatgtc caggagcgat
      781 ccctgcgcgc cctcgaaaag caggttcttg ccggcgcggt tcgcgtggta gagcgagcgc
      841 ggcacgtcgg caatgagcgg cgcaagccgc ggcgcgaggc ccaggctctc atccagaatc
      901 gtcttgtagt cgagcttggg ctgcttgtag tactcgtgaa gcacgaagtt atgaaagtcg
      961 agcagctcgc gcagcttttg cgcgaagcgg gccggctcga agagatcctg cagccggatc
     1021 gcacgccggg cgaccttgtc ctcgtaggcc gggccgatgc cccggccggt cgtgccgatc
     1081 ttgcgctcgc ccttggcggc ttcgcgcgca tggtcgagcg ccgcgtggta gggcaggatc
     1141 agcgggcacg cttcgctgac cgaaagccgt gccaccacgc cgcgcacgcc ggcagcctcg
     1201 agctcgtcca tctcggcgac gagcgcctgc ggcgacacca cgacgccatt accgatgaag
     1261 cagtcgacgt tctcgcgcag gatgcccgag gggatgaggt gcagcaccgt tttcttgccg
     1321 ccgatgacga gcgtgtggcc cgcgttgtgg ccgccctgga agcgcaccac cccctgagcg
     1381 cggtcggtga gccagtcgac gatcttgccc ttgccttcgt cgccccactg ggtgccgatg
     1441 acgacgacgt ttctcgcggt catgaaacgg aagcgagggt tctcaagtcg atggaaaagc
     1501 cggtggccgg ccgcgcacgg ccgaaagctt tcccgacttc gtcgtaacgg ccgccgcgcg
     1561 ccaccgcccc ggcggcgccg tcgcaatacg catcgaacac cactccggaa tggtagtggt
     1621 agccgcgcag ctcggcgagg tcgaacccgg cggggaactt gcacgccttg gcgagcctgc
     1681 cgagcgtggc gagcgccttc ttcacctcgg ggagcggcgg gagctttttt tgcgcctcgt
     1741 cgatgatttc cgcgccgccg tagaggccgg gcagcatgag cagcgcctcg cgatacgcgc
     1801 tttgcagtga acggctgagg ttttttattt ccggcgtgtc cttgcgctgc aacgcctcga
     1861 agagctcggc ttcgcgctcg gcgtcgaccc ccgcctcgtg cgcgatcgcg cggaacacgg
     1921 ccacgtggcc gatgtccacg cgcgccttgc gcacgccggc gagctcgagc gcgctgcaga
     1981 gcagctcgag catttccacg tcggccgcca cgcccgcctc gccgtacatt tcggcgccga
     2041 tctggatcgg ctcgcgcgtg gctcccggcg cggccgggcg ggtatgcagc acgctg
//
LOCUS       DASUNT010000049         2325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_269811, whole genome shotgun sequence.
ACCESSION   DASUNT010000049 DASUNT010000000
VERSION     DASUNT010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269811"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..748
                     /locus_tag="VFX94_00965"
     CDS             95..748
                     /locus_tag="VFX94_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX5766777.1"
                     /translation="MAAIADTLQLVRTRIAARPGVTLLAVSKGQDVERIREAIAAGQK
                     AFGENYVQEAVRKMPQLPALEWHLIGPLQSNKTRVAAERFDWVQTIANEKVARRLSEQ
                     RPAHLAPLNVLIQVNVSAEKSKSGIALHEVRDLARAIAAMPRLRLRGLMAIPEPGAAR
                     SRYQEVKRTFDSLKEEFAFDTLSMGMSEDLDIALEEGATLVRIGTAIFGPRLQKSAA"
     gene            745..1557
                     /gene="proC"
                     /locus_tag="VFX94_00970"
     CDS             745..1557
                     /gene="proC"
                     /locus_tag="VFX94_00970"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX5766778.1"
                     /translation="MRIAFLGGGNMASALIGGLIAKGADARSIAVVEVSAAARDRLGA
                     RFPVHIASAPDAALQRCDVLVLAVKPQDAKAAVAPISGTVQGKLLISIAAGVTLETLS
                     RWLGRHRKLVRCMPNTPALIGAGIAGLYALPEVSAQERAAAQGILAAVGEVVWVEEER
                     LLDPVTAVSASGPAYVFWFIEQLAATAEKLGLSRDTSMKLALHTVLGAAKLAASSGDP
                     PATLRRNVTSKGGTTEAALKVFDEEKLAERFVRAVEAASRRATELGAEQGKG"
     gene            1559..2110
                     /locus_tag="VFX94_00975"
     CDS             1559..2110
                     /locus_tag="VFX94_00975"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX5766779.1"
                     /translation="MFTQIGVFLVDAVASFFVFMLLARFHFQWLRVPFRNPVGEFVLA
                     TTNWIVVPARSAIPGLAGLDLASLFLAWVVQGLGLWLQAAILGAEPGVGMIAAVALVD
                     LVRYSLYILVFAVIVQAVLSWVNPYTPLAPAFDTITRPFLRPLRRYVPPVGNVDLTPL
                     ILLVILYVVLIAVNHLRVAAATV"
     gene            2107..>2325
                     /locus_tag="VFX94_00980"
     CDS             2107..>2325
                     /locus_tag="VFX94_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX5766780.1"
                     /translation="MIVELHVQPGASRTEFAGKHGERLKVRLQARAVDGKANDALIEF
                     LAAHFGVPRRNVHIVSGLKSRQKRVRILP"
BASE COUNT          388 a          724 c          790 g          423 t
ORIGIN      
        1 cttgttcttc acgacgaacg cgagcaactc ggtgagatcc attagaatcc tccctggatc
       61 ggcgaagtgt ttttcttcgc tggttcaacg cattatggcg gcaattgcag acaccctgca
      121 acttgtgagg acgcgcatcg cagctcgtcc gggagtgact ttgttggcag tttccaaagg
      181 acaagacgtc gaacgcatca gggaagcgat cgcggccggg cagaaagcct tcggtgagaa
      241 ttacgtccag gaggcggtgc gaaaaatgcc gcagttgccg gcgctggaat ggcacctcat
      301 cgggccgctc cagtccaaca agacgcgcgt ggcggcggag cgcttcgact gggtgcaaac
      361 gattgcgaac gagaaggtcg cgcggcgcct ctcggagcag cgcccggcgc atcttgcgcc
      421 gctcaacgtg ctgatacagg tcaacgtgtc ggcagagaag agcaagagcg gcattgcact
      481 tcacgaagtg agggatctgg caagagccat cgcagcgatg ccgcggctgc gcctgcgcgg
      541 tttgatggcg atccccgaac ccggcgcggc ccgctcgcgc taccaggaag tgaagaggac
      601 gttcgattcc ctgaaggagg aattcgcgtt cgatacgctt tcaatgggca tgtccgagga
      661 cctcgacatc gcgctcgagg aaggcgcaac cctggtgcgc atcgggaccg cgatcttcgg
      721 tccccggctg cagaaaagcg cggcgtgagg atcgcgttcc tcggcggcgg gaacatggcg
      781 agcgcgctga tcggcggcct gatcgccaaa ggcgccgacg cgcgctcgat cgcggtcgtg
      841 gaggttagcg cggcggcgcg cgaccgcctc ggcgcgcgct tcccggttca cattgcgtcc
      901 gcgccggatg ccgcgctgca gcgctgcgac gtgctggtgc tcgcggtcaa gcctcaggat
      961 gcgaaagccg ccgtggcgcc gatttccgga actgttcaag gcaagcttct catcagcatc
     1021 gccgcgggtg ttacgctgga gacgctgtcg cgctggctcg gcaggcaccg caagctcgtg
     1081 cgctgcatgc cgaatacccc cgcgctgatc ggagcgggaa tcgctggcct ctatgcgctg
     1141 ccggaggtga gcgctcagga aagagccgcg gcgcaaggca tactggccgc agtcggcgag
     1201 gtggtgtggg tggaagagga acggctgctc gatcccgtga ccgcggtttc ggcgagcggg
     1261 ccggcttacg tgttctggtt catcgagcag ctcgcggcga cggcagagaa gctgggcttg
     1321 agcagggaca cttccatgaa acttgcgctg cacaccgtgc ttggcgcggc caagcttgcc
     1381 gcctcgagcg gcgacccgcc cgccactctg cgcaggaatg tgacctccaa aggcggcacc
     1441 accgaagcgg cactcaaggt cttcgacgag gagaagcttg ccgagcggtt cgtgcgcgca
     1501 gtggaagcgg caagccgccg cgccacggag ctcggcgcgg agcaggggaa gggctgacat
     1561 gttcacccag atcggcgtct tcctggtgga cgcggtggcg agcttcttcg tcttcatgct
     1621 gcttgcgcgg tttcatttcc agtggctgcg ggtgccattt cgcaatccgg tcggcgagtt
     1681 cgtgctggcg accaccaact ggatcgtcgt gccggcgagg agcgcgatcc cgggacttgc
     1741 cggcctggac ctcgccagcc tgttcctcgc ctgggtggtc cagggcctag gcttgtggct
     1801 gcaggcggct atccttggcg ccgagcctgg agtcggcatg atcgctgcag tcgcgctcgt
     1861 cgaccttgtg cgctacagcc tgtacatcct ggtgttcgcg gtaatcgtgc aggcagtgct
     1921 gtcgtgggtg aatccctata cgccgctcgc acccgcgttc gacactatca cccggccgtt
     1981 cttgcggccc ttgcgccgct acgtgccgcc ggtaggaaac gtggatctca cgccgcttat
     2041 tcttctcgtg atcctgtatg tagttctcat cgcggtgaac cacctccgcg tcgccgcggc
     2101 cacggtgtga tcgtcgagct ccacgtgcag cccggcgcct cgcgcaccga gttcgccggc
     2161 aagcacggcg agcggctgaa ggtcaggctt caggcgcgcg cggtcgatgg aaaggcgaac
     2221 gacgccctga tcgagttcct cgccgcgcat ttcggggtgc cgcggcgcaa cgtgcacatc
     2281 gtttccgggc tcaagtcccg gcagaagcgg gtccggatcc ttccc
//
LOCUS       DASUNT010000050         2055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_532883, whole genome shotgun sequence.
ACCESSION   DASUNT010000050 DASUNT010000000
VERSION     DASUNT010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532883"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /locus_tag="VFX94_00985"
     CDS             complement(<1..1458)
                     /locus_tag="VFX94_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5766781.1"
                     /translation="MFALLPRDFHPLLERWWASRFEEPTAAQLEGWRSIREGRHTLIA
                     APTGSGKTLAAFLTSIDDLLEEGMEQGGLPDEVRVIYVSPLKALSADIHKNLAVPRRE
                     IHALAPVRITAAVRSGDTPQPERAAMLRTPPHILVTTPESLYLLLTAERSRAMLKTAR
                     VVIVDEIHAVLQSRRGAHLALSLERLDHVCGRKLQRIGLSATQKPIAEVARFLTQEDV
                     AVVDKGHKRQLDLALEVPSSPLEAVMSHEVWKEIYDRLVALIESHRTTLITVNTRRLA
                     ERMAHQLSERLGAEYVAAHHGSLAKEARLDAEERLRSGKLRVLVATASLELGIDIGHV
                     DLVCQISSPHRIATFLQRVGRSGHTVKGVPKGRIFPLTRDDLVECAAMVRAVHDGELD
                     RVAVPDKPLDVLAQQIVAEVSAGEWDEDRLLAVFTQAYPYRALQKDEFMDVVEMLARG
                     YATRRGRRGALIHHDSLNKKLRSRKGSRLSAIVNGG"
     gene            1498..>2055
                     /locus_tag="VFX94_00990"
     CDS             1498..>2055
                     /locus_tag="VFX94_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX5766782.1"
                     /translation="MNKTRMLTLEQLKGMVAQGEIETVLAGFTDHYGRLMGKRFDAEM
                     FVDEIAKHGGHACDYLLTVDMEMEPVPGYSFANWELGYGDVHMVPDFDTLRLASWLDK
                     TAFVLCDVKSDKTHGYVDVAPRSILRRQIEAAKKLGYAGYAGTELEHYLFQTSYRQAA
                     QQDYRNLEPAGWYLEDYHMLQGMRTE"
BASE COUNT          363 a          669 c          677 g          346 t
ORIGIN      
        1 gcccccgtta acgatggcgc tcagccgcga tccctttctg cttctcagct ttttgttaag
       61 actgtcatga tgaatcagtg cgccgcggcg cccgcgccgg gtcgcgtacc cacgcgcgag
      121 catctccacc acatccatga actcgtcttt ttggagcgcc cggtacggat acgcctgagt
      181 gaagacagca aggaggcgat cctcgtccca ttccccggcc gacacctccg cgacgatctg
      241 ctgcgccagc acgtcgagcg gcttgtcggg cacggctacg cgatccagct cgccgtcgtg
      301 caccgcgcgc accatggcgg cgcactcgac gaggtcatcg cgcgtgagcg ggaagatgcg
      361 gcctttcggc acgcccttga cggtgtggcc cgagcgcccc acccgctgca ggaaggtggc
      421 gatgcggtgc ggcgaggaga tctggcacac gagatcgacg tggccgatgt cgatgccgag
      481 ctcgagcgac gcggtggcca ccagcactct gagctttccg cttctcagcc gctcctcggc
      541 atccagccgc gcttccttcg ccaggctacc gtggtgcgcg gccacgtatt ctgcgccaag
      601 gcgctccgag agctgatgcg ccatgcgctc ggccaggcgt cgggtgttga cggtgatcag
      661 cgtcgtgcga tgcgactcga tcagcgccac gaggcggtcg tagatttcct tccatacctc
      721 gtgcgacatc accgcctcga gcggcgagct cggcacttcg agcgccaggt ccagctgccg
      781 tttatggccc ttgtcgacga cggcaacgtc ttcctgagtc aagaaacgtg caacttcggc
      841 gatcggcttc tgcgtggccg acagcccgat gcgctgaagc ttgcgcccgc agacgtggtc
      901 caggcgctcg agcgacagcg cgaggtgcgc accgcggcgg gactgcaaga ccgcgtggat
      961 ctcgtccacg atcaccaccc gggccgtctt cagcatcgcg cgcgagcgct cggcggtcag
     1021 gagcagataa agcgactccg gcgtggttac taatatatga ggcggcgtgc ggagcatggc
     1081 cgcgcgctcg ggctgcggcg tatccccgct ccggacagcg gctgtgatcc tcacgggggc
     1141 gagggcatgg atctcccgcc gcggcaccgc caggttctta tgaatgtcgg ccgagagcgc
     1201 cttcagcggc gagacataga tgacgcgcac ctcgtcgggc aggccgccct gctccatccc
     1261 ctcctcgagc agatcgtcga tggaggtgag gaaggcggcg agcgtcttgc cggagccggt
     1321 cggagcggcg ataagcgtgt ggcggccttc gcggatcgag cgccaacctt cgagctgcgc
     1381 ggcggtcggc tcctcgaagc ggctcgccca ccagcgttcc agtaagggat gaaaatcgcg
     1441 gggtagcaag gcgaacattt tgacattaga ttccggggtt cattcgagaa aaacgcgatg
     1501 aacaagacgc gcatgctgac gctcgagcag ttgaagggca tggtggcgca gggcgagatc
     1561 gagaccgtgc tcgccggctt caccgaccac tatggccggc tgatgggcaa gcgcttcgac
     1621 gccgagatgt tcgtcgacga gatcgccaag cacggcggcc acgcctgcga ctatctcctc
     1681 acggtcgaca tggagatgga gccggtgccg ggctacagct tcgccaactg ggagctcggc
     1741 tacggcgacg tacacatggt cccggacttc gacacgctgc ggctcgcaag ctggctggac
     1801 aagacggcgt tcgtgctgtg cgacgtgaaa agcgacaaga cgcacggcta cgtcgacgtt
     1861 gcgccgcgct cgatcctgcg ccggcagatc gaagcggcga aaaagctcgg ctatgccggc
     1921 tatgcgggca ccgagctcga gcactacctc ttccagacga gctaccgcca ggcggcccag
     1981 caggattatc gaaatctgga gcccgcgggc tggtacctcg aggactacca catgctgcag
     2041 ggcatgcgca ccgag
//
LOCUS       DASUNT010000051         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_270152, whole genome shotgun sequence.
ACCESSION   DASUNT010000051 DASUNT010000000
VERSION     DASUNT010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270152"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFX94_00995"
     CDS             <1..495
                     /locus_tag="VFX94_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5766783.1"
                     /translation="IPVDGSRNSEFAVRHVVRQFMNNTAMEIHLLNVQQPLSSYVTRF
                     VSRKNVRDYHHDEAEKALQPARQILDSLGIPYAVHAELGPRAETITDAAKRLRCDHIV
                     MSAARKNSLTRLVESSTTNRVLELTPVPVEIIAGDSVSKWERYGIPASLGTAIALLLA
                     AAAD"
     gene            complement(515..1105)
                     /locus_tag="VFX94_01000"
     CDS             complement(515..1105)
                     /locus_tag="VFX94_01000"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEX5766784.1"
                     /translation="MRLAALVALALLAGCFSPGARQPQRFYVLEDPAKSYTAKSTRAS
                     TLLVAPTTAAGFYDTQNIAYSRSPGTRAYYHYHSWTELPARRIGELLGARLEKSGGFR
                     MVAGIGSAVRGDLVLSTHLAELYHDAASQPGVVRITLGAELTDPMKRTIVSRKLFNAS
                     APASSFDAPGAVHGFNQALASLLDEVSAWVDAEAPR"
     gene            complement(1102..2046)
                     /locus_tag="VFX94_01005"
     CDS             complement(1102..2046)
                     /locus_tag="VFX94_01005"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5766785.1"
                     /translation="MEPEAKYTVVGTAVLVLIAMVAAALLWLRTTGEGGDERTYKIYF
                     ERQSLEGLQPRSEVRMRGIRVGSVTGFRISSWRPGAVEVMVRVDGAAPVRQSTRAVVE
                     RHLVTGIASILLVNLNEESPPLTQAPQGEVYPVIAEGESQLEQVSQSLQELALRATET
                     MQRVSTTLSPENQRAFTALLENLNRLAHNAERGIAGLDRTVASVGAAADEVRVLTRAM
                     SEDAGRLTARYEKLGEEATVAAREAAAAMRQVSADVARISQRVDALLASGDVELRITA
                     QELRATADALGVVARRLNDPRSVLWGPPEGGLGPGEAR"
     gene            complement(2046..>2844)
                     /locus_tag="VFX94_01010"
     CDS             complement(2046..>2844)
                     /locus_tag="VFX94_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5766786.1"
                     /translation="GGVPAPARVMDPILEVESIVTRFGAEKVHDGVSFSVGRGEVVAL
                     IGGSGTGKSVLVKEIIGLMRPAAGRIRLLGTDVWAASPGELNALRRRFGMLFQDGALF
                     SSLTVAENIAVPFRENTDLPMELIAPLCGFKLALVGLPPDAGKKMPAELSGGMRKRAA
                     LARALALEPEILFLDEPTSGLDPVGARAFDSLVRLLSDSLGLTVFIVTHDLDTLLSII
                     DRTIVLSKGCVIADGPVSAVMRSPDPWIREYFSARSPLAPAAAGAPT"
BASE COUNT          453 a          976 c          996 g          419 t
ORIGIN      
        1 atcccggtcg acggctcgcg caacagcgag ttcgcggtgc ggcatgtggt gcggcagttc
       61 atgaacaaca ccgcgatgga gatccacctt ctgaacgtgc agcagccgct aagcagctac
      121 gtcacgcgct tcgtcagccg caagaacgtg cgcgactacc atcacgacga ggccgagaag
      181 gctctgcaac cggcgcgcca gatcctcgac agcctcggca tcccctatgc cgtgcacgcc
      241 gagctcggcc cgcgcgcgga gacgatcacc gacgcggcca agcggctgcg ctgcgaccac
      301 atcgtgatga gcgcggcgcg caagaactcg ctcacccgct tggtggagag ctcgaccacc
      361 aaccgggtgc tggagctcac cccggtgccg gtcgagatca tcgccggcga ctccgtatcg
      421 aagtgggagc gctatggcat cccggcgagc ctcggcaccg cgatcgcgct gctcctcgcg
      481 gccgcggcgg attgatggtg aaccggagcg cctgctagcg cggcgcctcg gcgtccaccc
      541 aggcgctcac ctcgtcgagc agcgaggcga gcgcctggtt gaatccgtgc accgcgccgg
      601 gtgcgtcgaa gctcgacgcc ggcgccgacg cgttgaagag cttgcgcgag acgatcgtgc
      661 gcttcatcgg atcggtgagc tcggcgccga gcgtgattcg cacgacgccc ggctgcgaag
      721 cggcgtcgtg gtagagctcg gcgaggtgcg tgctcagcac gaggtcgccg cgcaccgcac
      781 taccgatgcc ggccaccatg cggaagccgc cgcttttctc caggcgcgcg ccgagaagct
      841 caccgatcct gcgcgccggc agctccgtcc agctgtggta gtggtagtag gcccgcgtcc
      901 ccggcgagcg gctgtatgcg atgttttgcg tgtcgtagaa cccggcggcg gtggtcggcg
      961 cgacaagcaa ggtggaggcg cgggtgcttt tcgccgtgta gctctttgcg gggtcttcaa
     1021 gaacgtagaa tcgctgcggc tggcgggcgc cgggcgaaaa gcaccccgcg agaagcgcca
     1081 aggcgacaag cgccgcaagc ctcatcgagc ctctccgggg ccgaggcctc cctccggcgg
     1141 accccagagc acgctgcgcg gatcgttgag cctgcgcgcg accacgccga gggcgtcggc
     1201 cgtggcgcgc aactcctgcg cggtaatgcg aagctccaca tcgccgctcg cgagaagcgc
     1261 atccacgcgc tgcgagatgc gcgcaacatc ggcgctgacc tgccgcatgg cggcggcggc
     1321 ttctcgtgcc gcgacggtag cctcctcgcc gagcttctcg tagcgcgcag tgagccggcc
     1381 cgcgtcctcg ctcatagcgc gggtcaggac gcgcacttcg tcggcggccg cgcccaccga
     1441 cgcgacggtg cgatccagtc ccgcgatgcc gcgctcggcg ttgtgcgcga ggcggttcag
     1501 gttctcgagg agcgctgtga acgcgcgctg gttttcgggc gaaagcgtcg tgctgacgcg
     1561 ctgcatggtc tcggtcgcgc gcagcgcgag ctcctgcagc gactgcgaaa cctgctctag
     1621 ctgggactcc ccttcggcga tcaccggata cacctccccc tgaggcgcct gggtaagcgg
     1681 tgggctctcc tcgttcaggt tgacgagcag gatgctcgcg atgccggtga cgaggtggcg
     1741 ctcgaccacg gcgcgcgtgc tctgccgcac cggcgcggcg ccgtcgacgc gcaccatgac
     1801 ctcaaccgcg ccggggcgcc agctggagat gcgaaagccg gtgaccgaac cgacgcggat
     1861 gcccctcatg cgcacctcgc tgcgcggttg caggccttcg agcgactggc gctcgaaata
     1921 gatcttgtag gttcgctcgt cgccgccctc gccggtggtg cgcagccaga gcaaggcggc
     1981 cgccaccatg gcgatcagga cgaggaccgc ggtgccgacc actgtgtatt tcgcttccgg
     2041 ttccatcagg tcggcgcgcc ggcggcggcc ggcgcaagcg gcgagcgcgc ggagaagtat
     2101 tcacggatcc acggatcggg cgagcgcatc acggcggaca ccgggccgtc ggcgattacg
     2161 cagcccttcg acagcacgat cgtgcggtcg atgatcgaaa gcagcgtatc gaggtcgtgc
     2221 gtgacgatga acaccgtgag cccgaggctg tcggagagca ggcgcacgag gctgtcgaac
     2281 gcgcgcgcgc cgaccgggtc gagccccgaa gtcggctcgt cgagaaatag gatctccggc
     2341 tcgagcgcga gcgcgcgggc gagcgccgcc cgcttgcgca tgccgccgga aagctcggcc
     2401 ggcatctttt tccccgcgtc gggcggcagg ccgaccagcg cgagcttgaa gccgcagagc
     2461 ggtgcgatga gctccatcgg caggtcggta ttctcgcgaa acggcaccgc gatgttttcc
     2521 gccacggtga gcgaggaaaa gagcgcgccg tcctggaaga gcatgccgaa gcgccggcgc
     2581 agcgcgttga gctcgccggg gcttgccgcc cagacgtcgg tgccgagcag gcggatgcgg
     2641 ccggccgccg gacgcatgag gccgatgatc tccttgacga gcaccgactt cccggtgccg
     2701 ctgccgccga tgagcgccac gacctcgccg cggccgacgc tgaagctgac cccgtcgtgg
     2761 accttctcgg ctccgaaccg ggtgacgatc gattcgacct cgaggatcgg gtccatcaca
     2821 cgtgcaggcg ctggaacacc accg
//
LOCUS       DASUNT010000052         3581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_884098, whole genome shotgun sequence.
ACCESSION   DASUNT010000052 DASUNT010000000
VERSION     DASUNT010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884098"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VFX94_01015"
     CDS             complement(<1..263)
                     /locus_tag="VFX94_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766787.1"
                     /translation="MQRKLHPLEGIVAICALVLVLFVSVGFAQAQDVRKVALRSSSAL
                     VLDAATGEVVFGKNTDAVVPIASITKLMTAMVILDYGLDLDEEL"
     gene            complement(453..2378)
                     /locus_tag="VFX94_01020"
     CDS             complement(453..2378)
                     /locus_tag="VFX94_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEX5766788.1"
                     /translation="MAHSAAPPQRLRGNEWKALAFLLPYLWEYKGRVVLALACLVSAK
                     LANVGVPLVMKAVATLLPYLWEYKGRVFLALACLVTAKLANVGVPLVMKEVVDGLDPS
                     LQIVAVPVALLAVYGLLRFSTTLFQELRDVVFVRVTQRAIRRVALGVFRHLHSLSLRF
                     HLERQTGGMTRDIERGTRGISTLLSYLIFSIFPVILEFALVAAVLMAKFDWRFAAITF
                     GAVAAYIAFTVTVTEWRMDIRRRANELDSRANTRAVDSLLNYETVKYFGNEEFEARRY
                     DEQLHKYEDAATKNEASLGVLNIGQSLIIAIAVTALMLLVAQGVAAKAFTLGDLVLVN
                     GLLIQLYIPLNFLGMVYREVKQALLDMDRMFRLLSAHREVEDRPGAPELPRGPATVEF
                     KEVDFSYEKERQILFRVSFSIPAGQRVAVVGHSGSGKSTLARLLYRFYDVSAGAIRIN
                     GLDIREVRQSSLRAAIGIVPQDTVLFNDTILYNIRYGRPQAGDEEIYEAARAAHIHDF
                     IESLPRKYETQVGERGLKLSGGEKQRVAIARALLKNPRILIFDEATSALDSRAEKAIQ
                     AELERIAQGRTTLVIAHRLSTVMDADQILVLVQGRIVERGTHAQLLEAKGEYARMWSL
                     QQQQAEAQQILERTAAE"
     gene            2453..3325
                     /locus_tag="VFX94_01025"
     CDS             2453..3325
                     /locus_tag="VFX94_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5766789.1"
                     /translation="MGLRLKFNLVLLVVFVIGLGATGYVSYDLLHRNARDEVLRNAGV
                     MMEAALSMRGYTVAKVRPLLPYDADKFLPESVPAFAATEIMTLLRKTYPDFAYKEAAL
                     NPTNARNRAVEWEEDIIKAFRDEPARAEISGMRDTPTGRSLYLARPFQIKDPGCLACH
                     TTPEMAPPAMVKIYGSNNGFGWKENEVIGAQIVSVPMGLPMRNANRAFVTFMTSLAIV
                     FAALFVILNIMLTQLIIEPMTRLSDIAEQISKGKQDVPELVTQGDDEAAQLGQAFNRM
                     RRSLQKAISLIEKG"
     gene            3326..>3581
                     /locus_tag="VFX94_01030"
     CDS             3326..>3581
                     /locus_tag="VFX94_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX5766790.1"
                     /translation="MRPQVAANALPEGYALQEYRIQKVLGVGGFGLTYLALDSNLNLK
                     VALKEYLPADVAQRGTDQTIGPRSPSTAETFGWGKQRFMDE"
BASE COUNT          658 a         1164 c         1149 g          610 t
ORIGIN      
        1 agctcctcgt ccaggtcgag cccgtaatcg agaatcacca tcgcggtcat cagcttggtg
       61 atcgaggcga tcgggacgac cgcatcggta ttcttcccga agaccacctc gccggtcgcc
      121 gcgtcgagca ccagcgccga cgacgagcgc agcgcaacct tgcggacgtc ctgcgcctgg
      181 gcaaacccta cgcttacgaa gagcacgagc acgagggcgc agatcgcaac gatcccctcg
      241 agcggatgga gctttctttg catttgtgga tcttccccct cgacgcgcgc tgcctttttt
      301 gggtgtccat gcactgaacg gcgcatggac ccgtcgcaat atatcctacg tcgcaaaaaa
      361 aaagcaaacc aaaacagggc ttagcaattg ttttcaagaa agctcgtgag atctttcact
      421 gtcgggcgca ggcgtcgccc gtagcccttc ccctactcgg cggcagtgcg ttccaagatc
      481 tgctgggctt ctgcctgctg ctgctgcagg gaccacatcc tggcgtattc ccctttagcc
      541 tcaagcagtt gggcgtgggt tccgcgctcc acgatgcggc cctgaacgag caccaggatt
      601 tgatctgcgt ccatcacggt ggagagtcgg tgagcgatga caagcgtggt gcgtccctgc
      661 gctatgcgct cgagctcggc ctggatcgcc ttctcggcgc gcgaatcgag cgcggaagtg
      721 gcctcgtcga agatcaggat gcgcggattt ttcagcagtg cgcgcgcgat cgcgacgcgc
      781 tgcttctcgc ctccggagag cttgaggccg cgctcgccga cctgggtctc gtattttctt
      841 ggcagcgact cgatgaaatc gtggatatgc gcggcgcgcg ccgcctcgta gatctcctcg
      901 tcgcccgcct gcggccggcc gtagcgaatg ttgtagagga tggtgtcgtt aaaaagcacc
      961 gtgtcctgcg gcacgatgcc gatcgcggcg cgcaggctgc tttgccttac ctcgcggata
     1021 tcgaggccgt tgatccttat cgcgccagcg gacacgtcgt agaaccgata gagcaggcgg
     1081 gcgagcgtgg acttgcccga gcccgagtgg ccgaccaccg cgacgcgctg gccggcgggg
     1141 atcgagaagg agacgcggaa aaggatctgc cgttcctttt cgtaggagaa gtccacctcc
     1201 ttgaattcga ccgtcgcggg cccgcgcggc agctcgggcg cgcccggccg gtcctcgacc
     1261 tcgcggtgcg cggagagcag gcgaaacatc cggtccatgt cgagcagcgc ctgcttgacc
     1321 tcgcggtaca ccatgccgag gaaattgagc gggatgtaga gctggatgag caggccgttc
     1381 acgagcacga ggtcgccgag ggtgaaggcc ttcgccgcga cgccttgcgc cacgagcagc
     1441 atgagcgcgg tcaccgcgat cgcgatgatc aggctctgcc cgatgttgag cacgccgagc
     1501 gaggcctcgt tcttcgtcgc cgcgtcctcg tacttgtgca gctgctcgtc gtagcgccgt
     1561 gcctcgaatt cctcgttgcc gaagtacttc accgtctcgt aattgagcag gctgtcgacg
     1621 gcgcgggtgt tcgccctcga gtcgagctcg ttcgcgcggc gccggatgtc catgcgccac
     1681 tcggtgaccg tcacggtgaa cgcgatgtag gccgcgaccg cgccgaacgt gatcgccgcg
     1741 aagcgccagt cgaatttcgc catcagcacc gccgcgacca gcgcgaactc gagaatcacc
     1801 gggaaaatcg aaaagatgag gtatgaaaga agcgtcgaga tgccgcgcgt gccgcgctcg
     1861 atgtcgcgcg tcatgccgcc ggtctggcgc tcgaggtgaa agcgtagcga caggctgtgc
     1921 aggtggcgga acacgccgag cgcgacgcgg cggatcgcgc gctgcgtgac gcgcacgaac
     1981 accacgtcgc gcagctcctg gaacagcgtg gtcgagaagc gcagcagccc gtagaccgcg
     2041 agcagcgcca ccggtaccgc gacgatctgc aggctcgggt cgaggccgtc cacgacctct
     2101 ttcatgacca gcggcacacc gacgttggcg agcttcgcgg tcaccaggca ggcgagcgcg
     2161 aggaagaccc gccccttgta ttcccacagg tagggaagaa gggtagccac cgccttcatc
     2221 accagcggca cgccgacgtt ggcgagcttg gcgctcacca ggcaggcgag cgccagcacg
     2281 acccggccct tgtattccca cagataggga aggaggaacg caagcgcctt ccattcgttg
     2341 ccccgcaatc gctgcggggg agcggcgcta tgtgccatat gtccggatta tcgcaatccc
     2401 gcacatcagg ccgcccgcga cttacgtata gttgccgcca agggagatag gtatgggcct
     2461 caggctcaag ttcaacctcg tgctgctcgt cgtattcgtg atcgggctcg gcgcgaccgg
     2521 ctacgtgtcc tacgacctcc tgcaccgtaa cgcgcgcgac gaggtgctgc gcaatgccgg
     2581 cgtgatgatg gaagcggcgc tctcgatgcg cggctacacg gtggccaagg tgcggccgct
     2641 gctgccgtac gacgccgaca agttcctgcc cgaaagcgtg ccggccttcg ccgcgaccga
     2701 aatcatgacg ctgctgcgca agacgtaccc ggacttcgcc tacaaggagg cggcgctcaa
     2761 ccccacgaac gcgcgcaacc gtgcggtcga gtgggaggag gacatcatca aggcgttccg
     2821 cgacgagccg gcgcgcgcgg agatctcggg catgcgcgac actccgaccg ggcgctcgct
     2881 ctacctcgcg cgcccgttcc agatcaagga cccggggtgc ctcgcctgcc acaccacgcc
     2941 cgagatggcg ccgcccgcga tggtgaagat ctacggctcg aacaacggct tcggctggaa
     3001 ggagaacgag gtcatcggcg cgcagatcgt ctcggtgccg atggggcttc cgatgcgcaa
     3061 cgccaaccgc gccttcgtca ccttcatgac ctcgctcgcg atcgtgttcg ccgcgctgtt
     3121 cgtcatcctg aacatcatgc tgacgcagct catcatcgag ccgatgacgc ggctctccga
     3181 catcgcggag cagatctcca agggcaagca ggatgttccg gagctcgtca cccaggggga
     3241 cgacgaggcg gcgcagctcg gacaggcctt caaccggatg cggcgcagcc tgcaaaaggc
     3301 gatcagcctg atcgagaaag gatagatgag gcctcaggtc gccgcgaacg cactgccgga
     3361 aggctatgcc ctccaggagt accgcatcca gaaggtgctc ggcgtcggcg gcttcggcct
     3421 gacctacctt gcgctcgact cgaacctgaa cctcaaagtc gcgctcaagg agtatctgcc
     3481 ggccgacgtc gcgcagcgcg gcaccgacca gacgatcggg ccgcgctcgc cctccacggc
     3541 ggagaccttc ggctggggca agcagcgctt catggatgaa t
//
LOCUS       DASUNT010000053         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_445981, whole genome shotgun sequence.
ACCESSION   DASUNT010000053 DASUNT010000000
VERSION     DASUNT010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445981"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VFX94_01035"
     CDS             complement(<1..917)
                     /locus_tag="VFX94_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX5766791.1"
                     /translation="MQTETYNYKVVRQFAIMTVVWGVVGMLAGVFIAAQLLWPALNFD
                     VPWLSYGRLRPLHTNAVIFAFGGSALFATSYYVVQRTSHARLFGDGLAAFTFWGWQTV
                     IVLAAITLPLGFTSGKEYAELEWPIDLLIAAVWVAYAVVFFGTLWKRKVPHIYVANWF
                     FGAFILTVALLHIVNSAAVPAGFWKSYSAYAGAQDAMVQWWYGHNAVGFFLTAGFLGM
                     MYYFVPKQANRPIYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSVGMIFSLIL
                     LAPSWGGMINGIMTLSGAWHKLRDDPILK"
     gene            complement(977..1438)
                     /locus_tag="VFX94_01040"
     CDS             complement(977..1438)
                     /locus_tag="VFX94_01040"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEX5766792.1"
                     /translation="MPGFTRNWLAKSRRGARLPAAESAWTASLREPLLFVLHLGYVWL
                     ALGLVLVGLDASSAALHALTVGAIGTMTLAVMTRASLGHTGRALSADGATIAIYVLVT
                     LAALLRVLSPLAGAHAVTVNSLAGLAWSGAFGTFALHYGRFLIQGIPYRLT"
     gene            1602..>2346
                     /locus_tag="VFX94_01045"
     CDS             1602..>2346
                     /locus_tag="VFX94_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766793.1"
                     /translation="MTLYVSASRGLRSTQSWIAQQAEARLDALEQRIRAHLRPIGEQA
                     DWIADALADERIDFARPAELDAFMFGALGSTPHVSDLGIVDPFGRAWRWSRGARSSAR
                     ADWSQSAQVREWLVAGREQTGPAWRPLVWQEATALGPALLHQLPLHRGGRFVGMLGQV
                     VPMTKLSEDVAVFGAEYGVTAFVLYGPQADRVLAHRAFTGAPKMRERTGLPELAETED
                     PVLVRLHSPDGPALLSRALRGAKGVRASVG"
BASE COUNT          428 a          762 c          809 g          347 t
ORIGIN      
        1 aacttgagga tcgggtcgtc gcgcagctta tgccacgcgc ccgacagcgt catgatgccg
       61 ttgatcatcc cgccccagct cggggccagc aggatcagcg agaagatcat gccgaccgac
      121 tgcgtccagt ccggcagcgc ggtgtagtgc aggtggtgcg ggccggccca catgtaggtg
      181 aagatcagcg cccagaagtg caccaccgaa aggcggtagc tgtagatcgg gcggttcgcc
      241 tgcttgggca cgaagtagta catcatcccg aggaagcccg ccgtgaggaa gaagcccacg
      301 gcgttgtggc cgtaccacca ctgcaccatc gcatcctgcg cgccggcgta agcggagtac
      361 gatttccaga agcctgccgg caccgccgcg ctgttcacga tatggagaag cgcgacggtg
      421 aggatgaagg cgccgaagaa ccagttcgcg acgtagatgt gcgggacttt ccgcttccag
      481 agcgtcccga aaaacaccac ggcgtaggcg acccacaccg ccgcgatcag caggtcgatc
      541 ggccactcga gctccgcgta ttcctttccg gaggtgaatc ccagcggaag cgtgatcgcg
      601 gcaagcacga ttaccgtctg ccagccccag aacgtgaatg ccgcgaggcc gtcgccgaag
      661 agccgcgcat gcgaggtgcg ctgcaccacg tagtaggagg tcgcgaagag cgccgagccg
      721 ccgaaggcga agatcaccgc gttcgtgtgc agcggccgca ggcggccgta ggagagccac
      781 ggcacgtcga agttgagcgc aggccacagt agctgcgccg cgatgaaaac gcccgccagc
      841 atgccgacca cgccccagac cacggtcatg atcgcgaatt gccgcacgac cttgtagttg
      901 taagtctctg tctgcatggc actcctgccg ggaaacaggg gcccatgcta cgaagtcgtc
      961 cggagggccg tttgatttag gtcaagcggt agggaattcc ctgaatcaaa aagcgcccgt
     1021 aatgcagggc gaaagttcca aaggcgccgc tccaggcgag cccggcaagc gaattgacgg
     1081 tcactgcgtg cgcgccggca agcgggctca ggacccgcag caacgcggcg agggtcacga
     1141 ggacgtagat ggcgatcgtc gcgccatcgg cgctgagcgc acggccggtg tggccgagcg
     1201 aggcgcgcgt catcaccgca agcgtcatcg tgccgatcgc gccgactgtc agcgcgtgca
     1261 gggccgcgct cgaggcgtcc aggccgacaa gaaccagtcc gagcgcgagc caaacgtagc
     1321 caagatgcag gacgaaaagc agaggctcgc gcagcgatgc ggtccaggcc gactcggcgg
     1381 ccggcaggcg agcgccacgg cgagattttg ccagccagtt gcgggtgaag cccggcacga
     1441 tgcgccctac aaccagccca aacggaggcg agcagaaaga cggacggaag cccgccaaga
     1501 acaccttcct accatactat gatgccggtc gactgaagcg catttccttc gctaccgtcg
     1561 ccgtcacggg ccggggcgcg ttcgtcgcca tcgcggtcgg cgtgacgctt tacgtaagcg
     1621 cgtcgcgggg cctgcgctcg acgcagtcgt ggatcgcgca gcaggccgag gcccggctcg
     1681 acgcgctgga gcagcgcatc cgggcgcact tgcgcccgat cggcgagcag gccgactgga
     1741 tcgccgatgc actcgccgac gaacgcatcg atttcgcgcg gcccgccgag ctcgatgctt
     1801 tcatgttcgg cgcgctcggc tcgacgccgc acgtctccga ccttggcatc gtggatcctt
     1861 tcgggcgcgc ctggcgctgg agccgcggcg ctcgctcctc cgcgcgtgcc gactggtcgc
     1921 agtccgcgca ggtgcgggaa tggcttgtcg ccggccgcga gcagaccggc ccggcgtggc
     1981 gcccgcttgt ctggcaggag gcaaccgcgc tcggccccgc gcttttgcat cagctgccgc
     2041 tccatcgggg cggccgcttc gtaggcatgc tggggcaggt cgtgccgatg accaagcttt
     2101 cggaggatgt cgcggtgttc ggggcggagt acggggtgac cgccttcgtt ctgtatgggc
     2161 cgcaggcgga tcgcgtcctc gcgcaccgcg cattcaccgg cgcgccgaaa atgcgcgaac
     2221 gcactggctt gcccgagctc gcggagaccg aggatccggt gctcgtgcgg ctgcattcgc
     2281 ccgatgggcc cgcgctgctc tcgcgtgcgc taagaggggc gaaaggcgtg agagcgagcg
     2341 tcgggg
//
LOCUS       DASUNT010000054         2585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_182945, whole genome shotgun sequence.
ACCESSION   DASUNT010000054 DASUNT010000000
VERSION     DASUNT010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182945"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2585)
                     /locus_tag="VFX94_01050"
     CDS             complement(<1..>2585)
                     /locus_tag="VFX94_01050"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEX5766794.1"
                     /translation="GGEQSVTEVRRRVARALAQVEREGERVQWERRFLQAQEEGFIPA
                     GRINSAAGVALQATLINCFVQPVGDSISEMVDGRPGIYTALQEAAETMRRGGGVGYDF
                     SSIRPKGAEVKGTHSRASGPVSYMRVFDRSCETVESAGSRRGAQMGVLRCDHPDIEDF
                     IHAKDQGDLANFNISVGITDEFMAAVEKHLSFELVHKARPWQTNEEARQREDGMWVYR
                     SVRARDLWDQIMRSTYDHAEPGVVFLDRMNQDNNLYYCESIEATNPCSEQPLPPYGCC
                     CLGSIDLTKFVKEPFSDKSSFSFDHFGRVVAIAVRMLDNVLDVTAWPLEQQRDEAMAK
                     RRVGLGFTGLGDALIMLRLRYDSAEARVMAARISETMRDQAYRASAELAKERGAFPLF
                     NADLYLSGTSFATRLPESLKDLIRKQGLRNSHLLSIAPTGTISLAFADNASNGIEPPF
                     SWTYTRKKRVDGGWQEYSVEDHAFRRYRATGGNVESLPPYFVTALEMSARAHQEMVAA
                     VAPFIDSSISKTVNVPEDYPYSEFQDLYFLAWKSGLKGLATYRPNKVLGSVLSVQKTE
                     DFVQDDVNRRIEVKSVPAPVLASLRWPGRPDLSAGNPAWTYMIEHPQYRFAVFVGHVE
                     NGRPHAFEVWVNGSEQPRGLGALAKTLSMDMRANDSAWLKLKLDTLAKAGGDEPFEMP
                     FPPRGERKLMPSVVSAFAQIVRYRVEQLQSGLAQEVFQLAPEEASSPVLDAMFALKEP
                     KTGTDGTLSWTVDVLNPRTGDDFVLGLKEITLPDGITRPYSVWLSGEYPRALDGLCKI
                     LSLDMRVIDPAWIGMKLRKLLNYAEPLGHFMAFVPGLPGGQRRQQTWPSTVAYLARLV
                     IHRYA"
BASE COUNT          438 a          819 c          889 g          439 t
ORIGIN      
        1 tcgcgtagcg gtggatcacc aggcgcgcga ggtaggccac ggtggagggc caggtctgct
       61 ggcggcgctg gccaccggga agccccggca cgaaggccat gaagtggccc agcggctcgg
      121 cgtaattgag cagcttgcgc aatttcatgc cgatccaggc cgggtcgatc acccgcatgt
      181 cgagcgacag gatcttgcac agcccgtcga gggcgcgcgg gtattcgccc gagagccaca
      241 cggagtaggg gcgggtgatg ccgtccggca gggtgatttc cttcaagccc aggacgaagt
      301 cgtcgccggt gcgcggattg aggacatcca cggtccagga cagggtgccg tcggtgccgg
      361 tcttgggctc cttgagcgcg aacatcgcgt ccaaaacagg cgaggaagcc tcctcgggcg
      421 cgagctggaa aacctcttga gccaaacccg attggagctg ctccacccga tagcggacga
      481 tctgcgcgaa agcggagacg accgaaggca tgagcttcct ctcgccacgc ggcgggaagg
      541 gcatctcgaa gggctcgtcg ccgcccgctt tggcgagggt gtcgagcttg agcttgagcc
      601 acgcgctgtc gttcgcgcgc atgtccatgg agagggtctt cgcgagcgcg cccaggccgc
      661 gcggctgctc gctgccgttc acccagactt caaaggcatg cggcctgccg ttctcgacat
      721 ggccgacgaa caccgcgaag cggtactgcg ggtgctcgat catgtaggtc caggcggggt
      781 tgccggcgga gaggtcgggg cggccgggcc agcgcaggga agcgagcacc ggcgcgggaa
      841 ccgacttcac ctcgatgcgg cgattgacat cgtcctggac aaagtcttcc gttttctgaa
      901 ccgaaagaac agagccgagc accttgttgg gccggtacgt cgcgaggccc ttgagacccg
      961 atttccatgc gaggaagtac agatcctgga actcggagta cgggtagtcc tccggaacgt
     1021 tcaccgtctt cgagatgctg ctatcgatga acggggccac cgcggcgacc atttcctggt
     1081 gcgcccgggc gctcatctcg agcgcggtca cgaagtaggg cggcagcgat tccacattgc
     1141 cgcccgtcgc ccggtatcgc cggaaggcat gatcctcgac cgagtactcc tgccagccgc
     1201 cgtcgacgcg cttcttgcgc gtgtaggtcc acgagaacgg cggctcgatg ccgttggagg
     1261 cgttgtccgc gaacgcaagg ctgatcgtgc cggtgggcgc gatcgagagc aggtgcgagt
     1321 tgcgcaagcc ctgcttgcgg atcaggtcct tcagcgattc gggaaggcgc gtcgcgaagc
     1381 ttgtgccgga aaggtaaaga tcggcgttga agagagggaa ggcgccacgc tctttcgcaa
     1441 gctccgccga ggcgcgatag gcttggtcgc gcatggtttc ggagatgcgc gccgccatga
     1501 ctcgcgcttc ggcgctgtcg tatctcaggc gcagcatgat gagcgcgtcg ccgaggccgg
     1561 tgaagcccag gccgacgcgc cgcttcgcca tcgcctcgtc gcgctgctgc tcgagcggcc
     1621 aggcggtgac gtcgagcacg ttgtccagca tgcgaaccgc gatggcgacg acgcgtccga
     1681 aatgatcgaa gctgaaggaa gacttgtcgg aaaagggttc ctttacgaac ttcgtcaggt
     1741 cgatcgaacc caggcagcag cacccgtagg gcggcagcgg ctgctcggag cacggattgg
     1801 tcgcctcgat cgactcgcag taatagaggt tgttgtcctg gttcatgcgg tcgaggaaca
     1861 ccacgccggg ctctgcatgg tcgtaggtcg agcgcattat ctggtcccac agatcgcgcg
     1921 cgcgcacgct gcggtagacc cacatgccgt cttcacgctg gcgcgcttct tcgttggtct
     1981 gccagggccg cgccttgtgg acgagctcga aggaaaggtg cttctctacc gccgccatga
     2041 actcgtcggt gatgccgacc gagatgttga agttcgcgag gtcgccctgg tccttcgcgt
     2101 ggatgaagtc ctcgatgtcc gggtggtcgc agcgcagcac gcccatctgc gcgccgcggc
     2161 gcgagcccgc cgactccacg gtctcgcacg agcggtcgaa gacgcgcatg tatgacaccg
     2221 ggccgctcgc acgcgagtgc gtgcccttca cctccgcacc cttgggccgg atcgacgaga
     2281 aatcgtagcc gacgccgccg ccccggcgca tggtctcggc cgcttcctgc agcgcggtgt
     2341 agatgcccgg ccggccgtcc accatctccg agatcgagtc gccgacaggc tggacgaagc
     2401 agttgatgag cgtcgcctgg agcgctacgc cggcagcgga gttgatgcgg ccggcaggga
     2461 tgaagccctc ctcctgggcc tggaggaagc gccgctccca ttgcacgcgc tcgccttcgc
     2521 gctcgacctg cgcgagcgcg cgcgccacgc ggcgccgcac ttcggtgacc gactgctcgc
     2581 cgcct
//
LOCUS       DASUNT010000055         3819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1147478, whole genome shotgun sequence.
ACCESSION   DASUNT010000055 DASUNT010000000
VERSION     DASUNT010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147478"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VFX94_01055"
     CDS             complement(<1..690)
                     /locus_tag="VFX94_01055"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEX5766795.1"
                     /translation="MEAQSLKGKAAVVTGSTSGIGLGVAHALASQGADILLNGFGDRA
                     QIDKLVGDFKAQHRVKASYSAADMSKPADVAGMVAQATRELGRVDILVNNAGIQHTAP
                     IEEFPVEKWDAVIAINLSAAFHAIRAALPQMKSRNWGRIVNIASAHGLVASTQKVAYV
                     AAKHGLLGMTKVVALETATTGITCNAICPGWVLTPLVQKQIDERAANEKIPVGRAKME
                     LLSEKQPSLEFA"
     gene            746..1264
                     /locus_tag="VFX94_01060"
     CDS             746..1264
                     /locus_tag="VFX94_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5766796.1"
                     /translation="MKPNVYRASEILRDGRRLEVRALEPQDRDALVAAAGRSSAETLA
                     LRFFSPKRGFTEKEIEYYTKVDFVNHVALAAVVDEAGKPLIVGGGRYIVAQPGCAEVA
                     FTVEDAHQGRGIASLLLRHLVKIARAAGLKELFAEVLPDNAPMLKVFERSGIPMARKR
                     GRDAVHVTLRLD"
     gene            1306..1524
                     /locus_tag="VFX94_01065"
     CDS             1306..1524
                     /locus_tag="VFX94_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766797.1"
                     /translation="MKTLIALSSVLVAGCAADCGPDWRALGQRDGRLGAGSQAASYAA
                     RCNVTVDTAAYEQGYGEGFAMRPRVPYP"
     gene            1546..2247
                     /locus_tag="VFX94_01070"
     CDS             1546..2247
                     /locus_tag="VFX94_01070"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEX5766798.1"
                     /translation="MRRSLAIACFVPVAAIAADPGPFFDPGTYLGAGVRSHPEYDGAS
                     DQELMLIPLVRYYGPILFARTTQGILEGGARAELVKGLFAGVQLAYEEENDRTDLDPD
                     GSYGVHLEWDTRLGRMPVNLLARARFRFDSDNGSQTDLRASAGVYGTARLGVGVFGQA
                     TWGNSDWFRAYYATDGSGLVYTALGVEAQYELGRHWLLIGSAQARFLQGEASSSPITE
                     ERTNYYASAGLAYRF"
     gene            2259..3134
                     /locus_tag="VFX94_01075"
     CDS             2259..3134
                     /locus_tag="VFX94_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766799.1"
                     /translation="MRKVRVAPKGMGLNDFVAEEEGFFADEGIEVEWDIKTFRGTQSS
                     WKDLDYRSRPQDQPYAEGDKSLIQCACVWGSISNASAGMGRFVPDCYGVSPWAIFVRP
                     DSKIRRPQDLKDVPVSVGMRAGSHFNVPFRLEKYLPFENIKVVNTGGFGARLKALLDK
                     EVEAASLLPPQIDMARQLGLRMVMEDEFHTLWWVPETAPLDDVRAYLRALDKAEKALQ
                     ADLKKYLPLWEECIPPEFKNTHDWDYSKFTRGERFAFEPMPRAEFDETLAQVKRWKLD
                     QYLRETSFDRLTYRV"
     gene            3143..3292
                     /locus_tag="VFX94_01080"
     CDS             3143..3292
                     /locus_tag="VFX94_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766800.1"
                     /translation="MRFTRLTGFLLLLGLAACETTQSGYVYEGGSPMIDVPTPPFPYN
                     SKFCT"
     gene            3305..3571
                     /locus_tag="VFX94_01085"
     CDS             3305..3571
                     /locus_tag="VFX94_01085"
                     /inference="COORDINATES: protein motif:HMM:NF017742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HEX5766801.1"
                     /translation="MDAAGNKLMSELRAVVTAAEELLAATGSESAERLSELRGHAEEA
                     IRGARARLEGAGEELEDQVRQHPFAALGIAAAVGLVIGVLLARK"
     gene            3568..>3819
                     /locus_tag="VFX94_01090"
     CDS             3568..>3819
                     /locus_tag="VFX94_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX5766802.1"
                     /translation="MSTGLLASAQTFVASLLDLGRTRFELFSTELREELARLATALLG
                     GLAVLMLGALGLGFSGFALVVYVSEEKRLAAAIGVAVFFF"
BASE COUNT          639 a         1257 c         1270 g          653 t
ORIGIN      
        1 cgcgaactcg agcgacggct gcttctccga aagcagctcc atcttcgcgc gcccgacggg
       61 aatcttctcg ttcgccgcac gctcgtcgat ctgcttctgc acgagcggcg tcagcaccca
      121 gcccgggcag atcgcgttgc acgtgatccc ggtggtcgcg gtttcgagcg caaccacctt
      181 cgtcatcccc agcagcccat gcttcgccgc gacgtaggcc accttctgcg tcgaggcgac
      241 cagtccgtgc gccgaggcga tgttgacgat gcgaccccag ttgcgcgact tcatctgcgg
      301 cagcgcggcc cggatggcgt ggaaggccgc ggaaaggttg atcgcgatca ccgcatccca
      361 cttctccacc ggaaattcct cgatcggcgc ggtgtgctgg ataccggcgt tgttgacgag
      421 aatgtcgacc cggccgagct cgcgcgtcgc ctgcgccacc atgccggcga catcggccgg
      481 cttggacata tcggcagcgc tatagctcgc cttcaccctg tgctgcgcct tgaagtcccc
      541 gaccagcttg tcgatctgcg ccctgtcgcc gaagccgttg agaagaatgt cggcgccctg
      601 cgacgcgagg gcatgcgcca cgccgaggcc gatgccgctc gtcgagccgg tcacgacggc
      661 ggctttgccc ttgagggatt gagcttccat gtggcgatgc tagtaccatg atccgccccg
      721 caacaatcag ccttaggtat gtaaattgaa accgaacgtg tatcgcgcca gcgaaatatt
      781 gcgcgacggc cgccgcctgg aggtgcgcgc gctcgagccg caggatcgcg acgcgctggt
      841 cgccgctgcc ggccgctcga gcgccgagac gctcgcgctg cgcttcttct cgccgaagcg
      901 gggattcacc gagaaggaaa tcgagtacta caccaaagtg gacttcgtga atcacgtcgc
      961 gctggccgcg gtcgtagacg aggccggcaa gccgctgatt gtcggcggcg ggcgctacat
     1021 cgtggcgcag ccgggctgcg ccgaagtcgc ctttacggtg gaagatgccc accagggacg
     1081 cggcatcgcg tccctgctgc tgcgtcacct ggtgaagata gcgcgcgccg ccgggttgaa
     1141 ggagctcttc gcggaagtac tgccggataa tgcgccgatg ctcaaggtgt tcgagcgcag
     1201 cgggattccc atggcgcgca agcgcgggcg cgacgcggtg cacgtcacgc ttcggctgga
     1261 ctaaagtccc gaagagcggt gtttgccccc ctggaggcag acaccatgaa aacactcatt
     1321 gcgctgagca gcgtgcttgt tgccggctgc gccgccgatt gcgggcctga ctggcgcgcg
     1381 ctcggccagc gcgacgggcg tctcggtgcg gggtcgcagg cggcgagcta tgccgcgcgc
     1441 tgcaatgtca cggttgatac cgcggcgtac gagcaaggct acggcgaagg cttcgccatg
     1501 cggccgcgcg tgccctaccc ttagagactg tgctagaaag cgtccatgcg gcgctcactg
     1561 gcgatcgcct gctttgtccc tgtcgcggcg atcgccgcgg accccggccc gttcttcgac
     1621 cccggcactt acctcggcgc gggcgtgcgc tcgcaccccg agtacgacgg cgcctccgac
     1681 caggagctca tgctcatacc gctcgtgcgc tattacggcc cgattctttt cgcgcgcact
     1741 acgcagggaa tcctggaagg cggcgcgcga gccgagctcg tcaaagggct cttcgcgggc
     1801 gtgcagctcg cttacgagga ggaaaacgac cgcaccgacc tcgaccccga cggctcgtac
     1861 ggcgtgcacc tcgaatggga cacgcgcctc gggcgaatgc cggtcaacct gcttgcgcgc
     1921 gcgcgctttc gattcgactc ggataacggc agccagacgg acctgcgcgc gagcgcgggt
     1981 gtctacggca ccgcgcggct cggcgtcggc gtcttcggcc aggccacctg ggggaacagc
     2041 gactggttcc gcgcctacta tgccaccgac ggcagcgggc tcgtttacac ggcgctcggc
     2101 gtcgaagccc agtacgagct gggccggcac tggctcctga tcggaagcgc gcaagcccgc
     2161 tttctccagg gcgaggcttc gtcgagcccg attaccgaag agcggacgaa ctactacgcg
     2221 agcgcgggct tagcatatcg cttttgaaag gaccggccat gcgcaaagtt cgagtcgcgc
     2281 ccaagggcat ggggctcaac gacttcgtcg ccgaggagga aggcttcttc gccgacgaag
     2341 gcatcgaggt cgaatgggac atcaagacct tccgtggcac gcagtcgagc tggaaggacc
     2401 tcgattacag gtcgcgcccg caggaccagc cctacgccga gggcgacaag agcctgatcc
     2461 agtgcgcctg cgtgtggggc agcatctcca acgcctccgc cggcatggga cgcttcgtcc
     2521 cggactgcta cggcgtctcg ccgtgggcga ttttcgttcg gccggattcg aagatccgcc
     2581 gcccccagga cctgaaggac gtccccgtat cggtcgggat gcgcgccggc agccacttca
     2641 acgttccctt ccggctcgaa aaatacctgc cgttcgagaa catcaaggtg gtgaacaccg
     2701 gcggctttgg cgcgcgcctg aaagcgctgc tcgacaagga agtcgaggcg gcgagcctgc
     2761 ttccgccgca gatcgacatg gcgcgccagc tcggcctgcg catggtgatg gaggacgagt
     2821 tccacacgct gtggtgggtt cccgagaccg cgccgctcga cgatgtgcgc gcgtacctgc
     2881 gcgctctcga caaagcagag aaagcgctgc aggccgacct gaaaaaatat ctgcccctgt
     2941 gggaagagtg tattccgccg gagttcaaga acacgcatga ctgggactat tcgaaattca
     3001 cccgcggcga gcgcttcgcg ttcgagccga tgccgcgtgc cgagttcgac gagacgctgg
     3061 cccaggtcaa gcgctggaag ctcgaccagt atctcaggga gacatccttc gacaggctca
     3121 cgtaccgggt ataggaggct gcatgcgctt cacccgcctg actggatttt tgctgttgct
     3181 cggcctcgcc gcgtgcgaaa caacgcaatc cggatacgta tacgaaggcg gctcgccgat
     3241 gatcgatgtg ccgacaccgc ctttcccgta caactcgaag ttctgcactt gaagggagag
     3301 catcatggat gcagccggta acaagctgat gtcggaattg cgcgccgtgg tgaccgcggc
     3361 ggaggagctg ctcgccgcga cgggcagcga gagcgcggag cggctgagcg agctgcgcgg
     3421 ccacgccgag gaggcgatcc gcggcgcgcg cgcgcgcctc gagggcgcgg gcgaagagct
     3481 cgaggaccag gtgcgccagc atccgttcgc ggccctcggc atcgccgctg cggtcggact
     3541 ggtgatcggc gtgctgctcg cgcgcaaatg agcaccggcc tgctcgcgtc cgcgcagacc
     3601 ttcgtcgcga gcctgctcga cctcggccgc acgcgcttcg agctcttcag caccgagctg
     3661 cgcgaggagc tcgcgcggct tgccaccgcg ctgctcggcg ggctcgcggt gctgatgctc
     3721 ggcgcgctcg gcctggggtt ctcgggattc gctcttgtgg tttacgtgag cgaggaaaag
     3781 cgcctggctg cggcgatcgg cgtcgcggtg ttcttcttc
//
LOCUS       DASUNT010000056         2589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_183130, whole genome shotgun sequence.
ACCESSION   DASUNT010000056 DASUNT010000000
VERSION     DASUNT010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183130"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFX94_01095"
     CDS             complement(<1..189)
                     /locus_tag="VFX94_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX5766803.1"
                     /translation="MHVLYEENGELKAGTVLERSPASLHVESPHGRRSKIKAASVLLS
                     FERPAPAELLREAESFSRG"
     gene            complement(173..736)
                     /locus_tag="VFX94_01100"
     CDS             complement(173..736)
                     /locus_tag="VFX94_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HEX5766804.1"
                     /translation="MIVYLHGFNSSPQSHKAQVLQRYMAEHGLAHEYACPALPPRARD
                     AVRAIEKPSLAPGVCFVGSSLGGFYATYLAEKHALRAVLVNPAIDPHVGLRAFLGPQK
                     NLHTGEPYELTEAHLRDWEALYVPRITPSRYLLIVETGDEVLDYRRAVERYAGAEQVV
                     IAGGDHSLQSFPQHLARILQFSRARSL"
     gene            complement(711..2069)
                     /gene="mpl"
                     /locus_tag="VFX94_01105"
     CDS             complement(711..2069)
                     /gene="mpl"
                     /locus_tag="VFX94_01105"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011205248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEX5766805.1"
                     /translation="MAAHLHILGICGTFMGGIAAIAKSAGYRVTGCDANVYPPMSDQL
                     RALGIELTEGFGAEQLKLRPDVWVIGNVVTRGNPLMEAVLDAGERYVSGPQWFADAVL
                     AGKHVLAIAGTHGKTTVSAMLAWVLERCGRAPGFLIGGIPNDFPVSARLTPTPLFVIE
                     ADEYDTAFFDKRSKFVHYRPRTAVLNNIEFDHADIFDDVAAIERQFHHFVRIMPRSGR
                     LVANGTDESVKRVLTLGCWTPVEAFGVPDGWEASLAQSDGSFEVAFAGRVLGRVRWAL
                     LGAHNRMNALATLAAARHVGVEPGEAIEALGSFGGVRRRMQVRGEARGVTVYDDFAHH
                     PTAIRTTLEGLRQRVGTARILAVLEPRSNTMRRGIMKDLLPQSVAPADRVYVYTSGLG
                     WDARSLFALLGSRVRCEDDLDSLIGALAAEAKAGDHVLIMSNGAFGAIHEKLLDRLSA
                     RL"
     gene            2090..>2589
                     /locus_tag="VFX94_01110"
     CDS             2090..>2589
                     /locus_tag="VFX94_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766806.1"
                     /translation="MNSTVGRKGTRQSVTRARSQAMRARISAAAARIMAEDGIDDFAL
                     AKRKAARQLGASETEALPANDEVETELRAYRALYQPQEHSRRIAELRRVALDAMRALE
                     RFSPYLTGAVLKGTAGRYAEIELQLFPESAKEVEIFLLERRIPYATREGRRFSGDRAH
                     AMSVLTL"
BASE COUNT          466 a          844 c          849 g          430 t
ORIGIN      
        1 tccgcgggaa aagctttccg cctcgcggag cagctcggcg ggcgccggcc gctcgaacga
       61 aagcagcacg ctggcggcct taatcttcga gcggcggccg tgcgggctct cgacatggag
      121 cgacgccggc gagcgctcga gcacggtgcc agccttcagc tcgccgtttt cttcatagag
      181 aacgtgcacg ggaaaactgc agtatgcgcg ccagatgttg cggaaagctc tgcaggctgt
      241 ggtctccgcc ggcgatgacc acctgctccg cgcccgcgta gcgctcgacg gcgcgccggt
      301 agtcgagcac ttcgtcgccg gtttcgacga tcaggagata gcggctcggc gttatgcgcg
      361 ggacgtacag cgcttcccag tcgcgaagat gggcctcggt aagctcgtag ggctcgccgg
      421 tgtgcaggtt cttctgcggc ccgaggaagg cgcgcaggcc gacgtgcgga tcgatcgccg
      481 gattgacgag caccgctcgc agggcgtgct tctcggcgag gtaagtcgcg taaaagcctc
      541 ccaacgaaga gcccacgaag cacacccccg gcgcgaggga cggcttttcg atcgcgcgca
      601 cggcatcgcg cgcgcgcggc ggcagggcgg gacaggcgta ttcgtgagca aggccgtgct
      661 cggccatgta gcgctgcagg acctgcgcct tgtgagactg gggcgaggag ttaaagccgt
      721 gcagatagac gatcaagcag tttctcgtga atggcgccga aggcgccgtt cgacatgatc
      781 agcacgtggt cgcccgcctt cgcctcggcc gcaagcgccc cgatcagcga gtcgagatcg
      841 tcctcgcagc gcacacgcga cccgagcagc gcgaaaagcg agcgcgcgtc ccagccgagg
      901 cctgacgtgt agacatacac gcgatcggcc ggggccacgc tttgcggcag caggtccttc
      961 attatccccc gcctcatggt gttcgagcgc ggctcgagta ccgcgaggat gcgtgccgtt
     1021 ccaacccgtt gccgaagccc ctcgagcgtg gtgcgtatcg ccgtggggtg atgcgcgaaa
     1081 tcgtcgtaaa cggtgacccc gcgcgcctct ccgcgtacct gcatgcggcg gcgcaccccg
     1141 ccgaagctgc caagcgcctc gatcgcctct ccgggctcaa cgccgacatg gcgcgccgcc
     1201 gccagcgtcg caagggcgtt catccggtta tgcgctccaa ggagcgccca gcgcacccga
     1261 ccaaggaccc gcccggcaaa agcgacttcg aagctcccgt cggattgggc caggctcgcc
     1321 tcccaaccat ccggtacgcc gaaggcctcg accggcgtcc agcatcctaa cgtgaggact
     1381 cgctttacac tctcgtccgt gccgttggct accagtcgtc ccgatctagg cattatcctt
     1441 acgaaatgat gaaattgccg ctcaatcgct gcaacatcat cgaagatgtc agcatgatcg
     1501 aattcaatgt tgttgagaac ggccgttcgc ggccggtaat ggacgaactt cgaccgtttg
     1561 tcgaaaaacg cggtgtcgta ttcgtccgcc tcgattacga agagcggggt gggggtgaga
     1621 cgcgcggaaa ccggaaagtc gttcggtatg ccgccaatca ggaatccggg tgcacggccg
     1681 caccgctcca ggacccaggc aagcattgcc gagacggtcg tcttgccatg cgttccggcg
     1741 atagcgagca cgtgctttcc tgccagtact gcatcagcga accactgcgg ccccgacaca
     1801 tagcgctcgc cggcatcgag gacagcctcc atcagcggat tgccccgggt gacgacgttg
     1861 ccgattaccc agacatcggg ccgcagcttc agctgctcgg cgccgaatcc ctccgtcagc
     1921 tcgatgccga gcgcgcgcag ctggtcgctc atcgggggat atacgttggc gtcgcagccc
     1981 gtcacgcggt agccggcgct cttggcgatg gcggcgatcc cgcccatgaa cgtgccgcag
     2041 ataccgagga tatggagatg cgctgccatg aagcgacgag attctatgat tgaattccac
     2101 ggtgggccgc aaaggcactc gccagagcgt aacgcgcgcg cgcagccagg cgatgcgtgc
     2161 gcgtatctcg gcagcagcag cgcgcatcat ggcggaggac ggtatagacg acttcgcgct
     2221 cgcgaagcgc aaggcggcgc ggcagctcgg cgctagcgaa accgaggcgc tgcctgcgaa
     2281 cgacgaggtc gagaccgagc ttcgcgccta tcgcgcgctt tatcagccgc aggagcactc
     2341 gcggcgcatc gcggagctgc gccgcgtcgc gctcgacgcc atgcgtgcgc tcgagcggtt
     2401 cagtccgtat ctcaccgggg cggtgctgaa gggcaccgca gggcgctacg cggaaatcga
     2461 gctgcagctt tttcccgaaa gtgcgaagga ggtcgagatc tttctgctcg agcggcgcat
     2521 tccgtacgcc actcgcgaag gtcggcgctt ctcgggcgat cgtgcccatg cgatgagcgt
     2581 cctgacgct
//
LOCUS       DASUNT010000057         3212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_358718, whole genome shotgun sequence.
ACCESSION   DASUNT010000057 DASUNT010000000
VERSION     DASUNT010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358718"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1721)
                     /locus_tag="VFX94_01115"
     CDS             complement(<1..1721)
                     /locus_tag="VFX94_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5766807.1"
                     /translation="MSLRLEHLNPEQLAAVTLPDEHALILAGAGSGKTRVLTTRIAWL
                     IKGGRVAPSGILAVTFTNKAAREMLTRVAAMMTGNPRGMWIGTFHGLCNRMLRAHARE
                     AGLAPAFQILDSQDQLGAIKRLLKAMNVDEERFPAREVQYFINDRKEEGVRARDVAPA
                     DENTRRFTELYAAYDEQCQREGVVDFPELLVRSYELLSRNEILREHYARRFRHILVDE
                     FQDTNRLQYRWLKLLRSQDAKYFCVGDDDQSIYTFRGAYVGNMADFEREFKVAQVIRL
                     EQNYRSHGNILDAANALIAINRNRLGKNLWTAAGKGEPVRVFEGESDADEARWIVEEV
                     QALAREGTALSQMAVLYRSNAQSRVVEHALFSQGIAYRVYGGLRFFERAEIKHALAYL
                     RLIATPEDDNAFLRVANFPPRGIGARTIEQLQDAAKQAGVSLYKASEGNAKAGPFRLL
                     IENLRKQTEGLPLKEVVDAVIARSGLIEHYKTEREGADRIENLEELVNAAAAFTEEER
                     VSESGEALDPLTAFLTHAALEAGEHQAGEGQDALQMMTVHSAKGLEFDVVFISGLEEG
                     LFPHEQSV"
     gene            complement(1731..2684)
                     /locus_tag="VFX94_01120"
     CDS             complement(1731..2684)
                     /locus_tag="VFX94_01120"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5766808.1"
                     /translation="MKKVFLLAVLAFAAGAQAQTWPSKTVRFIVPFPPGGSTDVAART
                     LADKLTRALGAQVLVENRAGGGGAIGTVEAARAAPDGHTLLFAADPVITLHLVVKNVQ
                     FDMQRDFIAVTQVTTQPIAVAIHSSLPVKDVQELIAYAKANPGKLSFAHSGTGSGQHM
                     SGELLKKMAGIDMVHVPYKGGGPAVQDLVGGQVPVGVLGSTPLIPHHKAGRIRIIAFT
                     SKERFPPMPEIPTLHESGLAGFDTTQWLGILAPKGTPADIVDRVYAETRKALELQDVK
                     ERLAQAALQPVGNSPKEFEALIRADLERWTAVAKELKLEPQ"
     gene            complement(2681..>3212)
                     /locus_tag="VFX94_01125"
     CDS             complement(2681..>3212)
                     /locus_tag="VFX94_01125"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX5766809.1"
                     /translation="VASKLRKREVSVRVDTVTIGVRGTDFWGRSRGELQIVCLIEGAI
                     QVGAPGEQAVTMDQPRQFYRRDKGATQPVGLVEAKQLEQWALETDIQAGKGALRRDGR
                     FSIQLLTAADQARANSVSNRLREAGYPAEIASSKEAEKATHMVRIRYLPSRDEAQALA
                     NRLKGSFGISDPRVMP"
BASE COUNT          493 a         1085 c         1040 g          594 t
ORIGIN      
        1 acgctctgct cgtggggaaa gaggccctcc tcgagcccgg agatgaacac cacgtcgaac
       61 tccaggccct tggccgagtg cacggtcatc atctgcagcg cgtcctgccc ctcgcccgcc
      121 tggtgctcgc ccgcctcgag cgccgcgtgg gtcagaaacg cggtgagcgg gtcgagcgcc
      181 tcgccggact cgcttacccg ctcctcctcg gtgaacgcag ccgccgcgtt caccagctcc
      241 tccaggttct cgatccggtc cgcgccctca cgctcggtct tgtagtgctc gatgagcccg
      301 cttctagcga tcaccgcatc gaccacttcc ttgaggggca gtccttcggt ttgctttctc
      361 aggttttcaa taagaagacg gaaagggccc gccttggcgt tgccttccga ggctttgtac
      421 agtgacaccc ctgcctgctt ggccgcgtcc tgcagctgtt cgatggtgcg cgcgccgatg
      481 ccgcgcggcg ggaaattggc gacccgcaga aaagcgttgt cgtcctccgg ggtggcgatg
      541 aggcgcagat atgcgagtgc gtgcttgatc tccgctcgct cgaagaagcg caagcccccg
      601 tacacgcggt acgcaatgcc ctgcgagaag agcgcgtgct ccacgacccg cgactgggcg
      661 ttcgagcggt aaagcaccgc catctgcgaa agcgcagtgc cctcgcgcgc gagtgcctgc
      721 acctcctcca cgatccagcg cgcctcgtcg gcgtccgact cgccctcgaa cacccgcacc
      781 ggctcgccct tgccggcagc ggtccacaga ttcttgccca agcggttgcg gttgatcgcg
      841 atgagcgcgt tggccgcgtc caggatgttg ccgtgcgagc ggtagttctg ctcgaggcga
      901 atcacctgcg cgaccttgaa ctcgcgctcg aagtcggcca tgttgccgac atacgcgccg
      961 cggaaggtgt agatgctctg gtcgtcgtca ccgacgcaga aatatttcgc gtcctgcgaa
     1021 cgcaagagct tcagccatcg gtactgcagg cggttcgtgt cctggaactc atcgaccagg
     1081 atgtgccgga agcgcctggc gtagtgctct cgcaggatct cgtttctcga cagcagctcg
     1141 tacgagcgta cgagcagttc aggaaaatcc accacgccct cgcgctgaca ctgctcgtcg
     1201 taggcggcgt agagctcggt gaagcggcgc gtgttctcgt cggccggcgc aacgtcgcgc
     1261 gcgcgcacgc cttcttcctt gcgatcgttg atgaagtact gcacctcgcg cgcggggaag
     1321 cgctcctcgt cgacgttcat cgctttcagc agtcgcttga tggcgccgag ctggtcctgc
     1381 gagtccagga tctggaacgc cggcgccagc ccggcctcgc gcgcatgcgc acgcagcatg
     1441 cggttgcaca ggccgtggaa ggtgccgatc cacatgccgc gcggattgcc cgtcatcatc
     1501 gcggcgacgc gggtcagcat ctcgcgcgcc gccttgttgg tgaaggtcac cgcgaggatt
     1561 cccgacggcg cgacacgccc gcctttgata agccaggcga tgcgtgtagt gagcacccgc
     1621 gtcttgccgc tacccgcgcc ggcgaggatc agcgcgtgct catccggcag ggtgaccgcg
     1681 gcaagctgct cggggttgag gtgttcgagg cgaagcgaca agccgccatt ttactgaggc
     1741 tcgagcttca attccttggc aactgcggtc cagcgctcca gatccgcgcg gatcagcgcc
     1801 tcaaattctt tcggcgagtt gccgaccggc tgcagcgcgg cttgcgccag cctttccttc
     1861 acatcctgga gctcgagtgc cttgcgggtc tctgcgtaaa cccggtccac gatgtcggcc
     1921 ggcgtgccct tcggcgcgag gatgccgagc cactgcgtgg tgtcgaagcc tgcgaggccc
     1981 gactcgtgca gcgtcgggat ctcgggcatc ggcgggaagc gctccttgga ggtgaaggcg
     2041 atgatgcgga tgcggcccgc cttgtggtgc gggatgagcg gcgtcgagcc cagcacaccg
     2101 actggcacct ggccgccgac gagatcctgg accgcgggcc caccgccctt atacggcacg
     2161 tgcaccatgt cgatcccggc catcttctta aggagctcgc ctgacatgtg ctggccgctg
     2221 ccggtgcccg agtgcgcgaa cgatagcttg ccggggttcg ctttggcgta ggcgatgagc
     2281 tcctggacat ccttcaccgg cagcgagcta tgtatggcca ccgctatcgg ctgcgtggtc
     2341 acctgcgtca ccgcgatgaa gtcgcgctgc atgtcgaact gcacgttctt cacgacaagg
     2401 tgcaacgtaa tgaccggatc ggcggcgaaa agcagcgtgt ggccgtcggg tgcggcgcgc
     2461 gccgcttcca ccgtgccgat ggcccctccg ccgccggcgc ggttctcgac caagacctgc
     2521 gctccgagcg cgcgggtcag cttgtcggcg agcgtgcgcg cggcaacgtc ggtcgagccg
     2581 ccgggcggga aaggcacaat gaagcgcacg gtcttcgacg gccaggtttg cgcctgggcg
     2641 cccgctgcga aagcaaggac tgcaagaaga aagaccttct tcatggcatg actctcgggt
     2701 cgctgatacc gaagctgccc ttcaggcggt tggcgagcgc ctgcgcttcg tcgcgcgaag
     2761 gcaggtaacg gatgcgaacc atgtgggtgg ctttctcggc ttctttgctg ctggcgatct
     2821 ccgcagggta acccgcttcg cgcagccggt tgctgacgct gttcgcccgc gcctggtccg
     2881 cggcggtcag cagctggatg ctgaagcgcc cgtctcgacg cagcgcgccc ttgcccgcct
     2941 ggatgtcggt ctcgagcgcc cactgctcga gctgcttggc ctcgaccagg ccgaccggct
     3001 gggtggctcc cttgtcccgt cggtagaact gccgcggctg gtccatcgtc acggcctgct
     3061 ccccgggagc gccgacctga atcgcacctt cgataaggca gacgatctgc agctctccgc
     3121 gcgagcggcc ccagaagtcc gtaccgcgga cgccgatcgt caccgtatcg acgcgcacgc
     3181 tgacctcgcg cttgcgcagc ttcgacgcca cg
//
LOCUS       DASUNT010000058         4644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_183306, whole genome shotgun sequence.
ACCESSION   DASUNT010000058 DASUNT010000000
VERSION     DASUNT010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183306"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VFX94_01130"
     CDS             complement(<1..639)
                     /locus_tag="VFX94_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766810.1"
                     /translation="MAAASTGASRRTVAATVGTPEATLRRWINRGEAYPDEEPYGSFA
                     RDYKQAERGLAASAAGTVAMKAQLLNEQMVKRLEWEKRKPLGAKPAKPKKPGKGASLQ
                     ERIDYARALEAYPAELADWEARKEADSVPPPTPDVGEMLWLEKVRISRHPEDYGTSKH
                     RKPDTPFDGLEYLNETAMDRAQLAALFSDPPEEMRLALQDAARHVYAILVEGG"
     gene            complement(856..1200)
                     /locus_tag="VFX94_01135"
     CDS             complement(856..1200)
                     /locus_tag="VFX94_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766811.1"
                     /translation="MFTDLTAKELAFRELIAGVEVRPGRVEINPTYSLECAAARLRWE
                     RANIVWFHGTNEKGDPVWSRERPGPELAARLMSHNEHVYMRRVDFLAAWAEAKETGKP
                     QPRFVLVRSISG"
     gene            complement(1269..1568)
                     /locus_tag="VFX94_01140"
     CDS             complement(1269..1568)
                     /locus_tag="VFX94_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766812.1"
                     /translation="MTTSRGNAHWVRAMIRNQYVDQFVALGSLYAAGYEANEEEAIRE
                     ELAELGIPVGGRMHSAPRQLEPHELVARDDGAPEEELANIERDIPSAARWHGWHW"
     gene            complement(1568..2575)
                     /locus_tag="VFX94_01145"
     CDS             complement(1568..2575)
                     /locus_tag="VFX94_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766813.1"
                     /translation="METLNRYNVHCFGAEPDADYDIERTPEGRWMEAADVLRVLGERD
                     AVEQDRSEAYERVCEERRILECKLDESILRERSKQRRCEVLVAMDAELIEQRDRAFTS
                     ARLANKRRDVDRAEILQLRQRVGHLTGRVIDSAVLLNRLVNGGTKNPWEVVQQAREFV
                     KLLRKDTEGYTMTGPCDIPLLYGKIDLLKEDGTPMPTNEELEKKASKSDTGIHSCRNC
                     EDGGHPDFCSLPAPEPGVITRPATDGTPVAVHPEFAKVYDEAPGLWQGTIDATAADAQ
                     RLGIDPAKPSEDDKRALMQAVVDAPPALTDHDACRDPNCPGHQAVEGDGDTLPEPDIQ
                     F"
     gene            complement(2563..2787)
                     /locus_tag="VFX94_01150"
     CDS             complement(2563..2787)
                     /locus_tag="VFX94_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766814.1"
                     /translation="MSLGQGKALRRSSTGLALLIQLEGESRWIPISQLHDDSEVYQPG
                     HEGNVIVLEWWADKEGLSDVKTKGKDPWKR"
     gene            complement(2796..3620)
                     /locus_tag="VFX94_01155"
     CDS             complement(2796..3620)
                     /locus_tag="VFX94_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766815.1"
                     /translation="MNAQTEASVHALMREATRLGFSVCPNEQWKQHVEQLDRTTRERE
                     QLTERLLQQSDLVHELEVRAERWGKAFDVAVELRENIEHSMLEKIEELTDRISELTLM
                     HDDAVKGERESDDRLEKATRLVFRCLDRLGVKFETHINIETALAELYRRLMADPSVEP
                     IPAGELPFEAKSGAALLLQYIRRNGLGVMLDEETLTAWWANIAMAGYDQHARRYSKLI
                     ELAIMGERINGGSCYSQEAENAIAEEANRFVDPGAPPTGWSPPPAPSTEPAPQVEL"
     gene            complement(3617..3907)
                     /locus_tag="VFX94_01160"
     CDS             complement(3617..3907)
                     /locus_tag="VFX94_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766816.1"
                     /translation="MYVVSREDQQQRCDNRHPDWVQHGGQAPNICKQAPEGDLELEEL
                     AVRVELDPTLTELITAVRRAAGELEGQGAKSDRIAQQLFAALATFHLKTGRL"
     gene            complement(3977..4222)
                     /locus_tag="VFX94_01165"
     CDS             complement(3977..4222)
                     /locus_tag="VFX94_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766817.1"
                     /translation="MAEATRRERDVLVALLEYPHEHEGKHPSLSEIASVLELTKAGAQ
                     RHVSALQLKGKAELRPGGGWSVTKSGRAELKRHGLIT"
BASE COUNT          636 a         1593 c         1502 g          913 t
ORIGIN      
        1 gccgccctcc acgaggatgg cgtacacgtg ccgcgcggcg tcctgcagcg cgagccgcat
       61 ctcctccgga gggtccgaga acagcgccgc gagctgcgcg cgatccatgg ccgtctcgtt
      121 gaggtattcg aggccatcga acggcgtgtc gggcttgcga tgcttgctcg tgccgtagtc
      181 ctcggggtgc cggctgatcc gcaccttctc gagccagagc atctcgccca cgtcgggtgt
      241 cggcgggggc acgctgtcgg cttctttgcg cgcctcccag tcggcgagct cggcggggta
      301 ggcctcgagc gcgcgggcat agtcgatgcg ctcctgcagg ctggcgccct tgcctggctt
      361 cttcggcttc gccggcttgg cgccgagcgg cttgcgcttc tcccattcga ggcgcttcac
      421 catctgctcg ttgagcagct gcgctttcat ggcgaccgtg cccgcagcgc tcgccgcgag
      481 cccgcgctct gcctgcttgt agtcacgggc gaaggagccg tagggctcct cgtcggggta
      541 ggcctcgcct cggttgatcc agcgccggag cgtcgcctcg ggtgtgccta cagtggcggc
      601 aactgtgcga cgactggcgc cggtggaagc agcggccacg caacgcgttc ggtgcgccgg
      661 gtcctctatt ccactgtagg gccctgttcg ccgcctacgt tgtaactggt atctcattcg
      721 gggtgcgcca tcagtttgcg ccacattcgg ccccgttgcc atccggcgag ccgatcccgg
      781 agactcgaca ccagcgcgcc ttcacgacgc cgagccgctg cggggaggcg gggaggctca
      841 cctctgggga cgccttcagc cgctgatgct gcgcacgaga acgaagcgcg gctgaggctt
      901 gcccgtctct ttcgcttcgg cccatgcagc caggaagtcc acgcgccgca tgtagacgtg
      961 ctcattgtgg ctcatcagcc gcgcagcgag ttcgggcccg gggcgctcgc gagaccagac
     1021 gggatcgccc ttctcgttcg tgccgtgaaa ccacacgatg tttgcccgct cccagcgcag
     1081 ccgcgcagcg gcgcactcga gcgagtaggt gggattgatc tcgacccgcc cagggcgcac
     1141 ctcgacaccc gcgatcagct cccgaaacgc gagctccttc gctgtaaggt ccgtgaacac
     1201 gggcccttca gggcgctgca gcttgcgcga aggttttttt tcgacaggcc cgacggggcg
     1261 ccggctcgtc accagtgcca cccgtgccac cgtgcagccg acggaatatc gcgctcgatg
     1321 ttcgcgagct cctcctccgg agcaccatcg tcgcgcgcta cgagctcgtg cggctccagc
     1381 tgccggggag cgctgtgcat gcgccccccg acagggatac cgagctcggc gagctcctcg
     1441 cggatcgcct cctcctcgtt cgcttcgtaa ccagcggcat agagtgagcc gagggcgacg
     1501 aactggtcca cgtattggtt tcggatcatc gcacgcaccc agtgcgcgtt gcctcggctc
     1561 gtggtcatca gaactggata tctggttcgg gtagcgtgtc gccgtcgccc tcgacagcct
     1621 gatggcctgg gcagttgggg tctctgcaag cgtcgtggtc ggtcagcgcc ggcggagcat
     1681 caacgacagc ctgcatcagc gcgcgcttgt cgtcttcgct cggcttggcg gggtcgatgc
     1741 ccaggcgctg cgcgtccgcc gcggtggcgt cgatggtgcc ctgccagagc cccggcgcct
     1801 cgtcgtagac cttggcgaac tcggggtgca ccgcgacggg cgttccgtcg gtggcggggc
     1861 gagtgatgac accaggctcg ggcgcaggga gagagcaaaa gtcggggtgt ccgccatcct
     1921 cgcagttccg acacgagtgg attccagtgt cggatttcga ggccttcttt tcgagctcct
     1981 cgttcgtggg catcggcgtt ccgtcctcct tcagcaggtc gatcttgccg tagagaagcg
     2041 ggatgtcgca cggcccggtc atcgtgtagc cctcggtgtc tttgcgcagg agcttgacga
     2101 actcgcgcgc ctgctgaacc acctcccaag ggttcttggt gcctccgttc accagccgat
     2161 tcagcagcac ggcggagtcg atgacccgtc ccgtcaggtg cccgacgcgc tggcgcagct
     2221 gcaggatctc ggcgcggtcg acgtcacgcc gcttgttcgc cagccgcgcg ctagtgaagg
     2281 cgcggtcccg ctgctctatg agctcagcgt ccatggccac gagcacctcg cagcggcgct
     2341 gcttcgagcg ctctcggagg atgctctcgt cgagcttgca ctccaagatg cgccgctctt
     2401 cgcacacccg ttcatatgcc tcgctgcgat cctgctccac cgcgtcgcgc tcgcccagca
     2461 cgcgcagcac gtcggccgct tccatccagc gcccctcggg ggtgcgctcg atgtcgtagt
     2521 ccgcatcggg ctcggccccg aagcaatgca cgttgtatcg gttcagcgtt tccatgggtc
     2581 tttgcctttc gtcttcacgt cggacaggcc ttccttatcg gcccaccact ccagcacgat
     2641 cacattgccc tcgtgcccag gctgatacac ctcagagtca tcgtgcaact gcgagatggg
     2701 aatccagcgg ctctcgccct ccagctggat caggagggcg agccccgtcg agctgcgccg
     2761 cagcgccttg ccctggccga ggctcacccc atctgtcaca gctccacctg tggtgcgggt
     2821 tccgtgctcg gcgccggtgg cgggctccac ccggtcggtg gcgcgccagg gtcaacgaag
     2881 cggtttgctt cttcagcgat ggcgttctct gcttcctgcg agtagcagct tccaccgttg
     2941 atgcgctcgc ccatgatggc gagctcgatc agcttcgagt agcgccgcgc gtgctggtcg
     3001 tagcctgcca tagcgatgtt ggcccaccac gccgtcaacg tctcctcgtc cagcatcacc
     3061 ccgagcccgt tccggcggat gtactggagc agcagcgccg cgccgctctt ggcttcgaaa
     3121 ggcagctcac cggcggggat aggctcgacg ctggggtctg ccatcagtcg acgatagagc
     3181 tcggccagcg ccgtctcgat gttgatgtga gtttcgaact tcacccccag ccggtcgagg
     3241 cagcggaaca cgagccgcgt cgccttctcc agccgatcgt cgctctcgcg ttcacccttc
     3301 accgcgtcgt cgtgcatgag cgtgagctcc gagatgcgat cggtgagctc ctcgatcttc
     3361 tcgagcattg aatgctcgat gttctcgcgc agctccacgg ccacgtcgaa ggcctttccc
     3421 cagcgctccg ccctcacctc gagctcgtgc accaggtcgc tctgctgaag cagccgctcg
     3481 gtgagctgct cgcgttcgcg cgtggtccgg tcgagctgct cgacgtgctg cttccattgc
     3541 tcgttggggc acacgctgaa gccaagccgc gtcgcctcgc gcatgagagc gtgaaccgat
     3601 gcctctgtct gagcgttcac aggcgccccg tcttcaggtg aaaggttgcc agcgccgcga
     3661 acagctgctg tgcgattctg tcgctcttgg cgccctgccc ttcgagctcg cccgctgcgc
     3721 ggcgcacggc cgtgatgagc tcggtcaacg tgggatcgag ctcgacccgc accgcgagct
     3781 cctcgagctc caaatcgccc tcgggtgcct gcttgcaaat gttcggggct tgcccgccgt
     3841 gctgcaccca gtcgggatgc ctgttgtcgc agcgctgctg ctggtcttcg cgtgaaacta
     3901 cgtacatggg ttgtttgcct ttcgttcgtt agagaccaac ggagcccggg ctcgagggtt
     3961 gtcccgggct ccgttgctac gtgatgaggc cgtgcctctt gagctcggcg cgcccgctct
     4021 tggtgacgga ccagccgccg ccgggtcgca gctcggcctt tcccttcagc tgcagcgcgg
     4081 acacgtgccg ctgagcgcct gccttcgtga gttcgagcac ggaagcgatc tcgctcagtg
     4141 aagggtgctt gccctcgtgc tcgtgtgggt attcgaggag cgccaccaag acatcgcgct
     4201 cccgtcgggt tgcttcagcc atcgcccgta tgcatacccg tatgcatccg ccccgtcaag
     4261 ctcgaacgcg tgcgcgcagc gtggcgcgct gcgccatggc gatttgctac gcggccgctt
     4321 gaccgccacg tgtcgggctg gcacgtttcg ggagcgtcac cacgtcgccg ccccgggtag
     4381 ctcccggggc gactctcctc cgggagcgtg aaggcgcgga gagttccgac ggtagaaagg
     4441 gtctcacgtt cgtgagcgaa tacaggcatt tgcgcgatgc cgtctgggcg cgcgcatggg
     4501 ctggggcgtt gaccacgcct caattggtcg tggcgctccg cctcgtcgag catttcccca
     4561 ggctattccc tggtgaaaag acgctggcgc gctggacgca gctgggcgag cgcaccgttc
     4621 gcgatgcact gcgagcgctc gagg
//
LOCUS       DASUNT010000059         2752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_183482, whole genome shotgun sequence.
ACCESSION   DASUNT010000059 DASUNT010000000
VERSION     DASUNT010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183482"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFX94_01170"
     CDS             complement(<1..209)
                     /locus_tag="VFX94_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766818.1"
                     /translation="MTRKALQLDFVAPRRRSRLAGYLVLSASLVLAGGLVFKYGETRQ
                     RLYHLESAEALVGGARPARAIPRERL"
     gene            complement(206..802)
                     /locus_tag="VFX94_01175"
     CDS             complement(206..802)
                     /locus_tag="VFX94_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766819.1"
                     /translation="MSRLWPERLTIALEPLPPEEPQPWQGALEDLARRIAALRKRVDL
                     TVVLSNHFVRYAVLPGKEGAATPEEELALARFHFTKIHGERVKGWEVRVSEQLACAID
                     TALLEGIKSLFAPKGRARLVSVQPRLMAAYNGARRRIPREGAWLVLAEQGRTCLARVA
                     ARGWAWVHNGRETDADELIERERSRASGEPLPSLVLNL"
     gene            complement(783..2453)
                     /locus_tag="VFX94_01180"
     CDS             complement(783..2453)
                     /locus_tag="VFX94_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX5766820.1"
                     /translation="MIEAQKIAEARAEALAAAGSRRLVDVLEERLALEPDVFAERLAA
                     TFRYGVLRMDELRRLEPAFDLVPLSECSRKGCVLLRAGGGELLLATDDPFAAEQAAWA
                     AERISAPFAWRLVHRGDLVAYLASHEETLRALDAVSAEVGAAGDENAGAEDLSLKAIA
                     DEKSEVVRLVRSTLRDALKIGASDIHLESVAAGLAIKFRIDGILIPIKQIDDRAHAEQ
                     AIARVKVLAELDVTERRVPQDGRFKALDRGRAIDLRVSIMPSIHGEDAVLRVLDKQSL
                     YESTRQTLSLDSLGFEERDIKALRRLSHEPHGMLLVTGPTGSGKTTTLYAAITEINKG
                     QDKIVTIEDPVEYQLPGVLQIPVNEKKGLTFARGLRSILRHDPDRIMVGEIRDPETAN
                     IAVQSALTGHLVFTTVHANNVFDVIGRFMHMNVDLYGFVSALNAILAQRLVRLVCAHC
                     AEPEVPSADLLRESGLAREATRDWNFRIGKGCRECRGVGYRGRKAIAELLLLNDEMRE
                     RITSRAPIRTLKDTAAANGTRFLREAALEAVRRGETTLEEINRVTFVA"
     gene            complement(2450..>2752)
                     /locus_tag="VFX94_01185"
     CDS             complement(2450..>2752)
                     /locus_tag="VFX94_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5766821.1"
                     /translation="GRLAAASRAISEGRSVSESLEANGLATPVAMRMLVVGEQGGNMG
                     EMMDRAASFHDEELARWVDWFTRMFEPLLMAVIGLVIGAIVVLMYMPIFELAGNLR"
BASE COUNT          423 a          965 c          924 g          440 t
ORIGIN      
        1 aggcgctcgc gcgggatcgc gcgcgccggc cgcgcgcccc cgacgagcgc ctcggcggat
       61 tcaaggtggt agagccgctg gcgcgtttcg ccgtatttga agacaagccc gcccgcgagc
      121 acgagggaag cggacaggac caggtacccc gcgaggcgcg agcgccggcg cggggccacg
      181 aaatcgagct ggagcgcctt gcgcgtcata agttcagcac cagcgatggc agcggctcgc
      241 cgctcgcacg gctacgctcg cgctcgatga gctcgtcggc atcggtttcc ctgccgttat
      301 gcacccacgc ccagcccctg gccgcgacgc gcgcgaggca ggtacgcccc tgctcggcga
      361 gcacgagcca agccccctcg cgcggaatac gccgccgcgc gccgttgtac gccgccatga
      421 ggcggggttg caccgataca agcctcgctc ttcccttcgg ggcaaaaagg ctcttgattc
      481 cctcgagaag cgccgtatcg attgcgcaag caagctgctc ggagacgcgc acttcccatc
      541 ccttgacgcg ctcgccatgg atcttggtga aatggaatcg cgcaagggcc agctcctctt
      601 ccggcgtcgc ggcgccttcc ttgccgggca gcacggcgta gcgcacgaaa tgattcgaga
      661 gcaccacggt gaggtccaca cgcttgcgca gcgcggcgat gcggcgcgca aggtcctcga
      721 gtgcgccctg ccagggctgc ggctcctcgg gcgggagcgg ctcgagcgcg atggtgaggc
      781 gctcaggcca caaacgtgac acggttgatc tcctcgagcg tggtctcgcc acgccgcacc
      841 gcctcgagcg cggcttcgcg caggaaccgg gtgccgtttg ccgccgcagt gtccttcagc
      901 gtgcggatcg gcgcgcgcga agtaatgcgc tcgcgcatct cgtcgttgag gagcaggagc
      961 tcggcgatcg ccttgcggcc ccggtagccg acgccgcggc attccctgca gcccttcccg
     1021 atcctgaaat tccagtctct cgttgcttcc cgcgcgaggc ccgactccct caatagatcg
     1081 gctgaaggga cttccggctc ggcacaatga gcgcagacaa ggcgcaccag ccgctgcgcc
     1141 aggatcgcgt tcagcgccga gacgaagccg tagaggtcca cgttcatgtg catgaagcgc
     1201 ccgatcacgt cgaacacgtt attggcgtgc accgtggtaa agacgaggtg gccggtaagc
     1261 gcggactgca ccgcgatgtt ggcggtttcc ggatcccgga tctcgcccac catgatgcga
     1321 tcggggtcgt ggcgcaggat ggagcgcagg ccccgcgcga aggtgagacc ttttttctcg
     1381 ttcaccggga tctgcagcac gcccggaagc tggtattcca ccgggtcctc gatcgtcacg
     1441 atcttgtcct ggcccttgtt gatctcggta atggcggcat aaagcgtggt ggtcttgccg
     1501 ctgcccgtcg gcccggtgac gagcagcatg ccgtgcggct cgtgcgacag gcgccgcagc
     1561 gccttgatgt cgcgttcctc gaagccgagc gagtcgagcg agagcgtctg gcgcgtgctc
     1621 tcgtagagcg actgcttgtc cagcacgcgc aggaccgcgt cctcgccatg gatgctcggc
     1681 atgatggaca cgcgcagatc gatcgcgcgg ccgcggtcaa gcgccttgaa gcgcccgtcc
     1741 tggggcacgc ggcgctcggt gacgtcgagc tcggcgagca ccttgacgcg cgcgatcgcc
     1801 tgctcggcgt gcgcgcgatc gtcgatctgc ttgatcggga tgaggatgcc gtcgatgcgg
     1861 aacttgatgg cgaggcccgc ggcgacgctt tcgaggtgga tgtcgctcgc gccgatcttg
     1921 agcgcgtcgc gcagcgtcga gcgcacgagg cgcaccacct cgcttttctc gtcggcgatc
     1981 gccttgagcg agaggtcctc cgcgcccgcg ttctcgtccc ccgcggcgcc gacctccgcg
     2041 ctcaccgcgt cgagcgcgcg cagcgtctct tcatgactgg cgaggtacgc gacgagatcg
     2101 ccccggtgca cgaggcgcca ggcgaacggc gcgctgatgc gctccgcagc ccacgccgcc
     2161 tgctcggccg cgaacggatc gtcggtggcg aggagcagct ccccgcctcc ggcgcgcagc
     2221 agcacgcagc ccttgcgcga gcattcgcta agcggcacca ggtcgaacgc gggctcgagg
     2281 cggcgcagct cgtccatgcg cagcaccccg tagcggaacg tcgccgcgag ccgctcggcg
     2341 aagacatcgg gctcgagcgc gagcctctcc tcgagcacgt ccacgaggcg gcggctgccc
     2401 gcggcggcga gcgcctcggc gcgcgcttcg gcgatctttt gggcctcgat cacctgaggt
     2461 tcccggcaag ctcgaagatc ggcatgtaca tgagcaccac gatcgcgccg atcacgagcc
     2521 cgatcaccgc catgaggagg ggctcgaaca tgcgcgtgaa ccagtcgacc cagcgcgcaa
     2581 gctcctcgtc gtggaagctc gccgcgcggt ccatcatctc gcccatgttg ccgccctgct
     2641 cgcccaccac gagcatgcgc atggcgaccg gcgttgccag gccgttggct tcgagcgatt
     2701 ccgacacgct gcgcccctcg ctgatcgcgc ggctcgctgc cgccagacga cc
//
LOCUS       DASUNT010000060         3613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1060380, whole genome shotgun sequence.
ACCESSION   DASUNT010000060 DASUNT010000000
VERSION     DASUNT010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060380"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..2033)
                     /gene="asnB"
                     /locus_tag="VFX94_01190"
     CDS             complement(174..2033)
                     /gene="asnB"
                     /locus_tag="VFX94_01190"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX5766822.1"
                     /translation="MCGLAGYVGPQAVPDDVLARMTGRVAHRGPDAEGFYRAAPVALG
                     HRRLSVIDVAGSPQPMSTSDNGLTCVFNGEIYNFAAVRRELAAHGHRFRTNGDTEVLL
                     YAWREYGVRMLEHLQGMFAFALWDRSARRLFIARDHLGVKPLYYHWDGSTLVFGSELK
                     ALIEHPGVRRELDLDALGLYLECQYIPSPKTVYRGVRKLEPGHAIMVQEGKLSIERYW
                     RPDYSTKLKLDEPEALAQLEAQLRRSVESMLVSDVPLGSFLSGGVDSSLVSALMADIA
                     KRPIDTFTLGFYGESAASEHSHAELVSRHIGARNHVLMLDPSQMLAAFEDWTEAFDEP
                     FADPAALPTMLLAKLTRQHVTVVLTGEGADEVFSGYGNYRKRVSEEKVTSMLGARWSP
                     LRYLVRALPARARKDRLLKALAEPVSRRYVTIPMVFDPALHRDLLSPRFLSARETCMA
                     DYAERFFEECNSPQYIDKLMYIDARLWLPDDLLTKVDRATMAYSLEARVPYLDHRFFE
                     FCARLDPALKQRGRTGKYLLKKLAEKLLPGEIVHRPKQGFFPPLTEWFAGRLKAESQA
                     ALLRLDKRGLFRPGALSALATEHYSGRRNHSGRLWALLVLEKWFGRYVPEFSL"
     gene            2139..3608
                     /locus_tag="VFX94_01195"
     CDS             2139..3608
                     /locus_tag="VFX94_01195"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX5766823.1"
                     /translation="MRWILVATWTAAFFISAALFSNTVALRLLLLAAGLAFAALAVLQ
                     ERGSIHMLPPVWLAFVAWAAWAWLSLAWSIDPDRTLKDLRNETLYAGLAFWMCYVAGQ
                     ARASARVVVPVVAASAALLCLVALHAYVQGDLRSGERWHGGPGFLSAVLLTLLPCALA
                     GGWYGHRAGWRHARPVGVVLVLLFLAAAYSTFNRVVWLAAAVQVLVLGALLLVLGRTS
                     FAARTRTVVGAGVMIIAGAAAMGYVVQSAREVYYGGSIVLAEDPRLRLWPEIVERISA
                     RPITGYGFGRGMLGQPLSQELNDPLLWHAHNLFLDAALQLGLPGLLFLVFMLGATLRA
                     GWRLARSTDDYAAACGAAVVAVMAGMVVRNMTDTLWVRHTALVYWGVLGMLLAWGSAA
                     RQGKAAAKNLPMTRKDGNSPTTTPAQQRKSAPWHRACARRKSWPRNGGGRRRRAWCVT
                     PRPRAIARARAAARWNPLAARRARCLRRGRAPRCFPRGS"
BASE COUNT          584 a         1178 c         1225 g          626 t
ORIGIN      
        1 ggcgagcggg cgcgggtcga tgatggagag cctggcatgc accaggccgc tcctctccca
       61 gagctgcgcc cgctgcgcgt ccgggccgcg tgcccgcagc gccgcctgca tggcgccgat
      121 taccggggga ggtaccgtgc cctgcgcgaa gtagccgcct atcccgcaca tggctagagc
      181 gagaactccg gcacgtagcg cccgaaccac ttttccagca cgagaagcgc ccaaaggcgc
      241 ccggagtggt tgcggcgccc cgagtagtgc tccgtggcga gagcggacag tgcgccgggc
      301 cggaagaggc ccctcttgtc gagccgcagg agcgcggctt gcgactctgc cttgaggcgg
      361 ccggcgaacc actcggtgag cggagggaag aagccctgct tcggccggtg cacgatctcg
      421 cccggcagaa gcttctcggc aagcttcttg aggagatact tgccggtgcg gccgcgctgc
      481 ttgagggcgg ggtccagcct cgcgcagaac tcgaagaagc gatggtcgag gtacggcacg
      541 cgcgcctcta gcgagtacgc catggtggcg cggtcgacct tggtaaggag gtcgtccgga
      601 agccaaaggc gcgcatcgat gtacatcagc ttgtcgatgt actgcggcga attacattcc
      661 tcgaagaagc gctcggcgta gtccgccatg caggtctcgc gggcggaaag gaaccgcggc
      721 gagagaagat cccggtgcaa agccgggtcg aataccatcg gtatggtcac atagcggcgc
      781 gagacgggct cggccagcgc cttcagcagc cggtccttcc gcgccctggc gggaagcgcg
      841 cgcacgagat aacgcagcgg cgaccaccgg gcgccgagca tgctggtgac cttctcttcg
      901 cttacgcgct tgcgatagtt accgtagccg gagaacacct catcggcgcc ctcgccggtc
      961 agcaccacgg tgacatgctg gcgcgtgagc ttcgcaagga gcatcgtcgg cagcgccgcc
     1021 ggatctgcga acggctcgtc gaacgcttcg gtccagtcct cgaacgccgc gagcatctgc
     1081 gaaggatcca gcatgaggac atggttcctc gccccgatgt ggcgcgaaac gagctccgcg
     1141 tgcgaatgct cgctagcagc gctctcgccg taaaaaccga gcgtgaaagt gtcgatcggc
     1201 cgcttcgcga tgtccgccat gagagcgctc accaggctcg agtccacgcc gccggacaag
     1261 aagctgccga gcggcacgtc ggagaccagc atgctttcca ccgatcgacg cagctgcgct
     1321 tcgagctgcg caagcgcttc gggttcatcc agcttcaact ttgtggagta gtccggacgc
     1381 caatagcgct cgatggagag cttgccctct tgcaccatga tcgcgtggcc cggctcgagc
     1441 ttgcgcacgc cgcgatacac ggtcttcggc gaaggaatgt actggcattc gaggtacagc
     1501 ccgagcgcgt caaggtcgag ctcgcgccgc acgccgggat gctcgatcag cgccttgagc
     1561 tccgagccga agacgagcgt tgatccgtcc cagtggtaat agagcggctt tactcccagg
     1621 tggtcccgcg cgatgaagag ccgccgcgcg ctccgatccc acagcgcgaa ggcgaacatg
     1681 ccttgcaggt gctccagcat gcgcacgccg tactcgcgcc aggcgtagag caggacttca
     1741 gtgtcgccgt tggtcctgaa gcggtggccg tgcgcggcaa gctcccggcg cacggccgcg
     1801 aaattgtaga tctcgccgtt gaacacgcag gtcagcccgt tgtcggacgt gctcatcggc
     1861 tgggggctgc ctgccacgtc gatgaccgag agccggcgat ggccgagcgc caccggcgca
     1921 gcgcgataga agccctcggc gtcggggcct cggtgggcaa cccgccccgt catgcgcgca
     1981 agcacgtcgt ccgggacggc ctgcgggccc acgtaaccgg cgaggccgca catcctgaac
     2041 ctattctatg cgatcggggg tcgtgggcac gagaccaccg cgctgccagc gccttccact
     2101 tgactgttca agctcgggac gtcataagcc atcgcgaaat gcgctggatc ttagtcgcga
     2161 cgtggactgc agcgttcttc ataagcgccg cacttttctc gaacaccgtc gcgctgcggc
     2221 tcttactact cgccgcgggc ctcgccttcg cggcgcttgc cgtgctccag gaacgaggct
     2281 cgatccacat gctgcctccc gtgtggctgg ccttcgtcgc ctgggccgcg tgggcatggc
     2341 tatcgcttgc gtggtcgatc gatcccgatc ggaccctgaa ggacctgcgc aacgagacgc
     2401 tttacgccgg cctcgctttc tggatgtgct atgtggcggg ccaggcgcgc gcgtcggcgc
     2461 gcgtcgtcgt tcctgtcgtc gctgcgagcg cggcgctcct gtgcctggta gccctgcacg
     2521 cctacgtgca gggagacctg cgctccggcg agagatggca tggaggaccg gggtttcttt
     2581 ccgcggtctt gctgacgctc ctgccgtgcg cgctggcggg cggctggtac ggacaccgcg
     2641 cgggctggcg gcacgcgcgg ccggtcggcg tagtcctcgt gctgctattt cttgccgccg
     2701 cttattccac tttcaaccgg gtagtgtggc tcgccgccgc cgtacaggtg ctggtcctcg
     2761 gcgcgctcct tctcgttctc ggcagaacat cattcgccgc gcgcacccgt accgtcgtag
     2821 gggcaggcgt catgataatc gcaggtgccg cggcgatggg atatgtcgtt cagtcggcgc
     2881 gcgaggtgta ctacggcggc tcgatcgtac ttgccgaaga cccgaggctc aggctctggc
     2941 ccgagattgt ggagcgcata agcgcacggc ccatcaccgg ctacggattc gggcgcggca
     3001 tgctggggca gccgctgagc caggagctga acgatccgct cctttggcac gcccacaatc
     3061 ttttcctcga tgcagccctg cagctcgggc tgccggggct gcttttcctt gttttcatgc
     3121 tgggagcaac gttacgcgcg ggctggcgcc tggcccgttc aacggacgac tacgcggccg
     3181 cgtgcggcgc cgcagtggtt gccgtgatgg ctggaatggt ggttcgcaac atgaccgaca
     3241 cgctgtgggt ccggcacact gcgctcgtgt actggggagt cctcggaatg cttctcgcat
     3301 ggggatcggc agcgcgccaa gggaaagcgg cggcgaagaa ccttccgatg acgcggaaag
     3361 acggtaattc gccgacgact acccccgcac agcagcgtaa atccgctcca tggcatcgag
     3421 catgcgctcg taggaaaagt tggccgcgca atggcggcgg gcggcggcgc cgagcctggt
     3481 gcgtaacgcc tcgtccgcga gcaatcgcgc gagcgcgcgc tgcagcgcgg tggaatcctt
     3541 tggcggcacg acgagcgcgg tgtctccgtc gcgggcgagc tccgcgatgc ttcccgcgtg
     3601 ggtcgtgacg cac
//
LOCUS       DASUNT010000061         4592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1148262, whole genome shotgun sequence.
ACCESSION   DASUNT010000061 DASUNT010000000
VERSION     DASUNT010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148262"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..750)
                     /locus_tag="VFX94_01200"
     CDS             complement(124..750)
                     /locus_tag="VFX94_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5766824.1"
                     /translation="MPTDSPIAALDDGALARRIAGAGAAPDTAAEAELYRRLAPRVRL
                     YGLRHLRDRQAAADLVQQVLLMTLERLRAGEVREPERIASFALGACRMTVLELRRGTR
                     RREELLEKWVPAYAGTTDSNGVPAYAGMAYEAPEPLVLDPERLAACLEALAERERSVV
                     VMSFFADKPADEVGAELGLTGGNVRVIRHRALGRLRECMGPRLRGGDK"
     gene            864..1688
                     /locus_tag="VFX94_01205"
     CDS             864..1688
                     /locus_tag="VFX94_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5766825.1"
                     /translation="MITNTTSGTNLHEIARGIYRINTPLALPDGAGFSFNQYLVADEE
                     PLLFHTGPRRMFPLVAEAITRILPLAALRYIAFSHFEADECGSLNDFLAAAPQAEPVC
                     SRVAAMVSVNDIASRPARALADGEVLRTGRHALKWLDAPHVPHAWENGFMMELETRTL
                     LCGDLFTQGGSGAAAITESDILGPSEAFRQPMDYYAHAPQTGATLEKLAREEPQVLAC
                     MHGSAWRGDGASLLRALARALTRAHHVQRQEAELDSGAVPIASVSTANAAAPRPRA"
     gene            1706..2470
                     /locus_tag="VFX94_01210"
     CDS             1706..2470
                     /locus_tag="VFX94_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5766826.1"
                     /translation="MARARQPSGLDPRQNRLLAALPEAEFQHLAPALEPVALTIGTAL
                     YESGRPQPYLYFPASGIVSLLYLLEDGASTEIALTGREGVVGVSLFMGGGATPSRAVV
                     QIVGHAWRMRAAAMKRSFDRGEALQGLLLRFTQALITQMTQTAVCNRHHALDQQVCRW
                     LLFALDRLGSSEVRMTQERIAHLLGVRREGVTEAAGRLQAAGAIEYSRGRIRVLERGA
                     LESRACECYAVVTAEYERLLPALRPATGGSPPAARS"
     gene            complement(2559..2723)
                     /locus_tag="VFX94_01215"
     CDS             complement(2559..2723)
                     /locus_tag="VFX94_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766827.1"
                     /translation="MKYRTAVKTGRTAAMQADGLRPGSFVSYAAMVERLSLGYIRPLV
                     LRHRAPKPGP"
     gene            2861..3130
                     /locus_tag="VFX94_01220"
     CDS             2861..3130
                     /locus_tag="VFX94_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766828.1"
                     /translation="MEILVWIAAGILLGWLSYSLLGFNAKRGMVTSMLIGAVGALIGA
                     KAVAPVFVSAASAPDEASLPFVLFAAGAAAVLLALGDVLHQRFGV"
     gene            3136..3345
                     /locus_tag="VFX94_01225"
     CDS             3136..3345
                     /locus_tag="VFX94_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766829.1"
                     /translation="MTFQDEIAEVQARIAGARAERDAGQAAGQPHKYFDACCALERLE
                     RELEGLRQQGLRAFASRHAIADLGI"
     gene            complement(3729..4085)
                     /locus_tag="VFX94_01230"
     CDS             complement(3729..4085)
                     /locus_tag="VFX94_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX5766830.1"
                     /translation="MRRRLAKGDPAPESDDGARLELLAKLIDDYERERFKFRKPDAID
                     AIVFRMEEQGLRQADIAAIVGGKNRASEILSRKRPLTLSMVRALHEKLGIPSELLIRE
                     PAAEHRVRRRGGKRTR"
     gene            complement(4108..4335)
                     /locus_tag="VFX94_01235"
     CDS             complement(4108..4335)
                     /locus_tag="VFX94_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766831.1"
                     /translation="MKTRRAPRVEQMRAEYDFDYSKAERGKYFKRLLKEGSNVVVLDR
                     DVAKAFPDSAAVNKALRSVMKSRKPRRVSRS"
     gene            complement(4325..>4592)
                     /locus_tag="VFX94_01240"
     CDS             complement(4325..>4592)
                     /locus_tag="VFX94_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEX5766832.1"
                     /translation="HFVWHRKKAEANLRKHKVAFEEAVSVFYDPLSATFRDPGASGGE
                     ERFVTIGHSSRQRLLVVLHTEEDETIRIISARPATVHERKRHEN"
BASE COUNT          662 a         1643 c         1532 g          755 t
ORIGIN      
        1 cgtgcagtgc gcgcagccga agaggtgctc ctcgatcctt tcctcctcgc cggctgcgag
       61 ctcgcccagc cagtaggcga cgaggtactc gaaattgatc ggttccttac acatcggcgc
      121 cgctcatttg tcacccccgc gtaggcgagg acccatgcac tcccgcagcc gtcccagcgc
      181 gcggtgacgg atgacgcgga cgttgccgcc cgtgagcccc agctccgcgc ccacctcgtc
      241 ggccggcttg tcggcgaaga aactcatcac caccaccgag cgctcgcgct ctgcgagcgc
      301 ctccaggcac gcggcgaggc gctcgggatc gaggaccagc ggctccggcg cctcataagc
      361 catccccgcg taggcgggga ccccatttga gtccgtcgtc cccgcgtagg cggggaccca
      421 tttctcgagc agctcctcgc gccgcctggt gccccgccgc agctcgagca ccgtcatgcg
      481 gcaggcgccg agcgcgaacg aggcgatgcg ctcgggctcg cgcacttcgc ccgcgcgcag
      541 gcgctcgagc gtcatgagca gcacctgctg cacgagatcc gccgccgcct gccggtcgcg
      601 caggtgccgc agcccgtaga gacgcacccg cggcgcaagc cgccggtaga gctccgcctc
      661 ggcggcggta tcgggcgctg cgcccgcgcc ggcgatgcgg cgcgcgagcg cgccatcgtc
      721 gagcgcggcg atcggggaat ccgttggcac gggccgagca taaccggatg tgtcaccacc
      781 gcccgcttcg ttacacgggg cgtttcggtg taacgcttct cgtcccggcc ccactaccct
      841 gctatctcaa cccggagact tccatgatta ccaacacgac cagcggcacc aacctccacg
      901 aaatcgcgcg cggcatctac cgcatcaata cgcccctcgc cctccccgac ggcgccggct
      961 tcagcttcaa ccagtacctc gtcgccgatg aggagccgct cctcttccac acgggcccgc
     1021 ggcgcatgtt cccgctcgtc gccgaagcta ttacgagaat ccttccactg gcggcgctgc
     1081 gctatatcgc cttctctcac ttcgaggccg acgagtgcgg ctcgctcaac gacttcctcg
     1141 ccgccgcgcc gcaggccgag ccggtctgca gccgcgtcgc cgccatggtg tcggtgaacg
     1201 acatcgcctc gcgccccgcc cgcgcgctcg ccgacggcga agtcctgcgc accggccgcc
     1261 acgcgctcaa atggctcgac gccccgcacg tgccgcacgc gtgggaaaac ggcttcatga
     1321 tggagctcga gacccgcacc ctgctctgcg gcgatctctt cacgcagggc ggctccggcg
     1381 ccgcggcgat caccgagagc gacatcctcg gcccgagcga ggcgttccgg cagccgatgg
     1441 attactacgc gcacgccccg cagaccgggg cgacgctgga gaagctggcg agggaggagc
     1501 cgcaggtgct cgcgtgcatg catggcagcg cctggcgcgg ggatggagct tcgctcctgc
     1561 gcgcgctggc gcgggcgctg acgcgcgctc accacgtcca gcgtcaagaa gcggagctcg
     1621 attctggcgc ggttccgatc gcgtccgtct cgaccgcgaa cgccgccgcg ccgaggcccc
     1681 gcgcgtagcg ctgtaccatc ggcgcgtggc gcgcgcgcgg cagccttccg gcctggaccc
     1741 gcggcagaac cggctgctcg cggcgctgcc ggaagccgag ttccagcacc tcgcacccgc
     1801 cctcgagccg gtggcgctca ccatcggcac ggcgctgtac gagtccgggc ggccgcagcc
     1861 ttacctctat ttcccggcga gcggcatcgt ctcgctgctc tacctcctcg aggacggcgc
     1921 ctccaccgag atcgcgctca ccggccgcga aggcgtggtc ggcgtgtcgc ttttcatggg
     1981 cggcggcgcg acgccgagcc gcgcggtggt gcagatcgtc gggcacgcct ggcgcatgcg
     2041 cgccgcggcg atgaagcgaa gcttcgaccg cggcgaggcg ctgcaggggc tgctcctgcg
     2101 ctttacccag gcgctcatca cgcagatgac gcagaccgcg gtgtgcaacc ggcaccacgc
     2161 gctggaccag caggtgtgcc gctggctcct cttcgcgctc gaccggctcg gctcgagcga
     2221 ggtgcgcatg acgcaggagc gcatcgccca tctcctcggc gtgcgccgcg aaggggtgac
     2281 cgaggcggcc ggcaggctcc aggccgccgg cgcgatcgag tacagccgcg gacgcatccg
     2341 cgtcctcgag cgcggcgcgc tcgagagccg cgcctgcgag tgctacgcgg tggtcacagc
     2401 tgaatacgag cggctgctgc cggcgctcag gcccgcgacg ggcggttcgc cacctgcagc
     2461 ccgttcctga cgcgaggtcg cggcctcgcg cgatcgcgct ctggacccgc tgagtgatgg
     2521 cgccgtcgag gagcgggccg cgaagacggg caggcggctc acggcccggg cttcggcgcg
     2581 cgatggcgca gcacgagcgg ccggatgtag ccgagggaga ggcgctcgac catggcggca
     2641 tagctgacga agcttcccgg gcgcaagcca tcggcctgca tggcggcggt gcggccggtc
     2701 ttgacggcgg tgcggtattt catttgggtg atttccttgg catgttgtcc ggccgagcgt
     2761 gcgcggttgc gagcgcacgc tctgtgcgct ggctcgcaca tgcgtcccta agttggccag
     2821 cgcacggatg cgcggcgccg aaaggacgag aatccggccc atggaaatcc tcgtatggat
     2881 cgcggccggg atccttctcg gctggctgag ctactcgctc ctgggcttca acgcgaaacg
     2941 cggcatggtg acgtcgatgc tgatcggcgc cgtgggcgcg ctgatcggcg cgaaggccgt
     3001 tgcgccggtg ttcgtcagcg cggcgagcgc cccggacgag gcgagcctgc ctttcgtcct
     3061 cttcgccgcc ggcgccgcgg ccgtgcttct cgcgctcggt gacgtgctgc accagcgctt
     3121 cggcgtgtag cgacgatgac cttccaggac gagatcgccg aggtccaggc gcgcatcgcc
     3181 ggcgcgcgcg ccgagcgcga cgcgggccag gccgcggggc agccgcacaa gtacttcgat
     3241 gcctgctgcg cgctggagcg gctcgagcgc gagcttgagg gactgcgcca gcagggcctg
     3301 cgcgcttttg cgagccgcca cgcgatagcc gacctcggca tctagcttcg cgacggcgcc
     3361 taccagttcg acgggcgccg ctacctgcgc ctcgccgacg cgctgagcga cgcgcaatcg
     3421 atgcgcgtgc ggcccacgct tgctgcagcc gcctaagccc gtctcgtagt cgtcgatcag
     3481 ctttggattc aggtttgggg gtctggcccc gaacttccgg gcttatcggt ttgggggtct
     3541 gaccccgaac tttctaccga ccacgacttc gagatctatc gcacgcgcac ccaggagcgg
     3601 ctggagctgg ttctgcagct gtagcgcccg ccctgaggca cccctcgcgt gagggccctg
     3661 gggtcagacc ccgaatctgt tccagctgcg cgtcagcagg ttgggggggc tgaccccgaa
     3721 ctttgggttt atcgagtccg cttgcctccg cgtctgcgca cgcggtgttc ggcggcgggc
     3781 tcgcggatca gaagctcgct cggaatgccg agcttctcgt gaagtgcacg aaccatcgaa
     3841 agcgtgagag gccgcttgcg tgaaaggatc tcggaggccc ggtttttccc gccgacgatc
     3901 gcggcgatgt ccgcctggcg caggccctgc tcctccatcc ggaagacaat cgcatcgatc
     3961 gcgtcaggct tgcggaactt gaagcgctct ctctcgtagt cgtcgatgag cttcgcgagg
     4021 agctcgagcc gtgcgccgtc gtcgctttcc ggcgccgggt cgcccttcgc aaggcgccgc
     4081 ctcattcggt cgggctcggg tgcccgacta cgatcgtgag acccgccggg gcttgcggct
     4141 tttcatcacc gagcgcagcg ccttattcac cgcagcggaa tcgggaaaag ctttggcgac
     4201 gtcgcggtcc agcaccacca cgttcgaccc ttccttcagc agccgcttga agtactttcc
     4261 ccgctcggcc ttggaatagt cgaagtcgta ttccgcgcgc atctgttcga cccgtggggc
     4321 tcgcctagtt ttcatgccgc ttgcgctcat ggaccgtcgc aggacgtgca ctgatgatcc
     4381 tgattgtctc atcttcctcc gtatgcagaa cgacgagcaa tcgctgacgc gaagagtggc
     4441 cgatcgttac gaaccgctct tcgccgcccg aggcgccggg atcgcggaat gtcgccgaga
     4501 gcgggtcata aaacacactg accgcctcct cgaaggccac cttgtgcttc ctgaggttcg
     4561 cctcggcttt tttccgatgc caaacgaagt gc
//
LOCUS       DASUNT010000062         2311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_972679, whole genome shotgun sequence.
ACCESSION   DASUNT010000062 DASUNT010000000
VERSION     DASUNT010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972679"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /gene="gatB"
                     /locus_tag="VFX94_01245"
     CDS             complement(<1..307)
                     /gene="gatB"
                     /locus_tag="VFX94_01245"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206853.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEX5766833.1"
                     /translation="MTWEIVVGIETHAQLLTRSKIFSGAATAFGAPPNTQASAVDIAL
                     PGVLPVLNGAAVEHAIRFGLAVNGTINRRSVFARKNYFYPDLPKGYQISQYELPIVQG
                     "
     gene            complement(304..1770)
                     /gene="gatA"
                     /locus_tag="VFX94_01250"
     CDS             complement(304..1770)
                     /gene="gatA"
                     /locus_tag="VFX94_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX5766834.1"
                     /translation="MLNSSLTELAAALAARNISSVELTSFFLDRIGKLNGELNAFIWP
                     EKTRDIALQQARRADEKLSSGEGEPLTGIPIAFKDIFCTKGIHTTCGSRMLESFVSPY
                     DAHVVEQFARAGAVLLGKCNMDQFAMGSSNESSYFGPVRNPWARKRVPGGSSGGSAAA
                     VAARMAPAATGTDTGGSTRQPAAFSGICGLRPTYGLVSRFGLVAFASSLDQAGPLARS
                     AADLAWLLNVMAGYDPRDSTSIDRPKEDYTKALNAQLKGLRIGIPREYFAEGIEPGVR
                     VAVEQAISWFTAHGAVRVELQLPLTGLAVPAYYVIAPAEASSNLSRFDGVRYGHRAEQ
                     YEDLADMYCRSRAEGFGDEVKRRILVGTYVLSHGYYDAYYLQAQKVRRLIAQDFSRAF
                     ESCDLIAGPTAPTTAFEIGAKADDPVQLYLGDIFTVPAPLAGLPALSIPCGFDGAGLP
                     VGLQLMGRHFSEGLLLGAAHRYQQASDWHLRVPAETPL"
     gene            complement(1788..2075)
                     /gene="gatC"
                     /locus_tag="VFX94_01255"
     CDS             complement(1788..2075)
                     /gene="gatC"
                     /locus_tag="VFX94_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004574467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX5766835.1"
                     /translation="MSLSDDQVRRLAGLARIAIRPEEFEPVLERLNRVLALVDEMLRV
                     DAAGVEPMAHPLDARQPLRADAVTESDQRELYQSVAPAVEGGLYLVPKVIE"
BASE COUNT          406 a          760 c          758 g          387 t
ORIGIN      
        1 cgccctggac aattggcagc tcgtactggc tgatctgata gcccttgggg aggtccggat
       61 aaaaataatt cttgcgcgcg aataccgagc ggcggttgat ggtgccgttc actgcaaggc
      121 cgaagcggat ggcatgctcg accgccgcgc cgttgaggac cggcagcacg ccgggaaggg
      181 cgatgtcgac cgcggacgcc tgggtgttcg gcggggcgcc gaaagcggtg gccgcgccgg
      241 agaagatctt gctccgcgtg aggagctgcg cgtgcgtctc gataccaaca acgatttccc
      301 acgtcacagc ggtgtctccg ccgggacgcg caagtgccag tcgcttgcct gctggtaacg
      361 gtgcgctgcg ccgagcagga gcccttcgga gaagtgccgg cccatcagct gcagccccac
      421 cggcagcccc gcgccgtcga agccgcacgg gatcgagagc gcgggcagcc cggcgagcgg
      481 cgccgggacg gtaaagatgt cgccgagata gagctgaacc ggatcgtcgg ccttggcgcc
      541 gatctcgaac gcggtcgtcg gcgctgtcgg accggcgata aggtcgcagg actcgaacgc
      601 ccgggagaaa tcctgcgcga tcaggcgccg caccttctgc gcctgcaggt aataggcatc
      661 gtagtagcca tgcgacaaca catacgtacc gacgagaatg cggcgcttca cctcgtcgcc
      721 gaagccctcg gcgcgcgagc ggcagtacat gtctgccagg tcctcgtact gctccgcgcg
      781 gtgcccatag cgcacgccgt cgaagcgcga aaggttcgac gaggcttccg caggcgcgat
      841 gacgtaatac gccggcaccg cgaggcccgt gagcggcaat tgcagctcca cacgaactgc
      901 gccatgcgcg gtaaaccacg atatcgcctg ctccaccgcc acgcgtacgc ccggctcgat
      961 gccttcggcg aagtactcgc gcgggatgcc aatgcgcaat cccttgagct gggcgttcaa
     1021 cgcctttgtg tagtcttctt tcggccgatc gatgctggtc gaatcgcgcg ggtcgtaacc
     1081 tgccatcacg ttgagaagcc aggcgaggtc ggccgcgctg cgcgcgagcg gcccggcctg
     1141 gtcaagggag gaggcgaacg ccaccaggcc gaagcgcgag accagtccgt acgtcggccg
     1201 aagcccgcaa atgccgctga atgccgccgg ctggcgcgtc gagccgccgg tgtccgtgcc
     1261 ggttgcggcg ggcgccatgc gcgcggcgac cgcggccgcc gagccgcccg aggagccccc
     1321 gggaacgcgc tttcttgccc acggattgcg taccggcccg aagtacgagc tttcgttgga
     1381 ggagcccatg gcgaactggt ccatgttgca ctttccaagg agcacggccc ccgcccgggc
     1441 gaactgctcc acgacatgcg cgtcgtaggg gctcacgaag ctctccagca tcctcgagcc
     1501 gcaggtcgtg tggatgccct tcgtacagaa gatgtccttg aacgcgatcg ggatgcctgt
     1561 tagcggctcg ccctcaccgc tggaaagctt ttcatctgcg cggcgcgcct gctgcagggc
     1621 gatgtcccgc gtcttctcag gccagatgaa agcgttcaac tcgccgttca gcttgccgat
     1681 ccggtccagg aagaagctgg tgagctcgac ggaggaaata tttctcgccg ccagagcggc
     1741 ggcgagctcg gtgaggctcg aattaagcac ctaccctgct cggcctttca ttcgatcacc
     1801 ttggggacga ggtacagccc cccttcgacg gccggggcaa ccgactggta gagctctcgt
     1861 tggtcggatt cggtcacggc gtccgctcgc agaggctgcc ttgcgtcaag cgggtgcgcc
     1921 atcggctcga cgccggcggc gtccacccgg agcatctcgt ccaccagggc gagcacgcgg
     1981 ttcaggcgct cgagcaccgg ctcgaactcc tcggggcgga tcgcgatgcg cgcgagcccg
     2041 gcgaggcgcc gcacctggtc gtctgatagc gacattcttt cggacctgac gccggggaag
     2101 cattgcctta tctcccgcag cggcgttagc gtatcatagc gagccattga aacggccttc
     2161 agggattcac ctaaggcccg gatcgctaac gataaatcgg ctagtacgga gaaaggctcc
     2221 accttgccca tgcttggatt cctgcgcagc tacttttcca acgaccttgc gatcgacctc
     2281 ggcaccgcca acacgctgat ctacgtgcgc g
//
LOCUS       DASUNT010000063         8657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_446982, whole genome shotgun sequence.
ACCESSION   DASUNT010000063 DASUNT010000000
VERSION     DASUNT010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8657
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446982"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFX94_01260"
     CDS             complement(<1..466)
                     /locus_tag="VFX94_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX5766836.1"
                     /translation="MFDQFIGTKPVEERHRIDVRRLEKFLGFSIATLEQFKGGQSNPT
                     YKLASADGRGFVLRRKPPGKLLPSAHAVEREYRVISALHKAGFPVARPHVLCEDDAVI
                     GTAFYVMDYVEGRVLWDQSLPGMSKAERAAIWDELNRVIANLHSLDYRALGLE"
     gene            complement(497..952)
                     /gene="dtd"
                     /locus_tag="VFX94_01265"
     CDS             complement(497..952)
                     /gene="dtd"
                     /locus_tag="VFX94_01265"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX5766837.1"
                     /translation="MIALIQRVSGANVRVGGEPVGVIGAGIVALVGVERADTEVQALL
                     LAERVASYRIFADGAGKMNLSLKDVRGEILAVPQFTLAADTTSGLRPSFSSAALPAQA
                     EPLFNRFAAKLHDWSPRVQTGRFGADMQLSLVNDGPVTFWLRIAPGNSS"
     gene            complement(949..1116)
                     /locus_tag="VFX94_01270"
     CDS             complement(949..1116)
                     /locus_tag="VFX94_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX5766838.1"
                     /translation="MALEVEHHDLDDVIGRLVIDPTQDQLQLRRLKKRKLLLKDQIQR
                     LRARLIPDIIA"
     gene            complement(1284..2249)
                     /locus_tag="VFX94_01275"
     CDS             complement(1284..2249)
                     /locus_tag="VFX94_01275"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEX5766839.1"
                     /translation="MRRFFFMVLFQASIALAQDKPDETLNAVVGVSAKIQANARSAET
                     LGLQRQGTGVLVRNGYALTIGYLVIEAESVQVTGADGRTLPATLAGYDHASGFALLKI
                     VGAMGAKPLPLGDSAALAERDPAMAVTASARDSPTLVYVVSRRPFSGSWEYLLDSAIY
                     TYPPVMDWSGAPLIGPKGELLGIGSLIVGDAGAPGTQSPGNMFVPIDLLKPILDELIA
                     NGKRSGPARPWLGVNAEEARGRLFVARVSADGPAARAGLKSGDIILAVGADEVATLAD
                     FYRKVWGKGAAGTAVPLKVLQGARVRDVTVHSIDRIEYFRPSKTY"
     gene            complement(2263..2646)
                     /locus_tag="VFX94_01280"
     CDS             complement(2263..2646)
                     /locus_tag="VFX94_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766840.1"
                     /translation="MAKGFEGPDTEALPSRLLVGQSIQTRSRRWKKRSSMGCEKWGTS
                     LGETWSSICVTRAGITVATYGPNWQKTYRCAATFVDRILKVAKPADMPIEQPSIEVVV
                     NLRTSREIGVSIPQSILVRAERILE"
     gene            2763..3377
                     /locus_tag="VFX94_01285"
     CDS             2763..3377
                     /locus_tag="VFX94_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766841.1"
                     /translation="MFLALLCGTLALGAGLLAAAGYSEGTVVAARTAALTIGWLLLVA
                     TLVGMQGVRRRRPAEHATTVVVFVSGIGVLYLCYFHWAQLPQPAVRLADGPIVHEPAL
                     ETELVAEPLVASAIVVSPRVVAQVTSAPIVEKPRIETEPIVDRCSSLGGVEATQCSRC
                     LGKSGIARMMCLESARLVYCEGRPTDEVCPSPIPAATSEYVAPG"
     gene            3432..4241
                     /locus_tag="VFX94_01290"
     CDS             3432..4241
                     /locus_tag="VFX94_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5766842.1"
                     /translation="MRLISWNVQWCRGMDGRVDPKRIAAEARRLADPDVLCLQEVACN
                     FPEMEGSAGEDQVLELTRSLEGYEGFFAPAVDIPGKKGRRRFGNLVLTRLPAGRVMRH
                     SLPWPASPGVPSMPRAVVEVVVDSGLGPVRVMTTHLEYYSAEHRDAQIGRLGEIHLEA
                     CAAHLRVDEPGSFESHERPSSAVLCGDFNLKPDDPLHRKILDAGFADAWQALHPGKPH
                     PHTFKLYDKNEKPYCCDYVFVTRELAPRLRAIRIDSQTQASDHQPVMVELG"
     gene            4234..5070
                     /locus_tag="VFX94_01295"
     CDS             4234..5070
                     /locus_tag="VFX94_01295"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX5766843.1"
                     /translation="MAEIRKIGEALGAEVSGVHLSQPIAAETFARIRAAWLEHMVLRF
                     RGQKLTDSQLLAFSRQFGELDPPGPNPLGRPFLPDHPEMNVISNIKEGGVPIGGLGDG
                     EAIWHADMTYIERPPMAAILYAIEIPPAGGDTYWANMVLAYETLPAKLKARIEGREAV
                     HDATYNSAGTLRKGYKQVIDPRTAPGARHPLVRMHPESGRRCLFLGRRRNSSIVGLEL
                     HESERLLDELWRHATQPQFTMRQQWRVGDVMVWDNRCTLHRRDGFDPAARRLLHRTQI
                     RA"
     gene            complement(5060..5443)
                     /locus_tag="VFX94_01300"
     CDS             complement(5060..5443)
                     /locus_tag="VFX94_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEX5766844.1"
                     /translation="MFDWRRPTNLGVKDGLLAPCRRSPNCVSSQADPSDREHYIAPLK
                     ASMEDLRRAVEALPRTHIVRAHPNYLYAEFRSRLLRFVDDVEFFFDGAAIQVRSASRL
                     GRRDFGVNRSRIEKIRRLLAARSTP"
     gene            complement(5443..6249)
                     /locus_tag="VFX94_01305"
     CDS             complement(5443..6249)
                     /locus_tag="VFX94_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5766845.1"
                     /translation="MDYGAYQDLKIHRPEAGILEIVMGEEGRLAVATARAHAEMARVW
                     LDVDRDPETRVALLRGAGKGFSAGGDMAMVEEMTRDFAVRARVWREARDLVYNMLNCS
                     KIIISAIHGPAVGAGLAAGLMADISIAAKSARLIDGHTRLCVAAGDHAALVWPLLCGM
                     AKAKYYLLTCDTISGEEAERLGLVSLSCEESELRDRALEVARKLAAGPQTALRWTKYS
                     LNNWLRMAGPAFDASLGLEMLGFTGPEVKEGLASLREKRDPRFPPEPQDF"
     gene            6276..8183
                     /locus_tag="VFX94_01310"
     CDS             6276..8183
                     /locus_tag="VFX94_01310"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766846.1"
                     /translation="MLRRLFGGSRAQDNSRELLAQAYDSFKAGAYDDAERLCAACLDA
                     AADFVEAHHLHALIALARGELEPAFTRLKLAAGLDDSGSPFHGALGSALQYFEAHEAA
                     AHHLARALDLARREDPARAELMLCLSDSLIARKEFQQAERVLRELLSAIPEHPEALSR
                     LASVRFYESDAREARSLMDRYLAARPEAGLRLRRALMMPVILESNEEIDALRSRLERD
                     LDELGEARLAPIRDPVSEVGLTAFYVAYHGRRNRDLLRKFGRVCRALYPARAEAPKRS
                     PAGRRRLRIGFVSTFFFTHSVGRTTLGLIKDLPRSDFEVHVFAVAPHEDEMAREIRLA
                     AERYAALPRNLEQVRAAIEAAELDILLFADIGMEPLTTFLSLWRLAPLQLNTWGHSVT
                     SGIDTVDYYVSGESVEAAGAQELYSEKLMRLPGYFMPRYHRPPALPRKSREELGLPAD
                     RHLYFSPQSLFKLHPDFDAALHGILEHDPRAEIVLSDSRASWKELLRGRLARRLGKNA
                     DRVRFMPSVPQREFQQYLAAADVIIDPFHFGGCNTSCEALGLGIPIVTLPAFQLPGRF
                     TLGLYRELGLDACIAGSESEFVELAVRVGTEPDLRRSICAQIAERSERLFERPDTGRA
                     LGEALLRIARR"
     gene            8186..>8657
                     /locus_tag="VFX94_01315"
     CDS             8186..>8657
                     /locus_tag="VFX94_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5766847.1"
                     /translation="MLAFDDIARAHERIRPQVKRTPVLTSATVDALTGAAVFFKCENF
                     QRMGAFKFRGAYNALSQLDTGRKRQGVVAFSSGNHAQAVALAGKLLGIKTVIVMPQDA
                     PRVKLEATRGYGAEVVQYTRHEDRETLATRLARERGLTLVPPFDHEHIIAGQGTA"
BASE COUNT         1416 a         2959 c         2844 g         1438 t
ORIGIN      
        1 tctcaaggcc gagcgcgcgg taatccagcg aatgcagatt ggcgatgacc cgattgagct
       61 cgtcccagat ggccgcgcgc tcggccttcg acatcccggg cagcgactgg tcccacagca
      121 cgcggccctc gacatagtcc atgacgtaga acgcggtgcc gatcaccgcg tcgtcctcgc
      181 acagcacgtg cgggcgcgcc accgggaaac cggccttgtg cagagctgag atcacgcggt
      241 attcgcgctc gaccgcatgc gccgagggca agagcttgcc cggcggcttc cgccgcagca
      301 cgaaaccgcg tccgtcggct gaggcgagct tgtaggtggg attggactgg ccgcccttga
      361 actgctcgag cgtggcgatc gagaagccaa gaaatttttc gagccggcga acgtcgatgc
      421 gatggcgttc ctcgacgggc ttggtgccga tgaattgatc gaacatgagg ctctcacctg
      481 ccggcaaaag cttacctcag gagctattcc ctggagcgat gcgcagccag aacgtcaccg
      541 gcccgtcgtt gaccaggctc agttgcatgt ccgcgccgaa gcggccggtc tgcacacgcg
      601 gcgaccagtc atgcagcttc gcggcgaagc ggttgaagag cggctcggct tgcgcgggca
      661 gcgccgcgct cgaaaaactg gggcgcaggc cgctcgtggt gtcggcggca agggtgaact
      721 gcggcacggc gaggatctcg cccctcacgt ccttgagcga gagattcatc ttgcccgcgc
      781 cgtcggcgaa gatgcggtag ctcgccacgc gttcggcgag aagcagcgcc tgcacttccg
      841 tgtcggcccg ctcgacgccg acgagcgcca cgatgccggc gccgatcacg ccgacgggct
      901 cgccgcctac tctcacgttg gcgccgctga cgcgctggat cagcgcgatc aagcgatgat
      961 gtcggggatc aggcgcgcgc gcaggcgctg gatctggtcc ttgaggagga gcttgcgctt
     1021 cttcaggcgg cgcagctgca gctggtcctg cgtcgggtcg atgacgaggc ggccgatgac
     1081 gtcgtcgagg tcatggtgct cgacctcgag cgccatgagg cgctggcgga tttgggcttt
     1141 ttcctcttcg ctcggaggac tctcgatcac gccgtgaaat acctcatgcc gatggcgcgc
     1201 atcctatccc tatcctccgg gccgcgaaag ccctccccat gcgtcgcagc gaccatgacg
     1261 ccgagcctcg atgcctcgac tacctagtaa gtctttgaag gcctgaagta ctcgatgcgg
     1321 tcgatcgagt gcacggtcac gtcccgcacc cgcgccccct gcagcacctt gagcggcact
     1381 gcggtgcccg cggcaccttt tccccagacc ttgcggtaga agtcggcgag cgtcgcgacc
     1441 tcgtcggcac ccacggcgag gatgatgtcg ccgctcttga ggcccgcgcg agccgccggt
     1501 ccatcggcag aaacccgcgc gacgaagagc cgccccctcg cttcttcggc gttcacccca
     1561 agccagggcc gcgccgggcc gctgcgcttg ccattggcga tgagctcgtc gaggatcggc
     1621 ttcaggaggt cgatcggcac gaacatgttg cccggggact gcgtgcccgg cgcgccggca
     1681 tcgccgacga taagcgagcc tatgccgagc agctcgccct tcggcccgat cagtggcgcg
     1741 cccgaccagt ccatcaccgg cggataggtg tagatcgccg agtcgagcag gtactcccaa
     1801 cttcccgaga acggccggcg cgaaaccacg tacacgagcg tggggctgtc gcgcgcgctc
     1861 gccgtgaccg ccatcgccgg gtcgcgctcg gcgagggccg ccgagtcgcc aaggggtagc
     1921 ggcttcgccc ccatggcgcc cacgatcttg agaagcgcga accccgaagc gtggtcgtag
     1981 ccggcgagcg tcgccggcag cgtgcgtccg tcggcgccgg tcacctgcac cgattccgcc
     2041 tcgatcacaa ggtagccgat cgtgagcgca tagccgttgc gcacgagcac gcctgttcct
     2101 tgccgctgta ggccgagcgt ctcggcgctg cgcgcgttcg cctggatctt cgcgctcacg
     2161 ccgacgacgg cattgagcgt ttcgtcgggc ttgtcttgcg cgagtgcaat cgatgcctga
     2221 aagagcacca tgaagaagaa tcgacgcatg agccctcctt gttcactcga gaatgcgctc
     2281 cgcgcgcacg agaatcgact gcggaatgct tacaccgatc tcgcgcgacg tccttagatt
     2341 gaccacaact tcgatgctcg gttgttcgat cggcatgtcg gccggctttg ccaccttgag
     2401 aatacgatcc acgaacgtcg ccgcgcaccg gtaagtcttc tgccagttcg gaccgtacgt
     2461 ggcgactgtt atccccgcgc gcgtaacgca tatggagctc caggtttcgc ccagcgacgt
     2521 accccacttc tcgcagccca tcgacgaacg cttcttccag cgacgcgatc gagtctggat
     2581 cgattggccc acaagcaagc gacttggaag cgcttcggta tctggccctt caaagccctt
     2641 ggccacgccg gcggcgaaga acctgtctac gcgtcgtcat cccgcactcc tctggccatc
     2701 atgcgcctct cgcagccgct cgtgcaatcg gcggaacgaa aagggctaat attcctgacc
     2761 ccatgttcct ggcgctgctc tgcggcacat tggctttagg cgctggtctg ctggccgcgg
     2821 cgggctacag cgagggaacg gtggtggcgg cgcgaaccgc ggcgctgacg atcggctggc
     2881 tcttgttggt cgccacgctg gtcggcatgc agggcgtgcg gcgccgcagg ccggcggagc
     2941 acgccaccac ggtggtggtg tttgtttccg gtatcggcgt gctttatctc tgctattttc
     3001 attgggccca actgccacag cccgccgtcc ggctggccga cggtccgatc gtgcacgagc
     3061 cggcgctcga aaccgagctc gtcgccgagc cgcttgtggc aagtgcgatt gtcgtaagtc
     3121 cgcgggtagt cgcgcaggta acgtctgcgc cgattgtgga gaaaccgcgt atcgaaaccg
     3181 agccgatcgt cgaccgctgc tcttcgctcg gtggtgtgga ggccacgcag tgcagccgat
     3241 gcctaggcaa gtcgggcata gcccggatga tgtgcctgga aagcgcgcgg ctcgtatatt
     3301 gcgagggcag gccgaccgac gaggtgtgcc cctcgcccat tcccgcggcc acgagcgagt
     3361 acgtcgcgcc gggctagcgg cggccttgac cagctatcgc gtatggttag tccccggata
     3421 gcattcgaga tgtgcgcctg atcagctgga acgtgcaatg gtgccgcggc atggacgggc
     3481 gcgtcgaccc aaagcgcatc gccgccgagg cgaggcggct cgcggatccc gacgtgctct
     3541 gcctgcagga agtcgcgtgc aatttccccg agatggaagg cagcgcgggc gaggaccagg
     3601 tcctcgaatt gacccgcagc ctcgaagggt atgaaggatt cttcgcgccg gcggtcgata
     3661 ttcctggaaa gaaaggacgg cggcgcttcg gcaacctcgt gctcacgcgc ctcccggcgg
     3721 ggcgagtgat gcgccactcg ctgccgtggc ccgcctcacc gggcgtgccg agcatgccgc
     3781 gcgcagtggt cgaggtcgta gtcgactccg gccttggacc ggtgcgagta atgacgaccc
     3841 acctcgagta ctactcggct gaacaccgcg acgcccagat aggacgcctc ggggaaatcc
     3901 acctggaagc atgcgcggct cacctgcgcg tcgacgagcc cggcagcttc gagtcgcacg
     3961 agcggccaag cagcgcggtg ctctgcggtg acttcaatct caagcccgac gacccgctac
     4021 accgcaagat cctggacgcg ggcttcgccg acgcctggca ggcgctgcac cccggcaagc
     4081 ctcacccgca caccttcaag ctttacgaca agaacgagaa accgtattgc tgcgactatg
     4141 tgttcgtgac gcgcgagctc gcgccgcggc tgcgcgcgat ccgaatcgac tcccaaacgc
     4201 aggcatccga tcatcagccg gtgatggtgg aacttggctg agataagaaa gatcggagaa
     4261 gcgctcggcg ccgaagtgag cggcgtgcat ctctcgcagc cgatcgctgc cgagacgttc
     4321 gcgcgcatcc gcgcggcgtg gctcgaacac atggtgctgc gctttcgtgg ccagaagctc
     4381 accgattcgc agctcctcgc gttcagccgc cagttcggcg agctcgaccc gccgggaccc
     4441 aatccgctcg gcaggccctt cctgccggat caccctgaga tgaacgtcat atcgaacatc
     4501 aaggagggcg gcgtgccgat cggcgggctg ggggacggcg aagccatctg gcacgccgac
     4561 atgacctaca tcgagcgccc gcccatggcc gcgatcctgt acgcgatcga aattccgccg
     4621 gcgggcggcg acacgtattg ggcgaacatg gtgctcgcat acgaaacgct gcccgcgaag
     4681 ctgaaagcgc gcatcgaggg tcgcgaggcc gtgcacgacg cgacctacaa cagcgccggc
     4741 accctgcgca agggctacaa gcaggtcatc gatccgcgca ccgcgcctgg cgcgcgccat
     4801 cctctcgtgc gcatgcaccc ggagagcggg cgacggtgtc tctttcttgg ccggcggcgc
     4861 aacagctcta tcgtcggcct cgagctccat gagagcgagc gcctgctgga cgagctgtgg
     4921 cggcacgcca cgcaaccgca gttcacgatg cgccagcagt ggcgcgtggg cgacgtgatg
     4981 gtctgggaca accgctgcac gctccaccgg cgcgacggct tcgatcccgc ggcgcgccgg
     5041 ctcctgcacc gcacgcagat cagggcgtag acctggccgc gagcaggcgg cggatcttct
     5101 cgatacgcga gcggttcacc ccgaagtcgc ggcgcccgag gcgcgaagcc gagcgcacct
     5161 ggatcgccgc gccgtcgaag aagaactcca cgtcgtccac gaagcggagc agcctgctgc
     5221 ggaattcggc gtacagatag ttcgggtgcg cgcgcacgat gtgcgtgcgc ggcagcgcct
     5281 ccaccgccct tctcaggtct tccatgcttg ccttgagcgg cgcgatgtag tgctcgcggt
     5341 cggacggatc ggcctgcgag gagacgcagt tcggcgagcg ccggcacggg gcgagcaggc
     5401 cgtcctttac tcccaggttg gtcggccggc gccagtcgaa cactagaagt cctgcggctc
     5461 cggcgggaag cgcgggtcgc gcttttcgcg cagcgacgcg agcccttcct tcacctcggg
     5521 acccgtgaac ccgagcatct ccaggccgag cgacgcgtcg aacgccggcc ccgccatgcg
     5581 cagccagttg ttgagcgaat acttcgtcca gcgcagcgcg gtctgcgggc cggcggcgag
     5641 cttgcgcgcc acttcgagcg ccctgtcgcg caactcgctc tcctcgcagg aaagcgagac
     5701 gagtcccagg cgctcggctt cctcgccgct gatcgtgtcg caggtgagga ggtaatactt
     5761 cgctttcgcc atcccgcaaa gaagcggcca gacgagcgcc gcgtggtcgc ccgccgcgac
     5821 gcagaggcgg gtgtggccgt cgatcagcct ggcgctcttc gccgcaatcg agatgtcggc
     5881 catcaggccc gcggcgaggc ctgcgcctac cgccggcccg tggatcgccg agatgatgat
     5941 cttggagcag ttcagcatgt tgtagacgag gtcgcgcgcc tcgcgccaca cgcgcgcgcg
     6001 caccgcgaag tcgcgcgtca tctcttcgac catcgccatg tcgccgcccg cggagaaccc
     6061 tttgcccgcg ccgcgaagga gtgcgacgcg cgtctcggga tcgcgatcga catcgagcca
     6121 gacgcgcgcc atctcggcgt gcgcgcgcgc ggtcgcgacc gcgagcctgc cttcctcgcc
     6181 catcacgatc tcgaggatgc cggcctcggg ccggtggatc ttcaggtcct ggtatgcgcc
     6241 gtagtccatg ggctcattct ataatcgcgc accccatgct caggcgtctg ttcggcggct
     6301 cgcgggcgca ggacaactca cgcgagctcc ttgcgcaggc ctacgattcg ttcaaggcag
     6361 gcgcgtacga cgatgccgag cggctgtgcg cggcgtgcct cgacgcggct gcggatttcg
     6421 tggaggcgca tcatctccat gcgctgatag cgcttgcccg cggtgagctc gagccggcgt
     6481 tcacccgcct caagctcgct gccgggctcg acgattccgg ctctccgttc cacggcgccc
     6541 tcggaagcgc gctgcagtat ttcgaggcgc acgaggcagc agcccaccac ctcgcgcgag
     6601 cgctcgacct cgcgcggcgc gaagatcccg cgcgcgccga gctcatgcta tgcctttcgg
     6661 actcgctcat cgcacgcaag gagttccagc aggccgagcg cgtgctgcgc gagttgctga
     6721 gcgcgattcc ggagcacccc gaggcgctct cgcgcctcgc ctcggtgcgg ttttacgagt
     6781 ccgacgcgcg cgaggcgcgc agcctcatgg accgctatct ggcggcgcgg cccgaggcgg
     6841 gactacgcct gcggcgtgcg ctgatgatgc cggtcattct cgagtcgaac gaggagatcg
     6901 acgccttgcg cagccggttg gagcgcgacc tggacgagct gggcgaggcg cgccttgcgc
     6961 cgatccggga tccggtgtcg gaagtcggac tgacggcttt ctacgtcgcc taccatggcc
     7021 gccgcaaccg tgatctgctg cgcaagttcg gccgtgtctg tcgcgccctt taccccgcac
     7081 gcgccgaagc tccgaaacgc tcgccggccg gccgcaggcg cctgcgcatc gggttcgtgt
     7141 cgacgttctt ctttacgcat tcggtcggcc gcaccacgct cgggctcatc aaagatctgc
     7201 cgcgcagcga cttcgaggtc catgtctttg ccgtggcgcc ccacgaggac gagatggcgc
     7261 gggagatccg tctcgccgcc gagcgctacg cagcgctgcc gcgcaacctg gagcaggtac
     7321 gcgccgcgat cgaagcggcc gagctcgaca tccttctctt tgccgacatc ggcatggagc
     7381 cgctcacgac ttttctttcg ctctggcgcc tggcgccgct gcaactcaac acctggggtc
     7441 attcggtgac gagcggcatc gataccgtgg actactatgt ctccggcgaa agcgtcgagg
     7501 cagccggcgc gcaggagctc tacagcgaga agctgatgcg cctgcccggg tacttcatgc
     7561 cccgctacca ccgtccgccg gcgctgccgc gcaagtcgcg cgaggagctc ggattgcccg
     7621 ccgaccgtca tctctacttc tcgccgcaaa gcctgttcaa gctgcacccc gacttcgacg
     7681 ccgcgctgca cgggatactc gagcacgatc cgcgggccga gatcgtgctc agcgactcgc
     7741 gcgcgagctg gaaggagctg ctgcgcggcc gccttgcccg caggctgggc aaaaacgccg
     7801 accgcgtacg attcatgccc agcgtgccgc aacgcgaatt tcagcagtac ctggcagcgg
     7861 cagacgtcat catcgatccg ttccacttcg gcggatgcaa cacgagctgc gaggcgctcg
     7921 ggctcggcat cccgatcgtc acgctgccgg cgttccagct gccgggcagg ttcacgctcg
     7981 ggctttaccg cgagctcggc ctcgacgcct gcatcgcggg ctcggagagc gagttcgtcg
     8041 agctcgcggt gcgcgtgggc accgagcccg accttcgccg ctcgatctgc gcgcagatcg
     8101 ccgaacgcag cgagcgcctc ttcgagcgtc ccgataccgg gcgtgcgctg ggcgaggcgc
     8161 tccttcgcat cgcgcgcagg taagcatgct ggccttcgac gacatcgccc gcgcgcacga
     8221 gcgcatccgg ccgcaggtaa agcgcacgcc cgtgctcacc tcggctaccg tggatgcgct
     8281 caccggcgca gccgtcttct tcaagtgcga gaacttccag cgcatggggg ccttcaagtt
     8341 caggggcgcg tataacgcgc tttcccagct cgacaccggg agaaagaggc aaggggtagt
     8401 cgcgttctct tccggcaacc acgcgcaggc agtggcgctc gccggcaagc tcctcggcat
     8461 caagacggta atcgtgatgc cacaggacgc gccgcgggtg aagctcgaag cgactcgcgg
     8521 ctacggcgcc gaggtcgtgc agtacacgag gcacgaggat cgcgagacgc tcgccacgcg
     8581 gctcgcgcgc gaaagggggc tcacgctcgt gccgccgttc gaccacgagc acatcatcgc
     8641 cgggcagggc acggcgg
//
LOCUS       DASUNT010000064         5977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_797418, whole genome shotgun sequence.
ACCESSION   DASUNT010000064 DASUNT010000000
VERSION     DASUNT010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797418"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..624
                     /gene="mobB"
                     /locus_tag="VFX94_01320"
     CDS             133..624
                     /gene="mobB"
                     /locus_tag="VFX94_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEX5766848.1"
                     /translation="MKIFGFAGWSGSGKTTLIEQLIPRFVQRGLRVSLVKHAHHTFDV
                     DQPGKDSHRHRHAGASEILVTSSRRWVLMHELRGAHESPFEEQVKRISPCDLLLVEGF
                     KFAPIPKLEVWRAETGEALLHPNDPHIVAVASDAKVETKLPLLDLNDVDAIASFILRK
                     LEL"
     gene            621..1814
                     /gene="glp"
                     /locus_tag="VFX94_01325"
     CDS             621..1814
                     /gene="glp"
                     /locus_tag="VFX94_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX5766849.1"
                     /translation="MNQGLLSVDDALARLLAQAKPVADIEEVPTLEATNRVLARAQRS
                     TMDVPPMDNSAMDGFAVRTRDGDRLRVTQKIAAGSVGKPLEEGTAARIFTGAPIPPGA
                     DAIVMQEHTSTEGDRVVLSKKPSPGDWIRRVGSDVRKGGEILPAGKRLLPQDTGLAAS
                     VGIKTLPVHRKVRLGLFFTGDELVMPGEPLAPGRIYNSNRFTLRGLGEVFGCEVHDYG
                     IVPDSLEATRDALRRAAAQTDIIVTSGGVSVGEADYVKPAVEAEGKLLMWRIAMKPGR
                     PLAYGTVKTAAFIGLPGNPVSSFVTFLVFVRPFLLKTQGISQTAPRSVVARADFDWQE
                     PDARREFLRVKWNAQGGLELYPTQDSAVLTSTSWADGLVDNPAAHAIRKGDLVRFLPY
                     AELYR"
     gene            1811..2068
                     /gene="moaD"
                     /locus_tag="VFX94_01330"
     CDS             1811..2068
                     /gene="moaD"
                     /locus_tag="VFX94_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEX5766850.1"
                     /translation="MKVKVCFFANLRERLGTGAEVVEIPDSASTVAGLRLHLMQRGGA
                     WQDVLGDMKVVRVAVNHDMAAANAPLNPGDEVAFFPPVTGG"
     gene            2070..2525
                     /locus_tag="VFX94_01335"
     CDS             2070..2525
                     /locus_tag="VFX94_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX5766851.1"
                     /translation="MKVSVQAEDFDLSTEVIEISASPRTGAVASFVGQVREVPMTLEH
                     YPGMTESAIRKIVEEARSQWQILDYAVIHRYGDLQPGDQIVLVAIASSHRGDAFAACQ
                     FIMDYLKTQAPFWKKEHHPDGARWVDARDSDGEAAERWRGLKQQRALKP"
     gene            complement(2497..4314)
                     /gene="ftsH"
                     /locus_tag="VFX94_01340"
     CDS             complement(2497..4314)
                     /gene="ftsH"
                     /locus_tag="VFX94_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX5766852.1"
                     /translation="MEKRTQFNLGYLLFALVAIFLVQQWWQQAQTIEVVPYSEFEKLL
                     AQDKIAEVVVSDQRITGKLKAPENGKTVAVANLVPPEVAERLSKYGVQYTQAHESTFL
                     RDVLSWVIPALVFFGVWYWLIRRMASQGGGLGGGFMAIGKSRAKVYVEKQTGVNFGDV
                     AGVDEAKAELQEVVEFLKNPKKYGRLGARIPKGVLLVGPPGTGKTLLARAVAGEAGVA
                     FFSISGSEFVEMFVGVGAARVRDLFEQARQKAPAIIFIDELDALGRARGAFGVGGHDE
                     KEQTLNQLLVELDGFDPSTGLVLLAATNRPEILDPALLRAGRFDRQVLVDRPDKAGRI
                     QILRVHSRKVTLEKEVNLEQVAALTPGFTGADLANLINEAALLATRRGAEAVSMRDFN
                     EAVERLIAGLEKKNRVLNPMERRVVAHHELGHAMVAMALPGTDPVHKVSIIPRGVGAL
                     GYTIQRPIEDRFLMTREELENKMAVLLGGRAAEEVVFGRISTGAHDDLAKVTDIARSM
                     VMRYGMVKNLGQVAYEEEQGRFLLNPGFQKPREYSEETAREIDVAVREIVKAAYEKAL
                     GILSREKGALERWAQKLLEKETLIEAELGELRASMHAAA"
     gene            4406..>5977
                     /locus_tag="VFX94_01345"
     CDS             4406..>5977
                     /locus_tag="VFX94_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX5766853.1"
                     /translation="MRFDKFTTKLQEALAHAQSQALGQDNQYIEPQHLLAALLEDEGI
                     AGLVAKAGGNANALRKSLADSISRLAKVEGTPGEVHLSRELAQLLNVADREAQKRGDQ
                     YIASELFLLAAAGDKGETGRILKQSGVAKPALEKAIQEVRGGENVSSQQGEEQRQALA
                     KYTLDLTARARQGKLDPVIGRDDEIRRCIQILQRRTKNNPVLIGEPGVGKTAIVEGLA
                     QRIVNGEVPESLKSKRVLVLDMAALLAGAKYRGEFEERLKSVLKELAKDEGNTIVFID
                     ELHTMVGAGKAEGAIDAGNMLKPALARGELHCIGATTLDEYRKYIEKDAALERRFQKV
                     LVDEPSVEATIAILRGLQERYELHHGVEITDPAIVAAAELSHRYITDRFLPDKAIDLI
                     DEAAAKIKIEIDSKPEAMDKLDRRLIQLKIEREAVKRESDEASRRRLELIDGEIERLS
                     KQYADLEEIWKAEKLQVQGSQHVKEELERLKNEMEAAKRKGDWQKVSEIQYGKIPQLE
                     AQLKHAADKPGTAQQQ"
BASE COUNT         1081 a         1965 c         1990 g          941 t
ORIGIN      
        1 gattctcgct ccggccgtca tcgtcggcgg ctggctcggc gccgaatacg gcagccggcg
       61 gttcgctaat ccgatcgtaa ggcgggtgct cgccgtagtg ctcgcgctcg ccggtgcgaa
      121 aatgatcgcg gtatgaagat cttcggcttt gccggctggt cgggaagcgg caagaccacg
      181 ctcatcgagc agctcatccc gcgcttcgtg cagcgggggc tgcgagtgtc gctcgtcaaa
      241 cacgcgcacc acacgttcga cgtggatcag ccgggcaagg actctcaccg tcatcgccac
      301 gccggcgcct cggagatcct cgtcacctcg tcgcggcgct gggtgttgat gcacgagctg
      361 cgcggcgcgc acgagtcgcc cttcgaggag caggtgaagc gcatttcgcc gtgcgacctg
      421 ctgctcgtcg agggcttcaa gttcgcgccg atcccgaagc tcgaggtctg gcgcgccgag
      481 actggagagg cgctgcttca ccccaacgat ccgcacatcg tcgcggtggc gagcgatgcc
      541 aaggttgaaa ccaagctgcc tttgcttgat ctcaacgacg tggacgccat cgcgagcttc
      601 atcctcagga agctcgagct gtgaaccagg gacttctctc ggtcgacgat gcgctcgcgc
      661 gcctgctcgc gcaagccaaa ccggtcgcgg atatcgaaga ggtgcctacg ctcgaggcga
      721 cgaaccgtgt cctcgcccgc gcgcagcgtt cgacgatgga tgtcccgccg atggacaaca
      781 gcgcgatgga cgggtttgcc gtccgaaccc gcgacgggga ccgcttgcgc gtcacgcaga
      841 aaattgccgc cggctcggtc ggcaaacctt tggaggaagg tacagctgca aggatattta
      901 ccggtgcgcc gattccgccc ggagccgatg cgatcgtgat gcaggaacac acgagcaccg
      961 agggcgatcg tgttgtcttg agcaagaagc ccagccccgg cgattggatc agacgcgtcg
     1021 gaagcgacgt gagaaagggc ggcgagatcc tgcctgccgg caagcgcctg ctgccccaag
     1081 acacggggct tgccgcgtcc gtcggcatca agacgcttcc ggtgcaccga aaggtgcggc
     1141 tcggcctgtt cttcaccggc gacgagctcg tcatgccggg cgagccgctc gctccgggcc
     1201 ggatctacaa ctcgaatcgc ttcaccttga gggggctggg cgaggttttc ggctgcgagg
     1261 tacacgacta cggcatcgtg cccgacagcc tggaagcgac ccgcgatgcg ctgcgccgtg
     1321 ccgcggcgca gaccgacatc atcgtcactt ccggcggcgt gtccgtaggc gaagccgact
     1381 acgtgaagcc tgccgtggag gccgaaggga agctcttgat gtggcgcatc gcgatgaagc
     1441 cggggcgccc gctcgcatat ggcaccgtga agacagcggc ctttatcggc ctgccgggca
     1501 acccggtgtc gagcttcgtg accttcctcg tcttcgtgcg gccgttcctg ctgaagactc
     1561 aaggaataag tcagacagcg cctcgctcgg tcgtcgcgag ggccgacttc gactggcagg
     1621 agcccgacgc gcggcgcgaa ttcctgcgcg taaaatggaa cgcgcagggc gggctggagc
     1681 tctacccgac gcaggactcg gcggtgctga cgtccacctc ctgggcggac gggctggtcg
     1741 acaatcccgc ggcgcatgcg atccgcaagg gtgatctcgt gcgtttcctg ccttacgcgg
     1801 agctgtaccg gtgaaagtga aagtttgttt ttttgccaac ctgcgcgagc ggctcggcac
     1861 aggggcggag gtcgtcgaga ttccggattc ggcgagcacc gtcgcgggcc tgcggctgca
     1921 cctcatgcag cgcggagggg cctggcagga tgtgctcggc gacatgaaag tcgtccgcgt
     1981 cgcggtcaac cacgacatgg cggcggcgaa tgcgccgctg aacccgggcg acgaggtggc
     2041 cttcttccct ccggtcaccg gcggctgaca tgaaagtctc ggtacaggcc gaggatttcg
     2101 acctcagcac ggaagtcatc gagatctcgg ccagtccgag gaccggggcg gtggcgagct
     2161 tcgtcggcca ggtgcgcgag gtgccgatga cgctagagca ttacccgggc atgacggaga
     2221 gcgcgatccg caagatcgtc gaagaggcgc gctcgcaatg gcagatcctc gactacgccg
     2281 tgattcaccg ctacggcgac ctgcagcccg gcgaccagat cgtgctcgtc gcgatcgcga
     2341 gctcgcaccg cggcgacgcg ttcgccgcct gtcagttcat catggattac ctgaagaccc
     2401 aggcgccgtt ctggaagaag gagcaccatc ccgacggcgc gcgctgggtc gacgcccgcg
     2461 actccgacgg cgaggccgcc gagcgctggc gcgggctcaa gcagcagcgt gcattgaagc
     2521 cctgagctcg ccgagctccg cttcgatcag cgtctccttc tcgagcagct tctgcgccca
     2581 gcgctcgagc gctccttttt cccgcgacag gatgccgagc gccttctcgt aggccgcctt
     2641 cacgatctcg cgcaccgcga cgtcgatctc gcgcgcggtc tcctcgctgt attcgcgcgg
     2701 cttctggaat ccgggattga gcagaaaccg gccctgctcc tcctcgtacg ccacctgccc
     2761 gaggttcttc accatgccat agcgcatcac catgctgcgc gcgatgtcgg tcaccttggc
     2821 gaggtcgtcg tgcgctccgg tcgagatgcg gccgaacacg acctcctcgg cggcgcggcc
     2881 gccgagaagc accgccatct tgttctcgag ctcctcgcgc gtcatgagga agcggtcctc
     2941 gatcggacgc tggatggtgt aaccgagcgc gccgaccccg cgcgggatga tcgatacctt
     3001 gtgcacgggg tcggtgccgg gcagcgccat cgcgaccatt gcatggccga gctcgtggtg
     3061 tgcgacgacg cggcgctcca tcggattgag gacgcggttc ttcttctcga ggccggcaat
     3121 gaggcgctct accgcctcgt tgaaatcgcg catcgacacc gcctcggcgc cgcgccgggt
     3181 ggcgagaagc gccgcctcgt tgatcaggtt ggcgaggtcg gcgccggtaa agccgggcgt
     3241 gagcgccgcg acctgctcga ggttcacctc cttctcgagc gtcactttcc tggaatgcac
     3301 ccgcaggatc tggatgcgcc ccgccttgtc ggggcggtcg accagcacct ggcggtcgaa
     3361 gcgcccggcg cgcagcagcg cggggtcgag gatctcggga cggttcgtcg cggcgaggag
     3421 cacgaggccc gtggacgggt cgaacccgtc gagctcgacg aggagctggt tcagcgtctg
     3481 ctccttctcg tcgtggcccc ccacgccgaa ggcgccgcgt gcccggccga gtgcgtcgag
     3541 ctcgtcgatg aagatgatcg ctggcgcctt ctgccgcgcc tgctcgaaca ggtcgcgcac
     3601 gcgcgcggcg cccacgccga cgaacatctc gacgaactcc gagcccgaga tcgagaagaa
     3661 cgcgacgccc gcttcgcccg ccaccgcgcg cgcgagcagc gtcttgcccg ttcccggcgg
     3721 cccgaccagc agcacgccct tcgggatacg cgcgccgagg cgcccgtatt tcttcgggtt
     3781 cttgaggaac tccaccactt cctgcagctc ggccttcgcc tcgtcgacgc ccgctacgtc
     3841 gccgaagttg accccggtct gcttctcgac gtagaccttg gcgcggctct tgccgatcgc
     3901 catgaagccc ccgccgagcc cgccgccctg gctcgccatg cggcggatga gccagtacca
     3961 gacgccgaag aagacgagcg cggggatcac ccacgagagc acgtcgcgca ggaaagtgct
     4021 ttcatgcgcc tgcgtgtact ggacgccgta cttggaaagc cgctcggcga cctcgggcgg
     4081 caccaggttc gcgaccgcca ccgtcttgcc gttctcgggc gccttaagct tcccggtgat
     4141 gcgctggtcg gacaccacga cctcggcgat cttgtcctgt gccagcagct tttcgaattc
     4201 actgtagggc acgacctcga tggtctgcgc ctgctgccac cattgctgga ccaggaagat
     4261 ggcgacgagc gcgaagagca ggtaaccgag gttgaactgg gtgcgctttt ccatcgcctg
     4321 gagatggggg caaaaaccct gctttccaag caagaatgag gcttgaaagg cgccgcggga
     4381 tccccatttc tccgaaggga ctattatgag attcgacaaa ttcaccacca agctccagga
     4441 ggccctggcg catgcccaga gccaggcgct cggccaggac aaccagtaca tcgagccgca
     4501 gcacctgctc gcggcgctgc tcgaggacga aggcatcgcc ggcctggtcg ccaaggcggg
     4561 cggcaatgcc aacgccctca ggaaaagcct ggccgactcg atctccaggc tcgcaaaggt
     4621 cgagggcacg cctggagaag tacacctctc gcgcgagctc gcccagctcc tcaacgtcgc
     4681 cgacagggaa gcccagaagc gcggcgacca gtacatcgcc tccgagctct tcctgctggc
     4741 cgccgccggc gacaagggcg agaccgggag gatcctcaag caatccggtg tagcgaagcc
     4801 cgccctcgaa aaagcgatcc aggaagtgcg cggcggcgag aacgtttcct cgcagcaggg
     4861 agaggagcaa cggcaggcgc tcgccaaata cacgctcgat ctgacggcac gcgcaaggca
     4921 gggaaagcta gacccggtca tcggcagaga cgacgagatc cgccgctgta tccagatcct
     4981 gcagcggcgc acgaagaaca accccgtcct gatcggcgag cccggggtcg gcaagacggc
     5041 catcgtcgaa ggcctcgccc agcgcatcgt caacggcgaa gtcccggaga gcctgaagag
     5101 caagcgcgtg ctggtgctcg acatggcggc actgctcgcc ggcgcgaagt accgcggcga
     5161 gttcgaggag cggctgaagt cggtgctgaa ggagctggcg aaggacgaag gcaacaccat
     5221 tgtgttcatc gacgagctcc acaccatggt gggcgccggc aaggccgagg gcgcgatcga
     5281 cgcgggcaac atgctcaagc ccgcgctcgc gcgcggggag ctgcactgca tcggcgcgac
     5341 cacgctcgac gagtaccgca agtacatcga gaaggacgcg gcgctcgagc ggcgcttcca
     5401 gaaggtgctg gtcgacgagc cgagcgtgga ggcgacgatc gccatcctgc gcggcctgca
     5461 ggagcgctac gagctgcacc acggcgtcga gatcaccgac ccggcgatcg tggcggcggc
     5521 cgagctttcg caccgctaca tcaccgaccg tttcctgccc gacaaggcga tcgacctgat
     5581 cgacgaagcc gccgccaaga tcaagatcga gatcgactcc aagcccgagg cgatggacaa
     5641 gctcgaccgc cgtttgatcc agctcaagat cgagcgcgag gcggtgaagc gcgagagcga
     5701 cgaggcttcg cgcaggcgcc tggagctgat cgacggcgag atcgagcggc tgagcaagca
     5761 gtacgccgac ctggaagaga tctggaaggc ggagaaattg caggtgcagg gctcgcagca
     5821 cgtgaaggag gagctcgagc ggctgaagaa cgagatggaa gcggccaagc gcaagggcga
     5881 ctggcagaaa gtctccgaga tccagtacgg caagatcccg cagctcgagg cgcagctcaa
     5941 gcatgcggcg gacaagcccg gcacggcgca gcaacaa
//
LOCUS       DASUNT010000065         3679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_973114, whole genome shotgun sequence.
ACCESSION   DASUNT010000065 DASUNT010000000
VERSION     DASUNT010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973114"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VFX94_01350"
     CDS             <1..837
                     /locus_tag="VFX94_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX5766854.1"
                     /translation="NVVQKIVSAPFRALTAIFGGKQAGDPGNVAFVPGSAELSPPAEE
                     NVARVARGLAQRPGLGVEVHGAYDPARDLEALRMRAARRDIARAAGTEGSAELSDPAT
                     LRAAEKLYLERGGDRATLEALRKSEERYGRSLLQKLAATVNVEQAAAQALAHERADAV
                     RAALIEHGIDPARVRIGDAVEKQATKEGVETGLALAAGFASDAAAGASQPAPAPQAPD
                     DPVREAQRRLNAAGYEAGPVDGILGPRTKRALIHYQAVQGLKLTGELDAATHERLEAG
                     AL"
     gene            1234..1767
                     /locus_tag="VFX94_01355"
     CDS             1234..1767
                     /locus_tag="VFX94_01355"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX5766855.1"
                     /translation="MESSLLGRAIRAIDRFAELCGVVVAWLMVPLVAAVVYEVVARYA
                     FAAPTIWSFDLTYMLYGAMFMLGAAYALRVGAHIRTDFFYERWSRRTRGVIDSIAYVV
                     FFFPGIALFLWVGWDEAWYAYDIGELSEQTPWRPVLWPLKACVPLAAALLLLQGVSEL
                     TKSLHAALTGRAFAEGA"
     gene            1764..3191
                     /locus_tag="VFX94_01360"
     CDS             1764..3191
                     /locus_tag="VFX94_01360"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX5766856.1"
                     /translation="MSYETAIGLTMLAVMIAAIFLGFPISFTLLFLAFAFGWWGLGDV
                     VFNLAYFQTVGLMKESLLAAVPLFIFMGFVAEQAGLMERLFAALRLLLAPVRGSLFIV
                     VIVTAAAFAMATGIVGAAVTVLGIMAAPIMMRSGYDGQLAAGAITAGGTLGILIPPSV
                     MLIVMGPVLGVSVADLYAAAIVPGFLLAGLYVVYVTVRSLINPKLGPVLPKELRAPSV
                     WSALGAFFVGVVPLAGLVAATLGSILAGLATPTEAAGMGAAGALLLAAVYGKLTWQNF
                     QRALLQSLATGSMVLVLALTSNIFGAVFARLGAADWITDTLLSLPLPPGLMLALVTVL
                     IFLLGWPFEWPAVILVFLPIFYPVVNGLGVDLVWFGALVAVTLQTAYLSPPVAMSAYY
                     LRQVAKGWSLLTIYKGMFQFMGIQIVAIALIFLFPAIATWLPDYLFRSEALVVSEPPT
                     GLPPSQGLGESYDEMYQRSLRSLER"
     gene            complement(3198..>3679)
                     /locus_tag="VFX94_01365"
     CDS             complement(3198..>3679)
                     /locus_tag="VFX94_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5766857.1"
                     /translation="IHSAVHKGRPEVGCVIHTHSIAGMAISALDCGLLPITQTAMYFD
                     GVGMHEYESVAIDLDEQKRLVRDLGNYKAMILRNHGLLAVGPTVAEAFTNLFWLERAC
                     KAQVAAMSCNTKLHQPAQDVIAKTNHLYRPETRRRWGPLEWPALLRLADRKYPGFRD"
BASE COUNT          491 a         1242 c         1271 g          675 t
ORIGIN      
        1 aacgtcgtcc agaagatcgt gagcgcgccg tttcgcgcgc taacggcgat tttcggcggc
       61 aagcaggcgg gcgatcccgg caacgtggcg ttcgtgccgg gctcggccga gctttcgccg
      121 cccgcggaag aaaacgtggc gcgcgtcgcg cgggggctcg cgcagcgtcc gggcctcggg
      181 gtcgaggtgc acggcgccta cgaccctgcg cgcgacctcg aagcgctgcg catgcgcgcc
      241 gcccgccgcg acatcgcgcg cgcagcgggc accgaaggct cggccgagct gtccgacccc
      301 gcgacattgc gcgccgccga gaagctgtat ttggagcgcg gcggcgaccg ggcgacgctc
      361 gaggcgctgc gcaagagcga ggagcgctat gggcgaagcc tgctgcagaa gctcgcggcc
      421 accgtgaacg tcgagcaagc tgccgcgcag gcgctggcgc acgagcgcgc ggacgccgtg
      481 cgcgcagcgc tgatcgagca cggcatcgac ccggcgcgcg tgcgcatcgg cgatgcggtc
      541 gaaaagcagg ccacgaagga aggggtggag accgggctcg ccctcgccgc gggcttcgcg
      601 tccgacgcgg ccgcgggcgc ctcgcaaccc gcgcccgcgc cgcaagcgcc cgatgatccc
      661 gtgcgcgaag cgcagcggcg cctgaacgcc gccggctacg aagccgggcc cgtcgacggt
      721 atcctcggcc cgcgcaccaa gcgcgcgctc atccactacc aggcggtgca ggggctcaag
      781 ctcaccggcg agctcgacgc cgcgacgcac gagcggctcg aagcaggtgc tctataagca
      841 ttatcgccag aagtctggtc tccttcgttt acgacttcgt atgccgctgc tcaatgcgga
      901 gggcgagaat ggcagcgacc atcgcgccga cggcgttcgc cgatgaggtc tgtcatgctg
      961 tcggtcttgc cgcggatcgt gctcgccccc gtgcaaggta agcgcagccg cggtcacggc
     1021 gtccccgcgc cgggcgggcg gcgacgagcg ctgacccaaa cgggggcggg cagcggtgcg
     1081 gcggtccatc cgaaaggaaa ctctcgtttc cccaaccagg agacgccact cgatgtctgc
     1141 gccgcacggc gcccgcccag cagcagcttg cggcccgaat ccttaacgcg cgcttaaagc
     1201 tgccttaagt ctgccgcttc tacgctcgcc gccatggagt cctcgctcct cgggcgggcg
     1261 atacgggcga tcgaccgctt tgccgagctc tgcggcgtcg tggtggcctg gctgatggtg
     1321 ccgctggtcg cggccgtggt ctacgaggtg gtcgcgcgct atgccttcgc cgcgcccacg
     1381 atctggtcgt tcgacctcac gtacatgctg tacggggcga tgttcatgct gggtgcggcg
     1441 tacgcgctgc gcgtcggggc gcatattcgc accgacttct tctacgagcg ctggtcccgg
     1501 cgcacccggg gcgtgatcga ctcgatcgcc tatgtcgtgt tcttcttccc cggcatcgcg
     1561 ctcttcctct gggtgggctg ggacgaggcc tggtacgcct acgacatcgg cgagctctcc
     1621 gagcagacgc cgtggcggcc ggtgctctgg ccgctcaagg cgtgcgtgcc gctcgctgcg
     1681 gcgctgctgc tgctgcaagg ggtctccgag ctgacgaaga gcctgcacgc cgcgctcacg
     1741 ggccgcgctt tcgcggaagg cgcgtgagct acgaaaccgc gatcgggctg accatgctcg
     1801 ccgtgatgat cgcggcgatc ttcctcggct ttccgatctc gttcacgctg cttttcctcg
     1861 ctttcgcgtt cggctggtgg gggctgggcg acgtcgtgtt caacctggcg tacttccaga
     1921 ccgtcgggct catgaaggag tcgctgctcg ccgcggtgcc gctcttcatc ttcatgggct
     1981 ttgtcgccga gcaggcggga ctgatggagc ggctgttcgc cgcgctgcgg ctgctgctcg
     2041 cgccggtgcg cggctcgctc ttcatcgtcg tcatcgtcac tgccgcggcg ttcgccatgg
     2101 cgaccgggat cgtcggcgcc gcggtcacgg tgctcggcat catggccgcg ccgatcatga
     2161 tgcgctcggg ctacgacggc cagctcgccg cgggcgcgat caccgcgggg ggcacgctcg
     2221 ggatcctgat tccgccgtcg gtgatgctga ttgtcatggg gccggtgctc ggcgtctcgg
     2281 ttgccgacct ctatgccgcg gcgatcgttc ccgggttcct gctcgccggg ctctacgtcg
     2341 tctacgtcac ggtacgcagc ctcatcaacc cgaagctcgg tccggtgctg ccgaaggagc
     2401 tgcgcgcgcc gtcggtgtgg tctgcgctgg gcgctttctt cgtcggcgtg gtgccgctcg
     2461 ccggcctggt cgctgctacg ctcggatcga tcctcgccgg cctcgcgacg ccgaccgagg
     2521 cggcgggcat gggcgccgcc ggagcgcttc tgctcgctgc ggtatacggc aagctgacgt
     2581 ggcagaattt tcagcgggcg ctcttgcagt cgctggccac gggcagcatg gtcctcgtgc
     2641 tcgcgctcac gtccaacata ttcggcgcgg tgttcgcccg gctgggcgcc gccgactgga
     2701 tcacggacac gctgctctcg ctgccgctcc cgccggggct catgctggcc ctcgtcaccg
     2761 tgctcatctt cctgctcggc tggccgttcg agtggccggc ggtgatcctg gtcttcctgc
     2821 cgatcttcta tccggtggtc aacgggctcg gcgtcgacct ggtgtggttc ggggcgctgg
     2881 tcgccgttac gctccagacg gcataccttt ccccgccggt ggcgatgtcg gcctactacc
     2941 tgaggcaggt ggcgaaaggc tggtcgctgc tgacgatcta caaagggatg ttccagttca
     3001 tgggcatcca gatcgtcgcc atcgcgctga tctttctctt tccagccatc gcaacgtggc
     3061 tcccggatta tcttttccgc agcgaagcgc ttgtagtaag cgaaccgcct accggcctgc
     3121 ctccgtcaca gggcctcggc gagtcgtacg acgaaatgta tcagcgctcg cttcgcagcc
     3181 tggaaaggtg aagcctttca atcgcggaag cccgggtatt ttctgtctgc caggcgcaaa
     3241 agcgccggcc actcgagggg cccccagcgc cggcgcgttt ccggccggta gaggtggttg
     3301 gtcttcgcga tcacgtcctg cgccggctgg tgcagcttgg tgttgcacga catggcggcg
     3361 acctgcgcct tgcaggcgcg ctctagccag aaaaggttgg tgaaggcttc ggccacggtg
     3421 gggccgacgg cgagcaggcc gtggttgcgc aggatcattg ctttgtagtt gccaaggtcg
     3481 cgcaccaggc gcttttgctc gtcgagatcg atcgcgacgc tctcgtattc gtgcatcccg
     3541 acgccgtcga agtacatggc ggtctgcgtg atcggcagca ggccgcagtc gagcgccgag
     3601 atcgccatgc cggcgatcga gtgggtatgg ataacgcagc ccacctcggg ccgtcccttg
     3661 tgcacggcgc tgtgaatca
//
LOCUS       DASUNT010000066         4495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_622634, whole genome shotgun sequence.
ACCESSION   DASUNT010000066 DASUNT010000000
VERSION     DASUNT010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622634"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2715
                     /locus_tag="VFX94_01370"
     CDS             <1..2715
                     /locus_tag="VFX94_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5766858.1"
                     /translation="LNIKDRLARIDGVGQILQWGSGDYAMRVWLDPHKVAERNLSAND
                     IVRSIRQENVQAAAGVIGASPAPDRIDLQLTVNAQGRLQSEEEFADIIVKAEPGGAVT
                     RLRDVARVELGASEYALRSLLDNKPAAAIGVFAAPGSNALQISDRVRATMADIKKTMP
                     EGVDYEIVYDPTQFVRASIKAVIRTLLEATALVVLVVILFLQTWRASIIPLVAVPVSI
                     VGTFSLLYLFGFSINALSLFGLVLAIGIVVDDAIVVVENVERNIAEGRSPREATYQAM
                     KEVTNPIIAIALVLVAVFVPLAFISGLSGQFYKQFALTIAISTVISAINSLTLSPALA
                     ALLLKPHQEKKNWFFRGFNRYFGAAATAYSQSVGAVLSRKTLMMGIYVALLAVTGVLF
                     KAVPDGFVPQQDKQYLIGFAQLPDGATLDRTEDVMRRMGEIMMKQPGVENAVGFPGLS
                     INGFTNSSNSGIVFAALKSFDERKHPSLSGGAIAGQLNKQFAAIQDAFIVIFPPPPVQ
                     GLGTTGGFKLYLEDRASLGYEALDQATKAFLAKAYQTPELAGLFSTYQVNVPQLYADI
                     DRTKARQLGVPVTEVFETMQIYLGSLYVNDFNKFGRTYSVRVQADAPYRARAEDIGLL
                     KVRSNTGEMVPLSALMNVRPAAGPERAIRYNGFLAADISGRNAPGFSSGQAQDAIERI
                     AAETLPPGIGYEWTEITYQEILAGNSAIWIYPLAIFLVFLVLAALYESLALPLAIILI
                     VPLSLGAALTGVWLTHGDNNIFTQIGFMVLVGLAAKNAILIVEFARELEFAGRTPVQA
                     AIEASRLRLRPIIMTSIAFIMGVVPLAFSIGAGAEMRQAMGVAVFAGMIGVTAFGIFL
                     TPVFYVLVRAATRNRPLKHHGEIPHDAEPFGGGSVKAVPAAPHDNREV"
     gene            2717..2881
                     /locus_tag="VFX94_01375"
                     /pseudo
     CDS             2717..2881
                     /locus_tag="VFX94_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348677.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
     gene            2893..4086
                     /locus_tag="VFX94_01380"
     CDS             2893..4086
                     /locus_tag="VFX94_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5766859.1"
                     /translation="MIARHSPWTGLAALGIGNFVIGTGTLIVPGMLPLIAEGISVSLA
                     LAGQLVTAFAAAVCFGAPLLAGATSRYDRRSLLAATLLLFAVGHLAAALVSSFLPMLA
                     ARVVASAGAALFTAQAAATATLLVPPQARGRAIAFVFLGWSLAPVVGLPLGAYAGATW
                     GWRAGFALIAAGAALGAAAVWTLVPAGLRVKPVDGAMWRAIFTNPALVSAFAVSALLM
                     AAAFALFSYLVPALRAFVDASPETISLLLAAFGVMGIVGNLLAARFMDRLGAGNVVLF
                     GLLAMLAGHLLWPWSEGALVVLVAALLAWGLGGFASITAQQARLVSLSPALAPASIAL
                     NSSAVYLGQAVGSAAGGILIAHLPGSAGYASLSWLSVPMLLAAIGLAFLFSPTPTLAA
                     RRKTL"
     gene            4083..>4495
                     /locus_tag="VFX94_01385"
     CDS             4083..>4495
                     /locus_tag="VFX94_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HEX5766860.1"
                     /translation="MRSLALALLVLAGCATKLPEVEPQKLPPVPAEFKERWTIAAPAE
                     AQPRGEWWTAFNDPVLNELVERADRANATIQVAAARLAQARAVARITDADRALQASLG
                     ASARRARGIIGGDAGPARNLFATGVDLSYEVDLFGR"
BASE COUNT          629 a         1598 c         1469 g          799 t
ORIGIN      
        1 ctgaatatca aggaccgcct cgcgcgcatc gatggcgtgg gccagattct gcagtggggc
       61 agcggcgact acgcgatgcg cgtctggctc gacccgcaca aggtcgccga gcgcaacctc
      121 tccgccaacg acatcgtgcg ctccatccgc caagaaaacg tgcaggctgc tgccggcgtg
      181 atcggcgcct ctcccgctcc ggatcgcata gatctgcagc tcacggtcaa cgcccagggg
      241 aggctgcaga gcgaagagga gttcgctgac atcatcgtca aggccgagcc cgggggtgcg
      301 gtgacgcggc tgcgcgatgt ggcccgcgtc gagctgggcg cctccgagta cgcgctgcgc
      361 tcgctgctcg acaacaagcc cgcggcggcg atcggcgtct tcgccgcgcc gggctcgaac
      421 gcgctgcaga tttcggacag ggtgcgcgcc accatggccg atatcaagaa gaccatgccc
      481 gagggcgtgg actacgagat cgtctacgac ccgacgcagt tcgtgcgcgc ctcgatcaag
      541 gcggtgatcc gcacgctgct tgaggctacc gcgctggtgg tgctggtggt gatcctcttc
      601 ctgcagacgt ggcgcgcgtc gatcatcccg ctggtggcag tgccggtgtc gatcgtcggc
      661 acattctcgc tgctctacct cttcgggttt tcgatcaatg cattgagtct gtttggcctg
      721 gtgctcgcaa tcggtatcgt ggtggacgac gcgattgtcg tggtcgagaa cgtcgagcgc
      781 aacatcgccg aggggcgctc gccgcgcgag gcgacctacc aggcgatgaa ggaggtcacc
      841 aacccgatca tcgcgatcgc cctcgtcctg gtggcggtgt tcgtgccgct cgccttcatc
      901 agcggtttgt cgggccagtt ctacaagcag ttcgcgctca cgatcgcgat ttctaccgtg
      961 atctcggcga tcaactcgct caccctctca cccgcattgg cggcgttgct gctcaaaccc
     1021 catcaggaaa agaaaaactg gtttttccgc ggcttcaaca ggtatttcgg cgccgccgcg
     1081 accgcctaca gccaaagcgt cggcgctgtg ctttcgcgca agacgctcat gatgggcatc
     1141 tacgtggcgc tcctcgccgt gacgggagtg ctcttcaaag ccgtgccgga cggcttcgtg
     1201 ccgcagcagg acaagcagta cctgatcggc ttcgcgcagc tccccgacgg cgccacgctc
     1261 gaccgcaccg aggacgtaat gcggcgcatg ggcgagatca tgatgaagca gcccggtgtc
     1321 gaaaacgcgg tcggcttccc ggggctttcg atcaacggct tcaccaacag ctcgaattcg
     1381 gggatcgtgt tcgccgcgct caaatccttc gatgaaagga agcatccaag cctttccggc
     1441 ggcgcgatcg cgggacagct caacaagcag ttcgccgcga tccaggacgc cttcatcgtc
     1501 attttcccgc cgccgccggt gcagggcctc ggcacgaccg gcggcttcaa gctctacctg
     1561 gaagaccgcg cgtcgctcgg ctacgaggcg ctcgatcagg cgaccaaggc gttcctcgcc
     1621 aaggcgtacc agacgccgga gctcgcggga ctgttctcga cctaccaggt gaacgtgccg
     1681 cagctctacg ccgacatcga ccgcaccaag gcgcgccagc tcggcgtgcc ggtgacggaa
     1741 gtcttcgaga cgatgcagat ctatctgggc agcctgtacg tgaacgactt caacaagttc
     1801 ggccgcacct actcggtgcg cgtacaggcg gacgcaccct accgcgcgcg cgcggaagac
     1861 atcgggctgc tcaaggtgcg ctcgaacacg ggcgagatgg tgccgctctc cgcactcatg
     1921 aacgtgcggc cggcggccgg gcccgagcgc gcgatccgct acaacggctt tctcgccgcc
     1981 gacatcagcg ggcgcaatgc ccccggcttc tcgtcgggcc aggcgcagga cgcgatcgag
     2041 cgcatcgccg ccgagacgct gcccccgggc atcggctacg agtggaccga gatcacctac
     2101 caggagatcc tcgccggcaa ctcggcgatc tggatctacc cgctcgcaat cttcctcgtc
     2161 ttcctggtgc ttgccgcgct ctacgagagc ctcgcgctgc cgctcgcgat catcctcatc
     2221 gtgccgctct cgctcggcgc cgcgctcacc ggcgtctggc tcacccacgg cgacaacaac
     2281 atcttcacgc agatcggctt catggtgctg gtggggctcg cggcgaagaa cgcgatcctg
     2341 atcgtggagt tcgcgcgtga gctcgagttc gccgggcgca cgccggtgca ggccgccatc
     2401 gaggccagcc ggctgcgcct ccgaccgatc atcatgacct cgatcgcctt catcatgggc
     2461 gtggtgccgc tcgctttctc catcggcgcc ggcgcggaga tgcgccaggc gatgggcgtt
     2521 gcggtattcg ccggcatgat cggcgtcacc gccttcggca ttttcctcac gccggtgttc
     2581 tacgtcctcg ttcgcgccgc gacccgcaac cggccgctca agcaccacgg cgagatcccg
     2641 cacgatgccg agcctttcgg cgggggttcc gtaaaagccg tgccggccgc gccgcatgac
     2701 aaccgcgagg tttgaaatgg cctgggtctg gctcacgctc gccgggctgt tcgaagtcgt
     2761 gtgcgcgacc ggcatcaagt acacagatgc cttcacgcgc cttctcaaga ttttctaccg
     2821 ggaagccgga ccggcctccg cgccggtact cctcctcctg cacggcttcc cgacctcttc
     2881 gcggttcctg ccgtgattgc acgccactcg ccatggaccg ggctcgccgc gctcggcatc
     2941 ggcaacttcg tcatcggcac cggcacgctg atcgttcccg gcatgctgcc gctcattgca
     3001 gagggcatca gcgtgtcgct cgcgctcgcc ggccagctcg tcaccgcgtt cgccgccgcg
     3061 gtctgcttcg gcgcgcccct cctcgccggt gcgaccagcc gctacgaccg gcgcagcctg
     3121 ctcgcggcga cgctgctcct tttcgcggtc ggccacctcg ccgcggcgct cgtgtcctcg
     3181 ttcctgccga tgctcgccgc gcgcgtggtc gcctcggccg gggcggcgct ctttacggcg
     3241 caggctgctg cgaccgcgac gctcctcgtg ccaccgcagg cgcgtggccg cgccatcgcg
     3301 ttcgtgttcc tcggctggtc gctcgccccg gtggtcggct tgccgctcgg cgcgtatgcc
     3361 ggcgcgacct ggggatggcg cgcgggcttc gcgctgatcg cggccggcgc cgcgctcggc
     3421 gccgccgcgg tctggaccct cgtgccggca gggctgcggg tgaagccggt cgatggcgcg
     3481 atgtggcgtg cgattttcac caatcccgcg ctggtctcgg ccttcgcggt gagcgccctg
     3541 ctgatggcgg cggcgttcgc gctgttctcc tacctcgtgc cggcgctgcg ggcgttcgtc
     3601 gacgcctcgc ccgagacgat cagcctgctg ctcgccgcgt tcggcgtgat gggcattgtc
     3661 ggaaacttgc tcgcggcgcg cttcatggat cgcctggggg cgggcaacgt ggtgctgttc
     3721 gggctgctcg ccatgctggc gggccatctg ctctggccgt ggtccgaagg cgcgctcgta
     3781 gtgctggtcg ccgcgctgct cgcctggggg ctcggcggct tcgcgagcat caccgcgcag
     3841 caggcgcggc tcgtgtcgct ctcgcccgcg ctcgcgccgg cttcgatcgc gctcaactcc
     3901 tcggcggtgt atctcggcca ggccgtgggc agcgcggccg gcggaatcct gatcgcccac
     3961 ctgccgggca gcgccggtta cgcgtcgctt tcctggctca gcgttcccat gctactagcc
     4021 gccatcggcc tcgcgttcct gttttcaccg acgcccacgc tcgccgcgcg gaggaagacg
     4081 ttatgagaag ccttgcactc gccttgctgg tcctcgccgg ttgcgccacc aagctgcccg
     4141 aggtcgagcc gcagaagctt ccgcccgtcc cggccgagtt caaggagcgc tggaccatcg
     4201 ccgcgcccgc cgaggcgcag ccgcgcggcg aatggtggac ggcgttcaat gacccggtgc
     4261 tgaacgagct cgtcgagcgc gccgaccgcg ccaacgcgac gatccaggtg gcggcggcgc
     4321 gcctcgcgca ggcgcgcgcc gtggcgcgca tcaccgacgc cgaccgcgcg ctgcaggcct
     4381 cgctcggcgc gagcgcgagg cgcgcgcgcg gcatcatcgg cggcgacgcc gggccggcgc
     4441 gcaatctctt cgccacgggc gtcgacctct cctacgaggt ggatctcttc ggccg
//
LOCUS       DASUNT010000067         2733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1061201, whole genome shotgun sequence.
ACCESSION   DASUNT010000067 DASUNT010000000
VERSION     DASUNT010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061201"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VFX94_01390"
     CDS             <1..361
                     /locus_tag="VFX94_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766861.1"
                     /translation="YVQMDLEDKVQISGVTTTLIYLYVVPAKVAGKWSAQMPPAVSRQ
                     PATLSLKQQLTRVTGNVRLDGRDVALEDVKLRGDRLSFRLPGRQGEFSGQVKGNQIEG
                     IVDKGGTKLPWSASLGG"
     gene            358..648
                     /locus_tag="VFX94_01395"
     CDS             358..648
                     /locus_tag="VFX94_01395"
                     /inference="COORDINATES: protein motif:HMM:NF016050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like family protein"
                     /protein_id="HEX5766862.1"
                     /translation="MKLRISAKSAVFDLVLRDTPTPVSAKLEPDARQIVEPGTACFWT
                     EGDALALPFGRTPISTDERPKLASRCNVVGSIGNARDLAKIRAGDMVSVTRA"
     gene            651..860
                     /locus_tag="VFX94_01400"
     CDS             651..860
                     /locus_tag="VFX94_01400"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEX5766863.1"
                     /translation="MQRKRVNSSKLRSVGYDEPTRTLEVEMSNGQVFQYSGVYPEVYR
                     RFMAAPNPTSFFDDKIAEEYTAKRV"
     gene            886..1368
                     /locus_tag="VFX94_01405"
     CDS             886..1368
                     /locus_tag="VFX94_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766864.1"
                     /translation="MVRGLLLLLCVVASASSAATPQRTLPQPQAGFAFDSLYGIAAWL
                     RVQGREGYIGADVADAMGIARPQGEPLVAAWQRGFKSDDVLRIAQVSADDPLDFILFT
                     VQRPDDRVYFYLSTPLGGLQKAFLSIPSRNVVMPLERGEAELRWRAEVLYWKERSESR
                     "
     gene            1368..2472
                     /gene="prfB"
                     /locus_tag="VFX94_01410"
     CDS             join(1368..1448,1450..2472)
                     /gene="prfB"
                     /locus_tag="VFX94_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417196.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX5766865.1"
                     /translation="MEPERINQIASRLADLQARSAELRRYLDLEAKQKRLSEVETLLS
                     DAAVWSDPQRAQELGKEKKALEGVVGTLTRVGTGLADAAELFGLARAEGDDATLVGID
                     SDVQKIERDIAAMEFRRMFSNPLDPNNCFIDIQAGSGGTEAQDWAAMLERMYLKFCDR
                     RGFHAEVLEHTPGEVAGIKSATLKVSGDYAYGTLRTETGVHRLVRKSPFDANARRHTS
                     FASVFVYPEVDESIEVDVNPADLRIDTYRASGAGGQHVNRTDSAVRITHLPTNIVVQC
                     QNDRSQHRNRAEAMAMLKAKLYELETRRRQAEKQKLEEAKTDIGWGHQIRSYVLDQSR
                     IKDLRTGVEKGATQSVLDGDLDDFITESLKQGV"
     gene            2476..>2733
                     /locus_tag="VFX94_01415"
     CDS             2476..>2733
                     /locus_tag="VFX94_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX5766866.1"
                     /translation="MEEENKLVDERREKLASLRAAGQAFPNDFRPAHLAGELAQRFGQ
                     KTKQELEKTRVEVKIAGRMMLKRVMGKASFATLQDSSGRIQI"
BASE COUNT          531 a          884 c          917 g          401 t
ORIGIN      
        1 atacgtacag atggacctgg aggacaaggt ccagatcagc ggcgtcacca cgacgctcat
       61 ctacctttac gtagtgccgg caaaggtggc tggcaagtgg agcgcccaaa tgccgccggc
      121 agtcagccgc cagccggcga cgctgtcgct caagcagcag ctcacgcgcg tcacgggcaa
      181 cgtgcgcctg gacggcaggg acgtcgcgct cgaggacgtc aagctgcgcg gcgaccgcct
      241 gagcttcagg cttcccggcc gccagggcga gttcagcggg caggtgaagg gcaaccagat
      301 cgagggcatc gtcgacaagg gcgggacgaa gcttccctgg agcgcgagcc tcggcggatg
      361 aagctccgca tctcggcgaa gagcgccgta ttcgacttgg tgttgaggga caccccgacg
      421 ccggtcagcg ccaagctgga gccggacgcc agacagatcg tcgagcccgg caccgcgtgc
      481 ttttggaccg aaggcgatgc gctcgccctg cccttcggtc gcacgccgat ctccaccgac
      541 gagcggccca agctcgcaag ccgctgcaac gtggtcggaa gcatcgggaa tgcccgggac
      601 cttgcgaaga tccgcgcggg agacatggtc agcgtgacga gggcctgatc atgcagcgca
      661 agcgagtcaa ttcctccaag ctgcgctccg tcggctacga cgagcccacg cgcacgctgg
      721 aagttgaaat gtcgaacggg caggtcttcc agtacagcgg cgtctatccc gaggtctacc
      781 gtcgcttcat ggcggcgccg aacccgactt cgttcttcga cgacaagatc gcggaggaat
      841 acaccgcgaa gcgcgtgtag actacccgtg agggttaaaa cgaaaatggt tcgggggctg
      901 ctgctgttgc tttgtgttgt cgcgtcggca tcatcggccg cgacacccca gcgcacgctg
      961 ccccagccgc aggcgggctt cgcgttcgac agcctgtacg ggatcgctgc gtggctcagg
     1021 gtccagggcc gcgaaggcta tatcggcgcg gacgtcgccg acgccatggg catcgcgcgg
     1081 ccgcagggcg agccgctcgt cgccgcgtgg cagcgcggtt tcaaaagcga cgacgtgttg
     1141 cgcattgcgc aggtctccgc cgacgatcct ctggatttca tcctcttcac cgtgcagcgg
     1201 cccgacgacc gcgtgtactt ctacctctcg acgccgctag gcgggctgca gaaggcgttc
     1261 ctttccatcc cgagccgcaa cgtcgtgatg ccgctcgagc ggggggaggc ggaactgcgc
     1321 tggcgcgcgg aagtccttta ctggaaggag cgcagcgagt cccgctaatg gaaccggagc
     1381 gcatcaatca aatcgcctcc cgcctcgccg acctgcaggc ccggtcggcg gagctgcgga
     1441 ggtatctttg acctcgaagc caagcaaaag cggctgtcgg aagtcgaaac gctcctgagc
     1501 gacgccgcgg tctggagtga cccgcagcgc gcccaggagc tcggcaagga gaagaaggct
     1561 ctcgaaggcg tggtcggcac actgacccgc gtcgggaccg gcctcgccga cgcggcggag
     1621 ctcttcggcc tcgcccgcgc cgagggcgac gatgcgactc tcgtcgggat tgattcggac
     1681 gttcaaaaaa tcgagcgcga catcgccgcc atggagttcc ggcgcatgtt ctccaacccg
     1741 ctcgacccga acaactgctt catcgacatc caggcgggct cgggcggcac cgaggcgcag
     1801 gactgggcgg cgatgctcga gcgcatgtac ctcaagttct gcgaccggcg cggcttccac
     1861 gccgaggtgc tcgagcacac cccgggcgaa gtggccggca tcaagagcgc gacgctgaag
     1921 gtcagcggcg actatgcgta tggaaccctg cgcaccgaga cgggcgtgca tcgcctggta
     1981 cgcaagagcc cgttcgacgc caacgcccgt cggcacacct cgttcgccag cgtgttcgtc
     2041 tatccagagg tcgacgagtc gatcgaggtc gacgtgaacc ccgcggatct gcgcatcgat
     2101 acctatcgcg catccggcgc gggcggccag cacgtcaacc gcaccgacag cgcggtgcgg
     2161 atcacccacc tgcccacgaa catcgtggtg caatgccaga acgaccgctc tcagcaccgc
     2221 aaccgcgccg aagcgatggc gatgctgaag gcgaagctct acgagctcga gacgcgcagg
     2281 cgccaggccg agaagcagaa gctcgaggag gcgaagaccg acatcggctg gggacaccag
     2341 atccgctcgt acgtgctcga ccagtcgcgc atcaaggatc tgcgcaccgg ggtggagaaa
     2401 ggcgcgaccc agtccgtgct cgacggagac ctcgacgact tcatcaccga gagcctgaag
     2461 cagggggtgt aagggatgga agaagaaaac aagctggtgg acgagcggcg cgagaagctc
     2521 gcgtccctgc gcgccgccgg acaggcgttc cccaatgatt ttcggccggc gcacctagcc
     2581 ggcgagctcg cgcagcgctt cggccagaag acgaagcagg agctcgagaa gacgcgcgtc
     2641 gaagtgaaga tcgcgggccg catgatgctc aagcgggtga tggggaaggc gagcttcgcc
     2701 accctgcagg acagtagcgg ccgcatccag att
//
LOCUS       DASUNT010000068         2720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1236866, whole genome shotgun sequence.
ACCESSION   DASUNT010000068 DASUNT010000000
VERSION     DASUNT010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236866"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFX94_01420"
     CDS             complement(<1..242)
                     /locus_tag="VFX94_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX5766867.1"
                     /translation="MIKAVYPGTFDPLTRGHEDLVRRAATLFDTIVLAVADSKAKRPY
                     FSVEERIAMAREALADVKNVEVVGFSGLLTDFVREQR"
     gene            complement(211..783)
                     /gene="rsmD"
                     /locus_tag="VFX94_01425"
     CDS             complement(211..783)
                     /gene="rsmD"
                     /locus_tag="VFX94_01425"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX5766868.1"
                     /translation="MHSNKGTIRIIGGEYRGRRISVPDRRSLRPTPDRVRETLFNWLG
                     QSLQGLACLDLFAGSGALGFEAASRGAARVVLVEHDRLAFQALKKTLDLLKSPQVELV
                     CGDAFAYLGRSREAFDVVFLDPPFGQNVLPAVLRHLKTKRGMRVYLESSEPVAPAAPW
                     RELKRQRAGQVSYQLFEWRGHDQGGLPGNV"
     gene            805..1692
                     /gene="ftsY"
                     /locus_tag="VFX94_01430"
                     /pseudo
     CDS             805..1692
                     /gene="ftsY"
                     /locus_tag="VFX94_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003269901.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
     gene            1685..2332
                     /gene="ftsE"
                     /locus_tag="VFX94_01435"
     CDS             1685..2332
                     /gene="ftsE"
                     /locus_tag="VFX94_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX5766869.1"
                     /translation="MVSFSAVAKRYPGQQALYDVTFSLEAGELVFITGRSGAGKSTLL
                     KLIPAIERPSAGTVIVNGHNVGALKRAAIPYLRRNIGLVFQDQKLLYDRTVYDNVMLP
                     LAFSAHPPKEAARRARAALDKVGLAARERSNPVELSGGEQQRLAIARAVVNRPALLVA
                     DEPTANLDAESAARILDIFLAFNQVGVTVLLATHDQALVARYGRRVLHLEAGRMS"
     gene            2329..>2720
                     /locus_tag="VFX94_01440"
     CDS             2329..>2720
                     /locus_tag="VFX94_01440"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX5766870.1"
                     /translation="MTVWLRHHRQALSAAFKRLSLLNALVIGIALALPAGGYALLESL
                     RPASARLALEPRISLFLESQAPRADAEALGRRLRADPRISAVRFVPREEALKEMSAVQ
                     GLSEVIAALGRNPLPDAFVISSKVDIAA"
BASE COUNT          457 a          926 c          894 g          443 t
ORIGIN      
        1 cgctgctcgc gtacgaaatc ggtgagcagc ccggagaatc cgaccacctc gacgttcttc
       61 acgtcggcga gcgcctcgcg cgccatcgcg atgcgctcct cgacgctgaa gtagggtcgc
      121 ttcgccttgc tgtcggcgac cgccagcacg atcgtatcga atagcgtagc ggcacgacgc
      181 accaggtcct cgtggccgcg ggtgagcgga tcaaacgttc ccgggtagac cgccttgatc
      241 atggcctctc cattcgaaca gctggtagct cacctgcccg gcgcgctggc gcttgagctc
      301 gcgccacggc gcggctggcg ccacgggctc gctcgactcc aggtacaccc gcatcccgcg
      361 cttcgtcttg aggtgcctca ataccgcggg cagcacattc tgcccgaagg gcggatcgag
      421 gaaaaccaca tcgaacgctt cgcggctgcg accaaggtac gcaaaggcat cgccgcagac
      481 gagctcgact tgcggcgatt tcagcagatc cagggttttt ttcagtgcct ggaaagccag
      541 gcggtcgtgc tccaccagga cgacccgcgc cgcgccccgg gaagccgcct cgaagcccag
      601 cgcgccgctg ccggcgaaaa gatcgaggca tgcgagccct tggagcgatt ggccgagcca
      661 gttgaacaag gtctcgcgca cgcggtcggg cgtcggccgc aggctgcgcc ggtccggcac
      721 cgaaatccgc cggccgcgat attcgccgcc gatgatccgg attgtccctt tgttagaatg
      781 caccgaccca tggtatcgcc ctcttgggcc gcgcgcctca aggcaggcct cgcgcgcacg
      841 cgggatgtcc tcaacactcc cgtttcggaa ctcttcaccc ggcgcaagat cgacgaggcg
      901 ctttatgaag agctcgaagc cgcgctgctc caggctgact gcggcgtcgc cgccgcgcaa
      961 gcgctcatgg cggaattgag aaggaagaac gtcgcggacg gcgaagcgct gacgcgcgct
     1021 ttgaaagatg tgatcaccga gctgctttcg cccctggaga gaagactcga ggtcacaaaa
     1081 acgaggccgt tcgtgatcat gatcgcgggc gtgaacggct cgggcaagac cacctccatc
     1141 ggcaagcttg ccaggtggct tcagtcgcag ggcaagagcg tgctgctcgc ggcgggcgac
     1201 acgtttcgcg ccgcggcgcg cgagcagctt gtcgcctggg gcacgcgcaa cgccgtgccc
     1261 gtgatcgcgc aggagtcggg agacctggga gcggtggtgt tcgacgcgct ctccgcggcg
     1321 cgcgcgcgaa acgtggacgt ggtcatcgcc gacaccgccg ggaggctccc gacccagctg
     1381 caccttatgg aggaaatcaa gaaagtgaag cgtgtcgccg caaaggcgct cgagggcgct
     1441 ccgcacgagg tgttgctcgt gctcgacgcg aacaccggtc agaactcgct cgcgcaggtg
     1501 cgggccttcg atgcggcgct tggacttacc ggccttatcc tcaccaagct ggacggcacc
     1561 gcgaaagggg gcgtggtatg cgcgatcgcg aaagagaagc cggtgccgct gctctttgtc
     1621 ggcgtgggcg aaggtatcga cgatctgcgg cctttcgccg cgcgcgagtt cgccgaggcg
     1681 ctcgttggtt agcttctccg ccgtcgccaa gcgctatccc ggccagcagg cgctttacga
     1741 cgtgacgttt tcgctggagg caggagagct tgtcttcatt accgggcgct caggcgccgg
     1801 caaatcgacc ctcctcaagc tcatcccggc gatcgaacgg ccgagcgccg ggacggtgat
     1861 cgtgaatggc cacaacgtgg gcgcgctcaa gcgcgccgcc attccgtacc tcaggcgcaa
     1921 cattggcctt gtgttccagg accagaagct actctacgac cgtaccgtct atgacaacgt
     1981 gatgttgccg ctcgccttct ccgcgcatcc gccgaaggaa gcagcgcgcc gcgcgcgcgc
     2041 ggcgctcgac aaggtaggcc ttgcagcgcg cgaacgctcc aatccggtcg agctctcggg
     2101 cggcgagcag cagcgcctcg ccatcgcgcg cgcggtggtc aaccgcccgg cgctgctggt
     2161 cgctgacgag ccgacggcga acctcgacgc cgagtccgcg gcacgcatcc tcgacatctt
     2221 cctcgcgttc aaccaggtcg gcgtcacggt gttgctcgca acgcacgacc aggcgctggt
     2281 cgcgcgctac ggcaggcggg tgctgcatct cgaggcagga cgaatgtcat gacggtctgg
     2341 ctacgccacc atcgtcaggc cttgtccgcg gcgttcaagc ggctcagcct tctgaacgct
     2401 ctcgtgatcg gcatcgcgct cgctctgccc gcgggcggat acgccctgct cgagagcctc
     2461 cgcccggcga gcgcgcggct cgcgctcgag ccgcgcatct cgctgtttct cgagtcgcag
     2521 gcgccccgcg ccgacgccga agccctcgga cgccggctgc gcgcagaccc gcgcatcagc
     2581 gccgtgcggt tcgtgccgcg cgaggaagcg ctaaaggaaa tgagcgcggt acagggcctg
     2641 tcggaggtca tcgcggcgct cggccgcaac ccgctgcccg atgctttcgt catcagctcc
     2701 aaagtcgaca tcgcggcgga
//
LOCUS       DASUNT010000069         3651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_272403, whole genome shotgun sequence.
ACCESSION   DASUNT010000069 DASUNT010000000
VERSION     DASUNT010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272403"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFX94_01445"
     CDS             complement(<1..416)
                     /locus_tag="VFX94_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX5766871.1"
                     /translation="MKKAPQALFRLARAALERAGANSRMAEAAARHLVRAEAQGLPTH
                     GMSRVPFYCGMLRRGRADGAAQPAIVADRAAVCLIDNRDGLPYVSLDWAVEQVIQRAR
                     RNGIGFAGIRNSAHAGVMGIHLLPVAEAELVGLAFT"
     gene            435..1103
                     /locus_tag="VFX94_01450"
     CDS             435..1103
                     /locus_tag="VFX94_01450"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5766872.1"
                     /translation="MGKMIELTASDGHKLAAYRADPPGKPRGAIVVIQEIFGVNSHVK
                     SVADGFAADGYLAIAPAMFDRVQKNFDVGYTPPEIEKGRALRASITVDMAMKDAEAAV
                     KEAAKAGKVGIVGYCWGGFVAWMASAKIGGLSCAVPYYGGGILDNADIEPKVPVMGHF
                     GDKDQHIPVEGVKKLAEKHRKQQIFIYAADHGFNCDQRGSYNAPAAKQARSRTLEFFR
                     KHVG"
     gene            complement(1104..1661)
                     /locus_tag="VFX94_01455"
     CDS             complement(1104..1661)
                     /locus_tag="VFX94_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5766873.1"
                     /translation="MNRRAVVEKILSDRDSLLVIAGLGSTAWDCTAAGDSPLTFPMWG
                     AMGQAAMMGLGLELAQPRRRVLVITGDGEALMGLGALATIGAQKPENLTVVVIDNERF
                     GETGMQATHTAHGVDLAGVARSCGFKVLDLEHLPKAIHESPGPNFAAVKVAAEKIPLV
                     LPPREGALLKARFRRALLGPQADAE"
     gene            complement(1658..2176)
                     /locus_tag="VFX94_01460"
     CDS             complement(1658..2176)
                     /locus_tag="VFX94_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HEX5766874.1"
                     /translation="MWPDGIHRELGAAGVRMVGYVPDAGHKRLIELCQADASMRAVVL
                     TTEEEGIGLAAGAWLGGQRSTLLMQSSGVGNIVNVLGMVRECRFPLVTLVTMRGEQGE
                     FNPWQVPMGQAVRPVLESMGTVVHYVEKEDEVAPVVNAALRLAFGSYASVAVLISQRL
                     IGIKSFQEQAAK"
     gene            complement(2166..>3651)
                     /locus_tag="VFX94_01465"
     CDS             complement(2166..>3651)
                     /locus_tag="VFX94_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX5766875.1"
                     /translation="GAWLSDQELAWKLVVLAVERIRELENELGCFFDRNPDGTIHQKA
                     FAGQSFDRTVHKGDLTGIEIINRLAEQVWARRIGRLEEHRAVELIKASSGEALAGVLF
                     IDMRSGEYVFVRAKAVLLATGGGPTMYKYHTPSGDKSCDGLAMALRAGLALRDMEMVQ
                     FHPTGLLAGAHTRMTGTVLEEGLRGSGGYLLNGENERFMPRYDSKAERATRDVVSRAM
                     FAEMKKGNTTPNGGLYISMAHLGVSEVRKQFKGMVERCADCGFDLAGGLVEVVPTAHY
                     MMGGVEFAADCTTSLPGLFVAGEDSGGVHGANRLGGNGVANSTVFGAVAGDTMAAWVK
                     ARSHLDADEAAIGAAIARCDRPFDGRGASSLEPIREKLHEVMWEKVGIIRDAAGLLDA
                     QADLNALERALDGYSLPTAERAFNLTWHDWLNLKSLVAVSRAIAAAAIARRDSRGAHF
                     RADFPETGPLEQSAFTSIRNGIVAMKPVAFTRVAPGDTLLRHVA"
BASE COUNT          596 a         1270 c         1201 g          584 t
ORIGIN      
        1 ttcgtgaacg ctaggccgac gagttcggcc tcggcgacgg gcagcaggtg aatgcccatg
       61 acgccggcgt gagcgctgtt gcggatccca gcgaagccga tgccgttgcg ccgcgcgcgc
      121 tggatgacct gctcgacagc ccagtcgagc gacacgtaag gcagtccgtc gcggttgtcg
      181 atcaggcata ccgcggcgcg gtcggccacg atcgccggct gcgccgcgcc gtcggcgcgg
      241 ccgcgcctca gcatcccgca atagaacggc acgcgcgaca tcccgtgcgt cggcagcccc
      301 tgcgcttccg cgcgaaccaa gtggcgcgcc gccgcctcgg ccatcctaga attggcgccg
      361 gccctttcca gggcggcccg cgcgaggcga aacagcgctt gcggcgcctt tttcacagcg
      421 aggattctac ggccatggga aaaatgatcg agctcaccgc ctccgacggc cacaagcttg
      481 cggcctaccg cgcagatccc ccggggaaac cgcgcggcgc gatcgtcgtg atccaggaga
      541 tcttcggcgt caacagccac gtcaagtcgg tcgccgacgg cttcgccgcc gacggctacc
      601 tcgcgatcgc gccggcgatg ttcgaccgcg tgcagaagaa cttcgatgtc ggttacacgc
      661 cgcccgaaat cgagaagggt cgcgcgctgc gcgccagcat caccgtcgac atggcgatga
      721 aggatgccga agccgcggtg aaggaagccg ccaaggccgg caaggtgggc atcgtcggct
      781 actgctgggg cggcttcgtc gcctggatgg cgtcggcgaa gatcggcggc ctttcgtgcg
      841 ctgtccccta ttacgggggc ggcatcctgg acaacgcgga catcgagccg aaggtacccg
      901 tgatgggcca cttcggcgac aaggaccagc acatcccggt cgagggcgtc aagaagctcg
      961 ctgaaaagca cagaaagcag cagatcttca tctacgccgc cgaccacggc ttcaactgcg
     1021 accagcgcgg ctcctacaac gcacctgcgg ccaagcaagc gcgcagccgc acgctagagt
     1081 ttttcaggaa gcacgtcggc tgatcattcc gcgtcggcct gcgggccgag caatgcgcgg
     1141 cggaagcgcg ccttcagcag cgcgccctcg cgcggcggaa gcaccagcgg gattttctcc
     1201 gccgcgactt tcaccgcggc gaagttcggg ccggggctct cgtgaatggc tttcggcagg
     1261 tgctcgagat cgagcacctt gaagccgcag gagcgcgcga cgcccgcaag gtccacgccg
     1321 tgagcggtat gcgttgcctg catgccggtc tcgccgaagc gctcgttgtc gatgacgacc
     1381 acggtgaggt tctcgggttt ctgcgcgccg atggtggcga gcgcgcccaa acccatcagc
     1441 gcctcgccat cgccggtgat gacgaggacg cggcgccggg gctgcgcgag ctcgaggccg
     1501 aggcccatca tcgcggcctg gcccatggcg ccccacatcg ggaaggtgag cggcgagtcg
     1561 cccgcggcag tgcagtccca ggccgtcgat ccgaggccgg caatgacgag caagctatcc
     1621 cgatcggaga ggattttctc gaccaccgcg cgccggttca tttggccgcc tgctcctgaa
     1681 agctcttgat gccgatcagg cgctgcgaga tcagcacggc caccgaggcg tagcttccga
     1741 atgccagcct gagcgccgcg ttcacgaccg gcgcgacctc gtcttccttc tccacgtagt
     1801 gcacgaccgt gcccatcgac tcgagcaccg gacgcaccgc ctgtcccatc ggcacctgcc
     1861 aggggttgaa ctcaccctgc tcgccgcgca tcgtgaccag cgtgacgagc gggaagcggc
     1921 actcgcgcac catgccgagc acgttgacga tgttgccgac gccgctcgac tgcatgagaa
     1981 gcgtgcttct ctgtccgccc agccacgcgc ctgcagcgag gccgatgcct tcctcctcgg
     2041 tcgtcaggac gacggcgcgc atgctcgcgt ccgcctggca gagctcgatc aggcgcttgt
     2101 ggccggcgtc ggggacgtag ccgaccatgc gcacgcccgc ggcgccgagc tcgcgatgga
     2161 tgccgtcagg ccacatggcg cagcagcgta tcgccgggcg cgacgcgcgt gaaggcgacc
     2221 ggcttcatgg caacgatgcc gttgcggatg ctggtgaacg ccgactgctc gagcgggccc
     2281 gtttccggaa agtcggcgcg gaagtgcgcg ccgcgcgagt cgcgccgcgc gatcgccgcc
     2341 gcggcgatgg cgcggctcac tgcgacgagg ctcttgaggt tcagccagtc gtgccaggtg
     2401 aggttgaagg cgcgctcggc ggtgggcagc gaatagccgt cgagcgcgcg ctcgagcgca
     2461 ttcaggtcgg cctgcgcgtc gagcaggccg gcggcatccc ggatgatgcc gaccttttcc
     2521 cacatcacct cgtgaagctt ctcccggatg ggctccaggc tcgatgcccc ccggccgtcg
     2581 aaaggccgat cgcacctcgc aattgccgcg ccgatggctg cttcgtcggc atccaggtgg
     2641 ctgcgggctt tcacccaggc ggccatggta tcgccggcca ccgcgccgaa cacggtggaa
     2701 ttcgcgaccc cgtttccgcc caggcggttc gcgccgtgca cgccgccaga atcctctccc
     2761 gcgacgaaga ggccgggtag cgacgtggtg cagtcggccg cgaattcgac tccgcccatc
     2821 atgtaatgcg cggtcggcac gacttcgacc aggccgccgg ccaggtcaaa gccgcaatcg
     2881 gcgcacctct ccaccatgcc tttgaactgc ttcctgactt cgctcacccc gaggtgggcc
     2941 atcgagatgt agagcccgcc attaggggta gtgttgcctt tcttcatctc cgcgaacatg
     3001 gcgcgcgaca ctacatcgcg cgtcgcacgc tcggctttgg agtcgtagcg cggcatgaag
     3061 cgctcgttct ccccgttcag caggtagccg cccgagccgc gcagcccttc ttcgaggacc
     3121 gtgccggtca tgcgcgtgtg ggcgccggcg agcaaccccg tcggatggaa ctgcaccatc
     3181 tccatgtcgc gcagcgcgag tcccgcgcgc agcgccatcg cgagcccgtc gcagctcttg
     3241 tcgccggaag gcgtgtggta cttgtacatc gtcggcccgc cgccggtggc gaggagcacg
     3301 gccttcgccc gcacgaaaac gtattcgccc gagcgcatgt cgatgaagag cacgcccgcc
     3361 aacgcctcac cggaagacgc ctttatcaat tcgactgccc tgtgctcttc cagcctgccg
     3421 atgcggcgtg cccacacctg ctcggcgagc cggttgatga tctcgatgcc agtgaggtcg
     3481 cccttgtgca ccgtgcggtc gaagctctgg ccggcgaagg ccttctggtg gatcgtgcca
     3541 tcggggttgc gatcgaagaa gcagccgagc tcgttctcga gctcgcgaat cctttcgacg
     3601 gccagaacca cgagcttcca cgcgagctcc tggtccgaga gccacgcgcc g
//
LOCUS       DASUNT010000070         3499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_272503, whole genome shotgun sequence.
ACCESSION   DASUNT010000070 DASUNT010000000
VERSION     DASUNT010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272503"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VFX94_01470"
     CDS             complement(<1..1009)
                     /locus_tag="VFX94_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEX5766876.1"
                     /translation="MGKAITKGVIAAVLALTAPFAVNAAGLGRLTVLSPLGQPLNAEI
                     EIVALKPGEEDSLSARLAPLEAFEAAGIEPGVVLNSVRFAIERRDGRRIVRVTTLQPV
                     NEPFVELLVELQSQHGRLVREYTFLLDPAEYKARETYSATPTPKPMAPVQPAAPAQPA
                     APPPLVEPAKPASEAAPPPLAAAPAPAPSAEPEKPAAAEAEKPAAAVEEKPVAEGTKE
                     PAKEESEPLLADDASAYEVMKGDTLAKIAGDRLPPGITLNQMLVAIYRSNQDAFIREN
                     LNLVRAGRILNIPGSDDIGTVNMEEANRVVRAHMAEFRDYRARLAAAPAVADAASGQR
                     EA"
     gene            complement(1098..2219)
                     /gene="asd"
                     /locus_tag="VFX94_01475"
     CDS             complement(1098..2219)
                     /gene="asd"
                     /locus_tag="VFX94_01475"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX5766877.1"
                     /translation="MRVGIVGWRGMVGSVLVQRMCEERDFDHVEPVFFSTSQAGGSGP
                     AIGKPAEAVKDAKSVAELKKLPVILSCQGGDYTNEIYPRLRAEGWRGYWIDAASALRM
                     KDDALIILDPLNMPLIKKAISSGIKDYIGGNCTVSLMLMGMAGLFQRDEVQWMTSMTY
                     QAASGQGAAAMRDLVAQMGLIGSRARPLIDDPASAILDIDRVVTQSIRSRELPKDEIG
                     YPLAGSLLPWIDKDLGNGQSREEWKAQVEANKILGRNGNAIPMDGLCVRIGAMRCHSQ
                     AMTVKLRRPVPLDEVEGMLADAHDWVKVVPNRREESLAGLTPAAVSGKLTVPVGRLRK
                     LPMGEEYLAAFSVGDQLLWGAAEPLRRMVRILLDAGVKQ"
     gene            complement(2233..3285)
                     /gene="leuB"
                     /locus_tag="VFX94_01480"
     CDS             complement(2233..3285)
                     /gene="leuB"
                     /locus_tag="VFX94_01480"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX5766878.1"
                     /translation="MNIAVLPGDGIGPEIVAQALRVLEKLELPLTMKTAPVGGAGYEA
                     AGNPLPAETLSIARQADAVLFGAVGDPRYDSLERSKRPEQAILGLRKALGLFANLRPA
                     RVHPELAAASALKADVVSGLDILIVRELTGDIYFGEPKGIRTVAGVREGFDTMRYSEP
                     EIRRIARVAFEAARKRSKRVCSVDKANVLETSQLWREVVSQESGNYPEIELTHMYVDN
                     CAMQLVRNPKQFDVIVTGNMFGDILSDEASMLTGSIGMLPSASLDERGKGLYEPIHGT
                     APDIAGKEVANPLATILSAAMMLRYSLGQPAAAERIERAVSSVLQAGLRTADIHTAGR
                     RRVGTREMGDAVLAAL"
     gene            complement(3287..>3499)
                     /locus_tag="VFX94_01485"
     CDS             complement(3287..>3499)
                     /locus_tag="VFX94_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX5766879.1"
                     /translation="AAFPGFRLVVDLERQTVSPPDGQKTCRFDIEPFRKYCLLNGLDE
                     IGLTLRHADKIRAFEAQRKAQYPWYF"
BASE COUNT          549 a         1164 c         1139 g          647 t
ORIGIN      
        1 ccgcttcgcg ctggcccgaa gccgcgtctg ccactgccgg ggcggcggcg agccgggcgc
       61 gatagtcccg gaactcggcc atgtgcgcgc gcacgacgcg attcgcctct tccatattca
      121 cggtgccgat gtcgtcggag cccgggatgt tgaggatgcg gccggcgcgc acgaggttca
      181 ggttctcgcg aatgaaggcg tcctggttag aacgatagat ggcgaccagc atctggttga
      241 gcgtgatccc cggcggcagc cggtcgcccg cgatcttggc gagcgtatcg cccttcatga
      301 cctcgtaggc gctcgcatcg tctgcgagaa gcggctctga ttcttccttt gccggttcct
      361 tcgtcccttc ggctacaggc ttttcttcaa ccgcggcggc aggcttctcg gcttcggctg
      421 cagccggctt ttcgggttcc gccgacggcg ccggcgcagg cgctgcggca agcggcggcg
      481 gcgcggcttc ggatgcgggc ttcgccggct cgaccagtgg aggcggtgcg gcaggctgcg
      541 cgggagcggc cggctgcacc ggagccatcg gctttggtgt cggcgtcgcg gagtacgttt
      601 cgcgcgcctt gtactcagcc gggtcgagca ggaaagtata ttcacgaacc aggcgcccgt
      661 gctgcgattg caactcgacg agaagttcga cgaacggctc gttgaccggc tgcaatgtcg
      721 tgacgcgcac gattcgcctg ccgtcgcgcc gctcgatcgc gaagcgcacg ctgttcagca
      781 cgacgccagg ctcgatgccc gcggcctcga acgcttcgag cggcgcgagg cgagcagaga
      841 gcgaatcttc ctctcccggc ttgagcgcga cgatttcgat ctctgcgttg agcggctgcc
      901 cgagcggcga cagcacggtg agcctgccta ggccggcggc gttcacggcg aatggcgccg
      961 tgagcgccag tacggcggcg attacccctt tagttattgc tttacccact ggctgcccgt
     1021 ccccgatgtt cttagcagga tcaactacct taacatcatg ggcttagggc tgcaaatcga
     1081 gaaactgtct tacgtctcta ctgcttcacc ccggcgtcga gaaggatgcg caccattcgc
     1141 cgcagaggct cggctgcccc ccacaagagc tggtctccga ccgaaaaagc tgccaaatat
     1201 tcctcaccca tgggcagttt tcgcaggcgg cccaccggaa cggtcagctt tccgctcacg
     1261 gcggccgggg tcagcccggc aagtgactcc tcccggcggt tcgggacgac cttcacccag
     1321 tcgtgggcgt cggcaagcat gccctctacc tcgtccagcg gcacgggacg gcgcagcttc
     1381 acggtcatcg cctggctgtg gcagcgcatt gccccgatgc gcacgcagag gccatccatc
     1441 gggatcgcgt ttccgttccg gccgagaatc ttgttcgcct cgacctgcgc cttccattcc
     1501 tcgcggctct ggccgttgcc gaggtccttg tcgatccacg gcaagaggct gccggcgagc
     1561 ggatagccga tctcgtcctt gggcagctcg cgagagcgga tcgattgcgt cacgacgcga
     1621 tcgatgtcca ggatggcgga cgccggatcg tcgatcagcg ggcgggcgcg gctgccgatc
     1681 aggcccatct gcgccacgag gtcgcgcatc gcggcagccc cctgtccgga ggccgcctgg
     1741 tacgtcatgc ttgtcatcca ctgcacttcg tcgcgctgga aaagccccgc catgcccatc
     1801 agcatcaggc tcacggtgca gttaccgccg atgtaatcct tgatgccgga cgaaatcgct
     1861 ttcttgatga gcggcatgtt caatggatcg agaatgatga gcgcgtcgtc cttcatccgc
     1921 agcgcgctcg cggcgtcgat ccagtagccg cgccagcctt cggcgcgaag cctcggataa
     1981 atctcgttgg tgtagtcccc gccctggcac gagaggatga cgggcagctt cttcagctcc
     2041 gccacgcttt tcgcgtcctt caccgcctcg gccggcttgc cgatcgccgg gccgctgccc
     2101 ccggcctgcg aggtggagaa gaacaccggc tcgacatggt cgaagtcgcg ctcctcgcac
     2161 atgcgctgca cgagcaccga gcccaccatt ccgcgccagc cgacgattcc gactctcaac
     2221 ataggccgta gtctataggg cggcgaggac ggcgtcgccc atctctctgg ttccgacgcg
     2281 gcgccttccg gccgtgtgga tgtcggcggt gcgcaggccc gcctgcagca cgctcgacac
     2341 ggctctttcg atgcgctctg ccgcggccgg ctggccaagc gaatagcgca gcatcattgc
     2401 cgccgacagg atcgtggcga gcggattcgc cacctctttg cccgcgatgt cgggcgcggt
     2461 gccgtgaatc ggctcgtaaa gccctttgcc gcgctcgtcg agcgaggcgg agggcagcat
     2521 gccgatcgag ccggtgagca tggaagcctc gtccgaaagg atgtcgccga acatgttgcc
     2581 ggtgacgatg acgtcgaact gcttcgggtt gcgcacgagc tgcatcgcgc agttgtccac
     2641 gtacatgtgg gtcagttcta tttccggata attgccggat tcctgagaaa caacttccct
     2701 ccacagctgc gaggtctcca gcacgttcgc cttgtcgacc gagcaaacgc gcttcgagcg
     2761 cttgcgcgcc gcctcgaacg cgacgcgcgc gatgcgccgg atttcgggct cgctgtagcg
     2821 catggtgtcg aagccttcgc gcactccggc caccgttcgg attcctttcg gctcgccgaa
     2881 gtagatgtcc ccggtcagct cgcgcacgat gaggatgtcg agcccggaga cgacatccgc
     2941 cttcagcgcc gaagcggcag cgagctcggg gtggacccgc gccgggcgca ggttggcgaa
     3001 aaggccgagc gccttgcgca gcccgaggat cgcctgctcg gggcgcttgg agcgctcgag
     3061 gctgtcgtag cgcgggtcgc cgacagcgcc gaagagcact gcgtcggcct gcctggcgat
     3121 gctcagtgtt tctgccggga gcgggttccc ggccgcctcg taacccgcgc cgccgacggg
     3181 cgcggtcttc atcgtgagag gcagctcgag cttctccagg acccgcagcg cttgcgcaac
     3241 gatctccggc ccgatgccgt caccgggaag aacggcgatg ttcatcctag aaataccagg
     3301 ggtactgcgc cttgcgttgc gcttcgaacg cgcggatctt gtccgcgtgg cgcaacgtga
     3361 ggccaatctc gtccagcccg ttcagaagac agtacttgcg gaacggctcg atgtcgaagc
     3421 ggcaggtctt ctggccgtcc gggggggaaa ccgtctgccg ctcgagatcg acgacgaggc
     3481 gaaatcccgg gaacgccgc
//
LOCUS       DASUNT010000071         2805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1062106, whole genome shotgun sequence.
ACCESSION   DASUNT010000071 DASUNT010000000
VERSION     DASUNT010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062106"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VFX94_01490"
     CDS             complement(<1..447)
                     /locus_tag="VFX94_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX5766880.1"
                     /translation="MTWSIIARDESGAFGVAVATRFFAVGALCPHAESSVGALCTQAL
                     VNPYYGRHGLDLLRAGISAPDVVKMLTSPDEGRDHRQLHVVDDEGRIGQHTGAKCVDW
                     CGAVAGGAFSVAGNMLAGPQVVEQTARAFKASKKPFAERLIEAMEAG"
     gene            complement(483..944)
                     /locus_tag="VFX94_01495"
     CDS             complement(483..944)
                     /locus_tag="VFX94_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5766881.1"
                     /translation="MRILVPVLDSVNALPAVRYVVREFMGRERPEVQLLCVRSGLALA
                     FVPGLAEKSVASARELLKWHGVRHTVKLQAGDKAKAILAAAVDTSPDLIVLGTARYRS
                     ATRMSEQAVVHSLLETSPAPVVVVAGKEVSSLERYGIAAGLGATLGLILFA"
     gene            complement(947..1447)
                     /locus_tag="VFX94_01500"
     CDS             complement(947..1447)
                     /locus_tag="VFX94_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5766882.1"
                     /translation="MKILVPVDASQAALAPLSYLESLERSGVPLEVLLVNVQPRFHRH
                     VAQFTSRAARDALRAERSRASMARAIEALALKNIPFRAFAEIGKPAERIAAIAERERV
                     DEVMMGVGRHPAWLRWVNPSIAQAVMEQTDIPVTVLARGRAGVFERYALPAGVAGLAA
                     LLLAGE"
     gene            complement(1444..2139)
                     /locus_tag="VFX94_01505"
     CDS             complement(1444..2139)
                     /locus_tag="VFX94_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX5766883.1"
                     /translation="MEIFLAPEFWSALAAIIVIDLVLAGDNAIVIALAARNLPRTLQR
                     RAVIWGTVGAVAVRASLTVVVLWLLGIPGLMLAGGILLAWIAYRLLAGDESGRDHDVA
                     PATSFWAAMRTIVIADAVMGLDNVLAVAGAAHGSVLLVVAGLLISIPIVVWGSTLILH
                     WIERFPALLYLGGAVLAWTAAKMITDEPLYQEWIDARPGIRSAIYLVIVGGVLAVPYL
                     RNRKAARLKEVMQ"
     gene            2375..>2805
                     /locus_tag="VFX94_01510"
     CDS             2375..>2805
                     /locus_tag="VFX94_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5766884.1"
                     /translation="MRTRRLFRHGLLPQLLVFEAVARLGSVTRAAEELHLAQPTVSLQ
                     LKKLAEALEVQLFEQRGRQLHLTGAGYALREACDELVHCLLRADARLEAWRRPKQHVL
                     RLAAEPESRAVAAQLLAEFCSRHPGVQGSLHVDERAELLARL"
BASE COUNT          453 a          961 c          977 g          414 t
ORIGIN      
        1 gccggcttcc atcgcttcga tgaggcgttc ggcgaacggc ttcttgcttg ccttgaaggc
       61 ccgggcggtt tgctccacga cctgggggcc ggccagcatg ttgccggcga cggaaaacgc
      121 tccgcccgcg accgcgccgc accagtcgac gcatttcgcg ccggtatgct gaccgattcg
      181 gccctcgtcg tcgacgacgt gcagttggcg gtggtcccgc ccttcgtcgg gcgaggtgag
      241 catcttcacc acgtcgggcg ccgagatgcc ggcgcgcagc aggtcgaggc cgtgccggcc
      301 gtagtaggga ttgacgagcg cctgggtgca gagggcgccc acgctgcttt cggcgtgcgg
      361 gcagagggcg cccaccgcga agaatcgcgt agcgaccgcc acgccgaacg cgccgctctc
      421 gtcgcgggcg atgatcgacc aggtcatggc cgcggatgat aacccgcgga ccgacccgtt
      481 attcaggcga agaggatgag ccccagcgtc gcgccaagcc cggccgcaat cccgtagcgc
      541 tcgagcgaac tcacttcctt tccggcgacg acgacgaccg gcgcgggcga agtctcgagg
      601 agcgagtgca ccactgcctg ctcgctcatc cgcgtggcgg agcgatagcg cgccgttccg
      661 agcacgatga gatccgggct ggtgtccaca gccgctgcca gaatcgcctt cgccttgtcc
      721 ccggcctgca gcttgacggt gtggcgcacg ccatgccact tgagcagctc ccgtgcggag
      781 gctacggact tctcggcgag ccccggcacg aaagcaagcg cgagccccga gcgcacgcaa
      841 agcagttgca cttcgggacg ctcgcggccc atgaactcgc gtacgacgta gcgaaccgca
      901 ggcagcgcgt tgaccgagtc caggaccgga acgaggatcc gcatggctat tctcctgcga
      961 gaagaagggc tgcaaggccc gccacgccgg cgggcagggc gtaacgctcg aacacgccgg
     1021 cccggccgcg cgcgagcacc gtgaccggga tgtcggtctg ctccatgacc gcctgcgcga
     1081 tcgagggatt tacccagcgc agccaggcgg gatggcgacc cacccccatc atcacctcgt
     1141 cgacgcgctc gcgttccgcg atcgcggcaa tgcgctcggc cggcttgccg atctcggcga
     1201 acgcgcggaa gggaatgttc ttcagcgcga gcgcctcgat ggcgcgcgcc atcgaggcgc
     1261 ggctgcgctc ggcgcgcagc gcgtcgcgcg cggcgcggct ggtgaactgc gccacgtggc
     1321 gatggaaacg cggctgcacg ttgacgagga ggacttccag cggcacgccg ctgcgctcga
     1381 gcgactccag gtaggaaagc ggtgcgagcg cggcttgcga agcgtctacg ggtaccagga
     1441 tcttcattgc atgacctcct tcaatcttgc tgccttgcga ttgcgcaggt agggaacggc
     1501 gagcacgccg ccgacgatca ccaggtagat cgccgagcgg atgcccggac gggcgtcgat
     1561 ccactcctgg tacagcggct cgtcggtgat cattttggcc gcggtccacg cgagcaccgc
     1621 gccgccgagg tacagcagag cggggaagcg ctcgatccag tgcaggatga gggtcgagcc
     1681 ccacaccacg atcgggatgc tgatgaggag ccccgccacc acgagcagca cgctgccgtg
     1741 ggcagcgccg gcgaccgcga gcacgttgtc gagacccatc acggcgtccg cgatcacgat
     1801 cgtgcgcatg gcggcccaga aactggtcgc gggtgcgacg tcgtggtcgc ggccgctttc
     1861 gtccccggcg agcaggcggt aggcaatcca ggcgaggagg atgcccccgg cgagcatcag
     1921 cccggggatt ccgagcagcc agagcaccac gacggtgagg ctcgcgcgca ccgcgaccgc
     1981 gccgacggtg ccccagatca ccgcgcggcg ctgcagcgtc ctcgggaggt tccgcgcggc
     2041 gagcgcgatc acgatcgcgt tgtcgccggc gaggacaagg tctatgacga tgatggccgc
     2101 gagcgctgac cagaactcgg gggcgaggaa gatctccatg ccccgagtgt gtgcggcgcc
     2161 gcagtagatg aaaattgacg ttcggcgatg gaagtcattg cgttccgcaa tggatccgcc
     2221 gcgaagttca cttacaatgc cggttccccg caggggagta gcttcccgcg ggatggccga
     2281 caattcgggg agaacatcct cccggtcatc ccggcagccc gatcccgcgc tgcaagccag
     2341 acctcggtta ccgcaaggag ccgagatgtc tcgcgtgcgc acgcgccgtt tgttccgaca
     2401 tgggctgctg ccccagctgc tggttttcga ggcggtggcc cgcctcggca gcgtcactcg
     2461 cgccgccgag gagctgcatc tcgcgcagcc tacggtgtcg ctgcagctca agaagctcgc
     2521 cgaggcgctc gaggtccagc tcttcgagca gcgcgggcgc cagcttcatc tcaccggcgc
     2581 gggctacgcg ctgcgcgagg cgtgcgacga gctcgttcat tgcctgctgc gcgcagacgc
     2641 gcgcctggaa gcctggcgcc ggccgaagca gcacgtgctg cggcttgcgg ccgagcccga
     2701 gtcgcgcgcc gtcgcggcgc agctcctcgc cgagttctgc tcccggcacc ccggggtgca
     2761 gggcagcctg cacgtcgacg agcgcgcgga gctcctcgcg cggct
//
LOCUS       DASUNT010000072         2620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_273016, whole genome shotgun sequence.
ACCESSION   DASUNT010000072 DASUNT010000000
VERSION     DASUNT010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273016"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1727
                     /locus_tag="VFX94_01515"
     CDS             <1..1727
                     /locus_tag="VFX94_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766885.1"
                     /translation="LVLLKFFGLHRAFSFAAATCAALFLTVGLGVELDVHKMTAGVFR
                     HGELDNSRDASILFAKDGKTATVHLVKYPDATSLRTNGKSDGSINMDPAGQRGSDEIT
                     MVLSAAMPLALRPETRTAAVIGIGTGLTTHTLLQSLDIERVETVEIEAAMAEASRGFM
                     PRNSGAFADPRGAILIDDAKSFFSTRNRRYDLIISEPSNPWVSGVSGLFTREFYRRIR
                     RHLNPDGMLVQWIQLYEIDASLVATVLGALGAEFPHYAVFGASDHDLLIMAAENPVPR
                     QAQARIFEHPGVAKELWTVHVLSAGDLDARYLGSRETLEPLFASYAMPANSDYAPVLD
                     LNAARHRFMEKSAADVVALLNAEVPLLELLEPQRSTRPVNPLFRGAYAFERVENTRVA
                     RYARDFLLRPRAPVAESLPRELQKDLELVKLRLIECRDPSELDVWTHSALRVARALNP
                     YLAPDELAPVWNRITTSACYGALNAFQQGWLALFQATGARDPARMAELAVRLMALDKP
                     LIADAREYLLLAAMAGYVASGDSASALELWTKYGEGARAARPAFRLLRCHAERSTCAN
                     AFSGYAER"
     gene            complement(1732..>2620)
                     /locus_tag="VFX94_01520"
     CDS             complement(1732..>2620)
                     /locus_tag="VFX94_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEX5766886.1"
                     /translation="AVEFTAKGAAVAARLEDGTEQRCKTRFVVDASGRDTFLASRMGI
                     KRRNKKHNSAAIYGHFTGANRLPGKAEGNISVFWFDHGWFWFIPLADGVTSVGAVCWP
                     YYLKTRETSTGQFLLDTIALCPALAERLREAKLVSPVTATGNYSYVADRTMGRNYLMV
                     GDAYTFIDPIFSTGVLFAMQGGFVAADTIQTCLHRPGQAAAALRRFDAAMRRGPAVFS
                     WLIYRMTTPAMRELFMDPTVKLRIQESVISVLAGDVFRRTPWRSRLLLFQAIYYFKSL
                     ALLRQSASAWLRRRRAVQG"
BASE COUNT          433 a          897 c          882 g          408 t
ORIGIN      
        1 ggttggtgtt actgaagttt ttcggccttc accgggcgtt ctcgttcgcg gccgcgactt
       61 gcgcagcgct cttcctcaca gtcgggctcg gcgtcgagct cgacgtgcac aagatgacgg
      121 cgggtgtttt ccgccacggc gagctcgaca actcgcgcga cgcctcgatc ctcttcgcga
      181 aggacggcaa gaccgcgacg gtgcatctcg tgaagtaccc ggacgcgacg agcctgcgca
      241 ccaacggcaa gtccgacggc tccatcaaca tggacccggc gggccagcgc ggcagcgacg
      301 agatcacgat ggtgctgagc gccgcgatgc cgcttgcgct caggcccgaa acgcgcaccg
      361 ccgcggtcat cggcatcggc accggcctca cgacccacac cctgctgcag agcctggaca
      421 tcgagcgcgt ggagacggtg gagatcgagg ccgcgatggc ggaggcatcg cgcggcttca
      481 tgccgcgcaa cagcggcgcg ttcgccgacc cgcgcggcgc gattctcatc gacgacgcga
      541 agagcttctt ctcgacccgc aaccgccgct acgacctcat catctccgag ccctccaacc
      601 cgtgggtgag cggggtgtcg ggccttttca cgcgcgagtt ctatcgccgc atccgccggc
      661 acctcaatcc ggacgggatg ctggtgcagt ggatccagct ctacgagatc gacgcctcgc
      721 tcgtcgccac ggtgctcggc gcgctcggcg ccgagttccc gcattacgcc gtattcgggg
      781 caagcgacca cgatctgctc atcatggcgg cggagaaccc cgtgccgcgg caggcgcagg
      841 cgcgcatctt cgagcatccg ggcgtcgcga aagagctgtg gacggtgcac gtcctgagcg
      901 caggagacct cgacgcgcgc tacctgggaa gccgcgagac gctcgagccg ctcttcgcga
      961 gctacgccat gccggccaac tccgattacg cgccggtgct cgacctgaac gccgcccgtc
     1021 accgcttcat ggagaagagc gccgccgatg tcgtggcgct cctgaacgcc gaggtcccgc
     1081 tcctcgagct gctcgagcca cagcgcagca cgcggccagt caacccgctc ttccgcggcg
     1141 cctacgcgtt cgagcgcgtg gagaacacgc gggtcgcgag gtacgcgcgc gacttcctgc
     1201 tgcgcccgcg cgcgcctgtc gccgagtcgc tgccgcgcga gctgcagaag gacctcgagc
     1261 tggtcaagct gcgcctcatc gagtgccgcg acccaagcga gctcgatgtg tggacgcaca
     1321 gcgcgctgcg cgtcgcgagg gcgctgaatc cgtacctcgc gcccgacgaa ctggccccgg
     1381 tatggaaccg catcacgacg agcgcctgct acggcgccct gaacgctttc cagcagggct
     1441 ggctggcgct ttttcaggcg accggtgctc gcgatcccgc acgcatggcg gagctcgcag
     1501 tccgccttat ggcgctcgac aagccactca tcgcggacgc gcgcgaatac ctgctgcttg
     1561 cggcgatggc ggggtacgtg gcgagcggcg acagcgcgag cgcgctcgag ctgtggacga
     1621 aatacggcga gggcgcgcgg gccgcgcggc cggcgttccg gctcctgcgc tgccacgccg
     1681 agcgctcgac ctgcgctaac gccttcagcg gctacgccga gcgctgagcg attacccctg
     1741 gacggcgcgc cggcgcctca gccaggcgga cgcgctctgg cgaagcaagg cgaggctctt
     1801 gaagtaatag atcgcctgaa acagcagcaa gcggctgcgc cacggcgtgc ggcggaaaac
     1861 gtctccggca aggaccgaaa tgaccgattc ctggatgcgc aatttcacgg tcggatccat
     1921 gaagagctcg cgcatggcgg gcgtggtcat ccggtagatg agccacgaaa aaactgccgg
     1981 cccccgccgc atggctgcgt cgaagcgcct cagcgcggct gcggcctggc ccggacggtg
     2041 gaggcaggtc tggatagtgt cggccgcgac gaagccgccc tgcatggcga acagaacgcc
     2101 ggtggagaat atcgggtcga tgaaggtgta cgcgtcgccg accatcaggt aattgcgtcc
     2161 catcgtgcgg tcggccacgt acgaatagtt gccggtggcg gtgacgggcg agacgagctt
     2221 tgcctcgcgc aggcgctccg cgagcgccgg gcacaacgcg atcgtgtcga gcaggaactg
     2281 cccggtgctc gtttcccggg tcttcaggta atagggccag cataccgcgc cgacgctcgt
     2341 gacgccgtcg gcaagcggga tgaaccagaa ccagccgtgg tcgaaccaga agacgctgat
     2401 gttgccctcg gccttgcccg gcaagcggtt cgcaccggtg aagtgcccgt agatcgcggc
     2461 gctgttgtgc ttcttgttgc gccgcttgat ccccatgcgg ctggcgagga aggtgtcgcg
     2521 accggatgca tccaccacga agcgcgtctt acagcgctgc tcggtcccgt cctcgagccg
     2581 cgcggccact gccgcgcctt tggcggtaaa ttcgaccgcg
//
LOCUS       DASUNT010000073         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_361142, whole genome shotgun sequence.
ACCESSION   DASUNT010000073 DASUNT010000000
VERSION     DASUNT010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361142"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VFX94_01525"
     CDS             <1..625
                     /locus_tag="VFX94_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX5766887.1"
                     /translation="SGEILAEEEFQAGAASPEAPVSGTEVALVANSSPSEGRATRARM
                     LFQILVASARESICIASPYFIPDRRLCEELVRAVRRGAAVTVLVPGRWNNHPLTRLAS
                     RRRYGRLLEGGVAIHEYQPAMMHAKILIVDHAWSVVGSTNFDNRSFGLNDEVNVAILQ
                     SELAAKLQADFQADLAASRAFTLEEWRGRPLAERAVAAFARLLERQA"
     gene            690..1508
                     /locus_tag="VFX94_01530"
     CDS             690..1508
                     /locus_tag="VFX94_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5766888.1"
                     /translation="MTYKTILLHVDNRPRRAERLALAFTLAERFQAHLVGLFALEAAR
                     IPSYALAETGQLVIEIERKRRVEDARAAEAQFRQAERRARTSAEWRLSTDDATAALRL
                     SARYADLVIAGQPEPDEPLGRAFAGELVLGAGRPVLFVPYAGRFADAGKRVMIAWNAS
                     REATRAVTDALPILARADSVEVVAFEPGGDHGEIAGADLAHFLARHGVKATAAHDVAP
                     GIEIGERILSRAADIDADLIVMGAYGRSRVRELVLGGATKGLLDAMTVPVLMAH"
     gene            1511..1690
                     /locus_tag="VFX94_01535"
     CDS             1511..1690
                     /locus_tag="VFX94_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766889.1"
                     /translation="MKKTKRKAKSPQRSAVPRPELKKVSRAQVGSDEEQRPYRPRPDD
                     ASIEDALEDWRDEEP"
     gene            complement(1831..>2015)
                     /locus_tag="VFX94_01540"
     CDS             complement(1831..>2015)
                     /locus_tag="VFX94_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766890.1"
                     /translation="DNHAMLHFVEKLGFEISPEPHERAIVRVVKNLGGEKRARPCAGE
                     APPGRARAPGAGIVPR"
BASE COUNT          327 a          702 c          682 g          304 t
ORIGIN      
        1 ctcgggcgag atcctggcgg aagaggagtt ccaggcgggc gcagcctcgc ctgaagctcc
       61 agtgtccggc acggaagtcg cgctcgtggc caacagctcg ccgtcggagg ggcgcgccac
      121 gcgcgcgcgc atgctcttcc agatcctggt tgcctcggcg cgcgagtcca tctgcatcgc
      181 ctcgccgtac ttcatcccgg accggagact atgcgaggag ctcgtgcgcg cggtgcggcg
      241 cggcgccgcc gtaacggtgc tcgtgccggg gcgctggaac aaccatcctc ttacgcgcct
      301 cgccagccgg cggcgctacg gccgcctgct cgagggcggc gttgcaatcc acgagtacca
      361 gccggcgatg atgcacgcca agatcctcat cgtcgatcac gcctggagcg tggtcggctc
      421 gaccaacttc gacaatcgct cgttcgggtt gaacgacgaa gtcaacgtag ccatcctgca
      481 aagcgagctc gcggcgaagc tgcaagccga cttccaggcg gacctcgccg cgagccgggc
      541 gttcacgctc gaagaatggc gcggtcgtcc gctcgctgag cgcgcggtcg cggcgtttgc
      601 ccgcctgctc gagcgccagg cctgaaacac cgcccggcgc ttgcgccaga tcaaatcgcg
      661 cgaagcccca cgtgacagac tgaaagtcca tgacctacaa gacgatcctc ctccatgtcg
      721 acaaccggcc gcgccgcgcc gagcggctcg cgctggcgtt cactctcgcg gagcgcttcc
      781 aggcgcacct agtggggctc ttcgccctcg aagcggcgcg cattccgtcc tacgcgctcg
      841 cggagaccgg gcagctagta atcgagatcg agaggaagcg ccgcgtcgaa gatgcacgcg
      901 ccgcggaagc gcaattccgc caagccgagc gccgcgcgcg cacgagcgcc gaatggcggc
      961 tctcgaccga cgacgcgact gccgcgctac gactgagcgc tcgctatgcg gacctagtga
     1021 tcgccggcca gcccgagcct gatgagccgc tcggccgggc gttcgcgggc gagctcgtgc
     1081 tcggcgcggg ccggccagtg ctgttcgtgc cctatgccgg tcgcttcgct gacgcgggca
     1141 agcgcgtaat gatcgcctgg aacgcgagcc gcgaggcgac ccgcgccgtg accgatgcgt
     1201 tgccgatcct ggcgcgcgcc gattccgtcg aggtggtcgc tttcgagcct ggcggcgacc
     1261 acggcgagat tgccggcgcc gacctcgcgc atttccttgc acgccacggc gtcaaggcaa
     1321 ccgcggcgca cgatgttgca cccggcatcg agattggcga gcgcatcctg tcgcgcgccg
     1381 ccgacattga cgccgacctg atcgtcatgg gcgcgtatgg tcgatcgcgc gtgcgcgagc
     1441 tcgtcctggg tggcgcgacg aagggcctcc tggacgcgat gaccgtgccg gtgctgatgg
     1501 cccattaaca gtgaagaaga cgaagcgcaa agccaaatcg ccacagcgca gcgccgtgcc
     1561 gcggccggag ctcaagaagg tgagccgtgc ccaggttggc tccgacgagg agcaacggcc
     1621 ctatcgccca cgcccggacg atgcatcgat cgaggacgcg ctcgaggact ggcgcgacga
     1681 ggagccctag cgctgcgtat gaacgcgtgg cgcaccttaa tggccgcacg tcccggccag
     1741 cgcttcacgc gctataacgc gcacgtgcag acgaggagca gcacgctgac tcccggacca
     1801 ggcttcctcg tgctctgatc ggcgcagcga tcatcgcggg acaatcccgg cgcctggcgc
     1861 gcgcgctcga ccgggcggag cttcgcctgc gcagggccgt gcgcgttttt cgccgccgag
     1921 gttcttgacg acgcgcacga tcgcgcgctc atgcggctcg ggcgaaattt cgaacccgag
     1981 cttctcgacg aagtggagca tcgcgtggtt gtcgc
//
LOCUS       DASUNT010000074         3559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_98034, whole genome shotgun sequence.
ACCESSION   DASUNT010000074 DASUNT010000000
VERSION     DASUNT010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98034"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VFX94_01545"
     CDS             <1..218
                     /locus_tag="VFX94_01545"
                     /EC_number="2.6.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922957.1"
                     /note="catalyzes the formation of pyruvate and
                     beta-alanine from L-alanine and 3-oxopropanoate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5766891.1"
                     /translation="RNCGLMGAIELEPRAGQPGKRAYEAFVKAFEAGVLLRVTGDIIA
                     LSPPLIVERAHIDRIVNCVGDMLRSTP"
     gene            581..874
                     /locus_tag="VFX94_01550"
     CDS             581..874
                     /locus_tag="VFX94_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX5766892.1"
                     /translation="MTPSLKYNDLMTAVIYGDPKTVEQLLALGKWPDKPDSRGSTALM
                     VATMLGDAASSEALLKAGANPNRPGPGGDTATSIARERRDGAVLKLLQRYGGK"
     gene            complement(923..2182)
                     /gene="speE"
                     /locus_tag="VFX94_01555"
     CDS             complement(923..2182)
                     /gene="speE"
                     /locus_tag="VFX94_01555"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEX5766893.1"
                     /translation="MNGLHLIGDLTGCRCDPQLLLDGAGFKARCLEMVAASGLTTMDA
                     TFHQFEGSGFTGTVVLAESHLAIHTWPERQGLTLDIYVCNYSADNSAKARKLFDAMVG
                     FFQPGEIARHEVERGAHLLMEPLNDSTGFYIKANAQLGEWQTRFQKLAIYDTPHYGKI
                     FRLDGFNMTSEKEEFVYHENLVHPALTAHACPRKVLIIGGGDGGSSEEALKHPSIDSV
                     TMVEIDADVIEVAKKHFHAVHQGVFDNPKLRVLVEDGMRFVRETQERFDLIALDLNDP
                     MGPAEALYSTEFFQQLRQALAPGGALTLHIGSPVARPERVAQLAQRLNGVFRIVRPYT
                     MYIPLYGSQWAMAVCSDKLDPKAFTADEIDRRIEARKLGDLKYYNGETHEGVFALPNF
                     VRDLVVPARLKQPARGRRLGVVRAAAK"
     gene            complement(2313..3212)
                     /gene="nudC"
                     /locus_tag="VFX94_01560"
     CDS             complement(2313..3212)
                     /gene="nudC"
                     /locus_tag="VFX94_01560"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEX5766894.1"
                     /translation="MSDLPFRAPLEFEPLVCAEPSGEALWFVFRGDKLLVELGPPERP
                     SDDPRFRLRPAWARLPWLKDHEALHEQASRRLYLGRLNRVDCFAAELAPQAEVPAGLA
                     LEGLRPLFSVLDDDHFALAGRALQLLEWDRNHQFCGRCGAPTEPKREERVRLCPACKL
                     AAYPRVAPAVMALVRRHDEILLARSPHFPPGMYSALAGFVEPGESLEQCLAREVAEEV
                     GVRVAAPRYIASQSWPFPHSLMIAFVCEWQSGEIRPQAGEIEDAKWFKVLQLPKLPSK
                     ISIARRLIDGVCQDIVRDIEGQT"
     gene            3250..>3559
                     /locus_tag="VFX94_01565"
     CDS             3250..>3559
                     /locus_tag="VFX94_01565"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192262.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX5766895.1"
                     /translation="MLSPAVAPTAPSANSSATDSELANAIRALAMDAVEAAKSGHPGM
                     PMGMAEIAVALWKRHLRHNPANPAWVNRDRFVLSNGHGSMLLYSLLHLTGYELPMEEL
                     K"
BASE COUNT          599 a         1190 c         1184 g          586 t
ORIGIN      
        1 tccgaaattg cggcttgatg ggggccatcg agctcgagcc ccgggccggg cagcccggca
       61 agcgagccta tgaggcattc gtcaaggctt tcgaggccgg cgtactgctc cgggtcacgg
      121 gcgacatcat cgccctttcg ccgccgctca tcgtcgagcg cgcccatatc gaccgcatcg
      181 tgaactgcgt cggcgacatg ctgcgctcga ccccttaggg ttatcagcgg gccctggcag
      241 aggggaggag ggtacacaag cagcctgaga aggcgggaga ggcaaaatga ccgcaggaaa
      301 accgaattac cgcggtttcc atacgacgtt ccttgcgtgc ttcgttgccg cgctttccgc
      361 ggccacccct gtgctggcga acgaaccagc caccgaagaa ggggcgtctc tgccggccga
      421 gcttgcccaa gccccggcca tcgaagctac cgcgctgccc gcggagcctc cagaggagga
      481 cacgccgaag gctgcggccg acaccccggc tgtaccgccg actcccccag gcgcggaagg
      541 acttccgccg cccgcccggc cgcggatgat ttccgggccg gtgactccgt cgttgaagta
      601 caacgacctc atgacggcgg tgatctatgg cgatccgaaa accgtcgagc agcttctcgc
      661 gctcggcaag tggcccgaca agcccgacag ccgtggcagc acggccctca tggtggccac
      721 catgctcggc gacgcggcca gcagcgaggc gctgctcaag gcgggagcca atccgaaccg
      781 gccaggaccg ggaggcgaca ccgcgacttc catcgcgcgc gagcgccggg acggcgccgt
      841 gctgaagctt ctccagcgct acgggggcaa gtaagcaaaa gggggcgacc ggcccccctt
      901 ttccttccct cgctttttct tcttactttg ccgcggccct gaccacgccc agccggcggc
      961 cgcgggccgg ctgcttcaag cgcgccggaa cgacgaggtc gcgaacgaag ttgggcagcg
     1021 cgaacacgcc ctcgtgggtc tcgccgttgt agtacttgag gtccccgagc ttgcgcgcct
     1081 cgatgcgccg atcgatctcg tcggcggtga aagccttcgg atcgagcttg tcggagcaca
     1141 ccgccatggc ccactgcgag ccgtaaagcg ggatgtacat ggtgtacggc cgcacgatgc
     1201 ggaacacgcc gttcagccgc tgggctagct gggcgacacg ctccgggcgt gctaccggcg
     1261 agccgatgtg cagcgtgagc gctccgccgg gcgcgagcgc ctggcgcagc tgctgaaaga
     1321 actcggtgga gtagagcgcc tccgccggcc ccatcgggtc gttcaggtcg agcgcgatca
     1381 ggtcgaagcg ctcctgcgtt tcacgcacga acctcatccc gtcctcgacc agcacccgca
     1441 gcttcgggtt gtcgaacacg ccctggtgga cggcatggaa gtgcttcttg gcgacctcga
     1501 tcacgtcggc gtcgatctcc accatcgtga cgctgtcgat ggacgggtgc ttgagcgcct
     1561 cctcggagga accgccgtcg ccgccgccga tgatcagcac cttgcgcgga caggcgtgcg
     1621 ccgtgagcgc ggggtggacg aggttctcgt ggtagacgaa ctcctccttc tccgaggtca
     1681 tgttgaaccc gtcgaggcgg aagatcttgc cgtagtgcgg cgtgtcgtag atcgccagct
     1741 tctggaagcg cgtctgccac tcgccgagct gggcattcgc cttgatgtag aagccggtcg
     1801 agtcgttcag cggctccatc agcaggtgtg cgccgcgctc cacctcgtgg cgggcgatct
     1861 cgccgggctg gaagaagccg accatcgcgt cgaagagctt gcgcgccttg gcggaattgt
     1921 ctgcgctgta gttgcagacg tagatgtcga gcgtcagccc ctggcgctcg ggccaggtat
     1981 ggatcgcgag gtgtgactcg gcgagcacca cggtgccggt gaagccggag ccctcgaact
     2041 ggtggaaggt ggcgtccatc gtggtgaggc cggacgcggc caccatttca aggcaccgcg
     2101 ccttgaagcc cgcgccgtca agaaggagct gcggatcgca gcggcagccg gtgaggtcac
     2161 cgatgagatg cagaccgttc atttaacgta ccctccaaaa tgcgaaggcg cagcctgccg
     2221 cgtatcggga gtccaggcca cgccttccat tcgcgccttt cggcgcacag tgctcgtcga
     2281 tcgagctcaa ctcgtgcaaa acctcctagg tctcaagttt gcccttcgat gtctcgaacg
     2341 atgtcttggc agacgccgtc gatcaatctt cgagcgatcg agattttgct tggaagtttc
     2401 ggcaattgca agactttgaa ccactttgcg tcctcgatct ctcccgcctg cggccggatc
     2461 tcgccgcttt gccactcgca gacgaacgcg atcatgagcg agtgcgggaa gggccatgac
     2521 tggctcgcga tgtagcgcgg ggccgcgact cgcacgccga cctcctcggc cacctcgcgc
     2581 gcgaggcact gctcgagcga ctcgcccggc tcgacgaagc ccgcgagcgc gctgtacatg
     2641 ccgggcggaa agtgggggct gcgtgcgagc aggatctcgt catgcctgcg caccagcgcc
     2701 atgacggccg gcgcgacgcg cgggtaggcg gcaagcttgc aggcgggaca taggcgcacg
     2761 cgctcttcgc gcttgggctc ggtgggcgcg ccgcagcggc cgcagaactg atggttgcgg
     2821 tcccactcga ggagctggag cgcccggccc gccagggcga agtgatcgtc atccagcacc
     2881 gagaagagcg gccgcagccc ctcgagcgcc aggccggcgg gtacttcggc ctgcggcgcg
     2941 agctcggcgg cgaagcagtc cacgcggttg agtcgcccga gatagagccg cctcgaggcc
     3001 tgctcgtgca gcgcttcgtg gtctttcagc cacggcagcc gcgcccaggc tggccgcagg
     3061 cgaaagcgcg gatcgtcgct cgggcgctcg ggcgggccca gctcgacaag cagcttgtcg
     3121 ccgcggaaca cgaaccaaag cgcctcgccg ctcggctcgg cgcagacgag tggctcgaat
     3181 tcgagcggcg cgcggaaggg gagatcgctc atttgaccct ctcgcggtga gtataatttg
     3241 ccgcttccga tgctttctcc cgcagtcgca ccgacggctc cgtcggcgaa ctcgagcgct
     3301 accgattctg aattagccaa cgccatccgc gcgctcgcca tggatgcggt ggaggcggcg
     3361 aaatccggcc acccgggcat gccgatgggc atggccgaga tcgcggtcgc gctctggaag
     3421 cggcacctgc gccacaaccc ggccaatcct gcctgggtga accgcgaccg cttcgtgctc
     3481 tcgaacgggc acggctcgat gctcctctac agcctgctcc acctcaccgg ctacgagctg
     3541 ccgatggagg agctcaaaa
//
LOCUS       DASUNT010000075         2794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_624062, whole genome shotgun sequence.
ACCESSION   DASUNT010000075 DASUNT010000000
VERSION     DASUNT010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624062"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFX94_01570"
     CDS             complement(<1..452)
                     /locus_tag="VFX94_01570"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5766896.1"
                     /translation="MAARSLRAHLLRLLLPPIAALLAVGAVVAYYPSLEPATQAYDQA
                     LVDIGLAIGSQVRVTETQYRFELPQAVDQVLRTDRFDRIFYRITSPSGIEIAGDPELP
                     PPPSDQIAHNTRYDGRQVRVVTVQSPCGSSICTVLVGETTLKRQRLAR"
     gene            complement(462..1142)
                     /locus_tag="VFX94_01575"
     CDS             complement(462..1142)
                     /locus_tag="VFX94_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5766897.1"
                     /translation="MRLLIVEDDAPLASGLQRILEAEGHAVDVTARGEEAVAAASNEK
                     FDLVILDIGLPHMDGFEVLRRLRSRVSDKSGPTPVLVLTARDAVEDRVRGLDLGADDY
                     MVKPFAMPELTARVRALLRRSQAHGGPRIAHGPLTLDTVARRAFLRNEPLELAAREWA
                     VLEVLLAKVEKIVSKEAIIQAVAGWGDDLSPNAIEVYVSRLRSKLEPAGIKIRTVRGF
                     GYMLEEFK"
     gene            1219..1698
                     /locus_tag="VFX94_01580"
     CDS             1219..1698
                     /locus_tag="VFX94_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5766898.1"
                     /translation="MTIKVSMKVNGKPVSAEVEARTLLVSYIRENLRLTGTHVGCDTA
                     QCGACTVLMNGRAIKSCSIVAMQAEGADIMTIEGVAKPDGTLHPMQAAFKENHGLQCG
                     FCTPGMVMNALDFAQSHPNPTEQEVREALEGNLCRCTGYHNIVKSIVAGAKAMGGKL"
     gene            1695..>2794
                     /locus_tag="VFX94_01585"
     CDS             1695..>2794
                     /locus_tag="VFX94_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5766899.1"
                     /translation="MNSSGNAPLIGARVTRKEDYRFLTGTGQYTDDVALPNQAYAAFV
                     RSPHAHAVLKKIDFEKAKKAPGVIGVFTGADLAAAKVGGLPCGWLITDVKGQPMKEPP
                     HPCLAQDKVRYVGDHVAVVIAETLDQARDAAELVKVDYEELAAVTDAAKARAAKPIHD
                     IAPDNTCYVWSIGDKAKFDEAFKSAAHVTRLEFVNNRLIPNAIEPRAAIGSFSRADDA
                     YTLYVSSQNPHVERLLMTAFVLGLPEHKVRVVAPDVGGGFGSKIYLYAEDVVVTWASK
                     QLNRPVKSTSDRSEAFVTDAHGRDHVTVAELALDKDGKFLAMRVKTTANLGAYLSTFA
                     SCIPTILYATLLAGQYKTPQIYCEVTAVFTNTT"
BASE COUNT          503 a          964 c          890 g          437 t
ORIGIN      
        1 tcgcgcgcga ggcgctggcg cttgagcgtg gtctcgccga cgagcacggt gcagatcgag
       61 ctcccgcacg gcgactgcac cgtcaccacg cgcacttgcc tgccgtcgta ccgggtgttg
      121 tgcgcgatct gatcgctcgg cggcggcggc agctcagggt cgccggcgat ctcgattccc
      181 gacgggctcg tgatgcggta gaagatgcga tcgaagcggt cggtgcgcag gacctggtcc
      241 accgcctgcg gcagctcgaa gcggtactgg gtctccgtga ctcgcacctg cgaaccgatg
      301 gcaaggccga tatcgacgag cgcctggtcg tatgcttgcg tggcgggctc gagcgacggg
      361 tagtacgcca ccaccgcacc gaccgcaagc agcgccgcga tcggcggcag cagcaggcgc
      421 agcaggtgcg cgcgcaggct tcgcgcggcc atcgcgtccc cttacttgaa ctcttccagc
      481 atatagccga agccgcgcac ggtgcggatc ttgattccgg ccggctcgag cttggagcgc
      541 aggcgcgaga catagacctc gatcgcattt ggcgaaaggt cgtctcccca gccggcgacc
      601 gcctggatga tcgcctcctt cgagacgatc ttctccacct tggcgagtag cacttcgagc
      661 accgcccact cgcgcgccgc gagctccagg ggctcgttgc gcaaaaaagc ccgtcttgcg
      721 accgtgtcga gcgtcaatgg cccatgagcg atgcgcgggc cgccgtgcgc ctgcgagcgg
      781 cgcaagagcg cgcgcaccct agcggtgagc tccggcatcg cgaagggctt caccatgtag
      841 tcgtcggccc cgaggtcgag gccgcgcacg cggtcttcca ccgcgtcgcg cgccgtaagc
      901 acgagcactg gcgtcggccc gctcttgtcc gagaccctgg agcgcagccg gcgcagcacc
      961 tcgaatccgt ccatgtgcgg caggccaatg tccaggataa cgaggtcgaa cttttcgttc
     1021 gacgcggcgg ccaccgcctc ctcgccgcgc gcggtgacgt cgaccgcgtg cccctcggcc
     1081 tcgagaatgc gctgcagccc cgatgcaagc ggggcgtcgt cctccacgat cagcaatcgc
     1141 atggccggcg gcgagaaatg gcagtggcat aaatcatacc gccgcgctac gatatttttc
     1201 tccccggagg agccccgcat gacaatcaag gtatccatga aggtcaatgg caagcccgtt
     1261 tctgccgagg tcgaggcgcg cacgctgctt gtcagctata ttcgcgagaa cctgcggctg
     1321 accggcaccc acgtcggctg cgacacggcg cagtgtggcg cctgcaccgt gctcatgaac
     1381 ggccgcgcga tcaagagctg ctcgatcgtg gcgatgcagg ccgagggtgc cgacatcatg
     1441 acgatcgagg gtgtggcaaa gcccgatggc accctgcacc ccatgcaggc cgcgttcaag
     1501 gagaaccacg gtctgcaatg cggtttctgc acccccggga tggtgatgaa cgcgctcgac
     1561 ttcgcccaaa gccatcccaa ccccaccgag caggaagtgc gcgaagcgct cgagggcaac
     1621 ctttgccgct gcaccggcta ccacaacatc gtgaagagca tcgtcgccgg cgccaaagcc
     1681 atgggaggaa aattgtgaac tcctccggga acgccccgct gattggcgcg cgcgttacgc
     1741 gcaaagagga ttaccgcttc ctcaccggca ccggccagta caccgacgat gtcgccctgc
     1801 cgaatcaggc ttacgccgcc ttcgtgcgct cgccgcatgc ccatgcagtc ctgaaaaaaa
     1861 tagattttga aaaagccaag aaggcccctg gggtgatcgg cgtcttcacc ggcgccgacc
     1921 tggccgccgc caaggtcggc ggcctgccgt gcggctggct gattaccgac gtcaagggcc
     1981 agccgatgaa ggagccgccg cacccctgcc tcgcgcagga caaggtgcgc tacgtcggcg
     2041 accatgtggc ggtggtgatc gccgagacgc tcgaccaggc gcgcgacgcg gccgagctgg
     2101 tgaaggtgga ttacgaggag cttgccgcgg tgaccgatgc cgccaaggcg cgcgccgcga
     2161 agcccatcca cgatatcgcg ccggacaaca cctgctacgt ctggtccatc ggcgacaagg
     2221 cgaaattcga cgaggcgttc aaaagcgcgg cgcacgtcac ccggctcgaa ttcgtcaaca
     2281 accgcctgat cccgaacgcc atcgagccgc gcgcggcgat cggctcgttc agccgcgccg
     2341 acgacgccta cacgctgtac gtttccagcc agaacccgca cgtcgagcgg ctgctgatga
     2401 ccgcgttcgt gctcggcctg cccgagcaca aggtgcgcgt ggtggcgccc gacgtcggcg
     2461 gcggcttcgg ctcgaagatc tatctctacg ccgaggacgt ggtggtgacc tgggcgtcga
     2521 agcagctcaa ccggccggtg aagtcgactt ccgaccgctc cgaggccttc gtcaccgacg
     2581 cgcacggccg cgaccacgtc acggtggccg agctcgcgct cgacaaggac ggcaagttcc
     2641 tcgccatgcg ggtcaagacc accgcgaacc tcggcgctta cctctccacc ttcgcttcct
     2701 gcatcccgac catcctgtac gcgacgctgc tcgcagggca atacaagacg ccgcagatct
     2761 actgcgaggt gaccgcggtg ttcaccaaca ccac
//
LOCUS       DASUNT010000076         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1150573, whole genome shotgun sequence.
ACCESSION   DASUNT010000076 DASUNT010000000
VERSION     DASUNT010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150573"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..1080
                     /locus_tag="VFX94_01590"
     CDS             7..1080
                     /locus_tag="VFX94_01590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5766900.1"
                     /translation="MRALSKHYTLTLVVNTGDDALLSRHGIPAELVRLGIERAIAPLA
                     DAAALARLGRLFVRQRFDGVVSLAPKAGLLAMAAARFARIPFRCHVFQGEVWASARGV
                     RRALLKSLDRFVAALATHLLVISASERSVLEREGIVQPGQAELIANGSLCGVDLDRFR
                     ADPQWRGDARSELGLPRGALVVLFLGRITRDKGVLDLAHAFRAVAGALPQAHLVYVGP
                     DEQGLEPALRAAVGEHSARLRIRGLTDAPERYIAAADVLALPSYREGFGNVLIEAAAA
                     GVPGLASRIYGIEDALRDGETGLLHPPGDVGAIAAGLRRLFEDAPLREKLGRQARERA
                     AREFGRENTIAFWIGFLQANTVR"
     gene            1077..>2132
                     /locus_tag="VFX94_01595"
     CDS             1077..>2132
                     /locus_tag="VFX94_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766901.1"
                     /translation="MSTSQTPSAPAEVGMRSRSVKAIGITGLMLAVQMVTYLFAVRHI
                     LASLGTESFGTFQLMRQVTTFAMPVLLLGLNVALVQRLGTARGNPAGERQAMLAAALV
                     AGVAWCLVAILRASDAGWLSALLLSRPEAEAAQSLLGLLGAVLAYNYVYALYRGRQKF
                     LVASALQAACFGAVPLLFAFHYRADGVYAITNGIAVAIFLISMAGVVPQLRAFGRAAL
                     PETGVLKQELAIQVHYGARRMFIPLLLGAMMALGPIALTHADLGGEAGVYLVALVVFR
                     AIDPALTIVAVVMLPMLSQASAYRQARAADYARLALHVTLGVGGFLSVVGALLAGWLA
                     AAFFGPEEIAVPLRVLVF"
BASE COUNT          275 a          717 c          738 g          402 t
ORIGIN      
        1 gatctcctgc gcgcgctctc gaagcactac acgcttactt tggtcgtcaa tacaggcgac
       61 gacgccttgt tgtcgcgcca cggcattccg gccgagctcg tgcggctcgg catcgagcgt
      121 gccatcgccc cgctggccga cgctgccgcg cttgcgcgcc tggggcggct gtttgtgcgc
      181 cagcgcttcg atggtgtggt gtccctggcc cccaaagccg gactgctcgc catggcggcg
      241 gcgcgattcg cccggatccc ttttcgctgt cacgtgtttc aaggcgaagt ctgggcaagc
      301 gcgcgcggcg tgcgccgtgc gctgctgaag tcgctcgatc ggttcgtggc tgcgctcgcc
      361 acccacctgc tggtcatcag cgcctcggag cgctcggtgc tcgagcgcga aggcatcgtt
      421 cagcccgggc aggccgagct gatcgcgaac ggctcgcttt gcggggtgga tctggacaga
      481 tttcgcgccg acccgcagtg gcggggcgac gctcgcagcg agcttggtct tccgcggggc
      541 gcgctggtgg tattgttcct cggccgcatt acgcgcgaca aaggcgtgct cgatctcgcg
      601 catgccttcc gcgccgtggc gggagcgctg ccccaggcgc atctggtcta cgtcggcccc
      661 gacgagcagg gtctggagcc ggcgctgcgc gcggccgtgg gagagcacag cgcgcggctg
      721 cgcatccgtg gcctgaccga cgccccggag cgctatatcg cggcggcgga cgtgctcgcc
      781 ctgccgagct accgcgaggg ctttggtaat gtgctcatcg aagcggcggc ggcgggcgtg
      841 ccaggcctcg cctcgcggat ctacggcatc gaggacgcac tgcgcgatgg cgagacgggc
      901 ctgctgcacc cgcctggcga cgtcggcgct atcgcggcgg gcctgcgccg cctgttcgag
      961 gacgcgcctt tgcgcgagaa gctgggacgc caggcgcgcg agcgagcagc ccgcgagttc
     1021 ggtcgcgaga acacgatcgc gttctggatc ggttttcttc aggcgaacac ggtgcgatga
     1081 gcacctcgca aaccccgtcg gcccccgccg aagtcggcat gcggtcgcgc tcggtaaagg
     1141 cgatcgggat caccggccta atgctagccg tccagatggt cacctacctg ttcgcggtcc
     1201 gtcacatcct cgccagcctg ggcacggaat cgttcggcac gtttcagctc atgcggcagg
     1261 taacgacgtt cgcgatgcca gtactgctgc tcgggctaaa cgttgcgctg gtgcagcgcc
     1321 ttggaaccgc gcgcggcaac cctgccggcg agcggcaggc gatgctggcg gcagcactcg
     1381 tcgccggcgt cgcctggtgc ctggttgcaa tactgcgtgc gagcgacgcc ggatggttaa
     1441 gcgcgctgct gctgtcgcgg cccgaggcgg aagcggcgca gtcgctcctc ggactactgg
     1501 gtgccgtgct tgcctacaac tacgtctacg cgctctaccg tgggcgccag aagtttctcg
     1561 tcgcgagcgc gctgcaagca gcctgcttcg gcgcggttcc gctgctgttc gcgttccatt
     1621 accgggcgga cggggtttac gccattacga acggcattgc agtcgccatc tttctcatat
     1681 ccatggcggg agtagttccc cagctgcgcg ccttcggccg agcagcgctg ccggagacgg
     1741 gcgttctcaa gcaggagctc gccatccaag tgcactacgg cgcgcgccgc atgttcatac
     1801 cgctgctgct cggcgccatg atggccctcg ggccgatcgc gcttacgcac gcggacctag
     1861 gcggcgaagc gggcgtctac cttgtcgccc tggtggtgtt tcgggccatc gatccggccc
     1921 tgacaatcgt agctgtggtc atgctgccga tgctctccca ggcgagcgcc taccgccagg
     1981 cgcgagcggc cgattatgcg cggctcgcgc tgcacgttac tttgggcgtg ggcggtttct
     2041 tgtccgtcgt cggcgcgctg ctcgccggct ggctggccgc cgcgttcttc ggtcctgaag
     2101 agatcgctgt tccgctgcgg gtcctggttt tc
//
LOCUS       DASUNT010000077         4059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_624173, whole genome shotgun sequence.
ACCESSION   DASUNT010000077 DASUNT010000000
VERSION     DASUNT010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624173"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VFX94_01600"
     CDS             complement(<1..1163)
                     /locus_tag="VFX94_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5766902.1"
                     /translation="MSRRWLPYLVAVLAVAAALALRAALDPWLGARTPYVTLFGAVIV
                     AAWYGGFAPALVAAVLGWLGSDVLFVEPRGRISFRGTAQLIELAAYACSTLLIAALGG
                     AMRRARQRSEESEQRFRDFMQNSPNGVFLKDEEGRYLFINRTGERLAGGTDWLGKTDA
                     ELLDGRVAADIRDHDRAVLESNAPQRYDLEFPAAEGARTLRSVKFPLRDAGGRRYVGS
                     ITADVTEQVRSEAALREAQRKLQTVADALPAAVTLCSRDLRYLWINQYGAQWLRRTPR
                     EVIGKRMDEVIGADQLAQIRPFIERVLAGEHVSYEREVDYPGIGRRWASVRFAPASEE
                     SWVAVITDIHERKRMEQALREADRRKDQFIATLAHELRNPLAPIRSAVEILARA"
     gene            1217..2542
                     /locus_tag="VFX94_01605"
     CDS             1217..2542
                     /locus_tag="VFX94_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5766903.1"
                     /translation="MLQERIPDLSKCVEPDRVRREAYTDPQIFELEMQRIHERVWIYC
                     GHESQIPKAGDYYTVQIGRQPIIMVRGSDGKISVLYNRCPHRGNMMCGDRHGNTGEFF
                     RCSYHAWTFHHDGRLKNIPMMESGYSGTRYGRDNPDCSMKPAARVDTYRGFVFASLAK
                     DGPSLKEFLGKAIVAFDDMCDRAPEGEVEVVPNCFRVIQHSNWKIFLENQLDALHPSV
                     THQSTGRAAHEVEKALAKKARKGEVPLSYHMLSAFATVTIDKWDAFQTLNYPYGHCIL
                     TGYMGLRPKDPDTLAYDKIMIKAYGQKRFEEIVGVNIHHVLIYPGLSVQSPLQQLRAV
                     RPLGVDSTLTEIWHFRLKGAPEAIYRRSLAYYNLVNSPSTLVNADDLENFWKCHQGLS
                     SDGGDWVSFGRHLGRDIAKDGHVETAPNCGTSEAPMRNQMKAWAEYMGG"
     gene            2535..3017
                     /locus_tag="VFX94_01610"
     CDS             2535..3017
                     /locus_tag="VFX94_01610"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEX5766904.1"
                     /translation="MARDVQHQVEQFLYHQADLLDRKQWEKWIGLFTEEGVYWMPPDP
                     SYTTWDGTPAIFAEDKNLMRVRMGRVLHPDAWSQRPLWGTNHVVSNVIIEKQSPNGDV
                     IARSRFHMMELRRDDVRHFAGSYFHHLVRTKTGYRIKLQRVDMTNAQAAYDYVLQVWV
                     "
     gene            complement(3196..3741)
                     /locus_tag="VFX94_01615"
     CDS             complement(3196..3741)
                     /locus_tag="VFX94_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766905.1"
                     /translation="MAFEHALSGHAMCERAQTPVTAPGQTAKMSDAWLRRLVMLATVL
                     LVLHAVGSWAYAALGVPAAIVSAGLVGAVSIFSARMAGLGEGNSAWFVVPTMVFTVLP
                     LGARLWTLVTTEQGWWARSIEFAPFLIGFAAPVLLLLTAYLELGRRERGGEPSAIANT
                     AADATRVSPPEAARIPFRQSL"
BASE COUNT          735 a         1376 c         1305 g          643 t
ORIGIN      
        1 gcgcgcgcga ggatctcgac cgcgctgcgg atcggcgcga gcgggttgcg cagctcgtgc
       61 gcgagcgtgg cgatgaactg gtccttgcgc cggtcggcct cgcgcagcgc ctgctccatg
      121 cgcttgcgct cgtggatgtc ggtgatcacc gccacccagc tttcttcact ggcaggcgcg
      181 aagcgcacgc tcgcccagcg ccggccgatg ccgggatagt cgacctcgcg ctcgtagctc
      241 acgtgctcgc cggcgagcac gcgctcgatg aagggcctga tctgcgcgag ctggtccgcg
      301 ccgatcactt cgtccatccg cttgccgatg acctcccgcg gcgtgcgccg cagccactgc
      361 gcgccgtact ggttgatcca gagatagcgc aggtcgcggc tgcagagcgt cactgccgcc
      421 ggcagcgcgt cggcaaccgt ctgcagcttg cgctgcgcct cgcgcaacgc ggcttcgctg
      481 cgcacctgct cggtgacgtc ggcggtgatc gagccgacgt agcgccggcc cccggcgtcg
      541 cgcagcggga atttcacgct gcgcagcgtg cgcgcgccct cggcggccgg aaactcgagg
      601 tcgtagcgct gcggcgcgtt gctctcgagc accgcgcggt cgtggtcgcg gatgtctgcg
      661 gcgacgcgcc cgtcgagcag ttcggcatcg gtcttgccga gccagtcggt gcctccggcc
      721 aggcgctcgc cggtgcggtt gatgaacaga tagcgcccct cctcgtcctt gaggaaaacg
      781 ccgttcggcg agttctgcat gaagtcgcgg aagcgctgct cgctctcttc gctgcgctgg
      841 cgcgcgcggc gcatcgcgcc gccgagggcg gcgatgagaa gcgtggaaca cgcgtaggcc
      901 gcgagctcga tcagctgcgc cgtgcctcgg aagctgatgc gcccgcgcgg ctccacgaac
      961 agcacatcgc tccccagcca tccgagcacc gctgcgacga gcgcgggggc gaaaccgccg
     1021 taccaggcgg ccacgatcac cgcgccgaac aacgtgacgt acggcgttcg ggcaccgagc
     1081 cacgggtcga gcgccgcgcg cagcgcgagc gcggcggcta cggcgagcac ggcgacgaga
     1141 tagggcagcc agcgtcgaga caagccactc ctcctgcgta gggcctggat tattattgcc
     1201 gccgaggaga tttgtcatgc ttcaagagcg catacccgac ttgtccaagt gcgtcgagcc
     1261 cgaccgcgtg cggcgcgagg cgtacaccga cccgcagatc ttcgagctcg agatgcagcg
     1321 catccacgag cgcgtgtgga tctactgcgg ccacgagagc cagatcccga aggcgggcga
     1381 ctactacacc gtgcagatcg gccgccagcc gatcatcatg gtgcgcggct cggacgggaa
     1441 aatcagcgtg ctctacaacc gctgcccgca ccgcggcaac atgatgtgcg gcgaccgtca
     1501 cggcaatacg ggagagttct tccgctgctc gtatcacgcc tggaccttcc atcacgacgg
     1561 gcgcctgaag aacattccga tgatggaatc gggctacagc ggcacgcgct atggccgcga
     1621 caaccccgac tgcagcatga agcccgccgc gcgggtcgac acttatcggg ggttcgtctt
     1681 tgcgagcctc gcgaaagacg gtccttcact aaaggaattt ctcgggaagg ccatcgtcgc
     1741 cttcgacgac atgtgcgatc gcgcacccga gggcgaggtc gaggtggtgc cgaactgctt
     1801 tcgcgtcatc cagcactcga actggaagat cttcctcgag aaccagctcg acgcgctgca
     1861 tccctcggtg acgcaccagt ccacgggccg cgccgcgcac gaagtggaga aggcgctcgc
     1921 gaagaaggcg aggaagggcg aggtgccgct ctcctaccac atgctctccg cgttcgcgac
     1981 ggtgacgatc gacaagtggg acgcgttcca gaccctcaat tacccgtacg gtcactgcat
     2041 cctgaccggc tacatgggcc tgcggccgaa ggaccccgac accctcgcct acgacaagat
     2101 catgatcaag gcgtacgggc agaagcgctt cgaggagatc gtgggcgtaa acatccacca
     2161 tgtcctgatc taccccgggc tgtcggtgca atcgccgctg cagcagctgc gcgcggtgcg
     2221 cccgctcggc gtcgatagca cgctcaccga aatctggcac ttccggctga agggagcacc
     2281 cgaggcgatc taccggcgct cgctcgccta ctacaacctg gtgaactcgc cctcgacgct
     2341 ggtcaacgcg gacgacctcg agaacttctg gaagtgccac caggggctgt cctccgacgg
     2401 cggcgactgg gtcagcttcg gccgccacct cgggcgcgac atcgccaagg atggccacgt
     2461 cgagaccgcg ccgaattgcg gaacctccga agcgccgatg cgcaaccaga tgaaggcgtg
     2521 ggcggagtac atgggtggct agggacgtac aacaccaggt cgaacaattc ctctaccacc
     2581 aggccgacct gctcgaccgc aagcagtggg agaagtggat cgggcttttc accgaagagg
     2641 gggtctactg gatgccgccc gatccctcct acaccacctg ggacggcacg cccgcgatct
     2701 tcgccgagga caagaacctc atgcgcgtgc gcatgggccg cgtgctccat cccgacgcct
     2761 ggtcgcagcg cccgctctgg ggcacgaacc atgtggtttc caacgtcatc atcgaaaaac
     2821 aatcccccaa cggcgacgtg atcgcccgtt cccgcttcca catgatggag ctgcgccgcg
     2881 acgacgtgcg ccatttcgcg ggttcttatt ttcatcattt ggtaagaaca aagaccggct
     2941 atcgcataaa gctgcagcgc gtggacatga ccaacgcgca ggcggcttac gactacgtcc
     3001 ttcaggtctg ggtgtgaagc cattgcctat gcaggcgcgg tcgggcgtca ggcggcgaac
     3061 cgaagagcgc cgcaccgcgg tgcgtcaagc ctagccacgg gtggcctcat agctgcggct
     3121 cgatcgagcg cgggtgaacc gcgtcagcgt tcgaaggtag atggcaagct ggccggcgcc
     3181 cgacggcgca tcaactcaga gcgattgccg gaaaggaatt cgtgcagcct ccgggggcga
     3241 tacgcgtgtc gcgtccgccg ccgtgttcgc aatggcactt ggctcgccgc cgcgttcgcg
     3301 gcgtccgagc tccaggtacg ccgtcaagag caaaagcacg ggcgcggcaa atccgatgag
     3361 gaagggtgcg aattcgatgc tgcgcgccca ccacccctgc tcggtggtga cgagggtcca
     3421 gagcctcgcg cccagaggca atacggtgaa caccattgtc gggacgacga accaggcgct
     3481 gttgccttcg ccgaggcccg ccatgcgggc gctgaaaatg gagaccgcgc ccaccagccc
     3541 cgccgacacg attgcagcgg gcaccccgag cgctgcgtac gcccaactgc cgacggcatg
     3601 gagcacgagc agcacggtcg ccagcatgac gagtcgtctc agccaggcat cgctcatctt
     3661 cgcagtctgg ccgggcgcgg tgaccggcgt ctgtgcgcgc tcgcacattg catggccgct
     3721 cagcgcgtgc tcgaacgcca tggacgtcgc agatgccctt gccgatgaac gaccccacgg
     3781 tcggagacga cactgtcgcc gtcgcgagac taccgcctaa gcacgctgtc gcacagacgc
     3841 caaggcactc gcggcgtagc gtagtcacat gcgccgcatc cagcgccgcg ccgccgagat
     3901 ttcttaatcg ctacgttaca ggagatcaat catgactatg ctcaaacgtt ttaccgcagt
     3961 tttcatcgct gcactgatgc tctcggcgct cggttgcgcg acgcagtccg agccgcaaag
     4021 ccccggcgcc tacatggacg acagctggat caccaccaa
//
LOCUS       DASUNT010000078         3805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_624250, whole genome shotgun sequence.
ACCESSION   DASUNT010000078 DASUNT010000000
VERSION     DASUNT010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624250"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..118
                     /locus_tag="VFX94_01620"
     CDS             <1..118
                     /locus_tag="VFX94_01620"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5766906.1"
                     /translation="DRRPARTCVGVTALARGARVEIDFIARRIQERRRRPRL"
     gene            190..870
                     /locus_tag="VFX94_01625"
     CDS             190..870
                     /locus_tag="VFX94_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5766907.1"
                     /translation="MFWVVAIAAMLLALNWIYQVVRKPAELFFPVSGVLNKTPEETWR
                     QYEPIFRRHSTALMTPELLAALAQVEGAGNPVALTPWRVRASTDPREIYRPASSAVGM
                     YQITDGTFRQARRYCIRDHVAVEDCWLNSLYMRVIPSHAVELTSAYLDRRVAALLEAR
                     RVAATHEQKQHLAAFMHLCGAGAGEAYARAGFKLAPGQRCGEHDPRLYLARVAAMKQR
                     FARLSAIR"
     gene            883..1104
                     /locus_tag="VFX94_01630"
     CDS             883..1104
                     /locus_tag="VFX94_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766908.1"
                     /translation="MHPALNGFLVGVGIAAFLIVIEYMMVKKAVEERATLTHRKPQFE
                     PTDKARIRAVINFCLFVPPGFAVAFWLLD"
     gene            complement(1057..1413)
                     /locus_tag="VFX94_01635"
     CDS             complement(1057..1413)
                     /locus_tag="VFX94_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766909.1"
                     /translation="MRGLGAIAVMALAAWTFLSAPSGGWAFAAAELAFLAWLARVLTK
                     EPARAELLRESASTLAATGLASLVLVPWLTYKLQWPQAILIGAMLFAVGALTKRAVRR
                     LSVEQPERHGEAGRNE"
     gene            1441..1974
                     /locus_tag="VFX94_01640"
     CDS             1441..1974
                     /locus_tag="VFX94_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HEX5766910.1"
                     /translation="MPCAALERHPDAACEALRGVAASVTRNAGGGLEVAFVLDGEIER
                     LRIRAPRTARMAQELWRHTCCEIFIARRGAPAYREFNLSPSREWAAYEFTRYREGRLL
                     RDEALAPGIVVRTAPGQLHLRACIPVADAQELAIGLAAVVEEKNGALSYWALRHAPGK
                     PDFHHPDAFALTLAALP"
     gene            1971..3164
                     /locus_tag="VFX94_01645"
     CDS             1971..3164
                     /locus_tag="VFX94_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEX5766911.1"
                     /translation="MKLGIERLLEERKLADPLAGRRVALLAHPASVTRELTHSLDALV
                     AAGVRLTAAFGPQHGLRGDKQDNMVESADFTDPVHGIPVFSLYGTVRRPTDEMMQAFD
                     VLLVDLQDLGCRVYTFLTTLRYLLEAAARHAKSVWVLDRPNPVGRPVEGLRLRAGWES
                     FVGAGALPMRHGLTLGEAGSWFVRALKLDVDYHVVEMQGWKPTEGWPLGSRVWVNPSP
                     NAPNLFMARCYPGTVMLEGSTLSEGRGTTRPLEILGAPGLDSKSLLEKMHSLAPEWLG
                     GSRLRPCWFEPTFHKHAGKLCAGLQIHAEAPHYAAQAFKPWRLVALAFKALRSLAPGY
                     ALWRDFPYEYDTRLAIDVINGGTLLRQWVDDPAANPEDLDAAARADEDAWREEREAVL
                     IYRSG"
     gene            complement(3166..3357)
                     /locus_tag="VFX94_01650"
     CDS             complement(3166..3357)
                     /locus_tag="VFX94_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766912.1"
                     /translation="MAQKHDRRADDISADEVKQFWHDFCERRGVDAATRARGDARIDD
                     DPEYWADQTMPRLLDAVAT"
     gene            complement(3445..>3805)
                     /locus_tag="VFX94_01655"
     CDS             complement(3445..>3805)
                     /locus_tag="VFX94_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5766913.1"
                     /translation="SQRGSCDGEVVDVVCTGRFFDFFEKRAGKWAIVRRQPIYEKDRM
                     DPVDPSARIKLDPDLLGSIPEGYRHLGYLQSKLGFAIKRDLPQLKGDAVARLYRHGRA
                     WLDGAPSPFPNGEGPAP"
BASE COUNT          587 a         1320 c         1288 g          610 t
ORIGIN      
        1 cgacaggcgg cccgcgcgca cttgcgtagg cgtcaccgcg ctcgcccgcg gcgcgcgcgt
       61 ggagatcgac ttcatcgcgc gccgcattca ggagcgccgc cggagacccc gcctatagcg
      121 cgccgcacgc gctggaggct ggggcggcgt ttcgccgccg tcttcccgcc cggatcgcgg
      181 gagaggaagg tgttctgggt cgtcgcgatc gctgcgatgc tgcttgcgct caattggatc
      241 tatcaggtgg tgcgcaagcc ggccgagctt ttctttccgg tgagcggggt gctgaacaag
      301 acgccggagg aaacctggcg gcagtacgag ccgattttcc ggcgtcactc cactgcgctg
      361 atgaccccgg agctgctcgc ggcgctagcg caggtggaag gcgcgggaaa cccggtggcg
      421 ctcacgccgt ggcgcgtgcg cgcgagcacc gacccgcgcg agatctaccg tccggcctcg
      481 agcgcggtcg gcatgtacca gattaccgac ggcacctttc ggcaggcgag gcgctactgc
      541 atccgcgacc acgtggcggt cgaggactgc tggctcaaca gtctctacat gcgcgtgatc
      601 ccgagccacg cggtcgagct cacctccgcg tatcttgacc ggcgcgtcgc cgccttgctc
      661 gaggcgcggc gcgtcgccgc cacgcacgag cagaagcagc atctcgccgc cttcatgcac
      721 ctttgcggag ccggcgcggg cgaggcttat gcgcgcgccg gcttcaagct cgcgccgggg
      781 cagcgctgcg gcgagcacga cccgcggctt tatctcgccc gggtcgcggc gatgaagcag
      841 cggtttgccc ggttatcggc aatccgctag agtaaatcgt caatgcaccc cgcgctcaat
      901 ggcttccttg tcggtgttgg catcgctgcg ttcctgatcg tcatcgaata catgatggta
      961 aaaaaggcgg tggaggagcg cgcgacgctg acgcaccgca agccccagtt cgagcccacc
     1021 gacaaggcgc gcatccgcgc cgtcatcaac ttctgcctat tcgttccgcc cggcttcgcc
     1081 gtggcgttct ggctgctcga ctgagagccg acgcaccgcg cgcttcgtga gcgcgccgac
     1141 cgcgaacagc attgccccga tcaggatcgc ctgcggccat tggagcttgt aagtgagcca
     1201 cggcaccagc acgaggctcg caaggccggt agcggcgagc gtcgaagcgc tttcgcgtag
     1261 aagctccgcg cgagccggct ccttcgtcag cacgcgcgca agccaggcca ggaaagcgag
     1321 ctccgccgcg gcgaacgccc agccgccgct cggcgcagag agaaacgtcc aggccgctaa
     1381 cgccatgacc gcgattgcgc cgagccccct catcgcctgc atgataggct cggcgccgcc
     1441 atgccgtgcg ccgcgctcga gcgccatccc gacgccgcct gcgaggcgct tcgcggcgtc
     1501 gcggcgagcg tgacgcgcaa cgccggcggc ggcctggagg tcgcattcgt gctcgacggc
     1561 gagatcgagc gcctgcgcat ccgggcgcct cgaaccgcgc gcatggcgca ggagctgtgg
     1621 cgccacacat gctgcgaaat cttcatcgcc cgccgaggcg cgccggccta ccgggaattc
     1681 aatctctcgc cctcgcggga gtgggcggcg tacgagttca cgcgctaccg cgaagggcgt
     1741 ctgctgcgtg acgaagccct ggcgccggga atcgtggtgc gcaccgcgcc cgggcagctc
     1801 catctacgcg catgcatccc cgtggccgat gcgcaagagc tcgctatcgg ccttgcggcc
     1861 gtagtcgaag aaaagaacgg tgcgctctcc tactgggcac tcaggcatgc gcccggcaag
     1921 ccggatttcc accacccgga cgcgttcgcg ctcacgctgg cggcgctgcc atgaagctcg
     1981 gcatcgagcg gctgctcgaa gagcgcaagc ttgcagatcc gctcgcgggg cggcgcgttg
     2041 cgctgctcgc gcatccggcg tcggtaacgc gcgagctcac ccattcgctc gacgcgctcg
     2101 tggccgccgg cgtcaggctc acggccgcgt tcggtccgca gcacgggctg cgcggcgaca
     2161 aacaggacaa catggtggag tcggccgact tcaccgatcc ggtgcacggc atcccggtat
     2221 tcagccttta cgggacggtg cgccggccga ccgacgagat gatgcaggcc ttcgacgtcc
     2281 tgctcgtcga tctgcaggac ctgggctgcc gcgtttacac attcctcacc acgctgcgct
     2341 acctgctcga ggcggcggcg cggcacgcaa agagcgtctg ggtgctcgac cgcccgaacc
     2401 cggtaggccg tccggtggaa ggcctcaggc tgcgcgcggg ctgggaaagc ttcgtcggcg
     2461 ccggcgcgct accgatgcgc cacggcctca cgctcggcga ggccggaagc tggtttgtcc
     2521 gtgcgctcaa gctcgacgtg gattaccacg tcgtcgagat gcaagggtgg aagccgaccg
     2581 agggctggcc gctcggcagc cgcgtctggg tcaatccgag ccccaacgcg ccgaatcttt
     2641 tcatggcgcg ctgctacccc ggcacggtga tgctcgaagg cagcacgctg tccgaagggc
     2701 gcggcaccac gcggccgctg gagatcctgg gcgctcccgg cctggactcg aagagtctcc
     2761 ttgagaagat gcattcgctt gcgccggaat ggctcggcgg ctcccggctg cggccgtgct
     2821 ggttcgagcc gactttccac aagcacgccg gaaagctgtg cgccgggctg cagatccacg
     2881 ccgaagcgcc gcactacgct gcgcaggcgt tcaagccttg gcggctggtc gcgcttgcgt
     2941 tcaaagcgct gcgcagcctg gcgcccgggt acgccctctg gcgggacttt ccctacgagt
     3001 acgacacgcg gcttgcgatc gacgtgatca atggcggaac gctgctgcgc cagtgggtgg
     3061 acgaccctgc cgccaatcca gaggacctcg acgccgctgc acgcgccgac gaggacgcct
     3121 ggcgagagga gcgcgaggcg gtgctgatct acaggtcggg ctagtctacg ttgcgacggc
     3181 gtcgagaagg cggggcatcg tctggtccgc ccaatactcg ggatcgtcgt cgatcctggc
     3241 atcgccgcgc gcacgcgtcg ccgcatcgac gccgcggcgt tcgcagaagt cgtgccagaa
     3301 ctgcttcacc tcgtcggcgc ttatgtcgtc ggcgcgccta tcgtgctttt gagccattct
     3361 gggccccgat attagggcat tttccggccc gcatttgcag ccgccgtgtt gcggcggctc
     3421 ccgcgccggc cgagtccgct cggactaggg agccggacct tccccgttgg gaaaaggcga
     3481 tggcgcgccg tcgagccagg cccgcccgtg gcgataaagg cgagccactg catcgccctt
     3541 gagctgcggc aggtcccgct ttatcgcgaa cccaagcttc gactgcaggt accccaggtg
     3601 gcggtagccc tcggggatcg agccgagcag gtcgggatcg agcttgatcc gcgcggaggg
     3661 gtccacgggg tccatgcgat ccttctcata gatcggctgg cgccgcacga tcgcccactt
     3721 gccggcgcgc ttctcgaaga aatcgaagaa ccggccggtg cacaccacgt cgaccacctc
     3781 accgtcgcag ctcccgcgct ggctg
//
LOCUS       DASUNT010000079         2386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_799689, whole genome shotgun sequence.
ACCESSION   DASUNT010000079 DASUNT010000000
VERSION     DASUNT010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799689"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /gene="mreD"
                     /locus_tag="VFX94_01660"
     CDS             <1..279
                     /gene="mreD"
                     /locus_tag="VFX94_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX5766914.1"
                     /translation="LGQHALAYSLVAFLGIWLSRRILWFGPALQALHVGAMLLVAQGA
                     SVLVRLAAGDDFPGWQVFIGPFAGAFLWPLTSWLLLLPQRRPAKEQTI"
     gene            276..2192
                     /gene="mrdA"
                     /locus_tag="VFX94_01665"
     CDS             276..2192
                     /gene="mrdA"
                     /locus_tag="VFX94_01665"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX5766915.1"
                     /translation="MMGAAELRNSERELQQFQLRIAIAGIAMLAAFGVLLARFVFLQV
                     VQHDHYRAKAEDNRISIVPIAPNRGLIIDRNGVVVARNYSGYTLEIFPRRVRSVEETI
                     DQVSELIEITPKDRARFKRLAVETRNPESLPIRTRLSDEEVAKFAANRFRYEGVEIKA
                     RLFRQYPFGEIGSHVIGYMGRINQADQAALEQEGVDANYRGTDYIGKSGVEATYEREL
                     HGTTGFEQVEIDAAGRGIRTLSHTPAVSGQNVVLTLDMWLQRVAENAFGSRRGALVAI
                     EPGSGAVLALVSKPGFDLNLFVDGIDPQAWVELNNSPDRPLNNRAIAGVYPPGSTFKP
                     FMALAALELGKRRPSSTINDPGYFVFGERRFRDSKPGGHGVVDLYKSIVVSSDTYYYM
                     LANDMGIDAIAGFMKQLGLGSPTGIDLQGEAAGVLPSPEWKMKRFRRPEQQRWYPGET
                     ISIGIGQGYNAYTPIQLAHAMATVAANGHMYRPRVVGHIENPRTRERRSFEPEQTAQV
                     PLRREWVDFVKRAMAGVNKEGTGARAFAKAEYSSGGKTGTAQVIGMKQGEKYEEAKIA
                     ERHRDHSLFIAFAPLENPIIALAVIVENGGFGARAAAPISCTVLDYYLLGKLPPGLQE
                     PTPEDEFDDGKESD"
     gene            2192..>2386
                     /locus_tag="VFX94_01670"
     CDS             2192..>2386
                     /locus_tag="VFX94_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766916.1"
                     /translation="MEIARVPVQRIALRLVEGIDSTLLIIIASLSMLGFAALFSASYD
                     APGRVFSQLVSLCIALVAMWV"
BASE COUNT          408 a          810 c          784 g          384 t
ORIGIN      
        1 ctcggacagc acgcgctcgc ctattcgctc gttgcgttcc tcggcatctg gctgtcgcgc
       61 aggatcctgt ggttcggccc ggcgctgcag gcgctgcacg tcggcgcgat gctgctcgta
      121 gcgcagggcg cctcggtgct ggtgcggctc gccgccggcg acgactttcc aggctggcag
      181 gtgttcatcg gcccgttcgc cggcgcgttc ctctggccgc tgacgagctg gctgctgctg
      241 cttccacagc gccgcccggc gaaggagcag accatatgat gggcgccgcc gagctgcgca
      301 actccgagcg cgagctgcag cagttccagc tgcgcattgc gatcgccggc atcgcgatgc
      361 tcgcggcttt cggggtactc cttgcgcgct tcgttttcct gcaggtcgtg cagcacgacc
      421 actaccgcgc gaaggccgag gacaaccgta tctcgatcgt tccgatcgcg cccaaccgcg
      481 gactgatcat cgaccgcaac ggcgtcgtgg tggcgcgcaa ctattcgggc tacacgctcg
      541 agatctttcc gcgccgcgtg agaagcgtcg aggagacgat cgaccaggtg tcggagctga
      601 tcgagatcac gccgaaggac cgagcgcgct tcaagaggct cgccgtggaa acccgcaacc
      661 ccgagagcct gccgatccgc acgcgcttga gcgacgagga ggtggcgaaa ttcgccgcca
      721 accgcttccg ctacgagggc gtggagatca aggcgaggct cttccggcag tatccgttcg
      781 gcgagattgg ctcgcatgtc atcggctaca tgggacgcat caaccaggcc gaccaggcag
      841 cgctcgagca ggaaggcgta gacgccaact atcgcggcac cgattacatc ggcaagagcg
      901 gcgtcgaagc gacctacgag cgcgagctcc acggcaccac cgggttcgag caggtggaga
      961 tcgatgccgc gggtcgcggc atccgcacgc tttcacacac gccggcggtg tccggtcaga
     1021 acgtcgtgct gacgctcgac atgtggctgc aacgcgtagc cgagaacgcc ttcggcagcc
     1081 gccgcggcgc gctggtcgcc atcgaaccgg gcagcggtgc cgtgcttgcg ctggtatcca
     1141 agccgggctt cgacctgaat cttttcgtcg acggcatcga cccgcaggcc tgggtcgagc
     1201 tcaacaactc cccggaccgg ccgctcaata accgcgccat cgccggggtg tatccgcccg
     1261 gctccacctt caagccgttc atggcgctgg cggcgctgga actcggcaag cgcaggccct
     1321 cgagcacgat caacgacccg ggctacttcg tattcggcga gcggcgcttc cgcgactcga
     1381 agcccggcgg ccacggggta gtggacctct acaagtcgat cgtcgtgtcc tccgacacgt
     1441 actactacat gctcgccaac gacatgggga tcgatgcgat cgccgggttc atgaagcagc
     1501 tcggcctcgg cagcccgacc ggcatcgacc tgcagggcga agcggcaggc gtgctgcctt
     1561 cgcctgagtg gaagatgaaa cgtttccgcc gacccgagca acagcgctgg tatcccggcg
     1621 aaacgatctc catcggcatc ggacagggct acaacgccta cacgccgatc cagctcgcgc
     1681 atgcaatggc caccgtcgcc gccaacgggc acatgtaccg gccgcgcgtc gtcggccaca
     1741 tcgagaatcc gcgcacgcgc gagcgccgca gcttcgagcc ggagcagacc gcgcaggtac
     1801 cgctcaggcg cgaatgggtg gatttcgtca agcgcgccat ggcgggcgtc aacaaggagg
     1861 gcaccggcgc gcgcgccttc gccaaggccg agtactcgag cggcggcaag acgggcaccg
     1921 cgcaggtgat cggcatgaag cagggcgaga aatacgagga ggcgaagatc gccgagcgcc
     1981 accgcgacca ttcgctgttc atcgcgttcg cgccgctcga gaacccgatc atcgcgctcg
     2041 ccgtcatcgt ggagaacggc ggcttcggcg cgcgcgcggc cgcgccgatc tcgtgcacgg
     2101 tgctcgatta ttacctcctc ggcaagctgc cgcccgggct gcaggagccc acgcccgagg
     2161 acgagttcga cgacggcaag gaaagcgact gatggagatc gcgcgcgtcc cggttcagcg
     2221 catcgcgctg cggctcgtcg agggaatcga cagcacgctt ctcatcatca tcgcgtcgct
     2281 ttccatgctg ggcttcgcgg cgctgttctc cgcgagctac gacgcgcccg gccgggtgtt
     2341 cagccagctg gtcagcctgt gcattgcgct cgttgccatg tgggtc
//
LOCUS       DASUNT010000080         3119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_887347, whole genome shotgun sequence.
ACCESSION   DASUNT010000080 DASUNT010000000
VERSION     DASUNT010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887347"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..277
                     /locus_tag="VFX94_01675"
     CDS             68..277
                     /locus_tag="VFX94_01675"
                     /inference="COORDINATES: protein motif:HMM:NF033216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HEX5766917.1"
                     /translation="MRTILVALFAFAALAGCSSDYILSTKTGAMLESSGKPYLNERTG
                     MYEYRTKDGKDAWIRKEEVVEILER"
     gene            453..2300
                     /gene="htpG"
                     /locus_tag="VFX94_01680"
     CDS             453..2300
                     /gene="htpG"
                     /locus_tag="VFX94_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEX5766918.1"
                     /translation="MPHTPARETLGFQAEVKQLLDLMIHSLYGNKEIFLRELISNASD
                     AADKLRFEALANNALYESDAELKVRIAVDKHARTLTVSDNGIGMSRDEVVQNIGTIAK
                     SGTREFFQSLTGDQAKDARLIGQFGVGFYSSFIVADRVTLLTRRAAGEAVRWECDMSR
                     GAAEYTIEPAEKAGRGTDVILHLREGEDELLDEWRLKQIIRKYSDHIALPIMMGSETV
                     NQASALWTRPKTEITEEQYIEFYKHVAHDFEPPLAWTHNRVEGRHEYVQLLYLPGRAP
                     FDLWDRTQRHGLKLYVRRVFIMDDAQQLLPGYLRFVRGVVDSSDLPLNVSREILQQSR
                     DVAAIRAGCTRRVLDLLEELAAGQPEKHARLWGEFGRVLKEGVGEDTANRDRIAKLLR
                     FASTHTDSEAQTVSLADYVGRMKPRQDTIYYVTAETFLAAKNSPHLEIFRSRGIEVLL
                     LYDRIDEWLVANLTEHDGKRLASVARGELDLSSIQSESKEPDWKAGEHAELLRALKGA
                     LGERVKDVRLSRRLTESPSCLVADEHDLGGNLARILRAAGQKVPELRPILELNPAHPI
                     VLRLKPADDRFADWAALLYEQALLAEGGSLHDPAGYVKRANSLLLGLAK"
     gene            complement(2346..2729)
                     /locus_tag="VFX94_01685"
                     /pseudo
     CDS             complement(2346..2729)
                     /locus_tag="VFX94_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161500.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(2874..>3119)
                     /locus_tag="VFX94_01690"
     CDS             complement(2874..>3119)
                     /locus_tag="VFX94_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766919.1"
                     /translation="LAFGCAILFCGDKVVRPLAAREGTHLGFAWVLMGCLGGFQALGL
                     VGVVIGPVVLTLARELWAECMRRPDEAPAPVAFVRRA"
BASE COUNT          573 a         1047 c         1008 g          491 t
ORIGIN      
        1 gtcggcgtca ggccggaggc gaagaagtga ctcgcgcagt cgaaaccccg cagcatggag
       61 aatcgtcatg agaaccatcc tggttgcact cttcgcattc gcggcgctcg ccggctgcag
      121 cagcgactac atcctgagca ccaagaccgg agcgatgctc gaatcgagcg gcaagccgta
      181 cctcaacgag cggaccggca tgtacgagta tcggaccaag gacggcaagg acgcctggat
      241 ccgcaaggaa gaggtggtgg agatccttga gcgctgagcc agcgatcagc ggctccttcg
      301 tgccgctatg agcccgaggt cgcttgctcg aactccgtag caagcgcgta gagcgggaac
      361 ggcgctcgtc gggcggcatt cctatggccg gccgggcttg aaaagccgcg cccgagcgcc
      421 taatttcgcc ggaagaacgg agaccctttt tcatgccgca tacccctgcc cgcgagacgc
      481 taggcttcca ggccgaggtg aagcagctcc tggatctcat gatccactcg ctctacggca
      541 acaaggaaat cttcctgcgc gagctgatct ccaacgcctc ggatgcggcc gacaagctgc
      601 gcttcgaggc gctcgccaac aacgcgcttt acgagtcgga cgccgagctg aaggtgcgca
      661 tcgcggtcga caagcatgcg cgcaccctca ccgtgtcgga caacggcatc ggcatgtcgc
      721 gcgacgaggt ggtgcagaac atcggcacga tagcgaagtc cggcacgcga gagttcttcc
      781 agtcgctcac cggcgatcag gcgaaggacg ctcggctgat cggccagttc ggcgtgggct
      841 tctactcatc gttcatcgtc gccgatcggg tgacgctcct cacgcggcgc gcggcgggcg
      901 aggcggtgcg ctgggaatgc gacatgagcc gaggcgcggc cgaatacacc atcgagcccg
      961 cggagaaggc cgggcgcggc accgacgtga tcctgcacct gcgcgaaggc gaggacgagc
     1021 tcctcgacga gtggcggctg aagcagatca tccgcaagta ctccgaccat atcgctctgc
     1081 cgatcatgat gggatcggag acggtcaacc aggcgagcgc gctgtggacg cggccgaaga
     1141 ccgagatcac cgaggagcag tacatcgagt tctacaagca cgtcgcgcac gacttcgagc
     1201 cgccgctcgc ctggacgcac aaccgcgtcg aagggcggca cgaatacgtg cagcttctct
     1261 accttcccgg gcgcgcgccg ttcgatctgt gggaccgcac gcagcgccac gggctaaagc
     1321 tctacgtgcg ccgcgtattc atcatggacg acgcccagca gctcctgccc gggtacctgc
     1381 gcttcgtccg cggcgtggtg gattcgagcg acctgccgct caacgtctcg cgcgagatcc
     1441 tgcagcaaag ccgggatgtc gccgcgattc gcgccggctg caccaggcgc gtgctcgacc
     1501 tgctcgagga gcttgccgcc ggccagcccg agaaacatgc ccggctctgg ggcgagttcg
     1561 gccgcgtgct caaggagggc gtcggcgagg acacggcgaa tcgcgaccgc atcgcgaagc
     1621 tgctgcgctt cgcatcgacg catacggaca gcgaggcgca aaccgtttcg cttgccgact
     1681 acgtcggccg catgaagccg cgccaggaca cgatctacta cgtcaccgcc gagacctttc
     1741 tcgcggcgaa gaacagcccg cacctcgaga tcttccgcag ccgcggcatc gaggtgctgc
     1801 tgctctacga ccgcatcgac gagtggctgg tggcgaatct cactgagcac gacggcaagc
     1861 ggctcgcctc ggtggcacgc ggcgagctcg acctctcctc catccagtcc gaatcgaagg
     1921 agcccgactg gaaagcgggc gagcacgccg agcttttgcg cgcgctgaag ggtgcgctcg
     1981 gcgaacgagt gaaggacgtc cgcctgagcc ggcgcctcac cgaatcgccc tcctgcctcg
     2041 ttgccgacga gcacgacctt ggcggcaacc tcgcgcgcat cctgcgcgcg gcggggcaga
     2101 aagtgcccga gctgcggccg atcctcgagc tgaacccggc gcatccgatc gtcctgcgcc
     2161 tgaagccggc cgacgatcgc ttcgcggact gggcggcgct gctttacgag caggcgctgc
     2221 ttgccgaagg cggcagcctc cacgaccccg cggggtatgt gaagcgcgct aactcgctct
     2281 tgctagggct tgcaaagtga agacgtagcg aaaggggcgc gcgcaagcgg ccgcccggtc
     2341 tctccttatt tcttgaccag cgggcagcgg ctcgcgctca ggggctgaaa cgcctcctcg
     2401 ccggggacaa cggccttgat atggtagtag tcccaaggcg actttgattc cgccggcttt
     2461 ttgacctgca ccacgtacat gtcgtggacc atgcggccat cggcgcggat cgttcccttc
     2521 ttcgcgaaca tatcctggat gggcagctct ctcatcttct tcatcacggc atccgggtca
     2581 tcggtgccgg cggcgtgcac tgccttcagg taggtcatcg tggccgaata gttgccggcg
     2641 tgcagcatgg tcggcatctt ctttgccttc tcgaagaagc ggtggctcca ggcgcgcgtc
     2701 tcgtcgttca ggtcccagta gaagccctcc catctgcacg ctaaggactg acccggagcc
     2761 gcccagatcg gagtagagcc cggacatgtc cgagagcacg ccgatcttcg ccatgccatc
     2821 ggagagcttg ggtgactgcg cccaggctgc gcccgcgccg agcatagcgg cgctcacgca
     2881 cgcctgacga acgcaaccgg cgcgggcgct tcatcgggcc tgcgcatgca ctcggcccag
     2941 agctctcgcg cgagcgtcag caccaccgga ccgatgacca ccccgaccag gccaagcgcc
     3001 tggaagccgc cgaggcagcc catgagcacc caggcgaatc cgaggtgcgt cccctcgcgc
     3061 gcggcaagcg gccgcacgac cttgtcgccg cagaagagga tcgcgcagcc gaacgcgag
//
LOCUS       DASUNT010000081         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_799794, whole genome shotgun sequence.
ACCESSION   DASUNT010000081 DASUNT010000000
VERSION     DASUNT010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799794"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VFX94_01695"
     CDS             <1..764
                     /locus_tag="VFX94_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ni,Fe-hydrogenase III large subunit"
                     /protein_id="HEX5766920.1"
                     /translation="NHLGDLGYLGNDVALAFGFFQFWRLKETLLRAHREAFGHRYLMD
                     FVIPGGVAHEPKNMRRLLEVLNRIKTEVALLRGIYDEHAGVQDRFIMAGQVLPELAAR
                     LGLTGPAARASGQAHDLRSDVPIPPYESLAVRKAGEKRGDVAARVTVRFDELAESLRL
                     IEELAERLPAGPTEAPWKAPRTGSRGCGWIEGWRGEVFIALEVGPEGGLERVHAHDPS
                     WQNWPALERAVLGNIVPDFPLINKSFNLSYSGHDL"
     gene            768..1277
                     /locus_tag="VFX94_01700"
     CDS             768..1277
                     /locus_tag="VFX94_01700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX5766921.1"
                     /translation="MYTLLKQIARTGIVTEPAPRADRALRELEQRLSETVLKHFGRSL
                     AIRHVDAGSCNGCELEIHACNSPYYNLERMGMRFVASPRHADLLLVTGPVSRNMETAL
                     RRTYDATPEPKLVVAIGDCGCTGGIFGESYASCGRVSNVIPVDVAVPGCPPEPIAIIR
                     GILAAVEAR"
     gene            complement(1289..1681)
                     /locus_tag="VFX94_01705"
     CDS             complement(1289..1681)
                     /locus_tag="VFX94_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766922.1"
                     /translation="MRAALLVIAGLALAGYVAVAWILPSMAGSEVRAAAQALVAGAEP
                     AKQQVAARAATSGNLSGAGNGVKLVEKDDPKYGRMRWIVTDNGAIRAWNEQNALEVTF
                     TPSFQGGKTSWSCKGYPVDAMPPGCGKS"
     gene            1813..2091
                     /locus_tag="VFX94_01710"
     CDS             1813..2091
                     /locus_tag="VFX94_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX5766923.1"
                     /translation="MPSVRLKISGLVQGVGFRYAMRSEASRRGLSGWVRNRRDGGVEA
                     LVQGEAAAVESLVEWARRGPPGARVDALRSEPADGEPAAAGFDLRPTV"
     gene            complement(2088..>2345)
                     /locus_tag="VFX94_01715"
     CDS             complement(2088..>2345)
                     /locus_tag="VFX94_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766924.1"
                     /translation="RAMAEFLSGRFDGPLEVLRFESRELSAESAATLKRKLDELVAQF
                     NECAEADSSLPAGRRVGVALLAGCRPWQFSAVNALKRRKSA"
BASE COUNT          374 a          805 c          781 g          385 t
ORIGIN      
        1 cgaaccatct cggggacctc ggctacctcg gcaacgacgt cgcgctcgcc ttcggcttct
       61 tccagttctg gaggctgaaa gagacgctcc tgcgcgcgca tcgggaagca ttcggccacc
      121 gttatttgat ggactttgta ataccgggcg gtgtcgcaca cgagccgaaa aacatgaggc
      181 gcctattgga agtactcaat cgcataaaga cggaggtcgc gctgcttcgc ggcatctatg
      241 acgagcacgc gggcgtccag gaccgtttca tcatggcggg tcaggttctt cccgagcttg
      301 ccgcccgcct cggccttacc gggccggcag ctcgggcgag cggtcaggct cacgacctgc
      361 gctccgacgt ccccattccg ccttacgaaa gcctcgccgt gcgcaaagcc ggcgagaagc
      421 ggggggatgt cgccgcgcga gtgacagtgc gcttcgacga gctcgccgaa tcgctgcggc
      481 tcatcgaaga gctcgccgag cggctgcccg ccggaccgac cgaggcgcca tggaaagcgc
      541 cccggaccgg cagtcgcggc tgcggctgga tcgagggctg gcgcggtgag gtgttcatcg
      601 cgctcgaagt gggcccggag ggcggcctcg agcgggtcca cgcccacgat ccgtcgtggc
      661 agaactggcc tgcgctcgag cgcgcggtac ttggcaacat cgtcccggac ttcccgctca
      721 ttaacaagtc cttcaatctg agctacagcg gccacgacct ctaagccatg tataccctcc
      781 tcaagcagat cgcgcgcaca ggcatcgtga ccgagcctgc gccgcgcgcc gaccgcgcgt
      841 tgcgcgagct cgagcagcgt ctgagcgaaa cggtgctcaa gcatttcggc cgctcgctcg
      901 ccatccgcca cgtcgacgcg ggatcgtgca acggctgcga gctcgagatc cacgcctgca
      961 acagccccta ctacaacctc gagcgtatgg gcatgcgatt cgtggcgagc ccgcggcatg
     1021 ccgacctgct gctcgtcact ggcccggtat cgcgaaacat ggagaccgcc ttgcggcgca
     1081 cttacgacgc gaccccggag ccgaagctcg tagtggcgat cggcgactgc ggctgcaccg
     1141 gcggcatatt cggggaaagc tacgcgagct gcggccgggt gtcgaacgtg atcccggtgg
     1201 acgtggccgt tcccggctgc ccgcccgagc cgattgcgat catccgcgga atcctggcgg
     1261 cggtagaagc caggtaacct gcggcgtcct agctcttccc gcagccgggc ggcatggcgt
     1321 cgactgggta acccttgcag ctccagctcg tcttgccgcc ttggaacgac ggcgtgaacg
     1381 tcacctcgag cgcgttctgc tcgttccagg cgcgaatggc gccgttgtcg gtcacgatcc
     1441 acctcatcct gccgtacttg ggatcgtcct tctccacgag cttgacgccg ttgcccgcgc
     1501 cggacagatt gcctgatgtc gccgccctcg cggcgacctg ctgcttcgcc ggctccgccc
     1561 ccgccaccag cgcttgcgct gcggctctca cttcggagcc ggccatcgaa ggtaggatcc
     1621 acgccaccgc cacgtatccg gccagggcga gaccggcgat cactagcaac gcagcacgca
     1681 tttcgttccc ctttggtgct ctcccgagct tttttgtact tctcagaaca gcttaccatg
     1741 gagcgttaca acgagctttg cgcggcccgc ggggcgtgtg acgcggcgca ggccgcctga
     1801 ccttctttaa ccatgccgag cgtgcggctg aagatcagcg ggcttgtgca gggcgtcgga
     1861 ttccgctacg ccatgcgcag cgaggcctcg cgacgcggcc tgagcggctg ggtgcgcaac
     1921 cggcgcgacg gcggcgtgga agccctggtg caaggagagg cggcggccgt cgagagcctg
     1981 gtcgagtggg cgcggcgcgg gccgccgggc gcgcgagtcg acgctttgcg ctccgagccg
     2041 gcagacggcg agccggcggc cgccgggttc gacctgcggc cgacggtcta ggcgctcttg
     2101 cggcgcttga gcgcgttcac ggcagagaat tgccacgggc ggcagccggc gagcagggcc
     2161 acgcccacgc gccggcccgc cggcagggag gaatcggcct cggcgcactc gttgaactgc
     2221 gccacgagct cgtccagctt gcgcttgagc gttgccgccg attccgcgga gagctcgcgc
     2281 gactcgaagc gcagcacctc gagcgggccg tcgaagcgac cggaaagaaa ttcagccatc
     2341 gcgcg
//
LOCUS       DASUNT010000082         2640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_10717, whole genome shotgun sequence.
ACCESSION   DASUNT010000082 DASUNT010000000
VERSION     DASUNT010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10717"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2113)
                     /gene="uvrA"
                     /locus_tag="VFX94_01720"
     CDS             complement(<1..2113)
                     /gene="uvrA"
                     /locus_tag="VFX94_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX5766925.1"
                     /translation="MDLIRVRGARTHNLKNLSVELPRNRLVVITGLSGSGKSSFAFDT
                     LYAEGQRRYVESLSAYARQFLELMAKPEVDLIEGLSPAIAIEQKAASHNPRSTVGTVT
                     EVHDYLRLLFARAGTPYCPQHGLALEAQTVSEMVDQVAALPEATRVMILAPVVAGRKG
                     EQADLLGELRAQGFTRVRIDGAVYELDATPRLAKNVKHSVDVVVDRLRVRADARQRIA
                     ESLETALRHADGKVVVIELETGAEHLFSARYSCPVCDYAVPELEPRLFSFNNPAGACP
                     RCDGLGTVEFFDPKRIIAHPNLSLAGGAIRGWDRRNAFYFSMLQSLARHYGFDIESPW
                     EMLDERIQKLILYGSGSETIAFRYPSERGRAAVKEHAFEGVIPNHERRYRETDSQVVR
                     EELAKLRNLRACPECGGARLRREARHVRVADTTLQEISAWPLKQASEFFRTLTLKGAK
                     AQVAERIVREIASRLEFLMNVGLEYLSLERSAETLSGGEAQRIRLASQIGSGLTGVMY
                     VLDEPSIGLHQRDNARLIATLKQLRDLGNSVIVVEHDEEAIRAADYVIDMGPGAGEAG
                     GRIVAQGSPAEVISNPQSLTGRYLAREAAIAVPPHRRRAKQTLVLQGARGNNLKDIDV
                     ELPLGVLVCVTGVSGSGKSTLVNDTLYAALARALHGASAEPAPHGALAGMEHIDKVIA
                     VDQSPIGRTPRSNPATYTGLFA"
     gene            2191..>2640
                     /locus_tag="VFX94_01725"
     CDS             2191..>2640
                     /locus_tag="VFX94_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5766926.1"
                     /translation="MSRAELRAGAGLAAVYGLRMLGLFFILPVFAVHAVTLQGGHELT
                     LVGIAIGGYGLTQALLQIPFGAASDRWGRKRLIYAGLLVFAAGSFLGVVATDIWTAIA
                     ARILQGAGAINSVAMALAADLTREQHRTKIMAMIGATIGISFAVSLVG"
BASE COUNT          401 a          898 c          895 g          446 t
ORIGIN      
        1 gcgcgaacag cccggtgtac gttgccggat tggagcgcgg cgtgcgcccg atggggctct
       61 ggtcgaccgc gattactttg tcgatgtgct ccatgccggc gagcgcgccg tgcggcgccg
      121 gctccgccga cgcgccgtgc agggcgcgcg caagcgccgc gtacaaggtg tcgttcacca
      181 acgtggattt ccccgagccc gacacgccgg tgacgcagac cagaacgccg agcggaagct
      241 cgacatcgat gtccttcagg ttgttgcctc gcgcaccttg caacaccagc gtttgtttgg
      301 cgcgccggcg gtggggcggg accgcaatcg cggcctcgcg cgcgaggtag cggccggtga
      361 gcgactgtgg attggagatg acttccgcgg gagatccctg cgccacgatc cggccgccgg
      421 cctcgccggc tccgggcccc atgtcgatca cgtagtccgc ggcacggatc gcctcctcgt
      481 catgctccac cacgatcacc gagttgccca ggtcgcgcaa ctgcttgagc gtcgcgatga
      541 ggcgcgcgtt gtctcgctga tgcagcccga tcgagggctc gtccagcacg tacatcactc
      601 cggtcagccc cgagccgatc tggctcgcga gccgaatgcg ctgcgcttcg ccgccggaaa
      661 gcgtctcggc ggagcgttcg agcgagaggt attcgaggcc gacgttcatc aagaactcca
      721 ggcggctcgc gatctcgcgc acgatgcgct cggcgacctg cgccttggcg cccttgaggg
      781 tcaacgtgcg gaagaactcg ctcgcctgct tgagcggcca ggcggagatc tcctgaagcg
      841 tcgtgtcggc gacgcgcacg tggcgcgcct cgcgccgcag gcgcgcgccg ccgcattcag
      901 gacaggcgcg caagttgcgc agcttggcaa gctcctcgcg caccacctgc gagtcggtct
      961 cccggtagcg gcgctcgtgg ttcgggatca cgccctcgaa ggcgtgctcc ttcaccgcgg
     1021 cgcggccgcg ctccgacgga taccggaagg cgatggtttc gcttccggag ccgtacagga
     1081 tcagtttttg aatgcgctcg tcgagcatct cccacgggct ctcgatgtcg aagccgtagt
     1141 gccgggcgag cgattgcagc atggagaagt agaacgcgtt cctgcggtcc cagccgcgga
     1201 tcgcgccgcc cgcgagcgac aggttcgggt gggcgatgat gcgcttcggg tcgaaaaatt
     1261 ccaccgtgcc gagcccgtcg cagcgcgggc acgcacccgc ggggttgttg aaagagaaaa
     1321 gccgcggctc gagctccggc accgcgtagt cgcacaccgg gcaggagtaa cgcgccgaga
     1381 agaggtgctc cgcgccggtc tcgagctcga tcaccaccac cttgccgtcc gcatgccgca
     1441 aggcggtctc gagcgactcg gcgatccgct ggcgcgcgtc ggcgcgcacg cgcaggcggt
     1501 cgaccaccac gtcgaccgag tgcttgacgt tcttcgcgag ccggggcgtc gcgtcgagct
     1561 cgtacaccgc gccgtcgatg cgcacacgcg tgaagccctg cgcgcgcagc tcgccgagga
     1621 gatcggcctg ctcgcctttg cgcccggcga ccaccggcgc gaggatcatg acgcgggtcg
     1681 cctcgggcag cgcggcgacc tggtccacca tctcgctcac cgtctgggct tcgagcgcaa
     1741 gcccatgctg cgggcagtac ggcgtgccgg cgcgggcgaa aagaagcctg aggtagtcgt
     1801 gcacctcggt caccgtgccg acggtcgagc gggggttgtg gcttgcggcc ttctgttcga
     1861 tcgcgatggc gggcgagagc ccttcgatca ggtccacctc gggcttcgcc atcagctcga
     1921 gaaactggcg cgcgtacgcc gatagcgact ccacgtagcg gcgctggcct tcggcgtaca
     1981 gcgtatcgaa cgcaaacgag ctcttgcccg agcccgaaag cccggtgatc accaccagcc
     2041 ggttgcgcgg cagctcgacg ctcaggttct tgagattgtg ggtgcgcgct ccgcggacgc
     2101 ggatgagatc catgctgcgg cctgagaagt gaaacttgct actatagccc gctccacttt
     2161 cccgccgcca cacttctcat gactgaaagg atgagccgcg ccgagctgcg cgccggcgcc
     2221 ggcctggcgg ccgtgtatgg cctgcgcatg ctggggctct tcttcatcct cccggtcttc
     2281 gcggtgcacg ccgtgacgct gcaaggcggc cacgagctca cgctcgtcgg gatcgccata
     2341 ggaggctacg ggctaacgca agctcttctg cagattccgt tcggcgcggc gtccgaccga
     2401 tggggtcgaa agcggctgat ctacgccggc ctgctcgtgt tcgccgccgg aagctttctc
     2461 ggcgttgtcg cgacggatat ctggactgcg attgccgcgc gcatcctgca gggtgccggc
     2521 gcgatcaatt ccgtcgccat ggcgctcgcc gctgatctta cacgcgagca gcaccgcacc
     2581 aagatcatgg cgatgatcgg cgcgaccatc ggcatcagct tcgcggtctc gctcgtcgga
//
LOCUS       DASUNT010000083         3202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_975211, whole genome shotgun sequence.
ACCESSION   DASUNT010000083 DASUNT010000000
VERSION     DASUNT010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975211"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VFX94_01730"
     CDS             complement(<1..508)
                     /locus_tag="VFX94_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5766927.1"
                     /translation="MLQNREGQKVPQATFRTRTESGDWKTITSDELFKGKTVVVFSLP
                     GAFTPTCSSTHVPRYNELAPAFFANGVDRIICISVNDAFVMNEWARTQECGNVLMVPD
                     GNGEFTEKMGKLVDKTELGFGKRSWRYAMLVKDEVIEKMFIEPEKPGDPFEVSDADTM
                     LEYINPKAK"
     gene            complement(542..1315)
                     /locus_tag="VFX94_01735"
     CDS             complement(542..1315)
                     /locus_tag="VFX94_01735"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HEX5766928.1"
                     /translation="MKVLAALGYGLVNAFHPRMLWLMVWPMLVSLAVWLSVAFVFWAR
                     LAVWIAEVFKRWADPVAGWVPFDLGTAATFIANFMLLVLFFPLVYLTALFILGVFGMD
                     KMVNHVAAQISPSPERRRGGGIPGSVWNGLVSVGGMVLLFLASLPLWLIPPLGPIAVL
                     AILSWVNQRLLRYDALAQHAEKAEMSRIFVERRGGLYSLGLVLALAGYVPLLNLLAPV
                     VFGLAFIRYLLGALQDLRAISVPYDAHPNKELVRGNTPP"
     gene            complement(1312..>3202)
                     /locus_tag="VFX94_01740"
     CDS             complement(1312..>3202)
                     /locus_tag="VFX94_01740"
                     /inference="COORDINATES: protein motif:HMM:NF023326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEX5766929.1"
                     /translation="SWTKKPGKWIVAAELVETTRLFARTVANVEPRWLEELGGHLLKR
                     SRSDPHWEKGQGQVVALERGTLYGLPLYLNRRVPFAPHDPDLARDIFIRSALVEGDFD
                     SRAPFFAHNRRLVADIQRLEHKSRRPDILVDDELIHAFYDERIPKEINSEHSFERWRR
                     QAEAARPRLLHLAREDLMRHEAAGITTQNFPPALELGPNRYALEYHFEPRSPRDGVTL
                     NVPLALLNQVPAARTEWLVPGLLKQKVKALAKSIPQRLRHKLGSLEDFAAAFSAAVPP
                     ADLPLTEAMRRYIRTEFNLEVPLDAFRPDSAPPHLHMNFRLLDEHGRQLDMGRDLAAL
                     KRAHATRTQDIIHGEAPVEKTERYTGWTMGDLPDLMEIERGGQTLIGYPALVDERDGV
                     TLQVFDSPEKGRELHRAGVLRLLAIAFRDRIRDVERSLAKDVVLGPFKAEVVAAALTR
                     TFLQESVPTTQADFARRLDEGRSRLTLIAQEIARTASGILTERAFIEKKLNALAKTFP
                     AQTEDVKQQLGRLLAPGWLARTPWERLQHLPRYLKAASMRLDKLRADPARDARLAAEF
                     ASLEQPYRREAAARARHGAPGAEFEQFGWLLEELRVSLFAQELKTPVPVSVKRLGKLW
                     QTVRR"
BASE COUNT          556 a         1031 c         1084 g          531 t
ORIGIN      
        1 tcttcgcctt ggggttgata tattcgagca tcgtgtcggc atcggaaacc tcgaacgggt
       61 cgccgggctt ttcgggctcg atgaacatct tctcgattac ttcgtccttc accagcatcg
      121 catagcgcca cgagcgcttg ccgaagccga gctcggtctt gtcgaccagc ttgcccatct
      181 tctcggtgaa ttcgccgttg ccgtccggaa ccatgaggac gttgccgcat tcctgcgtgc
      241 gggcccactc gttcatgacg aaggcatcgt tcaccgagat gcagatgatg cgatcgacgc
      301 cattggcgaa gaacgcgggc gcaagctcgt tgtagcgcgg cacgtgggtg gacgagcacg
      361 tgggagtaaa ggcgccgggc agcgagaaga cgaccaccgt cttgcccttg aagagctcgt
      421 cgctagtgat cgtcttccag tcgccgcttt cggtgcgggt gcggaaagtg gcctggggaa
      481 ccttctggcc ttcgcggttt tgcagcactg tggatcctct cgaaaaagaa tggactgcag
      541 tctacggcgg cgtgttcccg cggaccaatt ctttgttcgg atgggcgtca tagggaacgc
      601 taattgcacg caggtcctgg agcgcgccca gcaggtaacg gatgaaggca agcccaaaca
      661 ccaccggcgc gaggaggttg agaagaggga cataacccgc cagggcgagc accaatccga
      721 ggctataaag cccgccacgg cgctcgacga agattcggct catttccgcc ttttccgcgt
      781 gctgggcgag ggcgtcgtag cgcagaagcc gctggttgac ccaggacagg atggccagca
      841 cggcaatcgg accgagcggc gggataagcc acaacggcag gcttgccagg aacagcagca
      901 ccatgccgcc caccgatacc aggccgttcc agacgctgcc cgggatgccg ccgcccctgc
      961 gccgctccgg tgacggagaa atctgcgcgg caacgtgatt gaccatcttg tccatgccga
     1021 acacgccgag gatgaagagc gcagtcaggt agaccagcgg aaagaagagc accagcagca
     1081 tgaaatttgc aatgaacgtg gcagccgtgc cgagatcgaa aggcacccag ccggcgaccg
     1141 ggtcggccca ccgcttgaac acttccgcga tccagactgc cagtcgcgcc cagaaaacga
     1201 acgccacgga cagccacacc gcgagcgaca cgagcatcgg ccagaccatc agccatagca
     1261 tgcgcgggtg gaatgcgttc accagaccgt agccgagcgc ggccaggact ttcatcggcg
     1321 gacagtctgc cagagtttcc ccaggcgctt gaccgagacg gggaccgggg tttttagctc
     1381 ctgcgcgaaa agcgagacgc gcagctcttc cagcagccag ccgaactgct cgaattccgc
     1441 tcccggcgcg ccgtggcgcg cgcgcgccgc cgcttcgcgc cggtacggct gctcgagcga
     1501 ggcgaactcg gcggcgaggc gcgcgtcgcg cgccggatcg gcgcgcagct tgtcgagccg
     1561 catggaagcc gccttgaggt agcgcggcag gtgctgcagc cgctcccacg gtgtccgcgc
     1621 cagccatccc ggcgcgagca ggcggccgag ctgctgcttg acgtcttcgg tttgtgccgg
     1681 aaaggtcttc gccagcgcat tcagcttctt ttctatgaag gcgcgctcgg tcaggatgcc
     1741 ggacgcagtg cgcgcgatct cctgcgcaat gagggtcaag cggcttcgcc cctcgtcgag
     1801 ccgccgcgca aagtcagcct gggtggttgg tacggattcc tggaggaacg tgcgagtcag
     1861 cgccgccgcg accacctccg ccttgaaagg gcccaagacc acatccttgg cgagcgagcg
     1921 ctccacgtca cgaatacgat cgcggaacgc aatcgcgagg agccgcagca cgccggcgcg
     1981 gtgcagctcg cggccctttt ctggcgaatc gaagacctgc agggtaacgc cgtcgcgttc
     2041 gtccacgagc gcggggtagc caataagcgt ctggccgccg cgctcgatct ccatcagatc
     2101 gggcaggtcg cccatcgtcc agccggtgta gcgctcggtc ttctcgaccg gcgcttcgcc
     2161 gtggatgatg tcctgcgtcc tggtcgcgtg cgcgcgcttg agcgcggcca ggtcgcgccc
     2221 catgtcgagc tggcgcccat gctcgtcgag gaggcggaag ttcatgtgca ggtgcggcgg
     2281 cgccgagtcc ggccggaacg cgtctagcgg gacctcaagg ttgaactcgg ttcgtatgta
     2341 gcggcgcatc gcctcggtga gaggcagatc ggcaggcggc acggcggcgc tgaacgcggc
     2401 ggcgaaatcc tccagcgagc ccagcttgtg gcgcaggcgc tgcggaatcg acttggcgag
     2461 cgccttcacc ttttgcttca acagtcccgg caccagccac tcggtgcgcg ccgccggcac
     2521 ctggttgaga agcgcgagcg gcacgttgag cgtcacgccg tcgcgcggcg agcgcggctc
     2581 gaagtggtac tcgagcgcgt agcggttggg gccgagctcg agcgccggcg ggaagttctg
     2641 cgtcgtgatg cccgccgcct cgtggcgcat gaggtcctcg cgcgcgaggt gcagcaggcg
     2701 cggcctcgca gcctccgctt ggcgccgcca acgttcaaat gaatgttcgg aattaatttc
     2761 cttggggatg cgctcgtcgt agaaagcgtg gatcagctcg tcgtcgacca ggatgtccgg
     2821 gcggcgcgac ttgtgctcga ggcgctggat gtccgccacc aggcgccggt tgtgcgcgaa
     2881 gaacggcgcg cggctgtcga agtcgccctc gaccagcgcc gagcggatga agatgtcgcg
     2941 cgcgaggtcg gggtcgtgcg gcgcgaaggg cacgcggcgg ttgaggtaga gcggcagccc
     3001 gtacagcgtg ccgcgctcga gcgccacgac ctggccctgg cccttctccc agtgcgggtc
     3061 gctgcggctg cgcttgagca ggtggccgcc gagctcctcc agccagcgcg gctcgacgtt
     3121 cgccacggtg cgcgcgaaca ggcgcgtggt ctccaccagc tccgcggcga cgatccactt
     3181 gcccggcttc ttcgtccacg ag
//
LOCUS       DASUNT010000084         3887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_800278, whole genome shotgun sequence.
ACCESSION   DASUNT010000084 DASUNT010000000
VERSION     DASUNT010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800278"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..896)
                     /gene="serB"
                     /locus_tag="VFX94_01745"
     CDS             complement(69..896)
                     /gene="serB"
                     /locus_tag="VFX94_01745"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEX5766930.1"
                     /translation="MNLVVQGPALEEQQATEIARMAGAALAPIAERAYRLRNARRSRD
                     IASWCEARRIDCAWIPEGRRFADLKLLAMDMDSTLITIECIDELGDLAGRKAEIAAIT
                     AQAMRGEIEYRESLRRRVAALAGLPEDSLKSVYEGRLELTPGADALVGACKKHGVKVL
                     LVSGGFTFFTDRLKARLGIDYTISNTLEVKAGRLTGALVGDIVDAGAKAAKFKAVLRE
                     VNAGKEQSVAIGDGANDLRMMREAGISVAFRAKPVVRAQATCALNWSGLDGVVNLFE"
     gene            complement(893..>3887)
                     /gene="mfd"
                     /locus_tag="VFX94_01750"
     CDS             complement(893..>3887)
                     /gene="mfd"
                     /locus_tag="VFX94_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX5766931.1"
                     /translation="AVAGYQHVTQVVAPGEVCFRGGLIDLFPMGSALPYRLDLDDDVL
                     DAIKTFDVDTQRTLYSVSEIRMLPAREFPLDDAGRARFRSRWRELFEGDPSKKPLYRD
                     VSSGVPAAGIEYYLPLFFDSVATLFDYLPAASVLALHHDVSDAVQEFWRDASSRHKMA
                     GGDPDRPLLPPPQLFVPAEEFYVRAQAFPRIDIVDKPDQEGEALIASALPALAVDRRA
                     QDPLAALKRFVDTSGGRVLISAESPGRRETMASYFAEYGLRPEPCSGFADFIARRSKC
                     MLGVAPLARGFAVPAEGWAVVTEAELYAGVVRRRGRVAEKRGSVEGMLRDLSELKVGD
                     PVVHEQHGIGRYQGLVSLDVGDGNTEFLLLEYEGGDKLYVPVAQLGVIGRYSGAQPEE
                     APLHKLGSGQWDKAKARAAKQVRDTAAELLALYARRAARRGHSFSIRQHDLEAFADGF
                     GFEETPDQAAAINSVIADMAAGKPMDRLVCGDVGFGKTEVALRAAFAAVADGCQVVML
                     CPTTLLAEQHLQTFSDRFADWPVRIAELSRFRSGTESADIVRKLESGEIDIVIGTHKL
                     LSKGVKLKRLGLVIIDEEHRFGVRQKEALKKLRAEVDVLTLTATPIPRTLAMSLEGIR
                     DFSVIATAPQKRLAIKTFVAPWSEGLIREAVMRELRRGGQAYFLHNEVETIERMRERL
                     AKLLPEARIGAAHGQMRERELERVMRDFTAQRVNVLLCSTIIETGIDIPTANTIVINR
                     ADRFGLAQLHQLRGRVGRSHHQAYAYLLTPPEEALNKNALKRLEAIQLMEELGSGFYL
                     AMHDLEIRGAGEILGEAQSGEIQEVGFTLYARMLDRAVRRLKSGKTGDAEDAIDISTE
                     VNLHVPALLPAAYCSDVHERLSLYKRLADADTREALDLLREELVDRFGELPEPARALI
                     ECHRVRIAARPLGVARVDVTHEAVQLQFVKNPPLDGTKVIDFIRRKGRAARLAGPEKL
                     RVEAKLPAWQERAQAVKDILHQLAA"
BASE COUNT          590 a         1330 c         1320 g          647 t
ORIGIN      
        1 acgtcgtcaa cctcgggtcc ggcatcgaag gctggcgcgg ggccggcctt ccggttagcg
       61 ccggctagct actcgaacag gttcaccacg ccgtccagcc cgctccagtt gagcgcgcac
      121 gtcgcctgcg cgcgcaccac gggcttcgcg cggaaggcga cggagattcc ggcttcgcgc
      181 atcatcctga ggtcgttggc gccatcgccg atcgcgacgc tttgctcttt gccggcgttc
      241 acttcgcgca agacggcctt gaacttggcc gccttggcgc cggcgtcgac gatgtcgcct
      301 accagagcgc ccgtaagcct gccggccttc acctcgagcg tgttcgagat cgtgtagtcg
      361 atgcccaggc gcgcctttag cctgtcggtg aagaacgtga agccccccga caccagcagc
      421 accttcacgc cgtgcttctt gcatgcgccg acgagtgcgt ccgctccggg cgtaagctcg
      481 agacggcctt cgtaaacgct tttcaaagaa tcttctggca gtccggcgag cgccgcgacg
      541 cgccggcgca ggctttcgcg gtactcgatc tctccgcgca tcgcctgggc ggtgatcgct
      601 gcgatctcgg ccttcctgcc cgcaaggtcg ccgagctcgt cgatgcactc gatggtgatg
      661 agggtggaat ccatatccat ggcgaggagc ttgaggtcgg cgaaacgccg cccttcggga
      721 atccaggcgc agtcaatgcg ccgtgcttcg caccaggaag cgatgtccct gctccgccgg
      781 gcgtttcgca gccgatatgc gcgctcggcg atcggcgcga gcgctgcccc cgccatgcgg
      841 gcgatttccg ttgcttgctg ctcctctagg gcgggaccct ggacgaccag gttcatgccg
      901 cgagctggtg caggatgtcc ttcacggcct gcgcacgctc ctgccaggct gggagcttcg
      961 cctccacgcg gagcttctcc ggaccagcga gccgtgcggc ccggcctttg cggcggataa
     1021 agtcgataac cttggttccg tcgagcggcg ggttcttgac gaactgcagc tgcaccgcct
     1081 cgtgggtgac gtccacgcgc gcaacgccca gcggccgcgc cgcgatgcgc acgcggtggc
     1141 actcgatgag cgcgcgcgcg ggctcgggca gctcgccgaa gcggtcgacg agctcctcgc
     1201 gcagcagatc cagcgcctcg cgcgtgtcgg catccgcgag gcgcttgtac agcgagagcc
     1261 gctcgtgcac gtcgctacag taggccgccg gaagcagcgc gggcacgtgg aggttgacct
     1321 cggtggaaat gtcgatcgca tcctcggcgt ccccagtctt cccggatttc agccggcgca
     1381 ccgcccggtc gagcatgcgc gcgtagaggg taaagccgac ctcctggatc tcgcccgact
     1441 gcgcttcgcc caggatctcg cccgcgccgc ggatctcgag atcgtgcatc gcaaggtaga
     1501 agcccgagcc gagctcctcc atcagctgga ttgcctccag gcgcttcagc gcattcttgt
     1561 tcagcgcttc ttcgggcggc gtcagcaggt acgcatacgc ctgatggtgc gagcggccga
     1621 cgcgcccgcg cagctggtgc agctgggcaa gcccgaagcg gtcggcgcgg ttgatcacga
     1681 tggtgttcgc ggtcgggatg tcgatcccgg tctcgatgat ggtggagcac agcagcacgt
     1741 tgacgcgctg cgcggtgaag tcgcgcatca cgcgctctag ctcgcgctcg cgcatctggc
     1801 cgtgcgccgc cccgatgcgc gcctcgggca ggagcttcgc caggcgctcg cgcatgcgct
     1861 cgatggtctc cacctcgttg tgcagaaagt acgcctggcc gccgcgtctc agttcgcgca
     1921 tcaccgcctc gcggatcagc ccctcggacc acggcgcgac gaaggtcttg atcgcgaggc
     1981 gcttctgtgg cgcggtggcg atgacggaga agtcgcggat gccttcgagc gacatcgcga
     2041 gcgtgcgcgg aatcggcgtc gccgtgagcg tcagcacgtc gacctcggcg cgcagcttct
     2101 tcagcgcctc cttctgccgc acgccgaagc ggtgctcctc gtcgatgatc acgagcccga
     2161 ggcgcttgag cttgacgccc ttcgacagca gcttgtgcgt gccgatcacg atgtcgatct
     2221 cgccgctttc gagtttgcga acaatgtccg cggattctgt tccgcttcga aagcgggaca
     2281 attcggcaat gcgaaccggc cagtcggcga agcggtccga gaaggtctgc aaatgctgct
     2341 cggccagcag agtcgtgggg cagagcatta cgacctggca gccgtcggcg accgcggcga
     2401 aggcggcgcg caacgccacc tcggtcttgc cgaagccgac gtcgccgcac accagtcggt
     2461 ccatgggctt tcccgcggcc atgtcggcga tcaccgagtt gatcgccgcc gcctggtcgg
     2521 gcgtctcctc gaagccgaag ccgtcggcga aggcctccag gtcgtgctgc ctgatcgaga
     2581 acgagtgtcc gcgccgggcg gcgcgcctgg cgtagagcgc cagcagctcg gccgcggtgt
     2641 cgcgcacctg cttcgccgcg cgcgccttcg ccttgtccca ctggccgctg ccgagcttgt
     2701 gcagcggcgc ctcttcgggc tgcgcgccgc tgtagcggcc gatcacgccg agctgcgcga
     2761 ccggtacgta gagcttgtcg ccgccttcgt actcgagcag caggaactcc gtgttgccgt
     2821 cgccgacgtc gaggctgacg agtccctgat aacgtccgat gccatgctgc tcatgcacca
     2881 ccgggtcgcc gactttgagc tcggagagat cgcgcagcat gccttcgacc gagccgcgct
     2941 tctcggcgac gcgcccgcgc cggcgcacca cgccggcgta gagctccgcc tcggtgacga
     3001 ccgcccagcc ctcggcgggt acggcgaagc cgcgcgcgag cggcgccacg ccgagcatgc
     3061 acttcgagcg ccgggcgatg aagtcggcga agccggagca gggctcgggt cgcagcccgt
     3121 attccgcgaa gtagctcgcc atggtttcgc gccgtccggg cgactcggcc gagatcagca
     3181 cgcgcccgcc cgaggtgtcg acgaagcgct tcagcgctgc aagcggatcc tgcgcgcgcc
     3241 ggtcgaccgc aagcgcagga agcgcgctgg cgatgagcgc ttcgccttcc tgatccggct
     3301 tatcgacgat gtcgatgcgc ggaaaggcct gcgcgcgcac gtaaaactct tcggccggga
     3361 cgaagagctg gggaggtgga agaaggggcc ggtcgggatc gccgcccgcc atcttgtggc
     3421 gcgagctggc atcgcgccag aactcctgga ccgcatcgga aacgtcgtgg tgcaaggcaa
     3481 ggacgctcgc cgccggcagg tagtcgaaaa gcgtcgcgac cgaatcgaag aagagcggca
     3541 ggtaatactc gattcccgcg gccggcacgc cgctcgagac gtcgcggtag agcggcttct
     3601 tcgacgggtc gccctcgaag agctcgcgcc agcggctgcg aaagcgcgcg cggcccgcgt
     3661 cgtcgagcgg aaactcgcgc gccggcagca tgcggatctc gctcacggag tacagcgtgc
     3721 gctgggtgtc gacgtcgaag gtcttgatgg cgtcgagcac gtcgtcgtcg aggtccagcc
     3781 ggtagggcag cgcggatccc atcggaaaga gatcgatcag gccgccccgg aagcagacct
     3841 cgccgggcgc gaccacctgg gtcacgtgct ggtacccggc gacggcg
//
LOCUS       DASUNT010000085         2051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_449767, whole genome shotgun sequence.
ACCESSION   DASUNT010000085 DASUNT010000000
VERSION     DASUNT010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449767"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VFX94_01755"
     CDS             complement(<1..765)
                     /locus_tag="VFX94_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5766932.1"
                     /translation="MKRFLIIPVLLLVSAASAQTLRIGLAEDPDILDPTMARTFVGRI
                     VFAGLCDKLFDLDEKLNIVPQLATSHKWSADNKSLEIKLRDGVTFHDGEKLDAAAVKY
                     NLERHKNLKGSSRRGELAVVSSVDAVDASTVRINLAAPFAPLLAVLTDRAGMMVSPKA
                     AEAAGEKFGANPVCSGPFRFVERIAQDRIVLERFANYWNKGEIHFQRIVYQPIVDATV
                     RLANLRSGQLDFIERVAPSDIPQLRNDNRFRIAKIVE"
     gene            complement(762..1772)
                     /locus_tag="VFX94_01760"
     CDS             complement(762..1772)
                     /locus_tag="VFX94_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX5766933.1"
                     /translation="MSQPLVRARRLVKHFKKVQAVDDVNFDVYPGETLALVGESGCGK
                     STTGKLLLRLIEPTSGVVYFRQQNIFELRERDMRSLRSEMQIIFQDPYGSLNPRMTVG
                     DMLEEALRLHKLAVGQERARVIELLGLVGLAPDHASRYPHEFSGGQRQRIGIARALAV
                     EPKLIVCDEPVSALDVSIQAQVINLLRELQRRFGLAYVFIAHDLAVVKHIATRVAVMY
                     LGKIVEIGEKRALFAAPRHPYTRALLSAIPVPDPLLKRPRIILEGDVPSPYDPPSGCR
                     FRTRCPWARERCAAEEPVLGSGVACHFWKELEPLPAASQLTAVNERLLRLQAAFQSGG
                     SP"
     gene            complement(1769..2038)
                     /locus_tag="VFX94_01765"
     CDS             complement(1769..2038)
                     /locus_tag="VFX94_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX5766934.1"
                     /translation="MVERCAATRLFAEPQHPYTVGLLGSIPRLHLEQERLSAIEGIVP
                     DAAAFPQGCRFHPRCPFATDQCRSEVPVLTEVRPKHYAACWRAPL"
BASE COUNT          370 a          643 c          703 g          335 t
ORIGIN      
        1 ctccacgatc ttggcgatgc ggaagcggtt gtcgttgcga agctgcggga tgtcggaagg
       61 cgcgacgcgc tcgatgaagt cgagctggcc cgagcgaagg ttggcaagtc gcacggtcgc
      121 gtccacgatc ggctggtaca cgatgcgctg gaagtggatc tcgcccttgt tccagtagtt
      181 cgcgaagcgc tcgaggacga tgcggtcctg ggcgatgcgc tcgacgaagc ggaacgggcc
      241 cgagcagacg ggattcgccc cgaatttctc gcccgccgcc tcggcggcct tcggcgagac
      301 catcatgccg gcgcggtcgg tgaggacggc gagcagcggc gcgaagggcg cggccaggtt
      361 gatccgcacc gtggaggcgt ccaccgcatc gacgctcgac acgacagcga gctcgccgcg
      421 gcggctcgag cccttcaggt tcttgtggcg ctcgaggttg tacttgaccg cggcggcgtc
      481 gagcttctcg ccgtcgtgga acgtgacccc gtcgcgcagc ttgatctcga gcgacttgtt
      541 gtcggccgac cacttgtggg acgttgcgag ctgcggcacg atattcagct tctcgtcgag
      601 gtcgaaaagc ttgtcgcaca ggcccgcgaa cacgatgcgg ccgacgaagg tgcgcgccat
      661 cgtcggatcg aggatgtcgg gatcttctgc gaggccaatt ctcagcgtct gagcactcgc
      721 agccgagacg agaagcaaga ccgggataat cagaaagcgc ttcatggtga acctcctgat
      781 tggaacgctg cctgaaggcg taacagtctt tcattgaccg cagtcaattg cgaggccgcg
      841 ggcaagggct cgagctcttt ccagaaatgg caggcgacgc cggagccgag caccggctct
      901 tccgcggcgc agcgctcgcg cgcccacgga caacgggtgc ggaagcggca gcccgagggc
      961 ggatcgtagg ggctcggaac gtcgccttca agaatgatgc gcggtctttt caataatgga
     1021 tcgggaacgg ggatcgccga gaggagcgcc cgcgtatagg gatggcgcgg cgcggcgaaa
     1081 agcgcccgct tctcgccgat ttcgacgatc ttgccgaggt acatgaccgc gacgcgcgtg
     1141 gcgatgtgct tcaccaccgc cagatcgtgc gcgatgaaga cataggcgag gccgaaccgg
     1201 cgctgcagct cccgcagcag attgatgact tgcgcctgga tcgaaacgtc gagcgccgac
     1261 accggctcgt cgcacacgat gagcttcggc tcgaccgcga gcgcgcgcgc gatcccgatg
     1321 cgctggcgct gcccgccgga gaactcatgc ggatagcgcg aagcatggtc gggcgcgagc
     1381 ccgaccagcc caagcagctc aatcaccctg gcgcgctctt gaccgacggc gagtttgtgc
     1441 aggcgtaaag cctcctcgag catgtcgccg acggtcatgc gcggattcag cgagccgtac
     1501 gggtcctgga agatgatctg catctcgctg cgcagcgagc gcatgtcccg ctcgcgcagc
     1561 tcgaagatgt tctgctgcct gaagtaaacg acgcccgagg tgggctcgat caggcgcagc
     1621 agcagcttgc cggtggtcga cttgccgcag cccgattcgc cgacgagcgc gagcgtctcg
     1681 ccgggataca catcgaagtt cacgtcgtcc accgcctgga ccttcttgaa gtgcttgacc
     1741 agccggcgcg cgcggacgag cggctggctc ataggggcgc gcgccagcac gcggcgtaat
     1801 gcttcggcct tacttctgtc aacaccggaa cctccgatcg gcattgatct gtcgcgaacg
     1861 ggcagcgcgg atgaaaccgg catccctgcg ggaacgcggc ggcatcggga acgatcccct
     1921 cgatcgcgct caggcgctcc tgctcgagat gcaaccgcgg gatcgagccg agcaggccga
     1981 cggtgtacgg atgctgcggc tcggcaaaga ggcgggtcgc agcgcagcgc tcgaccacct
     2041 ggccggcata c
//
LOCUS       DASUNT010000086         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1063712, whole genome shotgun sequence.
ACCESSION   DASUNT010000086 DASUNT010000000
VERSION     DASUNT010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063712"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1483)
                     /locus_tag="VFX94_01770"
     CDS             complement(<1..1483)
                     /locus_tag="VFX94_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX5766935.1"
                     /translation="MRQFRQILLWPLQLMPIREGEQIQEHWEVLQRGGAESAWRELTD
                     EFTGDPAQFQERHYSEFVTFLPYVQRFLYGEGKERGTPASESPIRVFRRGDVARVRMT
                     FPGDEANPVVFDVAHVDLYFFYDIDVAILVVEIHARDLSLARAQDTLYRFGRTYPTYW
                     NDDGSGGHCVRRVEWLSAAGEVLAVSDYERRDKFLGAVCSYRAPAFGGHWEFLLRPLV
                     PHHSDEKGPIRFRVVEYHRMPLLGYLALEDPRRMTRGDFMRLAFVTAAGESAALPFSE
                     RHAHDFEWRYCYDRYWNEHAEGPSGTRYMCTGHSFMMVGSAADPFFTDAERGLLGQFR
                     HQYFLLFLIPHFHKASLLMFSDRLVDALNRMDIHDAESVRRFKRAIRYIKEIFLRFTH
                     RYWFHEVSDQAQARELFRMSREYLDTERLYAQVREEIQDMSEYLDSDSQRRQSNTIVR
                     LTVVTTFGLIGTVAASFLGMNLIAEADNPLALKFAYFLRVFVPV"
     gene            complement(1561..>2005)
                     /locus_tag="VFX94_01775"
     CDS             complement(1561..>2005)
                     /locus_tag="VFX94_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5766936.1"
                     /translation="MSGIDLYAELKRRGSRMPAIIMTGHGDVSVAVRAMKAGVYDFIE
                     KPFKDQALLDLIQQALAQCSQAAGQAAQRGEIAACARTLTRRERQVMDLVVTGKLNKL
                     IAAELGLSTRTVELHRARVMEKMQAKTFSDLVRMAIALQAEERSP"
BASE COUNT          331 a          667 c          683 g          324 t
ORIGIN      
        1 ttacgggtac gaacacacgc aagaagtagg cgaacttgag cgccagcggg ttgtcggcct
       61 cggcgatcag gttcatgcca aggaagctcg cggccaccgt gccgatcagg ccgaaggtcg
      121 tgaccaccgt cagccggacg atcgtgttgg attgccggcg ctgcgagtcg ctatcgagat
      181 actcgctcat gtcctggatc tcctcgcgca cctgcgcgta caggcgctcg gtgtcgaggt
      241 actcccggct catgcggaac agttcgcgcg cctgcgcctg gtcggaaacc tcgtggaacc
      301 agtagcggtg ggtgaagcgc aggaagattt ccttgatgta gcggatcgcc cgcttgaagc
      361 gccttacgct ctccgcgtcg tggatatcca tgcggttgag cgcgtccacc aggcggtccg
      421 agaacatcag cagcgacgcc ttgtggaaat gagggatcag aaacagcagg aaatactggt
      481 gccggaactg cccgagcagt cccctctccg cgtcggtgaa gaagggatcg gctgcgctcc
      541 ccaccatcat gaacgaatgg ccggtgcaca tgtagcgcgt gccggacggg ccctcggcgt
      601 gctcgttcca gtagcggtcg tagcagtagc gccactcgaa atcgtgcgcg tgccgctccg
      661 agaagggcag cgcggccgac tcgcccgcgg cggtgacgaa cgcgaggcgc atgaaatcgc
      721 cgcgcgtcat gcggcgcggg tcctcgagcg ccaggtagcc gagcagcggc atgcggtggt
      781 actcgacgac gcggaagcgg atcggcccct tctcgtccga gtggtgcggc accagcggcc
      841 gcagcaggaa ctcccagtgc ccgccgaagg cgggcgcgcg atagctgcac accgcgccga
      901 gaaacttgtc gcgccgctcg taatcggaga cggcgagcac ctcgcccgcg gccgagagcc
      961 actcgacgcg ccgcacgcaa tggccgcccg agccgtcgtc gttccagtag gtgggatagg
     1021 tgcggccgaa gcggtacagg gtgtcttgcg cgcgcgccag gctcaggtcg cgggcatgga
     1081 tctcgaccac gaggatggcg acgtcgatgt cgtagaagaa gtacaggtcg acgtgcgcga
     1141 cgtcgaacac caccgggttc gcctcgtcgc cggggaacgt catgcgcacc cgggcgacgt
     1201 cgccgcggcg gaagacgcgg atcggcgatt cgctcgccgg cgtgccgcgc tccttgccct
     1261 cgccgtagag gaagcgctgc acgtacggca agaaggtcac gaactcgctg tagtggcgct
     1321 cctggaactg cgccggatca ccggtgaact cgtcggtgag ctcgcgccag gcactctccg
     1381 cgccgccgcg ctgcagcacc tcccagtgct cctggatctg ctcgccctcg cggatcggca
     1441 tcagctgcag cggccagagc aggatctggc ggaactggcg cacgatcgtc gccctcgccg
     1501 gccccaatcg gctggcgcag aagcgccccg tccagttccc ggtccatggg cgtcagtgtg
     1561 ttaaggcgaa cgctcctcgg cctgtagggc gatcgccatg cgcacgaggt cggagaaggt
     1621 cttcgcctgc atcttctcca tcacgcgcgc gcggtgcagc tccacggtgc gcgtgctgag
     1681 cccgagctcc gcggcgatca gcttgttcag cttcccggtg accacgaggt ccatgacctg
     1741 acgctcgcgc cgcgtcaggg tgcgcgcgca ggccgcgatt tcgccgcgct gcgccgcctg
     1801 gccggcggcc tgcgagcact gcgcgagcgc ctgctggatg aggtcgagca gcgcctggtc
     1861 cttgaacggc ttctcgatga agtcgtatac cccggccttc atggcgcgca ccgcaacgct
     1921 cacgtcgccg tgcccggtca tgatgatggc cggcatccgg ctgccgcggc gcttgagctc
     1981 ggcgtacaga tcgattccgc tcatg
//
LOCUS       DASUNT010000087         2821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_712898, whole genome shotgun sequence.
ACCESSION   DASUNT010000087 DASUNT010000000
VERSION     DASUNT010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712898"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..175
                     /locus_tag="VFX94_01780"
     CDS             8..175
                     /locus_tag="VFX94_01780"
                     /inference="COORDINATES: protein motif:HMM:NF023113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2380 domain-containing protein"
                     /protein_id="HEX5766937.1"
                     /translation="MSNLILNLNMEVRSVASGETLYAKSVDIRGNTDESWLRGVRRLV
                     DNIEERQHHLR"
     gene            complement(234..620)
                     /locus_tag="VFX94_01785"
     CDS             complement(234..620)
                     /locus_tag="VFX94_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766938.1"
                     /translation="MFLLCAAASACAAAIEPGRYAIVARTEMPHLEDALRYATTREER
                     CLRGDEPGSFFPILHHPALQGCALAGERPHYRLVCASPEVATGSARLDESARRIAGVL
                     EVKMGGKNMTFAQRIEAVRQGRCEMP"
     gene            724..1299
                     /gene="gfa"
                     /locus_tag="VFX94_01790"
     CDS             724..1299
                     /gene="gfa"
                     /locus_tag="VFX94_01790"
                     /EC_number="4.4.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051907 - S-(hydroxymethyl)glutathione
                     synthase activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione synthase"
                     /protein_id="HEX5766939.1"
                     /translation="MSNIKIHPQVDGGLKPAAADFAGGTLWCNCSKDRVQVAISAQVA
                     HNHVCGCTKCWKPKGALFSQVAVVPRDKVRVEANGHKLAIVDPKATIQRYACKECGVH
                     MYGRIENKGHPFYGLDFVHTELSKEQGWQPAQFAAFVSSIIESGGDPNNLSAGRSRLK
                     ELKLEPYDCLSPPLMDAIATHAATASGALRS"
     gene            complement(1320..2159)
                     /gene="fghA"
                     /locus_tag="VFX94_01795"
     CDS             complement(1320..2159)
                     /gene="fghA"
                     /locus_tag="VFX94_01795"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEX5766940.1"
                     /translation="MTLAKVSGSRCFGGTQSVYSHEARETGCPMRFGVFLPPQAEAQR
                     VPVLYWLSGLTCTEENFIVKAGAQRLAAELGLALVVPDTSPRGLNIPGESESYDFGLG
                     AGFYVDATQAPWSRGYRMYSYVTKELPQLIESSFPIERARTGVFGHSMGGHGALSVAL
                     KNQDCYKSVSAFAPISSPMRCPWGEKALTGYLGTERASWREYDATALIEERGWRGPAL
                     LVDQGSADPFLETQLKPGLLEEACRRAGVPLTLRLQAGYDHSYFFIATFIEEHLRFHA
                     RNL"
     gene            complement(2176..>2821)
                     /locus_tag="VFX94_01800"
     CDS             complement(2176..>2821)
                     /locus_tag="VFX94_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5766941.1"
                     /translation="REDAPFDKVCYIGCGVTTGIGAVINTAKVEPGANVVVFGLGGIG
                     LNVVQGARMVGANKIIGVDLNPARKALGEKFGMTHFVNPKEVQGDLVAHLVELTGGGA
                     DYSFECIGNVDVMRQALECCHRGWGVSVIIGVAGAGQEIKTRPFQLVTGRVWKGTAFG
                     GAKGRRDVPKIVDWYMEGKINIDDLITHKLKLADINEGFDLMHAGRSIRAVVVY"
BASE COUNT          497 a          927 c          917 g          480 t
ORIGIN      
        1 gcagaaggtg agcaacctga tcctcaacct caatatggag gtgcgcagcg tggcgagcgg
       61 ggaaacgctg tacgccaagt cggtcgatat ccgcggcaat accgacgagt cgtggctgcg
      121 cggcgtgcgc cgtctcgtcg acaacatcga agagcgccag caccacctgc gctaggcctg
      181 tcccaaaacg gaacacaggc atccaccgat ccggacacag agcctgtcga acgctaaggc
      241 atttcgcagc ggccctgccg cacggcctcg atgcgctgcg cgaaagtcat gttcttgccc
      301 cccatcttta cttcaaggac gcccgcgatg cggcgcgcgc tctcatcgag acgtgcgctt
      361 cccgtcgcga cctccgggct agcgcaaacg agtctgtaat gtggacgctc accggccaga
      421 gcgcagcctt gcagcgcggg atggtgcagg attgggaaga aggagccggg ctcgtcgccg
      481 cgcaggcagc gctcctcgcg cgtcgtggcg taacgcaagg cgtcctcaag atgaggcatt
      541 tcggtgcggg cgacgatcgc gtagcggccc ggctcgattg ccgcggcgca ggccgaggct
      601 gcagcgcaga gcaggaacac gaggacgttg ccattgcggg gccctcgggt gcaagatgat
      661 cgccgcgcgt cttctacgcg cttcacgcgc ttttgttcgg taggcaagag aggagagatc
      721 ggcatgagca atatcaagat acatccacag gtcgatggtg gactgaagcc tgcggcggcg
      781 gattttgccg gcggaacgct gtggtgcaac tgttcgaagg acagagtaca ggtcgcgatc
      841 agcgcgcagg tcgcgcacaa ccacgtctgc ggctgcacga agtgctggaa gccgaaaggg
      901 gcgctgttct cccaggtggc cgtggtcccg cgcgacaagg tcagagtgga agcgaacggc
      961 cacaagctcg ccatcgtcga ccccaaggcg acgatccagc gctatgcctg caaggagtgc
     1021 ggcgtgcaca tgtacggccg catcgagaac aagggccatc ccttctacgg tctcgatttc
     1081 gtccacaccg agctttcgaa ggagcagggc tggcagccgg cgcaattcgc cgccttcgtg
     1141 tcgtcgatca tcgagtcggg cggcgatccc aacaacctgt ccgcggggcg gagccggctg
     1201 aaggagctca agctcgagcc ttacgactgc ctgtcgccgc cgctgatgga tgcgatcgcg
     1261 acgcatgccg ccacagcctc gggagccctg cgctcgtaac gcgcgcggcg cccgttcgct
     1321 cacaggttgc gggcgtggaa ccgcagatgc tcttcgatga aggtggcgat gaagaagtag
     1381 ctgtggtcgt acccggcttg caggcgaagg gtcagcggca cacccgcgcg cctgcaagct
     1441 tcctccagca gcccgggttt tagctgcgtc tcgagaaacg ggtcggcgct gccttgatcg
     1501 acgagcagcg ccggcccgcg ccagccgcgc tcctcgatga gcgcggtggc gtcgtattcc
     1561 cgccaggagg cgcgctcggt cccgaggtaa ccggtgagcg ctttctctcc ccagggacag
     1621 cgcatcgggg aggagatcgg cgcgaacgcc gacaccgact tgtagcaatc ctgattcttg
     1681 agcgcgacgc tgagcgcgcc gtgcccgccc atcgaatgtc caaagacccc ggttcttgct
     1741 cgttctatgg gaaaggacga ttcgatcagc tgcggtaatt ctttcgttac gtacgagtac
     1801 atccggtagc cgcgcgacca gggcgcctgg gtggcgtcga cgtagaaccc cgcgcccagg
     1861 ccgaagtcgt agctctcgct ctcccccggg atgttcaggc cgcgcgggct ggtgtcgggt
     1921 acgacgagcg ccagaccgag ctcggcggcg aggcgctgcg cgcccgcctt gacgatgaaa
     1981 ttctcttcag tgcaggtgag ccccgaaagc cagtagagca ccggcacccg ctgcgcttcg
     2041 gcttgcggcg gaaggaacac gccgaagcgc atcgggcagc cggtctcgcg cgcctcgtgg
     2101 ctgtagacgc tttgggttcc gccaaagcat ctactcccgg agactttcgc gagcgtcatc
     2161 cgggcaattt acgggctaat acaccaccac cgcgcggatc gagcggccgg cgtgcatgag
     2221 atcgaacccc tcgttgatat ccgcgagctt gagcttgtgc gtgatcaggt cgtcgatgtt
     2281 gatcttgccc tccatgtacc agtcgacgat cttcggcacg tcgcgccggc ctttcgcgcc
     2341 gccgaacgcg gtgcccttcc atacccggcc ggtgacgagc tggaaggggc gcgtcttgat
     2401 ttcctggccg gcgccggcga cgccgatgat caccgacacg ccccagccgc ggtggcagca
     2461 ctcgagcgcc tggcgcatca cgtcgacgtt gccgatgcac tcgaagctgt agtccgcccc
     2521 gccgccggtc agctccacca ggtgcgccac caggtcgcct tgcacttcct tcgggttcac
     2581 gaagtgcgtc atgccgaact tctcccccag cgccttgcgc gcaggattca ggtcgacgcc
     2641 gatgatcttg ttcgcgccca ccatgcgcgc gccctgcacc acgttgagcc cgatgccgcc
     2701 gaggccgaac accaccacgt tggcgcccgg ctccaccttg gcggtgttga tgaccgcgcc
     2761 gatgcccgtg gtcacgccgc agccgatgta gcagaccttg tcgaagggcg catcctcgcg
     2821 g
//
LOCUS       DASUNT010000088         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_450170, whole genome shotgun sequence.
ACCESSION   DASUNT010000088 DASUNT010000000
VERSION     DASUNT010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450170"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1678
                     /locus_tag="VFX94_01805"
     CDS             32..1678
                     /locus_tag="VFX94_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5766942.1"
                     /translation="MLADALAAQGVQLAFGVPGESYLPVLDGLHDLQDRLRFVICRQE
                     GGASYMAEAYGKLTGEPGVLFVTRGPGATNGAIGVHTGLQDSTPMVVFIGQVPNEFSE
                     REAFQEIDYRRMYGQMAKWVGQIDRVERIPEYVSHAFHTAVAGRPGPVVLALPEDTLF
                     SQAAVADVPKYQVVRPAPDPSHIEQLKRLLEGAKQPFVLIGGGGWSREACIGLQEWVE
                     ANGLPVGTSFRCQDLFDNRSPNYAGDVGIGVNPKLAQRVKEADVLLVIGARLGEMTTS
                     AYSLVEVPVPQQTLVHVHAGAEELGRVYRPALPINSGMAQFVAALETLSFSSPSWKTR
                     TEQARKDFLEWTEPRPMPGQVQYGEVIRWLSERLPEDTIIAGGAGNFAGWLHRHFRYK
                     GFRTQLGSTNGSMGYGYPAAVAAKLARPQRTVFALCGDGDFLMTGQEIATAVQYGAHF
                     VTVVVNNGLYGTIRMHQEREYPGRVYGTELRNPDFAAYARAFGGHGETVERTADFAPA
                     FERAAAAGKPAIIELRIDPDAITPATTLSAIRDKALRSRQ"
     gene            complement(1662..>2000)
                     /locus_tag="VFX94_01810"
     CDS             complement(1662..>2000)
                     /locus_tag="VFX94_01810"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX5766943.1"
                     /translation="KCPAYCCSYPEIEVAPRDIERLAKHFGLPYSVAEERFTKYDAKE
                     KVRALRHRQDRIFATVCTFLDQKTRRCGVYEARPAVCREYPDSPRCGYYEFLKFERAH
                     QDDPKFIALT"
BASE COUNT          320 a          667 c          683 g          330 t
ORIGIN      
        1 gcccgacctc aacacccgcc atggcggccg gattctcgcc gatgcgctcg ccgcgcaagg
       61 cgtgcagctt gctttcggag tgcccgggga gagctacctc ccggtactcg acggcctgca
      121 tgacctgcag gaccggctgc gcttcgtcat ctgccgccag gagggcggcg cctcttacat
      181 ggccgaggcg tacggcaagc tcaccggcga accgggggtg ctcttcgtga cgcgcggacc
      241 gggcgcgacc aacggcgcga tcggcgtgca caccggcctg caggactcca cgccgatggt
      301 ggtcttcatc ggccaggtgc cgaacgagtt ttccgagcgc gaggcgttcc aggagatcga
      361 ctaccggcgc atgtatggcc agatggcgaa gtgggtcggg cagatcgacc gggtcgagcg
      421 catcccggaa tacgtgagcc atgccttcca cacggcggtc gcgggacgcc ccggcccggt
      481 ggtactcgct ctcccggaag acacgctttt ttcgcaggcg gctgtcgccg acgtgccgaa
      541 gtaccaggtc gtgcgtcccg cgccggaccc ttcgcacatc gagcaactga aacgacttct
      601 cgagggcgcg aagcagcctt tcgtgctcat cggcggcggg ggctggtcgc gcgaagcctg
      661 cattgggctg caggagtggg tcgaggcgaa cggcctgccg gtcggtactt cgtttcgctg
      721 ccaggatctc ttcgacaacc gaagccccaa ctacgccggc gacgtcggca tcggcgtcaa
      781 tccgaagctt gcgcagcgcg tgaaggaagc cgatgtactg ctcgtcatcg gcgcgcgcct
      841 cggcgagatg acgacgagcg cttactcgct cgtcgaagtg ccggtgccgc aacagacgct
      901 cgttcacgtg catgccggtg ccgaggagct cggccgggtc taccgtccgg cgctcccgat
      961 caactcgggg atggcccagt tcgtggccgc tctcgaaaca ctttctttca gcagcccttc
     1021 gtggaagacc cggaccgagc aggcgcgcaa ggatttcctc gagtggaccg agccccggcc
     1081 catgcccggc caggtgcagt acggcgaggt gatccgctgg ctatcggaac ggctgcccga
     1141 ggacaccatc atcgccggcg gcgccggcaa cttcgccgga tggctgcacc ggcatttccg
     1201 ctacaaaggc ttccgcaccc agctcggctc gacaaacggc tcgatgggct acggctatcc
     1261 ggcggcggtt gcggccaagc tcgcgcggcc gcagcgcacc gtgttcgcgc tttgcggcga
     1321 cggcgacttc ctcatgaccg ggcaggagat cgccaccgcc gtgcagtacg gcgcgcattt
     1381 cgtgacggtc gtggtgaaca acgggcttta cggcacgatc cggatgcacc aggagcgcga
     1441 gtatccgggc cgtgtctacg gtacggagct gcgcaatccg gacttcgccg cgtacgcgcg
     1501 cgcgttcggc ggtcacggcg agacggtgga gcgcactgcg gacttcgcac ccgccttcga
     1561 gcgcgccgcg gcggcgggca agccggccat catcgagctc aggatcgatc cggacgcgat
     1621 cacgccggcc acgacgctca gcgcgatccg ggacaaggcg ctcaggtcaa ggcaatgaac
     1681 ttcgggtcgt cctggtgggc gcgctcgaac ttcaggaact cgtagtagcc gcagcgcggg
     1741 ctgtcgggat attcgcggca aaccgccggc cttgcctcgt acacgccgca gcggcgcgtc
     1801 ttctggtcaa ggaaggtgca caccgtggcg aagatgcggt cctggcggtg gcgtagggcc
     1861 cgcacttttt ccttggcgtc gtacttcgtg aagcgctcct cggccacgct gtagggcagg
     1921 ccgaagtgct tcgccaggcg ctcgatgtcg cgcggcgcga cctcgatctc gggatagctg
     1981 cagcagtagg ccgggcactt
//
LOCUS       DASUNT010000089         3241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_450254, whole genome shotgun sequence.
ACCESSION   DASUNT010000089 DASUNT010000000
VERSION     DASUNT010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450254"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..>3241)
                     /locus_tag="VFX94_01815"
     CDS             complement(97..>3241)
                     /locus_tag="VFX94_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX5766944.1"
                     /translation="LKIALFPVPHGELRKLRLEIPATLSATVEEAQVDLRLWPLLRGS
                     AEVSSMVVRRPSLHLAGGAAAKEEPLDAVGAYRKVATALAQSLQRFAPDTALRIENAA
                     LDDFRNVNFSARTGAQGVDLEATGESRWWKRLAAKGRMEYADLATRAQIDVEGLVVDS
                     DVPSAELRVQLHAQQNAIRAEFHSRIVSLAAAQGKLVLADGAPAQATVHVSALDLAQA
                     LAIARRKVPGLDALESAAGQVWLTANAVLGGDWQAEIDVTKSSGALKLTQLPWAVVVQ
                     RAHVALTPHGIRVDKASGHVGASAFDDAHAQIELSDPRRLTAASAHARLDLEQWFPWL
                     AARLPLEAVDAMSGKVEVTLKRLALRFDRPQQADFDAVATPHNVSASLKALPGRLRMD
                     TGRVAVTPRRLGIAALRGSLGESKFTDIRAELELKPQPRVLSASGRASVELAQWLPML
                     RLDDVTTLTGKLDVALARLALRFDRPQDAHFEAIATPRDVSATLKMLPSPLALSGGSV
                     RVDRTTARIDEVAVRMLDAATRVSGTVTLGKPSADLVLSQGTLGEKAVRWTLERAGVP
                     ARFEPRTPLRFSAPRVAWAAQGALEADARIDFDGGPALGVALAWRPELFELRRLTLKD
                     ARTDAAFGATLAGELIQASFSGTLSGASIPAMLREPLPAGGVAKGAVRVTIDRKQPER
                     TQVEGKVAVEALDLTWLTGKKAVVERAEIVAERDGARIIGARFGWEDQFFELSGAGRR
                     TDEGPVIDARIVSDGVMLERLLPPADPNAPRKKSSALWPLPVSGRVELSAGFVQYKDY
                     RIEPLDGVLALEPNRARIEVKRARMCGVSFPTELEFEPESASAVAHVSIENEPLERTV
                     RCLTGGELDMTGTATLKAELRTVGRRPHLVRGLAGTVEADVRDGRVKKFALLGNILAF
                     RGIASLDDMKSDGFPYRRMTARGRFDAGRFLVEEAFFDSRAARLAASGHVDLLGPNSH
                     LSVLVAPLTTVERVVGAIPLVGDVFGGTMVALPIAVNGDIRNPAIVPLGPRAVTDQLL
                     GIFERTLKLPGKLVPAP"
BASE COUNT          495 a         1152 c         1124 g          470 t
ORIGIN      
        1 cgaagttgcg ctcgagcgcg agctccttgt cgagaagtgc ggggtcggcg gcgagaactt
       61 gccttgccag gagaagaagg actccgacca gcgctctcac ggcgccggca cgagcttgcc
      121 cggaagcttg agcgtgcgct cgaagatgcc gaggagctgg tcggtcacgg cgcgcggtcc
      181 gagcggcacg atcgccgggt tgcggatgtc gccgttgacc gcgatcggca gcgccaccat
      241 cgtgccgccg aacacgtcgc ccacgagcgg gatcgcgccg accacccgct ccacggtggt
      301 gagcggcgca acgaggacgc tgaggtgaga gttcggcccg agcaggtcca cgtggccgct
      361 cgcggcgagg cgcgccgcgc gcgagtcgaa gaacgcttct tccaccagga agcggccagc
      421 atcgaaccgt ccgcgcgccg tcatgcggcg gtaggggaag ccgtccgatt tcatgtcgtc
      481 cagcgaggcg atgccgcgaa acgccaggat gttgccgagg agcgcgaact tcttcacccg
      541 gccgtcgcgc acatcggcct ccaccgtgcc ggcgaggccg cgcacgaggt gcgggcgccg
      601 ccccacggtg cgcagctcgg ccttcagcgt cgcggtgccc gtcatgtcga gctcgccccc
      661 ggtcaggcag cgcacggtgc gctcgagcgg ctcgttttcg atgcttacgt gggcgaccgc
      721 ggaggccgac tcgggctcaa actccagctc cgtcgggaac gacacgccgc acatgcgcgc
      781 gcgcttcacc tcgatccgcg cccggttcgg ctcgagcgcg agcaccccgt cgagcggctc
      841 gatgcgatag tccttgtatt gcacgaagcc ggcggagagc tcgacgcgtc cgctgacggg
      901 cagcggccag agcgcggagg atttcttgcg cggcgcattc gggtcggcgg gcggcagcag
      961 ccgctcgagc atcacgccgt cggaaacgat gcgtgcgtcg atcacgggac cctcgtcggt
     1021 gcgccgcccc gcgccggaga gctcgaagaa ctggtcctcc cagccgaagc gcgcgccgat
     1081 gatgcgcgcg ccgtcgcgct ccgccacgat ctcggcgcgc tcgacgaccg ctttcttgcc
     1141 ggtgagccac gtgaggtcga gcgcctcgac ggcgaccttg ccttcgacct gcgtgcgttc
     1201 tggctgcttg cgatcgatcg tcacgcgcac tgcgcccttc gctacgccgc ccgccggcag
     1261 cggctcgcgc agcatggccg gaatgctcgc gcccgaaagc gtgccggaga agctcgcctg
     1321 gatgagctcg cccgcgagcg tggcgccaaa cgcggcgtcg gttcgcgcat ccttgagcgt
     1381 gaggcgccgc agctcgaaga gctcagggcg ccacgcgagc gctacgccga gtgccggacc
     1441 gccgtcgaag tcgatccggg catccgcttc cagagcgcct tgcgcggccc acgccacgcg
     1501 cggcgccgag aagcgcaacg gcgtcctcgg ctcgaagcgc gctgggacgc ccgcgcgctc
     1561 gagcgtccag cgcaccgcct tctcacccag cgtgccctgg gaaagcacca aatcggcgct
     1621 cggttttccg agcgtaacgg tccccgaaac ccgggtggcg gcgtcgagca tgcgcaccgc
     1681 gacttcgtcg attcgagcag tcgtgcgatc gacgcgaacc gaacccccgg aaagcgcgag
     1741 cggcgacggc agcatcttga gcgtcgcgct cacgtcgcgc ggcgtggcga tggcctcgaa
     1801 atgcgcgtcc tgcgggcgat cgaagcgcag cgcgagccgg gcgagcgcca cgtcaagctt
     1861 gccggtaagc gtagtgacgt cgtcgagccg cagcatcggc agccactgcg cgagctcgac
     1921 actcgcgcgc ccgctcgcgc tgagcacgcg cggttgcggc ttcaactcga gctcggcgcg
     1981 gatgtcggtg aacttcgact cgcccagaga cccgcgcagc gcagcgatgc ccaagcgccg
     2041 cggcgtgacc gcgacgcgcc cggtgtccat acgcagcctg ccgggcagcg ccttcagcga
     2101 cgcgctcacg ttgtgaggcg tggccacagc gtcaaaatcc gcctgctgcg ggcgatcaaa
     2161 gcgcagcgcg aggcgcttca gcgtgacttc gaccttgccg gacatcgcgt cgaccgcttc
     2221 tagcggcagc cgcgccgcca gccacggaaa ccactgctcg aggtcgagcc ttgcgtgggc
     2281 ggacgccgcc gtgagtcgcc taggatcgct cagctcgatc tgcgcgtgag cgtcgtcaaa
     2341 ggctgaagcg ccgacgtggc cgctcgcctt gtcgactcga atcccatgcg gggtgagcgc
     2401 cacgtgggcg cgctgcacga ccacggccca gggaagctgc gtaagcttga gcgcgccgct
     2461 cgacttcgtg acgtcgatct cggcctgcca gtccccgccg aggactgcat tcgcggtgag
     2521 ccagacctgt cccgcggccg attccagcgc atcgagcccc ggcaccttgc gacgcgcgat
     2581 ggcgagggct tgtgcgagat cgagcgcgct cacgtgcacc gtggcctgcg ctggcgcacc
     2641 gtcggcgagg acgagcttgc cctgtgccgc tgccagcgaa acgatgcggg agtgaaactc
     2701 agcccggatc gcgttttgct gcgcatgcag ctgcacgcgc agttccgccg acggcacgtc
     2761 gctgtcgacg acgagaccct caacgtcgat ttgcgcgcgc gtcgcgaggt ctgcgtattc
     2821 catgcggccc ttggccgcca gccgcttcca ccagcggctc tcgcccgtag cctcgaggtc
     2881 gacgccttgc gccccggtgc gggcggagaa gttcacattg cggaaatcgt cgagcgcggc
     2941 gttctcgatg cgcagcgccg tatcgggggc gaagcgctgc agcgactgcg ccagcgcggt
     3001 cgcgaccttg cggtaggcgc cgaccgcatc gagcggctcc tccttggcgg ctgcaccccc
     3061 ggcgagatgg aggctcggcc gtcgcaccac catggaggaa acttcggcgc ttccgcgcag
     3121 cagcggccag aggcgcagat cgacctgcgc ctcctccacc gtcgcgctaa gcgttgccgg
     3181 aatctcaagg cgcagcttgc gcagctcgcc atgcggcacc gggaagagcg cgatcttgag
     3241 c
//
LOCUS       DASUNT010000090         2801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_537861, whole genome shotgun sequence.
ACCESSION   DASUNT010000090 DASUNT010000000
VERSION     DASUNT010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537861"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /gene="ispF"
                     /locus_tag="VFX94_01820"
     CDS             complement(<1..272)
                     /gene="ispF"
                     /locus_tag="VFX94_01820"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX5766945.1"
                     /translation="MRIGQGFDVHPLVAGRKLVVGGVEIPYHKGLDGHSDADVLLHAV
                     CDALLGAAGLGDIGRHYPDTDSAYAGADSRRLLRDVARRLAAKKLKV"
     gene            complement(269..964)
                     /gene="ispD"
                     /locus_tag="VFX94_01825"
     CDS             complement(269..964)
                     /gene="ispD"
                     /locus_tag="VFX94_01825"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX5766946.1"
                     /translation="MSVYALIPAAGSGTRLSPGLPKQYLPLAGRPMLWHAVRALCVPP
                     VESVFVVLAPQDRMFATHDWSAFAGKLEPLYCGGETRRDSVYNGLVATMAAVEADDWM
                     LVHDAARPCLPRKDLEALLRETQDDEVGAILALPIADTVKRAAKDESGAQRVAATENR
                     SLLWLAQTPQMFRAGLLAQALRRAKGTPTDEAAAVEQMGLKPRLIAGSRENLKVTWAE
                     DLAMAESILKARA"
     gene            1014..>2801
                     /locus_tag="VFX94_01830"
     CDS             1014..>2801
                     /locus_tag="VFX94_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX5766947.1"
                     /translation="MHRDSLPARRRYTRLAGSADALALARLARAEKPLAVVTASALDA
                     QRLFDEIRWFAPELRACLLPDWETLPYDQFSPHQDLVSERLATLYRIQRGDFDVAMAP
                     AATALVRLSPPSYIAGHTFLIKAGEKLSLERLKEQLALAGYEHVTQVVSPGEVCFRGG
                     LIDLFPMGSALPYRLDLDDDVIDAVKTFDVDTQRTLYAVNEIRMLPAREFPLDDAGRA
                     RFRSRWRELFEGDPSKKQLYRDVSNGVPAAGIEYYLPLFFDSVATLFDYLPTACVLAL
                     HHDVADAVQEFWRDANSRYKMAGGDPDRPLLPPAQLFVPAEEFYVRAQAFPRIDIVGR
                     PDQDGEPLIATALPALAVERRAQDPLAALKHFVDNSGLRVLISAESPGRRETMASYFA
                     EYGLKPQACASFEDFITKHSKCMLGVSPLARGFALPGEGWAVITEAELYAGVVRQRGR
                     GAEKRTSVEGMLRDLSELKVGEPVVHEQHGIGRYQGLVSLDVGDGNTEFLLLEYEGAD
                     KLYVPVAQLGVIGRYSGAQPEEAPLHKLGSGQWEKAKARAAKQVRDTAAELLALYAKR
                     AARQGHSVTLRQHDLDAFAEGFGFEETPDQ"
BASE COUNT          419 a          991 c          925 g          466 t
ORIGIN      
        1 accttgagtt tcttcgcggc gagcctgcgc gccacgtcgc gcagcagcct tcggctgtcg
       61 gcacccgcgt aggcgctgtc ggtgtccggg taatgccggc cgatgtctcc caggcccgcg
      121 gcgccgagca gcgcgtcgca gaccgcgtgg agcagcacgt ccgcatcgga gtggccgtcg
      181 agtcccttgt gataggggat ttccacgccg ccgacgacga gctttcgccc cgcgacgagt
      241 ggatgcacgt cgaagccctg cccgatcctc atgcacgcgc tttcaggatg ctttccgcca
      301 tcgccagatc ctccgcccag gtcaccttga ggttctcgcg gcttcccgcg atcagccgcg
      361 gcttgaggcc catctgctcc accgcggcgg cttcgtcggt cggcgtgcct ttcgcgcgcc
      421 tgagcgcctg cgcgagcagg ccggcgcgga acatctgcgg cgtctgcgca agccacagca
      481 ggctgcggtt ctccgtagcc gccacgcgct gcgcgccgct ttcgtccttc gccgctcttt
      541 tgaccgtgtc cgcgatcggc agcgcgagga tcgcgccgac ctcgtcgtct tgcgtctcgc
      601 gcagcagcgc ctccaggtcc ttgcgcggca ggcacgggcg cgcggcatcg tgcaccagca
      661 tccagtcgtc cgcttcgacc gcagccatcg tcgccacgag cccgttgtac accgaatccc
      721 ggcgcgtttc cccgccgcag tagagcggtt cgagcttccc ggcgaacgca ctccagtcgt
      781 gcgtggcaaa catgcggtcc tgcggcgcaa gcaccacgaa cacagactcc accggcggca
      841 cgcagagtgc tcgcaccgca tgccagagca tcggccgccc cgccagcggc aagtattgct
      901 tgggcagccc gggcgagagg cgagtaccgc tcccggctgc cggaatgagc gcgtagaccg
      961 acacagaagt tcttttaatt caggacgata taattgcaca ggtcccgctt ctgatgcacc
     1021 gcgattcgct tcccgcccgc cgccgttata cgcggctcgc cggctccgcc gatgcgctcg
     1081 cgcttgcacg cctcgcgcgg gcggagaagc cgctcgcggt agtgaccgcg agcgcgctcg
     1141 acgcccagcg gctcttcgac gagattcgat ggttcgcgcc tgagctgcgg gcatgcctgc
     1201 tgccggactg ggagacgctg ccttacgacc agttctcgcc gcatcaggat ctcgtctcgg
     1261 agcgcctggc cacgctctac cgcatccagc gcggcgactt cgacgtcgcg atggcgccgg
     1321 ccgccacggc gctcgtgcgc ctctccccgc cctcctatat cgccgggcac acgttcctta
     1381 tcaaggcggg agagaagctc tcgctcgagc ggttgaagga gcagctcgcg cttgccgggt
     1441 acgagcacgt aacccaggtg gtgtcgccgg gggaggtctg cttccgtggc ggcctgatcg
     1501 acctctttcc gatgggctcg gcgctaccct accggctcga tctcgacgac gacgtgatcg
     1561 acgcggtcaa gaccttcgac gtcgacaccc agcgcacgct ctacgcggtg aacgagatcc
     1621 gcatgctgcc ggcgcgcgag ttccccctcg acgatgctgg acgggcccgc tttcgcagcc
     1681 gctggcgcga gctcttcgag ggcgacccgt cgaaaaagca gctctaccgc gacgtctcca
     1741 acggcgtgcc ggccgcgggg atcgagtact acctgccgct cttcttcgac tccgttgcaa
     1801 cgcttttcga ctatctgccg acggcgtgcg tcctcgcctt gcaccatgac gtggccgatg
     1861 cggtgcagga gttctggcgc gacgccaact cgcgctacaa gatggcgggc ggcgatcccg
     1921 accggccgct tctgccgccc gcgcagcttt tcgtcccggc ggaagagttc tacgtgcgcg
     1981 cgcaggcttt tccgcgcatc gacatcgtcg gtaggcccga tcaggacggc gaacccctga
     2041 tcgcgaccgc gcttcctgcg cttgccgtcg agcggcgggc gcaggatccg cttgcggcgc
     2101 tgaagcactt cgtcgacaac tcgggcttgc gcgtgctgat ctcggcggag tcacccggcc
     2161 ggcgagagac gatggcgagc tacttcgccg agtacggcct gaagccgcag gcgtgcgcga
     2221 gcttcgagga cttcatcacc aagcactcga aatgcatgct cggcgtctcg ccgctcgcgc
     2281 gcggcttcgc gcttcccggc gaaggctggg cggtgatcac cgaggcggag ctctacgccg
     2341 gcgtggtgcg ccagcgcggg cgcggcgccg agaagcgcac ctcggtcgag ggcatgctgc
     2401 gcgacctttc ggagctcaag gtcggcgagc cggtggtgca cgagcagcat ggaatcggcc
     2461 gctaccaggg gctggtcagc ctggacgttg gcgatggcaa caccgagttc ctgctgctcg
     2521 aatacgaagg cgcagacaag ctttacgtgc cggtggcgca gctcggggtg atcgggcgct
     2581 acagcggcgc gcagcccgaa gaggcgccgc tgcacaagct gggcagcggc caatgggaga
     2641 aagcgaaggc gcgcgccgcg aagcaggtgc gcgataccgc cgccgagctc ctcgcgctgt
     2701 acgcgaaacg cgccgcccgg cagggacact cggtgacgct caggcagcac gatctcgacg
     2761 ccttcgcgga gggcttcggc ttcgaggaga cgcccgacca g
//
LOCUS       DASUNT010000091         3990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_625711, whole genome shotgun sequence.
ACCESSION   DASUNT010000091 DASUNT010000000
VERSION     DASUNT010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625711"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="VFX94_01835"
     CDS             <1..1316
                     /locus_tag="VFX94_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5766948.1"
                     /translation="EVGYQLKDSGARAIVCQDILHEKVIKSGAALDLTVVTNVNEYLP
                     ALKRLFGKKTEVSGEVHWLQDLLKKYPAKPPAVAFDPKQDLAALPYTGGTTGNPKGVM
                     LTHYNLVAAQTMARNAFPVFEEGKEVILAFLPFFHIYGQVVIMLQGLCQGHLLVLFTS
                     PDTEAILEAMERYGATVFYGVPTLYEYLKDHKDTNKVNWRRLKLILSGADTLHESTTQ
                     GWTRRTGSSITEGYGLSETCATSHVNPLHRPKAGSFGCPIASMQAAVIDPESLEFVPP
                     NGTGELVLCGPNVMQGYWQRPDETARAFIDKGGVRWLRTGDLVRMDDEGYFHFYDRSK
                     DLIKYKGYSIFAKDVEDVLYAHPQVKAAGVIGVPDPAVGQRIKAIVVLQGDARGKLSE
                     DEIKTYCRQSLAEYKVPHIVEFRGELPKTDVGKVSRRELRDETKA"
     gene            1316..2089
                     /locus_tag="VFX94_01840"
     CDS             1316..2089
                     /locus_tag="VFX94_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5766949.1"
                     /translation="MARAFFAAEALTKRFGGLTAVNKVSFSLARDEIVGLIGPNGAGK
                     TTLLRLITKILRPDSGRILFNDEDITGLRPWDVVERGIAGTFQNTRPFRHLPIVANVM
                     VPLVAPRARRGGEWVKRIEAKAMDALEFVGISDMALEPASALSQGDLKRLEVARAIAT
                     EPELLLLDEPLGGLNPAESELLAKSIRRLHKGGRFGRLHSEGPAVLMIEHKLKELMSI
                     VDRVIVVDYGEIIADGSPAEVVKSPKVIEAYLGSEHAAA"
     gene            2076..2825
                     /locus_tag="VFX94_01845"
     CDS             2076..2825
                     /locus_tag="VFX94_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5766950.1"
                     /translation="MPLLEVKDLRVRYDKAQILNGVSLAVNEGELVGLVGPNGAGKST
                     LLRAISGLVRFEERMKRGSHGDIVLEGEVRFAGERIDRLAAHEIRTRGLVHCPERRRP
                     FRELTVEENLLAGAFLSKSKVATQRRLDHCFALFPRLAERKTQIAGTLSGGEQQMLAT
                     ARALMFEAKLLAIDEPSLGLAPRIRAELFAAIAKIHAEGVPVLLVEQEVAQVFRMAKR
                     NYVLSQGRIIAEGSGQSLMADETLRAGYLGL"
     gene            complement(2778..3854)
                     /locus_tag="VFX94_01850"
     CDS             complement(2778..3854)
                     /locus_tag="VFX94_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5766951.1"
                     /translation="MYWLRRGWAELRNEVFVLPSRTLVLVWAIGLLLLPLVYGDPYVL
                     RIVTMTCIFAIFAASWDLLAGYTGQVNFGHALFFGAGAYTSALMSLKLGLSPWATIWA
                     GAAVAFLFGFVVGYLCLRLRGSYLSLATLAFPLIALGVLFAFPDFSGGELGISGLRRL
                     MVTPLSNYYVVAISMLVIVFALWAFADSRFGIVLHAIRDDEVAARASGINTLRYKLLV
                     FAIAGAAAGFAGALFAHYLRVAGPSTLEVALSFQVVIWGIFGGVATIYGPVAAVFILY
                     PLTEWLGSFRAFGELRLLIFAVIVLLVLLFMPRGLTPWVRDKIETQCPRCKQRNAAWK
                     KMCRLCGAGLEAQVPGAQRLVGHQ"
BASE COUNT          794 a         1289 c         1323 g          584 t
ORIGIN      
        1 atgaagtggg ctaccagctg aaggacagcg gcgcgcgcgc gatcgtctgc caggacatcc
       61 tgcacgagaa ggtcatcaag agcggagcgg cgctcgacct gacggtcgtc accaatgtca
      121 acgaatacct gccagcgctg aagcgtcttt tcggcaagaa aaccgaggtt tccggcgagg
      181 tgcactggct gcaagatctg ctcaagaagt accccgccaa gccgcccgcg gtcgcgttcg
      241 accccaagca ggacctggcc gctctgccgt atacgggcgg aaccaccggt aacccgaaag
      301 gcgtgatgct cacgcattac aacctggtgg ctgcgcaaac gatggcgcgg aacgcttttc
      361 cggttttcga ggagggaaaa gaggtgatcc tcgccttcct gccgttcttc cacatctacg
      421 ggcaggtcgt gatcatgctg caggggcttt gccagggaca cctgctcgtg cttttcacca
      481 gccccgatac cgaggcgatt ctcgaggcga tggaacgata cggcgccacc gtgttctatg
      541 gcgtgccgac gctgtacgaa tacctgaagg atcacaagga cacgaacaag gtgaactggc
      601 gccggctcaa gctcatcctt tcgggcgccg acacgctgca cgagtcgacc acccaagggt
      661 ggacgcggcg caccggatct tccattacag agggctacgg cttgtccgaa acctgcgcca
      721 ccagccacgt caacccgttg caccggccga aggccggctc tttcggctgc ccgatcgcta
      781 gcatgcaggc cgcggtgatc gacccggagt cgctcgagtt cgtgccgccg aatgggaccg
      841 gcgagctcgt cctgtgcggg ccgaacgtga tgcagggata ctggcagcgg cccgacgaaa
      901 ccgcgcgggc cttcatcgac aaggggggcg tacggtggct gcgcaccggc gatctggtgc
      961 gcatggacga cgagggctat ttccacttct acgaccgctc gaaggacctg atcaagtaca
     1021 agggctattc gatcttcgcg aaggacgtgg aggacgtgct ttacgcccac ccgcaggtaa
     1081 aggcagccgg cgtcatcggg gtgcccgacc cggcggtcgg gcagcgcatc aaggcgatcg
     1141 tcgtcctgca gggcgacgcc cgcggcaagc tctcggagga cgagatcaag acgtattgcc
     1201 gccagagcct agccgagtac aaggtgccgc acatcgtcga gttccgcggc gagctgccga
     1261 agaccgacgt cggcaaggtg tcgcgccgcg agctgcgcga cgagacgaag gcctagtggc
     1321 gcgagccttc ttcgccgccg aagcgctgac caagcgcttc ggcggcctca cggcggtcaa
     1381 caaggtcagc ttctcgctcg cgcgcgacga aatcgtgggc ctcatcgggc caaacggcgc
     1441 gggcaagacg acgctgctac gcctgatcac caaaatcctc aggcccgact cggggcgcat
     1501 ccttttcaac gacgaggaca tcacagggct gcgtccctgg gacgtagtcg agcgcggcat
     1561 cgcgggcacc ttccagaaca cgcggccgtt ccgtcacctg ccgatcgtcg cgaacgtcat
     1621 ggtgccgctg gtggcgccgc gcgcgcggcg cggcggcgaa tgggtgaagc gcatcgaggc
     1681 gaaggcgatg gacgcgctgg agttcgtcgg catctcggac atggcgctcg agcccgcctc
     1741 ggcactctcg cagggagact tgaagcgcct ggaggtcgcg cgcgccatcg ccacggagcc
     1801 cgagctgctg ctcctcgacg agccgctcgg cgggctgaac ccggcggagt cggagctgct
     1861 cgcgaagtcg atccgccgcc tgcacaaggg cgggcgcttc ggccggcttc attccgaggg
     1921 cccggcggtg ctcatgatcg agcacaagct gaaagagctc atgtcgatcg tcgaccgcgt
     1981 gatcgtggtc gactacggcg agatcattgc cgacggctcg cccgccgagg tcgtgaagag
     2041 tccaaaagta atcgaagcgt acctcgggag cgagcatgcc gctgcttgag gtgaaggacc
     2101 tgcgcgtgcg ctacgacaag gcgcagatcc tgaacggcgt gtcgcttgcg gtgaacgaag
     2161 gggagctcgt cggcctggtt ggaccgaacg gcgccggcaa atcgacgctg ctgcgcgcga
     2221 tctcgggcct ggtgcgcttc gaggagcgca tgaagcgcgg cagccacggc gacatcgtgc
     2281 tcgaaggcga agtgcgcttc gccggcgagc gcatcgaccg cctcgccgcg cacgagatcc
     2341 gcacgcgcgg cctcgtgcac tgccccgagc gccggcggcc gtttcgcgag ctcacggtcg
     2401 aggaaaacct gctcgccggc gccttccttt cgaagagcaa ggttgcgacg caacgccggc
     2461 tcgatcactg ctttgcgctc ttcccgcgcc ttgccgaaag gaagacgcag atcgccggaa
     2521 cgctatccgg cggcgaacaa cagatgctcg cgaccgcgcg cgcgctaatg ttcgaggcga
     2581 agctgctcgc gatcgacgag ccttcgctcg gccttgcgcc gcgaatccgc gcggaactct
     2641 tcgccgcgat cgccaagatc cacgccgagg gggtgccggt gctgctggtc gagcaggaag
     2701 tggctcaggt attccggatg gctaagcgca actacgtcct ctcccagggc cgcatcattg
     2761 ccgagggctc ggggcagtca ctgatggccg acgagacgct gcgcgccggg tacctgggcc
     2821 tctagcccgg cgccgcacaa ccggcacatc ttcttccagg cagcatttct ttgcttgcag
     2881 cgcgggcact gcgtttcgat cttgtcgcgc acccacggcg taagcccgcg aggcatgaac
     2941 agcagcacga gcagcacgat gaccgcgaag atgagcaggc gcagctcgcc gaaagcgcgg
     3001 aagctgccga gccactcggt gagcgggtag aggatgaaca ccgcagcgac cggtccatag
     3061 atagtcgcga cgccgccgaa gatgccccag atcaccacct ggaaggaaag cgcgacttcg
     3121 agcgttgaag gcccggcgac gcgcagataa tgggcgaaga gcgcgccggc gaagcccgcg
     3181 gccgcaccgg cgatggcgaa cacgaggagc ttgtagcgca gggtgttgat gccggaggcg
     3241 cgtgccgcca cctcgtcatc gcgtatggcg tgcagcacga tgccgaatct ggaatcggcg
     3301 aaagcccaga gcgcaaacac gatgacgagc atcgagatcg ccacgacata gtaattgctc
     3361 aggggcgtga ccatcagccg gcgtaatccc gagatgccga gctcgccgcc ggagaaatcc
     3421 gggaaggcga acagcacccc gagcgcgatc agcgggaagg ccagcgtggc gagcgaaaga
     3481 tacgagccgc gcagccgcag gcagaggtag ccgacgacaa agccgaatag aaatgccacc
     3541 gccgcgcctg cccagatcgt ggcccagggc gagagaccga gcttgagcga catgagcgcc
     3601 gaggtgtacg cgcccgcgcc gaagaacagc gcatggccga agttcacctg cccggtatag
     3661 ccggcgagca gatcccagct cgccgcgaag atcgcgaaga tgcacgtcat cgtgacgatt
     3721 cgcagaacgt acggatcgcc atacacgagg gggagcagaa gaagcccgat cgcccagacc
     3781 agcacgagcg tgcgcgaagg aagcacgaac acctcgttgc gcagctccgc ccatccgcgc
     3841 ctgagccaat acatcagcgt tcctcctcga acgccacgcc gaagaggccc tcgggccgtg
     3901 caagcagcac cgcgaccatg atcatgagcg cgacagcgcc cttgagatat gcgccggcgg
     3961 gcaccgcgaa cacggtgatc gcctcgacgt
//
LOCUS       DASUNT010000092         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_275570, whole genome shotgun sequence.
ACCESSION   DASUNT010000092 DASUNT010000000
VERSION     DASUNT010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275570"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VFX94_01855"
     CDS             complement(<1..794)
                     /locus_tag="VFX94_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEX5766952.1"
                     /translation="MNISVEAKGGSVVLGSGTRRYRVVENWAKLPEGWEFKDVAAVAV
                     DSKDRVYVFNRGEHPMIVFDRDGSFIRAWGEGTFPRAHGLHIDTNDILYCTDDGGHIV
                     RKCTTEGKVLLELGVPGKPAPYMSGTPFHRCTHTALSPRGDIYVSDGYGNARVHKFSP
                     DGKLLKSWGEPGTDPGQFNIVHNIVTDPEGWVYVADRENHRVQVFDGEGRYETQWVNM
                     HRPCALYCCGGPHPEFVIGELGPSMPINRQHPNIGPRLSFVDKKGKL"
     gene            855..1997
                     /locus_tag="VFX94_01860"
     CDS             855..1997
                     /locus_tag="VFX94_01860"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX5766953.1"
                     /translation="MKARNRRLLGAAVAAVLAASALQVNAQGTPRTLKMQASWPSTVT
                     AWDNFRFVAERIDKTTGGAIKIETLAAGQIVPAFEVLDATHKKVIDGAHSIAYYWVGK
                     NKTAVLFTGGPGGPFGMDMIDFLGWMYEGGGWDLYREFYQDVLKLNVIAFPTLPTSPQ
                     AFGWFKRPVKNLADFKGMKCRQTGITAEVFQRMGMSTVNMPGGEIIPAAQRGTIDCAE
                     WVGGKDDLNLGFHSVWKYHYVPGMHESTSTGELIINGDVWASFTPQQQEIVRSVTNES
                     FARWWPRWQKGQADAMKELQEKHGVRILRTPPDILTAFLKAWDEVAAEESAKNPFFKK
                     VLDSQRAYASVVVPAKRFLFPPYSFAANYYWPEKPAGDAKKAAAAK"
     gene            2026..2310
                     /locus_tag="VFX94_01865"
     CDS             2026..2310
                     /locus_tag="VFX94_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5766954.1"
                     /translation="MKSFEAFAAEHRAAGADEVLERRWQPNTVIDTHTHPFAAHALVV
                     QGEMWLSIGDSTRHLRPGDRFELEADVAHAERYGAEGATYWVARRNARKS"
     gene            complement(2307..>2911)
                     /locus_tag="VFX94_01870"
     CDS             complement(2307..>2911)
                     /locus_tag="VFX94_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX5766955.1"
                     /translation="PGVKVKVGKAEAREDEARVAAVRRLIGSRVDLMLDANMGWNADK
                     ALERGRRLEQFGLYWYEEPTIPEDVAGHARLARELDVPIALGESLHSPHEFRRYIEER
                     AVEVVQIDPVTNGGITASAHALEMGNAAGLKASSHYCDELAAHLLCAATEPVYLEKHA
                     FALDRYLEAPQEVRNGRVRPTETAGTGMRFSEKALARYRG"
BASE COUNT          487 a          963 c          982 g          479 t
ORIGIN      
        1 agcttgcctt tcttgtcgac gaaagacagg cgcgggccga tgttggggtg ctggcggttg
       61 atcggcatgg acggaccgag ctcgccgatc acgaattccg gatgcggtcc gccgcagcaa
      121 tagagcgcgc acgggcgatg catgttcacc cactgcgtct cgtatcgtcc ttcgccgtcg
      181 aacacctgca cgcggtggtt ctcgcggtcg gcgacgtaga cccagccttc cgggtcggtg
      241 acgatgttgt gcacgatgtt gaactgaccc ggatcggttc ccggctcgcc ccacgacttg
      301 aggagcttgc cgtcgggcga gaacttgtgc acgcgcgcgt tgccgtagcc gtcgctcaca
      361 tagatatcgc cccgcggcga aagcgcggta tgggtgcagc ggtgaaacgg cgtgccgctc
      421 atgtacggcg cgggcttccc cggcacgccg agctcgagca ggacctttcc ttcggtggtg
      481 cacttgcgca cgatatgccc gccgtcgtcc gtgcagtaca ggatgtcatt cgtgtcgatg
      541 tgcaggccgt gcgcgcgcgg gaaggtgccc tcgccccacg cgcggatgaa gctgccgtcg
      601 cgatcgaaga cgatcatcgg gtgctcgccg cgattgaaga cgtacacccg gtccttgctg
      661 tccaccgcga ccgccgccac gtccttgaac tcccagccct ccggcagctt cgcccagttc
      721 tccaccacac ggtagcggcg ggtgccgctg ccaaggacca ccgagccgcc tttcgcttca
      781 acgctgatgt tcatgacgcc tttctaccac gagggagtag actgcagagg gtttacttgc
      841 cggaggagga gcccatgaaa gcacgcaatc gccgcctgct cggcgcggcc gtcgcggccg
      901 tcctcgccgc gagcgcgctg caggtgaacg cgcaaggcac gccccgcacg ctgaagatgc
      961 aggcctcgtg gccgagcacg gtgaccgcct gggacaactt ccgcttcgtc gccgagcgca
     1021 tcgacaagac gaccggcggg gcgatcaaga tcgaaaccct cgccgccggg cagatcgtgc
     1081 ccgccttcga ggtgctcgac gcgacgcaca agaaggtgat cgacggcgcg cactcgatcg
     1141 cctactactg ggtcggcaag aacaagacag cggtgctctt caccggcggt ccgggcggcc
     1201 cgttcggcat ggacatgatc gacttcctcg gctggatgta cgagggcggc ggctgggacc
     1261 tctaccgcga gttctaccag gacgtgctca agctcaacgt catcgccttc cccacgctgc
     1321 cgacgagccc gcaggccttc ggctggttca agcggccggt caagaacctc gccgacttca
     1381 agggcatgaa gtgccggcag accggcatca ccgcggaggt gttccagcgc atgggcatga
     1441 gcacggtcaa catgcccggc ggcgagatca tcccggcggc gcagcgcggc acgatcgact
     1501 gcgccgaatg ggtcggcggc aaggacgact tgaacctcgg cttccactcg gtgtggaagt
     1561 accactacgt gccgggcatg cacgagtcca cctcgaccgg tgagctgatc atcaacggcg
     1621 atgtgtgggc gtctttcacg ccgcagcagc aggagatcgt gcgctcggtg acgaacgagt
     1681 cgttcgcgcg ctggtggccg cgctggcaga aggggcaggc cgatgcgatg aaggagctgc
     1741 aggagaagca cggcgtgcgc atcctgcgca ccccgcccga catcctcacg gcgttcctca
     1801 aggcgtggga cgaggtcgcg gccgaggagt cggcgaagaa cccgttcttc aagaaagtgc
     1861 tcgactcgca gcgcgcttac gcctcggtgg tggtgccggc gaagcgcttt ctcttcccgc
     1921 cctactcctt cgcggcgaac tactactggc ccgagaagcc ggccggcgat gcgaagaagg
     1981 cggcggcggc gaagtgagcc gggcgatcga ctcgaggagc agcacatgaa aagcttcgaa
     2041 gcattcgccg ccgagcaccg cgctgcgggc gcggacgagg tgctggaacg gcgctggcag
     2101 cccaatacgg tgatcgacac gcatacgcac ccgttcgcgg cgcacgctct cgtggtgcag
     2161 ggggagatgt ggctaagcat cggcgatagc actcggcacc tgagacccgg cgatcgcttc
     2221 gagctcgagg ctgacgtggc ccacgccgag cgctacggcg ccgagggcgc cacttactgg
     2281 gtggcaaggc gtaacgcgag gaaaagctag cccctgtaac gcgcgagcgc cttttcggaa
     2341 aagcgcatcc cggttcccgc ggtctcggtc ggacgcacgc ggccgttcct gacctcctgc
     2401 ggggcctcga ggtagcggtc gagcgcgaag gcatgcttct cgaggtacac gggctcggtc
     2461 gccgcgcaga gcaggtgcgc ggccagctcg tcgcaatagt ggctgcttgc cttgaggccg
     2521 gcggcgttgc ccatttccag ggcgtgcgct gaagcggtga tgccgccgtt ggtgaccggg
     2581 tcgatctgga ccacctcgac ggcgcgctcc tcgatgtagc gacggaattc gtgtggcgag
     2641 tgcaggctct cgccgagcgc aatcggcacg tcgagctcgc gcgcgaggcg cgcgtgcccg
     2701 gcgacgtcct cggggatcgt cggctcctcg taccagtaga ggccgaactg ctccaggcga
     2761 cggccgcgct cgagcgcctt gtctgcattc cagcccatgt tggcgtccag catgaggtcc
     2821 acgcggctgc cgatcagcct gcgcacggcc gccacgcgcg cctcgtcctc gcgcgcttcc
     2881 gccttgccga ccttgacctt cacgccggga a
//
LOCUS       DASUNT010000093         2809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_977375, whole genome shotgun sequence.
ACCESSION   DASUNT010000093 DASUNT010000000
VERSION     DASUNT010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977375"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1348)
                     /gene="dnaA"
                     /locus_tag="VFX94_01875"
     CDS             complement(26..1348)
                     /gene="dnaA"
                     /locus_tag="VFX94_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX5766956.1"
                     /translation="MHNLWDACLRRLEQELPAQQFNTWIRPLAPEAAEAADTLVLAAP
                     NRFVLELVRERFAARIERLASEASGRDLGLRLVLARIAEPAAPQPVPRAAETPSILER
                     ARLNPAFSFDSFVTGKANQLARAAALQVAENPGVSYNPLFVYGGVGLGKTHLVQAIGN
                     QLADQRPQARIRYIHAEQYVTDVVRAYQQKSFDEFKRYYHSLDLLLIDDIQFFSNKGR
                     TQEEFFYAFNALVEAHKQIVITCDTYPKEIAGMEERLISRFGWGLTVAIEPPELEMRV
                     AIVLKKAEAESVALSEDIAFFIAKHLRSNVRELEGALKKVLAFARFSNRELSLELAKE
                     ALQDILSVASRQVSVEGIQKTVAEYFKLKISDMHSKKRSRNVARPRQVAMALAKDLTQ
                     MSLPEIGEAFGNRDHTTVLHACRTIASLRKHDTALNRDYVVLQQVLKG"
     gene            1467..1607
                     /gene="rpmH"
                     /locus_tag="VFX94_01880"
     CDS             1467..1607
                     /gene="rpmH"
                     /locus_tag="VFX94_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX5766957.1"
                     /translation="MATKRTYQPSKTRRARRHGFLVRSRTAGGRKVLRNRRARGRRRL
                     GL"
     gene            1649..1954
                     /gene="rnpA"
                     /locus_tag="VFX94_01885"
     CDS             1649..1954
                     /gene="rnpA"
                     /locus_tag="VFX94_01885"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX5766958.1"
                     /translation="MRQKADFERLLRHGARRNLDGYTMYVGRRDSGAARLGLLISRRH
                     APRAVVRNRIKRCIREAFRLEQERLGPMDVLVRPPYGVKTSAQMVVRLRELFGRLVQ"
     gene            1951..2163
                     /gene="yidD"
                     /locus_tag="VFX94_01890"
     CDS             1951..2163
                     /gene="yidD"
                     /locus_tag="VFX94_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX5766959.1"
                     /translation="MTVRALCFLLVAYRWCVRPLLPSACRFYPSCSCYAEEALLRHGF
                     WRGGWLAARRVCRCGPWHSGGHDPVP"
     gene            2167..>2809
                     /gene="yidC"
                     /locus_tag="VFX94_01895"
     CDS             2167..>2809
                     /gene="yidC"
                     /locus_tag="VFX94_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX5766960.1"
                     /translation="MDTQRLILLVIFSFSLLMLWEAWEKEKRPKPPPTAPAEQPGVPA
                     PAKPAPPAAKPSPAVPGAEAAKGEVIRVSTDLVVAEIDTLGGTLKRVELVRHKDSKDL
                     SKNLVLLGSERQYEAQSGLAGEGGPNHRTLWRAEPGERALAPGRDEVAVQLVAAGKDG
                     LEVRKSYTFKRSSYVVDVVLDVRNPGAAPVAAYAYFQLTHDGKTETNTTTVAET"
BASE COUNT          465 a          926 c          928 g          490 t
ORIGIN      
        1 cccaaggtcc cccacagctt tcccgtcagc ctttcagcac ctgctgcagc acgacgtagt
       61 cgcggttgag cgccgtgtcg tgcttgcgca ggctggcaat ggtgcggcag gcatgcagca
      121 ccgtggtgtg gtcgcggttg ccgaacgctt cgccgatctc gggcaggctc atctgcgtga
      181 ggtccttggc gagcgccatg gccacctggc gcggccgcgc cacgttgcgg ctgcgtttct
      241 tggagtgcat gtccgaaatc ttgagcttga aatactcggc cacggtcttc tggatgcctt
      301 cgacgctcac ctggcggctc gccacgctga ggatgtcctg cagcgcctcc ttggcgagct
      361 cgagcgagag ctcgcggttg ctgaagcggg cgaacgccag caccttcttg agcgcgccct
      421 ccagctcgcg cacgttggag cgcaggtgct tggcgatgaa gaaagcgatg tcctcggaca
      481 gcgcgaccga ctcggcctca gccttcttca gcacgattgc cacgcgcatc tcgagctccg
      541 gcggctcgat cgctacggta agaccccagc cgaagcgcga gatcagccgc tcctccatgc
      601 cggcgatctc cttggggtag gtatcgcagg taatcacgat ctgcttgtgt gcctccacca
      661 gcgcgttgaa ggcgtaaaag aactcttcct gagtccgtcc cttgttggag aagaactgga
      721 tatcgtcgat cagcagaagg tcgagcgagt ggtaatagcg cttgaactcg tcgaacgact
      781 tctgctggta agcccgcacc acgtcggtga cgtactgctc ggcatggatg tagcggatcc
      841 gcgcctgcgg ccgctggtcg gcgagctggt tgccgatcgc ctgcaccagg tgcgtcttgc
      901 cgagtcccac gccgccgtag acgaaaagcg ggttatagga aaccccggga ttttccgcga
      961 cctggagcgc ggcggcgcgc gcaagctggt tcgcctttcc ggtgacgaag ctgtcgaacg
     1021 agaacgccgg attgagcctg gcgcgctcaa gaatcgacgg cgtctcggcg gcacgcggga
     1081 cgggttgcgg cgccgcaggc tccgctatgc gcgccagaac gaggcgcaag ccgaggtcgc
     1141 gcccgctcgc ctcggatgcc agacgctcga tgcgcgcggc gaagcgctcg cgcaccagct
     1201 ccagcacgaa ccgattcggt gccgccagga ccaacgtgtc ggccgcctcg gccgcctccg
     1261 gcgcaagcgg ccggatccac gtgttgaact gttgcgcggg gagctcctgc tcgaggcgcc
     1321 gcaggcaggc atcccagagg ttatgcatct tttcgttggg ccgaaagcga atcggaattt
     1381 taccttctta ggcatgccat atccacagac gaattgacaa tgcgcgcgcg cgcgcgctct
     1441 aataccgccc tttttcggac cggcagatgg cgacgaagcg gacttaccaa ccttccaaga
     1501 ctcggcgcgc gcggcgccat ggttttctcg tgcgcagccg cactgccggc ggccgcaaag
     1561 tgctgcgtaa ccggcgcgct cgcggccggc gccggctcgg gctgtagccg cggccggcaa
     1621 gcgcttcggg ctggatgccg cacgccggct gaggcaaaag gccgacttcg aacggctgct
     1681 gcgacacggc gcgcgacgta acctggacgg ctacaccatg tatgtggggc gacgcgacag
     1741 cggagcggcg cggcttggcc tcctgatctc gcgcaggcac gcgccgcgcg ccgtcgtgcg
     1801 taatcgcatc aagcgttgca tccgcgaggc tttccgcctg gagcaggagc ggctcggccc
     1861 tatggatgtg ctagtgcgcc cgccttacgg tgtaaagact tccgcgcaga tggtcgtccg
     1921 cctgcgcgag ctttttggca ggctcgtcca atgacggtgc gcgcgctgtg cttcctgctg
     1981 gtcgcttatc gctggtgcgt ccggcctttg ctcccgtccg cgtgccgttt ctacccgagc
     2041 tgctcatgct acgcagagga agctttgctg cgacacgggt tctggcgcgg cggctggctc
     2101 gcggcgcggc gcgtatgccg ctgcggcccc tggcattccg gcggtcacga tcccgtcccg
     2161 taggccatgg atacgcaaag gctgatcctc ctcgtaatct tcagcttctc gcttctcatg
     2221 ctttgggaag catgggagaa ggagaagcgc ccgaagccgc cacccaccgc gccggcggag
     2281 cagccgggcg tgcccgcgcc ggccaagccg gcgccgccag ccgccaaacc ctcgcctgct
     2341 gtccccggcg ccgaagcggc aaagggcgag gtcatacgcg tgagcacgga cctcgtcgtg
     2401 gccgagatcg acacgctcgg agggacgctg aagcgtgtgg agcttgtgcg ccacaaggat
     2461 tccaaggacc tttccaagaa cctcgtccta ctgggctccg agcgtcagta cgaagcgcaa
     2521 agcggcctcg ccggcgaagg cggacctaac caccgcacgc tgtggcgcgc cgagccggga
     2581 gagcgcgcgc tcgccccagg ccgcgacgag gtagcggtac agctcgtcgc cgctgggaag
     2641 gacgggctcg aagtgcgcaa gagttacacc ttcaagcgaa gcagctatgt cgtcgacgtg
     2701 gtgctggacg tgcgcaatcc cggtgccgcg ccggtggccg cctatgcgta cttccagctc
     2761 acgcacgacg gcaagacgga aacgaacacg actacggtcg ccgagactt
//
LOCUS       DASUNT010000094         2305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1153652, whole genome shotgun sequence.
ACCESSION   DASUNT010000094 DASUNT010000000
VERSION     DASUNT010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153652"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VFX94_01900"
     CDS             complement(<1..730)
                     /locus_tag="VFX94_01900"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX5766961.1"
                     /translation="MSTETAIGLAMLGVMVAAIFLGFPISFTLLFLAFGFGWWGLGDV
                     VFDLAYFQTIGLMKESLLAAVPLFIFMGFVAEQAGLMERLFIALRLVLAPVRGSLFIV
                     VIVTAAAFAMATGIVGAAVTVLGIMAAPIMMRAGYDGRLAAGAITAGGTLGILIPPSV
                     MLIVMGPVLGVSVADLYAAAIVPGFMLAGMYVVYVAARSWWNPKLGPALPKEERAPSV
                     WSALGAFVVGVVPLAGLVAATLGSI"
     gene            complement(727..1242)
                     /locus_tag="VFX94_01905"
     CDS             complement(727..1242)
                     /locus_tag="VFX94_01905"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX5766962.1"
                     /translation="MRLRPLVRIIDRFAEVTGVAIAWLMVPLIGAVVYEVVARYVFAA
                     PTLWAFDITYMLYGAMFMLGAAYALRVGAHIRTDFFWERWSARTRGVIDTVAYLVFFF
                     PGIALFLWVGWEEAWYAYGIGETSEQTAWRPILWPLKACIPLAAALLLLQGISELVKS
                     LHAALTGRAFA"
     gene            1307..1744
                     /locus_tag="VFX94_01910"
     CDS             1307..1744
                     /locus_tag="VFX94_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766963.1"
                     /translation="MDARTAASRIVAAVSPGVRERICAIAPRGELCFVHTAEALLREL
                     DNGCDMLILGLHFDESTMVAALQRVRARAEAFPVVCVRGRPSRLGQRSLSALRMALNE
                     LGAQNFIDLLEYPDDEAGNARVRATLERLLQDGPLGAAHRVRP"
     gene            1780..2187
                     /locus_tag="VFX94_01915"
     CDS             1780..2187
                     /locus_tag="VFX94_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766964.1"
                     /translation="MCAPQGAARRVVAAVTDDLQPRIRAIFPGCDVRFVRSGTELVRA
                     LDEARCEMMVVQVHFNESSAAAALMCVLAREESFPVVCVRDVPDAKPAYAALNALRIA
                     LGGIVARAFIDLVEHRDDEAGNARVRAMLEPLL"
BASE COUNT          422 a          771 c          805 g          307 t
ORIGIN      
        1 ggatcgagcc gagcgtcgcg gcgaccaggc cggcgagcgg caccacgccg acgacgaagg
       61 cgccgagcgc cgaccaaacg gacggggcgc gctcctcctt gggcagcgcg ggcccgagct
      121 tcgggttcca ccagctgcgc gcggcgacgt acaccacgta catgccggcc agcatgaacc
      181 cgggcacgat ggcagcggca tagaggtcgg cgaccgagac accgagcacg gggcccatca
      241 cgatcagcat caccgaagga ggaatcagga tgccgagggt gccgcccgcg gtgatcgcgc
      301 ccgcggcgag ccggccgtca tagcccgcgc gcatcatgat cggcgcggcc atgatgccga
      361 gcacggtgac tgcggcgccg acgatgccgg tcgccatcgc aaaggcggcg gcggtgacga
      421 tcacaacgat gaagagcgag ccgcgcaccg gcgcgagcac gaggcgcagc gcgatgaaga
      481 gccgctccat caggccggcc tgctcggcga cgaagcccat gaagatgaaa agcggcaccg
      541 ccgcgagtag cgactccttc atgagcccga tggtctggaa gtacgcgagg tcgaaaacga
      601 cgtcgcccag gccccaccag ccgaagccga aggcgagaaa aagcagcgtg aaagagatcg
      661 ggaagccgag gaaaatcgcc gcgaccatga caccgagcat cgccaggccg atcgcggtct
      721 cggtgctcac gcgaatgccc gtccggtgag cgcggcgtgc aggcttttca cgagctcgga
      781 gattccctgc aggaggagga gcgcggcggc gagcgggatg caagccttga gcggccacag
      841 gatcggccgc caggcggtct gctcggaagt ctcgccgatg ccgtaggcgt accaggcttc
      901 ttcccagccc acccacagga agagcgcgat gccggggaag aagaacacga ggtaggcgac
      961 ggtgtcgatc acgccgcgcg tgcgcgccga ccagcgctcc cagaagaaat cggtgcggat
     1021 atgggcgccc acgcgcagcg cgtaggccgc gccgagcatg aacatcgcgc cgtacagcat
     1081 gtaggtaatg tcgaaggccc acagcgtcgg cgcggcgaat acgtagcgcg cgaccacctc
     1141 gtacaccacc gcaccgatga gcgggaccat cagccaggcg atcgccacgc ccgtgacctc
     1201 ggcgaagcgg tcgatgatgc gcaccagagg ccgcaggcgc atggtgccga gactaggaac
     1261 cgaagactta agtagccttt aagccgggcg gcggcagaat gcaggcatgg acgcgcggac
     1321 cgcagcgtcg cggatcgtgg ccgcggtcag cccaggggtc cgggagcgca tttgcgccat
     1381 cgccccccga ggcgagctgt gcttcgtcca tacggcggag gcactgctgc gcgagctcga
     1441 caacggctgc gacatgctga tcctcggctt gcacttcgac gaatccacca tggtggcggc
     1501 gctgcagcgc gtgcgggcgc gcgcggaggc gttcccggtc gtgtgcgtgc gcggcaggcc
     1561 gtcgcgcctc ggacagcgtt cgctgtccgc gctgcgcatg gcgctgaacg aacttggcgc
     1621 gcagaacttc atcgacctcc tggaatatcc cgacgacgaa gccggcaacg cgcgcgtgcg
     1681 cgccacgctc gagcgcctgc tccaggatgg tccgctcggc gcagcccacc gagtgcgtcc
     1741 ttaagcccgc gttaagccgt tagcaacgac gctcggggca tgtgcgcgcc tcaaggcgcc
     1801 gcgcggcgcg tggtcgccgc cgtcaccgac gatctgcagc cgcggattcg cgccattttt
     1861 cccggctgcg acgtgcgctt cgtgcgctcg ggaacagagc ttgtgcgggc gctcgacgaa
     1921 gcgcgctgcg agatgatggt cgtacaggta cacttcaacg aatcgagcgc cgcggcggcg
     1981 ctcatgtgcg tgctcgcgcg cgaggagagt tttccggtgg tctgcgtgcg cgatgtgccc
     2041 gatgcgaagc cggcttacgc cgcgctcaat gcattgcgca tcgcgctcgg cggcatcgtc
     2101 gcgcgtgcct tcatcgacct cgtcgagcac cgcgacgatg aagcgggcaa tgcgcgcgtg
     2161 cgagcgatgc tcgagcctct cctttagcgc caggtcaatt ccacgcggcg atcgggctgc
     2221 aggcactcca ccagagcggc attgcggcgg ttctcgggtc ccaggtcaac acacgcgtcg
     2281 ccgctgacgg gttcgccctc gccca
//
LOCUS       DASUNT010000095         2738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_539650, whole genome shotgun sequence.
ACCESSION   DASUNT010000095 DASUNT010000000
VERSION     DASUNT010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539650"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /gene="ald"
                     /locus_tag="VFX94_01920"
     CDS             complement(<1..905)
                     /gene="ald"
                     /locus_tag="VFX94_01920"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX5766965.1"
                     /translation="MIIGVPREIKTHEYRVGLTPTSVRELVSHRHQVIVEKGAGAGIG
                     AADALYEKAGARIGDPWTAELIVKVKEPQREERARLRERQVLFTYLHLAPDREQTDDL
                     LRSGAVCIAYETVTSAHGGLPLLAPMSEVAGRMAVQVGASCLERKRGGMGILLGGVPG
                     VPPAKVTVLGAGVAGTNAIRIAVGTGARVYALDRSLEALRNIEREFGARVVTVHSSRD
                     TVESHVLSADLVIGAVLVPGAAAPRLVSRQMVKEMKQGAVVVDIAIDQGGCFETSRAT
                     THADPTYVVDGVVHYCVANMPGAVPR"
     gene            complement(902..2140)
                     /locus_tag="VFX94_01925"
     CDS             complement(902..2140)
                     /locus_tag="VFX94_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5766966.1"
                     /translation="MNFEYSPKVQELREKLARFMADHVYPSEASWRKHVTSEKRWEVV
                     PIIEELKPKARAAGLWNLWRPKTHGGTLTNLEYAPLCEIMGRVTWAPELFNCSAPDTG
                     NMETLLKYGSPEQIRQWCEPLLEGKIRSAFLMTEPNVASSDATNIQTRITRKNDDYVI
                     NGVKWWSSGAGDPRCKIYIVMGKTDPDNPSRHSQQSMVLVPADTPGVKILRHLPVFGY
                     DDAPHGHMEIELKDVRVPVSNVLLGEGRGFEIAQGRLGPGRIHHCMRLIGQAERALEK
                     MCRRASERTAFGRSIAEQGVTRERIAQARILIDAARYMVLNAAWKMDTVGNKVAKKEI
                     AMIKVFAPNVACQVIDWAMQVHGGGGVTEDFGLAEAYAHARTLRFADGPDEVHRNQIA
                     KIELGEQTSLPEALGKVRRM"
     gene            complement(2137..>2738)
                     /locus_tag="VFX94_01930"
     CDS             complement(2137..>2738)
                     /locus_tag="VFX94_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX5766967.1"
                     /translation="IGLEDFGKSGNYIERQIARWTAQYQASETERIEAMDNLIAWLPK
                     NIPPSAPTTVVHGDYRLDNAIFHAAEPRILAVLDWELSTLGDPLADFAYHCMSWHIPP
                     GQFRGIAGLDLEALGIPAEAEYVASYCERTRQKIDPSHWDFYLAYNLFRIAAILQGIM
                     KRVVDGTAASAHARDAGMRAKPMAQLGWQQAEKILRRAA"
BASE COUNT          404 a          952 c          856 g          526 t
ORIGIN      
        1 cgcggcacgg cgccgggcat gttcgccacg caatagtgca ccacaccgtc cactacgtac
       61 gtggggtcgg cgtgcgtagt cgcgcgcgag gtctcgaagc agccgccctg gtcgatcgcg
      121 atgtcgacca cgactgcacc ctgcttcatc tccttcacca tttgccggct gacgagcctg
      181 ggcgcggccg cgcccggaac gagcaccgct ccgatcacga ggtcggccga gaggacgtgg
      241 ctttcgacgg tgtcgcggct cgaatgcacc gtgaccacgc gcgcgccgaa ctctctctcg
      301 atattcctca acgcttccag cgagcggtcg agcgcgtaaa cccgggcccc ggtaccgacc
      361 gcgatgcgga tcgcgttcgt gccggcgacg cctgcgccga gcaccgtgac cttcgccggc
      421 ggcacgccgg gaacaccgcc cagcagaatt cccatgccgc cgcgtttcct ctccaggcag
      481 ctggcgccga cctggaccgc catgcggccg gcgacctccg acatcggcgc gagcagcggc
      541 agtccgccgt gcgcgctggt gaccgtttcg taggcgatgc agactgctcc gctacgcagc
      601 aaatcgtcgg tctgctcccg atcgggcgcg agatgcaggt aggtaaagag cacctgccgc
      661 tcgcgcagcc tggcacgctc ctcgcgttgc ggctccttca ccttgacgat gagctcggca
      721 gtccacggat cgcctatgcg cgcgccggct ttctcgtaga gcgcgtccgc ggcgccgatg
      781 ccggcccccg cacctttctc gacgatgact tggtgccgat gcgagacgag ctcgcgcacc
      841 gaggtcggcg tgaggcctac gcggtactcg tgcgtcttga tctccctcgg tactcctatg
      901 atcacatgcg cctcaccttg ccgagcgcct cgggcagcga ggtctgctcg ccgagctcga
      961 tcttcgcgat ctggttgcgg tgcacctcgt ccgggccgtc ggcgaaacga agcgtgcggg
     1021 cgtgcgcgta agcctccgcg aggccgaagt cctcggtcac gccgccgccg ccgtgcacct
     1081 gcatcgccca gtcgatcacc tggcaggcga cgttgggagc gaacaccttg atcatcgcga
     1141 tctccttctt cgccaccttg ttgcccacgg tgtccatctt ccatgccgcg ttcagcacca
     1201 tgtagcgcgc cgcgtcgatc aggatgcgcg cctgcgcgat gcgctcgcgc gtcacgccct
     1261 gctccgcaat gcttcttcca aaagccgttc tttcgctcgc ccgcctgcac attttctcga
     1321 gcgcgcgttc ggcctggccg atgagccgca tgcagtgatg gatgcgcccg ggtccgagcc
     1381 ggccctgggc aatctcgaac ccgcgccctt cgccgagcag cacgttggaa acgggtacgc
     1441 gcacgtcctt gagctcgatc tccatgtggc cgtgcggcgc atcgtcgtag ccgaacaccg
     1501 gcaggtggcg caggattttc acgcccggcg tgtccgccgg cacgagcacc atcgactgct
     1561 ggctgtggcg cgacgggttg tccggatcgg tcttgcccat cacgatgtag atcttgcagc
     1621 gcgggtcgcc cgcgccgctc gaccaccact tcacgccgtt gatcacgtag tcgtcgtttt
     1681 tccgcgtgat cctcgtctga atgttcgtcg catcggaaga ggcgacgttc ggctcggtca
     1741 tgaggaacgc cgagcggatc tttccttcga gcagcggctc gcaccattgc ctgatctgct
     1801 cggggctgcc gtacttgagc agcgtctcca tgttcccggt atcgggcgcc gagcaattga
     1861 agagctcggg cgcccaggtc acccggccca tgatctcgca gagcggggcg tactcgagat
     1921 tggtgagcgt cccgccgtgc gtcttcgggc gccacaggtt ccacagcccc gcggcgcgcg
     1981 ctttcggctt cagctcctcg atgatcggca ccacttccca gcgcttttcg ctcgttacat
     2041 gctttctcca gctcgcttcg ctcggataca catgatcggc catgaagcgg gcgagcttct
     2101 cgcgcagctc ctgcaccttg ggcgaatatt cgaagttcac gctgccctcc tcagtatttt
     2161 ttcagcctgc tgccagccga gctgtgccat cggcttcgcg cgcatgccgg cgtcgcgcgc
     2221 gtgcgcgctc gccgcggtgc cgtccactac gcgcttcatg atgccctgca ggatcgcggc
     2281 gatgcgaaac aggttgtagg cgaggtagaa atcccagtgc gacggatcga ttttctgccg
     2341 cgtgcgttcg cagtagctcg ccacgtactc ggcctccgcc ggaatgccga gcgcctcgag
     2401 atccaggccc gcgatgccgc gaaactggcc cgggggaatg tgccagctca tgcagtggta
     2461 cgcgaagtct gcgagcgggt caccgagcgt ggagagctcc cagtcgagca ccgccaggat
     2521 gcgcggctcg gcggcgtgga agatcgcgtt gtccaggcga tagtcgccgt gcaccacggt
     2581 ggtcggcgcg ctcggcggaa tattcttcgg cagccaggcg atcaggttgt ccatcgcctc
     2641 gatgcgctcg gtttccgagg cctggtattg cgcggtccag cgcgcgatct ggcgctcgat
     2701 gtagtttccg gactttccaa aatcctcgag accaattt
//
LOCUS       DASUNT010000096         2856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_977964, whole genome shotgun sequence.
ACCESSION   DASUNT010000096 DASUNT010000000
VERSION     DASUNT010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977964"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..443)
                     /locus_tag="VFX94_01935"
     CDS             complement(36..443)
                     /locus_tag="VFX94_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766968.1"
                     /translation="MSERLLRSAAFRVRAILLGVRLDLRNWPKAETLARAPLTTRLSG
                     GGIAVLFRYGVAVLLDATASAEQELRERLAPLTEHRYGSSEAEELDVRIDEARLEGLN
                     ALLEDEFEIRERLDIVGLIVFEILLSVYGMWRT"
     gene            524..724
                     /locus_tag="VFX94_01940"
     CDS             524..724
                     /locus_tag="VFX94_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766969.1"
                     /translation="MPWNESYYPASMKNLPPAVRAKAIQIANALLESGRPEGQAIRIG
                     IAGAKRWASALGQRPGRPVDRR"
     gene            729..1373
                     /locus_tag="VFX94_01945"
     CDS             729..1373
                     /locus_tag="VFX94_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX5766970.1"
                     /translation="MRVFDSRNDAGQRLARALYAYRGRDPLVLAIPRGAVAMAAVVAA
                     ELEGELDVVLVRKLRAPGSPEFAVGAVDETGWTYIAPYAAEVGADAVYLARERRAELE
                     VLRKRRGKYTPGRGSIDPAGRVVIVVDDGIATGATMIAALHAVRVRGPARLVCAVPVA
                     APDSLRQVRSYADETVCLETPESFHAVGQFYREFPQVDDEEVVALLAERRKMTA"
     gene            complement(1384..1890)
                     /locus_tag="VFX94_01950"
     CDS             complement(1384..1890)
                     /locus_tag="VFX94_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766971.1"
                     /translation="MNTPHVFVSYAPRGAGLRCATAYLAGERDVYGWYTGPGDDTRFT
                     AGFFLIEDFYTLRPAHYEAVEHGELHSRWALDEARRHELARMQEAFAREWLVYRDDPH
                     AAAELAAYAEAELAAGEVNLRFQRLAKLSTLQPNWTYYSPGFERAVLRFLAKRWPLEY
                     RLDLVEAA"
     gene            2054..2476
                     /locus_tag="VFX94_01955"
     CDS             2054..2476
                     /locus_tag="VFX94_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766972.1"
                     /translation="MMTKNEVQLDVEADRASAQWQQRADDLYWPLAVAAAVTFHQIHR
                     STKAFIIREDYDDALNLAAAALSRLIPIYTLRDPRRGREPVKVDLTRSQFARGATQLR
                     GAGETVEDLSVSRRDLLSALSLIKRTGLPFSFALRKEL"
     gene            complement(2641..>2856)
                     /locus_tag="VFX94_01960"
     CDS             complement(2641..>2856)
                     /locus_tag="VFX94_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766973.1"
                     /translation="IVLSALNLFHGADLPARAAVAMIALAYSSNVAFKLAILVWFNRR
                     VAWRALWPLAATLVAGGAVFVFSRNTL"
BASE COUNT          480 a          924 c         1019 g          433 t
ORIGIN      
        1 gtagaggtct gtgagcagcg ggctggaaag cgggttcatg tgcgccacat gccgtaaacg
       61 ctgagcagga tctcgaacac gatgaggccg acgatgtcca ggcgctcgcg gatctcgaac
      121 tcgtcctcga gcagcgcgtt caggccttcg aggcgagctt cgtcgatgcg cacgtcgagc
      181 tcttcggctt ccgagctgcc atagcggtgc tcggtgaggg gagcgagtcg ctcgcgcagc
      241 tcctgctcgg cgctcgccgt tgcgtcgaga agcaccgcca cgccgtagcg aaagagaacc
      301 gcaatgccgc ccccggagag ccgcgtcgtg agcggcgcgc gcgccagggt ctcggccttg
      361 ggccagttgc gcaggtcgag ccgcacgccg aggagaatcg cgcgcacccg gaacgccgcg
      421 gagcgcagca gcctttcgga catcgctcga ttaagccagc cggccatgtc aggtttttga
      481 tgcggatcaa ggcccgtccg gcctgccggt atgtattgat ggcatgccgt ggaacgaaag
      541 ctactacccg gcatcgatga agaacctgcc tcccgcggtg cgcgcgaagg cgatccagat
      601 cgcgaatgcg ctcctcgaga gcgggcgccc ggagggccag gcgatccgca tcggcatcgc
      661 gggcgcgaag cgctgggcgt ctgcgctagg tcaaaggccc ggacgtcccg ttgaccgaag
      721 atagcgctat gcgggttttc gattccagga acgacgccgg ccagcgcctc gcgcgtgcgc
      781 tttacgccta tcgcggtcgc gatccgctcg tgctcgcgat tccgcgcggc gcggtcgcga
      841 tggcggcagt cgtggccgcg gagctcgagg gcgagctcga cgtcgtgctg gtgaggaagc
      901 tgcgcgctcc cgggagcccc gagttcgcgg tgggcgcggt ggatgagacc gggtggactt
      961 acatcgcccc gtacgccgcg gaggtcggcg ccgatgcggt ttacctcgcg cgggaaaggc
     1021 gcgccgagct cgaagtgctg cgcaagcggc gcgggaaata cacgcccggg cgcggcagca
     1081 tcgatccggc ggggcgggtg gtgatcgtgg tggacgacgg catcgccacc ggcgcgacca
     1141 tgatcgccgc gctgcacgcg gtgcgcgtca ggggacccgc ccgcctcgta tgcgcggtgc
     1201 cggtggcggc gcccgacagc cttcggcaag tgcgctccta cgccgacgag accgtgtgcc
     1261 tggagacgcc agagtccttc catgcggtcg ggcagttcta ccgcgaattt ccgcaggtcg
     1321 acgacgaaga ggtcgtcgcg ctgctcgccg aacggcgcaa gatgacggcc tgatataccc
     1381 gggttacgcg gcttcgacga ggtcgaggcg gtactcgagc ggccagcgtt tggcgaggaa
     1441 gcgaagcacc gcgcgctcga agcccggcga gtagtacgtc cagttgggct gcagcgtgga
     1501 gagcttggcg agccgttgaa agcgcagatt gacttccccc gccgcgagct ccgcctcggc
     1561 gtaggcggcg agctccgccg cggcgtgcgg gtcgtcgcga tacacgagcc actcgcgcgc
     1621 gaaggcttcc tgcatccgcg cgagctcgtg gcggcgtgcc tcgtccagcg cccagcgcga
     1681 atgaagctcg ccgtgctcga ccgcctcgta gtgtgccggg cgcagggtgt agaaatcctc
     1741 gatgaggaag aagccggcgg tgaaccgcgt gtcgtcgccg ggcccggtat accagccgta
     1801 cacgtcgcgc tccccggcga ggtaggcggt cgcgcagcgc aaccccgcgc cgcgcggcgc
     1861 gtaggagacg aagacgtggg gagtgttcat tgcggctatt gcacctcagt ttcggcgcgg
     1921 tcgcttgatg acaatcaata gacaccgaga taagcattga ccggcaaaca cgagcgattg
     1981 cggcaacgac agattcccct tgcagaacac cgcgacacgg cggaaactgg ccgccagccg
     2041 cggaggggtg cgcatgatga cgaagaacga ggtacagctc gacgtcgagg cggaccgcgc
     2101 gagcgcgcaa tggcagcaac gcgccgatga tctgtactgg ccgcttgccg tggcggccgc
     2161 cgtgacgttc caccagatcc accgcagcac aaaggcgttc atcatccgcg aggactacga
     2221 cgatgcgctg aacctcgccg cggcggcgct ttcgcgcctc atcccgatct acacgctgcg
     2281 tgatccgcgc aggggccgcg agccggtgaa ggtcgatctc acccggtcgc agttcgcgcg
     2341 cggcgcgacc cagctgcgcg gcgccggcga gaccgtcgag gacctgtcgg tgtcgcggcg
     2401 cgatcttctc tcggcgctct cgctcatcaa gcgcaccggc ctgccgttca gtttcgcgtt
     2461 gcgcaaggag ctctgagcgc gaatcgaaac ttctttttgt atcgacgaga gcgcctagcg
     2521 cgccaggctg tcgatgccga ggtcgcgctc gagctgcgcg tcgggatcga cccgcgccgc
     2581 cgcaccgctc ggcaagcatc gcgccgatga tctcggtgag ccggctcatt gcggggcgcg
     2641 ctataaagtg tttctggaga acacgaagac cgcgccgccg gcgacgagcg tcgcggcaag
     2701 cggccagagc gcgcgccagg cgaccctgcg gttgaaccag acgaggatcg cgagcttgaa
     2761 ggccacgttg gatgaatagg cgagcgcgat catcgccacc gcggcgcgcg ccggcaggtc
     2821 cgcgccgtgg aagaggttga gggccgagag cacgat
//
LOCUS       DASUNT010000097         2278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_890634, whole genome shotgun sequence.
ACCESSION   DASUNT010000097 DASUNT010000000
VERSION     DASUNT010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890634"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFX94_01965"
     CDS             <1..290
                     /locus_tag="VFX94_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEX5766974.1"
                     /translation="CIARALATGPELLLFDEPTSALDPIATASIEELMHDLKERVTIL
                     IVTHNMQQAARVSDFTAYMYLGDLIEFGVTDELFIKPKMKQTEDYITGRFG"
     gene            296..988
                     /gene="phoU"
                     /locus_tag="VFX94_01970"
     CDS             296..988
                     /gene="phoU"
                     /locus_tag="VFX94_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX5766975.1"
                     /translation="MTEHASKQYDLDLGSIRSRVLAMGGLVESQIRRAIDALTTGNVA
                     LSEEVIAADHKVNALEVELDGDCSQLIVRRQPAASDLRMIFAIMKTVTDLERIGDEAQ
                     KIARMAKNIHERGVSAPPVVEVRHAAEAGIAMLRRTLDCFARLDVDAAAEVIREDAGI
                     DTEFRSILRQLITYMMEDPRTISTALEIVWIAKAIERIGDHAKNMAEYVIYIVKGTDV
                     RHTGSARLEVKE"
     gene            985..1671
                     /gene="phoB"
                     /locus_tag="VFX94_01975"
     CDS             985..1671
                     /gene="phoB"
                     /locus_tag="VFX94_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEX5766976.1"
                     /translation="MSATVLVVEDEPQIQELVAVNLEHIGHKVLRAASAEEAEAAIRA
                     ALPDVLVLDWMLPGESGLAFARRLRADERTRELPILMLTARAMEQDKLSGLEAGADDY
                     LTKPFSPKELAARIKAVLRRRAPQLSGDAVEVEGLRLDPATRRVTAHGKRVDLSPSEF
                     RLLHFLMTHPERVYSRAQLLDHVWGDHVFIEERTVDVHIRRLRKALESSGHDRLIDTV
                     RGSGYGLRTD"
     gene            1672..>2278
                     /locus_tag="VFX94_01980"
     CDS             1672..>2278
                     /locus_tag="VFX94_01980"
                     /inference="COORDINATES: protein motif:HMM:NF023236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor protein PhoR"
                     /protein_id="HEX5766977.1"
                     /translation="MVYRNTVLWTAGFAAAGFLAGLLISPAWGWSVLAAGLLALLFWH
                     ARHLSRLRRWLANPLPGQVPEGEGIWDEVLAALHRLERDAAKRERRLADALARMRGAV
                     QALPDGVVILDSANRIEWCNRTAERQLMLDARADLGHNIANLVREPEFVDYLAQPQEK
                     AVRVTRGQGMALSLQLILVGETQKLLLARDVTQAERVDAMRR"
BASE COUNT          386 a          784 c          766 g          342 t
ORIGIN      
        1 tgtgcatcgc gcgcgcgctc gccaccggtc cggagctcct gctcttcgac gagccgactt
       61 cggcgctcga cccgatcgcc accgcctcga tcgaggagct gatgcacgac ctgaaggaac
      121 gcgtcaccat cctgatcgtg acgcacaata tgcagcaggc ggcgcgcgtg tccgacttca
      181 ccgcgtacat gtacctcgga gacctgatcg agttcggcgt caccgacgag ctcttcatca
      241 agccgaagat gaagcagacc gaggactaca tcaccggcag attcggctga tgggcatgac
      301 cgagcacgcc tccaaacaat acgacctcga cctcggctcg attcgctcgc gcgtgcttgc
      361 catgggcggc ctggtggaat cgcagatccg gcgcgcgatc gatgcgctca cgaccggcaa
      421 cgtcgcgctt tccgaggaag tcatcgcggc agaccacaag gtgaacgcgc tggaggtcga
      481 gctcgacggc gactgcagcc agctcatcgt gcgccgccag cccgcggcga gcgacctgcg
      541 catgatcttc gccatcatga agacggtgac cgacctcgaa agaatcggcg acgaggcgca
      601 gaagatcgcg cgcatggcca aaaacatcca cgagcgcggc gtatcggcgc cgccggtggt
      661 ggaggtgcgc cacgccgccg aggcggggat cgcgatgctg cggcgcacgc tcgactgctt
      721 cgcgcgcctc gacgtcgacg ccgccgccga ggtcatccgc gaggacgccg gcatcgacac
      781 ggaatttcgc tcgatcctgc gccagctcat cacgtacatg atggaagatc cgcgcaccat
      841 ctcgaccgcg ctcgagatcg tgtggatcgc caaggcgatc gagcgcatcg gcgaccacgc
      901 caagaacatg gccgagtacg tgatctacat cgtcaagggc accgacgtgc ggcacaccgg
      961 aagtgccagg ctcgaggtca aggaatgagc gccactgtcc tcgtggtcga ggacgagccg
     1021 cagatccagg agctcgtcgc ggtgaacctg gagcacatcg gccacaaggt gctgcgcgcc
     1081 gcgagcgccg aggaggccga ggccgcgatc cgcgccgcgc tgcccgacgt gctggtgctc
     1141 gactggatgc tgcccggaga atccgggctt gccttcgcgc ggcgcctgcg cgccgacgag
     1201 cgcacgcgcg agctgccgat cctgatgctc accgcgcgcg ccatggagca ggacaagctc
     1261 tcgggcctgg aggcgggcgc cgatgattac ctcaccaagc ccttctcgcc caaggagctc
     1321 gctgcgcgca tcaaggcagt gctccggcgc cgggcgccgc agctttccgg cgacgccgtc
     1381 gaggtggaag ggctccggct cgacccggcc acccggcgcg tgaccgcgca cggcaagcgc
     1441 gtggaccttt caccgtcgga gtttcgcctg ctccacttcc tgatgaccca tccagagcgc
     1501 gtctacagcc gcgcgcagct cctcgatcac gtctggggcg atcatgtgtt catcgaggag
     1561 cgcaccgtgg acgttcacat ccgccgcttg cgcaaggcgt tggagtcgag cgggcacgac
     1621 cggctgatcg acaccgtgcg cggctcggga tacggcttgc gcaccgattg agtggtttac
     1681 cggaacaccg tgctgtggac cgcgggcttc gcggcggccg gttttctcgc cggcctgctc
     1741 atcagcccgg cgtggggatg gagcgtgctt gccgccggcc tccttgcgct gcttttctgg
     1801 cacgcgcggc acctctcgcg cctgcggcgc tggctggcaa acccgctgcc gggccaggtg
     1861 ccggaaggcg agggcatctg ggacgaagtg ctcgccgcgc tgcatcgcct ggagcgcgac
     1921 gcggcgaagc gcgaacgtag gcttgccgat gcgctcgccc gcatgcgtgg cgcggtacag
     1981 gcgctgcccg acggcgtggt gattctcgac agcgcgaacc gcatcgaatg gtgcaaccgg
     2041 accgccgagc ggcagctgat gctcgacgcg cgtgccgacc tcggccataa catcgccaac
     2101 ctcgtgcgcg aaccggagtt cgtcgactac ctggcgcagc cccaggaaaa ggcggtgcgc
     2161 gtgacgcgcg gccagggcat ggccctgtcc ctgcagctga tcctcgtcgg cgagacgcag
     2221 aagctgctcc tcgcgcgcga cgtcacgcag gccgagcggg tggacgccat gcggcgcg
//
LOCUS       DASUNT010000098         4251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_102208, whole genome shotgun sequence.
ACCESSION   DASUNT010000098 DASUNT010000000
VERSION     DASUNT010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102208"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2143
                     /locus_tag="VFX94_01985"
     CDS             <1..2143
                     /locus_tag="VFX94_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5766978.1"
                     /translation="AADGREALAKAREARPELVISDVLMPLMDGYELARRLKADPATA
                     SAALMFYTAYFGGQDALALAHAHGVSRVLLKPSDNATILRQVETVLASRRETPAQIAP
                     DLDQGHLRVVVDQLLEKTGALEAQQRRIERLNRTLAVLSAVNALIVRAGERQALLEEA
                     CRIAVEKGGFRLAAIGLVDAASQAIRQVAATGEGSAEREFARLRLGTRSLIWNDNSGS
                     FVALPLLAAGESAGLLLLYANQRDFFDEEEMRLLHELAGDIAFALRHLAQKARMDYLA
                     YHDSLTDLPNRSLFTDRMTQALSAARREKRFAAAIFVDVERFRMLNASFGRKRGDDLL
                     REVGARLRRAAREQDTVARVGADHFAIAVAPFDRPKDTAYAFLERLDEALAQPIAIDG
                     AELRVAVKAGIAVFPTDGDSTEALYANAETALLKAKRQGSRYLFYAPEMNARVAESLA
                     MENRLRRALDDGTLALHYQPKIDVATGEVAGLEALIRWNDAELGAVPPARFVSLLEET
                     GMILAAGRWALHKAVDDIRRWQSLGLKVPRTSVNVSAVQLRQKDFVESVLKVIAEIGG
                     KSPLLDLEITESVLVDDIDESTRKLQALRRAGVEVSVDDFGTGFCSLSYLARLPVDTL
                     KIDRSFVVRMPGAGYPRTIVAMIVSLAHTLGLRVVAEGVEDTEQVRLLRELGCDQIQG
                     FYVSRPVPREDVDAMLGPGASKSLQEKLAAA"
     gene            2202..3047
                     /locus_tag="VFX94_01990"
     CDS             2202..3047
                     /locus_tag="VFX94_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766979.1"
                     /translation="MASVVDQTPYQDRRIGERRRLARAIPMRAAEGMRVSWGGIFGGV
                     LCAVGLLLLLAALGVAIGITAADPAETRASTIGTGAGIWAGVSLLLALFLGGLVATRI
                     GAISDRTTGFFEGALVWVVTILLMGYLATSGISTVAGGIFNMLGGATKAVGSVMQAGS
                     MDVDLSGSVDQMVQRLRDPRTAQQIAAATGMARFEVEKALSETAQRVQQSRDNPAQAA
                     AEAKQRLAQLVDQAKSSGALQQKAAELQPQASRAAWMTLGALLLSLLAAVIGAMAGRR
                     RVPMV"
     gene            complement(3112..>4251)
                     /locus_tag="VFX94_01995"
     CDS             complement(3112..>4251)
                     /locus_tag="VFX94_01995"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEX5766980.1"
                     /translation="TDAYQPGMELHGKYTFFAEGCRGHLGRQLEAKLGLRAGVDPQVY
                     GIGLKELWELKPERHQPGLVVHTAGWPLDPGTYGGSFMYHMEDRLVAVGFVVGLGYAN
                     PYLSPYEEFQRFKTHPEIRKTFENAKRICYGARAIASGGLQSLPKLVFPGGALIGDDA
                     GFLNAARIKGSHCAIKSGMLAAEAAHEALEGGRARDELTVYPQAFRSSWLYEELHRAR
                     NFKPWMAKGLYVGTLMFGIDQTVFRGKAPWTLSHHVADHETLKPKAEATPIAYPKPDG
                     ALAFDRLSSVFISNTNHSEDQPVHLTLKDPSIPVAVNLETYAGPEQRYCPAGVYEFVD
                     VDTNPRLQINAQNCVHCKTCDIKDPKQNIVWVAPEGGGGPNYVNM"
BASE COUNT          668 a         1411 c         1472 g          700 t
ORIGIN      
        1 cgcggccgac gggcgcgagg cgctcgcgaa agcccgcgag gcgcggcccg agctcgtgat
       61 ctccgatgtg ctgatgcccc tcatggacgg ctacgagctc gcgcggcggc tgaaggcgga
      121 tccggccacc gcgagcgccg cgctcatgtt ctacaccgcg tacttcggcg ggcaggatgc
      181 gctcgcgctc gcgcacgcac acggcgtctc ccgcgtgctg ctcaagccct cggacaacgc
      241 cacgatactg cgccaggtcg agaccgtgct cgcctcgcgc agggagaccc cggcgcagat
      301 cgcccccgac ctcgaccagg gccacctgcg cgtggtcgtc gatcagcttc tcgagaagac
      361 cggggcgctg gaagcgcagc agcggcgcat cgagcggctg aaccgcacgc tggcagtcct
      421 gagcgctgtg aacgcgctca tcgtgcgcgc cggcgagcgg caggcgctgc tcgaggaggc
      481 gtgccgcatc gccgtggaga agggcggatt tcgtcttgcg gcgatcgggc tcgtcgatgc
      541 tgcgagccag gcgatccgcc aggtcgcggc cacgggcgag ggcagcgcgg agcgcgagtt
      601 cgcgcgcctc aggctcggca cgcgctctct catctggaac gacaacagcg gctcgttcgt
      661 ggcgttgccg ctgctggcgg cgggcgagag cgcgggcctt cttctgctct acgcgaacca
      721 gcgcgacttc ttcgacgagg aggagatgcg cctgctgcac gagctcgccg gcgacatcgc
      781 cttcgcgctg cgtcacctcg cgcagaaggc gcgcatggat tacctcgcct atcacgactc
      841 gctcaccgat ctgcccaacc gcagcctctt caccgatcgc atgacgcagg cgctgagcgc
      901 ggcgcggcgc gagaagcgct tcgcggcggc gatcttcgtc gacgtggaac gctttcgcat
      961 gttgaacgcg agcttcggtc gcaagagggg cgatgacctg ctgcgcgagg tgggcgcgcg
     1021 cctgcgccgc gcggcgcgcg agcaggacac ggtggcgcgg gtgggcgccg accactttgc
     1081 gatcgcggtt gcgccgttcg accggccgaa ggacaccgcg tatgccttcc tcgagcgcct
     1141 cgacgaggcg ctcgcgcagc cgatcgccat cgatggcgct gagctgcgcg tcgcggtcaa
     1201 ggccgggatc gccgttttcc cgaccgacgg ggactcgacc gaggcgctgt acgcaaatgc
     1261 cgagaccgcg ctgctcaagg cgaaacggca gggcagccgg tatcttttct acgcgccaga
     1321 gatgaacgcg cgcgtcgcgg agtcgctcgc gatggagaac cggctgcgcc gcgcgctcga
     1381 cgacggtacg ctcgcgctgc actaccagcc caagatcgac gtcgcgaccg gcgaagtcgc
     1441 gggcctggag gcgctcatcc gctggaatga cgccgaactg ggcgcggtgc cgcccgcgcg
     1501 cttcgtctcg ctgctcgagg aaaccgggat gatcctcgcc gcgggccgct gggcgctgca
     1561 caaggcggtc gatgacatcc ggcgctggca gtcgctcggg ctcaaggtgc cgcgcacttc
     1621 ggtcaatgtg tccgccgtgc agctcaggca aaaggacttc gtcgagtcgg tgctgaaagt
     1681 gatcgccgag atcggcggca agtcgccgct tctcgacctc gagatcaccg aaagcgtgct
     1741 ggtggacgac atcgatgaga gcacgcgcaa gctccaggcg ctgcgccggg cgggcgtgga
     1801 ggtctcggtg gacgatttcg gcaccgggtt ctgctccctg agctacctcg cgcgcctgcc
     1861 ggtggatacc ctgaagatcg atcgcagctt cgtcgtgcgc atgcccggcg ccggctaccc
     1921 tcgcaccatc gtcgccatga tcgtgtcgct cgcgcacacg ctcgggctgc gcgtggtcgc
     1981 cgagggcgtc gaggacaccg agcaggtgcg cctgctgcgc gagctcggct gcgaccagat
     2041 ccagggcttc tacgtgagcc gccctgtccc tcgcgaggac gtcgatgcca tgctcggccc
     2101 cggcgccagc aagtcgctgc aggagaagct cgccgcggca tagcccccac agccggcgcg
     2161 aaacttgcgg gggcgtcgcg atcgcagaga aaggacaggc tatggcatcc gtagttgacc
     2221 aaacccctta tcaagacagg cgtattgggg agcgccggcg cctggcgcgc gcgatcccca
     2281 tgcgcgcggc cgaaggcatg cgcgtgtcct ggggcggcat tttcggcggc gtgctgtgcg
     2341 ccgtcggcct tctgcttcta cttgccgcgc tcggcgtggc gatcgggatc acggccgcag
     2401 acccggccga gacccgggct tcgactatcg gtaccggcgc cggcatctgg gccggcgtgt
     2461 cgctgcttct cgcgctcttc ctcggcgggc tggtcgcgac gcgcattggc gcgatctcgg
     2521 accgcaccac gggatttttc gagggcgcgc tcgtctgggt cgtcacgatc ctccttatgg
     2581 gttacctcgc cacgagcggg atcagcaccg tggcgggcgg aatcttcaac atgctgggcg
     2641 gagcgaccaa agccgtcgga tcggtcatgc aggcgggcag catggatgtt gatctttccg
     2701 gcagcgtgga ccagatggtg caacgcctca gggacccgag gacggcgcag cagattgcgg
     2761 ccgcgaccgg catggcgcgc ttcgaggtgg agaaggcgtt gagcgagacc gcccagcgcg
     2821 tgcagcagag ccgcgacaat cccgctcagg cagccgccga ggcaaagcag cgcctggcgc
     2881 agctcgtcga tcaggcgaaa tccagcggag cgctccagca aaaagccgcg gagcttcagc
     2941 cgcaggcatc gcgcgcggca tggatgacgc tcggcgcgct gctgctttcg cttctcgccg
     3001 cggtgatcgg cgccatggca ggtcggcggc gcgtgccgat ggtctgacgg ggacgaagcc
     3061 ctaagttacc ggtaaagcta cgaagttagg gctccgtccg ccgaattggc cctacatgtt
     3121 gacgtagttc gggccgccac cgccttcggg agcgacccag acgatgttct gtttgggatc
     3181 cttgatgtcg caggtcttgc agtgcacgca gttctgcgcg ttgatctgca ggcgcgggtt
     3241 cgtgtcgaca tcgacgaact cgtagacgcc cgcggggcag tagcgctgct ccggccctgc
     3301 gtacgtttcg agattcaccg cgaccgggat gctcgggtcc ttcagcgtga gatgaaccgg
     3361 ctggtcttcg ctgtggttcg tgttcgaaat gaacaccgag gacagccgat cgaaggctag
     3421 cgctccgtcc ggcttcggat acgcgatggg ggtcgcctca gccttgggtt tcagcgtttc
     3481 gtggtccgcg acgtggtggc tcaaggtcca gggcgccttg ccgcgaaaca ccgtttggtc
     3541 gatgccgaac atcagcgtgc cgacgtagag tcccttcgcc atccagggct tgaagttgcg
     3601 cgcgcggtga agctcctcgt agagccagct cgagcggaac gcctgcggat aaacggtgag
     3661 ctcgtcgcgg gcgcggccgc cctcaagcgc ttcgtgcgcc gcctcggcag cgagcatgcc
     3721 ggacttgatc gcgcagtggc tgcccttgat gcgcgcagcg ttcaggaagc cggcgtcgtc
     3781 gccgatcagc gcgccgcccg ggaacacgag cttcggcagc gactgcaggc cgccggaggc
     3841 gattgcgcgt gccccgtagc aaatacgctt ggcattttca aacgtctttc ttatttccgg
     3901 gtgcgtcttg aagcgctgga attcctcgta gggcgagaga tacgggttgg catagcccag
     3961 gcccacgacg aagcccaccg cgaccaggcg atcctccatg tgatacatga acgagccgcc
     4021 gtacgtgccc gggtcgagcg gccagccagc ggtatggacc acgagtccgg gctggtgacg
     4081 ctccggcttc agctcccaga gctccttcaa cccgatgccg tagacctgcg ggtcgacgcc
     4141 cgcgcgcagg ccaagcttcg cctcgagctg ccgaccaaga tggccacggc atccctcggc
     4201 gaagaaggtg tacttcccat gcaactccat tcccggctgg taggcatccg t
//
LOCUS       DASUNT010000099         2323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1154716, whole genome shotgun sequence.
ACCESSION   DASUNT010000099 DASUNT010000000
VERSION     DASUNT010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154716"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="VFX94_02000"
     CDS             <1..857
                     /locus_tag="VFX94_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5766981.1"
                     /translation="FISADGRLKPEFARRGIQLERSVDPEIIYLYFNMQDKTVGGLSK
                     EKIALRRAIAMAYRIEDQIRIIRKGQSIRAHYPIPPGVAGHDPEYRSSIEHDARAANA
                     LLDKFNYRRGADGYRRMPNGEPLVIRYSSTPSERDRQFDELMKRSLDSISVRLEIHKD
                     RFPELIQLENQCRLMMRGAAWIADYPDGDNFMQLLYGPNSGQSNNACYRSPEYDKRYE
                     KSRLLPHGPERDKLYREMTRIMETDTVWLLTDSRYRNVLLQPHVKGFKKHPVLHAEWL
                     YIDLALPR"
     gene            838..1776
                     /locus_tag="VFX94_02005"
     CDS             838..1776
                     /locus_tag="VFX94_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5766982.1"
                     /translation="MLRYLARRLAQFIPTLLGVVLLVFLVFNWVGGDPAYILAGKIAN
                     PERIEEIRRGLGVDQPYWVQLWIFVKQVAVGDFGASWSTNERVSQIFATRLGPSLTVL
                     IPLLLVSTILAMALAMVVAYYRGSLTDRAVMIACTVGQSISILVYILVFQYLFAYKLG
                     WFPVQGWGGTLAENLFAYAALPILVGVVVSLAPDIRLYRSFFLEEINQDYVRTARAKG
                     LGEGRVMWLHVLRNAAIPIITNVMTMIPGLLAGAFLIERFFSIPGIGREVILAVERSD
                     FPVIKAVTIYVAIATMVVNLAADLVYRALDPRVQLR"
     gene            1773..>2323
                     /locus_tag="VFX94_02010"
     CDS             1773..>2323
                     /locus_tag="VFX94_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5766983.1"
                     /translation="MNLWLLAGQRLARDRVGLVSGLIVLAFVAIAAASAAGLVARDWS
                     AEVGVSYAPPVFLKSENREEGATAVGTYRAREEHGIADPLAEELAEIRRNLTGASATE
                     AREQTLLLGGDKWGRDVVKKAIKGTETSLVVGLAAATLAAFIGTFFGALAGYFGGWLD
                     DFFNWLYSVFTSIPYLLLVLAIAA"
BASE COUNT          385 a          796 c          716 g          426 t
ORIGIN      
        1 ccttcatcag cgccgacggc cggctcaagc ccgagttcgc caggcgcggc atccagctcg
       61 agcgcagcgt cgaccccgag atcatctacc tgtacttcaa catgcaggac aagacggtcg
      121 gcggcctgtc gaaggagaag atcgcgctgc gacgcgcgat tgcgatggcc taccgcatcg
      181 aggaccagat ccgcatcatc cgcaagggtc agtcgatccg cgcgcattac ccgattccgc
      241 cgggcgtcgc cggccacgat ccggaatacc gctcgagcat cgagcacgat gcgcgcgcgg
      301 cgaacgcgct tctcgacaag ttcaattacc ggcgcggggc ggacgggtac cggcgtatgc
      361 cgaacggcga gccgctcgtc atccgctact cctcgacgcc gagcgagcgt gaccggcaat
      421 tcgacgagct catgaagcgc tcgctcgact cgatcagcgt gcgcctggag attcacaagg
      481 atcgcttccc cgagttgatc cagctcgaaa accagtgccg cctcatgatg cgcggcgccg
      541 cctggatcgc cgattacccc gacggcgaca acttcatgca gctcctttac gggccgaaca
      601 gcggccagag caacaacgcc tgctaccgct cgccggaata cgacaagcgc tacgagaaat
      661 cgcgcctgct gccgcacggc ccggagcgcg acaagctcta tcgcgagatg acgcgcatca
      721 tggagaccga cacggtctgg ctcctcaccg acagccgcta ccgcaacgtg ctgctgcagc
      781 cgcatgtgaa ggggttcaag aagcacccgg tactgcacgc cgagtggctg tacatcgatc
      841 ttgcgctacc tcgctaggcg gctcgcgcaa ttcattccca cgctcctcgg cgtggtgctc
      901 ctcgtcttcc tggtcttcaa ctgggtgggc ggcgatccgg cctacattct ggccggcaag
      961 atagcgaacc ccgagcgcat cgaggaaatc cgccgcggcc tcggcgtcga ccagccgtac
     1021 tgggtgcagc tctggatctt cgtcaagcag gtggcggtgg gcgacttcgg cgcgagctgg
     1081 tccacgaacg agcgcgtgtc gcaaatcttc gccacgcgcc tcggtccttc gctcaccgtg
     1141 ctcatcccgc tcctcttggt cagcacgatc ctagccatgg cgctcgcgat ggtggtcgcg
     1201 tactaccgtg gctcgctcac cgaccgggcc gtcatgatcg cctgcaccgt cggccaatcg
     1261 atcagcatcc tcgtctacat cctcgttttc caatacctct ttgcgtacaa gctcggctgg
     1321 ttcccggtgc agggatgggg cggcacgctc gccgagaacc ttttcgccta tgcggcgctg
     1381 ccgatcctcg tcggcgtggt ggtctcgctc gcgcccgata tccgcctcta tcggagcttc
     1441 ttcctcgagg agatcaacca ggattacgta cgcacggcgc gcgccaaggg cctcggcgag
     1501 ggacgcgtga tgtggctgca tgtgctgcgc aacgccgcga ttccgatcat taccaacgtc
     1561 atgaccatga tcccgggcct gctggccggg gccttcctca tcgagcgctt cttctcgatc
     1621 cccggcattg gccgtgaggt cattctggcg gtagagcgca gcgacttccc ggtgatcaag
     1681 gcggtcacga tctacgttgc catcgccacc atggtcgtca acctcgccgc cgacctcgtc
     1741 taccgcgcgc tcgacccgcg cgtgcagctt cgatgaacct gtggctcctt gccgggcagc
     1801 gcctggcgcg cgatcgcgtc ggcctggtgt ccggcttgat cgtgctcgcg ttcgtcgcca
     1861 tcgcagcagc ctcggccgcg ggcctggtcg ctcgcgactg gtcggcggag gtcggcgtga
     1921 gctacgcccc gcccgtattt ctcaaatcgg aaaaccggga agagggcgct accgcagtag
     1981 ggacgtaccg tgcgcgcgag gagcacggca tcgccgatcc gcttgcggag gagctggcgg
     2041 agatccgcag gaacctgacg ggcgcttccg caaccgaggc gcgcgagcag acgctgctgc
     2101 tcggcggcga caagtgggga cgcgacgtgg tgaagaaggc gatcaagggg acggaaactt
     2161 cgctcgtggt cggccttgcc gccgctacgc tcgccgcgtt catcgggacg ttcttcggtg
     2221 cactcgccgg gtacttcggc ggctggctcg acgacttctt caattggctc tacagcgtgt
     2281 tcacctcgat tccgtatctc ctgctcgtcc ttgccatcgc cgc
//
LOCUS       DASUNT010000100         3087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_14544, whole genome shotgun sequence.
ACCESSION   DASUNT010000100 DASUNT010000000
VERSION     DASUNT010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14544"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFX94_02015"
     CDS             complement(<1..200)
                     /locus_tag="VFX94_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766984.1"
                     /translation="MKLPVLLAACFLASSTAFAQAPQGDASKAPRKARFDCSQAKDPK
                     ACEERRAKVKAAHDKAAKACEG"
     gene            complement(281..790)
                     /locus_tag="VFX94_02020"
     CDS             complement(281..790)
                     /locus_tag="VFX94_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5766985.1"
                     /translation="MAIKVGERLPDGKLMESTEFDSATQCAMAPREVNVAEAVKGKKI
                     AIFGLPGAYTPTCSAKHVPSYVQNLDQLSKKGVNEIWCVATNDAFVMAHWGRDQKALG
                     KIRFLADGSGQWTRALGLELDLNARGMGTRMNRFSMLVDNGVVKQLNVEGPGKFEVSD
                     AGTMLKQLG"
     gene            850..1056
                     /locus_tag="VFX94_02025"
     CDS             850..1056
                     /locus_tag="VFX94_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766986.1"
                     /translation="MRHLGTALIVIAGAIGALGIAVYATERGAANEIKGFVLVLIAAV
                     LLVGGCLLRELNLLRRSLEQKTLK"
     gene            1083..1505
                     /locus_tag="VFX94_02030"
     CDS             1083..1505
                     /locus_tag="VFX94_02030"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEX5766987.1"
                     /translation="MQRLAWLLDASIPLPGTRWSIGLDALIGFVPFLGDLVGVALSTY
                     IVAEAARLGASRGVLGRMVLNIALEGLAGLVPLAGDVFDAAWKANQRNVRLLGAWLDR
                     PRRAERSSALFLTVLALALLGIAAVPVVLTVVVIRWLL"
     gene            1515..2192
                     /locus_tag="VFX94_02035"
     CDS             1515..2192
                     /locus_tag="VFX94_02035"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEX5766988.1"
                     /translation="MWHKRIAVGLALLAAALLVLSGVGVRAGLWPFRVGFGMFAGALV
                     AGLAATGTALVALLVPRLRAGAVSALLFALILGAASAALPLENLRRVKTLPYINDITT
                     DTQAPPQFSQPRPYERHFAELQKLGYPDLVPLELAVPPPQAFARAREAAHGLGWEIVA
                     ADEAAGRMEAVATTLWFGFKDDVTVRIVPTAKGSRIDVRSRSRVGRSDIGANADRIRK
                     FFSVLKG"
     gene            2257..2502
                     /locus_tag="VFX94_02040"
     CDS             2257..2502
                     /locus_tag="VFX94_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HEX5766989.1"
                     /translation="MKHLIFLVITGLAAHAWAQAPAKEAAKPEPAKEAAKPEAAEPQP
                     AKHAVVIPKKRRWHEDARHCLERPTNTDIIKCAEEYL"
     gene            complement(2625..2882)
                     /locus_tag="VFX94_02045"
     CDS             complement(2625..2882)
                     /locus_tag="VFX94_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766990.1"
                     /translation="MRGVTLLLLVCLASCSPAPDAELRLVTIHDGEAGFSLRELPRAT
                     LRSLGLPYGLTVVRTGGAAERAGLRLGDVVYGLGAQARAKP"
BASE COUNT          473 a         1041 c         1012 g          561 t
ORIGIN      
        1 ttgccttcgc aggccttggc agccttgtca tgcgctgcct tcaccttcgc acggcgctcc
       61 tcgcaggcct tggggtcctt cgcctgcgag cagtcgaagc gcgccttgcg cggggctttc
      121 gacgcgtcgc cctgcggcgc ctgggcgaac gcggtggagg aggcgagaaa acacgcggcc
      181 agcagaacgg ggagtttcat gacggtcctt tgtggggtcg tgaccatccc ttaacgctcc
      241 ccggcgccgc cggccgacag ccagttgtaa cgaactgtgt ctatcccagc tgcttcagca
      301 tggtgccggc gtcggacacc tcgaacttgc cggggccctc gacgttgagc tgcttgacca
      361 cgccgttgtc gaccagcatc gagaagcggt tcatccgcgt gcccatgccg cgcgcgttga
      421 ggtcgagctc caggccgagc gccctggtcc actggccgct gccgtcggcg aggaagcgga
      481 tcttgccgag cgctttctgg tcgcggcccc agtgcgccat gacgaacgcg tcgttggtcg
      541 ccacgcacca gatctcgttc acgccctttt tgctgagctg atcgagattc tgcacgtagc
      601 tcggcacgtg cttcgccgag caggtcggag tgtaggcgcc gggcaggccg aagatcgcga
      661 tcttcttgcc cttgaccgct tcggccacat tcacttccct gggtgccatc gcgcattgcg
      721 tggcggagtc gaattcggtg gattccatca gcttgccgtc gggcaggcgc tcgccgactt
      781 tgatcgccat ggcgcatctc ctcgtggtcg tcttgggggg aggcgaaatg atacctttag
      841 gaaaggcgca tgcgacatct cggcacagca ctcatcgtca tcgcgggggc gatcggcgcc
      901 ctcggtatcg ccgtgtacgc taccgagcgc ggcgcggcta acgagatcaa ggggttcgtg
      961 ctcgtcctga tcgctgccgt gctcctcgtg ggcggctgcc tgctgcgcga gctgaatctc
     1021 ctgcgccgca gcctcgagca gaagacgctc aaatagacaa gagaagagaa gtcgagcgcc
     1081 ggttgcagcg gctggcgtgg ctgctcgacg cttccatccc gctcccgggc acgcgctggt
     1141 cgatcgggct cgacgcgctg atcggcttcg tgccgttcct cggcgacctg gtcggtgtgg
     1201 cgctgtcgac ctacatcgtg gccgaagcgg cacggctcgg cgcgagccgc ggcgtcctcg
     1261 gtcgcatggt gctcaacatc gcgctcgagg ggctggcggg cctcgtgccg ctcgcgggcg
     1321 atgtattcga tgccgcgtgg aaagcgaacc agcgcaacgt ccgtctcctc ggcgcctggc
     1381 tcgatcgccc acggcgcgcg gagcgctcga gcgctctttt tctgaccgtt ctcgctctcg
     1441 cgctcctcgg gattgctgcc gtgccggttg tgctgaccgt cgtcgtgatt cgctggctct
     1501 tgtaacctca cggcatgtgg cacaagcgca tcgcagtcgg gctcgccctg ctggcggcgg
     1561 cgcttctcgt gctttccggg gtcggcgtgc gtgccggcct gtggccgttc cgcgtgggct
     1621 ttggcatgtt cgccggcgcg ctcgtcgccg gccttgcggc gacggggacc gcgctggtcg
     1681 cgcttctcgt gccgcgcctg cgcgccggcg cggtttccgc gctgctcttc gctctgatcc
     1741 tcggcgcggc gagcgccgcg ctgccgctcg aaaacctgcg gcgcgtgaaa acgcttccgt
     1801 acatcaacga catcacgacg gacacgcaag cgccgccgca gttctcgcag ccccggcctt
     1861 atgagcgcca tttcgcggag ctgcagaagc tcggctaccc cgacctcgta cccctggagc
     1921 tcgccgtgcc gccgcctcaa gccttcgcac gcgcccgcga ggccgcgcac gggctcggct
     1981 gggagatcgt cgccgccgac gaggccgccg gccgcatgga agcggtcgcg acgacgttat
     2041 ggttcggctt caaagacgat gttacggtgc gcattgtgcc gaccgcgaag gggagccgga
     2101 tcgacgtacg gtcgagatcg cgcgtcggcc gcagcgacat cggcgcaaat gccgatcgga
     2161 tccggaaatt tttctcagtg ctgaaaggat gatgtggctc ggcgagggta aaaggggtat
     2221 tcttcaggta tcgtttcatg aaactcggat aggtccatga aacacctcat ctttctagtg
     2281 atcacgggtc ttgccgcaca tgcatgggcg caggcgccgg cgaaagaggc cgccaagccc
     2341 gagccggcga aagaagcggc gaagcccgaa gctgccgagc cccagccggc gaagcatgcg
     2401 gtagtcatcc cgaagaaacg tcgctggcac gaggacgcgc gtcactgcct cgagcggccg
     2461 acgaacaccg acatcatcaa gtgcgcagaa gaatatctct agccggggga aagcctttcg
     2521 gcgcgcccgc aaggtccacc gagaggtaga agtcgctctc cccgcggcgc accaggagcc
     2581 ctagcgttgc gcccgcctgc tggccgagca gacggttgaa ttcctcaagg cttcgcacgg
     2641 gcctgcgctc ccaaaccgta gaccacgtcc ccgagcctga gcccggcccg ctcggcggcg
     2701 cccccggtgc gcaccaccgt caggccgtag ggtagtccca gcgagcgcag cgtcgcgcgc
     2761 ggcaactcac gcaggctgaa tcctgcctcg ccgtcgtgaa tcgtcaccag gcgcagctcc
     2821 gcgtccggcg cgggagagca ggaagccagg cagaccagca aaagcagcgt aacgccacgc
     2881 atggtttcta tacggcgcca ggctcctgca agcgccgcgc cgccgcgggt gcggcgcttg
     2941 ctactgccgg cctggagacg cgccgtgtaa cacgttccgg cgtttataag ttctacagaa
     3001 ggagcgagcc gtgagaaaaa ttcgctttcg ccgccgactt ctcttcattc cttgccttgc
     3061 cctcgcggcg tgcgctaccc ccgatcc
//
LOCUS       DASUNT010000101         3674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_803805, whole genome shotgun sequence.
ACCESSION   DASUNT010000101 DASUNT010000000
VERSION     DASUNT010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803805"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VFX94_02050"
     CDS             <1..619
                     /locus_tag="VFX94_02050"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5766991.1"
                     /translation="AHLANFQLALGVSAKVPAVTLAEYVALVKKDPNAGYYASASAGS
                     IPHFFGVMFARAAGIDMTHVPYKGTAQVLQALAGGEIAAASTLAADIGALARAGKARI
                     LATTGAKRSPAFPDVPTFRESGYDIEGSGWYGLFAPAATPSGFVARLSAAAIDAMRSA
                     TVRGRLESLGVEPTGLGPRELAAVMKRDYEHWGPVIKASGFKADN"
     gene            736..1524
                     /locus_tag="VFX94_02055"
     CDS             736..1524
                     /locus_tag="VFX94_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HEX5766992.1"
                     /translation="MATPPPGTFEQLRRLKLVQSLWMGVERLLADPAFPRGVPLARLV
                     DPGMVAAMSETVLAHVLDWHHHHYVYREQQHARQWHRLKQYLASDRTIGLLGLGELGS
                     DVARKLAALGFNVAGWSRRPKDLPGVTCLTQLEAVLERSDVVVCLLPLTPHTRGILNF
                     RTLRKIKLGGTVLNVARGSHLVAEDLIKVLDSGHLAHAYLDVFEHEPLPPEHPLWKHP
                     AITITPHTAALTEPRTAIPKIVENVERLRRGQPPLNLVDLDAGY"
     gene            1695..1838
                     /locus_tag="VFX94_02060"
     CDS             1695..1838
                     /locus_tag="VFX94_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766993.1"
                     /translation="MERVAEKPSLTLTRCYPVAPEKVWRAWADPQALKQRWAPVSAAF
                     SGT"
     gene            1912..2268
                     /locus_tag="VFX94_02065"
     CDS             1912..2268
                     /locus_tag="VFX94_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEX5766994.1"
                     /translation="MYVDGFVLPVPKKNLKAYRAMATKAGKVWREYGALDYKECVADD
                     VKKGKHTSFPQAVKLKPGEVVWLSWILYRNRRHRDSVNRKVMSDKRITPMMDPKKLPF
                     DGKRFFWGGFKVVVDL"
     gene            2393..2929
                     /locus_tag="VFX94_02070"
     CDS             2393..2929
                     /locus_tag="VFX94_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5766995.1"
                     /translation="MLKWILVGVAAVAAIFAIAVALQPPDFRLERSATMRAPAAEAFA
                     QVNDFHNWRAWSPWEKLDPALKRSYEGPQAGAGATYAWQGNRDVGEGRMTIVESRPGE
                     LVRIKLEFFKPFAATNTAEFSFEPSADGGTAVTWSMSGRNNFLARAICVFVNMDRMVG
                     DMFEQGLAQIKGVVERRS"
     gene            2929..3294
                     /locus_tag="VFX94_02075"
     CDS             2929..3294
                     /locus_tag="VFX94_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5766996.1"
                     /translation="MGNPVVHWELMSKDPAKLSDFYAGIFGWKVQHHPRLNYRAVETG
                     GQGGINGGIVKPDREGPWPGNMLFYILVDDLAKYRKQVVAAGGKIHIEEQKVPGMGKF
                     SLFTDPEGRMMGLWKPKAR"
     gene            3322..>3674
                     /locus_tag="VFX94_02080"
     CDS             3322..>3674
                     /locus_tag="VFX94_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5766997.1"
                     /translation="MNREARALAVRIKRSAAEAYEFLSLPENFPKWASGLGASLRRNG
                     DDWVVETSEGPATVRFSERNAYGVLDHQIRLPRGAAVYVPLRVVANGAGCDLVLTLFR
                     RPEMTEAQFAADAEW"
BASE COUNT          635 a         1230 c         1219 g          590 t
ORIGIN      
        1 cgcgcatctc gcgaatttcc agctcgcgct cggtgtcagc gccaaggtgc cggcggtcac
       61 gctcgccgaa tatgtcgcat tggtgaagaa ggacccgaac gccggctatt acgcctcggc
      121 ttcggcgggc agcatcccgc atttcttcgg cgtgatgttc gcgcgcgccg cgggcatcga
      181 catgacccac gtgccctaca agggcaccgc gcaggtgctg caggcgctcg ccggcggcga
      241 gatcgccgcg gcgagcacgc tcgccgccga catcggtgcg ctcgcgcgcg cgggcaaggc
      301 gcgcatcctc gccaccacgg gcgcgaagcg ctcccccgcg tttcccgacg tgcccacgtt
      361 ccgcgaatct ggctacgaca tcgagggcag cggctggtac ggcctctttg cgcccgccgc
      421 cacgccgagc gggttcgtgg cgcggctctc ggcggcggcg atcgacgcga tgcgctcggc
      481 caccgtgcgc ggccgcctgg agagcctggg tgtcgagccg accggccttg ggccgcgcga
      541 gctcgccgcc gtcatgaagc gcgactacga gcactggggt ccggtgatca aggcgtccgg
      601 cttcaaggcc gacaactgaa catccttttc gcctcgcaga ccgaggaccc ggcgctctgg
      661 ctaccgcggc tgcgccaggc gctgccggcc gaccgctttt ccctttcagc ggatgcggat
      721 atcgacgtcg ccctggtggc gacgccgccg ccgggcacct tcgagcagct caggcgcctg
      781 aagctcgtgc agtcgctctg gatgggtgtc gagcggctgc tcgccgatcc ggcctttccg
      841 cgcggcgtgc cgctcgcgcg cctggtggac ccgggaatgg ttgccgcgat gtccgagacc
      901 gtgctcgcgc acgtcctcga ctggcaccac caccactatg tctaccgcga gcagcagcac
      961 gcccggcaat ggcaccggct caagcagtat ctcgcctccg accgcacgat cgggctcctc
     1021 gggctcggcg agctcggcag cgacgtcgcg cgcaagctcg ccgcgctcgg cttcaacgtc
     1081 gcgggctgga gccgccggcc gaaagacctg cccggcgtaa cgtgcctcac ccagctcgaa
     1141 gcggtgctcg agcgaagcga cgtggtggtc tgcctgctgc cgcttacccc gcacacccgg
     1201 ggcatcctga atttccggac cctgcgcaag atcaagctcg gcggcaccgt cctcaacgtc
     1261 gcccgcggca gccacctcgt cgccgaggac ctgatcaagg tgctcgactc cggccacctc
     1321 gcccacgcgt acctcgacgt cttcgagcac gagccgctgc cgccggagca tccgctgtgg
     1381 aagcaccccg cgatcaccat caccccgcac accgctgctc tcaccgagcc gcgcaccgcg
     1441 atccccaaga tcgtcgagaa cgtcgagcgc ctgcgccgcg gccagccccc gctcaacctc
     1501 gtcgacctcg acgccggcta ctgaagaaaa gtatcaggat gcggagctcg acaggaaggg
     1561 cgcgtagtga gctgcgagct ttccgccgcg ccgatgcagg ccgcggcggc gtggatgtcg
     1621 cgctacgaga agttctggac tgagaagctc gattctctgg cccgctatct ctccccgcgg
     1681 gaggagttgc agacatggaa agagtcgccg agaagccctc gctcaccctc acccgctgct
     1741 accccgtcgc gccggagaaa gtctggcgcg cgtgggccga cccgcaggcg ctgaagcaac
     1801 gctgggcgcc ggtctcggct gctttctcag gcacttagcg ctgctgtcga aatccgccgc
     1861 gctcgttcga ctacaggcat cggccctgtc gggcctattc aggaggaaag catgtacgta
     1921 gacggattcg tattgccggt accgaagaag aatctgaagg cctatcgcgc catggcgacc
     1981 aaggcgggca aggtgtggcg cgagtacggc gctctcgact acaaggagtg cgtcgccgac
     2041 gacgtgaaga agggcaagca cacctccttc ccgcaggctg tgaagctcaa gcccggcgag
     2101 gtggtctggc tctcgtggat cctctatcgc aaccgcaggc accgcgacag cgtgaaccgc
     2161 aaggtgatga gcgacaagcg catcacgccg atgatggacc cgaagaagct gccattcgat
     2221 ggcaagcggt ttttctgggg cggattcaag gtggtggtgg acctatgagg tcgcaagcgg
     2281 gatgaacgag cacgcggtca ggttcactcg cgtttcgagc ggcttcacgg gctgatccaa
     2341 agggatgggc gcagacggtg gagcgccgcg acacgcttct gaggagatga agatgctcaa
     2401 gtggatactc gtcggggttg ccgcagtggc ggcaatcttc gcgatcgcgg tcgcgctgca
     2461 gcctcccgac ttccgcctcg agcgcagcgc caccatgcgc gctccggcgg ccgaggcctt
     2521 cgcccaggtc aacgatttcc ataactggcg cgcatggtcg ccgtgggaaa agctagaccc
     2581 ggcgctcaag cgcagctacg aggggcctca ggccggagcc ggcgcgacgt acgcctggca
     2641 aggcaaccgg gacgtcggcg aaggccgcat gacgatcgtg gagagccgtc ctggcgagct
     2701 tgtgcgcatc aagctcgagt tcttcaagcc gttcgccgcc accaacactg cagagttcag
     2761 cttcgagccg agcgccgatg gcggcaccgc cgtcacctgg agcatgagcg gccggaacaa
     2821 ttttctcgcg cgtgcgattt gcgtgttcgt gaacatggac cgcatggtgg gcgacatgtt
     2881 cgagcagggg ctcgcgcaga tcaaaggcgt ggtggaaagg aggtcgtgat gggcaacccc
     2941 gtagtgcatt gggagctgat gtcgaaggac ccggccaagc tgtcggactt ctacgccggc
     3001 atcttcgggt ggaaagtgca gcatcatccc cggctgaact accgcgccgt ggagacggga
     3061 ggacaaggag gaatcaacgg cggcatcgtc aagccagacc gggaaggtcc ctggccaggg
     3121 aacatgctct tctacattct ggtcgacgac ctcgcgaagt accggaagca agtcgtggcc
     3181 gcgggcggca agatccacat cgaggagcaa aaggtccccg gcatgggcaa gttctcgctt
     3241 ttcaccgacc ccgagggccg catgatgggc ctgtggaaac cgaaggcccg atgaagcgca
     3301 cctaccttta ggtcgcaggc catgaaccgg gaagctcgcg ccctggccgt tcggatcaag
     3361 cgttccgcgg cggaggcgta tgaattcctt tcgctgcccg agaacttccc gaagtgggcg
     3421 tccgggctgg gcgcgtcgct gcggcgaaat ggagacgact gggtagtcga aacctccgag
     3481 ggcccggcca ccgtgcgatt ctcggagcgc aacgcctatg gcgtgctcga tcaccagatc
     3541 aggctgccgc gtggcgcagc ggtttacgtg ccgctgcgcg tggtcgcgaa cggcgcgggc
     3601 tgcgatctcg tcctgaccct ctttcgccgg cccgagatga ccgaagctca gttcgccgcc
     3661 gacgccgaat ggtt
//
LOCUS       DASUNT010000102         2952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1067183, whole genome shotgun sequence.
ACCESSION   DASUNT010000102 DASUNT010000000
VERSION     DASUNT010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067183"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFX94_02085"
     CDS             <1..198
                     /locus_tag="VFX94_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5766998.1"
                     /translation="LASLLPAAALAGAIGGAALCAFGWMGTTTLLVSLPGAERAYPVR
                     GRNTMLLDFAEALSERLMLRK"
     gene            227..1687
                     /gene="ubiD"
                     /locus_tag="VFX94_02090"
     CDS             227..1687
                     /gene="ubiD"
                     /locus_tag="VFX94_02090"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041322210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEX5766999.1"
                     /translation="MQYSDLRQFVSQLEASGELKRIAAEVSPRLEMTEICDRVLRAGG
                     PALLFERPAGHDIPVLANLFGSVRRIAAAMGLADTRALREVGKMLAFLREPEPPRGLR
                     DLWEKFPVWKQLLNLPPKVVSKALCQESVLEGKDVDLARLPVQTCWPQDAGPLITWGL
                     TVTRGPAKPRQNLGIYRQQVLGRDRVIMRWLAQRGGALDFRDHVAASKEPFPLAVALG
                     ADPATMLAAVTPIPDALSEYQFAGLLRGARTELVKCLGSSLEVPASAEIVLEGVIHQS
                     DTAEEGPHGDHTGYYNEVERFPVMTVQRITMRRSPIYHSTYTGKPPDEPAMLGVALNE
                     VFVPLLQKQFPEIVDFYLPPEGCSYRLAVVSMKKQYPGHAKRVMFGVWSYLRQFMYTK
                     FIVVCDDDIDVRDWSEVIWALTTRVDPGRDSVVVERTPIDYLDFASPVSGLGSKMGID
                     ATHKWPAETSRRWGRPIVMEEAVKRRVDSLWRELGL"
     gene            complement(1684..>2952)
                     /gene="dacB"
                     /locus_tag="VFX94_02095"
     CDS             complement(1684..>2952)
                     /gene="dacB"
                     /locus_tag="VFX94_02095"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX5767000.1"
                     /translation="VGASRASLSLNAGSSRNPGSVIKLVTTYAALELLGPAYRWKTEA
                     YVEGEDLYLKGYGDPRLDYESFWMLLRNLRGRGVRDIRGDIVLDRSYFAPGTPELIDN
                     EVFRPYNVAPDALLVNFKSLRFVFWPHEAAVRMFVEPQLPGVEIVNGLRLSDGACPEG
                     RAFRERIQANFQSKPPRAAFSGFYPVACGERELSVALHQPEDYVDAMVRALWAEMGGQ
                     WHGRIREGEVGPNARLLYKHESAALSELVRDINKFSNNVMARQLYLTLAAELGGPPGE
                     SQAAARAIEQWLLFKGIQAEELRLENGSGLSRHERASAVTLMALLQAAWKSAVMPEFI
                     ASLPVVSADGTMRRRLHFQGVSGNAHIKTGLLSDTRSIAGYVLDRSGRRHAVVMIANH
                     PNAPYADAAFDALLQSVRASAREEQHQKSR"
BASE COUNT          480 a          968 c         1006 g          498 t
ORIGIN      
        1 ctcgcgagcc tgctcccggc agcggcgctc gccggcgcga tcggcggagc ggccctctgc
       61 gcgttcggat ggatggggac cacgacgctt ctcgtcagcc tgccgggcgc cgagcgcgcc
      121 tacccggtgc gcgggcgcaa caccatgctg ctcgacttcg ccgaggcgct gagcgagcgc
      181 ttgatgctca ggaagtagga aggcgcttgc ggtacggaga aaacccttgc agtactcgga
      241 cctgaggcaa ttcgtttcgc agctcgaggc gagcggcgag ctcaagcgca tcgccgccga
      301 agtgtcgccg cgcctggaaa tgaccgagat ctgcgaccgt gtgctgcgcg cgggcggccc
      361 ggctctgctc ttcgagcggc ctgccgggca cgacattccg gtgctcgcga atcttttcgg
      421 ctccgtgcgc cggatcgccg cggcgatggg gctcgccgat acgcgcgctt tgcgcgaagt
      481 cggaaagatg ctcgccttcc tgcgcgagcc ggagccgccc agggggctgc gcgacctttg
      541 ggagaagttc cctgtatgga agcagctgct caatctgccg cccaaggtgg tgtcgaaggc
      601 gctttgccag gagagcgtcc tcgaaggaaa ggacgtcgac ctcgcgcgcc tgccggtgca
      661 gacgtgctgg ccgcaggacg cgggtccgct cattacctgg ggcctcacgg tgacgcgcgg
      721 tccggccaag ccgcgccaga acctcgggat ctatcgtcag caggtgctcg gccgcgaccg
      781 cgtgatcatg cgctggctcg cgcagcgcgg cggcgcgctc gattttcgcg atcacgtcgc
      841 agcgtccaag gaaccgtttc ccttggcggt cgcgctgggc gcagacccgg ccacgatgct
      901 cgcggctgtg acgccgatcc cggatgcatt gtcggagtac cagttcgcgg gactactgcg
      961 cggagcgcgc accgagctcg tcaagtgtct cgggagcagc ctcgaggtgc cggcgagcgc
     1021 cgagatcgtt ctcgaaggcg tcatccacca gtcggatacc gcggaagagg ggccgcacgg
     1081 cgaccatacc ggctactaca acgaagtcga gcgcttcccg gtgatgacgg tgcagcggat
     1141 cacgatgcgc cgctcgccca tctaccactc cacctatacc ggcaagccgc ccgacgagcc
     1201 cgccatgctc ggtgtcgcgc tgaacgaggt gttcgtgccc ctgctgcaga agcaattccc
     1261 ggagatcgtg gacttctatc tgccgccgga agggtgctcg tatcgcctcg ctgtggtgag
     1321 catgaagaag cagtacccgg ggcacgccaa gcgcgtgatg ttcggcgtct ggagctacct
     1381 gcgccagttc atgtacacca agttcatcgt ggtgtgcgac gacgacatcg atgtgcgcga
     1441 ctggagcgaa gtgatctggg cgctcacgac gcgcgtcgat cccggccgcg actcggtggt
     1501 ggtcgagcgc acgccgatcg actacctcga ttttgcgagc cccgtgtcgg ggctcggctc
     1561 gaagatgggc atcgatgcaa cccataaatg gccggcggaa acgtcgcggc gctggggccg
     1621 cccgatcgtc atggaggagg ccgtgaagcg ccgcgtggac tccctgtggc gggagctggg
     1681 cctttagcgc gacttctggt gctgctcttc cctggcagag gcgcgcaccg actgaagcag
     1741 cgcatcgaac gccgcgtccg cgtagggcgc gttggggtgg tttgcgatca tgaccacggc
     1801 gtggcgccgg ccgctgcggt cgaggacgta cccggcgatc gagcgcgtat ccgaaagcag
     1861 cccggtcttg atatgggcgt tgcccgagac cccttgaaaa tgcaggcgcc tgcgcatcgt
     1921 gccgtccgcg gaaacgaccg gtagtgaagc gatgaactcc ggcattaccg cgctcttcca
     1981 ggcggcttgc agcagcgcca tcaacgtcac ggcgctcgcg cgctcgtggc gcgaaagccc
     2041 cgagccgttc tcgaggcgca gctcctcggc ctggatgccc ttgaagagga gccattgctc
     2101 gatcgcgcgc gccgcagcct gcgactcgcc cggcgggccg ccgagctcgg cggcgagcgt
     2161 caggtagagc tggcgcgcca tcacattgtt ggagaacttg ttgatgtcgc gcacgagctc
     2221 gctgagcgcc gcggactcgt gcttgtagag cagccttgca ttcgggccca cctcgccttc
     2281 gcggatgcgt ccgtgccatt ggccgcccat ctcggcccac agggcacgta ccatggcgtc
     2341 gacgtagtcc tcgggctgat gcagcgccac gctgagctca cgttcgccgc atgcgaccgg
     2401 gtaaaagccg ctgaacgccg cgcgcggcgg cttcgactgg aaatttgcct ggatgcgctc
     2461 gcggaaggcc cgtccttcgg gacaggcgcc gtccgacagt ctcagtccgt tgacgatctc
     2521 gactcccggc aattgcggct cgacgaacat gcgcaccgcg gcctcgtgag gccagaacac
     2581 gaagcgcagg gatttgaaat tgacgagcag cgcgtccggc gcgacgttgt acgggcggaa
     2641 cacctcgttg tcgatcagct ccggcgtccc gggcgcgaaa tagctgcgat cgagcacgat
     2701 gtcgccgcgg atgtcgcgca ctccgcggcc acgcaggttt cgcagcagca tccagaagct
     2761 ctcatagtcg agcctcgggt cgccgtaacc cttgaggtag agatcctcgc cctcgacata
     2821 ggcttcggtc ttccagcggt aggccgggcc gagaagctcc agcgcggcat aggtggttac
     2881 cagctttatc accgagcccg ggttgcgcga gctgcccgca ttgagcgaaa gcgaggcgcg
     2941 tgacgcaccg ac
//
LOCUS       DASUNT010000103         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_540926, whole genome shotgun sequence.
ACCESSION   DASUNT010000103 DASUNT010000000
VERSION     DASUNT010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540926"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFX94_02100"
     CDS             <1..240
                     /locus_tag="VFX94_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767001.1"
                     /translation="RPARGGRRHSLRGSGPALSMDIVYFTIIAIGLYFGADWLLDRIE
                     RARGKRLDNRQVVFFAIILPLAVATFWLVRMLSGR"
     gene            complement(249..1283)
                     /locus_tag="VFX94_02105"
     CDS             complement(249..1283)
                     /locus_tag="VFX94_02105"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX5767002.1"
                     /translation="MTDRDRSIVAAWLFVCAALAFAVVLVGGITRLTRSGLSIVEWQP
                     LIGIVPPLSAQDWEALFARYRETPEFRQLNPNMTLEGFKGIFWWEYTHRLLARVIGIV
                     YVVPLLFFQWRKMLERSLAVKLWAIFVLGALQGVLGWYMVKSGLIDDPQVSPVRLSAH
                     LGLALVIFAAELWIALQLLSPRIRPFEKIPLLLPYLVLAMAVSGGMVAGLRAGYAYNT
                     FPLMNGELVPPDLLTLEPWWRNFLYNMASVQFLHRAFFWLIALLVLVSWWPARHRAAS
                     HVLLLAFAAQAALGILTLLNAVPLPLAVAHQSGAVVLLAAALWNAHSAGTWPAQRRIG
                     GQDPFPRKGS"
     gene            1375..1548
                     /locus_tag="VFX94_02110"
     CDS             1375..1548
                     /locus_tag="VFX94_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767003.1"
                     /translation="MSAFKKSLIAAGAAASLAVSANIAAQAVQTDAYATSGPANQIWK
                     NPFGLCWRTINER"
     gene            complement(1563..>2010)
                     /locus_tag="VFX94_02115"
     CDS             complement(1563..>2010)
                     /locus_tag="VFX94_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003276206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX5767004.1"
                     /translation="QLAANSSGVAAVRVSLAGFDTHQNQPNIHARLLGELATGLVALK
                     GALGEAKRWDSTLVMTYAEFGRRPQENNSGGTDHGTAAAHFITGGRVKGGLYGDAPHL
                     DQLEGGNLRHAVDFRSVYATALERWWGVSSARALGGRFAPLDVLRA"
BASE COUNT          375 a          654 c          671 g          310 t
ORIGIN      
        1 cggcctgctc gcggtggccg gcggcattct cttcgcggta gtggtcctgc gctgtctatg
       61 gacatcgtct acttcacgat catagcgatc ggcctttact tcggcgccga ctggctcctc
      121 gaccgcatcg agcgcgcgcg cggcaagcgc ttggacaacc gccaggtggt gttcttcgcc
      181 atcatcctgc cgctcgcggt cgcgaccttc tggctggtcc ggatgctttc gggccgctga
      241 aaaaggggtc aggacccttt tcgcggaaaa gggtcctgac cccctatgcg ccgctgcgcc
      301 ggccaggtgc ctgccgagtg tgcattccat agcgccgcgg cgagcagcac caccgccccg
      361 ctctggtgcg cgacggcgag gggaagcggc acagcgttaa gcagcgtcag gatgccgagc
      421 gccgcctgag ccgcgaatgc gagcagcagc acgtggctcg ccgccctgtg gcgcgcaggc
      481 caccaggaga cgagcacgag cagggcaatg agccagaaga acgcccggtg caggaactgg
      541 acgctcgcca tgttgtagag aaagttccgc caccacggct cgagcgtcag cagatccgga
      601 ggcacgagct cgccattcat gagagggaag gtgttgtagg cgtagccggc gcgcagcccg
      661 gcgaccatgc cgccggacac cgccatggcg agcaccaggt aggggaggag gagcgggatc
      721 ttttcgaacg ggcggatccg gggtgaaagg agctgcagcg cgatccagag ctcggcggcg
      781 aagatcacca gcgcgagccc gaggtgggcg ctgagccgca ccgggctcac ctggggatcg
      841 tcgatgagcc cggacttcac catgtaccag ccgagcacgc cctgcagcgc gccgaggacg
      901 aaaatcgccc agagcttcac cgcgagcgag cgctcgagca tcttgcgcca ctggaaaaag
      961 agcagcggca ccacgtagac gatgccgatc acccgcgcga gaaggcggtg ggtgtactcc
     1021 caccaaaaaa tgcccttgaa gccctcgagc gtcatgttgg ggtttaactg gcgaaattcc
     1081 ggtgtttcgc ggtacctggc gaaaagcgct tcccagtctt gcgccgaaag cggcggcacg
     1141 atcccgatga gcggctgcca ctcgacgatg gaaagacccg agcgtgtcag gcgggtgatc
     1201 ccccccacaa gcaccacggc gaacgcgagc gcggcgcaga cgaaaagcca ggcggcgacg
     1261 atgcttcgat cgcgatcggt catgcgcaca tcatgccccg attggtatat gtttgccttg
     1321 gaaacccagt aagaatgcac ggccttccat accaacaatc cgggaggatt catcatgagt
     1381 gcgttcaaaa agtccttgat tgcggcggga gctgcggctt cgctcgcagt ctcggcgaac
     1441 atcgcagcgc aggcggtcca gaccgatgcc tatgccacca gcggccccgc aaaccagatc
     1501 tggaaaaacc cgttcggcct ttgctggcgg acgatcaacg agcgttaagg cgcgagcgcc
     1561 gctcaggcgc gcagcacgtc gagcggcgcg aaccgcccgc ccagcgctct tgcgctcgag
     1621 acgccccacc agcgctcgag cgcggtggcg taaacgctgc ggaagtccac tgcgtggcgg
     1681 aggttcccgc cctcgagctg gtccaggtgc ggcgcgtcgc cataaaggcc gcccttaact
     1741 ctgccacccg tgatgaagtg cgcggccgcg gtgccgtggt cggtgccgcc cgaattgttt
     1801 tcctgcggcc ggcggccgaa ctcggcgtag gtcatgacga gcgtcgagtc ccagcgcttt
     1861 gcctcgccga gcgcgccctt cagcgcgacg aggcccgttg caagctcgcc gagaagccgc
     1921 gcgtggatgt tgggctggtt ctggtgcgta tcgaacccgg ccagcgacac gcgaaccgcc
     1981 gcgacgccgg aagagttggc cgcgagctgc
//
LOCUS       DASUNT010000104         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_102897, whole genome shotgun sequence.
ACCESSION   DASUNT010000104 DASUNT010000000
VERSION     DASUNT010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102897"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VFX94_02120"
     CDS             complement(<1..866)
                     /locus_tag="VFX94_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5767005.1"
                     /translation="MPHAMTDDGVKLYYEEAGEGKPLIFVHEYAGDYRSWEPQMRLFS
                     RWYRCIAFNARGYPPSDVPEDPEKYSQARARDDIRSVLDHLRIDKAHVCGLSMGGFAA
                     LHFGIAYGARAVSLVVGGCGYGAEPAKREQFRAETNAAAEQIEKQGMREVAKRYSLGP
                     TRVQFQNKNPRAWREFAEQLAEHSTLGSALTMRGVQARRPSLWELVEDMKRIEVPTLV
                     VTGDEDDPCLEPAILMKRSIATAALAVLPSAGHTINLEDPEEFNRVLFNFLAAVDMGT
                     WRRRDPRSTSGG"
     gene            complement(871..1614)
                     /locus_tag="VFX94_02125"
     CDS             complement(871..1614)
                     /locus_tag="VFX94_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HEX5767006.1"
                     /translation="MNLELKGKSVLITGGSKGIGLACAKAFAAEGCRVHIASRNKETL
                     EKAAQAIGGETRVHGADLRDGKALRALAEACADVDILVNNAGDIPGGTIEAIDEAKWR
                     HAWELKVFGYVNLTRELFARMKARKSGVIVNVIGMAGEHPSFEYVCGSMANAGLGAFT
                     KAMGKGSAAFGVRVLGVHPPATRTDRIIPLMKAAAREKLGDENRWQELVGTGSFGQMI
                     EPEQVADMVAFLASARAGKLSGIMINLGA"
     gene            complement(1611..>2586)
                     /locus_tag="VFX94_02130"
     CDS             complement(1611..>2586)
                     /locus_tag="VFX94_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HEX5767007.1"
                     /translation="GLHGRSDSQRDFLMIGPEERYDFAHFEFVMRGVQAARERFRDKA
                     GQISLSLHCETAEIMTAYTKIVESEGKLDGLAAYHAARPPHSEGLAVFIASYLANEAA
                     LPNINLLHLSSRKALEAALKMAEVFPHIDFRREVTIGHLVLDVNSKAGVLAKVNPPIR
                     PREDVEFLWEKVLEEKVDWVVSDHACCRHETKAAKHALGNIWLAKSGFGGTEYLLAAL
                     VSEGTKRGLSLNRVAALTSGNPAKRFGLNSKGDIAPGLDADLALVDPKHTWTIRAKDS
                     ESTQGYTPFEGIELSAKVKATFLRGSLICENGNVLGKPKGRYMHRPTP"
BASE COUNT          417 a          860 c          831 g          478 t
ORIGIN      
        1 atgccgccgg aggtcgagcg cggatcgcgc cggcgccacg tgcccatgtc cacggcggcg
       61 aggaagttga acagcacgcg gttgaactcc tcgggatcct cgaggttgat cgtgtggccc
      121 gcgctcggca gcacggcaag cgccgccgtt gcgatcgaac gcttcatgag gatcgcgggc
      181 tcgaggcacg ggtcgtcctc atcgccggtc acgacgagcg tcggaacttc gatgcgcttc
      241 atgtcctcga cgagctccca gagcgacggc cggcgtgcct gcacgccgcg catggtgagc
      301 gccgagccga gcgtcgagtg ctcggcaagc tgctcggcga actcgcgcca cgcgcgcggg
      361 ttcttgttct ggaattgcac gcgcgtcggg ccgagggagt agcgcttggc cacctcgcgc
      421 atgccttgct tttcgatctg ctcggcggcg gcattggtct ccgcgcggaa ctgctcgcgc
      481 ttcgccggct cggcgccata gccgcagccg ccgacgacca gcgacacggc gcgcgcgccg
      541 tacgcgatgc cgaagtgcag cgccgcgaag ccgcccatgg agaggccgca gacatgcgcc
      601 ttgtcgattc tcaggtgatc gagcaccgat cggatatcgt cgcgcgcgcg cgcctgcgag
      661 tatttttccg gatcctccgg cacgtcggac ggcgggtagc cgcgcgcatt gaaggcgatg
      721 cagcgatacc agcgcgagaa gagccgcatc tgcggctccc agctgcggta gtcgcccgcg
      781 tactcgtgca cgaagatgag cggtttgcct tcgccggcct cctcgtaata cagcttgacg
      841 ccatcgtcgg tcatggcatg cggcatgcgc tcaggctccg aggttgatca tgattccgga
      901 aagcttgccg gcgcgcgccg aggcgaggaa ggccaccata tcggcgacct gctcaggctc
      961 gatcatctgc ccgaaggagc cggtaccgac cagctcctgc cagcggtttt cgtcgccgag
     1021 cttttcccgg gcggcggctt tcataagcgg gatgatgcgg tcggtgcgcg tcgccggcgg
     1081 atgcacgccg agcacgcgca cgccaaacgc agccgagcct tttcccattg ccttggtgaa
     1141 ggcgcccagc ccggcgttcg ccatgctgcc gcacacgtat tcgaacgaag gatgctctcc
     1201 cgccatgccg atgacgttca cgatcacgcc gctcttgcgc gccttcatgc gcgcgaaaag
     1261 ctcgcgcgtg aggttgacgt agccgaagac tttcagctcc cacgcgtggc gccacttcgc
     1321 ctcgtcgatc gcctcgatcg tgccgccggg aatgtcgccc gcgttgttca ccaggatgtc
     1381 gacatctgcg cacgcctcgg cgagcgctcg cagcgccttg ccgtcgcgca ggtcggcgcc
     1441 atgcacgcgc gtttcgccgc cgatcgcctg cgcggccttt tcgagcgttt ccttgttgcg
     1501 cgatgcgatg tggacgcggc agccttcggc ggcgaacgct ttcgcgcacg cgaggccgat
     1561 gcccttcgag ccgccggtaa tgagaacgga cttgcctttc agttcgaggt tcatggggtc
     1621 ggacgatgca tgtaacggcc tttcggtttg ccgagcacgt tgccgttctc gcagatcagg
     1681 ctgccgcgca gaaaagtcgc cttcaccttc gccgagagct cgatgccctc gaacggcgta
     1741 tatccctgag tggactccga atccttcgcg cggatggtcc acgtgtgctt cggatcgacg
     1801 agcgcgagat cggcgtccag gcccggcgcg atgtcgccct tggagttcaa gccaaagcgc
     1861 ttcgcggggt taccgctggt cagcgcagcc accctattca gggaaagccc tcgttttgtc
     1921 ccctcggaca caagcgcggc cagcagatac tccgtgcccc cgaaacccga tttggcaagc
     1981 cagatgttgc cgagcgcgtg ttttgccgcc ttggtctcat gccggcagca ggcatggtcg
     2041 ctcaccaccc agtcgacctt ctcctcgagc accttctccc agaggaactc cacgtcctcg
     2101 cgcgggcgga tgggaggatt gaccttggcg agcacgcctg ccttggagtt cacgtccagg
     2161 acgaggtgac cgatggtgac ctcgcgacga aaatcgatat gcggaaacac ctccgccatc
     2221 ttgagcgctg cttccagcgc ttttctggag gagaggtgga gcaggttgat gttgggaagc
     2281 gccgcctcgt tcgcgagata ggaggcaatg aagaccgcga gtccctccga atgcggcggc
     2341 cgcgccgcgt ggtaggcggc aaggccgtcc agcttgcctt cgctctccac gatctttgta
     2401 tacgccgtca tgatctcggc ggtctcgcaa tgcagcgaca gactgatctg tcccgccttg
     2461 tcgcgaaagc gctcgcgcgc cgcctgcacg ccgcgcataa caaactcgaa gtgcgcgaag
     2521 tcgtagcgct cctcggggcc gatcatcaaa aagtcgcgct ggctgtcgga acggccgtgc
     2581 aatccg
//
LOCUS       DASUNT010000105         2128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_979588, whole genome shotgun sequence.
ACCESSION   DASUNT010000105 DASUNT010000000
VERSION     DASUNT010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979588"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /gene="gspK"
                     /locus_tag="VFX94_02135"
     CDS             complement(<1..913)
                     /gene="gspK"
                     /locus_tag="VFX94_02135"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HEX5767008.1"
                     /translation="MHRQRGAAVIVAMLIVALAATAAVAALQQQDLALRQLTTARDYE
                     QASWVLKGGAHWARSILFQDGRFSAIDHGGELWASGLPRTEIEQGTVSGEISDQQGLF
                     NVNNLALEKNASARDIEAFRRLLGSVGLNPDLAQAIADWIDADEEALPVAGAEDDYYL
                     RLPAPYRAANQPVVELGELLRVRGMDEASLARLRGFAAALPRRTPVNVNLAPPELLFA
                     MVPGLTLEEARALASTRVQSPFRSLEEFEKRLPRRQLKWIEGTLSVGSNYFVVRGRAT
                     VGKADVRMEALLQRERGAMPVVLWQRMR"
     gene            complement(919..1509)
                     /gene="gspJ"
                     /locus_tag="VFX94_02140"
     CDS             complement(919..1509)
                     /gene="gspJ"
                     /locus_tag="VFX94_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HEX5767009.1"
                     /translation="MRQRGFTLLELLVAIAVLAVMGALGFRTLGSVLEAESRLQAETR
                     RWSDLSLLFSQLGEDMTMAVERPVRALAGRTSPALSLEQSTDPVHPHLVLTRLGMGEG
                     SAAQSAPRRVGYRLREGKLEYLIWPSPDGQPGVQPAAYVALEHVAAAQWQALDAEGRW
                     VNAWPAGPAANALPRAISLRLTLAGGEEVTRVLALR"
     gene            complement(1511..1876)
                     /gene="gspI"
                     /locus_tag="VFX94_02145"
     CDS             complement(1511..1876)
                     /gene="gspI"
                     /locus_tag="VFX94_02145"
                     /inference="COORDINATES: protein motif:HMM:NF014551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HEX5767010.1"
                     /translation="MTKARGFTLVEVLVALAIVAVALAAVSRSVALSTDSAFEHKLRV
                     LSGVVAENRMAELAARRAWPGIGLTEGTERQGGLEFRWRAEVFATPHPQLRRVEIRVN
                     TPDDRHELRRLVGVLSRDF"
     gene            complement(1845..>2128)
                     /locus_tag="VFX94_02150"
     CDS             complement(1845..>2128)
                     /locus_tag="VFX94_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767011.1"
                     /translation="QGEDGEWQGFPAESPYRRRSLPAGVSLGAVQLNAQPLRSGERVV
                     ITPYGLGDAIQATIFGGGASITLRGGALGRVTLDSSALFHDESARIYSG"
BASE COUNT          329 a          757 c          737 g          305 t
ORIGIN      
        1 atcgcatgcg ctgccagagt accaccggca tggcgcctcg ctcgcgctgc agcagcgcct
       61 ccatgcgcac gtccgctttg cctacggtcg cccggccgcg gaccacgaag tagttgctgc
      121 caaccgagag cgtgccctcg atccatttca attggcgccg cggcagcctc ttttcgaact
      181 cttccaggct gcggaacggc gactggacgc gggtgctcgc gagcgcgcgg gcttcctcga
      241 gcgtcagccc ggggaccatg gcgaagagca gctccggggg cgcgaggttc acgttcaccg
      301 gggtgcgccg cggcagcgcc gcagcaaagc cgcgcaagcg tgcgagggaa gcttcgtcca
      361 tgccgcgcac gcgcagcagc tcgccgagct ccaccacggg ctggttcgcc gcgcgatacg
      421 gcgcggggag gcgcaagtag tagtcatcct cggcaccagc gacgggcagc gcttcctcgt
      481 cggcgtcgat ccagtcggcg atcgcctgcg cgagatcggg gttcagcccg acgcttccca
      541 gcaagcgccg aaacgcctcg atgtcgcgcg cgctcgcgtt cttttcgagc gcgagattgt
      601 tgacgttgaa caggccctgc tgatcgctga tctcgccgga cacggtcccc tgctcgatct
      661 cggtgcgagg caggccgctt gcccaaagct cgccgccgtg atcgatcgcg gagaagcgtc
      721 catcctgaaa gagaatcgag cgcgcccagt gcgcgccgcc cttgagtacc cagctcgcct
      781 gctcgtagtc gcgcgcggtc gtgagctgcc gcagcgcaag gtcctgctgc tggagcgccg
      841 ccacagcagc cgtggccgcg agggctacga tcagcatggc gacgatgact gcggcgccgc
      901 gctggcggtg catggcgctc atctcagggc gagcacgcgc gtcacttctt cgccgcccgc
      961 gagcgtgagc cggagcgaga tcgcgcgcgg cagtgcgttc gcggcgggtc ccgccggcca
     1021 ggcgttcacc cagcggccct cggcatcgag tgcctgccac tgcgcggctg cgacatgctc
     1081 gagagccacg tacgcggcag gctgcacgcc cggctggccg tcgggcgacg gccagatgag
     1141 gtactcgagc tttccttcgc gcaggcgata gcccacgcgg cgcggggcgc tttgcgccgc
     1201 ggagccttcg cccattccca ggcgcgtgag gacgagatgg ggatggaccg ggtccgttga
     1261 ctgctcgagg ctcagtgccg ggctcgtgcg cccggcgagc gcgcgcacag ggcgctcgac
     1321 tgccatggtc atgtcctcgc cgagctgcga gaaaagcagc gagagatcgc tccagcggcg
     1381 cgtctccgcc tgcaggcgcg attccgcctc gagcaccgag ccgagcgtcc ggaagccgag
     1441 cgcacccatc accgccagca cggcgatggc gacgaggagt tcgagaagcg tgaacccgcg
     1501 ctggcgcatg ctagaagtcc ctcgagagca cgccgaccag gcggcgcaac tcatggcgat
     1561 cgtccggagt gttcacccgg atctcgacgc ggcgaagctg cggatgcgga gtggcgaaca
     1621 cctccgcgcg ccagcggaac tcgagcccgc cctggcgctc ggtgccttcg gtcaatccga
     1681 ttccgggcca cgcgcgccgc gcggcgagct ccgccatgcg gttctcggct accacgccgg
     1741 aaagcacgcg cagcttgtgc tcgaacgcac tgtcggtgga gagcgcgacc gatcggctca
     1801 ccgcggcaag cgcgacggcg acgatggcga gcgccaccag cacctcaacc agagtaaatc
     1861 cgcgcgcttt cgtcatggaa aagggcgctg ctgtcgagcg tgacgcggcc gagcgcgccg
     1921 ccgcgcaaag tgatgctcgc gccgcccccg aagatcgtcg cctggatcgc gtcacccagg
     1981 ccgtagggcg tgatgacgac gcgctcgccg gagcgcagcg gctgcgcatt gagctgcact
     2041 gccccgagcg atacgcccgc cggcagcgac cggcgccggt aggggctctc ggcgggaaac
     2101 ccctgccact cgccgtcctc gccttggc
//
LOCUS       DASUNT010000106         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_541361, whole genome shotgun sequence.
ACCESSION   DASUNT010000106 DASUNT010000000
VERSION     DASUNT010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541361"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFX94_02155"
     CDS             complement(<1..196)
                     /locus_tag="VFX94_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEX5767012.1"
                     /translation="MKKLKFAFLLSSLAASATLFAQSTTPAQSPAAKAPAVRPAQVAQ
                     APAAGGQAAGAAAGQTSAGTG"
     gene            complement(316..702)
                     /locus_tag="VFX94_02160"
     CDS             complement(316..702)
                     /locus_tag="VFX94_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767013.1"
                     /translation="MRNLLAVAAALAAGCSIIGHQKVEGWPELEIVEHYVPHAEMRDR
                     CKPYVGFGMEPAACAEFDLARGKCHIWFSADFPPQKFIVDHERLHCAGYDHIGSTNMQ
                     RFLAGHRATQAAVGAVAGGPGLSPRP"
     gene            787..2070
                     /gene="hmgA"
                     /locus_tag="VFX94_02165"
     CDS             787..2070
                     /gene="hmgA"
                     /locus_tag="VFX94_02165"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX5767014.1"
                     /translation="MPSTRITYQSGFGNTFSSEALKGALPVGRNSPQIPPKGLYAEVL
                     SGTAFTAPRAENQSSWLYKLRPSAMHGPYKRIKEGFLRSGPFDEVETPPNRLRWDPLP
                     MATKPTDFLQGLATMAGSGVPAMQVGLAVHIYRANRSMNGRYFWNADGELMFVPQEGA
                     LLIFTELGKLQVKPGEIAVVPRGMKFQVSVEGPVRGYLCENYGAAFRLPELGPIGSQG
                     LAQPRDFQAPAAAYEDGGKCEVVAKFMGGLWAADFAHSPLDVVAWHGDYVPYKYDLAR
                     FMTIGTISFDHPDPSLFTVLTSPSAQPGVANCDFVIFPPRWLVAEDTFRPPWFHRNVM
                     SELMGLVHGVYDAKAEGFLPGGVSIHNCMTAHGPDVATWEKASAAKLEPQKITDTLAF
                     MWESRYVFRPTKFAMTARELQKNYDAVWSGFRKNL"
BASE COUNT          365 a          729 c          720 g          350 t
ORIGIN      
        1 taccggttcc cgcgctcgtc tggccggctg cggcgcccgc agcctgcccg cccgcggccg
       61 gagcctgcgc cacctgcgcc ggccgtaccg ctggcgcctt ggcagccgga ctctgcgctg
      121 gcgtggtgct ctgcgcgaaa agcgtcgcgg acgcggcgag cgaagaaagc aggaaggcga
      181 atttcagctt cttcataaca cccccatgcc tggtagttgt ttctgcgaca gcctgtggtc
      241 gaatcctgca cagtttgtag gggaaaagac atcccatgaa cccgggacgg ttgaccggga
      301 gcaatctccc gggtcttacg gacgaggaga taaacccggt cccccggcca ccgcaccgac
      361 cgccgcctgg gtcgcccggt gcccggccag gaagcgctgc atgttggtcg agccgatatg
      421 gtcgtagccc gcacagtgca gccgctcgtg gtcaacgatg aacttctgcg gcgggaagtc
      481 ggcgctgaac cagatatggc acttgccgcg ggccaggtcg aactcggcgc aggcggccgg
      541 ctccatgccg aagccgacgt agggcttgca ccggtcgcgc atctcggcgt gcggcacgta
      601 gtgctcgacg atctcgagct cgggccagcc ttcgactttt tggtggccaa tgattgagca
      661 tcccgcggcg agcgccgcgg ccacggcaag gagatttcgc atgcgcgcaa ggattgcggc
      721 gcgcccattg cggaatcgca gcccctggtc cggccgttcg gctcacttca cgcataatcg
      781 cgccgcatgc cgtctacacg gatcacctac cagagcggct tcggtaacac cttctccagc
      841 gaagcgttga agggcgcgct ccccgtcggg cgtaactcgc cgcagatccc gcccaagggc
      901 ctgtacgccg aggtgctctc gggcaccgcg ttcaccgcgc cgcgcgccga gaaccagtcg
      961 agctggctct acaagctgcg gccctccgcg atgcacgggc cctacaagcg catcaaggag
     1021 ggcttcctgc gctcagggcc gttcgacgag gtggagaccc cgcccaaccg cctgcgctgg
     1081 gacccgctgc cgatggccac gaagccgacc gatttccttc aggggctcgc gaccatggcg
     1141 ggctccggcg tgccggcgat gcaggtcggc ctcgcggtgc acatctatcg cgccaaccgc
     1201 tcgatgaacg ggcgctactt ctggaacgcg gacggcgagc tcatgttcgt gccgcaggaa
     1261 ggcgcgctgc tgatcttcac ggagctcgga aagctccagg tgaagccagg cgagatcgcc
     1321 gtggtcccgc gcggaatgaa gttccaggtg agcgtcgaag gcccggtgcg cggctacctc
     1381 tgcgagaact acggcgcggc gttccgcctg cccgagctcg gcccgatcgg ctcgcagggg
     1441 ctcgcgcagc cgcgcgactt ccaggcgccg gcggcggcgt acgaagacgg cggaaagtgc
     1501 gaagtcgtgg cgaagttcat gggcgggctg tgggccgccg acttcgcgca ctcgccgctc
     1561 gatgtcgtgg cctggcacgg cgactacgtg ccctacaagt acgacctcgc gcgcttcatg
     1621 acgatcggca cgatcagctt cgaccatccc gatccttcgc tcttcacggt actcacttca
     1681 ccatcggcgc aaccgggtgt ggccaattgc gacttcgtga tcttcccgcc gcgctggctg
     1741 gtggccgagg acacgttccg gccgccgtgg ttccaccgca acgtgatgag cgagctgatg
     1801 ggcctggtgc acggcgtgta cgacgcgaag gccgagggct tcctgccggg cggggtgtcg
     1861 atccacaact gcatgaccgc gcacggcccg gacgtcgcaa cctgggagaa ggcgagcgcg
     1921 gcgaagctcg agccgcagaa aatcaccgac acgctcgcct tcatgtggga gtcgcgctac
     1981 gtgttccggc cgacgaagtt cgcgatgacc gcgcgcgagc tgcagaagaa ctacgacgcg
     2041 gtctggagcg ggttcaggaa gaacctgtaa tgaagtgcga cgagacccac gatccgcagc
     2101 tcacgagctg ggtcgaatcg gcgaatctgc ccggctgcga cttcccgatc cagaacctgc
     2161 cgtt
//
LOCUS       DASUNT010000107         2662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_454316, whole genome shotgun sequence.
ACCESSION   DASUNT010000107 DASUNT010000000
VERSION     DASUNT010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454316"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFX94_02170"
     CDS             complement(<1..183)
                     /locus_tag="VFX94_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEX5767015.1"
                     /translation="MSEPVATDITLHQKSRVMEITFSDGRSFRLPYELLRVYSPSAEV
                     RGHGPGQETLQLSKHEV"
     gene            complement(180..>2662)
                     /locus_tag="VFX94_02175"
     CDS             complement(180..>2662)
                     /locus_tag="VFX94_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004543744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3400 domain-containing protein"
                     /protein_id="HEX5767016.1"
                     /translation="IVEVKNYIEKSALVRLAGLEHLDERYVKAVGYATKSRRSERPRM
                     VLIGDLVGEEVDAVAAAASAVVRIANARGGEGFVAVSEEARKRFWVDRARTAAIARHT
                     NAFKINEDVVIPLEKLGEYTDAVERINIEFSIASKLKLLEELEQYFSRDLRIADGELA
                     RERAGRAQELLRRVHGRWRAIGADLDHYFDRLQSYALRLSWKTEVREELRRIFDGAAF
                     RHVLDEIDNVHKNVLRSRVFIALHMHAGDGNVHTNIPVNSDDYDMLQRAYGAVARLMR
                     LARSLGGAISGEHGIGITKLEFLDPADLEAFRAYKEAVDPAGRFNSGKLVSGGDLRNA
                     YTPSFALIGAESLILEQTDLGRVAGAIKDCLRCGKCKPPCATHVPRANLLYSPRNKIL
                     ATSLLIEAILYEEQTRRGVALAHYDEFGDVADHCTVCHKCKPACPVDIDFGDVSVTMR
                     NYLRRVGKRRFNPGTQASMLFLNLTDPRAVKMARSAMIGWGYRLQRLAHGLLRSISKQ
                     QTARPPLTTGQKPLRAQLIHFVNKPMPGRLPKRTARALLDIEDPKVVPIIRDPAKVSD
                     ESDAVFYFPGCGSERLFSQVGLATQAMLYAVGAQCVLPPGYLCCGYPQTAAGEAEKGE
                     RITMQNRVLFHRLATTLNYLDIRTVIVSCGTCLDQLEQYDFGSIFPGARLLDIHEYLL
                     EKGVKLRDAAGVRYMYHDPCHTPIKTAKPLGVVNGLMGGNVPLSERCCGESGTLAMTR
                     PDVSTQVRFSKEESLKKSMREMKSDTPVKVLTSCPSCLQGLARFENDTGAQADYIVVE
                     LARRMLGEDWLPQFVARANAGGIERVLL"
BASE COUNT          437 a          874 c          881 g          470 t
ORIGIN      
        1 gacctcgtgc ttgctcagct gcaacgtctc ctggcccggc ccgtgaccgc gcacctccgc
       61 cgacggcgag tagacgcgca acagctcgta tggcagccgg aagctgcgcc cgtcggagaa
      121 cgtgatctcc atgacacgcg atttctggtg cagcgtgatg tccgtggcga ccggctcgct
      181 catagcagaa cccgctcgat gccgcccgcg ttcgccctgg cgacgaactg cggcagccag
      241 tcctcgccca gcatgcgacg cgcgagctcg accacaatgt agtcggcctg cgcgccggtg
      301 tcattctcga agcgcgcgag cccctgcagg caggaggggc acgacgtgag gactttgacc
      361 ggcgtgtcgc ttttcatttc gcgcatgctt ttcttcaggc tttcctcttt gctgaaccgt
      421 acctgggtcg acacgtcggg tcgcgtcatc gcaagcgtgc cggactcgcc gcagcagcgc
      481 tccgagagcg gcacattgcc ccccatcaag ccgttcacga caccgagcgg cttcgccgtc
      541 ttgatcggcg tgtggcaggg gtcatggtac atgtaccgaa cgccggcggc gtctctcagc
      601 ttcacgcctt tctcgagcag gtattcatgg atgtcgagca gccgcgcgcc cggaaaaatg
      661 gagccgaagt cgtactgctc gagttggtcg aggcaggtgc cgcacgagac gatcaccgtg
      721 cgaatgtcga ggtagttgag cgtcgtcgca aggcggtgaa agagcacgcg gttctgcatg
      781 gtgatgcgct cgcccttctc ggcctccccg gccgcggttt gcgggtagcc gcagcaaagg
      841 tagcccggcg gcagcacgca ctgcgcgccc accgcgtaga gcatggcctg tgtcgcgagc
      901 cccacctgcg agaacagccg ctccgagcca cagcccggga agtagaacac tgcgtcggat
      961 tcatcgctca ccttcgccgg atcccggatg atcgggacga ccttggggtc ctcgatgtcg
     1021 agcagcgcgc gcgcggtgcg cttcggcagg cgtccaggca tcggcttgtt gacgaaatga
     1081 ataagctgcg cgcgcagcgg cttctggccc gtggtgagcg gcggccgggc tgtctgctgt
     1141 ttcgagatcg accgcaggag cccatgggcg aggcgctgca gccggtaacc ccagccaatc
     1201 attgcgctgc gcgccatctt caccgcgcgc ggatcggtga ggttcaggaa cagcatcgag
     1261 gcctgggtcc cgggattgaa gcgccgcttg cccacgcggc gcagatagtt gcgcatcgtg
     1321 accgagacgt cgccgaaatc gatgtcgact ggacacgcgg gcttgcattt gtggcacacg
     1381 gtgcagtggt cggcgacgtc gccgaactcg tcgtagtgtg cgagcgcgac gccgcgccgc
     1441 gtctgctcct cgtagaggat cgcctcgatg agaagcgagg tggcgaggat cttgttgcgc
     1501 ggagagtaca gcaggttcgc gcgcggtacg tgggtcgcgc acgggggctt gcatttgccg
     1561 cagcgcaggc agtccttgat cgcgccggcg acgcgcccga ggtcggtctg ctccaggatc
     1621 agcgattcgg cgccgatcag cgcgaagctc ggcgtatagg cgttgcgcag gtcaccgccc
     1681 gacacgagct tgccggaatt gaaccgcccg gcgggatcga cggcttcctt gtaggcgcgg
     1741 aacgcctcca ggtcggccgg atcgagaaat tcgagcttgg taatgccaat gccgtgctcg
     1801 cccgagatcg ctccgccaag cgagcgcgcg agccgcatga gccgtgccac ggctccgtag
     1861 gcccgctgca gcatgtcgta gtcgtcggag ttgaccggga tattcgtgtg cacgttgccg
     1921 tcgccggcat gcatgtgcag cgcgatgaag acgcggctgc gaagcacgtt tttatggaca
     1981 ttgtcgatct cgtcgagaac atggcggaag gcggcgccgt cgaaaatgcg gcgcagctcc
     2041 tcgcgcacct ccgtcttcca cgagaggcgc agcgcgtagg actgcaggcg atcgaaataa
     2101 tggtccaggt cggcgccgat cgcccgccag cggccgtgca cccggcgcag cagctcctgc
     2161 gcccgccccg cgcgctcgcg ggcaagctcg ccgtcggcaa tccgcaggtc gcgggagaag
     2221 tactgctcga gctcctccag cagcttgagc ttgctcgcga tggagaactc gatattgatg
     2281 cgctccactg cgtcggtgta ctcgcccagc ttctccagcg ggatcaccac gtcctcgttg
     2341 atcttgaagg cattggtgtg ccgcgcgatc gccgcggtgc gcgcgcggtc gacccagaag
     2401 cgcttgcgcg cctcctccga taccgcgacg aagccctcgc cgccgcgcgc gttggcgata
     2461 cgtaccaccg cggaggccgc cgcggccacc gcatcgactt cctcgccgac gagatcgccg
     2521 atcagcacca tgcgcgggcg ctcgctgcga cgcgacttgg tggcgtagcc aaccgccttc
     2581 acgtagcgct catccagatg ctccaggccc gcgaggcgca ccagagcgga tttttcaatg
     2641 tagttcttta cttccacgat gg
//
LOCUS       DASUNT010000108         2544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1068130, whole genome shotgun sequence.
ACCESSION   DASUNT010000108 DASUNT010000000
VERSION     DASUNT010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068130"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFX94_02180"
     CDS             <1..219
                     /locus_tag="VFX94_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5767017.1"
                     /translation="GEVMTANPRTIEPDRPFGHALHLMYEGEFRHVPVVENGRPVGMV
                     SARDALEPDLRQFIADLEKRVHIGEILG"
     gene            complement(231..2201)
                     /locus_tag="VFX94_02185"
     CDS             complement(231..2201)
                     /locus_tag="VFX94_02185"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEX5767018.1"
                     /translation="MSIPPILWQPSERAIEEAQVTQFARQVIRKHRLDLNSYPDFHRW
                     TVENPEAFWSEVWDSCGVIASRKGNTVLVDGGKMPGARWFPEARLNFAENLLRRGDRG
                     DALVFWDESGAKRRLSYSDLTSEVSRAAQALQALGLRAGDRASAFIPNLPEASMLALA
                     ALSQGIVWSSCSPDFGVDGVLERFGQIEPKVLFCADGYRYNGQTHDSLGRVREIAERL
                     PTLRKVVVVPYLDPDVDVSDVPKAVRLDEWLRRYQPADIAYAQLPFNHPVFVLFTSGT
                     TGKPKCIVHGAGGSLLQCLKTFKLHFDVRPGERFFYFCTTNWVVWNILFAGLCTEAAL
                     MLYDGSPFARNARVLFDYAEKERFTHFGTSAKFIDALSKRGLRPIDTHDLSCLRMIVS
                     TGSPLAPESFDYVYSSIKKDVCLSSISGGTDIMGAFVDASAVLPVYRGELQCRSLGMA
                     VEVFDEEGNAVTNQKGELVCTRPFPSMPLGFWNDRGHERYRAAYFAKYPNVWTHGDWS
                     ELTERGTMLVYGRSDATLNPGGVRIGTAEIYSVVERIDEVEESVAIGQLWPPDNPTDT
                     RIVLFVKLRPGYMLEDALEERMRHEIRRHASPRHMPARIVQVSDIPRTKNGKVVELAV
                     KAAVHGMPVAHTEALANPEALELFRNLEELAT"
     gene            2238..>2544
                     /locus_tag="VFX94_02190"
     CDS             2238..>2544
                     /locus_tag="VFX94_02190"
                     /inference="COORDINATES: protein motif:HMM:NF026200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-pyrroline-5-carboxylate dehydrogenase"
                     /protein_id="HEX5767019.1"
                     /translation="MREAIRSQHRAPEADAVARLRRLQPDDATSVRITETALRLTRRV
                     RDTAPGALSAESFLRSYGLTTREGVALMCVAEALLRIPDADTQDALIRDKLAFGNWSS
                     "
BASE COUNT          441 a          841 c          841 g          421 t
ORIGIN      
        1 ggcgaagtca tgaccgccaa tccgcgcacc atcgagccgg accgcccgtt cggccacgcg
       61 cttcatctca tgtacgaggg cgaattccgc catgtgccgg tggtggagaa cggcaggcca
      121 gtcggcatgg tgtcggcgcg cgatgcgctc gagccggacc tgcgccagtt catcgccgac
      181 ctggaaaagc gcgtccacat cggcgaaatc ctcggctaac ccaaaaaatc ctaagttgct
      241 agctcttcga gattcctgaa aagctcgagc gcctccgggt tggcgagggc ctcggtatgt
      301 gcgaccggca tgccgtgcac ggccgccttg acggcgagct cgaccacctt gccgttcttc
      361 gtgcgcggga tgtcgctcac ctgcacgatg cgcgccggca tgtggcgcgg gcttgcgtgg
      421 cggcggatct cgtggcgcat gcgctcttcc agcgcgtcct ccagcatgta gccgggccgc
      481 agcttcacga acagcacgat gcgcgtgtcg gtcggattgt cgggcggcca gagctgtccg
      541 atggcgaccg attcctcgac ctcgtcgatg cgctcgacca cgctgtagat ctcggccgtg
      601 ccgatgcgca cgccgcccgg gttcagtgtc gcgtccgagc gtccgtacac cagcatcgtg
      661 ccgcgctcgg tgagctcgct ccagtcgccg tgcgtccaca cgttcggata cttggcgaag
      721 taggccgccc ggtagcgctc gtggccgcgg tcgttccaga agccgagcgg catggaaggg
      781 aaaggcctcg tgcagacgag ctcgcccttc tggttcgtca ccgcgtttcc ctcttcatcg
      841 aatacctcga ccgccatgcc gagcgaacgg cactggagct cgcctcggta caccggaagc
      901 accgcgctcg catcgacgaa ggcgcccatg atgtccgtgc cgccggaaat cgatgaaagg
      961 caaacgtcct tcttgatcga cgagtacacg tagtcgaagc tctccggcgc aagcggcgag
     1021 ccggtcgaga cgatcatcct caggcaggac agatcgtgcg tatcgatcgg ccgcagcccg
     1081 cgcttggaaa gcgcgtcgat gaatttcgcc gaggtgccga aatgggtaaa gcgctccttc
     1141 tcggcgtagt cgaagagcac cctcgcattg cgcgcgaacg gcgagccgtc gtagagcatg
     1201 agggccgcct cggtgcatag cccggcgaag aggatgttcc acaccaccca gttggtcgtg
     1261 cagaaataga agaagcgctc gcccgggcgc acgtcgaagt gcagcttgaa cgtcttcagg
     1321 cattgaagca gcgagccgcc cgcgccgtgc acgatgcatt tcggcttgcc cgtcgtgccg
     1381 gaagtaaata gcacgaacac cgggtggttg aagggcagct gcgcgtaggc gatgtcggcg
     1441 ggctgatagc gccgcagcca ctcgtccagc cgcactgcct tcggcacgtc cgaaacatcg
     1501 acgtcggggt cgaggtacgg aacgacaacc accttgcgga gcgtcggcag gcgctcggcg
     1561 atctcgcgca cgcgcccgag cgagtcgtgc gtctgcccgt tgtagcggta gccgtcggcg
     1621 cagaacagca ccttgggctc gatctggccg aagcgctcga gcacgccgtc cacgccgaag
     1681 tccggcgagc acgaggacca gacgatgcct tgcgacagcg cggcgagcgc cagcatgctc
     1741 gcttcgggaa ggttcggaat gaaggccgag gcccggtcgc cggcccgcag gccgagcgcc
     1801 tgcagcgcct gcgccgcgcg cgacacctcg ctcgtcaggt ctgaatagga aagccgccgc
     1861 tttgcccccg actcgtccca gaacacgagc gcgtcgccgc gatcgccgcg tcgcagcagg
     1921 ttctcggcga agttgagccg cgcctcgggg aaccagcggg cgccgggcat cttgccgccg
     1981 tccaccagca cggtattgcc cttgcgcgag gcaatcacgc cgcacgagtc ccacacctcg
     2041 ctccagaaag cctcggggtt ctccaccgtc cagcggtgga aatcgggata gctgttcagg
     2101 tcaaggcgat gcttgcggat cacctggcgc gcgaactgcg tgacttgcgc ctcttcgatc
     2161 gcgcgctccg agggctgcca gaggatcggc gggatggaca tatcggcagt acgattttgc
     2221 gcggattata gtgaggcatg cgagaagcga ttcgcagcca gcaccgcgct ccggaggccg
     2281 acgcggtggc gcggctgcgc cggctgcaac cggatgatgc gacctcggtt cggattacgg
     2341 aaaccgcgct tcggctgaca cggcgcgtgc gggacaccgc gcccggtgcg ttatcggccg
     2401 agtccttcct gcgcagctat gggctcacca cgcgcgaggg cgtcgcgctc atgtgcgtgg
     2461 cggaagcgct gttgcgtatt cccgacgccg atacgcagga cgcgctgatc cgcgacaagc
     2521 tcgccttcgg caactggagc tccg
//
LOCUS       DASUNT010000109         3321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_454555, whole genome shotgun sequence.
ACCESSION   DASUNT010000109 DASUNT010000000
VERSION     DASUNT010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454555"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /gene="otnK"
                     /locus_tag="VFX94_02195"
     CDS             <1..1147
                     /gene="otnK"
                     /locus_tag="VFX94_02195"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HEX5767020.1"
                     /translation="VTLVREGLSVIQVNGVPDRSLSVPPADCVVVALKSRTNPTAEAV
                     AWSLASLDWLRAHKAERIYFKYCSTFDSTARGNIGPVADALLDALGERFTVATPAYPR
                     NGRTVFKGNLFVGDVPLAESGMKDHPLTPMRDSNLVRVLAAQTPHEVGLVPSEVMDAG
                     PLAIRKRFEELRKSARYAVLDAVRDEHLVAAGEACRDMTLTTGGAGLAMGIARALGKR
                     GPAAQALPRLGGPAVVLAGSCSQATLGQIAAWTAPALKLDPHKLDAAAAVDWAARNLE
                     STPLIYASAPAREIALGRGEAIEQAFRDMARALAAKGVRKFVVAGGETSGAVVEALGV
                     RALAIGPEIDPGVPWTAAVGGEPYLLALKSGNFGSRDFFRKALEMVA"
     gene            1144..1767
                     /gene="otnC"
                     /locus_tag="VFX94_02200"
     CDS             1144..1767
                     /gene="otnC"
                     /locus_tag="VFX94_02200"
                     /EC_number="4.1.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate 4-phosphate decarboxylase"
                     /protein_id="HEX5767021.1"
                     /translation="MTENELREALVLHGRSLYERGYAHGSSGNLSVRLPDGLLITPTN
                     SCLGRLDPARISKVNRNGEHLSGDAPSKEAFLHLAMYQERPSAQGIVHLHCTHCVAVS
                     CLQGFTALPPITAYYVMRIRELALIPYYRPGDRALAEAVREEAKKHHAVLLANHGPVV
                     AGKSLEDAVVNAEELEETAKLYLLLEGRRTRFLDATQIADLEKHFPS"
     gene            1777..2061
                     /locus_tag="VFX94_02205"
     CDS             1777..2061
                     /locus_tag="VFX94_02205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX5767022.1"
                     /translation="MYVVLVDFKVKPERLKDFMPLMLENARASRETEPGCRQFDVCVD
                     PADRTKVFLYEVYDNRKAFDAHLATLHFKQFDPAVAPMVASKAVRMLERV"
     gene            2083..3033
                     /locus_tag="VFX94_02210"
     CDS             2083..3033
                     /locus_tag="VFX94_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5767023.1"
                     /translation="MKRLLIVLLWALAVPALAQPYPSKPVRLIVPYPPGGGNDTLGRL
                     FAAKLGERTGQQFVVENRPGAGAMIGTEAAAKSAPDGYTILLSSIATHALSPNLYSRV
                     PYDPVKDFAPITLLGIAPTVLVVNAELPAKNLRELIDLSRKQQLAYASGGNGTPPHIN
                     AEVFKALAGVDLLHVAYKGGGPALVDLMAGRVQLMLDTAASAMPHVRSGKLRALALSA
                     PKRSADLPELPTFAEAGLPQYDTNAWYSMHAPAGTPPDIVRRLNAELVAILRDPDIVA
                     RFKQLATDPVGNSPEEFAAFVKSELEKYARVIKSAGIKLD"
     gene            3033..>3321
                     /locus_tag="VFX94_02215"
     CDS             3033..>3321
                     /locus_tag="VFX94_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX5767024.1"
                     /translation="MKLLVLEGDGIGPEITAATLGVLESASRKFGLGLEYEKRDIGFA
                     ALKKHHTTLPDGVLERTRELDGVILGPISHLDYPPADEGGVNVSAAFRVKLD"
BASE COUNT          548 a         1146 c         1090 g          537 t
ORIGIN      
        1 cgtgacgctg gtgcgcgaag gcttgtcggt gatccaggtg aacggcgtgc cggaccgctc
       61 gctctccgtg ccgccggccg attgcgtcgt cgtcgccctc aagtcgcgca ccaacccgac
      121 cgccgaagcg gtcgcctggt ctctcgcttc tctcgactgg ctgcgagccc ataaggccga
      181 gcgcatttac ttcaagtact gctcgacctt cgactcgacc gcgcgcggca acatcggtcc
      241 ggtagccgat gcgctgctcg acgcgctcgg cgagcgcttc accgtcgcca cgcccgccta
      301 cccgcgcaat ggccgcacgg tcttcaaggg aaacctcttc gtcggcgacg tgcccctcgc
      361 cgaatcgggg atgaaagatc atccgcttac gccaatgcgc gactcgaacc tggtgcgtgt
      421 gctcgccgcg cagactccac acgaggtggg cctggttcct tctgaagtca tggacgcagg
      481 gccgctcgct atccggaaaa gattcgaaga gctccggaaa agcgcccgtt acgccgtgct
      541 cgacgccgtt cgcgacgagc accttgtcgc cgccggcgag gcatgccggg acatgacgct
      601 cacgaccggc ggcgcggggc ttgccatggg catcgcacgc gcgctcggaa agcgaggccc
      661 ggccgcgcaa gccctgccgc ggctcggcgg tccggcagtc gtcctcgccg gcagctgctc
      721 gcaggcgacg cttggacaga tcgcggcctg gacggcgccc gccctcaagc tcgatccgca
      781 taaattggat gccgcggccg ccgtggactg ggcggcgcgc aacctggaat ccacgccact
      841 catctacgcg agcgcgcctg ccagggagat cgcgctgggg cgcggcgaag ccatcgagca
      901 ggcgtttcgc gacatggcgc gcgcgctcgc ggcgaaaggc gtgcgcaagt tcgtggtcgc
      961 gggcggcgag acctccggcg ccgtggtcga ggcgcttggg gtgcgagcgc ttgccattgg
     1021 cccggagatc gacccgggcg tgccctggac cgccgcggtc ggcggcgagc catacctcct
     1081 cgcgctcaag tcgggtaact tcggcagccg ggatttcttc cgcaaggcgc tggagatggt
     1141 ggcgtgaccg agaacgagct gcgcgaagcg cttgtactgc acggccgctc gctctatgag
     1201 cgcggctacg cgcacgggtc ctcggggaac ctgagcgtgc gcctgcccga cggcctgctc
     1261 atcacgccga cgaactcgtg cctcggacgc ctggatccgg cgcgcatttc caaagtgaat
     1321 aggaacggtg aacacttgtc gggtgacgcg ccctccaagg aagcgtttct gcacctggcg
     1381 atgtaccagg aacgcccgtc cgcgcaaggc atcgttcacc tgcactgcac gcactgcgtc
     1441 gcggtgtctt gcctgcaagg cttcacggca ctgccgccga tcaccgcgta ttacgtcatg
     1501 cgcatccgcg agctggcgct cattccgtac taccggccgg gcgaccgcgc gctcgccgaa
     1561 gcggtgcgcg aggaggcgaa gaagcaccac gccgtgctgc tcgcgaacca cggcccggtc
     1621 gtcgccggga aatcgctcga ggatgcggtg gtcaacgccg aggagctgga agagacggcc
     1681 aagctctatt tactcctcga agggcggcgc acccggttcc tcgacgcgac gcagatcgcc
     1741 gacctggaaa agcatttccc gagctgaaag gaccgcatgt acgtcgtcct cgtcgacttc
     1801 aaggtcaagc ccgagcgcct gaaggatttc atgccgctca tgctcgagaa tgcgcgcgcc
     1861 tcgcgcgaga ccgagcccgg ctgccggcag ttcgacgtgt gcgtcgatcc tgccgatcgg
     1921 acgaaagtgt tcctctacga ggtgtatgac aaccgcaagg ctttcgatgc gcacctcgcc
     1981 acgctgcatt tcaagcagtt cgatccggcg gtggcgccca tggtcgcgtc gaaggcggtg
     2041 cgcatgctgg agcgggttta agggaggatc cagggagttt tcatgaagag attgctcatc
     2101 gtgctgctat gggcgctcgc cgtgccggcg ctcgcgcaac cctatccctc caagccggtg
     2161 cgcctgatcg ttccctaccc gcccggcggc ggcaacgaca cgctcggtcg cctgttcgcg
     2221 gcgaagctcg gcgagcgcac gggccagcag ttcgtggtcg agaaccggcc cggcgccggg
     2281 gcgatgatcg gcaccgaggc ggcggcgaag tcggcgcccg acggctacac catcctgctc
     2341 agctccatcg cgacgcacgc gctcagcccg aacctctact cgcgcgtgcc ctacgatccg
     2401 gtcaaggatt tcgcgccgat cacgctgctc ggcatcgcgc cgaccgtcct tgtcgtgaat
     2461 gccgagcttc cggcgaagaa tctgcgggag ttgatcgatc tttccaggaa acagcagctc
     2521 gcctacgctt cgggcgggaa tggcacgccg ccgcacatca acgccgaagt gttcaaggcg
     2581 ctcgccggcg tggacctcct gcacgtcgcg tacaagggcg gcgggcccgc gctggtagac
     2641 ctgatggccg gccgcgtgca actgatgctc gataccgccg cctccgccat gccgcacgtg
     2701 cgctcgggca agttgcgcgc gctcgcgctc tccgcgccga agcgctcggc ggacctgccg
     2761 gagttgccga ccttcgcgga ggccggcctg ccgcagtacg acaccaatgc ctggtactcg
     2821 atgcacgccc ccgccggcac gccgcccgac atcgtgcgcc gcctgaatgc cgagctggtc
     2881 gcgatcctca gggatccgga catcgtggcg cgcttcaagc agcttgccac cgatccggtg
     2941 ggcaactcgc ccgaagagtt cgccgccttc gtgaagagcg agctggagaa gtacgcgcgt
     3001 gtcatcaaga gcgccggaat caagctcgac tgatgaagct cctcgtcctc gaaggcgacg
     3061 gcatcgggcc cgagatcacc gccgccacgc ttggcgtcct cgaaagcgcc agccggaagt
     3121 tcgggcttgg gctggaatac gaaaagcgcg atatcggttt cgcggcgctc aagaaacacc
     3181 acaccacgct gcccgacggc gtgctggaac gcacacgcga gctggatggc gtgatcctcg
     3241 ggccgatctc gcaccttgac tatccgccgg ccgacgaggg cggcgtcaac gtgtcggccg
     3301 cgttccgcgt gaagctcgac c
//
LOCUS       DASUNT010000110         4316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_541848, whole genome shotgun sequence.
ACCESSION   DASUNT010000110 DASUNT010000000
VERSION     DASUNT010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541848"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /gene="ftsA"
                     /locus_tag="VFX94_02220"
     CDS             <1..597
                     /gene="ftsA"
                     /locus_tag="VFX94_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX5767025.1"
                     /translation="GGTTDIAVFTHGAIRHTAVVPIAGDQITNDIAMALRTPTADAEA
                     IKIRHGVALRSAADPNAMIEVPGIGERGSRSMSRQTLAEVIEPRVEELYSLVQQVLRE
                     SGFEELLSSGVVLTGGSAVMQGMVELGEEIFHMPVRIGLPRYAGALAEVLRTPRYATA
                     MGLLYEGVAQREQGKLSRQNGSVKAVVGRMREWFQRNF"
     gene            629..1786
                     /gene="ftsZ"
                     /locus_tag="VFX94_02225"
     CDS             629..1786
                     /gene="ftsZ"
                     /locus_tag="VFX94_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX5767026.1"
                     /translation="MFEIIDSEATGPVIKVMGVGGAGGNAVNHMIETGVQGVEFIAVN
                     TDAQVLSRNKARNQLQLGSSGLGAGARPEEARAAALAERERIEEAVAGSHMVFITAGM
                     GGGTGTGAAPVIAEVARGLGILTVAVVTKPFTFEGSKRMKVAEQGIEALQPHVDSLIV
                     ILNDKLEEVLGEDVTQEDAFKAADDVLNNAVGGIAEIINLPGLVNVDFQDVKTVMSEQ
                     GMAMMGSASASGIDRARIAAEQAIACPLLEGVNLAGARGVLVNITASRPSLKLRETKE
                     VMNIIRTFAAEDATIIYGGVYDDSLADSLRVTVVATGLGLGTSARQQPKPQLVVSQKT
                     GTDNAPGPVDYHQLEQVPAVIRRNRASTVEALQQSGVDKYDIPAFLRKQAD"
     gene            1894..3096
                     /locus_tag="VFX94_02230"
     CDS             1894..3096
                     /locus_tag="VFX94_02230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5767027.1"
                     /translation="MAESTPPASGDALFGALFEQSAAAIAVFSIGGRFLRANPAMCRL
                     LGYTEQELMQKTHLDVIHIEDLEAAAVARAQVISGKAKPRLAERRYVHKDGSTVWVQM
                     SGAVVHDESDAPQCTVLVANDISALKQSLRRAKGRFRRMVEMSSDWYWMQDEHFRFVE
                     VAGLDTQDGDTDVVVGRARWEIPGLGALPDKVWENHRARLERHESFSDFVFLRYNKAG
                     ELRYLSVSGEPLFDEKGNFIGYHGVGKDITERARDQKALEDSERRYRTLFEVHPQPMW
                     VVDANTLAFLAVNGAAVRLYGYSKEEFLRMSADQIRPEEDIDDLRRAFADWSNNYSQR
                     LWRHRKKSGEVIPVKVTSFNLEFAGRRARLGVIEDLSERLQAEERAKQSEQRYRSLLE
                     SRNTGSEE"
     gene            3122..4036
                     /gene="lpxC"
                     /locus_tag="VFX94_02235"
     CDS             3122..4036
                     /gene="lpxC"
                     /locus_tag="VFX94_02235"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012763007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX5767028.1"
                     /translation="MVKQRTLKSLIRASGVGLHTGQKVRMTLRPAPADTGVVFRRIDL
                     EPPVDIPARAQLVGEARLASTLIKDGVKIHTVEHLMSALAGLGIDNVFVDLDAPELPI
                     MDGSASPFALLLQQAGLEEQAAPKRFLRVRTTVEVRDGDKWARLEPHSGFRLSFSIDF
                     RHPVIERSTQSVTLDFAETSYLKEIARARTFGFMHEVEDLKDSGLALGGGLDNAVVLD
                     EHGVLNSEGLRFADEFIRHKLLDAVGDLYLLGRPLLGAFTAHKSGHALNNRLARAALA
                     EPGALETVVFERAEEAPAGIARLSAQTG"
     gene            4037..4234
                     /locus_tag="VFX94_02240"
     CDS             4037..4234
                     /locus_tag="VFX94_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767029.1"
                     /translation="MFAVLRIAVVVVAIVLGFAVLAWLLTGDARWRRFAWLVFKYAVF
                     ALAVILLLFAGEALLGEVLLH"
BASE COUNT          752 a         1404 c         1495 g          665 t
ORIGIN      
        1 ggcggcacga ccgacatcgc ggtgttcacg cacggcgcca tccggcatac ggcggtggtg
       61 ccgattgccg gcgaccagat caccaacgac atcgcgatgg cgctgcgcac gccgaccgcg
      121 gacgcagagg ccatcaagat ccgccacggc gtggcgctgc gctcggcggc ggacccgaac
      181 gcgatgatcg aggtgccggg catcggcgag cggggctcgc gctcgatgtc gcgccagacc
      241 cttgccgagg tgatcgagcc gcgcgtcgag gagctctatt cgctggtgca gcaggtgctg
      301 cgcgaatcgg gcttcgagga gctgctgtcg agcggcgtgg tgctgaccgg cggctcggcg
      361 gtcatgcagg gcatggttga gctcggcgag gagatcttcc acatgccggt gcgcatcggg
      421 cttccgcgct acgccggcgc gctggcggaa gtgctgcgca caccgcgcta cgccaccgcg
      481 atgggcctgc tttacgaggg cgtagcgcaa agggagcagg gcaagctttc gcgccagaac
      541 ggctcggtga aagccgttgt ggggcgcatg cgggagtggt tccagcgtaa tttctgatct
      601 gtaacgacac ggcaaggaga aaacaacaat gtttgagatc atcgacagtg aggcaacagg
      661 tccggtcatc aaggtgatgg gggtgggcgg cgccggcggc aacgccgtca accacatgat
      721 cgaaaccggc gtgcagggcg tggagttcat cgccgtcaat accgacgccc aggtgctttc
      781 gcgcaacaag gcgcgcaacc agctgcagct cggctcgagc gggctcggcg ccggggcgag
      841 gcccgaggag gcgcgcgccg cggcgctcgc cgagcgcgag cgcatcgaag aagccgtcgc
      901 cggctcgcac atggtattca tcaccgcggg catgggcggc ggtaccggca ccggcgccgc
      961 gccggtcatc gccgaagtcg cgcgcggcct gggcattctc accgtcgccg tggtcaccaa
     1021 gccattcacc ttcgaggggt ccaagcgcat gaaggtcgcc gagcagggca tcgaggcgct
     1081 ccagccgcac gtcgactcgc tcatcgtcat cctgaacgac aagctggagg aagtgctcgg
     1141 cgaggacgtg acccaggagg acgcgttcaa ggcggccgac gacgtgctga acaacgccgt
     1201 cggcggcatc gccgaaatca tcaacctgcc cggcctggtg aacgtcgact tccaggacgt
     1261 gaagacggtc atgtcggagc agggcatggc gatgatggga tcggcatcgg cgagcggcat
     1321 cgaccgcgcg cgcatcgcgg ccgagcaggc gatcgcgtgt ccgctgctcg aaggcgtgaa
     1381 ccttgccggc gcgcgcggcg tgctggtcaa catcacggcg tcgcggccga gcctcaagct
     1441 gcgcgagacc aaggaagtga tgaacatcat ccgcaccttc gccgccgagg acgcgaccat
     1501 catctacggc ggggtctacg acgattcgct ggccgacagc ctgcgcgtca cggtggtggc
     1561 caccggcctg ggcctgggca cctcggcgcg ccagcagccc aagccgcaac tcgtggtgtc
     1621 gcagaaaacc ggcaccgaca acgccccggg cccggtggac taccaccagc tcgagcaggt
     1681 gcccgcggtg atccggcgta accgcgcctc gacggtagag gcgctccagc agagcggcgt
     1741 ggacaagtac gacatcccgg ctttccttag aaagcaggcg gactaagggg aaaaacgagc
     1801 gaagtagcgc gcttggcaaa ggatggcaat aagcggggac cccaccttgc cgggggtgcc
     1861 ctgcggctgc cttcgtgtca tgatgaccgg gccatggctg aatcgacccc cccagcctcc
     1921 ggcgatgcgc tgttcggcgc gctcttcgag cagtcggcag cggcgatcgc ggtgttctcg
     1981 atcggcgggc gcttcctgcg cgcgaacccg gcgatgtgcc ggctgctcgg ctataccgag
     2041 caggagctca tgcaaaagac gcatctcgac gtgatccaca tcgaggacct cgaggcggcg
     2101 gccgtggcgc gggctcaggt gatctccgga aaggcgaagc cgcggctcgc cgagcgacgc
     2161 tacgtccaca aggacggctc caccgtctgg gtgcagatga gcggtgccgt ggtgcacgac
     2221 gagtcggacg ctccgcagtg caccgtgctc gtggcaaacg acatctcggc gctgaagcaa
     2281 tcgctgcgcc gggcgaaggg ccgcttccgg cgcatggtgg agatgagctc cgactggtac
     2341 tggatgcagg atgagcattt caggttcgtc gaggtcgcgg ggctcgacac ccaggacggc
     2401 gacaccgacg tcgtggtcgg aagggcgcgc tgggaaatcc cgggcctcgg cgccctgccg
     2461 gacaaggtct gggagaacca tcgcgccagg ctggagcgcc acgagtcgtt ctccgatttc
     2521 gtcttcctgc gctacaacaa ggcgggcgag ctgcgttatc tctcggtaag cggggagccg
     2581 ctcttcgacg agaagggcaa cttcatcggc taccacgggg tcggcaagga catcaccgag
     2641 cgcgcacgcg atcaaaaggc gctcgaggac agcgagcggc gctaccggac gctgttcgaa
     2701 gtccatccgc agcccatgtg ggtggtggat gccaacacgc tggcgttcct cgccgtgaac
     2761 ggcgcggccg tgcgtcttta cggatactcc aaggaagagt tcctgcgcat gagcgccgac
     2821 cagatccggc ccgaagagga tatcgacgat ctgcgccgcg cctttgccga ctggagcaac
     2881 aactacagcc agcggctgtg gcgccatcgc aagaaaagcg gcgaagtgat tccggtcaag
     2941 gtcacttcgt tcaacctcga gttcgccgga cgccgcgcgc gcctcggcgt catcgaggac
     3001 ctgagcgagc gcctccaggc cgaagagcgc gccaagcaga gcgagcagcg ttatcggtcg
     3061 ctgctcgaaa gccggaacac gggctccgag gagtagaatg ctgttgcccg tgaacgccta
     3121 gatggtcaag cagcgcaccc tgaaatcgct catccgcgcc tccggcgtcg ggctgcatac
     3181 cgggcaaaaa gtgcgcatga cgctgcgccc ggcgcccgcc gacaccggcg tcgtgttccg
     3241 ccgcatcgac ctcgagccgc cggtcgacat tccagcgcgc gcgcagctcg tgggcgaagc
     3301 gcgcctcgcc tccacgctga tcaaggacgg cgtgaagatc cacaccgtcg agcacctgat
     3361 gtctgcgctg gcgggactgg gcatcgacaa cgtgttcgtc gacctggacg cgcccgagct
     3421 gccgatcatg gacggcagcg cctccccctt cgcgctgctt ctgcagcagg cggggctgga
     3481 agagcaggcc gccccgaaaa gattcctgag agtcagaaca accgtcgaag tgcgcgatgg
     3541 cgacaagtgg gcgcgcctgg agccgcattc cggcttccgg ctctcgttct cgatcgactt
     3601 ccgccacccg gtgatcgagc gcagcacgca gtcggtcacc ctggacttcg ccgagacctc
     3661 gtacctcaag gaaatcgcgc gcgcgcgcac gttcggcttc atgcacgagg tggaggacct
     3721 caaggacagc ggccttgcgc tcggcggcgg cctcgacaac gccgtggtgc tcgacgagca
     3781 cggcgtgctg aacagcgagg gcctgcgctt cgccgacgaa ttcatccgcc acaagctgct
     3841 cgacgcggtc ggcgacctgt atctgctcgg ccggccgctc ctcggcgcct ttaccgcgca
     3901 caagtcgggg catgcgctca acaaccggct ggcgcgcgca gcgctggccg agccgggagc
     3961 cctcgagacc gtggtgttcg agcgcgccga ggaagcgccc gcgggcatcg ctcgcctttc
     4021 cgcccaaacc ggctaggtgt tcgcggtcct gcggatcgcg gtcgtcgtgg tcgcgatcgt
     4081 gctcgggttc gcggtgctcg cgtggctgct gaccggagat gcacgctggc ggcgctttgc
     4141 gtggctcgtt ttcaagtacg cggttttcgc gctggcggtg atcctgctcc tctttgccgg
     4201 cgaggctctg cttggcgagg tcctgcttca ctaggccttc agcgcgcggc cgcggcgctg
     4261 gagcagcttc cctagtgcat cgcgcgcggg cgaggtcttc atcgagtcgt aaagcc
//
LOCUS       DASUNT010000111         2912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_717418, whole genome shotgun sequence.
ACCESSION   DASUNT010000111 DASUNT010000000
VERSION     DASUNT010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717418"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFX94_02245"
     CDS             <1..222
                     /locus_tag="VFX94_02245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5767030.1"
                     /translation="GRTLVLDHGHGMLTLYSHLSAVHRAVGDAVKAGATIGEVGMTGR
                     ATGPHVHFSVYLNAASVDPAIFLARAAPK"
     gene            complement(224..688)
                     /locus_tag="VFX94_02250"
     CDS             complement(224..688)
                     /locus_tag="VFX94_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX5767031.1"
                     /translation="MKVLLACSLLAVSLSAAAQQAQKSDPSIARGRYVIAIAGCNDCH
                     TPHYPQTGGKVAESEWLTGDALGWRGPWGTTYATNLRLYMQEITEEQWVKKAQRLNAR
                     PPMPWFNLHHMSKADLRAIYRYVQHLGPAGKPAPAYVPPQQKPAGPYVQFPE"
     gene            complement(685..1116)
                     /locus_tag="VFX94_02255"
     CDS             complement(685..1116)
                     /locus_tag="VFX94_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketosteroid isomerase"
                     /protein_id="HEX5767032.1"
                     /translation="MTPQQMDEIINQHFRYEASDDVAGVVGSLADRVQHEVVPSPMGL
                     QTDRNKIASFYTMLFADLKGESVTPLRRLYGKDFVVDESLWHGRIENGRIFLCEGRSG
                     QVSFRLLHVFELDGEKIKREQVWCDLAAVQKQLGVLEKEVA"
     gene            1186..1866
                     /locus_tag="VFX94_02260"
     CDS             1186..1866
                     /locus_tag="VFX94_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5767033.1"
                     /translation="MYREIAKRDLRANQKERTREALVAAAKELARAGAAPTVAEAAEH
                     ARVSRATAYRYFPTQESLLIELSDVTPVVAPVEEMLASLGGDDSEARLAKLLDTFHPI
                     MVAEEVPLRTALRVYLDTWLEARRRGEAAPKVREGRRMRWLDTALEPARRQLPKAEWR
                     RLRAALALTLGIDVLVVMKDVAQLDDEEALSVLRWAALTLLRASLSRAPAASRRASDR
                     SRAARSAR"
     gene            complement(1799..2773)
                     /locus_tag="VFX94_02265"
     CDS             complement(1799..2773)
                     /locus_tag="VFX94_02265"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5767034.1"
                     /translation="MRTVLAALALLVASAAHAQPLKKVVLFGQPSVNNDAIWMALEYG
                     YFKEQGLDVEYRVFPSGTTAFQTFRTGQGDIVMTGDLPSVQYFFNNPGQYQTFAAIER
                     DAKGYVIIARKDIRKAQDLAGKTVATRVGSTGSWFISEYLTKNGVDPKSVPVKNLDTQ
                     LLPAALCKGDISAFFIWQPIGSRTLEICPNDAHQLADASGYIQGYLVAGARPAWLNSP
                     EGADAVTRFLRAIIKGRAKAEADFKAVAAFAAKTYSLSEKATRDQWETNTRPIALDKV
                     YYDDFCSLSKWARGAGLTTEPVDFTKLTWPNGLRAIDPKLAETPPAPC"
BASE COUNT          453 a          980 c          972 g          507 t
ORIGIN      
        1 ggccgcacgc tggtcctcga ccacggccac gggatgctca cgctctactc ccacctgagc
       61 gccgtgcatc gcgcggtcgg cgacgcggtc aaggcaggcg cgaccatcgg cgaggtcggc
      121 atgaccggcc gcgccaccgg cccgcacgtg cacttctcgg tctacctcaa cgcggcgtcg
      181 gtagaccccg cgatcttcct ggcgcgcgcc gcgcctaagt gagctactcg ggaaactgca
      241 cgtagggtcc cgccggtttc tgttgcggcg gcacgtacgc cggggccggc ttcccggcgg
      301 gccccaggtg ctgcacgtag cggtagatcg cgcgcaggtc cgccttgctc atgtgatgca
      361 ggttgaacca gggcatcggc ggacgcgcgt tcagcctctg cgccttcttt acccactgct
      421 cctcggtgat ctcctgcatg tagaggcgca ggttggtcgc gtaggtcgtg ccccaggggc
      481 cgcgccagcc gagcgcatcg ccggtaagcc attcgctttc ggccaccttg ccgccggtct
      541 gcggatagtg cggtgtatgg cagtcgttgc acccggcgat cgcgatgaca tagcggccgc
      601 gcgcgatgct cgggtcggac ttctgtgcct gctgggcggc tgccgacagg ctcaccgcga
      661 gcaacgagca agcaaggagg actttcatgc aacctccttt tccagcacgc cgagctgctt
      721 ctgcaccgcc gcgaggtcgc accatacctg ctcgcgtttg atcttttcgc cgtccagctc
      781 gaacacgtgc agcaggcgaa agctcacctg cccgctcctg ccctcgcaca ggaagatgcg
      841 gccgttctcg atgcggccgt gccagagcga ctcgtcgacc acgaagtcct tgccgtacag
      901 gcggcgcagc ggcgtcacgc tctcaccctt gaggtcggcg aacagcatgg tgtagaagct
      961 cgcgatcttg ttgcggtcgg tctgcaggcc catcggcgaa ggcaccacct cgtgctggac
     1021 ccggtcggcg aggctgccga ccacccccgc cacgtcgtcg ctggcttcat agcggaagtg
     1081 ctggttgatg atctcgtcca tctgctgcgg cgtcattcag cggctccttg cgttgttgat
     1141 gagacatgtg tatcgtgaag atacgtcaat ctcttgtcaa gatatatgta ccgcgaaata
     1201 gcgaaacgcg acctgcgcgc gaaccagaag gagcgcaccc gcgaggcgct cgtggcggcg
     1261 gcgaaggagc tggcgcgcgc cggcgcggcg cccaccgtag ccgaggcggc cgagcacgcg
     1321 cgcgtttcgc gcgccaccgc gtaccgctat ttccccaccc aggaatccct cctcatcgag
     1381 ctgagcgacg tgacgccggt ggtcgcgccg gtcgaggaga tgctcgccag cctgggcggc
     1441 gacgattccg aagcacgcct ggcgaagctc ctcgacacgt tccacccgat catggtcgcc
     1501 gaggaagtgc cactgcgcac ggcgctgcgc gtctacctcg acacctggct cgaggcgcgc
     1561 cgccgcggcg aagcggcgcc caaagtgcgc gagggccggc gcatgcgctg gctcgatacg
     1621 gcactcgagc ccgcccgccg gcagcttccg aaagcggagt ggcgccgcct gcgcgccgcg
     1681 cttgctctca ccctgggcat cgacgtgctc gtcgtcatga aggacgtcgc gcagctcgat
     1741 gacgaggagg cgctctcggt cctgcgctgg gcggcgctaa cgctgcttcg cgcgagcctc
     1801 agcagggcgc cggcggcgtc tcggcgagct tcggatcgat cgcgcgcagc ccgttcggcc
     1861 aggtgagctt cgtgaagtcc accggctcgg tcgtcagccc ggcgccccgc gcccacttgg
     1921 acaggctgca gaagtcgtcg tagtagacct tgtcgagcgc gatcgggcgg gtatttgtct
     1981 cccactggtc gcgcgtcgct ttctcgctca ggctgtaggt cttcgcggcg aaagccgcca
     2041 ctgccttgaa atccgcttcg gccttggcgc gccctttgat gatggcgcgc aggaagcgcg
     2101 tcaccgcgtc cgcgccttcg ggagagttca gccaggcggg ccgcgccccg gcgacgaggt
     2161 agccctggat gtagcccgag gcgtcggcga gctggtgcgc gtcgttcggg cagatctcga
     2221 gcgtgcgcga gccgatcggc tgccagatga agaacgccga aatgtcccct ttgcagagcg
     2281 ccgccggcag cagctgcgtg tcgaggttct tcaccggcac gctcttcggg tcgacgccgt
     2341 tcttcgtcag gtactcggag atgaaccacg acccggtcga gccgacgcgc gtcgcgaccg
     2401 tcttgccggc gaggtcctgc gccttgcgga tgtccttgcg cgcgatgatg acgtagccct
     2461 tcgcgtcgcg ctcgatcgcg gcgaaggtct ggtactggcc cgggttattg aagaagtact
     2521 gcacgctcgg caggtcgccg gtcatgacga tgtcgccctg gccggtgcgg aacgtctgga
     2581 acgcggtggt gccggacggg aatacgcggt actccacatc cagcccctgc tccttgaagt
     2641 agccgtactc gagcgccatc cagatggcgt cgttgttgac cgacggctgg ccgaacagga
     2701 ccactttctt cagcggctgc gcgtgcgcag ccgacgcgac gagcagcgcg agcgcggcaa
     2761 gaacggttct catgcatcct cctctcaggt gagggaaata tacgacatgt agcgcgacag
     2821 catgcggtcg accgcaatgc ggaacagccg gtcggaaatg aagccgagca cgcagatcgt
     2881 gagcagcgcg acgaagatgc ggttggtctg ca
//
LOCUS       DASUNT010000112         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1156273, whole genome shotgun sequence.
ACCESSION   DASUNT010000112 DASUNT010000000
VERSION     DASUNT010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156273"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..920)
                     /locus_tag="VFX94_02270"
     CDS             complement(153..920)
                     /locus_tag="VFX94_02270"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HEX5767035.1"
                     /translation="MQARIVQTARGAQWLLEGWRLFRAAPLGWLAMVFAYWLLMTLVS
                     VVPLVGVAAASIMVPAFSVGFMAAARAAARKGPVELVLLFEGFRNHLRSQLILGVGYF
                     ACLALVLAATALADGGALAGWMLSARRPDEEVLHSDAFLAALGAAAALYTPVLMMFWF
                     APPLAAWHGTAPAKALFFSFFACLMNWRAFLAYGAVTAVVALALPLAVVSVVALASLG
                     VPAMSLVFPLLIVLLPTLFASFYASYRDVFRPDPGTS"
     gene            complement(920..1861)
                     /locus_tag="VFX94_02275"
     CDS             complement(920..1861)
                     /locus_tag="VFX94_02275"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX5767036.1"
                     /translation="MSVYTPVSEAELHEWLRNYSVGRLVACEPIEAGIENTNYFVTTT
                     QGRYVLTLFERLPAEELPFYLDLMAHLARHGIPCPAPIADLSDQYLGRLNGKPAALVT
                     RLPGRSLEGPGARHCEELGALLGRMHLAGRSYGAYLENPRGPRWWRFAAREVMPFLDS
                     SKKNLLESELAYQAEQRFPDLPRGPVHADLFRDNTLWDHARISGVVDFYFAGVDCLLY
                     DVAVCANDWCLRPDSFLDGDRVAALLSSYHSVRPFTALERSAWRPMLRAAALRFWLSR
                     LYDFHLPRPGVLVHAHDPEHFRRVLESRVAVDAPWIA"
BASE COUNT          385 a          646 c          720 g          250 t
ORIGIN      
        1 gagcacctcg tagagcatgc gcgccgaacg cccggtgcgg cgttccgcac gcagttcgct
       61 gatcaacttc cagccttcgg cgccgagcag gcgaagcgcg atctcgcggt cggagaacga
      121 ggtgtagttg taggggatct cgcggatgcg gttcatgagg tccccgggtc cgggcggaag
      181 acgtcacggt agctcgcgta gaagctcgcg aacagcgtcg gcagcagcac gatgaggagc
      241 ggaaacacga gcgacatcgc cggcacgccg agcgaggcga gcgccacgac cgacaccacc
      301 gcaagcggca gtgccagcgc gacgacagcc gtgaccgcgc cgtaggcgag gaaagcgcgc
      361 cagttcatga ggcaggcgaa aaagctgaag aacagcgcct tggcgggcgc ggtgccgtgc
      421 caggcagcga gcggcggcgc gaaccagaac atcatcagca ccggggtgta caaggccgcg
      481 gctgcgccga gcgcagcgag aaacgcatcg gagtgaagca cttcctcgtc tggccggcgg
      541 gcggagagca tccagccggc gagcgcacca ccatcggcga gcgcagtcgc ggcgagcacc
      601 agcgcaaggc aggcgaagta gcctacgccg aggatcagct ggctgcggag gtgattgcga
      661 aacccctcga agagcagcac gagctccacc ggacctttgc gcgcagccgc gcgcgccgcc
      721 gccatgaacc cgaccgagaa ggccggcacc atgatcgacg cggcggcaac gccgacaagc
      781 ggcacgaccg acaccagtgt catgagcagc cagtaggcga acaccatggc gagccacccg
      841 agtggcgcgg ccctgaacag gcgccagcct tcgagcagcc actgcgcgcc ccgcgccgtc
      901 tgcacgatgc gcgcctgcat caggcgatcc agggcgcgtc tacagcgacg cgcgactcca
      961 gcacgcgtcg gaagtgctcc gggtcgtggg cgtgcacgag cactcccggg cgcggcaggt
     1021 gaaagtcata gagtcgcgac aaccagaagc gcagcgccgc cgcacgcagc atcggccgcc
     1081 aggccgagcg ctcgagcgcg gtgaagggac gcacgctgtg gtagctggag agcagcgcag
     1141 caacccgatc gccgtccaga aagctgtcgg gccgcaggca ccagtcgttt gcgcatacgg
     1201 caacgtcgta gagcaggcag tccacgcctg cgaaatagaa gtccaccacg cccgaaatgc
     1261 gcgcgtggtc ccagagcgtg ttgtcgcgaa agagatcggc gtgcaccggg ccgcgcggca
     1321 ggtcgggaaa gcgctgctcg gcctggtagg cgagttcgct ctcgagaaga ttcttcttgc
     1381 tcgagtcgag gaatggcatg acctctcgcg cggcgaagcg ccaccatctc gggccgcggg
     1441 ggttctcgag gtatgcgcca taggagcgcc cggcgagatg catgcggcca aggagcgcgc
     1501 cgagctcctc gcaatgccgc gcgcccggcc cctcgagcga gcgcccgggt aggcgcgtga
     1561 ccagcgccgc cggcttgccg ttcaggcgcc cgaggtactg gtcggaaaga tcggcgatgg
     1621 gcgccgggca cgggatgccg tggcgtgcga gatgcgccat gagatcgaga taaaacggca
     1681 gctcctcggc cggcagccgc tcgaacagcg tcaggacgta gcggccctgg gtggtggtga
     1741 cgaaatagtt ggtgttctcg atccccgcct cgatgggctc gcacgcgacg agccggccga
     1801 cggagtagtt cctgagccat tcgtgcagct cggcctcgga gacgggggtg tagacggaca
     1861 aggcggggag aggctcagtc ccacgtgtgg atcggccaca tcggcacgcg caccccgtcg
     1921 tcgagcgaat cgcggcgcat ccagttgccg ttgcctgtgg tgtcgacgag gtagtagggc
     1981 tttccgtgcg gcggcgtcac c
//
LOCUS       DASUNT010000113         5184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_367428, whole genome shotgun sequence.
ACCESSION   DASUNT010000113 DASUNT010000000
VERSION     DASUNT010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367428"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..419
                     /locus_tag="VFX94_02280"
     CDS             165..419
                     /locus_tag="VFX94_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767037.1"
                     /translation="MILRAALALVLAVAATSAHAQSGKRPEGREQQMSREQRERMRDD
                     MREAYRPREETAQKTRQMSPEERGQLRRDIEDANRNLKRE"
     gene            complement(421..3204)
                     /locus_tag="VFX94_02285"
     CDS             complement(421..3204)
                     /locus_tag="VFX94_02285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5767038.1"
                     /translation="MVASSRRTPATGATARRKRAAASPSPHPPAQQALLGLTSDWYWE
                     QDTELRFTRVDVRNDSSAEHNLAKGILGKQRWETGLEIEGGWEAHRALLEARAPFRDV
                     LMWRNLPDGARRYISVSGEPTFDARGRFTGYRGIGRDISKQKRIQQLLKLDQAVTLRL
                     AEGNAPPVLNGALEAICNSLRWDCSLLWAPDEAAGVLKPLAGWATPENADAGRFLAGS
                     RDLSFAPGKGLVGAVWRSGEPLWSSDASADPRTLRGDLSRQTGLRAAALFPVHSGKRV
                     AAVLEFTARRMRQPDKRLWQTLGAIGTQVGQFLSRLEAERAVRESEARFRALTNLSSD
                     WYWELDAEYRFTRLEGREVAGGDPGLLQRLVGARRWETELEVEGGWEVHRAILDARQP
                     FHDVLMWRRMQDGSVRYMRISGEAVFNADGSFSGYRGVGRDVTEEKRAEQMLRLEHEV
                     ARLLAGAEDASGGLKGVIRAVCEAEGFACGRYFRIEGEIARLQDAWAISEPAIAPFIE
                     RSRTFVFRAGEGLTGTVWLSGEALWSADIMRDPRVRDRNAWHGTGLRGGFAFAVRAEG
                     RTIGVLNFSSLKPREPDQRLLAASRVIGSQIGQFLQRKQAEAALRESEARFRSLTQMS
                     SDFFWETDEAHRFTQLVHGPEYLPPEMSRGVIGKTAWELPNESPDEAGWAAHRAELDQ
                     HQPFREFEFARRMPDGVVRFLSISGDPRFSPEGAFIGFRGVGRDITEIALARKHIASL
                     AYSDPLTGLANRTSLMPALDQAVQRARRRNSKIAVVILDLDGFKEINDAYGHDAGDAL
                     LVELAGRLRDNLRASDLIGRLGGDEFLVVLEEVQDTEPVDVVVRKLLAETVRPYTLPG
                     ARASVTASIGISIFPDDAADAAALMKHADIAMYAAKQAGKNTWRFYSSGPAANDPSQA
                     MARSGATNSGTEP"
     gene            complement(3271..4665)
                     /locus_tag="VFX94_02290"
     CDS             complement(3271..4665)
                     /locus_tag="VFX94_02290"
                     /inference="COORDINATES: protein motif:HMM:NF039580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX5767039.1"
                     /translation="MTATVALPFWLVVAIGVLALWALYEHVLMPALRWMLSQPANRVL
                     DDVSTRLRIGIRPFQRTKRQALVHRLLTDPKVQQAAEQFSRERRLPLPEVLQTVEGYA
                     REIVPAFNAYLYFRFGYWIGRRVAKLLYRVRVGYVDSAGLERIDPDATVVCVMNHRSN
                     MDYVLAGFIAADQAAVSYAVGEWARIWPLSALIRAMGAYFVRRNSRDELYRRVLERYV
                     AMATEAGVPQAVFPEGGLTRDGRMREPKLGVIDYMLRGFRIGGERDLVFVPLGINYDR
                     VLEDRSLLKETGSTPKAMGLWITLRFVVHNLVLMLRSEWHRFGYACVNFGTPISMREY
                     CRARGIDFQALADEERRAAIARLGRELMQAVGSIVPVVPVPLIATVFVAHPRRALSAL
                     ELKSEVGRLLSRLESARAHVYIPRGDFDYALTVGLRMLRLRRLIDEEDGLFRARPHAL
                     PLLAYYANSVAHLI"
     gene            complement(4662..>5184)
                     /locus_tag="VFX94_02295"
     CDS             complement(4662..>5184)
                     /locus_tag="VFX94_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5767040.1"
                     /translation="SSALLGAKLDFGRWVAVLAGFGGVLVIIRPWGAEFHPAMLASVV
                     NAILYAIFMMMTRRLAAYDSPETIQYLPAVGAALGLMPFALAAWESPSGWLEWTLACL
                     MGMLGGFGHHLLALAHRYAPAHVIAPFLYQQVIYMALFGYLVFGDVPSPGLWLGAAIV
                     IASGLYLFYRERR"
BASE COUNT          858 a         1772 c         1783 g          771 t
ORIGIN      
        1 gcaccagcag ctcgcgcagg cgctgatcgt catcgaccac gagaatgcgc gttttcagct
       61 caggcatacc ggccggcttt tctgcagcat ggatctttca tcgggcaata gattgttaca
      121 aaagcacacc cggccgcaag gcgggccgcg taacatgccg cttcatgatc ctgcgtgccg
      181 cgctcgccct cgtgctcgcc gtcgctgcga cgagcgcgca cgcgcaatca ggcaagcgtc
      241 ccgaaggccg ggagcagcag atgagccgcg agcagcgcga gcgcatgcgc gacgacatgc
      301 gcgaggccta ccgcccgcgc gaggagacgg cgcaaaagac gcggcagatg tcgccggagg
      361 agcgcggcca gcttcgacgc gacatcgagg atgcgaaccg caacctgaag cgcgagtgaa
      421 tcagggctcc gtccccgaat tcgttgctcc ggaacgcgcc atcgcctgcg acggatcgtt
      481 cgcggcgggc cccgaggagt agaagcgcca ggtgttcttg ccggcctgct tcgcggcgta
      541 catggcgatg tcggcatgct tcatgagcgc ggcagcgtcg gcggcgtcgt cggggaagat
      601 gctgatgccg atgctcgcgg tgacgctcgc ccgcgcgccc ggcagcgtat agggccgcac
      661 ggtctcggcg aggagcttcc tcaccactac gtcgaccggc tcggtatcct gcacttcctc
      721 cagcacgacg aggaactcgt cgccgcccag gcgtccgatg aggtcgctcg cgcgcaggtt
      781 gtcgcgcagc cgcccggcga gctcgacgag cagcgcgtcg cccgcgtcgt ggccataggc
      841 gtcgttgatc tccttgaagc cgtccaggtc gaggatcacg acggcgatct tggagttcct
      901 gcgccgcgcg cgctgcactg cctggtcgag cgcgggcatc aggctcgtgc ggttggcaag
      961 tccggtcagc ggatcgctgt acgcgagcga ggcgatgtgc ttgcgcgcaa gcgcgatctc
     1021 ggtgatgtcg cgcccgacgc cgcggaagcc gatgaatgcg ccctccgggc tgaagcgcgg
     1081 atccccgctg atcgagagga agcgtaccac gccgtcgggc atgcggcgcg cgaactcgaa
     1141 ttcgcggaac ggctgatgct gatcgagctc cgcgcggtgc gccgcccagc ccgcctcgtc
     1201 ggggctttca ttcggcagct cccacgccgt cttgccgatg acgccgcggc tcatctcggg
     1261 cgggaggtat tccgggccgt gcacgagctg ggtaaagcgg tgcgcctcgt cggtctccca
     1321 gaagaagtcc gacgacatct gggtcaggct gcgaaaccgc gcctcgctct cccgcagcgc
     1381 ggcctcggcc tgctttctct gcaggaactg gccgatctgg ctgccgatga cgcgcgaggc
     1441 ggcgaggagc cgctggtcgg gctcgcgcgg tttcaggctc gagaagttca gcacgccgat
     1501 cgtacggccc tcggcgcgca ccgcgaaggc gaagccgccg cgcagccccg tgccgtgcca
     1561 cgcgttgcgg tcgcgcaccc gcgggtcgcg catgatgtcg gccgaccaca gcgcttctcc
     1621 cgacagccac accgtgccgg tgaggccctc gccggcgcgg aagacgaacg tgcgcgagcg
     1681 ctcgatgaac ggcgcgatcg ccggttcgct gatggcccac gcgtcctgca agcgcgcgat
     1741 ctcgccctct atgcggaaat agcgaccgca ggcgaagcct tcggcttcgc ataccgcgcg
     1801 gatcacccct ttcaggccgc cggaggcgtc ctcggccccg gcgagcagcc gcgccacctc
     1861 gtgctcgagc ctgagcatct gctcggcgcg cttttcctcg gttacgtcgc ggccgacgcc
     1921 gcgatagccg gagaagctgc cgtcggcatt gaacaccgct tcgccgctta tccgcatgta
     1981 gcgcaccgag ccatcctgca tgcgccgcca catgagcaca tcgtggaacg gctgcctggc
     2041 gtcgaggatg gcgcggtgca cttcccatcc gccttccacc tcgagctcgg tttcccagcg
     2101 cctcgcgccg accaggcgct gcagcagccc ggggtctccg ccggcgacct cgcggccttc
     2161 gaggcgcgtg aagcggtatt ccgcatcgag ctcccagtac caatcggagg acaggttggt
     2221 gagcgcgcga aagcgcgctt cgctttcacg caccgcacgc tcggcctcga ggcggctcag
     2281 gaactggccg acctgcgtgc cgatcgcccc gagggtctgc cacagccgct tgtcgggctg
     2341 gcgcatgcgc cgcgcggtga attccaggac cgcggcgacg cgcttgccgg agtgcaccgg
     2401 gaaaagcgca gccgcgcgca gcccggtttg gcgcgagaga tcccctcgca gcgtgcgggg
     2461 atcggcgctg gcgtcgctgc tccacagcgg ctcgcccgag cgccatacgg cgccgacgag
     2521 tcctttgccg ggagcgaacg acagatcgcg cgatccagcg aggaagcggc cggcgtctgc
     2581 attctcgggc gtcgcccagc cggcgagcgg cttcagcacg cccgccgcct cgtcgggcgc
     2641 ccagagcagc gagcagtccc agcgcagcga gttgcagatc gcttcgagcg ctccgttcag
     2701 caccggcggc gcattgcctt cggccaggcg cagcgtgacg gcctggtcga gcttgagaag
     2761 ctgctggatg cgcttttgct tgctgatgtc gcggccaatg ccgcgatagc cagtgaagcg
     2821 gccgcgcgca tcgaacgtcg gctcgccgct gacgctgata tagcggcgcg cgccgtcggg
     2881 caggttgcgc cacatcagga catcgcggaa aggggcgcgc gcctcgagca gcgcgcggtg
     2941 ggcttcccag ccgccctcga tctccagccc ggtctcccag cgttgcttgc cgaggatgcc
     3001 cttggccagg ttgtgctcgg cgctcgagtc gttgcgcacg tcgacgcgcg tgaaccgcag
     3061 ctccgtgtcc tgctcccagt accagtcgga cgtgaggccg agaagggctt gctgcgctgg
     3121 cggatgcggc gagggcgaag ccgcagcccg cttgcgccgt gcggtggcgc ctgtcgctgg
     3181 cgtgcgtctc gatgaagcca ccatggtctc gattccctcc cgcggtcaat ctcctggacg
     3241 cttatccggg cgtccagcgc atcatccgct ctatattaga tgagcgaccg agttcgcata
     3301 gtaggcgagg agcggaagcg cgtgcggccg cgcgcgaaac aagccgtctt cttcatcgat
     3361 aaggcgacgc agccgcagca ttcgcaggcc gacggtcagc gcgtaatcga agtcgccgcg
     3421 cgggatgtag acgtgcgcgc gcgcggactc gagccgcgag agaagccgcc ccacctcgga
     3481 cttgagctcg agcgcagaga gcgcgcgccg ggggtgcgcg acgaacaccg tggcgatcag
     3541 cggcaccggc acgacaggca cgatggagcc caccgcttgc atcagctcgc gcccgaggcg
     3601 cgcgatcgcg gcgcggcgct cttcgtcggc aagcgcctgg aaatcgatgc cgcgggcgcg
     3661 gcagtactcg cgcatcgaga tcggcgtgcc gaagttcacg caggcgtagc cgaagcgatg
     3721 ccattcgctg cgaagcatga ggaccaggtt gtggaccacg aagcgcaagg tgatccacag
     3781 ccccatggcc tttggggtgg agccggtttc cttcaagagc gatcggtctt caagaacgcg
     3841 gtcgtagttg atgccgagcg gcacgaacac aaggtcgcgc tccccgccga tgcgaaagcc
     3901 gcgcagcatg tagtcgatca cgccgagctt cggctcgcgc atgcgtccgt cgcgggtcag
     3961 gccgccttcc gggaacacgg cctggggcac tccggcctcg gtcgccatgg cgacatagcg
     4021 ctcgagaacg cgccggtaga gctcgtcgcg cgagttgcgg cgcacgaaat acgcgcccat
     4081 cgcccggatc agcgcggaga gcggccagat gcgcgcccat tcgcctactg catacgagac
     4141 ggccgcctgg tcggcggcga tgaagccggc cagcacgtaa tccatgttcg agcggtggtt
     4201 catgacgcac acgaccgtgg cgtcagggtc gatgcgctcg agtcccgcgg agtccacgta
     4261 gccgacgcgc acgcggtaga ggagcttcgc cacgcggcgg ccgatccagt agccgaagcg
     4321 aaagtaaagg taggcgttga aggcaggcac gatctcgcgc gcataccctt cgaccgtctg
     4381 cagcacttcg ggcagcggca gccgccgctc gcgggaaaac tgctccgccg cctgctgcac
     4441 cttcggatcg gtgagcagcc ggtggacgag cgcctggcgc ttggtgcgct ggaacgggcg
     4501 gatgccgatt cgaaggcgcg tcgagacatc gtcgagcact cggttcgccg gctggctgag
     4561 catccagcga agcgccggca tcaggacgtg ctcgtacagc gcccacagcg ccagcacgcc
     4621 aatcgctacc acgagccaga acggcagcgc gaccgtagcg gtcatcgtcg ctcgcggtag
     4681 aagaggtaaa gcccgctcgc gatcacgatc gccgcgccga gccacaggcc gggactcggg
     4741 acatcgccga acacgaggta gccgaatagc gccatgtaga tcacctgctg gtacaggaac
     4801 ggcgcgatca cgtgcgcggg agcatagcgg tgcgcgagcg cgagcaggtg gtggccgaag
     4861 ccgccgagca tccccatgag gcaggcaagc gtccattcga gccagccgct gggcgattcc
     4921 catgccgcca gcgcgaaagg catgaggccg agcgcggcgc cgaccgccgg aaggtactgg
     4981 atcgtctcgg gcgagtcgta cgcggcaagc cggcgcgtca tcatcatgaa gatcgcgtac
     5041 aggatcgcgt tcaccaccga ggcgagcatc gccgggtgaa attccgcgcc ccagggccgg
     5101 atgatcacca gcacgccgcc gaagccggca agcacggcga cccagcgccc gaagtcgagc
     5161 ttcgctccga ggagcgccga gctc
//
LOCUS       DASUNT010000114         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_980292, whole genome shotgun sequence.
ACCESSION   DASUNT010000114 DASUNT010000000
VERSION     DASUNT010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980292"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VFX94_02300"
     CDS             <1..293
                     /locus_tag="VFX94_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX5767041.1"
                     /translation="RTERYEIALWKKIFYPIAALVMMALALPFAYMQARAGMVGVKVF
                     LGIMLGIFFHMLNSLFAHIGLLQNWPPLSAAVVPSAAFLTAAVLMMWWVERR"
     gene            complement(328..720)
                     /locus_tag="VFX94_02305"
     CDS             complement(328..720)
                     /locus_tag="VFX94_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX5767042.1"
                     /translation="MSGATPGLPRRLASMVYEAVLLFAVGFFAAWLFFFASGGREATS
                     GWLRHALQLFIVAVFAAYFLWCWLRSGQTLAMKAWGIRLVEVTPRRALVRFVLAAALL
                     PVSIVWALFDRDRQFLHDRLAGTRLVNA"
     gene            complement(717..1196)
                     /locus_tag="VFX94_02310"
     CDS             complement(717..1196)
                     /locus_tag="VFX94_02310"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HEX5767043.1"
                     /translation="MTRAAWLLLVALFSGPALAEPPVDITPKAKPAAKAKPAAKPKAQ
                     ARPAWAELTAEQQQILAPLKADWDSLEPERRRKWMGVAKRYPKMEPQAQERVQRRMQT
                     WAKLSPEQRRQARETYKQIVKAPPEKRAKLRQQWAEYQQLPQQERDSLAPQEEKRRR"
     gene            complement(1193..1573)
                     /locus_tag="VFX94_02315"
     CDS             complement(1193..1573)
                     /locus_tag="VFX94_02315"
                     /inference="COORDINATES: protein motif:HMM:NF023699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HEX5767044.1"
                     /translation="MNEMQFGHRIRQILNQGTGVSPKVAERLQRSRELALARQRPEPA
                     PAYAWADHALVRFGGWGGLALRVVLPLAMLIASAVAIYAWEQNQRAAELEEIDAQLLT
                     DDLPIDAYLDRGFQNWLKKRAAEQ"
     gene            complement(1570..2151)
                     /locus_tag="VFX94_02320"
     CDS             complement(1570..2151)
                     /locus_tag="VFX94_02320"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5767045.1"
                     /translation="MASRSELAAFLEGVEKRAFKQAVFAVRDEEAALDIVQDAMLRLA
                     EKYGHLPAGELPALFHRIVQNAIRDWFRRQKVRSMWTTLLSSVGPGREDDEDHDPLET
                     LDPTAGSEAREGPAAALEQAQVLEIIRQEVFRLPERQREAFMLRYWDELDVAETARKM
                     NCSEGSVKTHCSRAAHALQVALVARGITFETKR"
BASE COUNT          337 a          723 c          715 g          390 t
ORIGIN      
        1 agcgcaccga acgctacgag atcgcgctct ggaagaagat cttctacccg atcgcggcgc
       61 tggtgatgat ggcgctcgcg ctgccgttcg cgtacatgca ggcgcgcgcc ggcatggtcg
      121 gcgtcaaggt attcctgggc atcatgctcg gcatcttctt ccacatgctc aacagcctct
      181 tcgcccacat cggtctgctg cagaactggc cgccgctgtc ggcggcggta gtgcccagcg
      241 ccgccttcct caccgccgcg gtactgatga tgtggtgggt ggagcggcgc tgaaacgggt
      301 cagccatcgg tcctcgctcc tcgtttttca ggcgttgacg aggcgcgtgc cggcgaggcg
      361 gtcatgcagg aactgccggt cgcggtcgaa cagcgcccaa acgatcgaaa cgggtaagag
      421 cgctgcagcg agcacgaagc gcacgagcgc cctgcgtggc gtgacctcca ccaggcgaat
      481 gccccaggcc ttcatcgcga gcgtctgccc gctgcgcagc cagcaccata gaaagtaggc
      541 ggcgaagacg gcaacgatga agagctggag cgcgtggcgc agccagcccg aggtggcctc
      601 gcggccgccg ctggcgaaga agaagagcca cgctgcaaaa aagccgacgg cgaagagcag
      661 gaccgcttcg tagaccatgc tcgccaggcg tcgcggcaag cccggcgtgg cgccgctcaa
      721 cggcgccgct tttcttcctg gggagccagg ctgtcgcgct cctgctgcgg cagctgctgg
      781 tactcggccc actgctggcg cagcttggcg cgcttttccg gcggggcctt gacgatctgc
      841 ttgtaggtct cgcgcgcctg gcggcgctgc tccggcgaga gcttggccca agtctgcatg
      901 cgccgctgca cgcgctcctg ggcctgcggc tccatctttg gatagcgctt ggcgacgccc
      961 atccatttgc gccggcgctc cggctcgagg ctatcccagt cggctttgag cggcgccagg
     1021 atttgctgct gctcagccgt gagctcggcc caggccggcc tagcctgcgc cttgggcttc
     1081 gccgcgggct tggccttcgc cgcaggcttc gccttgggtg tgatatctac cggcggctcg
     1141 gcgagcgccg ggccgctgaa gagcgcgacc agcaggagcc aggcggcgcg cgtcattgct
     1201 ccgccgcgcg cttcttcagc cagttctgga agccgcgatc gaggtacgca tcgatcggca
     1261 ggtcgtcggt gagaagctgc gcgtcgatct cttcgagctc ggcggcgcgc tggttctgct
     1321 cccacgcgta gatggcgacg gcgcttgcaa tcagcatggc gagcggcagc acgacgcgca
     1381 gcgcgagacc gccccagccg ccgaagcgaa cgagcgcatg gtcggcccag gcataggcgg
     1441 gcgccggctc cgggcgctgg cgcgcgagcg ccagctcccg cgagcgctgc aggcgctcgg
     1501 cgactttcgg actgaccccg gttccctgat tcaggatttg acggatcctg tggccgaatt
     1561 gcatctcgtt catcgttttg tctcgaacgt gataccgcgc gccacgagcg ccacctgcaa
     1621 agcgtgagcg gcccgggagc aatgcgtttt cacgctgccc tccgagcagt tcatcttgcg
     1681 ggccgtttcg gccacgtcca gttcatccca ataacgcagc atgaaagctt cccgttgacg
     1741 ttccggcaag cgaaaaacct cttgcctaat gatttcaagg acttgagcct gctccagtgc
     1801 tgccgcaggg ccctctcttg cctctgatcc cgcggtgggg tccaaggttt cgagcgggtc
     1861 gtggtcctcg tcatcctcgc ggccggggcc gacggaggaa agtagcgtag tccacatcga
     1921 acgcactttt tgacgccgaa accagtcgcg gatggcgttc tgcacgatgc ggtggaaaag
     1981 cgccggcagc tcgcccgccg gcaggtggcc gtatttctcc gcgagccgca gcatcgcgtc
     2041 ttgcacgatg tcgagcgccg cctcctcgtc gcgaacggcg aacaccgcct gcttgaatgc
     2101 gcgcttctcg acgccttcca ggaaagcggc aagctcgctt cgggatgcca ggttggtccc
     2161 tctat
//
LOCUS       DASUNT010000115         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_717580, whole genome shotgun sequence.
ACCESSION   DASUNT010000115 DASUNT010000000
VERSION     DASUNT010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717580"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VFX94_02325"
     CDS             <1..701
                     /locus_tag="VFX94_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEX5767046.1"
                     /translation="RDITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHALEAA
                     GKSFSFDLSNHMVIIGLFIGGLVPYLFGAMAMEAVGRAAGSVVLEVRRQFKEIPGIMQ
                     GTAKPQYGTAVDMLTRAAIREMMIPSLLPVLVPIVVGLVLGPQALGGVLVGTIITGLF
                     VAISMCTGGGAWDNAKKYIEDGHHGGKGSDAHKAAVTGDTVGDPYKDTAGPAVNPLIK
                     IINIVALLVVPLMV"
     gene            803..2317
                     /gene="ggt"
                     /locus_tag="VFX94_02330"
     CDS             803..2317
                     /gene="ggt"
                     /locus_tag="VFX94_02330"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5767047.1"
                     /translation="MVAAASPLAVQAGVEVLERGGSAVDAAIAVQMVLGLVEPESSGI
                     GGGAFMLHWSQDEKKLRAYDGRETAPAAARADRFLDSQNKPLSFMEAAVGGRSVGVPG
                     VLRMLELAHQRHGRRPWNELFGAAIRIAEEGFDASPKLQEALSRERFLREDQAAKKVY
                     YSGPRIVNTAYAETLRVIALGGAGVFYEGDIAKDIVLAVRTHEKPGDLTAADLRTYRA
                     LEREAVCGLYRSRRVCSMGPPSSGGVAVLQILGVLERTSFARAPAQSGAALHLFAEAG
                     KLAYADRARYLGDPDFVAVPVKALISPSYLEKRAKLVTERAMALAAPGDPEAGTSHFS
                     VVDSRGDIVSMTTTIEAVFGSRVMVRGFLLNNQLTDFDFLPAQANSVAPGKRPRSSMA
                     PTIAFLNDQPMLAVGSPGGSMIINYVAKALVGTLDWGLDIQSAIELPNFGSRNGPTLI
                     EQGSGYEKLAAGLAARGHQIETAPLTSGLHGIERVAGGWRGGADPRREGVVRGR"
     gene            complement(2451..>2735)
                     /locus_tag="VFX94_02335"
     CDS             complement(2451..>2735)
                     /locus_tag="VFX94_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEX5767048.1"
                     /translation="AILACSALKATYRERLAQGIEDFTTVYLDGDFALINQRMKSRRH
                     RYMPAALLESQFAALEPPADAIAIDVAATPDDCVAAIVARLTERRTSGSA"
BASE COUNT          450 a          877 c          947 g          461 t
ORIGIN      
        1 tgcgcgacat caccgatccg ctggacgcgg tgggcaacac caccaaggcg gtgaccaagg
       61 gctacgccat tggttcggcc gggcttgccg cgctcgtgct cttcgccgac tacacgcatg
      121 ccctcgaggc ggccggaaag agcttcagct tcgatctctc caaccacatg gtgatcatcg
      181 gcctctttat cggcggcctg gtgccgtacc tattcggcgc catggcgatg gaggccgtcg
      241 gccgcgcggc gggatcggtg gtgctcgagg tgcggcgcca gttcaaggaa atccccggca
      301 tcatgcaggg cacggcgaag ccgcagtacg gcacggcggt cgacatgctt acccgcgcgg
      361 cgatccgcga gatgatgatc ccctcgctgc ttccggtgct ggtccccatc gtcgtagggc
      421 tggtgcttgg gccgcaggcg ctcggcggcg tgctcgtggg caccatcatc actggcctct
      481 tcgtcgccat ctcgatgtgt accggcggcg gtgcatggga caacgcgaag aaatacatcg
      541 aggacggtca tcacggcggc aaggggtccg atgcgcacaa ggccgcagtg accggcgaca
      601 cggtcggcga tccctacaag gacaccgccg gcccggccgt caacccgctc atcaagatca
      661 tcaacatcgt cgcgctactc gtcgtacccc tgatggtgtg aacgggcttc tcaccgcaat
      721 tgcacgaagg gcggcgctcg ccgccctttt tgtttgctgc tcctgcgcgc aagcgcgcga
      781 ggccgtagtt gcgaaggaat tcatggtcgc agccgcgagc ccgctcgctg tgcaggccgg
      841 cgtagaggtg ctcgagcgcg gcggctcggc ggtcgatgcg gcgatcgccg tgcagatggt
      901 gctcggcctg gtcgagcccg agtcttcggg catcggcggc ggcgcgttca tgctgcactg
      961 gtcgcaggac gagaagaagc tgcgggccta cgacggccgc gagacggcgc ccgcggccgc
     1021 acgggcagat cgtttcctgg actcgcagaa caagccgctc tctttcatgg aagctgcagt
     1081 cggtgggcgc tcggtcggcg tgccgggcgt gctgcgcatg ctggagctcg cgcaccagcg
     1141 ccacgggcgc cggccgtgga acgagctctt cggcgccgcg atccgcatcg ccgaagaggg
     1201 cttcgacgcc tcgccgaagc tgcaggaagc gctatcgcgc gaacggttcc tccgggaaga
     1261 tcaagctgca aaaaaggtct attactcggg tccgcgaatc gtgaacacag cctatgccga
     1321 gacgcttcga gtcatagcac taggcggcgc cggggttttt tacgagggcg acatcgcgaa
     1381 ggacattgtg ctcgcggtgc gcacgcacga aaagcccgga gacctcaccg cagcggatct
     1441 gcgtacctac cgcgcgctcg agcgcgaggc ggtgtgcggc ctgtatcgaa gccggcgcgt
     1501 ctgctccatg ggtccgccga gctcgggcgg cgtcgccgtc ctgcagatcc tcggggttct
     1561 cgagcggact agctttgcgc gcgcgcctgc gcagtcgggg gcggcgctgc acttgttcgc
     1621 cgaggcggga aagctggcct acgccgaccg cgcgcgctac ctgggagatc cagactttgt
     1681 cgcagttccc gtaaaagctc tgatatctcc ttcttatctg gaaaagcgcg caaagctggt
     1741 caccgagcgc gccatggcgc tcgcagcgcc tggagacccg gaagcgggga cgagccactt
     1801 ctccgtcgtc gactcgcgcg gcgacatcgt ttcgatgacg accacgatcg aagcggtttt
     1861 cggcagccga gtcatggtgc ggggcttcct cctgaacaat cagctcaccg acttcgactt
     1921 cctgcccgcg caggcgaaca gcgtcgcgcc cggcaagcgc ccgcgcagca gcatggcacc
     1981 caccattgct tttctgaatg atcaaccgat gctggcggtc ggctcgccgg gcgggtccat
     2041 gatcatcaac tatgtcgcca aggctctcgt cggcacgctc gactgggggc tcgacatcca
     2101 gtcggcgatc gagttgccca atttcggctc gcgcaacggc ccgacgctga tcgagcaggg
     2161 cagcggctac gagaagctgg cggccgggct tgcagcgcgc gggcatcaaa tagagacggc
     2221 tccgctgacg agcgggctgc acgggatcga gcgggttgcc ggggggtggc gcggcggcgc
     2281 cgacccgcgg cgcgagggtg tcgtccgagg ccggtgagcg ggttgtaagc catcggtaag
     2341 cttgatgtcg cgatggatga agccccgcgt cgtgcacgag ctccaggcca tcgaggcgcc
     2401 ggaacgtgca gcttttccaa cggcgcgcgc tacaaggtgc acatcggcaa ttaggcggat
     2461 cctgaagtac ggcgctccgt aaggcgcgcg acgatcgcgg ccacgcagtc gtccggcgtg
     2521 gcggcgacgt cgatcgcgat cgcatccgcc ggcggctcga gcgcggcgaa ctggctctcg
     2581 agaagcgcgg caggcatgta gcggtggcgg cggcttttca tgcgctggtt gatcagcgcg
     2641 aagtcgccgt cgaggtagac ggtggtgaag tcctcgatgc cctgcgccag gcgctcgcgg
     2701 taggtcgctt tcaaggccga acacgcgagg atggc
//
LOCUS       DASUNT010000116         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1068534, whole genome shotgun sequence.
ACCESSION   DASUNT010000116 DASUNT010000000
VERSION     DASUNT010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068534"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2179)
                     /locus_tag="VFX94_02340"
     CDS             complement(<1..>2179)
                     /locus_tag="VFX94_02340"
                     /inference="COORDINATES: protein motif:HMM:NF017857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HEX5767049.1"
                     /translation="YGDYRDKSFDLKVQLLPERGWWPALGVGMQDIAGGTGIFSAPYA
                     VASKRLGPLDLTLGYGGDRIDGFFGGARLELGPNWGLVAEYDAYNYSQDLGSTLSGAA
                     SYRKEPAFGIEYRREFWGAKAFTSHDELGFNAYLQVPLERREFVPKVNEPAPYTKINP
                     RPTEKQWDNDPEHRARLGRALAEQDFRGERLVYENGVLSAVLTNTRISSMPRAVGRAA
                     RTLLSFAPLEVREIRVTYVQGSLPVATYTFVNIPLLQRYFNGMASREQLAPYVAIEYA
                     KPVDVEEEKKQMLAGFDEPLPEGIVMQRQGGEFLALGENFAGGRLRVRPGLGLFFNDP
                     SGVLKYDLYVAAIYNRALARQLFLQAETRLTLAEDVSDVTQASNSELPHVRSDIALYK
                     QGNDFKLTRLLVNRFFHPDRRMYARLSAGIYEEMYSGVGGQALYLGRDGDWAADVAVD
                     WVRQRDYEGGFGHLDYKTVTAIGSLHYRMAERVTATVRAGRFLAKDEGARFELKRRFA
                     SGFEVGAWYTVTNGNDITSPGSPSNPYYDKGIFLAMPLDPLLSYDSQAAAGFSLSPWT
                     RDVGQMVASPGDLARILERPIVQMHTLDGLSRFGDRDDDYDLPKLGADRRWPDFLLQD
                     ARGVSAAAGEIDWVRTSALAGAILLSFASLDREVFEFADKRKDKEWLKDTVKIGDALP
                     IAAVGLSGVFAFDESRPNLSRAGVAALEASALAFVASTGLKYAA"
BASE COUNT          370 a          735 c          724 g          350 t
ORIGIN      
        1 ggccgcgtac ttcaagcccg tcgaggcgac gaaggccagc gccgaggcct cgagcgccgc
       61 tacgccggcg cgcgaaaggt tcggacgcga ctcgtcgaag gcgaacacgc ccgagaggcc
      121 gaccgcggcg atcggcagcg catcgccgat cttcaccgtg tctttgagcc actccttgtc
      181 cttgcgcttg tccgcgaatt cgaacacctc ccgatccagg gaggcgaagg aaaggaggat
      241 cgccccggcc agcgcgctcg tccgcaccca gtcgatctcg cccgcggcgg cgctcacgcc
      301 gcgcgcgtcc tgcagcagga agtcgggcca gcgccggtcc gcgcctagct tcggtaggtc
      361 gtagtcgtcg tcgcggtcgc cgaagcgcga gaggccgtcg agcgtgtgca tctgcacgat
      421 gggacgctcg agaatgcgcg cgagatcgcc cggcgaggcg accatctggc cgacgtcgcg
      481 cgtccagggc gagagcgaaa agccggccgc agcctgcgag tcgtaggaaa ggagcgggtc
      541 gagcggcatc gcgaggaaga tgcccttgtc gtaatacggg tttgaaggcg atccgggcga
      601 ggtgatgtcg ttgccgttcg tcaccgtgta ccaggcgccg acctcgaacc ccgaagcgaa
      661 gcgccgcttg agctcgaagc gcgcgccctc gtccttggcg aggaagcgcc cggcgcgcac
      721 cgtggcggta acgcgctcgg ccatccggta gtggagcgag cctatcgcgg tgaccgtctt
      781 gtaatcgagg tggccgaacc cgccctcgta gtcgcgctgc cgcacccagt cgaccgcgac
      841 gtccgcggcc cagtcgccgt cacgccccag atacaaggcc tgcccgccca cgccgctata
      901 catctcttcg tagatgccag cggagagacg cgcgtacatg cgccggtccg gatggaagaa
      961 acggttcacg agcaggcgcg tgagcttgaa gtcgttgccc tgcttgtaga gcgcgatgtc
     1021 ggagcgcacg tgcggcagct cgctgttcga agcctgcgtc acgtcgctca cgtcctccgc
     1081 gagcgtgagc cgcgtttcgg cctggaggaa gagctggcgc gcgagcgccc ggttgtagat
     1141 cgccgccacg tagaggtcgt acttgagcac gccgctcgga tcgttgaaga agagcccaag
     1201 ccccgggcgc acgcgcagcc gcccgcccgc gaagttctcg ccgagcgcaa ggaactcccc
     1261 gccctgccgc tgcatgacga tgccctcggg cagcggctcg tcgaaacccg ccagcatctg
     1321 ttttttttct tcttccacat ccacgggctt cgcatattcg atggcaacgt acggcgcgag
     1381 ctgctcgcgc gaagccatgc cgttgaagta gcgctgcaga agcgggatat tgacgaaggt
     1441 gtacgtggcc accggaagcg agccctgcac gtaagtgacg cggatctcac gcacttcgag
     1501 cggggcaaat gagagcagcg tgcgcgccgc gcgccccacc gcgcgcggca tcgacgagat
     1561 gcgcgtgttg gtgagaacgg ctgacagcac gccgttctcg tagacgaggc gctcgccgcg
     1621 gaaatcctgc tccgcgagcg cgcgcccaag ccgcgcacgg tgttccggat cgttatccca
     1681 ctgcttctcg gtcggacgcg gatttatctt cgtgtacggc gccggctcgt tcactttggg
     1741 cacgaactcg cgccgctcga gcggcacctg gagataggcg ttgaagccaa gctcgtcgtg
     1801 cgatgtgaac gctttcgccc cccagaactc gcgccggtat tcgatgccga acgccggctc
     1861 cttccgatag ctcgctgcgc cggaaagcgt cgacccgagg tcctggctgt agttgtacgc
     1921 gtcgtactcg gcgaccaagc cccagttcgg tccgagttcg aggcgcgcgc cgccgaagaa
     1981 cccgtcgatt cgatcgccgc cgtagccgag cgtcagatcg agtggtccga ggcgcttcga
     2041 ggcgaccgcg tagggcgcgc tgaagatacc cgtgccgccc gcgatgtcct gcattcccac
     2101 accgagcgcc ggccaccagc cgcgctcggg cagaagctgc actttcaagt cgaacgactt
     2161 gtcgcgatag tcgccgtag
//
LOCUS       DASUNT010000117         2495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_630251, whole genome shotgun sequence.
ACCESSION   DASUNT010000117 DASUNT010000000
VERSION     DASUNT010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630251"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFX94_02345"
     CDS             complement(<1..678)
                     /locus_tag="VFX94_02345"
                     /inference="COORDINATES: protein motif:HMM:NF015410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5767050.1"
                     /translation="MARSTARLEQRIAEEQQAFARPLRSLVKRPPLLCAPGITVAEAA
                     ALMRAEDVGSLVVVDGGSRPIGIVTNQDIVAALAKNASGHAVVEAMTPNPFSLPGHAL
                     AYEAAVAMIAQRIRHVLVTEEGRLTGVVSERDLFSLQRLGLGELAMEIRLAASLEVLA
                     GLAGEVRGLTRTLVEQGVGAEQLTLFVSVLNDRISQRAIEIVRKRHDLERIRWCWLAF
                     GSEGRFEQ"
     gene            complement(681..>2495)
                     /locus_tag="VFX94_02350"
     CDS             complement(681..>2495)
                     /locus_tag="VFX94_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX5767051.1"
                     /translation="LKVESDGASEVVELAGAVNRLAEERRSLRRDVEARAREAGARLE
                     EERNRLAALMSELAEGVLVCNAEGRILLYNERARQLFSGEEALLGLGRSLFGLIDREQ
                     VAHARDKLQGGSALARFVTATPAGRLLRVQAAPVAGEAAIAGMVLTLEDVTGVLDREQ
                     QRLKLLHALTSSVRSPAANLRAAAENLASYPDMEPERRLRFIQIVATESALLSRSIDE
                     ALRGYADAVKASLTLSDMRAAELAGVARRRLESLPGLAVEIDSVSDELWLRVDSFAMI
                     QAFTFLCEKLRTEYGVRRVRFAARAKESFVELDLGWDGAIVASEALSLWETQPLGEGP
                     LTLRDVLERHGAEAWLHAAGPGGQRSGAVRFLLPAGEPAAAPKPPARAAESRPEYYDF
                     DLFRYGDSARELHERQLTDLSYTVFDTETTGLEPSAGDEIISIGAVRIVNGRLLKNEV
                     FERLVNPRRPLNRESARIHGIDAAALAAEPGIEEVLPAFHRFCEDTVLVAHNAAFDMR
                     FLELKEASTGVRFAQPVLDTLLLSAVLHPSLDDHRLESMADRLGVQVIGRHTALGDAL
                     LEAEIFLRLLPLLAARGIVTLRQALEASRETYYSRLRY"
BASE COUNT          378 a          913 c          846 g          358 t
ORIGIN      
        1 ctgctcgaag cgcccttcgc tgccgaaggc aagccagcac cagcggatgc gctcgaggtc
       61 gtggcgcttg cgtacgatct cgatcgcgcg ctggctgatg cgatcgttca gtaccgagac
      121 gaacagcgtg agctgctccg cgcccacacc ctgctccacg agcgttcgcg tcagcccgcg
      181 cacctcgccg gcgagcccgg ccagcacttc gaggctcgcg gcgaggcgga tctccatggc
      241 gagctcgccg aggccgagcc gctgcagcga gaaaagatca cgctcggaga ccacgccggt
      301 gaggcgtccc tcctcggtga cgagcacgtg gcggatccgt tgcgcgatca tggcgaccgc
      361 tgcctcgtag gcgagcgcat ggcccggcag cgagaacgga ttgggcgtca tggcttcgac
      421 gaccgcgtgc ccggaggcgt tcttcgcgag cgcagcgaca atgtcctggt tggtcacgat
      481 gccgatcggg cgcgagccgc catcgaccac gaccagcgat ccgacgtcct cggcgcgcat
      541 cagcgccgcc gcttccgcca cggtgatgcc gggcgcacag aggagcggcg ggcgcttcac
      601 cagagagcgc agcggccgcg cgaaggcctg ctgctcctcg gcgatgcgct gctccaggcg
      661 cgcggtggac cgggccatcg tcagtagcgc agccgcgaat agtaggtctc gcgcgacgcc
      721 tccagcgcct ggcgaagcgt cacgatgccg cgcgccgcga gcagcggcag caggcgcagg
      781 aagatctccg cctcgagcag cgcgtcgccg agcgcggtat gccgcccgat cacctgcacg
      841 ccgaggcggt cggccatgct ctccaggcgg tggtcgtcga ggctcggatg cagcaccgcc
      901 gagagcaaca gcgtgtcgag caccggctgg gcgaagcgca cgccggtcga ggcctccttg
      961 agctcgagaa agcgcatgtc gaaggccgca ttgtgcgcga ccaagaccgt gtcttcgcag
     1021 aagcggtgga acgcaggcag cacctcttcg atcccgggct cggcggcaag cgccgcggcg
     1081 tcgatgccgt ggatgcgggc cgactcgcgg ttcagcggtc ggcgcgggtt gaccagccgc
     1141 tcgaacacct cgttcttgag cagcctcccg ttcacgatgc gcaccgcgcc gatgctgatg
     1201 atctcgtcgc ccgccgaagg ctcgaggccg gtggtctcgg tgtcgaacac ggtgtaggac
     1261 agatccgtta gctgccgctc gtgcaactcg cgcgccgagt cgccgtagcg gaacagatcg
     1321 aagtcgtagt actcgggccg gctctcggcc gcgcgcgctg gcggcttcgg cgcggctgcc
     1381 ggctcgcccg ccggcaggag gaagcggacc gcgccgctgc gctgcccgcc gggtcctgcg
     1441 gcatggagcc atgcctccgc cccatggcgc tccagcacat cgcgcaacgt gagcgggccc
     1501 tcgcccaggg gctgcgtctc ccacagcgaa agcgcctcgc tcgcgacgat cgcgccatcc
     1561 cagccgaggt cgagctcgac gaagctttcc ttcgcgcgtg cggcgaagcg cacgcgccgc
     1621 accccgtact cggtgcgcag cttctcgcac aggaatgtaa acgcctggat catcgcaaag
     1681 ctgtccacgc gcagccaaag ctcgtcgctc accgagtcga tttccactgc gagccccggc
     1741 aggctctcca gcctgcgccg cgcgaccccg gccagctcgg ccgcgcgcat gtcgctcagt
     1801 gtaaggctcg ccttcactgc gtcggcatag ccgcgcaacg cctcgtcgat cgagcgcgac
     1861 agcagcgccg actccgtggc gacgatctgg atgaagcgaa gccgccgctc gggctccatg
     1921 tcgggatagg aggcaaggtt ttccgcggcg gcgcgcaggt tcgccgccgg cgagcgcacg
     1981 ctcgacgtga gcgcatgcag cagcttcagg cgctgttgct cgcggtcgag cactccggtc
     2041 acatcctcca gcgtaagcac catccctgcg atcgcggctt cgcccgcgac cggcgccgct
     2101 tgcaccctga gcaggcgtcc cgcgggagtc gcggtgacga agcgcgcgag cgccgagccg
     2161 ccttgcagct tgtcgcgcgc gtgcgccacc tgctcgcggt cgatcaggcc gaagagcgag
     2221 cgccccaggc cgagcagcgc ctcctcgccg gagaagagct gccgcgcgcg ctcgttgtag
     2281 agaaggatgc gcccttcggc gttgcagaca agcacgcctt cggccagctc ggacatgagc
     2341 gcggcgagcc ggttacgctc ctcctcgagc cgcgcgcccg cctcgcgcgc ccgcgcctcg
     2401 acatcgcgcc gcaggctgcg ccgttcctcg gcgaggcggt tgaccgcgcc cgccagctcc
     2461 accacttcgc tcgctccatc gctctccacc ttcag
//
LOCUS       DASUNT010000118         4453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1156605, whole genome shotgun sequence.
ACCESSION   DASUNT010000118 DASUNT010000000
VERSION     DASUNT010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156605"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VFX94_02355"
     CDS             complement(<1..985)
                     /locus_tag="VFX94_02355"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004544727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX5767052.1"
                     /translation="MCGILGIVARSPVNQLLYDGLLLLQHRGQDAAGIVTAERNSFHM
                     HKAPGMVRDVFRTRNMRSLPGNIGIAHCRYPTAGSAFSSAEAQPFYVNSPFGIVLGHN
                     GNLTNAEQLKEEMFRTDLRHINPGSDSEVLVNVLAHELEQASVKLHLDPQTIFSAVAA
                     VHRRVRGAYAVVAMIAGYGVLAFRDPYGIRPAVIGYNETPVGTEYMVASESVAVDALG
                     FRVLRDVGPGEAIFIDEEGNFYSEQCAPHASLNPCIFEYVYLARPDSLIDGASVYESR
                     LNMGEALAAKIRRQYRHLQVDVVIPIPDSSRPSALQLAAGLGVPYREGFVKN"
     gene            complement(1015..1503)
                     /locus_tag="VFX94_02360"
     CDS             complement(1015..1503)
                     /locus_tag="VFX94_02360"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX5767053.1"
                     /translation="MTWVDYTVIAILALSIAWGAWRGLVHETLSLAGWIMAFVAANLL
                     AGPLAEHFPANMRPEFRVVSAFVAVFVATLIVATLLSALVTKFVKVSVLHGLDRWLGA
                     LFGLLRGLVIVIALAMLAGLTPLPRSSDWKDSALGYSLAQTVIQLKPWLPPAFANRLK
                     YN"
     gene            complement(1516..1968)
                     /locus_tag="VFX94_02365"
     CDS             complement(1516..1968)
                     /locus_tag="VFX94_02365"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX5767054.1"
                     /translation="MAQEQQDVETLKRRGRRRLVGAVALMLAAVIVLPMVFDTEPRGT
                     GSTLSVRIPSEDTPFTPKAPAKKPEEKKAEKPATDEYVVQVGAFTSPQPVIAKLKAAK
                     VPYYTEVVNNNLTRVRAGPFPTREAAMQAIEQLKALGFAPGPVASKSG"
     gene            complement(1935..3197)
                     /gene="folC"
                     /locus_tag="VFX94_02370"
     CDS             complement(1935..3197)
                     /gene="folC"
                     /locus_tag="VFX94_02370"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEX5767055.1"
                     /translation="MQAVRRSLSDWLAFIEGQHPRSIALGLERVKQVLDRMSIAIRCP
                     VITVGGTNGKGSTCAMLESMLRAAGYRTGLYTSPHLVRYNERVRIEGAEADDSLLVES
                     FAVVENSRGEVPLTYFEFGTLAAFWAFSENNLETAILEVGLGGRLDAVNALDADCAVL
                     TSVGIDHVDYLGPDRESIGREKAGIFRAGRPAVLAEPDPPQSVRQAVAEKLFLGKDFG
                     FTVHDAQWNYWGPKGRRAGLAHPALRGAMQLRNASAALCALDALALPVAMQEVRRGLA
                     EVALPGRFQVLPGRPQVILDVAHNVEAAKVLADNLLASGYASETIAVCGMLRDKDIGG
                     VLRALAPRVTRWHFASLPGPRGASAEQLEQKLSESRAVVEEFASPTAAFIAARERAEE
                     GDKIVVFGSFLTVGEVMAWLKNNKTSKR"
     gene            3571..3697
                     /locus_tag="VFX94_02375"
                     /pseudo
     CDS             3571..3697
                     /locus_tag="VFX94_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747404.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            3806..4243
                     /locus_tag="VFX94_02380"
     CDS             3806..4243
                     /locus_tag="VFX94_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX5767056.1"
                     /translation="MDFSLFPRDLFAELDRLQRQLQQSFELSPSIRGLARGAFPALNV
                     ATTPQSAEVYAFAPGLEPASIHVQIERGVLSISGERAAALPGDDEGASVHLNERFAGR
                     FHRVVSLTDDLDPGEVRATYREGVLHVSIQRHQAAQPRRIEVN"
BASE COUNT          742 a         1517 c         1467 g          727 t
ORIGIN      
        1 ggttcttgac gaacccttcg cggtacggca cgccgagccc ggcggcaagc tgcagcgccg
       61 aaggccggct cgagtcgggg atcgggatca ccacgtccac ctggaggtgg cggtactggc
      121 gccggatctt ggccgcgagc gcctcgccca tgttgaggcg cgactcgtag accgaggcgc
      181 cgtcgatcag cgagtcgggg cgggcgaggt agacatattc gaagatgcag gggttcaggc
      241 tggcgtgcgg cgcgcactgc tcgctgtaga aattgccctc ttcgtcgatg aagatcgcct
      301 cgcccgggcc gacgtcgcgc agcacgcgaa agccgagcgc gtccaccgcg accgattccg
      361 acgccaccat gtattccgtg ccgacgggcg tttcgttgta tccgatcacc gccggccgga
      421 tgccgtacgg gtcgcggaac gccagtacac cgtagccggc gatcattgcg accacggcgt
      481 aggcgccgcg cacgcggcgg tggacggcgg ccacggcgct aaagatcgtc tgggggtcga
      541 ggtgcagctt caccgacgcc tgctcgagct cgtgcgcgag cacgttcacc agcacctcgg
      601 agtccgaccc ggggttgatg tggcgcaggt cggtgcggaa catctcttcc ttgagctgct
      661 cggcgttggt caggttgccg ttgtggccga gcacgatgcc gaacggcgag ttcacgtaga
      721 agggctgcgc ctcggctgag ctgaaggccg atcccgccgt cgggtagcgg caatgcgcga
      781 ttccgatgtt cccgggcagc gaacgcatgt tgcgcgtgcg gaacacgtcg cgcaccatgc
      841 ccggcgcctt gtgcatatgg aaggagttgc gctcggccgt gacgatgcct gcggcatcct
      901 gtccgcggtg ctggaggagc agtagcccgt cgtacagcaa ctggttgacg ggcgaacgcg
      961 cgacgattcc gaggatcccg cacatgggaa tgacttcccc tatgtggtta acggctaatt
     1021 atatttcaag cggttcgcga atgccggcgg cagccagggc ttgagctgga tcaccgtctg
     1081 ggcgagcgag taaccgagcg ccgagtcctt ccagtcggaa gagcgcggca gcggcgtgag
     1141 gcccgccagc atggcaagcg cgatgacgat cacgagcccg cgcagcaggc cgaacagcgc
     1201 gccgagccag cgatccaggc cgtgcagcac cgaaactttc acgaactttg tcacgagcgc
     1261 ggaaagcagc gtggcgacaa tcagcgtcgc gacgaagacg gcgacgaagg ccgagacgac
     1321 gcggaactcg gggcgcatgt tcgccgggaa gtgctcggcg agcggcccgg cgaggaggtt
     1381 ggcggcgacg aaagccatga tccagccggc gagcgagagc gtctcgtgca ccagcccgcg
     1441 ccacgcgccc caggcgatcg agagcgcgag gatcgcgatc acggtgtagt cgacccaggt
     1501 catcggcgct cgtccttatc ccgatttcga ggccaccggc cctggcgcaa agccgagtgc
     1561 cttcagctgc tcgattgcct gcatcgcggc ctcgcgggtg ggaaatgggc cggcgcgcac
     1621 gcgcgtgaga ttgttgttca ccacctcggt gtagtaaggc accttggccg ccttgagctt
     1681 ggcgatcacg ggctgcggac tcgtgaaggc gccgacctgg accacgtact cgtccgtggc
     1741 gggcttctcc gctttctttt cttcgggctt cttcgcagga gccttgggcg tgaacggcgt
     1801 gtcctcggaa ggaatgcgca cgctaagcgt cgagcccgtg ccgcgcggct cggtgtcgaa
     1861 caccatgggc agcacgatca ccgcggcgag catcagcgcc accgcgccga ccagccgcct
     1921 gcgtcccctg cgcttcagcg tttcgacgtc ttgttgttct tgagccatgc catcacttcg
     1981 ccgaccgtga ggaacgaacc gaacacgacg atcttatcac cctcctccgc acgctctcgc
     2041 gcagctatga aagcggcggt tggagacgca aactcttcca ctaccgctcg agattcactc
     2101 agtttttgtt ccaactgctc cgccgacgcg ccgcgaggcc ccggcagcga ggcgaaatgc
     2161 cagcgggtaa cgcgcggcgc aagcgcgcgc agcacgccgc cgatgtcctt gtcgcgcagc
     2221 atcccgcata cggcaatagt ttcgcttgca taacctgagg cgagcaggtt gtcggcgaga
     2281 acctttgccg cttcgacgtt gtgcgcgaca tctaggatga cctgcggcct gccgggcagc
     2341 acctggaagc gcccgggtag cgcgacctcg gcaagcccgc ggcgcacctc ctgcatcgcg
     2401 accggcagcg cgagcgcatc gagcgcgcac agcgccgccg aagcgttgcg tagctgcatc
     2461 gcgccgcgca gcgccggatg cgcgaggccc gcgcgcctgc ccttcggacc ccagtagttc
     2521 cactgtgcgt cgtgcaccgt gaagccgaaa tccttcccga ggaaaagctt ctccgctacc
     2581 gcctgccgca ccgactgcgg cgggtccggc tcggcaagga cggccggacg cccggcgcgg
     2641 aagatgcccg ctttctcgcg cccgatcgac tcgcgatccg gccccaggta atccacatga
     2701 tcgatgccga cgctggtcag cacggcgcaa tcggcgtcga gcgcgttcac cgcatcgagc
     2761 cggccgccga ggccgacttc caggatcgcc gtttcaagat tgttttccga gaaggcccaa
     2821 aaagcggcga gcgtgccgaa ttcgaaatag gtcagcggaa cctcgccacg cgagttttcg
     2881 accacggcga agctctccac gagcagcgag tcgtccgctt cggcgccctc gatgcgtacc
     2941 cgctcgttgt agcgcacgag gtgcggtgag gtgtacaggc cggtgcgata gccggcggcg
     3001 cgcagcatcg actcgagcat cgcgcaggtc gagcccttgc cgttcgtgcc gccgacggtg
     3061 atcaccgggc atcggatggc gatgctcatc cgatccagca cctgcttcac tcgttccaag
     3121 ccgagcgcga tcgaccgcgg atgctgacct tcgatgaaag cgagccagtc ggatagcgag
     3181 cgccgcaccg cttggatgat agcccgcccc tgtatgccgg ttgccgcccc ggcaaaatcg
     3241 gggtcagacc ccgattgtcc tagcgtttgg accagacggg cttcgccatc gcggagcccg
     3301 gcgggtagac gaccttgggc tagccgttct gccactgcac gatgaggaga tccgcccccg
     3361 cgcgccggcc cttcggtcgt gtccatcgcg cggatcgcct cggcgacctt cgtgcgatcg
     3421 gccgcgcccg ccttctcgag cgcgtccttg aagatccaca ggtcgccgta ggtcgagatg
     3481 ctgtcctggg tcatccacgg ctcgccgctg cgcttagtga actcctcgat cagcttctcc
     3541 tggcccttcg cgcttcgctc caaaggcctg acccttgcgc gggtcgccga gcggttcggc
     3601 tacgactctg aggcagcctt tcaaccgcgc cttcaagcgc gagttcggca tgccgccggc
     3661 gcagtggcgc cggcttcagc gggcgcccgc cggctagagc ttcacgcccc tcgtgcgcga
     3721 ccgggcgtcg gccgacccgg gggcttgaaa accgccgcgc cgcacctatc tgcggcttga
     3781 ccaaccgttt ggaggaggct tgtccatgga cttctcgctc tttccgcgcg accttttcgc
     3841 ggagctcgac cggctgcagc gccagctgca gcaatcgttc gagctctcgc ccagcatccg
     3901 cggcctcgcc cgcggcgcct tcccggcgct caacgtggca accacccccc agtcggccga
     3961 ggtctatgcg ttcgcccccg gcctggagcc ggcctcgatc cacgtacaga tcgagcgcgg
     4021 cgtgctcagt atctcggggg agcgcgcggc cgcgctcccc ggcgatgacg agggcgcgag
     4081 cgtgcatctg aacgagcgct tcgccgggcg cttccaccgc gtcgtctcgc tcaccgacga
     4141 cctcgacccc ggcgaggtgc gcgcgaccta ccgcgagggc gtgctgcacg tgagcatcca
     4201 gcgccatcaa gcggcgcagc cgcggcggat agaagtcaac tgacacggtg aacttgcaag
     4261 gtcaacagac aaggacaaga caatggacga gaccaacact cgcgtcgcgc agcaggcgac
     4321 gcaggccgcc gagcagcccg cgctgctccc gccggtcgat gtgatcgaag atgcggaagg
     4381 cattaccctc tacgccgacc tcccgggcgt gccgaaggag cgcctcgacg tgcacatcga
     4441 cggcgaggtg ctc
//
LOCUS       DASUNT010000119         3083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1244481, whole genome shotgun sequence.
ACCESSION   DASUNT010000119 DASUNT010000000
VERSION     DASUNT010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244481"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..141
                     /locus_tag="VFX94_02385"
     tRNA            67..141
                     /locus_tag="VFX94_02385"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99..101,aa:His,seq:gtg)
     gene            complement(134..1615)
                     /locus_tag="VFX94_02390"
     CDS             complement(134..1615)
                     /locus_tag="VFX94_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5767057.1"
                     /translation="MEPLQLLAQGFATCFTPLNLSFILIGVLIGQIIGALPGIGPAAG
                     MALLLPLTYGVPPVTAIMMLAGIMYGGQYGGTLTSVLVNVPGEAASVMTTIDGHQMAR
                     QGRAGAALSIAAIGSFIAGIAAVVAVVFVTPPLAAFALKFNAPEYFLLALLGITASAS
                     LGTDSPTKALLAALLGLMIAMVGVDPIQGTPRLTFGQQALIEGIDFLPVAIGIFGIAE
                     VLASLERAEQVAPMRTRLRDMWLSAKDWAESRVAIARGGAIGFVIGVMPGAGPTIASM
                     LAYVAEKRLSKRPERFGRGAIDGVAAPEAANNAASHAACVPMLALGIPGSASTAVLLA
                     ALILHGIRPGPGLMTEQSTLVWALIASMFIGNVILLVLNLPLAPLFAALLRVKYAYLA
                     PAILAISLVGAYANTLGFTNVWIALAFGVVGWFMIKLDFPRAPLVLALVLAPLLESSL
                     RQSLLLSFGSLKIFVERPLAAALLACVALSLAAPLYRAARRMG"
     gene            complement(1619..2068)
                     /locus_tag="VFX94_02395"
     CDS             complement(1619..2068)
                     /locus_tag="VFX94_02395"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX5767058.1"
                     /translation="MKASDVVPAALMLALCGAIALDTRRLSFWDDTTPGPAFLPVWLA
                     IAGVVLFALRLAEARRLRATMVDWPSRAALKRVAMTFAALLVVPLLAPLLGLVPALVL
                     LVAFILVVVLRQALWPSLMTLAVTTGLIYAIFVLWLRVPLPQGVLGI"
     gene            complement(2072..2974)
                     /locus_tag="VFX94_02400"
     CDS             complement(2072..2974)
                     /locus_tag="VFX94_02400"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5767059.1"
                     /translation="MKAPKGPVEITVGSGPGGTPDVIMRNIVKIMTEQKIVTIPVVVQ
                     NRPGGSHSNAYNYVLGKKGDENLLLTLASPVFTTPIMQGTPSVISQTVPIAGFIQSEL
                     VLLVQPNSPYKTLKDLVEAAKKEPKKLRVAGAASGGTDHLATAQVEKAAGIRLTYIPY
                     ESGSKALASFLGGNVEITFATLEEGLPVFQGAKARPLAILSEKRRSEPSYKDVPTAKE
                     LGYDVVFGQFWGVAAPPGTDPAIARWWEDKFRKAVATKEWQDGLKEKFQRSDFYGLEK
                     AGEYFKREEAKFRAVMEDVGLAKK"
BASE COUNT          543 a         1027 c         1034 g          479 t
ORIGIN      
        1 tgtgggttcg agtcccgccg ggcaggccag cattggttta atttgtgtca gcgttgtagc
       61 gctacggtga ccgtagctca aaggtagagt cccggattgt gattccggtt gttgtgggtt
      121 cgagtcccat cggtcacccc atccttcgcg ccgctcggta gagcggcgcc gccaacgaca
      181 gcgccacgca ggcgagcagc gccgcggcga gcggacgctc gacgaagatc ttcaggctgc
      241 cgaacgaaag cagcagcgac tgccgcagcg agctctcgag gagcggcgcg agcacgagcg
      301 cgagcacgag cggcgcgcgc gggaaatcga gcttgatcat gaaccagccg acgacgccga
      361 acgcgagcgc gatccagacg ttggtgaaac cgagtgtgtt ggcgtacgcg cccacgagcg
      421 agatggcgag gattgcgggc gcgaggtagg cgtacttgac gcgcaagagc gccgcgaaga
      481 gcggcgcgag cggaaggttc agcacgagca ggatgacatt gccgatgaac atgctggcga
      541 tgagcgccca caccagcgtg gactgttccg tcatcaggcc cggacccgga cgaatgccgt
      601 gcaggatgag cgcggcgagc agcaccgcgg tgcttgccga gccgggaatg ccgagcgcga
      661 gcatgggcac gcacgcggcg tgcgaggccg cgttgttcgc cgcctccggc gcggccacgc
      721 cgtcgatcgc gccgcggccg aagcgctcgg gacgcttgct cagacgcttc tccgcgacgt
      781 acgcgagcat ggaagcgatg gtcggcccgg cgccgggcat gacgccgatc acgaagccga
      841 tcgcgccgcc gcgcgcgatg gcgacgcggc tctcggccca gtccttggcg ctgagccaca
      901 tgtcgcgcaa gcgcgtgcgc atcggcgcga cctgctcggc gcgctcgagc gacgccagca
      961 cttccgcgat cccgaagatg ccgatggcaa ccggcaggaa atcgatgccc tcgatcagcg
     1021 cctgctggcc gaacgtcagt ctcggcgtgc cctgaatcgg atcgaccccc accatggcaa
     1081 tcatcagtcc gagcagcgcc gccagcagcg ccttggtcgg tgaatcggtg ccgaggctcg
     1141 cgctcgcggt gatgccgagc agcgcaagca ggaagtactc cggtgcattg aacttgaggg
     1201 cgaacgcggc gagcggcggg gtgacgaaaa ccactgcgac caccgccgcg atgcccgcaa
     1261 tgaacgagcc gatggcggcg atgctcaggg ctgcgcccgc gcgcccctgg cgggccatct
     1321 gatgcccgtc gatcgtagtc atcacgctcg cggcctcgcc cggcacgttg acgagcaccg
     1381 aggtgagcgt gcctccgtac tgaccgccat acatgattcc cgcgagcatc atgatcgccg
     1441 tgaccggcgg cacgccgtag gtgagcggca gcagcagcgc cattcccgcc gccggtccga
     1501 tgccgggcag ggcgccgatg atctgcccga tcagcacgcc gatcaggatg aacgacagat
     1561 tgagcggcgt gaagcacgtg gcgaagccct gtgcaaggag ctggagcggt tccatgccct
     1621 atattcccag gactccctgg gggagcggca ccctgagcca gagcacgaag atcgcgtaga
     1681 tgaggcccgt cgtgaccgct aacgtcatca ggctcggcca caacgcctgc cggagcacga
     1741 cgacaaggat gaacgccacc agcagcacga gggcgggtac gagacccagg agcggcgcga
     1801 gcagtggcac gacgagtaac gcggcgaacg tcatcgcgac ccgcttgagt gccgcccggc
     1861 tgggccagtc gaccatcgtt gcgcgcaatc gccgcgcttc cgcgaggcgc agcgcaaaga
     1921 gcaccacgcc ggcgatcgcg agccagacgg gcaggaacgc ggggccggga gtcgtgtcgt
     1981 cccagaagct caggcggcgc gtgtcgaggg cgatcgcccc gcacaacgcg agcatgagcg
     2041 ccgccggtac gacgtcgctt gccttcacgc gctacttctt ggcgagcccg acgtcctcca
     2101 tcacggcgcg gaacttcgct tcttcgcgct tgaagtactc gccggccttc tccaggccgt
     2161 agaagtcgct gcgctggaac ttctccttga ggccgtcctg ccactccttg gtggccacgg
     2221 ccttgcggaa cttgtcctcc caccagcgcg cgatcgccgg atcggttccc ggcggcgcgg
     2281 caacgcccca gaactggccg aagaccacgt cgtaaccgag ctccttcgcc gtcggcacgt
     2341 ccttgtacga cggctcggac ctgcgcttct ccgacaggat ggcgagcggc cgcgccttgg
     2401 cgccctggaa caccggcagc ccttcttcaa gagtggcaaa ggtgatctcg acgttaccgc
     2461 cgaggaacga ggcgagcgcc ttggacccgc tctcgtaggg tatgtaggtg agccggatac
     2521 ccgccgcctt ctcgacctgt gcggtggcga gatggtccgt accgccggag gccgcgccag
     2581 cgacgcgcag cttcttcggc tctttcttcg ccgcctcgac gaggtccttc agcgtcttgt
     2641 acggcgaatt cggctgcacc agcaggacca gctctgactg gatgaaaccg gcgatcggca
     2701 cggtctgcga gatcaccgac ggcgtgccct gcatgatggg cgtcgtgaaa accggcgaag
     2761 cgagcgtgag cagcaggttt tcgtcgcctt tcttgcccag cacatagttg taggcattcg
     2821 aatgcgaccc gccgggacgg ttctgcacca ccaccgggat cgtgacgatc ttctgctccg
     2881 tcatgatctt cacgatgttg cgcatgatca cgtccggcgt gccgccgggc ccggagccga
     2941 ccgtgatttc caccggccct ttgggggcct tcatctgcgc gtgaaccgcc ggggcagtca
     3001 cgatcgcgct gagcgcgcag gccgctaacg aacgcagtag cattgaggtc tcctcctttt
     3061 gtgattttcc ccagcctacg gcc
//
LOCUS       DASUNT010000120         3127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_279981, whole genome shotgun sequence.
ACCESSION   DASUNT010000120 DASUNT010000000
VERSION     DASUNT010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279981"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /gene="mraY"
                     /locus_tag="VFX94_02405"
     CDS             complement(<1..755)
                     /gene="mraY"
                     /locus_tag="VFX94_02405"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX5767060.1"
                     /translation="MLLELAQLLAKDVRLFNVFNYITLRAVLACLTALAVSLILGPYV
                     IRKLTAYKIGQAVRDDGPRSHLTKAGTPTMGGALILVSMCLTTLLWGDLSNRFVWVVL
                     LVTLAFGTIGWIDDYRKVVHRNPRGLPARWKFFWQALIGLSAALYLGTLTQAIPALNE
                     LIVPFFKHIAYPLGAAGFVVLTFFVIVGTSNAVNLTDGLDGLAIMPAVLVAGALGVFA
                     YASGNVVFSNYLGIPYIAGAGEVLVICAAIFGAG"
     gene            complement(755..2101)
                     /gene="murF"
                     /locus_tag="VFX94_02410"
     CDS             complement(755..2101)
                     /gene="murF"
                     /locus_tag="VFX94_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX5767061.1"
                     /translation="MMQLAEAAAALGGRTSGADVLFTGVSTDSRSLRARDLFVALRGE
                     RFDGHDFLKPAAEASAAAAMVDAAYRGEYPLPVLVVRDTRRSLGDLARYWRARFAPSL
                     VAIAGSNGKTTVKEMLAAVFRRHAGEAAVLATRGNLNNDIGLPLTLLALRETHRWCAI
                     ELGMNHKGEIAYLAEIARPTVALVNNAQREHLEFMRSVEEVAEENASVYDALPADGVA
                     VINADDPHAEFFRSRAGKRRTVGFGLQGAAVAARYRLQPLSSDIRLHTPAGEAAATLA
                     IPGIHNVRNALAAAACAYAVGISPMTIAEGLSAFRAYTGRLQVKKAKGGATVIDDSYN
                     ANPDSVRAAIDVLASCAGPTALVLGDMGEVGEQAAEFHREVGAYARAKGISQLLALGE
                     ATRHAVAAFGTGGQHFQRFEELVSNVRGGTVLVKGSRFMKMERVVAALTGAAEAGH"
     gene            complement(2098..>3127)
                     /locus_tag="VFX94_02415"
     CDS             complement(2098..>3127)
                     /locus_tag="VFX94_02415"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX5767062.1"
                     /translation="AVIGTLGAGFPGALSPAGNTTPDAVETQFLLKRFLERGAQAVSM
                     EVSSHGLVQGRVSGVRFSCALFTNLSHDHLDYHGSMANYAAAKARLFEMRGLEAAVLN
                     LDDALGVELGRRLAGRVRTIGYSLSPRDVPVDKALVAGRDVDPASLPVLGGFNVANAL
                     GVLGCLVAKGMTVEEGASRLANLPPVPGRMQRVGAEPLVVIDYAHTPDALQNVLEALR
                     PVAQARGGRLAVVFGAGGDRDPSKRSPMGAIASRLADRVVVTSDNPRSEDPLAIIREI
                     ENGIHGRHEAEADRARAIDSVISSSSKEDVILIAGKGHETVQEIAGRRLHFSDAEHAR
                     AALARRAG"
BASE COUNT          494 a         1062 c         1093 g          478 t
ORIGIN      
        1 cccgcgccga agatcgcggc gcagatcacc agcacctcgc cggcgccggc gatgtaggga
       61 atgccgaggt agttcgagaa cacgacgttg cccgaggcat acgcgaacac gccgagcgcg
      121 ccggcgacga ggaccgccgg catgatcgcg agcccgtcca ggccgtcggt gaggtttacc
      181 gcgttcgacg tgccgacgat gacgaagaag gtgaggacca cgaaaccggc agcgccgagc
      241 ggataggcaa tgtgcttgaa aaaggggacg atgagctcgt tcagcgccgg gattgcctgc
      301 gtcagggtcc ccaggtagag cgcggcggaa aggccgatca gcgcctgcca gaagaacttc
      361 cagcgcgccg gcagcccgcg cggattgcgg tgcacgacct tgcggtagtc gtcgatccag
      421 ccgatcgtgc cgaaggcgag cgtcaccagg agcaccaccc atacgaagcg gtttgacaga
      481 tcgccccaca gcagcgtcgt gaggcacatc gagacgagga tcagcgcgcc gcccatggtc
      541 ggcgtgccgg ccttggtgag atgcgagcgc ggcccgtcgt cgcgcaccgc ctggccgatc
      601 ttgtacgcgg tgagcttgcg gatgacatac ggaccgagga tgagcgatac cgcgagtgcc
      661 gtgagacacg cgagcaccgc gcgcagcgtg atgtagttga agacgttgaa gagccgcaca
      721 tccttcgcga gcagctgcgc gagctcgagg agcatcagtg ccccgcctcg gcggcgccgg
      781 tgagcgcggc cacgacgcgc tccatcttca tgaagcgcga tcccttgacc agcacagttc
      841 cgcccctgac gttgctcacc agttcttcga atctctgaaa atgctgccct ccggtgccga
      901 aggccgctac cgcgtgcctg gtcgcctcgc caagcgcgag aagttgcgag atgcccttcg
      961 cgcgcgcata ggcgccgacc tcgcggtgga actcggcggc ctgctcgccc acttcgccca
     1021 tgtcgccgag cacgagcgcg gtcgggcccg cgcacgaggc gagcacgtcg atcgcggcgc
     1081 gtaccgagtc ggggttggcg ttgtagctgt cgtcgatcac ggtggcgccg cccttcgcct
     1141 tcttcacctg gagccgcccg gtgtaggcac gaaaggcgct caatccttct gcgatcgtca
     1201 tggggcttat tcccacggcg taggcgcagg ccgcggcggc aagcgcgttg cgcacgttgt
     1261 ggatgcccgg aatagcgagg gtggcggcgg cttcgccggc gggcgtgtgc aggcggatgt
     1321 cactggagag cggctgcagc cggtagcgtg ctgccaccgc cgcgccctgc aggccgaacc
     1381 cgaccgtgcg acgcttgcct gcgcggctgc ggaagaactc ggcgtgcgga tcgtcggcgt
     1441 tgattaccgc gaccccgtcc gccggcagcg cgtcgtaaac ggacgcgttc tcctcggcga
     1501 cctcttcgac ggagcgcatg aactcgaggt gctcgcgctg cgcgttgttg accagcgcca
     1561 ccgtcgggcg cgcgatttcg gcaaggtacg cgatctcgcc cttgtggttc atgccaagct
     1621 cgatcgcgca ccagcggtgc gtctcccgca gggcgaggag cgtgagcggc aggccgatgt
     1681 cgttattgag attgccgcgc gtggctagca ccgccgcttc gccggcgtgg cgcctgaaga
     1741 ccgccgcgag catctccttc acggtcgtct tgccgttcga gccggcaatg gcgaccaggc
     1801 tgggcgcgaa gcgcgctcgc cagtagcgcg cgagatcgcc gagcgagcga cgcgtgtcgc
     1861 gcaccacgag cacgggcagc gggtattcgc cgcggtacgc cgcgtccacc atcgcggccg
     1921 cggcgctcgc ctcggccgca ggcttgagga agtcgtggcc gtcgaagcgt tcgccgcgca
     1981 gcgcgacgaa aaggtctcgt gcgcgcaggc tgcggctgtc ggtcgacacg ccggtgaaga
     2041 gcacatcggc gccgctcgtg cgcccgccga gcgcggcggc agcctcggcg agctgcatca
     2101 tccggccctc cgtgcaagcg cggcgcgcgc gtgctctgcg tcggagaagt gcaggcgccg
     2161 gccggcgatt tcctggaccg tctcgtggcc cttcccggcg atcagtatta cgtcttcctt
     2221 gctcgaagac gagatgacgc tgtcgatggc gcgcgcacga tcggcttcgg cctcgtgccg
     2281 gccgtggatg ccgttttcga tttcgcggat gatcgcgagc gggtcttcgc tgcgcgggtt
     2341 gtcggaggtc acgaccacgc ggtcggcgag gcgcgaggcg atcgcgccca tcggggagcg
     2401 cttgctcggg tcgcgatcgc cgccggcgcc gaacaccacc gccagccgcc cgccgcgcgc
     2461 ctgcgccacg ggacgcagcg cttcgagcac attctggagc gcatcgggag tatgcgcgta
     2521 gtcgatcact accagcggct cggcgcctac gcgctgcatc cgcccgggca cgggcggcag
     2581 gttggcgagc cggcttgccc cttcctcgac ggtcattcct ttcgcgacga gacagccgag
     2641 cacgccgagg gcgttcgcga cgttgaagcc tccgagcacc ggcagtgagg ccggatcgac
     2701 gtcccgcccg gccacgagcg ctttgtccac cggcacgtcg cgcggcgaaa ggctgtagcc
     2761 gatggtgcgc acgcgcccgg cgaggcgccg cccgagctcc acgccgagcg cgtcgtcgag
     2821 gttaagcacc gccgcctcga gcccgcgcat ctcgaagagc cgcgctttcg cggcggcata
     2881 gttcgccatc gagccgtggt agtcgaggtg gtcgtgcgaa agattggtaa agagcgcgca
     2941 cgaaaagcgc acgccgctca ctcggccttg aacgaggcca tgcgaggaca cttccatcga
     3001 aaccgcctgc gcgcctcgct ccagaaagcg cttgagcagg aattgcgtct cgactgcgtc
     3061 cggcgtcgta ttgcccgccg gtgacagcgc gcccggaaag cctgcgccca gcgtgccgat
     3121 gaccgcc
//
LOCUS       DASUNT010000121         5142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_542770, whole genome shotgun sequence.
ACCESSION   DASUNT010000121 DASUNT010000000
VERSION     DASUNT010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542770"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VFX94_02420"
     CDS             <1..420
                     /locus_tag="VFX94_02420"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HEX5767063.1"
                     /translation="FLRMLGFDTEHRNALEDAEIRRLASEDARIVLTRDRELLKCRDI
                     RRGCYVRALKPEAQLQEVVTRYGLAPVARPFTLCLHCNLGLESLAPDAVAYRVPEKVR
                     LLHRVFTHCPGCDRVYWPGSHYERMRQRLRALDSLRP"
     gene            complement(450..824)
                     /locus_tag="VFX94_02425"
     CDS             complement(450..824)
                     /locus_tag="VFX94_02425"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX5767064.1"
                     /translation="MTQPQAEFLDLYRAGLKSAADLVKASLQNAEKLQNQQLVAIRTA
                     LDQQTKTINELGQARSVDELLALQTRMAGAQWERAVGYWSELCQAQMAQARDWLSEAA
                     HAATTANAMVRAPAAKQQRRAA"
     gene            930..1181
                     /locus_tag="VFX94_02430"
     CDS             930..1181
                     /locus_tag="VFX94_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767065.1"
                     /translation="MKRIKLLIGPSLMENIVRLADYRERRQPARPASPATSPAAGAQY
                     FCTRCDGAEFLLSPLGMVHCAHCGALMRNLFVGPSLSPP"
     gene            complement(1178..1930)
                     /locus_tag="VFX94_02435"
     CDS             complement(1178..1930)
                     /locus_tag="VFX94_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX5767066.1"
                     /translation="MINFVLGLVILVIILLALSIKILREYERAVVFFLGRFQRVFGPG
                     LIVIIPGVQQAVRVDLRTRVFDVPPQDVITRDNVSVKVNAVIYFRVVDPRNAIIQVEK
                     FIEATSQLAQTTLRSVLGKHELDQLLSEREQLNLDIQTVLDAQTDAWGIKVSNVEIKH
                     VDLNETMVRAIARQAEAERERRAKVIHAEGELQASEKLLQAAQMLAQKTEAMQLRYLQ
                     TLTQIAGDKASTIVFPVPLDLLTPLLGAKKPA"
     gene            complement(1927..3219)
                     /locus_tag="VFX94_02440"
     CDS             complement(1927..3219)
                     /locus_tag="VFX94_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX5767067.1"
                     /translation="MDLLLLHRAAFYLLLSIIWGLAAGIEPAQPAPVLVVPLDGAVGP
                     ASADFVSRAIKRAAKDKAQLVIVRMDTPGGLDTSMRSLIKDILASPVPVATFVAPSGS
                     RAASAGTFILYASHIAAMAPGTNLGAASPVAIGGPPQQKDDKKGKQIEDTMTRKVTND
                     AVAYIRGLAEMRGRNADWAEKAVRESVSLSAKEALKLKVIEHIAADVPELLKKLGKAD
                     APVVEVEVDWRTKVLGVITNPAIAYIMIIVGIYALIFEFMNPGLVLPGVVGAICLLLA
                     LYAFHLLPVNYAGLALILLGIAFMIAEAFLPAFGSLGVGGLIAFVIGSVILLEDTDLP
                     GFEIPYGLIAGVTAASAAFLFFVIGVAVRNRKRPVVTGREYLIGAPAEALEDFDAEGW
                     ARVQGETWRVRSGAPVRRGQRLRVKSIDGLVLTVEAEA"
     gene            3199..>5142
                     /locus_tag="VFX94_02445"
     CDS             3199..>5142
                     /locus_tag="VFX94_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX5767068.1"
                     /translation="MKQEQVHGDAIVIRGARQNNLKHLNLAIPTGELLVVTGVSGSGK
                     SSLVFDTLYAEGQRRYVETFSPYARQFLDRMDKPQVDAVEGIPPAIGIDQTNPVRTSR
                     STVGTMTELNDHLKLLYARAAKLFCRGCGKPVVRDTPTSIFEELAKRGDARLVVTFPV
                     PVPKNFTDKEVEDLLQRQGYTRLHSRSKSLIEVIQDRVRFTPQDRARVCEALESALRF
                     GQGKVNIYGENEVLRFSSGLHCADCDIDYSEPTPAMFSFNSPLGACETCRGFGRVIGV
                     DFGLVVPDASLSLRQGAVRPWQTASFKECQDDLEKYAKRRRIALDKPWGDLSADEQDW
                     VLEGEPEWVSWRKSWPGVWYGVRRFFKWLESKAYKMHIRVLLSKYRAYTECVSCNGTR
                     LKPEGLLWKVNGKSIHDLVLLPASETRKFFDLLDFDDEATELLLKEIRTRLGYLCDVG
                     LGYLTLDRQSRTLSGGEVQRINLTTALGTSLVNTLFVLDEPSIGLHPRDMGRVIEVMK
                     RLRDAGNTLVVVEHDPQIMFAADRILDMGPGPGERGGEIVFFGSPDELRTQSTLTAEY
                     RSGRRVAAEPHAPLKPREFLEVLGAAEHNLKNIDVRIPLGRLVCVTGVSGSGKSTLVQ
                     DVLYRALLKSKGKPTEAPGAHRGL"
BASE COUNT          849 a         1723 c         1682 g          888 t
ORIGIN      
        1 ttcctgcgca tgctcggctt cgataccgag caccgcaatg cgctggaaga cgcggagatc
       61 cggcgcctcg cgagcgaaga cgcccgcatc gtgctgacgc gcgatcgcga gctcctgaaa
      121 tgccgcgaca tccgcagggg atgctacgtg cgcgcgctca agcccgaagc gcagctgcag
      181 gaggtggtga cgcgttacgg ccttgcgccg gtggcgcggc cgttcacgct gtgcctacac
      241 tgcaacctcg ggctcgagtc gctcgctccc gacgcggtgg cttaccgcgt gcccgaaaaa
      301 gtgcgcttgc tgcaccgggt tttcacacac tgcccggggt gcgaccgggt gtactggccc
      361 ggcagccact acgagcggat gcggcagcgc ctgcgggcgc tcgattcttt gcgtccctga
      421 atcgcctata gggctccgtc cctgccttct taagccgctc tgcgctgctg cttggcggcc
      481 ggcgcgcgca ccattgcgtt tgccgtcgtc gcggcatgcg cggcctcgct cagccaatcg
      541 cgtgcctgcg ccatctgcgc ctggcagagc tcgctccagt agcccacggc gcgttcccac
      601 tgggcgccgg ccatacgcgt ctggagcgcg agaagctcgt cgacgctgcg cgcctggccg
      661 agctcgttga tcgtcttggt ctgctggtcg agcgcggtgc ggatcgccac cagctgctgg
      721 ttctgcagct tttcggcgtt ttgcagcgag gctttcacaa ggtcggcggc ggacttgagc
      781 ccggcgcggt ataggtcgag aaactcggct tgcggttggg tcatcgtgtc tcctccgtca
      841 gctacgcgat gcgaatgtcc ctcccttcct acacgccgtc gtctaccagc ggcttgaaaa
      901 gaatatacgc cgcttccgaa ccggtccgca tgaaaagaat caaattgctt attggaccgt
      961 cattgatgga aaacatcgtc cgcctcgccg attaccgcga gcgccgccag ccggcgcgcc
     1021 ccgcctcccc cgcgacttcg cctgccgccg gcgcgcaata cttctgcacg cgctgcgacg
     1081 gcgccgaatt cctgctgagc cccctgggca tggttcattg cgcgcattgc ggcgcgctga
     1141 tgcgcaacct tttcgtcggt ccttcgcttt ccccgcctta ggcgggcttc ttcgctccga
     1201 gcaatggagt aagaaggtcc aacgggacgg ggaagacgat cgtcgacgcc ttgtcgccgg
     1261 cgatctgcgt cagcgtctgc aggtagcgca gctgcatcgc ctcggtcttc tgggcgagca
     1321 tctgggcggc ctgcagcagc ttctcggagg cctgcagctc gccctcggcg tggatcacct
     1381 tggcgcggcg ctcgcgctcg gcctcggcct ggcgcgcgat cgcgcgcacc atggtctcgt
     1441 tcaggtccac gtgcttgatc tcgacgttgg acaccttgat gccccaggcg tcggtctgcg
     1501 cgtcgagcac ggtctggatg tcgaggttga gctgctcgcg ctccgagagc agctggtcga
     1561 gctcgtgctt gccgagcacc gagcgcagcg tggtctgcgc gagctggctc gtcgcctcga
     1621 tgaacttctc cacctggatg atcgcattgc gcgggtcgac cacgcggaag tagatcaccg
     1681 cgttcacctt caccgagacg ttgtcgcgcg tgatcacgtc ctgcggcggc acgtcgaaca
     1741 cgcgcgtgcg caggtcgacg cgcaccgcct gctgcacgcc cgggatgatg acgatcaggc
     1801 ccgggccgaa cacgcgctgg aagcgcccga ggaagaacac cacggcgcgc tcgtactcgc
     1861 gcaggatctt gatcgagagc gcgagcagga tgatgaccag tatgaccagc ccgagaacga
     1921 aattgatcac gcttctgcct ccactgtgag taccaagccg tcgatggatt tgacccgcag
     1981 gcgctggccg cggcgcaccg gcgcgccgga gcgaacccgc caggtctccc cctgcacgcg
     2041 cgcccagccc tcggcgtcga agtcttcgag cgcctcggcc ggcgcgccga tcaggtactc
     2101 gcggccggtg accaccgggc gcttgcggtt gcgcaccgcg acgccgatga cgaagaacag
     2161 gaacgccgcg ctcgccgcgg tcacgccggc gatcaggcca taggggatct cgaagcccgg
     2221 gaggtcggta tcctcgagaa ggatcaccga gccgatgacg aacgcaatca gcccgccgac
     2281 gccgagcgag ccgaacgcgg gcaggaacgc ctcggcgatc atgaacgcga tgccgaggag
     2341 gatgagcgca aggccggcgt agttgaccgg cagcagatgg aaggcgtaca gcgcgagcag
     2401 caggcagatg gcgccgacca cgcccggcag gacgaggccg gggttcatga actcgaaaat
     2461 cagcgcgtaa atcccgacga tgatcatgat gtaggcgatc gccgggttgg tgatcacgcc
     2521 gaggaccttg gtgcgccagt cgacctcgac ttcgaccacc ggcgcgtccg ccttgccgag
     2581 cttcttgagg agctccggga cgtcggcggc gatgtgctcg attaccttga gcttgagcgc
     2641 ctccttagcc gacaggctca ccgactcgcg cactgccttc tcggcccagt cggcgttccg
     2701 gccacgcatc tcggcgaggc ccctgatata ggcgacggcg tcgttggtga ccttccgggt
     2761 catggtgtct tcgatctgct ttcccttctt gtcgtctttc tgctgcggcg gccctccgat
     2821 cgccacgggg gacgccgccc cgaggttggt ccccggcgcc atggccgcga tatggctcgc
     2881 gtacaggatg aaggtgccgg cgctcgcggc gcgcgagccg ctcggcgcga cgaaggtggc
     2941 caccggcacc ggcgaggcga ggatgtcctt gatcaggctc cgcatcgagg tgtcgagccc
     3001 gcccggggtg tccatgcgca cgatcacgag ctgcgcctta tccttcgcgg cgcgcttgat
     3061 cgcgcggctc acgaaatccg ccgacgcggg acccacggcg ccatcgagcg gcaccacgag
     3121 caccggcgcc ggctgcgccg gctcgatccc ggcggctaga ccccaaatga tgctcagcag
     3181 caagtagaat gccgcccggt gaagcaagag caagtccacg gcgatgccat tgtcatccgc
     3241 ggcgcccgcc agaacaacct caagcacctg aacctcgcga tcccgaccgg cgagctcctc
     3301 gtggtgaccg gcgtttcggg ctcgggcaag tcatcgctcg tcttcgacac cctgtatgcc
     3361 gagggccagc ggcgctacgt cgagaccttc agcccctatg cgcgccagtt cctggaccgc
     3421 atggacaagc cgcaggtcga cgccgtcgag ggcatcccgc ccgcgatcgg gatcgaccag
     3481 accaacccgg tgcgcacctc gcgctccacg gtcggcacga tgaccgagct gaacgaccac
     3541 ctcaagctcc tgtatgcgcg cgcggcgaag cttttctgcc gcggctgcgg caagccggta
     3601 gtgcgggaca caccaacctc gatctttgaa gagctcgcga agcgcgggga tgcccgcctg
     3661 gtcgtcacct tcccggtgcc ggttccgaag aactttacgg ataaggaagt cgaagatctc
     3721 ctccagcgcc agggctacac gcggcttcat tcgcgctcga agagcctgat cgaagtcatt
     3781 caggaccgcg tgcgcttcac gccgcaagat cgggcgcgag tgtgcgaggc gctcgaatcg
     3841 gccctccggt tcggtcaggg caaagtgaat atttacgggg agaacgaagt gttgcggttc
     3901 tcctccggcc tgcattgcgc cgattgcgac atcgattact cggagccgac gccggcgatg
     3961 ttctccttca actcgccgct cggcgcgtgc gagacctgcc gcggcttcgg ccgcgtgatc
     4021 ggcgtggatt tcgggctcgt cgtgccggat gcgtcgctct ctctcaggca gggcgcggtg
     4081 cggccctggc agacggcgag cttcaaggag tgccaggacg acctggagaa gtacgcgaag
     4141 agaagacgga ttgcactcga caagccgtgg ggggatctgt ccgctgatga acaagactgg
     4201 gtgctggaag gcgagcccga gtgggtgagc tggcgcaagt cctggcccgg cgtgtggtac
     4261 ggcgtgcgcc gctttttcaa gtggctcgag agcaaggcct acaagatgca catacgggtg
     4321 ctgctttcca agtaccgggc ctacacggag tgcgtctcgt gcaacggcac gcggctcaag
     4381 cccgaggggc tgctgtggaa ggtgaacggg aaatccatcc acgacctcgt gctcttgccg
     4441 gcctcggaaa cgaggaagtt cttcgatttg ctcgatttcg atgacgaggc gaccgagctc
     4501 ctcctcaagg agatccgcac gcggctcggc tacctgtgcg acgtgggcct gggctacctc
     4561 acgctcgacc gtcagtcgcg cacgctctcc ggcggcgagg tgcagcgcat caacctcacc
     4621 actgcgctgg gcacgtcgct cgtcaacacg ttgttcgtgc tggacgagcc ctccatcggc
     4681 ctgcatccgc gcgacatggg acgcgtgatc gaagtgatga agcgcctgcg cgacgcgggc
     4741 aacacgctgg tcgtggtcga gcacgacccg caaatcatgt ttgccgccga ccgcatcctc
     4801 gacatggggc ccggcccggg ggaacgaggc ggcgagatcg tgttttttgg ttcacccgac
     4861 gaactaagaa cccaatccac gttgactgcg gaataccgct ccggccgccg cgttgccgcc
     4921 gagccgcacg cgcccctgaa gccgcgcgag ttcctcgaag tgctgggagc ggccgagcac
     4981 aacctcaaga atatcgacgt gcgcatcccg ctcggacgcc tggtgtgcgt gaccggcgtc
     5041 tcaggctcgg gcaagtccac gctggtacag gacgtgctct accgggcgct cctcaagtcg
     5101 aagggaaagc ccaccgaggc gccgggcgcg caccgcggcc tg
//
LOCUS       DASUNT010000122         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_805977, whole genome shotgun sequence.
ACCESSION   DASUNT010000122 DASUNT010000000
VERSION     DASUNT010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805977"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFX94_02450"
     CDS             <1..363
                     /locus_tag="VFX94_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767069.1"
                     /translation="RHWDMLDIDEQGRVGSIDLKPQSSPLRFSWTCAVWSPAFSRFMH
                     DCVERERATHARNALAFRGIDAQGDLPVGAIIRAAIEKGLRVHGATFPDETCIDIGTP
                     ENLIRALRTRLERRQGQS"
     gene            360..899
                     /locus_tag="VFX94_02455"
     CDS             360..899
                     /locus_tag="VFX94_02455"
                     /inference="COORDINATES: protein motif:HMM:NF019440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEX5767070.1"
                     /translation="MSGAIHSLWFKPTGPAFNVLADVVDRLARELGASPFEPHVTLLG
                     NLEGSQREILWRSEALARKLRRFEIELTEPAYGQEHFQCVFMRVKETPSVMGANSRAR
                     PVFGQAPGGYLPHLSLVYGSFPDARKREIIARLPDLRLSFEAAAVHVIRADTDDPKNW
                     HELAAFPIEDDRVAMARRS"
     gene            920..1564
                     /locus_tag="VFX94_02460"
     CDS             920..1564
                     /locus_tag="VFX94_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5767071.1"
                     /translation="MRDAVTPSKHLVEEIESAILGAVHPGLPARDVAELIVLAKARPL
                     SFASAGNGSPQHLCGELLKLSAGIDLAHIPYKGAAPAQQDVVAGQVSLICNALSVMLP
                     PVRAGRLRAIALTAKSRHPQASDIPTAHEAGLRGFEFEVWFGFVAPSATPDPILERLN
                     AEFGRALRAPEIAARLEALGLTVVAGSPREFDAFIAAEAAKWRGIVERAWVKAD"
     gene            1730..1986
                     /locus_tag="VFX94_02465"
                     /pseudo
     CDS             1730..1986
                     /locus_tag="VFX94_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453978.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
BASE COUNT          369 a          706 c          696 g          373 t
ORIGIN      
        1 aggcattggg acatgctcga cattgacgaa caaggacgtg tgggttccat cgatctcaag
       61 cctcagtcgt cgcccttgcg tttctcatgg acctgcgccg tatggtcgcc cgctttcagc
      121 cggttcatgc acgactgtgt cgaacgcgaa cgcgcaaccc atgctcgcaa cgcgcttgcg
      181 tttcgcggca tcgatgccca aggcgatctg ccggtcggcg cgatcatccg cgcggccatc
      241 gagaaaggcc tgcgcgttca cggcgcgacg ttccctgacg agacgtgcat cgacatcgga
      301 acgcctgaaa acctgatacg cgcgctgagg acaagactcg agaggcgcca gggccagtca
      361 tgagcggcgc cattcattcc ctgtggttca agccgacggg accggctttc aacgtcttgg
      421 ctgacgtcgt cgacaggctc gcgcgcgagc tcggcgcttc gccattcgag cctcatgtca
      481 ccctgctcgg aaacctggag ggatcccaac gggagattct ttggcgaagc gaagccctcg
      541 cccggaaact tcgccggttc gagatcgagc ttaccgagcc ggcttacggg caggaacact
      601 tccagtgcgt gttcatgcgc gtgaaggaaa cgccgtcggt catgggggca aattcacggg
      661 cgaggccggt gttcggtcag gcgcccggcg ggtacttgcc tcatctgagt ctggtctacg
      721 gctcgtttcc agacgcccgg aaacgcgaaa tcatcgcccg gctacccgac cttcgactct
      781 cgtttgaggc ggctgctgtc cacgtgatca gagcggacac cgacgatccg aagaactggc
      841 acgagctcgc cgcttttcca atcgaggatg accgcgtcgc catggctcgc cggtcctgat
      901 caactgaatc gactggccga tgcgcgatgc agtgactccg tcaaagcacc tcgttgaaga
      961 gatagagagc gctatcctgg gcgcggtgca tccgggcctc ccggcgcgcg acgtcgcgga
     1021 gctgattgtg ctcgccaagg caaggcccct cagcttcgcg tcggcgggaa acggcagtcc
     1081 ccagcacctc tgcggcgagc tgctgaagct ttcggccggc atcgatctcg cgcacatccc
     1141 atataagggc gcggcgccgg cgcagcagga tgtggtggcg ggacaggtgt cgctgatctg
     1201 caatgcgctc tccgtcatgc ttccgccggt gcgtgcgggc cgcttgcgcg ccatcgcgct
     1261 caccgccaag agccgtcatc ctcaggcgag cgacatcccg accgcgcacg aagcggggtt
     1321 gcggggattc gagttcgagg tgtggttcgg gttcgtcgcg ccctcggcga cgcccgaccc
     1381 gatcctggag cgactgaatg ccgagttcgg ccgtgcgctg cgcgcgccgg agatcgccgc
     1441 tcggctcgag gccctcggcc tcaccgtcgt cgcaggctct ccgcgcgagt tcgacgcgtt
     1501 catcgccgcc gaggccgcga aatggcgcgg catcgtcgag cgcgcctggg tgaaggccga
     1561 ctagcgctgt agcggccccg gacgggccac ggcgacaagc ttcgtcttgc cgaaacttgc
     1621 atagaatgcc cggctcgaat cactttttcg ggtaaggcga cctatcgcgg cagcgcaagg
     1681 caaagtcatc atcacgtgcg cggtgacggg cgcgatccac acgccgtcga tgtcgccgca
     1741 gcctcccatc acgcccgacg agatcgccga ggcggcgctc ggcgcggcgc aggccggcgc
     1801 ggcgatcgtg cacctgcacg cgcgcaatcc caaggacggc agccccacgc aggacccgga
     1861 gctctttcgc cagttcggcg cgaagatccg cgccgcgagc gacgtggtgc tcaactcacc
     1921 accggcggcg cgcccaccat gagcatcgaa gagcggctgc agccggcgct caagctgaag
     1981 cccgagcgcc tcgggttggg caaagcgggg tttagctggc gtccccacgg ggagactcgg
     2041 acacctcgga cacgccctcg gattcattgt ttttccgagg tcttttcgga gctggagcgg
     2101 cctcggtgta acagcgtggt ttgacaccga ttgcaaggag ctta
//
LOCUS       DASUNT010000123         2830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_104720, whole genome shotgun sequence.
ACCESSION   DASUNT010000123 DASUNT010000000
VERSION     DASUNT010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104720"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            364..993
                     /locus_tag="VFX94_02470"
     CDS             364..993
                     /locus_tag="VFX94_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5767072.1"
                     /translation="MRILIADDHPIVRHGLKQMLAADPSATIVGEATNGDEALALARK
                     LEWDMAIFDFTMPGRSGLDLLGDMKKEFPGRPVLILSMHSEDVHGSRVLRGGAAGYIT
                     KDSAPQELATAVRKVANGGKYVSASLAEILASECSSDPQKALHEKLSDREYRVMWLLA
                     TGKQISEIAKEMGLSPSTVSTYRTRILRKLGLTSNAALVHYAVRHQLVG"
     gene            990..2108
                     /locus_tag="VFX94_02475"
     CDS             990..2108
                     /locus_tag="VFX94_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5767073.1"
                     /translation="MNDVANIQDLEAEPRAAEEQALEKAKILLVDDEPKSLFALQELL
                     SMLGQNLMVAQSGEEALRLALKNDFAVILLDVRMPGIDGFETARLIRARDRSKSTPII
                     FLTAAADEMTSMFRGYEVGAVDYLMKPVVPEVLKSKVAVFVELFRKSERLRESEDKLR
                     RLAAHLISVREEERAHIAREIHDELGQVLTGLKMEVTWLAKRLKDKPLIEKTDSMCAL
                     IDSSVQTVRKIATGLRPEMLDDMGLIAAVAWQAKEFQKRTGIRCRAKLPAEVKFDIDI
                     STTMFRIFQEILTNVARHSRATRVDIELTLGAEQIGLEVSDNGVGIQDNELHARKSLG
                     LLGMQERALLFGGDVRISGSPGHGTRVSVTIPLSRKPK"
     gene            2105..2734
                     /locus_tag="VFX94_02480"
     CDS             2105..2734
                     /locus_tag="VFX94_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5767074.1"
                     /translation="MKVLIADDHPVVRQGLRQMLAVESDLTVVGEARNGQEVVELSRR
                     VPWEVAVLDYNMPGKNGLELIKELRQRYPGRAVLVLSMYPEDRYAVRALKAGAAGYLT
                     KESAPEELVSAIRKVAGGGRYVTPTLGEKLALELEDNRGKPLHETLSDREYQVMWMIA
                     SGKTVRQIADELFLSPNTVSTHRTRILRKMNMKSNAELMHYAIAHHLVD"
BASE COUNT          556 a          917 c          914 g          443 t
ORIGIN      
        1 tccctttgtt ttggggaaat ggactacgtg gccctggctt gagcacgagc cgtgccatcg
       61 gatccactac aaggggaatc aacgggttaa ctacaaaacg tagggcgaag cactacaggt
      121 ctgtagcgct gcgacgacgc cgcgcgctat gaaatcctca agtccctgtt tcggcacgcg
      181 atgctcatgt cgcggcgcga cgactttcgg cgcaacgacg cattgtggtt gcgtgccgcg
      241 cagcgtcggg tctctaccga cacgcgtgtc aactccttgc tacatcagaa aaattttcct
      301 ctcagagcga tggccaagcg ggccgcgcgt gcctatactt tttcaacgag ggagaaacac
      361 cacatgcgaa ttctgattgc agacgaccac ccgatcgtgc gccacggcct caagcagatg
      421 ctggcggccg acccatcggc aaccatcgta ggtgaagcga ctaacggcga cgaggcgctc
      481 gcgctcgcgc gcaagctcga atgggacatg gcgatcttcg atttcaccat gcccgggcgc
      541 agcggcctgg acctgctcgg cgacatgaag aaggagttcc ccggccgccc ggtgctgatc
      601 ctcagcatgc actccgagga cgtgcacggc tcgcgcgtgc tgcgcggcgg cgcggcgggc
      661 tacatcacca aggacagcgc gccgcaggag ctggcgaccg cggtgcgcaa ggtggcgaac
      721 ggcggcaagt acgtctcggc ctcgctggcc gagatcctcg cctccgaatg ctcgagcgat
      781 ccgcagaagg cgctgcacga gaagctctcc gaccgcgagt accgcgtgat gtggctgctc
      841 gccaccggca agcagatcag cgagatcgcg aaggagatgg gcttgagccc gtcgacggtg
      901 agcacctatc gcacgcgcat cctgcgcaag ctcggcctga cgagcaacgc agcgctggtg
      961 cattacgcgg taagacatca gctggtcggg tgaacgacgt agccaacatt caggatctgg
     1021 aagccgagcc gcgcgccgcc gaggagcagg cgctcgagaa ggcgaagatc ctgctcgtcg
     1081 acgacgagcc gaagagcctg ttcgcgctgc aggagctgct gtcgatgctc gggcagaacc
     1141 tgatggtggc gcagtcgggc gaggaggcgc tgcgcctcgc gctcaagaac gacttcgcgg
     1201 tcatcctgct cgacgtgcgc atgccgggaa tcgacggctt cgagaccgcg cgcctgatcc
     1261 gcgcgcgcga ccgctcgaag tccacaccca tcatcttcct caccgccgcg gccgacgaga
     1321 tgacctcgat gttccgcggc tacgaggtcg gcgcggtcga ctacctgatg aagccggtgg
     1381 tgcccgaggt gctgaagtcg aaggtggcgg tgttcgtcga gctgttccgc aagagcgagc
     1441 ggctgcgcga gtcggaagac aagctgcgcc gcctggcggc gcacctgatc agcgtgcgag
     1501 aggaagaacg cgcgcacatc gcgcgcgaaa tccacgacga gctcggccag gtgctcaccg
     1561 gcctgaagat ggaagtcacc tggctcgcca agcgcctgaa ggacaagccg ctcatcgaga
     1621 agaccgactc gatgtgcgcg ctgatcgatt cgtcggtgca gacggtgcgc aagatcgcca
     1681 ccggcctgcg tcccgagatg ctcgacgaca tgggcctgat cgccgcggtg gcgtggcagg
     1741 ccaaggaatt ccagaagcgc accggcatcc gctgccgggc caagctgccg gccgaggtga
     1801 agttcgacat cgacatctcg accaccatgt tccgcatctt ccaggagatc ctcaccaacg
     1861 tggcgcgcca ttcgcgcgcc acgcgcgtcg acatcgagct cacgctcggc gccgagcaga
     1921 tcggcctgga ggtgagcgac aacggcgtcg gcatccagga caacgagctg cacgcgcgca
     1981 agtcgctcgg gctgctcggc atgcaggaac gcgcgctgct cttcggcggc gacgtgcgca
     2041 tcagcggctc tcctgggcat ggcaccaggg tctcggtgac catccctctt agcagaaagc
     2101 ccaaatgaaa gttctcatcg ctgacgatca cccggtagtg cgccagggcc tgcggcagat
     2161 gctcgccgtc gagtccgacc tgacggtggt cggcgaggcg cgcaacggcc aggaagtggt
     2221 cgagctctcg cggcgcgtgc cgtgggaagt ggcggtgctc gactacaaca tgcccggcaa
     2281 gaacggcctc gagctcatca aggagcttcg ccagcgctat cccggccggg cggtgctcgt
     2341 cttatcgatg tatccggaag atcgctacgc ggtgcgcgcg ctcaaggcgg gcgcggcggg
     2401 atacctcacc aaggaaagcg cgcccgagga gctggtcagc gcgatccgca aggtcgccgg
     2461 cggcggccgc tacgtcacgc ccacgctcgg cgagaagctc gcgctcgaac tcgaggacaa
     2521 ccgcggcaag ccgctccacg agacgctctc cgaccgcgag taccaggtga tgtggatgat
     2581 cgcctcgggc aagaccgtgc gccagatcgc ggacgagctc ttcctcagcc cgaacaccgt
     2641 cagcacccac cgcacgcgca tcctgcgcaa gatgaacatg aaatccaacg cggagctgat
     2701 gcactacgcc atcgcccacc acctggtgga ctaaggaaat cggggtctga ccccgatttt
     2761 ctctgtagta gaacgactac aaaagcgcgg gagatccagc gatatccccg ctcccgtttc
     2821 ctttctacga
//
LOCUS       DASUNT010000124         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_16703, whole genome shotgun sequence.
ACCESSION   DASUNT010000124 DASUNT010000000
VERSION     DASUNT010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16703"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VFX94_02485"
     CDS             <1..716
                     /locus_tag="VFX94_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5767075.1"
                     /translation="GMAFALRAYLAFALIAPFVFAAAATRNIAWAWKVGGVMTRFFLR
                     LSGLTVLVRGRESIPPGPAVLALNHTSYLDALVLVSVLAPRPYAFIAKREFLGNPAMR
                     LLLGGFGCLFVERFDVQKSAEHADELVVAARSGRSLAIFPEGTLQRRPGLMPFRTGAF
                     QVAVQAGVPVVPVALRGVRSVLRDGTWFARRHPMAAIFGAPIFPEGDDWNAAVRLRGR
                     VRAEILKACGEPDLETQPV"
     gene            complement(713..1549)
                     /locus_tag="VFX94_02490"
     CDS             complement(713..1549)
                     /locus_tag="VFX94_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX5767076.1"
                     /translation="MKLKQVDVFTDKPFFGNPVAVVLGAETLDAQAMQRIACWTNLSE
                     TTFLLKSSTADYRLRIFTPRQELPFAGHPTIGSAHAALESGFVGPKKKLLQECGAGLI
                     ELSVEDDGRIFLKGPAAKPQRLERQIPSIPLAPASAVMKVDVGPVWVVAEVGDAVALA
                     ALKPDMTALAEWSDALHVTGVTVFAPSGEAQSQIHVRSFAPAHGIPEDPVCGSGNLSV
                     GVFLRETKQLDRFGKAYVARQGMQLGRDGKVAVRVHADHIEIGGHAVTCVDGEIGRQG
                     RV"
     gene            complement(1546..>2135)
                     /locus_tag="VFX94_02495"
     CDS             complement(1546..>2135)
                     /locus_tag="VFX94_02495"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX5767077.1"
                     /translation="SVGVIGLGAGVIAAWMKPGDHLVFYEISPRVVDVAQREFTFLRD
                     TLAKSEVVLGDGRLSLEREPPRGYDVLGIDAFSGDSIPMHLVTREAMAIYLKHLKPDG
                     VIVFQATNRFIDLLPVVKRLATEFGLEAVNVADEPEGGEGPEYWYSSTDQVIVTRNRK
                     LLSAPRIAEVAAQIEDRPELPTFTDAHHNLLRILK"
BASE COUNT          321 a          728 c          724 g          362 t
ORIGIN      
        1 gcggcatggc gttcgccctg cgtgcgtacc tcgctttcgc gctcatcgcg ccttttgtct
       61 tcgccgccgc ggccacgcgc aacatcgcgt gggcgtggaa ggtgggcggc gtgatgacgc
      121 ggttcttcct tcgcctctcc ggcctgacgg tcctcgtgcg cggacgcgag agcatcccgc
      181 ccggaccggc ggtgctcgcg ttgaaccata cgagctatct cgatgcgctc gtcctcgtct
      241 cggtgctcgc gccccgcccg tacgcattca tcgccaagcg cgagttcctc ggaaaccccg
      301 cgatgcggct attgctcggc gggtttggct gcctgttcgt cgagcgcttc gacgtgcaga
      361 aaagcgccga gcatgccgac gagctcgtcg tggcggcaag aagcggccgc tcgctcgcca
      421 tctttcccga gggcacgctg cagcgccggc cggggctgat gccgtttcgc accggcgcgt
      481 tccaggtggc ggtgcaggcc ggcgtaccgg tcgtgccagt cgcgctgcgc ggcgtgcgct
      541 cggtgctgcg cgacggcacc tggtttgcgc gccgccaccc gatggcggcg atcttcggtg
      601 cgccgatctt tcccgagggc gatgactgga acgcggccgt gcgcctgcgc ggacgcgttc
      661 gcgccgagat actgaaagcc tgcggcgagc ccgacctcga gacccagccg gtctagacgc
      721 gaccctggcg cccgatttcc ccgtccacgc aggtgacggc gtggccgcct atctcgatgt
      781 ggtcggcgtg cacgcggacc gcgacctttc catcgcgtcc gagctgcatg ccctggcgcg
      841 cgacgtaggc tttcccgaac cggtcgagct gcttcgtttc ccgcaggaag acgccgacgc
      901 tcagattgcc gctgccgcaa accgggtcct ccgggatgcc gtgcgccggc gcgaaggagc
      961 ggacgtggat ctgcgactgc gcctcgcccg aaggcgcgaa aaccgttacc ccggtaacgt
     1021 gaagcgcatc gctccattcc gcgagcgcgg tcatgtcggg cttgagcgcg gcaagcgcca
     1081 cggcatcgcc gacttcggcg acgacccaga ctggaccgac atcgaccttc atcaccgcgc
     1141 tcgccggggc gagcggaatg gaggggatct gccgctccag gcgctgcggc ttcgccgccg
     1201 gccccttcag gaagatgcgg ccgtcgtcct cgacggaaag ctcgatcagc ccggcgccgc
     1261 actcctgcaa aagctttttc tttgggccaa cgaatccgga ctcgagcgcc gcgtgcgcgg
     1321 agccgatcgt cggatgcccg gcgaaaggca gctcctgccg cggcgtgaag atgcgcagcc
     1381 ggtagtccgc cgtggaggat ttcagcagaa aggtcgtctc ggaaagattg gtccagcagg
     1441 cgatgcgctg catggcctgc gcatcgaggg tttccgcgcc cagcaccacg gccaccgggt
     1501 tgccgaaaaa cggcttgtcg gtgaagacat cgacctgctt cagcttcact tcagtattcg
     1561 aagcaggttg tggtgcgcat cggtgaaggt cggcagctcg ggccgatcct cgatctgcgc
     1621 ggcgacctcg gcgatgcgcg gcgcgctcag cagcttgcgg ttgcgggtga cgatgacctg
     1681 gtcggtggac gaataccagt actccgggcc ctccccaccc tccggctcgt cggcgacgtt
     1741 caccgcctcg aggccgaact cggtggcgag gcgcttcacc accgggagca ggtcgatgaa
     1801 gcggttggtg gcctggaaca cgatgacgcc gtcgggcttg aggtgcttca ggtagatcgc
     1861 catcgcctcg cgcgtgacga ggtgcatcgg gatcgagtcg ccggagaagg cgtcgatgcc
     1921 cagcacgtcg tagccgcgcg gcggctcgcg ctcgagcgac aggcgcccgt cgccgagcac
     1981 gacctcgctc ttcgccaaag tgtcgcgcag gaaggtgaat tcgcgttgcg cgacgtcgac
     2041 tacgcgcggg ctgatctcgt agaaaacgag atggtcaccc ggcttcatcc aagccgcgat
     2101 cacccccgcg ccgaggccga tgacgccgac cgaaa
//
LOCUS       DASUNT010000125         3927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_806018, whole genome shotgun sequence.
ACCESSION   DASUNT010000125 DASUNT010000000
VERSION     DASUNT010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806018"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFX94_02500"
     CDS             complement(<1..373)
                     /locus_tag="VFX94_02500"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5767078.1"
                     /translation="MIAHVLDHPRWPLLVYPRAVTEPSPELFEQLFAANRWPAAWRNG
                     VYPFHHFHGNSHEVLGVYEGEVTVQFGGDAGVALTAKPGDVIILPAGTRHKKLSSRGA
                     LGIVGAYPEGSRPDTCRGAASR"
     gene            complement(376..1422)
                     /locus_tag="VFX94_02505"
     CDS             complement(376..1422)
                     /locus_tag="VFX94_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX5767079.1"
                     /translation="MKLLVLPGDGIGPEITAATVAVLQAAARRFGLDLSLEERQIGLA
                     ALAKFGTTLPDGVLERARELDGVILGPISHLEYPAPDKGGVNVSAAFRVRLDLYANVR
                     PARSRAGLGRAMDLVIMRECTEGFYPDRNMHLGSGEFMPVPGVALSVRKITAHACERI
                     ARRAFELAGKRRKLVTAVHKANVFRVSDGLYLDTVRAVAGDFPDVRLEEEIVDAMAAK
                     LARHPERYDVVLATNFYADILSDLASELSGSLGLAGSILAGEARCAAQAQHGSAPDIA
                     GKDRANPTSLILSAAMLLDWLGEKRKSENLLRAGKAIDAAVDAVLSSRETRTADLGGK
                     LGTRAFGEAVAAAL"
     gene            1437..2072
                     /locus_tag="VFX94_02510"
     CDS             1437..2072
                     /locus_tag="VFX94_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX5767080.1"
                     /translation="MRIALIHAVGLAMQPVDQAFRRHWPGVERINLLDDALSPDRDRA
                     GALTAELSARISALANYAISAGASGILYTCSAFGEAIDAVRQRVRMPVLKPNEAMFAE
                     AIAHGSRLGLLATFPASVGSMARELEALAPRAELETACVPQALAALRAGDGAQHDALL
                     AAVAPKLSHCDALMLAQFSTARAREAVARVVKCPVLTSPDSAVLAMRRALG"
     gene            2080..3003
                     /locus_tag="VFX94_02515"
     CDS             2080..3003
                     /locus_tag="VFX94_02515"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX5767081.1"
                     /translation="MCRALLYLGEPVLLDHFLYQPDSALVRQAYMPKMLNMLNLAGFG
                     LRAWDPASHDPARAYAYHSQEVPVFDRNLKNLAEKVRAGCLLAHVRGVADETAADISL
                     QNCHPFQFKGVPLALAHNGELARFAEMKSLLAAAVKPELLSQIRGTTDSEWVYALLVS
                     QLRDPHRRPGGEELARAVEDTLRVLGEARARLGIELSSSLNLFIADGTQLAAVRYCFD
                     YGCYPTDDASHLRELDLSYLSLWYTAGRDYGLHDGEWKMTGASENANSVLIASEPLTR
                     DTAAWVEVPEYSMLHVALRAGRPAISIRDLH"
     gene            complement(2981..>3927)
                     /locus_tag="VFX94_02520"
     CDS             complement(2981..>3927)
                     /locus_tag="VFX94_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5767082.1"
                     /translation="LLRYGEPSRGAYILEQGAAEATVLLPGGEKLAVATLPAGSVFGE
                     TALIERGTCTASVAAKESLEGWFIDRDDFRSLSAQRSAAALRIQHAVTLEISRKLRAL
                     NAKLMEAPAPQDAAASQPCSPADPLAGVERLKQASFDVEPFLPHLGVFEAFDPADIAA
                     LAAVAQLLELPRGHAVFFSGQASAGCFIVVRGAVEIRARHAQRSRRIAVLGPGQPLGY
                     MSALERGAHGADAIVREPAILLEIPRDAFEKLYFGGSPVSGRLHRAIQRSLLYSLGQT
                     NRHLTRLISQARLRGARNEGDQLEKAYGGQIVSGDPGY"
BASE COUNT          582 a         1396 c         1335 g          614 t
ORIGIN      
        1 ttctgctggc tgcgccgcgg caggtgtcgg gacggctgcc ctccggataa gcgcccacga
       61 tgccgagcgc gccacgcgag gaaagcttct tgtggcgcgt tcccgccggc aggatgatca
      121 cgtcaccggg cttcgcggtg agcgcaacgc ctgcgtctcc gccgaactgc accgtgacct
      181 ccccctcgta gacgccgagc acttcgtggc tgttgccgtg gaagtggtgg aagggataca
      241 cgccgttgcg ccacgccgcg ggccagcggt ttgcggcaaa cagttgctcg aacaactcgg
      301 gactgggctc ggttacggct cgcgggtaga ccaatagcgg ccagcgcgga tgatcgagga
      361 cgtgcgcgat cacgttcata gcgcggccgc aaccgcctcg ccgaacgccc gcgtgccgag
      421 ctttccgcca agatccgcgg tcctggtttc cctgctggaa agaaccgcgt ccacggcggc
      481 gtcgatcgcc ttgcccgccc gcagcaggtt ttcggatttg cgcttttcgc ccagccagtc
      541 aagaagcatc gcagccgaca ggataagcga agtgggattc gctctgtctt tccccgcgat
      601 gtcgggcgcg gagccgtgct gcgcctgcgc cgcgcagcgc gcctctccgg ccaggatcga
      661 tcccgcgagc ccgaggctgc cggagagctc ggaggcgagg tcggacagga tgtcggcgta
      721 gaagttggtg gcgagcacga cgtcgtagcg ctcggggtgg cgcgcgagct tggccgccat
      781 cgcatcgacg atttcttcct cgagcctgac gtccggaaaa tcgcccgcca ccgcgcgcac
      841 ggtgtcgagg tacagcccgt cgctcacgcg gaacacgttc gccttgtgaa ccgcggtgac
      901 cagctttctt ctcttgccgg cgagctcgaa agcccggcgc gcaatgcgct cgcacgcgtg
      961 cgcggtgatc ttgcgcaccg aaagcgccac ccccggcacc ggcatgaact ccccgctccc
     1021 caggtgcatg ttgcggtcgg ggtagaagcc ctcggtgcac tcgcgcatga tcaccaggtc
     1081 catcgcccgg ccgagccccg cgcggctgcg cgccggccgc acgttggcgt agaggtcgag
     1141 ccggacgcgg aaggccgccg acacgttcac gccgcccttg tccggcgccg ggtactcgag
     1201 gtgcgagatc gggccgagga tcacgccatc gagctcgcgc gcccgctcca ggaccccgtc
     1261 agggagcgtc gtgccgaatt tcgcgagcgc ggcaaggccg atctgccttt cttcgaggga
     1321 aagatccagc ccgaaacggc gagcggccgc ttgcaggacc gccaccgtgg ccgcggtgat
     1381 ctccgggccg atgccgtcgc cgggaagaac gagaagcttc acctatactc ctttgcatgc
     1441 gcatcgcgct gatccacgcc gtcgggctcg ccatgcagcc cgtggaccaa gctttccggc
     1501 gccactggcc cggcgtcgag cgcatcaatc tgctggacga cgcgctgtcg cccgaccggg
     1561 accgcgccgg cgcgctcacc gccgagctct cggcgcgcat ctcggcgctc gccaattacg
     1621 ccatcagcgc cggggcctcg gggattctat acacctgctc cgcgttcggc gaagcgatcg
     1681 acgcggtcag gcagcgcgtg cgcatgccgg tgctgaagcc gaacgaagcg atgttcgccg
     1741 aggccatcgc gcacggctcg cgcctgggcc tgctcgccac gtttcccgcg agcgtgggct
     1801 cgatggcccg ggagctcgag gcgctcgcgc cgcgcgcgga gctcgagacc gcctgcgtgc
     1861 cgcaggcact ggccgcgctg cgggcgggcg acggcgcgca gcacgacgcc ctgctcgccg
     1921 cggtcgcgcc caagctcagc cattgcgacg cgctgatgct cgcgcagttc tccaccgccc
     1981 gcgcgcgcga agcggtcgcg agagtcgtga agtgcccggt gctcacgagc cccgacagcg
     2041 cggttctcgc gatgcgccgc gcgctcggct gattcgccca tgtgccgcgc gctgctctac
     2101 ctcggcgagc cggtgctgct tgatcatttc ctctaccagc ccgattccgc gctggtgcgc
     2161 caggcctaca tgccaaagat gctcaacatg ctgaacctcg cggggttcgg cctgcgcgcc
     2221 tgggatcccg caagtcacga tcccgcgcgc gcgtacgcct accattcgca ggaggtgccg
     2281 gtcttcgacc gcaacctgaa gaatcttgcg gaaaaggtcc gcgccggctg cctgctcgcg
     2341 cacgtgcgcg gcgtagccga cgagaccgcg gccgacatct cgctgcagaa ctgccacccc
     2401 ttccagttca agggcgtgcc gctcgccctg gcgcataacg gagagctcgc gcggtttgcc
     2461 gagatgaagt ccctcctcgc cgccgccgtg aagcccgagc ttctttcgca gatacgcggc
     2521 accaccgaca gcgagtgggt ctacgccctc ctcgtgtcgc agctgcgcga cccgcatcgc
     2581 cgtccgggcg gcgaggagct cgcccgcgcg gtggaagaca cgctgcgggt cctcggcgag
     2641 gcgcgcgcgc ggctgggcat cgagctgagc tcctcgctca accttttcat cgccgacggc
     2701 acgcagctcg ccgccgtgcg ctactgcttc gactacggct gctacccgac cgacgacgcc
     2761 tcgcacctgc gcgagctgga cttgagctat ctatcgcttt ggtacaccgc cggccgcgac
     2821 tacggactgc acgacggcga atggaagatg accggcgcct ccgagaacgc caactcggtg
     2881 ctgatcgcct ccgagccgct cacacgcgac acggcggcat gggtggaagt cccggagtac
     2941 tccatgctcc acgtcgccct ccgggccggc cggccggcga tcagtatccg ggatctccac
     3001 tgacgatctg gccgccgtag gctttctcga gctggtcgcc ttcgttcctt gcgccgcgca
     3061 gccgcgcctg gctgatgagc cgggtgaggt gccggttcgt ctgccccagc gagtaaagca
     3121 ggcttcgctg gatcgcgcgg tgcagacggc ctgacacggg actgccgccg aagtagagct
     3181 tttcgaaggc atcgcgcggg atttccagaa gaatggcggg ctcgcgcacg atggcgtccg
     3241 ccccgtgcgc gccgcgctcg agtgcgctca tgtagccgag cggctgaccg gggccgagga
     3301 ccgcaatgcg ccggctgcgt tgcgcgtgcc gcgcacggat ttcgaccgcg ccgcgcacga
     3361 cgatgaagca gccggcggag gcctggccgc tgaagaagac agcgtggccg cgcggcaatt
     3421 cgagcagctg ggcaactgcc gcgagcgccg cgatgtcggc cgggtcgaat gcctcgaaga
     3481 ccccaaggtg gggcaggaac ggctcgacgt cgaacgacgc ctgcttcagg cgctcgacgc
     3541 cggcgagcgg atcggcgggg ctgcagggct gcgaggcggc tgcgtcctgc ggtgcgggag
     3601 cttccatcag cttcgcgttc agcgcgcgca gcttgcgcga gatctcgagc gtcaccgcgt
     3661 gctggatgcg tagcgcggcc gccgagcgct gcgcgctcag cgagcggaag tcgtcccggt
     3721 cgatgaacca gccttcgagg ctctctttcg ccgcgacgct cgccgtgcag gtgccgcgtt
     3781 cgatgagcgc ggtctcgccg aacacgctgc ccgcggggag ggtcgcaacg gccagctttt
     3841 cgccgccggg cagcagcacg gtcgcttcgg cggcgccctg ctccaggatg tacgcgccgc
     3901 gcgaaggctc gccatagcgc agcagcc
//
LOCUS       DASUNT010000126         2489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_455735, whole genome shotgun sequence.
ACCESSION   DASUNT010000126 DASUNT010000000
VERSION     DASUNT010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455735"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFX94_02525"
     CDS             complement(<1..450)
                     /locus_tag="VFX94_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767083.1"
                     /translation="MSIDALLAGPVRVINIGLEGFARDLAANGAPVVQVDWKPARFPF
                     DKEVEEANREAVRRILAAEPVLTLVRRAGELIPELDARRLVLHAGPPIEWQRMCAPLR
                     GAVCGALVFEGWASDLATAEELAASGAIDFAPNHHFGAVGPMTGITTR"
     gene            complement(460..1443)
                     /locus_tag="VFX94_02530"
     CDS             complement(460..1443)
                     /locus_tag="VFX94_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEX5767084.1"
                     /translation="MKPREVLVVGGSGFIGRHLVASLASAGLDVSVPSRRRERAKHLI
                     LLPTVDVVQADIMAPGVLERLCSGKQAVYNLVGVLQSRRGRRDHRGPNDYGPDFARFH
                     VELPQAIVAACRNTGVRRLVHMSALGASPTAPSEYLRSKGIGEQAVLAADDLDVTVFR
                     PSVVFGPEDTFLNRFATMARLLPVIGVPCPEARFQPVYVGDVARALHFALDEPETRAK
                     AFELCGPRVYTLKQLVEWVCAVTGRSRLVVGLPDRLSYLQAWMLEHLPGKLMTRDNYR
                     SMQVPSTCDAPFPFRLEPQALEAVAPAWLAPERAPMRPRERYPQLRWRARR"
     gene            complement(1440..>2489)
                     /locus_tag="VFX94_02535"
     CDS             complement(1440..>2489)
                     /locus_tag="VFX94_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5767085.1"
                     /translation="RHLAWKARAALRDGEWKVVRDSIDRMSFGAAHQPAWMYWYGRSL
                     AAQGEEMGSRAYFLRLAGQTDFYGLLAAEELGYVLALPDITHVPTEAQVAAAAADPGL
                     QRALELMRLGLRVEGVREWLFTIRAFDDERLLAAAELARRHGVYDRAIHTADRTSRLH
                     NFSLRYPMPYQDVFRNYAATHGVDEAWVLGLVRQESRFNTEARSGAGAAGLMQVMPRT
                     ARYVAGKMGMRNYRAKSVTEIETNVTLGTGYLKLVMEQLGNPVLASAAYNAGPSRARR
                     WREEKPIEGAVYIESIPFPETRDYVKKVMANAVYYAAVLDRKPVPLKTRLGVIQPPGA
                     WEPPVFEEEPALAAP"
BASE COUNT          400 a          844 c          873 g          372 t
ORIGIN      
        1 gcgcgtggtg atccccgtca tgggacctac cgcgccgaag tggtggttag gcgcgaagtc
       61 gattgcgccc gacgctgcca gctcctcggc ggtcgcgagg tcggaggccc agccctcgaa
      121 cacgagggcg ccgcagaccg ctccgcgcag cggtgcgcac atgcgctgcc attcgatggg
      181 cggtccggcg tgcagcacga ggcgccgggc gtccagctca gggatgagct cgcccgcgcg
      241 gcgcacgagc gtaagcaccg gctccgccgc aaggatccgc cgcaccgctt cccggttggc
      301 ttcctcgact tctttgtcaa acggaaagcg ggcgggtttc cagtccactt ggacaaccgg
      361 cgccccgttc gcggcgaggt cgcgagcaaa gccttcgagg ccgatgttga tcacgcggac
      421 cggccccgcg agcaacgcgt cgatcgacat gtaagagggc tagcggcgcg cgcgccagcg
      481 aagctgcgga tagcgctcgc gcggccgcat gggcgcccgc tcgggtgcga gccaggccgg
      541 tgccacggcc tccagcgcct gtggctcgag ccggaacggg aacggtgcgt cgcaggtgct
      601 cggcacctgc atggagcggt agttgtcgcg cgtcatcagc ttgccgggca agtgctcaag
      661 catccatgcc tgcaagtatg agagccggtc gggcaggccg acgacgagcc gcgagcgccc
      721 ggtcaccgcg caaacccact ccaccagctg cttcaacgtg taaacgcgcg gcccgcacag
      781 ctcgaaagcc ttcgcgcgcg tctccggctc gtcgagcgcg aagtggagcg cgcgcgccac
      841 atcgccgacg tacaccggct gaaaccgcgc ttcgggacaa ggtaccccga tcaccggcag
      901 caaccgcgcc atggtggcaa agcggttaag gaacgtgtct tccggtccga acaccaccga
      961 ggggcggaac acggtgacat cgaggtcatc agcggccagc accgcctgct caccaatgcc
     1021 cttggagcgc aggtactccg agggagctgt gggcgaggcg ccgagcgcgc tcatgtgcac
     1081 gagccggcgc acgccggtgt tgcggcacgc ggcaacgatg gcctgcggca gctcgacgtg
     1141 gaagcgcgcg aaatcagggc cgtagtcgtt tggaccgcgg tgatcgcgcc gcccgcgccg
     1201 gctttgcagc acgccgacga ggttatacac ggcctgcttg ccggagcaca gccgctcgag
     1261 caccccgggc gccatgatgt cggcctgtac cacgtcgact gtgggcagca ggatgagatg
     1321 ctttgcgcgc tcgcgccggc gcgaaggtac gctgacgtcg agtccggcgg aggcgagcga
     1381 agcgacgagg tgccggccga tgaagcccga acccccgacg acgagtacct cgcgcggctt
     1441 catggcgcgg ccagcgcggg ttcttcctcg aacaccggcg gctcccaggc cccgggaggt
     1501 tggatcacgc cgagccgcgt cttgagcgga accggcttcc tgtcgagcac cgcggcgtag
     1561 tacaccgcgt tggccatgac cttcttcacg tagtcgcgcg tctcggggaa cggaatggat
     1621 tctatataca ccgcgccctc gatgggcttt tcttcgcgcc agcgccgtgc gcgcgacggc
     1681 ccggcgttgt acgcggcgga ggcgagaacg ggattcccga gctgctccat cacgagcttg
     1741 agatagccgg tcccaagagt gacattggtt tctatttcgg tcacgctctt ggcgcggtag
     1801 ttgcgcatgc ccatcttccc ggcgacgtag cgggcggtgc gcggcatgac ctgcatcagc
     1861 ccggccgcgc ctgcgcccga gcgcgcttcc gtgttgaagc ggctctcctg gcgtaccagg
     1921 cccagcaccc aggcttcgtc gacgccgtgc gtggcggcat agttgcggaa cacgtcctgg
     1981 tacggcatcg ggtagcgcag gctgaagttg tgcagccgag acgtgcggtc cgcggtatgg
     2041 atggcgcggt cgtagacgcc gtggcggcgc gcaagctcgg ccgcggcgag caggcgctcg
     2101 tcgtcgaacg cacggatggt gaagagccat tcgcgcactc cttcgacgcg tagcccgaga
     2161 cgcatcagct cgagcgcgcg ctgcagaccg ggatcggccg cggccgcggc cacttgcgcc
     2221 tcggtcggca catgggtgat gtccggcagg gcaagcacat agccgagctc ctcggccgcg
     2281 agcaagccgt agaagtcggt ctggccggcg aggcgcagga agtatgcccg gctgcccatc
     2341 tcttcgccct gcgcggcgag cgagcggccg taccagtaca tccatgccgg ctggtgcgcg
     2401 gcgccgaaag acatgcggtc gatcgagtcg cgcacgacct tccactcgcc gtcgcgcagc
     2461 gccgcgcgcg ctttccaggc gaggtgccg
//
LOCUS       DASUNT010000127         2877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1245280, whole genome shotgun sequence.
ACCESSION   DASUNT010000127 DASUNT010000000
VERSION     DASUNT010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245280"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VFX94_02540"
     CDS             complement(<1..238)
                     /locus_tag="VFX94_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase"
                     /protein_id="HEX5767086.1"
                     /translation="MSTSDYPRDLVGYGARPPRPKWPGGARVALQFVLNYEEGGENSV
                     LHGDKASEAFLSEIVGAQPLEGVRHMSMESIYEYG"
     gene            complement(213..1439)
                     /locus_tag="VFX94_02545"
     CDS             complement(213..1439)
                     /locus_tag="VFX94_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HEX5767087.1"
                     /translation="MLDPARIMERCDALARHSELPGGLTRVFLSPEARAATDKVLGWM
                     REAGMQARLDAIGNAAGRYEGERPGLACLMLGSHLDTVRDAGRYDGMLGVVTAIECVS
                     LLNSRKKRPPFAIEVIGFGDEEGVRFGTTLLGSRAVAGTLDMQVLHARDPSGKTIAQA
                     MTDFGLDPARIPQVARKKSDVLAYAELHIEQGPVLEADGLPVGVVTAINGFSRLRATL
                     RGAAGHAGTVPMRLRRDALAGAAECVLAVERIVKGQGDLVGTVGRIEARPGAINVIPG
                     EVLFTIDVRAPQDALRKHAVAAIRAEIEGISRARNLESDVELLQEFGSTACAPQLMGQ
                     MERAVERQGFRVRRLPSGAGHDGMALAAIAGICMLFVRCKGGISHSPLEAITEADAAV
                     GARVLSDFIEHFRLPA"
     gene            1463..1954
                     /gene="uraD"
                     /locus_tag="VFX94_02550"
     CDS             1463..1954
                     /gene="uraD"
                     /locus_tag="VFX94_02550"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEX5767088.1"
                     /translation="MALQEVNALGKAEFVARLGGVFEHSPWIAERAWHKRPFDSIEEL
                     HRAMLAAVRSNDEKLRLCRAHPELAAGDALTSSSTGEQARLGFDALSAPERVDMNRLN
                     RSYREKFGFPCIVALRLHADRQSVMAEIKRRLANDAGTELENALEQIGHITRARLERI
                     LNG"
     gene            1947..2279
                     /gene="uraH"
                     /locus_tag="VFX94_02555"
     CDS             1947..2279
                     /gene="uraH"
                     /locus_tag="VFX94_02555"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEX5767089.1"
                     /translation="MGKLTTHVLDTANGRPGAGIAIDLAMLQAGEWKALKTVKTNADG
                     RTDAPLLEGASFRPGAYELAFHVGSYFKGRGFLDLVPVRFVITDPDAHYHVPLLCSPW
                     GYTTYRGS"
     gene            2282..>2877
                     /locus_tag="VFX94_02560"
     CDS             2282..>2877
                     /locus_tag="VFX94_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5767090.1"
                     /translation="MILRAFLAAVLCLPALLHAQAWPTKPVRVIVTFSPGGSSDIVAR
                     LLAAPLQAELGQSVIVDNKPGAGGTIGALEAARAAPDGYTLLLSNSAPISISPAMQDQ
                     PRYDPVQSFTHVSYIGSVANVFVVHPSVPAKSLRELVGWIKQQPNPVNYGSGGIGSIG
                     HIVGETLKNDQALKMEHVGYKGSAPMHNDLLAGTIKLA"
BASE COUNT          464 a         1010 c          937 g          466 t
ORIGIN      
        1 agccgtactc gtagatcgac tccatcgaca tgtggcgcac gccttcgagc ggctgtgcgc
       61 ccacgatctc ggagaggaac gcctccgacg ccttgtcacc gtgcaggacc gagttttcac
      121 cgccctcctc gtagttcagc acgaactgca gcgcaacgcg cgcgccgccg ggccatttcg
      181 gccggggcgg cctcgccccg tagccgacaa ggtcacgcgg gtagtcggaa gtgctcaatg
      241 aaatcggaca gcacccttgc gcccacggcg gcgtcggcct cggtgatcgc ttcgagcggg
      301 ctgtggctga taccgccctt gcagcgcacg aacagcatgc agatgccggc gatggcggcg
      361 agcgccatgc cgtcgtgacc cgcgcccgac ggcaggcggc gcacccggaa gccctggcgc
      421 tcgaccgcgc gctccatctg tcccatcagc tgcggcgcgc acgccgtgga cccgaattcc
      481 tgcagcagct cgacgtcgct ttcgaggttt ctcgctctcg aaatgccttc gatctcggcg
      541 cgaatcgctg ccaccgcgtg cttcctcagc gcgtcctggg gcgcgcgtac gtcgatggtg
      601 aagagcactt cgccgggaat gacgttgatc gcgccgggcc gcgcctcgat ccgtcccacc
      661 gtgccaacga ggtcgccttg ccccttgacg attctctcta ccgcaagaac gcattccgca
      721 gcgcccgcga gcgcgtcgcg ccgaagacgc atcggcaccg tgccggcatg cccggcagcg
      781 ccgcgcagcg tggcgcgcag ccgcgagaag ccgttgatgg ccgtgacgac gcccaccggc
      841 aggccgtcgg cctcgagcac cgggccctgc tcgatgtgga gctcggcgta ggcgagcaca
      901 tcgctcttct tgcgagcgac ctgcggtatg cgcgccggat cgagtccgaa gtcggtcatc
      961 gcctgcgcga tcgtcttgcc gctcggatct cttgcatgca agacctgcat gtcaagtgtc
     1021 cccgcgaccg cccggctgcc gaggagcgtg gtgccgaagc gaacgccttc ctcgtcaccg
     1081 aacccgatca cttcgatcgc gaagggcggc cgtttctttc tcgaattcag caaactcacg
     1141 cattcgatcg ccgtcaccac gcccagcatg ccgtcgtagc ggccggcgtc gcggaccgtg
     1201 tcgaggtgcg agccgagcat caggcaggca agacccgggc gctcgccctc gtagcgcccg
     1261 gccgcgttgc caattgcgtc gagccgtgcc tgcatgccgg cttcgcgcat ccagccgagc
     1321 accttgtcgg tggccgcgcg cgcttcggga gagaggaaca cccgggtgag gccgccggga
     1381 agctcggaat gccgcgcgag cgcgtcgcag cgctccatga tgcgcgcggg gtcgagcatg
     1441 cgccgagtgt acactagggt gcgtggcgct ccaggaggtg aacgcgctcg ggaaagcgga
     1501 gttcgtcgcg cggctcggcg gcgtgttcga gcactcgccg tggattgccg agcgcgcgtg
     1561 gcacaaacgc cctttcgact cgatcgagga attgcaccgc gcgatgctcg ccgccgttcg
     1621 gtccaacgat gaaaagctgc ggctctgccg cgcgcacccc gagctcgccg caggagatgc
     1681 gctcacgtcg tcttcgaccg gcgagcaggc ccggcttgga ttcgatgcgc tttcggcgcc
     1741 ggagcgcgtg gacatgaacc ggctgaaccg cagctaccgc gaaaagttcg gtttcccgtg
     1801 catcgtggcg ctgcggctgc atgccgaccg gcaaagcgtg atggccgaga taaagcgccg
     1861 cctcgcgaac gacgcgggca ccgagctcga gaacgcgctc gagcagatcg gccacatcac
     1921 gcgcgccagg ctggagagaa tcctcaatgg gtaagctcac gacgcacgtg ctggacacgg
     1981 cgaacggaag accgggtgcg gggatcgcca tcgatcttgc gatgctgcag gctggcgaat
     2041 ggaaggcgct caagaccgtc aagaccaacg ccgacgggcg caccgatgct ccgcttctcg
     2101 aaggcgcgag cttccggccc ggcgcttacg agcttgcttt ccatgtcggc agctacttca
     2161 aggggcgcgg gttccttgat ctcgtgccgg tgcgcttcgt gatcaccgac ccggacgcgc
     2221 attaccatgt gccgctgctt tgctcgccgt ggggctacac cacttacagg gggagctgac
     2281 aatgatcttg cgcgcattcc tcgccgccgt gctatgcctg ccggccctgc tgcatgccca
     2341 ggcgtggccc accaagccgg tgcgggtcat cgtgaccttc tcgccgggcg gttcttccga
     2401 catcgtcgcg cgcctgctcg ccgcgccgct gcaggccgag ctcggccagt cggtgatcgt
     2461 cgacaacaag cccggcgccg gcggcacgat cggcgcgctc gaggccgcgc gcgccgcgcc
     2521 cgacggctac acgctgctcc tctcgaacag cgccccgatc agcatctcgc ctgcgatgca
     2581 ggaccagccg cgctacgacc cggtgcagag ctttacccac gtctcgtaca tcggctcggt
     2641 ggccaacgta ttcgtggtgc atccctcggt gcccgcgaag tcgctgcgcg agctcgtcgg
     2701 ctggatcaag cagcagccca acccggtgaa ctacggctcc ggcggcatcg gctcgatcgg
     2761 ccacatcgtc ggcgaaacgc tcaagaacga ccaggcgctc aagatggagc acgtcggtta
     2821 caagggctcg gcgccgatgc acaacgacct ccttgcgggc acgatcaagc tcgccat
//
LOCUS       DASUNT010000128         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_981442, whole genome shotgun sequence.
ACCESSION   DASUNT010000128 DASUNT010000000
VERSION     DASUNT010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981442"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VFX94_02565"
     CDS             <1..424
                     /locus_tag="VFX94_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5767091.1"
                     /translation="SRIRSLVLVSPAGVTAPGLRAADIFLMAPEEMVRNLFVDPKLAE
                     ARLAQPEDIDVSLKNRHTTARLAWEPRYHDPFLPKWLHRIDVPVKIVWGREDRIIPAG
                     LAAVIARRIPGAQVHILENCGHLPHAEKADELVDIVCR"
     gene            415..1554
                     /locus_tag="VFX94_02570"
     CDS             415..1554
                     /locus_tag="VFX94_02570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5767092.1"
                     /translation="MQVTLFHLMPYAEVDVAAARAHGTVWGLPNKHYDAQVAHRLYNR
                     FLDELEYGEELGFDALSVNEHHQTAYGLMPSPIVTASALARRTRRAKIAILGSALPLR
                     SHPMMVAEEHAMIDVISGGRLISGFVRGIGAEYHTFGVNPTFSHERFHEAHDLIVRAW
                     TQKGPFAFEGRHYRVSYVNLWPQPLQKPHPPIWIPSQGSKETIDWAAHPERRYTYLQT
                     FSPAKVVARYLRMYRDTCEGYGYQAADAQLGWSVPVYVATSDESARREARIHFENFRN
                     VFVRMPLEMLLPPGYTSRDSLKNVMKAKAQMFGDVTLEQALELGLFVCGSPASVLSTL
                     RNHWQEMRFGNLLVLCQFGTLPADLTRRNMELFAREVMPALKSLS"
     gene            complement(1547..1801)
                     /locus_tag="VFX94_02575"
     CDS             complement(1547..1801)
                     /locus_tag="VFX94_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767093.1"
                     /translation="MALPIIKLGLAAAAGYYFYNRFRKSRVAGSTTNASDAVDDVKLG
                     PNPSADEKLDAGVQETFPASDPVSVAQPSETAYERKQRSS"
     gene            1837..>2295
                     /locus_tag="VFX94_02580"
     CDS             1837..>2295
                     /locus_tag="VFX94_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX5767094.1"
                     /translation="MPVNHAGERKYEMNVGFIGLGQMGRPMVERLKGAGHHVKTYNRT
                     RTSAEVHDKPEAVLDSEVVITMLSDDAAVRAVWLDTELAKRLPRTAVHLNMATVSMRI
                     ARELTAVHGDSYVAAPVFGRPPLAAQGQLDIIAAGPHGALQKCDPLFRVLG"
BASE COUNT          394 a          760 c          753 g          388 t
ORIGIN      
        1 ctcgcgcatc cggtcgctcg tcctcgtgag ccccgccggc gttacggctc ccgggctgcg
       61 cgcggccgac atcttcctca tggcgccgga ggagatggtc aggaatctct tcgttgaccc
      121 gaagctggcc gaagcgcgcc tggcgcagcc cgaggacatc gacgtctcgc tcaagaaccg
      181 gcacaccacc gcgcgcctcg cctgggagcc gcgctaccac gatcctttcc tgccgaagtg
      241 gctgcaccgc atcgacgtgc cggtgaagat cgtctggggc cgcgaggatc gcatcatccc
      301 ggcgggcctc gccgcggtga ttgccaggcg cattccgggc gcgcaggtgc acatcctgga
      361 gaactgcggc caccttccgc atgccgagaa agcggacgag ctcgtggaca tcgtatgcag
      421 gtaacgctgt tccacctcat gccgtacgcg gaggtggatg tcgccgcggc acgcgcgcac
      481 ggcacggtat gggggttgcc gaacaagcac tacgacgcgc aggtcgccca tcggctgtac
      541 aaccgctttc tcgacgagct cgagtacggc gaggagctcg gcttcgacgc gctctcggtg
      601 aacgagcatc accagacggc ctacggcctg atgccttcgc cgatcgtgac cgcgtcggcg
      661 ctcgcgagaa ggacccggcg ggcaaagatc gcgatccttg gcagcgcgct gccgcttcgc
      721 tcgcatccga tgatggtggc cgaagagcac gcgatgatcg acgtgatcag cggcgggcgc
      781 ctgatcagcg gcttcgtgcg cggcatcggc gccgagtacc acaccttcgg cgtcaacccg
      841 acgttctcgc acgagcggtt ccacgaggcg cacgacctca tcgtgcgcgc ctggacacag
      901 aagggtccgt ttgccttcga gggccgacat taccgcgtca gttacgtcaa cctctggccg
      961 cagccgctgc agaagccgca tccgccgatc tggatcccct cgcagggcag caaggagacc
     1021 atcgactggg cggcgcaccc cgagcggcgc tatacgtacc tgcagacctt cagcccggcc
     1081 aaggtcgtgg cgcgctatct caggatgtac cgagacacct gcgaaggcta cggctaccag
     1141 gcggccgacg cccagctcgg ctggtccgtc cccgtctatg tggcgacgag cgacgaaagc
     1201 gcccgcaggg aagcacgaat ccatttcgag aacttccgca atgtgttcgt gcgcatgccg
     1261 ctcgagatgc tgctgccgcc gggctacacg tcgcgcgatt cgctgaagaa cgtgatgaaa
     1321 gccaaagccc agatgttcgg cgacgtgacg ctcgagcagg cgctcgagct cggtctcttc
     1381 gtgtgcggca gcccggctag cgtcctgagc accttacgta atcactggca ggaaatgcgc
     1441 ttcggcaacc tgctcgtgct gtgccagttc ggcacgctgc cggccgacct cacgcgccgg
     1501 aacatggagc tcttcgcgcg cgaggtgatg ccggcgttga aatcgctcag ctagaacgct
     1561 gctttcgctc gtaagcggtc tcggaaggct gggcgacgct caccgggtcg cttgccggaa
     1621 acgtctcctg aacgccggcg tcgagcttct cgtcggcgct ggggttggga cccagcttga
     1681 cgtcgtcgac ggcgtcgctc gcgttcgtgg tggagccggc gacgcgcgat ttccggaaac
     1741 ggttgtaaaa gtaatagccc gccgccgctg cgaggccgag cttgatgatg ggaagggcca
     1801 tggtatgtct cctttcgccc ttcgcttgca accgtcgtgc ccgtcaacca tgccggggaa
     1861 aggaaatatg aaatgaatgt cgggtttatc ggtctcggcc agatgggtcg acccatggtg
     1921 gagcggctca agggcgcggg gcatcacgtg aaaacctaca accgcacgcg aacctccgca
     1981 gaggtgcacg acaagcccga ggcagtgctc gacagtgagg tcgtgatcac gatgctgtcc
     2041 gacgacgccg cggtgcgcgc ggtatggctc gacaccgagc tcgcgaagcg gctgccgcgg
     2101 accgcggtgc acctcaacat ggcgacggtg agcatgcgca tcgcgcgcga gctgaccgcg
     2161 gtccacggag attcgtacgt cgccgcgccg gtgttcggcc gcccgccgct tgcggcccag
     2221 ggccagctcg atatcatcgc tgccgggccg cacggtgctc tgcagaaatg cgatccgctg
     2281 ttccgcgtcc ttggc
//
LOCUS       DASUNT010000129         2437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_718769, whole genome shotgun sequence.
ACCESSION   DASUNT010000129 DASUNT010000000
VERSION     DASUNT010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718769"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VFX94_02585"
     CDS             <1..832
                     /locus_tag="VFX94_02585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5767095.1"
                     /translation="EGRFILGVSPGALVSDAEALGIMDEDRNKMFAEAIDVILEIWSR
                     EPPYEIDLPGNRFKVTTKKTMHLETGVGIMPKPYQKPRPEIVGTVVAPFSKGVVLMGE
                     RDFHPLSANFLLPHWLKTHWANYAEGKTKAGIKPDPAEWRVARTVFVADDDKVAAAYG
                     RYDANSPYRFYYKQLQTKLTKAGRHAVFKERLDQPDHEITYEFVLDRLCIYGTVNKVV
                     DQILQLRQMVGDFGELVYAGMDWVDPKLAKRSMQLMAEEVMPRVNAAIAKENPRAAAA
                     "
     gene            876..1160
                     /locus_tag="VFX94_02590"
     CDS             876..1160
                     /locus_tag="VFX94_02590"
                     /inference="COORDINATES: protein motif:HMM:NF040427.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6455 family protein"
                     /protein_id="HEX5767096.1"
                     /translation="MIRRLLRAWRQARREIEKLELWQVLHRRGTRRLASHNPQALAFA
                     VERCTGCRQAPQCGQEIAAGSDRAIETFCPNTMYLRHLDAMRRHAVTAKR"
     gene            complement(1144..1851)
                     /locus_tag="VFX94_02595"
     CDS             complement(1144..1851)
                     /locus_tag="VFX94_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5767097.1"
                     /translation="MAFVPGGYGGLGAAICGGLAAQGAKVIVAGRDKRRATALARKIR
                     GIAVEMDVRTPSSIENSLAPFPEIDILVNCAGVQREEAFDEVTEDAWDEVLGTNLKAA
                     MFLAQATAKRQRRGGKQVHLLSVRAQLGLRGRGYSAYCAAKGGLAMLVRQHACELAPR
                     GICVNGVAPTVVRTRMGAHWLKNPKTRAWLKERIPLGRVAETRDVVGAVLFFCSPASD
                     YVTGQILYVDGGITASQ"
     gene            complement(1866..>2437)
                     /locus_tag="VFX94_02600"
     CDS             complement(1866..>2437)
                     /locus_tag="VFX94_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5767098.1"
                     /translation="SDLQCDISDAAAVSSAMKRIGQVDILVNNAGILSNNKAEATTPE
                     EWHKVLAANLDGAFYLAREVIPGMKARGWGRIVNTCSLAAKTGGLTAGTAYAVSKGAL
                     TSLTFSLARELAPFGVTVNGISPAYVKTPMVTEQLTEAQRQALIAQIPVGRFCEPEEF
                     AHVVRFLVSPLAGFITGEIVDLNGGLVMD"
BASE COUNT          427 a          839 c          793 g          378 t
ORIGIN      
        1 cgagggccgc ttcatcctcg gcgtgagtcc cggggcgctc gtgtccgacg ccgaggcgct
       61 cggcatcatg gacgaagacc gcaacaagat gttcgccgag gcgatcgacg tgatcctcga
      121 gatctggtca cgcgagccgc cctacgagat cgacctgccg gggaaccgct tcaaggtgac
      181 gacgaagaag acgatgcacc tcgagaccgg cgtgggcatc atgcccaagc cgtaccagaa
      241 gccgcgcccc gagatcgtcg gcaccgtggt cgcgcccttc tccaaggggg tggtactcat
      301 gggcgagcgc gacttccacc cgctgtcggc gaacttcctg ctgccgcact ggcttaagac
      361 gcactgggcg aactacgccg agggcaagac caaggccggg atcaagcccg accccgccga
      421 gtggcgcgtc gcgcgcaccg tgtttgtcgc cgacgacgac aaggtcgccg ccgcctacgg
      481 ccgttacgac gcgaacagcc cgtaccgctt ctattacaag cagctgcaga ccaagctcac
      541 caaggcgggc cgccacgcgg tgttcaagga gcgcctcgac cagcccgacc acgagatcac
      601 ctacgagttc gtcctcgatc gcctctgcat ctacggcacg gtgaacaagg tagtcgacca
      661 gatcctgcag ttgcgccaaa tggtgggcga cttcggcgag ctcgtctacg cggggatgga
      721 ctgggtcgat ccgaagctcg ccaagcgctc catgcagctc atggcggagg aagtaatgcc
      781 gcgcgtcaac gcggcaattg caaaagaaaa tccgcgcgcc gcggcggctt gatcccggtc
      841 aaccgggacc gggagcggag ccctaagctg cgcgcatgat tcgacgcctg ctcagggcct
      901 ggcggcaagc caggcgggaa atcgaaaagc tcgagctctg gcaagtgctg caccggcgcg
      961 gcacccgccg gctcgccagc cacaacccgc aggcgctcgc cttcgcggtc gagcgctgca
     1021 ctggctgccg gcaggcgcct cagtgcggcc aggagatcgc ggccggatcc gatcgcgcta
     1081 tcgaaacctt ctgtccgaac accatgtacc tacggcacct cgacgcgatg aggcgccacg
     1141 ccgtcactgc gaagcggtga tgccgccgtc cacgtagagg atctgcccgg tgacgtagtc
     1201 gctcgccggg gagcagaaga agagcacggc gccgaccacg tcgcgcgttt ccgcgacgcg
     1261 cccgagcggg atgcgctcct tcagccaggc gcgcgtcttg ggattcttga gccaatgcgc
     1321 gcccattcgc gtgcgcacca ccgtcggcgc caccccgttc acgcagatgc cgcgcggcgc
     1381 gagctcgcag gcgtgctggc gcacgagcat ggcgagcccg cccttggcgg cgcaatatgc
     1441 cgagtagccg cgcccgcgca gcccgagctg cgcgcgtacc gagagcaggt gcacctgctt
     1501 gccgccgcgc cgctggcgct tggccgtcgc ctgcgcgagg aacatcgccg ccttgaggtt
     1561 ggtgccgagt acctcgtccc aggcgtcctc cgtaacctcg tcgaacgcct cttcgcgctg
     1621 caccccggcg cagttcacga ggatgtcgat ttccgggaag ggggcaagcg agttctcgat
     1681 ggacgaaggc gtgcgcacat ccatttcaac cgcaatgccg cgtatcttgc gggcgagggc
     1741 tgtcgccctg cgtttatcgc gtccggcgac gatgactttc gctccctgcg cggccagccc
     1801 gccgcatatc gcggcgccga gaccgccgta gccgccgggc acgaacgcca ctttgcccct
     1861 gagactcaat ccatcaccag gccgccgttg agatcgacga tctcgccggt gatgaagccg
     1921 gcgagcggcg agacaagaaa acgcaccacg tgcgcgaact cctcgggctc gcagaagcga
     1981 cccacgggta tctgtgcgat gagcgcctgg cgctgcgcct cggtgagctg ctcggtgacc
     2041 atcggcgtct tcacgtacgc gggcgagatc ccgttcaccg tcacgccgaa cggcgcgagc
     2101 tcgcgcgcaa gcgagaacgt aagcgaggtg agggcgccct tggacaccgc gtacgccgtg
     2161 ccggcggtca ggccgccggt cttcgccgcg agcgagcatg tgttgacgat gcgtccccag
     2221 ccccgcgctt tcatgccggg gatcacctca cgcgcgagat agaacgcacc gtcgaggttg
     2281 gccgcgagca ccttgtgcca ttcttccggc gtcgtggctt ccgccttgtt gttggacagg
     2341 atgccggcgt tgttgaccag tatgtcgacc tgcccgatgc gtttcattgc ggaagaaacg
     2401 gcagccgcgt ccgagatgtc gcactgcagg tccgaac
//
LOCUS       DASUNT010000130         3471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_631420, whole genome shotgun sequence.
ACCESSION   DASUNT010000130 DASUNT010000000
VERSION     DASUNT010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631420"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFX94_02605"
     CDS             complement(<1..273)
                     /locus_tag="VFX94_02605"
                     /inference="COORDINATES: protein motif:HMM:NF023665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HEX5767099.1"
                     /translation="MDRNDSLSTRRIHTDADCYDMMYWSIKFGVSMNRLLEAVDKVGP
                     LVPDVERHLNGSTAMMAEPIADNRARPVLHFDRLSRAGLADRRIVRP"
     gene            complement(429..1973)
                     /locus_tag="VFX94_02610"
     CDS             complement(429..1973)
                     /locus_tag="VFX94_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX5767100.1"
                     /translation="MTWALPASASIRTERHFGERLVRCFADRPANVPEAFASACEARP
                     EAQALVCGEERLTYAELAHRSARVAAGLAARGIKAQDRIGLMLGNRVEFATALLGILR
                     LGAIAVPINTRLQRPEVAYILAQCRARVLVHESNLASKVPALEHLLDERQLEGDGEAP
                     ALASMKEEDTAIILYTSGTTGRPKGAMLTHFNIVHSLLHYKYCFRLGEGDRALLAVPA
                     SHVTGVVAILLAIWHAQGCAVVMPEFKAPNFLELAARERITYTILVPAMYNLCLLQPD
                     FDRFELSNWRVGGYGGAPMPEATIATLARKLPQLGLHNAYGSTETTSPSTLMPAGCTA
                     ERPDSVGLPVPCAEIIVVDADANELPPGETGELWIRGPMVVPGYWDNPEATRQSFTNG
                     YWRSGDLGSIDRDGFVRVFDRLKDMINRGGYKVYSVEVENVLSHHPAVLESAIVGKPC
                     PVLGERVHAFVTLKEPASTEELKRYCAERLADYKVPETFTLLERPLPRNANGKLLKRE
                     LRDAVS"
     gene            complement(1970..2596)
                     /locus_tag="VFX94_02615"
     CDS             complement(1970..2596)
                     /locus_tag="VFX94_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5767101.1"
                     /translation="MSIGPVSILRGVSLGVGTGEFAGLIGRNGAGKTTLLRTVMGLLP
                     PAGGWLGYDDRPLAGLPTHRRTSLGIGYMPEDRRLVPGLSVEENVLLPAWAARFADVT
                     GRLQRVYDAIPEVFELRARKALHLSGGQQKLVALARAMMAGSRLLLLDEPFEGVAPVL
                     ARRLAQVIAGLRQTGVSAILSESSLAHAHGLLDRVFTIDRGAVASSSQ"
     gene            complement(2607..3335)
                     /locus_tag="VFX94_02620"
     CDS             complement(2607..3335)
                     /locus_tag="VFX94_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5767102.1"
                     /translation="MSVLLSAERLDKRFGAVTAASDITLEFARDSVVSLIGANGAGKT
                     TFLNMVTGYLRPDSGRIRFDGHDLVGRSPREITGMGLSRSFQIPQLFAALSVRENLLV
                     AEGIAHAEHAEQATEEVLARFRLEPYAGRAAGLLPEGIRKLLDVAMALVARPKLLLLD
                     EPTSGVAAEEKFGIMDLVMTAVRGQGVTVLFVEHDMDIVSRYAGRVVAFYDGRIIADG
                     MPTAVLRSEEVRRYVIGEDVRAAA"
BASE COUNT          583 a         1155 c         1178 g          555 t
ORIGIN      
        1 gggccggaca atcctgcggt ctgcaagtcc ggcgcggctc aggcggtcga aatgcagcac
       61 aggacgcgcc cggttgtccg ctattggttc agccatcatt gccgtactcc cgttcaggtg
      121 ccgctcgaca tccggcacca gcggacccac cttgtcgacc gcctcgagca agcgattcat
      181 ggagacgccg aactttatcg accagtacat catgtcgtag cagtcagcgt cggtgtggat
      241 gcgccgcgtg ctcaatgagt cgttccgatc catttccata tacctccttc ggagagaacg
      301 caggtagcca catgccgttg acgacgaccg tccagcgttc gcgtcaagcg gacccgtagc
      361 ctccagaagc gttgcttcgt cacgacgcta acgtagcgcc gcgcaccgga actgactgtc
      421 aaactacgtc aggagacagc gtcgcgtaac tcgcgcttca gcagcttgcc gttagcgttg
      481 cggggcaggg ggcgctcgag cagggtgaag gtttcgggca ctttgtagtc ggccagccgc
      541 tccgcgcagt agcgcttgag ctcctctgtc gaagccggct ccttgagcgt cacgaaggca
      601 tgcacgcgct cgccgagcac ggggcagggc ttgcccacga tcgcgctctc gagaaccgcg
      661 ggatggtggc tgagaacgtt ctccacttcc acgctgtaaa ccttgtaacc gccgcggttg
      721 atcatgtctt tcaggcggtc gaagacgcgc acgaagccgt cgcggtcgat ggagccgagg
      781 tcgcccgagc gccagtagcc gttggtgaac gactggcgcg tcgcttcggg gttgtcccaa
      841 tagccgggaa ccaccatggg cccgcggatc cagagctcgc ccgtctcgcc gggcgggagc
      901 tcgtttgcgt cggcgtccac cacgatgatt tccgcgcatg gcacaggaag cccgacgctg
      961 tcgggacgct cggcggtgca cccggccggc atgagcgtcg agggcgaggt ggtttccgtg
     1021 gagccgtagg cattgtgcag cccaagctgg gggagcttcc gcgcgagcgt ggcgatggtc
     1081 gcctcgggca tgggcgcgcc gccatagccg ccgacgcgcc agttggagag ctcgaagcga
     1141 tcgaagtccg gctgcaggag gcataagttg tacatcgcgg gcacgaggat ggtgtaggtg
     1201 atccgctcgc gcgccgccaa ctcgaggaag ttgggggcct tgaactccgg catcacgacg
     1261 gcgcagccct gcgcatgcca gatcgcgagc aggatcgcca ccacgccggt gacatggctc
     1321 gcgggcaccg caagtagggc tctgtcgccc tccccgaggc gaaagcaata cttgtagtgc
     1381 agcagcgagt gcacgatgtt gaagtgcgtc agcatcgcgc ctttgggccg gcccgtggtg
     1441 ccggaggtgt aaagaatgat ggcggtgtct tcctccttca tggacgcgag cgctggcgcc
     1501 tcgccgtcgc cttcgagctg gcgctcatcg aggaggtgct cgagcgccgg gaccttggac
     1561 gcgaggttcg attcgtgcac gaggacccgt gcacggcact gtgcgaggat gtaggccacc
     1621 tccggtcttt gcaggcgcgt gttgatcggt accgcgatgg cgcccagccg caggatgccg
     1681 agcagcgcgg tggcgaactc cacccggttg cccagcatca acccgatccg gtcttgcgct
     1741 ttgataccgc gtgcagcgag gccggcagct actcgagcgg aacggtgcgc aagctcggcg
     1801 taggtcaggc gctcttcccc gcaaaccagc gcctgcgcct cgggccgcgc ctcgcaggcg
     1861 ctggcgaatg cttccgggac attggcgggg cgatccgcga agcagcgcac caggcgctcg
     1921 ccgaaatgcc gctccgtccg gatgctcgcc gaggcgggaa gtgcccaggt cattgcgacg
     1981 acgacgcaac ggcgccgcgg tcgatcgtga acacgcggtc gagcaggccg tgggcgtgcg
     2041 cgaggctcga ttcggacagg atcgcgctca cgccggtctg gcgaaggccg gcgatgacct
     2101 gggcgagccg ccgcgcgagc accggcgcga cgccctcgaa cggctcgtcc agaaggagaa
     2161 gccggctgcc cgccatcatg gcgcgcgcca gcgcaacgag cttctgctgg ccgcccgaga
     2221 ggtgcagcgc cttgcgtgca cgcagctcga acacctccgg aatggcgtca tagacgcgtt
     2281 gcagacggcc cgtgacgtct gcgaaccgcg cagcccacgc cggcaggagc acgttctctt
     2341 cgacgctcag tcccggcacg agccgccggt cctccggcat gtagcctatt ccgagcgagg
     2401 ttctgcggtg cgtcggcagc ccggcgagcg gcctgtcgtc gtaaccgagc caacccccgg
     2461 ccgggggcag cagccccatc acagtgcgga gcagcgtcgt cttgccggcc ccgttgcggc
     2521 cgatgaggcc ggcgaattcg ccggtcccga cgccgaggct gacgccgcgc aggatggaca
     2581 cgggcccgat ggacacggag acgttctcaa gccgcagcac gcacgtcctc gccgatcacg
     2641 tagcggcgta cctcttcgga tcgcagcact gcggtcggca tgccgtccgc gatgatgcgc
     2701 ccatcgtaga aagcgaccac gcgtccggcg tagcgcgaga cgatatccat gtcgtgctcc
     2761 acgaaaagca cggtgacccc ctggccgcgc actgccgtca tcacgaggtc catgatgccg
     2821 aacttctcct cggcggccac gccgctcgtg ggctcgtcga gcagcaggag cttgggccgc
     2881 gcgacgagcg ccatcgccac gtcgagcagc ttgcggatgc cctccggcag gagtccggcg
     2941 gcccgcccgg catacggctc caggcgaaag cgcgcaagga cttcctcggt cgcctgctcg
     3001 gcgtgctcgg cgtgcgcgat gccctcggcg acgagcaggt tctcgcgcac gctgagcgcg
     3061 gcgaagagct gggggatctg gaacgagcgc gacagcccca tgccggtaat ctcgcgcggc
     3121 gagcggccga caaggtcatg accatcgaag cggatacgcc cgctgtcggg ccgcagatag
     3181 ccggtgacca tgttgaggaa ggtcgtcttg ccggcgccgt tcgccccgat caggctgacg
     3241 acgctgtcgc gcgcgaattc gagcgtgatg tccgacgccg ccgtcacggc gccgaaacgc
     3301 ttgtcgagtc gctcggccga aagcagcacg ctcatgccgg ctttctcttc agcagcgtcc
     3361 acaatccacc gggcaggaac atgatgatcg cgagcatcgc cgcgccgagc gccatctgcc
     3421 agatgttggg cgcgtactgg ctcgccacgg tgcgcaacgc ctcgaagatc g
//
LOCUS       DASUNT010000131         2865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_280728, whole genome shotgun sequence.
ACCESSION   DASUNT010000131 DASUNT010000000
VERSION     DASUNT010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280728"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /gene="mutS"
                     /locus_tag="VFX94_02625"
     CDS             <1..1393
                     /gene="mutS"
                     /locus_tag="VFX94_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX5767103.1"
                     /translation="LLKELIGDLATPEEALFILEHAIHPEPSARVVDGGVIADGYNAD
                     LDELRELQSNAGGFLVDFERREKERTGIPNLRVAYNHVHGYYIEVTNSHAEKVPDDYR
                     RRQTLKNAERYITPELKRFEDKALSARDRALALERSLYDEVVEQLQAHLPSFQQIARA
                     LAQADVLSTFAALAVRRNYARPQFTHELCIDIDAGRHAVVEGQIESFIANDTRLSPTR
                     RLLLITGPNMGGKSTYMRQVALIVLLAHVGSFVPARAARIGPIDQIFTRIGAADDLAG
                     GRSTFMVEMTESAAILHNATEKSLVLMDEVGRGTSTFDGLALAWAIANHLIRTNRCLS
                     LFATHYFEMTRLALEYKEVANVHLDAVEHKDTVVFLHAVEEGPASRSYGLQVAGLAGV
                     PRPVIRQAKKYLRLLEDASLARGNQEDLFSTKIHEEPEPAVDPLREELQKVNPDELSP
                     REALELLYRLKRL"
     gene            1390..2496
                     /locus_tag="VFX94_02630"
     CDS             1390..2496
                     /locus_tag="VFX94_02630"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5767104.1"
                     /translation="MKPLEGLRILTVENFGAGPYGTMFLAGLGAEVTKIENAATGGDA
                     SRHVGPRLLGEDDSEYFQTFNLWKKTVSLDLTKDRSKFLRLVKGSDGVVNNLRGDVPA
                     KLGLDYRSLSSVNEKIVCLHISAYGRDNERAFWPGYDFLMQAEAGLMDLTGEPEGAPS
                     RFGTSIIDCMSGVTGALALLACVLRAKRTGKGCDVDASLFDVALHQLTYMGTWHLNGG
                     KPSSRQPRSAHYSLTPVQTFPTADGWIFVMCMKESFWSALCEALSRPDLGRDARFATM
                     AARLENRATLTGMLDDEFRRKTTAHWLKALSGRLPVGPVNDLRAALANPFVAATGMVR
                     SIEHPEKRGLRVLTLPVKIDGERPQPVVCEGLKK"
     gene            2493..>2865
                     /locus_tag="VFX94_02635"
     CDS             2493..>2865
                     /locus_tag="VFX94_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5767105.1"
                     /translation="MKLQGIRVVDLSLFLPGPYLTLILADHGAEVMKVEPPGEGDPTR
                     HLGPKDGGETVYFRNLNRGKKSLVVDLKNPQEREALLELCDTADVFVESFRPGVADRL
                     GVGYQALRARNPRLVYCSISAF"
BASE COUNT          527 a          950 c          919 g          469 t
ORIGIN      
        1 gctcctcaag gagctgatcg gcgatctcgc gacgccggaa gaagcgctct tcatcctcga
       61 gcacgccatt caccctgagc cctccgcccg cgtcgtcgac ggcggcgtga tcgccgacgg
      121 ctacaacgcc gacttggacg agctgcgaga gcttcagtcg aatgccggcg gttttctggt
      181 ggattttgaa aggcgggaaa aagaaagaac cgggattccg aatctgcgcg tggcctacaa
      241 ccacgtgcac ggctactaca tcgaggtgac caactcccat gccgaaaaag tccccgacga
      301 ctaccgccgc cgccagacac tgaagaacgc cgagcgctac atcacgccgg agctcaagcg
      361 gttcgaggac aaggcgctgt ccgcgcgcga ccgcgccctg gcgctggaga ggtccttata
      421 cgacgaggtc gtcgagcagc tgcaggccca tttgccttcc ttccagcaga tcgcgagagc
      481 gctcgcgcag gcggatgtcc tttccacgtt cgcagccctc gcggtcaggc ggaactacgc
      541 gcggccgcag tttacacacg agctgtgcat cgacatcgac gcggggcgcc acgccgtggt
      601 cgagggccag atcgagagct tcatcgcgaa cgacacacgg ctctcgccaa cgcggcgctt
      661 gctgctcatc accggcccga acatgggcgg caagtcgacc tacatgcgcc aggtggcgct
      721 tattgtgctt ctcgcgcacg tcggctcgtt cgttcccgcg cgggcggcgc gcatcggccc
      781 gatcgaccag atcttcacgc gcatcggcgc agccgacgac ctcgccggcg ggcgctcgac
      841 gttcatggtt gagatgaccg aaagcgcggc gatcctgcac aacgccacgg agaagagcct
      901 ggtactgatg gacgaagtcg gccgcggcac ctcgaccttc gacggcctcg cccttgcctg
      961 ggcaatcgcg aatcacttga tcagaaccaa caggtgtctc agcttgttcg ccacgcacta
     1021 cttcgagatg acgcgccttg cgctcgagta caaggaggtg gcgaacgtcc accttgacgc
     1081 tgtcgagcac aaggacaccg tcgtgttcct gcacgcggtt gaggaaggcc ccgcgagccg
     1141 cagctacggc ctgcaggtcg ccgggctcgc cggcgtgccg cgcccggtga tccggcaggc
     1201 caagaagtat ctccggctcc tggaagatgc gagcctcgct cgcggcaacc aggaggacct
     1261 gttctcgaca aagatccatg aagaacccga gcccgcggtc gacccgctgc gcgaggagct
     1321 gcagaaggtg aatcccgacg agctctcgcc gcgcgaagcg ctcgagctcc tctataggct
     1381 caagcgcctg tgaagccgct cgaaggtctg cgcatcctta cggtcgagaa tttcggcgct
     1441 ggcccgtacg gcacgatgtt cctcgccggc ctgggcgcgg aggtgaccaa gatcgagaac
     1501 gccgcgaccg gcggcgacgc gagccggcac gtcgggccgc gtcttctagg cgaggacgac
     1561 agcgagtact ttcagacctt caacctttgg aagaagacgg tttcgctcga cctgacaaag
     1621 gatcgaagca aattcctgag actggtgaag ggctcggacg gggtcgtgaa caacctacga
     1681 ggcgacgtgc cggcgaagct ggggctggac tacaggagcc tttcatcggt caacgagaag
     1741 atcgtctgcc tgcatatctc cgcctacggc cgcgacaacg agcgcgcctt ctggccgggc
     1801 tacgacttcc tgatgcaggc cgaggcgggg ctcatggacc tcaccggcga gcccgaaggc
     1861 gcgccgagcc gcttcggcac ttcgatcatc gattgcatga gcggcgtgac cggcgccctg
     1921 gcgctgctcg catgcgtgct gcgcgccaag cgcaccggaa agggctgcga cgtggacgcg
     1981 agcctgttcg acgtggccct gcatcagctc acctacatgg ggacctggca tctgaacggt
     2041 ggcaagccgt cctcgcgcca gccgcgcagc gcgcactact ccctcacgcc ggtgcagacc
     2101 ttcccgaccg ccgacggctg gatcttcgtg atgtgcatga aggaaagctt ctggtccgcg
     2161 ctgtgcgagg cgctgtcgcg cccggatctt ggccgggatg cgcgcttcgc gaccatggcc
     2221 gcgcggctgg agaatcgcgc cacgctgacc ggaatgctgg atgatgaatt tcgaagaaaa
     2281 accacggcac actggctcaa ggcgctttcg ggccgcctcc cggtcggccc ggtcaacgac
     2341 ctgcgcgcgg cgctggcgaa tcccttcgtg gccgcgaccg gcatggtgcg ctcgatcgag
     2401 caccccgaaa agcgcgggct gcgcgtgctc acccttccgg taaagatcga cggcgaacgg
     2461 ccgcagcccg tcgtgtgtga agggctgaag aaatgaagct tcaaggcatc cgcgtggtcg
     2521 atctctcgct tttccttccg ggtccctacc tcaccctgat acttgcggac cacggcgcgg
     2581 aagtgatgaa ggtcgagccg ccgggcgaag gcgatcccac gcgtcatctc ggaccgaagg
     2641 acggcggcga gacggtgtac ttccgcaacc tgaaccgcgg gaagaaaagt ctcgtcgtcg
     2701 acctcaagaa cccgcaggag cgcgaggcgc tgctcgagct ctgcgacacc gcggacgtct
     2761 tcgtcgagtc gttccgcccg ggcgtggctg atcgcctcgg ggtgggctac caggcgttgc
     2821 gcgcgcgcaa tccgcgcctc gtctactgct cgatcagcgc cttcg
//
LOCUS       DASUNT010000132         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_982348, whole genome shotgun sequence.
ACCESSION   DASUNT010000132 DASUNT010000000
VERSION     DASUNT010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982348"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2137
                     /locus_tag="VFX94_02640"
     CDS             <1..>2137
                     /locus_tag="VFX94_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5767106.1"
                     /translation="VVRIANARGGEGFVAVSEEARKRFWLDRARTAAIARHTNAFKIN
                     EDVVIPLDKLGEYNDAVERINIEFSIANKLALLDALEDYFSGSLGIRDDEVLGTRAAR
                     ALELLRRVRGRWQAIGADLDHYFDRLQSYAVRLSWKAEVRDELRRIFDGTAFRTVLEQ
                     AESIHERILRSRVFVALHMHAGDGNVHTNVPVNSDDYEMLQRASGAVARLMRLARSLG
                     GAISGEHGIGITKLEFLDPAELAAFRAYKQAVDPQGRFNAGKLLAGGDLANAYTPSFA
                     LIGAESLILEQTDLGRVAGAIKDCVRCGKCKPPCATHVPRANLLYSPRNKILAASLLI
                     EAILYEEQTRRGVALAHYDEFGDVADHCTVCHKCEPACPVDIDFGDVSIIMRNYLRRV
                     GKRRFNPGTQASMLFLNLTDPRAVKLVRTVMIDWGYRLQRFAHGLLKGFAREQTARPP
                     ASVGQRPLRTQVVHFVNKPMPGRLPKKTARALLDIEDPKVVPIVRDPARVTEDSDAVF
                     YFPGCGSERLFSQVGLATQAMLYTVGAQCVLPPGYLCCGYPQTAAGEAEKGERITVQN
                     RVLFHRLATTLNYLDIRTVIVSCGTCLDQLEKYDFGSIFPGARLLDIHEYLLEKGVKV
                     TDAPGVRYMYHDPCHTPIKTAKPLNVVNGLIGGEVRLTERCCGESGTLAMTRPDISTQ
                     VRFSKEESLRKGISALGEDGKRVKVLTSCP"
BASE COUNT          373 a          728 c          685 g          351 t
ORIGIN      
        1 gtcgtgcgca tcgcgaacgc gcgcggcggc gaaggcttcg tcgcggtgtc ggaggaggca
       61 cgcaagcgct tctggctcga ccgtgcgcgc accgcggcga tcgcacggca caccaacgct
      121 ttcaagatca acgaggatgt agtcatcccg ctggataagc tgggcgaata caacgatgcg
      181 gtggagcgca tcaacatcga gttttccatc gcgaacaagc tcgcgctcct cgacgcgctc
      241 gaggactact tttcgggcag cctcggcatc cgcgacgacg aggtcctcgg cacgcgggcg
      301 gcgcgcgcgc tggagctctt gcgccgcgtg cgcggccgct ggcaggcgat cggcgccgac
      361 ctcgaccatt acttcgatcg gctgcagtcc tacgccgtgc gcctgtcgtg gaaggccgag
      421 gtgcgcgacg agctgcgccg gatcttcgac ggaaccgcct ttcgcacggt cctcgagcag
      481 gccgaaagca tccacgagag gatcctgcgc agccgggtgt tcgtcgcgct gcacatgcat
      541 gccggggacg gcaacgtgca cacgaatgtc ccggtcaact ccgacgacta cgagatgctg
      601 cagcgcgctt cgggcgcggt cgcccggctc atgcgccttg cgcgctcgct gggaggcgcg
      661 atctcgggcg agcacggcat cggcatcacc aagctcgaat tcctcgaccc ggccgagctc
      721 gcggcgtttc gcgcctacaa gcaggcggtc gatccgcagg ggcgtttcaa cgcggggaag
      781 ctcctcgccg gcggcgacct cgccaacgcc tatacgccga gcttcgccct gattggcgcc
      841 gagtcgctta tcctcgagca gaccgacctc gggcgagtcg ccggcgcgat caaggactgc
      901 gtgcgctgcg gcaaatgcaa gcctccgtgc gcgacccacg tgccgcgcgc caacctgctc
      961 tactcgccgc gcaacaagat cctcgccgcc tcgctcctca tcgaggcgat cctctacgag
     1021 gagcagacgc gccgcggggt cgcgctcgcg cactacgacg agttcggcga tgtcgccgat
     1081 cactgcaccg tgtgccataa gtgcgagccc gcctgcccgg tggacatcga cttcggcgac
     1141 gtgtcgatca tcatgcgcaa ctacctgcgc cgcgtgggca agcgccgctt caaccccggc
     1201 acgcaggctt ccatgctgtt cctcaacctc accgacccgc gcgcggtgaa gcttgtgcgc
     1261 acggtgatga tcgactgggg ctaccggctg caacgcttcg cgcacgggct gctgaagggc
     1321 ttcgccagag agcagaccgc ccgcccgccc gccagcgtag ggcaaaggcc gcttcgcacg
     1381 caggtggtcc atttcgtgaa caagccgatg ccggggcggc tgcccaagaa aaccgcgcgc
     1441 gcgctcctcg acatcgagga tccgaaggtg gtgccgatcg ttcgcgaccc cgcccgggtg
     1501 acggaagact ccgacgcggt gttttacttc ccgggctgcg gatccgagcg gctcttttcg
     1561 caggtcggcc tcgcgacgca ggccatgctc tataccgtgg gcgcgcagtg cgtgctgccg
     1621 ccaggctacc tctgctgcgg ctacccgcag accgcggccg gcgaagccga gaaaggcgag
     1681 cgcattacgg tgcagaaccg agtgctgttc caccggctcg cgacaacgct gaactacctc
     1741 gacatacgca ccgtcatcgt ctcgtgcggt acttgcctcg atcagctcga gaagtacgac
     1801 ttcggctcga tcttcccggg cgcgcggctc ctggacattc acgagtacct gctcgaaaaa
     1861 ggcgtcaagg tgactgatgc gccgggtgtg cggtacatgt atcacgaccc ctgccataca
     1921 cccatcaaga cggcaaagcc gctcaacgtg gtgaatggcc tgatcggcgg tgaggtgcgc
     1981 cttaccgagc gctgctgcgg cgaatccggc acgctcgcca tgacgcggcc cgatatttcc
     2041 acgcaagtca ggttcagcaa ggaagaatcg ttgcgcaaag gaatatcggc tctgggagag
     2101 gacggcaagc gtgtaaaggt gctcacctca tgtccct
//
LOCUS       DASUNT010000133         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_18124, whole genome shotgun sequence.
ACCESSION   DASUNT010000133 DASUNT010000000
VERSION     DASUNT010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18124"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFX94_02645"
     CDS             <1..193
                     /locus_tag="VFX94_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767107.1"
                     /translation="LFARLMDGDDLAPLGAKAWGEARLPLEGEYVNVLTGERHEGRAG
                     HVGIAALLATFPAALLVRP"
     gene            209..643
                     /locus_tag="VFX94_02650"
     CDS             209..643
                     /locus_tag="VFX94_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767108.1"
                     /translation="MVRERPAGMTVGVHMCRGNAMGKWIAEGGYERIAEKSFSRMGVD
                     AFFLEFDSERAGGFEPLRFVSNGKRVVLGPVSTKTPALEDKDALKKKIDAAARFIALD
                     NLSISPQCGFASHEKGTMLSFREQEAKLRRVVETAMEVWGQA"
     gene            640..1731
                     /locus_tag="VFX94_02655"
     CDS             640..1731
                     /locus_tag="VFX94_02655"
                     /inference="COORDINATES: protein motif:HMM:NF042665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6607 family protein"
                     /protein_id="HEX5767109.1"
                     /translation="MKTFWIVLFALACGAARAAEPSQFIFAWPLDEKALKPRGATTRG
                     APVTLDAAPTESWKRLNEPGLGAQERDRRAILAMAGPYRVSFDFVEVVRYEPSLKQDA
                     PYQSWGTEYVFVTEDRSEFIALQHILVMRMLRDGDSGGSPIVSEPMVVRHWRQEWRYE
                     APTLLAYEGENTWARRATPAAARRGAWTQTVLQVDDSPRYAALGRWEHAKGMSTWISE
                     ETWRPLPRREFSVRKDYDVLVGTNRHTITPTGWVQEENNLKLAMKDRKYLAREYGVAR
                     YQRIKDYDFGAGEKYYTRTEPFWAEVRAAWRELEEGRGRFTARAPVDQAQLFLPFFRY
                     ADKLVEGAPFDRDDARAFVRRTLQDSYLK"
     gene            1760..2377
                     /locus_tag="VFX94_02660"
     CDS             1760..2377
                     /locus_tag="VFX94_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767110.1"
                     /translation="MKTLLTTIGLLVALSAQAQQKDMSFFITSVGPGKGADLGGLAGA
                     DAHCQKLAQTAGAGQREWRAYLSTDKVNARDRIGKGPWKNAKGEEIAKSVDDLHNDPK
                     INRQTGLDEKGSMVNGRGDSPNKHDILTGSTPDGRAIANKDLTCGNWAKSGEGAAMVG
                     HHDRIGLRDDDASKSWNSSHPSRGCSQDALKASGGAGLLYCFASN"
BASE COUNT          456 a          772 c          830 g          347 t
ORIGIN      
        1 cctcttcgcg cgcctgatgg acggcgacga ccttgcgccg ctcggcgcga aggcctgggg
       61 cgaggcgcgc ttgcctctcg aaggcgaata cgtcaacgta ctgaccggcg aacggcatga
      121 gggtcgcgcg ggtcacgtgg gcatagcagc gcttcttgcc acctttccag ccgcgctgct
      181 cgtgcggccg tgaacgcgcc gcccttccgt ggtgcgcgag cggccggccg gcatgacggt
      241 gggcgtgcac atgtgccgcg gcaacgccat gggtaagtgg atcgccgagg gcggctacga
      301 gcgcatagcg gaaaagtcct tttcccgcat gggggtcgat gctttctttc tcgagttcga
      361 cagcgagcgc gccggcggct tcgagccgct gcgcttcgtt tcgaacggca agcgcgtagt
      421 gctcggcccg gtttccacga agaccccggc gctggaagac aaggatgcat tgaaaaagaa
      481 gatcgacgcc gcggcgcgct tcatcgcgct cgacaacctc tcgatcagcc cgcaatgcgg
      541 ctttgccagc cacgagaaag gtacgatgct gtcattccgc gagcaggagg cgaagctgcg
      601 acgggtggtg gagaccgcga tggaggtatg gggacaggca tgaaaacgtt ctggatagtc
      661 ctttttgcgc tcgcttgcgg agcagccagg gcagctgagc cgagccagtt catcttcgcc
      721 tggccgctcg acgagaaggc gctcaagccg cgcggcgcga cgacgcgcgg tgcgccggtg
      781 acgctcgacg cggcgccgac agaatcctgg aaacggctga acgagcccgg cctgggcgct
      841 caagagcgcg atcgtcgcgc gatcctggcg atggccgggc cctaccgggt gagcttcgat
      901 ttcgtcgaag tagtgcgcta cgagccctcg ctcaagcagg atgcccctta ccagagctgg
      961 ggtaccgagt acgtgttcgt caccgaggac cggtcggagt tcatcgcact ccagcacatc
     1021 ctggtgatgc gcatgctgcg agacggggac agtggcggtt ctcccatcgt gagcgagcca
     1081 atggtggtgc gccattggcg tcaggagtgg cgctacgagg cgccgacgct cctcgcgtac
     1141 gagggggaga acacctgggc gcgccgcgcc acacccgccg ccgcgcgccg cggcgcctgg
     1201 acgcagacgg tgctccaggt cgacgactcg ccgcgctacg ccgccctcgg gcgctgggag
     1261 cacgcgaagg gcatgtcgac gtggatcagc gaagaaacct ggcggccgct gccgcggcgc
     1321 gagttcagcg tgcgcaagga ctacgacgtg ctcgtcggga cgaaccgaca caccatcacg
     1381 ccgacgggct gggtgcagga agagaacaac ctcaaactgg cgatgaagga caggaagtac
     1441 cttgcgcgcg aatacggcgt ggcccgatac cagcgcataa aggactacga cttcggcgcc
     1501 ggtgaaaagt attacacgcg caccgagccg ttctgggcag aggtgcgcgc cgcctggcgc
     1561 gagctcgagg aggggcgcgg ccgcttcacc gcacgcgcgc cggtggacca ggcgcagctc
     1621 ttcctgccgt tcttccggta cgccgacaag ctggtcgaag gcgcgccgtt cgatcgagac
     1681 gacgcgcgcg cctttgtccg acgcacgctc caggacagct acctgaaata aaggggcgag
     1741 gccccctgtt tacagcaaca tgaaaactct tcttacgacg atcggcctgc tcgtagcgct
     1801 cagcgcgcag gcgcagcaaa aggacatgag cttcttcatc accagcgtcg ggccgggcaa
     1861 aggggccgac cttggcggcc tcgcgggcgc ggatgcccat tgccagaagc tcgcgcagac
     1921 ggcgggggcc ggccagcgcg aatggcgggc ctacctcagc accgacaagg tcaatgcccg
     1981 cgaccggatc ggcaagggcc cgtggaagaa cgcgaaaggt gaagagatcg ccaagagcgt
     2041 ggacgacctg cacaatgacc cgaagatcaa caggcagacc ggcctcgacg agaaaggcag
     2101 catggtcaac gggcgcggcg acagcccgaa caagcacgac atcctgaccg gctcgacgcc
     2161 cgacggccgc gccatcgcga acaaggacct gacctgcggc aactgggcga agagcggcga
     2221 gggcgccgcc atggtcgggc atcacgaccg tataggcttg cgcgacgacg acgcctcgaa
     2281 gtcgtggaac tcctcgcatc cctcgcgcgg ctgcagccag gacgcgctga aggcgagcgg
     2341 cggcgccggt ttgctgtact gcttcgcgag caactgacgc agggcccatc cttcgagcca
     2401 cgccg
//
LOCUS       DASUNT010000134         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_281885, whole genome shotgun sequence.
ACCESSION   DASUNT010000134 DASUNT010000000
VERSION     DASUNT010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281885"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1728)
                     /locus_tag="VFX94_02665"
     CDS             complement(<1..1728)
                     /locus_tag="VFX94_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX5767111.1"
                     /translation="MSDVAAYSVTDGIAVITMNNPPVNGLGNALRAALMAHLKKAEAD
                     PAIKGAVIIGSAKAFSGGADIREFGKPREKPDLFEVNDQQDAMTKPLVAAIGGFALGG
                     GLELALGCHYRVALPRAQFGLPEVKLGILPGSGGTQRLPRIVPMAEAVGMMTSGATIP
                     AEKAKKLGLVDEIVTGDLLEGAMKFLQKPRAIRRIRDMKAKLDGEAKGFFAKVREQVA
                     KESRGYPAPLEIVACAEAAATLPFDEGRKVEREGFNRLISTPQSQALRHMFFAERQTS
                     KIPDVPEDTQVRPIKKAAVIGAGTMGGGIAMCFANAGIPVTVIETTQQALDKGLEKMK
                     ANYAATVAKGRMAQPEMDKRMALIHGTVNLAQAADADIVIEAVFERMDVKRDLLKKLD
                     AIVQKGAILATNTSTLDVDQIAAATTRPQDVIGTHFFSPANVMRLLEVVRGKKTAKDV
                     LATTMKLGKQLRKLPVVSGVCDGFIGNRMLEKYVQQSLFLLEEGATPAQVDGALRRWG
                     MAMGPFTMYDMAGNDIGWEIRKRRAAERPEMVYSKFADRICEQGWFGQKTGRGWYRYE
                     KGSRKPMVDP"
     gene            complement(1912..>2664)
                     /gene="bioB"
                     /locus_tag="VFX94_02670"
     CDS             complement(1912..>2664)
                     /gene="bioB"
                     /locus_tag="VFX94_02670"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX5767112.1"
                     /translation="VPDEEMLPLEDVIACAKTAKANGATRFCMGAAWRGPKDRDLEKV
                     AVFIREVKALGLETCVTLGMLKPGQAERLREAGLDYYNHNLDTSPQFYGEIISTRTYR
                     DRLETLERVRAAGIRVCYGGIVGMGESRADRAALVAQLASLDPQPESVPINSLIQVKG
                     TPLHGVDPIDPLEFVRTVAVTRICIPKAWVRLSAGRESMPEALQALCFLAGANSVFYG
                     DKLLTTPNPETLRDRALFERLGLRPTAAGAAD"
BASE COUNT          409 a          874 c          861 g          520 t
ORIGIN      
        1 gggatcgacc atgggcttgc ggctgccctt ctcgtagcga taccagccgc ggccggtctt
       61 ctgcccgaac cagccctgct cgcagatgcg gtcggcgaac ttcgaataca ccatctccgg
      121 gcgctcggcc gcgcggcgct tgcggatctc ccagccgatg tcgttgcccg ccatgtcgta
      181 catcgtgaac gggcccatcg ccatgcccca gcgccgcagc gccccgtcga cttgcgccgg
      241 cgtcgcgcct tcctcgagca ggaacaggct ctgctgcaca tacttctcga gcatgcggtt
      301 gccgatgaag ccgtcgcaca cgccggatac cacgggaagc ttcctgagct gctttccgag
      361 cttcatggtg gtggcgagca cgtccttcgc ggtcttcttg ccgcgcacca cctcgagcag
      421 ccgcatgaca ttcgcgggcg agaagaagtg cgtgccgatc acgtcctgcg ggcgtgtggt
      481 cgcagcggcg atctggtcga catccagtgt cgatgtattg gtagcgagga tcgcgccttt
      541 ctgcacgatg gcgtcgagct tcttcaaaag atcccgcttc acgtccatgc gctcgaacac
      601 cgcctcgatc acgatgtcgg cgtcggcggc ctgggcgagg ttgaccgtgc cgtggatcaa
      661 ggccatgcgc ttgtccatct cgggctgcgc catgcggcct ttcgccaccg tcgcggcgta
      721 gtttgctttc attttctcca ggcccttgtc gagcgcctgc tgcgtcgtct cgatcacggt
      781 gaccggaatg ccggcgttgg cgaagcacat cgcgatgccg ccgcccatgg tgcccgcgcc
      841 gatcaccgcc gccttcttga tcggccgcac ctgcgtgtct tccgggacgt ccgggatctt
      901 gcttgtctgg cgctcggcga agaacatgtg gcgcagggcc tgcgactgcg gagtgctgat
      961 gaggcgattg aaaccttcgc gctcgacctt tcggccttcg tcgaacggca gcgtggccgc
     1021 ggcctcggcg cacgccacga tctcgagcgg cgccgggtag ccgcgcgact ccttcgccac
     1081 ctgctcgcgc accttggcga agaacccttt cgcttcgccg tccagctttg ccttcatatc
     1141 ccggatacga cggattgccc ggggcttttg caggaacttc attgctcctt ccaggagatc
     1201 tccggtgacg atctcgtcca ccaggccgag cttcttcgcc ttctccgcag ggatggtggc
     1261 gccgctcgtc atcatgccca ccgcttccgc catcgggacg atgcgcggca ggcgctgggt
     1321 gccgcccgag ccgggcagga tgccgagctt cacttcgggc aggccgaatt gcgcgcgggg
     1381 gagcgcgacg cggtagtgac agccgagggc aagctcgagg ccgccaccga gcgcgaagcc
     1441 gccgatcgcc gcgacaagcg gcttcgtcat cgcgtcctgc tgatcgttca cttcgaaaag
     1501 gtcgggcttc tcgcgcggct tcccgaactc gcggatgtcg gcgccgccgg agaacgcttt
     1561 cgccgagccg atgatcaccg cgcccttgat cgccgggtcg gcctcggcct tcttgaggtg
     1621 cgccatcaga gcggcgcgca gcgcgttgcc gaggccgttg accggcgggt tgttcatcgt
     1681 gatgacggcg atcccgtcgg taacggagta ggctgcaacg tcgctcattt gggaaagacc
     1741 gggaaagggg ggcgagccga ttttactggc aaagtcagcg cgcgttcatc gccggttcgt
     1801 cgggcgaagc gcatgagcgg acctcgcggg cggtggtctg ataggttaac ttatggttgt
     1861 ctaaccggaa cccggaagcc cggccggtac atcctcgcga cgcgttgagc ttcagtccgc
     1921 cgcgcctgca gcggtcgggc gcaatcccag gcgctcgaaa agcgcgcggt cccggagcgt
     1981 ttccgggttc ggggtagtga ggagcttgtc gccgtagaac accgagttcg cgcctgcgag
     2041 gaagcatagc gcctgcagtg cctcgggcat gctttcgcgc ccggcggaga gtcgcaccca
     2101 agccttgggt atgcagatgc gcgtcaccgc aacggtgcgg acaaattcga gcggatcaat
     2161 aggatcgacg ccgtgaagcg gcgtgccttt gacttgaatc aatgaattga tgggaacgct
     2221 ttcgggttga gggtcgaggc tcgcgagctg cgcgacgagc gcggcgcgat ccgcgcgcga
     2281 ttcgcccatt cccacgatgc cgccgtagca cacgcggatg ccggcggccc gtacgcgctc
     2341 gagcgtctcc aggcggtcgc ggtaggtgcg cgtgctgatg atctcgccgt agaactgcgg
     2401 cgaggtgtcc aggttatggt tgtagtagtc gagccctgct tcgcgcagcc gctcggcctg
     2461 gccgggtttc agcatgccga gagtcacgca ggtttccagc cccagcgcct ttacctctct
     2521 aataaagaca gcaactttct cgagatccct gtccttcgga ccacgccatg ccgcgcccat
     2581 gcagaagcgt gttgcaccat tcgcctttgc ggtctttgcg caagcgatga catcctccag
     2641 gggcagcatt tcctcatccg ggac
//
LOCUS       DASUNT010000135         2064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_632651, whole genome shotgun sequence.
ACCESSION   DASUNT010000135 DASUNT010000000
VERSION     DASUNT010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632651"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /gene="murB"
                     /locus_tag="VFX94_02675"
     CDS             <1..313
                     /gene="murB"
                     /locus_tag="VFX94_02675"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF014872.4"
                     /note="catalyzes the reduction of UDP-N-acetylglucosamine
                     enolpyruvate to form UDP-N-acetylmuramate in peptidoglycan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX5767113.1"
                     /translation="RAMMKELLARRIATQPLSLPNAGSVFRNPPKHHAACLIESCGLK
                     GFERGGARVSEKHANFIVNPKGAAKAADIEWLIRHVQRMVYEMKGVRLAPEVRIVGEE
                     E"
     gene            310..1221
                     /locus_tag="VFX94_02680"
     CDS             310..1221
                     /locus_tag="VFX94_02680"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX5767114.1"
                     /translation="MNFGKVAVLMGGKSAEREISLMSGNGVLKALRSRGIEAHAFDPA
                     QRDLFDLKQEAYARCFIALHGRGGEDGTIQGALEVLGIPYTGSGVLGSAVGMDKVRTK
                     MIWIANGLPTPRYRILKEHDNWHAVARGLKLPLIVKPANEGSTLGLTKVRLVKELPAA
                     YELASKKYHDIALAEAFVDGPEYTASILGDTALPLIRIEAPQGNYDYQHKYFTDDTKY
                     HCPCGLPAKKEQELQALSLEAFRVAGCSGWGRIDLMLDAKKRPWLLEVNTSPGMTGHS
                     LVPMAARAVGISYEDLCVQILELAHVG"
     gene            1211..1909
                     /locus_tag="VFX94_02685"
     CDS             1211..1909
                     /locus_tag="VFX94_02685"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEX5767115.1"
                     /translation="MSARLTLIAGILSGLAMLAACLGALYWLLLPQRFPVTQLDIRGE
                     LRNASRAEIERALPRAAGNFFAADLAAVRDRVEQLPWVRRVAVRRVWPARLEITVEEH
                     VALARWGNDRLVNTFGETFSAHTTQALPAFVAPGGTAAEVVRRYRRFNQLVAPLGTSV
                     ERVVLTQRLAWQLRLGNGLHIMLGRDADLAEERLRKFVEVHPATLARIAKKHEYVDLR
                     YPNGFALRLTDGSS"
     gene            1916..>2064
                     /locus_tag="VFX94_02690"
     CDS             1916..>2064
                     /locus_tag="VFX94_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX5767116.1"
                     /translation="MPKDNKNLIVALDIGTSKVACLVAELGADGALALLGMGNHDSKG
                     LKKGVV"
BASE COUNT          371 a          669 c          706 g          318 t
ORIGIN      
        1 gcgcgcgatg atgaaggagc ttctcgcccg gcggatcgcc acccagccgc tttcgctccc
       61 caacgcaggc agcgtgttcc gcaatccgcc caagcaccac gccgcgtgcc tcatcgaatc
      121 gtgcggcctc aagggtttcg agcgcggcgg cgcgcgcgtg tcggagaagc acgcgaactt
      181 catcgttaac ccgaagggcg cggccaaggc ggcagacatc gaatggctga tccgccatgt
      241 ccagcgcatg gtctacgaga tgaagggcgt ccgcctcgcc cccgaagtgc gcatcgtggg
      301 ggaggaagaa tgaacttcgg caaggttgcc gtgctgatgg gagggaaatc ggccgagcgg
      361 gagatctcgc tcatgagcgg caacggcgtg ctgaaggcgc tgcgctcgag aggaatcgag
      421 gcgcacgcct tcgaccctgc gcagcgcgac ctcttcgatc tcaagcagga agcgtatgcg
      481 cgctgcttca tcgcgctgca cgggcgcggc ggcgaggacg gcacgatcca gggcgcgctc
      541 gaggtcctgg gcattcccta caccggaagc ggcgtgctcg gctcggccgt cggcatggac
      601 aaggtgcgta ccaagatgat ctggatcgcg aacgggctgc cgacgccgcg ataccggatt
      661 ctcaaggagc acgacaactg gcacgccgtg gcgcgcggcc tgaagttgcc gctcatcgtg
      721 aagcccgcga acgagggctc gaccctcggg ctcacgaaag tgcgcctggt taaggaattg
      781 ccggcggcgt atgagctggc atcgaagaaa taccacgaca tcgccctcgc cgaggcgttc
      841 gtcgatgggc cggaatacac ggcgagcatc ctcggcgata ccgccctgcc gctgatccgc
      901 atcgaagcgc cgcagggtaa ctacgactac cagcacaaat actttaccga cgatacgaag
      961 taccactgcc cctgcggcct gccggcgaag aaagagcagg agctgcaggc tttatcgctc
     1021 gaggcattcc gcgtcgccgg atgcagcggc tggggacgca tcgacctcat gctcgacgcg
     1081 aaaaagcggc catggctgct ggaagtgaac acctcgccgg gaatgaccgg acacagcctg
     1141 gtgccgatgg cggcacgcgc ggtcggcatc tcgtacgaag acctgtgcgt gcagatcctg
     1201 gagcttgcgc atgtcggcta gattgacgct catcgcggga atcctttccg ggctcgcgat
     1261 gctggcggcg tgtctggggg ccctctattg gctgcttctg ccgcagcgct ttcccgtcac
     1321 gcagctcgac atcaggggag agctcaggaa cgcgagccgc gccgagattg agcgcgcgct
     1381 gccgcgcgcg gcgggaaact tcttcgctgc cgatctcgcc gcagtgcgcg accgcgtcga
     1441 gcagctgccg tgggtgcgcc gcgtcgccgt gcgccgcgtg tggccggccc ggctcgagat
     1501 cacggtggaa gagcacgtcg cgctggcgcg ctggggcaac gaccggctcg tcaatacttt
     1561 cggcgagacc ttctccgccc acacgacgca agcactgccc gcgttcgtcg ccccgggcgg
     1621 taccgcggcc gaggtcgtgc gccgttaccg acgcttcaac cagctggtgg cgccgctcgg
     1681 cacgagcgtc gagcgcgtgg tgctgacgca gcgcctcgcc tggcagcttc gcctgggcaa
     1741 cgggctgcac atcatgctcg ggcgcgacgc cgacctggcg gaggagcggc tgaggaagtt
     1801 cgtggaggtc cacccggcga cgcttgcccg gatcgcgaag aagcacgagt acgtcgatct
     1861 gcgctacccg aacggcttcg cgttacgcct gaccgacggg agcagctgac aaaggatgcc
     1921 caaggacaac aagaacctca tcgtggcgct cgacatcggc acctccaagg tcgcgtgtct
     1981 ggtggccgag ctgggggcgg acggcgcgct cgcgctgctc ggcatgggca accacgactc
     2041 caaaggcctg aagaagggcg tggt
//
LOCUS       DASUNT010000136         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1158970, whole genome shotgun sequence.
ACCESSION   DASUNT010000136 DASUNT010000000
VERSION     DASUNT010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158970"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1577
                     /locus_tag="VFX94_02695"
     CDS             <1..1577
                     /locus_tag="VFX94_02695"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEX5767117.1"
                     /translation="FRKPPPELLQLPPVKPPEADRAPTALRVMVKKINITGNTVFKAG
                     ELESIAAPFENREVTSTDLEELRQRLTQHYINAGYINSGAVIPDQKVEDGVIEIRIVE
                     GRLTRTEIEGTVHFSKDYFSDRIALSSGPPLNLRELEQRLQVLLTNPMVASINAQVVP
                     GERPGEAVLRAQVQEAPRYELSAVLDNKLAPSLGEAKLTLLGAVNNLTGRGDQLGAEF
                     GYAGEGIPHDARLRYRTPISARDTALGVHYENTKAVVQEEPFDVLDITSKLETVGVEL
                     SHPVYRTQNRQLVLSGLVERRETETTLLGVPFSFSPGVQDGKATVSVLRLIADYLDRG
                     RTQVIAARSTLNIGLDAFDSTINTDGSPDSRFVSWLGQLQYVRRLSERRGDQLRLRAD
                     LQLTDDALLPVEQYAVGGLDSVRGYRSFQLLRDYGYTASIEYRIPVFSNPVGARNLLF
                     AAYVDTGGAKFKERDNPSPDNLTGVGIGFIWNPVREFFAELYYAEGLDDVPAPASSHL
                     QDESLYFRLVYRPSWF"
     gene            complement(1626..>2052)
                     /locus_tag="VFX94_02700"
     CDS             complement(1626..>2052)
                     /locus_tag="VFX94_02700"
                     /inference="COORDINATES: protein motif:HMM:NF017829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF928 domain-containing protein"
                     /protein_id="HEX5767118.1"
                     /translation="STESLQAAATPVLEITLQPPLAKGIHAFRLADHGVALKPGVEYQ
                     WFVAVVRNPAQRSNDVLAGGTIKRVTDSPVPAQLKQASPAQQAALYAEAGIWYDAIDQ
                     LSRQISADQSNRQLRERRAALLEQVGLREAAAFDRSPGS"
BASE COUNT          345 a          700 c          684 g          323 t
ORIGIN      
        1 agttccgtaa gccgccgccc gagctgttgc agcttccccc ggtcaagccg cccgaggcag
       61 accgagcgcc caccgcgctg cgcgtgatgg tgaagaagat caacatcacg gggaacacgg
      121 tgttcaaggc gggggagctg gagagtatcg cggcgccctt cgagaaccgc gaggtgacga
      181 gcacggatct cgaagagctg cgccagcgcc tcacccagca ctacatcaat gcgggctaca
      241 tcaactccgg cgcggtgatc ccggaccaga aggtcgagga cggcgtgatc gagatccgca
      301 tcgtcgaggg gcggctcacg cgcaccgaga tcgagggcac ggtgcacttc agcaaggact
      361 atttcagcga ccgcatcgcg ctctcctccg gtccgccgct caacctgcgg gagctcgagc
      421 agcggctcca ggtgctgctc accaatccga tggtcgcgag catcaacgcg caggtcgtgc
      481 cgggcgagcg cccgggggag gcggtgctga gggctcaggt gcaggaggcg ccgcgctatg
      541 agttgagcgc cgtgctcgac aacaagctcg cgccgagcct cggggaggcg aagctcaccc
      601 tgctcggcgc ggtgaataac ctcaccggcc ggggcgatca gctcggcgcg gagttcggct
      661 acgcggggga gggcatcccg cacgacgcgc ggcttcgcta tcgcacgccg atcagcgcgc
      721 gcgacacggc gcttggcgtg cactacgaga acaccaaggc cgtggtgcag gaagagccct
      781 tcgacgtgct cgacatcacg agcaagctcg aaaccgtcgg agtggagcta agccaccccg
      841 tctatcggac gcagaaccgc cagctcgtgc tctcaggcct cgtcgagcgc cgcgagaccg
      901 aaaccacgct cctcggcgtg ccgttctcgt tctcgcccgg cgtacaggat ggcaaggcga
      961 ccgtatcggt gctgcggctg atcgccgact acctcgatcg cggccgcacg caggtgattg
     1021 cggcgcgttc cacgctcaac atcgggctcg atgcgttcga ctcgaccatc aacaccgatg
     1081 gctcgcccga cagcaggttt gtctcctggc tcggccagtt gcagtacgta cggcgcttga
     1141 gcgagcgacg cggcgaccag cttcgcctgc gcgcggacct gcagctcacc gacgatgcgc
     1201 tgctgccggt cgagcagtac gcggtcggag gactggacag cgtgcgcggc tatcgcagct
     1261 tccagctcct tcgcgactac ggctacaccg cctcgatcga gtaccgcatc ccggtgttca
     1321 gcaatcccgt gggtgcgcgt aaccttctct tcgcggcgta cgtcgacacc ggcggcgcga
     1381 agttcaagga acgggacaac ccctcgcccg acaacctcac cggcgtgggg atcggcttca
     1441 tctggaaccc ggtgcgcgag ttcttcgcgg agctctacta cgccgagggg ctcgacgacg
     1501 tgcccgcccc ggccagcagc cacctgcagg acgaaagcct ctactttcgc ctggtgtacc
     1561 ggccgtcgtg gttctaaaga aaaacgaggc ccccgccttc gcgggggcga ctgttcagac
     1621 gttctctagc tgcccgggct gcgatcgaac gcggccgctt cgcgcaatcc cacctgctca
     1681 agcagcgcgg cgcgacgctc gcgcagctga cggttcgact ggtcggcgct gatctgcctg
     1741 gagagctggt cgatcgcgtc gtaccagata cccgcctcgg catagagcgc ggcctgctgc
     1801 gcaggcgatg cttgcttgag ctgcgccggc accgggctgt cggtcacccg cttgatcgtg
     1861 cctccggcga gcacgtcgtt cgagcgctgc gcggggttgc gcacgaccgc gacgaaccac
     1921 tggtactcga cgccgggctt gagcgccacg ccgtggtcgg ccagccggaa cgcgtgaatg
     1981 cctttcgcga gcggcggctg cagcgtgatc tcgagcacgg gcgtcgccgc ggcctgcagg
     2041 ctctcggtgc tg
//
LOCUS       DASUNT010000137         3419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1247119, whole genome shotgun sequence.
ACCESSION   DASUNT010000137 DASUNT010000000
VERSION     DASUNT010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247119"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VFX94_02705"
     CDS             complement(<1..828)
                     /locus_tag="VFX94_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5767119.1"
                     /translation="MPKRQIRYAVVGLGHIAQVAVLPAFANARRNSRLAALVSGDAVK
                     LKQLSQKYGVEHAFTYEQYDACLKSVDAVYIALPNQLHCDYAVRAARAGVHVLVEKPM
                     AVTEEECERMIGAAREAGVKLMVAYRLHFERANLEAGEIVRSGRLGEARLFNSTFCTP
                     VVPGNIRVRRDAGGGVLYDIGIYCINAARALFGDEPVEVRATTAGMLDEVEESVSAQL
                     TFPNERIAAFTASFGAAKVSAYRIAGTKGDLAVEPAYDYARPLRHRLTIDGEMRERRF
                     "
     gene            complement(821..1027)
                     /locus_tag="VFX94_02710"
     CDS             complement(821..1027)
                     /locus_tag="VFX94_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767120.1"
                     /translation="MATCEVCGNSYDKAFSVRMPDGAAHTFDSFECAIAKLAPSCAHC
                     SCRVIGHGVERDGKIFCCEHCAHA"
     gene            complement(1050..1868)
                     /locus_tag="VFX94_02715"
     CDS             complement(1050..1868)
                     /locus_tag="VFX94_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX5767121.1"
                     /translation="MARGLWKGAISFGLVNVPVELHSAKKHSTELDMTMLDKRDLAPV
                     GYKRVNKSSGNEVPWDDVVKGYEYEDDKYVVLSDEDFRRANPEAAKSVDIQAFVNLVD
                     IAPQYFETPYYLIPGKRGEKAYALLRDSLRKAGKAGIASVVIRTRQHLAALVPQGDIL
                     VLNTLRYADELKEPSELKVPAAKVSAKELDMAMRLVEDMADEWKPERYRDTFRDDLLK
                     RIEEKVKAGQTEELTEPEKERKAEKAEVIDLMDLLKKSIERKPAKRAARKRRAA"
     gene            complement(1920..2435)
                     /locus_tag="VFX94_02720"
     CDS             complement(1920..2435)
                     /locus_tag="VFX94_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767122.1"
                     /translation="MKPKLTVVALGLAFGLASAVQAQTPPPAKGDSAAPRAEQSDRKA
                     KNSAEDRIEQEYKAAREKCNSMEGNAKDICMAEAKGKQKVAKAELEAKDAKDPAKAQR
                     KVADARADAAYEVAKEKCDEQKGEAKDACMKQARVERDRAKGKAEKTAEAKQEKSRAA
                     SGSTAPEKQQK"
     gene            complement(2468..2632)
                     /locus_tag="VFX94_02725"
     CDS             complement(2468..2632)
                     /locus_tag="VFX94_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010120894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HEX5767123.1"
                     /translation="MLSWALAFFIIAIIAAVFGFTGIAAGAAEVAKILFVVFVVLFLV
                     SLVAGLLRRP"
     gene            2838..>3419
                     /locus_tag="VFX94_02730"
     CDS             2838..>3419
                     /locus_tag="VFX94_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5767124.1"
                     /translation="MQPDDNFPFMTWRARPDMSCEYVKREWLRDTGYTMEQAAGQGWT
                     RCLHAEDLARWLDVCVSAFDRREPFEIEYRLRRRDGEYRWVLERALPRYSAGELFLGF
                     GGVCVDIDTQKRESEGLARELERERRLRLTTEEASRLRQGLMISVLQELNLPSRAIAT
                     WAAHLRGQMPRAAALEAIERSARAQSRIIATLLE"
BASE COUNT          471 a         1174 c         1127 g          647 t
ORIGIN      
        1 gaagcgccgc tcgcgcatct cgccgtcgat cgtcagccgg tggcgcagcg ggcgcgcgta
       61 gtcgtaggcg ggctcgaccg cgagatcgcc cttggtgccg gcgatgcggt acgcggacac
      121 cttggcggcg ccgaagctcg cggtgaaggc ggcgatgcgc tcgttgggga aggtgagctg
      181 cgcgctcacc gactcctcca cctcgtcgag catgcccgcc gtggtggcgc gcacctccac
      241 cggctcgtcg ccgaacaggg cgcgcgccgc gttgatgcag tagatgccga tgtcgtagag
      301 cacgccgccg ccagcgtcgc ggcgcacgcg gatgttgccc gggacgaccg gggtgcagaa
      361 cgtggagttg aagaggcgcg cctcgccgag gcggcctgag cgcacgatct cgccggcctc
      421 gaggttggcg cgctcgaagt gcaggcggta ggcgaccatc agcttgacgc cggcctcgcg
      481 cgccgcgccg atcatgcgct cgcactcctc ttcggtcacc gccatcggct tctcgaccag
      541 cacatggacg ccggcgcgcg cggcgcgcac ggcgtagtcg cagtgcagct ggttgggcag
      601 cgcgatgtac accgcgtcga cgctcttcag gcaggcgtcg tactgctcgt aggtgaaggc
      661 gtgctcgacg ccgtacttct gcgagagctg cttcagcttc accgcgtctc cggagacgag
      721 cgcggcgagc ctggagttgc gccgggcgtt ggcgaacgcg ggcagcaccg cgacctgggc
      781 gatgtggccc aggccgacca ccgcgtagcg gatctgccgc ttaggcatgg gcgcagtgct
      841 cgcagcagaa gatctttccg tcgcgctcga cgccgtggcc gatgacgcgg cagctgcagt
      901 gcgcgcagct cggcgcgagc ttcgcgatcg cgcactcgaa gctgtcgaag gtgtgcgcgg
      961 ccccgtcggg catcctcacc gagaacgcct tgtcgtagct gttgccgcag acttcgcacg
     1021 tcgccatgtc gcttcctttc ggtttcgttt cacgccgcgc ggcgctttct cgcggcgcgc
     1081 ttcgcaggct tcctctcgat gctcttcttc aggaggtcca tcagatcgat cacctccgct
     1141 ttctcggcct tgcgttcctt ctcgggctcg gtgagctcct cggtctggcc ggccttcacc
     1201 ttctcctcga tgcgcttcag gaggtcgtcg cggaaggtgt cgcggtagcg ctcgggcttc
     1261 cactcgtcgg ccatgtcctc gaccaggcgc atcgccatgt cgagctcctt ggccgagacc
     1321 ttcgccgcag gcaccttgag ctcggagggc tccttcagct cgtcggcgta gcgcagcgtg
     1381 ttcagcacca ggatgtcgcc ctgcggcaca agcgccgcga ggtgctgccg ggtgcggatc
     1441 accacgctcg cgatgccggc cttgccggcc ttcctcagcg agtcgcgaag cagcgcatat
     1501 gccttctcgc cgcgcttgcc cgggatcagg taatagggcg tctcgaagta ctgtggcgcg
     1561 atatcgacca agttcacgaa agcctggata tcgacgctct tcgcggcctc cgggttggcg
     1621 cgccggaagt cctcgtcgga cagcaccacg tacttgtcgt cctcgtactc atagcccttg
     1681 acgacgtcgt cccacggcac ttcgttgccg ctcgacttgt tgacgcgctt gtagcccacc
     1741 ggggcgaggt cgcgcttgtc gagcatcgtc atgtcgagct cggtcgagtg cttcttcgcc
     1801 gagtgcagct cgacgggcac gttgaccagc ccgaagctga tcgctccttt ccacagccct
     1861 cgcgccatcg ctaccgccgg ggcgttcctg ccgcttcgag caggaacgct ccgggctcgc
     1921 tacttctgct gcttctcagg tgcggtgctc ccgctcgccg cgcggctctt ctcctgcttt
     1981 gcctcggcgg tcttctccgc cttgcccttg gcgcggtcgc gctccacgcg cgcctgcttc
     2041 atgcacgcgt ccttcgcctc gcctttctgc tcgtcgcact tctccttggc aacctcgtag
     2101 gcggcgtcgg ccctggcgtc ggcgaccttg cgctgcgcct tggccggatc cttggcgtcc
     2161 ttcgcctcga gctcggcctt cgcgaccttc tgcttgccct tcgcctcggc catgcagatg
     2221 tccttggcgt tgccttccat gctgttgcat ttctcgcgcg cggccttgta ctcctgctcg
     2281 atgcgatctt cggcgctgtt tttcgccttg cgatcggact gctcggcgcg tggcgcagcg
     2341 gaatcgccct tggccggggg cggggtttgc gcctgcacgg cgctcgccag gccgaatgca
     2401 aggcccagcg cgacaacggt caacttcggc ttcatgatgc gtatctctcc tctgggtttg
     2461 tgtgcactca aggccttctc agcagtcccg cgacgagcga gaccaggaaa aggaccacga
     2521 acacgacgaa aaggatcttg gcgacctcgg ccgctccggc agcgatgccg gtgaagccga
     2581 ataccgccgc gatgattgcg atgatgaaaa acgcaagcgc ccaactcagc atgacggctc
     2641 ctcgataaaa gtggttgcag cacgggatgc ccagcaaagt ccgtgccggg cgcagagcat
     2701 gcgcgcctgc gccgcaccgc ttgtaacgtt cggttaggga tctgtaagaa ctacgtgtgg
     2761 cgcggctgcg tcctacgtag tgaaagcgct acgggcacgg ctcctgctga tgctcattcg
     2821 agcttgttcc gcgcggcatg caacccgacg acaacttccc gttcatgaca tggcgcgcgc
     2881 gccccgacat gtcgtgcgag tacgtgaagc gcgagtggct tcgagacacg ggttacacga
     2941 tggagcaggc cgcgggacag ggctggacac gctgcctgca cgccgaggac ctggcgcgct
     3001 ggctcgatgt gtgcgtgtcc gccttcgacc ggcgcgagcc cttcgagatc gagtaccggc
     3061 tgcgacggcg cgacggcgaa taccgctggg tgctggagcg tgcgctgccg cgctattcgg
     3121 ccggggagct cttcctcggc ttcggcggcg tgtgcgtcga catcgacacc cagaagcgcg
     3181 aaagcgaggg cctcgcgcgc gagctcgagc gcgagcggcg cctgcgcctc acgaccgagg
     3241 aggcgagccg gctgcgccag ggtctgatga tctcggtgct gcaggagctg aatctcccct
     3301 cgcgcgccat tgccacctgg gcggcgcacc tgcgcggcca gatgccgcgc gctgcggcgc
     3361 tcgaggcgat cgagcgcagc gcccgcgccc agagccggat catcgccacg ctgctcgag
//
LOCUS       DASUNT010000138         3057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_632963, whole genome shotgun sequence.
ACCESSION   DASUNT010000138 DASUNT010000000
VERSION     DASUNT010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632963"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1935)
                     /locus_tag="VFX94_02735"
     CDS             complement(<1..1935)
                     /locus_tag="VFX94_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEX5767125.1"
                     /translation="MDARGARPLAAEAVLTDGFIWALASLCRLHRVPFDAQLVLKQFA
                     PPYDLATLLRAAQACGLKAGLAGARADALESLPLPCLGFVRPAAQPANDPAPPTPALL
                     VRADAERLLYFLAGENAPRTLARGEFAARFEQTVLLAAPAPPAPDDADGADKRAFGLR
                     WFVRELVRHRAIWRDVLLASAAIHIIGLATPLFTQVVIDKVVVHHTSSTLIAIAIGLG
                     MFLVFNAAMTWMRQYLVLHTGNRADAVLATQVFSHLFRLPLAYFEHRPTGTTVARLHG
                     VETIREFITGAAISFVLDLPFLLVIIAVMFFYSWQLSFIALGIVFLLAITSLLVTPVL
                     REKLNAQFLLGARNQAFVTEYVSGVETVKSLQFEPVLERRYGEQLSAYLGASFATRNL
                     ANSYNVVANSLEQLMTLGILVAGAILVMRSDGFTIGMLVAFQMLAARMAQPMLRIAGL
                     WQEFQQASIAVKRLGDIMNAPAEPYSLAPSRSTGRAGAIALQGVSFRYSERHPYLFRN
                     VDFAIRPGELILVSGPSGSGKSTLARLLLGFYRPTDGRILLDSVQLDHLCANELRLLY
                     GVVPQETQLFSGSVYDNLAMANPHASFEEILAACRSAEIHDTIERLPQGYQTSLGEHG
                     VGLSGGQKQRLAIARALLKRP"
     gene            complement(2013..2651)
                     /locus_tag="VFX94_02740"
     CDS             complement(2013..2651)
                     /locus_tag="VFX94_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5767126.1"
                     /translation="MHALVIDDHPLIQEIVPALLAKALGEVAVATESTLEAGIARASR
                     EKMPELVVLDLGLPGCQGLEALARFRSRFPDVPLVVLSGITDRGSIVAALEAGAVGYI
                     PKTSKPDVMIAALKVVAAGGTYVPPEALEDEGEGERPAYRRRTAALDLTQRQKQVLRL
                     ILKGYNNDRIASELSIAPNTVKQHAHAIFTSLGVSTRAEAVVAAARHGLSLG"
     gene            complement(2662..>3057)
                     /locus_tag="VFX94_02745"
     CDS             complement(2662..>3057)
                     /locus_tag="VFX94_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX5767127.1"
                     /translation="CLRVANAAGAHAVIAPRDRAAGITPVVSKVASGAAENTPYLMVT
                     NLARTLGEVKEHNIWVVGADERADKTLYEADLPRSIAWVLGAEGEGMRRLTRESCDVL
                     VRIPMRGEVQSLNVSVAAGICLFESVRRR"
BASE COUNT          538 a          947 c         1102 g          470 t
ORIGIN      
        1 cggccgcttg aggagcgcgc gcgcgatggc gaggcgctgc ttctgtccgc cggacaggcc
       61 gacgccgtgc tcgccgaggg acgtctggta gccctgcgga agtctctcga tggtgtcgtg
      121 gatttcggcc gagcggcagg cggcgaggat ctcttcgaag ctcgcgtgcg ggttcgccat
      181 tgccaggttg tcgtacacgc tgcccgagaa aagctgcgtc tcctgtggca cgacgccgta
      241 gagaagccgc agctcgttgg cgcagaggtg atcgagctgc accgaatcga gcaggatgcg
      301 gccgtccgtt gggcggtaga agccgaggag gagcctggcg agggtgctct tgccgctgcc
      361 cgagggaccg ctcacgagga tgagctcgcc gggcctgatt gcgaagtcga cgttgcggaa
      421 gagatacggg tgacgctcgc tgtagcggaa ggaaacgccc tgcagggcga tcgcgccggc
      481 gcggccggtg gagcgcgacg gggcgagcga gtagggctcg gcgggcgcgt tcatgatgtc
      541 gcccaggcgc ttcaccgcga tgctcgcctg ctggaattcc tgccacaggc ccgcgatgcg
      601 cagcatcggc tgcgccatgc gcgcggcgag catctggaag gcgaccagca tgccgatggt
      661 gaagccgtcg cttcgcatga cgaggatcgc gccggcgacg aggatgccga gcgtcatgag
      721 ctgctcgagc gagttcgcga cgacgttgta gctgttcgcg agattgcggg tggcgaagct
      781 cgcgccaagg taggccgaga gctgctcgcc gtagcggcgt tcgagcaccg gctcgaactg
      841 gagcgacttg accgtctcga cgcccgagac gtactcggtg acgaaagcct ggttgcgcgc
      901 gccgaggagg aactgcgcgt tgagcttttc gcgcaatacc ggcgtcacca gcaggctggt
      961 gatcgccagg agaaaaacaa tgcccagggc gatgaaggaa agctgccagc tgtagaagaa
     1021 catgaccgcg atgatcacca ggaggaacgg caggtcgagc acgaaggaga tcgccgcgcc
     1081 ggtgatgaac tcgcggatgg tctccacgcc gtgcaggcgc gccaccgtgg tgccggtcgg
     1141 gcggtgctca aagtaggcga gcggcagccg gaagaggtgg ctgaacacct gcgtcgcgag
     1201 cacagcgtcg gcgcggtttc ccgtgtgcag cacgagatat tggcgcatcc aggtcatcgc
     1261 ggcgttgaag acgaggaaca tgcccaggcc gatggcgatg gcgatgaggg tgctgctcgt
     1321 gtggtgaacc accaccttgt cgatcaccac ctgagtgaag agcggcgtcg cgaggccgat
     1381 gatgtggatg gcagccgagg cgaggagtac gtcgcgccag atggcgcggt ggcgcacgag
     1441 ctcgcgtacg aaccagcgca atccgaacgc gcgcttgtcg gcgccgtcgg cgtcgtcggg
     1501 cgcgggcggc gccggcgcgg cgagaagcac tgtctgctcg aagcgcgcgg cgaattcgcc
     1561 gcgcgccagc gtgcgcggcg cgttctcgcc ggcgaggaag tagagcaggc gctcggcgtc
     1621 ggcgcgcacg aggagcgccg gcgtgggcgg cgccgggtcg tttgccggct gcgctgcggg
     1681 gcgcacgaag ccaagacagg gcagcggcag gctttcgagc gcgtcggcgc gcgcgccggc
     1741 aaggccggcc ttcaggccgc aggcctgcgc cgcgcgcagc agcgtcgcga ggtcgtaggg
     1801 cggcgcaaac tgcttgagga cgagctgcgc atcgaaggga acgcggtgca aacggcaaag
     1861 gctcgccagg gcccagatga agccgtctgt gagcactgct tcggcggcaa gcggccgcgc
     1921 cccccgcgcg tccattggaa gcctccccgc cgacaagcta ctccaacgac ggagaaagga
     1981 aatactccgt gagttctaca acccggttgt actcagccga gcgaaaggcc gtgacgcgcc
     2041 gcagcgacaa ccgcctcggc gcgagtcgac actcctaggc tggtgaagat cgcgtgggca
     2101 tgctgcttca ccgtgttcgg ggcgatcgac agctccgagg cgatgcgatc gttgttgtat
     2161 cccttgagga tgagccgcag tacctgcttc tggcgctgcg tgagatcgag cgcagccgtg
     2221 cggcggcggt acgccggccg ttccccttcg ccttcgtcct cgagcgcctc cggcggcaca
     2281 tacgttccgc ctgccgccac caccttgagg gcggcgatca tcacgtcggg cttcgaggtc
     2341 tttggaatgt agccgacggc gcctgcttcc agcgcggcca cgatggaccc gcgatcggta
     2401 atgccggaaa gcacgacgag cggaacatcc ggaaacctgg agcggaaacg cgcgagagcc
     2461 tcgagcccct ggcagccggg caggccgagg tcgagaacga ccagctccgg catcttctcg
     2521 cgcgaggcgc gcgcgatgcc ggcttcgagc gtcgattcgg tggcgacggc gacttcgccg
     2581 agcgccttgg cgagaagcgc gggcacgatt tcttggatca acgggtgatc gtcgatgacg
     2641 agggcgtgca tgcgcgaaat gttacctccg gcgcaccgac tcgaacagac aaattccggc
     2701 cgcaacagag acgttcaggc tttggacttc gccgcgcatc gggatgcgca cgagaacatc
     2761 gcagctctcg cgcgtcaggc ggcgcatgcc ctcgccttcg gcgccgagca cccacgcaat
     2821 tgatcgagga agatcagcct catagagcgt cttgtcagcg cgctcatcgg cgccgacgac
     2881 ccagatgttg tgctccttga cctcgccaag agtgcgcgcg aggttcgtaa ccataagata
     2941 cggcgtgttt tccgccgcgc cgcttgccac ttttgacact accggcgtga tgcctgcagc
     3001 gcggtcgcgc ggcgcgatta ctgcatgcgc acccgcggca ttcgcaactc gaaggca
//
LOCUS       DASUNT010000139         3606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1247301, whole genome shotgun sequence.
ACCESSION   DASUNT010000139 DASUNT010000000
VERSION     DASUNT010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247301"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1797
                     /locus_tag="VFX94_02750"
     CDS             <1..1797
                     /locus_tag="VFX94_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5767128.1"
                     /translation="LRESRETFAAQMSLLLNGGSVGSETLPATSAQTRPVLEALDVQW
                     KKNERNASLVLAEQANLVNLARAVRTINERNPALQELADEIAALSVQTGGSARQNAHA
                     AQLMMLTQRMAKNANTMLAEAVVDPEVSFLLGKDTNSFRDILRGLLQGSEALRIQRVA
                     DPELRGKLGEMEAAFKEYQAAVSGILGNQQRLVNAKRASFELFKDSEALLGAAETLNA
                     AYEAELGARRINFIAIGVVSFLAFVVLLLIGKTYLDDTRRRADENERQNRENQAAIRR
                     LLDEISNLANGDLTVRAQVTEHMTGAIADSINYTIDELRRLVAGINSASQQVTSATQE
                     AETVTGKLLQAAGKQASEIQGAGQSVAQMAQSMVEVSKKANDSSKVAESSLHAAQKGA
                     EAVQNAIRGMNDIRVQIQETSKRIKRLGESSQEIGEIVQLISDITEQTNVLALNAAIQ
                     AASAGEAGRGFTVVAEEVQRLAERSAEATKHISAIVKSIQRDTLEAVEAMERSTQGVV
                     QGTKTADQAEQALREIGAVSTQLAQLIDSISQATQQQATSASRVAANMRVILGITQLT
                     TDGTKQTATSAARLTALADGLKSSVAGFKLAA"
     gene            1794..>3606
                     /locus_tag="VFX94_02755"
     CDS             1794..>3606
                     /locus_tag="VFX94_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX5767129.1"
                     /translation="MMADTTVLSWINAEVERALTLVQESIAKFSAAPENEVVLKPCPE
                     HLHQVSGALRMVGLAGATRVCEAIENTFNGMGGARPTPAAIGVIDRAVVALKNFIGEL
                     ARGHADVPLRLFPVYRDITGLLGKTAVSEKDLFFPDLNLSAPAHASPRTVPQEERTAF
                     LQAERARFQRGLLAWLRGAPVGLRDMRQTVDALHQAATDLPEPRALWWAAGALVDSLK
                     SDAGDDPEWLASAKVVCNRIDFHIRDLGAGAAKNGNALLREALYALARREAATPRIKE
                     VKQLYQLETLFPRPEKSVPVLELDAERLEASLFDMHSRLEALKRSWEQYGAGERDQAR
                     VFRERVADFKLRTRDLGNAHLIKLLDAIALAAAKLPDPYSPDRQAMVIEMASAFLLVE
                     HVIDHFSNPSDDLELQIVIMGSWLLDAANGKSNGEPPPGLRPDLTERIGALQLRAQVA
                     KEILANLQHVEQVIDAFARDPGKRPTLPGLQPYLRQMHGALSVLGFPGAAQAVSICEG
                     LVKAQASADAAAEDVDWIAEALSSVGFFLEPCRHGREPGEEAIALFFRRYEKRNQPRA
                     PDSTLRIEFPSTSPAATAAPPPSPARAGVDAELLQVFL"
BASE COUNT          653 a         1203 c         1229 g          521 t
ORIGIN      
        1 ctgcgcgaga gccgcgagac cttcgcggcg cagatgagcc tgctcctgaa cggcgggtcg
       61 gtggggagcg aaacgctccc cgcgacctca gcccaaacgc gcccggtgct tgaggcgctg
      121 gacgtgcaat ggaagaagaa cgaacgcaac gcctcgctgg tgctcgccga gcaggcgaac
      181 ctcgtcaacc tcgcacgggc ggtgcgcacg atcaacgagc gcaatccggc gctgcaggag
      241 ctcgccgacg agatcgccgc gctcagcgtg cagaccggcg gctcggcgcg ccagaacgcg
      301 cacgccgcgc agctcatgat gctcactcag cgcatggcga agaacgcgaa caccatgctc
      361 gccgaggcgg tggtcgatcc cgaggtgtcg ttccttctcg gcaaggacac caatagcttc
      421 cgcgacattc tgcgcggcct gctccagggc agcgaagcct tgcgcatcca gcgcgtcgcc
      481 gatcccgagc tgcgcggcaa gctgggcgag atggaagcgg cgttcaagga ataccaggcg
      541 gcggtgagcg gcatcctcgg caaccagcag cggctggtga acgccaagcg cgcaagcttc
      601 gagctgttca aggacagcga ggcgctgctc ggcgctgcgg aaaccctgaa cgccgcctac
      661 gaagccgagc tcggcgcacg gcgtatcaac ttcatcgcca tcggtgtcgt atcgttcctg
      721 gcgttcgtcg ttctgctgct catcggcaag acgtacctgg acgacactcg ccgccgggcc
      781 gacgaaaacg agcgccagaa ccgcgagaac caggcggcca ttcggcgcct gctcgacgag
      841 atcagcaatc tggccaacgg cgacctcacg gtacgcgccc aggtgaccga gcacatgacc
      901 ggcgccatcg ccgactccat caattacacg atcgacgagc tgcgccgcct ggtcgccggc
      961 atcaacagcg cctcccagca agtgacgagc gccacgcagg aagccgagac ggtgaccggc
     1021 aagctgctgc aggcggcggg caagcaggcc tcggagatcc agggcgccgg ccagtcggtc
     1081 gcgcagatgg cgcagtcgat ggtcgaggta tcgaagaagg cgaatgactc gtcgaaggtc
     1141 gcggagagct cgctgcacgc ggcgcagaag ggcgccgagg cggtgcagaa cgcgattcgc
     1201 ggcatgaacg acatccgcgt gcagatccag gagacttcga agcggatcaa gcggctcggc
     1261 gaaagctcgc aggagatcgg cgagatcgtc cagctgatct cggacattac cgagcagacc
     1321 aacgtgctcg cgctgaacgc ggcgatccag gcggcttcgg cgggcgaggc cgggcgcggc
     1381 tttaccgttg tggcagagga ggtgcagcga cttgccgagc gctccgccga ggcgaccaag
     1441 cacatcagcg cgatcgtcaa gagtattcag cgcgacaccc tggaagcggt cgaggcgatg
     1501 gagcgcagca cccagggcgt ggtgcagggg acgaagaccg ccgaccaggc ggagcaggcg
     1561 ctgcgcgaga tcggcgcggt gtcgacgcag cttgcgcagt tgatcgattc gatctcgcag
     1621 gcgacgcagc agcaggcgac atcggccagc agggtggcag ccaacatgag ggtgatcctg
     1681 ggcattaccc agctcaccac cgacggcacc aagcagacgg cgacatccgc cgccaggctc
     1741 acggcgctcg ctgacggcct gaagagctcg gtggccggat tcaagctcgc ggcatgatgg
     1801 cggataccac cgtcctgtct tggatcaacg cggaggttga gcgggcgctg acgctcgttc
     1861 aggaatcgat cgcgaaattc tccgccgcgc ccgagaacga ggtggtgctc aagccgtgcc
     1921 ccgagcatct gcatcaggtg agcggggcgc tgcgcatggt cggcctcgcg ggagcgacgc
     1981 gcgtatgcga ggcgatcgag aacaccttca acggcatggg cggggcgcgg ccgacgccgg
     2041 cggcgatcgg agtgatcgac cgcgcggtgg tcgcgctcaa gaatttcatc ggcgagctcg
     2101 cccgggggca tgccgacgtg ccgctgcgcc tgtttccggt ctatcgcgac atcaccgggc
     2161 tgctcggtaa gacggcggtc tccgagaagg atctcttctt ccccgacctc aacctctcgg
     2221 cgcccgcgca cgcgagcccg cggacggtgc cgcaggaaga gcgcacagcg ttcctgcagg
     2281 ccgagcgtgc tcgtttccag cgcggactgc ttgcctggct gcgcggcgcg ccggtcggat
     2341 taagggacat gcgccagacg gtcgacgccc tgcaccaggc cgcaaccgac ctgcctgagc
     2401 cgcgggcgct gtggtgggcg gcaggcgcgc tcgtcgattc gctcaagagc gatgccggag
     2461 acgatccgga gtggctcgct tccgccaagg tcgtgtgcaa ccgcatcgat ttccacatcc
     2521 gcgatttggg ggccggagcg gcgaagaacg gcaatgcgct gctgcgcgaa gcgctgtatg
     2581 cgctcgcccg acgcgaggcg gcgaccccgc gcatcaagga ggtcaagcag ctttaccagc
     2641 tcgagacctt gtttccgcgc ccggagaaga gcgtcccggt gctcgagctc gatgccgagc
     2701 gcctggaggc atcgctcttc gacatgcatt cgcgactcga ggcgctcaag cgctcgtggg
     2761 agcagtacgg cgccggcgag cgcgaccagg cgcgcgtgtt ccgcgagcgc gtcgccgact
     2821 tcaagctgag gacccgggat ctcggcaacg cgcacctgat caagctgctc gatgcgatcg
     2881 ccctggctgc ggcgaagctg cccgacccct attcccccga caggcaggcg atggtcatcg
     2941 agatggcctc ggctttcctg ctcgtcgagc acgtcatcga ccatttcagc aacccgtcgg
     3001 acgacctcga gctccagatc gtcatcatgg gaagctggct gctcgatgcc gccaacggca
     3061 agtcgaacgg cgagccgccg ccgggcctgc gtcccgacct caccgagcgc atcggcgcgc
     3121 tgcagctgcg cgcacaggtc gccaaggaaa tcctggcgaa cctccagcac gtcgagcagg
     3181 tgattgacgc gtttgcccgc gatcccggga agcgtccgac gcttcccggc ctgcagccct
     3241 atctgcggca gatgcacgga gcgctgtccg tgctcggctt tccgggcgcg gcgcaagccg
     3301 tgtccatatg cgagggcctg gtcaaggcgc aggcgagcgc cgacgcagcc gccgaagacg
     3361 tcgactggat cgccgaagcg ctaagcagcg ttggattctt cctcgagccg tgccgccacg
     3421 gccgcgaacc cggcgaggaa gcgattgcgc tgtttttccg gcgctacgag aagcgcaatc
     3481 agccgcgggc accggacagc accttgagga tcgagttccc ttccacctcg cctgccgcta
     3541 cggctgctcc accgccgtcg cccgcgcgcg ccggggtcga tgccgagctc cttcaggtct
     3601 tcctgg
//
LOCUS       DASUNT010000140         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_545149, whole genome shotgun sequence.
ACCESSION   DASUNT010000140 DASUNT010000000
VERSION     DASUNT010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545149"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VFX94_02760"
     CDS             <1..1095
                     /locus_tag="VFX94_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX5767130.1"
                     /translation="GQGALLIANPKALDNPFYLAFPAWALYPMVALATAATIIASQAT
                     ITGAYSLTRQAIQLGFLPRVNIIQTSARQYGQIYIPAINWVLLILVLGAVFGFGSSSE
                     LASAYGVAVTGTMTATTILPFFVIRYGWGYPLWLCIFATGFFFLIDVTFFSAALLKIF
                     EGGWFPLVLGISMFTIMTTWRRGREVMFARLNEASVPLQPFLKSLFTDPPQRVPGTAV
                     FLTATPDATPHALLHNLNHNKVLHERVVFLTVEIRDVPWVSFEERVTCERLGHGCWRV
                     RVRYGFMNRPDVMRALEVCGALGLEFDFMQTSFFLSRQQIVPAPGGILPMARWRERMF
                     AAMARNAGNITDYFNIPTNRVIELGTKVEV"
     gene            complement(1178..2158)
                     /locus_tag="VFX94_02765"
     CDS             complement(1178..2158)
                     /locus_tag="VFX94_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HEX5767131.1"
                     /translation="MLINCVAYQEGRKLAELKLEEIHTYVARPDCFVWVALADPTNEE
                     LAAMQEEFKLHELAIEDAGHRHESVARPKVEEYGDTLFVVMNTVELLPTDEIKLGEMA
                     VFVGRNYVLSVRRRAERGFREVRANAERQPELLRHGPGFVLYALMDALVDRYFPVLEA
                     VDMELELVEERLFTPGASPRENIESLYYVKQQLTLLKHAAGPLLENAGKLFGGRVPAA
                     CSGLGEYFRDVYDHLIRINQSIDAARETVGTAIQVALAMVATRQGEITRMLAAYAALV
                     AVPTMIAGIYGMNFEHMPELEWTFGYPMTLGVIAAIDAFLFYRFRKAGWL"
BASE COUNT          357 a          775 c          715 g          422 t
ORIGIN      
        1 gggcaggggg cgctgctgat cgcgaatccc aaggcgctcg acaatccctt ctatctcgcg
       61 tttccggcct gggcgctcta cccgatggtc gccctcgcga ccgcggccac catcatcgct
      121 tcgcaggcga cgatcacggg cgcgtattcg ctcacgcgcc aggcgatcca gctcggcttc
      181 ctgcctcggg taaacatcat ccagacgtcc gcgcgccagt acggccagat ctacattccg
      241 gcgatcaact gggtgcttct catcctcgtg ctcggcgcgg tgttcggctt cggctcctcg
      301 agcgagcttg cctcggccta cggcgtcgcg gtcacaggca cgatgaccgc caccaccatc
      361 ctccctttct tcgtcatccg ctacggctgg ggctatccgc tgtggctgtg catcttcgcg
      421 accggctttt tcttcctcat cgacgtcact ttcttctctg cggcgctgct caagatattc
      481 gagggcggct ggtttccgct cgtgcttggc atctcgatgt tcacgatcat gacgacctgg
      541 cgccgcggcc gcgaggtgat gttcgcgcgg ctgaacgagg cttcggtgcc gctgcagcca
      601 ttcctcaagt cgctgttcac ggacccgcca cagcgcgtcc ccggcaccgc cgtgttcctc
      661 accgccacgc ccgacgccac gccgcacgcg ctgctccaca acctgaacca caacaaggtg
      721 ctgcacgagc gcgtggtgtt cctcaccgtc gagatccgcg acgtgccctg ggtgtcgttc
      781 gaggagcgcg tgacctgcga gcgcctcggg cacggctgct ggcgcgtgcg cgtgcgctac
      841 ggcttcatga accgccccga cgtcatgcgc gcgctcgaag tctgcggcgc gctcggcctg
      901 gagttcgact tcatgcagac ttcgttcttc ctctcgcgcc agcagatcgt gccggcgccg
      961 ggcggcattc tccccatggc gcgctggcgc gagcgcatgt tcgccgccat ggcgcgcaac
     1021 gccggcaaca tcaccgacta cttcaacatc ccgaccaacc gcgtgatcga gctcggaacc
     1081 aaggtcgagg tctaattcat tccacgaggc ggtagccgac cccggtctcg gtgagaaggt
     1141 gcttcgaaca gggtgagcgc atccatcgca ggtcatgcta gagccagccc gccttcctga
     1201 agcggtagaa caggaaagca tcgatggcgg cgatgacgcc cagcgtcatc gggtaaccga
     1261 aggtccattc gagctcgggc atgtgctcga agttcatgcc gtagatgccg gcgatcatcg
     1321 tcggcacggc caccagagcg gcgtaggcgg caagcatgcg cgtgatctcg ccttggcggg
     1381 tggccaccat ggcgagggcc acttgaatgg cagtgccgac cgtctcgcgc gcggcgtcga
     1441 tcgactggtt gatgcggatg agatggtcgt agacgtcgcg gaaatactcg ccgaggccgc
     1501 tgcaggccgc cggcacccgg ccgccgaaga gcttgcccgc gttctccagc agcgggccgg
     1561 cggcgtgctt gagcagcgtg agctgttgct tgacgtaata gagggactca atgttctcgc
     1621 gcggcgaggc gccgggggtg aacaggcgct cctccaccag ctcgagctcc atgtcgaccg
     1681 cctcgagcac cgggaaatac cggtccacca gggcgtccat cagcgcgtac agcacgaagc
     1741 ccgggccatg gcgcagaagc tccggctggc gctcggcgtt cgcgcgcacc tcccggaagc
     1801 cgcgctcggc ccgcctgcgc accgacagca cgtagttgcg gccgacgaac accgccattt
     1861 ccccgagctt gatctcgtcg gtcgggagga gctccaccgt gttcatgact acgaagaggg
     1921 tgtcgccgta ctcctcgacc ttggggcggg ctacggattc gtggcggtgg ccggcatctt
     1981 cgatcgcgag ctcgtgcagc ttgaactcct cctgcatggc ggcgagctct tcattggtcg
     2041 ggtcggccag cgctacccag acgaagcagt ccgggcgcgc cacataggtg tgaatctcct
     2101 ccagcttcag ctctgcgagc ttgcggccct cctggtacgc gacgcagttg atgagcatgc
     2161 gcggatgcta acgcacgcgg acatccgggg acgggacatt cggggacgca gtcctatctg
     2221 gttacggctc cgtcccggaa ttacttggcg ccgaggccca tgcgcttgc
//
LOCUS       DASUNT010000141         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_18937, whole genome shotgun sequence.
ACCESSION   DASUNT010000141 DASUNT010000000
VERSION     DASUNT010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18937"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VFX94_02770"
     CDS             <1..1029
                     /locus_tag="VFX94_02770"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX5767132.1"
                     /translation="ASGMQALDSAINNILAGRSSLVLAGGVDALSRAPLLYGEKMVLW
                     FSQLASTKSLPQRMALLARLPLGSLLAPVIGIMKGLTDPMVGLLMGQTAENVAYRFGI
                     TRAEMDAFSVRSHERVIRAQDEGRLAPSGGEVEPLYDTKGNVYTLDDGVRRDASLHNL
                     AKLKPFFDRKFGNVTPGNSSQITDGAAWLILASEDVVRNNNLQPLGRILDSEWAGLDP
                     AHMGLGPVHAATPILKRNHLELDAIAYWEINEAFAAQVLGCLAAWQDGQYCREQLGLN
                     KALGSLNEERLNVDGGAIALGHPVGASGARIVLHLLKTLKRNNARRGMAAICIGGGLG
                     GAMLVEAV"
     gene            1029..>2178
                     /locus_tag="VFX94_02775"
     CDS             1029..>2178
                     /locus_tag="VFX94_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5767133.1"
                     /translation="MERVDAKVFAKHWRWEKDGEGLVWLMFDKQGESTNTFSREALNE
                     LSLIVGEIAAARPRGLIIRSAKEGFIAGVDIEELTRLDSGAEALELTRLGWDVFQKVR
                     DLPFPTTALINGFCMGGGTELALACRYRVALDDPKVRIALPEVMLGIMPVWHGVQWLP
                     KLVGPGPAFDLLLTGKAIDAKRAKRIGLVDQAVPLRILENTARMVTLEDRGRRQPPLL
                     QRLMLLMRGFVVSQARRQVAKKARREHYPAPYAILELWQKYDGDPFINPSDPSCSITA
                     LFDHPTTENLIRIFFLQERLKGLGKSTDFRPQQVHVVGAGVMGGDIAAICAMRGLRVT
                     LQDTAPERIAPAIKRAAELFRRRLRDARRERDALDRLMPDVTGEGARHA"
BASE COUNT          375 a          731 c          732 g          340 t
ORIGIN      
        1 gcctccggca tgcaggcgct cgattcggcc atcaacaaca tcctcgccgg ccgctcgagc
       61 ctcgttctcg ccggcggcgt cgatgcgctc tcgcgtgcac cgcttctcta cggcgagaag
      121 atggtgctgt ggttttcgca gctcgcctcg accaaaagcc ttccgcaaag aatggcgctg
      181 ctcgcgcggc tcccgctcgg gtccctgctt gctccggtga tcggaatcat gaaggggctg
      241 accgacccga tggtcggcct gctgatggga cagaccgcgg agaacgtcgc gtaccgcttc
      301 ggcatcacgc gcgccgaaat ggatgcattt tccgtccgca gccatgaacg agtcatacgc
      361 gcccaggacg aagggcgcct cgcgcccagc ggcggcgaag tcgagccgct gtacgacacc
      421 aagggcaacg tctacacgct cgacgacggc gtgcggcgcg acgcctcgct gcacaacctc
      481 gccaagctca agcccttctt cgaccgcaag ttcggcaacg tgacgcccgg caacagctcg
      541 cagatcaccg acggcgcggc gtggctgatc ctcgcctcgg aagacgtggt gaggaacaac
      601 aatctccagc ccctcggccg catcctcgat tccgagtggg ccggcctcga cccggcgcac
      661 atgggcctcg gcccggtgca cgcggccacg ccgatcctga agcgaaacca tctcgagctg
      721 gacgccatcg cctactggga gatcaacgag gcgttcgccg cccaggtgct cggctgcctg
      781 gcggcatggc aggatgggca atactgcagg gaacagctcg ggctcaacaa ggctttgggt
      841 tctctcaatg aagaaagatt gaacgtcgat ggcggggcaa tcgcgctcgg gcatccggtc
      901 ggcgcttcgg gcgcgcgcat cgtcctgcat ctgctgaaaa cattgaagag aaacaacgcg
      961 aggcgcggaa tggccgcgat ctgcatcggc ggcggcctgg gcggcgccat gctagtggag
     1021 gctgtgtgat ggaacgagtc gacgccaagg tcttcgcgaa gcactggcgc tgggagaagg
     1081 acggcgaagg gctcgtctgg ctcatgttcg acaagcaggg cgaatcgacc aatacctttt
     1141 cgcgggaggc gctcaacgag ctttcgctca tcgtcgggga gatcgccgcc gcgcggccga
     1201 gggggctcat catccgatcg gcgaaagagg gctttatcgc cggggtcgac atcgaggagc
     1261 tcacgcgcct ggactcgggc gccgaagcgc tcgagctgac gcggctcggc tgggacgttt
     1321 tccagaaggt gcgcgacctg cccttcccca ccacggcgct gatcaacggc ttttgcatgg
     1381 gcggcggcac cgagcttgcg cttgcctgcc gctaccgcgt cgcgctcgac gacccgaagg
     1441 tgcgcatcgc gctgcccgaa gtgatgctcg gcatcatgcc ggtgtggcac ggcgtgcaat
     1501 ggctgcccaa gctcgtcggc ccgggacccg cgttcgatct gctgctcacc ggcaaggcga
     1561 tcgacgcgaa gcgcgcgaaa cgcatcggcc tcgtcgacca ggcggtgccg ctgcgtatcc
     1621 tggagaacac cgcgcgcatg gtgacgctcg aagaccgcgg ccgcaggcaa ccgccgctgc
     1681 tgcagcggct gatgctcctc atgcgcggat tcgtggtctc gcaggcgcgc cggcaggtgg
     1741 cgaagaaggc gcggcgcgag cactatccgg cgccctacgc catcctcgag ctgtggcaga
     1801 agtacgacgg cgatccgttc atcaatccgt cggacccgtc gtgctcgatc acggcgctct
     1861 tcgaccaccc gacgaccgag aacctgatcc gcattttctt cctgcaggag cggctgaagg
     1921 gcctcgggaa gagcacggac ttcaggccgc agcaggtgca cgtcgtcggc gccggggtga
     1981 tgggcggcga cattgcggca atctgcgcca tgcgcgggct gcgcgtcacg ctgcaggaca
     2041 ccgcgcccga gcgcatcgcg ccggcgataa agcgcgccgc cgagcttttc aggcgccggc
     2101 tgcgcgatgc gcggcgcgag cgcgacgcgc tcgaccgcct gatgcccgac gtgaccggcg
     2161 aaggcgcgcg ccacgccg
//
LOCUS       DASUNT010000142         2786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_633194, whole genome shotgun sequence.
ACCESSION   DASUNT010000142 DASUNT010000000
VERSION     DASUNT010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633194"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VFX94_02780"
     CDS             complement(<1..350)
                     /locus_tag="VFX94_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX5767134.1"
                     /translation="MRSRSHGAAVAREYLQALEQQRRLSPATLRNYGRALGLLLKQTD
                     SLETLEPADVRRFIAMLHAKGASPRTLALTLSAWRGYFRWLVRHRGLRANPVLGIRAP
                     RAARPLPKALSVEAA"
     gene            complement(320..964)
                     /locus_tag="VFX94_02785"
     CDS             complement(320..964)
                     /locus_tag="VFX94_02785"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEX5767135.1"
                     /translation="MNSEDIARYLRAHPQFFDKHPELLESISVPHPHGGRAIPLAERQ
                     TVALREKLKLLEGRLAELLQLAGENDAISEKVHRLSVALCGARDFPALAAALYFHLRE
                     DFAVPHVALRVWGKSVPADFDEAQAVDEAQRLHAGAMAAPQCGPAAGNPFVPWFSEAA
                     EHVRSVALVPLGQTTVFGLLALGSEDAKRFYPEMGTLYLRRIGELCAAAVTARL"
     gene            complement(987..1781)
                     /gene="dapF"
                     /locus_tag="VFX94_02790"
     CDS             complement(987..1781)
                     /gene="dapF"
                     /locus_tag="VFX94_02790"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX5767136.1"
                     /translation="MRLGFTKMHGAGNDFVVLDFTRQPFDLDSSQLRRLADRHFGVGC
                     DQVLVVERPREPQADFRYRIFNADGGEVEQCGNGARCFVKFVHQRGLTAKAEIRVETL
                     GGTITPRLEPDGEVSVDMGRPSRPTLERLEVQGGQVELTILSMGNPHAVQIVPDVAAA
                     PVTRQGPALEVHPRFPHRVNAGYMQVLDRHAIALRVWERGAGETLACGTGACAAAVAG
                     ISRGLLDSPVKVRARGGTLTVSWTGGDNAVFMKGPAVSVFEGEIEL"
     gene            complement(1778..>2786)
                     /locus_tag="VFX94_02795"
     CDS             complement(1778..>2786)
                     /locus_tag="VFX94_02795"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX5767137.1"
                     /translation="GRFVLGPEGEALERELATFLGASHAVGVNSGTDALHLPLVAAGI
                     GPGDEVVVPGFTFFATGEAVSYTGATPVFADVDEGTFNLDPASLTRNITRKTRAVIAV
                     HLFGQCAALDQIAEICKARNLILVEDCAQCIGADYDGRRAGSWGDFGAFSFYPTKNLA
                     AAGDAGMISAKSEDHAKTLRMLRHHGSRQTYLHERVGWNSRLDELQAAVLRVKLKHLA
                     RFNEARRAVARRYREKLAGAGVLLPVEHGRGTHVYHQFTIRAERRDAIREALAKDGIA
                     SSVFYPLALHQQPAYAGIAKGVSLPVAERVAKSVLSLPIHPLLDEASIERIAAGVRAA
                     A"
BASE COUNT          426 a          951 c          966 g          443 t
ORIGIN      
        1 gcggcttcca ccgacagcgc cttgggcagc ggtcgcgccg ccctaggcgc gcggatgccg
       61 agcaccggat tggcgcgcag gccgcggtgg cgcacgagcc agcgaaaata gccccgccag
      121 gccgaaagcg tgagcgcaag cgtgcgcggc gaggctcctt tggcatgcaa catcgcgatg
      181 aagcggcgca cgtcggccgg ctcgagcgtt tcgagggaat ccgtttgctt caggagcagg
      241 ccgagagcgc ggccgtagtt cctcagcgtc gcgggcgaga ggcgccgctg ttgctcgagc
      301 gcctggaggt actcgcgcgc tacagccgcg ccgtgactgc ggctgcgcat agttcgccga
      361 tgcggcgcag gtacagcgtg cccatctcgg ggtagaagcg cttcgcgtcc tcgctgccga
      421 gcgcgagcag gccgaacacc gtggtctggc cgagcggcac gagcgcgacc gagcgcacgt
      481 gctcggccgc ctcgctgaac caggggacga acgggttgcc ggcggcgggg ccgcattgcg
      541 gcgcggccat cgcgcctgca tgcaggcgct gcgcctcgtc cacggcttga gcctcgtcga
      601 aatccgcggg gacgctcttg ccccagacgc gcagcgcgac gtgcggcacg gcgaaatcct
      661 cgcgcaggtg gaagtagagc gcggctgcga gcgccggaaa gtcgcgcgcg ccgcaaaggg
      721 caaccgacag gcggtgcacc ttctcgctga tcgcgtcgtt ttccccggcg agctgcagca
      781 gctcggcgag ccggccttcg agcagcttca gcttctcgcg cagcgccacc gtctggcgct
      841 cggcgagcgg gattgcgcgt ccgccgtggg gatgcggcac gctgatcgac tcgagaagct
      901 ccgggtgctt gtcgaagaac tgcggatggg cgcgcaggta gcgcgcgatg tcttcggagt
      961 tcatctcgct gtgatgggct tcgttcttat agttctatct cgccttcgaa tacgctgact
     1021 gccggtccct tcatgaacac ggcattgtcg ccgcccgtcc aagagacggt caatgtgccg
     1081 ccgcgggcgc gcaccttcac cggcgaatcg agcagcccgc gcgaaatccc ggccaccgcg
     1141 gcggcgcacg cgcccgttcc gcacgcgagc gtttcgcccg cgccgcgctc ccagacgcgc
     1201 agtgcgatgg catggcgatc gagcacctgc atgtagcccg cgttcacccg gtgcggaaag
     1261 cgcgggtgca cctcgagcgc agggccttgc cgcgtcacgg gcgcggctgc cacgtcgggc
     1321 acgatctgca cggcgtgcgg gttgcccatg gagaggatgg tcagttccac ctgcccgccc
     1381 tgcacctcca gccgctcgag cgtcggccgc gacggccggc ccatgtcgac gctcacttcg
     1441 ccatcgggct caaggcgcgg cgtgatagtg ccgccgagcg tctcgacgcg gatttctgcc
     1501 ttggccgtga gcccgcgctg gtgaacgaat ttcacgaagc agcgcgcgcc gttgccgcac
     1561 tgctctacct cgccgccgtc ggcgttgaag atgcggtagc gaaagtcggc ttgcggctcg
     1621 cgcggccgct cgacgaccag cacctgatcg cagccgacgc cgaaatggcg gtccgcgagt
     1681 cttctgagct gggacgaatc gaggtcgaac ggctgccggg tgaaatcaag aaccacgaaa
     1741 tcgttccccg cgccgtgcat cttcgtgaag ccgagcctca cgccgcggct cgcacgccgg
     1801 ccgcgatgcg ttcgatcgaa gcctcgtcga gcagcggatg gatcggaagc gagagcacgc
     1861 tcttcgcgac tctctccgcc acgggcaacg aaacgccctt cgcgattccc gcgtacgcgg
     1921 gctgctgatg gagcgcaagc gggtagaaca ccgagcttgc tatgccgtcc tttgcgagcg
     1981 cttcgcgaat ggcgtcgcgg cgctcggcgc ggatggtgaa ctggtggtag acgtgcgtgc
     2041 cgcgcccgtg ctcgacgggc aggagcaccc ccgccccggc gagcttctcc cgatagcggc
     2101 gcgccactgc gcgccgcgcc tcgttgaagc gcgcgagatg cttcagcttg acgcgcagca
     2161 ccgcagcctg cagctcgtcg aggcggctgt tccagcccac gcgctcgtgc aggtaagtct
     2221 ggcggctacc gtggtggcgc agcatgcgga gcgttttcgc atggtcttcg ctcttcgcgc
     2281 tgatcatccc tgcatcgccc gccgccgcga ggttcttggt cgggtagaac gagaaggcgc
     2341 cgaagtcgcc ccagcttccg gcccgcctgc cgtcgtagtc ggcgccgatg cactgcgcgc
     2401 agtcttcgac caggatgaga tttctcgcct tgcagatttc ggcaatctga tcgagagccg
     2461 cgcactggcc aaaaagatgg accgcgatca cggccctggt ctttctcgtg atgttcctgg
     2521 tgagtgaagc agggtcgaga ttgaaggtgc cctcgtccac gtcggcgaac accggcgtcg
     2581 cgcccgtgta ggacaccgct tcgccggtgg cgaaaaaggt gaagcccgga accaccacct
     2641 cgtcgccggg accgatgccg gccgcgacga gcggcaggtg cagcgcgtcg gtgcccgagt
     2701 tgacgcccac ggcgtggctc gcgccgagga atgtcgcgag ctcgcgctcg agggcctcgc
     2761 cctcggggcc gagcacgaag cggccc
//
LOCUS       DASUNT010000143         2681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_370768, whole genome shotgun sequence.
ACCESSION   DASUNT010000143 DASUNT010000000
VERSION     DASUNT010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370768"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VFX94_02800"
     CDS             <1..819
                     /locus_tag="VFX94_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HEX5767138.1"
                     /translation="IHVNDPDFREHLFRENFNMHTSTSPQYGLIASLDVARKQAVMEG
                     YKLLLRTLDLAHEVKKLINSTGVFRVLELDDLLPEAVRHDGIKLDPTKVTIDISGCGY
                     TVEDLQKALFERFNIQVEKSTFNTLTLLLTIGTTRSKVSRLYDALMRIAREGHTPRRL
                     VQTPEVPAFTKLRFLPRDAYYCGGELVPMFDESERVNRRLAGRVAADQIVPYPPGIPV
                     LVPGQLITRDIVEYLGALLHSHKRMEMHGVVHEGYVPCIRVLKPAEEAALHRLS"
     gene            885..2075
                     /locus_tag="VFX94_02805"
     CDS             885..2075
                     /locus_tag="VFX94_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5767139.1"
                     /translation="MSHVMNTYARQPVAFVRGEGVWLWDETGKKYLDALAGIAVNTLG
                     YAHPKLKKALAERLASGVLHTSNLWRIPEQEKAADRVAEITGLEEVFFCNSGLEANEA
                     AIKVARKYGHDRGIAEPAIIVMEKAFHGRSLATLSATGSRKVQAGFEPLVSGFVRVPL
                     NDLEAVRQVAERNKNVVAVFVEPIQGEGGINVSRLEYLRGLKELCERKEWLFMSDEVQ
                     CGLGRTGKWFVYQHAGFLPDVVPLAKGLGSGVPVGACVVGGRAKGVFKPGNHGSTFGG
                     NPLAMTAVVATIDTMKEEGLLANALRVGEVIKSGLASIAGTVEVRGMGLMIGVELAAP
                     CGELVRQALEAGLVINVTADNVVRLLPPLVMSEAEGRMVVERLAPLVRAFVERSSQAT
                     AAAR"
     gene            2086..>2681
                     /locus_tag="VFX94_02810"
     CDS             2086..>2681
                     /locus_tag="VFX94_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX5767140.1"
                     /translation="MAVRHFLRLSDFSLTELEHVFARTRWIKDRFKRYERYWPLEDRT
                     LAMIFEKPSTRTRMSFEAGMHQLGGAAIYLYTRDSQLGRGEPVEDAAQVISRMCDVIM
                     VRTFEQTIIERFAGASRVPVINGLTNDYHPCQILADVFTYIEHRGPIRGRTVAWIGDS
                     NNVCNTWLQAAEIFDFKLNISTPPGYEVEQERMNRRSEA"
BASE COUNT          466 a          898 c          888 g          429 t
ORIGIN      
        1 atccatgtga acgacccgga tttccgcgag catctgttcc gcgagaactt caacatgcac
       61 acctcgacga gccctcagta cgggctgatc gcgagcctcg acgtcgcgcg caagcaggcg
      121 gtgatggagg gctacaagct gctgttgcgc acgctcgacc tcgcgcacga ggtgaagaag
      181 ctcatcaact ccacgggcgt ctttcgcgtg ctcgagctcg atgacctcct gcccgaggcg
      241 gtgcgccacg acggcatcaa gctcgacccg accaaggtga cgatcgacat ctcgggctgc
      301 ggctacaccg tcgaagacct gcagaaagcc ctcttcgagc gcttcaacat ccaggtcgag
      361 aagtccacct tcaacacatt gaccttgtta ttgaccattg gaacgacccg gtcgaaggtc
      421 tcgcggctct acgacgcgct tatgcgcatc gcgcgcgagg ggcacacgcc gcggcggctc
      481 gtgcagacgc cggaggttcc cgcttttacg aagctccgat ttctcccgcg tgacgcctac
      541 tactgcggcg gggagctcgt cccgatgttc gacgagagcg agcgcgtgaa ccgccgcctc
      601 gccgggcgcg tcgccgcaga ccagatcgtc ccctacccgc ccggcatccc ggtgctcgta
      661 ccggggcagc tcatcacccg cgacatcgtg gagtacctcg gcgcgctatt gcactcgcac
      721 aagcgcatgg agatgcacgg ggtggtgcac gagggctacg tgccctgcat ccgcgtgctg
      781 aagcccgcgg aagaagccgc gctacaccgc ctgtcttagg cgggcgtggt accatttctg
      841 tgaacggcgg ccattgccgc cgttttgcat ttcgggagag cgcgatgagc catgtgatga
      901 acacctacgc acgccagccg gttgcgttcg tgcgcggcga aggcgtctgg ctctgggacg
      961 aaacgggcaa gaaatacctc gacgccctcg ccggcatcgc ggtgaacacg ctgggttacg
     1021 cgcatccgaa gctcaaaaag gcgctggccg aaagactcgc ctcaggagtg ctgcacacgt
     1081 ccaacctgtg gcgcatcccc gagcaggaaa aggcggccga ccgcgtcgcc gagatcaccg
     1141 ggctcgagga ggtgttcttc tgcaactcgg gcctggaggc gaacgaggcc gcgatcaagg
     1201 tggcgcggaa atacggccac gaccgcggca tcgccgagcc tgcgatcatc gtcatggaga
     1261 aggccttcca cggccgctcg ctcgccacgc tgtcggccac cggcagccgc aaggtgcagg
     1321 cggggttcga gccgctcgtc tccggcttcg tgcgggtgcc gctcaacgac ctcgaggccg
     1381 tgcgccaggt cgccgagcgc aacaagaacg tcgtcgcggt cttcgtcgag cccatccagg
     1441 gcgaaggcgg catcaacgtg tcgcgcctgg aatacctgcg cgggctgaag gagctctgcg
     1501 agcgcaagga gtggctcttc atgtccgacg aggtgcagtg cggactgggc cgcaccggca
     1561 agtggttcgt ctaccagcac gccgggttcc ttcccgacgt ggtgccgctc gcgaagggcc
     1621 tcggctccgg cgtgccggtg ggcgcgtgcg tcgtcggcgg cagggcgaaa ggggtgttca
     1681 agcccggcaa ccacggctcg acgttcggcg gcaacccgct cgcgatgacg gcggttgtcg
     1741 ccacgatcga cacgatgaag gaagaaggcc tgctcgccaa tgcactgcgc gtcggcgaag
     1801 tgatcaagtc gggattggcc tccatcgccg gcacggtcga agtgcgcggc atggggctga
     1861 tgatcggggt cgagctcgcg gcgccgtgcg gcgagctcgt gcgccaggcc ctcgaagccg
     1921 ggctcgtcat caacgtcacc gcggacaacg tcgtgcggct tctgccgccg ctcgtgatga
     1981 gcgaggcgga gggccggatg gtggtcgagc gccttgcgcc gctcgtgcgc gcgttcgtgg
     2041 agcgcagctc ccaggccacc gccgcggcgc gctgagcggc gcgccatggc cgtgcggcac
     2101 ttcctgcggc tcagcgattt ctcgctcacg gagctcgagc acgtgttcgc gcgcacgcgc
     2161 tggatcaagg acaggttcaa gcgctacgag cgctactggc cgctcgagga ccgcacgctc
     2221 gcgatgatct tcgagaagcc ctcgacgcgc acgcgcatgt cgttcgaggc cggcatgcac
     2281 cagctcggcg gcgccgcgat ctatctgtac acgcgcgact cgcagctcgg ccggggcgag
     2341 ccggtggagg acgcggcgca ggtgatctcc cgcatgtgcg acgtgatcat ggtgcgcacc
     2401 ttcgagcaga cgatcatcga gcgcttcgcc ggcgcctcgc gcgtgccggt gatcaacggc
     2461 ctcaccaacg actaccatcc ctgccagatc ctggccgacg tcttcactta catcgagcac
     2521 cgcggcccga tccgcggccg caccgtggcg tggatcggcg actcgaacaa cgtgtgcaac
     2581 acctggctgc aggcggccga gatcttcgac ttcaagctca acatctccac cccgcccggc
     2641 tacgaggtcg agcaggaaag aatgaatcgc cgcagcgaag c
//
LOCUS       DASUNT010000144         5633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1072096, whole genome shotgun sequence.
ACCESSION   DASUNT010000144 DASUNT010000000
VERSION     DASUNT010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072096"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VFX94_02815"
     CDS             complement(<1..1163)
                     /locus_tag="VFX94_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEX5767141.1"
                     /translation="MPKPASRILVINDEHLVLREFVKGLNAAARTLDNPLGISFIGVA
                     TAKEALQVIESDGNLQAVLVDDTLYSLPTRNSNGKKDELRPRRLQMSALELVQRITRF
                     RPEIDVYIVIAQEKEDDVVDALFAESVDGYFYREERDYRGMYRILNAQIQERARTPFY
                     DQLKNYVWMAKDQWHTPGHSSGESLRGSPWVNDFYEFMGEHVFDADLSVSVPMLDSLM
                     EPKGVIGEAQAMAAKAFGARRTFFATNGTSTANKVIFQTLLAPGEKLLLDRNCHKSVH
                     HGVVLSGAHPVYLDSALNRKYGLYGPVPKSLLLSSIRKHSDAQALILTSCTYDGLRYD
                     LAPVIDAAHKRGIKVIIDEAWYGFARFHPHFRPTALEAGADYVTQSTHKVLSA"
     gene            complement(1244..1507)
                     /gene="rpsT"
                     /locus_tag="VFX94_02820"
     CDS             complement(1244..1507)
                     /gene="rpsT"
                     /locus_tag="VFX94_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX5767142.1"
                     /translation="MANIKSARKRAKQAVARRDHNMSLRTAVRSAIKNVKKALAAGDR
                     DAAARTLRESQRVIDRIVAKGVLHRNAGDRHKSRLAHALKAMK"
     gene            1523..3061
                     /gene="murJ"
                     /locus_tag="VFX94_02825"
     CDS             1523..3061
                     /gene="murJ"
                     /locus_tag="VFX94_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX5767143.1"
                     /translation="MNLLRILATVSSLTMVSRVLGYVRDFFIARVFGAGLATDAFFVA
                     FKLPNLLRRLFAEGAFSQAFVPLLAEHKHRDEHEAKRLIDAVSTLLFLVLALTAALGV
                     AAAPVIVYVTAPGFALDPEKPFELTVALLRVTFPYILFISLVSLSAGILNTWNRFSVP
                     AITPALLNVSFIVGAAFFAQHFDPPVMVLAWAVLLGGILQLAFQVPFLLKLRLVPRWR
                     IDLSHPGLRRVMLLMLPAAFGVSVSQVSLVLNQIFASFLKSGSVSWLYYADRLMELPA
                     GVLGVAVGTILLPSLSKYHAAANAEEYSRLLDWGLRITVLLAVPAAAALALLALPLIA
                     MLFQYGRFSAEDAWMTRQALVAYSVGLVGMILVKILAPGFYARQNVATPVKIGVLTLV
                     ATQLMNLAFIGALKHAGLALAIGLGACLNALLLYLMLRRQRIYVPQPGWPVFALKVLA
                     SVGFMAIVLFSTMGEASWWLGAPWQHKAPAVLGLVLLGAAAYGACLLAFGFRPRDFSR
                     RAAE"
     gene            3061..3915
                     /locus_tag="VFX94_02830"
     CDS             3061..3915
                     /locus_tag="VFX94_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5767144.1"
                     /translation="MGAALEPFARLIAGEDARIDLARACLLIAQDAYPQLDVEHYMNE
                     IERMATRLRARLPRDCGAEQQVVALNQFLYEDLGYWGNTEDYYDPRNSYLNEVIERKT
                     GMPITLSILYMELGRRIGLPLEGVSFPGHFLVRLPLRGGMLVLDPFSGGAPQSEAELR
                     SRVKRVIPDGVADDLPAAELPLDQFLERATNRQILTRVLRNLKAIYRKTDKPERLLDV
                     LDRMLIVTPDASAELRDRGFVYQRLECYRAALKDLSSYAEREPDAPDLDEVRSRLMEL
                     SALCARLN"
     gene            complement(3825..4403)
                     /locus_tag="VFX94_02835"
     CDS             complement(3825..4403)
                     /locus_tag="VFX94_02835"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HEX5767145.1"
                     /translation="MKTLEDQMSFYAAYHQDVRNKATHFVGVPAIMLSLFIPLAWIRL
                     LEVNGVVVTAAMLFAAIVLVYYFVLDVPLGLAMLLVTSVLLWIAHAIAGLGAVAGWTA
                     FAILFVGGWILQLVGHVFEGRKPALADNLFQIFVAPIFLAAEVFFALGYKPVLHQRVK
                     QRAQLSRAQSADSSISLERTSSRSGASGSRSA"
     gene            4438..5340
                     /locus_tag="VFX94_02840"
     CDS             4438..5340
                     /locus_tag="VFX94_02840"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX5767146.1"
                     /translation="MVVTHGSLQRARGRCALTIGNFDGVHRGHRALIDRVTAKAREAQ
                     LTSCILTFEPHPREFFAPDAAPARITRLRDKLELMAQAGVEHVHVARFDARFAAMTAE
                     RFIEDVLVRRLAVQWLLVGRDFRFGARRAGDFATLRNRGFELEAMPDVLFEGKRVSSS
                     AVRAALKAGELEQAERLLGHPYTISGRVAHGAKLGRGLGFPTANIVLRRAPPLGGIFV
                     VEVDNYGPGVASIGRRPTINPVAIPLLEVHLFDHDADLYGEHLRVRFLKKLRDEEKYD
                     GLPALQAAIARDARQAREFFLSHG"
     gene            5333..>5633
                     /locus_tag="VFX94_02845"
     CDS             5333..>5633
                     /locus_tag="VFX94_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5767147.1"
                     /translation="MADYKDTLNLPETPFPMRGDLARREPQWVKEWQDKGVYRRLRAL
                     AKGRPRFVLHDGPPYANGDIHIGTAVNKILKDIVVKSKTLAGFDAPYVPGWDCHGM"
BASE COUNT          879 a         1888 c         1840 g         1026 t
ORIGIN      
        1 aacgcggaca gcaccttgtg cgtcgactgc gtcacgtagt ccgcgcccgc ctcgagcgcg
       61 gtcggccgga agtgcgggtg aaagcgcgca aagccgtacc acgcttcgtc gatgatcacc
      121 ttgatgccgc gcttgtgcgc cgcgtcgatg accggcgcga ggtcgtagcg caggccgtcg
      181 taggtgcagg aggtgaggat cagcgcctgc gcatccgaat gttttcttat ggagctcagc
      241 aacaagctct tcggcaccgg gccgtagagc ccgtacttcc ggttgagcgc cgaatcgagg
      301 tacaccgggt gcgcgcccga cagcaccacg ccatggtgca cgctcttgtg gcagttgcgg
      361 tcgaggagga gcttctcgcc cggcgcgagc agcgtctgga agatgacctt gttcgccgtg
      421 gaggtgccgt tggtggcgaa aaaggtgcgc cgcgcgccga acgccttggc cgccatcgcc
      481 tgcgcctcgc cgatcacccc cttcggctcc atcagcgaat cgagcatggg caccgatacc
      541 gacagatcgg cgtcgaacac gtgctcgccc atgaactcgt agaagtcgtt cacccacggg
      601 ctgccgcgca gtgactcccc cgaggagtgc ccgggggtat gccactggtc ctttgccatc
      661 cagacgtagt tcttgagctg gtcgtagaac ggcgtgcgtg cgcgctcctg gatctgcgcg
      721 ttgaggatgc ggtacatgcc gcggtaatcg cgctcctcgc ggtagaagta gccgtcgacc
      781 gattcggcga aaagcgcgtc caccacgtcg tcttcctttt cctgcgcgat gacgatgtag
      841 acgtcgatct cgggacggaa gcgcgtgatg cgctgcacga gctcgagcgc cgacatctgc
      901 aggcggcgcg gccgcagctc gtccttcttc ccgttgctgt tcctggtggg aagcgaatac
      961 agggtatcgt cgacgagcac ggcctggagg ttgccgtctg actcgatgac ctggagcgct
     1021 tccttcgcgg tggcgacgcc gatgaagctg atgccgagcg ggttgtcgag cgtgcgcgcc
     1081 gccgcgttca agcccttgac gaactcgcgc agcacgagat gctcgtcgtt gatgacgagg
     1141 atgcggctcg cgggcttggg catcaggccc ggttacttat aatcgcgcat gcccggagcc
     1201 ttcgccggag atgcagcgtg tcgaaatacg gcccagccct tgactacttc atcgctttca
     1261 gcgcgtgcgc caggcggctc ttgtgacgat cgccggcgtt gcggtgcagg acgcctttcg
     1321 ccacgatccg gtcgatcacg cgctgggact cgcgcagggt cctcgccgcc gcgtccctgt
     1381 cgccggcggc gagcgctttc ttcacgttct tgatcgcgct gcgcaccgcg gtgcgcaggc
     1441 tcatgttgtg gtcgcgtcgc gccaccgcct gcttcgcgcg cttgcgcgcg gacttgatgt
     1501 ttgccattga aaaaattcta gcatgaacct gctgcgtatt ctggccacgg tcagcagcct
     1561 cacgatggtg agccgcgtcc tcggctacgt gcgcgacttc ttcatcgctc gggtgttcgg
     1621 ggcggggctc gcgaccgacg cattcttcgt ggcattcaag ctgccgaacc tgctgcggcg
     1681 gctgttcgcc gagggggcct tctcgcaggc tttcgtgccg ctccttgccg agcacaagca
     1741 ccgggacgag cacgaagcga aacgcctgat cgacgcggta tccacgctgc tcttcctcgt
     1801 cctcgcgctc accgccgcgc tcggcgtggc ggccgcgccg gtcatcgtct acgtcaccgc
     1861 gcccggattc gccctcgacc cggagaagcc tttcgagctg accgtagccc tgcttcgcgt
     1921 caccttcccc tacatcctgt tcatttccct tgtatcgctg tcggcgggaa ttcttaacac
     1981 ctggaaccgc ttttccgtcc ccgcgatcac cccggcgctg ctcaatgtgt cgttcatcgt
     2041 gggcgctgcc ttcttcgcgc agcacttcga tccgccggtc atggtccttg cctgggcggt
     2101 cctcctcggc ggcatcctgc agctcgcctt tcaggtgccg ttcctcctca agctgcgcct
     2161 cgtgccgcgc tggcgcatcg atctctcgca tcccggtctg aggcgcgtaa tgctgctcat
     2221 gctgcccgcc gcattcggcg tttcggtaag ccaggtgtcg ctcgtcctca accagatttt
     2281 cgcctcgttc ctcaagagcg gaagcgtgtc gtggctttac tatgccgacc ggctgatgga
     2341 gcttcccgcc ggcgtgctcg gggtcgcggt cggcacgatc ctgctgccga gcctttccaa
     2401 gtaccacgcc gcggcgaatg ccgaggaata cagccggctg ctcgactggg ggctcaggat
     2461 caccgtgctg ctcgcggtgc cggcagcggc cgcgctcgcg ctactcgcct tgccgctcat
     2521 cgcgatgctc ttccagtacg gacgcttcag cgccgaggac gcgtggatga cgcgccaggc
     2581 gctcgtcgcc tacagcgtcg ggctggtcgg catgatcctc gtcaagatcc tcgcgccggg
     2641 cttctatgca agacagaacg tggccactcc ggtcaagata ggcgtcctta cgctcgtggc
     2701 gacacagctc atgaacttgg ccttcatcgg tgcgctcaag cacgccgggc tcgcgctcgc
     2761 catcggcctg ggcgcctgcc tcaacgcgct gctcctctac ctcatgctca ggaggcaaag
     2821 gatctacgtg ccgcagccgg gctggccggt cttcgcgctg aaggtgctag cttccgtcgg
     2881 gttcatggcg atcgtgcttt tttcgacgat gggcgaggcc tcgtggtggc tcggcgcgcc
     2941 ctggcagcac aaggcgcccg cggtgctcgg actcgtgctg ctgggcgccg cggcctacgg
     3001 cgcctgcctg ctcgctttcg gcttccgccc gcgcgacttc tcgcgccgcg cggccgagtg
     3061 atgggcgccg cgcttgagcc gtttgcccgc ctcatcgccg gcgaggacgc gcgcatcgac
     3121 ctcgcgcggg cgtgcctgct catcgcgcag gacgcatacc cgcagctcga cgtcgagcac
     3181 tacatgaatg agatcgagcg catggcgacc cgcctgcgcg cccggctgcc gcgagactgc
     3241 ggcgcagagc agcaggtcgt ggcgctcaac cagttcctct acgaagacct cggctactgg
     3301 gggaacaccg aggattacta cgatccgcgc aacagctatc tgaacgaggt gatcgagcgc
     3361 aagaccggga tgccgatcac gttgtcgatc ctgtatatgg agctcgggcg gcgcatcggc
     3421 ctgccgctcg agggagtgtc tttcccgggg cactttctcg ttcgcctgcc gctgcgcggc
     3481 gggatgctgg tgctcgatcc gttcagcggc ggcgctccgc agtccgaggc cgagcttcgc
     3541 agccgggtga agcgcgtgat tccggacggc gtagccgacg acctgccggc cgcggagctg
     3601 ccgctcgacc agttcctcga acgggcgacg aaccgccaga ttctcacccg cgtgctgcgc
     3661 aacctgaagg ccatctaccg caagaccgac aagcccgagc gcctgctcga cgtgctcgac
     3721 cgcatgctca tcgtcacgcc cgacgcgagc gcggagctgc gagaccgcgg cttcgtgtac
     3781 cagcgcctgg agtgctaccg cgcggcgctc aaggacctga gcagctatgc cgagcgcgag
     3841 cccgacgcgc cggatctcga cgaggtgcgc tccaggctga tggagctatc cgcgctctgc
     3901 gcgcggctca actgagctct ttgcttcacc ctttggtgta gaacgggctt gtaccctagg
     3961 gcaaagaaca cctcggccgc caggaagatc ggcgcgacga agatctggaa caggttgtcc
     4021 gcgagcgccg gtttgcggcc ctcgaagacg tggccgacga gctgcaggat ccagccgccg
     4081 acgaagagga tggcaaacgc cgtccagccc gcgacggcgc cgaggccggc gatcgcgtgg
     4141 gcaatccaga ggaggaccga agtgacgagc agcatcgcaa ggccgagggg aacatcgagc
     4201 acgaagtagt agacgagcac gatcgcagcg aacagcatcg ccgcggtaac gacaacaccg
     4261 ttgacttcga gcaaccggat ccacgcgagc gggatgaaaa gcgacagcat gatcgcgggc
     4321 acaccgacga agtgcgtggc cttgttccgc acgtcctgat ggtacgcggc gtagaagctc
     4381 atctggtctt cgagggtttt cataatctga ctataattca ggcctttaac cgcgtccatg
     4441 gtggtcacgc acggctcttt gcagcgcgcc cgcggccggt gtgcgctgac gatcggaaac
     4501 ttcgacggcg tgcaccgcgg gcatcgcgcg ctcatcgacc gcgtcaccgc gaaggcgcgc
     4561 gaagcgcaac tcacgtcgtg catcctgact ttcgagccgc atccgcgcga atttttcgcc
     4621 ccggatgcgg cgccggcccg catcacgcgg ctgcgcgaca agctggagct gatggcgcag
     4681 gccggcgtgg agcatgtgca cgtcgcgcgc ttcgacgctc gcttcgcggc catgacggcc
     4741 gagcgcttca tcgaagacgt gctggtgcgc aggctcgccg tgcagtggct gctggtgggg
     4801 cgggatttcc gcttcggcgc ccggcgagcc ggggactttg cgacgttgag aaaccgcggc
     4861 ttcgagctcg aggcgatgcc cgacgtgctc ttcgagggca agcgcgtctc gagctccgcg
     4921 gtgcgtgcgg cgctcaaggc gggcgagctc gagcaggccg agcgcctgct cggccatcct
     4981 tacacgatca gcgggcgcgt cgcgcacggc gcgaagcttg gccgcggcct cggtttcccg
     5041 accgcgaaca tcgtgctgcg gcgcgcgccg ccgctcggcg gcatcttcgt ggtcgaagtc
     5101 gacaactacg gccccggggt cgcgagcatc ggcaggcgcc cgacgatcaa cccggtcgcc
     5161 attccgctgc tggaagtgca tcttttcgac cacgatgccg atctctacgg ggagcatctg
     5221 cgcgtgcgct tcctgaagaa gctgcgcgat gaagaaaagt acgacggcct tcctgcgctg
     5281 caggccgcga tcgcgcgcga cgcgaggcag gcgcgcgaat tctttctgag ccatggctga
     5341 ctacaaagac acgctgaacc ttcccgagac gccgtttccg atgcgcggcg accttgccag
     5401 gcgcgagccg cagtgggtga aggagtggca ggacaagggc gtctaccgcc ggctgcgcgc
     5461 cctcgccaag ggccggccgc gcttcgtgct gcacgacggg ccgccgtacg cgaacggcga
     5521 catccacatc ggcaccgcgg tgaacaagat cctgaaggac atcgtcgtga agtcgaagac
     5581 cctggccggc ttcgacgcgc cctacgtccc cggctgggac tgccacggca tgc
//
LOCUS       DASUNT010000145         3893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_545808, whole genome shotgun sequence.
ACCESSION   DASUNT010000145 DASUNT010000000
VERSION     DASUNT010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545808"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1517)
                     /locus_tag="VFX94_02850"
     CDS             complement(120..1517)
                     /locus_tag="VFX94_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767148.1"
                     /translation="MKNLEDVLRAAGNPVDMLRNSQIGAYVYPVVPSEFSNWRDEQLA
                     WQKSAVLFDQSHHMVEQYVEGPDALKLFSSVGANSFANFPVDRAKQFAPTNYDGYVIG
                     DGILFHLAQESLVFVGRAPAASWIQFHAETGGYDVVTHKDDRSPGNTRGHAIVRRNYR
                     FQIQGPNAWKVIEKLNGGPVPDIRFFNMGRIRIAGRKVRALRHGMAGAPGLEIWGPYA
                     EREEVRAAIVTAGREFGLKEVGARAYATNTLESGWIPSPLPAVYTGEKMKTYREWLPA
                     TSYEATGSIGGSFDSNSIEDYYVTPYEMGYGSFIKFDHDFIGRQALEKMTGKPHRKKV
                     TFAWNADDVAAVWRSMLEPGDPYKYIDLPLSNFTSATYDKVIGRAGKVVGVNMFSGYS
                     YNERSMLSLGIVDPDIELGTELKLVWGEPGSGSKKPTVERHRQTEIRVIVSPVPYANQ
                     ARTSYAEGWRTKATV"
     gene            complement(1539..2696)
                     /gene="metK"
                     /locus_tag="VFX94_02855"
     CDS             complement(1539..2696)
                     /gene="metK"
                     /locus_tag="VFX94_02855"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX5767149.1"
                     /translation="MSDFLFTSESVSEGHPDKVADQISDAVLDAILKQDKTSRVAAET
                     LVKDNLVVLAGEITTSARVSYDEVARSTIRRIGYTEPTLGFDAATCTVMLAYGQQSPD
                     IARGVNESTNMDLEQGAGDQGLMFGYACDETPSLMPLPIFLSHRLVERQAELRRNGRL
                     PWLRPDAKSQVTIRYVNAKPDSIDTVVLSTQHAPGIEHKKLAEAVVEEVIKPVLPKSM
                     VKGKINYLVNPTGAFEIGGPKGDCGVTGRKIIVDTYGGSAPHGGGAFSGKDPSKVDRS
                     AAYAARYVAKNIVAAGLASRCLVQVSYAIGVARPTSIMVTTHGTGKLSDDALSALVTK
                     HFDLRPKGIIQMLDLLRPIYEKTAAYGHFGREEPEFTWEATDKALALKQAA"
     gene            2797..3645
                     /locus_tag="VFX94_02860"
     CDS             2797..3645
                     /locus_tag="VFX94_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5767150.1"
                     /translation="MFRPLLRLYAHLPLWLLHGLGTVLGWAIYGMSPTYRSNLRQNLQ
                     RAGYGEARVRRAAIAHAGRLVAETPAIWLRPQERVAALVREVRGRAAIDAARAAGKAL
                     LFLTPHMGCFEITAQWAAQRMAITVLYRPPKLAWLDPLMRAGRSRGGMRLAPADARGV
                     REVLAALKRREAVGFLPDQVPGEGEGEWAQFFGELAYTGTFAPRLAQRADLACLLAYA
                     ERLPRGTGYRLQFRPLPSRVPGESAVRHLNRALEDLVRECPGQYLWGYNRYKTPAGAK
                     PKPAAA"
     gene            3642..>3893
                     /locus_tag="VFX94_02865"
     CDS             3642..>3893
                     /locus_tag="VFX94_02865"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HEX5767151.1"
                     /translation="MSRIVFWFMWLVHFLPLGLQARIGSFTGSILFWMIAERRKVTRI
                     NLAKCFPRMDPIARENLARAHFRALSRAFIEQGILWWSPR"
BASE COUNT          562 a         1308 c         1293 g          730 t
ORIGIN      
        1 cggctggcgc gctgcccagg cgcactttct cgcgcagctc gtgcagctcg cgcggccgca
       61 ggaggcttcc gacgtgatcg gctctgaagg ggggagctga aagtccggcc atcaggtcct
      121 tataccgtcg ccttggttct ccagccctct gcgtacgagg tgcgcgcctg attggcgtag
      181 ggcacgggac tgacgatgac tcggatctcg gtctggcggt ggcgctccac cgtgggcttc
      241 ttggagccgc tgccgggctc cccccagacg agcttgagct cggtgccaag ctcgatgtcc
      301 ggatcgacga tgccgagcga gagcatcgag cgctcgttgt agctgtagcc gctgaacatg
      361 ttgacgccca ccaccttgcc ggccctgccg atcaccttgt cgtaggtggc cgaggtgaag
      421 ttggaaagcg gcaggtcgat atacttgtac ggatcgcccg gctcgagcat cgagcgccac
      481 accgcggcga cgtcgtcggc gttccaggcg aaggtgactt tcttgcggtg cggcttcccg
      541 gtcatttttt ccagcgcctg ccggccgatg aagtcgtggt cgaacttgat gaatgagccg
      601 tatcccatct cgtagggcgt gacgtaatag tcctcgatgc tgttggaatc gaagctaccg
      661 ccgatcgagc cggtcgcctc gtagctcgtg gccggcagcc actcgcggta cgtcttcatc
      721 ttctcgccgg tgtacaccgc cggtagcggc gaaggtatcc agcccgattc gagcgtgttg
      781 gtggcatagg cacgagcgcc cacttccttg aggccgaact ccctgcctgc ggttacgatc
      841 gccgcgcgca cttcctcacg ctcggcgtag ggcccccaga tctccaggcc cggcgcgccc
      901 gccatgccat ggcgcagcgc ccgcaccttg cggccggcaa tcctgatgcg gcccatgttg
      961 aagaacctga tatccggcac cgggccgccg ttcagcttct cgatcacttt ccaggcgttc
     1021 gggccctgga tctggaaccg gtagttgcgc cgcacgatgg cgtggcccct ggtgttgccg
     1081 ggcgagcgat cgtccttgtg cgtgaccacg tcgtagccgc cggtctcggc atggaactgg
     1141 atccagctcg ccgccggcgc gcgaccgacg aagaccaggc tctcctgcgc caggtggaac
     1201 aggattccgt cgccgatgac gtagccgtcg tagttggtcg gggcgaactg cttcgcgcgg
     1261 tcgaccggga aattggcgaa gctgttcgcg ccgacgctcg agaagagctt cagcgcatcc
     1321 gggccctcga cgtactgctc caccatgtgg tgcgactggt cgaagagcac cgcgcttttc
     1381 tgccaggcga gctgctcgtc gcgccagttg gagaattcgc tcggcaccac gggatacacg
     1441 taggcgccga tctgcgagtt gcgcagcata tcgaccggat tgccggccgc cctcaggacg
     1501 tcctcgaggt tcttcatagc ctcctcagta cagtggagct aggctgcctg cttcagcgcg
     1561 agagccttgt cggtcgcttc ccacgtgaac tcgggctcct cgcgcccgaa gtggccgtat
     1621 gccgcggtct tctcgtagat cgggcgcagc aggtcgagca tctggatgat gcccttcggc
     1681 ctgagatcga aatgcttggt gacgagcgcc gaaagcgcgt cgtcggacag cttcccggtg
     1741 ccgtgcgtcg tgaccatgat gcttgtcggg cgcgcgacgc cgatggcgta cgagacctgc
     1801 accaggcacc tggacgcgag cccggcggcg acgatgttct tcgccacgta gcgcgcggcg
     1861 taggcggccg agcggtcgac cttggaagga tccttgccgg aaaacgcgcc gccgccgtgc
     1921 ggcgccgagc cgccgtaggt gtcgacgatg atcttgcgcc cggtcacgcc gcagtcgccc
     1981 ttcggcccgc cgatctcgaa tgcgccggtg gggttcacga ggtaattgat cttccccttc
     2041 accatgctct tcggcagcac cggcttgatg acttcctcca ccaccgcctc cgcaagcttc
     2101 ttgtgctcga tgccgggagc gtgctgcgta gagagcacca cggtgtcaat cgagtcgggc
     2161 ttggcgttca cgtagcgtat cgtgacctgc gatttcgcgt ctggccggag ccagggcagc
     2221 cggccgttgc gccgcagctc ggcctggcgc tcgacgaggc gatgcgacag gaagatgggc
     2281 agcggcatca ggctgggagt ctcgtcgcag gcgtagccga acatcaggcc ctggtcgccc
     2341 gcgccctgct cgaggtccat gttcgtgctt tcgttcaccc cgcgcgcgat gtcgggggac
     2401 tgctggccgt aggcgagcat gacggtgcac gtggcagcgt cgaagccgag cgttggctcg
     2461 gtgtagccga tgcgccggat ggtgctacgg gccacctcgt cgtaactcac gcgcgcgctg
     2521 gtggtgatct cgccggcgag gaccaccagg ttgtccttga ccagcgtctc ggccgccacg
     2581 cgcgaggtct tgtcctgctt tagaatcgcg tcgagcacgg cgtcggagat ctgatcggcg
     2641 actttgtcgg gatggccttc ggacaccgat tcggaggtaa acagaaaatc gctcatcgcc
     2701 tgcgcttcgt ctgctgaatg ggaaggccgc aaggatacca aaacttgtcc cgctgcccct
     2761 agccccggtc ctatccttcg tccgcttttc ctcgtcattt ttcgtccgct actccggctt
     2821 tacgcccatc tgccgctttg gctgctgcac gggctgggaa ccgtcctggg ctgggcgatc
     2881 tatgggatgt cgcccacgta ccgcagcaac ctgcgccaga atctgcagcg cgccggctac
     2941 ggcgaggcgc gggtgcgccg cgcggccatc gcccacgcag gccgtcttgt cgccgagacg
     3001 cccgcgatct ggctgcgccc ccaggaacgc gttgccgcgc tggtgcgcga ggtgcgcggc
     3061 cgcgccgcga tagacgccgc gcgcgcagcc gggaaggctt tgctcttcct cacgccgcac
     3121 atgggttgct tcgagatcac ggcgcagtgg gcggcgcagc gcatggccat cacggtgctc
     3181 taccgcccgc cgaagctggc ctggctcgat ccgctgatgc gcgcggggcg cagccgcggc
     3241 ggcatgcgcc tcgcgccggc ggacgcgcgc ggcgtgcgcg aggtgctcgc cgcgctgaag
     3301 cgccgcgagg ccgtcggctt cctgcccgac caggtgccgg gcgaagggga gggtgaatgg
     3361 gcgcaattct tcggcgagct cgcttacacc ggaaccttcg cgccgcgtct cgcgcagcgc
     3421 gcggacctcg cctgcctgct tgcctacgcc gagcgcctgc cgcgcgggac gggctaccgc
     3481 ctccagttca ggccgctgcc gtcccgggtt cccggcgaga gcgcggtgcg ccacctgaat
     3541 cgcgcgctcg aggacctggt gcgcgagtgt cccggccagt acctgtgggg ctacaaccgc
     3601 tacaagactc ccgcgggcgc gaagccgaaa cccgccgccg catgagccgc atcgtcttct
     3661 ggttcatgtg gctcgtgcac ttcctaccgc tcgggctcca ggcacgcatc ggctctttca
     3721 ccggctccat ccttttctgg atgatcgcgg agcggcgcaa ggtgacgcgc atcaacctgg
     3781 cgaaatgctt cccgcgcatg gacccgatcg cgcgcgagaa tctcgcgcgc gcgcatttcc
     3841 gggcgctttc ccgcgctttc atcgagcagg ggatcctgtg gtggtcgccg cgc
//
LOCUS       DASUNT010000146         2861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1160156, whole genome shotgun sequence.
ACCESSION   DASUNT010000146 DASUNT010000000
VERSION     DASUNT010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160156"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1669)
                     /locus_tag="VFX94_02870"
     CDS             complement(<1..1669)
                     /locus_tag="VFX94_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5767152.1"
                     /translation="MKAKIFGNAPTRSRKLQILLAILAVLLAVDGFLVWQDARQATFG
                     TLYIATVGKIRMLSQRLAKAAQQASQGNIEAFKQLRESRDAFAAQMNLLLNGGQVAGD
                     SLPATSSQTRPVLDALEAQWKKNERNASLVIAEQVNLVNLGRAVRSINERNPALQELA
                     DEIAALSVQTGGSTRQNAYAAQLMMLTQRMAKNANAMLAEAVIDPEVSFLLGKDTNGF
                     RDILQGLLQGSEALRIQRVSDAELRGKLAEMQAAFKDYQVAVASVLGNQQRLVSAKRA
                     SFQLFQDSEALLGAAETLNTAYEAELGERRTNFIAMAVVSLLALIVLALIGKTFFDDT
                     RRRAEESERQNRENQAAIQRLLDEISNLANGDLTVRAQVTEHMTGAIADSINYTIDEL
                     RRLVAGINNASQQVTSATQEAESVTGKLLQAASKQAAEIQGAGRSVAQMAQSMVEVSK
                     KAGDSSKVAESSLHAAQKGAEAVQNAIRGMNDIRVQIQETSKRIKRLGESSQEIGNIV
                     ELINDIAEQTNILALNASIQASMAGEAGRGFAVVADEVQRLAERAANS"
     gene            complement(1682..2812)
                     /locus_tag="VFX94_02875"
     CDS             complement(1682..2812)
                     /locus_tag="VFX94_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5767153.1"
                     /translation="MLRTAAFALAFAALLPGAAHGDDGVYPDRIVLGQAAVFTGPAAQ
                     LGIQMRNGIKAYFDYVNAKGGVHGRRLDLVTEDDRYEQAVAPSASKKLIEEHRVFALL
                     GYVGTPTGVAHLPVVTQAKVPLVGMFTGAEVLRAPFNRYVFHVRASYYDETEKIVEQV
                     LSTGGQKISVFYQDDAYGEAGRKGTELALERRRMKIHSSGTVERNTVKVEQAAKTIHA
                     SQPDAIVMVSAYTSIAAFVREMQKLGSGATFYNVSFVGSKALADALGKEGVGVAISQV
                     VPFPWGTAVPVVKEYQQLAKKAGFTDYNFSALEGFLTAKVMVEALRRAGKAPTREGLV
                     DTLEKMNDVDLGGFYISYSPKNRAGSKFVDLTIISRDGKFLR"
BASE COUNT          398 a          959 c          955 g          549 t
ORIGIN      
        1 tcgagttcgc ggcgcgctcg gcgagccgct gcacctcgtc ggcgacgacg gcgaaaccgc
       61 ggccggcctc gcccgccatc gaggcctgta tcgacgcgtt gagcgcgaga atgttggtct
      121 gctcggcgat gtcgttgatc agctcgacga tgttgccgat ttcctgcgag ctctcgccca
      181 ggcgcttgat tcgcttcgag gtttcctgga tctggacccg gatgtcgttc atgccgcgga
      241 tcgcgttctg caccgcctcg gcgcccttct gcgccgcgtg cagcgagctc tccgccacct
      301 tggatgaatc cccagctttc ttcgagacct cgaccatcga ttgcgccatc tgcgcgaccg
      361 accggccggc gccctggatc tcggcggcct gcttgctcgc cgcctgcagg agcttcccgg
      421 tcacgctctc ggcttcctgc gtggcgctcg tcacctgctg cgacgcgttg ttgatgcctg
      481 ccaccaggcg gcgcagctcg tcgatcgtgt agttgatgga gtcggcgatg gcgccggtca
      541 tgtgctctgt gacctgcgcg cgtaccgtga ggtcgccgtt cgcgaggttg ctgatctcgt
      601 cgagcaggcg ctggatcgcc gcctggttct cgcggttctg gcgctcgctc tcctccgcgc
      661 gccggcgggt atcgtcgaag aacgtcttgc cgatcaaggc gagcacgatc aaggcgagca
      721 gcgacaccac ggccatggcg atgaagttcg tgcgccgctc gccgagctcc gcttcgtacg
      781 cggtgttcag cgtttccgcg gcgccgagca gcgcctcgct gtcctggaag agctggaagc
      841 tcgcgcgctt ggcgctcacc agacgctgct ggttgccgag cacgctcgcc accgcgacct
      901 ggtagtcctt gaacgccgct tgcatttcgg ccagcttgcc gcgcagctcg gcgtcggaca
      961 cgcgctggat gcgcagcgct tcgctgccct ggagcaggcc ctgcaggatg tcgcggaagc
     1021 cgttggtgtc tttgcctagc aggaacgaca cctccgggtc gatcaccgcc tcggccagca
     1081 tggcgttcgc gttcttcgcc atgcgctgcg tcagcatcat gagctgcgcg gcgtaggcgt
     1141 tctgccgcgt cgagccgccg gtctgcacgc tgagggccgc gatctcgtcg gcgagctcct
     1201 gcagcgcggg attgcgctcg ttgatggagc gcaccgcgcg cccgaggttg acgaggttca
     1261 cctgctcggc aatgacgagc gaggcgttgc gctcgttctt cttccactgc gcctccagcg
     1321 cgtcgagcac cggccgcgtc tgcgacgacg tcgccggcag actgtcgccg gcaacctggc
     1381 ctccgttcag gaggagattc atctgcgccg caaaggcgtc gcggctctcg cgcagctgct
     1441 tgaacgcctc gatgttgccc tgcgaggcct gctgcgcggc cttcgcgagg cgctgcgaga
     1501 gcatccggat cttgcccacg gtggcgatgt acagcgtgcc gaaggtcgcc tggcgcgcgt
     1561 cctgccacac caggaagccg tcgacggcga gcaggaccgc caggatcgcg agcaggatct
     1621 gcagcttgcg gctgcgggtg ggggcatttc caaagatctt ggctttcatg gggtcctcgt
     1681 cctagcgcag gaatttgccg tcgcgcgaga tgatcgtcag gtcgacgaac ttcgagccgg
     1741 cgcggttctt gggcgaatag ctgatgtaga agccgccgag gtcgacgtcg ttcatcttct
     1801 cgagcgtatc cacgaggcct tcgcgggtcg gagccttgcc cgcgcggcgc agggcctcca
     1861 ccatcacctt ggcggtgagg aagccctcga gcgcgctgaa gttgtaatcg gtgaagccgg
     1921 ctttcttcgc gagctgctgg tattccttca ccaccggcac cgcggtgccc caggggaacg
     1981 gcaccacctg cgagatcgcg acgccgacgc cctccttgcc gagcgcgtcg gcgagcgcct
     2041 tgctgccgac gaacgacacg ttgtagaacg tggcgccgct gccgagcttc tgcatctcgc
     2101 gcacgaaggc ggcgatggac gtgtaggcgc tcaccatgac gatcgcgtcg ggctgcgagg
     2161 cgtggatggt tttcgcggcc tgctccacct tgaccgtatt gcgctcgacg gtgccgctcg
     2221 aatggatctt catcctgcgc cgctcgagcg cgagctcggt gcccttgcgc ccggcttcgc
     2281 cgtaggcgtc gtcctggtag aacaccgaga tcttctgtcc gccggtggag agcacctgct
     2341 cgacgatctt ctcggtctcg tcgtagtagc tcgcgcgcac gtggaatacg tagcgattga
     2401 aaggcgcgcg cagcacctcg gcgcccgtga acatgccgac caacggcacc ttcgcctgcg
     2461 tcacgaccgg cagatgcgcg acgccggtcg gcgttccgac gtagcccagc aaagcgaaca
     2521 cgcggtgctc ctcgatcagc ttcttgctgg cgctgggggc gaccgcctgc tcgtagcggt
     2581 cgtcctccgt gacgaggtcg agcctgcgcc cgtgcacccc gcctttcgcg ttcacgtagt
     2641 cgaagtacgc cttgatgccg ttgcgcatct ggatgccgag ctgcgccgcc gggccggtga
     2701 agacggccgc ctggcccagc acgatgcggt cggggtagac gccgtcatcg ccgtgcgcgg
     2761 cccccgggag gagcgcggcg aaggcgagag cgaaagcagc ggtgcggagc atagaggtct
     2821 ccttctgttc ttcttagttg cctgcagcaa gaaatgcggc g
//
LOCUS       DASUNT010000147         3814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_809153, whole genome shotgun sequence.
ACCESSION   DASUNT010000147 DASUNT010000000
VERSION     DASUNT010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809153"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1231
                     /gene="prfB"
                     /locus_tag="VFX94_02880"
     CDS             join(127..207,209..1231)
                     /gene="prfB"
                     /locus_tag="VFX94_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417196.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX5767154.1"
                     /translation="MEAERINQLASSLSDLQARSAELRRYLDFDSKQERLAALNRELE
                     DPKLWENPQRAQELGKEKKSLEAVVGTLDRVGGGLSDAKELFEMAREESDDATLTSIF
                     ADIEKLAKDVAGLEFRRMFSNPLDPNNCFMDIQAGAGGTEAQDWAAMLERMYLKFCDR
                     RGFNAEVLEHTPGEVAGIKSATLKVRGDYAYGTLRTETGVHRLVRKSPFDANARRHTS
                     FASVFVYPEVDESIEVEVNPADLRVDTYRASGAGGQHVNRTDSAVRITHLPTNIVVQC
                     QNDRSQHRNRAEAMAMLKAKLYELELRKRQAEQQKLEDAKTDIGWGHQIRSYVLDQSR
                     IKDLRTGVEKGATQAVLDGELDDFIEASLKQGV"
     gene            1233..2684
                     /gene="lysS"
                     /locus_tag="VFX94_02885"
     CDS             1233..2684
                     /gene="lysS"
                     /locus_tag="VFX94_02885"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX5767155.1"
                     /translation="MEEENKLVDERREKLKALRSAGLTFPNDFRRSHLAGELAARFGD
                     KTKEELEKARVEAKVAGRMMLKRVMGKASFATLQDGSGRIQIYVVSELKNYEDFKHWD
                     LGDILGVEGHLFKTMKGELTIHARSIRLLVKALRPLPEKFHGLADHELRYRQRYVDLI
                     VNPQVKDVFGKRIEIIRTLRETLFADGYLEVETPMMQPIPGGAAARPFKTHHNALDMD
                     LYLRIAPELYLKRLVVGGMERVFEINRNFRNEGISTKHNPEFTMLEWYCAYEDWRNMM
                     DHTERLVRAAAGNAVVTYQGKRLDFSKPFARLSIPDAIRKEGVQGDLRDRKLLSEKLD
                     SLNIQYEKHQGWGALQLMLFETIAEKHLIEPTFVTDFPAEVSPLSRRRDRDPDVADRF
                     ELFIDAKEIANGFSELNDPEDQAARFLEQARRREAGDQEAMYYDADYVRALEYGMPPC
                     AGGGLGIDRLVMLLTDSPSIRDVILFPHMRPEG"
     gene            complement(2681..3580)
                     /gene="rssA"
                     /locus_tag="VFX94_02890"
     CDS             complement(2681..3580)
                     /gene="rssA"
                     /locus_tag="VFX94_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HEX5767156.1"
                     /translation="MRRKLRLGLALGSGSARGWAHIGVIRALEERGIKPDMVCGSSIG
                     ALVGAAYASGDLDRLEKWACSLSWATVVRLMDLTWRGGLIRGTRLFALFRALLEDREI
                     DELPLPFGAIATELASGRELWLRHGKVLDAVRASCAMPGLLTPVVREGAVLVDGGLVN
                     PVPVSMCRALGADLVVAVDLSWGKLGPYRRGKETELAPREVPGWLDRLRPLIASNWLK
                     SKPKPEETPDIPSLFNVFMTSLDIVEMRVARSRLAGEPADVLITPLLPDFATMDYHRA
                     REAIAEGRAAVDRMGPLLEQVLG"
BASE COUNT          742 a         1240 c         1257 g          575 t
ORIGIN      
        1 ggctgcaggg cgcgttcgtc tcgtcgagcg gcacggtgtc gcgcctcgag acgctcgagg
       61 cgcaggccct gttccagcgc gagctgcgct tctgggagag ccgcgtcgcc gtgcgataat
      121 ccggcgatgg aagccgagcg catcaaccag ttagcctcct ctctgtctga cctgcaggcg
      181 cggtcggcgg agctgcggag gtatctttga cttcgactcg aagcaggagc gcctggcggc
      241 gctgaaccgc gagctcgaag accccaagct gtgggagaac ccgcagcgcg cgcaggagct
      301 gggcaaggaa aagaagtcgc tggaggcggt ggtcggcacg ctcgaccgcg tcggcggcgg
      361 cctgtccgac gcgaaagagc tgttcgagat ggcgcgcgag gagtccgacg acgccacgct
      421 gaccagcata tttgccgaca tcgagaagct ggcgaaggac gtggcgggcc tcgagttccg
      481 ccgcatgttc tccaacccgc tcgacccgaa caactgcttc atggacatcc aggccggcgc
      541 cggcggcacc gaggcgcagg actgggcggc gatgctggag cgcatgtacc tcaagttctg
      601 tgaccggcgg gggttcaacg cagaggtgct ggagcacacc ccgggagaag ttgccggcat
      661 caagagcgcg acgcttaagg tgaggggcga ttacgcgtac ggcacgctgc gcaccgagac
      721 cggagtgcac cgtctggtgc gcaagagccc gttcgatgcc aatgcccgcc ggcacacctc
      781 gtttgccagc gtcttcgtct accccgaggt cgacgagtcg atcgaggtcg aggttaatcc
      841 ggcggatctg cgcgtagaca cttaccgcgc ttccggagcg ggcggccagc acgtgaaccg
      901 caccgacagc gcggtgcgca tcacacacct gccgacgaac atcgtggtgc agtgccagaa
      961 cgaccgctcg cagcaccgca accgcgccga ggcgatggcg atgctcaagg cgaagctcta
     1021 cgagctcgag ctgcgcaagc ggcaggccga acagcagaag ctcgaggatg cgaagaccga
     1081 catcggctgg gggcaccaga tccgcagcta cgtgctcgac cagtcgcgca tcaaggacct
     1141 gcgcaccggc gtggagaaag gcgcgaccca ggcggtgctc gacggcgagc tcgacgactt
     1201 catcgaagcg agcctcaagc aaggagtcta gtttggaaga agaaaacaag ctggtcgacg
     1261 agcggcgtga gaagctgaag gcgctgcgta gcgctgggct cacgttcccc aacgacttcc
     1321 ggcgctctca cctcgccggc gagctcgccg cacggttcgg tgataagacg aaggaggagc
     1381 tcgagaaagc gcgcgtcgag gcgaaggtcg cggggcgcat gatgctcaag cgcgtgatgg
     1441 gcaaagcgag ctttgccacc ctgcaggacg ggagtggccg gatccagatc tacgtagtga
     1501 gcgagctgaa aaactacgag gacttcaagc actgggacct gggcgacatc ctcggcgtgg
     1561 aaggccacct cttcaagacg atgaaaggcg agctgacgat ccatgcaagg agcatccggc
     1621 tgctggtgaa ggcgctgcgg ccgctacccg agaagttcca cggccttgcc gaccacgagc
     1681 tgcgctaccg acagcgctat gtagacctga ttgtcaaccc gcaggtgaag gatgttttcg
     1741 ggaaaaggat tgaaatcatt cgcacgctgc gcgagacgct gttcgcagat ggctatctcg
     1801 aggtcgagac gccgatgatg cagccgattc cgggcggcgc cgcggcgcgg ccattcaaga
     1861 cccaccacaa cgcgctcgac atggatcttt accttcgtat tgcgcccgag ctctacctca
     1921 agcgcctcgt ggtcggcggc atggagcgcg tgttcgagat caaccgcaat ttcaggaacg
     1981 agggcatctc gaccaagcac aaccccgaat tcacgatgct cgagtggtac tgcgcctacg
     2041 aggactggcg caacatgatg gaccacaccg agcggctggt gcgcgccgcg gccggcaacg
     2101 ccgtcgtgac gtaccagggc aagcgcctgg atttcagcaa gcccttcgcg cgcctctcca
     2161 ttcccgatgc gatccgcaag gaaggcgtgc agggcgactt gcgcgaccga aaacttcttt
     2221 cggaaaagct cgattcattg aacatccaat acgaaaagca tcagggctgg ggagcgctgc
     2281 agctcatgtt gttcgagacg attgccgaga agcacctgat cgagccgacc ttcgtcaccg
     2341 atttccccgc cgaggtgtcg ccgctctcgc gccggcgcga ccgcgacccc gacgtcgccg
     2401 accgcttcga gctgttcatc gacgcgaagg agatcgcgaa cggattctcc gagctgaacg
     2461 accccgaaga ccaggcggcg cgcttcctcg agcaggcgcg caggcgcgag gcaggggacc
     2521 aggaggcgat gtactacgac gccgactacg tgcgcgcgct cgaatacggc atgcctccgt
     2581 gcgccggcgg cggcctgggc atcgaccgcc tagtgatgct gctcaccgat agcccgagca
     2641 tccgggatgt gattctcttc ccgcacatgc ggcccgaggg ctagcccaat acctgctcga
     2701 gaagggggcc catgcggtcg accgccgcgc ggccctcggc gatcgcctcg cgcgcgcggt
     2761 ggtaatccat ggtggcgaag tccggcagca gcggcgtgat gagcacgtcg gccggctcgc
     2821 cggcgaggcg gctccgcgcc acgcgcatct cgacgatatc gagcgaagtc atgaacacgt
     2881 tgaagagcga cggaatgtcc ggtgtttcct ccggcttcgg cttgctcttc agccaattgg
     2941 aggcgatgag gggccggaga cggtcgagcc accccggcac ctcgcgcggc gcgagctcgg
     3001 tttccttgcc gcggcggtaa ggaccgagct ttccccagct caaatcgacc gcgaccacca
     3061 gatcggcgcc gagagcccgg cacatcgaca ccggcaccgg attgacgagc ccgccgtcaa
     3121 ccaataccgc accctcgcgc accaccggcg tgagcagccc aggcatggcg caggaagcac
     3181 gcaccgcatc gagcaccttg ccgtggcgca gccaaagctc tctgcccgaa gcgagctcgg
     3241 tggcgatcgc tccgaacggc aggggcagct cgtcgatctc gcgatcctcg agcagcgcgc
     3301 ggaacagcgc gaaaagcctc gtgcctcgaa tcagcccgcc gcgccacgtc aggtccatca
     3361 ggcgcaccac ggtggcccag ctgaggctgc aggcccattt ctccagccgg tcgagatctc
     3421 ccgaagcata ggccgcgccc accagcgcgc cgatcgagct gccgcatacc atgtcgggct
     3481 tgatcccgcg ctcttccagc gcgcggatca cgccgatgtg cgcccagccg cgcgcgctgc
     3541 ccgagccgag cgcgagtccg aggcggagct tgcgccgcat gcgccgcagt ctaagggatc
     3601 aatggagtaa caacccggat acaagccgca acggatctta aattcttgcg cgcggtgcgc
     3661 gccgctagca tcgccagcca tgaatagttc cttggaagcg actgcgccca tggggggcgc
     3721 gatctcgcgt cccgcgcccg catccgccgc gcacgccagg gtgcggctcg attcgatcga
     3781 cctgctgcgc gggctggtaa tggtcatcat ggtg
//
LOCUS       DASUNT010000148         2129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1160172, whole genome shotgun sequence.
ACCESSION   DASUNT010000148 DASUNT010000000
VERSION     DASUNT010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160172"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VFX94_02895"
     CDS             complement(<1..652)
                     /locus_tag="VFX94_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5767157.1"
                     /translation="MQEVYEPARVEREAQAFWASRDSFRSTEDRARPKFYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLTRYYRMRGYNVLQPMGWDAFGLPAENAAMANSVPPAKWTY
                     DNIAYMKSQLRSLGFALDWSRELATCQPGYYRWNQWLFTRLFRKGLAYKKTGVVNWDP
                     VDQTVLANEQVIEGRGWRTGAIVEKREIPMYFLRITAYAEELLAALDELPGWPEQVK"
     gene            745..1602
                     /locus_tag="VFX94_02900"
     CDS             745..1602
                     /locus_tag="VFX94_02900"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX5767158.1"
                     /translation="MKALLVALVLGLASTQPLAQPAAVIEGVQMPAWRERDGKRVPLV
                     PGMELRAGDRIVSGKEARVVVRLAEGSMVRLGEDGTLRFARIEPSREIFRAALEVLQG
                     AFRFTTDVAAKARKREVSVRVDTVTIGVRGTDFWGRSRAELQVVCLIEGAIEVGAQGE
                     QPVPMDQPRQFYRRDKGKTQPVGFVEPKQLRQWALETDIEAGKGALRQGGRFSVALLA
                     AAEQSQANSVRNQLREAGYPAEVASSKEGDKTTYLVRIRHLPSREEAQALANQLKGRF
                     GIREPKVSG"
     gene            complement(1609..>2129)
                     /locus_tag="VFX94_02905"
     CDS             complement(1609..>2129)
                     /locus_tag="VFX94_02905"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HEX5767159.1"
                     /translation="EMRTPLHCRFVDDSGTGYLPVYPEVTARYTEERDGPWTGHLGTP
                     RALCIERILRIADEFKVFNRFYVDATRLPAFGTLPAKKLSGQNFKEIIWRESGLQIDR
                     LAQFLSLVKPPQAICRAIGVRGGTSVTRLDSFGFSGKENPLYYQENYIPPNTRRLHVA
                     TDGRDAGLGRSG"
BASE COUNT          364 a          678 c          737 g          350 t
ORIGIN      
        1 gcttcacctg ctccggccag ccgggcagct cgtcaagcgc ggcgagaagc tcctcggcgt
       61 aggccgtaat gcgcaggaag tacatcggga tctcgcgctt ctcgacgatc gcgcccgtgc
      121 gccagccgcg gccctcgatc acctgctcgt tcgcgagcac cgtctggtcg accgggtccc
      181 agttcaccac ccccgtcttc ttgtaggcaa ggcccttgcg gaaaagccgc gtgaagagcc
      241 attggttcca gcggtagtac cccggctggc aagtcgcgag ctcgcggctc cagtcgagcg
      301 caaatcccag gctccgcagc tgggacttca tgtaggcgat gttgtcgtag gtccatttcg
      361 ccggcggcac gctgttcgcc atcgcggcat tctcggccgg caggccgaac gcgtcccagc
      421 ccatcggctg cagtacgttg tagccccgca tgcggtagta gcgcgtcagc acgtcgccga
      481 tggtgtagtt gcgaacgtgc cccatgtgca gcttccccga ggggtaggga aacatggaca
      541 gacagtagaa tttcggtctg gcgcgatctt cggtcgagcg gaaggaatcg cgacttgccc
      601 agaacgcctg ggcctcgcgc tccactcttg ccggttcgta gacctcttgc atgcgaagtt
      661 cttccgcgga agccgcggat tatcgcatcc aacgcaagca gcgcatccgt agatgacagg
      721 caatgcagga ggcggggata cttgatgaaa gcgctactcg ttgctctcgt cctcggtctg
      781 gcgtcgaccc agccgctcgc gcagccggcc gcggtaatcg agggcgtgca gatgcccgcc
      841 tggcgcgaac gcgacggcaa gcgcgtgccg ctcgtgccgg gaatggagct gcgcgccggg
      901 gaccggatcg tgagcggcaa ggaggcacgc gtggtggtgc gcctcgccga gggcagcatg
      961 gtgaggctcg gcgaagacgg cacgctgcgc tttgcccgga tcgaaccgtc gcgggaaatc
     1021 ttccgcgccg cgctcgaggt actgcagggc gcgtttcgtt ttacgaccga cgtggcggcg
     1081 aaggcgcgca agcgcgaggt gagcgtgcgt gtcgacaccg tgacgatcgg cgtacgcggc
     1141 accgatttct ggggccgctc gcgcgccgag ctccaggtcg tgtgcctgat cgagggcgcg
     1201 atcgaggttg gggctcaggg ggagcagccc gtgccgatgg accagccgcg gcagttctac
     1261 cggcgcgaca agggaaaaac ccagccggtc ggcttcgtcg agccgaagca gctccggcaa
     1321 tgggcgctcg agaccgacat cgaggcgggc aagggcgcac tgcgccaggg cgggcgcttc
     1381 agcgtcgctc tgctcgcggc cgccgagcag tcgcaggcga acagcgtcag gaaccagctg
     1441 cgcgaagccg gctatcccgc ggaagtcgcg agcagcaagg aaggcgacaa gaccacctac
     1501 ctcgttcgca ttcgccacct gccctcgcgc gaagaggcgc aggcgctggc caaccagctc
     1561 aaaggccggt tcggcatccg cgagccgaaa gtctcgggct agagacggtc agcccgagcg
     1621 gccgagcccg gcgtcgcggc cgtcggtcgc gacgtgcaga cgtcgagtgt tcggcggaat
     1681 gtagttttcc tggtagtaga gcgggttttc cttgcccgag aagccgaacg agtccaggcg
     1741 ggtgacgctg gtgccgcctc tcacgccgat cgcgcggcag atcgcctgcg gcggcttcac
     1801 gagcgagagg aactgggcga gtcggtcgat ctgcaagccg ctttcgcgcc agatgatctc
     1861 cttgaagttc tggcccgaaa gtttcttcgc cggcaacgta ccgaaggccg ggaggcgcgt
     1921 ggcgtcgacg tagaagcggt tgaacacttt gaactcgtcg gcaatgcgca gaatgcgctc
     1981 gatgcaaagt gcccgcggcg tgcccaagtg gccggtccat gggccgtcgc gctcctccgt
     2041 gtagcgcgcc gtaacctccg ggtataccgg gagatacccg gtgccggaat cgtcaacgaa
     2101 gcggcagtgc agcggcgtgc gcatctctc
//
LOCUS       DASUNT010000149         2810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_458692, whole genome shotgun sequence.
ACCESSION   DASUNT010000149 DASUNT010000000
VERSION     DASUNT010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458692"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2248)
                     /gene="ligD"
                     /locus_tag="VFX94_02910"
     CDS             complement(<1..2248)
                     /gene="ligD"
                     /locus_tag="VFX94_02910"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX5767160.1"
                     /translation="MADRLERYREMRDFEVTPEPRGKKLRARKNRLRYFIQRHDATRL
                     HYDVRLELEGVLKSWAVPKGPSLDPADKRLAMQTEDHPLEYGEFEGVIPEKQYGAGEV
                     VLWDRGVWTPEDKDPAAALAKGRLHLRLEGEKLRGSWILTRTSKGEERSSWLMIKRHD
                     AAARPGVDITVERPESVKRAPRTAKRASRAELPQFVTPQLATLVSQPPKSGDWVYEVK
                     HDGYRMLARLSANEVRLFTRSGREWTQKLPQLARELGKLGLDGAWLDGEIVVLRPDGR
                     SSFQALQNAFEGGADSKIVYYVFDAPFLEGRDLRQLALRERKARLRKALQPGGMVRFS
                     EDLAGSAQEILENACKLGLEGLIGKEAGSVYVPGRTRSWIKLKCRLTQDFVIAGYTRP
                     GGSRQGFGALVLGVYERPGKLVYAGKVGTGFNDELLSDLSRKFSRLKRSDSPLENPPR
                     ERDIQWLRPVLVAQVEYTERTDDGVIRQGSFMGLREDIPAKSVGMEAPQAAPERASAR
                     TMKITHPDRLIWPALGIRKIDLVEYFEQVGEWFLPHVAGRPLTLVRCPDGVEKKCFYQ
                     RHLNMGASPGDVLTFKRLRSSKGYYIYVNRLQGVISAVQNGAVEFHTWGATVPEAQHP
                     DRITLDLDPDPDLPWQQVMEGATLTRALVEGLNLRCFLKTTGGKGLHIVFPIERRNTW
                     DEVKAFAESIAGFLVRAEPGRFTAKIAKQSRGGKIFVDYLRNAETASAVAAYSPRARP
                     GAHVSTPLD"
     gene            2380..>2810
                     /locus_tag="VFX94_02915"
     CDS             2380..>2810
                     /locus_tag="VFX94_02915"
                     /inference="COORDINATES: protein motif:HMM:NF016827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX5767161.1"
                     /translation="MGPAIALRLRQHLTLTPQVQQALRLLQMSAMEFAQEMEQALAAN
                     PFLEENPDAPPAPRDTPLEEVVLAQEGSGMAPPSEREQDDWGGVSDAQPTLSEHLREQ
                     LRMSRVSDRDRALAEMVIDSLDDDGYFKASFEELCALLPTE"
BASE COUNT          432 a          976 c          911 g          491 t
ORIGIN      
        1 agtcgagcgg cgtggaaacg tgcgcgcccg gccgggcgcg aggcgagtag gcggccaccg
       61 cgctcgcggt ctcggcgttg cgcaggtaat cgacgaagat cttgccgccg cggctctgct
      121 tggcgatctt cgcagtaaag cgccccggct cggcgcgcac gaggaaaccg gcgatgctct
      181 cggcgaacgc cttcacctcg tcccacgtgt tgcgccgctc gatcgggaag acgatgtgca
      241 gccctttgcc gccggtggtc ttgagaaagc agcgcagatt cagtccttcg accagcgcgc
      301 gcgtgagggt cgctccttcc atcacctgct gccaaggcag gtcgggatcc gggtcgaggt
      361 cgagggtgat gcggtccggg tgctgcgcct ccggcactgt ggcgccccag gtgtggaatt
      421 ccaccgcgcc gttctgcact gcgctgatca ccccctgcag cctgttcaca tagatgtagt
      481 agcccttgct cgagcgcagc cgcttgaacg tcagcacgtc gcccgggctt gcgcccatgt
      541 tgaggtggcg ctggtaaaag cacttctttt ccacgccgtc ggggcagcgc acgagcgtga
      601 gcggccgacc ggccacgtgc ggcaggaacc attcgccgac ctgctcgaaa tactcgacga
      661 ggtcgatctt gcggatgccg agcgccggcc agatcagccg gtcgggatgc gtgattttca
      721 tggtcctggc actcgcacgc tccggggcgg cctgcggcgc ctccatgccg accgacttgg
      781 ccgggatgtc ctcgcgcagc cccatgaacg agccctgccg gatcacgccg tcgtcggtgc
      841 gctcggtgta ctcgacctgc gcgacgagga ccggccgcag ccactggatg tcgcgctcgc
      901 gcggcggatt ttcgagcggg ctatcgctcc tcttcagcct ggagaacttc cgggaaaggt
      961 cgcttagcaa ttcatcgttg aaccccgtgc cgaccttgcc ggcatagacg agctttcccg
     1021 ggcgctcgta gaccccgagc acgagcgcgc cgaagccctg gcgcgagccc cctggcctgg
     1081 tatagcccgc gatgacgaag tcctgcgtca ggcggcactt gagcttgatc cagctgcgcg
     1141 tgcgcccggg gacgtacacc gacccggctt ccttgccgat gagaccttcg aggccgagct
     1201 tgcaggcgtt ctccaggatc tcctgcgcgc tacccgcgag atcttcgctg aagcgcacca
     1261 tgccgcccgg ctgcagagcc tttcgcaagc gcgctttgcg ttcccgcaac gccaattgcc
     1321 gaaggtcgcg cccttccagg aaaggcgcgt cgaagacgta gtagacgatc ttcgagtcgg
     1381 cgccgccctc gaacgcgttc tgcagcgcct ggaagctcga gcgcccgtcg gggcgcagca
     1441 ccacgatctc gccgtcgagc cacgcgccgt cgagaccgag cttgccgagc tcgcgcgcga
     1501 gctgaggcag cttctgcgtc cactctcttc cgctccgcgt gaagagccgc acctcgtttg
     1561 cggagagcct ggcgagcatc cggtagccgt cgtgcttcac ctcgtacacc cagtcgccgc
     1621 ttttcggagg ctggctgacg agcgtagcga gctgcggggt gacgaactgc ggaagctcgg
     1681 cacggcttgc gcgctttgcg gtcctgggcg cgcgcttcac gctctcgggc cgctcgacag
     1741 tgatgtcgac gccgggccgc gccgccgcgt cgtgcctctt gatcatgagc cacgacgagc
     1801 gctcctcgcc tttgctcgtt cgcgtcagga tccatgagcc gcgaagcttc tcaccttcga
     1861 gccgcaagtg caggcgcccc ttggcgagcg cagcggccgg gtccttgtcc tcgggagtcc
     1921 acactcccct gtcccacaac acgacctcgc cggctccata ctgcttttcc gggatcacgc
     1981 cctcgaactc gccgtactcg agcggatggt cctcggtctg catggcaagg cgcttgtcgg
     2041 cggggtcgag gctcgggccc ttcggcaccg cccagctctt cagcacgcct tcgagctcca
     2101 ggcgcacgtc ataatgcagc cgcgtcgcgt catggcgctg gatgaagtac cgcagccggt
     2161 tttttcgggc ccgcagcttc ttcccgcgcg gctcgggcgt gacttcgaag tcccgcatct
     2221 cccggtagcg ctcgaggcga tcggccatgc ggcgggcgat ctgcaaggcg cgagcccgta
     2281 cggtaattgc tacgccgcac gcgcaattat tacagcgagc ggcctgggtc ccttgggggc
     2341 aagcgtcctg gcatggcctt tgcagatttc cggccagaca tgggaccggc aatcgcatta
     2401 cggctgcggc agcacctcac gctcacaccg caggtccaac aagcgctgcg cctgctccag
     2461 atgtcggcga tggagtttgc ccaggagatg gagcaggcgc tcgccgccaa tcccttcctc
     2521 gaagaaaacc cggacgctcc ccccgccccg cgcgacaccc cgttagaaga agtggtcctt
     2581 gcacaggaag gctcgggaat ggcgccgccg tcagagcgcg agcaggacga ctggggcggc
     2641 gtgtccgacg cgcagccgac gctgtcggag cacctgcgcg agcagctgcg catgtcgcgc
     2701 gtgagcgacc gcgaccgcgc gctcgccgag atggtgatcg acagcctcga cgacgacggc
     2761 tacttcaagg cgagcttcga ggagctctgc gcccttctgc ccacggagca
//
LOCUS       DASUNT010000150         2979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_634073, whole genome shotgun sequence.
ACCESSION   DASUNT010000150 DASUNT010000000
VERSION     DASUNT010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634073"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFX94_02920"
     CDS             <1..430
                     /locus_tag="VFX94_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX5767162.1"
                     /translation="ALLEQKKALEKELGRLRSRLALGQGQDLAGPAVDVKGAKVLVAT
                     LDGADAKTLRETMDKLKDRLKSAAIVLGSVQDGKVALIAGVTPDLTGKLKAGELVNYV
                     AQQVGGKGGGRPDMAQAGGTEPAKLPVALQSVKSWVEQRV"
     gene            427..771
                     /locus_tag="VFX94_02925"
     CDS             427..771
                     /locus_tag="VFX94_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HEX5767163.1"
                     /translation="MNRIAIVVAALITPTFSMAHHGWSEYDSANELKLTGKIIESGYE
                     HPHGHVRLEASGKTWEVVLAPPSRMERRGLEKDALKPGVTATVVGYPNRNKPEEMRAE
                     RIIINGKTVELR"
     gene            768..1271
                     /locus_tag="VFX94_02930"
     CDS             768..1271
                     /locus_tag="VFX94_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767164.1"
                     /translation="MTSSGGGPLGALEASGVGQAMRQWLWLYPSVEIVHILGIGLLFG
                     SIAVLDLRLLGFSRSISVRRLASHVLPWTAASFLLIIPSGLAMFTAHATEFVQSEVFV
                     LKMLLILAAGLNAALFHTITFRTAEVWDSEEMRQLAPPPSARAAGAISIALWISVIAC
                     GRLLAYF"
     gene            complement(1268..2221)
                     /locus_tag="VFX94_02935"
     CDS             complement(1268..2221)
                     /locus_tag="VFX94_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004537763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5767165.1"
                     /translation="MAIPIAGFKDLYDVASVEKALQELAPSANEALRALYEKMVRLGG
                     QRFTVKPSALPEMERLFAELPNFAEVLADIRKHLALCIDSNDSIELPPMLLLGEPGIG
                     KTHFARRLAELLGTGFGFVPMSSLTAGWVLSGASSQWKNAKPGKVFDTFVNGDYANPV
                     IVVDELDKASGDSQYDPLGALYELLEVKTATRFVDEFAELPIDASGAVWLATANDAGR
                     IPDPLLDRLTVYQIEPPDAAGACSIAAAIYREIRNSHDWGRQFPEAPSESALDKLASM
                     PPREMRRALQGAFGTAKLAGRSELGPDDMQDPRSKKQRIGF"
     gene            2336..2761
                     /locus_tag="VFX94_02940"
     CDS             2336..2761
                     /locus_tag="VFX94_02940"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5767166.1"
                     /translation="MTALTREHFNGFGQGYLPGLTGVEIVTVSREAVESRMPVRREVM
                     APNGFLHAASVIALADTSCGYGCIANLPEGARGFTTIELKANFLGTAREGAIACRASA
                     VHLGRTTQVWDAVVTNEATGAKIALFRCTQMVLWQNQKP"
BASE COUNT          521 a          963 c          990 g          505 t
ORIGIN      
        1 cgcgctgctc gaacaaaaga aggcgctcga gaaggagctc ggccgcctgc gctcgcgcct
       61 tgcgctcggg cagggccagg accttgccgg gccggcggtg gacgtgaaag gcgcgaaagt
      121 gctggtcgcc actctcgacg gcgcggacgc caagaccctg cgcgagacca tggataagct
      181 gaaggacagg ctgaaatccg cggcgatcgt cctgggctcg gtgcaggacg gcaaggtcgc
      241 cctgatcgcg ggcgtaactc ccgatctcac cgggaaactc aaggccggcg agctggtcaa
      301 ctatgtggcg cagcaggtgg gcggcaaggg gggcgggcgg cccgacatgg cgcaggccgg
      361 cggcaccgag cccgctaagc tgccggtggc gctgcaatcc gtcaaatcat gggtagagca
      421 aagggtatga atcgaattgc catagtagtc gctgcactga tcacaccgac cttctccatg
      481 gcacatcacg gctggagcga gtacgactcg gcgaatgagc tgaagctgac cggcaagatc
      541 atcgagtccg gctacgagca cccgcacggc cacgtgcggc tcgaggcgtc gggcaagacc
      601 tgggaggtgg tgcttgcgcc gccttcgcgc atggagcgcc gcggcctgga aaaagatgcg
      661 ctgaagcccg gtgtcaccgc caccgtcgtc ggttatccca accgcaacaa gccggaagag
      721 atgcgcgccg agcgcattat catcaacggg aagaccgtag agcttagatg acgtcgtcgg
      781 ggggcgggcc gctcggcgcg ctggaagctt ccggcgtcgg ccaggccatg cggcagtggc
      841 tgtggctcta cccgagcgtc gagatcgtcc acatcctggg aatcgggctg ctcttcgggt
      901 cgatcgcggt gctcgacctg cggctgctcg gcttctcccg cagcatttcc gtaaggaggc
      961 tcgcgagcca tgtgctgccc tggaccgcgg cgagcttcct cctcatcatc ccgagcggcc
     1021 tcgccatgtt caccgcccac gccaccgaat tcgtgcagag cgaggtgttc gttctgaaga
     1081 tgctgctcat tctggcggcc gggctcaacg cggcgctctt ccacacgatc acgttccgca
     1141 ccgccgaggt gtgggattcc gaagagatgc gccagctcgc gccaccgccg tcggcgcgcg
     1201 cggcgggggc gatttcgatc gcactgtgga tttccgtgat cgcctgcggg cgattactcg
     1261 cgtacttcta gaacccgatt ctttgcttct tcgagcgcgg gtcctgcatg tcgtcggggc
     1321 cgagctcgga gcgcccggca agcttcgcgg tcccgaacgc gccctgcagc gcgcggcgca
     1381 tctcgcgcgg tggcatcgac gccagcttgt ccagcgccga ttccgacggc gcctcgggga
     1441 actggcggcc ccagtcgtgc gagttgcgga tctcgcggta gatcgccgcg gcaatcgagc
     1501 acgcgcccgc agcgtcgggc ggctcgatct ggtagacagt cagccggtcg agcagcgggt
     1561 cggggatgcg gcctgcgtcg ttcgccgtgg cgagccacac cgcgccggag gcgtcgatgg
     1621 ggagctccgc gaactcgtcg acgaagcgcg tggcggtctt gacctcgagc agctcgtaga
     1681 gcgcgccgag cggatcgtac tggctgtcgc cgctcgcctt gtcgagctcg tccaccacga
     1741 tgaccggatt ggcatagtcc ccgttcacga aggtgtcgaa caccttgccg ggcttcgcgt
     1801 tcttccattg cgacgaggcg cccgacagca cccagccggc ggtgagcgag ctcatcggca
     1861 cgaagccgaa gccggtgccg agcagctcag cgagcctgcg cgcgaaatgc gtcttgccga
     1921 tgcccggctc gccgagcagc agcatcggcg gcagctcgat cgagtcgttg gaatcgatgc
     1981 acagcgcgag gtgcttgcgg atgtcggcca gcacctcggc gaagttcggc agctcggcaa
     2041 agagccgctc catctccggc agcgccgagg gcttgacggt gaagcgctgg ccgcccaggc
     2101 ggaccatctt ctcgtacagg gcccgcagcg cctcgttcgc gctcggcgcg agctcctgca
     2161 gcgccttttc gaccgaagcg acgtcgtaga ggtccttgaa ccccgcgatc ggaatggcca
     2221 cgttagccgg catgttcaag caaaaaaact agcacgatgc tttcgggcaa gcaaacaccc
     2281 tgcctggcaa aggcttgccg ggagtgctaa tcgagtagag tagttctcca cgaccatgac
     2341 tgcgctaacg cgcgagcact tcaatggttt cggccagggc taccttcccg gactgaccgg
     2401 cgtggaaatc gtcacagttt cgcgcgaagc ggtcgaaagc cgcatgccgg tgcgtcggga
     2461 agtgatggcg ccgaacggct ttttgcacgc cgcctcggtc atcgcactcg ccgacacgag
     2521 ctgcggctac ggctgcatcg cgaacctgcc ggaaggcgcc cgggggttta ccaccattga
     2581 gctcaaggcg aacttcctcg gcaccgcgcg cgaaggcgcg atcgcctgcc gcgcgagtgc
     2641 tgtccatctc gggcgcacca cccaggtatg ggacgccgtg gtcactaacg aggcgaccgg
     2701 ggcgaagatc gcgctattcc ggtgcacgca gatggttttg tggcagaatc agaagccgtg
     2761 acgcgccggt ctttctttgg cttcttcttt tactttccct gcctgctggc ggaagggacc
     2821 ggctaagctc taaccccgaa tccaaccaaa aaccgccagt gctacagaag cctggcggtt
     2881 tttttttgcg cagaggcctt atgaacgagt ccatcgacga caaaacctcc ctgaccgacg
     2941 acgagcgcgt cgagaacgtc gtgccgctgc cgccgccgg
//
LOCUS       DASUNT010000151         6752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_19935, whole genome shotgun sequence.
ACCESSION   DASUNT010000151 DASUNT010000000
VERSION     DASUNT010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19935"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..467
                     /locus_tag="VFX94_02945"
     CDS             54..467
                     /locus_tag="VFX94_02945"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5767167.1"
                     /translation="MARIPEGFRKIERLSPFNALVGPLYERRDGEAVSIGLAIKEKHT
                     NSRGICHGGVLATLADLALGYAMLARSGDRGSFLTAHLAVDYAGAARPGDFIESRVEV
                     QHVGSRLAFANCYLVARGRPIVRASAIFARGDKSG"
     gene            464..1069
                     /locus_tag="VFX94_02950"
     CDS             464..1069
                     /locus_tag="VFX94_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5767168.1"
                     /translation="MTPRHVVLVPGLWMPSAAMAPLSLRLRRQGYEVHVFAYYGRAPH
                     ESNVEALARFSRGRTGFVGHSLGGLLILDMLNRYPEIPVDAAVLLGAPVRGCLAGRRF
                     GGARLGRWMMGACSSLWEERAARWKRRAPLGVVAGTLPLGLARAFGTLPGPNDGVVRV
                     EETTVDGMTEQALVRQGHSMLVVSRQVSELSERFLAQGRFA"
     gene            1066..2073
                     /locus_tag="VFX94_02955"
     CDS             1066..2073
                     /locus_tag="VFX94_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX5767169.1"
                     /translation="MRRWVFVSIFVLALVAGCQSLAYYSQAINGHFHVLSKARPVSDW
                     IADPATPPELKARLETARRIREFASKSLALPQNGSYLSYADIERPYVVWNVFAAPEFA
                     VEAKIECFPFAGCVSYRGFYSEHEARRHAAKLRAEGYDVHVGGVPAYSTLGWSDDPLL
                     STFIRYPDAQLARLVFHELAHQVVYARNDTTFNESFAVTVEEEGVRRWLEAENRAGEL
                     ATFASAQARKRDLAERVSRARERLKSVYASDLTRERKLEEKSAEWQRLREAFPALPAE
                     PNNAFLASIAVYTELVPAFERLLAESGSLDAFYRRVKDLARDPSARETYFTTAGYRRS
                     S"
     gene            complement(2042..2659)
                     /locus_tag="VFX94_02960"
     CDS             complement(2042..2659)
                     /locus_tag="VFX94_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX5767170.1"
                     /translation="MLKIWGRVNSVNVKKVLWAAEELGLKYERIDAGREFGVVNTPEY
                     RRMNPNSLVPTIDDNGFVLWESHAIVRYVAAKHGAGTLWPVDLRQRADADRWMDWAFT
                     FQASLRAVFWNLVRTPPEKRDMQAVEEGRKKCAELLAIPDAALANRPYFAGASLTIGD
                     IPLGCHVQIWMRLPIERPAHPNLSRWFDRLCARAPFKKIVDIPLS"
     gene            complement(2670..4256)
                     /locus_tag="VFX94_02965"
     CDS             complement(2670..4256)
                     /locus_tag="VFX94_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX5767171.1"
                     /translation="MTAFAWNNPYPWPRKPLLAANVVATSQPLAAQAGLQMLAAGGSA
                     VDAILAAAITLTLVEPVSNGIGSDAYAIVWDGERLHGLNASGRSPAGWTPEFFACAGR
                     VPERGWNSVSVPGCVSAWVELHRRWGKLAFEKLFDRAIEYGRRGFLVSPTIAGQWEKQ
                     VTELKEQPGFADAFLPDGRPPRPGQRFTFEKHAATLERIRDTRGEAFYRGELAEKLEA
                     HARKHGAAMRASDLAAHQSDWVEPLSMDYRGYTLHEIPPNGQGIVTLMALGMLEHFDM
                     RSHPVDGADSLHLQIEAQKLAFADARRYVADIDYMQEVRPAALLDKLYLKERARLIDL
                     KRAQDFGHGTPPKSGTVYLTAADASGLMVSFIQSNFMGFGSGVVVEGISLQNRGATFV
                     LEKGHPNCVGPRKRPYQTIIPGFVTRAGKPVMSFGVMGGTMQPQGHAQVMVRIADYGQ
                     SPQAACDGPRFRVVQAMDVSVETHFDPAVIEELKRRGHRIVSLPDDYNQFGSAQLIWK
                     LDGGYFTASDPRRDGQAVGF"
     gene            complement(4267..>6752)
                     /locus_tag="VFX94_02970"
     CDS             complement(4267..>6752)
                     /locus_tag="VFX94_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX5767172.1"
                     /translation="RGCLSANPKSPDVTTRMLREHTEAHARASRAAPRFTWPAPPYFE
                     YVDAAWHNDECMLVFRDGAKASGRLIDLLPDAAELKFRSHDAADIVSIRFSSLLMMQF
                     PHPVALRRQPVKIDDTEELYPASARQPFAIGLVNGKTSLGETVGSVHALCGLFLFPPD
                     QDDTMVTRWFVPAEAIRSLSVGKPLGEMLVDERLASPEVVDAALSKQHEMRSRRFGDY
                     LTEHQIVSPEQLAAALKQQRAQPMQKLGEALVELGYLTEAELEEALAIEARDRSVPLG
                     RILADMGVVDAEIVNAVMARKLGIPFVNLKDFRIPPAVLKRIPSAAAHRFNTVPVAEA
                     ENGLVVAVENPMDMAKLEGLRFVAGMKLLPVMASAEDIRAALERAYGPAHGSGVAAAP
                     RKAADVRSISEITHRLSADIADADLDEQQSVAHDSALVQLINKMIIDASEQKASDIHI
                     EANPAGRNMRIRFRKDGALVPYLDLASRFRKAVVSRIKVMSQLDITERRKPQDGKIEF
                     RRFGPLDVDLRVATIPTSGGLEDVVMRVLGAATPVPMAELGFEASTLEQVKRLISRPH
                     GLFLVCGPTGSGKTTTLHSLLASINTADTKIWTAEDPIEISQPGLRQVQVNPKIGWTF
                     AAAMRSFMRADPDVIMVGEMRDPETAKIGIEASLTGHLVLSTLHTNSAPESVTRLLDL
                     GMDPFNFADALLGVLSQRLVRKLCTNCRIAYTPGTAELEELAAEYCGGSRGDAAKVVH
                     TWRSQPKGITLYRAKGCGRCDRTGYKGRLGIYELLVADPAIKRLIQSRAPVAEIAVAA
                     AAQHLRTLKQDGIDKVVRGLTDLHQVHTV"
BASE COUNT         1006 a         2265 c         2322 g         1159 t
ORIGIN      
        1 gcctgcactc gcggcaggcg tgcgcctcgc ctcgacggct agaatggccc gcaatggccc
       61 gcatccctga aggatttcgc aagatcgagc gcttgagccc cttcaacgcg ctcgtcgggc
      121 cgctctatga gcgccgcgac ggggaggcgg tgtcgatcgg cctcgccatc aaggaaaagc
      181 acaccaactc gcgcggcatc tgccacggcg gcgtgctcgc cacgctagcc gacctggcgc
      241 tcggctatgc gatgctcgcc aggtcgggcg acaggggaag cttcttgacc gcccacctcg
      301 ccgtggacta cgccggcgcc gcgcgccccg gcgatttcat cgagtcccgc gtcgaggttc
      361 agcacgtcgg ctcgcgcctc gcgttcgcga actgctatct cgtcgcccgt gggaggccca
      421 tcgtgcgggc gagcgcgatc tttgcccgcg gggacaagag tggatgacgc cgcgccatgt
      481 ggttctcgta ccgggcttgt ggatgccgtc ggcggcgatg gcgccgcttt cgctgcgctt
      541 gcggcggcag ggctacgagg tgcacgtctt cgcctactac gggcgcgctc ctcatgaatc
      601 gaacgtcgag gcgcttgcgc gcttttcccg cggcaggacc ggtttcgtgg gccacagcct
      661 gggcggcttg cttatcctgg acatgctgaa ccgctacccc gaaatcccgg tcgatgccgc
      721 cgtgctgctt ggcgccccgg tgcgcggctg cctcgccggg cgccggttcg gcggcgcgag
      781 gctcgggcgg tggatgatgg gtgcctgctc ctcgctgtgg gaggagcgcg cggcgcgctg
      841 gaagcgccgg gcgccgctcg gggtcgtcgc cggcaccttg ccgctggggc tcgcccgcgc
      901 tttcggtacg ctgccgggtc cgaacgacgg cgtggtgcgc gtcgaggaga cgacggtgga
      961 cgggatgacg gagcaggcgc tcgtgcgcca ggggcacagc atgctcgtgg tgtcgcgcca
     1021 ggtcagcgag ctgtcggagc gttttctcgc ccaggggcgt ttcgcatgag aaggtgggtc
     1081 ttcgtctcga ttttcgtcct cgcgcttgtc gcggggtgcc agtcgctcgc ctactacagt
     1141 caggccatca acggccattt ccacgtgctg tccaaggcgc ggccggtgtc ggactggatc
     1201 gccgacccgg cgacgccgcc agagctcaag gcgcggctcg aaaccgcgcg ccgcatccgc
     1261 gagttcgctt cgaagagcct cgcgctgccg cagaacggca gctatctttc ctacgccgac
     1321 atcgagcgtc catacgtcgt ctggaacgtg ttcgcggcgc ccgagtttgc ggtcgaggcg
     1381 aaaatcgaat gcttcccctt tgccggctgc gtgagctatc gcggctttta ctcggagcac
     1441 gaggcgcgcc gccatgccgc gaagctgcgc gcggaaggct acgacgttca tgtcgggggc
     1501 gtgcctgcct attccacgct cggctggagc gacgatccgc tgctttccac cttcatccgc
     1561 tacccggacg cgcagctcgc ccggctcgtg ttccacgagc tcgcccacca ggtggtctac
     1621 gccaggaatg acaccacctt caacgagtcg ttcgccgtga ccgtggagga ggaaggcgta
     1681 cggcgctggc tggaggcgga gaatcgcgcg ggcgagctcg ccacattcgc gtccgcgcag
     1741 gcgcgcaagc gagacctcgc cgagcgcgtg agccgggcgc gcgagcgcct gaaatccgtg
     1801 tatgcgtcgg atctcacgcg tgagcgcaag ctcgaggaga aaagcgccga gtggcagcgg
     1861 ctgcgcgagg cgttcccggc gctgcccgcg gagccgaaca acgccttcct cgcctcgatc
     1921 gccgtctaca ccgagctcgt gccggcgttc gaacggctgc tcgccgaaag cggcagcctg
     1981 gacgcgttct accggcgggt gaaggacctg gcgcgcgatc cgtcggcgcg cgaaacctac
     2041 ttcacgacag cgggatatcg acgatcttct tgaacggggc ccgcgcgcac agccggtcga
     2101 accaccggct cagattcggg tgtgccgggc gctcgatcgg cagccgcatc cagatctgca
     2161 cgtggcagcc gagggggatg tcgccgatcg tgaggctcgc gccggcgaag tagggccggt
     2221 ttgcgagcgc cgcgtccggg atcgcgagca gctcggcgca cttcttgcgc ccttcttcga
     2281 ccgcctgcat gtcgcgcttc tccggtggtg tgcgcaccag gttccagaac accgcgcgca
     2341 gcgacgcctg gaaagtaaag gcccagtcca tccagcggtc cgcatcggcg cgctgcctga
     2401 ggtccacggg ccacagcgta cccgcgccgt gctttgccgc gacgtagcgc acgatcgcgt
     2461 gcgattccca cagcacaaag ccgttgtcgt cgatggtcgg cacgagggag ttcggattca
     2521 ttctcctgta ctcgggcgta ttcaccacgc cgaactcgcg gccggcgtcg atgcgctcgt
     2581 atttcagccc gagctcctcg gcggcccaca gcaccttctt cacgttgacc gaattgacgc
     2641 gtccccagat tttgagcatg tggcgtgtcc tagaaaccta ccgcctgccc gtcccgcctc
     2701 gggtcgctcg cggtgaagta gccgccgtcg agcttccaga tgagctgcgc gctgccgaac
     2761 tggttgtagt cgtcgggcag cgagacgatg cggtgcccgc ggcgcttcag ctcctcgatc
     2821 accgccggat cgaagtgcgt ttcgacgctg acgtccattg cctgcacgac ccggaagcgc
     2881 ggcccgtcgc atgccgcctg cgggctctgg ccgtagtccg cgatgcgcac catgacctgc
     2941 gcatggccct gcggctgcat ggtgccgccc atgacgccga agctcatcac cggcttgcct
     3001 gcgcgcgtca cgaagcccgg gatgatcgtc tggtaggggc gcttgcgcgg gccgacgcag
     3061 ttcgggtggc ccttttcgag cacgaaagtc gcgccgcggt tctgcagcga aatcccttcc
     3121 acgacgacgc ccgagccgaa ccccatgaaa ttggactgaa tgaacgaaac catgagcccc
     3181 gacgcgtcgg ccgccgtcag gtacaccgtg ccgctcttgg gcggcgtgcc gtggccgaag
     3241 tcctgcgcgc gcttgagatc gatcagccgc gctctttctt tcaagtaaag tttatccagc
     3301 agcgccgcgg ggcggacctc ttgcatgtag tcgatgtcgg cgacatagcg ccgcgcgtcg
     3361 gcgaatgcca gcttctgcgc ctcgatctgc aggtgcaggc tgtccgcgcc gtcgaccggg
     3421 tgcgagcgca tgtcgaagtg ctcgagcatg ccgagcgcca tgagcgtcac gatgccctgg
     3481 ccgttcgggg ggatttcatg cagcgtgtag ccgcggtaat ccatcgaaag cggctcgacc
     3541 cagtcggact ggtgcgcggc gaggtccgag gcacgcattg ccgccccgtg cttcctcgca
     3601 tgcgcttcca gtttttccgc cagctccccg cgatagaagg cttcgcccct tgtatcgcgg
     3661 atgcgttcca gcgttgccgc gtgtttctcg aacgtgaagc gctggccggg ccggggaggg
     3721 cgaccgtccg gcaggaacgc gtcagcgaac cccggctgct ccttcaactc cgtaacctgc
     3781 ttctcccact gcccggcgat ggtgggcgag acgaggaagc cgcgacggcc gtattcgatc
     3841 gcccggtcga agagcttctc gaaggcgagc ttgccccagc ggcggtgcag ctccacccag
     3901 gccgacacgc accccggcac gctcaccgaa ttccagccgc gctcgggcac gcgcccggcg
     3961 caggcgaaga actcgggcgt ccagccggcg ggcgagcggc ccgaggcatt caggccgtga
     4021 agccgctctc cgtcccagac gatcgcgtag gcgtccgagc cgatgccgtt cgataccggc
     4081 tccaccagcg tcagggtgat cgccgcggcg aggatcgcat cgaccgcgct cccgcccgcg
     4141 gcgagcatct gaaggcccgc ttgcgcggcg agcggctgcg aggtagcaac gacgttggcg
     4201 gcaagcagcg gcttgcgggg ccagggatac gggttgttcc aggcgaaagc ggtcaccgcc
     4261 gctagtttag acggtgtgca cctggtgcag gtctgtcagt ccgcgaacga ccttgtctat
     4321 gccgtcctgc ttcaatgtgc gcaggtgctg cgcggcggcc gctaccgcta tctcggcgac
     4381 tggagcacgc gactggatca ggcgctttat cgctgggtcc gcaacgagca gctcgtagat
     4441 tcccaggcgg cccttgtagc cggtacggtc gcagcgcccg cagcccttgg cgcggtagag
     4501 cgtgatgccc ttcggctggc tgcgccaggt gtgcacgacc ttggcggcat cgcctctcga
     4561 accgccgcag tattccgccg ccagttcctc gagctccgcc gtgccggggg tataggcgat
     4621 tctgcagttc gtgcagagct tgcgcaccag ccgctgcgag agaactccga gcagggcgtc
     4681 ggcgaagttg aacgggtcca tcccgaggtc gagaagccgc gtgacgctct ccggggcgct
     4741 attggtgtgc agggtcgaga gcaccaggtg accggtgagc gaggcctcga tgccgatctt
     4801 ggcggtctcg gggtcgcgca tctcgcccac catgatcaca tcgggatcgg cccgcatgaa
     4861 gcttcgcatc gcggcggcga aggtccagcc gattttcgga ttgacctgta cctggcgcag
     4921 ccccggctgc gagatctcga tcggatcttc ggcggtccag atcttggtgt cggcggtgtt
     4981 gatcgacgcg agcagcgaat gcagggtcgt cgtcttgccc gagccggtcg gcccgcagac
     5041 gagaaaaagg ccgtgcggcc gggagatcag gcgcttcacc tgctcgagcg tcgaggcctc
     5101 gaagccgagc tcggccatgg gcaccggcgt ggcggcgccg agcacgcgca tcaccacgtc
     5161 ctcgaggccc ccggaagtgg ggatggtggc cacgcgcagg tcgacgtcga gcgggccgaa
     5221 gcgccggaac tcgatcttgc cgtcctgcgg cttgcggcgc tcggtgatgt cgagctgaga
     5281 catcaccttg atgcgcgaca ccaccgcctt gcggaacctg gacgcgaggt cgaggtaagg
     5341 cacgagcgcg ccgtccttgc ggaagcggat gcgcatgttc cgcccggccg ggttcgcctc
     5401 gatgtggatg tcggaggcct tctgctcgct cgcgtcgatg atcatcttgt tgatgagctg
     5461 cacgagggcg ctgtcgtgcg cgacggactg ctgctcgtcg agatcggcat cggcgatgtc
     5521 ggcggagagc cggtgggtga tctcgctgat gcttcgcacg tcggcggcct tgcgcggcgc
     5581 ggcggcgacg ccgctgccgt gcgcggggcc gtacgcgcgc tcgagcgcgg cgcggatgtc
     5641 ttctgccgaa gccatgaccg gaaggagctt catcccggcg acgaagcgca atccctcgag
     5701 cttcgccatg tccatcgggt tctccaccgc gacgacgagc ccgttttccg cctcggcgac
     5761 cgggacggtg ttgaagcggt gcgcggccgc cgatggaatg cgcttcagca ccgccggcgg
     5821 gatgcggaag tccttcaggt tcacgaacgg gatgccgagc ttgcgcgcca tcaccgcgtt
     5881 cacgatctcg gcgtcgacca cgcccatgtc ggcgaggatc cggccgagcg gcacgctgcg
     5941 gtcgcgcgcc tcgatggcga gggcttcctc gagctcggct tcggtcagat agccgagctc
     6001 gaccagggcc tcgcccagtt tctgcatcgg ctgcgcgcgc tgctgcttga gcgcggccgc
     6061 gagctgctct ggcgacacga tctggtgctc ggtcaggtaa tcgccgaagc gccgcgagcg
     6121 catctcgtgc tgcttgctga gcgcggcgtc cacgacctcc ggcgaggcga gccgctcgtc
     6181 gaccagcatc tcgccgagcg gcttgccgac gcttagcgag cggatggcct ccgcaggcac
     6241 gaaccagcgc gtcaccatgg tgtcgtcctg atcgggcggg aacaggaaca gcccgcacag
     6301 cgcgtgcacc gagccgacgg tctctccaag cgacgtcttg ccgttcacga ggccgatcgc
     6361 gaagggctgg cgcgcggaag ccgggtagag ctcctcggtg tcgtcgattt tcaccggctg
     6421 acgccgaagc gccaccggat gcgggaactg catcatgagg agggaggaga aacggatgct
     6481 cacgatgtcc gcggcgtcat ggctgcggaa cttgagctcg gcagcgtcgg gcagcaagtc
     6541 gatcagcctt cccgacgcct tggcgccgtc gcggaacacg agcatgcact cgtcgttgtg
     6601 ccacgcggcg tccacatact cgaagtaagg cggcgccggc cacgtgaaac gtggcgccgc
     6661 ccggctcgcg cgcgcgtgcg cctcggtgtg ctcgcgcagc atgcgcgtgg tgacgtcggg
     6721 cgatttcggg ttggcgctca agcaaccacg cc
//
LOCUS       DASUNT010000152         4080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1072603, whole genome shotgun sequence.
ACCESSION   DASUNT010000152 DASUNT010000000
VERSION     DASUNT010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072603"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..527
                     /gene="groES"
                     /locus_tag="VFX94_02975"
     CDS             234..527
                     /gene="groES"
                     /locus_tag="VFX94_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX5767173.1"
                     /translation="MKIRPLHDRVIVKRIEEERKSAGGIVIPDTAAEKPDQGEIVAVG
                     KGKKDDNGKLIPIDVKVGDRVLFGKYSGQTVKVKGDELLVMREEDIMGVIEGA"
     gene            560..2215
                     /gene="groL"
                     /locus_tag="VFX94_02980"
     CDS             560..2215
                     /gene="groL"
                     /locus_tag="VFX94_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828028.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX5767174.1"
                     /translation="MPAKEVRFHEGARAKMVHGLNILADAVKVTLGPKGRNVVLERSF
                     GAPTVTKDGVSVAKEIELKDKFENMGAQMVKEVASKTSDVAGDGTTTATVLAQAIVRE
                     GLKYVASGMNPMDLKRGIDKAVIAVVEELKKISKPCTTSKEIAQVGAISANADPDIGK
                     IISDAMDKVGKEGVITVEDGKSLENELDLVEGMQFDRGYLSPYFINNPEKQIAVLEDP
                     YVLLHDKKISSIRELLPVLEQVAKAGKPLLIIAEEVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLEDMAVLTGGTVISEELGLKLENASLKDLGRAKKVESAKENTTIID
                     GAGEKKQIEARVKQIRVQIDEATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVAFIRVKQALEGKVKADNHDQEAGVKIVMRALEE
                     PLRIIVDNAGAEPSVVLNKVLESKGNFGFNAQTEEFGDLVQHGVIDPTKVTRTALQNA
                     SSVAGLMLTTDAMVAEMVEEKKGGGGHSHGGMPGMGGMGDMDM"
     gene            2291..2965
                     /locus_tag="VFX94_02985"
     CDS             2291..2965
                     /locus_tag="VFX94_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5767175.1"
                     /translation="MRILVAEDDAILADGVMRTLRQSGYAVDWVNNGVEADTALDTED
                     FDLLILDIGLPRKSGLDVLKRLRARDSRVPVLILTALDGVNDRVRGLDAGADDYLAKP
                     FELAELEARVRALTRRGMAGGPTLLRHGTLTYDQVGRMARLNGEPLELSAREVSLLEI
                     FLSRAGRLVSKDHLVSHLCEWGEEVSPNAIEVYVHRLRKKLEPGGVRIVTVRGLGYSL
                     EKSDSR"
     gene            2943..>4080
                     /locus_tag="VFX94_02990"
     CDS             2943..>4080
                     /locus_tag="VFX94_02990"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5767176.1"
                     /translation="MKNRTPARSPLSLLVEILDWLLAPLIILWPLSVVVTFWIANAIA
                     NIPYDRALADSVNALARHVSFNDKKGGARLALPAAASSFLRTDELDTIYFQVRGTRGE
                     VLGGDRDLAEIFPEEGQRQHIVYLRDAEWHGDPVRVAYMFMFDPQAPHRRVVVQVGET
                     LHKRQRLSNEIIAGIIIPQFFLFPVVALLAWFGLQRGTQPLERLRQRLLGRRPGDLSP
                     IDVREVPEEVAPLIEAFNGVMERLSSSMRAQERFIADAAHQMRTPLAGLRTQLEFALR
                     QGDPADLRASLERIRSSVERATRMISQLLSLARSQSETPPKFEPVDMNALAREVTQEW
                     VEPALAKRIDLGFEGSVESAVVEGSRLLLHELLNNLVDNAVRYTP"
BASE COUNT          786 a         1311 c         1362 g          621 t
ORIGIN      
        1 ttacgctgcc ctccggaacc ttcgcatcgc gcaggccctt cgaaaccgtc gcaggcaggg
       61 cggcgtgtcc tggcccgagc gcagtcagga ccaacaaaag cacgaaggtc ttgaaaacgc
      121 gccgcacggt gcttattatt agcactcgtt agaactgagt gctaagtcca gggtatccgg
      181 cttggtttca cgccaagtca ttgtaataca tcacatttta acgggagcaa tagatgaaga
      241 tccgtccgct gcatgaccgc gtcatcgtca agcgcatcga agaagagcgc aagtccgccg
      301 ggggcatcgt gatccccgac accgccgccg agaagcccga ccagggcgaa atcgtcgccg
      361 tcggcaaggg gaagaaggat gacaacggca agctgatccc gatcgacgtc aaggtcggcg
      421 accgggtgct gttcggcaag tactccggac agaccgtgaa ggtgaaaggc gatgagctcc
      481 tcgtcatgcg cgaggaagac atcatgggcg taatcgaagg cgcctgatcc gaatttcgag
      541 acactaggag accgtaacaa tgccagccaa agaagtacgc ttccacgaag gcgcccgcgc
      601 caagatggtt cacggcctga acatcctggc cgacgccgta aaagtaacct tgggcccgaa
      661 gggccgcaac gtcgtgctcg agcgctcgtt cggcgcgccg accgtcacca aggacggcgt
      721 ctcggtcgcg aaggagatcg agctcaagga caagttcgag aacatgggcg cgcagatggt
      781 gaaggaagtc gcgtccaaga cctccgacgt cgccggcgac ggcaccacga ccgccacggt
      841 gctcgcccag gccatcgtgc gcgagggcct gaagtacgtt gcctcgggca tgaacccgat
      901 ggacctcaag cgcggcatcg acaaggcggt catcgccgtg gtcgaggagc tgaagaagat
      961 ctccaagccc tgcaccacct cgaaggaaat cgcgcaagtg ggcgcgatct ccgccaacgc
     1021 ggatcccgac atcggcaaga tcatctccga tgcgatggac aaggtcggca aggaaggcgt
     1081 gatcaccgtc gaggacggca agtcgctgga gaacgagctc gacctcgtcg aaggcatgca
     1141 gttcgaccgc ggctatctct ccccctactt catcaacaat cccgagaagc agatcgcggt
     1201 gctcgaggac ccctacgtcc tgctgcacga caagaagatt tcgtcgatcc gcgagctgct
     1261 gccggtcctc gagcaggtcg ccaaagcggg taagccgctc ctcatcatcg ccgaggaagt
     1321 cgagggcgag gcgctcgcca cgctcgtcgt gaacaatatc cgcggcatcc tgaagacggt
     1381 cgcggtcaag gcgccgggct tcggcgaccg ccgcaaggcg atgctggaag acatggcggt
     1441 ccttacgggc ggcacggtga tctcggaaga gctcggcctc aagctcgaga acgcttcgct
     1501 gaaggacctc ggccgcgcga agaaggtcga gtccgccaag gagaacacga ccatcatcga
     1561 cggcgcgggc gagaagaagc agatcgaggc gcgcgtgaag cagatccgcg tgcagatcga
     1621 cgaagccacc agcgactacg acaaggaaaa gctgcaggag cgcgtggcga agctcgcagg
     1681 cggcgtggcg gtgatcaagg tcggcgccgc gaccgaagtc gagatgaagg aaaagaaggc
     1741 ccgcgtcgag gacgcattgc acgcaacccg cgccgcggtg gaagagggca tcgttccggg
     1801 cggcggcgtc gcgttcatcc gtgtgaagca ggcgctcgag ggcaaggtga aggcggataa
     1861 ccacgaccag gaagccggcg tgaagatcgt gatgcgcgcg ctcgaagagc cgctgcgcat
     1921 catcgtcgac aacgcgggcg ccgagccctc cgtcgttctg aacaaggtgc tcgagagcaa
     1981 gggcaacttc ggcttcaacg cgcagaccga agagttcggc gacctcgtgc agcacggcgt
     2041 gatcgatccc acgaaggtca cccgcaccgc gctgcaaaac gcctcctccg ttgccggcct
     2101 gatgctcacc accgacgcga tggtggcgga gatggtcgag gagaagaagg gcggcggcgg
     2161 ccattcgcac ggcggcatgc ccggcatggg cggcatgggc gacatggaca tgtaactgcg
     2221 ccgacgcgga gttaatcccc gcgcaagggg tgcttcggca ccccttttca tttgtattag
     2281 actggcccgc atgaggatcc tggtcgcgga ggacgacgcg atcctggccg acggtgtgat
     2341 gcgcacgctg cggcagtccg gctacgccgt ggactgggta aataacggcg tcgaggccga
     2401 tacggcgctc gataccgaag acttcgatct gctcatcctc gacatcggcc tgccgaggaa
     2461 atcgggtctc gacgtgctga agcgtctgcg cgcgcgcgac tcgcgcgtgc cggtgctcat
     2521 tcttaccgcg ctcgacggcg ttaacgaccg cgtgcgcggc ctcgacgcgg gcgcggacga
     2581 ctaccttgcc aagcccttcg agctcgcgga gctggaagcg cgggtgcgcg cgctgacgcg
     2641 ccgcggcatg gcgggcgggc cgacgctgct gcgccatggc acgctcacct acgaccaggt
     2701 cgggcgcatg gcgcgcctga acggcgagcc gctcgagctc tcggcgcgcg aggtgagcct
     2761 gctcgagatc ttcctctccc gcgcggggcg cctcgtgtcg aaggaccacc tcgtctcgca
     2821 cctttgcgag tggggcgagg aagtgtcgcc caatgcgatc gaggtgtacg tgcacaggct
     2881 gcgcaagaag ctcgagcccg gcggcgtgcg aatcgtgacc gtgcgcgggc tgggctacag
     2941 ccttgaaaaa tcggactccc gctagaagcc cgctcagcct gctggtcgag atcctcgact
     3001 ggctgctcgc cccgctcatc atcctgtggc cgctgtcggt ggtggtgacg ttctggatcg
     3061 cgaacgcgat cgcgaatatt ccgtacgacc gcgcgctcgc cgattcggtg aacgcgctcg
     3121 cgcgccacgt cagcttcaat gacaagaaag gcggcgcgcg gctggcgctg ccggcagcgg
     3181 cgtcgagctt cctgcgcacc gacgagctcg acacgatcta cttccaggtc cgcggcaccc
     3241 gcggcgaagt gctgggcggc gaccgcgacc tcgccgagat tttccccgag gagggccaga
     3301 ggcagcacat cgtctacttg cgcgacgccg agtggcacgg cgacccggtg cgcgtcgcct
     3361 acatgttcat gttcgacccg caggcgccgc accgccgggt cgtggtgcag gtgggcgaga
     3421 cgctccacaa gcgccagcgg ctttccaacg agatcatcgc cggcatcatc atcccgcagt
     3481 tcttcctctt tccggtggtc gcgctcctcg cctggttcgg gctgcagcgc ggcacgcaac
     3541 cgctcgagcg gctgcgccag cgcctgctcg gccggcgccc gggagacctt tcgccgatcg
     3601 acgtgcgcga ggtgccggag gaggtggcgc cgctcatcga agcgttcaac ggcgtcatgg
     3661 agcggctgtc gagctcgatg cgcgcgcagg agcgcttcat cgcggatgcg gcgcaccaga
     3721 tgcgcacccc gctcgccggg ctgcgcacgc agctcgagtt cgcgctgcgc cagggggacc
     3781 cggccgacct gcgcgcgagc ctcgagcgca tccgctcgag cgtcgagcgc gcgacgcgca
     3841 tgatctcgca gctcctgtcg ctcgcgcgct cgcagagcga gaccccgccc aagttcgagc
     3901 ctgtggacat gaacgcgctc gcgcgcgaag tgacccagga atgggtcgag ccggcgcttg
     3961 ccaagcgcat cgacctcggc ttcgagggct cggtcgagtc cgcggtcgtg gaaggcagcc
     4021 gcctgctgct ccacgagctg ctcaacaatc tcgtcgataa cgcggtgcgc tatacgccgc
//
LOCUS       DASUNT010000153         2173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_546311, whole genome shotgun sequence.
ACCESSION   DASUNT010000153 DASUNT010000000
VERSION     DASUNT010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546311"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VFX94_02995"
     CDS             complement(<1..623)
                     /locus_tag="VFX94_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5767177.1"
                     /translation="MLTLLAFEANAQGLKGSETVILRVEAESIQAHLYKPQGKDRRPA
                     IVLVHGFDAVSESREGFWARELAKLGVVTLVVESFSNPSRMSTAQGVRDAYAALGYLA
                     QQSYVDAARIGIMGMSRGGSVALRAVGKRKHGGGPGFAAAVALYPGCVAQYRNPEPGA
                     PIQVLLGGRDDYSAVKHCSEYLERMRAAGGAVELHVYPEARHGFDGDT"
     gene            complement(635..>2173)
                     /locus_tag="VFX94_03000"
     CDS             complement(635..>2173)
                     /locus_tag="VFX94_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX5767178.1"
                     /translation="EAAIGFLKAKRPIRRIRDMPAKLNGDPEAFFAQVREQVAKESRG
                     YPAPLEIVACAEAAATLPFDQGRKVEREGFNRLVNSAESKALRHMFFAERQTSKIPDV
                     PEDTPVRPIEKAAVIGAGTMGGGIAMCFANAGIPVTVVETTQQALDKGLEKIKANYAA
                     TVAKGRLAQSDMDRRMGLIRGTTNLAQAGDANIVIEAVFERMDVKQDLFRKLDAIVQK
                     GAILATNTSTLDVDRIAEATTRPQELIGTHFFSPANVMRLLEVVRGRKTAKDVLATTM
                     KLGKQLKKLPVVSGVCDGFIGNRMLEKYVQQSLFLLEEGATPAQVDGALQRWGMAMGP
                     FTMYDMAGNDIGWEIRKRRASERPDMVYSGFADRICEKGWFGQKTGRGWYRYEKGSRK
                     PLVDAEVEKLLDDYRKEIGVRPRKISDEEIIERCIFALANEGARILEEGIALRASDID
                     MVYLTGYGFPPYRGGPMFHADEVGLDKVLSSIRKFQAGYQGSQWQVAPLLEKLAKEGK
                     RFNP"
BASE COUNT          317 a          741 c          706 g          409 t
ORIGIN      
        1 gtgtcgccgt cgaagccgtg ccgggcctcg ggatagacat ggagctcgac cgcgccgccg
       61 gcggcgcgca tgcgctcgag gtactcgctg cagtgcttca ccgcgctgta gtcgtcgcgc
      121 ccgccgagca gcacctggat cggggcgccg ggctcggggt tgcggtattg cgccacgcag
      181 cccgggtaga gcgcgaccgc ggcggcgaaa ccggggccgc cgccgtgctt gcgctttccg
      241 accgcgcgca gcgcaaccga gccgccgcgc gacatgccca tgatgccgat gcgcgccgca
      301 tccacgtagg attgctgcgc caggtagccg agcgcggcgt acgcatcgcg cacgccctgc
      361 gcggtgctca tccgtgacgg attgctgaag ctttcaacca ccagggttac cacgccgagc
      421 ttggcgagct cgcgcgccca gaagccttcg cgcgactcgc ttaccgcgtc gaacccgtgc
      481 acgagcacga tcgccgggcg acggtccttg ccttgcggct tgtagagatg cgcctggatc
      541 gattcggcct ccacgcgcag gatgacggtc tcgctgccct tgaggccctg ggcgttcgcc
      601 tcgaacgcga gcagcgtcaa cacaagcagc catctcaggg attgaagcgt ttgccctcct
      661 tcgccagctt ctcgagcagc ggcgcgacct gccactggct gccctggtat ccggcctgga
      721 atttcctgat cgaagaaaga accttgtcca gcccgacctc gtcggcgtgg aacatcggcc
      781 cgccgcggta gggcgggaag ccgtagccgg tgaggtacac catgtcgatg tcggaggcgc
      841 gcagcgcaat gccttcttcg aggatgcgcg cgccctcgtt cgcgagcgcg aaaatgcagc
      901 gctcgatgat ctcctcgtcg gaaatcttcc ggggtctgac cccgatctct ttgcggtagt
      961 cgtcgaggag cttttcaacc tccgcatcga cgagcggctt gcggctgccc ttctcgtagc
     1021 ggtaccagcc gcgcccggtt ttctgaccga accagccttt ttcgcagatg cggtcggcga
     1081 aacccgagta caccatgtcg ggccgttcgc tcgcgcgccg cttgcggatc tcccagccga
     1141 tatcgttgcc cgccatgtcg tacatcgtga acgggcccat cgccatgccc cagcgctgga
     1201 gcgccccgtc gacctgcgcc ggcgtcgcgc cctcttcgag caggaacagg ctctgctgca
     1261 cgtacttctc gagcatgcgg ttgccgatga agccgtcgca caccccggac accacgggca
     1321 gcttcttcag ctgctttccg agcttcatgg tggtggcgag cacgtccttt gccgttttcc
     1381 tgccgcgcac cacctcgagg agccgcatca cgttcgccgg tgagaagaaa tgcgtgccga
     1441 tcagctcctg cggacgcgtg gttgcctcgg cgatccggtc gacgtcgagc gtggaggtgt
     1501 tcgtggcgag gatcgcgccc ttctgcacga tcgcatcgag cttcctgaaa agatcctgct
     1561 tcacgtccat gcgctcgaat accgcctcga tcacgatgtt ggcgtcgccg gcctgagcga
     1621 ggttggtcgt gccgcggatg aggcccatgc gccggtccat gtcggactgc gccaggcggc
     1681 ccttggccac ggtggccgcg tagttggcct tgatcttttc cagtcccttg tcgagcgctt
     1741 gctgcgtggt ctcgactacg gtgacgggaa tgccggcatt ggcgaagcac atcgcgatgc
     1801 cgccccccat ggtgccggcg ccgatgaccg ctgccttctc gatcggccgc accggggtat
     1861 cttcgggcac gtcggggatc ttgctcgtct ggcgctcggc gaagaacatg tggcgcagcg
     1921 ccttcgattc ggcgctattg acgaggcgat tgaagccctc gcgctcgacc ttgcggcctt
     1981 ggtcgaaggg cagcgtggcc gcggcctcgg cgcacgccac gatctcgagc ggcgccgggt
     2041 agccgcgcga ctccttcgcc acctgctcgc gcacctgggc gaagaaggct tcgggatcgc
     2101 cgttgagctt cgcgggcatg tcgcggatcc ggcgaatcgg tcttttcgct ttgaggaaac
     2161 cgatcgcggc ctc
//
LOCUS       DASUNT010000154         2509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_108348, whole genome shotgun sequence.
ACCESSION   DASUNT010000154 DASUNT010000000
VERSION     DASUNT010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108348"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFX94_03005"
     CDS             <1..336
                     /locus_tag="VFX94_03005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5767179.1"
                     /translation="NLVDNAVRYTPPGGRVTARVAVDKSTRDAVTVEIEDNGVGIAEP
                     DRELIFERFYRAAGTETTGTGLGLPIVRSIAAQHRASVQVHPNPVERGSIFTVTFPRL
                     PFVPQREAA"
     gene            380..455
                     /locus_tag="VFX94_03010"
     tRNA            380..455
                     /locus_tag="VFX94_03010"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:413..415,aa:Phe,seq:gaa)
     gene            complement(992..1231)
                     /locus_tag="VFX94_03015"
     CDS             complement(992..1231)
                     /locus_tag="VFX94_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767180.1"
                     /translation="MIVPGMRNRSKGRLGLKVIHRHRAGNSTFRRVSELVFVRGRPRA
                     VLGWIDTGGLRTPIYICELDPAKLRKATALRNTYY"
     gene            1335..2036
                     /locus_tag="VFX94_03020"
     CDS             1335..2036
                     /locus_tag="VFX94_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5767181.1"
                     /translation="MQSRSTLLGLRKVELFKGIDTYSLCEIAAQCRWARCRRNDYVVR
                     RGDMGAEVYFVVDGMLRVTAPAGRGRRITLQDVGAGELFGEHSAIDGRERLADVRALR
                     ESLVASIPAEAFREIVASHGLVRERLLQRLARSVRELTDRLLEHGAQPVQRRIRTELA
                     RLARAAGVDANRARLDPAPTHSEIASRVGTRREQVTRELSRLARQGLLERAGRTLVMR
                     DLAALDVLAGKPGPD"
     gene            2104..>2509
                     /locus_tag="VFX94_03025"
     CDS             2104..>2509
                     /locus_tag="VFX94_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5767182.1"
                     /translation="MIGIMLLDDHALVRQALGRLLNDEPDLRVVGSFDQGAAAVAFAV
                     RERPEVAILDVAMPGASGIEVAVELRAASPQTRLLMLSMHDRADYVEQALRAGADGYV
                     LKECAGAELVAAVRAVHAGRRYLSARLERSRGE"
BASE COUNT          384 a          814 c          885 g          426 t
ORIGIN      
        1 aacctggtgg acaacgccgt gcgctacacc ccgcccggcg gccgggtgac cgcgcgcgtc
       61 gcggtggaca aatcgacgcg cgatgccgtg accgtcgaaa tcgaggacaa cggcgtcggc
      121 atcgccgagc ccgatcgcga gctgatcttc gagcgcttct accgcgccgc cggcaccgag
      181 acgacaggca ccgggctcgg gctcccgatc gtgcgctcca tcgccgccca gcaccgcgcc
      241 tcggtgcagg tgcacccgaa cccggtcgag cgcggcagca tcttcacggt gacctttccc
      301 cgcctgcctt tcgttccgca gcgcgaagcc gcgtagctgc ccttgctata attcgcgcct
      361 ttccgacgca ccccccggcg gccaagtagc tcagttggta gagcagagga ctgaaaatcc
      421 ttgtgtcggt ggttcgattc cgcccttggc caccaacata atcaacgtct cgcttcgtac
      481 cgcgtgcacg agtggagttt tcgctgctat cgctcgcgca cgccttccgc ctcgtgggat
      541 gctttgcacg caatgcagac ttgaaacgct aggggccgga gtgcaccgtc aataggtcgg
      601 cccagcccga cgttggtatt tcatcgattt ggcgcgctag cggccttccg acgcgcgtcc
      661 gcaggcgaac ggtgtataag aaagcgatga ataaaccaat aggtcattgc aacatcgtct
      721 atgtaagacg cgggcaggcc actggctgga gatggcaggc agtatcgccc gcaggagagg
      781 cgaagccgtc cgccgaggtc tacgctctgt tctacgattg cgtcgtggct gcacgagcgc
      841 gcgggtatac gcccgttacg ccgctgaagt gctcgtgagc agtcgctgga acacggatcc
      901 atcggctccc gttaggcgct gcgcgctact acattttgct ttcccgcgcg ggtagcgtcg
      961 gctcgtagcg cgggtcgacg gtttcctcgt cctagtagta agtgttcctg agggcggtgg
     1021 ccttgcgcag cttggccggg tcgagctcgc agatgtagat cggcgtgcgc aggcccccgg
     1081 tgtcgatcca gccgagcacc gcgcgggggc ggccgcgcac gaacaccagc tcggagaccc
     1141 gtcgaaacgt cgagttgccg gcgcggtgac ggtgaatcac tttgaggcca aggcggcctt
     1201 tgctgcggtt gcgcataccg ggaacaatca cccatgcagg cttcgtgccg ctccacgaag
     1261 gggcgacaag cgatcgcctg gcggcgggag attgttttat agtctggcct gggggggagg
     1321 gtcggatggc cgctgtgcag tcgaggtcga cgctgctcgg cctgcgaaag gtcgagctct
     1381 tcaagggtat cgatacctac agcctgtgcg agatcgcggc gcaatgccga tgggcacgct
     1441 gcaggcgcaa cgactacgtc gtacgccgcg gcgacatggg cgccgaggtc tatttcgtgg
     1501 tcgacgggat gctgcgggtc accgcgccgg ccgggcgcgg ccggcgcatc actttgcagg
     1561 acgtgggcgc cggcgagctc ttcggcgagc attccgcgat cgacggccga gagcggctgg
     1621 ccgacgtgcg cgcgttgcgg gaatcgcttg ttgcctcgat cccggccgag gctttccggg
     1681 aaatcgtggc aagccacggc ctggtgcggg agcggctgct gcaacgcctc gcgcgctcgg
     1741 tgcgcgagct gaccgaccgg ctgctcgagc acggcgccca gccggtgcag cggcgcatcc
     1801 ggacggagct ggcgcggctc gcgcgcgcgg cgggcgttga tgcgaaccgc gcccggctgg
     1861 atcccgcgcc cacgcactcg gagatcgcga gccgcgtcgg taccaggcgc gagcaggtca
     1921 cgcgtgagct ctcccgactg gcccggcagg ggttgctcga gcgagccggc aggacgctcg
     1981 tcatgcggga cctggccgcg ctcgacgtgc tcgccggaaa gcccggcccc gactgacaaa
     2041 tcttttccgt gtcctgacag gcctgaaaca agcccgcggg cgcgtcgcct tatcgtgtcc
     2101 gccatgatcg gcatcatgtt gctcgacgac catgccctgg tgcggcaggc gctcggccgg
     2161 ctgctcaatg acgagcctga cctgcgcgtg gtgggctcgt tcgaccaggg tgccgccgcg
     2221 gtggcgttcg ccgtgcggga aaggcccgag gtcgcgatcc tcgacgtcgc gatgccgggg
     2281 gcgagcggaa tcgaggtggc ggtcgagctg cgcgccgctt ccccgcagac gcggctgctg
     2341 atgctgtcca tgcatgaccg cgccgactat gtggagcagg cgctgcgcgc gggcgcggac
     2401 gggtacgtgc tgaaggaatg tgcgggcgca gagctggttg cggccgtgcg cgccgtgcac
     2461 gcgggccggc gctatctgag cgcccggctg gagcgctcgc gcggcgagg
//
LOCUS       DASUNT010000155         2623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_284018, whole genome shotgun sequence.
ACCESSION   DASUNT010000155 DASUNT010000000
VERSION     DASUNT010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284018"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VFX94_03030"
     CDS             complement(<1..905)
                     /locus_tag="VFX94_03030"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HEX5767183.1"
                     /translation="MRLNGILLAGLCALGPALVHAQPADALAWLRKIQDATQKLSYRG
                     TFVYQNGGRSETSSITRYVDASGDIEKLEVMDGVPREIVRTKDTVRCYLPGKSLVKVD
                     RRTERDFPALLPERITALARHYEIELGGVQRIAGYDCQAIVLRPKDNLRYGYRLYADT
                     GSGMMLRAVTLDPAGEQVEQFSFTQLTIGGVTRDMVKPRHASAKWRIEDAEAAPARLA
                     GWGLSAELPGFQKVIELKRKMGESRPVGQVVYSDGLAAVSVFIEPLENRQVQTGLASM
                     GAIHIYTREVANHVVTVVGDAPAVSV"
     gene            complement(902..1408)
                     /locus_tag="VFX94_03035"
     CDS             complement(902..1408)
                     /locus_tag="VFX94_03035"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HEX5767184.1"
                     /translation="MKERLSALMDGELDDKSAAEVMNALGSERDALRTWRTYQLISDA
                     MRESRLLSADFASRLAERLAAEPTVLAPRALQTQSRKWYALSAAASLAAVALVGWVAF
                     APPQPPTPAPTPVAQTPTEPKPNIVPLPTTANDYLLAHQAFSPRMYLQGMAPYARTVA
                     EQAVEPRR"
     gene            complement(1433..2032)
                     /gene="rpoE"
                     /locus_tag="VFX94_03040"
     CDS             complement(1433..2032)
                     /gene="rpoE"
                     /locus_tag="VFX94_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HEX5767185.1"
                     /translation="MSDRDIDRQLVARAQRGDKQAFELLVEKYQRKLARLLSRFIRDP
                     AEVEDVTQEAFIKAYRALPAFRGDSAFYTWLYRIGINTAKNYLMAMGRRAPTSTEVEA
                     EDAEGFEEGEQLRDINTPESVLLSNEIARTVNATIEQLPEELRTAIQMREIEGMSYED
                     IAKAMDCPIGTVRSRIFRAREAIAEQLRPLLDTRKDRRW"
     gene            complement(2180..>2623)
                     /locus_tag="VFX94_03045"
     CDS             complement(2180..>2623)
                     /locus_tag="VFX94_03045"
                     /inference="COORDINATES: protein motif:HMM:NF018908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HEX5767186.1"
                     /translation="AALSGTRLELARSWLLAAAIFLLHGAGAASILTFLQGWTGAALS
                     AGMLALGTAAAWARALHGSPASVRAIELSGGVMTLELNDGRRCTVELAASRHVSRFVV
                     TLPVRRPMRRTVFVTRDMLHAEEFRRLRLWALWGKLPVAAKQLPA"
BASE COUNT          390 a          952 c          840 g          441 t
ORIGIN      
        1 acgcttaccg cgggggcatc ccccacgacc gtcacgacgt ggttcgccac ctcgcgcgta
       61 tagatatgga ttgcgcccat gctggcaagc cccgtctgca cctgacggtt ctccagcggc
      121 tcgatgaaca ccgagaccgc cgccagccca tcggagtaca ccacctgccc cacgggccgg
      181 gactcgccca tcttgcgctt gagctcgatc accttctgga acccgggcag ctcggcggac
      241 agcccccagc ccgcgaggcg ggccggcgcg gcctcggcgt cctcgatgcg ccacttggcc
      301 gaagcgtgcc gcggcttcac catgtcgcgc gttacgccgc cgatggtgag ctgcgtgaaa
      361 gaaaactgct cgacttgctc gccggccgga tcgagcgtca ccgcgcgcag catcatgccg
      421 ctgccggtgt cggcgtagag ccggtagcca tagcgcaggt tgtccttggg ccgcagcacg
      481 atcgcctggc agtcgtagcc cgcgatgcgc tgcaccccgc ccagctcgat ctcgtagtgc
      541 cgcgcgagcg ccgtaatgcg ctcggggagg agcgccggga aatcgcgctc ggtgcgccgg
      601 tcgaccttca ccaggctctt gccgggtagg tagcagcgca ccgtgtcctt cgtgcgcacg
      661 atctcgcgcg gcacgccgtc catgacttcg agcttctcga tgtcgcccga ggcgtccacg
      721 tagcgcgtga tgctggaggt ctcgctgcgg cctccgttct ggtagacgaa ggtgccgcgg
      781 taggacagct tctgcgtcgc gtcctggatc ttgcgcagcc acgcgagcgc gtcggccggc
      841 tgcgcatgca ccagcgcggg acccagcgcg cacaggccgg cgagcaggat cccgttcagc
      901 ctcaccggcg cggctccacc gcctgctcgg ccaccgtgcg cgcgtagggc gccatgccct
      961 gcaggtacat gcgcggcgag aacgcctggt gcgcgagcag atagtcgttc gccgtagtgg
     1021 gtagcggaac gatattgggc tttggctcgg tcggtgtctg cgccaccggc gtgggggccg
     1081 gggtgggcgg ctgcggcggc gcaaacgcca cccagccgac gagcgcaacg gcagcgaggc
     1141 tcgcggccgc cgacagcgcg taccatttgc gagattgcgt ctgcagcgca cgcggcgcga
     1201 gcaccgtcgg ctcggccgcg agccgttcgg ccaggcgcga agcgaagtcg gccgagagca
     1261 gccggctctc gcgcatcgca tcgctgatca gctggtacgt gcgccacgtg cgcagggcgt
     1321 cgcgctcgct tccgagcgcg ttcatcacct cggcggcgga cttgtcgtcc agctcgccgt
     1381 ccatcaatgc cgagagcctt tccttcatac ccctaccacc tgccttttcg cgttaccacc
     1441 tgcggtcttt ccgggtgtcc aggagcggcc gcagctgctc ggcgatcgct tcccgcgccc
     1501 ggaagatcct cgatcgaacc gttccgatcg ggcagtccat cgctttcgcg atgtcctcgt
     1561 aactcatccc ctcaatctcc cgcatctgaa tcgcggtgcg cagctcttcg ggcagctgct
     1621 ctatcgtcgc attcaccgtt cttgcgatct cgttcgacag cagcacgctc tcgggcgtgt
     1681 tgatgtcgcg cagctgctcg ccttcctcga atccctccgc gtcctcggcc tctacctccg
     1741 tcgaggtcgg cgcccgccga cccatggcca tgagatagtt cttggccgta ttaattccaa
     1801 tccggtacag ccacgtgtaa aacgcgctgt cgccgcggaa tgcagggagg gcccggtagg
     1861 ccttgatgaa cgcctcctgc gtcacgtcct cgacctctgc ggggtcgcgg atgaagcgcg
     1921 acagcaggcg cgccagcttg cgctggtact tctcgacaag cagctcgaaa gcctgcttgt
     1981 cgccgcgctg cgcccgtgcc accagctggc gatcgatgtc gcggtcgctc atcgaccgcc
     2041 ctcgactgcc ggctcccggc gccgcgcagg ggctccccac cccgtacaaa cagcccgtgg
     2101 acttccgtcc cggttccaac gcctatgtgc cacagacaca cgcacttgga aatagttcgt
     2161 gccgttcccg gggtttgcct caggccggca gttgcttcgc cgccacgggc agctttcccc
     2221 agagcgccca gaggcgcagc cgcctgaact cctcggcgtg aagcatgtcg cgcgtcacga
     2281 agacggtgcg ccgcatcggg cggcgcaccg gcagcgtcac cacgaagcgg ctgacgtggc
     2341 ggctcgctgc gagctccact gtgcagcgcc tgccgtcgtt caactcgagc gtcattacgc
     2401 cgcctgacag ttcgatcgcc cgcaccgacg ccggcgaccc gtgtagcgcg cgcgcccagg
     2461 cggcggcggt gccaagcgcg agcatcccgg cgctcagcgc ggcgccggtc caaccttgca
     2521 gaaatgtcaa tatcgacgcc gcgccagcac cgtgcagcaa gaagatcgcg gccgccagca
     2581 gccaggagcg ggcgagctca agccgcgtgc cgctcaaggc cgc
//
LOCUS       DASUNT010000156         2567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_20470, whole genome shotgun sequence.
ACCESSION   DASUNT010000156 DASUNT010000000
VERSION     DASUNT010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20470"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VFX94_03050"
     CDS             <1..215
                     /locus_tag="VFX94_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767187.1"
                     /translation="YVFESAYEIVLRNAKKEAASVVVREPVPGDWTMLEETQRHAKVA
                     AGTAEWQVRVPAEGSTTVRYRVLVRY"
     gene            complement(234..839)
                     /locus_tag="VFX94_03055"
     CDS             complement(234..839)
                     /locus_tag="VFX94_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 domain-containing protein"
                     /protein_id="HEX5767188.1"
                     /translation="MISQLVIAGWTGRDEAALRKHIKELEEIGVKPPKTTPIFYRVSA
                     NLFCHAEEIQVSGPQTSGEVEFVLISSAEGLKVAVGSDHTDRKAETIGVSLSKQLCAK
                     PVSRESWRYDEVKPQWEKLLLRAWADGELYQEGPVSAMRSPEDLMARYPLKPGWAMFG
                     GTLAAKGGIRPAGRFAMELEDPVLGRKLRHEYRIHVLPVEG"
     gene            complement(836..2305)
                     /locus_tag="VFX94_03060"
     CDS             complement(836..2305)
                     /locus_tag="VFX94_03060"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5767189.1"
                     /translation="MLKTGNEHLESLRDGRVVYIGSERVADVTTHPAFRNAARTVASI
                     YDMKIAEREAMSFEEGGERYSIYFLQPRTRDELARRTRGHRLIADLTYGMFGRSPDHV
                     ASFVTGMAMKPDELKAPCGRAENLLSFYRDIRSSDAYVVYAVLPPQAARNPEFYQRQN
                     IPIPTLSVVREEDDGLVISGMKMLATGAVFANEVWIGNVLPLAPDQKKQAITCSLPIA
                     TKGLSLWSRKPFEREAKSEFDAPLAWRFDETDSMVLCDEVKVPWERVFVHDDALLSRD
                     VYVKTPSHCFGNHQSNVRYWSKMRLLLGVCSKIAHATGAIEVPAVKEVLGHMSAVESM
                     IGGMVNGQIEAFERWPEGYVCFNRRMMYAALEWCTQNYCHFVERLRELCGGGVFQMPA
                     DISVVENEELARKFTTYFQTPQSGAVERMKLFKLAWDMVGSEFAGRHLQYEKFYAGAS
                     FIVRNHAFRETPWNDFHKIVDDLMASYDVPQSRVEKRVA"
     gene            complement(2286..>2567)
                     /locus_tag="VFX94_03065"
     CDS             complement(2286..>2567)
                     /locus_tag="VFX94_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEX5767190.1"
                     /translation="SDLDEQSKKGSRTRLLKIEPGAFSTEPFVHDHWEEVYVVQGDLV
                     VGNDKQGNGGTQCFAPTYACRPPGVYHGPFTSRGGCILHEIHYYAQDRK"
BASE COUNT          425 a          847 c          833 g          462 t
ORIGIN      
        1 gctacgtgtt cgagagcgcc tacgagatcg tgctgcgcaa cgcgaagaaa gaggcggcga
       61 gcgtcgtggt gcgcgagccg gtgccgggcg actggacgat gctcgaggaa acccagcgcc
      121 acgccaaggt cgcggccggc accgccgagt ggcaggtccg cgtgccggcc gagggaagca
      181 cgaccgtgcg ctaccgcgta ctcgtgcgct actgagaggt ccggttctgc gctttaacct
      241 tccaccggaa gaacatggat gcggtactcg tgccgcagct ttcgcccgag caccgggtcc
      301 tcgagctcca ttgcgaatcg cccggcggga cggatgccgc ccttggcggc gagcgtgccc
      361 ccgaacatcg cccacccggg cttcaacgga tagcgcgcca tgaggtcctc gggagagcgc
      421 atggcgctca ccgggccttc ctggtagagc tcgccgtcgg cccaggcgcg cagcaagagc
      481 ttttcccact gcggcttcac ttcgtcgtag cgccacgact cccgcgacac tggcttggcg
      541 cagagctgct tggacagcga caccccgatc gtctccgcct tgcggtcggt gtggtccgac
      601 ccgacggcga ctttcagccc ttcggcgctc gagataagga caaattccac ttcgccgctg
      661 gtctgcggcc cggaaacctg gatttcctca gcgtggcaga agaggtttgc cgacacccgg
      721 tagaagatcg gcgtggtctt cggcggcttg acgccgatct cctcgagctc cttgatgtgc
      781 ttcctgagcg ctgcctcgtc acgcccggtc cagcccgcga tgacaagctg ggagatcacg
      841 caaccctctt ttcgacccgg gattgtggga catcgtagga cgccatgagg tcatcgacga
      901 tcttatggaa gtcgttccac ggcgtctcgc ggaaagcatg gttgcggacg atgaacgagg
      961 cgccggcgta gaacttctcg tattgcaagt gccgcccggc gaactcggag ccgaccatgt
     1021 cccaggcgag cttgaacagc ttcatgcgct cgaccgcgcc gctctgcggg gtctggaagt
     1081 aggtggtgaa cttgcgcgcg agctcctcgt tctcgacgac cgagatgtcg gcaggcatct
     1141 ggaacacgcc gccgccgcac agctcgcgca gccgctcgac gaaatggcag tagttctgcg
     1201 tgcaccactc gagcgccgca tacatcatgc gccggttgaa gcaaacgtag ccctcgggcc
     1261 agcgctcgaa cgcctcgatc tggccgttca ccatgccgcc gatcatgctc tccaccgcgg
     1321 acatgtgccc gagcacttcc ttgaccgccg gcacctcgat cgcgcccgtg gcatgcgcga
     1381 tcttgctgca caccccgagc agcaggcgca tcttggacca gtagcgcacg ttcgactggt
     1441 ggttgccgaa gcagtggctc ggcgtcttca cgtagacgtc gcgagaaagc agcgcgtcgt
     1501 cgtgcacgaa cacgcgctcc cacggcacct tgacctcgtc gcacagcacc atgctgtcgg
     1561 tctcgtcgaa gcgccaggcg agcggtgcgt cgaactcgga cttcgcctcg cgctcgaagg
     1621 gcttgcgcga ccagagcgag agtcccttgg tcgcgatcgg cagcgagcag gtgatcgcct
     1681 gcttcttctg gtccggcgcg agcggcagca cgttgccgat ccagacctcg ttcgcgaaca
     1741 ccgcgccggt ggcgagcatt ttcatgcccg agatcaccag cccgtcgtcc tcctcgcgca
     1801 ctaccgagag cgtcgggatg ggaatgttct gccgctggta gaactcgggg ttgcgcgccg
     1861 cctgcggcgg cagcacggca tagaccacat aggcatcgct gcttcggatg tctcgataga
     1921 aactcaggag attttcggcg cgcccgcacg gcgccttcag ctcgtcgggc ttcatcgcca
     1981 tcccggtgac gaagctcgcg acgtggtcgg gggagcggcc gaacatgccg taggtgaggt
     2041 ccgcgatcag ccggtggccg cgcgtgcgcc gcgcgagctc atcgcgcgtt ctcggctgca
     2101 ggaagtagat cgagtagcgc tcgccgcctt cctcgaagct catcgcctcg cgctcggcga
     2161 tcttcatgtc gtagatcgag gcgacggtgc gcgccgcgtt cctgaacgcc gggtgcgtgg
     2221 tgacgtcggc aacacgctcg gagccgatgt agaccacccg gccgtcgcgc agcgactcta
     2281 gatgttcatt tccggtcttg agcatagtag tggatctcgt ggaggatgca gccgccgcgg
     2341 gaagtgaacg gaccatggta gacgcccggc gggcggcacg cgtaggtcgg cgcaaagcac
     2401 tgggtgccgc cgtttccctg cttgtcgttg ccgacgacga ggtctccctg cacgacgtag
     2461 acctcttccc agtggtcatg gacgaaaggc tcggtcgaga acgcgcccgg ctcgatcttg
     2521 agcagccggg tgcggctgcc tttcttgctc tgctcgtcca ggtcgga
//
LOCUS       DASUNT010000157         2923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1160954, whole genome shotgun sequence.
ACCESSION   DASUNT010000157 DASUNT010000000
VERSION     DASUNT010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160954"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..394
                     /locus_tag="VFX94_03070"
     CDS             179..394
                     /locus_tag="VFX94_03070"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX5767191.1"
                     /translation="MRSRPARNEKPLHWVGSAKRDLLGFPEKVVDNIGYALGVVQYGG
                     HPRSAKPWKGFGPGLSEIVEDDASGTY"
     gene            440..775
                     /locus_tag="VFX94_03075"
     CDS             440..775
                     /locus_tag="VFX94_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5767192.1"
                     /translation="MARKRTEVEAGSGNVFADLGYADARSRSLKVELAMEVNRILGEL
                     GLAQDRAAKLLGIRQPHVSDLVRYRLNRFSVERLMDFLTRLGRDVEIRIAPRRSGRRR
                     SAVQVRQLE"
     gene            843..1802
                     /locus_tag="VFX94_03080"
     CDS             843..1802
                     /locus_tag="VFX94_03080"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HEX5767193.1"
                     /translation="MQFISESLAALRLGAPHAHLNLALFPLIADFDRAPDYLLLDDAL
                     ERKLACVSEVSAEGRVPELAFENSSSEKILLVDGDELVGARQNRILNLTILVGGAQKL
                     VIPVSCVEQGRWRYRRGGAPSDFASAGRALFARARAHKAAGVTASLRSHGERHANQSQ
                     VWADVADKVASCGAASETLAMADAYESRAAQVEAYVRAFRAAPRQHGAVMTIDGQPVG
                     LELFDSAAAFSRYLPKLVRSYALDAIETADGLISASGLAPSEAQVRSFLDRVSAAAGE
                     RFPALGEGEDIRLTGSAVAGGALAVDGRLVHLAGFAVGSGPRA"
     gene            1845..2126
                     /locus_tag="VFX94_03085"
     CDS             1845..2126
                     /locus_tag="VFX94_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767194.1"
                     /translation="MDCILTPHARVRMQQRGIRAEMLDALLDFGREIHVAGGRDLVFF
                     DKRARARLAKTGLVSGAQADRLCKSYAVLSSDGIVITVGHRYRRIPRGR"
     gene            2242..>2923
                     /locus_tag="VFX94_03090"
     CDS             2242..>2923
                     /locus_tag="VFX94_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767195.1"
                     /translation="MRFSARQPEMTASMPIERHPAVEAPRLFTPTQLSAHLACAHYTQ
                     LERKRRAGELDVEFTPDPRLEALWTRGAEHERAYVERLRQAGSSIVDLRERRDPQATL
                     AAMREGAQVIVQAPLGNEHFFGIADVLLRVGKSYEPVDTKLARETRAGTILQLCTYCE
                     MLAAMQGELPLRFHVVTPLKEEAYRTADFAAYFRLIRSRFMTAVTPAQEGVTPARAGA
                     PFPETYPDP"
BASE COUNT          457 a         1004 c          970 g          492 t
ORIGIN      
        1 gggtcagagc ccctggggtc agagcccgaa ttaacacagc tacgcgttaa taggtttggg
       61 gctctgaccc cgaatttgat gtggggctct gaccccaaac tttctgcgtc accgcggcgc
      121 tcgccggaac gttgatcggg gaacgcctcc ggtcccatac cgctttcggt ataatcccat
      181 gcgcagccgt cctgcccgga acgaaaagcc gctccattgg gtcggctcgg cgaagcggga
      241 cctgctcggg tttccggaaa aggtcgtgga caacatcgga tatgccttgg gcgtggtgca
      301 atacggcggg catccgcggt cggcgaagcc ctggaagggt ttcggtcccg gcttgtcgga
      361 gatcgtcgag gacgacgcgt ccggcacgta ctgatcgagg agcggctgaa ggcggcgaag
      421 cgggactacg aggcgcgcta tggcaaggaa gcggactgag gtcgaagcgg ggagcggcaa
      481 cgtatttgcc gacctcggct atgcggatgc gcgcagccgc agcctcaagg tcgagctcgc
      541 catggaggtg aaccgcatcc tcggtgagct cgggctcgcg caggaccgcg cggcgaagct
      601 gctcggcatc cgccagccgc acgtctccga cctggtccgc taccgtctca accgtttctc
      661 ggtcgagcgg ctcatggact tcctgacccg cctgggcagg gacgtcgaga tccgcatcgc
      721 gccgcgtcgt tccgggcggc ggcgttccgc cgtgcaggtt cgtcagctgg agtagggcgg
      781 ctcgggctca ctgcctgagc cactacccgc cgcacactgc tgcctcctat acggaggtag
      841 tcatgcagtt catctcggaa tccctcgccg cgctccgcct cggcgcccct cacgcccacc
      901 tgaacctcgc gctcttcccg ctcatcgcgg acttcgaccg cgcgccggac tacctcctgc
      961 tcgacgacgc gctggagcgc aagctcgcgt gcgtgagcga ggtctcggcc gaaggccggg
     1021 tgcccgagct cgcgttcgag aactcttcca gcgagaagat cctcctggtc gatggcgacg
     1081 agctcgtcgg tgcaagacaa aaccgcatcc tcaacctcac gatcctggtt ggcggcgctc
     1141 agaagctcgt gatcccggtc tcctgcgtgg agcaggggcg ctggcgctac cggcgcggcg
     1201 gcgccccctc cgacttcgcg agcgccgggc gcgcgctatt cgccagggcg agagcgcaca
     1261 aggcggccgg cgtgaccgca tcgcttcgca gccacggcga gcgccacgcc aaccagtcgc
     1321 aggtctgggc cgacgtcgcc gacaaggtcg cctcctgcgg cgccgcctcc gagaccctcg
     1381 cgatggccga cgcctacgag agccgcgccg cgcaggtcga ggcctacgtg cgcgccttcc
     1441 gcgcggcgcc gcggcagcac ggcgccgtaa tgacaatcga cggccagccg gtggggcttg
     1501 agcttttcga ctcggccgcg gccttttcgc gttacctccc gaagctcgta cgcagctacg
     1561 cgctggacgc catcgagact gccgacggcc tcatttctgc ctctgggctt gcgccttccg
     1621 aggcgcaggt ccgcagcttc ctcgaccgcg tctcggccgc cgccggcgag cgcttcccgg
     1681 cgctcggcga gggcgaggac attcgcctca ccggctcggc tgtcgcgggt ggcgcgctgg
     1741 cagtggacgg gcgcctggtt cacctggccg gcttcgcagt ggggtcaggt ccgcgagctt
     1801 gagaaccgaa gcgtccccac gatttcggac ccgacccgaa tttcatggac tgcatcctta
     1861 ccccgcatgc gcgtgtgcgg atgcagcagc ggggcatccg cgccgaaatg ctcgatgcgc
     1921 tcctcgactt cggcagggag atccacgtcg ccggcggccg ggatctcgtg tttttcgaca
     1981 agcgggcacg cgcacggctt gccaaaaccg ggctcgtaag cggcgcgcag gcggaccggc
     2041 tttgcaagag ctacgcggtg ctgagctcgg acgggatcgt gatcacggtc ggtcaccgct
     2101 accgtcgcat cccgcgcggg cgttgaattc ttgaggtttt gttgacgcga ggcgctttcg
     2161 gcatctgaat ctgttgagtc ccgacaggga ttcggggtat agagaagccg gggacgataa
     2221 tttgaaaaag catacgggga ggtgcgcttt tcggcgcgcc agcccgagat gaccgcgtcg
     2281 atgccgattg agcgccatcc ggccgtagag gcgccaaggc tgttcacgcc cacgcagctt
     2341 tccgcgcacc tcgcctgcgc gcattacacg cagctcgagc gcaagcgtcg agcaggcgag
     2401 ctcgacgtcg agttcacgcc cgatccgcgg ctcgaagcgt tgtggacccg cggtgccgag
     2461 cacgagcgcg cctacgtcga gcgccttcgc caggctggct cgagcatcgt cgacttgcgg
     2521 gagagaaggg acccgcaggc cacgcttgct gccatgcgcg agggcgcgca ggtcatcgtg
     2581 caggcgccgc tcggcaacga gcatttcttc ggcatcgccg atgtgctcct tcgagtgggc
     2641 aagtcctacg agccggtcga caccaagctc gcgcgcgaga cacgcgcggg caccatcctc
     2701 cagctctgca cctactgcga gatgctcgcc gccatgcagg gcgagctgcc tttgcgcttc
     2761 cacgtcgtca cgccgctgaa ggaggaggcc taccgcaccg ccgacttcgc cgcctacttc
     2821 cggctgatcc gctcccggtt tatgaccgcc gtcacccccg cgcaagaggg cgtcaccccc
     2881 gcgcgggcgg gggccccatt tcctgaaact tatccggacc ccg
//
LOCUS       DASUNT010000158         4888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1073161, whole genome shotgun sequence.
ACCESSION   DASUNT010000158 DASUNT010000000
VERSION     DASUNT010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073161"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="VFX94_03095"
     CDS             <1..1151
                     /locus_tag="VFX94_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5767196.1"
                     /translation="RNGFAIPDLAGPLVQQKTTQYVARPEYLQRILSRSKLYLYHIVE
                     EIEKRNLPTELALLPMVESAFNPMAYSRAHASGLWQFIPGTGRRFELEQNSWYDGRRD
                     IVDSTSAALDYLTKLYEMHGDWHLALASYNWGENAVKKAVTRNRAQGKKTDYASLRMP
                     KETRHYIPKLQALKNIIADPVAYGVDLDAIPNQPYFTVISDTPDIDVHLAARLAEMPV
                     EDFIALNPGFSRPLIRSSSVGRIILPNDNVVAFYENLDTLDGDALVSWQIYHPKQGEK
                     IEAIAKRFDIGVNELRRVNGISPRSTRLPKDLVVPKTREAGDTVAQLPLMYAPPIPQQ
                     GPARAVHVVKRGDTLSSVAKRYRVSVASLKSWNKIGQYLQVGQKLYIR"
     gene            complement(1173..2750)
                     /locus_tag="VFX94_03100"
     CDS             complement(1173..2750)
                     /locus_tag="VFX94_03100"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX5767197.1"
                     /translation="MSLLEVKDLCIDFGPRRVVHNASFTLDAREKLALVGESASGKTV
                     TALSTLRLIENARLSGRIVFNGKNVLAMSADELHQLRGRDIAVIFQEPMTALNPIYTV
                     GDQIVESLELHTNLRGAAAWKEAVQAMRRVGIDEPERRAHSYPHQLSGGQRQRAMIAM
                     ALACSPKLLIADEPTTALDVTIRLQILELLEKLRAEAGMALLLITHDLNLVRRFADRV
                     AVMEKGVLVEQGPTAQVIGRPQHPYTRKLVNSRPARDIAPASAGVLVEASKVRVNYPT
                     RLPGIRGWFKSGNFTAVASVDFDLAPGETLGVIGESGSGKTTLALAVLGLMASHGDIR
                     IGNRSWQQRAERRTMRKDIQVVFQDPLSSLSPRRTVEQIIGEGLEIHEPGLDAAARRG
                     RVLQALYDVGLTEGGRAEPLLGRYPHEFSGGQRQRIAIARALIVKPKVVVLDEPTSAL
                     DVTIQKQVLELLSSLQKKYGLSYILVTHDIDVVRAMAHRVMVMKDSRVVESGPLEQVL
                     QRSKSDYTRRLVEAGYA"
     gene            complement(2791..3873)
                     /locus_tag="VFX94_03105"
     CDS             complement(2791..3873)
                     /locus_tag="VFX94_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5767198.1"
                     /translation="MSSPAVAIDFPVSPAVHASPGRRAWRRFRANRLGYTSLVVFAVL
                     FGVSLLAEVLSNDKPLVARYEGRWYLPILQTLPETTFGGDFSTPTDYLDPLIQQNLSK
                     GANIAVFPLNRYHHSTINYFASEPSPAPPSGENRLGTDDRGRDLLARLLYGFRLSVVF
                     ALIVTAIVSLVGTLYGAIQGYFAGWTDIAMERFNEIWSAMPTLYMLIIFSSLFSASFG
                     LLVILLSVFGWLGIAAYVRAEFLRNRTLDYVRAARALGQTTWKIIRRHILPNSLTPVV
                     TLIPFEMAAAIGALTSLDFLGLGVPPGTPSLGELLNQGKNNLDAWWISLSTFAVLVVT
                     LLLLILIGDALRDALDPRKVLKEDEA"
     gene            complement(3877..4887)
                     /locus_tag="VFX94_03110"
     CDS             complement(3877..4887)
                     /locus_tag="VFX94_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5767199.1"
                     /translation="MIPTLFGILLITFIVIQFVPGGPVEQMVAQFQGFEAGGEGPAAA
                     EGGYRGRQGVDAVRVEEIRQLYGFDKPAPERFAQMIGQFARFDLGKSFFHNKAVGELI
                     LEKLPVSISLGVWTFLLVYLISVPLGIAKAVRAGTPFDTVTTFVVLLGYAIPSFVMGV
                     VLLVFFAGGSFLQWFPLRGITSINWGDMNPIDKVLDYFWHIALPVTAMVLGGFAVITI
                     LTKNSVLEEIRKHYVLTARAKGVDERRVLWRHVFRNAMIPIMTGFPAAFVGAFFSGSL
                     LIETLFSLDGLGLLSYESVIRRDYPVVFGSLFVFTIIGLLTTLARDLSYLWVDPRVKF
                     FD"
BASE COUNT          934 a         1622 c         1569 g          763 t
ORIGIN      
        1 tccgcaacgg gttcgcgatt cccgacctcg ccgggccgct cgtccagcaa aagaccacgc
       61 agtacgttgc gcggcccgaa tacctgcagc gcatcctctc gcgcagcaag ctctacctct
      121 accacatcgt cgaggagatc gagaagcgca atctcccgac cgagctggcg ctgctgccga
      181 tggtggagag cgcgttcaac ccgatggcct actcccgcgc gcacgcttcg ggactgtggc
      241 agttcatccc cggcaccggc cggcgcttcg agctggagca gaactcctgg tacgacggcc
      301 ggcgcgacat cgtcgattcc accagcgccg cgctcgacta cctcaccaag ctctacgaga
      361 tgcacggcga ctggcacctc gcgctcgctt cgtacaactg gggggagaac gccgtcaaga
      421 aggccgtcac gcgcaacagg gcgcagggca agaagaccga ttacgcgagc ctgcgcatgc
      481 cgaaggagac gcggcactac atcccgaagc tgcaggcgct gaaaaacatc atcgccgacc
      541 ccgtggcata cggcgtcgac ctcgatgcga tcccgaacca gccctacttc accgtcatca
      601 gcgacacgcc ggacatcgac gtccatctcg ccgcccggct cgccgagatg ccggtcgagg
      661 acttcatcgc gctcaacccc ggcttcagcc ggccgctcat ccgctcctcg agcgtcggac
      721 gcatcatcct gcccaacgac aacgtcgtgg cgttctacga aaacctcgac accctcgatg
      781 gagacgccct cgtgtcgtgg cagatctatc acccgaagca gggcgagaag atcgaagcga
      841 tcgccaagcg cttcgacatc ggcgtgaacg agctcaggcg cgtcaacggc atttccccgc
      901 gctccacccg cctgccgaag gacctcgtcg tgccgaagac gcgcgaagcg ggcgataccg
      961 tagcccagct tccgctcatg tacgccccgc ccatcccgca gcagggtcct gcgcgcgcgg
     1021 tgcatgtcgt aaagcgcggc gacaccctga gctcggtcgc gaagcgctac cgcgtgagcg
     1081 tggcaagcct gaaaagctgg aacaagatcg gccagtacct gcaggtcggt cagaaactct
     1141 acatccgcta gaaaaagggg tcaggacccc tttcaggcgt agccggcttc gaccagccgg
     1201 cgcgtgtagt cggacttcga gcgctgcagc acctgctcga gcggcccgct ctcgacgacc
     1261 cggctgtcct tcatcaccat cacgcggtgc gccatggcgc gcacgacgtc gatgtcgtgg
     1321 gtgacgagga tgtagctcaa gccatatttc ttctgcagag aagagagaag ctcgagcacc
     1381 tgcttctgga tggtgacgtc gagcgcgctc gtcggctcat ccagcaccac gactttgggc
     1441 ttgacgatga gcgcgcgcgc gatggcgatg cgctgccgct ggccgccgga gaactcgtgc
     1501 ggatagcgcc cgaggagcgg ctccgcccgc ccgccttcgg tgagcccgac gtcgtagagc
     1561 gcctgcagta cacgcccgcg cctcgccgcg gcatcgagcc cgggctcgtg gatttcgagg
     1621 ccttcgccga tgatctgctc taccgtgcgt cttggggaga gcgacgagag cggatcctgg
     1681 aatacgacct gaatgtcctt gcgcatagta cggcgctccg cacgctgctg ccagcttcta
     1741 ttccctattc ggatgtctcc gtgggaagcc atcagcccga gcacggcaag cgccagggtg
     1801 gtcttcccgg agcccgattc gccgatcacc ccgagtgtct cgccgggagc gagatcgaaa
     1861 tctaccgacg ccaccgcggt gaagtttccg ctcttgaacc agccgcggat gccgggcagc
     1921 cgggtcggat agttcacccg gaccttgctg gcctcgacca gaactccagc tgacgcaggc
     1981 gcaatgtcgc gggcgggccg gctgttgacg agcttgcgcg tatagggatg ctgcggccgt
     2041 ccgatcacct gcgcggtggg tccctgctcc accagcacgc ctttctccat caccgcgacg
     2101 cggtcggcga aacgcctcac cagattcaag tcatgggtaa tgagcagcag cgccatcccg
     2161 gcctcggcgc gcagcttctc cagcagctcg aggatctgca ggcggatggt gacgtcgagc
     2221 gccgtggtcg gctcgtcggc gatcaggagc ttcgggctgc aggcgagcgc catggcgatc
     2281 atcgcgcgct ggcgctggcc gccggagagc tggtgcggat agctgtgcgc gcgccgctcg
     2341 ggctcgtcga tcccgacccg gcgcatcgcc tgcaccgcct ccttccatgc ggccgcgccg
     2401 cgcagattgg tgtgcagctc gagcgactcg acgatctggt cgccgaccgt gtagatcggg
     2461 ttgagcgccg tcatgggctc ctggaagatg accgcgatgt cccggccgcg cagctggtgc
     2521 agctcgtccg cggacatcgc gagcacgttc ttcccattga acacgatccg gccgctcagc
     2581 ctcgcgttct cgatgagccg cagcgtcgaa agcgccgtaa ccgtcttgcc cgacgccgac
     2641 tcccccacca gcgcgagctt ctcgcgcgca tccagggtga acgaggcgtt gtgtaccacc
     2701 cggcgcgggc cgaaatcgat gcacaggtcc ttgacctcga gaagactcat ggcgccgacc
     2761 cccttgtcgg gagggcgaac cggcaggcgc tcaagcttcg tcctccttca acaccttcct
     2821 tggatccaaa gcgtcccgaa gcgcatcgcc gatcaggatc aggaggagca gcgtcacgac
     2881 gagcacggcg aaggtggata gcgagatcca ccacgcatcc aggttgttct tgccctggtt
     2941 gaggagctcg cccaggctcg gcgtccccgg cggcacgccg aggccgagga agtcgaggct
     3001 ggtgagcgcg ccgatggccg cggccatctc gaaggggatg agcgtgacca ccggcgtgag
     3061 gctgttcggc aggatgtggc gccggatgat cttccaggtc gtctggccga gcgcgcgcgc
     3121 cgcccgcacg tagtcgagcg tgcggttgcg caggaattcg gcgcgcacgt aggcggcgat
     3181 gccgagccag ccgaacaccg acagcagaat caccagcagg ccgaagctcg cggagaaaag
     3241 cgacgagaag atgatcagca tgtaaagcgt tggcatcgcg ctccagatct cgttgaagcg
     3301 ctccatcgcg atgtcggtcc agccggcgaa gtagccctgg atggcgccat acagcgtgcc
     3361 gaccagggag acgatggcgg ttacgatgag cgcaaatacc accgagaggc gaaagccata
     3421 gagcaggcgc gcgagcaggt cgcgcccccg gtcgtcggta ccgagccggt tctcgccgct
     3481 cggcggcgcg gggctcggct cggaggcgaa atagttgatg gtgctatggt gatatcggtt
     3541 aagcgggaac acggcgatgt tcgcgccctt cgacaggttc tgctgaatca gcggatccag
     3601 gtagtcggtg ggcgtcgaaa agtcgccgcc gaaagtcgtc tcgggcagtg tctgcaggat
     3661 cgggagatac catctgccct cgtagcgcgc gacaagcggc ttgtcgttcg agagcacttc
     3721 ggcaagcagg cttacgccga aaagcaccgc gaacacgacg aggctcgtat accccaggcg
     3781 gttggcgcgg aagcgccgcc aggcgcgccg gccgggactc gcatggacgg cgggcgaaac
     3841 cggaaaatcg atggcgacgg cggggctcga catccgctag tcgaagaatt tcacgcgcgg
     3901 atcgacccag agatagctca gatccctcgc gagcgtggtg agcagcccga tgatcgtaaa
     3961 gacgaagagg ctgccgaaga cgaccggata atcgcgccgg atcacggact cgtaggagag
     4021 cagccccagc ccgtcgagcg agaagagcgt ctcgatgagc agcgagccgc tgaagaaagc
     4081 gccgacgaac gccgccggga agccggtcat gatcgggatc atggcgttgc ggaacacgtg
     4141 ccgccacagc acccggcgct catcgacgcc ctttgcgcgc gcagtgagca catagtgctt
     4201 gcggatctcc tcgaggaccg agttcttggt gaggatcgtg atgaccgcga agccgccgag
     4261 caccatggcg gtaaccggaa gcgcgatgtg ccagaagtaa tcaagaacct tgtctatggg
     4321 attcatgtct ccccaattga tggacgtaat gccgcgcaag gggaaccatt ggaggaacga
     4381 gccgccggca aagaaaacca gcagcaccac gcccatgacg aagctcggga tggcgtagcc
     4441 cagcagcacg acgaacgtcg ttaccgtgtc gaacggcgtg ccggctcgca cggccttggc
     4501 aatgccgagc ggcaccgaga tcaggtacac gaggaggaag gtccacacgc ccaggctgat
     4561 cgacaccggc agcttctcca ggatcagctc gccgaccgcc ttgttgtgga aaaagctctt
     4621 gccgaggtcg aagcgcgcga actgcccgat catctgcgcg aagcgctcgg gcgccggctt
     4681 gtcgaagccg tagagctgcc tgatctcctc gacgcgcacc gcgtccaccc cctgccgccc
     4741 gcggtagccg ccctccgccg cggccgggcc ctcgccgccc gcctcgaagc cctggaactg
     4801 ggccaccatc tgctcgaccg gccctcccgg aacgaactgg atcacgatga acgtgatgag
     4861 caggatcccg aaaagcgtcg ggatcatg
//
LOCUS       DASUNT010000159         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_109047, whole genome shotgun sequence.
ACCESSION   DASUNT010000159 DASUNT010000000
VERSION     DASUNT010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109047"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VFX94_03115"
     CDS             complement(<1..537)
                     /locus_tag="VFX94_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767200.1"
                     /translation="MAKGRKDHGTTHVRPDDIDPKHRWDRPLQAPGRTQVDFEERVDF
                     RRLHDYRLARVRAALAHSGLGALLSFDQHNIRYTTSTVIGEWARDKLTRYSLLTGNGE
                     PYIWDFGSAARHHKLYAPWLREDHCRAGMLGMRGATGADLTLFTQAAKEIRGILEEEG
                     VARMPLGVDVVEPPMLFAL"
     gene            581..1594
                     /locus_tag="VFX94_03120"
     CDS             581..1594
                     /locus_tag="VFX94_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX5767201.1"
                     /translation="MSEMLVPRERLERFTAACLEKLGVQPADAQLVAQTLVAANLRGV
                     DSHGVVRLPHYATRLRNGSVKPRPNITVERTGTSTAVIEGDAGMGQLVATRAMQEAIS
                     LARQTGVGAAVARNSSHCGACAWFVEMAVHEGMIGLALTHTDSIMVPPGMKRIFLGSN
                     PIAFGAPGEREPVIIDMSTTHVAWGKILVARQEGKPIPPDWGIDKDGKPTTDPHAVVG
                     LAPTGSHKGYALAAMVEILCAQLAGVPFGRHVTKMYGELDKPRNLGHFMLALDVSRFA
                     ERNMFFSQIAAFLDDIHAEGALAPGDPERRTAERREREGIPLGATVEELNKLAQELGV
                     AKL"
     gene            1600..2202
                     /locus_tag="VFX94_03125"
     CDS             1600..2202
                     /locus_tag="VFX94_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX5767202.1"
                     /translation="MFDLGGVVVRWDPDAIIASVFPDRKIHSKLKADVFAHPDWLELD
                     RGTLEREDAIARAAQRTGMAVEDIRRLVHAVPASLVVFPDTVDLLHRLKRQGYPLYCL
                     SNMHIASIEHLESEHTFWDLFDGRVISCRLQLCKPEPAIYKHLLREYGLRASETIFID
                     DVQKNLDAAAELGISTVRFRDPAQCEQDLRALGVNIPQPI"
BASE COUNT          377 a          754 c          718 g          381 t
ORIGIN      
        1 cagcgcgaag agcatcggcg gctcgaccac gtcgacgccg agcggcatcc tggcaacgcc
       61 ctcctcttcg aggatcccgc gaatttcctt tgccgcctgc gtaaagagcg tgagatccgc
      121 gccggtcgcg ccgcgcatcc ccagcatccc ggcgcggcag tggtcctcgc gcagccacgg
      181 cgcgtagagt ttgtggtggc gcgcggcgct gccgaagtcc cagatgtagg gctcgccgtt
      241 cccggtgaga agcgaatagc gcgtgagctt gtcgcgcgcc cactcgccga ttaccgtgct
      301 cgtggtgtag cggatattgt gctggtcgaa cgagagcagc gcgccgaggc ctgagtgcgc
      361 gagcgcggcg cgcacccgcg ccaggcggta gtcgtgcagc cggcggaaat cgactcgctc
      421 ctcgaagtcg acctgggtgc gccccggcgc ctgcagcggc cggtcccagc ggtgcttcgg
      481 atcaatgtcg tcgggacgca cgtgcgtcgt cccgtgatcc ttccttcctt tcgccatcct
      541 gcctcctcgc gccgccgatt ctagccccta aaattcgcac atgagcgaaa tgctggtgcc
      601 acgcgagcgg ctggaacggt tcaccgccgc gtgtctcgaa aagctcggcg tgcagcccgc
      661 cgatgcgcag ctcgtggcgc aaacgctggt cgccgcaaac ctgcgcggcg tcgacagcca
      721 cggcgtggta cgcctcccac attacgccac gcggctgcgc aacggttcgg tcaagccgcg
      781 tccgaacatc accgtcgagc gaaccgggac ttccaccgcc gtgatcgaag gcgatgccgg
      841 catgggacag ctcgtcgcga cgcgggcgat gcaggaagcc atttctctcg cgaggcagac
      901 cggtgtgggc gccgcggtgg cgcgcaacag ctcccattgc ggcgcgtgcg cctggttcgt
      961 cgagatggcg gtgcacgaag gcatgatcgg cctcgcgctc acccacacag attccatcat
     1021 ggtgccgccg ggaatgaagc gcatcttcct cggctccaac ccgatcgcgt tcggcgcgcc
     1081 cggagagcgc gagccggtga tcatcgacat gtccaccacg cacgtcgcgt ggggaaagat
     1141 cctggtcgcg cgtcaggaag gaaagccgat cccgcccgac tggggcatcg acaaggacgg
     1201 aaaaccgacc accgacccgc acgccgtagt cgggctcgcg cccaccggga gccacaaggg
     1261 ttacgcgctc gccgcgatgg tggagatcct gtgcgcgcag ctcgccggcg tgcccttcgg
     1321 ccggcacgtc accaagatgt acggcgagct cgacaagccg cgcaacctcg ggcatttcat
     1381 gctggcgctg gatgtttccc gtttcgcaga aaggaacatg ttcttctccc agattgcagc
     1441 atttctggac gacatccatg ccgaaggcgc tctggcccct ggtgatcctg agcggcggac
     1501 cgctgagcgg cgcgagcgcg aaggcattcc cctcggcgcc acggtggaag agctgaacaa
     1561 gctcgcccag gaacttggag tcgcgaagct ttgaacctcg tgttcgacct gggcggggtt
     1621 gtggtgcgct gggatcccga cgccatcatc gcttcggttt tccccgaccg taagatccac
     1681 tccaagctca aggctgacgt attcgcccac ccggactggc tcgagctcga tcgcggcacg
     1741 ctcgagcgcg aggatgcgat cgcccgcgcg gcgcagcgca ccgggatggc ggtggaagac
     1801 atccggcgac tcgtgcacgc ggtgccggct tcgctcgtcg tgtttcccga caccgtagac
     1861 ctgctccacc ggctcaagcg ccagggatac ccgctctatt gcctgtcgaa catgcacatc
     1921 gcctcgatcg agcacctcga gagcgagcac accttctggg accttttcga cggcagggtt
     1981 atttcctgcc gcttgcagct ctgcaagccc gagcccgcga tttacaagca cttgctgcga
     2041 gagtatgggc tgcgcgcgag cgagaccatc ttcatcgatg atgtccagaa gaacctggac
     2101 gcggcggctg agctcggtat cagtacggtg agatttcgcg accccgcgca gtgcgagcaa
     2161 gatttgcgcg cgctcggcgt gaatattccc cagccgatct agcgctcgcc ggcttccctc
     2221 gggcgcgtga
//
LOCUS       DASUNT010000160         5084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_459804, whole genome shotgun sequence.
ACCESSION   DASUNT010000160 DASUNT010000000
VERSION     DASUNT010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459804"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..762)
                     /locus_tag="VFX94_03130"
     CDS             complement(142..762)
                     /locus_tag="VFX94_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX5767203.1"
                     /translation="MIGQPAPDFTLPAVHREGDVSLADYRGKPLFLAIFAGLYCPFCR
                     RNIAQLGGTQQKLHDIGVETLGVVATEVDNARLYFKYRPARVPLVADPELTMHRAYSL
                     PRVPMDETLEKAFHTVPLDPLGNLPEPMPLAQLGPMLDRADGFQHTAADLRDKERQFP
                     QLKGQFLIDADGIVCWMNVECSNEGFTGVGKFPSDDELLAAAERIA"
     gene            complement(775..3750)
                     /locus_tag="VFX94_03135"
     CDS             complement(775..3750)
                     /locus_tag="VFX94_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5767204.1"
                     /translation="MSASPQEADRRPVTVLFADLSGFTALAERLDPEDVRALQTELFD
                     TLRGALEPLGAFIEKFVGDAVVAVFGAPQAHEDDPERAMRAALDMHRRAAALSARWQR
                     RLGSPLALHIGVNTGRVVAGRLGSDPQASYAVTGDAVNTASRLQSAAQAGETLVSRAT
                     YLLAQHAFSFAPAGEVSLKGKAEPLAVYRLVGRADAPRAARGLASLGLAAPLIGRDAE
                     LAQIRAAFNAAVAGRAQVLGIRGEPGNGKSRLVAEFVAQLRSGDGQRLTVRRAACSSL
                     GEPPYGVMAQFFRKGYGLEPTDTLETARRKIEEGLRALGAGKDEALGVAPIVGYVLGI
                     AADGAARDIEPEQLSRQILYMLRLVFDRRLAKGPLLLVVEDLHWADTASLEGLRILAD
                     WLADRPLMLLATYRSAFAGIGDTRAAHSEIRLNPLPSAEADNLLRAFFGEALPESLQR
                     QLVERCGGNPFYLEELVRGLVARGAVAREQGRWKSVAETSALDIPPTIEGLLLSRIDR
                     LAPKLRATLQQASVLGPVFDADLLARIGGAPSLLNELCEAGVLESCGEASFAFVHALL
                     HDVVYQNLLQRRRAELHGRLGTELERLHGTHPQRLEELEALGHHWSLSEDRARGARYL
                     FAAGDWARGIYANDDAVRHYRRALSALESCESCESEILEVRERLGDLLGLIGHRAEAI
                     EHYSAVHQAAVDPVTQARLCRKSGALHWDAGERERGLAWFQQGLALLQNGSEPIERAH
                     LYQEMGRLAFRSGDNRGALNWAERALGEAERSRADSEAAGAVAHALNTLGVAMARLGR
                     DREAVAHIERSVGIALERGMLQAACRSYANLGVLYASLDPRRAIETCLTGLETAKKIG
                     DVGFQSRLYANLAVAYCALTDRCDIDGLRAAEAAIALDRKLGQLDHLAVPLIVLGQIH
                     QCHGEPERALEYYREALALAEEIREPQLLFPCYDGLATLFLDLGNTAEAERYLIKAAD
                     VAERAGLDRDSLVVLPFLC"
     gene            3770..4435
                     /locus_tag="VFX94_03140"
     CDS             3770..4435
                     /locus_tag="VFX94_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX5767205.1"
                     /translation="MKAPIQPGERAPEFSLAAINREGVVSAADYRGRAVMIGFFRGLH
                     CPFCRRQIAQLGAAQPTLATLEVETLAVINTPLERARLYFRYRPTPVTLLSDPECETH
                     QAFGIPRIEFVEPGGAPAKWPRTRPGDFAAARIDPTGELGAPTQPMEANTVLNRKDGF
                     ELDAADKEIFERHGTQFAGQFLIDREGIARWSWTEAPHSPNELCRFPSAQEMLEAAQQ
                     LNR"
     gene            complement(4549..>5084)
                     /locus_tag="VFX94_03145"
     CDS             complement(4549..>5084)
                     /locus_tag="VFX94_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5767206.1"
                     /translation="PKLTLEQMNAAQRAVAAEITAGPRGEVRGPFIALIHNPELASRL
                     QTLGEFLRWKGKLPARLKELAVLVTARRWTCQHEWVMHSKLALEAGLATHIVNAIAVD
                     REPANLSSEENAVYRFCRDLHATGRAGDEAFREIEKRFGLDGALELIALNGYYTLMAM
                     VLNTAGLPLPGNANPPL"
BASE COUNT          803 a         1678 c         1807 g          796 t
ORIGIN      
        1 cgatattgcg acggcagaac gggcaataca ggccgatgaa gactgcgagc agcaggtggc
       61 tcttgccgcg gtagtcggcg agcgatattt cgccgtcgcg gtgcaccgcg ggcagggtga
      121 agtcgggagc gggccgcccg atcacgcgat tcgctcagcg gcggcgagaa gctcgtcgtc
      181 gctcggaaat ttgccaacgc ctgtgaagcc ttcattgctg cactcgacgt tcatccagca
      241 cacgatgcca tccgcgtcga tgaggaactg gcccttgagc tgaggaaact ggcgctcctt
      301 gtcgcgcagg tcggcggcgg tgtgctgaaa gccgtcggcg cggtcgagca tagggccgag
      361 ctgggcgagg ggcatgggtt cggggagatt gcccagggga tcgagcggca cggtgtggaa
      421 ggccttctcg agtgtctcgt ccatcggcac gcgcggcagg gagtaagcgc ggtgcatggt
      481 gagctccgga tcggcgacga ggggaacgcg cgcggggcgg tacttgaagt agagccgcgc
      541 gttgtcgacc tcggtggcga cgacgcccaa agtctcgacc ccaatatcgt gcagcttctg
      601 ctgggtgccg ccgagctgcg caatgttgcg gcggcagaag ggacagtaaa gaccggcaaa
      661 gatggcgagg aagagcggct taccgcggta atcggcgagc gagacgtcgc cctcgcgatg
      721 taccgccgga agggtgaagt caggggcggg ttgtccaatc atgtcctggt cctcctaaca
      781 gaggaacggc agcacgacga gggagtcgcg gtcgaggccg gcgcgctcgg cgacatccgc
      841 cgccttgatg agatagcgct cggcctcggc ggtgttgccg aggtcgagga agagcgttgc
      901 gagcccgtcg tagcagggaa agaggagctg gggctcgcgg atttcctcgg cgagcgcgag
      961 cgcctcgcgg tagtactcga gggctcgttc gggctcgccg tggcactggt ggatctggcc
     1021 aagcacgatg agcggcacgg cgaggtgatc gagctggccg agcttgcggt cgagcgcgat
     1081 cgccgcctcg gcggcgcgca ggccgtcgat gtcgcagcgg tcggtgagcg cgcagtaggc
     1141 gacggcgagg ttggcgtaga ggcgcgactg gaacccgacg tcgccgatct tcttcgcggt
     1201 ttcgagcccg gtgaggcagg tctcgatcgc gcgtctcggg tcgaggctcg catagagcac
     1261 gccgaggttc gcgtagctgc ggcacgccgc ctggagcatg ccgcgctcga gcgcgatgcc
     1321 gacgctgcgc tcgatgtgcg cgacggcctc ccggtcgcgg ccgagccgcg ccatggcgac
     1381 cccgagcgtg ttgagcgcgt gcgccaccgc gccggcggct tcgctgtcgg cacgggaacg
     1441 ctcggcttcg ccgagcgcgc gctcggccca gttgagcgcg ccacggttgt cgccgctgcg
     1501 gaacgcgagc cttcccatct cctggtagag atgggcgcgc tcgattggtt cgcttccgtt
     1561 ctgcagcagc gcgaggccct gctggaacca agctagtcct cgctcgcgct cgcccgcgtc
     1621 ccagtggagc gcgccgctct tgcggcaaag gcgggcctgc gttacgggat cgaccgccgc
     1681 ttgatggaca gcggagtagt gctcgatcgc ttcggcgcga tgcccgataa gtccgaggag
     1741 gtcgcccagg cgctcgcgaa cttcgaggat ttccgactcg cagctctcgc agctttcgag
     1801 ggccgaaagc gcccgccggt agtggcgcac cgcatcgtcg ttggcgtaga ttccgcgcgc
     1861 ccagtcgccg gccgcgaaga gatagcgggc gccgcgtgcc cggtcttcgc tcaggctcca
     1921 atggtggcca agcgcttcga gctcttccag ccgctgcgga tgcgtgccgt gcagccgctc
     1981 aagctccgtg cccaatcgcc cgtggagctc ggcgcgccgg cgctgcagca ggttctggta
     2041 gaccacgtcg tgcagcagcg cgtggacgaa ggcgaagctc gcctcgccgc aggattcgag
     2101 cacgccggcc tcgcacagct cgttcagtag cgacggcgca ccgccgatgc gcgcgaggag
     2161 atcggcgtcg aagaccggtc cgaggaccga cgcttgctgg agcgtagcgc gcagcttcgg
     2221 cgccagccgg tcgatgcgcg aaagcagcag cccttcgatc gtgggcggaa tatcgagcgc
     2281 ggacgtctcg gcaacgcttt tccagcgccc ttgctcgcgc gcgacggctc cgcgcgcgac
     2341 cagcccgcgc actagctctt cgaggtagaa agggttgccg ccgcagcgct cgacgagctg
     2401 gcgctgcagg ctctcgggca gcgcctcgcc aaagaaggcg cgcagcaggt tgtcggcctc
     2461 ggccgacggc agcgggttga gccggatctc gctatgcgcg gcgcgtgtgt ctccgatgcc
     2521 cgcaaacgcc gagcggtagg tggcgagaag catgagcggc cggtcggcga gccagtcggc
     2581 gagtatgcgc agcccttcga gcgaggctgt gtcggcccag tgcaggtctt cgaccacgag
     2641 aagcagcggc cccttcgcga gccgtcggtc gaacacgagc ctcagcatgt agaggatctg
     2701 gcggctgagc tgctcgggct cgatgtcgcg ggcggcgccg tcggccgcga tgccgagcac
     2761 atagcccacg atcggcgcca cgccgagagc ttcgtccttt cctgcgccga gcgcgcgcag
     2821 gccttcttcg atcttgcgcc gcgcggtctc gagcgtatcg gtcggctcca gcccatagcc
     2881 tttgcgaaag aactgcgcca ttacgccgta tggcggctcg ccgagcgagg agcaggcagc
     2941 gcgccgcacc gtcaaccttt ggccgtcgcc ggaccggagc tgcgcgacga actcggcgac
     3001 caggcgggat ttgccgtttc ccggctcgcc acggatgccg agcacttgcg cgcggccggc
     3061 gactgcggcg ttgaacgcgg cccggatctg cgcgagctcc gcatcgcgtc cgataagcgg
     3121 cgcggccagt ccgagcgatg ccagcccgcg cgccgcgcgc ggcgcatccg cccggccgac
     3181 gaggcgatac accgcgagcg gctcagcttt gcccttcagc gagacctcgc ccgcaggcgc
     3241 aaacgagaac gcgtgctgcg cgaggaggta ggtcgcgcgg ctgactagcg tttcgccggc
     3301 ttgcgccgcc gactgcaggc gcgacgcagt attcaccgcg tcgccggtga cggcatagct
     3361 agcctgcgga tcggagccga gtcgtcccgc cacaacgcgg ccggtattca cgccgatgtg
     3421 aagagcgagc ggacttccga gccggcgctg ccagcgcgcg ctgagcgccg cggcgcgccg
     3481 gtgcatgtcg agcgcggcgc gcattgcgcg ctccggatcg tcctcgtgcg cctgcggcgc
     3541 gccgaacacc gcgacgaccg cgtcgccgac gaacttctcg ataaacgcgc cgagcggctc
     3601 gagcgcgccg cgcagcgtat cgaaaagctc ggtctgcagc gcgcgcacgt cctccgggtc
     3661 gagccgctcg gcgagcgcgg tgaacccgga gagatcggcg aacagcacgg tcaccgggcg
     3721 gcggtcggcc tcttgcgggg aagcgctcat gccacaattg tccctctcta tgaaggcccc
     3781 catccagcca ggcgagcgcg cgccggagtt ttcgcttgcc gcgatcaacc gcgaaggcgt
     3841 ggtctcggcg gcggactacc ggggacgcgc ggtcatgatc ggctttttcc gcggcctgca
     3901 ctgccccttc tgccgccgcc agatcgctca gctcggcgcg gcgcagccca cgctcgcgac
     3961 cctagaggtc gagacgctgg cggtgatcaa tacgccgctc gagcgggcgc gcctctattt
     4021 ccgctaccgg cccacccccg tcacgctcct gagcgatcct gaatgcgaga cacaccaggc
     4081 gttcggcatt ccgcggatcg agttcgtcga gcccggcgga gcaccggcga aatggccgcg
     4141 cacacgcccc ggcgatttcg ccgcggcgcg catcgatccg accggcgagc tgggagcgcc
     4201 cacgcagccg atggaggcga acaccgtgct caaccgcaag gacggcttcg agctcgacgc
     4261 ggccgacaaa gagatcttcg aaagacacgg cacccagttc gcaggccagt tcctaatcga
     4321 ccgggaaggc atcgcgcgct ggagctggac cgaggcgccg cactccccca acgagttgtg
     4381 ccgcttcccc agcgctcagg aaatgctcga ggctgcgcag caactgaacc gctaggccgt
     4441 cagcgcggcc cgcgcagtac cggctcgcgg tgaacctcaa gaccgccaga gagatgggcc
     4501 aaccgcggtt tccggaactg ctctgacccc ttttgttttg ccgttacttc atagcggtgg
     4561 gttggcgttg ccgggtaggg ggaggccggc ggtgttgaga accatcgcca tcagcgtgta
     4621 gtagccgttg agtgcgatga gctcgagggc tccgtcaagg ccgaagcgct tctcgatttc
     4681 gcgaaaggct tcgtcgccgg cacgccctgt agcgtggagg tcgcggcaga atcgatagac
     4741 cgcgttctcc tctgaagaaa gattcgctgg ctcgcggtcc acagcgatcg cattgacgat
     4801 gtgcgtcgcc aagcccgctt cgagggcgag ctttgagtgc atcacccatt cgtgctggca
     4861 ggtccagcgg cgagcggtga ccagcaccgc gagctccttg agccgcgccg gcagcttgcc
     4921 cttccagcgc aggaattcac ccagcgtctg cagtcggctg gccagctccg ggttatggat
     4981 gagagctatg aacgggccgc gtacctcgcc acgcgggccg gcggtgatct cggcggcgac
     5041 tgcgcgctgc gcggcgttca tctgctcgag cgtgagcttc ggga
//
LOCUS       DASUNT010000161         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_635099, whole genome shotgun sequence.
ACCESSION   DASUNT010000161 DASUNT010000000
VERSION     DASUNT010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635099"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="VFX94_03150"
     CDS             complement(<1..1099)
                     /locus_tag="VFX94_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HEX5767207.1"
                     /translation="MKTPVAIVGAGPAGLLLSHLLHLDGVDSVVLESRTRQEIEATIR
                     AGVLEQGTVDLLVGSGVGERMKREGFVHEGIVLRFAGASHRIALTELTGRSITVYAQH
                     EVIKDLVKARLHAGGRLFFGVKDTALYDLDSASPKVRFHREQSVHEIESQFVAGCDGF
                     HGVSRPSIPSRARREFARTYPFGWFGILVEAPPSTAELIYAHHERGFALVSTRSPQIQ
                     RLYFQCDPEDDLKNWPDARIWEELHARLATREPWKLAEGGIFQRGIIAMRSFVVEPMR
                     YGRLFLAGDSAHIVPPTGAKGLNLAVHDVRVLARAFVDYFKTGSEDGLNAYSATALKR
                     VWRAEHFSWWMTSMLHRFHNDTPFQHRLQLAE"
     gene            complement(1096..1482)
                     /locus_tag="VFX94_03155"
     CDS             complement(1096..1482)
                     /locus_tag="VFX94_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX5767208.1"
                     /translation="MSELATLFALSFAAATLIPLPSEAALFAYVHVYPERAALAVAIA
                     SAGNTAGGMTSYALGRLIPAQKVDLKAVHWLRRYGAPATLLAWLPIVGDALCVAAGWL
                     RIHWAAAFGFMATGRLARYIAVASLA"
     gene            1500..>2033
                     /locus_tag="VFX94_03160"
     CDS             1500..>2033
                     /locus_tag="VFX94_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5767209.1"
                     /translation="MSKGYLERILKARVYDVASESPLEPAPALSRRLGNRLLLKREDL
                     QPIFSFKLRGAYNKMAGLPRSRLARGVIAASAGNHAQGVALAAQRLGCRAVIVMPVTT
                     PRIKVNAVAARGAEVVLHGDSYAEAYKKSLELKSRRSLTFVHPYDDPDVIAGQGTIGM
                     EILRQHPRPIDAIFVPVG"
BASE COUNT          345 a          670 c          714 g          304 t
ORIGIN      
        1 gctctgcaag ctgcaggcgg tgctggaacg gcgtatcgtt gtggaagcgg tggagcatgg
       61 aggtcatcca ccacgaaaaa tgctccgcgc gccacacgcg cttgagtgcg gtcgcggaat
      121 aggcgttcag gccgtcttcg ctcccggtct tgaagtagtc gacgaatgcg cgcgcgagca
      181 cgcgcacatc gtgcacggcg aggttgagcc ctttcgcgcc cgtgggcggc acgatgtgcg
      241 cggagtcgcc cgcgaggaac agcctgccgt agcgcatcgg ctcgaccacg aagctcctca
      301 ttgcgatgat gccgcgctgg aagatgcccc cctcggcgag cttccagggc tcgcgtgtgg
      361 cgaggcgagc gtgcagctcc tcccagatgc gcgcgtcggg ccagttcttg aggtcgtctt
      421 ccgggtcgca ctggaagtac aggcgctgga tctgcggcga gcgcgtgctg accagcgcga
      481 agccccgctc gtggtgcgcg tagatgagct ccgcggtcga gggcggcgct tcgaccagga
      541 tgccgaacca gccgaacggg taagtgcgcg cgaactcgcg ccgggctcgc gacggaatcg
      601 agggacgcga tacgccgtgg aagccgtcgc agccggcgac gaactgcgac tcgatctcgt
      661 ggacgctctg ctcgcggtgg aaacgcacct tcggcgaggc cgagtcgagg tcgtagagcg
      721 ccgtgtcctt cacgccgaag aaaagcctgc cgcccgcgtg gaggcgcgcc ttgacgagat
      781 ccttgatcac ctcgtgctgt gcgtagaccg tgatcgagcg cccggtgagc tcggtaagcg
      841 cgatgcggtg gctcgcgccg gcgaagcgca gcacgatgcc ctcgtgcacg aacccctcgc
      901 gcttcatgcg ctctcccacg ccgctcccga ccaggaggtc gaccgtgccc tgctcgagca
      961 ccccggcgcg gatggtggcc tcgatctcct ggcgggtgcg cgactcgagc acgaccgagt
     1021 cgactccgtc gaggtggagc aggtgcgaaa gcagaagtcc cgccggcccg gcgccgacga
     1081 tcgccacggg tgttttcatg cgaggctcgc caccgcaatg tagcgcgcca gccggccggt
     1141 ggccatgaag ccgaaggcgg cggcccagtg gatccgcagc cagcccgcag cgacgcagag
     1201 cgcgtcgccg acgatcggca gccaggcgag gagcgtggcc ggcgcgccgt agcggcgaag
     1261 ccaatgcacg gctttcaggt cgaccttctg ggcgggaatc aaccttccca aggcatagga
     1321 ggtcatgccg ccggcggtgt tgccggcgct cgcgatcgcc actgcgagcg ctgcgcgctc
     1381 gggatagaca tgcacgtagg cgaacagcgc tgcctcggaa ggcagaggga tcaacgtcgc
     1441 cgcggcgaaa ctcaacgcga acagggtcgc gagttccgac atgctacatt cggccggctt
     1501 tgagcaaggg ctacctcgag cgaattctga aggcgcgggt ctacgacgtg gcgagcgaat
     1561 cgccgctcga gccggcgccg gcgctttcca ggcgcctcgg caatcgcctg ctcctcaagc
     1621 gagaagacct gcagccgata ttctcgttca agctgcgtgg cgcctacaac aagatggccg
     1681 gattaccgcg cagccgcctc gcgcgcggcg tgatcgcggc gagcgcgggg aaccacgcgc
     1741 aaggcgtcgc gctcgccgcg cagcgcctgg gctgccgcgc ggtgatcgtg atgccggtca
     1801 ctacgccacg catcaaggtg aacgcggtgg cggcgcgcgg cgcggaggtg gttctgcatg
     1861 gcgactccta cgcagaggcg tacaagaaat cgctcgagct gaaaagcagg cgcagcctca
     1921 ccttcgtcca tccgtacgac gacccggacg tgattgccgg ccagggcacg atcggcatgg
     1981 agattctgcg ccagcacccc cggccgatcg acgcgatctt cgtcccggtg ggc
//
LOCUS       DASUNT010000162         2274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_635322, whole genome shotgun sequence.
ACCESSION   DASUNT010000162 DASUNT010000000
VERSION     DASUNT010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635322"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VFX94_03165"
     CDS             <1..1161
                     /locus_tag="VFX94_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767210.1"
                     /translation="MKANLMTRLLPRTFKCAFLALGAVLISAGSAGAGSGASPDDVAR
                     FMAGLPPKAESPLVPLTQTPAWQHHARVFSSAWARLDQRQLARVRAWSGENLKESQAN
                     MFYMFSGPDFLYADAFFPKASTYVLSGLEPVSQVPDPTMLARRSLASALGELRSSLNS
                     VLSYSFFQTKHMRRDLNSGQMTGTLPILYVFLARSGKTIHEVSFVSLDKDGTVLPSQG
                     SANAADQGVKIVFSKGEGPRQTLYYFRTDVSNGGVANSGFLRFCEHLGTGDSFVKSAS
                     YLMHSDGFSKVRDFLLTRSVTLVQDDSGVPVRFFEANGWMLRPHGRYRGPIRLFGSRY
                     QAQLKELFRKGPSSPLAFGIGYRWRANDSNLMVGVRKIATSAQPSVPAEASR"
     gene            complement(1175..>2274)
                     /locus_tag="VFX94_03170"
     CDS             complement(1175..>2274)
                     /locus_tag="VFX94_03170"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX5767211.1"
                     /translation="DADPQDARRAAELCKADLVSGMVGEFPELQGLMGRYLAEAEHLD
                     PKVARAIELHYKPKGPSDTVPKEEEGDAVAIAVALADKLDTLVGFWAIGEKPTGSGDP
                     YQLRRAALGLIRIVLENDLRVPLFRTLRNHAMAVIKGMENRNFLGALKTVEQLPSQAR
                     AAKELARHEIALNAVFGTSYVKEFERAVAISDDLLAFFADRLKVHLREKGARHDLIDA
                     LFALPGQDDLALIVKRVEALGAFLKTEDGEHLLVGVKRATNILRDEEKKDGRSYAGDY
                     DVKLLTASEELALAAAIESVSADTRAAINVENFKGAMNALAELRRPVDAFFDKVTVNT
                     DDPALRANRLALLSQIRAATLNVADFSKIAG"
BASE COUNT          357 a          767 c          742 g          408 t
ORIGIN      
        1 atgaaggcga acctcatgac gagactgctc ccccgcacct tcaagtgcgc cttcctcgcc
       61 ctgggcgccg tgctgatctc ggcgggctcc gccggtgccg gcagcggggc cagtcccgac
      121 gacgtcgcgc gcttcatggc cgggctgccg ccgaaggcgg agtcgccgct ggtgccactg
      181 acgcagacgc cagcatggca gcaccacgcg cgcgtgttca gttcggcgtg ggcccggctc
      241 gatcagcggc agctcgcgcg cgtccgcgcc tggtcgggcg agaacctgaa ggagagtcag
      301 gcgaacatgt tctacatgtt cagcggcccg gacttcctgt acgccgatgc cttcttcccg
      361 aaggcgtcca cctacgtcct gagcgggctc gagccggtca gccaggtccc cgatccgacg
      421 atgctggcgc gccgctcgct ggcctcggcg cttggggagc tgcgctcttc gctcaactcg
      481 gtgctgagct acagcttctt ccagaccaag cacatgcgcc gcgacctgaa ctcggggcag
      541 atgacgggca cgctgcccat cctctacgtg ttcctcgcgc gctcgggcaa gacgatccac
      601 gaggtgagct tcgtcagcct cgacaaggac ggcaccgtgc tgccgagcca agggtccgcc
      661 aacgccgccg accaaggcgt gaagatcgtg ttctcgaagg gcgaggggcc gcgccagacg
      721 ctctactact tccgcaccga cgtctccaac ggcggcgttg ccaacagcgg cttcctgcgc
      781 ttctgcgagc acctgggcac tggcgacagc ttcgtgaaga gtgcctccta cctcatgcac
      841 tcggacggct tctccaaagt gcgcgatttc ctcctcacgc gcagcgtgac gctcgtgcag
      901 gacgactcgg gcgtgccggt gcgcttcttc gaagcgaacg gctggatgct gcgtccccac
      961 ggccgctacc gcggcccgat ccgcctgttc gggagccgct accaggcgca gctgaaggag
     1021 ctgttccgca agggcccgtc ctcgccgctc gcgttcggca tcggctatcg ctggcgcgcg
     1081 aacgactcga atttgatggt cggcgtgaga aagatcgcga cgtcagcgca gccgagtgta
     1141 ccggcggagg catctcgctg agcggggaat ccgctcagcc cgcgatcttg gaaaagtcgg
     1201 cgacgttcag cgtcgccgcg cgtatttgcg agagcagcgc aaggcgattg gcgcggagcg
     1261 cgggatcgtc ggtgttgacc gtcaccttgt cgaagaacgc gtcgaccggg cggcgcagct
     1321 cggcgagcgc gttcatggcg cccttgaagt tctcgacgtt gatggcggcg cgcgtgtcgg
     1381 cgctgacgct ctcgatggcg gcggcgagcg ccagctcctc ggacgcggtg agcagcttca
     1441 cgtcgtagtc gccggcgtag ctgcgaccgt ccttcttctc ctcgtcgcgc aggatgttgg
     1501 tggcgcgctt gacgccgacg agcaggtgct cgccgtcttc ggttttcagg aacgcgccga
     1561 gtgcctcgac gcgcttgacg atgagcgcga ggtcgtcctg gccgggtagc gcgaacaggg
     1621 cgtcgatgag gtcgtggcgc gcacccttct cgcgcagatg gaccttgagg cgatcggcga
     1681 agaaggcgag gaggtcgtct gagattgcaa ctgctcgctc gaactccttt acgtacgaag
     1741 tgccaaatac cgcattcaat gcgatctcat gccgagcaag ctcctttgct gcacgtgctt
     1801 ggctaggcag ttgctcgacc gttttaagcg cgcccaggaa gttgcgattc tccatccctt
     1861 tgatcactgc catggcgtga ttacgcaaag ttcgaaatag aggcacccgc agatcgtttt
     1921 cgagcacgat gcgaatgagc cccagcgccg cgcgccgcaa ctgatacgga tcgccgctgc
     1981 ccgtcggctt ctcgccgatg gcccagaagc ccaccagtgt gtcgagctta tccgccagcg
     2041 cgaccgcgat cgccaccgcg tcgccctcct cctccttggg cacggtgtcg ctggggccct
     2101 tgggcttgta gtgcagctcg atcgcgcgcg cgaccttggg gtcgaggtgc tcggcctccg
     2161 cgagatagcg gcccatcagc ccctgcagct cgggaaactc gccgaccatg ccggacacga
     2221 ggtccgcctt gcagagctcc gccgcgcgcc gcgcatcctg cggatcggca tcca
//
LOCUS       DASUNT010000163         2560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_373188, whole genome shotgun sequence.
ACCESSION   DASUNT010000163 DASUNT010000000
VERSION     DASUNT010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373188"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VFX94_03175"
     CDS             complement(<1..310)
                     /locus_tag="VFX94_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767212.1"
                     /translation="MLKEILGVADDPPAKRRWFHDEYFDLFVWQADGEVTLFQLCYGG
                     DASERALVWDKERGFFHDGPASRDVVALFEDAAAALPADLRAAIREKVHEFAGLQLSV
                     V"
     gene            614..>2560
                     /locus_tag="VFX94_03180"
     CDS             614..>2560
                     /locus_tag="VFX94_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HEX5767213.1"
                     /translation="MYQEVTVMRTTSGNSGRHEDVLRSLAQFSKEHRAARWSGTFSAF
                     LETVFPADARGIARPSHQYIWDMMRAEGFEDVNGRLRCQLFEDELYGIGDTIERVVDY
                     FKAAAAGSEVGRRLLLLLGPPSGGKSSMVILLKRGLEEYSHTDAGALYGIHGCPVHES
                     PLHLVPHTLRGQFRSTYAVDVTGELCPFCATRLQHEFAGDFMQVPVERIFISEAGRVG
                     VGTYAPHDPSTADIADLVGSVDLSKVSEFGDEGDPRAWSWSGAVFAASRGVLEMIEIL
                     KVKREFLYLLLTLTQEKNVKVSRFPLIHLDETILAHTNLAEFRKFLQESENEALLDRM
                     VIVQVPYTLSYKEEARIYRKLVSSTQAFREVHLDPHALHVAAVFSVLTRLAEGEEREA
                     DNVKKVRLYAGEDVEGVSQSQSEVEKMRAKNPDEGLAGVSPRFVINALSNAIIQSQAK
                     SLTTMNVLIALKDAIDSDVRIDGKKKRKWIDFLVTVRKDFYNRWVKEDVHKALFVSFE
                     DEAQQLLEKYLDEVEATLDNRTIKDPITNEERKPDERFLRSVEEKIKISESGKLSFRQ
                     EVVRKAMGAYKRGEKFTLDNHAPLHDAVEQYLFEQRRDVLRLVSSASRPDEETRQKIS
                     VVEKRLVDEYGYDSHSAREALNYVT"
BASE COUNT          483 a          826 c          827 g          424 t
ORIGIN      
        1 acaccacgga aagctggagg ccggcgaact cgtgcacctt ttcgcggatc gcggccctca
       61 ggtcggcggg gagcgccgcg gccgcatcct caaacagcgc aaccacatcc cgcgatgcgg
      121 gaccgtcatg gaagaagcca cgctccttgt cccagacgag cgcgcgctcg ctcgcgtcgc
      181 cgccgtagca gagctggaac agcgtcacct cgccgtccgc ctgccagacg aaaaggtcga
      241 aatactcgtc gtggaaccag cgccgtttgg ccggcggatc gtcggcgacg cctaggattt
      301 ctttcagcat attctcgtca acggtttgac cgtaggcacg ttgctcggtt ctgtcgccga
      361 aacgttaccc gcggtagcaa cggctttcct gcagcgcggc gaatccgctc tcggtcggga
      421 aacttcccgg cgcgcgccca ttctattacg tcaaccgaat tttcgtagac ccgttgcacg
      481 gtgcgttact tgctctcacg gcatgacggg cgctctgctc agggcatggc gggcgcgtgt
      541 cagatgactg cagcggacct gcaagcattg ggatacacat tcttgcttgc tctaagatgt
      601 tgagaggatc actatgtacc aagaggtgac cgtgatgcga acgacgagcg gaaacagcgg
      661 gcgccacgag gatgtgctca ggtccctggc gcagttcagc aaggagcacc gtgcggcgcg
      721 ttggtcggga acgttctccg cgttcctcga gaccgtgttc cccgccgatg cgcgcggcat
      781 tgcgcgcccg agccaccagt acatctggga catgatgcgc gccgagggct tcgaggacgt
      841 caacggccgg ctgcgctgcc agcttttcga ggatgagctc tacggtatcg gcgacacgat
      901 cgagcgcgta gtcgactact tcaaggccgc cgccgccggg tccgaagtcg gcaggcgact
      961 gctgctgctg ctagggccgc cctcgggcgg caagtcgagc atggtcatcc tgctcaagcg
     1021 cggcctggaa gagtacagcc acacggacgc cggcgcgctc tatggcatcc acggctgccc
     1081 ggtgcacgag tcgccgctgc acctcgtgcc gcacacgctg cgcgggcagt tccgctccac
     1141 ctacgcggtc gatgtgacgg gcgagctctg cccgttctgc gccacgcgcc tgcagcacga
     1201 attcgccggc gacttcatgc aggtgccggt cgagcggatc ttcatttcgg aggccggccg
     1261 ggtcggcgta ggcacttacg cgccccacga cccgagcacc gccgacatcg cagacctcgt
     1321 cggctcggtg gacctctcga aggtgtccga attcggcgac gaaggcgacc cgcgcgcctg
     1381 gtcgtggtcg ggggcggtat tcgcggcgag ccgcggggtt ctcgagatga tcgagatcct
     1441 caaggtgaag cgcgagttcc tgtacctgct actcactctc acgcaggaga aaaacgtcaa
     1501 ggtgtcgcgc ttcccgctga tccacctgga cgagacgatc ctcgcgcaca ccaacctcgc
     1561 cgagttccgc aagttcctgc aggaatccga gaacgaggcg ctgctcgacc gcatggtgat
     1621 cgtgcaggtg ccctataccc tctcgtacaa ggaagaggcg aggatctacc gcaagctcgt
     1681 ctcctccacc caggcgttcc gcgaggtgca cctcgacccg cacgcgctgc acgtcgcggc
     1741 ggtgttcagc gtgctcacgc gcctcgccga gggcgaggag cgcgaggcgg acaacgtgaa
     1801 aaaggtgcgc ctgtacgccg gcgaggacgt cgaaggcgtg tcgcagtcgc agtccgaagt
     1861 ggagaagatg cgcgccaaga accccgacga ggggctcgcc ggggtttcgc cgcgcttcgt
     1921 gatcaatgcg ctctccaacg ccatcatcca gtcgcaggcg aagagcctca ccaccatgaa
     1981 cgtgctgatc gcgctcaagg acgccatcga ctccgatgtg cgcatcgacg gcaagaagaa
     2041 gcgcaagtgg atcgacttcc tggtgacggt gcgcaaggac ttctacaacc gctgggtcaa
     2101 ggaggacgtg cacaaggcgc ttttcgtctc gttcgaggac gaggcgcagc agctgctcga
     2161 gaagtacctc gacgaggtcg aggcgaccct cgacaaccgc accatcaagg acccgatcac
     2221 gaacgaggaa cgcaagcccg acgagcgctt cctgcgctcg gtggaggaga agatcaagat
     2281 ctccgagtcg ggcaagcttt ccttccgcca ggaggtggtg agaaaggcga tgggcgccta
     2341 caagcgcggc gagaagttca cgctcgacaa ccacgcgccg ctgcacgacg cggtcgagca
     2401 gtaccttttc gagcagcgcc gcgacgtgct gcggctggtg tcctcggcgt cgcgccccga
     2461 cgaggagacg cgccagaaga tctcggtggt cgagaagcgg ctggtggacg agtacggcta
     2521 cgacagccac agcgcgcgcg aggcgctgaa ctacgtcacc
//
LOCUS       DASUNT010000164         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_21633, whole genome shotgun sequence.
ACCESSION   DASUNT010000164 DASUNT010000000
VERSION     DASUNT010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21633"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFX94_03185"
     CDS             complement(<1..230)
                     /locus_tag="VFX94_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEX5767214.1"
                     /translation="MNIDWAHFTPWASLAGGVLIGISAAMLVLLNGRVAGISGIIGGL
                     FRPALPDLGWRFAFLAGLVIAPSLYGAFTWLSP"
     gene            229..885
                     /locus_tag="VFX94_03190"
     CDS             229..885
                     /locus_tag="VFX94_03190"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5767215.1"
                     /translation="MKNHPVVRAIVMAAALASCAAAAQTPHTHRHGFGDAEKWSHIFD
                     DPKRDAWQKPHEVISALALKSDALVADIGAGTGYFSVRLANMHPRATVYAVDVEADMV
                     RYLGERASREGIKNLKPVAGLAEDPRLPEKVDLALFVDVYHHIERREAYFRRLAGSLK
                     PGARVAVIDFRLDSPEGPPKAARVAPERVKKELAAAGYALEAEHGFLPHQYFLVFSPR
                     "
     gene            complement(865..1521)
                     /locus_tag="VFX94_03195"
     CDS             complement(865..1521)
                     /locus_tag="VFX94_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5767216.1"
                     /translation="MREQLLARFPVFAGLNPASLERLLHEAQPVRAPKASVMFRPGQP
                     CRGFPLLLEGTVRVSQTSPSGREIVLYHVEPGQGCLLSGGCLLGHSEYGACGVAETDV
                     TLLSIPPALFHELLLQHEPFRRFVFAMYGERLAQVMQLVEEVAFRRLDTRLAQLLLQR
                     GPVVAATQQKIAEELGSVREIVSRLLRQFETRGWVALERERVTVRDRAALAELAGRED
                     "
     gene            1649..1855
                     /locus_tag="VFX94_03200"
     CDS             1649..1855
                     /locus_tag="VFX94_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEX5767217.1"
                     /translation="MSIEKVVRIFAGAVVLLSLALAVMVNLNWLWLTAFVGANLLQSG
                     FTGICPLESLLKRTPWFRSSARPA"
     gene            1825..>2188
                     /gene="trxC"
                     /locus_tag="VFX94_03205"
     CDS             1825..>2188
                     /gene="trxC"
                     /locus_tag="VFX94_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HEX5767218.1"
                     /translation="MVQVVCPSCLTANRVPDKRLRESPRCGKCRAPLLAGDPVALDDS
                     SFEALVGRTELPVVVDFWAPWCGPCRAMAPQFERAARELKGRARLVKVNTEEAPGLAS
                     RLGIRAIPTIALFRGGAEV"
BASE COUNT          368 a          777 c          715 g          328 t
ORIGIN      
        1 ggtgacagcc aggtgaatgc gccgtacagc gacggcgcga tcaccaggcc ggcgagaaac
       61 gcgaagcgcc agccgagatc gggcagcgcc gggcgaaaca gtccgccgat gatgccgctg
      121 atgccggcga cgcggccgtt cagcagcacg agcatcgcag ccgagatccc gatcagtacg
      181 cctccagcga gcgacgccca gggagtaaag tgagcccaat cgatgttcat gaaaaatcat
      241 cccgtggttc gagcaatcgt catggcggcc gcactcgcat cgtgcgccgc cgcagcgcag
      301 acgccgcaca cccaccggca cggcttcggc gacgcggaaa aatggtcgca cattttcgac
      361 gatccgaagc gcgacgcctg gcagaagccg cacgaggtca tcagcgcgct cgcgctcaag
      421 tccgacgccc tcgtcgccga catcggagcc ggcaccggct atttcagcgt gcgcctcgcc
      481 aatatgcacc cgagggccac cgtctatgcg gtggacgtcg aggccgacat ggtgcgctac
      541 ctcggcgagc gcgcgagccg tgagggcatc aagaacctga agccggtggc gggcctcgcc
      601 gaggatcccc gcctgccgga aaaggtcgat ctcgccctct tcgtggatgt ctaccaccac
      661 atcgagcggc gcgaggccta cttccgccgg ctggctggct cgctcaagcc cggcgcgcgc
      721 gtggcggtca tcgactttcg tctcgactcg cccgaagggc cgcccaaggc cgcgcgtgtc
      781 gcgcccgagc gcgtcaagaa ggagctcgcc gccgccggct acgcgctcga agccgagcac
      841 ggctttctcc cccatcagta tttcctagtc ttctcgcccc gctagctcgg cgagcgcggc
      901 gcggtcgcgc accgtgacgc gctcgcgctc gagcgccacc cagccacggg tctcgaactg
      961 gcgtaggagc cggctcacga tctcgcgcac gcttcccagc tcttcggcga tcttctgctg
     1021 cgtcgccgcc accaccggcc cgcgctggag gagaagctgc gccagccgcg tgtccaggcg
     1081 gcggaacgcg acctcctcga caagctgcat cacctgcgcc aggcgctcgc cgtacatcgc
     1141 gaacacgaag cgccggaaag gctcgtgctg gagcagcagc tcgtggaaga gcgccggcgg
     1201 gatactgaga agggtgacgt ccgtctcggc gacgccgcac gcgccgtact ccgagtgccc
     1261 aaggagacag cctccggaga gcaggcagcc ctgtcccggc tcgacgtggt agaggacgat
     1321 ttcgcgcccg ctcggcgagg tttgcgaaac ccgcaccgtg ccttcgagca gcagcggaaa
     1381 tccgcggcag ggctgccccg gacggaacat cacgctcgcc ttcggcgccc gcacgggctg
     1441 cgcctcgtgc aacagtcgtt cgagcgatgc cgggttaagg cccgcgaaca ccgggaagcg
     1501 tgcaagcagc tgctcgcgca tcgctcgagt ctagttgacc aggctcaagc ccggaaactc
     1561 cagccccggc actatggaac ctgggtcgca gaccgcagca gcgcggctcg ccacaatggc
     1621 gctactcgtt tcctccatgg agaatcgcat gagcatcgaa aaagtcgtcc gcatcttcgc
     1681 cggagcggtg gtccttctgt cgctcgcgct ggcggtcatg gtgaacctga actggctctg
     1741 gctgaccgcc ttcgtcggtg cgaacctttt gcagagcggc ttcaccggca tctgccctct
     1801 cgagtccctt ctcaagagaa cgccgtggtt caggtcgtct gcccgtcctg cctgaccgcc
     1861 aaccgggttc cggacaagcg cctgcgcgaa agcccgcgct gcggcaaatg ccgtgcgccc
     1921 ttgctcgcgg gtgatccggt tgcgctcgac gactcgagct tcgaggcgct cgtcgggcgc
     1981 accgagctgc cggtcgtggt cgatttctgg gcgccgtggt gcggcccgtg ccgcgcgatg
     2041 gcgccacagt tcgagcgcgc ggcgcgcgag ctcaaaggcc gcgcgcgcct ggtcaaggtc
     2101 aacaccgagg aagcgcccgg gctggcgtcg cgcctgggca tccgcgccat ccccacgatc
     2161 gcgctgttcc gcggcggcgc cgaagtga
//
LOCUS       DASUNT010000165         2681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_109957, whole genome shotgun sequence.
ACCESSION   DASUNT010000165 DASUNT010000000
VERSION     DASUNT010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109957"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..114
                     /locus_tag="VFX94_03210"
     CDS             7..114
                     /locus_tag="VFX94_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX5767219.1"
                     /translation="MYQGRIVERGSVDEVLRRPRHPYTQQLLAAVPTID"
     gene            114..1898
                     /locus_tag="VFX94_03215"
     CDS             114..1898
                     /locus_tag="VFX94_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5767220.1"
                     /translation="MRIYWLIFGALLSPELHAAHAYSQFGDIKYPAGFSHFEWVDPDA
                     PKGGEIELVPPLRITNFDKYNPFTLKGTAPPGTGLLFESLLTGTMDEPTTAYGLLAED
                     VEVASDGLSVTFRINPAARFHDGAPVTAADAKHSFDMLMSKQAAPQYRVIYGDIKQAV
                     VLGPRTIRYDFKRPNPELPLMAGGLPVFSRAWGAGKPFDQIVMDIPIASGPYKIGRLN
                     FGRDITYERDPSYWARELNVRRGRYNFDRVTYKIYKDTTAQTEAFKAGEFDYLRTFSA
                     REWARTYVGKKFDSGELVKVELATKNAGDFQGFLINTRRAKFKDPRVRQALGLAFDFE
                     WMNRQMMYNAYTRVRGFFNASDFEAKGLPSAEELEILSPLKKHIPEKVFNEDVPMPPS
                     TSPPGSLRDNLRKARDLLSEAGWTYRDGALRNGKGEPFTVEYLDSGGGERLVAPYFQA
                     LAKLGIAGEYRRADFALIRKRLDVFDYDIFTVRIPGSESPGAELLDRFGSQSADTEGS
                     SNLIGVKDPAVDALVNLAVSSRTRAELVARLRALDRVLRHNYYVIPQWYASTFRIAYR
                     AGKFAQPKVMPQYYQAEDWVISTWWRTP"
     gene            1899..>2681
                     /locus_tag="VFX94_03220"
     CDS             1899..>2681
                     /locus_tag="VFX94_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5767221.1"
                     /translation="MWSYILKRLLLMIPTLFGILLITFVVIQFVPGGPVEQMVAQFQG
                     FEAGGEGPAAAEGGYRGRQGVDAVRVEEIKKLYGFDKPAPERFVQMIGQFARFDLGKS
                     FFHNKAVGELILEKLPVSISLGLWTFLLVYLISVPLGIAKAVRAGTPFDTVTTFVVLL
                     GYAIPSFVMGVVLLVFFAGGSFFQWFPLRGITSLNWGEMNPIDKVLDYFWHIALPVGA
                     MVLGGFAVITILTKNSVLEEIRKHYVLTARAKGVDERRVLWRH"
BASE COUNT          468 a          870 c          870 g          473 t
ORIGIN      
        1 gcggtgatgt accagggcag gatcgtggag cgcggcagcg tggacgaagt gctgcgccgc
       61 ccgcggcatc cctataccca acagctcctc gccgccgtac cgaccatcga ctgatgcgaa
      121 tttactggct catcttcggc gcgctgctca gccccgagct gcacgcggcc cacgcgtatt
      181 cgcagttcgg cgacatcaag tatccggcgg gcttcagcca cttcgagtgg gtggaccccg
      241 acgcgccgaa aggcggcgag atcgagctcg tgccgccgct gcgcatcacg aacttcgaca
      301 agtacaaccc tttcacgttg aaaggcaccg cgccgcccgg cacgggactg ctgttcgaga
      361 gcctgctgac gggcaccatg gacgagccga ccacggctta cgggctactc gccgaggacg
      421 ttgaggtggc gagcgacggc ctgtcggtca cttttcgcat caatccggca gcgcgctttc
      481 acgacggcgc gccggtgacc gcggccgatg cgaagcacag cttcgacatg ctgatgagca
      541 agcaggccgc gccgcagtac cgggtgatct atggcgacat aaagcaggcc gtggtgcttg
      601 gtccgcgcac catccgctac gacttcaagc gtccgaaccc cgagcttccg ctcatggcgg
      661 gcggcctgcc ggtcttcagc cgcgcctggg gcgcgggcaa gccgttcgac cagatcgtga
      721 tggacatccc gattgccagc gggccgtaca agatcggccg gctcaatttc ggccgcgaca
      781 tcacctacga gcgcgatccc agctactggg cgcgcgagct gaacgtgcgc cgcggccgct
      841 acaacttcga ccgcgtcacc tacaagatct acaaggacac cacggcgcag accgaggcgt
      901 tcaaggcggg cgagttcgac tacctgcgca ccttctcggc gcgcgaatgg gcgcgcacct
      961 acgtcggcaa gaaattcgat tcgggcgagc tcgtcaaggt ggagcttgcg accaagaacg
     1021 cgggcgattt ccagggcttc ctcatcaaca cgcggcgtgc aaagttcaag gacccgcgcg
     1081 tgcgccaggc gctcgggctc gcgttcgact tcgagtggat gaaccggcag atgatgtaca
     1141 acgcctacac gcgcgtgcgc ggcttcttca acgcgagcga cttcgaggcg aaaggcttgc
     1201 cgagcgctga ggaactggaa attctgtctc ccctgaaaaa acacatcccc gagaaggtgt
     1261 tcaacgagga cgtgccaatg ccgccgtcga ccagcccgcc tggcagcctg cgcgacaacc
     1321 tgcgcaaggc gcgcgacctg ctgtccgagg cgggctggac gtaccgcgac ggcgccctgc
     1381 gcaacggcaa gggcgagcct ttcacggtcg aatacctcga cagcggcggc ggcgagcgcc
     1441 tggtggcgcc gtatttccag gcgctcgcca agctgggcat tgcgggcgag tatcgccgcg
     1501 ccgacttcgc gctcatccgc aagcggctcg atgtcttcga ctacgacatc ttcacggtgc
     1561 gcattccggg gagcgagtcg cccggagccg agctgctgga ccgcttcggc tcgcaatcgg
     1621 ccgataccga gggctcgagc aacctgatcg gcgtcaagga cccggcggtc gacgcgctcg
     1681 tgaacctcgc ggtgtcttcg cgcacgcgcg cggagctcgt tgcacggctg cgcgcgctgg
     1741 accgtgtgct gcgtcacaac tactacgtca tcccgcagtg gtatgcgagc accttccgca
     1801 tcgcgtaccg cgcgggaaaa ttcgcgcagc cgaaagtgat gcctcagtac taccaggccg
     1861 aggactgggt catatcgacg tggtggagaa cgccgtagat gtggtcgtac atcctcaagc
     1921 ggctgctcct catgatcccg acgctcttcg ggatcctgct catcaccttc gtcgtgatcc
     1981 agttcgttcc gggcgggccg gtggagcaga tggtggcgca gttccagggc ttcgaggcgg
     2041 gcggcgaggg gccggcggcg gcggagggcg gctaccgcgg ccgccagggc gtggacgcgg
     2101 tgcgtgtcga ggagatcaag aagctctacg gcttcgacaa gcccgcgccg gagcgcttcg
     2161 tgcagatgat cggccagttc gcccgcttcg atctcggcaa gagctttttc cacaacaagg
     2221 cggtcggcga gctgatcctg gaaaagctgc cggtgtcgat cagcctcggg ctgtggacct
     2281 tcctgctggt gtatctcatc tcggtgccgc tcggcatcgc caaggcggtg cgcgccggca
     2341 cgccgttcga caccgtgacg acgttcgtcg tgctgctggg ctacgcgatc ccgagcttcg
     2401 tcatgggcgt ggtgctgctt gttttcttcg ccggcggctc gttcttccag tggtttccgt
     2461 tgcgcggcat tacctccctc aattggggag aaatgaatcc aatcgacaag gttcttgact
     2521 acttctggca catagcgctt ccggttggcg ccatggtgct cggcggcttt gccgtgatca
     2581 ccatcctcac caagaactcg gtcctcgaag agattcgcaa gcattacgtg ctcacggcgc
     2641 gcgcaaaagg cgtcgacgag cggcgcgtgc tgtggcggca c
//
LOCUS       DASUNT010000166         3550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_197629, whole genome shotgun sequence.
ACCESSION   DASUNT010000166 DASUNT010000000
VERSION     DASUNT010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197629"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VFX94_03225"
     CDS             complement(<1..502)
                     /locus_tag="VFX94_03225"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX5767222.1"
                     /translation="MREAGVKEKGARKTIRIVPRTPLPKPAWIRVRAASPNSRFYEIK
                     HILREQKLHTVCEEASCPNIAECFGKGTATFMILGDICTRRCPFCDVAHGRPLAPDAE
                     EPRHLAETISRLGLKYVVITSVDRDDLRDGGAGHFVECIRAVRSLSPATRIEVLTPDF
                     RGRLDRA"
     gene            complement(495..1100)
                     /gene="lipB"
                     /locus_tag="VFX94_03230"
     CDS             complement(495..1100)
                     /gene="lipB"
                     /locus_tag="VFX94_03230"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX5767223.1"
                     /translation="MTVVDHIVVRRLGRADYFATWEAMRSFTRARDDSTPDEFWVLEH
                     PPVYTVGQGAPVPLIHSDIPVIKTDRGGDITYHGPGQVVIYTLVDLARRSIKVKRFVW
                     LLEQAVIDLLCGCAERKTGAPGVYVDGAKIAALGIRVVRGRAYHGVALNVDMELSPFS
                     AIDPCGYPGLRVSQMKDLGFAQTPATVASCLSQVLVKKLDA"
     gene            complement(1097..1309)
                     /locus_tag="VFX94_03235"
     CDS             complement(1097..1309)
                     /locus_tag="VFX94_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HEX5767224.1"
                     /translation="MGRKQPRFVQSVTGIVRKHAPDFDAATVEMRPSRQGKYLSVTCV
                     VRAISREQLDALYHELCDHPAVVMVL"
     gene            complement(1381..2253)
                     /locus_tag="VFX94_03240"
     CDS             complement(1381..2253)
                     /locus_tag="VFX94_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HEX5767225.1"
                     /translation="MTVFLSGRLLPIEQATVSVLDRGFVFGDGVYELVPVYSRVPFRL
                     EEHLARLERSLGETGIRNPYSRAQWRTHIYALIDAQPFEDQGVYFQVTRGVAKRDHAF
                     PANVEPTVFMMSNPLVNPQPAQVEKGASAVSAQDYRWLRCDIKSISLLGNCLLRQISA
                     EAGGAETILFREGKLTEASASNVFIVERGVIQSPPKSNLILPGITYDVVVELARANGL
                     PLEFKDIPEADVRAADELWVSSSSKEVLAIVELDGKRIGDGRPGPVFRRMFHLYQEFK
                     QKVMRAGKREAVSA"
     gene            complement(2250..3371)
                     /locus_tag="VFX94_03245"
     CDS             complement(2250..3371)
                     /locus_tag="VFX94_03245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX5767226.1"
                     /translation="MRFFLALVASASFGVQAAAPQPPSIIGKSWVLGDLSSGQVLAAQ
                     KAEERFEPASLTKMMTAYVVFAALRDRKLALDQQLKVSERAWKAPGARMYLEPRRPVV
                     VEELIRGMVVQSANDACVALAEALAGSEEAFAQMMNREAERLGMKNTRFVNASGLPHA
                     QHYSTAQDLYNLAAALIRDFPDEYAQYYSQKEFRYNNIPQVNRNRLLWIDPSIDGVKT
                     GQTEAAGYCLVASSRRGGRRLLSVLLGSTSEATRAQESLKLLNWGYQFFDSVKLYAAG
                     ATVRSLEVWKGASRSVEVGVGADLFVTVPKGEADRLKAELVSQQPLVAPLGKGQRVGV
                     LRVTHDGRPFAEYPLVTLEPVAMAGFPRRTWDTLRLWLK"
     gene            complement(3395..>3550)
                     /locus_tag="VFX94_03250"
     CDS             complement(3395..>3550)
                     /locus_tag="VFX94_03250"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlpA-like double-psi beta-barrel
                     domain-containing protein"
                     /protein_id="HEX5767227.1"
                     /translation="SVVVRINDRGPFRGTRIIDVSYVAAHKLGFIQAGHAQVDVEAVV
                     PGRTAGL"
BASE COUNT          638 a         1147 c         1173 g          592 t
ORIGIN      
        1 gcgcgcggtc gaggcgcccg cggaagtcgg gggtgaggac ctcgatgcgc gtggcggggg
       61 aaaggctgcg caccgcacgg atgcattcga cgaagtggcc ggcgccgccg tcgcgcaggt
      121 cgtcgcggtc gacgctggta atgaccacgt atttcaggcc gagacgcgag atcgtttcag
      181 cgagatgccg cggctcttcg gcgtcgggag cgagtggccg gccatgcgca acgtcgcaga
      241 acgggcagcg gcgcgtgcag atgtcgccta ggatcatgaa agttgccgtg cccttgccga
      301 agcactcggc aatattcggg cacgacgctt cttcgcacac agtgtgcagc ttttgttcac
      361 gcaggatgtg cttgatctcg tagaagcgcg agttcggact cgcggcgcgc acgcggatcc
      421 acgccggctt cggcagcggc gtgcgcggca cgatgcggat cgttttgcgg gcgccttttt
      481 cctttacgcc agcttcacgc atcgagcttt ttaaccagaa cttgagacaa gcaggaagcc
      541 acggttgccg gcgtctgggc aaaacccagg tccttcattt gcgacacacg cagccccgga
      601 tagccacagg gatcgatggc cgagaacggc gagagttcca tgtcgacatt gagcgccacg
      661 ccgtgataag cgcggccgcg cacgactcgg atgccgagcg ccgcgatctt cgccccgtcg
      721 acgtacacgc ccggcgcgcc ggtcttgcgc tcggcacacc cgcagagcag gtcgatcacc
      781 gcctgctcga gcaaccacac gaagcgcttg accttgatcg agcgccgtgc aaggtccaca
      841 agcgtgtaga tcacgacctg ccccgggccg tggtatgtga tatcaccgcc gcggtcggtc
      901 ttgatgaccg gaatgtcgct gtggattaac ggaaccggcg cgccctgccc caccgtatag
      961 accggcgggt gctcgagcac ccagaactcg tcgggagtcg agtcgtcgcg cgcccgggtg
     1021 aacgagcgca tggcttccca ggtcgcgaaa taatccgcgc ggccgagccg cctgacgacg
     1081 atatggtcga cgacggtcat aggaccatga ccacggcggg atggtcgcac agctcgtggt
     1141 agagggcgtc gagttgctcg cgcgagatgg cgcgcaccac gcaggtaacg ctcagatact
     1201 ttccctggcg gctcgggcgc atctcgactg tcgcggcgtc gaagtcaggt gcgtgcttgc
     1261 gcacgatgcc ggtgaccgac tgcacgaagc gcggctgctt gcggcccatg accttgatcg
     1321 gaaaatcgca aggaaacgct aaaggcgagc catccgctac gaccggcacg gccgagcctc
     1381 ttaagccgat acggcctcgc gcttgccggc gcgcatgacc ttctgcttga actcctgata
     1441 gaggtggaac atgcggcgga acaccgggcc ggggcgccca tcgccgatgc gcttgccgtc
     1501 gagctccacg atagcgagca cctccttgga cgaggagctg acccagagct cgtccgcggc
     1561 gcgcacgtcg gcttccggga tgtccttgaa ttccagcggc aggccgttcg cgcgcgcaag
     1621 ttcgaccacc acgtcgtatg tgatgccggg gaggatgagg ttcgacttcg gcggcgactg
     1681 gatcacgccc cgctccacga tgaaaacgtt cgaagccgag gcctcggtca gcttcccctc
     1741 gcggaagaga atcgtctccg ccccgcccgc ttcggcggaa atctgccgca gcaggcagtt
     1801 gccgaggagc gagatcgact tgatgtcgca gcggagccag cggtagtcct gcgcgctcac
     1861 cgccgaagcg cccttctcga cctgcgccgg ctgcgggttc accagagggt tggacatcat
     1921 gaacaccgtt ggttccacgt tggccggaaa tgcatgatcg cgcttcgcga cgccgcgggt
     1981 gacctggaag tagacgcctt ggtcctcgaa gggctgcgca tcgatcagcg cgtagatgtg
     2041 cgtacgccac tgcgcgcgcg agtacgggtt gcggatgccg gtctcgccca ggctgcgctc
     2101 caggcgcgcg aggtgctcct cgagccggaa aggcacgcgc gagtacaccg gcacgagctc
     2161 gtacacgccg tcgccgaaga cgaagccgcg gtcaaggacc gagaccgtcg cctgctcgat
     2221 cggcaggagc ctgccgctca gaaaaaccgt catttgagcc ataaccgcag tgtatcccag
     2281 gtgcgccggg ggaatcccgc catggccacc ggctcgagcg tcacgagcgg atattcggcg
     2341 aagggcctgc cgtcgtgcgt gacgcgcagc acgccgacgc gctggccctt gccgagcggc
     2401 gcgacgagcg gctgctggga aacgagctct gctttaagcc ggtccgcctc acccttcggc
     2461 acagtgacga agagatcggc gccgacaccg acctccacgc tgcgcgaggc gcctttccag
     2521 acctcgagcg agcgcaccgt ggcgccggcg gcgtagagct taacgctgtc gaaaaactga
     2581 tatccccaat tgaggagctt gagagactcc tgcgcgcgcg tcgcttccga ggttgagccg
     2641 agcagcaccg acagcaagcg ccgtccgccg cggcgcgacg aggcgacgag gcagtacccc
     2701 gcggcctcgg tctgtccagt cttcacgccg tcgatgctcg ggtcgatcca cagcagccga
     2761 ttgcggttca cctgcgggat gttgttgtag cgaaactcct tctgcgagta gtactgcgca
     2821 tactcgtcgg ggaaatcgcg gatcagcgcg gcggcgaggt tgtacaggtc ctgagccgtg
     2881 ctgtagtgct gggcgtgtgg cagccctgag gcattcacga accgggtgtt cttcatgccg
     2941 agccgctcgg cctcacggtt catcatctgg gcgaaagcct cctcgcttcc ggcaagcgcc
     3001 tcggcgagtg cgacgcaggc gtcgttggcc gactgcacca ccatgccgcg gatcagctct
     3061 tccaccacta ccggccggcg tggctccagg tacatccgag ctcccggggc cttccaggcg
     3121 cgctccgaca cctttaactg ctggtcgagc gcgagttttc tatcgcgcag tgcagcgaac
     3181 acgacgtacg ccgtcatcat cttcgtgagc gacgcgggct cgaagcgctc ctccgctttc
     3241 tgggcggcga gcacctgtcc gctcgagagg tcgccgagca cccaggactt gccgatgatc
     3301 gagggcggct gcggggcagc cgcttgaact ccaaaagaag cggaggcgac taaagcgaga
     3361 aaaaacctca tccgcgtgcc gaaaaagcgg cggattataa gcccgcggtt ctgccgggca
     3421 cgacggcctc cacgtcgacc tgcgcatgtc cagcctgaat gaagccgagc ttgtgcgcag
     3481 cgacgtacga gacgtcgatg atgcgggtgc cgcggaacgg gccgcggtcg ttgatgcgaa
     3541 cgactacgct
//
LOCUS       DASUNT010000167         3834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_460869, whole genome shotgun sequence.
ACCESSION   DASUNT010000167 DASUNT010000000
VERSION     DASUNT010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460869"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /gene="bamD"
                     /locus_tag="VFX94_03255"
     CDS             <1..250
                     /gene="bamD"
                     /locus_tag="VFX94_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX5767228.1"
                     /translation="MKRGAYLAAANRAQYAIKHYPQAPALEEAVAVLVLAYDRLGMND
                     LRDAADRVMRANFPESRYLKPGGFRKDVPWWRLWDPNW"
     gene            244..1197
                     /gene="denD"
                     /locus_tag="VFX94_03260"
     CDS             244..1197
                     /gene="denD"
                     /locus_tag="VFX94_03260"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HEX5767229.1"
                     /translation="MVSRVLIIGAAGMIGRKLVRRLGGEELILHDVVPFEGASVVSDL
                     SAPGEAEKLIASRPQLVFHLAAVVSGEAEADFDKGYRVNLDGTRALFEAIRKAGDGYK
                     PRVVFTSSIAVFGAPFPEAIGEEFLSAPLTSYGTQKAMCELLLSDYSRRGFFDGIGIR
                     LPTICVRPGKPNKAASGFFSGIIREPLAGQEALLPVPETVRHWFASPRAAIGFLLHAA
                     KIDTAQLDARRNLSMPGVSASVGEQIAALRRVAGDKVANLIRREPDPDIMRIVEGWPR
                     NFDAKRATGLGFRADSSFEHIIRAHIEDELQGSSAPRRAPA"
     gene            complement(1133..3013)
                     /locus_tag="VFX94_03265"
     CDS             complement(1133..3013)
                     /locus_tag="VFX94_03265"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX5767230.1"
                     /translation="MNGFRLRPQQVEMARAIHEAMTRTGVLVAEAGTGTGKTFAYLVP
                     ALLAGGKVIVSTGTKTLQDQLFDRDLPAVRAALASGASAALLKGRANYVCLYRLRRAS
                     AQAQLQSRDELLQLKRIERFAAVSATGDRADLPDVPEDAPVWAHATSTRENCLGQSCP
                     DFGDCFVMRARRNALAADVVVINHHLFFADVVLREEGISELLPACNTVVFDEAHQLAE
                     TARLFFGETVSSAQLVDLARDARAELRAAGGASPELEQLAARLDKAARDLRLVFGEAP
                     ARFAWSQALRMPGFADSLQMVQNALRSLHATLEVQAERSEGIAACARRAGTALAVLAR
                     MRESEPADEVRWVEVFAHAVRLHATPLSSAELFSRQMDDHPRAWIFTSATLAVGEEFG
                     HFTRELGLEQAALRRWPSPFDFARQALLYVPRGLPGDPNDPSYTDAVLDAAAPVLEAS
                     GGRAFLLFTTLRALRRAHERLRGMLPYPLLVQGTGSRSELLARFRELGNAVLLGAQSF
                     WEGVDVRGEALSVVVIDKLPFAPPDDPVLAARIEAIRARGGNPFTELQLPQAVLSLKQ
                     GAGRLIRDEADRGVLMLCDPRLAGRAYGRQILKSLPPMKLTRELAEVRSFLGARPRCG
                     RG"
     gene            complement(3064..>3834)
                     /locus_tag="VFX94_03270"
     CDS             complement(3064..>3834)
                     /locus_tag="VFX94_03270"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEX5767231.1"
                     /translation="GMHWFSWLRWLIAGTLAALAAVATGQHALEIIPLRHRTVEQVVP
                     VLQPLVEPGGTLSGSRGQLFVRASPANVAEIKRALEAIDRPARRLQISVRFDDTDDLQ
                     RRGVTGSATVGNAGARVGVSAEESRRQSTERVDQRLQVLEGSRAFIFSGQSRPLPLPG
                     GTTEIQDIESGFEVTPRLSGERVVLEVAAQRETAGARPGQVQGQRVASTVTTRLGEWV
                     EVGGTGSQEARDDRGIGTARTARVQQSRRVWLKVEELP"
BASE COUNT          592 a         1377 c         1307 g          558 t
ORIGIN      
        1 catgaagcgc ggcgcgtatc tcgccgcggc gaaccgcgcg cagtatgcga tcaagcacta
       61 tccgcaggcg cctgcgctgg aagaagcggt cgcggttctg gtgctcgcct acgaccgcct
      121 cggcatgaac gacctgcgcg atgccgccga ccgcgtcatg cgcgcaaact tccccgagag
      181 ccgctacctc aagcccggag gcttccgcaa ggacgttccg tggtggcggc tgtgggatcc
      241 gaactggtga gcagggttct catcatcggc gcggccggca tgatcggccg caagctcgtc
      301 cgccgcctgg gcggcgaaga gctcatcctg cacgacgtcg tgccgttcga aggtgcaagc
      361 gtcgtgtcgg acctatccgc gccaggcgaa gccgagaagc tcatcgcctc gcgcccgcag
      421 ctcgtctttc acctcgccgc ggtggtatcc ggcgaggccg aggcggactt cgacaagggc
      481 taccgcgtga acctcgacgg cacgcgagcg ctcttcgaag cgatccgcaa agcgggtgac
      541 ggatacaagc cgcgcgtggt ttttacttcg tccatcgccg tcttcggcgc gccctttccc
      601 gaggcgatcg gcgaggagtt cctgagcgct ccgctcacga gctacggcac gcagaaggcg
      661 atgtgcgagc tgcttctttc cgattacagc cgccgcggct tcttcgacgg catcggcatc
      721 cgcctgccga cgatctgcgt gcggccgggc aagccgaaca aggccgcctc cggattcttc
      781 tccggcatca tccgcgagcc gcttgccggg caggaagcgc tgctcccggt ccccgagacg
      841 gtgcgccact ggttcgcgtc gccgcgcgcg gcgatcggtt tcctgctcca cgccgcgaaa
      901 atcgacaccg cgcagctcga tgcgcggcgc aatctcagca tgccgggagt tagcgcgagc
      961 gtcggcgagc agatcgcggc gctgcgcagg gtggccggcg acaaggtcgc gaacctgatc
     1021 cgccgcgagc ccgaccccga catcatgcgc atcgtcgaag gctggccgcg caacttcgac
     1081 gcgaagcgcg cgaccggact cggctttcgg gccgactcga gcttcgagca catcatccgc
     1141 gcccacatcg aggacgagct ccaaggaagc tccgcacctc ggcgagctcc cgcgtaagct
     1201 tcatcggcgg caggctcttc aggatctgcc ggccgtacgc cctgcccgcg agccgcgggt
     1261 cgcacagcat gagaaccccc cggtcggctt cgtcgcggat cagccgcccg gcgccctgct
     1321 tcaggctgag caccgcctgg ggcagctgca gctcggtgaa cggattgccg ccgcgcgcgc
     1381 gaatggcctc gatgcgtgcc gcgagcaccg gatcatccgg cggcgcgaac ggcagcttgt
     1441 cgatcacgac gaccgaaagc gcttcaccgc gcacgtcgac cccctcccag aacgactgcg
     1501 ctccaagcag caccgcgttg ccgagctcgc ggaagcgcgc cagaagctcg ctgcgcgagc
     1561 ctgtgccctg cacgagaagc gggtacggca gcatgccgcg cagccgctcg tgcgcgcgcc
     1621 gcaatgcgcg cagcgtagtg aaaagcagga acgcgcgccc gccgctcgct tcgagcaccg
     1681 gcgcggcggc atcgagcacc gcgtcggtgt aggaaggatc gttcgggtcg ccgggcaggc
     1741 cgcgcggcac gtacagcagc gcctggcgcg cgaagtcgaa tgggctcggc cagcgccgca
     1801 gcgccgcctg ctcgagaccg agctcgcgtg tgaagtgccc gaactcttcg ccgaccgcca
     1861 gcgtcgccga ggtgaatatc caggcgcgcg gatgatcgtc catctggcgg ctgaaaagct
     1921 ctgccgatga aagcggcgtg gcgtgcagtc gcaccgcgtg cgcgaacacc tcaacccagc
     1981 gcacctcgtc cgccggctcg ctttcgcgca tgcgcgcaag cacggcgagc gcggtgcccg
     2041 cgcgccgcgc gcaggccgcg atgccttcgg agcgctcggc ctgtacttcc agcgtggcgt
     2101 gcagggagcg caatgcgttc tgcaccatct gcaggctgtc ggcgaaaccg ggcatgcgca
     2161 gcgcctggct ccaggcgaag cgggccgggg cttcgccgaa cacgagccgc aggtcgcgcg
     2221 ccgccttgtc gaggcgcgcg gcgagctgct cgagctctgg cgaggcgccg ccggcggcgc
     2281 ggagctccgc gcgcgcatcg cgcgcgaggt ccacgagctg cgcgctcgac acggtctcgc
     2341 cgaagaaaag gcgcgcggtc tcggcaagct ggtgcgcctc gtcgaatacg acagtgttgc
     2401 aggccggcag gagctccgag atcccttcct cgcgcagcac cacgtcggcg aagaaaaggt
     2461 gatggttgat caccacgacg tccgcggcga gcgcgttgcg ccgtgcgcgc atgacaaagc
     2521 agtcgccgaa atcggggcac gactgcccca ggcagttttc gcgcgtcgag gtggcgtgcg
     2581 cccacaccgg cgcgtcttct ggcacgtcgg gcaggtcggc ccggtcgccc gtcgccgaca
     2641 cggcggcaaa gcgctctatg cgcttcagct gaagcagctc atccctggat tggagctggg
     2701 cctgcgcgct cgcgcggcgc agacggtaaa ggcaaacgta gttcgccctg cccttgagga
     2761 gcgcggcgct cgcgccgctc gcgagcgcgg cgcgcaccgc gggaaggtcg cgatcgaaga
     2821 gctggtcctg cagggtcttg gtgccggtcg agacgatcac cttgccgccg gcgaggagcg
     2881 ccggtacgag ataggcgaac gtcttgccgg tgccggtgcc ggcctcggcc acgagcacgc
     2941 ccgtccgggt catggcctcg tggatggccc gcgccatctc gacctgctgc ggccgcagcc
     3001 tgaatccgtt cactgtagag gcgagcgcac cgtcgacggc gaatgcccgc tccagcgcgc
     3061 cgttcagggt agctcctcga ctttcagcca cacgcggcgc gattgctgga cgcgcgcggt
     3121 gcgcgccgtg ccaatgcctc ggtcgtcgcg cgcttcctgc gagccggtgc cgcctacttc
     3181 gacccattcg ccgagccgcg tggtgacggt gcttgcaacg cgctggccct gcacctggcc
     3241 ggggcgcgcg cctgccgtct cgcgctgcgc agcaacctcg agcacgactc gctcgcccga
     3301 gagccgcggc gtcacttcga accccgactc gatgtcctgg atctccgtcg ttccgcccgg
     3361 cagcggcagc ggccgggact ggccggagaa gatgaacgcg cggctgccct ccagcacctg
     3421 caggcgctgg tcgacgcgct cggtcgactg gcggcgcgac tcctcggcgc tcaccccgac
     3481 tcgggcgccc gcgttgccga cggtagcgct tccggtaacg ccccgccgct gcaggtcatc
     3541 ggtatcgtcg aagcgcaccg agatctggag ccgccgcgcc gggcggtcga tcgcctcgag
     3601 cgcgcgcttg atctcggcga cgttggccgg gctcgcgcgt acgaaaagct gtccgcggct
     3661 gccactcaac gtcccgccgg gctcaacgag cggctgcagt accggcacca cctgctccac
     3721 cgtgcgatgg cgcagcggaa tgatctccag cgcatgctgc cccgttgcaa cagcagcgag
     3781 cgcggccaac gtgccggcga tgagccaacg cagccaagag aaccaatgca tgcc
//
LOCUS       DASUNT010000168         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_285530, whole genome shotgun sequence.
ACCESSION   DASUNT010000168 DASUNT010000000
VERSION     DASUNT010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285530"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VFX94_03275"
     CDS             complement(<1..1175)
                     /locus_tag="VFX94_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767232.1"
                     /translation="MGSEPLPNAFARGYVAQSVRVRAQRAGGAEVPVSAVPDQDVVAL
                     HLANGPTLYLHPENARDLLRAQVAEQPRRAQDADPNRVTVLPQLSWDRLEQAGATRGA
                     ARGLGDVLLSLVEVIREPAADLVAGAVVKRVDEQVNEGVYALAVDGLTKLKEREPIDR
                     IPDSGDKPVLVFIHGTFSTTKGTFDKLWVQHPQRVQQLFRHYENRVYALEHRTLGQSP
                     IGNALTLAKVLPRNTPVHLVTHSRGGLVAEVLARACAPGVVTPKELELHFPGADHEPQ
                     RRQLSELARQLKDVRLKRILRVGCPARGTLLASKRLDAYLSVFKWALQLAGLPVVPEI
                     LDFLASVAQRRADWNVIPGLAAQLPDGVLVQWLHAPTEDRIPGELRVVAGDIAGDSVV
                     "
     gene            1371..>2083
                     /locus_tag="VFX94_03280"
     CDS             1371..>2083
                     /locus_tag="VFX94_03280"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX5767233.1"
                     /translation="MKSTIFLSYASSLDETAARIELSLKGEGYAVFRDRSALPPGESF
                     DARIRAAIEESELFVFLISPDSVTPGRYTLTELKFAEQKWAHPAGRVLPVLAEATPHE
                     AIPAFLRAVSILKPRGNLVAEVAAEVARLTAPWWRRMLEPRRLVPALVAALLVAGGAW
                     LALPPYLKQRQQAAEAAGIVERSRARAQAGEYAQAWKLLEQANAVAPGSRAVFDAQEQ
                     FAMERLRSAGLTYWSATAG"
BASE COUNT          318 a          725 c          724 g          316 t
ORIGIN      
        1 acgaccgagt cgccggcaat gtccccggcc acgacgcgaa gctcgccggg gatgcggtcc
       61 tcggtcggcg cgtgtagcca ttgcaccagc acgccgtcgg ggagttgcgc ggcaagcccc
      121 gggatcacgt tccagtcggc acgccgctgc gccaccgacg cgaggaagtc aaggatttcc
      181 ggcaccaccg gcaggccggc gagctgcaac gcccacttga acaccgaaag ataggcgtcg
      241 agccgtttgg aggcgagcag cgtgccgcgc gccggacagc cgacgcgcaa tatgcgcttc
      301 agccgtacat ccttcagctg ccgagcgagc tcggacagct ggcgccgctg cggttcatgg
      361 tctgcgccgg ggaaatgaag ttcaagctct ttcggggtca ccacgccggg cgcgcaggcg
      421 cgcgccagga cctccgccac caggccgccg cgcgagtgcg tgacgaggtg gaccggggtg
      481 tttctgggca gcactttcgc gagcgtgagc gcatttccta tcgggctttg gcccagcgtg
      541 cggtgctcga gcgcgtagac ccggttctcg tagtgccgga agagctgctg cacccgttgc
      601 gggtgctgca cccacagttt gtcgaacgtt cccttggtgg tggagaaagt gccgtggatg
      661 aagaccagga ccggcttgtc cccggaatcc gggatgcggt cgatcggctc acgctccttg
      721 agcttcgtca gtccatcgac ggcaagcgca tagacgcctt cgttgacctg ctcgtcgacg
      781 cgcttcacca ccgcgcccgc cacgaggtcc gcggccggct cgcggatgac ttccaccagc
      841 gacagcagca cgtcgcccaa tccgcgcgcc gccccgcggg tcgcgcccgc ctgctcgagg
      901 cgatcccagg agagctgggg gagcaccgtg acgcgattcg gatccgcatc ctgcgcgcgc
      961 cgcggctgct cggcgacttg cgcccgcagc aggtcgcgcg cgttttcagg atggaggtac
     1021 agcgtcggcc cgttcgccag gtgcagcgcc accacgtcct gatccggcac ggcgctaacc
     1081 ggtacctcgg ctccaccggc gcgctgcgcg cgcacccgga ccgactgcgc gacatacccg
     1141 cgcgcaaatg cgttcggcag cggctcggag cccatgcttc gctcagcgcc gcgcaactgc
     1201 gattggtctt gtcccgggac gacaaaagtg atgggcgttg ccatggatcg cctttcttgc
     1261 ttcggcgatt atgcagcgca cggcgaaaaa gacggatcgc aaaatcgagc ctgctcgccc
     1321 cagagcgggt aagctctcgg gcggcgaagc acgcgcccag ggctcgcccg atgaagagca
     1381 ccatcttcct ttcctacgcg tcctcgctcg atgagacggc cgcgcgcatc gagctctcgc
     1441 tcaaggggga gggctacgcg gtgttccgcg accgcagcgc gctgccgccg ggtgagtcct
     1501 tcgacgcgcg gatacgcgcc gccatcgagg agagcgagct cttcgtcttc ctcatctcgc
     1561 cggattcggt aacgccgggc cgctacacgc tcaccgagct caagttcgcc gagcagaagt
     1621 gggcgcaccc ggccgggcgc gtgctcccgg tgctcgccga ggcgacgccg cacgaggcca
     1681 ttccggcgtt cctgcgggcg gtgagcatcc tcaagccgcg cggcaacctc gtggccgagg
     1741 tcgccgccga agtcgcgcgc ctgaccgcac cgtggtggcg gcgaatgctc gagccacggc
     1801 ggctcgtgcc tgcgctcgtg gccgcgctac ttgtcgccgg cggcgcatgg ctcgcgctgc
     1861 cgccgtatct caagcagcgc cagcaggcag ccgaggcggc ggggatcgtg gagcgcagcc
     1921 gcgcgcgggc gcaggccggc gagtacgcgc aggcgtggaa gctgctggag caggcgaacg
     1981 cggtggcgcc cgggtcgcgg gcggtgttcg acgcgcagga gcagttcgcc atggagcgac
     2041 tgcgtagcgc cggcctcacc tactggagcg ccacggccgg cta
//
LOCUS       DASUNT010000169         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_22098, whole genome shotgun sequence.
ACCESSION   DASUNT010000169 DASUNT010000000
VERSION     DASUNT010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22098"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VFX94_03285"
     CDS             <1..662
                     /locus_tag="VFX94_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX5767234.1"
                     /translation="DMLFATAGVHPHHARGCTPQTIADLRELAKHPRCVAIGECGLDF
                     NRNYSPHPDQEKWFVAQIELGIELGKPLFLHSRDAHPRFSDILKEHEVRKAVAHCFTG
                     ERDELKAYIDLGLYIGITGWICDERRGKHLLELVKAIPKDRLLLETDAPYLTPRDLRP
                     QPKARRNEPAFLPHILRTVARALGRPADEVAAETVQNARELFSLHESGSSAAAKRPLA
                     A"
     gene            616..2070
                     /locus_tag="VFX94_03290"
     CDS             616..2070
                     /locus_tag="VFX94_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX5767235.1"
                     /translation="MNLVHLLLRSARWLPEQPALALGKRPVRTYREMASRVARMSSAF
                     RQKHRLAPGDRVALAMKNGPEFYEVLFACWHAGLTAVPMNAKLHPKEFAYILENSGAK
                     LCFVTPDLQAAVPGGIAVEEAASFLDEAAAPADVGPDDVAWIFYTSGTTGVPKGAMLT
                     HRNMLFQAQAYFADIDKLYPGDTALHPAPLSHGSGLYGLPHFAAGAVNVIPASGHFEP
                     AEVFDLLDYWPSCSFFAAPTMLVRLMASPAARTPQKLKTITYGGAPMYVADCLRAIEL
                     FGPRLFQLFGQGEAPMTITGLSQIFHQRREHLESCGLPRTGCEVKIFDRDDRELPAGE
                     VGEIVTRSDCVMKGYWQNPEASARALRGGWLRTGDVGSMDAQGFLTIKDRSKDMIISG
                     GANIYPREIEEVLLRHPAVAECSVVGRPHPEWGEEVVAFVVRRSGLGSDELDSLCLQN
                     IARFKRPREYRFVEALPKNNYGKVLKTELRKLLA"
     gene            complement(2110..>2462)
                     /locus_tag="VFX94_03295"
     CDS             complement(2110..>2462)
                     /locus_tag="VFX94_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX5767236.1"
                     /translation="SALVQRVYSVEIVAELAQQARQRLARQGCANVELRMGNGYYGWP
                     EHGPFDKILVCAGAELVPAPLLQQLRPGGRMVVPTGLPDSQQLMLVEKDPGGRTRMKE
                     TLRVRFSLLEGEPA"
BASE COUNT          422 a          834 c          786 g          420 t
ORIGIN      
        1 ccgacatgct tttcgccacg gcaggggtgc acccgcacca cgcgcgcgga tgcacaccgc
       61 aaaccatcgc cgacttgcgc gagcttgcga agcacccgcg ttgcgtcgcg atcggcgaat
      121 gcgggctcga cttcaaccgc aactactcac cgcatccgga ccaggaaaag tggttcgtgg
      181 cccagatcga gctcggcatc gagctcggga agccgctttt cctccattca cgcgatgcgc
      241 acccgcggtt ttccgacata ttgaaagagc acgaggtgcg gaaagcagtc gcgcattgct
      301 tcaccgggga acgtgacgaa ctcaaggcgt acattgatct cggtctgtac atcggtatta
      361 ccggctggat ttgcgatgaa cgccgcggca agcacctgct cgagctggtg aaggcgatcc
      421 cgaaagaccg cctgctcctc gaaaccgacg cgccctatct cacgccgcgc gacctgcggc
      481 cgcagcccaa ggcgcggcgc aacgagcctg cttttctccc gcatatcctg cgcaccgtgg
      541 cgcgcgcgct cgggcgcccg gccgacgaag tcgccgccga gacggtgcag aacgcaagag
      601 agctcttttc gctgcatgaa tctggttcat ctgctgctgc gaagcgcccg ctggctgcct
      661 gagcagcccg cactcgcgct cggcaagcgc ccggtgcgca cgtaccgcga aatggcctcc
      721 cgtgtggccc gcatgtcttc ggcttttaga caaaagcatc gtctcgcgcc aggtgatcgg
      781 gttgcattgg caatgaagaa cggcccggag ttctacgagg tgctttttgc ctgctggcat
      841 gccggcctga ctgcggtgcc gatgaacgcg aagctgcatc cgaaggagtt cgcttacatc
      901 ctcgaaaact cgggcgcgaa gctctgcttc gtcacgccgg atctccaggc cgcggtcccg
      961 ggcggaattg cggtcgagga agcggcgagc ttcttagacg aggcggccgc gcccgccgat
     1021 gtcgggcccg acgacgtggc gtggatcttc tatacgagcg gcaccaccgg cgtgccgaag
     1081 ggcgcgatgc tcacccatcg caacatgctt ttccaggcgc aggcctactt cgccgacatc
     1141 gacaagcttt acccgggcga cacggcgctg catcctgcgc cgctttcgca cggctcgggc
     1201 ctctacggcc tgccgcactt cgccgccggc gcggtgaatg tcatacccgc gagcggccac
     1261 ttcgagccgg ccgaggtttt cgacctgctc gactactggc cgagctgctc ctttttcgcc
     1321 gcgccgacga tgctcgtgcg cctgatggcg agccccgctg cgcgcacgcc gcagaagtta
     1381 aagaccatca cctacggcgg cgcgccgatg tacgtcgcgg actgcctgcg cgcgattgag
     1441 ctcttcggcc cgcgcctttt ccagctcttc ggccagggcg aggccccgat gacgatcacc
     1501 ggcctgtcgc agatctttca tcaaagaagg gagcacctgg aaagctgcgg gctgccgcgc
     1561 accggctgcg aagtgaagat cttcgatcgg gacgaccgcg agctgccagc gggtgaagtc
     1621 ggcgagatcg tcacgcgctc cgactgcgtg atgaagggct attggcagaa tccggaggcg
     1681 agcgcgaggg cgttgcgcgg cggctggctg cgcaccggcg atgtcggctc catggacgcg
     1741 caaggcttcc tcaccatcaa ggaccgctcc aaggacatga tcatctcggg cggcgccaac
     1801 atctatccgc gcgaaatcga ggaagtgctg ctgcgccatc ccgcggtggc cgagtgctcg
     1861 gtcgtcggtc gcccgcatcc cgagtggggc gaggaagtgg tcgcttttgt cgtcagaaga
     1921 tccggtctgg gcagcgacga gctggactcg ctttgcctcc aaaacatcgc ccgcttcaaa
     1981 cgcccccgcg agtaccgctt cgtcgaggcg ctgccgaaga acaattacgg caaggtgctg
     2041 aagaccgagc tgcggaagct gctcgcctag gatccccgcc tgcgcgggga cgacgatcca
     2101 acctttgctc taggccggct cgccttcgag aagcgagaag cgcacgcgca gggtttcctt
     2161 catccgggtg cgcccgcccg ggtccttctc caccagcatg agctgctgcg agtccggcag
     2221 gccggtcggg acgaccatgc ggccgccagg cctgagctgc tgcaggagcg gcgccggcac
     2281 gagctccgcg ccggcgcaca cgaggatctt gtcgaacggg ccgtgctcgg gccagccgta
     2341 gtagccgttt cccatgcgca gctccacgtt ggcgcagccc tggcgcgcaa gccgctgccg
     2401 cgcctgctgc gccagctcgg cgacgatctc cacgctgtat acgcgctgca cgagggccga
     2461 ga
//
LOCUS       DASUNT010000170         6821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1162764, whole genome shotgun sequence.
ACCESSION   DASUNT010000170 DASUNT010000000
VERSION     DASUNT010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162764"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..113
                     /locus_tag="VFX94_03300"
     CDS             <1..113
                     /locus_tag="VFX94_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5767237.1"
                     /translation="FKDPKYRPAPLLKEMVAAGKLGRKSGRGFHTYEATK"
     gene            110..301
                     /locus_tag="VFX94_03305"
     CDS             110..301
                     /locus_tag="VFX94_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767238.1"
                     /translation="MNRAYRKLILALAVALIAGCSTVTKPPPPAADAIQTRADTPLKA
                     RFSTEPVTGRNWNCPKCYS"
     gene            315..2612
                     /locus_tag="VFX94_03310"
     CDS             315..2612
                     /locus_tag="VFX94_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HEX5767239.1"
                     /translation="MDLGQKLSRSVDISERITRVLQQRLQAATERFRSRVEAATKGLQ
                     PAAAWWAWPQYAVDCAQRAVIFWDTLRQRGNNYLEHVQQGQPPVLHFKYETVLDGRTL
                     ERPVNYALLRIIPPEGVTVDANKRPYIIIDPRGGHGPGIGGFKDDSQVGVALRAGHPV
                     YFVIFYRDPEPGQTLLDVCAAEREFVHKVRALHPGSAKPAIVGNCQGGWAAMMLAASE
                     PGETGPLVIVGAPMSYWGGAWREGEGDNPMRYAGGILGGTWLASLCADLGNGTFDGAH
                     LVQNFEYLNPANTFVDKYYKVFANAETEPARFLEFERWWGGYFLMNREEIEWITRNLF
                     VGNKLWSGEVKSGPRGSGGFDLREIRSPIILFASLGDNITPPQQAFNWVADVYGSTEE
                     IKARGQVIVGLMHEEVGHLGIFVSGKVAQKEYTQIVSVMKSIEMLRPGLYGMEIHERK
                     ADGSVDYEVKFHEFRLEDVAARLNRHQRLDEKPFEAVAAVSEFNQRAYEIFGQPLVQA
                     SSNEFLATLMRRFHPLRMQRWALSDFNPWLAWLPGAAQLVKDKRQALAADHPLRRAEA
                     SLADLASATLEYHRALRDAFTEAAFFHIYGNLFSLYIADKQKALAPAAQLIEPRELPV
                     VKEALASIDKGGYPEAFARVGFLVTRRGDVPLSRLEAGQQMVKDYEEFLPAVPLEQMR
                     RMAGEQEIIATYEPDKAIATLPVLLPRREERERLLTLLERLENDERVRHDGLTAEQRA
                     MLERVRQALGAPMRARRVLPLKERA"
     gene            2615..4387
                     /locus_tag="VFX94_03315"
     CDS             2615..4387
                     /locus_tag="VFX94_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5767240.1"
                     /translation="MDLSLPRLPAPAAVPPAGNDIDRRIRAGLGRATGSLSVGSALLA
                     AGDWAINLLVSPGKQMELALLALDYAGQLARYDIECFLAGPGETRRHIKPPARDRRFD
                     GSEWLHWPFNVLHQSFLLTEQWWAAATRGVLGVERHHEELVSFAARQWLDLASPSNML
                     ATNPVALKKTLQECGQNLVRGATNALTDLQRMAAGLPPPGAEKFVVGANVAVTPGKVV
                     LRNRLIELIQYSPATPQVCPEPVLLVPAWIMKYYILDLSPANSLVKYLVDQGHTVFCI
                     SWKNPTVDERDLGMDDYLQLGLGAALDAVQAIVPQRKVHALGYCLGGTLLSIGAAAMA
                     RDKDDRLASLTLLAAQTEFSEPGELGLFIDESQVSLLEAQMAQTGYLSSQQMGGAFQL
                     LRSNDLLWSRLVNQWLLGEPSEVNDLMAWNADATRLPARMHSEYLRQLFLHDDLAESR
                     YRVGGRLVSLADIKAPIFLVGTVTDHVAPWRSVYKLHHLTPAEITFVLTSGGHNAGIV
                     SPPGHPRRHYQLLTSPAFTGQTDGRTDPDHWLAQAPEHQGSWWPAWHEWLRAHSGDPV
                     KPPRTGLPGERVLGDAPGRYVLEK"
     gene            4482..5240
                     /gene="fabI"
                     /locus_tag="VFX94_03320"
     CDS             4482..5240
                     /gene="fabI"
                     /locus_tag="VFX94_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX5767241.1"
                     /translation="MLKGKRGLVIGVANADSIAYGCAVKLRAFGADIALTYLNDKAKQ
                     YVAPLAEQIDASLLLPLDVTKPGQMQAVFERIRADWGRLDFVIHSIAFAPREDLHGRV
                     VDCSQAGFLQAMQVSCHSFLEMARCAEPLMSEGGSLITMSYYGADKVVNNYNVMGPVK
                     AALEASVRYLAKELGPRNIRVFAVSPGPLRTRASSGIAHFDELIDMAVKRSPGQRLVD
                     IAEIGRVVAFLAMPASSAMTGDVVYIDAGLHNMA"
     gene            5254..6714
                     /locus_tag="VFX94_03325"
     CDS             5254..6714
                     /locus_tag="VFX94_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HEX5767242.1"
                     /translation="MNSTFASEDLEVLRNRTFDEIKVGERASIERTLSASDIQLFAAM
                     SGDINPQHIDAEFAASTRFQGVIAHGMWGAALISAVLGTRLPGPGTIYAGQTLKFHAP
                     VRVGDTLTISVVVTERDEAKHRLVLDCNCTNQEGKTVISGQATVIAPTERIERPRGTL
                     PQVRVSLQHGDELVRVLQYVKPLGRIRVAVVHPCDEVSLSAALDARSAGVIEPVLIGP
                     RARLEAVAQKCGANLRDVAIEDVPHSHAAATRAVELALKGEVEALMKGSLHTDELMSA
                     VVKSALRTERRMSHCFIMQTPAYPRPFIITDAAINIAPTLQDKADIIRNAVDLAHAFG
                     IARPRVAILAAVETVNPNMPATLDAAALCKMADRGQIAGAILDGPLAFDNAVSAAAAR
                     TKGIVSEVAGAADILVVPDLEAGNMLAKQLEYLGGAASAGIVLGARVPIVLTSRADAR
                     EARIASCAVAVVVARRMGQPAVAIAAPRKQAAEPVQ"
BASE COUNT         1129 a         2394 c         2281 g         1017 t
ORIGIN      
        1 acttcaagga tcccaagtac cggccggcgc cgcttctgaa ggagatggtg gcggccggca
       61 agctcggccg caagtccggc cgcggattcc atacctatga ggccaccaaa tgaaccgtgc
      121 ataccgcaaa ctgatcctcg cgctggcggt cgcgctgatc gccggctgca gcaccgtgac
      181 gaagcctccc ccgccagccg cggacgccat acaaacccgc gccgacacgc cgctgaaggc
      241 gcgtttctcc accgaaccgg ttacggggcg gaactggaat tgtccgaagt gctactcgtg
      301 agcgcgcgtt caacatggac ctaggccaaa agctttcccg cagcgtcgac atttccgaac
      361 gcatcacccg cgtgctgcaa cagcgcctgc aggccgcgac cgagcggttt cgcagccgcg
      421 tggaggcggc aacgaagggc ttgcagcccg cggccgcgtg gtgggcgtgg ccgcagtacg
      481 cggtcgactg cgcgcagcgc gcggtgatct tctgggacac gctgcgccag cgcggcaata
      541 actatctcga gcatgtgcag cagggccagc cgccggtcct gcacttcaag tacgagacgg
      601 tgctcgacgg gcgcaccctc gagcggccgg tcaactacgc gctgctgcgc atcatcccgc
      661 ccgagggcgt gacggtcgac gcgaacaagc gcccctacat catcatcgac ccgcgcggcg
      721 gccacggccc cggcatcggc ggcttcaagg acgactccca ggtcggcgtc gcgctgcgcg
      781 cgggacaccc cgtctatttc gtcatcttct accgcgaccc cgagccggga cagacgctgc
      841 tcgacgtgtg tgccgcggag cgcgagttcg tgcacaaggt gcgcgcgctg catccgggca
      901 gcgccaagcc cgcgatcgtc ggcaactgcc agggcggctg ggcggcgatg atgctcgcgg
      961 cctccgagcc cggcgagacc gggccgctcg tgatcgtcgg cgcgcccatg tcttactggg
     1021 gcggggcgtg gcgcgagggc gagggcgaca acccgatgcg ctacgcgggc ggcatcctcg
     1081 gcggcacgtg gctcgcctcg ctttgcgcgg atctcggcaa cggcacattc gacggcgcgc
     1141 acctcgtgca gaacttcgag tacctcaatc ccgccaacac cttcgtcgac aagtactaca
     1201 aggtgttcgc gaacgccgaa accgagcccg cgcgcttcct cgagttcgag cgctggtggg
     1261 gcggctactt cctgatgaac cgcgaggaga tcgagtggat cacgcgcaat ctcttcgtcg
     1321 gcaacaagct ctggtccggc gaggtgaagt ccgggccgcg cggcagcggg ggcttcgacc
     1381 tgcgcgagat ccgctcgccg atcattctgt tcgcatcgct cggcgacaac atcacgccgc
     1441 cgcagcaggc gttcaactgg gtggccgacg tgtacggctc gaccgaggaa atcaaggcgc
     1501 gcggccaggt gatcgtcggc ctgatgcacg aggaagtcgg ccatctcggc attttcgtct
     1561 ccgggaaagt ggcgcagaag gagtacacgc agatcgtgtc cgtgatgaag tcgatcgaga
     1621 tgctgaggcc gggcctctac ggcatggaga tccacgagcg caaggccgac ggcagcgtgg
     1681 actatgaagt gaagttccac gagttccgcc tggaagacgt cgcggcgcgg ctgaaccgcc
     1741 accagcgcct cgacgagaag cccttcgagg ccgtcgcagc cgtctcggaa ttcaaccagc
     1801 gcgcctacga gatcttcggg cagcccctgg tgcaggcgag ctccaacgaa ttcctcgcga
     1861 ccctgatgcg ccggttccat cccctgcgca tgcagcgctg ggcgctctcc gatttcaacc
     1921 cctggctggc gtggctcccg ggcgccgccc agctcgtgaa ggacaagcgc caggcgctgg
     1981 ccgccgacca tccattgcgc cgcgcggagg cgagcctcgc ggacctcgcg agcgccacgc
     2041 tcgagtatca ccgggcgctg cgcgacgcat tcaccgaggc ggcgttcttc catatctacg
     2101 gcaacctgtt ctcgctctat atcgccgaca agcagaaggc gctggcgccc gcggcgcagc
     2161 tcatcgagcc gcgcgagctg ccggtggtca aggaagcgct cgcgtcgatc gacaagggcg
     2221 gctaccccga ggcctttgcc cgcgtcggtt tcctcgtcac gcgccgcggc gacgtgccgc
     2281 tctcgcgcct ggaagccggg cagcagatgg tgaaggacta cgaggaattc ctgcccgccg
     2341 tgcctctcga gcagatgcgc cgcatggcgg gcgagcagga aatcatcgcg acctacgagc
     2401 ccgacaaggc gatcgcgacg ctgccggtcc ttcttccgcg gcgtgaagag cgcgagcggc
     2461 tgctcacgct gctcgagcgg ctggagaacg acgagcgcgt ccggcacgac ggcttgacgg
     2521 cggagcagcg cgcgatgctc gagcgggtcc gccaggcgct cggcgcgccg atgcgcgcgc
     2581 ggcgtgtcct gcccctgaaa gaaagggctt aaccatggat ctctcgctac cgcgcctgcc
     2641 ggctccggcg gccgtgccgc ctgccggcaa tgacatcgac cgcaggattc gtgcagggct
     2701 cggacgcgcc accggcagcc tctcggtcgg ctcggcgctg ctcgccgccg gggactgggc
     2761 gatcaacctg ctcgtctcgc caggcaaaca gatggagctc gcgttgctcg cgctcgacta
     2821 tgcgggtcag ctcgcccgct atgacatcga atgctttctc gccggccccg gcgagacgcg
     2881 ccggcacatc aagccgccgg cgcgcgaccg tcgcttcgac ggcagcgagt ggctgcactg
     2941 gccgttcaac gtgctgcacc agtcgtttct cctcacggag caatggtggg cggcggccac
     3001 gcgcggcgtg ctcggggtcg agcgccatca cgaggagctg gtgtcgttcg ccgcccggca
     3061 atggctcgac ctcgcctcgc ccagcaacat gctcgccacc aacccggtgg cgctgaagaa
     3121 gacgctgcaa gagtgcgggc aaaatctggt gcgcggcgcg acgaacgcgc tcaccgacct
     3181 gcagcgcatg gcggcggggc tgccgccgcc gggcgcggag aagttcgtcg tcggcgcaaa
     3241 cgtcgccgtg acgccgggca aggtcgtgct caggaaccgg ctgatcgagc tgatccagta
     3301 ctcgcccgcc acgccccaag tatgccccga gccggtgctg ctcgtgcccg cctggatcat
     3361 gaagtactac atcctcgatc tctcgcccgc caactcgctc gtcaagtacc tcgtcgacca
     3421 gggccacacg gtgttctgca tctcgtggaa gaacccgaca gtggacgagc gcgacctcgg
     3481 aatggacgat tacctgcaac tcgggctcgg cgccgcgctc gacgccgtgc aggcgattgt
     3541 tccgcagcgc aaggtgcacg cgctcggcta ttgcctgggc ggaacgttgc tctcgatcgg
     3601 cgcggccgcc atggcgcgcg acaaggacga tcgcctggcc tcgctcacgc tgctcgccgc
     3661 gcagaccgaa ttcagcgagc ccggcgagct cggcctgttc atcgacgagt cgcaggtaag
     3721 cctgctcgaa gcgcagatgg cgcagaccgg ctatctcagc tcgcagcaga tgggcggcgc
     3781 gttccagctg ctgcgttcca acgacctgct ctggtcgcgg ctcgtcaacc agtggctgct
     3841 cggcgagccc agcgaggtga acgacctgat ggcgtggaac gcggatgcga cacggctgcc
     3901 ggcgcgcatg cattcggagt acctgcgcca gctcttcctg cacgacgacc ttgccgagag
     3961 ccgctaccgg gtcggcgggc ggctggtatc gctcgcggac atcaaggcgc ccatttttct
     4021 tgtcggcacg gtgaccgacc acgtcgcgcc ctggcgctcc gtctacaagc tgcaccacct
     4081 gacgccggcg gagatcacct tcgtgctcac gagcggcggc cacaatgccg ggatcgtgag
     4141 cccgccgggc catccgcggc gccactatca gctcctgacg agcccggcct ttaccggcca
     4201 gacagatggg cggaccgatc cggaccactg gctcgcccaa gcccccgagc accagggctc
     4261 gtggtggccc gcatggcacg agtggctgcg cgcgcactcg ggcgatccgg tcaagccgcc
     4321 gcgcacgggc ttgcccggag agcgtgtgct gggcgacgcg ccgggccgct acgtactgga
     4381 gaaatgaggc cgtcatgagc gaaaccccca tcctggctac cgcgcacaag gcggtcgatc
     4441 cgctgcccac cgtctgggat ccgaactcgc gcgtcggcga gatgctgaaa ggcaagcgcg
     4501 ggctcgtgat cggagtcgcg aatgccgaca gcatcgccta cggctgcgcg gtcaagctgc
     4561 gcgccttcgg cgcggacatc gcgctcacct acctcaacga caaggcgaag cagtacgtcg
     4621 cgccgctcgc cgagcagatc gacgcatcgc tcctcctgcc gctcgacgtc accaagcccg
     4681 ggcagatgca ggcggtgttc gagcgcatcc gcgccgactg gggacggctc gatttcgtca
     4741 tccactcgat cgccttcgcg ccgcgcgagg acctgcacgg ccgcgtggtg gactgctcgc
     4801 aggcgggctt cctgcaggca atgcaggtgt cctgccattc gttcctcgag atggcgcgct
     4861 gcgccgagcc gctgatgagc gagggcggca gcctcatcac gatgagctac tacggcgcgg
     4921 acaaggtggt gaacaactac aacgtcatgg gcccggtgaa ggcggcgctc gaggcctcgg
     4981 tgcggtatct cgcgaaagag ctcggcccgc gaaacattcg cgtgttcgcc gtgtcgcccg
     5041 ggccgctgcg cacgcgcgcc tcgagcggca tcgcgcactt cgacgagctg atcgacatgg
     5101 cggtgaagcg ctcgccgggc cagcgcctgg tcgacatcgc cgagatcggc cgggtggtcg
     5161 ccttcctcgc catgccggcg tcctcggcca tgaccggcga cgtggtctac atcgacgccg
     5221 gcctgcacaa catggcctga ccgagagtcg aacatgaaca gcacattcgc ttccgaggac
     5281 ctcgaggtcc tgcgcaaccg cactttcgac gagatcaagg ttggcgagcg cgcgagcatc
     5341 gagcgcacgc tgagcgcctc cgacatccag ctcttcgccg cgatgtcggg cgacatcaac
     5401 cctcagcaca tcgatgccga gttcgccgcg tcgacccgct tccagggcgt catcgcgcac
     5461 ggcatgtggg gcgccgcgct gatttccgcg gtgctcggca cacgcctgcc cggccccggg
     5521 acgatctatg ccggccagac gctgaagttc cacgcgccgg tgcgcgtcgg cgacacgctg
     5581 acgatcagcg tcgtcgtcac cgagcgtgac gaggccaagc atcggctggt cctcgactgc
     5641 aattgcacga accaggaagg caagacggtg atctccggcc aggcgacggt gatcgcgccc
     5701 accgagcgca tcgagcgccc gcgcggcacg ctgccgcagg tgcgcgtgtc gctgcagcac
     5761 ggcgacgagc tcgtgcgggt gctgcagtac gtgaagccgc tcgggcgcat ccgggtggcg
     5821 gtcgttcatc cctgcgacga ggtgagcctc tcggcggccc tcgacgcccg cagcgccggg
     5881 gtgatcgagc cggtcctgat cggcccgcgc gcgcgcctcg aggcggttgc gcagaaatgc
     5941 ggcgcgaacc tgcgggatgt ggcgatcgaa gacgtgccgc acagccacgc cgccgcgaca
     6001 cgcgcggtag agctcgcgct caagggcgag gtggaggcgc tgatgaaggg aagcctccac
     6061 accgacgagc tcatgagcgc cgtggtgaag agcgcgctgc gcaccgagcg gcgcatgagc
     6121 cactgcttca tcatgcagac gccggcctac ccgcgcccgt tcatcatcac cgacgcggcc
     6181 atcaacatcg cgccgacgtt gcaggacaag gccgacatca tccgcaacgc cgtcgacctc
     6241 gcgcacgcct tcggcatcgc gcggcctcgc gtcgccatcc tcgccgcggt ggagaccgtc
     6301 aacccgaaca tgccggccac gctcgacgcg gcggcgctct gcaagatggc cgaccgcggc
     6361 cagatcgccg gcgccatcct cgacgggccg ctcgccttcg acaacgcagt gtcggccgcc
     6421 gcggcgcgca ccaagggaat cgtctccgaa gtagccggcg cagccgacat cctcgtggtt
     6481 cccgacctcg aagcgggcaa catgctcgcg aagcagctcg agtacctcgg cggcgcggcg
     6541 agcgccggca tcgtgctcgg cgcgcgtgtg ccgatcgtcc tcaccagccg cgcggatgcg
     6601 cgcgaggcgc gcatcgcctc ctgcgcggtc gccgtggtgg tcgcgcggcg gatgggacag
     6661 ccggccgttg cgattgcagc gccgcgcaag caggccgcgg agcccgtcca atgaccccga
     6721 ccgggatctc cgatggctct cagtcctgaa tccaaatacc caagccggcg aacgtacgtc
     6781 gtgaaagtgc gaagcgatgc gaaggccggc gcgctcgccg g
//
LOCUS       DASUNT010000171         3101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_899221, whole genome shotgun sequence.
ACCESSION   DASUNT010000171 DASUNT010000000
VERSION     DASUNT010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899221"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VFX94_03330"
     CDS             <1..706
                     /locus_tag="VFX94_03330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5767243.1"
                     /translation="KVAVFVELHRKSERLRESEDKLRRLAAHLISVREDERAHIAREI
                     HDELGQVLTGHNMEVTWLAKRLKDKPLVEKTDTMCALIDSTVQTVRKIATGLRPEMLD
                     DMGLVAAVAWQAKEFQKRTGIRCRASLPPESKLDIDVSTTMFRIFQEILTNVARHSRA
                     TRVDIELEVGQSEVSLEVVDNGVGIQENELNARKSLGLLGMQERALLFGGDVAITGSA
                     GHGTRVSVTIPLRKPQ"
     gene            703..1332
                     /locus_tag="VFX94_03335"
     CDS             703..1332
                     /locus_tag="VFX94_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5767244.1"
                     /translation="MKVLIADDHPVVRQGLRQMLAVESDLTVVGEARTGQEVVELSRR
                     VPWEVAVLDYNMPGKNGLELIKELRQRYPGRAVLVLSMYPEDRYAVRALKAGAAGYLT
                     KESAPEELVSAIRKVAGGGRYVTPTLGEKLALELEDNRGKPLHETLSDREYQVMWMIA
                     SGKTVRQIADELFLSPNTVSTHRTRILRKMNMKSNAELMHYAIAHHLVD"
     gene            1415..>3101
                     /locus_tag="VFX94_03340"
     CDS             1415..>3101
                     /locus_tag="VFX94_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX5767245.1"
                     /translation="MDSMNPELQIMLDRGELLKALRAFKRGDFSVRMPLNLTGIDGEI
                     AQTFNDVVELEDAKAAEIARIADQVGREGQTAARMQLSSAMGGWAEVIHAMNRLINDM
                     TYPMSEVARVIGSVAKGDLSQTMVLQVDGRPLRGDALRLGTVVNTMVGQLNAFASEVS
                     RVAREVGSEGKLGGQAQVAGVAGVWKDLTDNVNSMAANLTGPVRNIAEVTTAVARGDL
                     SRKITGEVRGEILELKNTMNTMLDQLNAFASEVSRVAREVGTEGKLGGQAQVAGVGGV
                     WKDLTDNVNRLAANLTGQVRAIAEVATAVAKGDLTRSITVEALGEVAALKDNINEMIR
                     NLKEATRKSTEQDWLKTHLARFTRMLQGHRDLVTVSRLILSELTPLVNAQQAAFYVAD
                     HRNGGSHLELLAGYAQRQGRTLPRKIPFGEGLVGQCAFEKKRILLNSAPADYARIGSA
                     LGSGAPASIIILPVLFEGQVKAVIELASLSKFSEIHQSFLDQLTESIGVVLHTIAANM
                     QTEYLLHQSQSLTAELQSQQEELKKTNDRLEQQALNLQKSEGLLKSNQEELRSA"
BASE COUNT          596 a         1006 c         1047 g          452 t
ORIGIN      
        1 gaaggtcgcg gtgttcgtgg agctgcaccg caagagcgag cggctgcgcg agtccgagga
       61 caagctgcgc aggctcgcgg cgcacctgat ctcggtcagg gaggacgaaa gggcgcacat
      121 cgcgcgcgag atccacgacg agctcgggca ggtgctcacg ggccacaata tggaagtcac
      181 ctggctcgcc aagcggctga aggacaagcc cctcgtcgag aagaccgaca ccatgtgcgc
      241 gctcatcgac tcgacggtgc agaccgtgcg caagatcgcg accgggctgc gccccgagat
      301 gctcgacgac atggggctcg tcgcggcggt tgcctggcag gcgaaggagt tccagaagcg
      361 caccggcatc cgctgccgcg cgagcctgcc gcccgagagc aagctcgaca tcgacgtgtc
      421 gaccacgatg ttccgtatct tccaggagat cctcaccaac gtggcgcgcc attcgcgcgc
      481 cacgcgcgtg gacatcgagc tcgaggtcgg ccagtccgag gtctcgctcg aggtcgtgga
      541 taacggcgtc ggcatccagg aaaacgagtt gaacgccagg aaatcgctcg gcctgctcgg
      601 catgcaggag cgggcgcttc ttttcggcgg cgatgtggcg atcaccggct cggccggtca
      661 cggcacccgc gtctcggtca ccattcccct gaggaaaccg caatgaaagt gctcatcgcc
      721 gacgatcatc cggtcgtgcg gcagggcctg cgccagatgc tcgctgtcga gtcggacctg
      781 acggtggtcg gcgaggcgcg caccgggcag gaagtcgtgg agctctcgcg ccgcgtgccg
      841 tgggaagtgg cggtgctcga ctacaacatg ccgggcaaga acggcctcga gctcatcaag
      901 gagctgcgcc agcgctatcc gggcagggcg gtgctcgtgt tatccatgta tcccgaggat
      961 cgctatgcgg ttcgcgcgct gaaggccggc gccgccggct acctcaccaa ggaaagcgcg
     1021 cccgaggagc tggtgagcgc gatccgcaag gtcgccggcg gcggccgcta cgtcaccccc
     1081 acgctcggcg aaaagctcgc cctcgagctc gaggacaacc gcggcaagcc gctgcacgaa
     1141 acgctctccg accgcgagta ccaggtgatg tggatgatcg cttcgggcaa gacggtgcgc
     1201 cagatcgccg acgagctttt cctttcgccg aacaccgtca gcacgcatcg cacgcgcatc
     1261 ctgcgcaaga tgaacatgaa gtccaacgcg gagctgatgc actacgcgat tgcccaccac
     1321 ctcgtcgact aataaagggg gtcaggaccc tttttcaata aaagggtcct gacctttttc
     1381 tgtagtagta cgactgcaaa gccataacga cgccatggac tccatgaatc cggaactgca
     1441 gatcatgctg gatcgcggcg agctgctgaa ggcgctgcgc gcgttcaagc gaggcgactt
     1501 ttccgtgcgc atgccgctca acctgaccgg gatcgacggc gagatcgccc agaccttcaa
     1561 cgacgtggtg gagctcgagg acgccaaggc ggccgagatc gcgcgcatcg cggaccaggt
     1621 cgggcgcgaa ggccagacgg cggcgcgcat gcagctgagc agtgcgatgg gcggctgggc
     1681 cgaggtgatc cacgccatga acaggctcat caacgacatg acttacccga tgagcgaggt
     1741 ggcgcgcgtg atcggctcgg tggcgaaggg cgatctctcg cagacgatgg tgctccaggt
     1801 cgacggccgg ccgctgcgcg gcgatgcgct gcgtctcgga acggtcgtca acacgatggt
     1861 cgggcagctc aacgcattcg cctccgaagt ctcgcgtgtc gcacgcgagg tcggcagcga
     1921 aggcaagctc ggcggccagg cgcaggtggc gggcgtcgcg ggcgtgtgga aggacctcac
     1981 cgacaacgtg aactcgatgg cggcgaacct caccggcccg gtgcgcaaca tcgcggaggt
     2041 gaccaccgcc gtggcgcgcg gcgatctctc gcgcaagatc accggggagg tgaggggcga
     2101 aatcctcgag ctcaagaaca ccatgaacac gatgctggac cagctgaacg cgttcgcctc
     2161 cgaggtgtcc cgcgtcgcac gcgaggtcgg cacggaaggc aagctcggcg gccaggcgca
     2221 ggtggccggc gtcggcggcg tatggaagga cctcaccgac aacgtcaacc gcctcgcggc
     2281 caacctcact ggccaggtgc gcgcgatcgc cgaggtggcg accgcggtgg cgaagggcga
     2341 cctcacgcgc tccatcacgg tggaagcgct gggcgaggtg gccgcgctca aggacaacat
     2401 caacgagatg atccgcaacc tcaaggaggc gacgcgcaag agcaccgagc aggactggct
     2461 gaagacgcac cttgcccgct tcacgcgcat gctgcagggg catcgcgatc tcgtcaccgt
     2521 ttcacgcctg atcctgagcg agctcacgcc cctggtgaac gcccagcagg ccgccttcta
     2581 cgtcgccgac cacaggaacg ggggctcgca tctcgagctt ctcgccggct acgcgcagcg
     2641 ccagggcagg acgctgccgc gcaagatccc cttcggcgaa gggctggtcg gccagtgcgc
     2701 cttcgagaag aagcgcatcc tgctcaactc ggcgcccgcc gattacgcgc gcatcggctc
     2761 ggcgctcggc tcgggcgcgc ccgcgtcgat catcatcctg ccggtgctgt tcgaggggca
     2821 ggtgaaggcg gtgatcgagc tcgcgtcgtt gtcgaagttc agcgagatcc accagagctt
     2881 cctcgaccag ctcaccgagt cgatcggcgt cgtgctgcac acgatcgccg ccaacatgca
     2941 aaccgagtac ctgctgcacc agtcgcagtc gctcaccgcc gagctgcaga gccagcagga
     3001 ggagctgaag aagacgaacg accgcctgga gcagcaggcg ctcaacctgc agaagtccga
     3061 ggggctgctc aagtccaacc aggaggagct gcgctcggcg a
//
LOCUS       DASUNT010000172         3858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_548667, whole genome shotgun sequence.
ACCESSION   DASUNT010000172 DASUNT010000000
VERSION     DASUNT010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548667"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="VFX94_03345"
     CDS             <1..1066
                     /locus_tag="VFX94_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX5767246.1"
                     /translation="IAEMDNAVRVIDQQIERADAEVLRLNTELAADPIPSVREALGKA
                     VELRIGCEKALAGARNAVEAAAGALRELEAARLQAEASAAPLREKLGELRLKEQAARL
                     NYDQFATQLAEAGANDELLAAEAARAPRASSLQGEITRLAQATNELGAVNLAALEELS
                     ASEQRKSFLDAQATDLEEAVKTLEDAIRKIDRESRELLRETFDTVNAHLGRLFPTLFG
                     GGDAKLVMTGEEILDAGVQVMAHPPGKRNTSIHLLSGGEKALTAIALVFSLFQLNPAP
                     FCLLDEVDAPLDDSNTLRFCDLVKRMSAQTQFLFISHNKISMEMASQLVGVTMPESGV
                     SRVVAVDIEEALRIREELAA"
     gene            1073..1915
                     /locus_tag="VFX94_03350"
     CDS             1073..1915
                     /locus_tag="VFX94_03350"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HEX5767247.1"
                     /translation="MSDLVLGLLVLGVVLFAAVLLHNRLQERSARRAVERAFDSSRPG
                     DTLAPALPERREPTLERSSPRPLPDERVDYVVEIKPAASLAAPMMQEAWRSIDQRFGR
                     RALLAASAGGWRAALQLVSRAGAVTEAELVEFRTAVETLAAQLGATVAAPEMRPTLEA
                     ARELDRACADSDIQVALHVVGEGIEADLGEQPFQVVRREDGVTLMLDVALTPDLGASY
                     EAMASAGRRLAAAHNARLVDDRDNPLDERSLAAIGAQLEAVRQRLAARGIDTGSPLAL
                     RLFS"
     gene            1912..>3858
                     /gene="ligA"
                     /locus_tag="VFX94_03355"
     CDS             1912..>3858
                     /gene="ligA"
                     /locus_tag="VFX94_03355"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX5767248.1"
                     /translation="MNPAQRAKKLREAIERHNRLYYIEDAPEITDAEYDALFRELQSL
                     EAKHPELRSPDSPTQRVGAAPLAEFGEVRHRTPMLSISNASEEEEVHAFDRRVCQAVG
                     AERVEYAAEPKLDGLAVSLVYRDGAFVQGATRGDGTTGEDVTANLRTVRSIPLSIALK
                     GSLEARGEVLMYRRDFDALNKRQREAGEKEYVNPRNAAAGAVRQLDSRITASRPLRFF
                     AYAIVDAERVDWKTHAQALERLQSLGFPVCRERDVVEGPAGLLEYYRRIGRVRDTLPY
                     AIDGVVYKVNRLDWQERLGFVSRAPRFALAHKYPAEEQTTEVLGIEVQVGRTGALTPV
                     AKLKPVFVGGVTVTNATLHNEAELRRKDVRVGDTVIVRRAGDVIPEVVSVRKELRPPG
                     AREFQMPATCPVCNSAIVKNEDEAVHRCSGGLYCPAQRKQALLHFASRRAMNIEGLGE
                     RLVDQLVDRGLVANPADLYRLGPGELESLDRMGEKSAANLLRELERSRRTSLERLVYS
                     LGIRNVGESTARDLARHFGDLEPLARATEEELAQVPDVGPIVARSIRQFFDEAHNREV
                     IANLAARGVRWEKAAPAPRPAGPVKSFVLTGTLAGMTRDEARAAIEAKGHKLAGSVSK
                     KTDYVIAGEDAGSKLERARALGVPV"
BASE COUNT          624 a         1306 c         1391 g          537 t
ORIGIN      
        1 gatcgccgag atggacaacg cggtgcgcgt gatcgaccag cagatcgagc gcgccgacgc
       61 ggaggtgctc cgcctgaaca ccgagctcgc cgccgacccg atcccgtcgg tgcgcgaggc
      121 gctcggcaag gccgtggagc tcaggatcgg ctgcgaaaag gcgcttgccg gagcgcgcaa
      181 cgcggtcgaa gccgccgccg gcgcgctgcg cgagctggag gcggcgaggc tccaggccga
      241 ggcgagcgca gcgccgctgc gcgaaaagct cggcgagctg cgcttgaagg agcaggcggc
      301 gaggctgaat tatgaccagt tcgcgaccca gctggcggag gcgggcgcga acgacgagct
      361 cctcgccgcc gaggccgctc gcgcgccgcg cgcctcctcg ctgcagggag agatcacgcg
      421 gcttgcccag gcgacgaacg agctcggcgc ggtgaacctc gcggcgctgg aggagctcag
      481 tgcgagcgag cagcgcaaga gcttcctcga tgcgcaggcg accgacctcg aggaggcggt
      541 gaagacgctg gaggacgcga tccgcaagat cgaccgcgag agccgggagc tgctgcgcga
      601 aacgttcgac accgtgaacg cccacctcgg caggcttttt cccacgcttt tcggcggcgg
      661 cgacgcgaag ctcgtcatga ccggcgagga gatcctggac gccggcgtgc aggtgatggc
      721 gcacccaccg ggcaagcgca ataccagcat tcacctcctg tcgggcgggg agaaggcgct
      781 caccgcgatc gcgctcgtct tttcgctctt ccagctgaac ccggcgccct tctgcctgct
      841 cgacgaggtc gacgcgccgc tcgacgactc caacacgctg cgcttctgcg acctggtgaa
      901 gcgcatgtcg gcgcagaccc agtttctgtt catcagccac aacaagattt cgatggaaat
      961 ggcttcccag ctcgtcggcg tgaccatgcc cgagtcgggc gtctcgcgcg tggtggcggt
     1021 agacatagaa gaagcgctgc gcatacggga ggagcttgct gcgtagggcg tgatgagcga
     1081 cctcgtgctc ggtctgctcg tgctcggcgt ggtgctcttc gccgcggtgt tgctccacaa
     1141 ccgcctgcag gagcgctcgg cgcgccgcgc ggtggagcgc gccttcgact cctcgcgccc
     1201 gggagacacg ctcgctcccg ccctgccgga gcggcgcgag ccgaccctcg aaagaagctc
     1261 gccgcggccg ctgcccgacg agcgggtgga ctacgtggtc gagatcaagc cggcggcctc
     1321 gcttgccgcg cccatgatgc aggaagcgtg gcggagcatc gaccagcgct tcggccggcg
     1381 cgcactgctc gcggcttcgg ccgggggctg gcgcgctgcg ctccagctcg tgagccgggc
     1441 cggcgcggtg accgaagccg agctcgtcga gttccgcacg gcggtggaga cgctcgcggc
     1501 gcagctcggc gccacggtgg ccgcgccgga gatgcgtccg acgctcgagg ccgcgcgcga
     1561 gctcgatcgg gcatgcgccg acagcgatat ccaggtcgcg ctgcacgtgg tcggcgaagg
     1621 catcgaggcc gaccttggcg agcagccttt ccaggtcgtg cggcgcgagg atggggtaac
     1681 gctgatgctc gacgtggccc ttacgcccga ccttggcgcg agctacgagg cgatggcgag
     1741 cgccgggcgc aggctcgcgg ccgcgcacaa cgcgcggctc gtcgacgacc gcgacaaccc
     1801 gctcgacgag cgctcgctcg ccgcgatcgg cgcgcaactc gaggcggtgc gccagaggct
     1861 cgccgcgcgc ggcatcgaca ccgggagccc gctcgcgcta cggctcttct catgaatccg
     1921 gcgcagaggg cgaaaaagct gcgcgaggcg atcgagcggc acaaccggct ttactacatc
     1981 gaggacgcgc ccgagatcac cgatgccgaa tacgacgcgc tcttccgcga attgcagtcg
     2041 ctggaggcga agcaccccga gctgcgcagt cccgactcgc cgacgcagcg cgtgggcgcg
     2101 gctccgcttg ccgagttcgg cgaggtacgg caccgcacgc cgatgctttc catcagcaac
     2161 gcctccgagg aggaggaagt gcacgccttc gacaggcggg tttgccaggc ggtgggggcg
     2221 gagcgcgtcg agtacgccgc cgagcccaag ctggacgggc tcgccgtaag cctcgtctac
     2281 cgggatggcg ccttcgtcca gggggctacg cgcggcgacg gcactaccgg cgaggatgtc
     2341 accgcgaact tgcgcactgt tcgctcgata ccgctcagca tcgctctgaa gggatctctc
     2401 gaggcgagag gcgaagtgtt gatgtaccgc cgggacttcg atgcgctcaa caagcgccag
     2461 cgcgaagcgg gcgagaaaga gtacgtgaat ccgcgcaacg ccgcggccgg cgcggtgcgc
     2521 cagctcgact cgcgcattac cgcttcccgc ccgctgcggt ttttcgccta tgccattgtg
     2581 gacgcggagc gcgtggactg gaaaacgcac gcgcaggcgc tcgagcggct gcaatcgctc
     2641 ggattcccgg tatgcaggga acgcgacgtg gtggagggcc cggcgggcct tctcgagtac
     2701 taccgccgta tcggccgcgt gcgcgacacg ctgccgtacg cgatcgacgg cgtcgtctac
     2761 aaggtgaacc ggctcgactg gcaggagcgc ctgggcttcg tgtcgcgggc gccgcgattc
     2821 gcgctcgccc acaagtaccc ggcggaggag cagacgaccg aggtcctcgg catcgaggtc
     2881 caggtcggcc gcacgggagc gctcacgccc gtcgccaagc tcaagcccgt tttcgtcggc
     2941 ggcgtcacgg tgaccaacgc caccttgcac aacgaagccg agctgcgacg caaagacgtg
     3001 cgcgtcggcg atacggtgat cgtgcggcgc gcgggcgacg tgattccgga agtcgtgtcg
     3061 gtaagaaaag agctgcgtcc gcccggcgcg cgggagttcc agatgccggc cacctgcccg
     3121 gtgtgcaact ccgcgatcgt gaagaacgaa gatgaagccg tgcatcgctg cagcggaggt
     3181 ctttattgcc cggcgcagcg caagcaggcg ctgctccact tcgccagccg ccgcgccatg
     3241 aacatcgagg gcctgggcga gcgcctggta gaccagctcg tggaccgcgg cctcgtggcc
     3301 aacccggcgg acctgtatcg gctcggcccc ggcgagctcg aatcgctcga ccggatgggc
     3361 gagaaatcgg cggccaatct cctgcgcgag ctcgagcgct cgcggcgcac ctcgctggag
     3421 cggctggtct actcgctcgg catccgcaac gtgggcgaga gcacggcgcg cgacctcgcg
     3481 cgccatttcg gggacctcga gccgctcgcg cgcgcaaccg aggaggagct cgcgcaggtg
     3541 cccgatgtgg gcccgatcgt cgcgcgctcc atccggcagt tcttcgacga ggcgcacaac
     3601 cgcgaggtca tcgccaatct cgccgcccgg ggagtgcgct gggagaaggc ggcgcccgcg
     3661 ccgaggcccg cggggcctgt gaagagcttc gtgctaaccg gcacgctcgc cggcatgacg
     3721 cgcgacgagg cgcgcgccgc gatcgaggcg aaggggcaca agctcgccgg ctcggtgtcc
     3781 aagaagaccg attacgtcat cgccggcgag gatgccggct ccaagctcga gcgggcgcgt
     3841 gcgctggggg taccggtg
//
LOCUS       DASUNT010000173         4535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1075162, whole genome shotgun sequence.
ACCESSION   DASUNT010000173 DASUNT010000000
VERSION     DASUNT010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075162"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFX94_03360"
     CDS             <1..214
                     /locus_tag="VFX94_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5767249.1"
                     /translation="LAPLVREEIWRCLGRLREAGQTILVIDKYIERLVRLADRHTIIE
                     RGRVAWQGTSAELDADRGLWHRYLGI"
     gene            complement(438..1139)
                     /locus_tag="VFX94_03365"
     CDS             complement(438..1139)
                     /locus_tag="VFX94_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5767250.1"
                     /translation="MLEVENLETGYGTSQVLFGVGFSISPNEFHCLLGRNGMGKTTTV
                     RSILGLTRSFGGSITFRGEPIQRLAPDRIARMGIALVPEGRQIFPNLSVREHLVAFAE
                     NRSGRRDPWTPERVFQLFPRLRERQDNLGNQLSGGEQQMLAIGRALVTNPHLLILDEA
                     TEGLAPLVREEIWHCLGRLREAGQTILVIDKYIERLVKLAGRHTIIERGRVAWQGSSA
                     ELDADRALWHRYLGI"
     gene            complement(1132..1875)
                     /locus_tag="VFX94_03370"
     CDS             complement(1132..1875)
                     /locus_tag="VFX94_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5767251.1"
                     /translation="MLSLRSLSKRFGGVVAVDGVTLEVAAGEVHALIGPNGAGKTTLI
                     HQIAGSLPVDAGQIVFLGKDITRLPAHQRVAAGLARSYQITSIFRRFSVLDNLALAVQ
                     ARSGSSFSFWRPVAEEAALSEEAQAIASRIGLSERTGFLASSLSHGEQRALEVGLALA
                     TRPKLVLLDEPMAGMGPEESRRMIALISRIRAEVTVLLVEHDMDAVFRLADRISVMVN
                     GRLIATGSPEEIRLSGEVRKAYLGDELAA"
     gene            complement(1850..2785)
                     /locus_tag="VFX94_03375"
     CDS             complement(1850..2785)
                     /locus_tag="VFX94_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5767252.1"
                     /translation="MRRLIVIGALACAFPWVMQALDQHFYTSFATRILIYAMAAASLN
                     LVLGYGGMVSFGHAAFFGAGAYIVGILAAEGVTSLWLAWPLAIAVAALAAAAIGAVSL
                     RTRGVYFIMITLAFAQMMYYVFVSLKAYGGDDGLSLPGRSTGLGVSLSNDVLWYYLVL
                     AELAGVLYLLHRLVHSRFGRVIEAIRENETRATAVGYPVYRYKLVCFVISGAIAGLAG
                     ALIANQTSYVGPALLHWVQSGTLMIMVILGGVGRFWGGPVGAGVLLALEELFSGSPLL
                     GAYSLHWQLPVGVILLAVVLFAPQGIAGAFAKKPQ"
     gene            complement(2782..3702)
                     /locus_tag="VFX94_03380"
     CDS             complement(2782..3702)
                     /locus_tag="VFX94_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5767253.1"
                     /translation="MSAILLLEQTLNSLQFGLMLFLLAAGLTLVFGIMDMINLAHGSL
                     YMIGAYLMATFAEATGSFWMALPLALLATALVGAALEVSLLRKLYERDHLTQVLATFA
                     VILIANEVVRIIWGNQSLMLNPPSGLTGPVQLPGGVQYASYRLAIIAVGICVALLLYV
                     LVAKTRVGMLVRAGASNREMAVVMGVNIRRLFTVVFALGAALCALAGAMLGPLLAVQV
                     GMGENILILAFVVIVIGGIGSIRGAFVGALLVGLVDTMGRALLPIAFARMFSPQVASN
                     LGPSISSILIYVLMAAVLFLRPQGLFPARG"
     gene            complement(3761..>4535)
                     /locus_tag="VFX94_03385"
     CDS             complement(3761..>4535)
                     /locus_tag="VFX94_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767254.1"
                     /translation="KWNDRNKFYNDELSLTFLEEASLELVYWLEDAGYPALIVPPTHV
                     DPWRYDGDPDKHQRTLLSLPHAAVEAGLGTLGLNQQILTREFGPRVLLTAVLCSVDVE
                     CDKPLTEALCHGPECGRCLKTCPADAVREWDRDWEACDRYRSPHGFAQLCEYLARVID
                     EPDAARKKIMLRSEASFNLWQSILRGAGVITGCRRCADVCPVGADYAPMLKDAVEAIP
                     ENTPAKEARLAAMTKGDASPAYRREVHWVGRMNWRRNNP"
BASE COUNT          827 a         1515 c         1506 g          687 t
ORIGIN      
        1 ccttgcgccg ctcgtgcgcg aggagatctg gcgctgtctt gggcgtctgc gcgaagccgg
       61 tcagacgatt ctcgtgatcg acaagtacat cgagcgcctg gtgaggctcg ccgaccgcca
      121 taccatcatt gagcgaggac gcgtggcgtg gcagggaacc tcggccgagc ttgacgccga
      181 ccgcgggctc tggcatcgct acctcgggat ctagaatgga ttggccgaaa tcgcgggccg
      241 agatcgagcg cagctcttcc ttggtgagcg gcgggatgcg gtgccagacc tcctcgatcg
      301 gtcccctggg gatgcgcttc tttaggaaag gcgactgcag cgcgcgctcg accgccacgc
      361 gctttctctg gcgctggatg gtctcgacct gcgacaccga caccggccag cgcaggttca
      421 atcgctcgtg ccgagcttca tatcccgagg tagcgatgcc agagcgcgcg gtcggcgtca
      481 agctcggcgg aggagccttg ccacgccacg cgcccgcgct cgatgatggt gtggcggccg
      541 gcgagcttca ccaggcgctc gatgtacttg tcgatcacga ggatcgtctg cccggcctcg
      601 cgcaggcgcc cgaggcagtg ccagatctcc tcgcgcacga gcggcgcgag gccttccgtc
      661 gcttcgtcga gtatcagcaa atgcggattg gtcacgagcg cccgcccgat agcgagcatc
      721 tgttgctcgc cgccggaaag ctggttgccc aggttatcct gcctctccct caggcgcgga
      781 aagagctgga acaccctttc cggggtccag ggatcccgcc tgcccgagcg gttctccgcg
      841 aacgcgacga ggtgctcccg cacgctcagg ttggggaaga tctgtcgccc ctcgggcacg
      901 agcgcgatcc ccatgcgggc aatgcggtcg ggcgccaggc gctggatcgg ctcgccacga
      961 aacgtgatgc tgcccccgaa agaccgggtg aggccgagga tggagcgaac cgtggtcgtc
     1021 ttccccatgc cgttcctgcc aagcaggcag tgaaattcgt tgggcgaaat gctgaaaccg
     1081 acgccgaaca gaacctggct cgtcccatag ccggtctcca ggttttcgac ctcaagcagc
     1141 aagctcgtct cccaggtacg ctttgcgcac ttcgccgctc agtcttattt cttcgggcga
     1201 gccggtcgcg atcaggcgtc cgttcaccat gaccgagatg cggtcggcga ggcggaagac
     1261 ggcgtccatg tcgtgctcga cgaggagcac cgtcacctcg gcccggatgc ggctgatgag
     1321 cgcgatcatg cgcctggact cctccggccc catgccggcc atcggctcat cgaggagcac
     1381 gagtttcggg cgcgtggcga gggcgaggcc gacttcgagc gcgcgctgct cgccgtggga
     1441 aagcgacgag gcaagaaagc cggttctttc ggaaagccct atccgggaag caatcgcctg
     1501 cgcttcctcg gaaagcgccg cctcctcggc caccggccgc cagaacgaga agctcgagcc
     1561 gctgcgcgcc tgcacggcga gcgcgaggtt gtcgagcacg ctgaagcgcc ggaagatgct
     1621 cgtgatctgg tagctgcgcg cgagccccgc ggccacgcgc tggtgggcgg gaaggcgggt
     1681 gatgtccttc ccgagaaaaa cgatctgtcc ggcgtcgacg ggaaggctgc cggcgatctg
     1741 gtgaatgagg gtggtcttgc ccgccccgtt cgggccgatc agcgcgtgca cttcgccggc
     1801 cgccacctcc agcgtgacgc cgtcgaccgc caccacgccg ccgaagcgct tactgaggct
     1861 tcttagcgaa agcacctgcg attccctgcg gcgcgaagag caccacggcg agcagtatca
     1921 cgcccaccgg cagctgccag tgaagcgagt aggcgccaag gagcgggctg ccggaaaaaa
     1981 gctcctccag cgcgaggagc accccggcgc cgaccggccc gccccagaat cgcccgaccc
     2041 cgccgagaat taccatgatc atgagcgttc cggactgcac ccaatgaagc agcgcggggc
     2101 cgacgtagct cgtctgattg gcgatgagcg cgccggctag cccggcgatc gcgccggaga
     2161 tcacgaagca gacgagcttg tagcggtaca ccgggtagcc gaccgcggtg gcgcgcgtct
     2221 cgttctcgcg gatcgcctcg atcacgcggc cgaaacgtga atgcaccagg cggtggagca
     2281 gataaagaac tcccgcaagc tcggcgagaa ccaggtagta ccagaggacg tcgttcgaca
     2341 ggctcacgcc gagcccggtg gagcgcccgg gaagcgaaag cccgtcgtcg ccgccgtagg
     2401 ccttcagcga gacgaacacg tagtacatca tctgcgcaaa ggctagggtg atcatgatga
     2461 agtacacccc gcgcgtgcgc agcgagaccg ccccgatcgc agccgcggcg agcgccgcga
     2521 ccgcgatcgc gagcggccac gcgagccaga ggctcgtcac gccttccgcc gccaggatcc
     2581 cgacgatgta ggcgcccgcg ccgaagaacg ccgcgtggcc gaagctcacc atgccgccgt
     2641 agccgagcac gaggttgagg ctcgccgccg ccatggcgta gatcaggatg cgcgtggcga
     2701 agctcgtgta gaaatgctgg tcgagcgcct gcatgaccca gggaaaggcg catgcaagag
     2761 cgccgatgac gatgagcctt ctcatccgcg cgcggggaag aggccctgcg ggcgcaggaa
     2821 aagcaccgcc gccatgagaa cgtagatgag aatcgagctg atcgacggac cgaggttcga
     2881 ggcgacctgc ggcgagaaca tcctggcgaa agcgatcggc agcagcgccc gccccatggt
     2941 gtcgacgagc ccgacgagaa gcgcgcccac gaacgcgccg cggatcgagc cgatgcctcc
     3001 gatcacgatc accacgaagg cgaggatgag gatgttctcg cccatgccga cctgcactgc
     3061 gaggagcggc ccgagcatcg cgccggccag cgcgcagagc gccgcgccga gcgcgaagac
     3121 caccgtgaag agacgccgga tgttgacacc catcacgacc gccatctccc ggttcgaggc
     3181 gcccgcgcgc accagcatgc cgaccctggt cttcgccacc aggacataca gcaacaaggc
     3241 cacacaaatg cccacggcga tgatcgccag gcgataggac gcgtactgca cgccgcccgg
     3301 cagctgcacc gggccggtga gcccgctcgg cggattgagc atgagcgact ggttgcccca
     3361 gatgatgcgc accacctcgt tcgcgatcag gatgactgcg aacgtggcga gcacctgcgt
     3421 gaggtgatcg cgctcgtaaa gcttgcgcag gaggctcacc tccagcgcgg cgcccaccag
     3481 cgcggtcgcc aggagcgcga gcggcagcgc catccagaac gacccggtcg cctccgcgaa
     3541 cgtcgccatc aggtaggcgc cgatcatgta cagcgacccg tgcgccaggt tgatcatgtc
     3601 catgatcccg aacacgagcg tgagccccgc ggcgagcaga aacagcatca gcccgaactg
     3661 caggctgttc aacgtctgct cgagcaggag gatcgctgac atggaactcg gggacggagc
     3721 ctaattaatc aaggctccgt ccccgaattc cggtgcccct tcatggattg ttcctcctcc
     3781 aattcattct accaacccag tgcacctcgc gccggtacgc gggcgaagcg tcgcctttcg
     3841 tcatcgcggc gaggcgagct tccttcgcgg gcgtgttctc gggaatcgcc tcgacagcgt
     3901 ccttcagcat cggcgcgtaa tccgcgccca ccgggcagac gtcggcgcag cgccggcatc
     3961 cggtgatcac gccggcgccg cgcaggatgc tctgccagag gttgaagctc gcctcggagc
     4021 gcagcatgat tttcttcctg gcggcatcgg gctcgtcgat cacgcgcgcg agatactcgc
     4081 agagctgcgc gaacccgtgc ggcgagcgat agcggtcgca cgcctcccag tcgcggtccc
     4141 attcgcgcac ggcgtccgcg ggacaggtct tgaggcagcg tccgcactcg ggcccgtggc
     4201 agagcgcttc ggtcaggggt ttgtcgcact ccacgtcgac ggagcacaac accgccgtca
     4261 gcagaacgcg ggggccaaac tcgcgcgtga ggatttgctg gttcagaccc agcgtgccca
     4321 atcccgcctc cacggcggcg tgcgggagcg acaagagcgt gcgctggtgc ttgtctggat
     4381 cgccgtcgta gcgccacggg tcgacgtggg tcggcggcac gatgagcgcc gggtagcctg
     4441 cgtcctcgag ccagtacacg agctcaagcg aggcctcctc gaggaaagtg agcgaaagct
     4501 cgtcgttgta gaacttgttg cggtcgttcc acttg
//
LOCUS       DASUNT010000174         2939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_286460, whole genome shotgun sequence.
ACCESSION   DASUNT010000174 DASUNT010000000
VERSION     DASUNT010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286460"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /gene="hemF"
                     /locus_tag="VFX94_03390"
     CDS             complement(<1..679)
                     /gene="hemF"
                     /locus_tag="VFX94_03390"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEX5767255.1"
                     /translation="MDSREVHDYFAGLQERIVAALEVLDRKGFVRDSWQRAEGGGGTS
                     CILEDGQVFERGGVNFSRVHGARLPPSASAARPQLAGRAFEAMGVSLVLHPRNPYCPT
                     VHMNVRFFAAGDIWWFGGGMDLTPYYGFEDDARHFHATCKKALEPFGADYYPRFKRWC
                     DEYFYLRHRAEPRGIGGIFFDDLGEEGALELTRSVGDHFLAAYVPILERRKERAFGAR
                     EKAFQAYR"
     gene            complement(679..1953)
                     /gene="purD"
                     /locus_tag="VFX94_03395"
     CDS             complement(679..1953)
                     /gene="purD"
                     /locus_tag="VFX94_03395"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX5767256.1"
                     /translation="MKLLVVGSGGREHALSWKLAQSPRVQKVFVAPGNGGTATENGVE
                     NVALSGIEDLVSFAKKEKIHLTVVGPEAPLAAGIVDAFRDAGQKIFGPTRAAAQLEAS
                     KDFAKSFMARYRIPTAAHRTFDSVEAAKAYVAGRGAPIVVKADGLAAGKGVVVAASVP
                     EAQAAIERMMTDRSLGSAATRVVVEDFLEGEEASFIVMSDGTHVLALATSQDHKRLKD
                     GDAGPNTGGMGAYSPAPVVTPRIHARVMREIIVPAIQGMAQDATPYVGFLYAGLMIDK
                     AGNPRTLEFNCRLGDPETQPIILRLKSDLLELIERALEGTLDHVEPQWDRRAALGVVV
                     AAAGYPDEPRKGDPISGLPKPAPDCRVFHAGTRLEGTSLLTNGGRVLCVTALGDSVKR
                     ARARAYEALEGIRFEGMQYRKDIAYRALKKSP"
     gene            complement(1955..>2939)
                     /gene="purH"
                     /locus_tag="VFX94_03400"
     CDS             complement(1955..>2939)
                     /gene="purH"
                     /locus_tag="VFX94_03400"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX5767257.1"
                     /translation="IANYLSSRDGAFPETLTLQLQKLQELRYGENPHQRAAFYRDAHP
                     VGGSLASYRQLQGKELSYNNIADADAAWECVKSFAEPACVIVKHANPCGAASAGMLLD
                     AYEKALRCDPVSAFGGILAFNRALDRRTAEALGRQFAEVIIAPRVEPEAQDALAAKAN
                     LRVLEVPMSHEVQRYDYQRVGGGMLVQTSDAQLVEEKDLKVVTRKKPSDAQSSDLLFA
                     WRVAKFVKSNAIVFCAGGATLGIGAGQMSRLDSVRVAALKAKEAGLDLEGAVVASDAF
                     FPFRDGVDALADAGAAAVIQPGGSVRDAEVIAAADERGIAMLFTGMRHFRH"
BASE COUNT          438 a         1022 c          980 g          499 t
ORIGIN      
        1 ggcggtaggc ctggaacgcc ttttcgcggg cgccgaaggc gcgctccttg cgccgctcga
       61 ggatcggcac gtaggcggcg aggaaatggt cgccgaccga tcgggtgagc tctaaggcgc
      121 cttcttcccc gagatcgtcg aagaagatgc cgccgatccc gcgcggctcg gcgcggtgcc
      181 ggaggtagaa gtactcgtcg caccagcgct tgaagcgcgg atagtagtcg gcaccgaagg
      241 gctcgagcgc tttcttgcac gtggcgtgga aatggcgcgc gtcgtcctcg aagccgtagt
      301 acggcgtgag atccatgccg ccgccgaacc accagatgtc gcccgcggcg aaaaaccgca
      361 cgttcatgtg aaccgtcggg cagtacggat tgcgcgggtg caggacgagc gacaccccca
      421 tcgcctcgaa cgcgcgtccg gcgagctgcg gccgcgccgc gctcgccgaa ggcgggagcc
      481 ttgcgccgtg gacgcgcgag aagttcacgc cgccgcgctc gaacacctgc ccgtcttcca
      541 ggatgcagct tgtgccgccg ccgccttcgg cgcgctgcca ggagtcgcgc acgaagccct
      601 tgcggtccag cacttcgagg gcggccacga ttcgctcctg cagcccggcg aagtaatcgt
      661 ggacctcccg ggagtccatc acgggctttt cttcagcgcg cggtaagcga tgtccttgcg
      721 gtattgcatg ccctcgaagc gaatgccttc gagcgcttcg tatgctcgcg cacgcgcccg
      781 tttcaccgaa tcgccgagcg cggtaacgca cagcacgcgc ccgccattgg tgagcaggct
      841 cgtgccttcc agtcgcgtgc cggcatggaa cacgcggcaa tcgggggccg gcttgggcaa
      901 accgctgatc gggtctccct tgcgcggctc gtcggggtag cccgccgcgg cgacgaccac
      961 gccgagcgcc gcgcggcgat cccactgcgg ctcgacatgg tccagcgtgc cctccagcgc
     1021 ccgctcgatg agctccagga gatcggactt caggcgcagg atgatcggct gcgtctccgg
     1081 gtcgccgaga cggcagttga actcgagcgt cctcgggttt ccggccttgt cgatcatcag
     1141 gccggcgtag aggaagccca cgtacggcgt agcgtcctgc gccatgccct ggatggccgg
     1201 cacgatgatc tcccgcatga cgcgcgcgtg gatcctcggc gtgaccacgg gcgcagggga
     1261 gtacgcgccc atgccgcccg tgttcggccc ggcgtcgccg tccttgagcc gcttgtggtc
     1321 ctggctggta gcgagcgcga gcacgtgggt gccgtcgctc atcacgatga agctcgcttc
     1381 ttcgccttcg agaaaatctt cgaccaccac gcgtgtcgcg gcgctaccga ggctcctgtc
     1441 ggtcatcatc cgctcgatcg ccgcttgcgc ttccggcacg ctcgccgcca cgacgacgcc
     1501 cttgccggcg gcaaggccgt cggccttgac cacgatcggc gcgccgcgcc cggcgacgta
     1561 cgccttcgcc gcctcgaccg aatcgaacgt ccggtgcgcg gcggtgggga tccggtagcg
     1621 cgccatgaag ctcttggcga aatccttcga cgcttcgagc tgcgccgccg cgcgcgtcgg
     1681 gccgaagatc ttctggcccg cgtcgcggaa tgcatcgacg atgcctgcgg cgagcggcgc
     1741 ctccgggccg accaccgtca aatggatttt ttccttcttc gcaaaagaga cgagatcctc
     1801 gatcccgctc agtgcgacgt tctctacccc gttctccgtg gcggtgcctc cgttacccgg
     1861 ggcgacgaag actttctgca cccgcgggct ctgcgcgagt ttccacgaaa gggcgtgctc
     1921 gcgcccgccg gagccgacca cgagcaactt catctcagtg cctgaagtga cgcatgcccg
     1981 tgaaaagcat ggcgatgccg cgctcgtccg ccgcggcgat cacttcggcg tcacgcacgc
     2041 tgccgccggg ctggatcacc gccgccgcgc ccgcgtcggc gagggcgtcg acgccgtcgc
     2101 ggaatgggaa gaacgcatca gaggcgacca ccgcgccctc gaggtcgagc cccgcctcct
     2161 tcgccttcag cgcggcgacg cgcacgctgt cgagccggct catctgtccg gcgccgattc
     2221 ctagcgtggc gccgccggcg cagaacacaa tggcgttcga cttcacgaac ttcgccaccc
     2281 gccaggcaaa gagaagatcg ctgctctgcg cgtcgcttgg tttcttcctg gtgacgacct
     2341 tgaggtcctt ctcctcgact agctgcgcgt cgctcgtctg cacgagcatg cccccgccca
     2401 cgcgctggta atcgtagcgc tggacctcgt gcgacatcgg cacctcaagc acgcgcagat
     2461 tcgctttcgc ggcgagcgcg tcctgcgctt cgggctcgac ccggggcgcg atgatcacct
     2521 cggcgaactg ccggccgagc gcttcggcgg tgcgccggtc gagggcgcgg ttgaaggcga
     2581 ggatgccgcc gaaggccgac accgggtcgc agcgcaaggc cttttcgtat gcgtcgagga
     2641 gcatacctgc gctcgccgcg ccgcagggat tggcatgctt cacgatcacg caggcgggct
     2701 cggcgaagct tttcacgcat tcccaggccg cgtcggcgtc ggcgatgttg ttgtaggaga
     2761 gctccttgcc ctgcagctgg cggtagctcg ccaggctgcc gccgaccggg tgcgcgtcgc
     2821 gatagaacgc ggcgcgctgg tgcggattct ctccgtagcg cagctcctgc agcttctgca
     2881 gctgcagcgt gagcgtttcc ggaaaggcgc cgtcccggga ggaaaggtaa ttggcgatg
//
LOCUS       DASUNT010000175         4711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_812412, whole genome shotgun sequence.
ACCESSION   DASUNT010000175 DASUNT010000000
VERSION     DASUNT010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812412"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFX94_03405"
     CDS             <1..279
                     /locus_tag="VFX94_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX5767258.1"
                     /translation="EHQRAMETYWSLRWLLQERVTRLDAVLLRENLARVKGLPLVVRV
                     SAAPTLDPGTLVRMEITGIDLLERTLACAYRETLGRVQDATEEAGQKA"
     gene            317..1180
                     /locus_tag="VFX94_03410"
     CDS             317..1180
                     /locus_tag="VFX94_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX5767259.1"
                     /translation="MIWPKQDRVLGAALGLSLALHAGLLSLHFSFPEGVRWKQESAPL
                     EVVLVNTRTKERPAKADVLAQANLDRGGTVEERRRAKTPLPVTEPRTPGKDLADAQRR
                     QRSLEARQQELLAQARAAESAVPATPPREQGVEEAGAQPSGRDLADLTLAAMRLQAQI
                     DRQIEQYQKRPRKQFIGANAAEYRFAQYEEDWRLKIERVGTTNYPAEARGRLYGNLRL
                     TVTIRPDGSVESIELDRSSGLDVLDAAAFKIVRMATPFAAFPPDIRRDTDLLVITRTW
                     FFGQGDKIWTE"
     gene            1184..1978
                     /gene="aroE"
                     /locus_tag="VFX94_03415"
     CDS             1184..1978
                     /gene="aroE"
                     /locus_tag="VFX94_03415"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX5767260.1"
                     /translation="MSDRYAVIGNPVAHSRSPAIHAEFARATGEDIEYGRIESPLDGF
                     AREVESFRAAGGKGLNVTLPFKQEACRLCARLSGRARRAQAVNTLRFSDSFGDNTDGI
                     GLVRDISYNLQYALKDRSVAILGAGGAAQGVVGALMEAGAARLVVANRTLSKAQVLAS
                     RFPGISACGYEGLVGERFDIVINATSAGLSGAGPELPCGILGAWVLAYDMVYGRETRF
                     LAAARKAGARACDGLGMLVEQAAESFFVWRGVRPATGPVLAKLRGS"
     gene            1968..2672
                     /gene="mtgA"
                     /locus_tag="VFX94_03420"
     CDS             1968..2672
                     /gene="mtgA"
                     /locus_tag="VFX94_03420"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX5767261.1"
                     /translation="MARKRFRLLIAAWKAFCYTLGVLVVAAAAIQLWFLGHLVYWSQA
                     NPSSTAFMERYLEKPGARLRHTWVPYHRISDNLKRAVVAAEDARFLEHEGFDWEAIQE
                     AMRKNEKRGKVVTGASTISQQLAKNLFLSGSRSWLRKAQEAVITWMMERTLSKRRILE
                     IYLNVAEWGEGVFGAEAAARHHFGVPASALSIEQAAWLAAVLPSPRRYERGRETPYIA
                     GRVGTISARMAAAQIP"
     gene            2683..4050
                     /gene="mgtE"
                     /locus_tag="VFX94_03425"
     CDS             2683..4050
                     /gene="mgtE"
                     /locus_tag="VFX94_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX5767262.1"
                     /translation="MNEAPARLELEDLQHNLREMQALLQKHHVAELRGKLERLHPADI
                     AYILEALPLEERLALWNLVKADRDGEILVEVSDAVRETLIEAMDSRELVAAAETLETD
                     ELADIAPDLPQSVMAQVVQALPAEERERLRAALSFAEGTVGALMDFEHVSVREDVTLE
                     AVTRYLRRLDELPGHTDQLFVVDQDQVLKGTLPLARLIVSDLEREVRHVMVAERVKLY
                     AHDRAKDAASAFERYDLVSAPVVDATERLIGRLTVDAVVDYIRERSAESQLAEAGLSH
                     EEDVFAPVLDSFKNRWSWLAINLITAFIASRVIGAFENSIAELVALAALMPIVAGIGG
                     NSGNQTSTMIVRALALGQIEGTYWRRLLAKEIGVALLNGAVWGTLLGALAYAFYGNIA
                     LGGVLAAAMVLNLVLAALVGVAIPWLRARFGRDPAAGSSVLITGCTDSGGFFIFLGLA
                     TLFLV"
     gene            complement(4013..4564)
                     /locus_tag="VFX94_03430"
     CDS             complement(4013..4564)
                     /locus_tag="VFX94_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012362881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5767263.1"
                     /translation="MNIPIRELSRLEREALVPHFLALGADDRRLRFGAPLNDEAVRAY
                     VERIDFERDAVFGVYDDSLELIAAAHVARANGHAELGVSVLPGNRGRGIGGALLARAH
                     MRARNWGVNALFMHCLTENGAMMHLARKQRMRIVVESGEADAWLSLPPADASSHFGEA
                     FAQRVALFDYALKQGRAWLVPGI"
     gene            complement(4621..>4711)
                     /locus_tag="VFX94_03435"
     CDS             complement(4621..>4711)
                     /locus_tag="VFX94_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX5767264.1"
                     /translation="EAGFVSAAHDANVIDATVAAARAAFAKIS"
BASE COUNT          773 a         1529 c         1640 g          769 t
ORIGIN      
        1 gagcaccagc gcgccatgga gacctactgg agccttcgct ggctgctcca ggagcgagtc
       61 acgcggctgg acgcggtact actgcgcgag aatcttgctc gcgtcaaagg cctgccgctc
      121 gttgtgcgag tcagtgcggc accgacgctc gatccgggca ctctcgtgcg tatggaaatt
      181 acaggcatag acctgctcga gcgcacgctc gcctgcgcct accgcgagac tctcggccga
      241 gtgcaggatg caactgagga ggctggtcaa aaagcctaga atatgaaagg cttgttggac
      301 tgacgtgaga atgcacttga tatggccgaa gcaagaccgg gtgctcggcg cagcgctcgg
      361 actgtcgctc gccctgcacg cggggctgct ttcgctgcat tttagttttc cggaaggcgt
      421 gcgctggaag caggaaagcg cgccgctgga agtcgtcctc gtcaacacga ggaccaagga
      481 gcgcccggcc aaagccgacg tgcttgcgca ggcgaatctc gaccgcggcg ggacggtgga
      541 agaaagacgg cgggcgaaga cgccgttgcc ggtgaccgag ccgcgcactc cgggcaagga
      601 cctggcggac gcgcagcgcc gccagcgcag cctggaggcc cggcaacagg aattgctcgc
      661 gcaggcgcgt gcagcggaaa gcgccgtccc cgctacccca ccgcgcgaac agggcgtcga
      721 agaagccggc gcgcagccga gcggccgcga tctggccgac ctcacgctcg ccgcgatgcg
      781 gcttcaggcg cagatcgacc ggcagatcga gcagtaccag aagcgcccgc gcaagcagtt
      841 catcggcgcg aacgccgcgg agtatcgttt tgcgcagtac gaggaggatt ggcggctcaa
      901 gatcgagcgg gtaggaacga ccaactaccc ggccgaggcg cgcggcaggc tctacggcaa
      961 tctgcggctc acggttacga tccgccccga cggcagcgtc gagtcgatcg agctcgaccg
     1021 ttcttccggt ctggacgtgc tcgacgccgc ggcattcaag atcgtgcgca tggcgacgcc
     1081 gttcgccgcc tttccgcccg acatccggcg cgacaccgat ctcctggtca tcacgcgaac
     1141 atggtttttc ggtcagggcg acaagatctg gactgagtag agaatgagcg accggtacgc
     1201 ggtaatcggg aacccggtcg cgcattccag gtcgccggcg atccacgccg agtttgcgcg
     1261 cgccaccggc gaagacatcg aatatggccg tatcgagtcg ccgctcgacg gcttcgcccg
     1321 ggaggtagag agcttccgcg ccgctggtgg caaagggctc aacgtcacgc tgcccttcaa
     1381 gcaggaagcc tgtcggctgt gtgcccgact gagcgggcgg gcgcgccgcg cgcaggccgt
     1441 gaacacgctg cgcttttccg attcgttcgg cgacaacacc gacggcatcg gcctggtgcg
     1501 cgacatttcg tacaacctgc agtacgcctt gaaggaccga agcgtcgcca ttctgggcgc
     1561 cggcggggca gcgcaggggg tagtgggggc gctgatggag gccggtgcgg cgcggctggt
     1621 agtggcgaat cgcactttgt cgaaggctca ggtactggcc agccgctttc cgggcatttc
     1681 ggcttgcggg tacgagggcc tcgtgggcga gcgcttcgac atcgtaatca acgccacctc
     1741 ggcgggcctc tccggtgcgg ggccggagct gccttgcggg atccttggcg cgtgggtgct
     1801 cgcctacgat atggtgtacg ggcgcgagac gcgttttctc gccgccgcgc ggaaggcggg
     1861 cgcccgcgcc tgcgatgggc ttggcatgct ggtcgagcag gcggcggagt cgttcttcgt
     1921 ttggcgcggg gtgcgtccgg caacgggccc ggttctcgcg aagctgcgtg gctcgtaagc
     1981 ggtttcggct cctgatcgcg gcctggaagg cgttctgcta cacgctcggc gtgctcgttg
     2041 tcgcggccgc agcgatccag ctctggttcc tggggcacct cgtctactgg tcgcaggcca
     2101 atccgtcatc gaccgcgttc atggagcgct acctggagaa gcccggggcg cggctgcgcc
     2161 atacctgggt gccgtatcac cgcatctcgg acaatctgaa gcgcgcggtg gtggccgccg
     2221 aggacgcgag gttcctcgag cacgagggct tcgactggga agcgatccag gaagccatgc
     2281 gcaagaacga aaagcgcggc aaggtggtca ccggcgcgtc gacgataagc cagcagctcg
     2341 cgaagaatct ctttctctcc ggcagccgct cgtggctgcg caaggcgcag gaagccgtga
     2401 tcacctggat gatggagcga acgctctcca agcgccgcat cctcgagatc tatctgaacg
     2461 tggcggagtg gggcgagggc gtcttcggcg ccgaggcggc ggcgcgccac catttcggcg
     2521 tgccggcttc ggcgctaagc atcgagcagg cggcgtggct cgccgccgtc ctgccgagcc
     2581 cgcgtcgcta cgagcgcggc cgggaaaccc cgtacatcgc cggccgcgtg ggaaccattt
     2641 cggcgcgcat ggcggccgcc cagataccgt gagacgagcg ccgtgaacga ggcgcctgcg
     2701 cgcctggagc tcgaggacct gcagcacaac ctgcgtgaaa tgcaggccct gctgcagaag
     2761 catcacgtcg ccgagctgcg cggcaagctc gagcgcctgc accccgccga catcgcctac
     2821 attctcgagg cgctgccgct cgaggagcgg ctcgcgctct ggaacctcgt caaggccgat
     2881 cgcgacgggg agatcctggt cgaggtttcc gatgcggtcc gcgaaacgct catcgaggcg
     2941 atggattccc gcgagctcgt ggccgccgcc gagacgctcg agacggacga gctcgccgac
     3001 attgcgcccg acctgccgca aagcgtcatg gcgcaggtgg tgcaggcgct tcctgccgaa
     3061 gagcgcgagc ggctgcgcgc ggccctctcc ttcgcggagg gaacggtcgg cgcgctcatg
     3121 gacttcgagc acgtgtcggt gcgcgaggac gtgaccctgg aggcggtcac gcgctatttg
     3181 cgccggctgg acgagctgcc cggccacacc gaccagcttt tcgtggtcga ccaggaccag
     3241 gtgttgaagg ggacgctgcc gctcgcccgc ctgatcgtgt ccgatctcga gcgcgaggta
     3301 aggcacgtga tggtggccga gcgggtgaag ctctacgcgc acgatcgcgc caaggacgcg
     3361 gcgagcgcgt tcgagcgcta cgacctcgtg tccgcgccgg tcgtcgacgc gaccgagcgc
     3421 ctgatcggca ggctgaccgt ggacgcggtg gtcgactaca tccgggagag gagcgcggag
     3481 tcgcagctcg ccgaggcggg cctgagccac gaggaagacg tcttcgcacc ggtgctcgac
     3541 agcttcaaga accgctggtc ctggctcgcc atcaacctga ttaccgcgtt catcgcctcg
     3601 cgcgtcatcg gcgcgttcga aaattccatc gccgagcttg tcgcgctcgc cgcgctcatg
     3661 ccgatcgtcg ccgggatcgg cggcaactcg ggcaaccaga cgagcaccat gatcgtgcgc
     3721 gcgctcgctc tcgggcagat cgagggaacc tactggagac ggctgctcgc gaaggagatc
     3781 ggcgtcgcgc tgctcaacgg cgcggtgtgg ggcacgctgc tcggcgcgct cgcctacgcc
     3841 ttctacggca acatcgcgct gggcggggta ctcgccgcgg ccatggtgct caacctggtg
     3901 ctcgccgcgc tcgtcggcgt ggcgatcccg tggctgcgcg cgcgcttcgg gcgcgacccg
     3961 gccgcgggct cctcggtgct catcaccggt tgcacggact cgggcgggtt tttcatattc
     4021 ctgggactag ccacgctctt ccttgtttga gcgcgtagtc gaagagcgcg acccgctgcg
     4081 cgaacgcctc gccgaagtgg ctcgaagcgt cggcgggcgg gaggctcagc cacgcgtccg
     4141 cctcgcccga ctccacgacg atgcgcattc gctgcttgcg ggcaaggtgc atcatcgcgc
     4201 cattttcggt gaggcagtgc atgaagagcg cattgacgcc ccaattgcgg gcgcgcatgt
     4261 gcgcccgcgc taggagcgcg ccgccaatgc cgcggccgcg attgcccggc agcacggaaa
     4321 cgcccagctc cgcgtggccg ttggcgcgcg cgacgtgcgc cgctgcgatg agctcgagcg
     4381 aatcgtcata aacgccgaac actgcatcgc gctcgaaatc gatgcgctcg acgtaggcgc
     4441 gcaccgcctc gtcgttcaat ggcgcaccga atcgcagcct gcggtcgtct gcgcccaggg
     4501 cgaggaaatg cggcacgagc gcctcgcgct cgagcctgga gagctctctt attggtatgt
     4561 tcacggttgt tacctcgcgt attagacagc gcacagcacg caaggtttac accacttctg
     4621 ttaagagatt ttggcgaacg cggcgcgggc cgcggcgact gtggcatcga tcacgttggc
     4681 gtcgtgtgcc gccgatacga agcccgcttc g
//
LOCUS       DASUNT010000176         2054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1251351, whole genome shotgun sequence.
ACCESSION   DASUNT010000176 DASUNT010000000
VERSION     DASUNT010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251351"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1070)
                     /locus_tag="VFX94_03440"
     CDS             complement(36..1070)
                     /locus_tag="VFX94_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HEX5767265.1"
                     /translation="MAYRITLRPSGHTYECEEGKNVLQAGLEAGRMLPYSCRAGVCRT
                     CRGTILQGKVDYGRVHETYLPESDKAKGYALLCQATPRSDLVLEVREVQGVKPRLIPC
                     RVERLEKPAPDVALIGLRLPMNENFRFFAGQYIDILLKDGERRSYSLATRPAPGGVTA
                     LEIHVRHTPGGLFTDHVFGGLKVRDLLRFEGPLGSFYLREDSDKPVVMVASGTGFAPI
                     KAMCEYAFEKKLEQDRPITLYWGCRAKRDLYMLDLPRSWQRPGFEFVPVLSDPTPECR
                     WSECTGFVHRQVMADFPDMSGIQVYACGTPLMVEAAREDFSARCGLPPDEFYADSFLT
                     EADKAATATA"
     gene            complement(1084..1794)
                     /locus_tag="VFX94_03445"
     CDS             complement(1084..1794)
                     /locus_tag="VFX94_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5767266.1"
                     /translation="MLEARELRVTYGAAPAIDGVDLAVARGALVVVVGPNGAGKSTLI
                     NAIGGVHPYAGGDLRIDGKSLAGVPRHCFCDHGVALVPEGRRLFTTLTVRENLELGSY
                     RSDARKRRAQSLAEVCRLFPALEAKLDAPAGTLSGGQQQMVAIGRALMAQPKLLLLDE
                     PSLGLAPAIVLELFRIIREVNARGVAVLLVEQNVAAALDIAHRAYVLEEGRVVAEGEP
                     RALMSQTHIQKAYLGSEA"
     gene            complement(1787..>2054)
                     /locus_tag="VFX94_03450"
     CDS             complement(1787..>2054)
                     /locus_tag="VFX94_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5767267.1"
                     /translation="ASRPRLLFLDEVLSGLTPAEMDEAIRLVRAIRDQGATIVFVEHV
                     MRAVMELTDRVVVLTYGRAIAMGLPRDVMRDPEVVDAYLGTAHA"
BASE COUNT          349 a          670 c          693 g          342 t
ORIGIN      
        1 ctggatcttc tctcgggtga gcgacatgac tcctcctatg cggttgcggt tgcggccttg
       61 tcggcctcgg taagaaacga atcggcgtag aactcgtccg gcggcaggcc gcaccgggcg
      121 ctgaagtcct cgcgcgcggc ctcgaccatc aacggcgtgc cgcaggcgta cacctggatg
      181 cccgacatat cggggaaatc ggccatgacc tgccggtgaa caaagccggt gcactcgctc
      241 cagcggcatt cgggcgtggg atcggaaagc accggcacga actcgaaacc cggacgctgc
      301 cacgagcgcg gcaggtcgag catgtacagg tcgcgcttcg cgcggcatcc ccaatagagc
      361 gtgataggac gatcttgctc gagcttcttt tcgaacgcgt attcgcacat tgccttgatc
      421 ggcgcgaaac cggtgccgct cgcgaccatg acgacaggct tgtccgagtc ctcgcgcagg
      481 tagaagctgc cgagggggcc ttcgaagcgc agcaggtccc ggactttgag gccgccgaag
      541 acatgatcgg tgaagagtcc gccgggcgtg tggcgcacgt ggatctcgag cgctgtcacg
      601 ccgccaggcg ccggtcgcgt agcgagcgag taactgcggc gctcgccgtc cttgagcagg
      661 atgtcgatgt actgaccggc gaagaagcgg aagttctcgt tcatcggcag gcgcaggccg
      721 atgagcgcta cgtcgggcgc gggtttctcg agccgctcga cgcggcatgg gatgagccgt
      781 ggcttgacgc cctgcacctc gcgcacttcg agcacgaggt cgctgcgcgg cgtagcctgg
      841 caaagcagcg catagccctt cgccttgtcg ctctccggga gatacgtctc gtgcacccgc
      901 ccgtagtcca ccttgccttg gaggatggtg ccgcggcagg tgcggcagac gccggcgcgg
      961 cagctgtagg gcagcatgcg accggcttcg aggccggctt gcaggacgtt cttgccttcc
     1021 tcgcactcgt aggtgtggcc gctcggcctc agtgtgatgc gataagccat cagtgacctg
     1081 cctctaagcc tcgcttccta agtatgcctt ttgaatatgc gtttgcgaca tgagcgcgcg
     1141 cggctcaccc tcggctacga cccggccttc ctccagcacg taggcgcggt gcgcgatgtc
     1201 gagagcagcg gcgacgttct gctccaccag gagcaccgcg acaccgcgtg cgttgacttc
     1261 ccggatgata cggaaaagct cgagcacgat ggcgggggcg aggccgagcg aaggctcgtc
     1321 gagcaggagc agcttcggct gcgccatcag ggcccggccg atggccacca tctgctgctg
     1381 gccgcccgaa agcgtccccg cgggcgcgtc gagcttcgct tccagcgccg ggaacaatct
     1441 gcacacttcc gcgagcgact gcgcgcggcg tttgcgggca tcgctgcggt agctgccgag
     1501 ctccaggttc tcgcgcacgg tgagcgtcgt gaaaaggcgt cgcccctccg gcacgagagc
     1561 gacgccgtga tcgcagaagc aatggcgggg cacgccggcg agggatttgc cgtcgatgcg
     1621 caagtcgccg cccgcgtagg ggtgcacccc gccgatggca ttgatcagcg tcgacttgcc
     1681 cgcgccattc gggccgacga cgaccaccag cgcgcctctc gccactgcca ggtcgacacc
     1741 gtcgatcgcg ggcgcggcgc cataggtaac acgcagttcg cgcgcttcaa gcatgcgcgg
     1801 tccccagata ggcgtcgacc acttcagggt cacgcatgac gtctcgcggc agtcccatcg
     1861 cgatggcacg gccgtaggtc agcaccacga cgcggtcggt aagctccatg acggcgcgca
     1921 tgacgtgctc cacgaagacg atggtcgcac cctggtcgcg gatggcgcgc acgagcctga
     1981 ttgcctcgtc catttccgca ggcgtcaggc cggaaagcac ttcgtcgagg aagagcagcc
     2041 ggggacggga agcg
//
LOCUS       DASUNT010000177         3541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_374511, whole genome shotgun sequence.
ACCESSION   DASUNT010000177 DASUNT010000000
VERSION     DASUNT010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374511"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VFX94_03455"
     CDS             complement(<1..457)
                     /locus_tag="VFX94_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5767268.1"
                     /translation="MFSSPWWLPGGHLQTIYASLRPPPRVPLERSRWQTPDDDFVHVD
                     FAGERTAARRLVLFHGLEGCSDSHYARAIAAHAACRGWRIAIPHWRGCSGAPNRLPRA
                     YHSGDSAEVDWILRKLVASGRALHAAGVSLGGNALLKWLGEQGPAAVGVV"
     gene            complement(458..898)
                     /locus_tag="VFX94_03460"
     CDS             complement(458..898)
                     /locus_tag="VFX94_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5767269.1"
                     /translation="MSTRIFPTAQDAENAFYEALERCDLEGMMAVWAEDEDIVCVHPG
                     GGRLCGQDEVRESWARIFAAGPRARVAIEQQVALTGMMLAVHSVFERFVIPQAKPEQQ
                     PLPIIATNVYLRTAAGWRMIVHHASPAPAQPQSAPREAGPKILH"
     gene            complement(895..1833)
                     /gene="waaF"
                     /locus_tag="VFX94_03465"
     CDS             complement(895..1833)
                     /gene="waaF"
                     /locus_tag="VFX94_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEX5767270.1"
                     /translation="MPRVLVIAPNWIGDALMAQPLLSRLKQSGATIDVLAPEWVAPVL
                     HRMPEVDEVIAAPFRHGALQLGERWRLGRSLGRRGYEAAYVLPNSWKSALVPFFGRIP
                     RRIGYAGELRYGLLNRVFPKQESSMPRHYARLANGPDSALKPPQLIVWEEEIRETCRR
                     FGIGGHYTVLCPGAEYGPAKRWPYFKELAQRLGTQAVVLGSPKDAQAGAGLPGLNLIG
                     KTSLDEAIRIIAGASAVVSNDSGLMHVAAALGRPQVALFGSSSPEHTPPGSPAARVLW
                     LKLECSPCFERECPLGHFRCMKEITVEQVIAAMDRA"
     gene            complement(1836..2021)
                     /locus_tag="VFX94_03470"
     CDS             complement(1836..2021)
                     /locus_tag="VFX94_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEX5767271.1"
                     /translation="MADPRQVDVTEKDLPLHCPTPGTPLWALHPRVFLDVAREGETLC
                     PYCGTKYVYKGAPVKGH"
     gene            complement(2000..2923)
                     /locus_tag="VFX94_03475"
     CDS             complement(2000..2923)
                     /locus_tag="VFX94_03475"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX5767272.1"
                     /translation="MSMADRDGWIWHDGRLVEWRSATTHVLTHSLHYGLAVFEGVRAY
                     KTEIGTAIFRLKEHTERLFASAHIYMMKIPYTRERLMQAQAEVVRANGHEACYIRPIA
                     FYGSEKMGVSPVGAKVHVAIAAWPWGAYLGPEALSKGIRVKTSSYARHHINVTMARAK
                     MSGTYPNSVLATLEATMHGYDEGLLLDVDGFVAEGAGENVFIVKDGVLWEPELTSALT
                     GITRSSVIELAAELGCPVRAKRLTRDDIYIADEAFFTGTAAEVTPIRELDGRIIGEGK
                     AGPVTKAVQKAFFDVVTGKDRKRRKWLTPVK"
     gene            3029..3346
                     /locus_tag="VFX94_03480"
     CDS             3029..3346
                     /locus_tag="VFX94_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5767273.1"
                     /translation="MPVNSSAAHPQRVFYALASLPRRRILAFLSQTALSTAELAERFG
                     MSRPAVSRHLSVLQRAGLVGGERQGQRVLYRLRRDALLGALARFASEVDGPLPYRNPR
                     DAL"
     gene            3336..>3541
                     /locus_tag="VFX94_03485"
     CDS             3336..>3541
                     /locus_tag="VFX94_03485"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HEX5767274.1"
                     /translation="MRSSAALLALALLGACAGTRYATLVYVWLRSAPVATVLVSPYSE
                     RVRLLVVESGEQRVGQWINVRRNVV"
BASE COUNT          530 a         1211 c         1219 g          581 t
ORIGIN      
        1 gcaccacgcc cacggccgcc gggccctgct cgccgagcca cttcagcagc gcgttgccgc
       61 cgagcgagac ccccgccgca tgcagcgcac ggccggacgc tacgagcttt cgcaggatcc
      121 agtccacctc ggcgctgtcg cccgagtgat aggcgcgggg caggcgattg ggcgcgcccg
      181 agcagccgcg ccagtgcggg atcgcgatgc gccatccccg gcaggcggca tgggcggcga
      241 tggcgcgggc atagtggctg tcggagcagc cttcgagccc gtggaaaagc acgagccgcc
      301 gcgctgcggt gcgctcgccg gcgaagtcga cgtggacgaa gtcgtcgtcg ggtgtttgcc
      361 atcggctgcg ctcgagcggc acgcgcggcg gcgggcgcag cgacgcatag atcgtctgca
      421 ggtggccgcc gggcagccac cagggcgatg agaacatcta atgcaggatc ttggggccgg
      481 cctcgcgcgg ggcgctttgc ggctgcgccg gcgccggcga ggcgtgatgc acgatcatgc
      541 gccagccggc ggcggtgcgc aggtagacgt tcgtcgcaat gatcggcagg ggctgctgct
      601 cgggcttcgc ctgcgggatg acgaagcgct cgaacacgct gtggaccgcg agcatcatcc
      661 cggtgagagc gacctgctgc tcgatcgcca cgcgcgcgcg cggccccgcg gcgaaaatgc
      721 gcgcccagct ctcgcgcacc tcgtcctggc cgcacaggcg gccgccgccc gggtgcacgc
      781 agacgatgtc ctcgtcctcc gcccagacgg ccatcatgcc ctcgaggtcg cagcgctcga
      841 gcgcctcgta gaacgcgttc tccgcgtcct gcgcggtcgg gaagatcctt gtgctcaagc
      901 gcggtccatc gctgcgatga cctgctccac cgtgatctcc ttcatacaac gaaaatggcc
      961 gagcgggcat tctctttcga agcaagggct gcattccagc ttgagccaca gcacgcgcgc
     1021 cgccggcgag ccgggaggcg tatgttcggg gctcgaggaa ccgaagagcg cgacctgcgg
     1081 ccgcccgagc gcggcggcca cgtgcatgag gccggagtcg ttgctcacca ccgcggaggc
     1141 gcctgcgatg atgcgtatcg cttcgtcgag cgaggtcttc ccgatgagat tcagtcccgg
     1201 caggccggcg ccggcttgcg cgtccttcgg ggagccgagc accacggctt gcgtgccgag
     1261 tctttgcgcg agctccttga aatagggcca gcgctttgcc ggcccgtact cggcgcccgg
     1321 acagaggaca gtgtagtggc cgccgattcc gaagcggcgg cacgtctcgc ggatctcttc
     1381 ttcccagacg atgagctgcg gcggcttcag cgcgctgtcc ggcccgttcg cgaggcgcgc
     1441 gtaatggcgc ggcatcgaag attcctgctt gggaaaaacg cgattgagaa gcccgtagcg
     1501 cagctcgccc gcgtagccga tgcgtctggg aatgcggccg aaaaacggaa ccagcgccga
     1561 cttccagctg ttcggcagca cgtacgccgc ctcgtagccg cggcgcccga ggctgcggcc
     1621 gagccgccag cgctcgccga gctgcagcgc gccgtgcctg aagggcgcgg cgatgacctc
     1681 gtccacctcg ggcatgcgat gcaggacggg cgcgacccat tcgggcgcga gcacatcgat
     1741 agtcgcgccc gactgcttca accgcgaaag caacggctgc gccatcagcg cgtccccgat
     1801 ccagttcggc gcgatgacga ggacgcgcgg caaagctagt gtccctttac gggcgcgcct
     1861 ttatagacgt atttggtgcc gcaataggga cacagcgtct cgccttccct ggcgacgtcc
     1921 aggaacacgc gcgggtgcag cgcccacagc ggcgtgcccg gggtcgggca atgaaggggc
     1981 aggtccttct cggtgacgtc tacttgacgg gggtcagcca tttgcgtctt ttcctgtcct
     2041 tgccggttac gacgtcgaag aacgccttct ggactgcctt ggtgaccggg ccggccttgc
     2101 cctcgccgat gatgcggcca tcgagctctc ggatcggcgt gacctcggcc gcggtgcccg
     2161 tgaagaaagc ttcgtcggcg atgtagatat cgtcgcgcgt caggcgtttc gcgcgcaccg
     2221 ggcagccgag ctcggcggcg agctcgatca ccgaggagcg cgtgatgccg gtgagcgcgg
     2281 aggtaagctc cggctcccaa agcacgccat ccttcacgat gaagacgttc tcgccggcgc
     2341 cttcggccac gaagccgtcg acgtcgagca gcaggccctc gtcgtagccg tgcatcgtcg
     2401 cctccagtgt cgcgagcacc gagttcgggt aggtgccgga cattttcgcg cgcgccatgg
     2461 tgacgttgat gtggtggcgc gcataggacg aggtcttgac ccggatgccc ttggaaagcg
     2521 cttccgggcc gagataggcg ccccacggcc acgccgcgat ggcaacgtgg accttggcgc
     2581 ccacgggcga gacccccatc ttttccgagc catagaaagc gatcggccgg atgtagcacg
     2641 cctcgtggcc gttggcgcgc acgacctccg cctgcgcctg catcagccgt tcccgcgtgt
     2701 agggaatctt catcatgtag atatgcgccg aggcgaagag ccgctcggtg tgctccttca
     2761 gccggaagat cgcggtgccg atctcggtct tgtaggcgcg cacgccttcg aatacggcga
     2821 ggccgtagtg cagcgagtgc gtaagcacgt gcgtggtggc cgagcgccac tcgaccagtc
     2881 ttccgtcgtg ccagatccag ccatcgcggt cggccatcga cagcggcgcg gccgccgcct
     2941 tgatggcggc ccttcccttg gacatgatca tctctccgaa gtggagcgat tgtaatcaca
     3001 gcgccgcgga ctgcgccata ctattagcat gcctgttaat agttccgcgg cccatcccca
     3061 gcgcgtgttc tacgccctcg cctcgctgcc gcggcggcgc attctcgcgt ttctctcgca
     3121 gaccgcgctg tcgacggccg agctcgcgga gcgcttcggc atgagccggc cggcggtctc
     3181 gcgccacctg tcggtcctgc agcgcgcggg gctcgtcggc ggcgagcgcc aggggcagcg
     3241 cgtgctctac cggttgcggc gcgacgcgct ccttggcgcg ctcgcgcgct tcgcctccga
     3301 ggtggacggt ccgctgccgt accgcaaccc gcgcgatgcg ctctagcgcc gcgctcctcg
     3361 cgctcgcgtt gctcggcgcg tgcgcgggca cccggtacgc cacgctggtc tacgtctggc
     3421 tgcgcagcgc gcccgtcgcc accgtgctgg tcagcccata cagcgagcga gtgcgccttc
     3481 tcgtggtcga aagcggagag cagcgcgtcg gccaatggat caatgtgcgg cgcaacgtag
     3541 t
//
LOCUS       DASUNT010000178         2544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_374597, whole genome shotgun sequence.
ACCESSION   DASUNT010000178 DASUNT010000000
VERSION     DASUNT010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374597"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VFX94_03490"
     CDS             <1..638
                     /locus_tag="VFX94_03490"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5767275.1"
                     /translation="LVLERTLYRRLYRASDLDQCLFTIGIAFISIAAFAWFYTSEPQS
                     ARPPDWLRSTFSLGGVTFAGYRLFLILFGLAVTVALFFMLERTRFGARVRAAVDNQRM
                     ARGLGIDVDRAFMITFALGGGLAGLGGALAIEVIGLDPSFALSYLLVYVLIVVAVGGL
                     GSIGGSFAAALVLGITDTAGKYFFPWLGSFLIYLVMVALLMWRPHGLFGKR"
     gene            625..1587
                     /locus_tag="VFX94_03495"
     CDS             625..1587
                     /locus_tag="VFX94_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5767276.1"
                     /translation="MESASAHFQRKVRWHPLEIAFWLCTLLPFVVTPNYLSLASQIAI
                     TALFALSLDLILGYAGIVSLGHAAFFGVGAYTAGLLSKHYFGEPLTGLVVAAIAAGVV
                     GYATSHIIVRFRHLALIMLTMGLGLLLHEAANSASDLTGGADGLQGVKVGTLLGFRFD
                     MFGRTAYAYSLTVLFLCFLVCRRIIHSPFGLALRGIRENPTRMPAIGAPSRAHLRKAY
                     TISAVMAGIAGALLTHTTSTVSLETLSFGRSADVLVILILGGAGRLYGGIVGAIIYMV
                     ARDQFSGINPQYWYFWIGLLLVLVVMFLPNGILGGLSRFFQRKT"
     gene            1566..2306
                     /locus_tag="VFX94_03500"
     CDS             1566..2306
                     /locus_tag="VFX94_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5767277.1"
                     /translation="MLSTKNLNKNFGSLVVAQDIALELPPGVRYALIGPNGAGKTTLM
                     NLLTGMLRPDSGQVFLGGEEITSLRPDARVKKGLARTFQINTLFPQLSAIEAVALAVL
                     EREGAAHNWWRGLPRFTASIGEAHAILKSLGLESAAFRTTRELPYGQQRLLEIALALA
                     TRPRVLLLDEPAAGVPKEESAALFDAIANLSRQLTILFIEHDMDVVFRFASKIIVMVG
                     GRILVEGPPQDIARDERVREVYLGKAHA"
     gene            2299..>2544
                     /locus_tag="VFX94_03505"
     CDS             2299..>2544
                     /locus_tag="VFX94_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5767278.1"
                     /translation="MLELEAVRAGYGDAVVLDDISLEVPVGGALAVLGRNGVGKSTLL
                     LTIMGFTRMTRGSIHWRGEDITRLAPYKRARGGIGWVA"
BASE COUNT          353 a          891 c          801 g          499 t
ORIGIN      
        1 cgctcgtcct ggagcgcact ctttaccggc gcctgtaccg ggcttccgat ctcgaccagt
       61 gcctgttcac gatcggcatc gccttcattt cgatcgccgc gttcgcctgg ttctatacga
      121 gcgagccgca gtcggcgcgg ccgcccgatt ggctgcgcag cactttttct ctcggcggcg
      181 tcaccttcgc ggggtaccgg ctcttcctca tcctgttcgg cctcgcggtc accgtcgcgc
      241 tgttcttcat gctggagagg acgcgcttcg gcgcgcgcgt gcgcgccgcg gtggacaacc
      301 agcgcatggc gcgcggcctg ggcatcgacg tcgaccgcgc cttcatgatc accttcgcgc
      361 tcggcggcgg tctcgccggc ctcggcgggg cgctcgcgat cgaggtgatc gggctcgacc
      421 cgagcttcgc gctgagctat ctgctggtgt acgtcctcat cgtcgtggcg gtcggcggcc
      481 tgggttcgat cggcggctcg ttcgcggcgg ccctggtcct gggcattacc gatacggcgg
      541 ggaagtactt cttcccctgg ctgggctcgt tcctcatcta cctcgtcatg gtggcgctgc
      601 tcatgtggcg cccgcacggc ctgtttggaa agcgctagcg cccatttcca gcgcaaggtc
      661 cgctggcatc cgctggagat cgcgttctgg ctgtgcacgc tgctgccgtt cgtcgtcacg
      721 ccgaactacc tctcgctcgc gagccagatc gcgatcacgg cgctgttcgc gctgtcgctc
      781 gacctgatcc tcggctacgc cggtatcgtc tcgctcggcc acgctgcctt cttcggcgtc
      841 ggcgcttata ccgcgggcct gctctccaag cactacttcg gcgagccgct gaccggcctg
      901 gtcgtcgccg cgatcgccgc cggagtcgtc ggctacgcca cgagccacat catcgtgcgt
      961 ttccgccacc tcgccctcat catgctcacc atgggccttg ggctgctcct gcacgaggcc
     1021 gccaacagcg cgagcgatct caccggcggc gcggacggcc tgcagggcgt gaaggtcggc
     1081 acgcttctcg gctttcgctt cgacatgttc ggccgcaccg cctatgccta ctcgctcacc
     1141 gtcctgttct tgtgttttct ggtttgcaga agaatcatcc attcgccttt cggccttgcg
     1201 ctgcgcggaa tacgagagaa ccccacccgc atgccggcga tcggcgcgcc gagccgcgcg
     1261 cacctgcgta aggcctacac gatctcggcg gtcatggccg ggatcgcggg cgccctcctc
     1321 acccacacaa cctcgacggt gtcgctcgaa accctgtcct tcggccgctc ggccgacgta
     1381 ctcgtgatcc tcatccttgg gggcgccggc cggctctacg gcggcatcgt cggcgccatc
     1441 atctacatgg tcgcgcgcga tcagttctcc ggtatcaatc cccagtactg gtacttctgg
     1501 atcgggctgc tgctcgtgct ggtggtgatg tttctgccga acggcatcct cggaggcctt
     1561 tcaagattct ttcaacgaaa aacctgaaca agaacttcgg ctcgctcgtc gtcgcgcagg
     1621 acatcgcgct cgagctgccg ccgggcgtgc gctacgcgct catcgggccg aacggcgcgg
     1681 gcaagactac gctgatgaac ctgctcaccg ggatgctgcg ccccgattcg ggccaggtgt
     1741 tcctcggtgg ggaagaaatc acttcactgc gccccgatgc gcgggtgaag aaaggcctcg
     1801 cgcgcacgtt ccagatcaac accctcttcc cgcagctctc ggccatcgag gcagtggccc
     1861 tcgccgtgct cgagcgcgaa ggggcggcgc acaactggtg gcgcggcctg ccgcgcttca
     1921 cggccagcat tggggaagcc catgcgatct tgaagtcgct cggactggaa agcgcggcct
     1981 ttcggacgac ccgggaactg ccctacggcc agcaacgcct cctcgagatt gcgctcgcgc
     2041 tcgccacgcg tccccgcgtg ctgctgctcg acgagccagc cgcgggggtg ccgaaggagg
     2101 aaagcgcggc gctgttcgac gcgatcgcca acctttccag acagctgacc atcctcttca
     2161 tcgagcacga catggacgtc gtgttccgct tcgcctcgaa gatcattgtc atggtcggcg
     2221 gcaggatcct cgtcgaaggc ccgccacagg acattgcgcg cgacgagcgc gtgcgcgagg
     2281 tctatctggg gaaagcgcat gcttgagctc gaggcggtgc gcgcggggta cggcgacgcg
     2341 gtggtgctgg acgacatctc gctcgaagta cccgtgggcg gcgccctcgc cgttctcggg
     2401 agaaacggcg tcggcaagtc gacgcttctc ctcaccatca tgggcttcac ccgcatgacg
     2461 cgcggctcga tccactggcg cggcgaagac attacccggc tcgcgcctta caagcgcgcc
     2521 cgcggcggca tcggctgggt ggcg
//
LOCUS       DASUNT010000179         4503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1075603, whole genome shotgun sequence.
ACCESSION   DASUNT010000179 DASUNT010000000
VERSION     DASUNT010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075603"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VFX94_03510"
     CDS             complement(<1..567)
                     /locus_tag="VFX94_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5767279.1"
                     /translation="MRTLLLLLVCAGHALAQPYPSKPIRLVVGFPPGGAADFVSRAFQ
                     EPLGRALGQPIVVENRPGAGSSIAAEHVAKSAPDGYSVLIASPSSILVNPIITPKAGF
                     QPLKDLIPVSKVSSSPLILAVNPAVPAASVKELIAHAKQNPGKLNFATSGNGSAPHLA
                     AVLFVRLAGVDMVHVPYKGGAPAVQSVLA"
     gene            complement(564..845)
                     /locus_tag="VFX94_03515"
     CDS             complement(564..845)
                     /locus_tag="VFX94_03515"
                     /inference="COORDINATES: protein motif:HMM:NF040120.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HEX5767280.1"
                     /translation="MAVPPVDAAVRSPHRARALHEASQPERAREFRRAELKRLMVQPY
                     GRVVVLHLAIILGGMAAMALGSPMCSLIVLLALKVALDFRAHVKEHSSS"
     gene            complement(873..2150)
                     /locus_tag="VFX94_03520"
     CDS             complement(873..2150)
                     /locus_tag="VFX94_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5767281.1"
                     /translation="MELSLQVGKYDVQKLLGKGASGTVYLAKDTFTGKEVALKTIEPE
                     VFRDPQFGTVYRAQFVNEASLAGKLRHPHIIGILDAVVGEDSGHIAMELVTGGDLSQH
                     CSPETLLPVADVLQIGFKCCGALDYAAKEGIVHRDIKPANIMLAGGTDPKIADFGAAF
                     LKKTQAVQTAALGSPYYMAPEQIDGSKPLTFHTDMYSLGVVLYELLTGKRPFVGDNIQ
                     DLMNRILKLEPLPPSEARSGLPKSVDAVVLRALRKDPGSRYASWADFALELSKLGELV
                     LQPGAIPDSEKYVSLKKVSMLSTLADAELWELARAGRWRRVPKGSAVVQEDESGRSFF
                     FLAKGEAKVTKNRKLLNMVSSGECFGEMAYIGTGAEQRRHATVEAMSELLLAEFEPAA
                     LDTMSLNAQLQLTRALMRNVTDRLALANVRLVR"
     gene            complement(2150..3586)
                     /locus_tag="VFX94_03525"
     CDS             complement(2150..3586)
                     /locus_tag="VFX94_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5767282.1"
                     /translation="MGIHQNFINGEWALTREQSPNINPSNLADVVGDFARADAAQARA
                     AIAAARAAFPAWSRGPVQARADMLDRIGNEILARKDELGTLLAREEGKTRPEAVGEAT
                     RAGYLFKFFAGEVVRQAGEVLPSVRPGVKVEITREPVGVVGIITPWNFPLAIPAWKIA
                     PALAYGNCVVFKPADLVPGCAWALTEIIAKAGVPAGVFNLVMGRGSVLGELLSTDPGI
                     DAVSFTGSQAVGKRVAQNCAARFAKVQLEMGGKNPQVVLDDADLDTAVSVSVQSAFFS
                     TGQRCTASSRLIVTDGIHDRFVKALTEKMQKLKVDDALKEGTDIGPVVDERQLEQDLK
                     YIELGRKEGGRLAAGGSRLEREKQGYYLEPALFVDTTPQMTINREEIFGPVAAVIRVR
                     NYDEALAVANDTPFGLSSGIATTSLKHAEHFKRNSAAGMVMVNLPTAGVDYHVPFGGR
                     KGSSYGPREQGRYAVEFYTTVKTSYINP"
     gene            3596..>4503
                     /locus_tag="VFX94_03530"
     CDS             3596..>4503
                     /locus_tag="VFX94_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767283.1"
                     /translation="MIFDPAQIQRGARLRRSPYFEATQRAGCRGYTVYNHMLLPIRYD
                     SFEREYERLLADVTLWDVSVERNVEIAGRDGARFADLLTPRELSRCAVGEGRYVLLTD
                     EAGGIVNDPVLLRLEENRFWLACADSDVLLWAKGVARGSGMQVELRELDVAPLQVQGP
                     KSRQLVTELFGEAVSSLKYYEFRKAELRGIPVIVTRTGWTGELGYELYLLDPARGVEL
                     WDHVMAAGKAYNIAPTGPSDIRRIEAGILNYGIDMTLDNNPYAVGLERLVNLDKKADF
                     IGREALRRIAQDGVKRKLVGVEIGGTP"
BASE COUNT          736 a         1489 c         1517 g          761 t
ORIGIN      
        1 ggcgagcacc gactgcactg cgggcgcacc gcccttgtaa ggcacatgca ccatgtcgac
       61 gccggcgagc cgcacgaaga gcacggcggc gaggtgaggc gccgagccgt tgcccgacgt
      121 ggcgaaattg agcttgcctg ggttctgctt cgcatgcgca atcagctcct tcaccgatgc
      181 tgccggcacg gccgggttga ccgcgaggat gaggggcgat gagctcacct tgctgacggg
      241 aatgaggtcc ttcagcggct ggaagcccgc cttcggggta atgatcgggt tcacgagaat
      301 gctcgaaggg ctcgcaatca gcacgctgta gccgtcgggc gcgctcttcg cgacgtgctc
      361 ggccgcgatg ctcgaacccg cgcccgggcg attctccacc acgatcggct gcccgagcgc
      421 ccttccgagc ggctcctgga acgcgcgcga cacgaaatcc gcggcgccgc caggcggaaa
      481 gccgacgacc agacggatag gcttcgacgg ataaggctgg gcgagcgcgt ggcctgcgca
      541 cacgagcaac agaaggagag ttctcatgag ctcgagtgtt ctttgacgtg cgccctgaag
      601 tcaagcgcga ctttgagcgc gagaagcacg atgagcgaac acatcggcga tccaagcgcc
      661 atggccgcca tgccgccgag gatgatcgcg aggtgcagta ccacgacgcg gccgtacggc
      721 tgcaccatca ggcgcttgag ctcggcgcgg cggaattcgc gcgcgcgctc aggctgggat
      781 gcttcgtgga gagctcgcgc gcgatgtggc gagcgaacag cggcgtcaac aggcggaaca
      841 gccagggcgg cccctggatg gcgctcgcgc tcctagcgga cgagccggac gttggcgagc
      901 gcaagccggt cggtgacgtt tcgcatcagc gcgcgcgtga gctgaagctg cgcgttcagg
      961 ctcatcgtgt cgagcgccgc cggctcgaac tcggcgagca gcagctcgct catcgcctcg
     1021 accgtcgcgt ggcggcgctg ctcggcgccg gtgccgatgt acgccatctc gccgaagcac
     1081 tcgccgctgc tcaccatgtt gaggagcttg cggttcttgg tgaccttggc ctcgcccttg
     1141 gcgaggaaga aaaagctcct gccggactcg tcttcctgca cgaccgcgct gcccttcggc
     1201 acgcggcgcc agcgtccggc gcgcgcgagc tcccagagct cggcgtcggc gagcgtggaa
     1261 agcatggaaa ctttcttcag cgacacgtat ttttcgctgt ccgggatcgc gcccggctgc
     1321 agcacgagtt cgccgagctt cgagagctcg agcgcgaaat ccgcccagct cgcgtagcgc
     1381 gaccccgggt ctttcctcag ggcgcgcagc accacggcat cgaccgactt cggcagcccc
     1441 gagcgcgcct cgctcggcgg cagcggctcg agcttgagga tgcggttcat caggtcctgg
     1501 atgttgtcgc cgacgaacgg gcgcttgccg gtgagcagct cgtagagcac tacgccgagg
     1561 gaatacatgt cggtgtgaaa ggtgagcggc ttgctgccgt cgatctgctc gggcgccatg
     1621 taatacggcg agccgagcgc ggcggtctgc accgcctggg tcttcttcag gaaggcggcg
     1681 ccgaaatcgg cgatcttcgg gtcggtgccg ccggccagca tgatgttcgc cggcttgatg
     1741 tcgcggtgga cgatgccctc cttcgccgca tagtcgagcg cgccgcagca cttgaagccg
     1801 atctgcagca cgtcggccac cgggagcagc gtctcgggcg agcagtgctg cgagaggtcg
     1861 cccccggtga cgagttccat cgcgatatgc ccggagtcct cgcccaccac cgcatcgaga
     1921 atgccgatga tgtggggatg gcgcagcttg ccggcgagcg aggcttcgtt gacgaactgc
     1981 gcgcggtaga cggtaccgaa ctgcggatcg cgaaacacct cgggctcgat cgtcttgagc
     2041 gcgacctcct tgccggtaaa cgtgtccttc gcgaggtaga ccgtgcccga cgcgcccttg
     2101 ccgaggagct tctgcacgtc gtatttcccg acctggaggg acaactccat tacgggttga
     2161 tgtagctcgt cttcaccgtg gtgtagaact cgactgcgta gcgcccctgc tcgcgcggcc
     2221 cgtagctcga gcctttgcgg ccgccgaacg gcacgtggta gtccaccccc gcggtcggca
     2281 ggttgaccat caccatgccg gcagcggagt tgcgcttgaa gtgctcggcg tgcttgagtg
     2341 aagtggtcgc aatcccggag gacaggccga acggcgtgtc gttcgccact gcgagcgcct
     2401 cgtcgtagtt cctgacccgg atcacggccg cgaccggccc gaagatctcc tcgcggttga
     2461 tcgtcatctg cggcgtggta tcgacgaaga gcgccggctc gaggtaatag ccctgctttt
     2521 cgcgctcgag gcggctgcct ccggcggcga gcctgccgcc ctccttgcgt ccaagctcga
     2581 tgtacttcag gtcctgctcg agctggcgct cgtcgacgac cgggccgatg tcggtccctt
     2641 ctttcaatgc gtcgtcgact ttcagctttt gcattttctc cgtcaacgct ttcacaaatc
     2701 gatcatgaat cccgtcggtc acgatcaggc ggctggaggc cgtgcagcgc tgcccggtgg
     2761 agaagaacgc gctctggacg gacacactca ccgcggtgtc gaggtcggcg tcgtcgagca
     2821 ccacctgcgg gttcttgccg cccatctcga gctgcacctt ggcaaaccgc gccgcgcagt
     2881 tctgcgcaac acgcttgccc acggcctgcg atccggtgaa gctcacggcg tcgatgcccg
     2941 gatcggtgga gagcagttct ccgagcaccg agccgcggcc catcacgagg ttgaatacgc
     3001 cggccggtac gccggccttg gcgatgattt ccgtgagcgc ccaggcgcag cccggtacga
     3061 ggtcggcggg cttgaacacc acgcagttgc cataggcgag cgcgggggcg atcttccacg
     3121 ccgggatcgc gagcgggaag ttccacggcg tgatgatgcc caccacgccg accggctcgc
     3181 gggtgatctc gaccttgacc cccggccgca ccgacggcag cacctcgccc gcctggcgca
     3241 ccacctcgcc tgcgaagaac ttgaaaagat agcctgcgcg cgtcgcctcg ccgaccgctt
     3301 cgggcctggt cttgccctcc tcgcgggcaa gcagcgtgcc gagctcgtcc ttgcgggcga
     3361 ggatctcgtt gccgatgcgg tccagcatgt cggcgcgcgc ctgcaccggg ccgcgcgacc
     3421 atgccgggaa ggcggcgcgc gccgcggcga tggccgcgcg cgcctgcgcg gcgtcggcgc
     3481 gcgcgaagtc gccgacgacg tcggccaggt tcgacgggtt aatatttggc gactgctcgc
     3541 gcgttagcgc ccattcccca ttgataaaat tttgatgaat tcccatggca ctgtaatgat
     3601 attcgatccc gcccaaatcc agcgtggagc ccgtctcaga cgctcgccct atttcgaggc
     3661 gacgcagcgc gccggctgcc gcggctatac cgtgtacaac cacatgctcc tgccgatccg
     3721 gtacgactcc ttcgagcgcg agtacgagcg gctgctcgcc gacgtgacgc tgtgggacgt
     3781 ttcggtcgag cgcaacgtcg agatcgccgg ccgcgacggc gcgaggtttg ccgacctcct
     3841 caccccgcgc gagctctcga ggtgcgcagt cggggaaggg cgctacgtgc tcctcaccga
     3901 cgaggccggt ggcatcgtca acgacccggt gctgctgcgc ctggaggaga accgcttctg
     3961 gcttgcgtgc gccgacagcg atgtgctgct gtgggcaaaa ggcgtcgcac gcggcagcgg
     4021 catgcaggtc gagctgcgcg agctcgacgt cgcgccgctc caggtgcagg gcccgaagtc
     4081 gcgccagctg gtgacggagc tcttcggcga agcggtttcc agcctgaagt actacgagtt
     4141 cagaaaagcc gagctgcggg gcattccggt gatcgtcacg cgcaccggat ggacgggcga
     4201 gctcggctac gagctctacc tcctcgaccc cgcgcgcggg gtcgagctct gggaccacgt
     4261 catggcggcg ggcaaggcgt ataacatcgc gcccaccggc ccgtccgaca tccgccgcat
     4321 cgaagccggc atcctgaact acgggatcga catgacgctc gacaacaatc cgtacgcagt
     4381 gggactcgag cgcctcgtca acctcgacaa gaaggccgat ttcatcggcc gggaggcgct
     4441 gcgcaggatc gcgcaggacg gggtgaagcg aaagctggtc ggggtcgaga tcggcggaac
     4501 gcc
//
LOCUS       DASUNT010000180         2687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_462238, whole genome shotgun sequence.
ACCESSION   DASUNT010000180 DASUNT010000000
VERSION     DASUNT010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462238"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="VFX94_03535"
     CDS             complement(<1..1185)
                     /locus_tag="VFX94_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5767284.1"
                     /translation="MTHVFHRDPRLTYRVAVRGEGAYLIDRDGKRYLDASGGAAVSCL
                     GHSDAAVIQAIQRQLERLPFAHTSFFTNEPMEELADALIARAPKSFDKVYFVSGGSEA
                     IEAALKLARQYFVEKGEPQRAHVIGRRQSYHGNTLGALAVGGNAWRRKQFEPLLVRTT
                     HVSPCYAYRGKNPGESDAAYVERLGAELEAQFQQKSVIAFVAETVAGATIGAVPPVPG
                     YFRRVRELCDRYGVLLILDEVMCGMGRCGTLWTFEQEGIVPDLVAIAKGLGAGYQPIG
                     ALVVSKEIADTIQRGSGFFQHGHTYIGHAAACAGALAVQKRLHDDGVLARVTPLGRAL
                     ETRLRDAFAEHPHVGDIRGRGLFWGVEIVEDRATKRPFDPKLRMHARLKRKALEAGLM
                     CYP"
     gene            1262..2221
                     /locus_tag="VFX94_03540"
     CDS             1262..2221
                     /locus_tag="VFX94_03540"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5767285.1"
                     /translation="MFRILAVAAAAALLSGAATAQNKIKYALGDVIGIDDLPLLVAAE
                     RSKKRGVEVEITPFKNEDIATQAVINGQADIGQGTPYAAIEKVNVPIRFFVQLWSLQF
                     FPVVANEHYKSWKDLDGQEIAVQGRGSGTEAIMLLMAKQHGIKYKNVSYVPGSQVRAL
                     ALIKGNIRASILDATNKNFLMREAPGKFTILPLGEIKASDESLFARLEYLQKNQAAVQ
                     VFVEELVRVNREINKNPMSIVEERKKLGLLKDLPAKLEEELPTYFKEGVAGGIFPNDG
                     GAENAAKTDLEFFALSGSIKKENPKVDDYWHLAPLKAALSRLK"
     gene            2226..>2687
                     /locus_tag="VFX94_03545"
     CDS             2226..>2687
                     /locus_tag="VFX94_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5767286.1"
                     /translation="MRGWQLASFALFFGLWELAGRWPVSAAFPPFSAAFLALVEMLRD
                     GSLLKAYLITLQPLALGIVLCAVAGIGFGIGMGLSRSVEWLSLPIFIILQAAPMAAII
                     PLITYIYGIGLTSKVLAVIMLAAPVIVMNSYKGIRNTPPSLLQMCRSFQGTR"
BASE COUNT          475 a          881 c          873 g          458 t
ORIGIN      
        1 cgggtagcac atgaggccgg cctccaacgc cttgcgcttc aagcgcgcgt gcatgcgcag
       61 cttgggatcg aagggccttt tcgtcgcacg gtcttcgacg atctccacgc cccagaaaag
      121 gccccgcccg cgaatgtcgc cgacgtgcgg gtgctcggcg aaagcatccc gcaaccgcgt
      181 ttccaatgcg cgtcccagcg gcgtgacccg ggcgagcaca ccgtcgtcgt ggaggcgctt
      241 ttgcaccgcg agcgctccgg cgcaggccgc ggcgtggccg atgtacgtat gcccgtgctg
      301 gaagaaaccg ctgccgcgct ggatcgtatc ggcgatctcc ttcgacacca cgagcgcgcc
      361 gatcggctgg tagcccgcgc cgagcccttt cgcgatcgcg acgaggtccg gaacgatgcc
      421 ttcctgctcg aaggtccaga gcgtgccgca gcgccccatg ccgcacatca cctcgtccag
      481 gatgagcagc acgccgtagc ggtcgcaaag ctcgcgcacg cgcctgaagt agcccggcac
      541 cggcggtacc gcgccgatcg tcgcgcccgc caccgtttcg gcgacgaagg cgatcacgct
      601 cttctgctga aactgcgctt cgagctccgc gccgaggcgc tcgacatacg ctgcgtcgct
      661 ctccccgggg ttcttgccgc gataggcata gcagggcgat acgtgcgtcg tcctcaccag
      721 caaaggctcg aactgcttgc gccgccaggc gttgccgccg accgcgagcg cgccgagggt
      781 gttgccgtga taggactggc gccggccgat gacgtgcgcg cgctgcggct cgcctttctc
      841 gacgaagtac tgccgcgcga gcttgagcgc ggcctcgatc gcttccgacc cgccggagac
      901 gaagtacacc ttgtcgaagg acttcggcgc gcgcgcgatg agcgcgtcgg cgagctcttc
      961 catcggctcg ttcgtgaaga acgaggtgtg cgcgaagggc agcctctcca gctggcgctg
     1021 gatcgcctgg atcaccgcgg catcggagtg cccgaggcag gagaccgcgg cgccgcccga
     1081 agcatcgaga tagcgcttac cgtcccggtc gatgagataa gcaccctcgc cgcgcaccgc
     1141 cactcgatac gtcaggcgcg gatcgcggtg gaagacgtgc gtcacggcgt caaacatagc
     1201 atgtatggca gtaagattgc ctccgcacga gtttctaacg tccatttccc caggaggagt
     1261 gatgttcaga atccttgcag tcgcggcggc cgcagcgctg ttgagcggcg cagcaacggc
     1321 acagaacaag atcaagtacg cgctcggcga cgtcatcggg atcgacgacc tgccgctgct
     1381 cgtcgccgcg gagcgctcga aaaagcgcgg cgtcgaggtc gagatcacgc cgttcaagaa
     1441 cgaggacatt gccacgcagg cggtgatcaa cggccaggcc gacatcggcc agggcacgcc
     1501 gtacgcggcg atcgagaagg tgaatgtgcc gatccgcttc ttcgtgcagc tttggtcgct
     1561 gcagtttttc ccggtggtgg cgaacgagca ctacaagagc tggaaggacc tcgacggcca
     1621 ggagatcgcc gtgcagggcc gcggctcggg caccgaggcg atcatgctgc tcatggcgaa
     1681 gcagcatggc atcaagtaca agaacgtgag ctacgttccc ggctcgcagg tgcgtgcgct
     1741 cgcgctgatc aaaggcaaca tccgcgcctc gatcctcgac gcgacgaaca agaacttcct
     1801 gatgcgcgaa gcgcccggca agttcaccat cctgccgctc ggtgagatca aggcgagcga
     1861 cgagtcgctg tttgcgcggc tggagtacct gcagaagaac caggctgcgg tgcaggtgtt
     1921 cgtcgaggag ctggtgcgcg tcaatcgcga gatcaacaag aacccgatgt cgatcgtcga
     1981 agagcgcaag aaactcggct tgttgaaaga cctccccgcg aagctcgagg aggagctgcc
     2041 aacgtacttc aaggaaggcg tcgccggcgg catcttcccg aacgacggcg gcgccgagaa
     2101 cgcggccaag accgacctgg agttcttcgc gctctcgggc tcgatcaaga aagagaaccc
     2161 gaaggtcgac gactactggc acctcgctcc gctcaaggca gctctgtcca ggctgaagta
     2221 gcgctttgcg gggctggcag ctagcgtcgt tcgcgctctt cttcggattg tgggagctgg
     2281 ccggccgctg gccggtcagc gccgcctttc cgccgttcag cgcggctttc ctcgcgctcg
     2341 tggagatgct gcgcgacggc agcctgctca aggcctacct gatcacgctg cagccgctcg
     2401 cgctcggcat cgtgctctgc gccgtggcgg gcatcggctt cggcatcggc atggggcttt
     2461 cgcgctcggt ggagtggctg tcgctgccga tcttcatcat cctgcaggcc gcgccgatgg
     2521 cggcgatcat cccgctcatc acctacatct acggcatcgg cctaacttcg aaggtgcttg
     2581 cggtgatcat gctcgcggcg ccggtgatcg tgatgaattc gtacaagggc atccgcaaca
     2641 cgccgccttc gctactgcag atgtgccgct ctttccaagg cacgcgg
//
LOCUS       DASUNT010000181         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_900262, whole genome shotgun sequence.
ACCESSION   DASUNT010000181 DASUNT010000000
VERSION     DASUNT010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900262"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1176)
                     /locus_tag="VFX94_03550"
     CDS             complement(10..1176)
                     /locus_tag="VFX94_03550"
                     /inference="COORDINATES: protein motif:HMM:NF021427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEX5767287.1"
                     /translation="MARTIIVEAEEKVACPKCSHRFPLSEGISRQAIERHAADLERAL
                     AEAKKKLETQLAAEAKAQFDTQVKALNEALSAKEGTLAKFRTEELLLRKQLREVEEAR
                     KNQDLEYQRKLDAERKKIEEQARSAAGEEVSRREAQWKAQLESAQREAADLKRKLEQG
                     SQQLQGEALELSLEALLKSAFPLDEILPVPKGVNGADLVQRVRSPSGMVCGTILWEAK
                     QTKAWQPAWVRKLKDEQQEIGAEFGVIVTAAMPKDAREPFLREADVWVARFDAARPLA
                     ELLRSTLVELHKQRQANAGRSEKMELLYAYICSPQFASRLKTVYEGFVTMREELEAEK
                     AALARIWKKREAQLTRMQDGLLSVVGDLQGIGEDALPALDVLAALPAAEKIVEI"
     gene            1432..1566
                     /locus_tag="VFX94_03555"
     CDS             1432..1566
                     /locus_tag="VFX94_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767288.1"
                     /translation="MEPTNNEFNEDADLELDELETWDADDLREELEELQPGFDFGKSG
                     "
     gene            1660..>2542
                     /locus_tag="VFX94_03560"
     CDS             1660..>2542
                     /locus_tag="VFX94_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767289.1"
                     /translation="MPPTSTLVTDRDDTLGRDPYVQGTPGGPTAVSWGAVFAGAAAAA
                     ALSFVLLLLGTGLGLSSLSPWADEGASGKALGIGTFVWVIFMQLAASALGGYIAGRLR
                     TKWVSLHTEEVRFRDTAHGFLAWGVATLFTAWLVASTTTSLVSSGVQAGAQVAGGAAS
                     AATAAGAAKAGSEASTSNGDSGPMGYMVDSLFRGSKAKPPASGEQSTGEVTRIFTNAM
                     VTGKLPEQDARYAAQVVAQRTGVSQQEAEKRVNDTFSQVQKKKEETEVKAREAADAAR
                     KASATAALALFISLLIGA"
BASE COUNT          386 a          838 c          875 g          443 t
ORIGIN      
        1 taaagccgac taaatctcga cgattttctc ggccgcaggt agggccgcga ggacgtcaag
       61 cgcggggagc gcgtcctcgc cgatgccttg gagatcgccc acgacgctca gaaggccgtc
      121 ctgcatgcgg gtgagctgcg cttcgcgctt cttccagatg cgggcgagcg cggccttctc
      181 ggcttccagt tcctcgcgca tcgtgacgaa gccctcgtag acggtcttca ggcggctcgc
      241 gaattgcggg ctgcagatgt aggcgtagag gagctccatc ttttcgctgc ggccggcgtt
      301 cgcctggcgc tgcttgtgga gctcgacgag cgtcgagcgc aggagctcgg cgagcgggcg
      361 ggcggcgtcg aagcgggcga cccagacgtc cgcttcgcgc aggaagggtt cgcgcgcgtc
      421 tttgggcatc gccgcggtga cgatgacgcc gaactcggcg ccgatctcct gctgctcgtc
      481 cttgagcttg cgcacccagg ccggctgcca ggccttggtc tgcttcgctt cccagaggat
      541 cgttccgcac accattccgg agggcgagcg cacgcgctgc acgaggtcgg cgccgttcac
      601 gcccttgggg accggcagga tctcgtcgag cgggaacgcg ctcttgagga gcgcctcgag
      661 cgagagctcg agcgcttcgc cctggagctg ctgcgagccc tgctcgagct tgcgcttcaa
      721 gtcggccgcc tcgcgctgcg cggactcgag ctgcgccttc cactgggctt cgcgccggct
      781 tacttcctcg ccggcggccg agcgcgcctg ctcttcgatt ttcttgcgct cggcgtcgag
      841 cttgcgctgg tattcgaggt cctggttctt gcgcgcctct tccacttcac gaagctgctt
      901 gcgcagcaag agctcctcgg tgcggaactt ggcgagcgtg ccctccttcg cggagagtgc
      961 ctcgttgagc gccttcacct gcgtgtcgaa ctgggccttg gcttcagcgg cgagctgggt
     1021 ttcgagcttc ttcttcgctt cggcgagcgc gcgctcgaga tcggcggcgt ggcgctcgat
     1081 ggcctggcgg gagatgcctt cggacagcgg gaaccgatgg ctgcacttcg ggcaggcgac
     1141 cttctcctcc gcctccacga tgatggtccg ggccatacac gcctcctcga gcgggtatta
     1201 aggaggcctc atgctgcgcc gggtagtggc tcagggcgtg agcctgcgcg cgactacccg
     1261 tccacgatcc agaggcagaa gaggacgagg gtggggacga gggctaccaa caagtcgagc
     1321 gggatcatgg gaggcaggct agcggggcgc tggctcaggg gatgagccca tggtccgatc
     1381 gaaccaaaac cggaggctgc gttatgcgac atccggcgat tagatagcgc catggaaccc
     1441 accaacaacg aattcaacga agacgcggac ctcgagctcg acgagctcga gacctgggac
     1501 gccgacgatc tgcgcgagga actcgaagag ctgcagcccg ggttcgactt cggcaaatcc
     1561 ggctagcctc gtagcgggca cgcagcagaa atagcaggcc gccgcgcgcg gtcgggcacg
     1621 gccattgcga gattaggggt ctttttcagg aagatcccta tgccgcccac ctctaccctc
     1681 gttactgacc gcgacgacac gctcggccgc gacccctatg ttcaaggcac gcccggagga
     1741 ccgaccgcgg tctcctgggg tgcggttttc gccggcgctg ccgccgcggc cgcgctgtcc
     1801 ttcgtcctgc ttctcctcgg cacgggcctc ggcctgtctt ccctctcgcc ctgggcggac
     1861 gagggcgcga gcgggaaggc gctgggcatc ggcaccttcg tctgggtgat cttcatgcag
     1921 ctcgcggctt ccgccctcgg cggctatatc gcgggccggc tgcgcaccaa atgggtctcg
     1981 ctgcacacag aggaagtcag gttccgcgat accgcgcacg gttttctggc gtggggcgtg
     2041 gcgacgctct tcactgcctg gctcgtcgcg tccacgacga cctcgctggt gagtagcggc
     2101 gtgcaggccg gcgcgcaggt cgccggtggc gccgcgagcg cggcgacggc ggccggcgcg
     2161 gccaaggccg gctcggaggc atccacatcg aacggcgata gcggcccgat gggctacatg
     2221 gtcgattccc tcttccgcgg ctccaaggcc aagccgcccg cttcgggcga gcagtcgacc
     2281 ggcgaggtga cgcgcatctt caccaacgcg atggttaccg ggaagctgcc cgaacaggac
     2341 gcgcgctatg ccgcgcaggt cgtcgcgcag cgcaccggcg tgtcgcagca ggaagccgag
     2401 aagcgcgtga acgacacctt cagccaggtc cagaagaaga aggaggagac tgaggtcaag
     2461 gcgagagagg ccgccgacgc ggcgcgcaag gcatctgcca ccgcggcgct cgcgctcttc
     2521 atctcgctcc tgatcggtgc ct
//
LOCUS       DASUNT010000182         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_987698, whole genome shotgun sequence.
ACCESSION   DASUNT010000182 DASUNT010000000
VERSION     DASUNT010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987698"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2098)
                     /gene="ileS"
                     /locus_tag="VFX94_03565"
     CDS             complement(<1..2098)
                     /gene="ileS"
                     /locus_tag="VFX94_03565"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX5767290.1"
                     /translation="MADYKDTLNLPETPFPMRGDLARREPQWVKQWQEKGVYRRLRGL
                     ANGRPRFVLHDGPPYANGDIHIGTAVNKILKDIVVKSKTLAGFDAPYVPGWDCHGMPI
                     EVQIEKKYGKNLPTEETLRLCRAFATEQIERQKKDFGRLGVLGDWDQPYTTMAPRTEA
                     QEIRVLGRLLEKGYVSRGLKPVNWCFDCGSALAEAEVEYEDRRDPAVDVAFPVSGRDV
                     QQLGAAFNVPINKTACAVIWTTTPWTLPANQALNVHPDFSYDLVDTDRGLLILATDLK
                     DACLKRYGLSGRTVAQCKGRALENVRFRHPFYDREAPVYLGEYVTLETGTGIVHSAPA
                     YGVEDFESCRRYGMRDADILAPVMGDGRFAPSLALFGGLSIWDANSKIVKAIEERGAL
                     LAVDWKHTHSYMHCWRHKTPVILRATTQWFAAMDKPVDGESLRTTALRGIDNTQFFPS
                     WGKARLHGMIANRPDWTLSRQRQWGVPMPFFLHRETGALHPRTPELLEQVAKRVEKDG
                     IEAWHTMDKEALLGTDANDYDKSRDTLDVWFDSGSTHFTVMRGSHAADTRFPAELYLE
                     GSDQHRGWFHSSLLVSCMLDGVPPYKGLLTHGFVVDGEGRKMSKSKGNVVAPQEVSNR
                     LGAEILRLWVASTDYSGELSISNEILNRVVESYRRVRNTLRFLLANTSDFDLRRDAVA
                     GNDLVEIDRYALAMTVA"
     gene            complement(2091..>2498)
                     /locus_tag="VFX94_03570"
     CDS             complement(2091..>2498)
                     /locus_tag="VFX94_03570"
                     /inference="COORDINATES: protein motif:HMM:NF013821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HEX5767291.1"
                     /translation="LAAGDLARAERLLGRRYSMSGRVAHGERLGRELGFPTANIVLRR
                     APPLAGIFVVEVDKYGRGVASIGRRPTINPVAIPLLEVHLFDHDSDLYGEHLRVRFLK
                     KLRDEQKYDGLPALQAAIARDARQAREFFLSHG"
BASE COUNT          411 a          830 c          822 g          435 t
ORIGIN      
        1 tcgccaccgt catggcaagc gcgtagcgat cgatctctac cagatcgttg ccggctactg
       61 catcgcgtcg gaggtcgaag tccgaggtgt tggcgagaag gaagcgtagc gtgttgcgca
      121 cacgccggta gctctcgacc acgcgattca ggatctcgtt ggagatggac agctctccgg
      181 agtagtccgt gctcgccacc cacaggcgca ggatctcggc gccgaggcgg ttcgacacct
      241 cctgcggcgc gaccacgttg cccttcgact tggacatctt gcgcccctcg ccgtcgacca
      301 cgaagccgtg cgtgagcaac cccttgtacg gcggcacgcc gtcgagcatg caggagacga
      361 gaagcgagga atggaaccag ccgcggtgct ggtccgaccc ctcgagatag agctccgcgg
      421 ggaaacgggt gtccgcggcg tgcgaaccgc gcatcaccgt gaaatgcgtc gagccggaat
      481 cgaaccagac atcgagcgtg tcgcgactct tgtcgtagtc attggcatcc gtaccaagca
      541 gggcttcctt gtccatggtg tgccaggcct cgatgccgtc cttctcgacg cgcttcgcaa
      601 cctgctccag cagctcgggc gtgcgcggat gcagcgcgcc ggtctcgcga tgcaggaaaa
      661 acggcatcgg caccccccac tgccgctggc gcgacagcgt ccagtcgggc cggttggcga
      721 tcatgccgtg caagcgcgcc ttgccccagg aggggaagaa ctgggtgttg tcgatgccgc
      781 gcagagcggt ggtgcgcaag gactccccgt cgaccggctt gtccatcgcg gcgaaccact
      841 gcgtggtggc gcggagaatc accggcgtct tatgccgcca gcaatgcatg tagctgtgcg
      901 tgtgcttcca gtctacggcg agcagcgcac cccgctcctc gatggccttg acgatcttcg
      961 agttggcgtc ccagatggag agcccgccaa agagcgcgag cgatggtgcg aagcggccgt
     1021 cgcccatcac cggcgcgagg atgtcggcgt cgcgcatgcc gtagcgccgg caggattcga
     1081 aatcctcgac gccgtaggcg ggcgccgagt gcacgatccc ggtgccggtt tcgagggtga
     1141 cgtactcgcc gagataaacc ggcgcctcgc ggtcgtagaa cggatggcgg aagcgaacgt
     1201 tctcgagcgc tcggcctttg cactgcgcta ctgtccttcc cgaaaggcca tagcgcttca
     1261 agcaggcgtc tttcaggtcg gtggcaagga tcagcaggcc cctgtcggtg tcgacgaggt
     1321 cgtagctgaa atcgggatga acgttcagcg cctggttggc gggcaacgtc cacggcgtcg
     1381 tggtccagat caccgcgcag gccgttttgt tgatcgggac gttgaacgcc gctccaagct
     1441 gttgcacatc ccggccggaa accgggaaag ccacatcgac cgcggggtcc ctgcggtcct
     1501 cgtactcgac ttccgcctcg gcgagcgccg agccgcagtc gaagcaccag ttcaccggct
     1561 tgagcccgcg ggagacgtac cccttctcca gcagccggcc gagcacacga atctcttgcg
     1621 cctcggtgcg cggcgccatg gtggtgtacg gctggtccca gtcgccgagc actccgaggc
     1681 gcccgaaatc cttcttctgc cgctcgatct gctcggtggc gaaagcgcgg cacaggcgca
     1741 gcgtttcctc cgtcggcagg ttcttgccgt acttcttctc gatctgcacc tcgatcggca
     1801 tgccgtggca gtcccagccc gggacataag gcgcgtcgaa cccggccagc gtcttcgact
     1861 tcaccacgat gtccttcagg atcttgttca ccgcggtgcc gatgtgaatg tcgccgtttg
     1921 cgtacggcgg cccgtcgtgc agcacgaagc gcggccggcc gttggcgagg ccgcgcagcc
     1981 ggcggtagac gcctttctcc tgccattgct tgacccactg cggctcgcgc cgggcgaggt
     2041 cgccgcgcat tggaaacggc gtttccggaa ggttgagcgt gtctttgtag tcagccatgg
     2101 ctcagaaaga attcgcgcgc ctgcctcgcg tcgcgcgcga tcgcggcctg cagcgcagga
     2161 aggccgtcgt acttctgctc atcgcgcagc ttcttcagga agcgcacgcg caggtgctcc
     2221 ccgtagagat cgctatcgtg gtcgaacaag tgcacctcca gcagcggaat cgcgaccggg
     2281 ttgatcgtcg ggcgccggcc gatgctcgcg accccgcggc cgtatttgtc cacctcgacg
     2341 acgaagatgc cggcaagcgg cggcgcgcgc cgcagcacga tattggcggt cgggaagccg
     2401 agctcgcgcc cgagccgctc gccgtgggcc acgcgcccgc tcatcgagta gcgccggcca
     2461 agcagccgct cggcccgggc aagatccccg gccgccag
//
LOCUS       DASUNT010000183         3483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_23606, whole genome shotgun sequence.
ACCESSION   DASUNT010000183 DASUNT010000000
VERSION     DASUNT010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23606"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VFX94_03575"
     CDS             complement(<1..574)
                     /locus_tag="VFX94_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767292.1"
                     /translation="MAEGSFRRTILVLQGLLITLVVGYVLDLQRSVLKLNLYTEQFLL
                     AVLGLAIALCFLMTCRHRIVDAAAALAGLGICLYLAVRYPQLSTELTSRPLDGILTSA
                     ALALLVLEGTRRMAGFSLVGFTLAGVLYALWGHNLPGVFQARPVEFTRLLVYLGLDTN
                     ALLGTSLQIGIIVVIPFILMGQVLSRCGGAE"
     gene            complement(580..1545)
                     /locus_tag="VFX94_03580"
     CDS             complement(580..1545)
                     /locus_tag="VFX94_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX5767293.1"
                     /translation="MKRILGSLLAALLVPAAWLVPCAAQAQVLGFGSAPQGSIGYNMS
                     SAIAKVLAEDAKLQSRVQPYSGSSAVLPLVNSGELDLAVCNVLEIEEATRGEGPYNGR
                     KQEKLRVLAVIFPLLSSIFVREDSPIKSLADLKGKRIPAGFSAQVTLDRIIDAVLANG
                     GVERKDIVPVLVPNVIRGADDFVEGKIDGGFFALGAAKVLEVHKSTGGIRYLPVSDDP
                     TAVARMKKHMAYAYVTTVNPGPAFAGVATPTKLMAYDYLVTVGSHVKDDQVYRIAKAM
                     YEGKPKLIQSLGAFRGFNPDLMNRAMPATFHPGAMKFYQEKGIAK"
     gene            complement(1558..1947)
                     /locus_tag="VFX94_03585"
     CDS             complement(1558..1947)
                     /locus_tag="VFX94_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5767294.1"
                     /translation="MIERVDHIVLTTRDKDACIRFYSVVLGMDLVRFKTPTEERLALR
                     FGSQKINLHEWGKEFTPRAHVAVPGSLDLCFIASVPLEAVIERLDKAKIRIIEGPVAK
                     TGAQGPIRSVYVRDPDLNLVEISVYNS"
     gene            complement(1944..2375)
                     /locus_tag="VFX94_03590"
     CDS             complement(1944..2375)
                     /locus_tag="VFX94_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl dehydratase"
                     /protein_id="HEX5767295.1"
                     /translation="MKLVGRGFAFDDLKVGFRFRTHRRTISEADRAAFVSLSWLTEEL
                     FAVDEEHKPRLVPGALVYSFAEGLLLPTMQDTGLAFLNATLDVKAPTVVGDTIHVECE
                     VLESRPASKGERGLVRFANRVVNQRGETVLEYNPLRLLKKT"
     gene            complement(2372..2632)
                     /locus_tag="VFX94_03595"
     CDS             complement(2372..2632)
                     /locus_tag="VFX94_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767296.1"
                     /translation="MISRDEYVQKLKEQIDHWNAQAAKWESANAKHVEQFRARRDEAL
                     AELRRVQSASANAWRDMMRGADSALKGVQEAFDKARKQSDKK"
     gene            2847..3392
                     /locus_tag="VFX94_03600"
     CDS             2847..3392
                     /locus_tag="VFX94_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767297.1"
                     /translation="MPPEAGAGAVGLPDPIPVDPGVVGLVVVPVELELPVLPEAPLPA
                     LEPLRASRSHFSFSAPVRARHLLASLPDAPDAAPAEPELPDVELSLLPVDEPLADGVL
                     ELDGVLALPPELAPEDGEAEGVLAPPDAPAPVLPEDAPLLPLLEPEDCAKATPESARS
                     AAAVAAVRVFNIMERSPSKGG"
BASE COUNT          530 a         1166 c         1198 g          589 t
ORIGIN      
        1 actcggcccc gccgcagcgc gacagcacct gccccatcag gatgaacggg atgacgacga
       61 tgatcccgat ctgcaacgag gtgccgagga gcgcgttggt gtcgaggccg aggtagacga
      121 gcaggcgcgt gaactcgacc ggccgcgcct ggaacacgcc gggcaggttg tggccccaca
      181 gcgcgtacag cacgcctgcg agcgtgaagc cgacgagcga gaagcccgcc atgcggcgcg
      241 tgccttcgag cacgaggagg gcgagggccg ccgaggtgag gatgccgtcg agcgggcgcg
      301 aggtgagctc ggtcgaaagc tgcgggtagc gcaccgcgag gtacagacag atccccaggc
      361 cggcgagcgc cgccgccgca tcgacgatcc ggtgcctgca ggtcatcagg aagcacagcg
      421 cgatcgccag tcccagcacc gcgagcagga actgctcggt gtacaggttc agcttgagaa
      481 cgctgcgctg caggtcaagg acgtagccca ccaccagcgt gatgagcagc ccctggagta
      541 cgaggatggt tctccgaaaa gaaccctcgg ccattccggc tacttggcga tgcctttttc
      601 ttggtagaac ttcatcgcgc cgggatggaa ggtggccggc atggcgcggt tcatcaggtc
      661 gggattgaag ccgcggaagg cgccgaggct ctggatcagc ttcggcttgc cctcgtacat
      721 ggccttggcg atgcgataca cctggtcgtc cttcacgtgc gagccaaccg tcaccaggta
      781 gtcgtaggcc atcagcttgg tcggcgtggc gacgccggcg aaggccgggc ccgggttcac
      841 cgtggtcacg taggcatagg ccatgtgctt tttcatgcgc gccaccgccg tcgggtcgtc
      901 ggagaccggc aggtagcgga tcccgccggt cgacttgtgc acctccagca ccttggccgc
      961 gccgagcgcg aagaagccgc cgtcgatctt gccctcgacg aagtcgtcgg cgccccggat
     1021 cacgttgggc accagcaccg gcacgatgtc cttgcgctcc accccgccgt tggcgagcac
     1081 cgcgtcgatg atgcggtcga gcgtgacctg cgccgagaag ccggccggga tgcgcttgcc
     1141 cttcaggtcg gcgagcgact tgatgggcga gtcctcgcgc acgaagatgc tcgacagcag
     1201 cggaaagatc accgccagca cgcgcagctt ttcctgcttg cgcccgttgt aggggccctc
     1261 gccgcgcgtc gcctcctcga tctcgagcac gttgcacacc gccaggtcga gctcgcccga
     1321 gttgaccagc ggcagcaccg cgctcgagcc cgagtagggc tgcacccggc tctgcagctt
     1381 ggcgtcctcg gcgagcacct tggcgatcgc cgagctcatg ttgtagccga tcgagccctg
     1441 cggcgcactg ccgaagccga gcacctgcgc ctgcgcggcg cacggaacga gccacgcggc
     1501 cggaacgagc aacgcggcga gcaacgaccc cagaattcgc ttcatggcac tcctcctcta
     1561 gctattgtag acggagatct cgaccaggtt gaggtcggga tcgcggacat acaccgagcg
     1621 gatcggcccc tgcgcgccgg tcttggcgac cgggccttcg atgatccgga tctttgcctt
     1681 gtcgagccgc tcgatgaccg cctccagggg cacgctggcg atgaagcaca ggtcgagcga
     1741 gccgggcacc gcgacgtgcg cgcgcggggt gaactccttg ccccactcgt gcaggttgat
     1801 cttctggctg ccgaacctga gcgcgaggcg ctcctcggtg ggggtcttga agcgcaccag
     1861 gtccatgccg agcaccacgc tgtagaagcg gatgcaggcg tccttgtcgc gggtggtgag
     1921 gacgatgtgg tcgacgcgct cgatcatgtc ttcttcagca ggcgcagcgg gttgtactcg
     1981 agcacggtct cgccgcgctg gttgaccacc cggttggcga agcgcaccag gccgcgctcg
     2041 cccttggacg ccgggcggct ctcgagcacc tcgcactcga catggatcgt gtcgccgacc
     2101 accgtcggcg ccttgacgtc gagcgtggcg ttgaggaagg cgagcccggt gtcctgcatg
     2161 gtgggcagca gcaggccctc ggcgaaggaa tagaccagcg cccccggcac caggcgcggc
     2221 ttgtgttcct cgtcgacggc gaacagctcc tcggtcagcc acgacaggct gacgaaggcg
     2281 gcgcggtcgg cctcgctgat ggtgcggcgg tgcgtacgga agcggaagcc caccttcagg
     2341 tcatcgaagg cgaagccgcg gccgaccagt ttcatttttt atcagactgc ttgcgcgcct
     2401 tgtcgaacgc ctcctgcacg cccttcagcg ccgaatcggc gccgcgcatc atgtcgcgcc
     2461 aggcgttggc cgatgcgctc tgcacccggc gcagctccgc cagggcctcg tcgcgccgcg
     2521 cgcggaactg ctcgacgtgc ttggcgttgg cgctttccca cttcgccgcc tgcgcgttcc
     2581 agtggtcgat ctgctccttg agcttctgga cgtactcgtc gcggctgatc atcgccctcc
     2641 ccctcacgtg cgctgcgaaa gctcagccat tctagcgcgc atggcgcgtg gcttactttt
     2701 cagggtgcgc cgccggccgc gtccgctatt gcggatctgg tgcttttcct tgcgctcggg
     2761 aagcgcggtt gccggggatt acgcggaatc cccggccaaa ccgcagttgc tctgaccagc
     2821 tacttcctta ctgctttttt acatccatgc cgccggaggc tggggccggc gcggtggggc
     2881 tgcccgatcc cattccggtc gacccggggg tcgtcgggct ggtggtcgtg ccggtggagc
     2941 tggagctgcc ggtgctgccc gaggcgccgc tgcccgcgct ggagccgctg cgcgcttcgc
     3001 gctcgcactt ctctttctcc gcgccggtca gggcgaggca cttgctggcg tcgctgcccg
     3061 acgctccgga cgccgcgccg gccgagccgg agctgcctga cgtggagctg tcgctgctgc
     3121 cggtggacga accgctggcc gacggggtgc tcgagctcga cggggtgctc gcgctgccgc
     3181 ccgagctcgc gcccgaggac ggcgaggcgg agggagtgct cgcgccgccc gacgcgcctg
     3241 cgccggtgct gcccgaggac gcgccgctgc tgccgctact ggagcccgaa gactgcgcga
     3301 aagccacgcc ggagagcgcg agaagcgccg ctgcggtcgc tgccgtgagg gttttcaaca
     3361 tcatggagag atctccttcg aagggtggat gaaagctgcg catttgtgca cgcaagcgcc
     3421 gtgccgtcgc ggcgcgaaaa attctcgcta cgccgcggaa tgttttcatt cgggcggcgt
     3481 gct
//
LOCUS       DASUNT010000184         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_987939, whole genome shotgun sequence.
ACCESSION   DASUNT010000184 DASUNT010000000
VERSION     DASUNT010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987939"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFX94_03605"
     CDS             <1..292
                     /locus_tag="VFX94_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5767298.1"
                     /translation="EHMRYDEDGNNITGSLADYLVATSTDLPNFEVIPMHTPSRGNPV
                     GTKGMAEGGVMGAVGVIMSAVNDALAPFGVVADRQPLSPDYLRSLLRGRSKA"
     gene            complement(302..685)
                     /locus_tag="VFX94_03610"
     CDS             complement(302..685)
                     /locus_tag="VFX94_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5767299.1"
                     /translation="MPKAKQKFVVSRRKESAFKPGFRSYFLDRDLGLREATGGAFMAE
                     VHRACGPVPKGGGGMHLHHINFQFNYMLKGWCRVELEGEGVFRFEEGDTWLQPPAIKH
                     NFLECSPDCEILEICSPGNFETIEV"
     gene            672..1709
                     /locus_tag="VFX94_03615"
     CDS             672..1709
                     /locus_tag="VFX94_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5767300.1"
                     /translation="MAFGILVLARFVGHHTSEENDPAMTVARALREPLARVPALLPAL
                     PGAAYLLALLAAGFALASPEFLSPRNLSNIALQSAALIIVSLGMTLVILTEGIDLSLG
                     AVLGLAGVVMGLLLVSPGTSLVLAICAAVLIGVVAGMCNGVLVAYAAMPPFIVTLGVY
                     GIAQSLAMVLTAGNSVTGLPAAVRWFNEGNLLGMPVPILAMAAVFALTWVLLHRTRFG
                     RYVFAIGGNRRALELAGVPARAWLVAVYAYAGLLAAIASFITTARMNAAHPTIAIGLE
                     FDAIAAVILGGTSFEKGNGGIWGTLVGALAVAVLRNGLNLVGLSTEWQVAVVGLVIVG
                     AVALDALRGHA"
     gene            1706..>2008
                     /locus_tag="VFX94_03620"
     CDS             1706..>2008
                     /locus_tag="VFX94_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767301.1"
                     /translation="MIAAVQRLPAEVAFVVFTRTAFLAALVIVLAIASPHFLTSANMV
                     IVLRQAALLFLMSAGLTLVVLTGGIDLSVGAVLGLAACLGAGFIAHGMVLTGVLLAL"
BASE COUNT          279 a          688 c          649 g          392 t
ORIGIN      
        1 cgagcacatg cgctacgacg aggacggcaa caacatcacc ggctcgctcg ccgactacct
       61 cgtcgccacc tcgaccgacc taccgaactt cgaggtgatc cccatgcaca ccccgagccg
      121 cggtaatccg gtcggcacca agggcatggc cgagggcggg gtgatgggcg ccgtgggcgt
      181 gatcatgagc gcggttaacg acgcgctcgc gcccttcggc gtcgtcgccg accgccagcc
      241 gctcagcccg gattacctgc gctctctatt gagaggacgt tcgaaggcgt gaagctttag
      301 ctcagacttc gatcgtctcg aagttccccg gcgagcagat ctcgaggatc tcgcagtcgg
      361 gcgagcactc gaggaagttg tgcttgatcg cgggcggctg cagccaggtg tcgccttcct
      421 cgaacctgaa cacaccttcg ccttcgagct ccacgcggca ccagcccttc agcatgtagt
      481 tgaactggaa gttgatgtgg tggagatgca tgccgccgcc gcctttcggc acggggccgc
      541 acgcgcgatg cacctctgcc atgaacgcgc cgccggtcgc ctcgcgcagg ccgagatcgc
      601 gatcgaggaa gtaactgcgg aagccgggct tgaacgcgct ctccttgcgc cggctgacga
      661 cgaacttctg tttggctttc ggcatccttg tcctcgcgcg cttcgtcggt catcatacaa
      721 gtgaggagaa cgacccggca atgaccgtcg cgcgcgcgtt gcgcgagccg ctcgccagag
      781 tgcccgcgct tctgcccgcg cttcccggcg ccgcgtattt gctcgcgctc ctcgccgcag
      841 gcttcgcgct cgcgagcccc gaattcctct cgccgcgcaa tctgtccaac atcgcgctgc
      901 agtcggcggc gctcatcatc gtctcgctcg ggatgacgct cgtaatcctc acggaaggca
      961 tcgacctcag cctcggcgcg gtgctcggtc tggcgggcgt ggtgatgggg cttcttctcg
     1021 tttcaccagg cacatccctt gtgctcgcca tttgcgctgc agtcctgatc ggtgtggtgg
     1081 ccggaatgtg caatggggtg ctggtcgcct acgcggcaat gccgccgttc atcgtaacgc
     1141 tcggtgtcta cggcatcgca cagagcctcg cgatggtgct gaccgccggc aattcggtta
     1201 ccggactgcc cgccgcggtg cgctggttca acgaagggaa cctgctcggc atgccggtgc
     1261 cgattcttgc catggccgcc gtgttcgcgc tcacttgggt gctgctccat cgcacgcggt
     1321 tcgggcgcta cgtgttcgcg atcggcggta accggcgcgc gctcgagctc gccggcgtgc
     1381 cggcgcgcgc ctggctcgtc gcggtctacg cgtacgcggg cctgctcgcc gcgatcgcct
     1441 cgttcatcac gaccgcgcgc atgaacgccg cgcacccgac catcgcgatc ggccttgagt
     1501 tcgacgccat cgcggcggtg atcctcggcg gaacctcgtt cgaaaaagga aacggcggca
     1561 tctggggcac gctcgtcggc gcgctcgccg tggcggtatt gcgcaatggc ctgaacctcg
     1621 tcggcctgag caccgaatgg caggtggctg tcgtcggctt ggtgatcgtc ggcgctgtcg
     1681 cgctcgatgc gctgagaggg cacgcatgat cgcggcggtg cagcgcctgc cggcggaggt
     1741 cgcgttcgtt gtcttcaccc gcacggcgtt cctcgccgcg ctcgtcatcg tccttgcgat
     1801 cgcgagcccg catttcctga cgagcgccaa catggtcatc gtgctgcgcc aggcggcgct
     1861 gctcttcctc atgtccgccg gactgacgct cgttgtgctc acgggcggca tcgacctctc
     1921 cgtcggcgcc gtgctcgggc ttgccgcctg cctcggcgcg ggcttcatcg cccacggaat
     1981 ggtattgacg ggagtcttgc ttgcgctg
//
LOCUS       DASUNT010000185         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_725309, whole genome shotgun sequence.
ACCESSION   DASUNT010000185 DASUNT010000000
VERSION     DASUNT010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725309"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VFX94_03625"
     CDS             <1..633
                     /locus_tag="VFX94_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5767302.1"
                     /translation="LYGMFAARGKVVGEPLAEGGRLGEYFIRQPSDPYREYALKWDGE
                     WQVTYETFRDMGLAYAVGLILIYLLVVAQFGSYLTPLVIMAPIPLTVIGVMPGHALFG
                     ANFTATSMIGMIALAGIIVRNSILLVDFVNLKLAEGMPLAEAVKRSAGARAKPILLTA
                     LAAMLGALFILDDPIFNGLAISLIFGIAVSTLLTLVVIPVLFYALMARSD"
     gene            697..1086
                     /locus_tag="VFX94_03630"
     CDS             697..1086
                     /locus_tag="VFX94_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEX5767303.1"
                     /translation="MSKYAFGKTVNMNFEQAVGAVTQALAKEGFGVLTEIDVQATLKK
                     KLGLDRPAYKILGACNPQFAAKALEAEPQIGALLPCNVVVRNDAAGKTIVEFMDPDAV
                     LKLVQHPEVGKIAAEVRSRLERVMAAL"
     gene            1083..1703
                     /locus_tag="VFX94_03635"
     CDS             1083..1703
                     /locus_tag="VFX94_03635"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5767304.1"
                     /translation="MSLEELERWNQRYGEADYIFGTAPNAFLARHAGLFKPGQRALCV
                     ADGEGRNSVWLAEQGLEVVAFDFSPVAVEKARRLAAGRGVQVRYELAAVYDWPWPEAA
                     FDVVAAIFVQFADPPMRAFLFERMIAALKPDGLLLIEGYTPAQLKYGTGGPKQLDQLY
                     TEELLRQAFGSLDILELRTYEAELDEGIRHRGISAVIDLVARRSLK"
     gene            1799..2380
                     /locus_tag="VFX94_03640"
     CDS             1799..2380
                     /locus_tag="VFX94_03640"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX5767305.1"
                     /translation="MRITLLQGHPDPSGGHFCHALCDAYAQYASDAGHELNTIDVGRL
                     GFPLLRSADDWKQGEPVPAIRAAQEAIAWAEHLVIFFPLWLGDMPALLKGFLEQTLRP
                     GFAIGKAGRSRMPRKLLKGRSARIVVTMGMPAFFYSVYYRGHSVKSLKRNVLEFCGFS
                     PVRTSLVGTVEGSSRTAHELWLAKMGTFGRAGT"
BASE COUNT          433 a          863 c          827 g          443 t
ORIGIN      
        1 ctctacggca tgttcgcggc ccgcggcaag gtggtgggcg agccgctcgc cgaaggtggc
       61 cggctcggcg aatacttcat ccgccagccc tccgacccct atcgcgaata cgcgctcaag
      121 tgggacggcg aatggcaggt gacctacgag accttccgcg acatggggct cgcgtacgcg
      181 gtcggcctca tcctcatcta tctcctcgtc gtcgcgcagt tcggctcgta cctcacgccg
      241 ctcgtcatca tggcgcccat cccgctcacc gtgatcggcg tcatgcccgg gcacgcgctc
      301 ttcggcgcca acttcaccgc cacctcgatg atcggcatga tcgcgctcgc cggcatcatc
      361 gtgcgcaact ccatcctgct ggtggatttc gtgaacttga agctcgccga aggcatgccg
      421 ctcgccgagg cggtcaagcg ctcggccggc gcgcgcgcga agccgatctt gctcaccgcg
      481 ctcgctgcga tgctcggggc gctgttcatt ctcgacgatc cgatcttcaa cggcctcgcc
      541 atctcgctca tcttcggcat cgcggtgtcg acgctcctta ccctcgtcgt catcccggtc
      601 ttgttttacg cccttatggc gagatcagat taattcgttg gcctgcttct tcgcgcaggc
      661 gaaccatcca cagaacctct atcaccaggt gacctcatga gcaaatacgc attcggcaag
      721 acagtgaaca tgaacttcga gcaggcggtc ggcgcggtca cgcaggcgct ggcgaaggaa
      781 ggtttcggcg tcctcaccga gatcgacgtg caggccacgc tgaagaagaa gctcggcctc
      841 gaccggccgg cctacaagat actcggcgcc tgcaacccgc agttcgccgc caaggcgctc
      901 gaggccgagc cgcagatcgg cgcgctgctt ccatgcaacg tggtggtgcg caacgacgcg
      961 gccggcaaga ccatcgtcga gttcatggac cccgacgcgg tgctgaagct ggtgcagcac
     1021 ccggaggtcg gcaagatcgc cgccgaggtg cgctcgcggc tggagcgggt gatggcggcg
     1081 ctttgagcct ggaagagctc gagcgctgga accagcgcta tggcgaggcc gactacatct
     1141 tcggcaccgc gccgaacgcg ttcctcgcca ggcacgccgg gctcttcaag ccgggacaac
     1201 gcgcactctg cgtcgccgac ggcgaaggcc gcaacagcgt ctggcttgcg gagcaggggc
     1261 tcgaggtcgt ggcgttcgat ttttcgcccg tggcagtcga aaaggcgcgc cgcctcgcgg
     1321 cgggccgcgg cgtgcaggtg cgctacgaac tcgccgcggt ctacgactgg ccctggccgg
     1381 aagccgcgtt cgacgtcgtg gcggcgatct tcgtgcagtt cgccgacccg ccgatgcgcg
     1441 ccttcctgtt cgagcgcatg atcgcggcgc tcaagccaga cggccttctg ctcatcgagg
     1501 gctacacgcc ggcgcagctc aagtacggca ccggcgggcc gaagcagctg gaccagctct
     1561 acaccgagga gctgctgcgc caggcgttcg gctcactcga catcctcgag ctcaggacct
     1621 acgaggccga gctcgacgag ggaatccgtc accgcggcat atcggcagtg atcgacctcg
     1681 tcgctaggcg gagcctcaag taagcagcag ggccgccgac gcggcgaccc cgcaaccggc
     1741 ctgagcggtc gttgagccaa atcaagcttt acgccgagga cggcgctatc gtgcagcggt
     1801 gaggatcacc ctgctgcagg gccatccgga tccgagcggc ggccatttct gccacgcgct
     1861 ctgcgacgcc tacgcgcaat acgcgagcga cgcgggccac gagctcaata cgatcgacgt
     1921 cgggcggctc ggcttcccgt tgctgcgcag cgccgacgat tggaagcaag gcgagcccgt
     1981 gccggcgatc cgtgcagcgc aggaggccat cgcctgggcc gagcacctgg tcatcttctt
     2041 tccactttgg ctcggcgaca tgccggcgct cctcaagggc ttcctcgagc agacgctgcg
     2101 cccgggtttc gccatcggca aggccggccg ctcgcgcatg ccgcgcaagc tcttgaaggg
     2161 ccgctcggcg cgcatcgtgg tgacgatggg aatgccggct ttcttctaca gcgtctacta
     2221 ccgcgggcac agcgtgaaga gcctcaagcg caacgtcctc gagttctgcg gcttctcgcc
     2281 ggtgcgcacg agcctcgtcg gcacggtgga gggctcgagc cgcacggcgc acgagctttg
     2341 gctcgccaaa atgggaacgt tcggacgagc gggaacgtaa caagccctgt tgacttgcat
     2401 caatacgagt tcttccgtcg gaggtaggct gtgatgaagt tctacacaaa ggagctgaca
     2461 tgagaaagac attgatcgcg tgcgcgctcg cgctcgccac cacggcggtg ttcgcgcaag
     2521 gttacggccg cggccccggc tacggttatg gaccgggcat gatggg
//
LOCUS       DASUNT010000186         2323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_375443, whole genome shotgun sequence.
ACCESSION   DASUNT010000186 DASUNT010000000
VERSION     DASUNT010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375443"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VFX94_03645"
     CDS             complement(<1..502)
                     /locus_tag="VFX94_03645"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5767306.1"
                     /translation="MDFFQAQERARRDSRWLVVWYAAAVLAVVASYCLLAALVYLVVR
                     QSAMPLRGHAWVAALVGGLIVAVSAWRMWQLSEGGMAVAHVLGARYVDPDKCTPAERK
                     LVNVVEEMAIASGISVPPVYLLEFDDAINALVAGYSPHEAVIIATRGAVQKLSRDELQ
                     GVMGHEF"
     gene            complement(504..1100)
                     /locus_tag="VFX94_03650"
     CDS             complement(504..1100)
                     /locus_tag="VFX94_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX5767307.1"
                     /translation="MGTGGWIVLGIVVVLAVWAIVSYNGLVALRNRYKNAFAQIDVQL
                     KRRYDLIPNLVESVKGYMAHERNTLDAVIKARGDALGAAQRAAAAPGDPAAMRGLAQA
                     EGVLGGALGRLLAVFEQYPDLKANQNVLALQEELTSTENKVAFARQAYNDSVMEYNTR
                     RESFPDTIFAGMFGFQAAELLQATESAEERKAPKVSFT"
     gene            1116..>2323
                     /locus_tag="VFX94_03655"
     CDS             1116..>2323
                     /locus_tag="VFX94_03655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5767308.1"
                     /translation="MVSLLYRALRARPGELRAVAWAFAYFFLLLCSYYILRPVRDALA
                     VEVGPQALKWLFTATFVAMLALVPLFGWLCARLPRGRLLPLVYGFFALNLIIFSLYMS
                     ATVFFVWLSVFNLFAVSVFWSFMSDIFDTPQAARLYGAIAAGGSSGAIAGPLVAAFLS
                     RTLEHSGLLFLAAALLALTIPCIAMLSRWSRAHPRSSDPPPGAPLGGSVLAGVRAALG
                     SRFLLAICGYLLCYTALSTALYFQQVAIVKDAVPDMNERTQLFASVDLVVNSLTLALQ
                     VVAFSRLSAVLSAGWLLAVVPLISIAGFIWLGATPTLAALITFGVARRVGEFAISKPA
                     REALFTVVPREERYKAKNFIDTVVYRGGDAASGWLFGALGSAIAFVAAAVAIGWTAIA
                     FYLGARMKSWT"
BASE COUNT          312 a          836 c          732 g          443 t
ORIGIN      
        1 tgaactcgtg ccccatcacg ccctgcagct cgtcgcgcga gagcttctgc accgcgccgc
       61 gcgtcgcgat gatcaccgcc tcgtgcggcg agtagccggc gacgagggca ttgatcgcgt
      121 cgtcgaactc gagcaggtac accggcggca ccgagatgcc ggaggcgatc gccatctcct
      181 cgaccacgtt gacgagcttg cgctcggcgg gcgtgcactt gtccgggtcg acgtagcgcg
      241 cgccgaggac atgcgcgacc gccatcccgc cctcggagag ctgccacatc cgccaagccg
      301 acaccgcgac gatgaggccg ccgacgagcg ccgcgaccca ggcatggccc cgcagcggca
      361 tcgcgctctg cctcacgacg aggtagacca gcgcggcgag caggcagtag ctcgcaacca
      421 cggcaagcac cgcggcggcg taccacacga caagccagcg cgagtcgcgg cgcgcgcgct
      481 cctgcgcttg gaagaaatcc aagctacgtg aacgagacct tcggcgcctt gcgctcctcc
      541 gccgactcgg tcgcctgcag gagctccgcc gcctggaagc cgaacatccc ggcgaaaatg
      601 gtgtcgggaa aggactcgcg cctggtgttg tattccatca ccgagtcgtt gtaggcctgg
      661 cgcgcgaacg cgaccttgtt ctcggtgctg gtgagctctt cctgcagcgc gagcacgttc
      721 tggttcgcct tcaggtccgg gtactgctcg aacacggcga gcagtcggcc gagcgcgccg
      781 ccgagcacgc cttcggcctg cgcgagcccg cgcatcgcgg cggggtcgcc cggcgccgcc
      841 gccgcacgct gcgccgcgcc gagcgcgtcg ccgcgcgcct tgatgaccgc atcgagcgtg
      901 ttgcgctcgt gcgccatgta gcccttcacg ctttcgacca ggttcggaat gaggtcgtag
      961 cgccgcttga gctgcacgtc gatctgcgcg aacgcgttct tgtaccggtt gcgcagcgcc
     1021 accaggccgt tgtagctgac gatggcccag accgccagca cgaccacaat cccaagtacg
     1081 atccatcccc cggtgcccat gcgcctcccc aagtaatggt ttccttacta taccgcgcgc
     1141 tgcgcgcccg gcccggcgag ctccgcgcgg tcgcctgggc gttcgcgtat ttcttcctcc
     1201 ttctttgcag ctactacatc ctgcggccgg tgcgcgacgc gcttgcggtc gaggtcgggc
     1261 cgcaggcgct gaagtggctc ttcaccgcga ccttcgtcgc catgctcgcg ctcgtgccgc
     1321 tcttcggctg gctctgcgca cggctgcctc ggggccgact gctgccgctc gtctacggct
     1381 ttttcgcact gaatctcatc attttcagtc tttacatgag tgccacggtc ttcttcgtgt
     1441 ggctcagcgt cttcaacctg ttcgcggtgt cggtgttctg gagcttcatg tccgacatct
     1501 tcgacacgcc gcaggccgcc cggctttacg gcgccatcgc cgccgggggc agcagcggcg
     1561 cgatcgccgg cccgcttgtg gcggcttttc tttcgcgaac gctcgagcat tccgggctcc
     1621 tttttctcgc cgccgcgctc ctcgcgctga cgattccctg catagcgatg ctttcgcgct
     1681 ggtcgcgggc gcatccgcgc tcgagcgacc cgccgcccgg agcgccgctc ggcggctcgg
     1741 tcctcgccgg cgtgcgtgcc gcgctcggct cgcgtttcct tctcgcgatc tgcgggtacc
     1801 ttctttgcta caccgcgctt tccaccgcgc tttacttcca gcaggtcgcg atcgtgaagg
     1861 acgcggtgcc cgacatgaac gaacgcacgc agcttttcgc cagcgtcgac ctggtggtca
     1921 actcgcttac gcttgccctt caggtcgtcg ctttctcacg tcttagcgca gtcctttccg
     1981 cggggtggct gctcgccgtg gtgccgctca tctccatagc cggcttcatc tggctcggtg
     2041 ccacgccgac gcttgccgcg ctgatcacct tcggcgtcgc gcgccgcgtg ggcgagttcg
     2101 ccatctcgaa gcccgcgcgc gaggcgctct tcaccgtggt gccgcgcgag gagcgctaca
     2161 aggcgaaaaa ctttatcgac accgtggtgt accgcggcgg cgacgcggcg tccgggtggc
     2221 ttttcggcgc gctgggaagc gcgatcgcct tcgtcgcagc ggccgtcgcg atcggctgga
     2281 cggctatcgc gttctacctc ggcgctagaa tgaaatcatg gac
//
LOCUS       DASUNT010000187         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_549956, whole genome shotgun sequence.
ACCESSION   DASUNT010000187 DASUNT010000000
VERSION     DASUNT010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549956"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VFX94_03660"
     CDS             complement(<1..905)
                     /locus_tag="VFX94_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HEX5767309.1"
                     /translation="MTNGTAAYSQKAFTQSLKKYYVIYTGGFIGFVIILAIAEQLGMP
                     NRWIGYAFLIATILLYAIIGVISRTADIAEYYVAGRRVPALFNGMATGADWMSAASFI
                     GMAGSLYLLGYDGLAFILGWTGGYVLVALFLAPYLRKFGQFTIPDFLGARYGGHIPRT
                     IGLLCAILCSFTYVVAQIYGVGIITTRFTGVQLEVGVFLGLAGILVCSFLGGMRAVTW
                     TQVAQYIILIIAYMIPVVMLAYRNHGVPIPHVVYGYTLQKVTEREKQLTNDQKELQVR
                     ETFKKRAADADARIKALPASFSEEKA"
     gene            complement(902..1195)
                     /locus_tag="VFX94_03665"
     CDS             complement(902..1195)
                     /locus_tag="VFX94_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HEX5767310.1"
                     /translation="MQSHYKEERMQLTERHKEYWRKNLTITLILLFIWFVVTFVIGYY
                     ARELHDITILGFPFAFYMGAQGSLVIYIVIIGVYAWYMNKQDKVHGVAEGEEQ"
     gene            complement(1284..1571)
                     /locus_tag="VFX94_03670"
     CDS             complement(1284..1571)
                     /locus_tag="VFX94_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767311.1"
                     /translation="MLTDVVSTVRMMVEICLFSLIGQGVLAVLPGVDRQKNLFYLILK
                     TLASPAWRVARWVSPRFVVDQHVGFVALFILLVFWLGLAVLKQIVTGIESE"
     gene            complement(1575..>2990)
                     /locus_tag="VFX94_03675"
     CDS             complement(1575..>2990)
                     /locus_tag="VFX94_03675"
                     /inference="COORDINATES: protein motif:HMM:NF015410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HEX5767312.1"
                     /translation="QSTARLEQRIAEEQQAFARPLRSLVKRPPVTCAPAISVAEAAAL
                     MHAEEVGSLVVVNDAARAVGIVTNQDIVAALAKNASGQRVADAMTPDPLSLPGHALAY
                     EAAVAMIAQRIRHVLVTEEGRIAGVVSERDLFSLQRLGLGELAMEIRLAAGIEVLAGL
                     AGEVRGLTRTLVEQGVGAEQLTLFVSVLNDRISQRAIEIVRKRHDLERIRWCWLSFGS
                     EGRLEQTFSSDQDNGLLFEVHEAGPQAAARGRLLPFAREVNEALDACGYPLCKGNVMA
                     SNPELCLSLDEWKTKMARWIDVTLPKALLDAAICFDFRPLHGDATLSAALRAWVARRV
                     RAHPAFLRHLAEAALQGRPALGRLGGFATEGGMINLKLHGVKVFVDAARVYALAHGLP
                     QTNTAERLRAASSHIGLRDVAAIADAFYVIQSLRLRIQKDGSENRIAPEKLNRLERAT
                     LKEALRLGRELQERLALDYQL"
BASE COUNT          547 a          952 c          995 g          496 t
ORIGIN      
        1 gccttctctt cgctgaacga ggccgggagc gccttgatgc gggcgtcggc gtcggccgcg
       61 cgcttcttga aggtctcgcg gacctgcagc tccttctggt cgttcgtgag ctgcttctcg
      121 cgctcggtga ccttctgcaa cgtgtagccg tacacgacat gcgggatcgg cacgccgtga
      181 ttgcggtagg cgagcatcac caccgggatc atgtaggcga tgatgagaat gatgtactgc
      241 gccacctggg tccacgtgac cgcgcgcatg ccgccgagaa acgagcacac caggatgccg
      301 gcgaggccga ggaacacgcc gacctcgagc tgcacgccgg tgaaccgggt ggtgatgatg
      361 ccgacgccgt agatctgcgc caccacgtag gtgaacgagc acaagatcgc gcacagcagg
      421 ccgatggtgc gcgggatgtg gccgccgtag cgcgcgccga gaaagtcggg aatcgtgaac
      481 tggccgaact tgcgcaggta aggcgcgagg aagagcgcca ccagcacgta gcccccggtc
      541 cagcccagga tgaaggcgag gccgtcgtag ccgaggaggt acagcgatcc ggccatgccg
      601 atgaaggagg ccgcgctcat ccagtcggcg ccggtcgcca taccgttgaa aagcgcaggc
      661 acgcgccggc cggcgacgta gtactcggcg atgtccgcgg tgcggctgat cacgccgatg
      721 atggcgtaga gcaggatcgt cgcaatgagg aaggcgtagc cgatccagcg gttcggcatg
      781 ccgagctgct cggcgatcgc caggatgatg acgaagccga tgaagccgcc ggtgtagatg
      841 acgtagtact tcttgagcga ctgggtgaag gccttctggc tgtatgctgc ggttccgttg
      901 gtcattgttc ttctccttcg gctacgccgt ggaccttgtc ctgcttgttc atgtaccagg
      961 cgtacacacc aatgatcacg atgtagatga ccaaggagcc ctgcgcgccc atgtaaaagg
     1021 cgaacgggaa gccgaggatc gtgatgtcgt gcaattcacg cgcgtaatag ccgattacga
     1081 aagtgacgac gaaccaaatg aagagcagaa tcaatgtgat cgtcagattc ttgcgccagt
     1141 actccttgtg tcgctcggtg agctgcatgc gctcctcctt gtagtgagac tgcaccaccc
     1201 ctgataaggc ccacgcatcg tggcgccaaa tacttaccac aaacttacag cgcctaatgc
     1261 gcgtggtttc gtgctgcgct gcgtcattca ctctcgatgc cggtcacgat ttgcttgagc
     1321 acggcgaggc ccagccaaaa cacgagcaga atgaagagcg cgacgaagcc cacgtgctga
     1381 tcgacgacaa agcggggcga tacccagcgc gcgacgcgcc acgcgggcga cgcgagcgtt
     1441 ttgagaatca ggtagaagag gtttttctgg cggtcgacac ccggcagcac cgcgagcacg
     1501 ccctgcccga tgagcgagaa caggcaaatc tcgaccatca tccgcacggt ggaaacgaca
     1561 tcggtcagca tggctcagag ctggtagtcg agggcgagcc gctcctgcag ctcgcgcccg
     1621 agccgcagcg cttccttgag agtagcgcgt tccaggcggt tcagcttttc cggcgcgatc
     1681 cggttctccg agccgtcttt ctggatgcga agccgcaggc tctggatcac gtagaacgcg
     1741 tctgcgatcg cggcgacgtc gcgcaggccg atatgtgaag acgccgcgcg cagtcgctcg
     1801 gcggtgttgg tctgcggcag tccgtgcgcg agcgcgtaga cgcgcgcggc atccacgaac
     1861 actttcactc cgtggagctt gagattgatc atcccgccct cggtggcgaa accgccgagc
     1921 cgcccgagcg ccggtctgcc ctgaagcgcg gcttccgcca ggtggcgaag gaacgcagga
     1981 tgcgcgcgca cccggcgggc gacccacgcg cgcagcgcgg ccgaaagcgt ggcatcgccg
     2041 tggagcgggc ggaaatcgaa gcatatcgcg gcgtcgagca gcgccttcgg cagcgtgacg
     2101 tcgatccagc gcgccatctt ggtcttccac tcgtcgagcg agaggcaaag ctccgggttc
     2161 gacgccatca cgttgccctt gcatagcggg tagccgcagg cgtcgagcgc ctcgttgacc
     2221 tcgcgcgcga agggcaacag ccggccccgc gcagcggcct gcgggccggc ttcgtgcacc
     2281 tcgaagagca ggccgttgtc ctggtcggag ctgaaggtct gctcgaggcg cccctcgctg
     2341 ccgaacgaga gccagcacca gcggatgcgc tcgaggtcgt ggcgcttgcg tacgatctcg
     2401 atcgcgcgct ggctgatgcg atcgttgagc accgagacga acagcgtgag ctgctccgcg
     2461 cccacgccct gctccaccag cgtgcgcgtc agcccgcgca cctcgccggc gagcccggcc
     2521 agaacctcga tgcccgcggc gaggcggatc tccatcgcaa gctcgcccag gcccagccgc
     2581 tgcagggaga agagatcgcg ctccgatacc acgccggcga tcctgccctc ctcggtgacg
     2641 agcacgtggc ggatgcgctg cgcgatcatc gccaccgcgg cttcgtaggc gagcgcgtgt
     2701 cccggcagcg acaacggatc gggcgtcatc gcgtcggcca cgcgctgacc ggaagcattt
     2761 tttgcgagcg ctgcgacgat gtcctggttg gtgacgatgc cgaccgctcg cgcggcgtcg
     2821 ttcacgacca cgagcgatcc gacctcctcg gcgtgcatga gcgccgccgc ttctgccacc
     2881 gagatcgctg gcgcgcaagt gaccgggggg cgctttacca gcgaacgtag cggccgcgcg
     2941 aaagcctgct gctcttcagc gatgcgctgc tcgaggcgcg cggtcgattg
//
LOCUS       DASUNT010000188         2782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1164456, whole genome shotgun sequence.
ACCESSION   DASUNT010000188 DASUNT010000000
VERSION     DASUNT010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164456"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..>2782)
                     /locus_tag="VFX94_03680"
     CDS             complement(76..>2782)
                     /locus_tag="VFX94_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5767313.1"
                     /translation="LRLSEERYARAMEGSEAGHWDWNIVTDEMFVSERAREMLALPAG
                     TLPARRAEIMALVPQHPADRDAMHLNVARGIESGYHERDYRIIPRPGEMRWLRSRGRV
                     YKDAGGTAVRMTGSLIDITERRLAADALRASEERYSLAMEASEEGHFDWNVRTDEIFV
                     SAHIKKVFGLPPDAEFRTRNELVARIAYHPDDRPWLDESAPRILCGTALARDFEYRVL
                     RAGEVRWLRARWKIFRDDCGAALRVIGVVTDITDRKRAEEELRDSEARFRALTQLSSD
                     WYWQQDENLRFTYLSGQVQDLTGYSGESSFGKLRWEIANMTPLSCSWAEHQAALAARQ
                     PFRDLECRRVGPDGTVRYLSISGAPIFDAQGNFRGYQGIGRNITERKHIEEALRQSEE
                     RYALAVAGSNEGIFDWDLVSDRVYVSHRAQELFGLPRGELWRPRRDWRHILAFHPDDA
                     PRLHDSIKSHIEGGAPTYDVEFRIVMRGGGVRWFRQRGIALRDTVGKAYRVVGSIGDV
                     TEQHLAEEELRNLERKLRQAQRLEAMGTLAGGIAHDFNNILGAILGYGEMALRDAPKG
                     SRLRRDLDSIITAGERGRALVDRVLAFSRSGVGERVAVHVEKVVREALDLVSARLPES
                     VTLHAKLHAGPAAMLGDATQVHQVLMNLTSNAVQAMPAGGTLRVSLDTVRVDCARATT
                     IGNVVAGDYVVLKVADTGTGIAPEIVERIFDPFFTTKEVGTGTGLGLSLVHGIVSEVG
                     GAIDVASAVGAGSTFTVYLPRSGDAEEILEDKELAMPRGEGQRVLVVDDEEPLVRLAT
                     RTLEELGYAPVGFTSSAAALAAFRADPQRFDALITDERMPGMSGSALVREVRGIRDGI
                     PIVLMSGFIGGRVATLAREAGAEEVLKKPLSARDLAASLARVLHS"
BASE COUNT          390 a          999 c          957 g          436 t
ORIGIN      
        1 cggtcaccgc cgcgtgcgcg ggacccagga tgtgatcggc ggcgccgacc gcagccgcga
       61 gctcgaaggg ccggctcacg agtgcagcac ccgcgcgagg ctcgcagcga ggtcgcgcgc
      121 cgacaacggc ttcttcagca cctcttcggc gcccgcctcg cgggcgaggg tcgcgaccct
      181 gcccccgatg aagccgctca tcagcacgat cgggatgccg tcgcgaatgc cgcgtacctc
      241 gcgaacgagg gccgagcccg acatgcccgg catgcgctcg tcggtgatga gcgcgtcgaa
      301 gcgctgcgga tcggcgcgga acgcggcgag cgccgcggcg ctcgaggtga atccgaccgg
      361 cgcatagccc agctcctcga gcgtgcgcgt ggcgagcctt accagcggct cctcgtcgtc
      421 caccaccagc acgcgctggc cctctccccg cggcatggca agctctttgt cttcgagaat
      481 ctcctcggcg tcgccgctgc gcggcaggta gacggtgaac gtgcttccgg ccccgaccgc
      541 gctcgcgacg tcgatcgcgc cgccgacctc gctcacgatg ccgtgcacga gcgagagccc
      601 gaggccggtg ccggtgccga cctccttcgt cgtgaagaac ggatcgaaga tccgctcgac
      661 aatctcgggc gcaatgccgg tgccggtgtc ggcgaccttc agcactacat agtcccccgc
      721 caccacgttg ccgatggtcg tcgcgcgcgc gcagtccacg cgcaccgtgt cgagcgatac
      781 gcgcagcgtc ccgcccgcgg gcatcgcctg gacggcgttg ctcgtgaggt tcatcagcac
      841 ctggtgcacc tgcgtggcgt cgccgagcat ggccgcgggc ccggcgtgca gcttggcgtg
      901 cagcgtcacg ctctccggca gccgggccga gacgagatcg agcgcctcgc gcaccacctt
      961 ttccacgtgg accgccacgc gctcgccgac gccgctgcgg ctgaaggcga gcacccggtc
     1021 caccagcgcg cgcccgcgct cgcccgcggt gatgatgctg tcgaggtcgc gccgcaggcg
     1081 gctgcccttg ggcgcgtcgc gcagcgccat ctcgccgtag ccgaggatcg cgccgaggat
     1141 gttgttgaag tcgtgcgcga tgccgcccgc aagcgtgccc atcgcctcga ggcgttgcgc
     1201 ctggcgcagc ttgcgctcga ggttcctcag ctcttcctcg gccaagtgct gctcggtcac
     1261 gtcgccgatg gagccgacca cccggtacgc cttgccgacg gtatcgcgaa gcgcgatgcc
     1321 gcgctggcgg aaccagcgca ccccgccgcc gcgcatcacg atcctgaatt ccacgtcgta
     1381 cgtaggcgcg ccgccctcga tgtgcgactt gatgctgtcg tgcaggcgcg gcgcgtcgtc
     1441 ggggtggaag gcgagaatgt ggcgccagtc gcggcgcggc cgccacagct cgccgcgcgg
     1501 caggccgaac agctcctgcg cccggtgcga gacgtagacg cggtcgctca ccaggtccca
     1561 gtcgaagatg ccttcgttcg agcccgccac tgcgagcgcg tagcgctcct ccgactggcg
     1621 cagcgcctcc tcgatgtgct tgcgctcggt gatgttgcgc ccgatgccct ggtagccccg
     1681 gaaattgccc tgcgcgtcga atatcggcgc gccgctgatg ctgagatagc gcacggtccc
     1741 atcgggcccg acgcggcggc actcgagatc gcggaacggc tggcgcgccg ccagtgcggc
     1801 ctggtgctcg gcccaggagc acgagagggg cgtcatgttg gcgatttccc agcgcagctt
     1861 gccgaacgag gattctccgg agtacccggt caggtcctgc acctggcccg agaggtaagt
     1921 gaagcgcagg ttttcgtcct gttgccagta ccagtcggag gaaagctgcg tgagcgcgcg
     1981 gaagcgcgct tcgctgtcgc gcagctcctc ttcggcgcgc ttgcggtcgg tgatgtcggt
     2041 caccacgccg atcacgcgca gcgccgcgcc gcagtcgtcg cggaaaatct tccagcgcgc
     2101 gcgcagccag cgcacctcgc ctgcgcgcag gacgcggtac tcgaaatcgc gggcgagcgc
     2161 ggtgccgcag agaatcctgg gcgcgctctc gtcgagccag ggccggtcgt cgggatggta
     2221 ggcgatgcgg gcgacgagct cgttgcgggt gcggaactcc gcatccggcg gcaagccgaa
     2281 caccttcttg atgtgcgccg agacgaagat ttcgtcggtc ctgacgttcc agtcgaagtg
     2341 cccctcctcc gaggcctcca tcgcgaggct gtagcgctct tcgcttgcgc gcagggcgtc
     2401 ggctgcgagc ctgcgctcgg tgatgtcgat gagggagccg gtcatgcgca ccgcggtccc
     2461 gccggcgtcc ttgtagaccc tgccgcggga gcgcagccag cgcatctcgc ccgggcgcgg
     2521 gatgatgcga tagtcgcgct cgtggtagcc cgattcgatg ccccttgcca cgttgaggtg
     2581 catcgcgtcg cggtcggcgg gatgctgcgg caccaaagcc atgatctcgg ccctgcgcgc
     2641 gggaagcgtg cccgcaggca gggcgagcat ttccctcgcc cgctccgaca cgaacatttc
     2701 gtccgtgacg atgttccagt cccagtggcc cgcctccgag ccctccatcg cgcgcgcgta
     2761 gcgctcctcc gacaggcgca gc
//
LOCUS       DASUNT010000189         2891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1164530, whole genome shotgun sequence.
ACCESSION   DASUNT010000189 DASUNT010000000
VERSION     DASUNT010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164530"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VFX94_03685"
     CDS             <1..354
                     /locus_tag="VFX94_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008345871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX5767314.1"
                     /translation="FGHPVYTIADPRNEVIKAVAKRLSRNACDKLFAIAERLEQVMWR
                     EKKMFANLDWYSAVSYHAMGVPTDMFTPLFVISRTSGWAAHVIEQRADGKIIRPSANY
                     TGPENRPWVPLGSRA"
     gene            complement(351..800)
                     /locus_tag="VFX94_03690"
     CDS             complement(351..800)
                     /locus_tag="VFX94_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5767315.1"
                     /translation="MYKHILLPTDGSRLAGKGAAEGVRLAKALGARVTGIYVAAPFYR
                     PPTYLEGGVYIPGIPPKVYRKHSEARAKKALAPIEAAARKARVRCATRMLTAPRASDG
                     ILKAARALRCDAIAMSSRCRRGFDAVLLGSETRRVLLSSKVPVVVFR"
     gene            883..1335
                     /locus_tag="VFX94_03695"
     CDS             883..1335
                     /locus_tag="VFX94_03695"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX5767316.1"
                     /translation="MRDKLLRQDTLGGLVIIAVAAFAYWQGRDLSTAAAGGMGPGTLP
                     KGLAVLLGGLGVLLVLHSFKSVRMGRWSVRGPLFVVGALVLFGIAVRPHGLAVAGPLA
                     IAAASFASPEVRWRETLIYGVLMTLFCVALFKYALGLPIPLAPWLVGY"
     gene            1337..2836
                     /locus_tag="VFX94_03700"
     CDS             1337..2836
                     /locus_tag="VFX94_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5767317.1"
                     /translation="MELLSNLALGFATALSPTNLFLGFIGCLVGTLIGVLPGVGPIAT
                     IAMLMPLTLKVDPTGALIMLAGIYYGAQYGGSTTAILVNMPGEVTAVVTALDGHEMAK
                     QGRAGAALGIAALGSFFAGTIATLVIAAIGVPLTKLALLFGPAEYFALMVFGLGLAIV
                     LARGSVLKAVIMVVLGLLLSTVGTDLETGQERLTLGIPELSDGVDFAVLAMGLFGIAE
                     ILRNLENPESRNVAIAQIGRLLPTRAELKRCFGPVVRATGVGSILGILPGNGAVLGPF
                     ASYAMEKKLAKDPSRFGKGAIEGVAGPEAANNAGAQTSFIPLLTLGIPANPVMALMIG
                     AMIIQGIVPGPNVASEQPALFWGIIASMWIGNLMLIILNLPLIGIWVRLLSVPYRLLY
                     PAILLFCVIGIYATNTNFAQIVMTAVFAVFGYILIKFDCEPAPLVLGFILGPLMEENL
                     RRSLVISRGDPMVFLERPISATLLALTAIIIVLIVLPQIRKSREEAFKE"
BASE COUNT          433 a          984 c          937 g          537 t
ORIGIN      
        1 ttcggccacc cggtctacac catcgccgat ccgcgcaacg aggtgatcaa ggccgtcgcg
       61 aagcgccttt caaggaatgc ctgcgacaag ctcttcgcga ttgccgagcg gctggagcag
      121 gtcatgtggc gcgagaagaa gatgttcgcc aatctcgact ggtacagcgc ggtgtcgtac
      181 cacgcgatgg gcgtgccgac cgacatgttc acgccgctct tcgtcatctc gcgcacctcg
      241 ggctgggccg cgcacgtgat cgagcagcgc gccgacggca agatcatccg gccgagcgcg
      301 aattacaccg gtccggaaaa ccggccctgg gtaccgctcg gcagccgggc ctagcgaaag
      361 acgacgaccg gaaccttgga gctgagcagg acgcggcgcg tttcgctgcc gaggagcaca
      421 gcgtcgaagc cgcggcggca gcgcgaggac atcgcgatcg catcgcagcg cagcgcgcgc
      481 gcagccttga ggatgccgtc gcttgccctg ggcgccgtca gcatgcgggt cgcgcagcgc
      541 acgcgggcct tcctggcggc cgcctcgatc ggggcaagcg cctttttcgc ccgcgcttcg
      601 ctgtgcttgc gataaacctt cggtgggatg ccggggatgt acacgccgcc ttccaggtag
      661 gtgggcggcc ggtaaaacgg cgccgcgacg tagatgccgg ttaccctcgc gccgagcgcc
      721 ttggcgagcc gcacaccttc ggccgcgcct ttgccggcga gcctggagcc gtccgtcgga
      781 agcaggatat gcttgtacat ggaggaatcg tgcccgcgct cgccgtgagg cctattgacg
      841 tggatcaaaa tcgctggcca atgtgttaga agaatggcgc aaatgcgcga caagctcctg
      901 aggcaggata cgctcggcgg tctcgtgatc atcgcggtcg ccgcgttcgc ctactggcag
      961 gggcgcgacc tgtcgaccgc cgccgcgggc ggcatggggc ccggaacgct gcccaaaggc
     1021 ctcgcggtgc tgctcggcgg cctcggcgtg ctgctcgtgc tgcattcgtt caagagcgtg
     1081 cgcatgggcc gctggtcggt gcgcggcccg ctcttcgtgg tgggcgcgtt ggtgcttttc
     1141 ggcatcgcgg tgcgcccgca cggcctcgcg gtcgccggcc cgctcgcgat cgcggccgcg
     1201 tcattcgcct cgcccgaagt gcgctggcgc gagacgctga tctacggcgt gctgatgacg
     1261 ctcttctgcg tcgcgctctt caagtacgcc ctggggcttc ccattccgct cgctccctgg
     1321 ctcgtcggct attgatatgg agctcttgag caatctcgct ctgggcttcg cgacggcgct
     1381 ttcccccacc aatcttttcc tcggattcat cggctgcctc gtcggcacgc tgatcggcgt
     1441 gctgcccggc gtcggcccga tcgccaccat cgcgatgctg atgccgctca cgctgaaggt
     1501 tgacccgacc ggcgcgctca tcatgctcgc cggcatctac tacggcgccc agtacggcgg
     1561 ctccactacc gcgatcctcg tcaacatgcc gggcgaagtc accgcggtgg tcaccgcgct
     1621 cgacggccac gagatggcga agcaggggcg cgccggagcg gcgctgggca tcgccgcgct
     1681 cggctcgttc ttcgccggca cgattgccac gctcgtcatc gcggcgatcg gcgtgccact
     1741 caccaagctc gcgctgctct tcgggcctgc cgagtacttc gccctcatgg ttttcggcct
     1801 cgggcttgcc atcgtgcttg cgcgcggctc ggtgctgaag gcggtgatca tggtggtgct
     1861 cggccttctt ctctccacgg tgggcaccga cctcgagacc ggccaggagc gtctcacgct
     1921 cggcatcccg gagctttccg acggcgtgga cttcgccgtg ctcgcgatgg ggcttttcgg
     1981 catcgccgag atcttgagga acctcgagaa ccccgaatcg cgcaacgtgg cgatcgccca
     2041 gatcgggcgc ctgctgccga cgcgggcgga gctgaagcgc tgcttcggcc cggtcgtgcg
     2101 cgccaccggc gtgggatcga ttctgggcat cctgcccggc aacggcgccg tgctcggccc
     2161 cttcgcttcc tacgccatgg agaagaagct ggcgaaggat ccgtcgcgct tcggcaaggg
     2221 cgcgatcgaa ggcgtcgccg ggccggaagc cgccaacaac gccggcgcgc agacctcgtt
     2281 cattccgctg ctcaccctcg gcattcccgc caatccggtg atggcgctga tgatcggcgc
     2341 gatgatcatc cagggcatcg tgcccggtcc gaacgtcgca agcgaacagc ccgccctctt
     2401 ctggggcatc atcgcctcga tgtggatcgg caatctgatg ctgatcatcc tcaacctgcc
     2461 gctgatcgga atctgggtgc ggttgctctc ggtgccgtac cgcctgcttt atccggcgat
     2521 cctgctgttc tgcgtcatcg gcatctacgc caccaacacc aacttcgccc agatcgtcat
     2581 gaccgcggtg ttcgcggtgt tcggctacat cctgatcaag ttcgactgcg agcccgcgcc
     2641 gctggtgctc ggcttcatcc tcgggccgct gatggaggag aatttgcgcc gctcgctcgt
     2701 catctcgcgc ggcgacccga tggtcttcct cgagcggccg atctcggcga cactgctggc
     2761 gctgaccgcg atcatcatcg tgctgatcgt tttgccgcaa atccgaaagt cgcgcgagga
     2821 ggctttcaag gaataggcac tgcccgccac aatcaaacaa aagaaatacc tgggaggacg
     2881 cacatgacca t
//
LOCUS       DASUNT010000190         4711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1164571, whole genome shotgun sequence.
ACCESSION   DASUNT010000190 DASUNT010000000
VERSION     DASUNT010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164571"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VFX94_03705"
     CDS             complement(<1..648)
                     /locus_tag="VFX94_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5767318.1"
                     /translation="MRVAIPDLVSPSYFPLIAAVELGFFAKQGLRASLELLYPVTKTY
                     DELCEGRLDFVGGASHAALYAFKDWEGGRLLCALAQYMYWFLVVRSDLEAKRGDVRVV
                     RSLRIGAAPGPVDGLKQLLKRAGIDPAKDVQIGPVPGVVEKTASFGVMAAKALEDGKL
                     DGFWANGMGAEVAVRRGIGTVVVDARRDGSQEVKGYTFPALVCTEKTIKENPDAAR"
     gene            complement(645..1598)
                     /locus_tag="VFX94_03710"
     CDS             complement(645..1598)
                     /locus_tag="VFX94_03710"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5767319.1"
                     /translation="MRIRIGLAFALAAAASLAQAQQWPQRPIKVVVPFPPGGVTDSIA
                     RITADWLAPRLAQPVVAENRPGASGAIAAEFVARSEPDGYTLLSAATPQLAVVPHVQK
                     ISYDPVRDFAPISIVGTNAFALGCNDKIPATSLSEFVAYVKQRQGELSFASAGTGSVG
                     HLTMALFLSRAGLKMEPVLYKGGGPAMADVVAGHVPCYFGNLNELIPHAGGGRIRVIA
                     VSGGQRAPQLPQVPTVAEQGFPGFRTVTWNGYVAPAATPREVIERIAREIGAACKDAA
                     FAERLNKIGVDPLCGTPGDFTQAIRDDLATWKEAVQAAGMK"
     gene            1639..2814
                     /locus_tag="VFX94_03715"
     CDS             1639..2814
                     /locus_tag="VFX94_03715"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5767320.1"
                     /translation="MQRRNVIVVGAGPVGTVAALACARRGLSVTLLEAQTRIDDSPRA
                     STTQPPTLEILAELGLIDEYLRVGLVSRTFQFWDRPKRRLIAEFDFDRLKDETAYPFV
                     VQTEQHKLANMAIARLSGMANAEVRMGSRVVGVVQDENQASVQTAENTYAADYAIAAD
                     GGRSAVRKALDIEFEGYTWPERFLVITTKFDFQAALGCCLRNYMADPREWTNLFKVAG
                     DDLKGRWRAVFNTREEETDEEALSDAAVRARLSRVYVPEASREYLHLNLYAVHQRVAK
                     SFRKGRVFLCGDAAHVNNPIGGLGLNSGIHEAWHLAELLSRASRHEPVDLDAYERTRR
                     PLNIKYVQEQTVANKKRLEERDPAARERRFRELGEMADDPRRHKAFLMRASLLESSR"
     gene            complement(2780..>4711)
                     /locus_tag="VFX94_03720"
     CDS             complement(2780..>4711)
                     /locus_tag="VFX94_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dehydroadipyl-CoA semialdehyde
                     dehydrogenase"
                     /protein_id="HEX5767321.1"
                     /translation="QQCRGQAHAQIGGENAGKAARDTAEKSMLIGTAIGAAIGGLFGG
                     AEGAALGAVFGLGGGAVVGSDATYSAGASLQRRYDNAFTLCMYEKGHKVPVPAGSRYP
                     NGSQSQPATRTPPPPPPPGQPPPEAPPDYRPKSYPGMLELRSYVGGRWVAGTGPVQTL
                     LDPTTEEPLARASSEGVDLESALHYARSVGGPALRAMSFAERGAALKAIADAIQGSRD
                     ALLEFAIANGGNTRSDAKFDIDGAIATLLAYAEISSSLGSSRFLVDGEGIQLGRSARF
                     HGQHILVPRRGVAVHINAFNFPAWGFAEKAAVALLAGMPVLTKPATSSAMVAHRVIEL
                     IVEGKLLPEGAVSFLVGGAGDLVSRLASQDVLAFTGSGETARKLRSARNIIEHSVRVN
                     VEADSLNAAVLGPDVQPRSDMYELFLKDVVRDMTQKAGQKCTAIRRILVPEEVASAVR
                     DDLAELLGGIHVGDPKLEDTRMGPLASAAQLADVREGVQRLGGKLLFGGIAPLAPKGF
                     FISPVLVDEPGEAVHEREVFGPVASVLRYTESPAELVARGAGGLVSSIYSEDQSFVES
                     VAGELAPYHGRIYLGHPKIELAPGPGTVLPQLVHGGPGRAGGGEELGGARGLAFYMQR
                     VALEGSRPVIAKILGAKPA"
BASE COUNT          794 a         1599 c         1575 g          743 t
ORIGIN      
        1 acgagcggcg tccgggtttt ccttgatcgt cttctcggtg catacgagcg cgggaaacgt
       61 gtagcccttc acctcctggg agccatcgcg ccgcgcatcc acgactacag taccgatgcc
      121 acggcgaacg gcgacctccg cgcccatgcc gttcgcccag aagccgtcga gcttgccgtc
      181 ctcgagcgcc ttcgcggcca tgacgccgaa cgaagccgtt ttttccacca cccccggcac
      241 ggggccgatc tgcacgtctt tcgccggatc gattcccgcg cgcttgagaa gctgcttcag
      301 cccgtcgacc ggtccgggcg cggcgccgat tcgcagactt ctcacaacac gcacatcgcc
      361 tcgctttgct tccagatcgg aacgcacgac gaggaaccag tacatgtact gggcgagcgc
      421 gcagagcagc cgcccgccct cccagtcctt gaatgcatag agggccgcgt gcgacgctcc
      481 accgacgaag tcgagccggc cttcgcacag ctcgtcgtac gtcttggtga ccggatagag
      541 cagttccaac gaagcgcgga gcccctgctt tgcaaaaaac ccgagttcca cggccgcgat
      601 gagcgggaag taagacggcg agacgaggtc ggggatcgcg accctcattt cattcccgcc
      661 gcctgcacgg cttccttcca cgtcgcgaga tcgtcccgga tcgcctgggt gaagtcgccc
      721 ggcgtgccgc acagcgggtc gacgccgatc ttgttgagcc gctcggcgaa ggccgcatcc
      781 ttgcacgccg cgccgatctc gcgcgcgatg cgctcgatta cctcgcgcgg cgtcgcggcg
      841 ggggcgacat agccgttcca ggtcaccgtg cgaaacccgg gaaagccctg ctcggcgacc
      901 gtaggaacct ggggaagctg cggcgcgcgc tgcccgccgg agactgcgat cacgcggatt
      961 ctgccgccgc ctgcgtgcgg gatgagctcg ttgaggttgc cgaagtagca gggaacgtgg
     1021 ccggcaacga cgtccgccat ggccgggccg ccgcccttgt agagcaccgg ctccatcttc
     1081 aaaccggcac gcgaaaggaa aagcgccatt gtgagatgac cgaccgagcc tgtccccgcc
     1141 gaggcgaagc tgagctcgcc ttgccgctgc ttcacgtagg cgacgaactc ggacaggctt
     1201 gtcgcgggaa tcttgtcgtt gcaccccagc gcgaaagcgt tggtaccgac gatggagatt
     1261 ggcgcgaagt ccctcaccgg gtcgtaggaa attttctgaa cgtgcggcac gaccgcgagc
     1321 tgcggcgtgg ccgccgacaa aagcgtgtag ccgtcgggct cggagcgcgc gacgaactcg
     1381 gcggcgatcg cgccgctcgc gccgggcctg ttctcggcga ccaccggctg cgcgaggcgt
     1441 ggggcgagcc agtcggcggt gatgcgcgca atggaatcgg tgacgccgcc gggcgggaag
     1501 ggcaccacca ccttgatcgg ccgctgcggc cactgctgtg cctgtgcaag ggacgcggcg
     1561 gcggcgagcg caaacgccag cccaatccgg attctcatcg cgagcctcct ccggggctca
     1621 gtataatcgc gccgaaaaat gcagcggcga aacgtcatcg tggtcggcgc gggcccggtc
     1681 ggcacggtgg cggcgctggc atgcgcacgc cgcgggctga gtgtcacgct gctcgaggcg
     1741 cagacccgca tcgacgacag cccgcgcgcc tccaccacgc agccgccgac gctcgagatc
     1801 ctcgccgagc tcggcctcat cgacgaatac ctgcgggtag gcctcgtctc gcgcaccttc
     1861 cagttctggg accgtccgaa gcgcaggctc atcgccgagt tcgatttcga ccgcctgaaa
     1921 gacgagaccg cgtatccgtt cgtagtgcag acagagcagc acaagctcgc caacatggcg
     1981 atcgcgcggt tgagcggcat ggcgaacgcg gaagtgcgca tgggatctcg cgtcgtcggc
     2041 gtggtgcagg acgaaaacca ggcgagcgta caaaccgccg agaacaccta tgcggccgac
     2101 tacgccatcg ccgccgacgg cgggcgtagc gccgtccgaa aggcgctcga catcgagttc
     2161 gagggttata cctggccgga gcgcttcctc gtcatcacca cgaagttcga tttccaggcc
     2221 gcgctgggct gctgcctgcg aaactacatg gccgacccgc gggagtggac caaccttttc
     2281 aaagtcgcgg gcgacgacct caagggacgc tggcgcgcgg tgttcaatac gcgcgaggaa
     2341 gaaaccgatg aagaggcgct atccgatgca gcggtgcgcg cgcgtctttc gcgcgtctac
     2401 gtgcccgagg caagccgtga gtacctgcac ctcaacctct acgccgtgca ccagcgcgtg
     2461 gcaaagagct tccgcaaggg ccgcgtgttc ctttgcggcg acgccgcgca tgtcaacaac
     2521 ccgatcggcg gccttggcct gaacagcggc atccacgagg catggcatct ggcggaactc
     2581 ttgagccgcg cttcgcgcca cgagccggtc gatctcgatg cctacgaaag gacgagacga
     2641 cccctgaaca tcaagtatgt gcaggagcag acggtcgcga acaagaagcg cctggaggag
     2701 agggaccctg cggcgcgcga gcgccgtttc cgcgagctcg gcgagatggc cgacgacccg
     2761 cgacgccaca aggcgtttct tatgcgggct tcgctcctag aatcttcgcg ataacggggc
     2821 gcgagccctc gagcgcaacg cgctgcatat agaacgcgag gccgcgcgcg ccgccgagct
     2881 cctcgccgcc gccggcgcgt cccgggccgc cgtgcacgag ctgcggcagc accgtgcccg
     2941 ggccgggcgc aagctcgatc ttcggatggc cgaggtaaat gcggccgtgg taaggcgcga
     3001 gctcgcccgc cacactttcg acaaagctct ggtcctcgga gtagatcgag gacaccaggc
     3061 cgcccgcgcc tcgcgccacc agctcggcgg ggctttccgt gtagcgaaga acggaggcga
     3121 ccggaccgaa aacctcgcgc tcgtgcaccg cttcgccggg ttcgtcgacg aggacaggag
     3181 agatgaagaa gcccttgggg gcaagcggcg cgatgccgcc gaagaggagc ttcccgccga
     3241 ggcgctgcac gccttcgcgc acgtcggcga gctgcgcggc gctcgcgagc ggccccatgc
     3301 gggtgtcctc gagcttcggg tcgcctacgt ggatcccgcc aagcagctcg gcgagatcgt
     3361 ctctgacggc agaagcgact tcttcgggga ccaggatccg cctgatggcc gtgcacttct
     3421 ggcctgcctt ctgcgtcatg tcgcgcacca cgtccttgag gaagagctcg tacatatcgc
     3481 tgcgcggctg gacgtccggc ccaagcacgg ccgcgttcag gctgtcagct tcgacgttca
     3541 ctcgcacgga atgctcgatg atgtttcttg cggaccgcag cttccttgcg gtttcgcccg
     3601 agccggtaaa agcaaggacg tcctgcgagg cgaggcggga cacgagatcg cccgcgccgc
     3661 cgacgaggaa tgaaaccgcg ccttcgggca ggagcttgcc ttccacgatc agctcgatca
     3721 cccggtgcgc gaccatggcg gagctcgtcg ccggcttggt gagcaccggc attccggcga
     3781 gcagcgctac cgccgccttt tcggcgaatc cccaggcggg gaagttgaac gcgttgatgt
     3841 gcacggcaac gccgcggcgc ggaacgagga tgtgctgccc atgaaagcgc gcggagcgcc
     3901 cgagctgaat gccttcgccg tcgacgagga accggctgga acctagagac gagcttattt
     3961 ccgcgtacgc gagcagcgtg gcgatcgcgc cgtcgatgtc gaacttggcg tccgagcgcg
     4021 tgttgccacc gttcgcgata gcgaactcga ggagcgcatc gcggctcccc tgaattgcgt
     4081 ccgcgatggc tttgagcgcc gcgccacgct cggcgaagct catcgcgcgc agcgcgggcc
     4141 caccgaccga gcgggcgtag tgaagggcgg actcgaggtc cacgccttcg ctgctcgcgc
     4201 gcgcgagcgg ctcttcggtc gtcggatcga gcagcgtttg caccgggccc gttccagcta
     4261 cccagcgccc gccgacgtag ctgcgcagct ccagcatccc gggatatgac ttcggccggt
     4321 agtcgggggg cgcttccggg ggcggttgtc caggcggcgg cggtggcggc ggcgtgcgcg
     4381 tcgccggctg cgactggctg ccattgggat agcggcttcc cgctggcacg gggaccttat
     4441 ggcccttctc gtacatgcaa agcgtgaagg cgttgtcata gcggcgctga agcgaagctc
     4501 cggcactgta ggtcgcatcg cttccgacca ccgcgccgcc gccgagtccg aacactgcgc
     4561 ccagcgccgc gccctctgcg ccgccgaaga ggccgccgat tgcggcgccg atcgccgtgc
     4621 cgatcagcat gcttttctcc gcggtgtcgc gcgccgcctt gccggcgttt tcaccgccga
     4681 tctgtgcgtg ggcctgcccc cggcattgct g
//
LOCUS       DASUNT010000191         2023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_813714, whole genome shotgun sequence.
ACCESSION   DASUNT010000191 DASUNT010000000
VERSION     DASUNT010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813714"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="VFX94_03725"
     CDS             <1..1483
                     /locus_tag="VFX94_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX5767322.1"
                     /translation="NGVRIADSARALVLHETRLAPAYYFPVEDVRMDLLARTEHKTHC
                     PFKGNASYWSVKVGDKVAENVAWAYEEPYREAEAIRGYLSFYRDKVSLVHEGEEEKSL
                     EKIHANPLASWLVDEGWKAGSSAELAERFLKSLRDAGVPVDRSTVIMPTLHPQIFASV
                     LVWRADTPGIRTVFEPHDILYQPKFADSPFAPIIRGAGGVRRRLEDADCALDFPVVRD
                     LKSEGATDYVAMPFRFSDGQINVMSLTSFAPGGFGSAHLGELYEVLPLLGRLFEVHAA
                     RRTATALLETYLGRHTGERVLKGLVKHGDGEQIHAVIWFCDFRDSTPLSKSMGRQAYL
                     RQLNRFFYCMAGAVLEAGGEVLRYIGDAVLAIFPISGNEDEACERAVRAARLAESRIA
                     EMNAKHPDRAPLRYGIGLHLGTVTYGNIGVPERLEFTVIGAAANEAARVEGMTKDLKK
                     PVLTSAAFAGAYQGGLVSLGKHLLKGLEGEHELFTLPDDPMTG"
     gene            complement(1452..>2023)
                     /locus_tag="VFX94_03730"
     CDS             complement(1452..>2023)
                     /locus_tag="VFX94_03730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5767323.1"
                     /translation="RERIASLAYNDALTGLANRTSLAPALEQAIERTRRNRSKLAGVF
                     MDLDGFKQVNDTYGHAAGDAFLVEVARRLRRGLRASDVVARLGGDEFFVVLEQMQDAP
                     AVERVVSKLAAEVLKPYELFGGVKTRCSASLGVSIFPDDAGDAATLMAHADKAMYAAK
                     RAGKNAFCIFSAGQASNPTTIPSSGHQAA"
BASE COUNT          332 a          647 c          720 g          324 t
ORIGIN      
        1 caacggcgtg cgcatcgccg attcggcgcg cgcgctcgtg ctgcacgaga cgcggctcgc
       61 gccggcgtac tactttcccg tcgaggacgt gcgcatggac ctcctcgcgc ggacagagca
      121 caagacgcat tgcccgttca agggcaacgc gagctactgg agcgtgaagg tcggcgacaa
      181 ggtcgccgag aacgttgcct gggcctacga ggagccgtac cgcgaggccg aggcgatccg
      241 cggctatctg tcgttctacc gggacaaggt ttctttggtg catgaaggcg aagaagaaaa
      301 atcgcttgaa aagatacatg ccaacccgct ggcaagctgg ctggtcgacg agggctggaa
      361 agcgggctcg agcgccgagc tggcggagcg gttcctgaag tcgttgcgcg atgccggcgt
      421 gccggtcgat cgcagcacgg tgatcatgcc cacgctgcac ccgcagatct tcgcctcggt
      481 gctcgtgtgg cgcgccgaca cgccggggat ccgcacggtg ttcgagccgc acgacatcct
      541 ctaccagccg aagttcgccg acagcccatt cgcgccgatc atccgcggcg cgggcggggt
      601 gcgccgcagg ctggaggacg cggactgcgc actcgacttc ccggtggtgc gcgacctgaa
      661 aagcgaaggc gccactgact acgtggcgat gccgttccgc ttctccgacg gccagatcaa
      721 cgtgatgtcg ctaacttcct tcgcgcccgg cggcttcggc agcgcgcacc tcggcgagct
      781 ctacgaggtg ctgccgctcc tcgggcggct cttcgaggtg cacgcggcgc ggcgcacggc
      841 cacggcactc ctcgagacct acctcgggcg gcacacgggc gagcgggtgc tcaaggggct
      901 ggtgaagcac ggcgacggcg agcagatcca cgcggtgatc tggttctgtg acttccgcga
      961 ctcgacgccg ctttcgaaga gcatgggccg gcaggcgtac ctgcggcagt tgaaccgctt
     1021 tttctactgc atggcgggcg cggtgctcga ggcgggcgga gaagtgctgc gctacatcgg
     1081 cgatgccgtc ctcgcgatat tcccgatatc cggcaacgag gacgaggcct gcgagcgcgc
     1141 ggtgcgcgcg gcgaggctag cagagtcgag gattgctgaa atgaatgcaa agcatccgga
     1201 ccgtgcgccg ctgcgctacg gcatcggcct gcatctcggc acggtgacct acggcaacat
     1261 cggcgtgccg gagcggctgg aattcaccgt gatcggcgcg gcggcgaacg aggcggcacg
     1321 cgtcgagggc atgaccaaag atttgaagaa gccggtgctg acctcagccg cgttcgctgg
     1381 cgcttaccag ggaggactcg tttctttggg aaaacacctc ctgaaaggcc tggagggcga
     1441 gcacgaactc tttacgctgc ctgatgaccc gatgacggga tagtggtcgg gttgctcgcc
     1501 tggccggcgg agaagatgca gaacgcgttc ttgccggcgc gcttggccgc gtacatggcc
     1561 ttgtcggcat gcgccatgag ggtcgcggcg tcgccggcgt cgtcggggaa gatgctcacg
     1621 ccgaggctcg ccgagcagcg cgtcttgacc ccgccgaaga gctcgtaggg cttgagcacc
     1681 tcggccgcga gcttggacac gacgcgctcg accgcgggcg cgtcctgcat ctgctcgagc
     1741 accacgaaga actcgtcgcc gccgaggcgc gccacgacgt cgctcgcgcg caggccgcgg
     1801 cgcaggcgcc gcgcgacctc gaccaggaac gcgtcgcccg cggcgtggcc gtacgtgtcg
     1861 tttacctgct tgaagccgtc caggtccatg aacacgccgg cgagcttgga gcggttgcgc
     1921 cgcgtgcgct cgatcgcctg ctcgagcgcg ggcgcgaggc tcgtgcggtt ggcgagcccg
     1981 gtgagcgcgt cgttgtaggc gagcgaagcg atgcgctcgc gcg
//
LOCUS       DASUNT010000192         3175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_24256, whole genome shotgun sequence.
ACCESSION   DASUNT010000192 DASUNT010000000
VERSION     DASUNT010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24256"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFX94_03735"
     CDS             complement(<1..546)
                     /locus_tag="VFX94_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HEX5767324.1"
                     /translation="MELTAEERGILAGSRGEAARRALEYQIEVGRFWGAKRFVSVTNV
                     HMMGDIEVMGDAGLEFLRNEVKSAARCAVPTTTNARCIDFAHCERLGQDAAEVAKERE
                     IIQCLRRMDVVTTDTCINYQSVYQPHLGEHVAWGDTGTVIYANSVFGARSNFESGPAA
                     LAAALTGRTPEYGFHLSQNRKG"
     gene            complement(547..885)
                     /gene="fdxA"
                     /locus_tag="VFX94_03740"
     CDS             complement(547..885)
                     /gene="fdxA"
                     /locus_tag="VFX94_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008528408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEX5767325.1"
                     /translation="MTYVVTEDCLKCKYMDCVEVCPVDCFYEGANMLVIHPDECIDCG
                     VCEPECPPKAILPDSDSKAEKWLKLNAEYSVKWPNITRKGTAPPDADAWKDVPGKFEK
                     YFSPEPGKGE"
     gene            1006..2964
                     /locus_tag="VFX94_03745"
     CDS             1006..2964
                     /locus_tag="VFX94_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5767326.1"
                     /translation="MPRQTHLAPLSTLLALVGAALLILTAGTEIIIAARAWLAGEIPL
                     PKEIILHVLVAAAAVLLSAAVIMTRGSARRTAAAEAARRESEERLRLVANNVPALISY
                     VDRDQRYRFSNRTYDDWFGIAHERMQGRTVAEVFGEEAYAHMRPDIERCMAGAAVEFE
                     FATTEGGKPRTLQVACVPHLSDESEVLGFYMLANDVTALKRAQEDLRFAAAQLQYDAQ
                     RLEFLAHHDTLTGLPNRVMFADRAREAVAHARRHAKTAAFLFLDMDNFKQVNDRLGHE
                     VGDELLKVIAARLRSSVRGDDFIARIGGDEFCVLLQEIAEPREAAAVAQKLIHELGKP
                     YLVGEHEVRSGASIGIACVPQDGDDVNRLLRLADHAMYRAKELGRNGYQFFSGLLNDN
                     AEAAAELAEELRAAAERGELFLAYQPRFDIATRQVVGAEALLRWRHPRHGVLAPEAFL
                     HLADDMGLLAELGGWIVREACLQGRRWLDAGVRPFSVVVNASARQLRSGNLAAQARAA
                     LEVSGLPPESLLIEIPETAVRQLAEPIEQSLAEITASGARLSVDDFGTGYASLPMLQK
                     LRASAVCIDRSLLSGVSHDSDRAGLARALIGLARGLNFEVVAKGVETQAQREFLADAG
                     CRICQGDLFAPAGPAHEVEPFLRERHAA"
BASE COUNT          497 a         1122 c         1051 g          505 t
ORIGIN      
        1 tccctttctg ttttgactta aatggaatcc atattcaggg gtgcggccgg tgagcgcggc
       61 ggcaagcgcg gccggcccgg actcgaagtt cgagcgcgcg ccgaacaccg agttcgcgta
      121 gatcacggtg ccggtgtcgc cccaggcgac gtgctctccg aggtgcggct ggtataccga
      181 ctgatagttg atgcaggtgt cggtggtgac gacatccatg cgccgcagac attggataat
      241 ttcgcgttcc ttcgccacct ccgccgcgtc ctgtccgagg cgctcgcaat gcgcgaaatc
      301 gatgcagcgc gcgttcgtgg tcgtcggcac ggcgcagcgc gccgcgctct tcacctcgtt
      361 cctgaggaac tcgaggccgg cgtcgcccat cacctcgatg tcgcccatca tgtggacgtt
      421 ggtaacggaa acgaagcgct tcgcgcccca gaagcggccg acctcgatct ggtactcgag
      481 cgcgcgccgc gctgcttcgc cgcgcgagcc ggcgaggatg ccgcgctcct cggccgtgag
      541 ctccaactac tcgcctttac cgggctctgg agagaagtac ttctcgaact tgcccgggac
      601 gtctttccac gcgtccgcgt ccggcggcgc ggtgcctttg cgcgtgatgt tcggccattt
      661 caccgagtac tcggcgttga gcttcaacca cttctccgcc ttcgagtcgg agtccggcag
      721 gatcgccttc ggcgggcact caggctcgca cacgccgcag tcgatacact cgtcgggatg
      781 gataacgagc atattggcgc cctcgtagaa gcaatccacg gggcacacct cgacgcagtc
      841 catgtacttg cacttgaggc agtcttcggt gacgacgtaa gtcataggcc gcgcattata
      901 aaggcagcac gtcggaaagc ggcgacgcca caatagcggt ttcagcgaca gcgctgcaac
      961 aagttcaacc gttgccgcga cttgcgtcgg gggcgatcat aggcgatgcc tcggcagacc
     1021 catctggcgc cgctcagcac gctcctcgcg ctcgtcggtg cggcgctgct gatccttacc
     1081 gccggcacgg aaatcattat cgccgcgcgt gcctggctcg cgggcgagat cccgctgccg
     1141 aaggaaatca tcctccatgt gctcgtcgcg gcggcggcgg tgctgctgtc cgcggcggtg
     1201 atcatgacgc gcggctcggc gcggcgcacc gcagcggccg aggcggcgcg gcgggagagc
     1261 gaggagcggc tgcgcctcgt ggcgaacaac gtcccggcgc tcatttccta cgtcgaccgc
     1321 gaccagcgct accgcttcag caaccgcacc tacgatgact ggttcggcat cgcgcatgag
     1381 cgcatgcagg gccgcaccgt cgccgaggtg ttcggcgagg aagcgtatgc gcacatgcgc
     1441 ccggacatcg agcgttgcat ggcgggcgcg gcggtggaat tcgagtttgc caccaccgaa
     1501 ggcggaaagc cgcgcacgct gcaggtcgcc tgcgtgccgc acctcagcga tgaaagcgag
     1561 gtactcggct tctacatgct cgccaacgac gtcaccgcgc tcaagcgcgc gcaggaggac
     1621 ctgcgcttcg ccgccgcgca gctgcagtac gacgcgcagc gcctggagtt cctggcccac
     1681 cacgacacgc tgaccggcct tcccaaccgc gtgatgttcg ccgaccgcgc acgcgaggcg
     1741 gtggcgcacg cgcgccgcca cgccaagacc gcggcgtttc tgttcctcga catggataac
     1801 ttcaagcagg tcaacgatcg cctcggccac gaggtcggcg acgagctgct caaggtgatc
     1861 gccgcccgcc tgcgctcgag cgtgcgcggc gacgacttca tcgcgcgcat cggcggcgac
     1921 gagttctgcg tgctgctgca ggagatcgcc gagccgcgcg aggcggccgc ggtggcgcag
     1981 aagctgatcc acgagctcgg taagccgtac ctggtcggcg agcacgaggt gcgctcaggc
     2041 gcgagtatcg ggatcgcgtg cgtgccgcag gacggcgacg atgtcaaccg gctgctgcgg
     2101 ctcgccgatc acgccatgta ccgtgcgaag gagctcgggc gcaacggcta tcagttcttc
     2161 tccggcctgc tcaacgacaa cgccgaggcc gcggccgagc tcgccgaaga gctgcgtgcg
     2221 gcggccgagc gcggcgagct cttcctcgcc taccagccgc gcttcgatat cgcgacgcgc
     2281 caggtggtcg gcgcggaggc gctgctgcga tggcgccatc cgcgccacgg cgtgctcgcg
     2341 cccgaggcct tcctgcacct cgccgacgac atgggcctgc tcgccgagct gggcggctgg
     2401 atcgtgcgcg aagcctgcct gcagggccgg cgctggctcg acgccggcgt ccggccgttc
     2461 tcggtggtgg tcaacgcgag cgcgcgccag ctgcgcagcg gcaacctcgc cgcccaggcg
     2521 cgcgcggcgc tcgaggtaag cggcctgccg cccgaatccc tgctgatcga gatccccgag
     2581 accgcggtgc gccagctcgc cgagccgatc gagcagagcc tcgccgaaat caccgccagc
     2641 ggcgcgcgcc tcagcgtcga cgacttcggc accggctacg cctcattgcc gatgctgcag
     2701 aagctgcgcg cgagcgcggt ctgcatcgac cgcagcctgc tcagcggcgt gtcgcacgac
     2761 agcgaccgcg ccggcctcgc gcgcgccctc atcggcctcg cccgcggcct caacttcgaa
     2821 gtcgtcgcca agggcgtcga gacccaggcg cagcgcgagt tcctcgccga cgccggctgc
     2881 cgcatctgcc agggcgacct cttcgccccc gccggccccg cccacgaagt cgagcccttc
     2941 ctgcgcgagc gccacgccgc ctagcgcgcg tcagcagtcc gatcgctctc gagcggtccg
     3001 tcccgtcata ccatctgggg gcccgctcca aggcgaagcg cagaggcata aatgagcgaa
     3061 accagaccat tcggaaagac cggtgtcgct tttgccgcat tagcggcgct tgtgctgtcg
     3121 agcctcgccg cagcgcagcc cgaggccccg ggcgagcctg tcaccattcc gggcg
//
LOCUS       DASUNT010000193         2732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_24508, whole genome shotgun sequence.
ACCESSION   DASUNT010000193 DASUNT010000000
VERSION     DASUNT010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24508"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /locus_tag="VFX94_03750"
     CDS             complement(<1..126)
                     /locus_tag="VFX94_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5767327.1"
                     /translation="MILYVISVGLSVTMGLMGFVNLAHGVFAMAGGFILSTVMGEL"
     gene            complement(191..1366)
                     /locus_tag="VFX94_03755"
     CDS             complement(191..1366)
                     /locus_tag="VFX94_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5767328.1"
                     /translation="MKFLLGAALALSAAAVSAQTIKIGFITSYSGLNGNLGPYMERAV
                     RLYIKQHEKELPPGVKIELITRDDTGPNPDKAKQLAQELIVRDKVNLLAGVIFTPNAL
                     AIAPLATEAKVPFVIMNAGTSVISTRSPYIVRTSFTLWQSSYPLGTWASKKFKTAYTL
                     VSDFGPGHDAETAFSQSFEAGGGKILSKVRVPLQNPDWAAYMQRVKDAKPDALMVFIP
                     AGKTATAVMKNFSDLGLDKAGIKLIGPGDITTEEELPNMGDVALGATTVFHWSAAGDR
                     PANKAFVAAWKKEYGQNETPNFLSVGSWDGTALIFEAIKQQKGKLDPDKTMAIFKGWK
                     FANSPRGPISIDPETRDIIQPEYLREVRKVGGQLANVELETLGEAVKDPWKELQKKK"
     gene            complement(1372..2238)
                     /locus_tag="VFX94_03760"
     CDS             complement(1372..2238)
                     /locus_tag="VFX94_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5767329.1"
                     /translation="MDVAERQRPAAGELCLDHLAHFVPNLDAAAAVWEKLGLKVTPVA
                     VHQVAGKPSGTSNRCVMFEEGYIELLAPTLDTPHATRVRALMKRYDGVHLVCFGTPDA
                     AAEHARLAAHGFEPEPMVKLGRNLETGEAVRFNVVYVPPGKMAEGRVQYCEHLTPQHV
                     WRKGFVNAPCLRSVYVVADNPEEVAARWGRFGGMLPRRSREGKADGLVRLDMARGRVL
                     IGNKKAVAGVLGEVPSAPAIAGYAIACSNPAAFARRCSKAGMKVKGNVVTLPAALGGS
                     WLLESAGKEEDL"
     gene            2237..2647
                     /locus_tag="VFX94_03765"
     CDS             2237..2647
                     /locus_tag="VFX94_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767330.1"
                     /translation="MEVQIAYSTLKLVFAEVGPGPTLEVGPFPEVRIDGEELRAGRGG
                     EVIGCHKRHAWLVRERNFFRLDCPSPVRLHFENEHGECSPVYGPFMHFSCADGIAYGD
                     GVICANLELQTKRWYDHRGRRYWAQLVVKSASAP"
BASE COUNT          441 a          897 c          891 g          503 t
ORIGIN      
        1 gagctcgccc atcaccgtcg agaggatgaa gccgccggcc atggcgaaca cgccgtgcgc
       61 gaggttgacg aagcccatca ggcccatcgt caccgacagg ccgacggaga tgacgtacag
      121 gatcatgccg taagcgacgc cgtggaaggc gacgcttacg acagacgaga ggaattccgc
      181 cagtgtcttg ctacttcttc ttctgcagct ctttccaggg atccttcacc gcctcgccga
      241 gcgtctcgag ctcgacgttg gcgagctggc cgccgacctt gcgcacctcg cgcaggtact
      301 ccggctggat gatgtcgcgc gtctcgggat cgatcgagat cgggccgcgc gggctgttgg
      361 cgaacttcca gcccttgaag atcgccatcg tcttgtccgg gtcgagcttg cccttctgct
      421 gcttgatcgc ttcgaagatc agcgcggtgc cgtcccagga gccgaccgag agaaagttcg
      481 gcgtctcgtt ctggccgtac tccttcttcc acgcggcgac gaaggccttg ttggccggac
      541 ggtcgcccgc cgcggaccag tggaacacgg tcgtggcgcc gagcgccacg tcgcccatgt
      601 tgggcagctc ttcctcggtg gtgatgtcgc ccggcccgat cagcttgatg cccgccttgt
      661 cgaggccgag gtcggagaag ttcttcatca ccgcggtcgc ggtcttgccc gccgggatga
      721 acaccatcag cgcgtcgggc ttggcgtcct tcacgcgctg catgtaggcc gcccaatccg
      781 ggttctgcag gggaacgcgc accttggaga ggatcttgcc gccgcccgct tcgaacgact
      841 gcgagaacgc ggtttccgcg tcgtgcccgg ggccgaagtc gctcaccagc gtgtacgcgg
      901 tcttgaactt cttcgatgcc cacgtgccga gcggatagct cgactgccac agcgtgaagg
      961 aggttcgcac gatatagggc gagcgcgtgc tgatgaccga ggtgcccgcg ttcatgatca
     1021 cgaacggcac cttcgcttcg gtggcgagcg gcgcgatcgc gagcgcgttc ggcgtgaaaa
     1081 tcacgccggc cagcaggttc accttgtcgc gcacgatcag ctcctgcgcg agctgcttcg
     1141 ccttgtccgg gttggggccg gtgtcgtcgc gggtgatgag ctcgatcttc acgccgggcg
     1201 gcagctcttt ctcgtgctgc ttgatgtaga gccgcaccgc gcgctccatg tacggcccca
     1261 ggttgccgtt caggccggag tagctcgtaa tgaagccgat ctttattgtc tgcgccgaga
     1321 ctgcagcagc ggagagggcg agcgccgccc cgagcagaaa cttcacgatc ctcatagatc
     1381 ctcctccttg ccggccgatt ctagcagcca actcccgccg agcgcggcgg gcagggtgac
     1441 cacgttgcct ttcactttca tcccagcctt gctgcagcgc cttgcgaacg ccgccgggtt
     1501 gctgcacgca attgcatagc cggcgatcgc gggagccgag gggacttccc ccaacacgcc
     1561 ggcaaccgcc ttcttgtttc ctatcagtac ccgtccgcgc gccatgtcga ggcggacgag
     1621 cccgtctgcc tttccttccc tactgcgccg cggcagcatg ccgccgaagc gcccccaacg
     1681 cgccgcaact tcctccggat tgtccgcaac gacataaacg ctccgcaggc aaggcgcgtt
     1741 gacgaagcct tttcgccaga cgtgctgtgg ggtcaggtgc tcgcaatact gaacccggcc
     1801 ttcggccatc tttcccggtg gcacgtacac gacattgaag cgcaccgctt cgccggtctc
     1861 gagatttctt ccgagcttca ccatcggctc gggctcgaag ccgtgcgcgg cgaggcgcgc
     1921 atgctccgca gccgcgtccg gcgtgccgaa gcaaacgagg tgcacgccgt catagcgctt
     1981 catcagcgcg cgcacgcgag tcgcgtgcgg ggtatcgagc gtgggtgcca ggagctcgat
     2041 gtagccttcc tcgaacatca cgcagcggtt cgacgtgccc gagggctttc cggccacctg
     2101 gtgcaccgca accggcgtca ccttgagccc gagcttctcc cacaccgctg ccgcggcgtc
     2161 gaggttgggc acgaagtgtg caaggtggtc gaggcagagc tcgcccgcgg cagggcgctg
     2221 gcgttcggcg acgtccatgg aggtgcagat agcatactcg acgctaaagc tggtattcgc
     2281 agaagtgggg ccggggccga cgctggaggt gggacctttt cccgaagtgc ggatcgacgg
     2341 cgaggagctg cgcgccgggc gcggcggcga ggtcattgga tgccacaaga ggcacgcctg
     2401 gctggtacgc gagcgcaatt tcttcaggct ggattgtccg agcccggtgc ggctgcactt
     2461 cgagaacgag cacggcgaat gctcgccggt ctacgggccg ttcatgcact tctcctgcgc
     2521 cgatggcatc gcctacggcg acggcgtgat ctgcgccaac ctggagctcc agaccaaacg
     2581 ctggtacgac catcggggtc ggcgctactg ggcgcagctc gtggtgaaat cggcaagcgc
     2641 gccctaatat tggggccgtg aacaagcccg tagagctcgc gaccctgact ggggcggaag
     2701 cggtggtcga gatgctgcgc gcccacggcg tg
//
LOCUS       DASUNT010000194         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_24597, whole genome shotgun sequence.
ACCESSION   DASUNT010000194 DASUNT010000000
VERSION     DASUNT010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24597"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1287)
                     /locus_tag="VFX94_03770"
     CDS             complement(58..1287)
                     /locus_tag="VFX94_03770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5767331.1"
                     /translation="MGSDPIFLRVALPFLAGYFVSYVYRMVNAVLAPTLAAEFGLSAG
                     GLGLLSSMYFLSFALCQLPMGLAMDRFGPRRVNAVLLLVAAAGGAWFAHAESAAGAMA
                     ARALIGVGVSVALMSSLTAFVLWYPADRLSTMNAIAFSVGMVGAMTVTVPLELLLRVL
                     PWREAFMLVVAANVGVSLVLWLWVPERTAQPRSDTLAQQLSDLKQLLRDRAFLRLAVC
                     LGASQCAAIALQTLWVATWMRDVAGYTPAQVARGLLAVNATMIGGYLVFGRAADALQR
                     RGRSVLRLLAGGVAFSSFCLALIVVGAKSLLVWCAFVAAGTGVVLGYSIATRRYPKAM
                     AGRANTAINVFGFVGMFGGQWGIGLVLDLWPQTASGYAPEAYPWALGMAWVVQLAGLA
                     WLWNGRALFEEPATGAA"
     gene            complement(1291..2061)
                     /locus_tag="VFX94_03775"
     CDS             complement(1291..2061)
                     /locus_tag="VFX94_03775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5767332.1"
                     /translation="MGPPSTLKDRVAWITGGGSGIGLAGAIELARAGAHVVISGRSAE
                     TLKRAEKELRGAGSGEAISLDVADQKEVARAAQAIHKRHGRIDILVNSAGINVPKRHF
                     SNVSVEGWDQIVAINLSGMFYCCHAVLPVMRERRDGIVINISSWFGRYANPLAGPGYS
                     ATKHAVNALTEQLNIEECANGIRATAILPGEVATPILEKRPVPPPPEERARMLQPEDL
                     ARAIVFIATLPPRACVNELIISPTWNRFYLGGLETPKN"
     gene            complement(2033..>2554)
                     /locus_tag="VFX94_03780"
     CDS             complement(2033..>2554)
                     /locus_tag="VFX94_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX5767333.1"
                     /translation="GPEGKGKPFALCESGAMLFYLAAKTGKFLPKDLRKRWQVMQWVM
                     FQMGHIGPMLGQAHHFLGYAPEKIPYAMNRYKNEANRLYGVVERRLNDSKYIACDEYT
                     IADMAIVPWLRAPERQGVNIDEYPALKSWRDAILERPAVKRALQVLAERRRTEMTPEQ
                     RENLFGAAQYAKR"
BASE COUNT          447 a          875 c          849 g          383 t
ORIGIN      
        1 tcgtcctcgg gccggtattc gccgcgtcca tttacgcctc gtacaaggac atctatctca
       61 ggccgcgccc gtagcgggct cctcgaaaag cgcgcggccg ttccacagcc aggcgagacc
      121 ggcgagctgg actacccaag ccatgccaag cgcccaggga tacgcctcgg gagcgtagcc
      181 gctcgcggtc tgcggccaga ggtcgagtac gaggccgatc ccccattggc cgccgaacat
      241 gccgacgaag ccgaacacgt tgatcgcggt gttcgcgcga ccggccattg ccttcggata
      301 gcgccgcgtg gcgatcgaat agccgagcac cacccccgtg ccggccgcca cgaaagcgca
      361 ccacaccaac aggctcttgg cgccaacaac gatcagcgcc aggcagaatg atgagaacgc
      421 cacgccgccg gcaagcaaac gcaggacgct gcgcccgcgg cgctggagcg catcggctgc
      481 gcgcccgaac acgagatagc cgccgatcat cgtcgcattc accgcgagca ggccgcgcgc
      541 aacttgcgcg ggcgtatagc cggcgacgtc gcgcatccag gtcgcgaccc acaaggtctg
      601 cagcgcgatc gccgcgcact ggctcgcgcc aaggcacacc gcgaggcgca ggaaggcgcg
      661 atcgcgcagc agctgcttca gatccgaaag ctgctgggct aacgtgtcgc tgcgcggctg
      721 cgcggtgcgc tccggtaccc atagccaaag caccaggctg acccctacgt tggccgccac
      781 gacgagcatg aaagcctcac gccacggcaa cacgcgcagc agaagctcca aaggaacggt
      841 gaccgtcatc gcgcccacca tgccgacgga aaatgcgatg gcgttcatgg tcgagagccg
      901 gtccgccgga taccagagga cgaaagcggt gagcgacgac atgagcgcga cggacactcc
      961 gacgccgatc agggcgcgcg ccgccatcgc gcccgccgcg ctctcggcgt gcgcgaacca
     1021 cgccccgccc gccgccgcga cgagcagaag aaccgcgttc acccggcgcg gaccgaagcg
     1081 gtccatcgcg agacccatcg gcagctggca caacgcgaag gagaggaagt acatgctgga
     1141 cagcagcccc aggccgccgg cggagaggcc aaactcggcg gcgagcgtcg gcgcaagcac
     1201 cgcgttcacc atgcggtaca catacgagac gaaatagccc gcgagaaacg gcagcgcgac
     1261 gcgcaggaaa atggggtcag accccatttt ctagttcttc ggagtctcga ggccgccgag
     1321 gtagaaacgg ttccaggtcg ggctgatgat gagctcgttc acgcacgcgc gcggcggcag
     1381 cgtggcgatg aagacgatgg cgcgcgcgag gtcctcgggc tgcagcatgc gcgcgcgctc
     1441 ttcaggcggc ggcggcaccg gccgcttctc gagaatcggg gtcgcgacct cgcccggcag
     1501 gatcgcggtg gcgcggatgc cgttcgcgca ttcctcgatg ttgagctgct cggtgagcgc
     1561 gttcaccgcg tgcttggtcg cgctgtagcc cgggcccgcg agcgggttgg cgtagcggcc
     1621 gaaccacgac gagatgttga tgacgatgcc gtccctgcgc tcgcgcatga cgggcagcac
     1681 ggcgtggcag caatagaaca tgcccgagag gttgatcgcg acgatctggt cccagccttc
     1741 gaccgacacg ttgctgaagt gccgcttggg cacattgatg cccgcgctgt tcacgaggat
     1801 gtcgatgcgg ccgtgacgct tgtggatcgc ctgcgcggcg cgcgccactt ccttctggtc
     1861 cgcgacatcg agcgagatcg cttcgccgct accggcgcct cgcagctctt tttccgctct
     1921 cttgagcgtc tctgcgctgc gccccgagat gacgacgtgg gcgccggcgc gggcgagctc
     1981 gatcgcgccg gcgagcccga tccccgagcc gccgccggta atccaggcga ctctatcttt
     2041 tagcgtactg ggcggcccca aagaggttct cgcgttgctc cggggtcatc tcggtgcgcc
     2101 gccgctcggc gagcacctgc agcgcgcgct tgaccgcggg gcgctcgaga atcgcgtcgc
     2161 gccagctctt gagcgccggg tactcgtcga tgttgacgcc ctgccgctcg ggagcgcgca
     2221 gccaaggcac gatggccatg tcggcgatgg tgtactcgtc gcacgcgatg tacttgctgt
     2281 cattcagcct gcgctccacc acgccgtaga ggcggttcgc ctcgttcttg tagcggttca
     2341 tcgcgtaggg gatcttctcc ggcgcgtagc cgaggaagtg gtgcgcctgg ccgagcatcg
     2401 ggccgatgtg gcccatctgg aacatgaccc attgcatcac ctgccagcgc ttgcgcaggt
     2461 ccttcggcag gaacttgccc gttttcgcgg cgaggtagaa gagcatcgcg cccgactcgc
     2521 agagcgcgaa gggcttgccc ttgccttccg ggcc
//
LOCUS       DASUNT010000195         3900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_376797, whole genome shotgun sequence.
ACCESSION   DASUNT010000195 DASUNT010000000
VERSION     DASUNT010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376797"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VFX94_03785"
     CDS             complement(<1..935)
                     /locus_tag="VFX94_03785"
                     /EC_number="1.3.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR02823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acryloyl-CoA reductase"
                     /protein_id="HEX5767334.1"
                     /translation="MERFKAYKLTETPEKKIRAEFVDYTLDQLDPHEVLVRIAYSSVN
                     YKDALAATGKGKILRRPACVGGIDFAGTVVESRDPRFKKGDAVLTASGEYVGVSSDGG
                     YAPYTRVKGDWIAKVPPGLSLWEAMAFGTAGYTAGIAIVRMEHNGLKPAHGPVIVNGA
                     TGGVGSIALAALAKLGYHVVALTGKESETDWLKKLGAKEIMLRQTLNLQKIKPLDKAT
                     WAGAVDNLGGDVLAWMASTMNYGGVIAAVGLAASHTLNTTVLPFILRGVSLLGVNSGD
                     VSNPIRDLVWRRLATDLKPPLLKEMARTLRFSELPS"
     gene            complement(938..1888)
                     /locus_tag="VFX94_03790"
     CDS             complement(938..1888)
                     /locus_tag="VFX94_03790"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5767335.1"
                     /translation="MRTLVALGLAVACGLGLAQSYPVKPVRLIIPFPPGGSNDVVGRS
                     IGMQLGERLGQNVVIDNRGGAGSIIGTNEAAKAAPDGYTLLLVSTAFPTSIAFNRLPR
                     DAMKSFVPVAMLGSGPALLVVPASSAANSVTDLLNLLKQKPGELNAAAAGIGSFQHMA
                     TELFRLQSKTNFVIVQYKGGGPALTDVIAGQVQMNLGSLVQMIPHVRSGKLKALGVSS
                     AKRVAALPDVPTIAEAGVPGFEVSNWWGILAPAGTPEPALDRLYKEIAAILDSPETRK
                     RFELEGAEVVRLKPQEFATFVTQETEKWTRVVKEAGIKPE"
     gene            complement(1885..2958)
                     /gene="zapE"
                     /locus_tag="VFX94_03795"
     CDS             complement(1885..2958)
                     /gene="zapE"
                     /locus_tag="VFX94_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEX5767336.1"
                     /translation="MVAFYEQSLARRGFVSDSSQWRAVERLQRLHEEWSAYKRRRSSA
                     LRRLLVKPPLPKGMYLWGPVGRGKSFLMDAFYLCVPLVRKRRVHFHHFIREIHRELDA
                     LKGAQDPIAEVAARTARRYRLVCFDEFHVSDIADAMILGRFLEQVMDRGVQFVMTSNY
                     HPEQLYPGGLQRERFVPAIELIKSRLDVVAVDSGTDYRRLKMKQVQVYHLSDAPLARI
                     FEELKDVQEEWHPLDVDGRTIPYRKRAGGVVWFDFHTLCGGPRSYADYVDLAKRFHTV
                     VLSGVPRMSARQSDAARRFTWLVDVLYDERVKLVVSAGAAPEELFVEGQNSADFQRTV
                     SRLHEMQSAEYRLLPARRVALAA"
     gene            complement(2969..>3900)
                     /locus_tag="VFX94_03800"
     CDS             complement(2969..>3900)
                     /locus_tag="VFX94_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5767337.1"
                     /translation="NVNVLDNAGALAIPTLPETIGVVGAGVIGLEMGSVWRRLGAQVT
                     VLEALPAFLGAADEAVAKEAQKVFTKQGLAIHLGVNITGVTTGKDGVEVAWTDPKRAA
                     QTARFDKLIVSIGRIPLSDSLNARSVGLTLDERGFVVVDESCRTNLPQVWAIGDVVRG
                     PMLAHKAEEEGVAVAERIAGQKPHFDFNTVPWVIYTAPEIAWVGKTEQQLKAERVEYR
                     AGVFPFSANARARALGDTSGLVKVLAASKTDEILGVHIIGPFASELIAEAVVAMEFRG
                     SAEDIGMIIHAHPSLSEALKDAALGVDKRSLNI"
BASE COUNT          657 a         1323 c         1236 g          684 t
ORIGIN      
        1 gagggcagct cgctgaagcg cagggtgcgc gccatctcct tcagcaaggg cggtttgaga
       61 tcggttgcga gacgccgcca gacgagatcg cggatcgggt tggagacgtc gcccgagtta
      121 acgccgagca ggctcacccc gcgcaggatg aaaggcagca cggtggtgtt cagggtgtgg
      181 ctcgccgcca ggccgaccgc ggcgatgacc ccgccatagt tcatggtgct cgccatccag
      241 gcgagcacat cgccgccgag attgtccacc gcccccgccc aagttgcctt gtcgagaggt
      301 ttgattttct gcaggttcag ggtttgccgg agcatgatct ccttcgcgcc gagcttcttc
      361 agccaatccg tctcgctctc cttcccggtc aacgcgacca cgtgatagcc cagcttggcg
      421 agcgccgcta gggcaatcga gccgacgcca ccggttgcac cgttgacgat caccggaccg
      481 tgcgcgggct tgagcccgtt gtgctccatc cggacgatcg cgattcccgc ggtgtaaccg
      541 gcagtgccga acgccatcgc ttcccacagc gacaagccgg gcggcacttt ggcgatccag
      601 tcgcccttta cgcgcgtgta gggtgcgtag cctccgtcgc tcgacacacc gacgtattcg
      661 ccgcttgccg taagaacggc gtcgcccttc ttgaagcgcg gatctctgga ctcgaccacc
      721 gtgccggcga agtcgatgcc gcccacgcac gcgggccttc tcaatatctt ccctttgccc
      781 gtcgccgcaa gcgcatcctt gtagttcacg ctcgagtacg cgatccgcac aagcacttcg
      841 tgcgggtcga gctggtcgag cgtgtagtcg acgaactcgg cgcggatctt cttctccggc
      901 gtttcggtga gcttgtaggc cttgaagcgt tccatggcta ctccggtttt atgccagctt
      961 ccttgaccac gcgggtccac ttttcggttt cctgcgtgac aaaagtcgcg aactcctgcg
     1021 gtttcaggcg cacaacctcg gcgccctcga gctcgaagcg cttgcgcgtc tcgggtgaat
     1081 cgaggatcgc ggctatctcc ttgtagagcc ggtcgagcgc aggctccggc gtgcccgcgg
     1141 gcgcgagaat gccccaccag ttgctcacct cgaacccagg cacgccggcc tcggcgatgg
     1201 tcgggacatc gggcagcgcg gcgacgcgct tcgcgctgct gacaccgagc gccttcagct
     1261 tccccgagcg cacgtgcggg atcatctgca cgaggctgcc gagattcatc tgcacctggc
     1321 cggcgatcac gtcggtgaga gccgggccgc cgcccttgta ctgcacgatc acgaaattgg
     1381 tcttggactg aaggcggaaa agctcggtcg ccatgtgctg gaagctgccg atgccggcgg
     1441 cggccgcatt gagctccccg ggtttctgtt tcaacagatt cagcagatca gtcacggagt
     1501 tcgcggctga ggatgccggc acgacaagca gcgcgggccc gctgccgagc atcgccaccg
     1561 gaacgaagga cttcatcgcg tcccgcggca ggcggttgaa ggcgatgctc gtcggaaagg
     1621 cggtcgaaac gaggagcagc gtgtacccgt cgggcgccgc cttcgccgcc tcgttcgtgc
     1681 cgatgatgct gcccgcgccg ccgcgattgt cgatgaccac attctggccg aggcgctcgc
     1741 cgagctgcat gccgatcgag cggccgacga cgtcattcga gccgcccggc ggaaacggga
     1801 tgatgagacg cacgggcttc acgggatagg actgggcgag cccgagcccg cacgcaaccg
     1861 caagccccag tgcgaccagg gttctcatgc cgccaatgct acccgccttg ccggcagcag
     1921 tcggtattcg gccgactgca tttcatgcag ccggctcacg gtgcgctgga aatccgccga
     1981 gttctggcct tcgacgaaaa gctcctcggg tgcagcccca gccgacacta caagcttgac
     2041 gcgctcgtcg tacagcacgt cgacgagcca ggtgaagcgc cgcgcggcgt ccgactgtct
     2101 cgccgacatg cgcggcacgc cggagagcac gacggtgtgg aagcgcttcg ccaggtccac
     2161 gtaatcggca taggagcgcg gcccgccgca cagcgtgtgg aaatcgaacc agaccacgcc
     2221 gcctgcgcgc ttgcggtagg gaatggtgcg tccgtcgacg tcgagcggat gccattcctc
     2281 ctgcacgtcc ttcaattcct cgaaaatccg cgccagcggc gcatcgctca ggtgatagac
     2341 ctgcacctgc ttcatcttga ggcgccggta gtcggtgccg ctgtccacgg ccaccacgtc
     2401 caggcggctc ttgatcagct cgatcgccgg cacaaagcgc tcgcgctgca ggcccccggg
     2461 atagagctgc tcggggtggt agttcgaagt catgacgaat tgcacgcccc ggtccatgac
     2521 ctgctccagg aagcgtccga ggatcatggc gtcggcgatg tcggaaacgt gaaattcatc
     2581 gaagcacacc aatctatacc gccttgccgt gcgcgccgct acctcggcga tcgggtcctg
     2641 ggcgcctttc agcgcgtcca gctcgcggtg gatctcgcga atgaagtggt ggaaatgcac
     2701 gcgccgcttg cgcacaagcg gcacgcacaa atagaacgca tccatgagga agctcttgcc
     2761 gcgcccgacc gggccccaaa ggtacatgcc cttcggcagc ggcggcttga cgagcaggcg
     2821 cctcagcgcg ctggagcgac gccgcttgta cgcgctccat tcctcgtgca ggcgctgcag
     2881 ccgctcgacc gcgcgccact gcgacgagtc ggagacgaag ccgcgtcgcg cgaggctctg
     2941 ctcgtagaac gcgaccacgc cctgcgcgtc agatattgag gctgcgtttg tcgaccccaa
     3001 gcgcggcgtc cttcagcgcc tcggaaagcg acggatgggc gtggatgatc atgccgatgt
     3061 cctcggccga gccgcggaat tccatcgcca ccaccgcctc ggcgatcagc tccgacgcga
     3121 acgggccgat gatgtgaacg ccgagaatct cgtcggtttt cgacgccgca aggaccttga
     3181 cgaggccgct cgtatcgccg agggcccgcg cacgcgcgtt cgccgagaac gggaatacgc
     3241 ccgcgcggta ctcgacgcgc tccgctttca gctgctgctc ggtcttgccg acccaggcga
     3301 tctcgggcgc ggtgtagatg acccacggca cggtgttgaa gtcgaagtgc ggcttctggc
     3361 cggcgatgcg ctcggctacg gccacgccct cctcctcggc cttgtgcgcg agcatcggtc
     3421 cccgcacgac gtccccgatc gcccacacct gcggcaggtt cgtcctgcag ctctcgtcga
     3481 cgacgacgaa tccgcgctcg tcgagcgtca ggcccacgct gcgcgcattg aggctgtcgg
     3541 aaagcggaat acggccgatg gagacgatca gcttgtcgaa ccgtgccgtc tgtgcggcgc
     3601 gcttggggtc ggtccaggcg acctccacgc catccttgcc ggtggtgaca cccgtgatgt
     3661 tcacgccgag gtggatcgca aggccctgct tcgtgaagac cttctgagcc tccttcgcga
     3721 ccgcctcgtc ggccgcgccg aggaacgccg gcaatgcttc cagcactgtg acctgcgcgc
     3781 ccagacgccg ccagacactg cccatctcga gaccaatcac gccggcgcca accacaccga
     3841 tcgtctcggg aagcgtggga atcgcaagcg cgccggcatt gtcgaggacg ttcacgttgt
//
LOCUS       DASUNT010000196         2662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_113479, whole genome shotgun sequence.
ACCESSION   DASUNT010000196 DASUNT010000000
VERSION     DASUNT010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113479"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..763
                     /locus_tag="VFX94_03805"
     CDS             71..763
                     /locus_tag="VFX94_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEX5767338.1"
                     /translation="MRTALLCLLLVSGCATVEGERDPRDPWEGFNRGVYRFNETFDEY
                     VAQPVARGYVAVLHQEIRTRVSNFFSNIQDLLIGANNFLQGKFEDGVNDWARFAFNST
                     IGLFGIHDVASDFPGLEKHNEDFGQTLGRWGAGPGPYLILPFLGSSTVRDAAGTAVDW
                     AVQPVGEVRPIELRNTLYGLYFVDTRAQLLEASRILEEAALDKYTFQRDAYLQRRRSL
                     IYDGRPPREKIE"
     gene            772..1371
                     /locus_tag="VFX94_03810"
     CDS             772..1371
                     /locus_tag="VFX94_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5767339.1"
                     /translation="MKTALLLLALAALPAAAQDVPPDTLVKNVTLEVVDLIAKDKDIK
                     AGSRAKLVAVIEAKVLPHFNFTAMTALAMGQNWTKASAEQKKELTEEFRTLLVRTYAS
                     ALAAYSEQKLDFRPLRAKATDTDVTVNVRVLQPGAQPVTLDYSMEKTANGWKVYDVVV
                     GGVSLVANYRTEFANTVRASGVDGLIKDLRAKNKSLEKK"
     gene            1368..1652
                     /locus_tag="VFX94_03815"
     CDS             1368..1652
                     /locus_tag="VFX94_03815"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5767340.1"
                     /translation="MIRREGPRITVSGPVTLANATRLLDEGRQHLAEGARTVDLGEVT
                     ELDSALLALILAWLRDARAAKRELSLANVPQSLQTLARLYGVEELLPVAR"
     gene            1649..2503
                     /locus_tag="VFX94_03820"
     CDS             1649..2503
                     /locus_tag="VFX94_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5767341.1"
                     /translation="MKAIEVRSVEKRYGALRALSGITLDIEQGQFFGLLGPNGAGKTT
                     LINVIAGLARPDAGSASVMAADVTSDYRRARRMLGVVPQELVFDPFFTVRETLRIQSG
                     YFGIRRNDAWIDEVMQHLDLTAKADANMRSLSGGMKRRVLVAQALVHKPPVIVLDEPT
                     AGVDVELRQGLWEFVRRLNRDGHTIVLTTHYLEEAEMHCSRIAVLKAGRIVALDSTRN
                     LLHAFAGSRVSARVVARDGSRHLLQIEGYAQLERELGRLRESGVEIADIEIERPDLER
                     VFLRLTSE"
     gene            2500..>2662
                     /locus_tag="VFX94_03825"
     CDS             2500..>2662
                     /locus_tag="VFX94_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX5767342.1"
                     /translation="MSFWTLFTKELLRFVKVSVQTVAAPVLTALLYLVIFGQVLEGRV
                     QVFDGVRYTS"
BASE COUNT          479 a          867 c          876 g          440 t
ORIGIN      
        1 agccagttcc tgttcaacaa ggccgccgag gggaagcccc aggagaagca ggcaaggtga
       61 cgagagtcaa gtgaggacgg cgctgctgtg cctgctgctc gtgtcgggct gcgcgaccgt
      121 ggaaggcgag cgcgacccgc gcgacccgtg ggagggcttc aaccgcgggg tgtaccggtt
      181 caacgagacg ttcgacgagt acgtcgcgca accggtcgcg cgcggctacg tggcggtcct
      241 gcatcaggag atccgcactc gcgtgagcaa ctttttctcc aatatccagg acctcctcat
      301 cggcgcgaac aatttccttc aaggcaagtt cgaagacggg gtcaacgatt gggcgcgctt
      361 cgcgtttaac agcaccattg gtctcttcgg catccacgat gtggcgagcg acttcccggg
      421 gctggaaaag cacaatgagg atttcggtca gacgctcggt cgctggggcg cgggtccggg
      481 gccctacctg atcctgccgt tcctcggttc cagcacggtg cgcgacgcgg ccggcacggc
      541 ggtcgactgg gcggtgcagc cggtcggcga agtgcggccc atcgagttgc gcaacacgct
      601 ctacggcctg tatttcgtcg acacgcgcgc gcagctcctc gaggcgagtc gcatcctcga
      661 ggaagcggcg ctcgacaaat ataccttcca gcgcgatgct tatctgcaac ggcgccgcag
      721 cctgatctac gacggcaggc cgccgcggga gaagatcgaa tgagacctgt catgaagact
      781 gcccttttgc tgcttgcgct cgcggcgcta ccggctgccg ctcaggacgt cccgccggac
      841 acgctggtga agaacgtgac gctcgaagtc gtggacctga tcgccaagga caaggacatt
      901 aaagcaggca gccgcgccaa gctggtcgct gtgatcgagg cgaaagtact gccgcacttc
      961 aacttcaccg cgatgaccgc gctcgccatg gggcagaact ggaccaaggc gagcgccgag
     1021 cagaaaaagg agctcaccga ggaattcagg accctgctcg tacgcaccta cgcgagcgcg
     1081 ctcgcagcgt acagcgagca gaagctcgac ttccggccac tgcgcgccaa ggccaccgac
     1141 accgacgtca cggtgaacgt gcgcgtgctg cagcccggcg cgcaaccggt aacgctcgac
     1201 tacagcatgg agaagacggc gaacggttgg aaggtctacg acgtagtcgt cggcggcgtc
     1261 agcctggtgg cgaactaccg caccgagttc gccaacaccg tgcgcgcctc gggggtcgac
     1321 ggcctgatca aggatctccg ggccaagaac aagtctctgg aaaagaagtg atccggcgcg
     1381 aaggtccgcg aatcaccgtg agcgggccgg tcacgctcgc gaatgccact cgcctgctcg
     1441 atgagggccg gcagcatctc gcagaaggcg cacgcaccgt tgatctcggc gaagtcaccg
     1501 agctcgactc ggcgcttctc gccctgatcc tcgcttggct gcgcgacgca cgtgccgcca
     1561 agcgcgagct cagcttggct aacgttccgc aatcgctgca gacgctcgcc cgtctctacg
     1621 gcgtggaaga gctgctcccg gtcgcccgct gaaagcaatc gaagtccgca gcgtcgagaa
     1681 gcgctatggt gcgctgcgtg cgctgtcggg gattaccctc gacatcgagc aggggcagtt
     1741 cttcggcttg ctcgggccga acggcgcggg caagaccacc ctgatcaacg taatcgcggg
     1801 gctcgcgcgc cccgatgccg gctcggcgtc cgtaatggcg gcggacgtga cgtccgacta
     1861 ccggcgcgcg cgtcgcatgc tgggcgtcgt gccgcaggag ctcgtcttcg acccattctt
     1921 cacggtgcgc gagacgctcc gcatccagtc gggctacttc ggaattcgcc gcaacgacgc
     1981 ctggatcgac gaggtgatgc aacacctcga cctcaccgcc aaggccgacg cgaacatgcg
     2041 ctcgctctcc ggcggcatga agcgccgcgt gctcgtcgcg caggcgctgg tgcacaagcc
     2101 cccggttatc gtgcttgatg agccaaccgc cggcgtcgac gtcgagctac gccaggggct
     2161 ctgggagttc gtgcgacggc tcaaccgcga cggccacacc atcgtcctca ccacgcacta
     2221 cctggaggaa gccgagatgc attgcagccg tatcgcggtg ctcaaggcgg ggcgcattgt
     2281 ggcgctcgac tccacacgca acctgctcca cgcgttcgcg ggttcgcgcg tcagcgcgcg
     2341 ggtggtcgcg cgcgacggca gccggcatct cttgcagatt gagggctatg ctcaacttga
     2401 gcgtgagctc ggccgcctgc gcgagagcgg cgtcgagatc gccgacatcg agatcgagcg
     2461 ccccgacctc gagcgggtgt tcctgcgcct cacctccgaa tgagcttctg gacgctgttc
     2521 accaaggagc tgctgcgctt cgtgaaggtg agcgtgcaga ccgtcgccgc gccggtgctc
     2581 accgcgctgc tgtacctcgt gatcttcggc caggtgctcg agggccgggt gcaggtattc
     2641 gacggggtgc gttacacgtc gt
//
LOCUS       DASUNT010000197         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1253758, whole genome shotgun sequence.
ACCESSION   DASUNT010000197 DASUNT010000000
VERSION     DASUNT010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253758"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFX94_03830"
     CDS             <1..258
                     /locus_tag="VFX94_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5767343.1"
                     /translation="DGGAAIEVCEAFRPHVAVLDIGMPVRNGYDVARHLRARRGRDLT
                     LIALTGWGGDGDVQRAREAGFDAHLTKPVDPGALNEMICRA"
     gene            complement(233..1249)
                     /locus_tag="VFX94_03835"
     CDS             complement(233..1249)
                     /locus_tag="VFX94_03835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5767344.1"
                     /translation="MHYGIFDWVEASRRAPAEVYEHKLELAAAADRAGFDGFFVAEHH
                     GTPLSIDGSPTALLSAIFQRTRHLRAGALTFCLPWYHPYRFYSEVCMLDHLSYGRLEL
                     GVGRGVSPIESRIFGLQSIEESRERYRATLEMFFTACTSSTMKVGGVDAELHVTPYQK
                     PYPPLWFPSSNKESIEFTARHGYNTAFIGKHADCRPMFERYRELWEKHRADPGRHNAH
                     VAAPFLARTQHVVIAETDAEAEKLGLQAYETWAGHMHHLTRRLGRPDVHKTTPFDEDS
                     AQRLIAGSPLTALEELDEMVRLTGANYLLCIFSFGDIAPDHAIRSLELFAEEVMPRLG
                     RSSR"
     gene            complement(1250..>2498)
                     /locus_tag="VFX94_03840"
     CDS             complement(1250..>2498)
                     /locus_tag="VFX94_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5767345.1"
                     /translation="WPVSLADVEAIQRDRKRVAVERALQSPYLKKRIPKGRIEDIWQR
                     IPVLTKEELRSIAPERFHEEFCIQPRAKAVEYWRSGGATGRPLFYPRSAEDMQHGLLA
                     FERAWALIGASAEDCAHISFPLGVHPVAHLYARAAINRGIGTVWCGAGTNTPSEVQLE
                     LIDHLKPTIWLGMASYGLHLANLAETKGFDLKNSTVKKIIVAAEPLSPVKRKKLEDAW
                     GAEVFDHFGMTEAAFVAGEGVGHQGLHVWADQFFVEVLDERTGDPVGDGEVGSLVVTP
                     LWNNTMTPFLRWSSGDLASVHKAKPPGKYPWSVFPTLRHARRTVGFFKVRGVNINHAE
                     LEDALFGDPDAVDFRAEVVNEGDNDVLRVFVETRSGEATRIRDCIRNMFHITPEVTLL
                     ARGTIAREFEAAVKPPRFVDKRG"
BASE COUNT          394 a          818 c          877 g          409 t
ORIGIN      
        1 gacggcggcg cggcgatcga ggtgtgcgag gctttccgcc cgcatgtcgc ggtgctcgac
       61 atcggcatgc cggtgcgcaa cggctacgac gtcgcgaggc atctgcgcgc gcgccgcggc
      121 cgggacttga cgctgatcgc gctcaccggc tggggcggcg acggcgacgt gcagcgcgcc
      181 cgcgaagcgg gcttcgacgc tcacctcacc aagccggtgg atccgggggc gctcaacgag
      241 atgatctgcc gagcctaggc atcacttcct cggcgaagag ctcgagcgag cggatggcgt
      301 ggtccggggc gatgtccccg aacgagaaga tgcacagcag gtaattcgcc ccggtaaggc
      361 ggaccatctc gtcgagctct tcgagcgccg tgagcgggga gcccgcgatc aggcgctgcg
      421 cggagtcctc gtcgaagggc gtggtcttgt gcacgtccgg ccggccgagc ctgcgcgtga
      481 gatggtgcat gtgcccggcc cacgtctcat aggcctgcag gccgagcttc tcggcctcgg
      541 catcggtctc ggcgatcacc acgtgttgag tgcgcgcgag gaaaggggcg gcgacatgcg
      601 cgttgtgccg gcccggatcg gcgcggtgct tctcccagag ctcgcggtag cgctcgaaca
      661 tcggcctgca gtcggcatgc ttgccgatga acgcagtgtt gtagccgtgg cgcgccgtga
      721 actcgatcga ctccttgttc gagctcggaa accagagcgg cgggtagggc ttctggtaag
      781 gcgtgacatg cagttcggcg tccaccccgc ccactttcat cgtggaagac gtgcacgccg
      841 tgaagaacat ttcgagcgtg gcgcgatagc gctcgcgcga ctcttctatg ctttgcaggc
      901 caaagatcct ggattcgatc ggcgaaaccc cgcgtccgac tcccagctcg aggcgcccgt
      961 acgacaggtg gtcgagcatg cacacctcgc tgtagaagcg gtaggggtgg taccagggca
     1021 ggcagaacgt aagagcgccc gcgcgcaggt ggcgcgtgcg ctggaagatg gcggaaagga
     1081 gcgcggtcgg cgagccgtcg atcgagagcg gcgtgccgtg atgctcggcg acgaagaagc
     1141 cgtcaaaccc ggcgcggtcg gccgccgcgg cgagctcgag cttgtgctcg tacacctcgg
     1201 ccggcgcgcg gcgcgaggct tcgacccagt cgaaaattcc gtagtgcatt cagccccgct
     1261 tgtcgacgaa gcgcggcggc ttcaccgccg cctcgaactc tcgcgcgatc gtgccgcgcg
     1321 cgaggagcgt cacttcgggg gttatgtgga acatgttccg gatgcaatcg cggatacgcg
     1381 tcgcctcgcc ggatcgcgtt tcgacgaaca cgcgcagcac gtcgttatcg ccctcgttca
     1441 ccacctcggc gcggaaatcg accgcatccg ggtcgccgaa aagggcgtcc tcgagctcgg
     1501 cgtggttgat gttgacgcca cgcaccttga agaagccgac cgtgcggcgc gcgtgcctca
     1561 aagtcgggaa caccgaccag gggtacttgc cgggcggctt cgccttatgc acgctcgcca
     1621 ggtcgcccga gctccagcgc aggaacggcg tcatggtgtt gttccagagc ggcgtgacga
     1681 cgagcgagcc cacctcgccg tcgcccaccg ggtcgccggt gcgctcgtcg aggacctcga
     1741 cgaagaactg gtcggcccag acgtgcagcc cctggtggcc gacgccctcg ccggcgacga
     1801 acgcggcctc ggtcatgccg aagtggtcga acacctcggc gccccaggca tcctcgagct
     1861 tcttgcgctt cacgggggag agcggctcgg cggccacgat gatctttttt accgtggaat
     1921 tcttgagatc gaagcccttc gtttccgcga ggttcgccag gtgcagtcca tagctcgcca
     1981 tgccgagcca gatcgtgggc ttgaggtggt cgatgagctc cagctgtacc tcggagggcg
     2041 tgttcgtccc ggcgccgcac cacacggtgc cgatgccgcg gttgatcgcc gcgcgcgcgt
     2101 agagatgcgc caccgggtgc acgccgagcg ggaaggagat gtgcgcgcag tcctcggcgc
     2161 tcgcgccgat cagcgcccag gcgcgctcga acgcgagcag gccgtgctgc atgtcctcgg
     2221 ccgagcgcgg gtagaagagc ggccggccgg tggcgccgcc cgagcgccag tactcgaccg
     2281 ccttcgcgcg cggctggatg cagaactcct cgtggaaccg ctccggcgcg atggagcgca
     2341 gctcctcctt ggtgagcacg gggatgcgct gccagatgtc ctcgatgcgc cctttgggga
     2401 tgcgtttctt aaggtacggc gactgcaacg cgcgctcgac cgccacgcgc ttcctgtcgc
     2461 gctggatggc ctcgacatcc gcgagcgaca ccggccag
//
LOCUS       DASUNT010000198         3412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_989704, whole genome shotgun sequence.
ACCESSION   DASUNT010000198 DASUNT010000000
VERSION     DASUNT010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989704"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..757
                     /locus_tag="VFX94_03845"
     CDS             122..757
                     /locus_tag="VFX94_03845"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX5767346.1"
                     /translation="MLLGIVPFGIITGVAMVASGIPPLVAMAMSLLVFAGASMVASAQ
                     LLGSGAPAVLIVFTTLVINLRFMMYSASLRLHFSRAPLPQRLAIAYLSADNVYGLLLG
                     RFSEHPDDPGKLEYFLGAGLVVWAVWQAAVLAGVMIGAGVPSAWRLEFAAPLAFIAMS
                     VPLLRDRAMVAAAVAAATIIACSHAIPLRLNVILAAAAGIAAGLVAEKWKR"
     gene            745..1041
                     /locus_tag="VFX94_03850"
     CDS             745..1041
                     /locus_tag="VFX94_03850"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX5767347.1"
                     /translation="METLTLWLLLVAMALVTFALRASFLLLPPGVELPPLFRRALRYV
                     PAAVLTAIWAPEVLVKSEQLPAGVVALAVAWRWRMTFATIIAGLAALHLFAWLT"
     gene            1029..>3412
                     /locus_tag="VFX94_03855"
     CDS             1029..>3412
                     /locus_tag="VFX94_03855"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX5767348.1"
                     /translation="MAHLRKAFAVFLLLCLTSALAAAAPQKTVSIEGITEWHLANGLK
                     LLTVPDPSADTVTVHITYLVGSRHEGYGEKGMAHLLEHLLFKGSKRHPNVKEEFTRRG
                     ARWNGTTSNDRTNYFQTLAATDDNLEWAIAMEADRMVNSFVSKQDLDSEMTVVRNEFE
                     MGENNPGSVLFQRMQQLAFPWHNYGNAIIGSRADIENVPIDKLQAFYRTWYQPDNAVL
                     ILAGRFDEAKALGLVQKHFGAIARPKRVLPAFYTSEPTQDGERRVRLERVGDNQLVSV
                     LYRVPAGGHPDYPALDVLVNILGDVPAGRLHRAIVQKGLASQSWGAERALHDPGYVFF
                     GASLGREQKLADARERLLEVVEGAGREKVLPDELERARTTLLNEFEKVQLDTNTLVRS
                     LTEFIAIGDWRLFFLYRDRLRKVTLEDVQRVAAQYLKPANRVLGEFVPTERPERAEIP
                     PTPDLQSVLAGYTGGAAVRLGEAFDPSPQNIDKRTIKRQVSNGIGVALLPKQTRGGRV
                     VASLTLHWGDEKSLMHRETACNFAGAMLMRGTQKRSRAEFKDALERLNASVQLNGEGA
                     YIEVRGENLVPALELVAEALREPAFSAAEFDEMKRAALTSAEGQRQEPASLASVRLAR
                     HLHAYAPGHPHYTPTVEERIELVRKTTLEDAQACYRELYGASGADFVAVGDFDPEAVA
                     QAVDRLLGPWRTPHPFQRVPSLYFDRPGFEDALVTPDKANAALRAGINVRMRDDHPDF
                     PALVLANHLLGGSSTARVPARVREKEGLSYSTYTTFSSSALDEAASFRVGSIFAP"
BASE COUNT          526 a         1219 c         1104 g          563 t
ORIGIN      
        1 cccgggcacg ccatccggaa cgcgacgctg aagcgcggcg aggcgtagcc cgctcaccgg
       61 gaacgtcagg ctgttctacc ggcatcccgc ttttgccgcg ggggtgcgcg catcgctgcc
      121 cgtgctcctc ggcattgtcc ccttcggcat catcaccggc gttgccatgg tggcgagcgg
      181 catcccgccg ctcgtcgcga tggcgatgtc gctcctcgtg ttcgccggtg cctccatggt
      241 ggcctcggcg cagctcctcg gctccggcgc gcctgccgtc cttatcgtct ttactacgct
      301 cgtcatcaat ctgcgcttca tgatgtacag cgcgtcgctg cggctgcact tctcgcgtgc
      361 gccgctgccg cagcgccttg ccatcgccta cctcagcgcc gataacgtct atggactgct
      421 cctcgggcgc ttttccgagc atcccgacga tcccggcaag ctcgagtact tcctcggcgc
      481 cgggctcgtg gtgtgggcgg tgtggcaggc ggcggtgctc gccggggtga tgatcggcgc
      541 cggcgtgccg agcgcctggc ggctcgagtt cgcggcgccg cttgcgttca tcgcgatgtc
      601 ggttcccctc cttcgcgacc gcgccatggt ggcggcggcg gttgccgccg ccacgatcat
      661 tgcgtgttcg cacgctatcc ccctgaggct gaacgtgatc cttgccgcgg ccgccgggat
      721 cgcagcgggc ctggtcgccg aaaaatggaa acgctgacgc tctggctcct cctcgtcgcg
      781 atggcgctcg tcacgttcgc gctgcgcgcc tcgttcctgc tccttccgcc gggcgtggag
      841 ctgccgccgc tctttcgccg cgcgctgcgc tacgtgccgg cggcggtgct gaccgcgatc
      901 tgggcgccgg aagtgctcgt gaaaagtgaa caattgcccg ctggagttgt cgcattagcg
      961 gtggcgtggc gctggcgcat gaccttcgct acgataatcg cggggctcgc cgcacttcac
     1021 ttattcgcat ggctcaccta agaaaagctt tcgccgtatt cctgctgctg tgcctgacca
     1081 gcgctctcgc cgccgcggcg ccgcagaaaa ccgtttccat cgaaggcatc accgagtggc
     1141 atctcgccaa cgggctgaag ctcctcaccg tgcccgatcc gagcgcggac acggtcaccg
     1201 tgcacatcac ctacctcgtc ggctcgcgcc acgaggggta cggcgagaaa ggcatggcgc
     1261 atctcctcga gcacctcctc ttcaagggct ccaagcgcca tccgaacgtc aaggaggagt
     1321 tcacgcgccg cggcgcgcgc tggaacggca cgacctcgaa cgaccgcacc aactatttcc
     1381 agaccctcgc ggcgaccgac gacaatctgg agtgggcgat cgccatggag gccgaccgca
     1441 tggtgaactc gttcgtctcc aagcaggacc tggacagcga gatgaccgtg gtgcgcaacg
     1501 agttcgagat gggcgagaac aaccccggca gcgtactctt ccagcgcatg cagcagcttg
     1561 ctttcccgtg gcacaactac ggcaacgcga tcatcggcag ccgcgccgac atcgagaacg
     1621 tgcccatcga caagctgcag gcgttctatc gcacctggta tcagcccgac aacgcggtgc
     1681 tgatcctcgc cggacgcttc gacgaagcga aggcgctcgg cctggtgcag aagcacttcg
     1741 gcgccatcgc ccgccccaag cgcgtgcttc ccgcgttcta caccagcgag ccgacgcagg
     1801 atggagagcg ccgcgtgcgc ctcgagcgtg tcggcgacaa ccagctcgtg tcggtgctct
     1861 accgcgtacc cgccggaggc catcccgact acccggcgct cgacgtgctc gtgaacatcc
     1921 tcggcgacgt cccggccggc cgcctgcacc gcgcgatcgt gcagaaaggg ctcgcctcgc
     1981 agtcctgggg tgcggagcgc gcgctgcacg acccgggtta cgtgttcttc ggcgcttcgc
     2041 tcggcaggga gcagaagctc gccgatgcgc gcgagcggct gctggaggtc gtcgaaggag
     2101 cggggcgcga aaaggtcctt ccggatgagc tcgagcgcgc ccgcaccacg ctgctcaacg
     2161 aattcgaaaa ggtgcagctg gacaccaaca cgctcgttcg ctcgctcacc gaattcatcg
     2221 ccatcggcga ctggcggctt ttcttcctgt accgggaccg cctgcgcaag gtgacgctcg
     2281 aggacgtgca gcgcgtcgcc gcgcagtacc tgaagccggc caaccgggtg ctcggcgaat
     2341 tcgtgcctac cgagcgtccc gagcgcgccg agatcccgcc gacgccggac ctgcagtcgg
     2401 tgctcgcggg ctacaccggc ggcgcggccg tgcggctcgg cgaggccttc gatccctcgc
     2461 cgcagaacat cgacaagcgc acgataaagc gccaggtctc caacgggatc ggcgtggcgc
     2521 tgctcccgaa gcagacgcgc ggcgggcgcg tcgtcgcttc cctcaccctg cactggggag
     2581 acgaaaaaag tctgatgcac cgcgagaccg cgtgcaactt cgccggcgcc atgctgatgc
     2641 gcggcacgca aaagcgcagc cgcgccgagt tcaaagacgc gctcgagcgc ctgaacgcat
     2701 cggtgcagct gaacggcgaa ggcgcctaca tcgaggtacg cggcgaaaac ctcgtgccgg
     2761 cgctcgagct cgtcgccgaa gcgctgcgcg agcccgcgtt ctcggcggcc gagttcgacg
     2821 agatgaagcg cgccgcgctc accagcgccg aggggcagcg ccaggagccg gcatcgctcg
     2881 ccagcgtgcg gctggcgcgc cacctgcatg cgtacgcgcc ggggcatccg cattacacgc
     2941 cgaccgtcga ggagcgcatc gagctcgtgc gcaagaccac cctcgaggac gcgcaagcct
     3001 gctaccgcga gctctatggg gcgagcggcg cggatttcgt cgcggtgggc gacttcgatc
     3061 ccgaggcggt ggcgcaggcg gtggatcgcc tgctcggccc ctggcgcacg ccgcacccct
     3121 tccagcgcgt gccgtcgctt tacttcgacc gccccggctt cgaagacgcg ctcgtcaccc
     3181 cggacaaggc gaacgcggcg cttcgtgccg gcatcaacgt gcgcatgcgc gacgaccatc
     3241 ccgatttccc ggccctcgtg ctcgccaacc acctgctcgg ggggagctct acggcgcgcg
     3301 tgccggcgcg agtgcgcgag aaggaaggtc tctcctacag cacctacacc accttcagct
     3361 cgagcgcgct cgacgaagcg gcgtcgttcc gcgtcggctc catctttgcg cc
//
LOCUS       DASUNT010000199         2565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_814999, whole genome shotgun sequence.
ACCESSION   DASUNT010000199 DASUNT010000000
VERSION     DASUNT010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814999"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VFX94_03860"
     CDS             <1..789
                     /locus_tag="VFX94_03860"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5767349.1"
                     /translation="QSVVVENVVGAGGNVAAARVAKAPPDGYTVLAHSSAYAVNPTLI
                     PNAGYDGEKDFIALAVVASQPNLIVVHADFPAKTLAELLARARTEKLAFASAGNGTTP
                     HLTGENLFKIRAKVDITHIPFKGGGPAAAAVLSGQPPIGSIAGSAPLPHIKAGKLRAL
                     AVSSAQRLAALPEVPTLGELGFPGMEDYTWVGFFAPAGTPSEVAQKLNDALLNAARDP
                     QIKERLEGLAFEVTAAPLADTAAYVKSELAKWGKVVRDVGAKID"
     gene            complement(870..2132)
                     /gene="egtB"
                     /locus_tag="VFX94_03865"
     CDS             complement(870..2132)
                     /gene="egtB"
                     /locus_tag="VFX94_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEX5767350.1"
                     /translation="MQPYSPKQPRPRRGQLEDYRRIRETTLALIAPLSPEDCSIQSMP
                     DASPAKWHLAHTTWFFETFVLQPHIPGYRVFDPAYAYLFNSYYNALGERHPRPERGML
                     SRPSLDDVLAYRRHVDELFFEVENLSPEALALIELGLHHEQQHQELILTDVKHLLSRN
                     PLKPAYQKTWPLTPIRASEARWIEIPGGLYEIGHGGPGFAFDNEKPRHRVWLEDFEVA
                     SHPVTHGDFLAFIADSGYRRADLWLSAGWDIVRSRGWQAPEYWTQRDGAWHTFTLHGE
                     VPVDPNTPICHVSFYEAEAFARWANARLPTEAEWEVAARGLPVEGNFMESGALHALAL
                     RGDVEDGALAQAFGDVWEWTRSDYAPYPGFKSAAGAVGEYNGKFMSGQYVLRGGSCAT
                     PQSHIRLTYRNFFPPDARWQFSGLRLAR"
     gene            complement(2182..>2565)
                     /locus_tag="VFX94_03870"
     CDS             complement(2182..>2565)
                     /locus_tag="VFX94_03870"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX5767351.1"
                     /translation="ELVLTARAENFVRGRPDLDDTIKRLQAFEKAGADVLMAPGLPDL
                     ESVRKVCGALSKPFNFMAGIKGKSFSVAELQSAGVRRISLATSLWRAAMSELIEAARE
                     VKEKGTLGYVERSLPTAEIVKLMER"
BASE COUNT          445 a          866 c          824 g          430 t
ORIGIN      
        1 cagagcgtcg tcgtggagaa cgtcgtcggc gccggcggca atgtcgccgc ggcgcgagtc
       61 gccaaggcgc cgcccgacgg ctacaccgtg ctcgcgcatt cgtcggccta tgcggtgaac
      121 ccgaccctca ttccgaacgc cggatacgac ggcgaaaagg acttcattgc gctcgccgtc
      181 gtcgcctcgc agccgaacct cattgtcgtg cacgccgact ttccggcgaa gacgctagcc
      241 gagctgcttg cgcgcgcccg caccgagaag cttgccttcg cttcggccgg taacggcacc
      301 acgccacacc tcaccggcga gaacctgttc aagatccgcg ccaaggtcga catcactcac
      361 attcctttca aaggaggcgg gccggcggcc gcggccgtgc tgagcggcca gccgccgatc
      421 gggtcgatcg ccggctccgc gcccctgccg catatcaagg ccggcaagct gcgcgcgctc
      481 gcggtatctt cggcgcagcg ccttgcggcg ctgcccgaag tgccgaccct cggcgagctg
      541 ggcttcccgg gaatggagga ctacacctgg gttggctttt tcgcacccgc cggcacgccg
      601 tccgaggtcg cccagaagct caacgatgcg ttgctgaacg ccgcgcgcga cccccaaatc
      661 aaggagcggc tggaggggct cgcgttcgaa gtgaccgcgg cccccctcgc cgacactgcc
      721 gcctacgtca agagcgagct cgcgaagtgg ggcaaggtgg tacgcgacgt cggcgcaaaa
      781 atcgactagc tcgaaggaac gtccggggaa tagactaggt gggaaagcag tcgcccccgc
      841 gaaagcgggg gcccgtcttt tcgtgtcact taccgggcaa gcctcagccc cgagaactgc
      901 cagcgcgcat cgggtgggaa gaaattccgg taggtcaggc ggatatggct ctgcggggta
      961 gcgcacgagc cgccgcgcag cacatactgc ccggacatga atttgccgtt gtattcgccg
     1021 accgcgccgg ccgcggactt gaaccccggg tacggggcat agtcgctgcg cgtccattcc
     1081 cacacgtcgc cgaacgcctg cgccagcgcg ccgtcttcca catcgccacg cagggcgagc
     1141 gcatggagcg cgccgctctc catgaagttg ccttcgaccg gcaagccgcg agcggctacc
     1201 tcccattccg cttcggtggg aaggcgcgcg tttgcccagc gcgcgaacgc ttccgcctcg
     1261 tagaagctca catggcagat cggcgtgttg gggtccaccg gaacctcgcc gtgcagcgta
     1321 aaggtatgcc aggcgccgtc gcgctgcgtc cagtactcgg gcgcttgcca gccgcgagag
     1381 cgcacgatgt cccagcccgc cgacagccat aggtcggcac gccggtagcc gctgtcggca
     1441 atgaaagcga ggaagtcgcc gtgggtgacg ggatgcgaag ccacctcgaa atcctcgagc
     1501 cacacccggt gacgcggctt ctcgttgtcg aacgcgaagc ccggcccgcc atgcccgatc
     1561 tcgtagaggc cgcccggaat ctcgatccag cgcgcctcac tcgcgcggat cggcgttagc
     1621 ggccaggtct tctggtaggc gggcttcagc ggattgcgcg acagcaggtg cttcacgtcg
     1681 gtcaggatca gctcctggtg ctgctgctcg tggtgaagcc ccagctcgat cagcgccagc
     1741 gcctcgggcg aaaggttctc gacttcaaag aacagctcat ccacgtgccg ccggtatgcg
     1801 agaacgtcgt cgagcgacgg ccgcgatagc atgccgcgct ccgggcgcgg gtggcgctcg
     1861 ccgagcgcgt tgtagtacga attgaacagg taggcgtaag ccggatcgaa cacccggtag
     1921 cccgggatgt gcggctgcag cacgaacgtc tcgaaaaacc aggttgtatg cgcgagatgc
     1981 cacttcgccg ggctcgcatc gggcatcgac tgaatggaac agtcctccgg cgaaagaggc
     2041 gcgatgagcg cgagcgtcgt ttcgcggatc ctgcggtagt cctcgagctg accccggcgg
     2101 ggcctcggct gcttcggaga ataaggctgc acgacacctc cagtacgctc tggaccgtgt
     2161 tattcgtcca tcgttctcct tttatctctc catcagtttc actatttcag ccgtgggtag
     2221 cgagcgctcc acgtagccca aggtgccctt ttccttcact tcacgcgcag cttcgatcag
     2281 ttcgctcatg gccgcgcgcc agagcgaggt ggcgaggctg atgcggcgca caccggccga
     2341 ctgcagctcc gcgacgctga aggacttgcc tttgatgcca gccatgaaat tgaacggctt
     2401 cgagagcgcg ccgcaaacct tgcgcacgga ttccaggtcg ggaagcccgg gcgccatgag
     2461 cacgtccgcg cccgcttttt cgaacgcctg tagccgcttg atggtgtcgt cgaggtcggg
     2521 cctgccccgc acgaaatttt ccgcacgcgc cgtcaggacg agctc
//
LOCUS       DASUNT010000200         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_113914, whole genome shotgun sequence.
ACCESSION   DASUNT010000200 DASUNT010000000
VERSION     DASUNT010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113914"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VFX94_03875"
     CDS             <1..555
                     /locus_tag="VFX94_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5767352.1"
                     /translation="VCLSKLPLGWNGAYRHFRHPLDYLGADGGGGVGAGPGLTVGAAL
                     ALKGSERMVVGIMGDGDYLMGVTALWTATHYKLPCLMLVANNRSFYNDEMHQERVARE
                     RGRPVENKWIGQRIDEPDIDLAAMARAQGAVGIGPVKQLHELKPSLQRAIEHVKHGQV
                     CVVDVRVVAGYDAPMSGAAMPTRR"
     gene            556..1761
                     /locus_tag="VFX94_03880"
     CDS             556..1761
                     /locus_tag="VFX94_03880"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5767353.1"
                     /translation="MASPLAHREFRTLWSGSLLSWWSQWIQHAALGWVVYEITGSGAL
                     LGAVMGVRAIPMVLLTPVSGMAADRYDRRRLMQASQGLAAAVSLAFGAALALGIVGTW
                     MLFAFTMLMGASNVMDRPARFTTAFELVPRELAVQAVALNTIGFSMARVLGPVMAGYI
                     IAAVGGAGSFFVQGVLYAASGAMVLMVAFPARRERKRERSALGEMAEGLRFAVSNPRT
                     RVLLMVGLLPFFLLIPVFGTLYPIYAKDEFAAGPTGLGLLLTAVGVGGTLGGFIGNAL
                     GRVERQGLLQAIWVVVIGASIIGLALSPTLALAMAFSVIGGAAEMAHASSNMAMMQIS
                     APEEMRGRISALLMLNPAFISLGALVAGPLSDAVGVRSASIILATTAIAAVLVLYARS
                     STLRELRHK"
     gene            1758..>2462
                     /locus_tag="VFX94_03885"
     CDS             1758..>2462
                     /locus_tag="VFX94_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5767354.1"
                     /translation="MKKPKLSASKALAEDLVAASRILAQHGVLDAYGHVSARSDRNPE
                     RFMMSRSLAPALVTAADLMELDENSEPLPGDKRKGFLERYIHGEIYRARPDVMAVVHS
                     HSPSVIPFGVTRTKLRPVYHMGSFLWSGAPVFDIRIVREENDLLIRDNTLGKALATTL
                     SFHTCVLMRGHGMTVIGDGVREAVFRAIYTEMNARLQLQATQLEGPIEFLSDEEGRRS
                     TVSNRGTVERPWELWKR"
BASE COUNT          375 a          842 c          819 g          426 t
ORIGIN      
        1 gtttgcctct cgaagctccc gctcggctgg aacggcgcat accggcattt ccgccatccg
       61 ctcgactacc tgggcgcgga cggcggagga ggcgtaggcg ccgggccggg gcttacggtt
      121 ggcgcggcgc tcgcgctcaa aggctcggag cgcatggtcg tcgggatcat gggcgacggt
      181 gactacctta tgggcgtcac cgccctctgg accgcgaccc actacaagct gccctgcctg
      241 atgctcgtcg ccaacaaccg ctccttctac aacgacgaga tgcatcagga gcgcgtcgcg
      301 cgcgagcgcg gccgcccggt ggagaacaag tggatcgggc agaggatcga cgagcccgac
      361 atcgaccttg ccgccatggc gcgtgctcag ggtgcggtag gcatcggacc cgtcaagcaa
      421 ctgcatgaac tgaagccttc cctgcaaaga gcgatcgagc atgtgaagca cggccaagtc
      481 tgtgtggtcg acgtgcgcgt ggttgcgggc tacgacgcgc ctatgagcgg agccgcaatg
      541 ccgacgaggc gctgactggc gtcaccccta gcccaccgcg agttccgcac cctctggtcg
      601 ggctcgctgc tctcgtggtg gagccagtgg atccagcacg ccgcgctcgg ctgggtggtg
      661 tacgagatca ccggctcggg agcgctcctc ggcgcggtga tgggcgtgcg cgcaattccc
      721 atggtgctcc tcacgccggt gtccggcatg gcggccgacc gctacgaccg gcgccggctc
      781 atgcaggcga gccaggggct cgcggccgcg gtgtcgctcg ccttcggcgc cgcgctcgca
      841 ctcggcatcg tgggcacctg gatgctcttc gccttcacga tgctgatggg cgcctcgaac
      901 gtcatggacc gcccggcgcg cttcactacc gctttcgagc tcgtgccgcg cgagctcgca
      961 gtgcaggcgg tcgcgctgaa cacgatcggc ttctccatgg cgcgcgtgct cggcccggtg
     1021 atggcgggct acatcatcgc cgcggtgggc ggagcgggaa gctttttcgt ccagggcgtg
     1081 ctctatgccg cttcaggcgc aatggtgctg atggtggcgt tcccggcgcg tcgcgagcgc
     1141 aagcgggagc gctcggcctt gggcgagatg gcagaaggac tgcgcttcgc ggtctcgaac
     1201 ccgcgcacgc gcgtgctgct catggtggga ttgctgccct tctttctcct catcccggtg
     1261 ttcggaacgc tctatccgat ctacgccaag gacgagttcg ccgccgggcc gaccggcctg
     1321 gggctcctcc tcaccgcggt cggcgtcggc ggcacgctcg gcggcttcat cggcaacgcg
     1381 ctcggccggg tggagcgcca ggggctgctg caggcaatct gggtcgtggt gatcggcgcc
     1441 tccatcatcg gcttggcgct cagcccaacg ctcgcgctcg ccatggcgtt cagcgtgatc
     1501 ggcggcgcgg ccgagatggc gcacgcctcg agcaacatgg cgatgatgca gatctcggcg
     1561 cccgaggaaa tgcgcggccg catctcggcg ctgctcatgc tcaaccccgc gttcatctcg
     1621 ctcggcgcgc tcgtcgccgg cccgctctcc gacgcagtcg gcgtgcgcag cgcctccatc
     1681 attcttgcaa ccacggccat cgccgcggtg ctcgtcctgt atgcccgttc ttccacgtta
     1741 cgggagttgc gccacaaatg aagaagccga agctttccgc cagcaaagcg ctcgccgagg
     1801 atcttgtcgc cgcaagccgg atcctcgcgc agcacggcgt gctcgacgcc tacggccacg
     1861 tcagcgcgcg cagcgaccgc aaccccgaac gcttcatgat gtcccgctcg cttgctccgg
     1921 cgctcgtcac ggcggcggac ctgatggagc ttgacgagaa cagcgagccg ctacccggcg
     1981 acaagcgcaa gggattcctc gagcgctata tccacggcga gatttaccgc gcccggccgg
     2041 acgtgatggc cgtggtgcac agccactccc cttcggtcat cccgttcggc gtcacgcgca
     2101 cgaagctgcg cccggtctac cacatgggat cgttcctctg gtccggcgcg ccggtgttcg
     2161 atatccggat agtgcgggaa gaaaacgacc tcttgatccg cgacaacacg ctcggcaagg
     2221 cgctggcaac gactctttcc tttcacactt gcgtgctaat gcgcggccac ggcatgaccg
     2281 tgatcggcga cggtgtccgc gaggcggttt tccgcgccat ctacaccgag atgaacgcgc
     2341 gcctgcaact ccaggccact cagctggaag gcccgatcga gttcctgagc gacgaggaag
     2401 gcaggcgctc caccgtgtcc aaccgcggca cggtggagcg cccttgggaa ctttggaaga
     2461 ga
//
LOCUS       DASUNT010000201         5363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_990265, whole genome shotgun sequence.
ACCESSION   DASUNT010000201 DASUNT010000000
VERSION     DASUNT010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990265"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFX94_03890"
     CDS             complement(<1..478)
                     /locus_tag="VFX94_03890"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5767355.1"
                     /translation="MKRFAIIALVAALLPFEAAAQSYPSKPVRMVNPFPPGGPLDVLG
                     RILSERLGASLGRPVLVENRPGAAGNIGAAEVARSPADGHTLLLTLFSIVTSNPHLYE
                     RLPFDPLKDFAPITALASSDSVLIVHPSLPAASAQALIAHAKANPGKVNYASAGKAS"
     gene            complement(483..932)
                     /locus_tag="VFX94_03895"
     CDS             complement(483..932)
                     /locus_tag="VFX94_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767356.1"
                     /translation="MKVFVLTLILSVPGIVLAASDPVTPETRDPVLDKATEATKRQDW
                     TGAAAVLKDAVAANPNNANYHNLYAYSIRKGASPDMNLVFKHYNEALRIDPNHRGAHE
                     YIGEAYLMVGNVAKAKEHLGALDKLCFFPCAEYTDLKKAIAAYEAKR"
     gene            955..1596
                     /locus_tag="VFX94_03900"
     CDS             955..1596
                     /locus_tag="VFX94_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5767357.1"
                     /translation="MVLLAVALTAGAPAAFPQPPQTRAEALGALQSPDAARRAEAVVW
                     IANRGGMDDAPLLHERLRDESAFVRGFAEQGLWLLWSRSGDAAVDRLMALGIEAMQAG
                     RHSEAIGLFSEIIETRPDFAEGWNKRATVYYLAGQYGKSIADCHEVLKRNPGHFGALS
                     GMGQIYLQLEDDEQALQWYRRALEVNPNMLGVEMSLRMLEERLRTRRAPGRST"
     gene            complement(1541..1837)
                     /locus_tag="VFX94_03905"
     CDS             complement(1541..1837)
                     /locus_tag="VFX94_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767358.1"
                     /translation="MSRNGGLGLKIDPRIEYEIYRTIPHDMMRHLAQLEVPAAFIGGA
                     DSDVVRRIRLAGMRPKFSFRKVAGGHLFPFEHPREAARSIAQALAGFEAAPRAS"
     gene            complement(2335..>5363)
                     /locus_tag="VFX94_03910"
     CDS             complement(2335..>5363)
                     /locus_tag="VFX94_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5767359.1"
                     /translation="ATPILSAGRQLDAEWEMRRKDGSTFLCRVVAKAIDPANTHQGTV
                     WIVEDITERRRHADEMARVLREHEALLSTASVGIAFVKDRRFVRANRRYEEMYGYGPG
                     ELHGQSVGAIYPRAEDFARAEGIYDALARGQTMRRIELRRRKDGSTFWNRADGRALDP
                     QNPHKGSVWTVEDVTEQRRAEEELQRVLAEQQALLDNVVVGIAISRSRKLVRCNRRFE
                     EMFGFADGQAIGVSWREMYFTDEEFELRSQVIAELDQGRMHTREQWLRRQDGSGFWCR
                     ISARAVAPGDPSKGHVWLIEDITERRRADEALERLVREQDAVLQNAVTGIIFVKDRRI
                     VRCNRRFEEIFGCGAGELINSSTRFMFATDREYELAGESLYEPVWRGETVYVERRHVR
                     KDGTLIWCSISGRAVQPGDPSQGSVWLFEDITQEHDSEERVQRALAEQELILDNASVG
                     IAFVRSRAIQRCNRFLEDMVGAGPGELIGQSSAVLFDSHEQWDEASRVAYSVTPPGGT
                     YETERRFRRRDGTTFICRARGRRIDLDDPEQEWIWSFEDVTAEREADLRVQRALGELE
                     RAVAQRTAELEEAKARAQHLADHDPLTGLPNRRLLEDRLTQALALSHRNRKASAVMFV
                     DIDRFKTINDSLGHAVGDALLKVVASRLVEQLREGDTICRIGGDEFVVVLPEVKRSSD
                     VAHVAQKVIARLSQPLIVEERELLVTCSIGIAVYPDDGRDAESLIRNADAAMYHAKEL
                     GRANYQFFTEQMNHVASRRLQLENDLRRALGKDELRLHYQPIVETASARVVAHEALAR
                     WQHPERGLVSPTEFIQLAEETGVILKIGEWVLGEACRWSTFIGAERGMQIAVNLSARQ
                     FNDPQLPRMVARALKETGLPPRLLELEITESTAMQQTDVTLRTLKRLKALGVSIAIDD
                     FGTGYSSLSYLRRFPVDKVKIDRSFVSEVPRDHDQGAIIAAIVALAHALQIQVVAEGV
                     ETEAQRAFLASCRCDFIQGHLVGPPADADSAAQEYV"
BASE COUNT          815 a         1789 c         1878 g          881 t
ORIGIN      
        1 gggaagcctt gcccgccgag gcgtaattca cctttcccgg gttggctttg gcatgcgcga
       61 tcaacgcctg cgccgaagcc gccggcagcg acgggtgcac gatcagcacc gagtcgcttg
      121 aagcgagcgc cgtgatcggc gcgaagtcct tcagcgggtc gaagggcagc cgctcgtaaa
      181 ggtgcgggtt gctggtgacg atgctgaaaa gcgtaagcag cagcgtgtgg ccgtcggctg
      241 gcgatctggc aacctcggcc gcaccgatat tgcctgccgc gccgggccgg ttctccacga
      301 gcacgggccg cccgaggctc gcgccgagtc gctcggagag gatgcggccg aggacgtcga
      361 gcggtccgcc gggagggaac gggttcacca tgcgaaccgg cttcgacgga tagctctgcg
      421 ccgccgcttc gaagggaaga agcgccgcga cgagcgcgat gatcgcgaag cgcttcaagc
      481 gctcagcgct tggcttcgta cgccgcgatc gccttcttga ggtcggtgta ctccgcgcag
      541 gggaaaaagc acagcttgtc gagcgcgccc aggtgctcct tcgccttggc gacgttcccg
      601 accatgaggt acgcctcgcc gatgtactcg tgcgcgccgc ggtggttcgg gtcgatgcgc
      661 agcgcctcgt tgtagtgctt gaacaccagg ttcatgtccg ggctcgcgcc cttgcggatc
      721 gagtaggcgt agaggttgtg atagttggcg ttgttcgggt tggcggcgac cgcatctttc
      781 agcacggcgg ccgcgcccgt ccagtcctgc cgcttggtcg cttcggtcgc cttgtccagc
      841 accggatccc tggtctcggg agtgaccggg tcgctggcag cgagcacaat cccgggaacg
      901 gatagtatga gcgtcagcac gaagactttc atggtttctc cttcgaggct tgcaatggta
      961 ctcctcgcgg ttgcgctaac agcgggcgcg ccggccgcat tcccgcagcc gccgcaaacg
     1021 cgcgccgagg cgctcggcgc gttgcaaagc cccgacgcgg cgaggcgcgc cgaggcggtg
     1081 gtgtggatcg cgaaccgcgg cggcatggac gacgcgccgc tgctgcacga gcgcctgcgc
     1141 gacgaaagcg cgttcgtgcg cggcttcgcc gagcaggggc tgtggctgct ctggagccgc
     1201 tcgggcgacg ccgcggtcga ccgcctgatg gctctgggca tcgaggcgat gcaggccggg
     1261 cgccacagcg aagccatcgg tctgttttcg gaaattatcg agacgagacc cgacttcgcc
     1321 gagggatgga acaagcgcgc gacggtctac tacctcgccg gccagtatgg aaagtcgatt
     1381 gcggactgcc acgaggtgct gaagcgcaac cccggccatt tcggcgcgct gtcgggaatg
     1441 gggcagatct acctgcagct cgaggacgac gagcaggcgc tgcagtggta tcgccgtgcg
     1501 ctcgaagtga accccaacat gctcggcgtc gagatgagcc tcaggatgct cgaggagcgg
     1561 cttcgaaccc ggcgagcgcc tgggcgatcg acctagccgc ctcgcgcggg tgctcgaagg
     1621 ggaagagatg cccgccggcg accttgcgga agctgaactt cggccgcatg ccggcgaggc
     1681 ggattcggcg cacgacatcg gagtcggcgc cgccgatgaa cgccgccggc acttcgagct
     1741 gcgcgagatg gcgcatcatg tcgtgcggga tggtgcggta gatctcgtac tcgatccgcg
     1801 ggtcgatttt cagcccgagc ccgccgttcc tgctcacgag cccgtgggcc acatagtcgt
     1861 ccaggcactc cggagtgaaa ttgcgaaaca gctttctttc tcgtacggaa atgttccttc
     1921 gcctcctggg cgttgcgcca gtggctgcgc cggtcgcgcg tcgcgccggc gggcgtgacg
     1981 cggtcgacga tgccgagccg cttggtggcg ccgagcaggc tgccgcgaaa tgccccgatg
     2041 agcggcgcat cgaggagcac gatgccgcga aacagctccg gccggcggac tgcggcgagg
     2101 tagctgaggt acccgccgag cgaatggccg acgccgtaaa cggcttcacc gtggcgttcc
     2161 agctcgaaca gcagctggtc aacgagcaat ggccagcctt ctgtcggcgg atagcgcggg
     2221 tccatgccga tcgcctcgat ccaggtcacc tcatacgcga aagacagggg atgcaggacc
     2281 gcggcgtagc acgacgcggg aaacccgttc gcgtgcgaaa agtgaagctt cggcctagac
     2341 gtattcttgc gctgcgctgt ccgcgtcggc cggcgggccg acgaggtggc cctgaatgaa
     2401 gtcgcaccgg cacgaggcga ggaaggcgcg ctgcgcttcg gtctccacgc cctcggccac
     2461 gacctgaatt tgaagcgcgt gcgcgagcgc gacgatggcc gcgatgatgg cgccctggtc
     2521 gtgatcgcgc ggcacctccg agacgaagct gcggtcgatc ttcaccttgt cgaccgggaa
     2581 gcggcgcagg tacgagaggc tcgaatagcc ggtgccgaag tcgtcgatgg cgatcgacac
     2641 gccgagagcc ttgagccgct tcagcgtgcg cagcgtaacg tcggtctgct gcatcgcggt
     2701 ggactccgtg atctcgagct cgaggagccg cggcggcagc cccgtctcct tgagcgcccg
     2761 cgccaccatg cgcggaagct gcgggtcgtt gaactggcgt gccgagagat tcaccgcgat
     2821 ctgcatgccg cgctcggcgc cgatgaaagt ggaccagcgg cacgcctcgc cgagcaccca
     2881 ttcgccgatc ttcaggatga cgccggtctc ctcggcgagc tggatgaact ccgtcggaga
     2941 gacgagcccg cgctcgggat gctgccagcg ggcgagcgcc tcgtgcgcca ccacgcgcgc
     3001 gctcgccgtc tcgacgatcg gctggtaatg caggcgcagc tcgtccttgc cgagcgcgcg
     3061 gcgcaaatcg ttctcgagct gcagccgccg ggaggccacg tggttcatct gctcggtgaa
     3121 gaactggtag ttggcgcggc cgagctcctt cgcgtggtac atcgccgcgt cggcgttccg
     3181 gatcaggctc tcggcgtcgc ggccgtcatc cggatagacg gcgatgccga tcgagcaggt
     3241 gacgagaagc tcgcgctcct ccacgatgag cggctgcgaa agccgcgcga tcaccttctg
     3301 cgcgacgtgc gccacgtcgg aagagcgctt cacttcaggc agcacgacga cgaactcgtc
     3361 gccgccgatg cggcagatcg tgtcgccctc gcgcagctgc tccaccagcc ggctcgccac
     3421 caccttgagt agcgcgtccc cgaccgcgtg gccgagcgag tcgttgatgg tcttgaagcg
     3481 gtcgatgtcg acgaacatca ccgcgctcgc cttgcggttg cggtggctga gcgcgagcgc
     3541 ctgcgtgagg cggtcttcga ggagcctgcg gttcggcagc ccggtgagcg ggtcgtgatc
     3601 ggcgaggtgc tgggcgcgcg ccttcgcctc ctcgagctcc gcggtgcgct gcgcgaccgc
     3661 gcgctcgagc tcgccgagcg cgcgctgcac gcgcaggtcc gcctcgcgct ccgcggtgac
     3721 gtcctcgaag ctccagatcc attcctgctc gggatcgtcg aggtcgatgc gccggccgcg
     3781 cgcgcggcag atgaaagtgg tgccgtcgcg ccggcggaat cggcgctccg tctcgtaagt
     3841 gccgcccggc ggggtgacgc tgtaggcgac gcgactcgct tcgtcccatt gctcgtgcga
     3901 gtcgaacagc accgcggagc tctggccgat gagctcgccc gggcccgcgc cgaccatgtc
     3961 ctcgagaaag cggttgcagc gctggatcgc gcggctgcgc acgaacgcga tgccgaccga
     4021 ggcgttgtcg aggatgagct cctgctccgc aagcgcgcgc tggacccgct cctccgagtc
     4081 gtgctcctgc gtgatgtctt cgaagagcca caccgagcct tgcgacgggt cgcccggctg
     4141 cacggcgcgc ccggagatcg agcaccagat gagcgtgccg tccttgcgta cgtgccggcg
     4201 ctccacatac accgtctcgc cgcgccacac cggctcgtag agcgactcgc cggcgagctc
     4261 gtactcgcgg tcggtggcga acatgaagcg cgtcgagctg ttgatgagct cgcccgcgcc
     4321 gcagccgaag atctcctcga agcgccggtt gcagcgcacg atgcgccggt ccttgacgaa
     4381 gatgatgccg gtgaccgcgt tctgcagcac ggcatcctgc tcgcgcacca gccgctcgag
     4441 cgcctcgtcg gcgcgccggc gctcggtgat gtcctcgatc agccagacat ggcccttcga
     4501 cggatcgccc ggcgcgaccg cgcgcgcgga gatgcggcac cagaagccgg agccgtcctg
     4561 gcggcgcagc cactgctcgc gggtatgcat gcggccctgg tcgagctcgg cgatcacctg
     4621 cgagcgcagc tcgaactcct cgtcggtgaa gtacatctcg cgccacgaga cgccgatcgc
     4681 ctggccgtcg gcaaagccga acatttcctc gaagcgccgg ttgcagcgca cgagcttgcg
     4741 gctgcggctg atcgcgatgc cgaccaccac gttgtcgagg agcgcctgct gctcggcgag
     4801 cacgcgctgc agctcctcct cggcgcggcg ctgctcggtg acgtcctcca ccgtccacac
     4861 cgagcccttg tgcgggttct gcgggtcgag cgcgcgcccg tcggcgcggt tccagaaggt
     4921 gctgccgtcc ttgcgccggc gcagctcgat gcggcgcatg gtctgaccgc gcgccagcgc
     4981 gtcgtagatg ccttcggcgc gggcgaagtc ttccgcgcgc gggtagatcg ctccaaccga
     5041 ctggccgtgg agctctcccg ggccgtagcc gtacatctct tcgtagcggc ggttggcgcg
     5101 cacgaagcgt cggtccttga cgaacgcgat gccgaccgaa gcggtgctga gcagcgcttc
     5161 gtgctctcgc agcacgcgcg ccatctcgtc agcgtggcgg cggcgctcgg tgatgtcctc
     5221 gacgatccac acggtgccct ggtgcgtgtt cgccgggtcg atcgccttgg cgaccacgcg
     5281 gcagaggaac gtcgagccgt ccttgcgccg catttcccac tcggcgtcga gctggcgccc
     5341 cgccgacagg atcggggtgg cga
//
LOCUS       DASUNT010000202         2988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_727646, whole genome shotgun sequence.
ACCESSION   DASUNT010000202 DASUNT010000000
VERSION     DASUNT010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727646"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VFX94_03915"
     CDS             <1..468
                     /locus_tag="VFX94_03915"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX5767360.1"
                     /translation="ITGPARVFDDEQSALEAILAGKIKAGDVMVLRYLGPKGGPGMPE
                     MLAPTGALIGAGLGESVGLITDGRFSGGTWGMVVGHVAPEAYVGGTIALVKEGDSITI
                     DAKKRLLQLNVPAKELAARRKKWKAPNPRYKQGLLAKYMKLVSTASRGAITDG"
     gene            complement(465..1037)
                     /locus_tag="VFX94_03920"
     CDS             complement(465..1037)
                     /locus_tag="VFX94_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEX5767361.1"
                     /translation="MKSLAPEVLQFWFGQGAEYGKRHKRWFEKDPAFDAAVAQRFRGL
                     HEEMLAGKHRDWLEERRACLARVIALDQFPRHICRATAHAFSSDPLALEAAKRLVASG
                     WDRELLPVERMFAYLPFEHSESREDQERACELCEALLAFPEVADTHRYAVAHRDIIRR
                     FGRFPHRNEVLGRASTPEEVEFLKQPGSAF"
     gene            1240..>2988
                     /locus_tag="VFX94_03925"
     CDS             1240..>2988
                     /locus_tag="VFX94_03925"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX5767362.1"
                     /translation="MNRLRGETSPYLRQHAANPVDWYPWGPEALERARAEDRPILLSI
                     GYSACHWCHVMAHESFQDPGVAEVMNRHFVNVKVDREERPDLDQIYQTAHQMLARRTG
                     GWPLTLFLAPDGTPFFGGTYFPKEPRHGLPGFADLCERVAALWREKRAEIATQNAEVL
                     TALARTLPRSAALPNELDAAPVIELLEVLRANVDARFGGFGSAPKFPHPTDLELCLRN
                     GETAIPLTTLQRMCQGGIYDQLGGGFFRYSVDAEWLIPHFEKMLYDNGPLLGLLADAW
                     LVSADPLYKQCAAATAGWMMREMQSPEGGYYSSLDADSEHEEGKFYVWNREQAQRVLT
                     EQEWLAFSSHYGLDQPPNFENKHWHLHVAKPIEPGNESLVAIARQKLLEVREKRVRPG
                     RDEKVLVSWNALAIRGMVRAGRAFGDRSWIESARRAAVFVRSRMWRERRLAATYKDGR
                     AHLNAYLDDYALFIAAILELLQEEFSVEELDFALELAEVLLEQFEDRAQGGFFFTAHE
                     HERLIHRPKPGHDHATPSGNAVAAWALNRLHALTGEPRYAAAAARTLALFYPAMREHP
                     GGFAMMAIALEEQLRQP"
BASE COUNT          509 a         1000 c          979 g          500 t
ORIGIN      
        1 atcaccggcc cggcgcgcgt gttcgacgac gagcagtcgg ccctcgaggc catcctggcc
       61 ggcaagatca aggccggcga cgtgatggtg ctgcgttacc tcggccccaa gggtggcccg
      121 ggcatgcccg agatgctggc ccccacgggt gcgctgatcg gcgcgggcct cggcgaaagc
      181 gtgggcctga tcaccgacgg ccgcttctcg ggcggcacct ggggcatggt cgtcgggcat
      241 gtggcgcccg aggcctatgt cggcggcacc atcgcgctcg tcaaggaagg cgactccatc
      301 accatcgatg cgaagaagcg gctgttgcag ctcaatgttc cggccaagga gctcgccgcg
      361 cggcgcaaga agtggaaggc gcccaatccg cgctacaagc aggggctcct cgccaagtac
      421 atgaagctcg tatcgaccgc ctcgcgcggt gcgatcacgg acggctagaa cgcggacccg
      481 ggctgcttca ggaattcgac ctcttccggg gtgctcgcgc ggcccagcac ctcgttgcga
      541 tgcggaaagc gcccaaagcg gcggatgatg tcgcggtggg ccaccgcgta gcgatgcgta
      601 tcggctactt ccgggaaagc gaggagcgct tcacacagct cgcacgcccg ttcctggtcc
      661 tcgcgcgatt cgctgtgctc gaacggcagg tacgcgaaca tacgttccac gggtagcagc
      721 tcccggtccc agccgcttgc cacaagccgc ttcgcggctt cgagcgcgag aggatcgctc
      781 gaaaaggcat gcgctgtcgc gcgacaaatg tggcgcggga actggtcgag cgcgatgacg
      841 cgtgcgaggc aggcacgccg ctcctcgagc cagtcgcggt gcttgcctgc gagcatctct
      901 tcgtgcaaac cgcggaagcg ctgcgccacg gcggcgtcga acgcagggtc tttttcgaac
      961 cagcgcttgt gccgcttgcc gtattcggca ccttggccga accagaactg caacacctcg
     1021 ggcgctaagc ttttcatcga ccgcgaccga ataagacggg agactccatg atcgctcatt
     1081 tggactcggc gtgggccggg aacgcatagc ccggttcctg cgtcgtactg atgtgcaggc
     1141 gcaatcacaa gcgtgttccc ggtgctgctc ctcgcggata tactgggcac gccaaggtct
     1201 ttccttttgc gcgcccgatg aacaagtgag ccgcgtggcg tgaaccgact tcggggcgag
     1261 acttcgccct acctccggca gcacgcggca aatccggtcg actggtatcc ctggggcccc
     1321 gaggcgctcg agcgcgcccg cgccgaagac aggccgatcc ttctttcgat cggctactcg
     1381 gcatgccact ggtgccacgt gatggcgcac gagagtttcc aagaccccgg cgtcgcggag
     1441 gtgatgaacc ggcacttcgt caacgtgaag gtggatcgcg aagagcggcc cgacctcgac
     1501 cagatctacc agacggcgca ccagatgctc gcccgccgaa ccgggggctg gccgctcacc
     1561 ctcttcctcg cccccgacgg tacgccgttt tttggcggca cttattttcc caaggagccg
     1621 cgtcatggcc tgcccgggtt cgcggacctg tgcgagcgcg tcgccgctct ctggcgcgaa
     1681 aaacgcgccg aaatcgcgac gcagaatgcc gaggtgctga ccgcgctggc gcgcacgctg
     1741 ccgcgctcgg ccgcgctgcc gaacgagctg gacgccgccc cggtcatcga gctgctcgaa
     1801 gtgctcaggg ctaacgttga cgcccgcttc ggcggcttcg ggtcagcgcc gaaatttcct
     1861 cacccgacgg acctggagct ttgcctgcgc aacggcgaaa cggcaatccc cctcaccacg
     1921 ctccagcgaa tgtgccaagg cggcatctac gaccagctcg gcggcggctt ttttcgctac
     1981 agcgtggatg ccgagtggtt gatcccgcac ttcgagaaga tgctctacga caacgggccg
     2041 ctgcttgggc tgctggccga cgcctggctc gtgagcgctg acccgctcta caagcaatgt
     2101 gcggcggcca ccgccggctg gatgatgcgc gagatgcaat ctcccgaagg cggctactac
     2161 tcctcgctcg atgccgacag cgagcacgag gagggtaagt tctatgtctg gaaccgcgag
     2221 caggcgcagc gcgttctcac ggaacaggaa tggctcgctt tctcctcgca ctatgggctc
     2281 gaccagccgc cgaacttcga gaacaagcac tggcacttgc acgttgcgaa gcccatcgag
     2341 cctggaaacg aaagtcttgt ggcaatcgcc aggcagaagc ttttggaggt gcgcgaaaag
     2401 cgcgtgcggc cgggacggga cgagaaggtg ctcgtttcat ggaatgcgct cgccattcgg
     2461 ggcatggtgc gcgcagggcg ggcgttcggg gaccggtcct ggatcgagtc cgcgcgccgg
     2521 gcggccgtgt tcgtccgctc gaggatgtgg cgcgagcggc gcctcgcggc gacgtacaag
     2581 gacggccgcg cgcatctcaa cgcttacctc gacgactacg cgctcttcat cgccgccatc
     2641 ctcgagttgc tgcaggaaga gttctccgtg gaggagctcg acttcgcgct cgagcttgcc
     2701 gaggtgcttc tcgagcagtt cgaggaccgc gcgcagggcg ggtttttctt caccgcgcac
     2761 gagcacgagc ggctgatcca ccggcccaag ccgggccacg accacgccac tccctcgggc
     2821 aacgccgtcg ccgcttgggc tctgaaccgc ctgcacgcac tgacaggcga gccgcgatat
     2881 gccgccgccg cagcgcgcac gctcgcgctt ttctaccccg caatgcgcga gcaccccggc
     2941 ggcttcgcca tgatggcgat tgcgctggag gagcagctgc gccagcca
//
LOCUS       DASUNT010000203         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_465897, whole genome shotgun sequence.
ACCESSION   DASUNT010000203 DASUNT010000000
VERSION     DASUNT010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465897"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..983
                     /gene="ftsY"
                     /locus_tag="VFX94_03930"
     CDS             81..983
                     /gene="ftsY"
                     /locus_tag="VFX94_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX5767363.1"
                     /translation="MVSPSWAARLKAGLARTRDVLNTPVSELFTRRKIDEALYEELET
                     ALLQADCGVAAAQSLIAELRRKKVADGEALKAALKDAIAELLSPLERRLTIEKAKPFV
                     IMIAGVNGSGKTTSIGKLAKWLQAQGKSVLLAAGDTFRAAAREQLVAWGARNAVPVIA
                     QESGDPGAVVFDALSAARARNIDVVIADTAGRLPTQLHLMEEIKKVKRVAAKALEGAP
                     HEVLLVLDANTGQNSLTQVKAFDAALGLTGLVLTKLDGTAKGGVVCAIAKEKPVPLLF
                     VGVGEGIDDLRPFAARDFAEALVG"
     gene            976..1620
                     /locus_tag="VFX94_03935"
     CDS             976..1620
                     /locus_tag="VFX94_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5767364.1"
                     /translation="MVSFSGVAKRYPGQQALYDVSFSIERGELAFITGRSGAGKSTLL
                     KLIPAIERPSAGTVIVHGQNVGAIKRAAIPYLRRNIGLVFQDRKLLYDRSVYDNVMLP
                     LAFSAHPPKEAARRARAALDKVGLAARERANPVELSGGEQQRLAIARAVVNRPALLVA
                     DEPTANLDAESAAHVLELFAAFNGVGVTVLIATHDDALVARYGKRVLHLEAGRL"
     gene            1620..2489
                     /locus_tag="VFX94_03940"
     CDS             1620..2489
                     /locus_tag="VFX94_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX5767365.1"
                     /translation="MSTWLRHHRQALASAFRRLSVLNALVIGIALALPAGGYALLSSL
                     RPAGERLALEPRISLFLQPQAPRSHAEALGRTLRGDPRISAVRFVPREEALKELSAVQ
                     GLSEVIAALGRNPLPDAFVVTSNADIAGELARLPGVAHVQSDAVWARRMAAAARLTGR
                     ALTLLAALLGAGLVAVTFNTIRLQILTRRDEMEVSKLLGATDAFIRRPFYYLGLLQGL
                     AGGALALALVAAALAVLNREVVALADSYGSAFRFSFLPVEEALALVLLAGVLGLLGAQ
                     LSMSRHLRAIEPR"
BASE COUNT          371 a          869 c          837 g          419 t
ORIGIN      
        1 cgatccgcga ctgcaatgcg ccggccgcga tactcgccgc cgatgatccg tattcgtcct
       61 ttgttagaat ccaccgaccc atggtatctc cctcttgggc ggcgcgcctc aaggcaggcc
      121 tcgcgcgcac tcgggacgtc ctcaacactc ccgtttcgga gctcttcacc cggcgcaaga
      181 tcgacgaggc gctctatgaa gagctcgaga ccgcgcttct ccaggctgac tgcggcgtcg
      241 ccgccgcgca gtcgctcatc gccgagctga gaagaaagaa ggtggccgac ggcgaggcgc
      301 tgaaagccgc tttgaaggac gccattgccg agctcttgtc acccctggag agaaggctca
      361 cgatcgagaa agcgaaaccg tttgtgatca tgatcgccgg cgtgaacggc tcggggaaaa
      421 ccacctcgat cggcaagctc gcgaaatggc tgcaggcgca ggggaaaagc gttcttcttg
      481 ccgccggcga cactttccgc gcggccgcgc gcgagcagct cgtcgcctgg ggcgcgcgca
      541 atgcggtgcc cgtgatcgcg caggagtccg gcgatccggg cgcggtggtg ttcgacgcgc
      601 tttccgcggc gcgcgcgcgc aacatcgacg tcgtcatcgc cgacaccgcg ggccgcctgc
      661 cgacgcagct gcacctcatg gaagagatca agaaggtaaa gcgggtggcg gcgaaggcgc
      721 tcgagggcgc gccgcacgag gtgctcctcg tgctcgacgc gaacacggga cagaactccc
      781 tcacgcaggt gaaggccttc gacgccgcgc tcgggctgac gggcctcgtt ctcaccaagc
      841 tcgacggcac ggcgaagggc ggcgtcgtgt gcgctatcgc gaaggagaag cccgtgccgc
      901 ttctcttcgt cggcgtggga gaaggcatcg acgacctgcg gcctttcgcc gcgcgcgatt
      961 tcgccgaggc gctggttggt tagcttttcc ggcgtcgcga agcgctaccc tggccagcag
     1021 gcgctctacg acgtgagctt ctcgatcgag cgcggcgagc tcgccttcat caccgggcgc
     1081 tcgggcgccg gcaagtcgac gctgctcaag ctcatcccgg cgatcgagcg tccgagcgcc
     1141 ggcacggtga tcgtgcacgg ccagaacgtc ggcgcgatca agcgcgcggc gatcccctac
     1201 ctgcggcgca atatcggact cgtgttccag gaccggaagc tgctctacga ccgcagcgtc
     1261 tacgacaacg taatgctgcc gctcgctttt tccgcgcatc cgccgaagga ggccgcacgg
     1321 cgtgcgcgcg cggcgctcga caaggtcggc ctcgccgcgc gcgagcgcgc aaatccggtg
     1381 gaactttcgg gcggggagca gcagcgcctc gccatcgcgc gcgccgtggt gaaccggcca
     1441 gcgctgctcg tcgccgacga gccgacggca aacctcgacg ccgaatcggc ggcgcacgtg
     1501 ctggagctct ttgccgcatt caatggggtc ggcgtgacgg tgctcatcgc cactcacgac
     1561 gatgcgctcg tcgcgcgcta cggcaagcgc gtgctgcacc tcgaggcagg acggctgtga
     1621 tgagcacctg gctgcgccac catcggcagg ctctcgcgtc ggctttccgg cgcttgagcg
     1681 tgctgaatgc cctggtgatc gggatcgcgc tcgcgctgcc ggcgggcggc tacgcgcttc
     1741 tctcgagcct gcgcccggcg ggcgagcggc tcgcgctcga gccgcgcatc tcgcttttcc
     1801 tccagcccca ggcgccgcgc tcgcacgccg aagcgctcgg cagaacgctg cgcggcgacc
     1861 cccgcatcag cgccgtgcgc ttcgtgccgc gcgaagaagc cctgaaggag ttgagcgccg
     1921 tgcagggcct gtcggaggtg atcgcggcgc tcggccgcaa tccgctgccc gatgccttcg
     1981 tcgtcacgtc gaatgccgac atcgccggcg agctcgcccg gctgccgggc gtggctcatg
     2041 tccagtcgga cgcggtgtgg gcgcgtcgca tggccgcggc agcccggctc accgggcgcg
     2101 cgctcacttt gctcgccgcg cttctcggcg cggggctcgt cgccgtgact ttcaatacca
     2161 tccggcttca gatcctcacc cggcgcgacg aaatggaagt gtccaagctc ctgggagcga
     2221 ccgacgcctt cattcgccgg ccgttctatt acctcgggct cttgcaaggc ctggccggcg
     2281 gcgcactggc cctcgcgctc gtggctgcag cgcttgcggt cctcaaccgg gaagtcgtag
     2341 cgctggccga ctcctacggc tcggcctttc gattctcttt cctgcctgtc gaggaagcgc
     2401 tggccctggt tctcctcgcc ggggtcctgg ggttgctagg cgctcagctc tctatgagcc
     2461 gccatctgcg ggccatagaa ccccgctaga gattgc
//
LOCUS       DASUNT010000204         4107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_641107, whole genome shotgun sequence.
ACCESSION   DASUNT010000204 DASUNT010000000
VERSION     DASUNT010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641107"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VFX94_03945"
     CDS             <1..410
                     /locus_tag="VFX94_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HEX5767366.1"
                     /translation="GSDYQVVKLAVMGEVKTHFRPEFVNRIDEIVVFHALGEEHIRSI
                     ARIQLRILESRLAKMDYGLQVADAALAELAKVGFDPVYGARPLKRAIQSEIENPLAKA
                     ILEGRFAPKDTVVVDYRGGKMVFEKKKSVKAAA"
     gene            complement(459..2024)
                     /locus_tag="VFX94_03950"
     CDS             complement(459..2024)
                     /locus_tag="VFX94_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5767367.1"
                     /translation="MKRIFAALASAVLAVSCATTREESLVDRAVDSMGGYERLAGIKS
                     FTFKGNAKYWEPEQSDAPGGEMRFANESSFEGFTDVASGSTRIDWVRNFAYPAPRTFT
                     FSEIVTREAGYVLGVDSNGRNAQNLKMSPPAHSMSGYRLAAAQRELRRAGSAGLVLAM
                     KRFPDRVQPAADIAGQPAVSFDGFLVAFDPATGLPARVRSLDYDNIWGDVTYDVAFSN
                     WRDIGGVRIPMSRKAELNGRTVTEMSFTDLQINQPVDTARLQVPAEIRDGAAKPASRN
                     VPYQWVMRRQFIGTYMDSDNVSYDTRASQGLRLQEIAAGVHQVQGGTHNSLVVEMSDH
                     LIVLDAPITDAQSLWLVGATRAQFPGKPIRWLALTHHHMDHAGGLRGILAEGGVTLVV
                     GQGAGAHFRRVLEAPATRNPDLPARSYHMTPILEVPESHVMSDSTGRQVIVYVMDNPH
                     AKGMLMGWVPHARIGYVTDIWTPGPPLPAKPNPGLASVVNTVKRGGLQPERFAGGHGS
                     TADYQTLVRLVSQ"
     gene            complement(2082..2420)
                     /locus_tag="VFX94_03955"
     CDS             complement(2082..2420)
                     /locus_tag="VFX94_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX5767368.1"
                     /translation="MFALSRLEWLAFAAALLLCPGGSPLGAGPPANTDATAPSSTARS
                     LFDRLDRNRDGYLNAAELATEEAKRGNWIAIDRDRDGRIGRGEFGLVGASEPASSAAA
                     GGTRAPKKPE"
     gene            complement(2477..3094)
                     /locus_tag="VFX94_03960"
     CDS             complement(2477..3094)
                     /locus_tag="VFX94_03960"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX5767369.1"
                     /translation="MNTALRLVAFLAGLLAACSSAFAQSEDDPIILVAHPAFRDLEYR
                     QTVLIAAPAPNGGHVGVILNRPTRRSLGSLFPEHEPSKRVADPVYYGGPFSRGALVAL
                     VKADSAPGAGSVLLMKNLYLAFRANTIDHVIETTPNEARYFVGYVGWRPGELKSEIER
                     GLWSVHGADLDTIFRKDTEGLWDELQSQSRRIRAAVPAEALALLR"
     gene            complement(3116..3679)
                     /locus_tag="VFX94_03965"
     CDS             complement(3116..3679)
                     /locus_tag="VFX94_03965"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX5767370.1"
                     /translation="MRRSLAVLALCIASCAAAQMQHEPPNGLLLVAKPGLGDPNFSQT
                     VVLVSQGADGSTVGVILNRPTPAKHEQTGAPVFSGGPVMREVKVALFRSERAPEAAAF
                     HVLKGIYLTMHPENLQKLFGDPAARDGDYRVFKGFAGWAPGQLQSELARGDWFVLPAS
                     AALIFRSDTSGMWEELVRKARGRVAMR"
     gene            complement(3676..>4107)
                     /locus_tag="VFX94_03970"
     CDS             complement(3676..>4107)
                     /locus_tag="VFX94_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEX5767371.1"
                     /translation="KEYTAKRRTVSFGLDYDFSSNELAPAAELPGFLDALRTRAAGWL
                     GVASDRIRHALVSEYQPGTALGWHRDVPQFELVCGISLASSCRMRFRPFPPRKDRKSA
                     LALELAPRSAYVLRGDARWRWQHSIAPTRTLRYSITFRTLA"
BASE COUNT          688 a         1326 c         1441 g          652 t
ORIGIN      
        1 cgggcagcga ctaccaggtg gtgaagctcg ccgtgatggg cgaggtgaag acccatttcc
       61 ggccggaatt cgtgaaccgg atcgacgaga tcgtagtgtt ccacgcgctc ggagaagagc
      121 acatcaggtc catcgccaga attcagttga gaatcctgga aagtcgtctg gcgaaaatgg
      181 actacgggct gcaggtcgcg gacgcggcgc tcgccgagct cgccaaggtg ggattcgacc
      241 cggtgtacgg cgcgcggccg ctcaagcgcg ccatccagtc ggaaatcgaa aacccgctag
      301 cgaaggcgat cctcgaaggc cgcttcgcgc cgaaggacac cgtcgttgtc gactaccgcg
      361 gcggcaagat ggtcttcgag aagaagaaga gcgtcaaagc cgcggcgtag ggggtcagga
      421 ccctttttcc gggcgctacg cgaagggtcc tgatcccttt actggctgac gagtctcacc
      481 aacgtctgat agtccgccgt agagccgtgg ccgccggcga agcgctccgg ctgcaagccg
      541 ccgcgtttca cggtgtttac gacggaggcg aggccggggt tgggctttgc gggaagcggc
      601 ggtccgggcg tccagatgtc ggtcacgtag ccgatccgcg cgtgcggcac ccagcccatc
      661 agcatgccct tggcgtgcgg gttgtccatc acgtagacga tcacctgccg gcccgtcgaa
      721 tcggacatga cgtggctctc gggcacttcg agaatggggg tcatgtggta cgagcgtgcg
      781 gggaggtcgg ggttgcgcgt ggccggggct tcgagcacgc ggcggaaatg cgcgcccgcg
      841 ccctggccga cgaccagcgt cacgccgccc tcggcgagaa tgccgcgcaa gccgccggcg
      901 tggtccatgt gatggtgggt cagcgccagc cagcggatcg gcttgcccgg gaactgggcg
      961 cgcgtcgcgc cgacaagcca gagcgactgg gcgtcggtga ttggcgcatc gagcacgatg
     1021 agatggtcgc tcatctccac caccaggctg ttgtgcgtgc cgccctgcac ctgatgcacg
     1081 cccgccgcga tctcctggag ccggaggccc tgcgaggcgc gcgtgtcgta gctcacgttg
     1141 tcggaatcca tgtaggtgcc gatgaactgc cgtcgcatca cccactggta cggcacgttg
     1201 cgcgatgccg gctttgccgc accgtcgcga atctcggcgg gcacctggag ccgcgcggtg
     1261 tcgaccggct ggttgatctg gaggtcggtg aagctcatct cggtgacggt gcgcccgttg
     1321 agctcggcct tgcggctcat cgggatcctg accccgccga tgtcgcgcca gttggaaaac
     1381 gccacgtcgt acgtcacgtc accccagatg ttgtcgtaat cgaggctgcg caccctcgcg
     1441 ggcaggccgg tggcgggatc gaacgcgact aggaaaccgt cgaacgacac cgcgggctgg
     1501 ccggcgatat cggcggcggg ctgcacgcgg tcgggaaatc tcttcatcgc gagcacgagc
     1561 ccggccgatc ccgcgcgccg cagctcgcgc tgagccgcgg cgagacggta acccgacatg
     1621 gaatgcgccg gcgggctcat tttcaggttc tgcgcgttgc gcccgttgct gtccacgccg
     1681 agcacgtacc cggcctcccg cgtcacgatc tcgctgaacg tgaacgtgcg cggcgccggg
     1741 taggcgaaat tgcgtaccca gtcgatgcgc gtcgagcccg acgcgacatc ggtgaagccc
     1801 tcgaagctcg actcgttggc gaagcgcatc tcgccgcccg gcgcgtcgga ttgctcgggt
     1861 tcccagtact tggcgttgcc cttgaacgtg aagctcttga tccccgcaag gcgctcatag
     1921 ccgcccatgg aatcgaccgc gcggtcgacg aggctctcct ctcgggtggt ggcgcaggaa
     1981 acggcaagaa cggcgctggc gagcgccgcg aagatgcgct tcatgacagt cctcccagga
     2041 tgtaacgaaa ccagcctagg cgaacaggaa cgcgtttaca actattccgg ctttttcggc
     2101 gcccgggtgc cgcccgcggc ggcgctggag gcgggctcgc tggcaccgac caggccgaat
     2161 tcgccgcggc cgatgcggcc gtcgcggtcg cggtcgatcg caatccagtt gccccgcttt
     2221 gcctcctcag tcgcgagctc ggcggcgttc aggtagccgt cgcggttgcg gtcgaggcga
     2281 tcgaagagcg agcgcgcggt ggacgaaggc gcggtagcgt cggtattggc cggcggcccc
     2341 gcgccgagcg gcgagcctcc cgggcacagg agaagcgctg cggcgaaggc aagccattcg
     2401 aggcgggaaa gcgcgaacat ccgggccaga atagcccggc aaagtgcagc ttacgtgcgt
     2461 tgcgggtgtg agccgttcag cgcaggagcg cgagcgcttc ggccggaacg gcggcgcgga
     2521 tgcggcgcga ctgcgactgc agctcgtccc aaaggccttc ggtgtccttg cggaaaatgg
     2581 tgtccaggtc ggcgccgtgc accgaccaca gcccgcgctc gatctcgctc ttcagctcgc
     2641 cggggcgcca gccgacatag ccgacgaaat agcgcgcctc gttcggcgtc gtctcgatca
     2701 cgtggtcgat ggtgttggcc cggaacgcga gataaagatt tttcatgagc agcaccgagc
     2761 cggcgccggg ggcgctgtcg gccttgacca gcgcgacgag ggctccgcgc gagaacggcc
     2821 cgccgtagta aaccgggtcg gcgaccctct tcgacggctc gtgctctggg aaaaggctgc
     2881 cgagcgagcg ccgggtcggg cggttgagga tcacgcccac atggccgccg ttcggcgccg
     2941 gagccgcgat cagcaccgtc tggcggtact cgaggtcgcg gaacgccgga tgcgcgacca
     3001 gaatgatggg gtcgtcctcg ctttgggcga aggccgaact gcaggcggca aggaggcccg
     3061 ccagaaaagc gacgaggcgc agggcggtgt tcatggggcc agtatacgtt tcctttcagc
     3121 gcatcgctac acggccacgg gcttttctga caagctcttc ccacatgccg gacgtgtcgc
     3181 tgcgaaagat gagcgcggcg ctcgcgggaa gcacgaacca gtcgccgcgc gcaagctcgc
     3241 tctgcagctg tccgggcgcc cagccggcga atcctttgaa aacgcggtag tcgccgtccc
     3301 gcgccgccgg atcgccgaac agtttctgaa gattttccgg atgcatggtg aggtatatcc
     3361 ctttcagaac atgaaaggct gcggcctcgg gcgcgcgctc ggagcgaaac aacgccacct
     3421 tcacctcgcg catcaccggg ccgccggaaa aaaccggcgc gcccgtctgc tcatgcttcg
     3481 ccggggtcgg gcggttgagg atcaccccga cggtgctgcc gtcggcgccc tggctgacga
     3541 gcaccaccgt ctggctgaag ttcgggtcgc cgaggccggg cttggccacg agcagcagcc
     3601 cgttcggcgg ctcgtgctgc atctgcgcag ccgcgcacga ggcgatgcaa agcgcaagca
     3661 cggcgagcga gcgcctcatg ccagcgtacg gaacgtaatc gagtagcgca gcgtccgggt
     3721 cggcgcaatg ctgtgctgcc agcgccagcg cgcgtcgccg cgcaggacat aggccgagcg
     3781 cggcgcgagc tcgagcgcga gcgcgctctt gcggtccttg cgcggcggga aaggccggaa
     3841 acgcatgcgg caggagcttg cgagcgaaat cccgcacacg agctcgaact gcggcacgtc
     3901 gcgatgccag ccgagggccg tccccggctg gtattccgac acgagcgcgt gccttattcg
     3961 gtcggacgca actccgagcc accctgccgc ccgggtgcgc agcgcgtcga gaaacccggg
     4021 cagctcggcg gccggagcga gctcgttgct ggagaagtcg tagtcgagtc cgaagctcac
     4081 ggtgcgacgc ttcgccgtgt actcctt
//
LOCUS       DASUNT010000205         2834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_553286, whole genome shotgun sequence.
ACCESSION   DASUNT010000205 DASUNT010000000
VERSION     DASUNT010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553286"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /gene="gatB"
                     /locus_tag="VFX94_03975"
     CDS             <1..448
                     /gene="gatB"
                     /locus_tag="VFX94_03975"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014674.4"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEX5767372.1"
                     /translation="ADVIAFPSLYESTAHAIVGDLAAYLNEHGRPFSESPVGPRDVAL
                     IQTRVADGTISGKISKELLKAKIQGEKGSIDELIDKRGLRQISDAGTIEKLVDEVLAK
                     NAKQVEDYRAGKEKAFNSLVGQVMKATQGRANPAQVNEILRKKLKS"
     gene            complement(445..1077)
                     /locus_tag="VFX94_03980"
     CDS             complement(445..1077)
                     /locus_tag="VFX94_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767373.1"
                     /translation="MTPITAIFAAAALLASLAAHAQQKEQYTYRCTGKDGKKYYGQTI
                     PQSCLGQPLELINKQGIVVKRIDPEGDEKARLAKEAEAEKKRELEAAQKESMRRNRAL
                     LATYTSEKDIEESRKRDLATHQRSVQEVESRIAEIKKRQAQHQKELAAFEEPGKGPPP
                     ARLKEEITNAEIDLKAQQSLLDAKKKEAVTINARYDEDRRRYREAIGKPR"
     gene            1136..1828
                     /locus_tag="VFX94_03985"
     CDS             1136..1828
                     /locus_tag="VFX94_03985"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX5767374.1"
                     /translation="MHFADKAFVAETTAKMLLEVKAVLFFSDKPFVFTSGWASPVYID
                     CRKLIFYPRIRTQLVDFAAGMLARDVGFEQFDVVAGGETAGIPYAAWIAERLGLPMQY
                     VRKKPKGFGRNAQIEGDVREGARTLLVEDLTTDGRSKINFCQALRAAGAIVDHVFVNF
                     YYDIFPESKKILADLDVRLHYLATWWDVLEVVKKGGYLESKQLAEVESFLHAPAQWSA
                     AHGGVSAFPAAT"
     gene            1825..2598
                     /locus_tag="VFX94_03990"
     CDS             1825..2598
                     /locus_tag="VFX94_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX5767375.1"
                     /translation="MRIVTLNVNGIRSAASKGLFRWLAAQRADVVCLQEMKCHEIDLD
                     ARLHGLKVYQTCHAFAKKKGYSGVSLYSRKTPDEVRRGFASREFDAEGRYVEARFGKL
                     WVVSMYMPSGSAGPHRQVSKFRFLKHFLVHLQRLRQRNQEIILCGDWNIAHKEVDLKN
                     WRSNQKNSGFLPEERAWLTRVFDELGFVDVFRRLNDRPDQYTWWSNRGQAWKKNVGWR
                     IDYQVATPGIAALARRESIYKAKRFSDHAPLIIDYEHEL"
     gene            2588..>2834
                     /locus_tag="VFX94_03995"
     CDS             2588..>2834
                     /locus_tag="VFX94_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767376.1"
                     /translation="MSFSRLPAGLRLYLEPAPLAALLLGVSSGFTFAMIGATLSTRLA
                     QHGITRSAVTAFALTFLAYNFKFLWAPIVDNVRLPLIG"
BASE COUNT          481 a          892 c          906 g          555 t
ORIGIN      
        1 cgcggatgta atcgcttttc cctcgctcta cgagtctacg gcgcatgcga tcgtggggga
       61 cttggccgca tatttgaatg agcacggtcg accattttcg gagagtccgg tcgggccccg
      121 ggatgtcgcg ctgatacaga ctagggtcgc agacggcacg atatcgggaa agatctcgaa
      181 ggaacttcta aaagcgaaaa ttcaaggcga aaaaggctcg atcgacgagc tcatcgacaa
      241 gcgcggccta cgccagatca gcgacgccgg gacgatcgag aagctggtgg acgaggtgct
      301 ggcgaagaat gccaagcagg tcgaggacta ccgtgccggc aaggagaagg ccttcaactc
      361 gctcgtcgga caggtgatga aggcgaccca gggcagggcg aaccccgccc aggtcaacga
      421 gattctgcgc aaaaagctga aaagctagcg cggcttgccg atcgcctcgc ggtagcggcg
      481 tcgatcctcg tcgtagcgcg cgttgatcgt gaccgcctct ttcttcttcg cgtcgagcag
      541 actttgctgc gctttcaggt cgatctcggc gttggtgatt tcttccttca ggcgcgcggg
      601 cggcggtccc ttgccgggct cctcgaacgc ggcgagctcc ttctggtgct gcgcctggcg
      661 cttcttgatc tccgcgatgc gactctccac ctcctggacg ctgcgctggt gggtcgcaag
      721 atcgcgcttg cgcgactcct cgatgtcctt ttcgctcgta taagtggcga gcagtgcgcg
      781 gttgcggcgc atcgattcct tttgcgccgc ctcgagctcg cgtttcttct cggcctcggc
      841 ctccttggcg aggcgcgcct tttcgtcacc ctcggggtcg atgcgcttca cgacgattcc
      901 ctgtttgttg atgagctcga gcggctggcc gaggcagctt tgcggaatcg tctgaccgta
      961 gtatttcttc ccgtccttgc cggtgcagcg gtaggtgtac tgctctttct gctgagcgtg
     1021 cgcggcgagc gaagccagca gcgcggctgc ggcgaaaatt gcggttattg gagtcatcgc
     1081 caccctcccg aagcgcgaat ggcgggcatt ctacctgcta gaatccgcgc cgcccatgca
     1141 cttcgccgac aaggccttcg ttgccgagac cacggccaag atgctgctcg aggtcaaggc
     1201 tgtgctgttt ttctcggaca agccgttcgt tttcacttcg ggttgggcga gcccggtgta
     1261 catcgactgc cgcaagctca tcttctatcc acgcatccgc acgcagctgg ttgacttcgc
     1321 cgccggcatg ctggcgcgcg acgtcggctt cgagcagttc gacgtggtcg cgggaggcga
     1381 gaccgccggc attccctacg cggcgtggat cgccgagcgc ctcggcctgc cgatgcagta
     1441 cgtgcgcaaa aagccgaaag gcttcggccg caacgcgcaa atcgagggcg acgtgcgcga
     1501 aggcgcgcgc acgctcctcg tcgaggatct caccaccgac ggccgctcga agatcaattt
     1561 ttgtcaggcg ttgcgcgcgg cgggcgccat cgtggaccac gtcttcgtga atttctacta
     1621 cgacattttt cccgagtcga agaagatcct cgccgacctg gacgtccggt tgcactacct
     1681 cgccacgtgg tgggacgtgc tcgaggtggt gaagaagggc ggctacctcg agtcgaagca
     1741 gctcgccgag gtggaaagct tcctgcacgc gcccgcgcag tggtccgccg cgcatggcgg
     1801 ggtttccgcc tttcccgctg cgacttgagg atcgtcactc tcaacgtcaa cggaatccgc
     1861 tcggcggcaa gcaaagggtt gttccgctgg ctcgccgccc agcgcgccga cgtggtgtgc
     1921 ctgcaggaga tgaagtgcca cgagatcgac ctcgacgcga ggctgcacgg cctcaaggtc
     1981 taccagacct gccatgcgtt cgcgaagaag aagggctaca gcggcgtctc gctctatagc
     2041 cgcaagaccc cggacgaggt gcgccgcggc ttcgcgagcc gcgagttcga cgccgagggc
     2101 cgctacgtgg aggcgcgctt cgggaagctt tgggtggtct ccatgtacat gccgtccggc
     2161 tcggccgggc cgcaccggca ggtgtcgaaa ttccgctttc tcaagcactt cctggttcat
     2221 ctgcagcggt tgagacagcg caaccaggaa atcatcctgt gcggcgactg gaacatcgcg
     2281 cacaaggagg tcgacctcaa gaactggcgc tcgaaccaga agaattcggg ctttctgccc
     2341 gaggagcgcg cgtggcttac gcgcgtgttc gacgagctcg gcttcgtcga cgtgttccgc
     2401 aggctgaacg accggcccga ccagtacacc tggtggtcga accgcggcca ggcctggaag
     2461 aagaacgtgg gctggcgcat cgactaccag gtcgcgacgc ccggcatcgc ggcgctcgcg
     2521 cgcagggaat cgatctacaa ggcgaagcgc ttctccgacc atgcgccgct catcatcgac
     2581 tacgagcatg agctttagca ggctgccggc gggcctgcgg ctgtacctgg agccggcgcc
     2641 gctcgcggcg cttttgctcg gagtttcatc gggcttcacg ttcgcgatga tcggcgcgac
     2701 gctcagcacg cgcctcgccc agcacggcat tacgaggagc gcggtgacgg ccttcgcgct
     2761 cacctttctc gcctacaact tcaagttcct gtgggcgccg atcgtcgaca acgtgcgcct
     2821 gcccctgatc ggcc
//
LOCUS       DASUNT010000206         5099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_379037, whole genome shotgun sequence.
ACCESSION   DASUNT010000206 DASUNT010000000
VERSION     DASUNT010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379037"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1983)
                     /locus_tag="VFX94_04000"
     CDS             complement(<1..1983)
                     /locus_tag="VFX94_04000"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HEX5767377.1"
                     /translation="MSEHKGNGESEYGSDAIKVLKGLEAVRKRPGMYIGDTSDGTGLH
                     HMVFEVVDNAVDEALAGFCDEIIVTIHTDNSVSVLDNGRGIPTGVKEDDALRRSAAEI
                     VMTELHAGGKFDSNSYKVSGGLHGVGVSVVNALSEWLRLTIWREGQVHQIEFRNGVAV
                     APLKATGSTERRGTEVHFLASRETFGNVDYHYDILARRLRELSFLNNGVRIVLIDQRA
                     GKEENFAFSGGVKGFVEYMNRAKNVLHPNVFLGHGVKDGITVEVAMQWNDSYQENVQC
                     FTNNIPQKDGGTHLTGLRAAMTRVLNKYIEEHEIAKKAKVETTGDDMREGLACVLSVK
                     VPEPKFSSQTKEKLVSSEVRPVVEEIVAERLREFLDERPHDARTITVKIVEAAAAREA
                     ARKAREMTRRKGVLDSMGMPGKLADCQERDPAKSELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLKGKILNVERARIDKIITSTEVATLITALGAGIGEDFNLDKLRYHRIIIMTDADV
                     DGAHIRTLLLTFFYRQMPQLLERGHIYVAQPPLYKVKSGKDERYLKDEHELKQHQLRR
                     ALNGAQLNPGAGATPLAGEALERIASGYLTTEAVIERLSAAMDSAVLHAMLAMPRISL
                     SDKEGATQAAKEFYRVLSPNAPKSHIERDPTSDGWRL"
     gene            complement(1993..3099)
                     /gene="dnaN"
                     /locus_tag="VFX94_04005"
     CDS             complement(1993..3099)
                     /gene="dnaN"
                     /locus_tag="VFX94_04005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX5767378.1"
                     /translation="MNLARAKRDELLGPLSAVSGIIERRHTLPILSNVLIERGGDALA
                     FLATDIEIQITARSAVAAPSEARAVTVGARKLLDILRALPDNAEVTLSQQERRLLVKA
                     GKSRFSLQTLAAEDFPRLARPAGEAAKFELEQRALRRLLSLVQYAMAQQDIRYYLNGL
                     LMVVEDGSLKLVATDGHRLAYAALKLGAQLPRQEVIVPRKTVLELGKLLADSEAPVRV
                     EIAATQAAFSFGEVDLVSKLIDGKFPDYTRVIPASHKNRLQAAREPLRQALQRAAILS
                     NEKFRGVRWVLAEGSLKIVSSNAEQEEAQEELDVNYSGDALDIGFNVNYLLDVLNNVS
                     GEDIECAFGDAASSALVTYGSEKDFKYVVMPMRI"
     gene            complement(3293..4621)
                     /gene="dnaA"
                     /locus_tag="VFX94_04010"
     CDS             complement(3293..4621)
                     /gene="dnaA"
                     /locus_tag="VFX94_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX5767379.1"
                     /translation="MQNLWDACLRRLEQELPAQQFNTWIRPLAPERGEAANTLVLAAP
                     NRFVLELVRERFAGRIERLASEASGRELGLRLVLARVVEPAAQLSPAAPRVPETPSIL
                     ERARLNPAFSFDSFVTGKANQLARAAALQVAENPGVSYNPLFVYGGVGLGKTHLVQAI
                     GNQLADHRPQARIRYIHAEQYVTDVVRAYQQKSFDEFKRYYHSLDLLLIDDIQFFSNK
                     GRTQEEFFYAFNALVEAHKQIVITCDTYPKEIAGMEERLISRFGWGLTVAIEPPELEM
                     RVAIVLKKAEAESVALSEDIAFFIAKHLRSNVRELEGALKKVLAFARFSGRELSLELA
                     KEALTDILNVASRQVSVEGIQKTVAEYFKIKISDMHSKKRSRNVARPRQVAMALAKDL
                     TQMSLPEIGEAFGNRDHTTVLHACRTIASLRKHDSALNRDYVVLEQVLKG"
     gene            4740..4880
                     /gene="rpmH"
                     /locus_tag="VFX94_04015"
     CDS             4740..4880
                     /gene="rpmH"
                     /locus_tag="VFX94_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX5767380.1"
                     /translation="MATKRTYQPSKTRRARRHGFLVRSRTAGGRKVLRNRRARGRRRL
                     GL"
BASE COUNT          829 a         1645 c         1637 g          988 t
ORIGIN      
        1 caggcgccag ccgtccgagg tggggtcacg ctcgatatgg gacttgggcg cgttgggtga
       61 cagcacgcgg tagaactcct tggccgcctg cgtggcgccc tccttgtcgg agagcgagat
      121 gcgcggcatc gcgagcatcg cgtgcaggac cgccgaatcc atcgcggcgg acaggcgctc
      181 gatcacggcc tcggtggtga gatagcccga ggcgattcgt tccagcgcct cgccggcgag
      241 cggggtagcg cccgcgccgg ggttgagctg cgcgccgttg agcgcccggc gcagctggtg
      301 ctgcttcaac tcgtgctcgt ccttgaggta gcgctcgtcc ttgccgctct tcaccttgta
      361 gagcggcggc tgcgccacgt agatgtggcc gcgctcgagc agttgcggca tctggcggta
      421 gaagaaggtg agcagcagcg tgcggatgtg cgcgccgtcc acgtccgcgt cggtcatgat
      481 gatgatgcgg tggtagcgca gcttgtcgag gttgaagtcc tcgccgatgc cggcgcccag
      541 cgccgtgatc agcgtcgcca cctccgtcga tgtgatgatc ttgtcgatgc gcgcgcgctc
      601 gacgttgagg atcttgccct tgagcggcag gatcgcctgg aacttgcggt cgcggccctg
      661 cttggcggag ccgccggcgg aatctccctc cactagatag agctccgact tggccgggtc
      721 gcgttcttgg cagtcggcaa gcttgccggg catgcccatc gagtcgagca cgcccttgcg
      781 gcgcgtcatc tcgcgcgcct tgcgcgcggc ttcgcgcgcc gctgccgcct cgacgatctt
      841 taccgtgatg gtgcgcgcgt cgtgtgggcg ctcgtcgagg aattcgcgca gccgctcggc
      901 gacgatttcc tcgaccaccg gccgcacttc ggaggacacc agcttttcct tggtctgcga
      961 cgagaacttg ggctcgggca ccttgaccga gagcacgcac gcgagcccct cgcgcatgtc
     1021 atcgccggtg gtctccactt tggccttctt ggcgatctca tgctcctcga tgtacttgtt
     1081 cagcacgcgc gtcattgcgg cgcgcaggcc ggtgaggtgc gtgccgccgt ccttctgggg
     1141 aatattgttg gtgaagcact ggacgttttc ctggtaggag tcgttccact gcatcgccac
     1201 ctcgacggta atcccgtcct tcacgccatg gccgaggaac acgttcgggt gcagcacatt
     1261 cttcgcacgg ttcatgtact cgacgaaccc tttcactcct cccgagaagg cgaagttctc
     1321 ttccttgcct gcacgctggt cgatcaggac gatcctcacg ccgttgttga ggaacgaaag
     1381 ctcacgcagg cggcgtgcga ggatgtcata gtggtagtcg acgttgccga acgtctccct
     1441 cgaggcgagg aaatgcacct cggtgccgcg ccgctcggtg ctccccgtcg ccttgagcgg
     1501 cgccaccgcg acgccgttgc ggaattcgat ctggtgcacc tgtccttcgc gccagatcgt
     1561 aagccgcagc cactccgaca gggcgttcac caccgaaacc ccgacgccgt gcagcccgcc
     1621 agagaccttg tagctgttgg agtcgaactt gccgccggca tggagttcgg tcatcacgat
     1681 ctcggcggcc gagcgccgga gcgcgtcgtc ttccttgacg ccggtcggaa tgccgcggcc
     1741 gttatccagc acggacaccg aattatcggt gtgtatcgtg acgatgatct cgtcgcagaa
     1801 tcctgcgagg gcctcgtcga ccgcattgtc gactacctcg aacaccatgt gatgcaggcc
     1861 ggtgccatcc gaggtgtcgc cgatgtacat cccggggcgc ttgcgcaccg cctccaggcc
     1921 cttcaggacc ttgatcgcgt cggagccgta ctcgctctca ccgtttccct tgtgctcgga
     1981 catgcgctcg cttcagatcc tcatcggcat gacgacgtac ttgaagtcct tctccgagcc
     2041 gtaagtgacg agcgcgctgg aggccgcatc cccgaaggcg cactcgatgt cctcgcccga
     2101 cacgttgttc agcacgtcga gcaggtaatt cacgttgaaa ccgatgtcca gcgcgtcgcc
     2161 ggagtagttc acgtcgagct cctcctgcgc ttcttcctgc tcggcgttcg atgacacgat
     2221 cttcagcgag ccctccgcaa gcacccagcg cacgccccga aatttctcgt tcgacaggat
     2281 cgccgcgcgc tgcagcgcct gccgcaaggg ctcgcgcgcg gcttgcagac ggttcttgtg
     2341 gcttgccggg atcacccgcg tatagtccgg aaactttccg tcaatcagct tcgagacaag
     2401 gtccacctcg ccgaagctga acgccgcctg cgtcgccgcg atttccaccc tcacgggcgc
     2461 ttcgctgtcg gcgagcagct ttccgagttc aagcaccgtt ttgcgcggca cgatgacttc
     2521 ctgccgcgga agctgggcac cgagcttgag tgccgcgtag gcaagacgat gcccatccgt
     2581 ggcgacgagc ttcaggctgc cgtcctccac gaccatcagc aggccgttca agtagtagcg
     2641 gatgtcctgt tgcgccatgg cgtactgcac gagcgagagc agccgccgca gtgcgcgctg
     2701 ctcgagctcg aacttggccg cctcgccggc gggccgggcg agacgcggga agtcctctgc
     2761 ggcgagcgtc tggaggctga agcggctctt gcccgccttc accagcaggc gcctctcctg
     2821 ctggctgagc gtcacctcgg cgttgtcagg aagagcgcgc aggatgtcga gaagcttcct
     2881 tgccccgacg gttaccgcgc gggcctcgga cggggcggcg acggcgctgc gcgccgtgat
     2941 ctggatctcg atatccgtgg cgagaaaggc aagcgcatcg ccccctcgct cgatcagcac
     3001 gttggaaaga atcggcagcg tatggcggcg ctcgatgatc ccgctgacgg ccgaaagcgg
     3061 tccgagaagc tcgtcgcgct ttgctcttgc aagattcatt gttatttacc ttagtagtag
     3121 gtaatagagc gcccgcgatg gcgggataac cgggcaaccc gttgccgcgg ctgggtttac
     3181 gggcgcgccg gagccgtgca aaagctgtgg aaagggcgcg cggcaatgtg gattgttcgc
     3241 gcgccggcga caatcgagca ggttactcac aatccactcc caacttttgc cctcagcctt
     3301 tcagcacctg ctcgagcacc acatagtctc ggttgagcgc gctgtcgtgc ttgcgcagac
     3361 tcgcgatggt gcggcaggca tgcagcacgg tggtatggtc gcggttaccg aacgcctcgc
     3421 cgatctcggg caagctcatc tgcgtcaggt ccttcgcgag cgccatcgcg acctgacgcg
     3481 ggcgcgccac gttgcggctg cgcttcttgg aatgcatgtc ggaaatcttg atcttgaagt
     3541 actcggcgac cgttttctgg atgccctcga cgcttacctg acggctcgca acgttcagaa
     3601 tatcggtgag ggcttccttg gcgagctcaa gggaaagttc gcgccccgaa aagcgcgcaa
     3661 atgcgaggac tttcttcagt gcgccctcga gctcgcgcac gttcgagcgc aggtgcttgg
     3721 ctatgaaaaa agcgatgtcc tccgagagcg ctaccgactc ggcttcggcc ttcttcagca
     3781 cgatggcgac gcgcatctcg agctcgggag gctcgattgc gaccgtaagc ccccagccga
     3841 agcgcgaaat caaccgctcc tccatgccgg cgatctcctt cgggtaggtg tcgcaggtga
     3901 tcacgatctg cttgtgcgct tccaccagcg cattgaatgc gtagaagaat tcctcctggg
     3961 tgcggccctt gttcgagaaa aactggatgt cgtcgatcaa cagcaggtcg agcgagtggt
     4021 agtagcgctt gaactcgtcg aacgacttct gctggtacgc gcgcaccacg tcggtcacgt
     4081 attgctcggc atggatgtag cggatccggg cctgcgggcg atggtcggca agctgatttc
     4141 cgatcgcctg caccaggtgc gtcttgccga gtccaacgcc gccgtacaca aagagcgggt
     4201 tgtacgagac tcccgggttc tccgccacct gcagtgccgc ggcgcgcgca agctggtttg
     4261 ccttaccggt gacaaagctg tcgaacgaga acgcgggatt gagccgggcg cgctccagga
     4321 tcgaaggcgt ttcagggacg cgtggtgcag ccggcgagag ctgcgccgcc ggctcaacga
     4381 cgcgcgccag caccaatcgc aggccgagct cgcgcccgct cgcctccgag gcaagacgct
     4441 cgatgcgtcc ggcgaatcgc tcgcgcacga gctccaggac gaatcggttg ggcgctgcca
     4501 gcaccagcgt gttagccgcc tcgcccctct ccggcgcgag cgggcggatc caggtgttga
     4561 actgttgcgc ggggagctcc tgctcgaggc gccgcaggca agcatcccag aggttttgca
     4621 tcttttcgtt gggccggaag cgagtcggga ttttaccttc tcgggcatgc ctaatccaca
     4681 gacgaattga caatgttcgg acgcgcgcgc tctaataccg ccctttttcg gaccggcaga
     4741 tggcgaccaa gcggacctat caaccctcca agactcggcg cgcgcggcgc catggttttc
     4801 tcgtgcgcag ccgcactgcc ggtggtcgca aggtgctgcg caaccgccgc gcgcggggcc
     4861 ggcgccgact cgggctgtag gccgcggccg gcaagcgcct ggggctggat gctgcgcgcc
     4921 ggctgaggca aaagtcggat ttcgaacggc tgctgcgaaa cggcgcgcgg cgcaacctgg
     4981 acggctacac gatgtatgtg gcgcgacgcg atagcggatc cgcgcggctc ggcatgctga
     5041 tctcccgcaa gcacgcggcg cgcgcggtcg tacgcaatcg catcaagcgt tgtattcgc
//
LOCUS       DASUNT010000207         4118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_291376, whole genome shotgun sequence.
ACCESSION   DASUNT010000207 DASUNT010000000
VERSION     DASUNT010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291376"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VFX94_04020"
     CDS             complement(<1..905)
                     /locus_tag="VFX94_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5767381.1"
                     /translation="MNAPHCAGIETRAAVRATGLVKRYGELAAVDGLDLEIAPGEFLG
                     LLGPNGSGKTTTLHMLATLVRPSAGSASVAGFDVAADPVSVRAAIGLVFQDSALDRTL
                     TVAENLRFAGLLHDLPSRLIEERSRELLELFDLAERRDQPVAALSGGMRRALDIVRGV
                     LHQPRCLLLDEPTIGLDLPNRRRIWRFIERLRARTGMTVLLTTHYLEEADGCDRVVFI
                     RKGRLVESGAPGHLVARLGRHILEVESAELDGLVAVLEPTLGGSLREGDVAHFRCAAA
                     DLAALASLQAQVGARATAWRVRRPNL"
     gene            complement(902..2059)
                     /gene="pqqE"
                     /locus_tag="VFX94_04025"
     CDS             complement(902..2059)
                     /gene="pqqE"
                     /locus_tag="VFX94_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HEX5767382.1"
                     /translation="MPATRDGSVSARPNPPLWLLAELTYRCPLHCVYCSNPVNYTDYG
                     AELSTEDWVRVLREGRELGASQLGFSGGEPLMREDLETLAAEARRLGYYSNLITSGVG
                     LNERRIAALKSAGLDHIQVSFQDSTREMNDFLSSTRTFELKRRVARMVKQHGYPMVLN
                     VVLHRLNIEHVGQILELALELEADYVELANTQYYGFAYANRSELLPSREQLERAEEVV
                     ARFRERIGGRMKIYFVVPDYYASRPKACMNGWGAVFLCVTADGVALPCHEARMLPGLE
                     FPSVRSHALAWIWRDSPAFNRYRGDEWMREPCRSCPEKGKDYGGCRCQAYLLTGEAAN
                     ADPVCEKSPHHSLVTDATARARQKTSTTTARPVLLDRDDKTSRELSRKAPA"
     gene            complement(2019..2291)
                     /gene="pqqD"
                     /locus_tag="VFX94_04030"
     CDS             complement(2019..2291)
                     /gene="pqqD"
                     /locus_tag="VFX94_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HEX5767383.1"
                     /translation="MGEDTVFELARHYRFQWEPAQEAYVLLYPEGMVKLGASAGEILK
                     RVDGASAVSAIVADLERAFPGADLRGDVTEFLAHARDKGWIRVRPA"
     gene            complement(2284..3012)
                     /gene="pqqC"
                     /locus_tag="VFX94_04035"
     CDS             complement(2284..3012)
                     /gene="pqqC"
                     /locus_tag="VFX94_04035"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HEX5767384.1"
                     /translation="MNPAWSKREFEEKLRAKGARYHIHHPLHIAMNSGRCTKVQVQGW
                     VLNRFYYQISIPLKDAAILANMPEREHRRRWIRRILDHDGTEATHGGIEAWLRLGEAV
                     GLAREEVTSLRHVLPGVRFAVDAYVNFARRAPWQEAVCASLTELFAPEIHKERLAGWP
                     QHYPWIDPAGYAYFRGRLAEARRDVEHGLEVTLEHFRTREEQERALEILQFKLDVLWS
                     MLDAMQLAYGTGEAGIGEPGIGNG"
     gene            complement(3009..3920)
                     /gene="pqqB"
                     /locus_tag="VFX94_04040"
     CDS             complement(3009..3920)
                     /gene="pqqB"
                     /locus_tag="VFX94_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HEX5767385.1"
                     /translation="MRIRVLGSAAGGGFPQWNCNCRNCDGVRKGTIRAQPRTQSSIAV
                     SADREQWILFNASPDVLAQFRAFRELQPARALRDSAVRAIVLIDAQIDHTTGLLMLRE
                     GPRLQIYCTREVREDLIRGHPVMGILEHFCGVDWHPIAADPGTTFSVPGAEGLSFTAV
                     PLKSKPPPYSPHRNEPRPGDNIGVRIVDARTRRALFYAPGLGEIEPHLQPFLADADCV
                     MLDGTFWTDDELIRLGVSAKRARDMGHLPQSGRGGMLEALKPLAGRKILIHINNTNPI
                     LDEDSAERAALEAAGIEVAYDGMEIEL"
BASE COUNT          655 a         1373 c         1418 g          672 t
ORIGIN      
        1 aggttcgggc gcctgacgcg ccaggccgtc gcgcgcgcgc cgacctgcgc ctgcagactc
       61 gcgagggcag cgaggtcggc ggcggcgcaa cgaaaatgcg ccacgtcgcc ctcgcgcaag
      121 gacccgccca gcgttggctc cagcaccgcc acgaggccat cgagctcggc gctttcgacc
      181 tcgaggatat ggcggccgag ccgcgccacc agatgccccg gcgcgcccga ttccaccaga
      241 cggcccttgc ggatgaagac gacccggtcg cagccgtcgg cctcttcgag gtagtgcgtg
      301 gtgagcagca cggtcatccc ggtgcgcgcg cgcaggcgct cgatgaagcg ccagatgcgg
      361 cggcggttgg gcaggtccag gccgattgtc ggctcatcga gcagcaggca gcgcggctgg
      421 tgcaggacgc cgcgcacgat gtcgagcgcg cgccgcatgc cgcccgagag cgccgcgacc
      481 ggctgatcgc gccgctctgc gagatcgaag agctccagca gctcgcggct gcgctcctcg
      541 atcaaccgcg aaggcagatc gtggagcagg ccggcgaaac gcaggttttc cgccacggta
      601 agcgtgcggt cgagcgcaga gtcctggaac acgagaccga tggcggcgcg cacgctcacc
      661 gggtcggccg cgacgtcgaa gccggccacg gacgcggatc ccgcgctcgg ccggaccagc
      721 gtcgcgagca tatgcagcgt ggtggtcttg cccgagccgt tcgggcccag gaggccgaga
      781 aattcccccg gcgcgatttc gaggtccagg ccgtcgaccg cggccagctc gccgtagcgc
      841 ttgacgagcc ccgtagcgcg caccgcggcg cgcgtttcga tgccggcgca atgtggcgca
      901 ttcatgccgg ggcttttcgt gatagctcgc gggaggtctt gtcgtctcgg tcaaggagga
      961 cgggccgcgc cgtcgtggtg gaggttttct gacgagcgcg cgcggtcgcg tcggtgacca
     1021 gggaatgatg cggcgacttc tcgcagacgg ggtcggcatt ggccgcctcg ccggtgagga
     1081 gataggcctg gcagcggcag ccgccgtagt ccttgccttt ctccgggcag ctgcggcatg
     1141 gctcgcgcat ccattcgtcg ccgcggtaac ggttgaaggc gggcgaatcg cgccagatcc
     1201 aggcgagcgc gtggctgcgc acgctcggaa attccaggcc gggcagcatg cgcgcctcat
     1261 ggcagggcaa ggcgacgccg tccgcggtga cgcagaggaa cacggcgccc cacccgttca
     1321 tgcatgcctt gggccggctc gcatagtaat cgggaacgac gaagtagatc ttcatgcgcc
     1381 cgccgattcg ttcgcgaaat cgcgcgacga cctcttcggc gcgctcgagc tgctcgcggc
     1441 tcgggagaag ctcgctgcgg ttggcgtagg caaacccgta gtactgcgta ttggcgagct
     1501 cgacgtagtc ggcttcgagc tcgagcgcga gctccaggat ctgtccgacg tgctcgatgt
     1561 tcagccggtg cagcacgacg ttgagcacca tcggatagcc gtgctgcttc accatgcgcg
     1621 cgacccggcg cttcagctcg aacgtgcgcg tgctcgagag gaagtcgttc atctcgcggg
     1681 tcgagtcctg gaacgagacc tggatgtggt cgaggccggc gctcttgagc gccgcaatgc
     1741 gccgctcgtt cagcccgacg cccgaggtga tgagattcga gtaatagccc aggcgccgtg
     1801 cctcggccgc gagcgtctcc agatcctcgc gcatcagcgg ctcgccgccc gagaaaccga
     1861 gctggctggc gccgagctcg cgaccttcgc gcagtacccg cacccagtcc tcggtggaga
     1921 gctcggcgcc ataatcggtg tagttgaccg ggttggagca atacacgcag tgcagcgggc
     1981 agcggtacgt cagctccgcg agcagccaga gcggtgggtt aggccgggcg gacacggatc
     2041 catcccttgt cgcgggcatg cgcgagaaac tcggttacgt cgccgcgcag gtcggcgccg
     2101 ggaaaggcgc gctcgaggtc tgcgacgatg gccgacacgg ccgaggcacc gtcgacgcgc
     2161 ttgaggattt cccccgcgct tgcgccgagc ttcaccatgc cctcggggta gagcagcacg
     2221 taggcctcct gcgccggttc ccattggaaa cggtagtgcc gcgccagctc gaacacggtg
     2281 tcttcaccca ttgccgattc ctggctctcc aattcctgcc tctccagttc cataggcgag
     2341 ctgcatcgcg tcgagcatgc tccagagcac gtcgagcttg aattgcagga tctcgagcgc
     2401 gcgctcctgc tcctctcggg tccggaaatg ctccagcgtc acctccaggc cgtgctccac
     2461 gtcgcggcgc gcttcggcca gccggccccg aaagtaggcg tagcccgcag ggtcgatcca
     2521 gggatagtgc tgcggccagc ccgctaggcg ctccttgtga atctccggcg cgaagagctc
     2581 ggtgagcgat gcgcatacgg cttcctgcca gggagcgcgg cgcgcgaagt tgacgtacgc
     2641 atccactgcg aaacgcacgc ccggcagcac gtgacgcagc gaggtcacct cctcgcgcgc
     2701 gaggcccacc gcctcgccca gtcgcagcca tgcctcgatg ccgccgtggg ttgcttcggt
     2761 cccatcgtga tcgaggatgc gccggatcca gcgccggcga tgctcgcgct caggcatgtt
     2821 ggcgaggatc gcggcatcct tgagcggaat gctgatctgg tagtagaagc ggttcagcac
     2881 ccacccctgg acctgcacct tggtgcagcg gccgctgttc atggcgatgt gcaagggatg
     2941 gtggatgtgg tagcgcgccc ccttggcgcg gagcttctct tcaaactcgc gcttgctcca
     3001 cgccgggttc acagctcgat ctccattccg tcgtacgcga cctcgatccc ggcggcttcg
     3061 agcgcggcgc gttccgcgct gtcctcgtcc aggatcggat tggtgttgtt gatgtgaatc
     3121 aggattttgc ggcccgcgag gggcttcagc gcctcgagca tgccgccgcg cccgctctgg
     3181 ggcagatggc ccatgtcgcg cgcgcgcttc gccgaaacgc cgagccggat cagctcgtcg
     3241 tcggtccaga acgtgccgtc gagcatcacg cagtcggcat cggcgagaaa aggctgcagg
     3301 tgcggctcga tctcccccag tccgggcgcg tagaagagcg cgcgacgcgt gcgggcatcg
     3361 acgatccgca caccgatgtt gtcgcccggg cgcggctcgt tgcggtgcgg agagtacggc
     3421 ggcggcttgc tcttgagcgg cacggcagtg aacgacagcc cttcggcgcc cgggaccgag
     3481 aacgttgtgc ccggatcggc ggcgatcgga tgccagtcga cgccgcagaa gtgctcgaga
     3541 atgcccatca ccgggtggcc gcgaataagg tcttcacgca cctcgcgggt gcaatagatc
     3601 tggaggcgcg gaccctcgcg cagcatgagg agccccgtcg tgtggtcgat ctgggcatcg
     3661 atcaggacga ttgcgcgcac ggcgctgtcg cgcagcgcgc gcgcgggctg cagctcccgg
     3721 aacgcccgga actgcgcgag gacgtcgggc gaggcgttga acaagatcca ttgctcccgg
     3781 tccgccgaga ccgcgatcga cgattgtgtg cgcggctgag cgcgaatcgt gcccttgcgc
     3841 acgccgtcgc agttgcggca gttgcagttc cattgcggga acccgccccc cgcagcggat
     3901 ccgagcaccc ggatacgcat aaaaagcaag gcccctcgcg gggccctggt ctcggtaccg
     3961 gccgcggtca gcggctggcg atgtacatcg tcacctcgaa gccgaaacgc atctcggtgt
     4021 actcaggctt ggtccacatg tgtatctcct gattcggaag tgcaccgggc agcgcctagc
     4081 gccttccacg ccaattatgg tgcaaactgg ccgcgcca
//
LOCUS       DASUNT010000208         9143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_817305, whole genome shotgun sequence.
ACCESSION   DASUNT010000208 DASUNT010000000
VERSION     DASUNT010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9143
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817305"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VFX94_04045"
     CDS             complement(<1..371)
                     /locus_tag="VFX94_04045"
                     /inference="COORDINATES: protein motif:HMM:NF015791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEX5767386.1"
                     /translation="MHGPIYLTEDIRRIERGAGVAEAQLMERAAAAAAEVAARIASSA
                     SKDVLVLAGPGNNGGDARVVARILKDQFFRVELAAQMDQVPARSWGLVIDGLFGIGLA
                     REVGGEYAAMVDYANRQSCPVL"
     gene            940..1110
                     /locus_tag="VFX94_04050"
     CDS             940..1110
                     /locus_tag="VFX94_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767387.1"
                     /translation="MQKQPCAFAALASRRVISRPRGASITWPTAITPAPGGVWRSLRA
                     LRTKQRLVLRAR"
     gene            complement(1209..1379)
                     /locus_tag="VFX94_04055"
     CDS             complement(1209..1379)
                     /locus_tag="VFX94_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767388.1"
                     /translation="MKPAVLCAGDAGINALATVRALGRRGVPVDVVALESSRQVASAS
                     ARIQGACCRGRR"
     gene            complement(1438..2751)
                     /locus_tag="VFX94_04060"
     CDS             complement(1438..2751)
                     /locus_tag="VFX94_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX5767389.1"
                     /translation="MTPLAISVVLFASLLVLLVGGVWIAIAMGVVAWLGLALFTTSPP
                     EVNLFQAFWGSNASWTLAALPLFVWMGEILFRTRLSDEMFEGLAPWLGWLPGRLMHVN
                     VIGCGIFAAVSGSSSATCATIAKVALPELIRRGYDEKTCLGSLCGAGTLGLLIPPSII
                     MIVYAVAAEVSILKMFIAGVLPGIMLVLFFSGYIVVWALLNPEKTPRHVDHLSFRERL
                     HKARNLIPCVLLIAFVMGAMFTGYATATEAAAFGVLGALVIAWWSGSLTPANFWASLM
                     GATRLSCMILFILAGAAFLSSCMAFTGIPRALAQWVGSLGASPHALIAVLALIYLLLG
                     TALDGISMIVLTTSIVLPMIESAGFDLIWFGIFIVLLVEIAELTPPLGFNLFVLQTMS
                     GRDSNYVALASIPFFCMMVLSLVVVIAFPGIATWLPDALLGATRK"
     gene            complement(2748..3281)
                     /locus_tag="VFX94_04065"
     CDS             complement(2748..3281)
                     /locus_tag="VFX94_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX5767390.1"
                     /translation="MRRFLDRLYAASGALAAICLASIAVVMLAQALMRELGMLFRGAD
                     DMVAWLTAASAFLALGHTFRRGELVRIGLLIDRLPPRVQRPLALCALAVSVLFVGYMA
                     YAVLRFVFESWQFNELAQGLIQIPIWIPQMSFVLGALILFVAVVDEFLVLARGGKPAY
                     EAAEQERRARKDFSETL"
     gene            complement(3328..4290)
                     /locus_tag="VFX94_04070"
     CDS             complement(3328..4290)
                     /locus_tag="VFX94_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX5767391.1"
                     /translation="MIVRSILALLLVSGPAFAQTKWDMPTPYSDGEFHTRNVVAFVED
                     VKKATGGKLEIAVHSNGSLIKHPDVMRAVSTGQVNIAEFLLGQFGNDEPVFNADNVPF
                     LAAGYDAAWKLYEAQKPVLQKKLEGRGMRLLYCVAWPGQGIYTKEPLKSVGDLKGTKF
                     RTYSPMTARLAELLGASPTVVQVPEVPQMFATGAIHAMITSSATGTSTKAWEFVKNYY
                     TTSAMHPKNAVVVNQRGFARLPKDQQDALTKAAASAEKRGWDMSKAREKEGNETLAKN
                     GMSVREPDAAMKQAFATIGQQITQEWEKAAGADGQEILKRFRGK"
     gene            complement(4317..5411)
                     /locus_tag="VFX94_04075"
     CDS             complement(4317..5411)
                     /locus_tag="VFX94_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5767392.1"
                     /translation="MEKLDAVVIGAGVVGLAVARELARAGREVVILEAEDAIGTHTSS
                     RNSEVIHAGIYYPKGSLKARTCVAGKELLYEYCVAHGVPHRRSGKLIVSTDEKQTDEL
                     QKMQNRAHGNGVTDVVWMTRAQAQALEPEVWCTAALYSPATGIIDSHALMLAYLGDAE
                     DRGAMLALKSRFEKAVVKQLGFVVYAESADPIEARVLVNSAGLRAPSVARSIEGFDAK
                     LAPRELYAKGNYYTLTGRSPFSRLVYPVPEPGGLGVHVTLDLAGQARFGPDVEWVERI
                     DYQVDPQRAARFYAAIRRYWPGLRDGALAPGYAGIRPKTAGPGEPAPDFLLQGPRAHG
                     VPGLVNLFGIESPGLTASLALAAMVLEELR"
     gene            complement(5398..6582)
                     /locus_tag="VFX94_04080"
     CDS             complement(5398..6582)
                     /locus_tag="VFX94_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEX5767393.1"
                     /translation="MNAQQTPFRLVALLGVLVGLPALGTDLFVPALPLLAQALQVDAG
                     AAQFTLTTFFIGLAAGMLLWGPLSDRYGRKPVLVTGLAAMLGASLAAAHVDSVGAVAA
                     ARLVQGLAMSSGAVIARAMVRDLHAHEQAARLLASMTVVFSIIPIAAPLAGAAVAGLA
                     GWRAIFWSFSAIAMLLIAAAAAGLRETAPPERRSAHPAEILRTFRAILRESRFVAPFL
                     LVLSAVLGILAWVSSSAFVLVRGFGVSTTGYGFAFAAVMLGQITGAWASSQLVLRLGI
                     ARLVRVGAACMLAAGAIGASLAWLGIGHWAAVVVPFFLFLAGSALIVPNATAAALSPF
                     PGSAGAAASLMGAVSFTIGAMVSAGLGYAFDGTARPMATLSACAGILAFVLERKLFRG
                     KA"
     gene            6602..6730
                     /locus_tag="VFX94_04085"
     CDS             6602..6730
                     /locus_tag="VFX94_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767394.1"
                     /translation="MQVTLANWRTAPFNRWAFHHVRELVPLADIARAIAELRKQLA"
     gene            6723..>9143
                     /locus_tag="VFX94_04090"
     CDS             6723..>9143
                     /locus_tag="VFX94_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX5767395.1"
                     /translation="MPERWQFWIDRGGTFTDVVARRPDGALVTHKLLSENPGRYGDAA
                     LAAIREILSLKSSEAIPVELIECVKMGTTVATNALLERKGERTALFITRGFRDALRIA
                     YQNRPRIFDRHIVLPEMLYSKVVEVQERVGARGEVVSPLDSLRLATDLRDTFSEGYRS
                     VAIVFMHGYRFPGHERKAAELARAEGFTQVSVSHEVSPLMKLVGRGDTTVVDAYLSPI
                     LRRYVQELARELGGVRLLFMQSNGGLVDARRFQGKDAILSGPAGGIVGAVRTSAAAGF
                     DRIIGFDMGGTSTDVSHFAGEFEREFETRVAGVRMRAPMMSIHTVAAGGGSILHFDGA
                     RYRVGPDSAGANPGPACYRRDGPLTVTDANVMLGKIQPGYFPHVFGPQGDQPIDVAAV
                     RSAFRELQTGIADGRSAEQVAEGFVQIAVGNMANAIKHISVQRGHDVTRYTLCCFGGA
                     AGQHACLVADALAMKRIFIHPHAGVLSAYGMGLADQVAMRSHAVEARLGGADLDGIAA
                     VLAIEARNELLAQGIAADRIQIERKAHLKYEGTDTALIIALGPVPAMERDFEAAYRKQ
                     FSFLMPGRALVAEALSVEAIAKGSAFEEHELPARRDAKPSETVRMFTGGRWREAPLYR
                     RDDLGPGSRVDGPAIIAEANATTVVEPEWRASVTPLNHLVLERIRERATSSAIGTRVD
                     PVMLEIFNNLYMSIAEQMGLRLQNTAYSVNIKERLDFSCALFDAEGNLIANAPHMPVH
                     LGSMGESVKTVIRLNAGKMRPGDVYVLNAPYNGGTHLPDVTVITPVFENNDILFYVGS
                     RGHHADIGG"
BASE COUNT         1606 a         3052 c         3129 g         1356 t
ORIGIN      
        1 agcaccgggc agctctgccg gttcgcgtag tccaccatcg cggcgtactc gccgccgacc
       61 tcgcgcgcga gcccgatgcc gaaaagcccg tcgatcacga gcccccatga ccgcgcggga
      121 acctggtcca tctgcgcggc gagctctacg cggaagaatt ggtccttcag gatcctcgcc
      181 acgacccggg cgtcgccgcc gttatttccg gggccggcga gcacgaggac gtccttgctc
      241 gcgctagatg cgatcctcgc cgccacttcg gccgccgcgg cggcggcgcg ctccatcagc
      301 tgcgcctcgg cgactccggc accgcgctcg atgcgccgga tgtcctccgt gaggtagatc
      361 ggaccatgca cgcgcccgat tctaaatgaa cgtcgatcga caaaattgcc gcacgtatga
      421 ccgcattcac ccccgggcgg catgctgcga gactcgaacg ccagctgcgc tggctcaagc
      481 gccgcttcca cagcgttccg ctgcgcgcgc gcgagttcgg cgcgccggcc gatatcgcgg
      541 ggttcgtgtt gtggatgaag cgccttgagc tcgcggcgcg cacgcggctc gaagcccggc
      601 tggccgcggc gacgccgcgc ttcgagccaa cgcccgccga gcggaggaat tcgatcgggc
      661 gagccggccg agcgacttgc gccagggcgc gagatcgtcg agcgcgaaga gcgcccactc
      721 ggcgcgtccg aggtaggggc gccaagccgc gagcggacgg cgcggcgcct tcgataggtt
      781 ctgcatcggc ctgcaggctg atccagtaat gctcgccgga gaactcggac ccgattgcgg
      841 ccaggcgcgc cggctcggtg tggtcgcagt aggcgagcca ggcgaggtcg aagcttgcgc
      901 gccagccgat cgactggaag tgcgccgggc gcgggttcgg tgcaaaagca gccgtgcgcg
      961 tttgccgcgt tggcgagccg ccgcgtcatc tcgcgtcccc gcggcgcatc gatcacctgg
     1021 ccgactgcca tcacgccggc gcccggcggc gtctggcgca gcttgcgcgc gctgaggacg
     1081 aagcagcgcc tggtgctgcg cgcgcggtag cggagcgcta cgtggatgtg gagtcgtccc
     1141 cctcgacgta ctcctgcacc aagacctcgg cggggaaggc aatgcgcccg cgcagcgctg
     1201 cgccgaggct agcgccgtcc gcggcaacaa gcgccttgaa tccgcgcgct cgcggacgcg
     1261 acctggcggc tggactcgag ggcgaccacg tccaccggga cgccgcgccg gccgagcgca
     1321 cgcacggtgg cgagcgcgtt gattccggcg tccccggcgc acaacacggc gggcttcatc
     1381 ggtacaaatt tagcgcgcgc gcagcatggc aggcgacgcc gatctgcgcc tgtcggacta
     1441 ttttcgcgtc gcgccgagca gcgcgtcggg cagccaggtg gcgatccccg ggaatgcgat
     1501 caccacgacg agcgagagca ccatcatgca gaaaaacgga atggaagcga gcgcgacata
     1561 gttggagtcg cgcccgctca tggtctgcag cacgaaaaga ttgaagccga gcgggggggt
     1621 gagctccgcg atctcaacga gcagcacgat gaagatgccg aaccatatga gatcgaagcc
     1681 cgccgactcg atcatcggca gcacgatcga ggtcgtcagc acgatcatgg agatgccgtc
     1741 gagcgcggtg ccgagcagaa ggtagatcag cgccagcaca gcgatgagcg catggggact
     1801 tgcgccgagc gagcctaccc actgcgcgag cgcccgcggg atgccggtaa aggccatgca
     1861 ggaactgagg aacgccgcgc cggcaaggat gaagaggatc atgcacgaca ggcgcgtcgc
     1921 gcccatcagg ctcgcccaga agttcgcagg cgtaagcgag ccgctccacc acgcgataac
     1981 gagcgcgccg agcacaccga acgccgccgc ctcggtggcc gtcgcgtacc cggtgaacat
     2041 tgcgcccatc acgaacgcga tgagcagcac gcaggggatc aaattgcgcg ccttgtgcag
     2101 cctctcccgg aaagaaaggt ggtcgacgtg ccgcggcgtc ttctccgggt tgagcagcgc
     2161 ccacaccacg atgtagccgg agaagaagag cacgagcatg atgccgggaa gcaccccggc
     2221 gatgaacatt ttcaggatcg aaacctcggc ggccaccgcg tacacaatca tgatgatcga
     2281 cggcgggatg agcaggccga gcgtgccggc gccgcacagg gagcccaggc acgtcttctc
     2341 gtcgtacccg cggcggatga gctccggcag cgcgactttc gcgatcgtcg cgcaagtggc
     2401 ggaggaggac ccggacaccg cggcgaagat gccgcagccg atgacgttga cgtgcatcag
     2461 gcgcccgggc agccatccca gccacggcgc gaggccctcg aacatctcgt cggagagccg
     2521 ggtgcggaac aggatctcgc ccatccagac gaaaagcggc agcgcggcga gcgtccagga
     2581 cgcgttggat ccccagaacg cctggaacag gttgacctcc ggcgggctcg tggtgaaaag
     2641 cgccagcccc agccatgcca ccacgcccat ggcgatcgca atccacaccc cgccgacgag
     2701 cagcacgagg aggctggcga acaggacgac cgaaatggcg agaggcgtca cagcgtttcg
     2761 gagaaatcct tgcgcgcgcg gcgctcttgc tcggcggctt cataagccgg cttgccgcct
     2821 cgcgccagca ccaagaactc atcgaccacg gcgacgaaga ggatcagcgc gccaagcacg
     2881 aagctcatct gcgggatcca gatcgggatc tggatgaggc cctgcgcaag ctcgttgaac
     2941 tgccagctct cgaacacgaa gcgcaagacg gcgtaggcca tgtaacccac gaagagcacg
     3001 ctgaccgcga gcgcgcaaag cgcgagcggg cgttgcaccc gcggcggcag ccggtcgatg
     3061 agcaggccga tgcgtacgag ctcaccgcgc cggaaggtgt ggccgagcgc gaggaacgcg
     3121 ctcgctgcgg tgagccaggc gaccatgtcg tcggcaccgc ggaacagcat gccgagctcg
     3181 cgcatgagcg cctgcgcgag catgacaacg gcgatcgacg cgaggcagat cgccgcgagc
     3241 gcgccgctcg cggcgtagag gcggtcgagg aaccgcctca tccgaaaagg gagcgcgggg
     3301 gtaaggcgat tatcaccccc gcttccccta cttgccgcgg aagcgcttga gaatttcctg
     3361 gccgtcggcg ccggcggcct tttcccattc ctgcgtgatc tgctggccga tcgtggcgaa
     3421 cgcctgcttc atggccgcat cgggctcgcg cacgctcatg ccgtttttcg ccagcgtctc
     3481 gttgccttcc ttctcgcgtg ccttcgacat atcccagccg cgcttttcgg cgctggcggc
     3541 cgccttcgtc agcgcgtcct gctggtcctt cggcagccgg gcaaagccgc gctggttcac
     3601 caccaccgcg ttcttcgggt gcatggcgct cgtcgtgtag tagttcttga cgaattccca
     3661 ggccttggtc gaagtgccgg tggccgagga ggtgatcatg gcgtggatcg cgccggtcgc
     3721 gaacatctgt gggacttcgg gaacctgcac cacggtcggg ctcgcgccga ggagctcggc
     3781 gaggcgcgcg gtcatcgggc tgtaggtgcg gaacttggtg cccttcaggt cgccgaccga
     3841 cttgagtggc tccttggtat agatgccctg gccgggccac gcgacgcagt agagcagccg
     3901 catgccgcga ccttcgagct tcttctgcag caccggcttc tgcgcctcgt agagcttcca
     3961 cgccgcgtcg tagccggcgg cgaggaacgg cacgttgtcg gcgttgaaca ccggctcgtc
     4021 gttgccgaac tgcccgagca ggaattcggc gatgttgacc tgcccggtcg agaccgcgcg
     4081 catgacgtcc gggtgcttga tgagcgagcc attggaatgc accgcgatct cgagcttgcc
     4141 accggttgcc tttttcacgt cttcgacgaa ggccaccacg ttgcgcgtat ggaactcgcc
     4201 gtcggagtat ggcgtcggca tgtcccactt ggtctgcgcg aacgcgggac ccgacaccag
     4261 caataatgca agtatgctgc gaacgatcat ggactcctcc tcaaggacgc tgaatcttag
     4321 cgtagttcct ccagcaccat cgcggcgagc gccaggctcg cggtcagacc gggcgactcg
     4381 atgccgaaga ggttcacgag cccgggaacg ccgtgtgcgc gcgggccctg tagcaggaag
     4441 tcgggcgcgg gctcgcccgg gcccgcggtc ttcggccgga taccggcgta tccgggagcg
     4501 agtgcgccgt cgcgcaggcc gggccagtac cggcggatgg cagcatagaa ccgcgctgcg
     4561 cgctgcgggt caacttggta gtcgatgcgc tccacccact cgacatcggg cccgaagcgc
     4621 gcctgcccgg cgaggtccag cgtgacgtgc acgcccaggc cgcccggctc gggcacgggg
     4681 tacaccaggc gcgaaaacgg cgagcggccg gtcagggtgt agtaattgcc cttggcgtag
     4741 agctcgcgcg gtgcgagctt cgcgtcgaag ccttcgatgg agcgcgcgac gctcggcgcg
     4801 cgcagaccgg cgctgttcac gaggacccgg gcctcgatgg ggtctgcgct ttcggcgtag
     4861 accacgaagc cgagctgctt caccaccgct ttttcgaacc ggctcttgag cgccagcatc
     4921 gcgccgcgat cctcggcgtc gcccagatag gcaagcatga gggcgtggct gtcgatgatg
     4981 ccggtggcag gcgaataaag cgccgccgtg caccaaacct cgggctcgag cgcctgcgcc
     5041 tgcgcgcgcg tcatccacac gacgtcggtc acaccgttgc catgcgcgcg gttttgcatt
     5101 ttctgaagtt catcggtttg cttctcatcg gtggagacaa tcagcttccc gctgcggcga
     5161 tgcggcacgc cgtgggcgac gcagtactcg taaaggagct ccttgccggc gacgcaggtg
     5221 cgcgccttga gcgagccctt cgggtagtag atgccggcgt ggatcacttc cgagttgcgc
     5281 gagctggtat gggtaccgat cgcgtcctcg gcctcgagga tcacgacttc gcgcccggcg
     5341 cgcgccagct cgcgcgcgac agcgaggccg accaccccgg caccgatcac gaccgcgtca
     5401 agcttttcca cggaagagct tcctctcgag aacgaacgca aggatcccgg cgcaggcgct
     5461 gagcgtggcc atcggccgcg cggtgccatc gaaggcgtag ccgaggcccg cgctcaccat
     5521 cgcgccgatc gtaaaggaca ccgcgcccat cagcgaagcc gccgcgccgg cggagccggg
     5581 aaatggcgag agcgcggccg cggtcgcatt cggaacgatg agcgcgctgc cggcgaggaa
     5641 aaggaagaag ggcaccacca ccgccgccca gtgaccgatg ccgagccacg cgagcgaggc
     5701 gccgatcgcc ccggccgcca gcatgcaagc ggcgccgacg cgcacgaggc gtgcaatgcc
     5761 gaggcgtagc acgagctggc tggaagccca cgcgccggtg atctggccca gcatgacggc
     5821 ggcaaacgcg aacccgtagc ccgtcgtcga gacgccgaac ccccgcacca gtacgaatgc
     5881 cgagctcgac acccaggcga ggatgccgag aaccgcgctc agcacgagaa ggaacggggc
     5941 gacgaaccgg ctctcgcgca ggatggcgcg aaacgtgcgc aggatttcgg cgggatgcgc
     6001 ggatcggcgc tcgggcgggg cggtctcgcg cagtccggcg gccgcggcgg cgatgagcag
     6061 catggcgatc gcgctgaagc tccagaaaat agcgcgccag ccggccagtc ctgcaaccgc
     6121 ggccccggcg agcggcgcgg cgatgggaat gatgctgaat acaaccgtca tgctggcgag
     6181 aaggcgcgct gcctgctcat gcgcgtgcaa gtcgcgcacc atggcgcggg caatgacggc
     6241 gccgctcgac atggcgagcc cttggacaag ccgcgccgcg gcgaccgcgc cgaccgaatc
     6301 gacatgggcg gcggcgagcg aggcaccgag catggcggcg aggccggtca cgaggacggg
     6361 ctttcgtccg tagcggtccg agagcggtcc ccagagaagc attccggcgg cgaggccgat
     6421 gaagaaggtc gtgagcgtga attgcgccgc gccggcgtca acctgcagcg cctgcgcaag
     6481 cagcggcaac gccggcacga aaagatccgt ccccagcgcc ggcagcccga ccagcacgcc
     6541 gagaagcgcg acgaggcgaa acggggtctg ctgcgcgttc aagttaggat gcgcattcta
     6601 aatgcaggtc acgctcgcca actggcgcac cgcgccgttc aaccgatggg ccttccacca
     6661 cgtgcgcgag ctcgtgccct tggccgacat tgcgcgcgcc atcgccgagc tgaggaagca
     6721 gcttgcctga gcgctggcag ttctggatcg accgcggcgg cacgtttacc gacgtggtgg
     6781 cgcgcaggcc cgatggcgcg cttgtgaccc acaagctgct ttccgagaac ccggggcgct
     6841 acggcgacgc ggcgctcgcc gcgatccggg aaatactgtc actcaagagc tccgaagcga
     6901 taccggtcga gctcatcgaa tgcgtgaaga tgggcacgac ggtggcgacc aacgcgcttc
     6961 tcgagcgcaa gggcgagcgc accgcgcttt tcattacccg cggcttccgc gacgccctgc
     7021 gcatcgcgta ccagaaccgc ccacgcatct tcgaccggca catcgtgctg ccggaaatgc
     7081 tctactcgaa ggtcgtggag gtgcaggagc gcgtcggcgc ccggggagag gtcgtttcac
     7141 cgctggacag ccttcgcctg gcgaccgatt tgcgagacac gttttccgag ggctatcgct
     7201 cggtcgccat cgtcttcatg cacggatacc gtttccccgg gcacgagcgc aaggcggcgg
     7261 agctcgcgcg cgccgaaggc ttcacccagg tctcggtgtc gcacgaagtg agtccgctca
     7321 tgaagctcgt gggccgcggg gatacgacgg tagtggacgc ctatctctca ccaatcctgc
     7381 gccgctacgt gcaagagctt gcgcgggagc tgggcggggt gcggctgctc ttcatgcagt
     7441 cgaacggcgg gctggtggac gcgcgccgtt ttcagggcaa ggacgcgatc ctttccgggc
     7501 ctgcgggggg catcgtgggc gcagtgcgga cctccgcggc ggcgggcttc gatcgtatca
     7561 tcggcttcga catgggcggg acgtcgaccg acgtttcgca cttcgccggc gaattcgagc
     7621 gcgagttcga gacgcgcgtc gccggggttc gcatgcgcgc gcccatgatg agcatccaca
     7681 ccgttgcggc gggcggcggc tcgatcctgc acttcgacgg cgcgcgctac cgcgtcggcc
     7741 cggattccgc cggcgcgaat cccgggccgg cgtgctaccg gcgagacggc ccgctcaccg
     7801 tcaccgacgc gaacgtgatg ctcggcaaga tccagccggg ctacttcccg cacgtcttcg
     7861 ggccgcaagg cgaccagccg atcgacgtcg cggcggtgcg aagcgcgttc cgagaattgc
     7921 aaacaggcat cgcggacggt cgaagtgccg agcaggtggc cgagggcttc gtgcaaattg
     7981 ccgtcggcaa catggccaat gccatcaagc acatctccgt gcagcgcggc cacgacgtca
     8041 cgcgatatac gctctgctgc ttcggcggcg ctgccggtca gcatgcctgc ctcgtggccg
     8101 atgcgctcgc catgaagcgc atcttcatcc atccgcacgc gggcgtgctc tcggcgtacg
     8161 gcatggggct tgccgaccag gtcgcgatgc gcagccacgc ggtcgaggcg cggctcggcg
     8221 gcgccgatct cgacggcatc gccgcagtgc tcgccatcga ggcacgcaac gagctcctgg
     8281 cgcaggggat agcggccgat cgcatccaga tcgaacgcaa ggcgcacctg aagtacgagg
     8341 gcaccgacac cgcgctcatc atcgcgctcg gtccggtgcc cgccatggag cgcgacttcg
     8401 aggccgctta tcgcaagcaa ttctcgtttc tcatgcccgg ccgggcgctc gtggccgaag
     8461 cgctatcggt ggaggcgatt gcgaagggtt cggctttcga agaacacgaa ttgcctgcgc
     8521 gacgggatgc gaagccctcc gaaaccgtac gcatgttcac cggcggacgc tggcgcgagg
     8581 cgccgctcta ccgccgcgac gacctcgggc cgggcagccg ggtcgacggc ccggcgatta
     8641 tcgccgaggc gaacgccacg accgtggtgg agcccgagtg gcgcgcgagc gtcactcccc
     8701 tgaaccacct ggtgctggaa cgtatccggg agagagcgac aagcagcgcg atcggtaccc
     8761 gggtcgaccc ggtgatgctc gagattttca acaatctcta catgtccatc gccgagcaga
     8821 tggggcttcg cctgcagaac accgcctact cggtgaacat caaggagcgg cttgacttct
     8881 cgtgcgcgct tttcgacgcc gaaggcaatc tgatcgcgaa cgccccgcac atgccggtgc
     8941 acctgggctc gatgggcgag tcggtgaaga cggtgatccg gctgaacgcc gggaagatga
     9001 ggccgggaga tgtgtacgtg ctcaacgccc cctacaacgg cggcacgcac ctgccggatg
     9061 tgacggttat tactccagtc tttgagaaca acgacatcct tttctacgtc ggttcgcgcg
     9121 ggcatcacgc cgacatcggc ggc
//
LOCUS       DASUNT010000209         3652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1256400, whole genome shotgun sequence.
ACCESSION   DASUNT010000209 DASUNT010000000
VERSION     DASUNT010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256400"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VFX94_04095"
     CDS             complement(<1..393)
                     /locus_tag="VFX94_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767396.1"
                     /translation="MIATKAQKVGNGWARAYVALDASGNALALGVSLDKDALAGLPKE
                     PHPASRCFDKNGNGKMDAHECLGDYAYIFELPEGEAARAVAPFKWVSLNWNPHGHVPP
                     APPPWSAPHFDFHFYLQDREAVKQIRTGT"
     gene            533..1525
                     /gene="mltG"
                     /locus_tag="VFX94_04100"
     CDS             533..1525
                     /gene="mltG"
                     /locus_tag="VFX94_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX5767397.1"
                     /translation="MLKILLGLVAVAFAAAAAYAAWYLGSPVAIGRYPVEVEIPRGAG
                     FRTAMEQLEKSGIAMRRYEFELLARALRKERDIKAGSYEISQPVTPLELLQRLTRGDV
                     TQAEVRLLEGWTFAQFRAALDSSPHLRHHTKDMTDADILTRLNIAETHPEGLFFPDTY
                     LFGKGASDLAVLRRSYRAMARRLKEEWDAKDPAVPYRSPYEALIMASIIEKETGQAGE
                     RDMIGGVLVNRLRIGMRLQVDPTIIYGLGAAFDGNLKRGHLAEDGPYNTYTRAGLPPT
                     PISMPGLASLRAAVRPARTDAMYYVSRNDGSSHFSRTLEEHNRAVARYQLRGGR"
     gene            1522..2130
                     /gene="tmk"
                     /locus_tag="VFX94_04105"
     CDS             1522..2130
                     /gene="tmk"
                     /locus_tag="VFX94_04105"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX5767398.1"
                     /translation="MSARGKFVTLEGMDGAGKSTHQKFVADFVAMRAPHVIQTREPGG
                     TDLAERLREAILSQSMAPEVETLLIFAARADHVARVIRPALEAGTWVVCDRFTDATAA
                     YQGAGKGVPGDLIERLAAAAHPGLVPDRTLVFDCSFEVSRQRLQGRTLDRFEREDRAF
                     FERVRAAYLERSRAESQRVRLIDASVPVPEIRKALERELADL"
     gene            2130..3020
                     /gene="holB"
                     /locus_tag="VFX94_04110"
     CDS             2130..3020
                     /gene="holB"
                     /locus_tag="VFX94_04110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX5767399.1"
                     /translation="MHPWNQPILDSLARRLERLPHALLFHGPRGVGKLALAEKASQLL
                     LCEGAPRRPCGACDACRWFQAGNHPDFRRVEPEILWAEKPEKASLVLKVEQVRELAGF
                     LYTGSHRGRLRVALVHPAEDMNENAANALLKGLEEPPADAVFILVSHRPAGLLPTIRS
                     RCVAVPVPLPPREAAFEWLAGQGVKDAQRWLAYAGGAPLRALQHAARGPVTLAPVGER
                     DELEPLADALQKIAVDRALAAFGLPAKYQTSGASVSPQKARAWLAFARRMGEERLLCR
                     HPLNARLFSSSMLAAAPDLT"
     gene            complement(3098..3562)
                     /locus_tag="VFX94_04115"
     CDS             complement(3098..3562)
                     /locus_tag="VFX94_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEX5767400.1"
                     /translation="MIVFDLLCAAGHRFEGWFGSVRDFASQRDRNLLACPACGSAKVS
                     KVPSATRANLGAPEPKPAPKADKAPEKTPEMEGKDPFAIAQMLYSRMLDELLSKSEDV
                     GREFPSEARRIYYEEAPARAIRGEATQQEHQELVDEGIPVARLPVPPSGKLN"
BASE COUNT          572 a         1268 c         1194 g          618 t
ORIGIN      
        1 ggtgccggtg cggatctgct taaccgcctc gcggtcctgg aggtagaaat ggaaatcgaa
       61 atgcggcgcg gaccagggcg gcggagcggg ggggacgtgg ccgtgcggat tccagttcag
      121 gctgacccac ttgaacggcg caaccgccct ggctgcttcg ccctccggca gctcgaagat
      181 gtaggcgtag tccccgagac attcatgcgc gtccatcttc ccgttgccgt tcttgtcgaa
      241 gcagcggctc gcgggatgcg gctccttcgg cagccctgcg agggcgtcct tgtccaggct
      301 cacgcccagc gcgagcgcgt ttccgctcgc atcgagcgcc acgtaagccc gggcccatcc
      361 gttgccgacc ttctgcgcct ttgtggcgat cactctaggt tccgcggccg cggccaggct
      421 tgcagcgcac agtaccggca aaacgacgac agcaactaat ccccgtccga gctttttcat
      481 cgtagcctcc gcgtagaatg cgaattttag cgccgcgttc ttccccaggt tcttgctaaa
      541 gattcttctc ggcttagtgg ctgtcgcctt cgccgccgcc gcggcctacg cagcgtggta
      601 cctcggctcg ccggtcgcga tcggcagata cccggtggag gtggagattc cgcgcggcgc
      661 aggctttcgc accgcgatgg agcagctcga gaagtccggc atcgccatgc ggcgctacga
      721 gttcgagctt ctcgcgcggg cgctccgcaa ggaacgcgac atcaaggcgg gcagctacga
      781 gatatcgcag ccggtgacgc cgctcgagct gctgcagagg ctcacgcgcg gcgatgtcac
      841 ccaggccgag gtgcggctgc tcgagggatg gacattcgcc cagttccgcg ccgcgctcga
      901 ctcgagcccg cacctgcgcc accacaccaa ggatatgacc gatgccgata tcctgacgcg
      961 gctgaatatc gccgagaccc atcccgaagg gcttttcttt cccgatacgt atcttttcgg
     1021 caagggcgcg agcgatctcg ccgtgctgcg ccggagctac cgcgccatgg cgcggcgcct
     1081 gaaggaagag tgggacgcga aagatcccgc cgtaccgtac cgctcgccct acgaagcact
     1141 catcatggcg tcgatcatcg agaaggagac gggccaggcc ggcgagcgcg acatgatcgg
     1201 cggcgtgctg gtgaaccgcc tgcgcatcgg catgcggctg caggtggacc cgacgatcat
     1261 ctacgggctc ggcgccgcct tcgacggcaa cctcaagcgc ggccacctgg ccgaggacgg
     1321 tccctacaac acctacacgc gcgccgggct tccgcccacg ccgatcagca tgccggggct
     1381 cgcctcgctg cgcgccgcgg tgcgcccggc aaggaccgac gccatgtact acgtttcgcg
     1441 caacgacggt tcgagccact tctcgcgcac gctcgaggag cacaaccgcg ccgtggcgcg
     1501 ctaccagctg cgcggcgggc gatgagcgcg cgcggcaagt tcgttacgct tgaaggcatg
     1561 gacggagcgg gcaagtccac gcaccagaaa ttcgtcgccg atttcgtcgc gatgcgcgcg
     1621 ccgcacgtca tccagacgcg cgagccgggg ggcacggacc tcgccgagcg gctgcgcgag
     1681 gcgattcttt cccagtccat ggcgcccgag gtggagacct tgctcatctt cgccgcgcgc
     1741 gccgaccatg tcgcgcgcgt gatccggccg gcgctcgaag ccgggacctg ggtggtgtgc
     1801 gaccgcttta ccgacgccac cgctgcatac cagggagcgg ggaagggcgt gcccggcgat
     1861 ctgatcgagc gtctcgcggc ggcggcgcac ccgggactgg tcccggaccg cacgctcgtc
     1921 ttcgactgct cgttcgaggt gtcgcgccag cggctgcagg gaaggacgct cgatcgcttc
     1981 gagcgcgagg accgcgcgtt cttcgagcgc gtgcgagctg cctacctcga gcgctcgcgc
     2041 gcggagtcgc agcgcgtgcg actgatcgac gcgtccgtgc cggtgcccga gatccgcaag
     2101 gcgctcgagc gcgagctcgc cgacctctga tgcacccctg gaaccagccg atcctcgatt
     2161 cgctcgcgcg ccgcctcgag cgcctgccac acgcgctgct cttccacggg ccgcgcggcg
     2221 tggggaagct cgcgctcgcc gaaaaggctt cccagcttct tctttgcgag ggagcgccaa
     2281 ggcggccctg cggcgcctgc gatgcctgcc gctggttcca ggcgggcaac catcccgatt
     2341 tccgccgcgt cgagcccgaa attctctggg ccgaaaagcc cgaaaaggcg agcctcgtcc
     2401 tcaaggtaga gcaggtgcgc gagctcgccg gctttctgta caccggctcg catcgcggcc
     2461 gcctgcgggt ggcgctcgtg cacccggccg aggacatgaa cgaaaacgcc gccaatgcgc
     2521 tcctcaaggg cctcgaggag ccgcccgccg acgcggtctt catcctggtg tcccaccgtc
     2581 cggccgggct tctgccgacc atccgcagcc gctgcgttgc cgtgccggtt ccgctgcccc
     2641 cgcgcgaagc cgctttcgag tggctcgccg ggcagggcgt caaggatgcg cagcgctggc
     2701 tggcctacgc cggcggcgcg ccgctgcgcg cgctccagca cgccgctcgc ggtcccgtca
     2761 cgctcgctcc ggtcggagag cgcgacgagc tggagccgct cgccgacgcg cttcagaaaa
     2821 tagcggtcga ccgcgcactc gcggccttcg gcctgccggc gaagtaccag acctcgggcg
     2881 ccagcgtgtc accgcagaaa gcgagagcct ggctcgcttt tgcccgcagg atgggcgagg
     2941 agaggcttct ttgccgtcat ccgctgaacg ctcggctctt ttcctcgtcc atgctggccg
     3001 ccgcgcctga tttaacataa tacacattgc ctgaaatagt actgcaggaa gacaattagc
     3061 ttgcacggct aactgcctgg cctacaggtg tatttggcta gttgagcttg ccgcttggcg
     3121 gcacgggcag cctcgcgacc ggaatgcctt cgtccacgag ttcctgatgc tcctgctgtg
     3181 ttgcctctcc ccggatagca cgcgccggcg cctcttcgta gtagatgcgg cgcgcttcgg
     3241 aaggaaactc gcggcctacg tcttcactct tggaaagcag ctcgtccagc atgcgcgaat
     3301 agagcatctg cgcgattgcg aacgggtcct tgccctccat ctccggggtt ttttctggcg
     3361 ccttgtccgc cttgggagcc ggcttcggct cgggcgctcc cagattggcg cgggtcgccg
     3421 acggaacctt ggagaccttg gccgagccgc aggccgggca ggcaaggagg tttcgatcgc
     3481 gctgcgaggc gaagtcccgg accgagccga accacccctc gaaccggtgg cccgccgcgc
     3541 agagcaagtc gaacacgatc attccatgga ttctaccctt aggctcggcg ctttcccgcc
     3601 cggctgagcg gatcgacgcc cgcataggcg agaaagtcga tcgcgcggaa ct
//
LOCUS       DASUNT010000210         2607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_203877, whole genome shotgun sequence.
ACCESSION   DASUNT010000210 DASUNT010000000
VERSION     DASUNT010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203877"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VFX94_04120"
     CDS             complement(<1..647)
                     /locus_tag="VFX94_04120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5767401.1"
                     /translation="MEWIESYAAELEAIGSQPPPAPRWNQDWFPRLDAAAAYAAVRSL
                     KPKRIVEVGSGHSTRFLARAVADGGLDTRITAIDPQPRASIEKLAVEWLCTPVQRVAA
                     FPALGEGDILFIDSSHQLKPGSDVDFLLNAVLPMLPAGVRVHFHDIFLPDDYPEHWAW
                     RRYNEQPAVAALIRKDVFRVDFASHATVRFAPEKIRGVLERLPLVPGAIESSLWLT"
     gene            complement(634..1458)
                     /locus_tag="VFX94_04125"
     CDS             complement(634..1458)
                     /locus_tag="VFX94_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5767402.1"
                     /translation="MTFSVRFWGVRGSIACPGPRTARYGGNTSSLEVRCGERMLLFDA
                     GTGLRYLGNSLQSSGPIDADLFLTHTHFDHVAGLPFFKPFFKPHNRFRLWAGHLAEGM
                     TLRRVLGEFMMSPLFPVPPQVFRARMEYREFKAGDTLHPAEGIALRTAPLNHPDGATG
                     YRVDYGGRSICYLTDTEHVPGAPDRNILGLIAGADLVIYDSMYTDAEYDAYVGWGHST
                     WQEGVRLCRAAGAKRLAVFHHDPEHEDDMLDGVAREVEKELPGSIVARDGLVVGVD"
     gene            1606..>2607
                     /locus_tag="VFX94_04130"
     CDS             1606..>2607
                     /locus_tag="VFX94_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5767403.1"
                     /translation="MERSLFGFILKYSRLDQLRIVPFVLLTMVVYYASLDLPKTIINE
                     AIQGGRFPDGASTASFFGLGLGRLEYLFTLSMIFLALIVVNGWLKFRINTMKGWMGER
                     MLRRLRYALYDHILRFPLARFRRVKAAEMATMIKDEVEPLGGFIGEALITPLFLGGQA
                     LTAMFFILYQHWTLGLIALGVVGVQAFIIPRLRKRLLVLGRERQLTARALAGRIAETV
                     DGAAEIHANGTSNYERAEISARLGRIFGIRFEFYQRKFLIKFLNNFLSQVTPFLFYTV
                     GGYLVIVGGLDIGALVAVIAAYKDLPGPVKELIDWDQQRLDVQIKYTQVIEQFTVDDL
                     "
BASE COUNT          440 a          862 c          871 g          434 t
ORIGIN      
        1 gtgagccaca ggctcgattc gatggcgccg ggcacgaggg gcaggcgctc gagcacgccg
       61 cgaatctttt caggcgcgaa cctgacggtg gcgtggctgg cgaaatccac cctgaagaca
      121 tcctttctga tgagagccgc aacggccggc tgctcgttat agcgccgcca agcccagtgc
      181 tcgggatagt cgtcgggcag gaagatatcg tggaaatgca cgcgcacgcc cgccggcagc
      241 atcggcagca cggcgttcag caggaaatcg acgtcgctgc cgggcttcaa ctggtggctc
      301 gagtcgatga acaggatgtc gccctcgccc agcgccggaa acgcggccac ccgttggacc
      361 ggcgtgcaca gccactccac agcaagcttt tcaatggaag ccctcggctg cggatcgatc
      421 gccgtgatgc gcgtgtcgag gccgccgtcg gccaccgccc gggcgaggaa gcgcgtggaa
      481 tgccccgagc cgacctcgac gattcgtttc ggcttgaggc tgcgcacggc ggcgtacgcg
      541 gcagcggcgt ccaggcgcgg aaaccagtcc tggttccagc gcggcgctgg aggaggctgc
      601 gagcctatcg cctccagctc ggcggcgtag ctttcaatcc actccaacaa cgaggccatc
      661 cctcgcgacg atcgatcccg gcagctcttt ctccacctcg cgcgctacgc cgtcgagcat
      721 gtcgtcttcg tgctcggggt cgtggtggaa taccgcgagg cgcttcgccc cggcggcgcg
      781 gcacaggcgc acgccttcct gccaggtgga gtgcccccag ccgacgtacg cgtcgtactc
      841 ggcgtcggta tacatgctgt cgtagatcac gaggtcggcg ccggcgatga ggccgaggat
      901 gttgcggtcg ggagccccgg gcacgtgctc cgtgtcggta aggtagcaga tcgaacgccc
      961 gccatagtcg acccggtagc cggtcgcgcc gtcagggtgg ttgagcggcg ccgtgcgcag
     1021 cgcgatgccc tcggccgggt gcagcgtgtc gccggccttg aactcgcggt actccatgcg
     1081 cgcgcggaaa acctgcggcg gaaccgggaa tagcggcgac atcatgaatt cgccgagcac
     1141 ccggcgaagc gtcatgccct cggcgagatg ccccgcccag aggcgaaagc ggttgtgcgg
     1201 cttgaagaac ggcttgaaga agggcagccc ggcgacgtgg tcgaaatggg tatgcgtgag
     1261 aaaaaggtcc gcgtcgatcg gcccggagct ttgcagcgaa ttcccgaggt aacgcaggcc
     1321 ggtgccggca tcgaagagca gcatccgctc gccgcagcgc acctcgagcg acgaggtgtt
     1381 tccgccgtag cgcgccgtgc gcgggcccgg gcaggcaatc gagccgcgca cgccccagaa
     1441 gcggacactg aaagtcattc ccaactatag cttgtaaggg ccgcgattcg gggacggaac
     1501 cgattcggcg cacggagcac ctgacgcacc tcgcaagctg cggtcgggat tccgtccccg
     1561 aaccgcgctc gccgccttgt aagatgccgc ctacaaggac cccgcgtgga acgcagcctt
     1621 ttcggcttca tcctgaagta cagcaggctc gaccagctgc gcatcgtgcc gttcgtgctg
     1681 ctgacgatgg tggtgtacta cgcctcgctc gacctgccga agaccatcat caacgaggcg
     1741 atccagggcg gacgcttccc cgacggcgcg agcacggcga gcttcttcgg gctggggctt
     1801 ggccggctgg agtatctgtt cacgctgtcg atgatcttcc tcgccctcat cgtggtgaac
     1861 ggctggctga agttccgtat caacacgatg aagggctgga tgggcgagcg catgctgcgt
     1921 cggctgcgct acgcgctcta cgaccacatc ctgcgcttcc cgctcgcgcg cttccgccgc
     1981 gtcaaggccg ccgagatggc gaccatgatc aaggacgagg tcgagccgct cggcggcttc
     2041 atcggcgagg cgctgatcac gccgctcttc ctcggcggcc aggcgctcac ggcgatgttc
     2101 ttcatcctct accagcactg gacgctcggg ctgatcgcgc tcggcgtggt cggggtgcag
     2161 gcgttcatca tcccgcggct gcgcaagcgc ctgctcgtgc tcgggcgcga gcgccagctc
     2221 accgcgcgcg cgctcgccgg gcgcatcgcc gagaccgtgg acggcgccgc cgagatccac
     2281 gccaacggca cctcgaacta cgagcgcgcc gagatctcgg cgcgcctggg caggatcttc
     2341 ggcatccgct tcgagttcta ccagcgcaag ttcctgatca agttcctgaa caactttctt
     2401 tcgcaggtga cgccgtttct cttctacacc gtgggcggct acctcgtgat cgtcggcggg
     2461 ctggacatcg gcgctctggt ggcggtgatc gccgcctaca aggacctgcc cgggccggtg
     2521 aaggagctca tcgactggga ccagcagcgc ctggacgtgc agatcaagta cacgcaggtg
     2581 atcgaacagt tcaccgtgga cgacctg
//
LOCUS       DASUNT010000211         5509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_28050, whole genome shotgun sequence.
ACCESSION   DASUNT010000211 DASUNT010000000
VERSION     DASUNT010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28050"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VFX94_04135"
     CDS             complement(<1..522)
                     /locus_tag="VFX94_04135"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEX5767404.1"
                     /translation="MPTFQQLIAQLNAYWDRQGCALLQPYDMEVGAGTFHTATFLRAL
                     GPEPWNAAYVQPSRRPKDGRYGDNPNRLQHYYQYQVVLKPSPPDIQELYLKSLESLGV
                     NPREHDVRFVEDDWESPTLGAWGLGWEVWLDGMEITQFTYFQQAGSLELEPITAEITY
                     GLLRLCLYLQRVES"
     gene            complement(532..1977)
                     /gene="lnt"
                     /locus_tag="VFX94_04140"
     CDS             complement(532..1977)
                     /gene="lnt"
                     /locus_tag="VFX94_04140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX5767405.1"
                     /translation="MHFLSCVLAGAATVLAFAPFALHPLALLSFAVLVHFWMQAPPRR
                     CAWLGFAFGAGLFGAGVSWVYVSLHQFGGMAAPLAALATVGFCAFLALFPAAAGWLQA
                     RVPAPDAARACLLVPAAWTLAEWLLTWIFTGFPWLAIGYSAAGWPLQGYAPLGGVHAL
                     SFLTVSVAGMLWLLVRGKPGYLAVIVLVLGAGEALRHVAWTAPVGAPLAAALLQGNIA
                     QEMKFRPERAPRILDTYARLAEGSRARLIIFPETALPAFLHRIDPAYLARLEAVGRRN
                     EGDLLIGAPYLTARAYYNSAFTLGVSRRQIYHKVHLVPFGEFVPWGFRWTLDVLAIPL
                     SDFSRGSPEQPPLEIAAQRVAVNICYEDVFGAEIARSLPEATLLVNMSNVAWFGDSLA
                     PAQHLSIARLRAIETGRMHLTATNTGITAAIDRDGRVLARLEQFTEGRLDIAAQGYSG
                     ATPYVRFRDWPLVAACLFIFALFIFKAKRSR"
     gene            complement(1986..2810)
                     /locus_tag="VFX94_04145"
     CDS             complement(1986..2810)
                     /locus_tag="VFX94_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEX5767406.1"
                     /translation="MNDESRPSLLERLSSLLMREPGDRSELTQLLRSAYSRNLLDADA
                     LSIIEGALTVSEMAVRDIMVPRAQVDFIDINEPVETFVKQVIATAHSRFPVIDQNRDD
                     VIGVLLAKDLLRFYAGEEQFNVREMLRPAVFVPEAKRLNVLLREFRASRNHMAIVIDE
                     YGGVAGLVTIEDVLEQIVGDIEDEYDFDEASDNILPEPGGRHRVKALTQIADFNAAFG
                     TQLSDEAADTVGGYLIKHLGRLPKRGESVLIDGLRFQVLRADSRRVYTLLVDRPRT"
     gene            complement(2822..3211)
                     /gene="ybeY"
                     /locus_tag="VFX94_04150"
     CDS             complement(2822..3211)
                     /gene="ybeY"
                     /locus_tag="VFX94_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX5767407.1"
                     /translation="MPRRGVPPAASLRKWARAAIASPVTVRIVGEREGRRLNREFRQS
                     DRATNVLSFRHDDRTGDIVLCHPVIRREARAQGKSVAAHYAHLVVHGCLHLRGYDHEK
                     KRDAARMEAREIRILRRGGIADPYTVE"
     gene            complement(3231..4169)
                     /locus_tag="VFX94_04155"
     CDS             complement(3231..4169)
                     /locus_tag="VFX94_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX5767408.1"
                     /translation="MRAKPVEVKLDPLDNQRLARLCGALDENLRQIESALDVTIARRG
                     AKFTVKGEQAQRAAQAIEHFYDRAAGELTLEDVQLGLTELSKRISLKKEGPLLLTRRA
                     DLHGRTPHQVEYIEHILAHDLTFGIGPAGTGKTYLAVACAVDALERDKVKRIVLVRPA
                     VEAGERLGFLPGDLAQKVDPYLRPLYDALFDLMGFETTGKLLERGTIELAPLAYMRGR
                     TLNHAFIILDEAQNTTPEQMKMFLTRIGFGAKAVVNGDVTQIDLARGQKSGLIEARRI
                     LAGVRGIAFTQFGAQDVVRHPLVARIIDAYDSRQEE"
     gene            complement(4166..5482)
                     /gene="miaB"
                     /locus_tag="VFX94_04160"
     CDS             complement(4166..5482)
                     /gene="miaB"
                     /locus_tag="VFX94_04160"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041863071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX5767409.1"
                     /translation="MRKLYLRTFGCQMNEYDSDRITDVMREAEGLEPTPDPDDADLIV
                     FNTCSVREKAQEKVFTDLGRVKHLKRLRPGVMIAVGGCVASQEGAGIVERAPYVDVVF
                     GPQTLHRLPELLARRRSSGEPQVDVSFPEIEKFDRLPPARVQGASAFVSIMEGCSKYC
                     SFCVVPYTRGEEVSRPFDDVVAEVAGLAEQGVKEVTLLGQNVNAWRGAIAGAPADFAE
                     LLYYLDEVPGLERIRYTTSHPREFSPRLIGAHRDIGKLAPHVHLPVQSGSDRVLAAMK
                     RGYTALEYRSVVRRLRAARPGISVSSDFIVGFPGESEAEFHATLKLAREAQFDASFSF
                     VYSPRPGTPAAELPDAVSHPEKLERLQRLQRQLEAQARAISEAMVGTTERVLVEGPSR
                     KSSTELQGRTGNNRVVNFPGPEALINKYVELRITAALPHSLRGELA"
BASE COUNT          906 a         1812 c         1908 g          883 t
ORIGIN      
        1 cgactcgacg cgctgcaagt acaggcacaa gcgcaagagt ccgtaggtaa tctcagcggt
       61 gatgggctcc agctcgagcg agccggcttg ctggaaatag gtgaactgcg tgatctccat
      121 gccgtcgagc cacacttccc accccagccc ccaggcgccg agcgtgggcg actcccagtc
      181 gtcctcgacg aaccgcacat cgtgctcgcg cggattcacg cccaggcttt ccagggactt
      241 cagatacagc tcctggatgt cgggcggtga gggcttgagc accacctggt actggtagta
      301 gtgctgcaga cggttcggat tgtcgccgta gcggccgtcc ttcggccggc gcgacggctg
      361 cacgtacgcc gcgttccacg gctcggggcc gagcgcgcgc aggaatgtcg ccgtatgaaa
      421 ggtgcccgcg ccgacttcca tgtcgtaggg ctgaagcagc gcgcagcctt gccggtccca
      481 gtaagcgttc agctgcgcga tgagctgctg gaaagtcggc acgcgccgat tctaacggct
      541 gcgcttcgcc ttgaagatga acagcgcgaa aatgaaaagg caagccgcga cgagcggcca
      601 gtcgcgaaag cgcacatacg gcgtcgcgcc cgagtatccc tgcgccgcga tgtcgagccg
      661 gccttcggtg aactgctcca ggcgggcgag aacgcgtccg tcgcggtcga tggcggccgt
      721 gatcccggta ttcgtcgcgg tgagatgcat gcgcccggtc tcgatcgcgc gcaggcgcgc
      781 gatactgagg tgctgcgccg gcgcgagcga atcgccgaac caggcgacgt tcgacatgtt
      841 gacgagcagc gtcgcctcgg ggaggctgcg ggcgatctcg gcgccgaaca cgtcttcgta
      901 gcagatgttc accgcgacgc gctgcgccgc gatctcgagc ggcggctgct ccggggagcc
      961 gcggctgaag tccgacagcg ggatcgcgag cacgtcgagc gtccagcgaa agccccaagg
     1021 cacgaattct ccgaaaggca cgagatgcac cttgtggtaa atctgtcggc gcgacacgcc
     1081 gagcgtgaaa gcgctgttgt agtacgcgcg agcggtcagg tacggcgctc cgatgagaag
     1141 gtcgccctcg tttcgcctgc ccacagcctc caggcgcgcg aggtaggccg gatcgatgcg
     1201 atggagaaac gcgggaagcg cggtctcggg gaagatgatg agccgcgccc ggctgccttc
     1261 ggcgagccgc gcgtaagtat cgagtattct gggcgcgcgc tcgggccgga acttcatctc
     1321 ctgcgcgata tttccctgca gcagcgcggc cgcgagcggc gcgcccaccg gtgcggtcca
     1381 ggcgacgtgg cgcagcgctt cgcctgcgcc gagcacaagc acgatcacgg caagatagcc
     1441 gggcttgccc cgcacgagga gccagagcat tccggcgacc gagaccgtca gaaaggaaag
     1501 cgcatgcacg ccgccaagcg gcgcgtagcc ctgcagcggc catcccgcgg ccgagtatcc
     1561 gatcgcgagc caggggaatc cggtgaagat ccatgtgagc agccattcgg cgagcgtcca
     1621 cgcggccgga acgagcaggc acgcgcgcgc cgcatcgggc gcgggaacgc gcgcctgcag
     1681 ccagcccgcc gccgcaggga aaagcgcgag gaatgcgcaa aacccgaccg tggcgagcgc
     1741 ggcgagcggc gccgccatgc cgccgaactg gtgaaggctc acgtagaccc acgacacgcc
     1801 cgcgccgaag agccctgcgc caaaggcaaa gccgagccag gcacagcgcc gcggcggcgc
     1861 ctgcatccag aaatggacga gcacggcgaa gctcagcaag gcgagcgggt gcaacgcgaa
     1921 tggcgcgaac gcgagcaccg tcgccgcgcc cgcgagcacg caggacagga aatgcaaagc
     1981 cggggtcagg ttctcggccg gtcgaccagg agcgtgtaca cgcgccggct gtctgcgcgc
     2041 agcacctgga agcgcaggcc gtcgatcagc accgactcgc cgcgcttcgg caggcggccg
     2101 aggtgcttga tgagatagcc gccgacggta tcggccgcct cgtcggagag ctgggtgccg
     2161 aaggcggcgt tgaaatccgc gatctgcgtg agcgccttca cgcgatgccg gccgccgggc
     2221 tcgggaagaa tgttgtcgga ggcctcgtcg aagtcgtact cgtcctcgat gtcgccgacg
     2281 atctgctcga ggacgtcctc gatcgtcacc aggcccgcga cgccgccgta ctcgtcgatc
     2341 acgatcgcca tgtggttgcg gctcgcgcgg aactcccgca gcagcacgtt caggcgcttc
     2401 gcctcgggca cgaacaccgc cggccgcagc atctcgcgca cgttgaactg ctcctcgccc
     2461 gcataaaagc gcagcaggtc cttggcgagg agcacgccga tcacgtcgtc gcggttctgg
     2521 tcgatcaccg ggaagcgcga gtgcgcggtg gcgatcacct gcttcacgaa agtctccacc
     2581 ggctcgttga tgtcgatgaa gtcgacctgg gcgcgcggca ccatgatgtc gcgcaccgcc
     2641 atctcggaga cggtgagcgc gccttcgatg atggagagcg cgtccgcgtc gagcaggttg
     2701 cgcgagtagg ccgacctgag aagctgcgtg agttcgctgc ggtcgccggg ctcgcgcatg
     2761 agcagcgacg acaggcgctc gagcaggctc ggtcgggact cgtcgttcat gcctggcgcg
     2821 ttcattctac cgtgtaggga tcggcgattc cgccgcggcg caggatgcgg atctcgcgcg
     2881 cttccatgcg cgccgcatcc cttttctttt catgatcgta gccgcgcagg tggaggcagc
     2941 cgtgcaccac gagatgcgca tagtgcgccg caacgctttt gccctgcgcg cgcgcctcgc
     3001 gccggatgac ggggtggcac agcacgatgt caccggttct gtcgtcatgg cgaaagctga
     3061 gcacgttggt ggcgcgatcg ctctgcctga actcgcggtt gagacgccgg ccttcgcgct
     3121 cgccgacgat cctcacggtg accggggaag cgatcgccgc gcgcgcccac ttgcgcagag
     3181 acgcggcggg gggaacgccg cgccgcggca cggcatattg aatgaagacc ctactcttcc
     3241 tgccgcgagt cgtaggcatc gataatgcgc gccaccagcg ggtggcgcac gacgtcctgc
     3301 gcgccgaatt gcgtgaaggc gatgccgcgc acgccggcga ggatgcggcg cgcctcgatg
     3361 aggccgctct tctggccgcg tgcgaggtcg atctgcgtga cgtcgccgtt gaccaccgcc
     3421 ttggcgccga agccgatgcg ggtgaggaac atcttcatct gctccggggt ggtgttctgc
     3481 gcttcgtcga ggatgatgaa cgcgtggttg agcgtcctgc cgcgcatgta ggcgagcggc
     3541 gcgagctcga tcgtgccgcg ctcgaggagc ttgcctgtcg tctcgaagcc catcaggtcg
     3601 aagagtgcgt cgtacagagg ccgcaggtag ggatcgacct tctgcgcgag gtcgccgggg
     3661 aggaagccca ggcgctcgcc cgcctcgacc gccggccgca cgagcacgat gcgcttcacc
     3721 ttgtcgcgtt cgagcgcgtc caccgcacag gccaccgcga ggtaggtctt gccggtgccc
     3781 gccgggccga ttccgaaagt gagatcgtgc gccaggatgt gctcgatgta ctccacctgg
     3841 tgcggtgtgc ggccgtgcag gtcggcgcgc cgggtgagaa gcaggggccc ttctttcttg
     3901 agcgagatgc gcttcgacag ctccgtcagc ccgagctgca cgtcctcgag cgtcagctcg
     3961 ccggccgcgc ggtcatagaa atgctcgatc gcctgcgccg cgcgctgcgc ctgctcgccc
     4021 tttacggtga atttcgcgcc gcggcgggcg atcgtgacgt cgagcgcgga ctcgatctgg
     4081 cgcagattct cgtcgagcgc cccgcacagg cgggcgaggc gctggttgtc gagcggatcg
     4141 agcttgactt cgaccggttt ggccctcagg cgagctcacc ccgcaggctg tgagggagcg
     4201 cggcggtaat gcgcaattcc acgtacttgt ttatgagggc ctcgggcccg gggaagttca
     4261 ccacgcggtt attcccggtg cggccttgca gctcggtgct gcttttccgg gaagggccct
     4321 ccaccagcac gcgctcggtc gttccgacca tggcctcgct gatggcgcgc gcctgcgctt
     4381 cgagctgccg ctgcaggcgt tgcagcctct cgagcttctc ggggtgagaa acggcatcgg
     4441 gcaactcggc tgccggggtt ccgggccgcg ggctgtagac gaaggagaat gaagcgtcaa
     4501 attgcgcttc gcgcgcgagc ttgagcgtcg cgtgaaactc cgcttcgctc tcgcccggga
     4561 atccgacgat gaagtccgac gacacactga tgccgggccg cgccgcgcgc aggcgccgca
     4621 cgacggagcg gtactcgagc gcggtgtagc cgcgcttcat cgcggcgagc acgcggtcgg
     4681 agccggactg caccggtaga tgcacgtggg gagcgagctt gcctatgtcg cggtgcgcgc
     4741 cgatcagccg cgggctgaat tcccggggat gcgaagtcgt atagcggata cgctccaggc
     4801 ctggcacttc gtccaggtag tagagcagct ccgcgaagtc cgcgggcgcg ccggcgatcg
     4861 cgccgcgcca ggcgttgacg ttctgcccga gcagcgtcac ttctttcacg ccctgctcgg
     4921 caagcccggc cacttccgcc actacatcat cgaagggccg cgacacctct tcgccgcgcg
     4981 tgtagggcac gacgcagaag ctgcagtact tgctgcaccc ttccatgatc gaaacgaagg
     5041 ccgaggcgcc ctggactctt gccggcggca ggcggtcgaa tttctcgatc tccggaaagc
     5101 tcacgtcgac ttgcggctcg ccgctcgagc ggcggcgcgc gagcagctcg ggcagccggt
     5161 gcagggtttg cgggccgaac acgacgtcga cgtacggcgc gcgctcgacg atgcccgcgc
     5221 cttcctggct cgctacgcag ccccccacag caatcattac cccggggcgc aagcgcttga
     5281 ggtgcttcac ccgcccgagg tcggtgaaga ctttctcctg cgctttctcg cgcaccgagc
     5341 aggtgttgaa gacgatgagg tccgcgtcgt cggggtccgg cgtcggctcc aggccttcgg
     5401 cttcgcgcat cacgtccgtg atccggtcgg agtcgtactc gttcatctga cagccgaacg
     5461 tgcgaaggta gagcttcctc atttcgtcgg cgcgggcgcg ggcggcagc
//
LOCUS       DASUNT010000212         5226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1256725, whole genome shotgun sequence.
ACCESSION   DASUNT010000212 DASUNT010000000
VERSION     DASUNT010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256725"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1199
                     /locus_tag="VFX94_04165"
     CDS             168..1199
                     /locus_tag="VFX94_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5767410.1"
                     /translation="MAEPFAHLVLDCVTAVYGDTTAVESLSLEVGRGELVGLLGPSGC
                     GKTTTLRIVAGLVAPSSGRVLVNGQDVTGLPAHRRNIGMMFQSYALFPHFTVIENVAF
                     GLRMRHVPRAERRRRAIAALELVELGGFGERYPAQLSGGQQQRVALARALVIEPTLLL
                     LDEPLSNLDAHLRAEMRDEIRALQQRLSITTLFVTHDQEEALAVSSRVAVLNRGRLVE
                     VGTPQGLSDHPAYPFTAAFLGARTVIEGRASGGVFRAPGLACGGAPEGARRIILRAAR
                     LRLDAETGPLRLDGRVASVTFIGDAFETDVDTEAGRVRVLVPSLTPPPPVGAACRISA
                     MPGGASFIS"
     gene            1225..2262
                     /locus_tag="VFX94_04170"
     CDS             1225..2262
                     /locus_tag="VFX94_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5767411.1"
                     /translation="MTRREFGKLALAAGAAAPFSIVRTALAQPKPGDELVVGIWGGAQ
                     ERIVRQHVEKALVAKFGCKVSYVLSATPERRARAYAERGRPSFDVIYLNIFESRQAVK
                     DGVTQPPSGAVAQFANLYEIARVGGYGVAFNPVTIVYDRRKASRRVTSWKDIWNPEWK
                     GRIAWPVYPGAQGTAGLLMAAKVWGGSEKSIDIGFQKIRELRPFAAIQQSPDQLFQMF
                     DQGVCDLSIDFGSLARSYAETRNPNLEIANPVEGQMVAMNVACITVGTMNQKLAEEWI
                     NLHLSPPCMLAYAREIYYSPTVRNIEIPAELKPKLVLAEDVNKLVDFDWEHVIRMQSQ
                     WSSRFNREIAG"
     gene            2259..3947
                     /locus_tag="VFX94_04175"
     CDS             2259..3947
                     /locus_tag="VFX94_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5767412.1"
                     /translation="MTARAGPALAAPATAWLLATFAAPFGVVILLSLQPHADPFAPLF
                     TQPSAAQFRAILTDSFYLRVILETLALGAGVTLLAVLLGYPVALWLARMPARWRAIAL
                     AVILVPLLTNVVVRSLGIVLLLAPEGLINQVLGVFGLGPIRGMLYPHGAVALALAQAF
                     MPFVVLALYDVLQRISPGVHEAAQSLGASPAVRFWTVDFPLSLPGLRAGVMIVFLVSS
                     TAYVSATLLGGKKVWTTGMLVWEEALQNLNAPTASALALIMTACSIAFIAACGFAFAR
                     LTPWLAGRPAEAWSPPARLVRALDLAAPAVYRLLLAAALALLLLPLVLVCIQGFNDVP
                     QATVTGFRAFTWKWYQAVLFGGAYVDSFRLSIEIAVVSATVAVALAIPAAFALARHRF
                     TGLAVLAGLWALPLSLPLVAIGVGMLRLLQIYTAVPPFLGIAAVHVVLILPFSIFLLQ
                     ASVQRLERAQEEAAATLGAGALRRFALVIVPGLAPGLAATAIIAFLLSFGEVTVTSFL
                     TTARTITLPVRIYAEASFSLEPTVHAISTLLIMVTIVALAALNRAVRLDRLHVS"
     gene            4017..>5226
                     /locus_tag="VFX94_04180"
     CDS             4017..>5226
                     /locus_tag="VFX94_04180"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5767413.1"
                     /translation="MPKTSVLEGLSPETQLAGEHRVLEMIAARKPLGETLDALCRLVE
                     QASPEWLASILLVDAEADSVWHGAAPTLPAAYVSALDGERIGPAYGPCGLAALGNQAL
                     AADIATDSRWSAEYRALALENGLRACWSTPVKASNGRVLGVLEIYLRQPGDPGASGLS
                     LEPFAHLAAIVIERAQAMPADVGGELLDELRRSKEELQRLMASVSDYLWSAEVALDGS
                     YTYRYYSPVVERITGWPAEKLLESRERWVELLHPLDRRRVDETFRSLMSGATEHVEVE
                     YRIVRPDGTLRWVRDSAHGTRVEDGRILLYGVVSDITERKRGAEALRESEARFRALTE
                     LSSDWYWEQDENLRFTYLSSQANEVTGHASESSLGRTRWELSGVTPISSSWAEHQAAL
                     AAHQSFRDFEY"
BASE COUNT          737 a         1825 c         1755 g          909 t
ORIGIN      
        1 cggggcactg tggtggggcg cggcgcggcg cccgcgcacc acgatggtct ccgccgcctt
       61 gaagaagaat gccctcgatc atgtctccgc ttacggtcag gccgagcgcg cttgaccggc
      121 tctgttgcga tgcttagcct gctccgaccc tggagtggga tggaccaatg gcggagccgt
      181 tcgctcacct cgttcttgac tgcgtcacgg ccgtctacgg cgacacgacc gccgtcgagt
      241 cgctctcgct cgaagtcggg cgaggagagc tggtcgggct gctgggtccc tccggatgcg
      301 gcaagacgac cacgctcagg atcgtcgccg gcctggtcgc gccctcatcc gggcgcgtgc
      361 tcgtgaacgg ccaggacgtg accggcctgc ccgcccatcg acgcaatatc ggcatgatgt
      421 tccagtccta cgcgctcttt ccgcatttca cggtcatcga gaacgtggcc ttcggcctgc
      481 gcatgcgcca cgtgccccgg gcggaacggc ggaggcgggc catcgcggcg ctcgagctgg
      541 tcgagctcgg cggcttcggg gagcgctatc cggcgcagct ttcgggaggc cagcagcagc
      601 gcgtggcgct cgcccgcgcg ctggtgatcg agccgacgct gctcttgctc gacgagccgc
      661 tctccaatct cgatgcccat ctgcgcgccg agatgcgcga cgagatccgc gcgctccagc
      721 agcgcctttc catcaccacg ctcttcgtga cccacgatca ggaggaggcg ctcgccgtgt
      781 cgagccgcgt cgccgttctc aatcgcgggc ggctcgtcga ggtcggaacg ccgcaaggcc
      841 tttccgacca tccggcgtat cccttcactg ccgccttcct cggcgcgcgc accgtgatcg
      901 aaggccgcgc gagcggtggc gtgttccgcg ctccgggcct cgcctgcggg ggtgcgccgg
      961 aaggggcgag gcgcatcatc ctgcgcgcgg cgaggctgcg cctggacgcc gagaccggac
     1021 cgctccggct cgatggccgc gtggcttcgg tcaccttcat cggcgacgcc ttcgagaccg
     1081 acgtggacac cgaagccggc cgggtccggg tccttgtccc ctcgctcacg ccgccgcccc
     1141 ccgtcggtgc cgcctgccgg atcagcgcca tgccgggcgg cgcgagcttc atcagctaga
     1201 aaaagaagga gaccgacgtt gtcgatgacg cgccgcgaat tcgggaagct cgcgctggcg
     1261 gccggcgccg ccgcgccgtt ctccatcgtc cgcaccgcgc tcgctcagcc gaagccgggc
     1321 gatgagctcg tcgtcgggat ctggggcggc gcccaggagc gcatcgtcag gcagcatgtg
     1381 gaaaaggcgc tcgtcgcgaa gttcggctgc aaggtgagct acgtgctgag cgcgacgccg
     1441 gagcggcgcg cgcgagccta tgcggagcgc ggccggccaa gcttcgacgt catttatctc
     1501 aacattttcg aaagccgcca ggcggtcaag gacggcgtga cgcagccgcc gagcggcgcg
     1561 gtggctcagt tcgccaatct ctatgagatc gcgcgggtcg gcggatatgg cgtcgccttc
     1621 aacccggtga cgatcgtcta tgaccggcgc aaggcttcca ggcgggtcac gtcctggaag
     1681 gacatctgga acccggaatg gaaaggccgc atcgcctggc ccgtctatcc cggcgcgcag
     1741 ggaaccgccg gcctgctgat ggctgcgaag gtctggggcg gaagcgagaa gagcatcgac
     1801 atcggcttcc agaaaatcag ggagctgagg cccttcgcgg ccatccagca gagcccggat
     1861 cagctcttcc agatgttcga tcagggggtg tgcgatctct cgatcgactt cggcagcctg
     1921 gcgcgcagtt acgccgagac gcgaaacccg aacctcgaga tcgcgaatcc ggttgaaggc
     1981 cagatggtcg cgatgaacgt cgcctgcatc accgtcggca ccatgaacca gaagctcgcc
     2041 gaggagtgga tcaacctgca cctcagcccg ccctgcatgc tcgcctatgc gcgcgagatc
     2101 tactactcgc ccacggtcag gaacatcgag attccggcgg agctcaagcc gaagctcgtg
     2161 ctggcggagg acgtgaacaa gctcgtcgat ttcgactggg agcacgtgat ccgcatgcag
     2221 tcgcagtggt cgagccgctt caaccgcgaa atcgccggtt gacggctcgc gccgggccgg
     2281 cgctcgcggc gccggccacc gcctggcttc tcgccacgtt cgccgcgccc ttcggcgtgg
     2341 tgatcctgct ttcgctccag ccgcatgccg atccgttcgc gccgcttttc acgcagccga
     2401 gcgcggcgca gttccgcgcc atcctgaccg attccttcta cctccgggtg atcctggaga
     2461 cgctcgcgct cggcgcggga gtaacgctgc tcgccgtcct cctcggctat ccggtcgcct
     2521 tgtggctcgc gcgcatgccg gctcgctggc gagcgatcgc cctcgccgtg atcctggttc
     2581 cgctgcttac caacgtggtc gtgcgctcgc tcggcatcgt gctcctgctg gcgccggaag
     2641 ggctgatcaa ccaggtcctg ggcgtattcg gtctcggccc gatccgcggc atgctctacc
     2701 cgcacggggc ggtcgcgctc gcgctcgcgc aggccttcat gcccttcgtg gttctcgcgc
     2761 tttacgacgt gctgcagcga atctcgccgg gcgtgcacga ggctgcacaa agcctcggcg
     2821 cctcgcccgc ggtgcggttc tggaccgtgg atttcccgct ctcccttccg gggctgcgcg
     2881 ccggggtgat gatcgtcttc ctcgtgtcgt ccacggccta tgtgtcggcc acgctcctcg
     2941 gaggcaagaa ggtctggacc accggcatgc tggtctggga ggaagcgctg cagaacctga
     3001 acgcgccgac cgcgtccgcg ctcgccctga tcatgaccgc gtgcagcatc gcattcattg
     3061 cggcgtgcgg cttcgccttc gcgcgactga cgccgtggct cgccggccgg cccgccgagg
     3121 cgtggagccc gcccgcgcgc ctcgtgcgcg cgctcgatct cgccgcgccc gcggtctatc
     3181 ggttactgct cgccgcggcc ctcgcgctgc tcctgcttcc tctcgttctc gtctgcatcc
     3241 agggcttcaa tgacgtgccg caggcgacgg tgacgggctt tcgtgccttc acctggaagt
     3301 ggtaccaggc ggtcctcttc ggcggcgcct acgtggactc gttccggctg tcgatcgaga
     3361 tcgcggtcgt ctcggccacg gtggcggtcg ccctggcgat ccccgccgcc ttcgcgctgg
     3421 cgcgccacag gtttacgggt ctcgctgtgc tggcggggct gtgggctctc ccgttgtcgc
     3481 tgccgctggt cgccatcgga gtcggaatgt tgcgtctgct gcagatctac acggcggtgc
     3541 cgccgttcct ggggatcgct gcggtgcacg tcgtgctcat cctgcccttc agcatcttct
     3601 tgcttcaggc gtcggtacag cggctcgagc gcgcgcagga ggaggccgcc gccaccctcg
     3661 gggccggagc cctgcgtcgc tttgcgctcg tcatcgttcc cgggctcgcg cccggcctcg
     3721 ccgccacggc catcatcgcg tttctgctga gcttcggcga ggtgaccgtc accagcttcc
     3781 tcacgaccgc gcgcaccata acgctgccgg ttcgcatcta tgccgaagcg agcttcagcc
     3841 tcgagccgac agtgcatgca atctcgaccc ttctcatcat ggtgacgatc gtggcgctcg
     3901 cggcgctcaa ccgggcagta cggctcgatc ggcttcatgt cagttgacac aatcggccat
     3961 tcgaccgtcg cgatgacggc tcgcgcggga ggtcgaatca tcatgggaag gtgcgcgtgc
     4021 cgaaaaccag tgtgctggaa ggattaagtc ccgaaacgca gctcgccggc gagcaccgcg
     4081 tgctcgagat gatcgcggcg cgcaagccgc tcggcgaaac gctcgatgcg ctgtgccgcc
     4141 tggtcgagca ggcctcgccc gaatggcttg cctcgattct gctcgtcgac gccgaggcgg
     4201 acagcgtctg gcacggcgcg gcaccgacgc ttcccgcggc ctacgtgtcg gcgctcgacg
     4261 gcgagcgcat cggcccggcg tacggcccgt gcgggctcgc cgcgctggga aatcaggcgc
     4321 tcgctgccga catcgccacc gactcccggt ggtccgccga gtaccgcgcg ctcgcgctcg
     4381 agaatggcct gcgtgcgtgc tggtcgacgc cggtcaaggc gtcgaacggc agggtgctcg
     4441 gcgtgctcga aatctacctg cgccagccgg gcgacccggg cgcatccggg ctcagtctcg
     4501 agcccttcgc tcacctcgcg gcaatcgtca tcgagcgcgc gcaggccatg ccggccgacg
     4561 tcgggggaga gctactcgac gagcttcgac gttccaagga agagctgcag cggctgatgg
     4621 catccgtctc ggactacctc tggagcgccg aggtcgcgct cgacggttcg tacacctatc
     4681 gctactactc gccggtggtg gagcgcatca ccggctggcc ggccgagaaa ctgctcgagt
     4741 cgcgcgagcg ctgggtggag ctcctccatc ccctcgaccg gcggcgcgtc gacgagacct
     4801 tccgcagcct catgtcgggc gccaccgagc atgtggaggt ggagtaccgc atcgtgcggc
     4861 ccgacggcac gctgcgctgg gtgcgcgaca gcgcgcacgg gacgcgggtc gaggacgggc
     4921 gcatcctcct ttacggcgtg gtgagcgaca tcaccgagcg caagcgcggc gccgaggccc
     4981 tgcgcgagag cgaagcacgc ttccgcgcgc tgaccgagct gtcctccgac tggtactggg
     5041 agcaggacga gaacctgcgc tttacgtacc tctcgagcca ggcgaacgag gtgaccggcc
     5101 acgcgagcga gtcctcgctc ggcaggacgc gctgggagct ctcgggcgtg acaccgatct
     5161 cgagctcctg ggcagagcac caggcagcgc tcgcggctca tcagtcgttc cgcgacttcg
     5221 agtatt
//
LOCUS       DASUNT010000213         9676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_467113, whole genome shotgun sequence.
ACCESSION   DASUNT010000213 DASUNT010000000
VERSION     DASUNT010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9676
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467113"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..912
                     /gene="mlaE"
                     /locus_tag="VFX94_04185"
     CDS             97..912
                     /gene="mlaE"
                     /locus_tag="VFX94_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEX5767414.1"
                     /translation="MAELTVIAALRTRLERLGSAVTSIVWRLGFASRFLTYLVVHSGV
                     ALRRFRLTIAEVYFAGVLSLVIILVSGLFVGMVLALQGYETLQRFGASESLGVLVALS
                     LVRELGPVVAGLLFASRAGSAITAEIGLMKATEQISAMEMMAVDPIARVVAPRFWGGV
                     ISMPLLAALFSAAGIFGGYLVGVQLIGVDAGSFWSQMQAAVDVREDILNGVIKSLVFG
                     VAVAWIAVFEGYDAVPTAEGVSRATTRTVVTSSLAILALDFVLTALMFTGGNH"
     gene            913..1386
                     /gene="mlaD"
                     /locus_tag="VFX94_04190"
     CDS             913..1386
                     /gene="mlaD"
                     /locus_tag="VFX94_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEX5767415.1"
                     /translation="MNRSMIDLWVGIFVAAGFGALLFLALKVGNLATFSTDQSYQVQA
                     KFANIGGLKVRGPVKSAGVVVGRVTDVRFDNESYEAIVTMTLDHGYQFPRDTTAKILT
                     SGILGEQYVGLEAGGDGVMLKNGDRLRLTQSAVVLENLISQFLFNKATEAKPQDK"
     gene            1383..2093
                     /locus_tag="VFX94_04195"
     CDS             1383..2093
                     /locus_tag="VFX94_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEX5767416.1"
                     /translation="MTPVRHATLTAALCLLLCSGCAGVEGERDPRDPWEGLNRGVYRF
                     NETVDEYVAQPLARAYVAVLHQEIRTRVSNFFANIQDLLIGANNLLQGKFEDGITDWA
                     RFVFNSSFGLFGLHDFASDAGLEKHNEDFGQTLGRWGAGPGPYLVLPFLGSSTVRDAA
                     GTAVDWAVQPVGEVRPIALRNSLYGLYFVDTRAQLLDASRILEEAALDKYVFQRDAYL
                     QRRRSLIYDGRPPREKIE"
     gene            2090..2704
                     /locus_tag="VFX94_04200"
     CDS             2090..2704
                     /locus_tag="VFX94_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5767417.1"
                     /translation="MTRMLLKFALVAFAAAGSPLGAQELAPDALVKNVTLEVVEMISK
                     DKEIKAGSRAKLVALVEEKVLPHFNFTAMTALAMGQNWAKATPEQKKQLTEQFRTLLV
                     RTYASALAAYSDQKFDFRPLRAKPTDTDVTVNVRVLQPGAQPVTLDYSMEKTANGWKV
                     YDVMVAGVSLVANYRTEFANTVRASGVDALIRDLQTKNKSMEKK"
     gene            2701..2985
                     /locus_tag="VFX94_04205"
     CDS             2701..2985
                     /locus_tag="VFX94_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5767418.1"
                     /translation="MIRREGRRITLSGPVTLANAAQLLEQGRQHFAEGARTVDLGEVT
                     ELDSALLALVLQWLREARAAKRELALANLPQSLDTLARLYGVQDLLPVAR"
     gene            2982..3836
                     /locus_tag="VFX94_04210"
     CDS             2982..3836
                     /locus_tag="VFX94_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5767419.1"
                     /translation="MKAIEVRGVDKRYGTLRALSEVSLAIDTGEFFGLLGPNGAGKTT
                     LINVIAGLARPDAGSASVMGADVARDYRRARRMLGVVPQELVFDPFFTVRETLRIQAG
                     YFGIRGNDAWIEEVMHHLDLAEKAQANMRSLSGGMKRRVLVAQALVHKPPVIVLDEPT
                     AGVDVELRQGLWDFVRRLNRDGHTIVLTTHYLEEAETHCSRIAVLKAGRVVALDSTRN
                     LLAASSGSRISARVVGRDGRQHLLQIEGYAQLERELARLRESGVEIADLEIERPDLEQ
                     VFLRLTAE"
     gene            3833..4585
                     /locus_tag="VFX94_04215"
     CDS             3833..4585
                     /locus_tag="VFX94_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5767420.1"
                     /translation="MSFWTLFTKELLRFLKVSVQTVAAPVLTSLLYLMIFGQVLEGRL
                     RVFEAVPYTSFLVPGLVMMSVLQNAFANSSSSLIQSKITGNIVFVLLAPLSHAEFFWA
                     YVAAAVARGLAVGAGVLTCTSAFVELELQAPVWAAAFAVLGGALLGALGLLAGIVSEK
                     IDQLAAFQNFVILPLTMLSGVFYSIHSLPGFWREVSQANPFFYMIDGFRYGFFAASDV
                     APGISLAVVAVAFVAVAWVCLRLLKSGYRLRT"
     gene            4586..4825
                     /locus_tag="VFX94_04220"
     CDS             4586..4825
                     /locus_tag="VFX94_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX5767421.1"
                     /translation="MVTADSVKRGIESGLACEHVEVVGDGQHFQALVVSAEFAGRNRV
                     QRHQLVYAALGDRMREEIHALSMQTLTPDEWKGRG"
     gene            4818..6068
                     /gene="murA"
                     /locus_tag="VFX94_04225"
     CDS             4818..6068
                     /gene="murA"
                     /locus_tag="VFX94_04225"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX5767422.1"
                     /translation="MDKLRIIGGKPLEGEIRISGAKNAALPIMCAALLTAKPLTLTNV
                     PALRDVSTMAKLLGQMGLRVDRSPARVALHAASIEDPTASYDLVKTMRASVLVLGPLL
                     ARCGRAKVSLPGGCAIGARPVDQHVKGLEAMGATIAIEHGYMHATAKRLRGARIVMDL
                     VTVTGTENLMMAAALAEGETLIENAAREPEVVDLARCLTAMGAKIEGAGSAVIRITGV
                     GSLDGAEHRVMPDRIETGTYLAAATASGGNVRLIGAAPDSLDATLDKLREAGARIATS
                     ADSIEIRMSERPNAVSVRTAPYPGFATDMQAQFMALATVARGTAVITETIFENRFMHA
                     LEMQRLGADIRLEGNTAIVNGVERLQGARVMATDLRASAGLVIAGLVATGETLVDRIY
                     HLDRGYEALETKLSALGARVERVR"
     gene            6131..6775
                     /gene="hisG"
                     /locus_tag="VFX94_04230"
     CDS             6131..6775
                     /gene="hisG"
                     /locus_tag="VFX94_04230"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX5767423.1"
                     /translation="MASISIALSKGRIFDETAPLLARAGIRPKADPDSSRKLIIGTNR
                     RNVRLVVVRATDTPTYVQHGGADLGIAGRDVLAEHGAEGLYQPVDLGIAACRMMVAVP
                     RGFDYASAVRQGARLRVATKYVNTARAHFAAKGVHVDLIRLYGSMELAPLVGLADAIV
                     DLVSTGRTLKANGLVAVEELMPVSARLIVNQAALKTKRAELQPLIDAFARAARR"
     gene            6772..8034
                     /gene="hisD"
                     /locus_tag="VFX94_04235"
     CDS             6772..8034
                     /gene="hisD"
                     /locus_tag="VFX94_04235"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX5767424.1"
                     /translation="MKLRRLSTRARGFEAKLAALTRYEAAQDPALQKVVRKILADVRK
                     RGDAAVRTYTRKFDGVAPGQYAVSDKLLDAIPADQAQALRAAHERIRAFHEKQLQQSW
                     HFTDADGTRLGQQVSPLQRVGLYVPGGKAAYPSSVLMNAVPAKVAGVGELIMASPNPS
                     ALVLAAAALAGVDRVLGIGGAQAIAALAYGTQSIPRVDKIVGPGNAYVAEAKRQVFGE
                     VGIDMVAGPSEILIIADASVNPDWIAMDLFSQAEHDESAQAILLCPDEKYLEAVAGSI
                     AKLIAEMPRRKVIEASLKSRGALIRTRDLAEACAVANRIAPEHLELAVQDPEKILASV
                     RTAGAVFLGQWSSEAIGDYCAGPNHVLPTAGTARFSSPLGVYDFQKRTSVIGVSKQAA
                     AKLGRVAVTLAEGEGLSAHARAAQMRFE"
     gene            8031..9089
                     /gene="hisC"
                     /locus_tag="VFX94_04240"
     CDS             8031..9089
                     /gene="hisC"
                     /locus_tag="VFX94_04240"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX5767425.1"
                     /translation="MTPEQLVRAEILALKAYHVAPAEGMVKLDAMENPYPLPPAVRRE
                     LAEALARVDLNRYPDPAAPKLRELIARKMGVPPGMELLLGNGSDDLIQMVTLSLARPG
                     AVMMYPAPTFVMYAMNATYFGMRTVATGLTEDFSLDAAAFIRAMRKENPALIYIAYPN
                     NPTGVLYAEDDIVAILNEAPGLVVLDEAYHVFARKTLMPRLVQFSNLVVMRTVSKLGL
                     AGIRLGYLAARPQWVEQFNKVRQVYNVNVLTQTAAAFMLERIEVLEEQAAKIRAEREK
                     MGQSLKALHGVTVFPSEANFFLVRVPDADRTFEGLKRQGVLVRNLNSPALKNCLRITV
                     GTPDENRILLNALKEALQ"
     gene            9100..>9676
                     /gene="hisB"
                     /locus_tag="VFX94_04245"
     CDS             9100..>9676
                     /gene="hisB"
                     /locus_tag="VFX94_04245"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX5767426.1"
                     /translation="MRTAEVTRTTKETQIRVKLNLDGKGTARLSTGLPFLEHMLDQVA
                     RHGMLDLDIEAKGDLHIDAHHTVEDIGITLGQALAKAIGDKAGVRRFGHAYVPLDEAL
                     SRVVLDFSGRPGLEYHVKFTRALIGEFDVDLVHEFFQGFVNHAQLTLHIDNLRGDNAH
                     HQCETMFKAFARALRMAAEPDPRAAGSVPSTK"
BASE COUNT         1621 a         3218 c         3220 g         1617 t
ORIGIN      
        1 accccttcgt caaccagttc gtgaacggca agcccgacgg cccggtgccg ttccactacg
       61 cggcaccgga ctacgcggcg gacctcgagc tcgctgttgg ctgagctcac cgtgatcgcg
      121 gcgctgcgca cacgcctgga gcgcctcggc agcgccgtca cctcgatcgt ctggcggttg
      181 ggctttgcca gccgctttct gacctacctg gtggtccact ccggtgtcgc gctgcgccgc
      241 ttccggctca cgatcgccga ggtctacttc gccggggtgc tctcgctcgt catcatcctg
      301 gtgtcgggcc tgttcgtcgg catggtcctc gcgctgcagg gctacgagac gctgcagcgc
      361 ttcggcgcgt cggaatcgct cggcgtgctc gtcgcgctgt cgctcgtgcg ggagctcggc
      421 ccggtggtgg cgggactgct cttcgcgagc cgcgcgggct ccgcgatcac ggccgagatc
      481 ggcctgatga aggcgaccga acagatctcg gcgatggaga tgatggcggt cgacccgatc
      541 gcgcgggtgg tggcgccgcg cttctggggc ggggtgatct cgatgccgct cctcgcggcg
      601 ctgttctccg cggccggcat cttcggcggc tacctcgtcg gcgtgcagct gatcggcgtc
      661 gacgccggct cgttctggtc gcagatgcag gcagccgtcg atgtacgcga ggacatcctg
      721 aacggcgtga tcaagagcct ggtgttcggc gtggcggtgg cctggatcgc ggtgttcgag
      781 ggttacgacg ccgtgccgac cgccgaaggc gtatcgcgcg cgaccacgcg cacggtggtg
      841 acctcatcgc tcgcgatcct ggcgctcgac ttcgtcctca ccgcgctcat gttcaccgga
      901 ggcaaccatt agatgaaccg ctcgatgatc gatctgtggg tcggcatctt cgtcgccgcc
      961 ggcttcgggg cgctcctttt ccttgcgctc aaagtaggca acctcgccac gttctccacc
     1021 gaccagagct accaggtcca ggccaagttc gccaacatcg gcgggctcaa ggtgcgcggc
     1081 ccggtgaaaa gcgcgggcgt ggtcgtcgga cgcgtgaccg acgtccgctt cgataacgaa
     1141 agctacgagg cgatcgtgac gatgacgctc gaccacggat accagttccc gcgcgacacc
     1201 accgcgaaga tcctcacctc cggtatcctt ggcgagcaat acgtcgggct cgaggcgggc
     1261 ggggacggcg tcatgctgaa gaacggcgat cgccttcgtc tcacgcagtc ggcggtagtg
     1321 ctggaaaatc tcatcagcca gttcctgttc aacaaggcga ccgaagcaaa gccccaggac
     1381 aagtgacccc ggtaaggcat gcgaccctga cggcggctct gtgcctgctg ctctgctccg
     1441 gctgcgcagg cgtggagggc gagcgcgacc cgcgcgaccc gtgggaaggc ttgaaccgcg
     1501 gcgtgtaccg cttcaacgag acggtcgacg aatacgtcgc gcagcccctc gcgcgcgcct
     1561 atgtggcggt gctgcaccag gagatccgca cccgcgtgag caatttcttc gccaacatcc
     1621 aggatctcct catcggtgcc aacaatctcc tgcagggcaa gttcgaggac gggatcaccg
     1681 actgggcgcg cttcgtattc aacagcagct tcggcctgtt cggcctgcac gacttcgcct
     1741 ccgatgcagg cctggagaag cacaacgagg acttcggcca gaccctcggc cgctggggcg
     1801 cgggcccggg gccctacctc gtgctgccgt tcctgggctc gagcacggtg cgcgacgcgg
     1861 ccggcacggc ggtcgactgg gcggtgcagc cggtcggcga agtgcgcccc atcgcgctgc
     1921 gcaacagcct ttacgggttg tacttcgtgg acacgcgcgc gcagcttctc gacgcgagcc
     1981 gcatcctcga ggaggcggcc ctcgacaagt acgtgttcca gcgcgatgct tatctgcaac
     2041 ggcgccgcag cctgatctac gacggcaggc cgccgcggga gaaaatcgaa tgacgcggat
     2101 gctgctgaag tttgcgctcg tcgcttttgc cgcagcgggg tcgccgcttg gcgcgcagga
     2161 actcgcgccc gacgcgctgg tgaagaacgt cacgctggag gtcgtggaga tgatctccaa
     2221 ggacaaggag atcaaggcgg gcagccgcgc gaagctggtc gcgctcgtgg aggagaaagt
     2281 gctgccgcat ttcaatttca cggctatgac cgcgcttgcc atggggcaga actgggccaa
     2341 ggcgacgccc gagcagaaga agcagctgac cgagcagttc aggacgctgc tcgtgcgcac
     2401 ctacgcgagc gcgctcgcgg cctacagcga ccagaagttc gatttccgcc cgctgcgcgc
     2461 gaagcccacc gacacggatg tcacggtcaa cgtccgcgtc ctgcagcccg gagcgcagcc
     2521 ggtaacgctc gactacagca tggaaaagac ggcgaacggc tggaaggtct acgacgtgat
     2581 ggtagccggc gtgagcctgg tcgcgaacta ccgcaccgag ttcgccaaca ccgtgcgcgc
     2641 ttccggggtg gacgcgctga tccgcgattt gcagaccaag aacaagtcta tggagaagaa
     2701 gtgatccggc gcgaaggacg ccgcatcacc ctcagcggcc cggtcacgct cgcgaatgcc
     2761 gcacagctcc tcgagcaggg ccgccagcac ttcgccgaag gcgcacgcac cgtggatctc
     2821 ggcgaagtga cggagctcga ctcggcgctg cttgcgctgg tgctccaatg gctgcgcgag
     2881 gcgcgcgccg caaaacgcga gctcgcgctg gcgaaccttc cgcaatcgct cgatacgctc
     2941 gcgcgcctct acggcgtgca agacctgctt cccgtggccc gctgaaggcg atcgaagtcc
     3001 gcggtgtcga caagcgctac ggcacgctgc gcgcgctgtc ggaggtgagc ctcgccatcg
     3061 acacgggcga gtttttcgga ctgctcggcc cgaatggcgc cggcaagacc acgctgatca
     3121 atgtgatcgc cggtctcgcg cggcccgacg ccgggtcggc ctcggtgatg ggcgccgacg
     3181 tggcgcgcga ctatcggcgt gcgcgccgca tgctcggcgt ggtgccgcag gagctcgttt
     3241 tcgacccgtt tttcaccgtg cgcgagacgc tgcgcatcca ggcggggtac ttcggaatcc
     3301 gcggcaacga cgcgtggatc gaggaagtca tgcaccacct cgacctcgcg gaaaaggcgc
     3361 aggcgaacat gcgatcgctg tccggcggca tgaagcgccg cgtgctggtg gcgcaggctt
     3421 tggtgcacaa gccgccggtc atcgtgctcg acgagccgac cgcgggcgtg gacgtggagc
     3481 tgcgccaggg gctgtgggac ttcgtgcggc gcttgaaccg cgacgggcac accatcgtgc
     3541 tgaccaccca ctacctcgag gaggcggaaa cgcattgcag ccgaatcgcg gtgctcaagg
     3601 cgggccgtgt cgtggcgctc gactccaccc gcaacctgct cgccgcgtcg tcaggctcgc
     3661 gcatcagcgc acgggtggtg gggcgcgacg gcagacagca tctgctgcag atcgagggtt
     3721 atgcccagct cgagcgcgag ctcgcgcgtc tgcgcgagag cggcgtcgag atcgctgacc
     3781 tggagatcga gcgcccggat ctggagcagg tcttcctgcg gctcaccgcc gaatgagctt
     3841 ctggacgctc ttcaccaagg agctgttgcg cttcctcaag gtcagcgtcc agacggtggc
     3901 cgcgcccgtc ctcacctcgc tcctgtacct gatgatcttc ggccaggtgc tcgagggccg
     3961 gctccgggtc ttcgaggccg tgccctatac ctcgttcctc gtgccggggc tcgtgatgat
     4021 gagcgtcctg cagaacgcct tcgccaacag ctcgtccagc ctgatccagt ccaagatcac
     4081 gggcaacatc gtcttcgtcc tgctcgcccc gctttcccac gccgagttct tctgggcgta
     4141 cgtcgcggct gccgtggcgc gcggcctcgc cgtcggcgcc ggcgtcctca cctgcacatc
     4201 agcgttcgtc gaactggagc tccaggcgcc ggtctgggcg gcagccttcg cggttctcgg
     4261 cggagccctc ctcggcgccc tcgggctcct tgccggcatc gtttcggaga aaatagacca
     4321 gctcgccgcg ttccagaact tcgtcatcct gccccttacc atgctctcgg gcgtgttcta
     4381 ctccatccac tcgctgcccg gcttctggcg cgaagtttcg caggcgaatc ccttctttta
     4441 catgattgac ggcttccgct acggcttctt cgcggcgtcg gatgtcgcgc ccgggatcag
     4501 cctggcagta gtcgcggtcg cattcgtcgc ggtggcgtgg gtatgcctgc ggctgctcaa
     4561 atccggctac cggctgagga catagatggt gactgctgac agcgtaaagc gcggcatcga
     4621 gtcgggcctt gcctgcgagc acgtcgaggt ggtcggggac ggccagcatt tccaggcgct
     4681 cgtcgtgtcg gccgagtttg ccggccgtaa ccgggtgcag cgccaccagc tcgtctatgc
     4741 ggcgctcggc gatcgcatgc gcgaggagat ccacgctctg tcgatgcaga cgctgacgcc
     4801 ggacgaatgg aaaggccgtg gataagctca gaatcatcgg cggaaagccg ctcgaaggcg
     4861 agatccggat ctccggcgcg aagaacgccg ccttgccgat catgtgcgcc gccctgctta
     4921 cggcgaagcc gttgaccttg accaatgtcc cggcgctgcg cgacgtgtcg accatggcca
     4981 agctcctcgg gcaaatgggc ctgcgggtcg atcgctcccc tgcacgggtc gcgctgcatg
     5041 ccgcgagcat tgaggatccg acggcgtcct atgacctggt gaagaccatg cgcgcctcgg
     5101 tgctggtcct tggcccgctg cttgcccgct gcggccgcgc caaggtgtcg ctgccggggg
     5161 gctgcgccat cggcgcccgt cctgtcgatc agcacgtcaa ggggctggag gcgatgggcg
     5221 ccacgatcgc catcgagcat ggctacatgc atgccaccgc aaagcgcttg cgcggggcgc
     5281 gcatcgtgat ggaccttgtc acggtcaccg ggaccgagaa cttgatgatg gcggccgcgc
     5341 tcgccgaggg cgagacgctg atcgagaacg cggcgcgcga gcccgaagtg gtggacctcg
     5401 cccgctgtct caccgcgatg ggggcgaaga tcgagggcgc aggcagcgcc gtgattcgca
     5461 tcaccggcgt cggctcgctc gacggcgccg agcaccgcgt catgcctgac cgcatcgaga
     5521 ccggcacgta tctcgccgcg gcgacggcca gcggcgggaa cgtgcggctt ataggggccg
     5581 cgcccgattc gctcgatgcc acgctcgaca agctgcgcga ggccggggcg agaattgcga
     5641 cgagcgccga ttcgatcgag atccggatgt cggagcggcc aaacgcggtc agcgtgcgca
     5701 ccgcccctta ccccggattc gccaccgaca tgcaggcgca attcatggcg ctcgcaaccg
     5761 tggcgcgcgg cacggcggtg atcaccgaaa ccattttcga aaaccgcttc atgcacgcgc
     5821 tcgagatgca gcgcctcggc gctgacatcc ggctcgaagg caacaccgcc atcgtaaacg
     5881 gcgtcgagcg cctgcagggg gcgcgcgtca tggcgaccga cttgcgcgcc tccgcgggcc
     5941 tcgtcatagc cgggctggtg gccaccggcg aaaccctggt cgaccggatc taccacctgg
     6001 accgcggcta tgaggcgctg gagaccaagc tctcggcgct cggcgcgcgg gtggagcgcg
     6061 tgcgctaggg attgctgtag aatgcgcccc cctggcacgc atcacaaggc gcttccccgg
     6121 atggttgcac atggcgagca tcagcatcgc gttgtcgaag gggcggatct tcgacgagac
     6181 cgcgccgttg ctcgcgcgcg ccggcatcag gcccaaggct gacccggatt cttcgcgcaa
     6241 gctgatcatc ggcaccaacc ggcgcaatgt gcgactggtt gtggtgcggg cgaccgatac
     6301 tccaacctac gtgcagcatg gtggcgccga cctcggtatc gcggggcggg acgttctcgc
     6361 cgagcacggc gccgaggggt tgtaccagcc ggtcgacctc ggcatcgccg cctgccgcat
     6421 gatggtcgcc gtgccgaggg gcttcgacta tgcctcggct gtgcgccagg gcgcgcgcct
     6481 gcgcgtggcg accaagtacg tgaacaccgc gcgcgcgcac ttcgccgcca agggcgtgca
     6541 tgtggatctc atccggctgt acggctcgat ggagctggca ccgctcgtcg gcctggccga
     6601 cgcgatcgtc gatctcgtct ctacggggag aacgctgaag gcgaacgggc tcgtcgccgt
     6661 cgaagagctc atgccggtgt ccgcgcggct gatcgtcaac caggcggcgc tcaagaccaa
     6721 gcgcgcagag ttgcagccgc tcatcgacgc ttttgcacga gcggctcgcc ggtgaagctg
     6781 cgccgcctat cgacgcgcgc acgcggcttc gaagcgaagc tcgccgcgct cacgcgctac
     6841 gaggcggcac aagacccggc gctgcagaag gtggtgcgca aaatactcgc ggacgtacga
     6901 aagcgcggtg acgcagcagt tcgcacctac acgcggaagt tcgacggcgt ggcgcccggt
     6961 caatacgccg tatccgacaa actcctcgat gccatcccgg ccgatcaggc gcaggcgctg
     7021 cgcgcggcgc acgagcgcat ccgcgccttc catgaaaagc agctgcagca atcgtggcac
     7081 ttcaccgacg ccgacggcac ccgcctcggc cagcaggtaa gcccgctcca gcgggttggg
     7141 ttgtacgttc cgggggggaa agcagcgtat ccttcatcgg ttctgatgaa cgccgtgccg
     7201 gcgaaggtcg cgggcgtggg cgagctcatc atggcaagcc ccaacccgag cgcgctggtg
     7261 ctcgccgctg cagcactcgc cggagttgac cgcgtgcttg gcatcggcgg cgcgcaggcg
     7321 atcgcggcgc tcgcttatgg aacgcagtcg atcccgcgcg tggacaagat cgtcggtccg
     7381 ggcaacgcct acgtcgcgga agcgaagcgg caggtgttcg gcgaggtagg catcgatatg
     7441 gtggccggtc cctccgagat cctgatcatc gctgacgcct cggtaaatcc ggactggatc
     7501 gcgatggatc ttttctccca ggcggagcac gacgagtcgg ctcaggccat tctcctttgc
     7561 cccgacgaga agtatctcga ggcggtcgcc ggttccatcg ccaagctgat cgccgaaatg
     7621 ccgaggagga aggtcatcga ggcatcgctc aagtcgcgcg gcgccctgat acggacgcgc
     7681 gacctggcag aagcatgcgc agtggcgaat cgcatcgcac ccgagcacct ggagctcgcg
     7741 gtgcaagacc ccgaaaagat tcttgcttcc gtgagaaccg cgggggctgt cttcctaggg
     7801 cagtggagct ccgaagcgat cggcgactac tgcgcgggcc cgaaccacgt gctccccacc
     7861 gcgggcacgg cgcgcttctc ctcgccgctc ggcgtctacg atttccagaa gcggaccagc
     7921 gtgatcggcg tgtccaagca ggcagcggcc aagctcggtc gagtggccgt cacgctggcc
     7981 gagggcgaag gcttgagcgc gcacgccagg gccgcgcaaa tgaggttcga gtgactccgg
     8041 aacagctcgt tcgcgcagag atcctcgcgc tcaaggccta ccacgtcgcg cccgccgagg
     8101 ggatggtgaa gctcgacgcg atggaaaacc cctaccccct tcctccggcc gtgcgccgcg
     8161 agctggccga ggcgctggcg cgggtggacc tcaatcgcta tccggacccg gccgcgccga
     8221 aactgcgcga gctcatcgca cgcaagatgg gtgttcctcc cgggatggaa ctgctgctcg
     8281 gcaacggctc ggacgatctc attcagatgg tgacgctctc actcgccagg ccgggcgccg
     8341 tcatgatgta tccggccccc accttcgtca tgtacgcgat gaacgccact tacttcggca
     8401 tgcggaccgt ggcgaccgga ttgacggagg acttttcact cgacgccgcg gccttcatcc
     8461 gggccatgcg caaagagaac ccggccctca tctatatcgc gtatcccaac aacccgaccg
     8521 gtgtgctcta tgccgaggat gacatcgtcg cgatacttaa cgaagcacca gggctcgtcg
     8581 tgctcgacga ggcgtatcac gtattcgcgc gcaagacctt gatgccgagg cttgtgcaat
     8641 tttcgaacct cgtcgtcatg cgcacggtgt ccaagctggg gctcgccggg atccggctcg
     8701 ggtatctcgc cgcccgtccg caatgggtgg agcagttcaa caaggtgcgc caggtctaca
     8761 acgtcaacgt gctgacgcag acggcggcgg ctttcatgct cgagcgcatc gaggtcctcg
     8821 aagagcaggc ggcgaagatc cgggccgaga gagaaaagat gggccagtca ctaaaggcgt
     8881 tgcacggcgt gacggtattc ccatccgaag cgaacttctt cctggtccgc gtcccggacg
     8941 cggaccggac gttcgaaggg ttgaagcgcc agggcgtgct ggtgcgcaac ctgaatagcc
     9001 cggcgctgaa gaactgcctg cgcattacgg tcggcacgcc cgacgaaaac cgtatactgc
     9061 tcaacgccct gaaggaagca ttgcaatgag ggcgctgcaa tgaggaccgc cgaagtcacc
     9121 cgcactacca aggaaaccca gatccgggtg aagctcaacc tggatggcaa gggcacggca
     9181 cgcctgtcca cggggctgcc tttcctcgag cacatgctcg accaggtcgc gcgccatggc
     9241 atgctcgacc tcgacatcga ggcgaagggc gatctccata tcgacgcgca ccacaccgtc
     9301 gaggacatcg ggatcacgct cggccaggcc ctcgccaagg cgatcggcga caaggccggc
     9361 gtgcggcgct tcggccacgc ttacgtaccg ctcgacgaag cactgtcgcg ggtggtgctc
     9421 gacttctccg ggcggcccgg gctcgagtat cacgtgaagt tcacgcgcgc gctgatcggc
     9481 gagttcgacg tggatctggt gcacgagttc ttccagggat tcgtcaacca tgcccagctc
     9541 accctgcaca tcgacaacct gcgcggcgac aacgcacacc accagtgcga gacgatgttc
     9601 aaggcgttcg cgcgcgcgct gcgcatggcg gccgagcccg atccgcgcgc tgcgggcagc
     9661 gtgccctcca ccaagg
//
LOCUS       DASUNT010000214         2800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_554494, whole genome shotgun sequence.
ACCESSION   DASUNT010000214 DASUNT010000000
VERSION     DASUNT010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554494"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..257)
                     /locus_tag="VFX94_04250"
     CDS             complement(3..257)
                     /locus_tag="VFX94_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767427.1"
                     /translation="MALPLIKLGLAAAAGYYFYNRFRRSRGAGSTTNASDAVDDVKLG
                     PNPTVEEKLDAGVQETFPASDPVSVAHPSETAYEQKQRSS"
     gene            complement(301..489)
                     /locus_tag="VFX94_04255"
     CDS             complement(301..489)
                     /locus_tag="VFX94_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767428.1"
                     /translation="MAPSSVRRSAGSHTNAAGAIDDIELGPDASADDVLDEGLRETFP
                     ASDPVAVSAAYERKLRRG"
     gene            714..1553
                     /locus_tag="VFX94_04260"
     CDS             714..1553
                     /locus_tag="VFX94_04260"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5767429.1"
                     /translation="MNVGFLGLGQMGRPMVERLKGAGHAVKAYNRTRLSADACETPDA
                     VLDSDAVITMLADDAAVRAVWLDSGLARRLPRGAVHLNMATVSMRIARELAAIHGESY
                     AAAPVFGRPPLAARGELDIIAAGPPAALEKCEPLLRVLGKQVFVVGEEPAKANAVKIA
                     RNFLLAAMIESLGEAFALVRKCGVEPQRFLDVIGSTSLGSPACRNYGKLIVEQAWTPA
                     QFAMPLGVKDVELTLATAQDAEMDLETARLVRKNLLAARAAGRGEEDWAALAGHIAAE
                     AGL"
     gene            complement(1605..1952)
                     /locus_tag="VFX94_04265"
     CDS             complement(1605..1952)
                     /locus_tag="VFX94_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767430.1"
                     /translation="MMRRLAAALLLAGCAAPYTDAGTYEAVLPAAGGGDRHLRVSLDK
                     SGAAAMTSTFTSRPDGFLLEGTWRREGSRITVSFDKQDIVFRRSGTLLKAEQWDRSFW
                     GDIGPAVLERVNR"
     gene            complement(1962..>2800)
                     /locus_tag="VFX94_04270"
     CDS             complement(1962..>2800)
                     /locus_tag="VFX94_04270"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX5767431.1"
                     /translation="YHGLEFDTTGACVKVPSQERVPPSAKVRSYPVVERNHWIWLWTG
                     DPAKADPTLIEDFHWLEDPKWGYGGNYLHVEANYLLLVENLLDTTHLPFLHPDTLGTD
                     AFARSEFDVKREGDRITVTRWLMNELPAPFHKQMGGFPDGVKVDRWQITHFGPPCFVK
                     LDVGSAPAGAGARQGDRSNGMNMWNLNAITPETEKTAHYFFAQAYNFRLDEPWVANML
                     RTQVHTIFLEDMAMIKAQQENMDLGPSPSSVHLGQDKAWVAMRGIVDRLVREEQQQAR
                     AA"
BASE COUNT          432 a          902 c          981 g          485 t
ORIGIN      
        1 gcttaactag agcgctgttt ctgctcgtag gcggtttcgg aggggtgagc cacgctcacc
       61 gggtcgctgg ccgggaacgt ttcctgtacg cctgcgtcga gcttctcctc gaccgtggga
      121 ttgggaccga gcttgacatc gtccaccgcg tcgctcgcgt tcgtcgtcga gccggcgccg
      181 cgcgacctgc ggaagcggtt gtaaaagtaa tagcccgccg cggcggcgag cccgagcttg
      241 atcaatggaa gggccatggt atgtctcctt gaagaccctt ccttgcaacc cgcgtgcccg
      301 tcagccgcgc ctgagcttgc gctcgtacgc ggcgctgacc gcgacagggt cgctcgccgg
      361 aaaggtctcg cgcaggcctt cgtccagcac gtcgtcggcg ctggcgtccg gaccgagctc
      421 gatgtcatcg atggcgccgg ctgcgttggt atgcgagccg gcggaccggc gcacggagga
      481 gggggccatg gcgcagtcgt gagcatcttt ggtgccggca aaaaaaagcc gccgcagcta
      541 ccgccacggc ggccgaaaag ccgcgctaca gcccccaatt cttctttttc tccccgctgc
      601 agagcctgcg gccaggcccg cgcagcggca agtcaggctt tgtgaggcgc ttgaactact
      661 tcacccggga tacgatttcg ctcgcgcgag cgagaaagaa aggcggcggt tccatgaatg
      721 tcggttttct cgggctcggc cagatgggcc ggcccatggt ggagcgattg aagggtgcgg
      781 gtcacgcggt gaaggcctac aaccgcaccc gtctatcagc cgacgcgtgc gaaacccctg
      841 acgcggtgct cgacagtgat gccgtcatca cgatgctcgc cgacgacgcc gcggtgcgcg
      901 cggtgtggct tgactcgggg ctggcgaggc ggctgcctcg cggggcggtg cacctcaaca
      961 tggcgacggt cagcatgcgc atcgcgcgcg agctcgccgc catccacggg gaatcctatg
     1021 ccgcggcgcc agtgttcggc cggcccccgc tcgcggcgcg aggcgagctc gacatcatcg
     1081 ctgccgggcc gccggccgcc ctggagaagt gcgagccgct gttgcgcgtt ctgggcaagc
     1141 aggtgttcgt ggtgggcgaa gagccggcca aggcgaatgc cgtgaagatc gcgcgcaact
     1201 tcctgctcgc cgcgatgatc gagagcctgg gcgaggcgtt cgcgctcgtg cgcaagtgtg
     1261 gtgtcgagcc gcaaaggttc ctggacgtaa ttggcagcac gtcgctcggc tcgcccgcct
     1321 gcaggaacta cggcaagctg atcgtcgagc aggcgtggac cccggcgcag ttcgccatgc
     1381 cgctcggcgt caaggacgtg gagctcacgc ttgcgaccgc gcaggacgcg gaaatggacc
     1441 tggaaaccgc ccgcctggtg cgcaagaacc tccttgccgc cagggcggcc gggcggggcg
     1501 aggaggactg ggcggcgctc gcgggccata tcgccgcgga agcggggctg tgaccgacga
     1561 gtgtccgcgc gtggtgcgcg gacttcaatg cctgaacaag cggtctagcg atttacgcgc
     1621 tcgaggaccg cgggtcctat atcgccccaa aagctgcggt cccattgctc ggccttcagc
     1681 agggtgcccg aacgccggaa cacaatgtcc tgcttgtcga agctgacggt gatgcggctg
     1741 ccttcgcgcc gccaggttcc ctcgaggagg aaaccgtccg ggcggctggt gaacgtgctc
     1801 gtcatggcgg cggcgccgct cttgtcgagc gagacgcgca gatgccggtc tccgccgccg
     1861 gcggccggaa gcaccgcctc gtaggttcct gcgtcggtgt agggggcggc gcagcctgcg
     1921 agaagaagtg cggcggcaag gcgccgcatc atccggtgcg ttcaggccgc acgcgcctgc
     1981 tgctgctctt ccctgaccag gcgatccacg atgccgcgca tcgccaccca ggccttgtcc
     2041 tggcccaggt ggaccgatga cgggctgggc ccgaggtcca tgttttcctg ctgcgccttg
     2101 atcatcgcca tgtcctcgag aaagatcgtg tgcacctggg tgcgcagcat gttcgcgacc
     2161 cagggctcgt cgagcctgaa gttgtaggcc tgggcaaaga agtagtgcgc ggtcttctcg
     2221 gtctccggcg tgatcgcgtt caggttccac atgttcatcc cgttcgagcg gtcgccctgg
     2281 cgggcgccgg cgccggccgg cgccgagccg acgtcgagct tgacgaagca cggcggcccg
     2341 aaatgggtga tctgccagcg gtcgaccttc acgccatcgg ggaatccgcc catctgcttg
     2401 tggaacggcg cgggcagctc gttcatgagc cagcgcgtga cggtgatgcg gtcgccttcg
     2461 cgcttcacgt cgaactcgga gcgcgcgaac gcgtccgtgc cgagcgtgtc cggatgcagg
     2521 aaggggaggt gggtggtgtc gagcaggttc tccaccaaga gcaggtaatt cgcctccacg
     2581 tgcaggtaat ttccgccata gccccatttg gggtcttcga gccagtggaa gtcctcgatg
     2641 agggtcgggt cggccttcgc cgggtcgccg gtccagagcc agatccagtg gttgcgctcc
     2701 accaccgggt aggacctgac cttcgccgaa ggcggcacgc gctcctggct cggcaccttg
     2761 acgcacgcgc cggtcgtatc gaactccagg ccgtggtagg
//
LOCUS       DASUNT010000215         3305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_292172, whole genome shotgun sequence.
ACCESSION   DASUNT010000215 DASUNT010000000
VERSION     DASUNT010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292172"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFX94_04275"
     CDS             complement(<1..452)
                     /locus_tag="VFX94_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEX5767432.1"
                     /translation="MRQYADFMARAYTMELEAIERYTQFAEQLETHNNREVAQLFRKL
                     AEIENLHAKRILQEMNWPSVPALPPAYAWDGDEGPETAPFDSLHYLMQPWHALEIALR
                     CERDAQQYYENIAASGAPRKVRDAAREMASEEAEHVRLIQEWMKRVPRP"
     gene            complement(449..1282)
                     /locus_tag="VFX94_04280"
     CDS             complement(449..1282)
                     /locus_tag="VFX94_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX5767433.1"
                     /translation="MNAPLAATTLQAKDFKSDYKPVWCPGCGDYSVLSAITKALALLE
                     LRPENVAVVSGIGCSSRIPAYTTCYGFHGVHGRSLPAATGLKVARPDLTVLVASGDGD
                     GYSIGGNHFLHACRRNVDLTYIVMDNHVYGMTKGQPSPTTEPDWSSKLAPGGTGLREF
                     HPLVIALASGANFIARAFSGDPNGCATLIAEAVRHPGFSFIEILSPCVTFRPEQRGWK
                     DVVHPAPVESTDDPGRAARRLMTDDGFNIGVLYKGKRPPYTAARDGSGRVEDLQKAFQ
                     L"
     gene            complement(1279..2943)
                     /locus_tag="VFX94_04285"
     CDS             complement(1279..2943)
                     /locus_tag="VFX94_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX5767434.1"
                     /translation="MRNSVSVALAGSGGAGVMTAGNMLLEAAAAAGYYGLMTRTSGPQ
                     IRGGEAAAMVRVTSFPTDAQENRFHALAAIDWQNVGRFAAEIPLGPESVVVGDPAHGA
                     PPEAFIKSGARYVPVPLKKLAGEIQGAWPNMVALGVLAGLIGLPRDAVEVAARKSMKK
                     GLEPGIAAARLGFSQVREFDGYRLSPRENPRCRWLISGNEAAGLGALRGGVRFVAAYP
                     ITPATELLEWLAPALTRVGGTLVQAEDELASINMALGASYGGVPALTATSGPGLSLMI
                     ESLGLGVAAEVPVVVVDVMRTGPSTGIATKTEQGDVAIALYGMHGDAPHIVVAPNSIS
                     DCIAATQWAVGLAEALQAPAIVLSDQYFGQARAIVDVPVLEVMNFERDKAKAGDGYKR
                     YALTPSGVSPMAIPGTAGCTYTADGLEHNERGTPSSQALDHLAQMDKRARKISQLDSG
                     DAWASIEGEGETAIVTFGSCTGPVREALLNRPFKLISLRLLSPAQPAKLKKALEGVKR
                     VLVVEQNHSGQFLRYLRAEYELPAEVKSLRRPGPLPFRPEEIQAAL"
     gene            3073..>3305
                     /locus_tag="VFX94_04290"
     CDS             3073..>3305
                     /locus_tag="VFX94_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767435.1"
                     /translation="MAAPETFFLRSHDLAEISVPGVRVERRPAREPDGTAAPGLHNAW
                     IWLDNPSQYHSDTTEMVKGVILAFRAASNARDV"
BASE COUNT          520 a         1107 c         1129 g          549 t
ORIGIN      
        1 gggcgcggca cgcgcttcat ccattcctgg atcaggcgca cgtgctcggc ttcctcgctc
       61 gccatctcgc gggcggcgtc gcgcaccttt ctcggcgcgc cgctcgcggc gatgttttca
      121 taatattgct gcgcgtcgcg ctcgcagcgc agcgcgatct cgagcgcgtg ccacggctgc
      181 atgaggtagt ggagcgagtc gaacggcgca gtctccgggc cctcgtcgcc atcccaggcg
      241 tacgccggcg gcagcgccgg cacgctgggc cagttcattt cctggagaat gcgcttcgcg
      301 tgcaggtttt cgatctcggc gagcttgcgg aacagctgcg ccacctcgcg gttgttgtgc
      361 gtctcgagct gctcggcgaa ctgcgtgtaa cgctcgatgg cctcgagttc catggtgtag
      421 gcccgcgcca tgaagtcggc gtactgcctc atagttggaa tgccttttgg agatcctcca
      481 cgcggccgct gccgtcccgc gcggctgtgt acggcggccg cttgcccttg tagagaacgc
      541 cgatgttgaa gccgtcgtcg gtcatgagcc gacgcgcggc gcggccgggg tcgtctgtgg
      601 attccaccgg cgccggatgg accacgtcct tccagccgcg ctgctcgggc cggaaggtga
      661 cgcaaggcga gaggatctcg atgaacgaga agcccggatg gcgcactgcc tcggcgatga
      721 gcgtcgcgca gccgttcggg tcgccggaaa acgcgcgcgc gatgaagttc gcccccgagg
      781 cgagcgcgat caccagcgga tggaactcgc gcaaaccggt gccgccaggc gcgagcttcg
      841 aggaccagtc gggctcggtg gtgggcgagg gctggccctt ggtcatgccg tagacgtggt
      901 tgtccatcac gatgtaggtc aggtcgacgt tgcgccggca cgcatggagg aagtggttgc
      961 cgccgatcga gtacccgtcg ccgtcgccgc ttgcgaccag caccgtgaga tcaggacgcg
     1021 ccactttcag cccggtggcc gcgggcagcg agcggccgtg cacgccgtgg aatccgtagc
     1081 aggtggtgta cgcgggaatg cgcgaggaac agccaatgcc tgacaccacg gcgacgtttt
     1141 cgggccgcag ctcgaggagc gcgagcgcct tggtgatcgc cgagagcacc gaatagtcgc
     1201 cgcagccggg acaccacacc ggcttgtagt cggatttgaa gtccttcgct tgcagcgtcg
     1261 tggcggcgag cggggcgttc atagggctgc ttggatctcc tcgggacgga aaggcagcgg
     1321 cccggggcgg cgcaggctct taacttcggc ggggagctca tattcggcac gcaggtagcg
     1381 caggaattgg ccgctgtggt tctgctcgac cacgagcacg cgcttcacgc cctcgagcgc
     1441 cttcttcagc tttgccggct gcgcgggcga aagcaggcgc agcgagatga gtttgaacgg
     1501 tctattcagc aaagcctccc gtaccggccc ggtacaggag ccaaatgtca ctatcgccgt
     1561 ctctccctcg ccctcgatgc tcgcccaggc gtcccccgag tcgagctgcg agattttgcg
     1621 cgcgcgcttg tccatctgcg ccaggtgatc gagcgcctgc gacgacggcg tgccgcgctc
     1681 gttgtgctcc aggccgtcgg cagtgtaggt gcatcccgcg gtgcccggaa tcgccatggg
     1741 ggacaccccg gagggcgtca gcgcatagcg cttgtagccg tcgccggctt tcgctttatc
     1801 tctttcgaaa ttcatgacct ccaaaaccgg aacgtcgacg atcgcgcgcg cctgaccgaa
     1861 gtactggtcg gagagcacga tcgccggggc ctgcagcgcc tccgcgaggc cgaccgccca
     1921 ttgcgttgcc gcgatgcagt cggagatgga attcggcgcg accacgatgt gcggggcgtc
     1981 gccgtgcatg ccgtagagcg caatggcgac gtcgccctgc tcggtcttgg tggcgatgcc
     2041 ggtagagggt ccggtgcgca tcacgtccac caccaccacc gggacctcgg cggcgacgcc
     2101 gagcccgagc gactcgatca tgagcgagag ccccgggccg gaggtcgcgg tgagcgccgg
     2161 cacgccacca taggacgcgc cgagcgccat gttgatcgag gcgagctcgt cctcggcctg
     2221 caccagcgtg ccgccgaccc gggtcagcgc gggcgccagc cattcgagca gctcggtcgc
     2281 cggagttatc gggtacgccg cgacgaagcg cacgccgccg cgcagcgcgc cgaggcccgc
     2341 ggcctcgttc ccgctgatca gccagcggca tctggggttc tcacgagggc tgagccggta
     2401 gccgtcgaac tcccgtacct gcgaaaaacc tagccgtgcc gccgcgatgc cgggctcgag
     2461 tcccttcttc atggacttgc gcgccgcgac ttcgaccgcg tcgcggggaa gcccgatcag
     2521 cccggcgagc acgccgagcg ccaccatgtt cggccaggcg ccctggattt caccggccag
     2581 cttcttcagc ggaaccggga cgtagcgcgc gccgcttttg atgaacgcct cgggcggggc
     2641 gccgtgggcg gggtcgccga cgacgacgct ctccgggccg agcggaatct cggcagcgaa
     2701 gcgccccacg ttctgccagt cgatcgccgc cagcgcatgg aaccggttct cctgcgcgtc
     2761 ggtagggaaa gaggtgacgc gcaccatcgc cgcagcctcg ccgccgcgga tctgcgggcc
     2821 gctggtgcgt gtcatgagcc cgtagtagcc ggccgccgcc gcagcttcca gcaacatatt
     2881 gccggcggtc atgacgccgg caccgccgct gcctgcgagc gctacggaga cggagttgcg
     2941 catgcgcgca atttcgccga accgcgcgcg caaaccttga gccatatcaa atccggcgcc
     3001 ttttcccggg ataaaaatcc caaagacccc aaatcggtat tcgaatgctt atttgagacg
     3061 aggagagatc ccatggcggc ccccgagacg ttcttcctga gaagccacga cctggcggag
     3121 atttcggttc cgggggtgcg ggtcgagcgc cgtccggcgc gcgagcccga cggcacggcg
     3181 gcgccgggcc tgcacaacgc ctggatctgg ctcgacaacc cctcgcagta ccactcggac
     3241 accaccgaga tggtgaaggg cgtgatcctc gctttccgcg ccgcgtcaaa cgcgcgcgac
     3301 gtgaa
//
LOCUS       DASUNT010000216         4732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_993015, whole genome shotgun sequence.
ACCESSION   DASUNT010000216 DASUNT010000000
VERSION     DASUNT010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993015"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VFX94_04295"
     CDS             <1..642
                     /locus_tag="VFX94_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5767436.1"
                     /translation="GNHMGEPVSMARAEQLLFGVCLLNDWSARDIQAWEYQPLGPFLA
                     KSFATTISPWIVTLEALAPFRCPAYARATGDPKPLSYLWDDEDQRTGGLDIQIEMHLK
                     TRKMHAPVRLSRASFRESYWTVAQLVAHHASNGCNLRPGDLLGSGTISGATLDSYGSM
                     MELTQGGKNPLTLPDGEMRMFLEDGDLVVQKGYCESATHARIGFGEAAGAIRP"
     gene            639..1175
                     /locus_tag="VFX94_04300"
     CDS             639..1175
                     /locus_tag="VFX94_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEX5767437.1"
                     /translation="MSWRVAALLPALACAGLLGFGYYLQYVQGLQPCPLCLVQRGFFY
                     AVAGTCVIAAIHGPSAWGRSVYGIFAALFAAGGFATAARQVWLQHLPPERVPACGPDL
                     YFMLDNFPLARVVKTLIQGTGECAVVDWTFLGFSIAEWSLACFAGLFFYALWLAVRRP
                     KAGSAADMRFPEVEGRYR"
     gene            complement(1088..1942)
                     /gene="cobA"
                     /locus_tag="VFX94_04305"
     CDS             complement(1088..1942)
                     /gene="cobA"
                     /locus_tag="VFX94_04305"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012212882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX5767438.1"
                     /translation="MCKTGVRPQPRKSGSNPDFPGTVYLVGAGPGAPDLLTLRAVRLL
                     AEADVVFHDALVHPETLALASRAQKIAVGKRCGRHSTAQQFINKRLADAARRDRVVVR
                     LKGGDPMLFGRAQEEIEFLKGRGIRVEIVPGVTAALAASAELGVSLTQRLVSRSVVFA
                     TPRTATGAERSGWEDAVAAADTAVLYMAAGEAAAVKEALLRRGMSAGTPVAIAENVSL
                     ESSNLRCGSLERLPELAATLQGGPALVLIGEVFAALQAQRYRPSTSGNLMSAAEPAFG
                     LRTASQSA"
     gene            complement(1896..2297)
                     /locus_tag="VFX94_04310"
     CDS             complement(1896..2297)
                     /locus_tag="VFX94_04310"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEX5767439.1"
                     /translation="MATLIKGRRVVAESQSSPKDKVLRLEPGDDPASVSLEGFARVEV
                     NFPKFGDGRGYSIARLLRERRAYRGELRAVGHITRDLLLFLERCGFDAFELREGEDAH
                     EALAAFDDFSHSYQRGYLQNRGQTPTEKIGL"
     gene            complement(2242..3915)
                     /locus_tag="VFX94_04315"
     CDS             complement(2242..3915)
                     /locus_tag="VFX94_04315"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX5767440.1"
                     /translation="MYRYDAIDQTLVDERVAQFRDQTRRFLAGELSEEEFRALRLRNG
                     LYIQRYAPMLRIAIPYGLLSTQQLRKLADLARRYDRGYAHFSTRQNVQLNWPRLEQVP
                     DILAELATVQMHAIQTSGNSFRNVTTDHFAGVAADEIVDSFVWCELLRQWSTFHPEFS
                     FLPRKFKIAVNGAAADRAAVGVHDIGLHATKNADHIGFRVLVGGGLGRTPMIGVTIRE
                     FLPWPHLLTYLEAILRVYNRYGRRDNIHKARIKILVKERGVERFRQEVEAEWAHLKDG
                     PATVTAEEIARIERRFTRPRYESGSDPDSSQPGFLAWRKRNVHPHKVPGYSAVTLSLK
                     KTGVPPGDATSEQMDAIANLADRYSFGEARVSHEQNLVLADVRSRDLPALWREARELG
                     LATPNIGLLTNIITCPGGDYCALANAKSIPVAQAIQERFEDLDYLHEIGELDLNISGC
                     INACGHHHVGHIGILGVDKNGEEWYQVELGGNQAPASIGKIIGPAFARAEVPDVVERL
                     IGCYLAHRDSEAERFVDVVHRIGIEPFKEHIYGHAHQGAARRSREPVLA"
     gene            3973..>4732
                     /locus_tag="VFX94_04320"
     CDS             3973..>4732
                     /locus_tag="VFX94_04320"
                     /inference="COORDINATES: protein motif:HMM:NF009327.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEX5767441.1"
                     /translation="MNLRHMKLQQLRCLTEVARRSLNVSEAAAALHTSQPGVSKQIRA
                     LEDELGIEVFVRHGKRLVAVTEPGRAVVAIAERILAEARNLRRAGEEFANDQIGTLTI
                     AATHTQARYALPKAVAGFKRRYPKVELLIHQGNPTQICDQVLAGEADMAVATEMISTY
                     PELISLPVYQWNRCVVVPPKHPLLKENALTLEKVAEYPIVTYDFAFANRSLVQKAFES
                     RGLKPHVVLSAQDSDVIKTYVELGLGVGILAKMAF"
BASE COUNT          779 a         1573 c         1577 g          803 t
ORIGIN      
        1 gggaatcaca tgggcgaacc cgtgtcgatg gcccgcgccg agcagctcct gttcggggtg
       61 tgcctgctga acgactggtc ggcgcgcgac atccaggcct gggaatacca gccgctcggc
      121 cccttcctcg cgaagagctt cgccaccacg atttcgcctt ggatcgtcac gctcgaagcg
      181 ctcgcgccgt ttcgctgccc ggcatatgcg cgcgcaaccg gggacccgaa accgctgtcc
      241 tacctctggg acgacgaaga ccagcgtacg ggcgggttgg acatccagat cgagatgcat
      301 ctgaagacgc gcaagatgca tgcgccggta cgcctgtcgc gcgcaagctt ccgcgagtcg
      361 tactggaccg tggcgcagct cgtggcgcac catgcgtcca atggatgtaa cctgcgccct
      421 ggggacctgc tcggctcggg aaccatttcg ggcgcgaccc tggactctta cggctcgatg
      481 atggagctca cgcagggcgg caagaatccg ctcaccctgc ccgacgggga aatgcggatg
      541 tttctggagg atggagacct ggtggtgcaa aagggctatt gcgaaagcgc gacacacgcg
      601 cgcatcggat tcggcgaggc ggcgggggcg atccggccat gagctggcga gtcgccgctc
      661 tcctgcctgc gcttgcttgc gcgggtctcc tcggcttcgg ctattacctg cagtacgtcc
      721 agggtctgca gccctgcccg ctatgcctcg tgcagcgcgg ctttttttat gcggtggcgg
      781 gcacctgcgt gatcgccgcg atccacgggc cgagcgcgtg gggcagaagc gtctacggaa
      841 tcttcgccgc cctgtttgcc gccggtggct tcgccaccgc ggcgcggcag gtgtggctgc
      901 agcatcttcc gcccgagcgc gtgcctgcgt gcggccccga cctctatttc atgctcgaca
      961 acttcccgct tgcgcgcgtg gtgaagacgc tcatccaggg caccggcgag tgcgcggtcg
     1021 tggactggac gtttctcggg ttctcgatcg ccgagtggtc gctcgcgtgc ttcgccgggc
     1081 ttttcttcta cgcgctctgg cttgccgtgc gcagaccgaa ggcgggctcg gccgcagaca
     1141 tgagattccc ggaggtcgaa ggccgatacc gctgagcttg gagcgccgcg aacacttcgc
     1201 cgatcagcac gagggccgga ccaccttgga gtgtggcggc aagctcgggc aagcgctcca
     1261 ggctcccgca acggagattc gaagactcga ggctcacgtt ctcggcgatc gccaccggcg
     1321 tcccggcgct catgcctcga cgcagcagcg cttccttgac cgcggccgct tcgcccgccg
     1381 ccatgtacag caccgcggtg tcggccgcgg cgaccgcatc ttcccagccc gaacgctcgg
     1441 cgccggtagc ggtgcgcggc gttgcgaaca cgacgctgcg cgacacgagc cgttgcgtga
     1501 gcgacacgcc gagctcggcg ctcgccgcaa gcgccgcagt gacgcccggc acgatctcga
     1561 cccggatccc tctgcccttc aggaactcga tctcttcctg cgcgcggccg aagagcatcg
     1621 ggtccccgcc cttcagccgc accaccacgc ggtcgcgtcg cgccgcgtcg gcaaggcgct
     1681 tgttgatgaa ctgctgcgcc gtcgaatgcc ggccgcaacg cttgccgacg gcgatcttct
     1741 gcgcccggga cgccagggcc agcgtttccg ggtgaaccaa cgcgtcgtga aaaaccacgt
     1801 cggcttcggc gaggagccgc acggcgcgca gcgtgagaag gtcgggcgcg ccgggacccg
     1861 cgccgacgag atagacggtg cctggaaaat cggggttaga gcccgatttt ctcggttggg
     1921 gtctgacccc ggttttgcag atatccacgt tgatacgaat ggctgaagtc atcgaacgcg
     1981 gcgagcgcct cgtgcgcgtc ctcgccctcg cgcagctcga acgcgtcgaa gccgcagcgc
     2041 tcgagaaaca gaagcaggtc gcgcgtgatg tgccccaccg cgcgcagctc gccgcggtaa
     2101 gcgcggcgct cgcgcaggag ccgcgcgatc gagtagccgc ggccgtcgcc gaacttgggg
     2161 aaattcactt ccacgcgcgc gaagccctcg agggagacag acgccgggtc gtcgcccggc
     2221 tccagacgca gcactttgtc cttaggcgag gactggctct cggctacgac gcgccgcccc
     2281 ttgatgagcg tggccataaa tgtgctcctt gaaaggttcg atcccgatgc gatgcaccac
     2341 gtcgacgaag cgctcggctt cgctgtcgcg gtgtgcgaga tagcagccga tcagccgctc
     2401 gacaacgtcg ggcacttccg cccgcgcaaa cgcgggcccg atgatctttc cgatggacgc
     2461 gggagcctgg ttaccgccga gctcgacctg gtaccactcc tcgccgttct tgtccacgcc
     2521 gaggataccg atgtggccga cgtggtggtg gccgcacgca ttgatgcaac cggagatgtt
     2581 gagatcgagc tcgccgatct cgtgcaggta gtcgaggtct tcgaagcgct cctggatcgc
     2641 ctgcgccacc ggaatcgact tggcgttggc gagcgcgcaa tagtcgcccc ccgggcaggt
     2701 gatgatgttc gtgagaagcc cgatgttcgg cgtcgcgagc ccgagctcgc gcgcctcgcg
     2761 ccacagcgcc ggcaggtcgc ggctgcgcac atcggcgagc accaggttct gctcgtgcga
     2821 cacgcgagcc tcgccgaaag agtaccgatc ggcgagattc gcgatcgcgt ccatctgctc
     2881 gctcgtcgcg tcgcccggcg gcacgccggt cttcttgagc gacagcgtca ccgccgagta
     2941 accgggcact ttgtgcgggt ggacgttgcg ctttctccag gcgaggaacc ccggctgtga
     3001 agaatcgggg tcagaccccg attcgtaccg tggacgggtg aagcgccgct cgatacgcgc
     3061 gatctcttcc gcagtgacgg tcgccgggcc gtccttgagg tgcgcccact cggcctcgac
     3121 ctcttggcgg aagcgctcga cgccgcgctc cttgaccagg atcttgatgc gcgccttgtg
     3181 gatgttgtcc cgccggccat agcggttgta gacgcgcagg atcgcctcga ggtaggtgag
     3241 cagatgcggc caggggagga actcgcggat cgtcacgccg atcatcggcg tgcgcccgag
     3301 cccgccgccg accagcaccc gaaagccgat gtggtctgcg ttcttggtgg catgcaaacc
     3361 aatgtcgtga acgccgacgg cggcccggtc cgcggcggcg ccgttcaccg cgatcttgaa
     3421 cttgcgcggc aggaacgaga actcgggatg gaaggtggac cactgccgca ggagctcgca
     3481 ccacacgaac gagtcgacaa tctcgtcggc cgcgacgccg gcgaaatggt cggtggtgac
     3541 gttgcggaag gagttgcccg aagtctggat cgcgtgcatc tgcaccgtgg cgagctcggc
     3601 caggatgtcg ggcacctgtt ccagccgcgg ccagttgagc tgcacgttct gccgggtcga
     3661 gaagtgggca tagccgcggt cgtagcggcg cgcgaggtcg gccagctttc tcaattgctg
     3721 cgtcgacaga agaccgtaag ggatcgcgat gcgcagcatc ggcgcgtagc gctggatgta
     3781 gaggccgttc cgaagcctga gcgcgcggaa ctcctcttcg gaaagctcgc ccgcaaggaa
     3841 gcgccgcgtc tggtcgcgga actgcgctac ccgctcgtcc accagcgtct ggtcgatcgc
     3901 gtcgtaccgg tacacggcgc cgattctcaa gcgggacctt attcctgaca aagactattg
     3961 tgttaggatg ttatgaacct gcggcatatg aagctccagc aactgcgctg cctgaccgag
     4021 gtcgcccggc ggagtctcaa cgtgtcggaa gcggccgcgg cgctgcacac ctcccagcca
     4081 ggggtatcga agcagatccg cgcgctcgag gacgagctcg ggatcgaagt cttcgtgcgc
     4141 cacggcaagc gcctggtcgc ggtcaccgag cccggacgcg cggtggtcgc gattgccgag
     4201 cgcatcctcg ccgaggcgcg caacctgcgc cgcgcgggcg aagagttcgc gaacgaccag
     4261 atcggcaccc tcaccatcgc cgcaacgcat acccaggcgc gctacgccct gccgaaggcc
     4321 gtggccgggt tcaagcggcg ctacccgaaa gtcgagctcc tcatccacca gggcaacccg
     4381 acgcagatct gcgaccaggt gctcgccggc gaggcggaca tggcggtcgc gaccgagatg
     4441 atctcgacct accccgagct gatctcgcta ccggtgtacc agtggaaccg ctgcgtcgtg
     4501 gtgcccccga agcacccgct cctcaaggag aacgccctca ccctggagaa ggtggcggaa
     4561 tatccgatcg tcacctacga ctttgcattc gcgaaccgct cgctcgtgca gaaggcgttc
     4621 gaaagccgcg gcctgaagcc gcacgttgtg ctgagcgcgc aggattcgga cgtcatcaag
     4681 acctatgtcg agctcggcct gggggtgggt atcctcgcga agatggcttt cg
//
LOCUS       DASUNT010000217         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_993020, whole genome shotgun sequence.
ACCESSION   DASUNT010000217 DASUNT010000000
VERSION     DASUNT010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993020"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFX94_04325"
     CDS             complement(<1..272)
                     /locus_tag="VFX94_04325"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012218382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX5767442.1"
                     /translation="MRSFVYNGLPARVVFGAGALARLPEELDRLGARRALVLSTPEQA
                     DSARQVANALGARCAGTYDKAAMHVPLEIAEDARHAARQLGADCCVT"
     gene            complement(280..1227)
                     /locus_tag="VFX94_04330"
     CDS             complement(280..1227)
                     /locus_tag="VFX94_04330"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5767443.1"
                     /translation="MRKLLAALLVAVPCFASAQSYPAKPVRIIVPFPPGGTTDLIARI
                     VQPRFQEFMGQTVLIENRSGAGGSVGAAEVARAPADGYTLLMVFDTHAVNHHIFKMAP
                     DPFKQLEHICLMVRSPSALVGVTSFAPNNLREVVAYAKANPEKVTYSTPGSGSSNHLA
                     ALLLEQVAGIKMTHVAYKGGGPMIQALLSQQVHISFFSLPLMVPHIKAGKMKGLAVGS
                     RARVPQLPDVQTLSETFPGFEQYSWFGILGPAGLPKDVAAKVHRETVRTLQAPDVNGK
                     LTEQGFEIVASSPEEFVKFVQGESDKLGRLIRDNKIVAE"
     gene            1264..1662
                     /locus_tag="VFX94_04335"
     CDS             1264..1662
                     /locus_tag="VFX94_04335"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5767444.1"
                     /translation="MKKQQIRTSKLAEPSGHFSQAIAAPAKGVLVFVSGMTARRPDGK
                     VVGVGDVEEQTRQVCENLKAALEAAGGTLDDVCRVDVYLRNIGDRERVNKVRRQFWRG
                     TPPASTLVEVSKLASPEFLVEINAIALVSP"
     gene            complement(1634..2179)
                     /locus_tag="VFX94_04340"
     CDS             complement(1634..2179)
                     /locus_tag="VFX94_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5767445.1"
                     /translation="MTSVPEFSFSHMGIFVTDPARMEDFYTRVLGFAVTDRGMLGSVS
                     LVFLSRDAREHHQIVLAAGRPAAAGFNPINQISFRMADFAGLREMHRRLEEEGVRELS
                     PASHGNALSVYFKDPEGNRIELFVDTPWYVEQPVRVPIDMSLSDAEIWAWAERDAHSR
                     AGFMPVEEWRAGLRAKLARSR"
BASE COUNT          388 a          734 c          709 g          367 t
ORIGIN      
        1 gtaacgcagc aatctgcgcc gagctggcgc gccgcatggc gcgcgtcctc ggcgatctcc
       61 aacggcacat gcatcgccgc cttgtcgtag gtcccggcgc atcgggcacc gagggcgttc
      121 gccacttgtc gcgccgaatc cgcttgctcc ggagtagaga gcacgagcgc gcgcctcgca
      181 ccgaggcggt cgagctcttc gggcaaacgc gcgagcgcgc cggcaccgaa aacgacccgc
      241 gccggaaggc cgttgtagac gaaggatctc actcgccttc tattccgcca cgatcttgtt
      301 gtcgcggatc agtcttccga gcttgtcgga ctcgccctgc acgaacttca cgaactcttc
      361 cggcgagctc gcgacgatct caaaaccctg ctcggtaagc ttgccgttca catccggcgc
      421 ctgcagcgtg cgcaccgttt cgcggtgaac tttcgctgcg acatccttag gcagacccgc
      481 cgggccgagg atgccgaacc aggaatattg ctcgaagccg gggaaggtct ccgacagagt
      541 ctgcacgtct gggagctgcg gcacgcgcgc gcggctgccg accgcgaggc ccttcatctt
      601 gcccgccttg atgtgcggca ccatcagcgg cagcgagaag aacgagatgt gcacctgctg
      661 gctgagcagc gcctggatca tcggcccgcc gcccttgtag gcgacgtgcg tcatcttgat
      721 gccggcaacc tgctcgagga gcagcgccgc caggtggttg gagctgcccg agcccggagt
      781 cgagtaggtc actttctccg ggttcgcctt ggcataggcc acgacctcgc gcaggttgtt
      841 gggcgcgaag ctcgtgacgc cgacgagcgc gctcggcgac ctcaccatca ggcagatgtg
      901 ctcgagctgc ttgaacgggt cgggcgccat cttgaagatg tggtggttca ccgcatgcgt
      961 gtcgaacacc atgagcaagg tgtagccgtc ggcgggcgcc ctggccacct cggccgcgcc
     1021 gaccgagccg ccggcgccgg agcgattctc gatcagcacc gtctggccca tgaactcctg
     1081 gaacctcggc tgcacgatgc gcgcgatcag gtcggtcgtg ccgccgggcg ggaacggaac
     1141 gatgatgcgc accggcttcg ccgggtagga ttgagcagac gcaaagcatg gaaccgcaac
     1201 cagcagcgcg gcgagcagct ttcgcatggc ctcctcctct cgccttcagt gtaacctagc
     1261 cccatgaaga agcagcagat ccgcacaagc aagctcgccg agccgagcgg gcacttttcg
     1321 caggcgatcg cggcgccggc caagggtgtg ctggtgttcg tgtccgggat gaccgcgcgc
     1381 cgcccggacg gcaaggtcgt tggcgtcggc gacgtggagg agcagacacg ccaggtgtgc
     1441 gagaacctca aggctgcgct cgaggccgcg ggcgggacgc tcgacgacgt ctgccgggtc
     1501 gacgtctatc tgcgcaacat cggcgaccgc gagcgcgtca acaaggtgcg ccggcagttc
     1561 tggcgcggca caccgcccgc ctcgacgctg gtcgaggtgt cgaagctcgc ctcacccgag
     1621 ttcttggtcg agatcaacgc gatcgcgcta gtttcgccct gaggcccgcg cgccactcct
     1681 cgaccggcat gaaccccgcc cggctgtggg catctcgctc ggcccacgcc cagatctcgg
     1741 cgtcggaaag actcatgtcg atcgggacgc gcaccggctg ctccacgtac cagggcgtgt
     1801 cgacgaaaag ctcgatgcgg ttgccttccg ggtccttgaa gtagaccgaa agcgcgttgc
     1861 cgtgcgacgc cggcgaaagt tctctcacgc cctcctcttc cagcctacgg tgcatttcgc
     1921 gcagcccggc gaaatcggcc atgcgaaaag agatctggtt gatggggttg aagccggccg
     1981 cggccggcct gcccgccgcc aggactatct gatggtgctc tctcgcatcg cggctcaaga
     2041 aaaccagcga aactgaacca agcatgcccc tgtcggtaac cgcaaaaccg aggacgcgtg
     2101 tgtaaaaatc ctccatccgc gcggggtcgg tcacgaagat ccccatgtgg ctgaacgaaa
     2161 actccggaac cgaggtcatc gcttatgagg ctcgctag
//
LOCUS       DASUNT010000218         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_993023, whole genome shotgun sequence.
ACCESSION   DASUNT010000218 DASUNT010000000
VERSION     DASUNT010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993023"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..711
                     /locus_tag="VFX94_04345"
     CDS             193..711
                     /locus_tag="VFX94_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEX5767446.1"
                     /translation="MEHWREQGGETASKPQGAATRWVTRERPKIDRIACPWLIRRFVD
                     PDAEFLYVPTPEVRRTAEEKTAVPYDIPDVEFSHEGELCSFDAFVRKFHLDKDPALAD
                     LALIVRGADTNRLDLAPQAAGLAAISLGLSRNFANDHEMLEHGMVVYDALYAWCKEGK
                     NEIHTWNPAQYR"
     gene            687..1976
                     /gene="chrA"
                     /locus_tag="VFX94_04350"
     CDS             687..1976
                     /gene="chrA"
                     /locus_tag="VFX94_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX5767447.1"
                     /translation="MEPRAIQVSFSEALRTWLKIGCLGFGGPAGQIAMMHRILVDEKK
                     WIDEPRYLHALNFCMLLPGPEAQKLATYSGWLLHGTRGGLVAGILFVLPGALVMLAFS
                     LLYVLGRGIPVVDGALFGIKAAVLVIVVEALIRIGRRSLKSRLLMAMAAAAFVGIFFF
                     DLPFPLIVALAGAIGFFTTRTTSAERASPMAGRWRQAASATVVGLVLWWAPVALALLA
                     LGKDHVFVDIGIFFSKLAVVSFGGAYALLAYMAQQAVETYGWMTAPEMVDGLGLAETL
                     PGPLIKVTQFVGFLGAYRAHEPLDAMTSAILGSALTTWVTFVPPMLLIFAGAPFVEQL
                     RSNQRLSGALAAITAAVVGVILNLTIWFVLHVLFADVQEARAGPLRWYAFDPLTIDLK
                     VSALALVAAVLAFVLHRGLVQLVLIMAALGITVRLLL"
     gene            1973..2260
                     /locus_tag="VFX94_04355"
     CDS             1973..2260
                     /locus_tag="VFX94_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX5767448.1"
                     /translation="MSAYIIAEIDITDAAAYEDYRKRVPDVIARYGGKYIVRGGKVES
                     LEGGWSPKRIAVLEFPSMEQALKFYRSAEYAPLIKVRQKASRGKLVSVEGV"
     gene            complement(2313..2834)
                     /locus_tag="VFX94_04360"
     CDS             complement(2313..2834)
                     /locus_tag="VFX94_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX5767449.1"
                     /translation="MRSGLALLFGAAALVGCQILGPSEEPRATAQLKPLGSHKAFGEV
                     TFDQMGKKVRLVANVQGLAPDREHGFHIHEVGDCNSADGMSAKGHFNPFGKPHGHFQS
                     GERHAGDLPALKADQNGRAKLEVELDTVTVTPGPASIVGRGLIVHADPDDYKTQPTGN
                     AGARLACAVIQRS"
BASE COUNT          449 a          931 c          944 g          520 t
ORIGIN      
        1 gtgcggcgca acgcccgctt ccttgaagct ggagagctga ttcgcggcgc cctgcgacgc
       61 gatccggaga agatcgagga gtggcgcaag accctgccga aggccgcccg cgtggtcgtg
      121 tactgcgtgc acggacacga agtgagccag aacgtcgcca gggcgctcgg ggcccggtac
      181 ctcgaaggcg gaatcgagca ttggcgtgaa cagggcggcg aaaccgcctc caaaccgcag
      241 ggcgcggcaa cccggtgggt gacccgcgag cggccgaaga tcgatcgcat cgcctgcccc
      301 tggctcatcc gtcgcttcgt cgaccccgac gccgagtttc tctacgtgcc gacgcctgaa
      361 gtaagacgaa ccgcggaaga aaaaaccgcg gtgccgtacg acatcccgga tgtcgagttc
      421 tcgcacgagg gggagctctg cagcttcgac gccttcgtca ggaagtttca tctggacaag
      481 gacccggcgc tggccgatct cgcgctcatc gtgcgcggcg ccgataccaa ccgcctcgat
      541 ctcgcgccgc aagcggcggg gctcgccgcg atctcgctcg gtctgtcgcg caacttcgcg
      601 aacgaccacg agatgctcga gcacggcatg gtggtctacg acgcgctcta cgcatggtgc
      661 aaagaaggca agaacgaaat ccatacgtgg aaccccgcgc aatacaggta agtttcagcg
      721 aagcgctccg tacctggctc aagatcgggt gcctgggctt tggcgggccc gccggccaga
      781 tcgcgatgat gcatcgcatc ctggtcgacg aaaagaaatg gatcgacgag ccgcgctatc
      841 tgcatgcgct caacttctgc atgctgctgc ccgggcctga ggcgcagaag ctcgctacct
      901 actcgggctg gctgctgcac ggcacgcgcg gcgggctcgt ggccgggatc ctgttcgtgc
      961 tgcccggcgc gctcgtcatg ctggccttca gcctgctcta cgtgctggga cgcggcatcc
     1021 cggtggtcga cggggcgctc ttcggcatca aggcggcggt gctggtgatc gtggtggagg
     1081 cgctgatccg catcggccgc cgctcgctca agtctcggct cctcatggca atggcggcgg
     1141 ccgcgttcgt cggcatcttt ttcttcgacc tgccgttccc gctcatcgtg gcgctcgccg
     1201 gtgcgatcgg cttcttcacg acaagaacga catcggcgga acgtgcttca cccatggctg
     1261 ggcgctggcg gcaagccgca agcgcaaccg tggtcgggct cgtgctctgg tgggcgcccg
     1321 tggcgctcgc gctccttgcg ctgggcaagg accatgtgtt cgtagacatc ggcatcttct
     1381 tctcgaagct tgccgtggtg agcttcgggg gcgcgtacgc gctgctcgcc tacatggcac
     1441 agcaggcggt ggagacctac ggctggatga cggcacccga gatggtcgac ggactggggc
     1501 ttgccgagac gcttcccggt ccgctcatca aggtcacgca gttcgtcggc ttcctgggcg
     1561 cctacagagc gcacgagccg cttgatgcaa tgaccagtgc catcctggga tcggcgctga
     1621 cgacctgggt cacgttcgtg ccgccgatgc tcctcatttt cgcgggggcg ccgttcgtcg
     1681 agcagctcag atccaatcag cgcctctccg gcgcgctcgc cgcgatcacc gcggcggtgg
     1741 tcggcgtgat cctcaacctg acgatctggt tcgtgctgca cgtgcttttc gccgacgtgc
     1801 aggaggcgcg cgccggaccg ctgcgctggt acgccttcga ccccctgacg atcgacctca
     1861 aggtctcggc gctcgcgctg gtggccgcgg tgctcgcctt cgtgctgcac cgcgggctgg
     1921 ttcagcttgt gctcatcatg gccgcgctcg ggatcacggt aagattgctc ctatgagcgc
     1981 ctacatcatt gccgaaatcg acattaccga cgccgcggcg tacgaggact accgcaagcg
     2041 ggttcctgac gtcattgcgc gctatggcgg gaaatacatc gtccgcggcg gcaaggtgga
     2101 gtcgctggag ggcggatggt cgcccaagcg catcgccgta ctggagttcc cctcgatgga
     2161 gcaggccctc aagttctatc gctcggcgga gtacgcgccg ctcatcaagg ttcgacagaa
     2221 ggcctcgcgc ggcaagctgg tgagcgtcga aggcgtatag aaacgaaagg gcgtctcgcg
     2281 acgccctttc ggcccctcct cctccagtat ctctagcttc gctggatcac ggcgcacgca
     2341 aggcgagcgc cggcattgcc cgtgggctgg gtcttgtagt cgtccggatc ggcgtgaacg
     2401 atgagcccgc ggccgacgat gctcgccggc cccggcgtga ccgtgacggt gtcgagctct
     2461 acctcgagct tggcgcggcc gttctggtcc gccttgagcg cgggcaggtc gcccgcgtgg
     2521 cgctcgccgg attggaaatg gccgtggggc ttgccgaacg gattgaagtg ccccttggcg
     2581 ctcatgccat cggccgaatt gcagtcgccg acctcgtgga tgtggaaccc gtgctcgcgg
     2641 tcgggcgcta ggccctgcac attggccacg aggcgcacct ttttgcccat ctgatcgaag
     2701 gtcacctcgc cgaacgcctt atggctgccc agcggcttga gctgcgccgt agcgcgcggc
     2761 tcttcgctcg ggccaagaat ctggcagccg acaagcgccg cggccccgaa taagagcgcg
     2821 agccccgacc tcatttattg gcgc
//
LOCUS       DASUNT010000219         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1081252, whole genome shotgun sequence.
ACCESSION   DASUNT010000219 DASUNT010000000
VERSION     DASUNT010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081252"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VFX94_04365"
     CDS             complement(<1..1002)
                     /locus_tag="VFX94_04365"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX5767450.1"
                     /translation="MNAAPSVNARTERLLAAPIVPTLARLSAPGVLLVAFQSLVSVGD
                     MYFVGRLGTGPLAGLALVFPLLMLLQMTSAGAMGGGVSSAIARALGSGDAVAARRLVV
                     HALVIATAMGAAFTILVLLLGESIYELLGGRAGTLEHALRYSSIVFGGAIVVWIANTL
                     SSVLRGSGNMLTPALALIGAALVHLPLSGILVSRIGIAGAGIAYVTTFGIAALVMGLI
                     VRRSSLLSPRREDWKLEWRLFREILRVGAISTLSAVQTVLTAVILTGFVGRHGAAALA
                     GYGVGLRLELLQIPLVFAVGQALVVLVGTHIGAGRAARAKRIAWVGASFAACISLVIG
                     "
     gene            1052..1795
                     /locus_tag="VFX94_04370"
     CDS             1052..1795
                     /locus_tag="VFX94_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX5767451.1"
                     /translation="MIELLQDPQVWIAFFTLTALELVLGIDNIIFISILVDKLPPAQR
                     DRARRIGLGAAMLMRIGLLLVLAWMIGLTEPLFTVFNERLSGRDLILIGGGLFLLWKS
                     VMEIHQLLEGEEGERSAAVRASFSAVIGQIMVIDLIFSLDSIITAVGMVPWIEVMIAA
                     VIASVALMMAFAGPIGRFVSAHPTIKMLALSFLVLIGVLLIAAGFDHDVPKGYVYFAL
                     AFSVAVEMLNIRLRKRSKPVRLHEPYSRG"
     gene            complement(1780..>2143)
                     /locus_tag="VFX94_04375"
     CDS             complement(1780..>2143)
                     /locus_tag="VFX94_04375"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX5767452.1"
                     /translation="MRKMLVAAGWGWAAAIVWLSLTPSPPELDIDHGDKLGHIAAYGL
                     LMFWFAQLYVARPSRVAHAVVFLAMGVGLEFLQGALGYRSYDIFDMYANTLGVALGWA
                     LGAMLRPRFPRPADQPRL"
BASE COUNT          369 a          730 c          725 g          319 t
ORIGIN      
        1 gccgatcacg aggctgatgc aggcggcgaa gctcgcaccg acccaggcga tgcgcttcgc
       61 gcgcgcagcg cgcccggcgc cgatgtgtgt gccgacgagc accacgagcg cctggccgac
      121 cgcgaatacc agcgggatct gcaggagctc tagtcgaagt ccgaccccgt agccggcaag
      181 cgccgccgcg ccatggcgcc cgacgaagcc ggtgaggata acggccgtca gcacggtttg
      241 aaccgccgaa agcgtcgaga tcgcgcccac gcgcaggatc tcgcggaaca gccgccattc
      301 gagcttccag tcctcgcgcc ggggcgagag caggctcgag cgccgcacga tcagccccat
      361 gacgagcgcc gcgatcccga aagtcgtgac gtaggcgatt cccgcgcccg cgatgccgat
      421 gcgtgagacg agaattccgg agagcggcag gtgcacgagc gccgcgccga tcagcgcgag
      481 cgcgggggtg agcatgttgc cgctcccgcg cagcacgctc gagagcgtgt tggcgatcca
      541 gaccacgatc gcgccgccga agacgatgct cgagtagcgc agcgcgtgct cgagggtccc
      601 ggcccggccg ccgagcagct cgtagatcga ttctccaagc aacaagacca ggatcgtgaa
      661 ggcggcgccc atcgctgtgg cgatgacgag cgcatgcacg accagtcgcc gggcggcgac
      721 cgcgtccccg gagcccagag cgcgcgcgat cgccgaagac accccgccgc ccatcgcgcc
      781 agcggaagtc atctgcagca gcatgaggag cgggaacacg agcgcgaggc cggcgagcgg
      841 cccggtgccg aggcggccga cgaagtacat atcgccgacc gaaaccagcg actggaatgc
      901 gaccagcaac acgcccggcg ccgacaggcg cgcgagcgtc ggtacaatcg gcgcggcgag
      961 cagccgttcg gtgcgcgcat tcacggaggg cgccgcattc atggggcgcg ccattgtatg
     1021 atcgcgcgct tgcacgcagg aggatgacgc cgtgatcgag ctccttcagg accctcaagt
     1081 ctggatcgcc ttcttcacgc tcaccgcgct cgagctcgtg ctcggcatcg acaacatcat
     1141 cttcatctcg atcctggtcg acaagctgcc gccggcgcag cgtgaccgcg cgcggcgcat
     1201 cggcctcggc gccgcgatgc tgatgcgcat cgggctgctc ctggtgctcg cctggatgat
     1261 cgggcttacc gagcccctct tcactgtgtt caacgaaagg ctgtccgggc gcgacctgat
     1321 cctgatcggg ggagggctgt tcctgctctg gaagagcgtg atggagattc accagctgct
     1381 cgaaggcgag gagggcgagc gctcggctgc cgtgcgcgcc agcttctctg ccgtgatcgg
     1441 ccagatcatg gtgatcgacc tcatcttttc gctcgactcg atcatcaccg cggtcgggat
     1501 ggtgccgtgg atcgaggtga tgatcgctgc ggtgatcgcg tcggtggcgc taatgatggc
     1561 attcgccggg ccgattggtc gcttcgtctc ggcgcacccg acgatcaaga tgctcgcgct
     1621 ctctttcctg gtcctgatcg gcgtgctgct gatcgcggcc ggcttcgacc acgacgtccc
     1681 caagggctac gtatactttg cgctcgcttt ctccgtcgcg gtggagatgc tcaacatccg
     1741 cctgcgcaag cgctccaagc ccgtgcgcct gcacgagccc tacagccgcg gctgatcggc
     1801 cgggcgcggg aagcgcgggc gaagcatcgc gccgagcgcc cagccgagtg cgacgccgag
     1861 cgtgttcgca tacatgtcga agatgtcgta gctgcggtag ccgagggcgc cctgcaggaa
     1921 ttcgaggccc acgcccatcg ccaggaacac gacggcgtgc gcgacgcggc tcggccgcgc
     1981 cacataaagc tgcgcgaacc agaacatcag cagcccgtag gcggcgatgt gccccagctt
     2041 gtcgccgtgg tcgatgtcga gctcgggtgg cgagggcgtg agcgacagcc acacgatcgc
     2101 cgcggcccac ccccagccgg cggcgaccag catcttcctc att
//
LOCUS       DASUNT010000220         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_993120, whole genome shotgun sequence.
ACCESSION   DASUNT010000220 DASUNT010000000
VERSION     DASUNT010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993120"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="VFX94_04380"
     CDS             complement(<1..1340)
                     /locus_tag="VFX94_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX5767453.1"
                     /translation="MHTRLRNIGFMIVGAVAGVLISLNFQAIADRATRSSLPIEELRA
                     FTEVFGAIKTNYVEPVEDKKLITEAINGMLTGLDPHSSYLDAEAFRELQVGTQGQFGG
                     LGIEVGMEDGFVKVISPIEDTPAFRAGIKPGDLIIKLDDTPVKGMTLNDAVKRMRGKP
                     NTQITLTISRKGESAPMVVTITRAVIRVQSVKSKVMEPGYAWVRVSQFQEATAEHLVK
                     HLEGLFKQGQVKGLVLDLRNDPGGLLHGAVAISSAFLPPKSLVVSTDGRAEDARRKFY
                     ASPDDYARRGDDVLKALPQAAKTVPMVVLVNGGSASASEIVAGALQDHKRAKVIGTQT
                     FGKGSVQTIMPLGNNTAIKLTTARYYTPSGRSIQALGITPDIMVEDPSDTLVRVREAD
                     LQRHLENGRAEAEKKDSTVTRTKPPATPAETKPTELGSKEDFQLQQAIAFLKGEP"
     gene            complement(1350..2150)
                     /locus_tag="VFX94_04385"
     CDS             complement(1350..2150)
                     /locus_tag="VFX94_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX5767454.1"
                     /translation="MLAAWASAGTPEALRVALSGNDPADIGRALHYLGHISRAAAQLL
                     AEQRAALAELERLATEAQARGERLRAIEQASRADRDKLLSERRNRSRVLASLSTEIQK
                     NRKEIRVLRADEARLARLVEALGRSGFPSAKGAFSGLRGKLVLPVRGELTQRFGAPRG
                     AAGTEAKGVFIRAPQGQPVRAIAKGQVVYADWMRGFGNLLIVDHGESYLSIYANNESL
                     LKEPGETVSPGEPIATTGSSGGNEQTGLYFEMRHLGRAFDPLSWVKKK"
BASE COUNT          323 a          789 c          765 g          426 t
ORIGIN      
        1 ggctcgccct tgaggaaagc gatcgcctgc tggagctgga agtcttcctt ggagccgagc
       61 tcggtcggct tggtctccgc gggcgtcgcg ggcggcttgg ttcgcgtgac cgtggagtcc
      121 tttttctccg cctcggcgcg gccgttctcg aggtggcgtt ggaggtccgc ttcgcgcacg
      181 cgcaccagcg tgtcgctcgg atcctcgacc atgatgtcgg gcgtgatgcc gagcgcctgg
      241 atggagcgcc cgctcggcgt gtagtagcgg gcggtagtga gcttgatcgc ggtgttgttg
      301 ccgagcggca tgatcgtctg caccgagccc ttgccgaagg tctgcgtgcc gatcaccttg
      361 gcgcgcttgt ggtcctgcag cgcgcccgcc acgatctcgg acgccgacgc cgagccgcca
      421 ttcacgagca cgaccatcgg cacggtcttc gccgcctgcg gcagcgcctt cagcacgtcg
      481 tcgccgcggc gcgcatagtc gtccgggctt gcgtagaact tgcgtcgcgc gtcctcggcg
      541 cggccgtcgg tcgagacgac gagcgacttc ggcggcaaga acgccgacga aatggcgacc
      601 gcgccgtgca gcaggccgcc ggggtcgttg cgcaggtcga gcaccaggcc ctttacctgt
      661 ccttgcttga aaagcccttc caggtgcttc accaggtgct cggcggtcgc ttcctggaac
      721 tgcgacacgc gcacccaggc gtagcctggc tccatgacct tggacttcac gctctgcacg
      781 cggatgaccg cgcgcgtgat cgtgaccacc atcggcgcgc tctcgccctt gcgcgagatg
      841 gtgagcgtga tctgggtgtt gggcttgccg cgcatgcgct tcaccgcgtc gttcagcgtc
      901 atgcccttca ccggcgtgtc gtcgagcttg atgatcaggt cgcccggctt gatgccggcg
      961 cggaacgccg gcgtgtcctc gatcggcgag atcaccttga cgaagccgtc ttccatgccg
     1021 acctcgatgc ccaggccgcc gaactggccc tgcgtgccga cctgcagctc gcggaacgcc
     1081 tccgcatcga gatacgagga atgcgggtcg aggccggtca gcatcccgtt gatcgcctcg
     1141 gtaatgagct tcttgtcctc caccggctcc acgtagttcg tcttgatggc gccgaagacc
     1201 tcggtgaagg cgcgcagctc ctcgatcgga agcgacgagc gcgtcgcccg gtcggcgatc
     1261 gcctggaagt tgaggctgat cagcacgccg gcaacggcgc cgacgatcat gaagccgatg
     1321 ttgcgaagcc tggtatgcat gacgctcgct tatttcttct tgacccacga cagcgggtca
     1381 aaggcgcggc ccaagtgccg catttcaaag tataaacccg tttgctcgtt accaccgctc
     1441 gatccggtgg tggcgatcgg ctctcccggc gacacggttt cgcccggctc cttcagcagc
     1501 gactcattgt ttgcgtagat tgacaggtaa ctttcgccgt ggtccacgat caggaggttg
     1561 ccgaatccgc gcatccagtc ggcgtagacc acctggccct tggcgatggc gcgcaccggc
     1621 tgcccctgcg gagcgcgaat gaatacgcct ttcgcctccg tccccgccgc tccccttgga
     1681 gccccgaacc gctgcgtcag ttcccctcgc accggaagaa ccagcttgcc gcgcaggccg
     1741 gaaaaagccc ctttcgcgga gggaaatccg ctgcgtccga gggcctcgac gaggcgcgcg
     1801 aggcgcgctt cgtcggcccg cagcacgcgg atttccttac gatttttttg gatctcggtg
     1861 gagaggctgg cgagcacgcg agagcggttg cggcgctcgc tgagcagttt gtcacgatcg
     1921 gcgcggcttg cctgctcgat cgcgcgcaat cgctcgccgc gcgcctgcgc ctcggttgcg
     1981 agccgctcga gctcggccag cgcggcgcgc tgctcggcga gcaattgcgc cgccgcacgc
     2041 gagatgtgtc cgaggtaatg cagcgcgcga ccgatgtcgg caggatcgtt tcccgacagc
     2101 gccacgcgca gcgcctcggg cgtgccggcg ctcgcccacg cggcgagcat gcgctcgatc
     2161 gcagcctcgc gcttcgccac ctgcgcctgc agcgactcgc gctgctgccc gacccgcgca
     2221 gcctcggcgc gaaccgtgcg cgcttcggcg ccgagccggg cgagcgcgcg gttcgcgttc
     2281 gagatggcgc gctcggaatc gcg
//
LOCUS       DASUNT010000221         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_905712, whole genome shotgun sequence.
ACCESSION   DASUNT010000221 DASUNT010000000
VERSION     DASUNT010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905712"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1005)
                     /locus_tag="VFX94_04390"
     CDS             complement(55..1005)
                     /locus_tag="VFX94_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HEX5767455.1"
                     /translation="MRVLVTGTAGFIGMHCAKRLLERGDEVIGIDNLSPYYSVQLKKD
                     RLALLEHPKFAFTQLDIADGATLRKYFESNQPDQVLHLAAQAGVRYSLENPGAYIEAN
                     LVGFANLLECCRRHPPGHLVYASSSSVYGANPQLPWSESQNVDQPVSLYAATKKSNEL
                     LAHAYSHLFGLNTTGLRYFTVYGPWGRPDMSAFLFAQAILQGKPIPVFNHGDMKRDFT
                     YIDDIVEGTLRVLDRPARNAVYNIGNHQPVALLDFIAALERAFGKKAKLDLRPMQPGD
                     VKSTYADTSALRAAVGFAPSTPLETGLRRFAEWYREYYKT"
     gene            complement(1002..1769)
                     /locus_tag="VFX94_04395"
     CDS             complement(1002..1769)
                     /locus_tag="VFX94_04395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5767456.1"
                     /translation="MPRAPLSAVLITRNAAAVLEPCLDSLAFADEIVVVDSASSDGTV
                     ELATRKGARVVQKEWLGFGRQKQFAVERASHDWVLCVDADERVSPQLAQSIQDALAAP
                     ASPVYRMARRNRFLGRWLSHGEGYPDWSPRLFNRMNARWSDDLVHEKVLFAVTPGTLR
                     GDLMHESAEDLSAYLERQNRYTTLAARQAFEEGRSVSLAHLFLSPVVRFIKFYLMRLG
                     FLDGLPGLLHISIGCINSYVKYMKLIELHQAEKQAAR"
     gene            1811..>2124
                     /locus_tag="VFX94_04400"
     CDS             1811..>2124
                     /locus_tag="VFX94_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEX5767457.1"
                     /translation="MRTILFVKTSSLGDVVHNCPAVSDAARALPGAAIDWVVEEPFAG
                     IAAMHRAVRRVIPVAMRRWRSALWKPSVWTEIDAWRTELRRERYDAVVDTQSLFKSAL
                     ITR"
BASE COUNT          362 a          669 c          723 g          370 t
ORIGIN      
        1 attcacggtt gaagaggggt tgcgggcaac ggccgactgg taccggcaaa cggcttaggt
       61 cttgtagtac tccctgtacc actcggcgaa gcgccgcagc ccggtttcaa gcggcgtgga
      121 cggcgcaaaa cccacggcgg cgcgcaacgc gctcgtatct gcgtaggtcg acttgacgtc
      181 gccgggctgc atgggccgca agtcgagctt cgccttcttt ccgaaggcgc gctcgagcgc
      241 cgcgatgaag tcgagcagcg cgaccggctg gtggttgccg atgttgtaga ccgcgttacg
      301 cgccggccgg tcgagcacgc gcagcgtgcc ctccacgatg tcgtcgatgt aggtgaagtc
      361 gcgcttcatg tcgccgtggt tgaacaccgg gatcggcttg ccctgcagaa tcgcctgggc
      421 gaacagaaac gccgacatgt ccggccggcc ccacggcccg tacacggtga agtaacgcag
      481 gcctgtggtg ttgagtccga aaaggtggct gtaggcgtgc gcaaggagct cgttggactt
      541 cttcgtcgcc gcgtacaggc tcacgggctg gtccacgttc tgcgactccg accagggaag
      601 ctgcgggttc gcgccgtaca cgctcgagct cgacgcgtac acgagatggc cgggcgggtg
      661 gcgtcggcag cattcgagca ggttcgcgaa cccgaccaga ttggcctcga tatatgcgcc
      721 cgggttctcc agcgagtagc gcacgcccgc ttgcgcggcg aggtgcagca cctgatcggg
      781 ctggttgctt tcgaaatact tccgcaaggt cgcgccgtcg gcgatatcga gttgcgtgaa
      841 cgcaaacttc gggtgctcga gcagcgcgag gcgatccttc ttgagctgca ccgagtagta
      901 aggcgaaagg ttgtcgatgc cgatcacctc gtcaccgcgc tcaagcagcc gcttcgcgca
      961 gtgcatgccg atgaagcccg cggtcccggt gacgagaacc ctcaacgcgc cgcttgcttc
     1021 tccgcctggt gcagctcgat cagcttcatg tacttgacgt agctattgat gcagccgatc
     1081 gagatgtgga gcagtccggg caggccgtcg aggaacccaa ggcgcatcag gtagaacttg
     1141 atgaagcgca ccacgggcga gaggaatagg tgcgcaaggc tcacgctacg cccctcctcg
     1201 aacgcctggc gcgcggcgag ggtggtgtag cggttctggc gctcgagata cgccgagagg
     1261 tcttccgcgg actcgtgcat caggtcgccg cgcagcgtgc ccggcgtgac ggcgaaaagc
     1321 actttctcgt gcaccaggtc gtcgctccag cgcgcattca tgcggttgaa aaggcgcggg
     1381 ctccagtcgg ggtagccctc gccgtgcgag agccagcggc cgagaaagcg gttgcgccgc
     1441 gccatgcggt acacgggaga agcgggcgcg gcgagcgcat cctggatcga ctgcgcgagc
     1501 tggggcgaca ctcgctcgtc ggcgtcgacg cagagcaccc agtcgtggct cgcgcgctcg
     1561 accgcgaact gcttctggcg gccgaagccg agccactcct tctgtaccac gcgcgcgccc
     1621 ttgcgcgtcg cgagctccac cgtgccatcg ctcgacgccg agtccaccac cacgatttcg
     1681 tcggcgaacg cgaggctgtc gaggcatggc tcgagtacag cggcggcgtt gcgcgtgatg
     1741 aggaccgccg agaggggcgc tctgggcaag ttcgctattc ccttaaaatc ggcgtgccgt
     1801 attctagcca atgagaacaa tactcttcgt caagacttcc tcccttggcg atgtggtgca
     1861 caactgtccc gcggtgagcg atgccgcgcg cgcgttgccc ggcgcggcga tcgactgggt
     1921 cgtggaggag ccgtttgccg gaatcgcggc gatgcaccga gcggtgcgcc gcgtgatccc
     1981 ggtcgcgatg cgtcgctggc gctccgcgct gtggaagccc tcggtatgga ccgagatcga
     2041 cgcgtggcgc accgagctgc gccgagaacg atacgacgcg gtcgtcgaca cgcagtcgct
     2101 cttcaagagc gcgctcatca cccg
//
LOCUS       DASUNT010000222         4128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_555262, whole genome shotgun sequence.
ACCESSION   DASUNT010000222 DASUNT010000000
VERSION     DASUNT010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555262"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VFX94_04405"
     CDS             <1..338
                     /locus_tag="VFX94_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5767458.1"
                     /translation="AARRFCAPGMEIRFTGNRLMHDPAEATAFNKARYKWVKKKFGPT
                     HVVAGGTPEETIVYQTGHLYGEWPDGTPFEGNRYVDRYVVRHGKIWKMEVWNDSAEWL
                     LVRAGLAKP"
     gene            343..1215
                     /locus_tag="VFX94_04410"
     CDS             343..1215
                     /locus_tag="VFX94_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5767459.1"
                     /translation="MQRVRPLKSHGRYPYSAITRRRDYNWPGGKRLALYIGFNVEHFD
                     FGAGLGAALGPKSPEPDVLNYSWRDYGNRVGVWRCFELFDSLKLKVGALINTALYDYC
                     PDVVEAHVKRGDELIGHGHTNSDRQSDLSEERERELLLYCRETIRKRSKVTPLGWLSP
                     WISESRVTPDLLQETGYKYTLNWCHDDQPIEMQTRAGKLWSIPYPQELNDIPMIALRQ
                     MDGRDFADMIVDNLDEMLRQSKEQALVMGIALHPYLVGQPYRLKHLRRALQHVVKAKK
                     VWLTTPARILEAVS"
     gene            complement(1212..2084)
                     /locus_tag="VFX94_04415"
     CDS             complement(1212..2084)
                     /locus_tag="VFX94_04415"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX5767460.1"
                     /translation="MSFPASATEQGLQARAGELLIAGSLEISPRELHRAGEVAALLPA
                     NTCVYIPSLPGLPLSRTLEAIAAMRAAGLDPVPHVSARRILNEDEFRAFLKEAVAKHG
                     VHRVLLIGGDESKPKGPFVDSLQILESGLLADSGIREIGVAGYPEGHPKIPGSSLLAA
                     FEEKLRLTREQGLGLYVVTQFSFAPHRVVEYCANLARTAPEVSIYAGIAGPTNPVALA
                     RYAQRCGVSVSLRALRALGSGMARLVANSDPREQLITLARYSVQREPSNLVGVHIYSF
                     GGAVRTAAWMRELI"
     gene            complement(2059..3465)
                     /locus_tag="VFX94_04420"
     CDS             complement(2059..3465)
                     /locus_tag="VFX94_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767461.1"
                     /translation="MEIAVAGSLLGSISEFCRRTGMAESTFGRRAVNDGKFVSRLRDG
                     ARVTPETLHRVNQFMVKHGAPGAGDSPPELLPLIRASAVPASAAAQASEAPPAKNFRF
                     FDNRQKYLLFVNTCSEKDVVASRVGLELGHIHPRPPAVRLFDAGMGDGTVLAQVMREM
                     HRRFPTVPFYIVGKEISLEDVRLSLRKMADRFYEHPATVLVVTNMYYTEAPWLTPRTV
                     PAATSMVWHEAALTGTTSHDFAEQIAALEPFLARNWQARHSPKTGNPVYEHPVALVIY
                     RDDYRFMLDEVVPRRGHGKADYDMVIASQPYRLRAPLEFKAQKVIAPLVRALGKGGRL
                     LGIHSHGEDPGLEIIQKVWPGENPYMQSRHDILRATKTELGREARRYNFNAYADTRAI
                     FRYEMHTLPSEISDSIGTSTLFAAWNAAIYVAQIDDVRLSEVLRDTRYLDATREVLQK
                     HGSLWFLDESYVVSRKRD"
     gene            3544..>4128
                     /locus_tag="VFX94_04425"
     CDS             3544..>4128
                     /locus_tag="VFX94_04425"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEX5767462.1"
                     /translation="MNQRVNVLDLIGNTPMIEVQRLDAGRCRLFLKLENHNPGGSIKD
                     RVALAMIEAAERDGRLSPGGLLVEATAGNTGLGLALVAAQKGYRLLIVVPDKMSQEKI
                     FHLKAMGAEVVMTRSDVTKGHPEYYQDMAARLAQEKSAFYINQFANPANPKAHEESTG
                     PEIWRQMEQHVDAVVAGVGTGGTISGLARFFERVS"
BASE COUNT          741 a         1323 c         1387 g          677 t
ORIGIN      
        1 acgcggcccg gcggttctgc gcccccggga tggaaattcg ctttaccggc aaccgcttga
       61 tgcacgaccc ggccgaggcc accgcgttta acaaggcgcg ctataaatgg gtgaagaaga
      121 aattcgggcc gacgcacgtc gtcgcgggcg gcaccccgga ggagaccatc gtctaccaga
      181 ccggccacct gtacggcgaa tggcccgacg gcacgccgtt cgagggcaac cgctacgtcg
      241 accgctacgt ggtgcgccac ggaaagatct ggaagatgga agtctggaac gacagcgcag
      301 agtggctgtt ggtgcgcgcg ggcctcgcga agccctgatt tggtgcaacg ggtgcgtccc
      361 ctcaagagcc acggacgcta cccttattcc gccatcacgc ggcgccggga ctacaactgg
      421 cccggcggca agcgcctcgc cctctacatc ggcttcaacg tcgagcattt cgatttcggc
      481 gccggcctcg gcgcggcgct cggtccgaaa agccccgagc ccgacgttct caattactcg
      541 tggcgcgact acggcaatcg ggtcggcgtc tggcgctgct tcgagctttt cgactcgctc
      601 aagctcaagg tgggcgcgct catcaacacc gcgctctacg actactgccc ggacgttgtc
      661 gaggcgcacg tcaagcgcgg cgacgagttg atcggccacg gccataccaa ttcggatcgg
      721 cagagcgatc tgtccgaaga gcgcgaacgc gagctcctcc tctactgcag ggaaacgatc
      781 cgcaagcgat ccaaggtgac cccgctcggc tggctgagcc cctggatttc cgagagtcgt
      841 gtcacgcccg atctgctgca ggaaacaggc tacaaataca cgctcaactg gtgccacgac
      901 gaccagccga tcgagatgca gacgcgcgcg ggtaagctct ggtctatccc ttacccgcag
      961 gagctgaacg acatcccgat gatcgcgttg cgtcagatgg acggccgcga cttcgccgac
     1021 atgatcgtcg acaacctgga cgagatgctg cggcaatcaa aggagcaggc gctcgtcatg
     1081 ggcatcgcgc tgcatccgta cctcgtcggc cagccttacc gcttgaaaca cctgcgccgt
     1141 gcgctgcagc acgtggtcaa agcaaagaag gtctggctca ctacgccggc gcgcatcctc
     1201 gaagccgtca gctagatcag ctcgcgcatc caggcggcgg tgcgcacggc gccgccgaag
     1261 ctgtagatgt ggacgccgac gaggttgctc ggctcgcgct gcacgctgta gcgcgcgagc
     1321 gtgatgagct gctcgcgcgg gtcggaattg gcgaccagac gcgccattcc gctgccgagc
     1381 gcacgaagcg cgcggaggga aacgctgacg ccgcagcgct gggcgtagcg ggcgagagcc
     1441 accgggttcg tcgggccggc gatgccggcg tagatcgaga cctcgggcgc tgtgcgcgcg
     1501 aggttcgcgc agtactcgac gacccggtgc ggcgcgaacg agaactgggt cacgacgtaa
     1561 aggccgagcc cctgctcgcg cgtcaggcgc aatttctctt cgaacgctgc gaggagagaa
     1621 gaacccggaa tctttggatg cccttcgggg tagccggcga cgccgatctc gcggatgccg
     1681 gaatcggcaa gcagccccga ttcgaggatc tgcaggctgt cgacgaacgg acccttgggc
     1741 ttggattcgt cgccaccgat gagcagcacg cggtgcacgc cgtgctttgc caccgcctcc
     1801 ttgaggaacg cgcgaaattc atcctcattg agaatccggc gcgccgatac gtgcggcacc
     1861 gggtcgaggc cggccgcgcg catggcggcg atcgcctcga gggtgcgcga gagcggcagc
     1921 ccgggaagcg acgggatgta cacgcacgtg ttcgcgggaa gcagtgccgc cacctcgccg
     1981 gcgcgatgca gctcgcgcgg ggagatttcg agcgaaccgg cgatcagcag ctcgccggcg
     2041 cgcgcctgca ggccctgctc agtcgcgctt gcgggaaacg acatacgact cgtcgagaaa
     2101 ccaaaggctg ccatgttttt gcaggacctc gcgtgtagcg tcaaggtagc gggtgtcgcg
     2161 cagtacttcg gaaaggcgca cgtcgtcgat ctgcgcgacg tagattgcgg cgttccaggc
     2221 cgcgaacagc gtcgaggtgc cgatgctgtc ggagatctcg ctcggcagcg tatgcatctc
     2281 gtagcggaag atggcgcgcg tgtcggcgta ggcgttgaag ttgtagcgcc gcgcctcgcg
     2341 gccgagctcg gtcttcgtcg cgcgcagtat gtcgtggcgg ctctgcatgt atgggttctc
     2401 gccaggccat accttctgga tgatctccaa gccggggtcc tcgccgtgcg agtggatgcc
     2461 gaggagcctt ccccccttgc cgagcgcgcg cacgagcggc gcgatgacct tctgcgcctt
     2521 gaattcgagc ggcgcgcgca ggcggtaggg ctgcgaggcg atcaccatgt cgtagtcggc
     2581 cttgccgtgg ccgcgccgcg ggacgacttc gtccagcatg aagcgatagt cgtcgcggta
     2641 gatgacaagc gccacgggat gctcgtagac cgggttgccc gtcttcgggc tgtggcgggc
     2701 ctgccagttc cttgcgagga agggctcgag cgccgcgatc tgctcggcga agtcgtgcga
     2761 cgtcgtgccg gtgagcgcgg cctcatgcca caccatcgag gtcgcggccg gcaccgtgcg
     2821 cggcgtgagc cacggcgcct cggtgtaata catgttcgta accacgagca cagtggcggg
     2881 gtgctcgtag aagcggtcgg ccatcttgcg caggctcagg cgcacgtcct cgaggctgat
     2941 ctccttgccc acgatgtaga acggcaccgt cgggaagcgc cggtgcatct cgcgcatgac
     3001 ctgggcgagg accgtgccgt cgcccatgcc cgcgtcgaag aggcgaaccg ccggggggcg
     3061 tggatggatg tggccgagct ccaggccgac gcgcgaggcg accacgtcct tctcgctgca
     3121 cgtgttgacg aaaagcaggt acttctgccg gttgtcgaag aaacggaagt tcttcgcggg
     3181 cggcgcttca cttgcctgcg ccgccgccga cgccggcacg gcgctcgcgc ggatcagcgg
     3241 cagcagctcc ggcggggaat cgcccgcgcc cggcgcgcca tgcttcacca tgaactggtt
     3301 gacccggtgc agggtctcag gggtgacgcg cgcgccgtcg cgcaaccgcg agacgaactt
     3361 gccgtcgttc accgccctgc ggccaaaagt cgattccgcc attcccgtgc gacggcagaa
     3421 ttcggaaatg ctgcctagca ggcttccggc tacggcaatc tccatcgctc ctcctgcggc
     3481 aatagtgggc aatgctgccg tgggacggca accacgacct ggcatgggaa gtatcccacc
     3541 attatgaatc agcgagttaa cgtcctcgac ttgattggaa atacgcccat gatcgaggta
     3601 cagcggctgg atgccgggcg ttgccgcctt ttcctcaagc tggaaaacca caatcccggc
     3661 ggctcgatca aggaccgagt cgccctcgcc atgatcgaag cggccgagcg cgacggacgg
     3721 ctttcccctg gcggcctgct ggtggaagcg accgcgggca acacggggct cggactcgcg
     3781 ctcgtcgcgg cgcagaaggg ctaccggctc ctcatcgtgg tgcctgacaa gatgagccag
     3841 gagaagatct tccatctcaa ggcgatgggc gccgaggtgg tgatgacccg ttcggatgtc
     3901 acgaaaggcc acccggagta ttaccaggac atggcggcgc ggctcgcgca ggaaaagagc
     3961 gctttttaca tcaaccagtt cgccaatccg gcgaacccga aagcacacga agaaagcacc
     4021 ggccccgaga tctggcgcca gatggagcag cacgtcgacg cggtggtcgc cggcgttggc
     4081 acgggcggca cgatcagcgg cctcgcgcgc ttcttcgagc gcgtatcg
//
LOCUS       DASUNT010000223         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_292886, whole genome shotgun sequence.
ACCESSION   DASUNT010000223 DASUNT010000000
VERSION     DASUNT010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292886"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="VFX94_04430"
     CDS             <1..1268
                     /locus_tag="VFX94_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX5767463.1"
                     /translation="VLERLNTLKVKAEFAGCPVEFWDMYGASGLPVRATVSDMGPLLL
                     ARMLDLNETQAGVLSLVFKIADDHGLLLLDLKDLRAMLQHVGDNAAQFKTQYGNVTGA
                     SIGTIQRGLLALESQGAERFFGEPMLEIADLMQVRGGKGVVNILAADKLMNAPKLYSS
                     FLLWLLAELFEQLPEVGDPEKPKLVFFFDEAHLLFDEAPKALVEKVEQVVRLIRSKGV
                     GVYFVTQNPLDVPESVLGQLGNRVQHALRAFTPRDQKAVKTAAETLRPNPKLKAEKVL
                     TELGVGEALVSLLDEKGRPHVVERAFIVPPASHVGPLSTEERQQIVRGSSLRSKYENT
                     VDRESAYEKLRQQVRQKQAPAVPVEPASAPSPISDILFGKTGPRGGRQTQGALEAATK
                     SAARAIGSELGRQILRGVLGSILGGGRRR"
     gene            1265..2158
                     /locus_tag="VFX94_04435"
     CDS             1265..2158
                     /locus_tag="VFX94_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5767464.1"
                     /translation="MTGTSYRAWGIVFALVGVLAFSFRPILIKLSYGAHPVSPVTLLF
                     LRMMLSAPFFLAIGWWLRNQKPRLERRDWLAVAGLGFIGYYAASFLDFIGLQWVGAGV
                     GRLILYLFPTLVVLLSFLFLKKPPTAREVIALGVSYAGIALVVSSQVSVENRLFALGA
                     AFVFASALCYAIYLVAGSQVVKRVGSMRFTAYSTLVATAPAVLQFFMTESTQSLQLPG
                     AVWGYAIVLATVSTVLPLFLQAEALNRIGANHFALIGAIGPVSVAVTSALGLDEPFTW
                     VQALGGALVICGVLLVSLRKG"
     gene            2161..>2445
                     /locus_tag="VFX94_04440"
     CDS             2161..>2445
                     /locus_tag="VFX94_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5767465.1"
                     /translation="MDLGIRGKTALVCAASKGLGKGCAIALARDGANLVITARSADAL
                     EATAAELRKTFGVNVKTVAGDITTAEGRKAALEAAPAADILVNNAGGPPPG"
BASE COUNT          390 a          809 c          811 g          435 t
ORIGIN      
        1 aggtcctgga aaggctcaac acgctaaagg tgaaagccga gttcgcgggt tgcccggtgg
       61 agttctggga catgtacggc gcgagcggcc tgccggtgcg cgcgacggtt tcggacatgg
      121 gaccgctgct gctcgcccgc atgctcgacc tgaacgagac gcaagccggc gtattgagcc
      181 tggtgttcaa aatcgccgac gaccacgggc tgctgcttct ggacctgaag gacctgcgcg
      241 ccatgctgca gcacgtcggc gacaacgcag cgcagttcaa gacccagtac ggcaacgtca
      301 ccggcgcttc catcggcacc atccagcgcg gcttgctcgc gctggagagc cagggcgccg
      361 agcggttctt cggcgagccg atgctcgaga tcgccgacct catgcaggtc cgcggcggga
      421 aaggcgtcgt caacatcctc gccgctgaca agctcatgaa cgccccgaag ctctattcga
      481 gcttcctcct ctggctgctt gcggagctct tcgagcagct cccggaggtt ggcgaccccg
      541 agaaacccaa gctcgtgttc tttttcgacg aggcccatct gctcttcgac gaagcgccca
      601 aggcgctcgt cgagaaagtt gagcaagtgg tgcgcctcat ccgctcgaag ggcgttggcg
      661 tttacttcgt gacccagaac ccgctcgacg tgcccgaaag cgtgctcgga cagcttggca
      721 accgcgtgca gcacgcgctg cgcgcgttca ccccgcgcga ccagaaggcg gtcaagaccg
      781 cggccgagac gctccgcccc aaccccaagc tcaaggccga gaaggttctc accgagctcg
      841 gtgtaggcga ggcgctggtg agcctgctcg atgagaaggg ccgtccccac gtcgtcgagc
      901 gcgcgttcat cgttcccccc gcgagccatg tcggtccgct gagcactgag gagcgccagc
      961 agatcgtgcg cggttcgagc ctgcgctcca agtacgagaa taccgtcgac cgcgaatccg
     1021 cctacgagaa actgcggcag caagtgcggc agaagcaggc gcctgcggtc ccggtggagc
     1081 ccgcttctgc gccaagcccg atttccgaca tcctgttcgg caagaccggg ccgcgcggcg
     1141 gccggcagac gcaaggtgcg ctcgaggcgg caaccaaaag cgcggcgcgt gcgatcggct
     1201 cggagctcgg ccggcagatc ctgcgcggag tgctcggctc gatcctcggc ggcggccgcc
     1261 gccgttgact ggcacttcct acagggcctg gggcatcgta ttcgctttgg tcggcgtgct
     1321 tgcgttctcg ttccgaccca tcctgatcaa gctttcgtac ggcgcgcatc cggtgagccc
     1381 ggtgacgctg ctgttcctcc gcatgatgct gtcggcacct ttttttctcg cgatcgggtg
     1441 gtggctacgc aatcagaagc cgcggctcga gcggcgcgac tggctggccg tcgcggggct
     1501 aggcttcatc ggctactacg ccgcgagctt cctcgacttc atcggcctgc aatgggtcgg
     1561 cgccggcgtg ggccggctga tcctgtacct ctttcccacg ctggtggtgc ttctgtcatt
     1621 cctgtttctc aagaagcccc cgaccgctcg cgaggtgatc gccctggggg tgagctatgc
     1681 gggcatcgcg ctcgtcgttt cgagccaggt gtcggtcgag aaccggctgt tcgcgctcgg
     1741 cgcagcattc gttttcgcct cggcgctgtg ctacgcgatc tacctcgtcg ccggcagcca
     1801 ggtggtgaag cgggtgggat ccatgcgctt caccgcctac tccacgctcg tcgccacggc
     1861 gcctgcggtc ctccagttct tcatgaccga gtcgacgcag tccctgcagc tccccggcgc
     1921 ggtctggggc tacgccatcg tgctcgccac ggtgagcacg gtcctgcccc tttttctgca
     1981 ggccgaagcg ttgaaccgca tcggcgcgaa ccacttcgct ctgattggag cgatcgggcc
     2041 ggtgagcgtc gcggtcacaa gcgcgctcgg actggatgaa ccgttcacct gggtgcaggc
     2101 gctcggcggc gcactggtga tctgcggcgt gctgctcgtg tcactaagga aaggttgagc
     2161 atggatctcg gaatccgcgg caagaccgcg ctcgtatgcg cggcgtcgaa gggactcggc
     2221 aagggatgcg ccatcgcgct cgcgcgcgac ggtgcgaacc tggtcatcac cgctcgcagt
     2281 gcggacgcac tggaagccac tgcagctgaa ctgcgcaaga ccttcggtgt gaacgtgaag
     2341 accgtcgcgg gggacatcac gacggcggaa ggacgcaagg ccgcgctcga ggcggcgccg
     2401 gcggcggaca tcctggtgaa caacgcgggc ggtccgccgc cgggc
//
LOCUS       DASUNT010000224         2809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_730791, whole genome shotgun sequence.
ACCESSION   DASUNT010000224 DASUNT010000000
VERSION     DASUNT010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730791"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VFX94_04445"
     CDS             complement(<1..584)
                     /locus_tag="VFX94_04445"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5767466.1"
                     /translation="MPGPLEGVRVLDLTRVLAGPWAAQNLADLGAEVIKLERPKKGDD
                     SRAFGPPWLKDAQGRDTSESAYFACANRGKKSLTLDLAHPRGQAIARELAAKCDVLLE
                     NFKFGDLDRYGLGYSQLRAVNPGLIYCSVTGFGHTGPWRERPGYDFMIQGMGGLMSIT
                     GERDDRPGGGPQKAGIPIADLITGMYASIAVCAAL"
     gene            complement(701..1477)
                     /locus_tag="VFX94_04450"
     CDS             complement(701..1477)
                     /locus_tag="VFX94_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEX5767467.1"
                     /translation="MRPLAELDARDVRAVLVDIDDTLSTQGRLTAQAYGALERLQLGG
                     LRVVPVTGRPAGWCDHIARMWPVDAVVGENGAFYFFYSGNRLHKRFCDDPQSRAKKRA
                     RLDAIAAHIIAAVPGCAMASDQPYRETDLAIDYCEDVPPLPLEAASHIAELMRKAGLT
                     AKVSSIHVNGWFGDYDKLAMARRLFAERFAMDLSAANREVVFAGDSPNDSSMFGFFEN
                     SVGVANVRRFESHLSHAPKYVTGTACGAGFAELAEHLLRR"
     gene            complement(1474..>2809)
                     /gene="glpD"
                     /locus_tag="VFX94_04455"
     CDS             complement(1474..>2809)
                     /gene="glpD"
                     /locus_tag="VFX94_04455"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEX5767468.1"
                     /translation="TSSASSKLIHGGLRYLEQFEFRLVAEALAEREILLRIAGHLTWP
                     TRFVAPHLPGLRPRWMIRVGLFLYDHLARRSLLPGSQAVRLDRPPYSSALRPELRHGF
                     VFSDCRVDDARLVVANALDAAQRGARILTRTELISAKRCGDFWNAELSRGEVVQARAL
                     VNAAGPWVKAVLNHNLAQPTRDAVRLVKGSHIVLPRLYEGEHAFILQNDDGRVIFMIP
                     FEDRFTLVGTTDVDYDGDPGTPTASEAEADYLCHALGRYLARPPAPSQALWSYAGVRP
                     LYDDGSADPSAITRDYTLRVDDGAGRAPVLSVFGGKITTYRRLAEQALEKLAPYFPAL
                     KPPWTAQVPLPGSDFESRDAAKRELAEYYPRVPPQVLQGVFRRHGTTAAQVLGDGRLG
                     EDFGAGLTERELAHFMEREWARSAEDVLWRRTKCGLHMTEAQCARVARVVGR"
BASE COUNT          481 a          962 c          909 g          457 t
ORIGIN      
        1 agcgcggcgc agactgcgat gctcgcgtac atcccggtaa tcaggtcggc gatcggaatg
       61 ccggccttct gcggccctcc cccgggacgg tcgtcgcgct cacccgtaat gctcatcagc
      121 ccgcccattc cctggatcat gaagtcgtac cccggccgct cgcgccaagg gcccgtatgc
      181 ccgaaaccgg tcacggagca gtagataagg ccggggttca ccgcgcgcag ctgtgagtag
      241 ccaaggccgt agcggtcgag gtcgccgaac ttgaaattct cgagtaatac atcgcatttc
      301 gccgccagct ctcgcgcgat cgcctggccg cgcggatgcg cgaggtcgag cgtcagcgat
      361 ttcttgcctc ggttggcaca ggcgaagtac gccgattctg aggtgtcgcg gccctgcgcg
      421 tccttgagcc agggcggacc gaacgcgcgc gaatcgtcgc ctttcttggg gcgctcgagc
      481 tttatgacct ccgcgcccag gtcggccagg ttctgcgcgg cccaggggcc cgccagcacg
      541 cgcgtcaggt caagaacgcg aactccttcc aggggccccg gcatcgggcc gcagtctact
      601 tgaccgtgat gttgttgtgc aggctacgca cgccgttcac gaccgcgggc aagccggcac
      661 gctcagcctc gcgtcgcgtg cctatgacgc gagtcgcacc tcaacgacgc agaaggtgct
      721 cggcgagctc cgcgaacccc gcgccgcagg ccgtgcccgt cacgtacttt ggcgcgtggg
      781 agaggtgcga ttcgaaccgc cggacgtttg caaccccgac cgagttttcg aaaaagccga
      841 acatcgacga gtcgttgggc gaatcgccgg cgaacaccac ctcacggttc gcggcgctga
      901 ggtccatagc gaagcgctcg gcgaaaagcc gcctcgccat ggcgagcttg tcgtaatcgc
      961 cgaaccaacc gttgacatgg atcgagctca ccttggcggt aaggcccgct ttgcgcatca
     1021 gctccgcgat gtggctcgcc gcctcgagcg gcagcggcgg cacgtcctcg cagtaatcga
     1081 tcgcgagatc ggtctcccgg taaggctggt cggaagccat cgcgcaaccc ggtaccgcgg
     1141 cgatgatgtg cgcagcgatg gcgtccaggc gtgcgcgctt ttttgcccgg ctctgcggat
     1201 cgtcgcagaa gcgcttgtgc aggcggttgc cgctgtagaa gaagtagaag gcgccgttct
     1261 cgcccacgac ggcatccact ggccacatcc gcgcgatgtg gtcgcaccat ccggccggac
     1321 gcccggtgac cggcaccacc cgaagcccgc cgagctgcaa acgctcgagc gcgccgtaag
     1381 cttgtgcggt aagccggcct tgcgtgctca aggtatcgtc gatatcgacg agcaccgcgc
     1441 gcacgtctcg tgcgtcgagc tccgcgagcg gtctcatcgg ccgaccaccc gcgcgacgcg
     1501 agcgcattgc gcctccgtca tgtgcaggcc gcacttggta cgccgccaga ggacgtcctc
     1561 agccgagcgc gcccactccc gctccatgaa atgcgccagc tcgcgctcgg taagtcccgc
     1621 cccaaagtcc tctccaagcc gcccgtcgcc aagtacctgg gccgcagtcg tcccgtggcg
     1681 cctgaacacc ccttgcaaca cctgcggcgg cactcggggg tagtactcgg ccagctcgcg
     1741 ctttgccgca tctcggctct cgaaatcgct gcccggaagc gggacctgcg ccgtccaggg
     1801 cggcttgagc gccgggaagt atggcgcgag cttctcgagt gcttgctccg caagccgccg
     1861 gtatgtggtg atcttgccgc cgaatactga aagcacgggc gctcgccctg cgccgtcgtc
     1921 cacccgcaac gtatagtcac gagtgatggc tgaagggtcg gccgagccgt cgtcgtaaag
     1981 cggccgcacc ccggcgtagc tccacagcgc ctgcgaaggc gcaggaggac gggcgagata
     2041 gcgcccgagc gcatggcaca ggtagtcggc ctctgcctcg cttgcggttg gcgttcccgg
     2101 gtcgccgtcg tagtcgacgt ccgtggtgcc caccagcgtg aaccgatcct cgaacggaat
     2161 catgaagatg acgcgcccgt cgtcattctg cagtatgaaa gcgtgctccc cttcgtagag
     2221 gcgcggcagc acgatatggc tgcctttcac cagccgcacc gcgtcgcgcg tcggctgtgc
     2281 gagattatgg ttcagcactg ccttgaccca cggacccgct gcattgacta gtgcgcgcgc
     2341 ctgcaccact tctcccctgg agagctccgc attccagaaa tcgccgcaac gcttagccga
     2401 aatgagctcg gttcgcgtca gaatgcgcgc tccgcgctgc gcagcatcca gcgcgttcgc
     2461 cacgaccagg cgtgcgtcgt cgacccggca atccgagaac acgaagccat gcctgagctc
     2521 cgggcgcagc gcagacgaat agggcggacg gtcgagccgc accgcttgtg atccgggcag
     2581 cagcgagcgg cgcgcgagat ggtcgtacag gaaaaggccg acgcggatca tccagcgcgg
     2641 gcggagcccc ggaaggtgcg gcgcgacgaa gcgcgtcggc caggtgaggt gcccggcgat
     2701 gcgcagcagg atctcccgct cggcgagcgc ttcggcgacc aaacggaact cgaactgctc
     2761 gagatagcgc aagccaccgt ggatcagctt cgacgatgcg gacgacgtg
//
LOCUS       DASUNT010000225         9235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1169663, whole genome shotgun sequence.
ACCESSION   DASUNT010000225 DASUNT010000000
VERSION     DASUNT010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9235
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169663"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VFX94_04460"
     CDS             <1..462
                     /locus_tag="VFX94_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5767469.1"
                     /translation="SRFSSFERKESRGRGIGFAKYKNLGAYCAVLAEVEVTHELRVRR
                     LVAAGDVGLAVNPDGVANQIEGGCIQAASWTLKEAYRPGVVGWEDYPILRFSEVPAVE
                     VLLMKNTENPVGAGECAMGPTAAAIANALYDALGVRVRDLPLTPERIAAAM"
     gene            complement(464..811)
                     /locus_tag="VFX94_04465"
     CDS             complement(464..811)
                     /locus_tag="VFX94_04465"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX5767470.1"
                     /translation="MARRKPDAMFVVTDVLTGRNRKRFIEFATTHRIPAMYETSLFVR
                     EGGLMSYGPSFDDGFREAAGYIDRIFKGAKPTDLPAVQPRRYYLAVNLKAAADLGLTM
                     PPSLLLRTDETIE"
     gene            complement(763..1455)
                     /locus_tag="VFX94_04470"
     CDS             complement(763..1455)
                     /locus_tag="VFX94_04470"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5767471.1"
                     /translation="MKTFGVGFLLCVTLGVLATPVPAEAQAPQKVFRVGHLAAPGKTP
                     DGGPPRALVEALRALGYVEGRNVAYEARFAEGKLERLPELAADLVRLKMDVIVAQGGS
                     AIEAARRATTTIPIVVAPAAGDAVAVGWISTLARPGGNITGLSDESVQLSAKRMELLK
                     EAVPKAALIAIVWNDNDHGMTLRYREIEKAARTLRVEVLPLGVRNVEDFTGAFGRWRG
                     ASRTPCSSLRTF"
     gene            complement(1478..2440)
                     /locus_tag="VFX94_04475"
     CDS             complement(1478..2440)
                     /locus_tag="VFX94_04475"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5767472.1"
                     /translation="MKVIALEEHYWDQEVATHFKERGPEMRVPALQERLFDVGALRLK
                     EMDEAGIDLQVLSHGAPATQRIDADTAVPLARRANDRLHQTVEKNKGRFEAFAVLPTA
                     NPRAAADELERSVTKLGFKGGMVHGLTAVDGSLFFLDDKRFWPVFERAQALDVPLYMH
                     PAVPHRAVIEAYYKDYLEQFPGLMTAAWGFTVETATQGIRLVLSGLFEVYPKLKIILG
                     HLGESLPFSLWRIDMALARQGNRPTAFRDTFREHFWITTSGNFCTPALLCCVMEMGVD
                     RILFSVDYPFVPNPPGVKWMEQVPLSTEDRAKILHGNAQRLLKL"
     gene            2457..3221
                     /locus_tag="VFX94_04480"
     CDS             2457..3221
                     /locus_tag="VFX94_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5767473.1"
                     /translation="MIKTRYRIDGAGSRWVTFVTGIANDLTMWDAQVPVLERGLRILR
                     YDLRGHGGSEATAGSYSMKLLVEDLKALLDSLSVRTTSLVGLGLGGAIAQAFAIEHAA
                     KVENLVPCCCRARMVMDFAQLWHKLRGTVQEQGLEAIVEPTVQRWFSDEFKAAHPEVL
                     EKIRTMIRGTTLDGYMGVTAAFLGLDVEERLPRIQARTLYVSGAEDKLGGPPDLMAGL
                     AAKVKGARHVSVPKAAHIANIQNPEGFNRVLGDFLQ"
     gene            3218..4162
                     /locus_tag="VFX94_04485"
     CDS             3218..4162
                     /locus_tag="VFX94_04485"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5767474.1"
                     /translation="MRLLLVLLLFSSGAFAQSYPSKPLRWIVPTGPGSAVDVNARRVA
                     TRLAEALGQPVVVENRPGANSMIGAREAARAAPDGYTLFQAVVNNAINDALAPDPCCV
                     LNDRLVPVTRLYLTPLVMVVHPSVPANTVQEYVALAKAQPNSLTYASGGTGAITQMIG
                     EQLKLLAGIQVREVPYKAIGAEVPDLTGGHVASAFLAPIVIRDMVRAGKLRALAVADR
                     SRMAILPDVPTMAEGGYPIVGLGWNGLFVPAGTPRPIVERLHEETVRVLQLKEIRDDA
                     MALGTLLGGESPEEFAAFVRAEVKTWGKVIKDAGIKAQ"
     gene            4171..5124
                     /locus_tag="VFX94_04490"
     CDS             4171..5124
                     /locus_tag="VFX94_04490"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5767475.1"
                     /translation="MKRFVLLLGIFVASICHAQSWPSKPVRIIVNVAPGGVADVTARV
                     LGARLQETLGQPFPVENRAGGDGYIGFDAVARSDADGTTLLYSPGSSMMIAPHIVNRP
                     DLDPLKVLAPVAATGRVSLYVLVYPERPYKNLAEFLAYARSNPGKLNYGTPGNGTSPH
                     IATEVFAREAKLKLNHVPYKGAGPALKDLLGGQIDFVFDPGVGLAQAKADKLRMIAIA
                     GPSRHPDFSDVPTLEENGIRNVDGGPHFGFYAPSGTPRETIAKMNAEIQKAMQDPTVS
                     ARFKALAVDVAEPMTPDQFAAYVRGEHSRYAKLIPELGIKP"
     gene            complement(5114..5944)
                     /locus_tag="VFX94_04495"
     CDS             complement(5114..5944)
                     /locus_tag="VFX94_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5767476.1"
                     /translation="MRLRSVELEVPDVPRAAEFLEQVWGLAPAGSSGRTRFWRGAGDH
                     PYALSLTEASGPGVTAITFSGDDIDRLGKPDRDFDVPGGGRGFEIAGPEGQVYRFIME
                     TSRAASGPDPDRAIQLAHAVLNSRDVDASERFAVEKLGFKVSDRTAHMRFVRCNRTHH
                     ALAYAKSELASLNHIAFEMRDVEAVMRGIGRLRDAGFDCVWGPGRHGPGNNVFGYFIA
                     PWGGIVEYTAEVSEVGDDYRVGSPEDWKWPPGRIDHWGVSKKDLARTSAAEKVVRFRA
                     "
     gene            complement(5941..6780)
                     /locus_tag="VFX94_04500"
     CDS             complement(5941..6780)
                     /locus_tag="VFX94_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5767477.1"
                     /translation="MRIIDLHCYPGTKEWIACQGPYVEALARYWKREWVGKPEDEVLK
                     EFTEAGVEACLVALDLETTIGTRPVGNDYVYALWKRHPKRIIQCWGALEPGKQNVLDE
                     ARKAVTQYGFIGFHFHPIMQHFSVDDPRCRPMFEEISSLGAAVMIDVGTTGMGAGMPG
                     GHGAIIRHAHPASIDRLAADFPNLKIIMAHPGWPWVDETTAVALHKGNVYWEMSGWAP
                     KYFPGNLKVDIRGRLQDKIMFGSDYPSLPYARILKEWRELGYSDTVMEKIFHQNAERI
                     LGL"
     gene            complement(6767..8386)
                     /locus_tag="VFX94_04505"
     CDS             complement(6767..8386)
                     /locus_tag="VFX94_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5767478.1"
                     /translation="MPLPGVVPFPPELAQRYRDKGYWQDRSLAEEFAACFHQYGERVF
                     LVDGETQYTYAELDAATDRLALNLLELGLKPLDRVVPTLPNCAEFVLLYFALQKIGAI
                     PIAALVSHRFAEISQFVQLSGAAMCVYPERHGEFEFEPMIERVKAASPCLRYCVSLPQ
                     LGELLERKPALQKSELQKIRIYPTDPCIFQLSGGTTGIPKLIPRTHNDYAYNSKTAAP
                     VCGVTRDSVLLVALPIAHNLPLACPGIQGYFFQGGRVVLSPSTRPEEVFKLIERNRVT
                     HVKVVPALLIRLINDPSIRRFDLSSLEVIQSGGQRMQPEVRLKTRELIPSAFVQENFG
                     MSEGMIFFVRHDDPEEVKLETCGRPISPDDEVRLVDDEGREVSDGEVGELTCRGPYTL
                     RGYYGVPEYNAKQFTADGFYCSGDLMRKHPSGNYVVEGRKKDLINRGGEKISAEEIEN
                     LILSHPAVQNVACVPVPDPNLGEKMCACVVLKPGRNLDLPSLVDFLKDNEIAKFKLPE
                     RMLVVADFPVSTFGKVSKKALSELAQKNAHH"
     gene            complement(8386..9144)
                     /locus_tag="VFX94_04510"
     CDS             complement(8386..9144)
                     /locus_tag="VFX94_04510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011694044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5767479.1"
                     /translation="MSSKKAALVTGGATGIGRSAVLALARAGYDVAINYSSSESAAKD
                     AAAQAEKFKVKSLLVKCDVADEAAVRSMADKIEKTFGRLDVLVNNAGTTASWKPRDLE
                     TLSLEEWDRVFAVNVRGLFQVTRACVPLLKEAKGCIVNTASIVGLRPGPQPLPYAASK
                     AAVVNLTKTLAWNLGPDIRVNAVAPGWMEGDWMKRMLKDRYDELMAKRAKATPLKRVV
                     TADDVAETMMTLVQSNRFVTGEVIVIDGGYTSST"
BASE COUNT         1560 a         3002 c         3050 g         1623 t
ORIGIN      
        1 tcgaggttct cttcgtttga aaggaaggaa agccgcggcc gcggcatcgg cttcgccaag
       61 tacaagaacc tcggcgcgta ctgcgcggtg ctcgcggaag tggaagtgac gcacgagctg
      121 cgcgtgcgtc gcctcgtcgc cgcgggggat gtcggcctcg cggtcaaccc cgacggcgtg
      181 gcgaaccaga tcgaaggcgg ctgcattcag gctgcgagct ggacgctcaa ggaagcgtac
      241 cggccgggtg tcgtcggctg ggaggattac ccgattctca ggttcagcga agtcccggca
      301 gtcgaggttc tcttaatgaa aaacacagaa aacccggtcg gtgcaggcga atgcgccatg
      361 ggcccgaccg cggccgcgat cgccaacgcg ctttacgacg cgctcggcgt gcgggtgcgt
      421 gatctgccgc ttactcccga gcgcatcgcc gccgcgatgt aaattactcg atcgtctcgt
      481 cggttcgaag cagcagcgac ggcggcatcg taagccccag atcggctgca gccttcagat
      541 tgaccgcaag gtagtacctt ctcggctgca cggctggcag gtcggtcggc ttcgcgccct
      601 tgaaaatccg gtctatgtag cccgccgctt cccggaagcc gtcgtcaaag cttggcccgt
      661 acgacatcag cccgccctcg cgcacgaaga ggctggtttc gtacatcgcc gggatgcggt
      721 gagtggtcgc gaactcgatg aatcgcttgc gattcctccc tgtcaaaacg tccgtaacga
      781 cgaacatggc gtccggcttg cgccgcgcca tcgcccgaag gcgccggtga aatcctcgac
      841 gttgcgcact ccgagcggta gcacctctac acgcaatgta cgcgcggctt tttcaatctc
      901 gcgatagcgc agcgtcatgc cgtgatcgtt gtcgttccag acgatcgcga tcagggctgc
      961 ctttggcacg gcttccttga gaagctccat gcgcttcgcg ctaagctgga cggattcgtc
     1021 gctcaaccct gtgatgtttc cgccggggcg cgcaagcgtc gagatccatc cgaccgcgac
     1081 tgcgtcgccg gccgcgggcg ccacgacgat cggaatggtc gtggtcgccc gcctcgccgc
     1141 ctcgatcgcc gagcctccct gcgccacgat gacgtccatc ttgagccgga cgagatcggc
     1201 cgcgagctcc ggcagccgct ccagcttgcc ctcggcgaag cgcgcctcgt atgcaacgtt
     1261 cctgccctca acgtagccga gcgcgcgcag cgcttcgacg agcgcgcgcg gcggcccgcc
     1321 gtcgggggtc ttgcccggcg ccgcgaggtg accgacgcga aacaccttct gaggcgcctg
     1381 cgcttcagcc ggcaccggcg tagccaggac gccgagcgtt acgcagagca agaacccgac
     1441 gccaaacgtt ttcatccaac gctccgtttt caagtcgcta gagcttgagg agccgctgcg
     1501 cgttgccgtg caggatcttc gcacggtcct cggtgctgag cggaacctgc tccatccact
     1561 tcacgcccgg cgggttgggg acgaacgggt agtcgacgga gaagaggatg cggtcgacgc
     1621 ccatctccat cacgcagcat aaaagggcgg gcgtgcagaa gttgccgctc gtcgtgatcc
     1681 agaagtgttc gcggaaggtg tcgcggaatg cggtggggcg gttgccctgg cgggcgaggg
     1741 ccatgtcgat gcgccacagc gagaagggaa gcgactcgcc aaggtggccg aggatgatct
     1801 tgagctttgg atagacctcg aacaggccgg acaggaccag ccggatgcct tgcgtggccg
     1861 tctcgacggt gaagccccac gctgcggtca tcagcccggg gaactgctcc aggtagtcct
     1921 tgtaataggc ctcgatgacc gccctgtgcg gaaccgccgg gtgcatatag agcggcacat
     1981 cgagcgcctg ggcgcgctcg aagaccggcc agaagcgctt gtcgtcgaga aagaaaagcg
     2041 acccgtccac cgcggtgagg ccgtgcacca tgccgccctt gaagccgagc tttgtcacgc
     2101 tgcgctccag ctcgtcggcg gcggccctgg ggttcgcggt cggcaggacg gcgaaggctt
     2161 cgaatcttcc tttatttttt tcaacggttt ggtggagccg gtcgttggcg cggcgcgcga
     2221 gcggcaccgc cgtgtcggca tcgatgcgct gcgtggcggg agcgccgtgc gacaggacct
     2281 ggaggtcgat gccggcttcg tccatctcct tcagtcgcag cgcgccgacg tcgaacagcc
     2341 gctcctgcaa cgcgggcacg cgcatctccg gcccgcgctc cttgaaatgc gtggcgactt
     2401 cctgatccca gtagtgctct tcgagggcga tgactttcat ggtgaaatcc taactgatga
     2461 tcaagactcg ttaccgaatc gacggcgcag gttcgcgctg ggtgaccttc gtcaccggaa
     2521 tcgcgaacga cctgacgatg tgggacgccc aggtgccggt gctcgagcgc ggcttgcgca
     2581 tcctgcgcta cgacctgcgc ggccacggcg gcagtgaggc gaccgcgggt tcgtacagca
     2641 tgaagctctt ggtcgaggac ctgaaggcgc tgctcgactc gctcagcgtg cgcacgacct
     2701 cgctcgtcgg cctcggcctc ggcggcgcga tcgcgcaggc gttcgccatc gagcacgctg
     2761 ctaaagttga aaacctggtc ccctgctgct gccgcgcccg catggttatg gacttcgcgc
     2821 agttgtggca caagctgcgc ggcacggtac aggagcaggg gctcgaggcg atcgtcgagc
     2881 cgacggtcca gcgctggttc tccgatgaat tcaaggcagc gcatccggaa gtgctggaga
     2941 agatacgaac gatgatccgc gggacaacgc ttgacggata catgggcgtt accgccgcgt
     3001 ttctcggcct cgacgtggaa gagcgcttgc cgcgaataca agcccgaacg ctctatgtca
     3061 gcggggcgga ggacaagctc ggcggcccgc cggatctcat ggccggcctg gccgcaaagg
     3121 tgaagggcgc acgccacgtc tcggtgccca aggccgcgca catcgctaat atccagaacc
     3181 cggaggggtt caatcgcgtg ctgggggact tcctgcaatg aggcttctgc tcgtcctgct
     3241 gcttttctcg tcgggggcgt tcgctcaaag ctacccgagc aagccgctgc gatggatcgt
     3301 gcccaccggg ccgggcagcg ccgtcgacgt caacgcgcgg cgtgtggcaa cccggcttgc
     3361 cgaggctctc gggcagccgg tggtggtcga gaaccggccg ggcgcgaaca gcatgatcgg
     3421 cgcgcgcgaa gcggcgcgcg cggcgcccga cggctacacg ctcttccagg cggtggtgaa
     3481 caacgcgatc aacgacgcgc tcgcgcccga tccttgctgc gtgctgaacg acaggctcgt
     3541 gccggtgacg cgcctctacc tcacgccgct ggtgatggtc gtccacccct ctgttcccgc
     3601 gaacacggtg caggaatatg ttgcgctcgc gaaagcgcag ccgaactcgc tcacctacgc
     3661 ctccggcgga accggcgcga tcacgcagat gatcggcgaa cagttgaagc tcctcgccgg
     3721 tatccaggtg cgcgaggtgc cgtacaaggc gatcggcgcg gaggtcccgg acctgaccgg
     3781 cggccatgtc gcgagcgcgt ttctcgcccc gatcgtgatc cgcgacatgg tgcgcgctgg
     3841 gaaactgcgc gcgctcgccg tcgccgaccg cagccgcatg gcgatcctgc ccgacgtgcc
     3901 gacgatggcc gagggcggct accccatcgt cgggctcgga tggaacggcc tcttcgtgcc
     3961 ggccggcacg ccgcgcccga tcgtcgagcg cctgcacgag gagacggtgc gcgtgctcca
     4021 gctcaaggag attcgcgacg acgcgatggc gctcggcacg ctgctcggcg gcgagagccc
     4081 ggaggagttt gccgcattcg tgcgcgccga agtcaagaca tggggcaagg tgatcaagga
     4141 cgccggcatc aaggcgcaat aggagcagac atgaagcgtt ttgtattgct gcttgggatc
     4201 ttcgttgcga gcatctgcca tgcgcagagc tggccctcga agccggtgcg catcatcgtc
     4261 aacgttgcgc cgggcggcgt ggcggacgtc accgcgcgcg tgctcggcgc gcggctgcag
     4321 gagacgctcg gccagccgtt cccggtcgag aaccgcgccg gcggcgacgg ctacatcggc
     4381 ttcgacgcgg tggcccgctc ggacgcggac ggcaccacgc tgctctactc gccgggctcg
     4441 agcatgatga tcgcgccgca catcgtgaac cgcccggacc tcgatccgct caaggtgctc
     4501 gcgccggtcg ccgccacggg ccgagtgtcg ctctacgtgc tcgtataccc ggagcggccg
     4561 tacaagaacc tcgccgagtt cctcgcctat gcgcgcagca atccgggcaa gctcaactac
     4621 ggcacgcccg gcaacggcac ctcgccgcac atcgccaccg aggtgttcgc gcgcgaggca
     4681 aagctgaagc tgaaccacgt gccgtacaag ggcgccggcc cggcgctcaa ggacctgctc
     4741 ggcgggcaga tcgatttcgt gttcgacccg ggcgttggct tggcgcaggc aaaagcggac
     4801 aagctgcgca tgatcgcgat cgcgggtccc agccgccacc ccgacttttc cgacgtgccc
     4861 acgctcgagg agaacggcat caggaacgtc gacggcggac cgcacttcgg cttttatgcg
     4921 ccgagcggca cgccgcgcga gaccatcgcg aagatgaatg ccgagatcca gaaggcgatg
     4981 caggacccga cggtgagcgc gcggttcaag gcgctcgcgg tggacgtggc cgagccgatg
     5041 acgccggacc agttcgctgc ctacgtgcgc ggcgaacatt cgcgctacgc gaaactgatc
     5101 cccgagctcg ggatcaagcc ctgaagcgca ccaccttctc cgcggcggaa gtgcgggcga
     5161 gatccttctt cgacacgccc cagtggtcga tgcgccccgg cggccacttc caatcttccg
     5221 gactgccgac ccggtaatcg tcgccgacct cggagacttc cgcggtgtat tcgacgatcc
     5281 cgccccaggg tgcgatgaag tagccgaaca cgttgttccc cgggccgtgg cgccccgggc
     5341 cccagacgca gtcgaagccc gcgtcgcgca gccggccgat gccgcgcatc accgcctcca
     5401 cgtcgcgcat ctcgaaggcg atgtggttga gcgatgcgag ctccgatttc gcgtaggcga
     5461 gcgcgtggtg cgtgcggtta cagcgcacga agcgcatgtg cgcggtgcga tcggatacct
     5521 tgaatccgag cttctcgacg gcgaagcgct cggatgcgtc gacgtcccgg gagttcagca
     5581 ctgcatgcgc gagctggatc gccctgtcgg gatccggacc ggacgcagct cggctcgtct
     5641 ccatgatgaa gcggtaaacc tgcccttcgg gccctgcgat ctcgaacccc ctgccgccgc
     5701 ccggcacgtc gaagtcgcgg tcaggttttc cgagccgatc gatgtcgtcg cccgagaagg
     5761 tgatcgcggt gacgcccggc ccgctcgcct cggtgagcga gagggcgtag ggatggtcgc
     5821 cggcgccacg ccagaatcta gtcctgccgc tgcttccggc gggcgcgagc ccccagacct
     5881 gctccaggaa ctccgccgcc cgcggtacgt ccggtacctc gagctcgacg ctgcgcagcc
     5941 tcacaggccg aggatcctct cggcgttctg gtggaaaatc ttctccatca ctgtgtcgga
     6001 gtagccgagc tcgcgccatt ccttgaggat gcgcgcatac ggcaggctcg ggtagtcgct
     6061 gccgaacatg atcttgtcct gcagccggcc gcggatatcg accttgagat tgccggggaa
     6121 atacttcggc gcccagcccg acatctccca atagacgttt cccttgtgca gcgcgaccgc
     6181 ggtggtttca tcgacccatg gccagcccgg gtgcgccatg atgatcttga ggttgggaaa
     6241 gtcggcggcg aggcgatcga tgctcgccgg atgcgcgtgc cggatgatcg cgccgtgccc
     6301 gcccggcata ccggcgccca tgcccgtagt gccgacgtcg atcatcacgg cggcacccaa
     6361 ggaagaaatc tcttcgaaca tcggccggca gcgcgggtcg tccaccgaga agtgctgcat
     6421 gatcgggtgg aagtgaaagc cgatgaagcc gtattgcgtt acagccttgc gcgcttcatc
     6481 gaggacgttc tgcttgcccg gctcgagcgc gccccagcac tggatgatcc gcttcgggtg
     6541 gcgcttccac agcgcgtata cgtagtcgtt gccgacgggc cgggtcccga tggtggtttc
     6601 gaggtcgagc gccaccaggc acgcttccac gccggcttcg gtgaattcct tcagcacctc
     6661 gtcttcgggc tttccgaccc actcgcgctt ccagtacctg gcgagcgcct cgacatacgg
     6721 gccctggcac gcgatccatt ccttggtgcc cgggtagcag tgcagatcaa tgatgcgcat
     6781 tcttctgggc gagctcggac agtgccttct tggatacctt gccgaacgtg gacaccggga
     6841 agtcggcgac caccagcatt cgctccggca gcttgaactt cgcgatctcg ttgtctttca
     6901 ggaaatcaac caggctcggc aggtcgagat ttcttcccgg cttcaaaacc acgcaagcac
     6961 acatcttctc gccgagattc gggtcgggaa ccggcacgca ggccacgttc tgcaccgccg
     7021 gatgcgaaag aatcaggttc tcgatctcct cggcgctgat tttctcgccg ccgcggttga
     7081 tgaggtcctt cttgcgcccc tcgacgacat agttgcccga aggatgcttg cgcatcaggt
     7141 cgccggagca gtagaagcca tcggcggtga actgcttcgc gttgtactcg ggcacgccgt
     7201 agtagccgcg cagcgtgtac gggccccggc aggtgagctc gccgacctcg ccgtcagaca
     7261 cttcgcgccc ttcgtcgtcc accaggcgca cctcgtcatc cggcgagatc ggccggccgc
     7321 aggtctcgag cttcacttct tccgggtcgt cgtgccgcac gaagaagatc atgccttcgg
     7381 acatgccgaa gttttcctgc acgaaggcgc tcgggatgag ctcgcgcgtc ttcaaccgca
     7441 cctcgggctg catgcgctgc ccgccgcttt ggatcacctc gagcgaggaa agatcgaacc
     7501 ggcggatcga cgggtcgttg atcaggcgga taaggagcgc gggcacgacc ttcacatggg
     7561 ttacccggtt tcgttctatc agcttgaaaa cctcctccgg gcgggtgctc ggcgagagca
     7621 cgaccctgcc gccctggaag aagtaacctt gaattcccgg gcaggctagc ggcagattgt
     7681 gcgcgatcgg cagcgcgact agaagcaccg agtccctcgt cactccgcac accggcgccg
     7741 cggtcttcga gttgtaggca tagtcgttat gcgtgcgcgg gatgagcttc gggatgccgg
     7801 tcgttccgcc cgaaagctgg aagatgcagg gatcggtggg gtagatgcgg attttctgca
     7861 gctcgctctt ctgcagcgcg ggcttgcgct cgagaagctc gccgagctgg ggaagcgaaa
     7921 cgcaatagcg caggcaggga ctggcggcct tgacgcgctc gatcatcggc tcgaactcga
     7981 actcgccgtg ccgctcggga tagacgcaca tcgcggcgcc cgagagctgc acgaactggc
     8041 tgatctcggc gaagcggtga ctgacgagcg ccgcgatcgg gatcgcgccg atcttctgca
     8101 gcgcgaagta aagaaggacg aactccgcgc agttcggcag ggtcggcacg acgcggtcga
     8161 gcggcttcag gccgagctcg agcaggttga gcgccagcct gtcggtggct gcgtcgagct
     8221 cggcgtaggt gtactgcgtc tcgccatcga ccagaaaaac gcgctcgccg tattgatgga
     8281 agcaagccgc gaattcctcg gcaaggctcc tgtcctgcca gtagcccttg tcgcggtaac
     8341 gctgcgcaag ctcgggcggg aaagggacga cgccgggaag aggcatcagg tcgagctggt
     8401 atagccgccg tcgatcacga tgacttcgcc ggtgacgaag cggttggact ggacgagcgt
     8461 catcatggtt tcggcgacgt catcggcggt caccacgcgc ttgaggggcg tggccttggc
     8521 gcgctttgcc atcagctcgt cgtatctgtc cttgagcatg cgcttcatcc agtcgccttc
     8581 catccagccg ggcgcgaccg cgttcacgcg gatgtcgggg ccgagattcc aggcgagcgt
     8641 cttcgtaagg ttcaccaccg cggccttgct tgcggcgtag ggcaggggct gtggcccggg
     8701 gcgcaggccg acgatcgagg cggtgttcac gatgcagcct ttggcttcct tgaggagcgg
     8761 gacgcacgcg cgcgtgacct ggaaaaggcc gcgcacgttg accgcgaaca cccgatccca
     8821 ttcttcgagc gagagcgtct cgaggtcgcg cggcttccag gacgcggtgg tacccgcgtt
     8881 attgacgagc acatccaagc gaccgaaggt cttttcgatc ttgtcggcca tcgagcgaac
     8941 cgcggcctcg tcggcgacgt cgcacttgac gagcaggctc ttgaccttga atttttctgc
     9001 ctgcgccgcc gcgtccttgg ccgccgactc gctcgaggag taattgatcg cgacatcgta
     9061 gccggcgcgc gcgagggcga ggacagcgct gcggccgatc cccgtcgctc cgccggtcac
     9121 cagggcggcc ttcttgctag acatgaacat gttcatgttc agccgcggcc gcggctgtgt
     9181 caagattcgg cgcatgacgg gagcggaatc gctgttgcgc gcattgcgcg cgatg
//
LOCUS       DASUNT010000226         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_643546, whole genome shotgun sequence.
ACCESSION   DASUNT010000226 DASUNT010000000
VERSION     DASUNT010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643546"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VFX94_04515"
     CDS             <1..505
                     /locus_tag="VFX94_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX5767480.1"
                     /translation="FGLIYGMSAFGLAQNLGIERSAAQSYMSKYFARYPGVAAYMERT
                     RNEAHERGFVETVFGRRLWLPDIRSSNQARRQGAERQAINAPMQGTAADLIKLAMIAV
                     QKWIEDAALKTLLIMQVHDELVFEVPEDEVQRVRTTIPELMQSVAKLDVPLVVEVGVG
                     DNWDKAH"
     gene            complement(550..1491)
                     /gene="lipA"
                     /locus_tag="VFX94_04520"
     CDS             complement(550..1491)
                     /gene="lipA"
                     /locus_tag="VFX94_04520"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX5767481.1"
                     /translation="MREAGVKEKGALKTIRIVRREERTAAPLPKPAWIRVRAASPGSR
                     FYDVKRILREQKLHSVCEEASCPNIAECFGKGTATFMILGDICTRRCPFCDVAHGRPL
                     APDAEEPRHLAETIALLGLGYVVITSVDRDDLRDGGAAHFVDCINEVRNASPQTTIEI
                     LVPDFRGRLESSLEILDAAPPDVMNHNLETVPRLYRKARPGGNYPHSLRLLQEFKRRH
                     PSVPTKSGLMVGLGETDEEILTVMRELRAHGVDMLTIGQYLQPSPHHLPVERYVDPSV
                     FAYFAKEAAGLGFRHAAVGALVRSSYHADRQAHQAGV"
     gene            complement(1484..2020)
                     /gene="lipB"
                     /locus_tag="VFX94_04525"
     CDS             complement(1484..2020)
                     /gene="lipB"
                     /locus_tag="VFX94_04525"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX5767482.1"
                     /translation="MRAFTRARDDFSVDELWLLEHSPVYTVGQGAVIPPLANAIPVVK
                     SDRGGDITYHGPGQVVLYTLVDLARRSIKVKRFVWVLEQAVIDLVGHRAKRRPGAPGV
                     YVDGAKIAALGIRVVRGRAYHGLALNVDMDLSPFLAIDPCGYPGLRVTQMRDLGFTDT
                     PQALAERLSRILVETLHA"
BASE COUNT          368 a          659 c          697 g          341 t
ORIGIN      
        1 cttcggcctc atctacggca tgagcgcctt cgggctcgcc cagaacctgg ggatcgagag
       61 gagcgccgcg cagagctaca tgagcaagta cttcgcgcgc tacccggggg tcgcggccta
      121 catggagcgc acgcgcaacg aggcgcacga gcggggcttc gtggagaccg tcttcggccg
      181 gcggctctgg ctgccggaca tccgctcgtc caaccaggcg cgccggcagg gcgccgagcg
      241 ccaggcgatc aacgcgccca tgcagggcac cgccgccgac ctcatcaagc tcgcgatgat
      301 cgcggtgcaa aagtggatcg aggatgcggc cttgaaaacg ttgctcatca tgcaggtcca
      361 cgacgagctc gttttcgagg ttccggaaga tgaggtgcaa agagtcagaa caacgatccc
      421 tgagctcatg cagtcagtcg ccaagctcga cgtgcccctc gtcgtggaag tcggcgtcgg
      481 ggacaactgg gacaaggcgc actagggcga accgaagacc cgccgcagga tcgcctgcgt
      541 gggtggcgct tagactcccg cctggtgcgc ctgccggtcg gcgtggtacg aggagcggac
      601 cagtgcaccg accgcggcat ggcgaaagcc aaggccggcc gcttcctttg cgaagtacgc
      661 aaatactgaa ggatcgacgt agcgctccac gggcaagtga tgcggcgaag gctgcaggta
      721 ttgcccgatg gtcagcatgt ccactccatg cgcgcgcagc tcgcgcatca ctgtgaggat
      781 ctcttcgtcg gtttcgccca ggcccaccat caggcccgac ttggtaggaa ctgaggggtg
      841 gcgccgcttg aactcctgca gcaggcgaag cgaatgcggg tagttcccgc ccggccgagc
      901 cttgcggtag agccggggca ccgtttcgag gttgtggttc atgacgtcgg gcggcgcggc
      961 atccaggatc tcgagcgaac tctccaggcg gccgcggaaa tcgggtacga ggatctcgat
     1021 cgtcgtctgc ggtgaagcgt ttctcacctc gttgatgcaa tccacgaagt gcgccgcgcc
     1081 gccgtcgcgc aggtcgtcgc ggtcgacact ggtaatcacg acgtatccaa ggccgagcaa
     1141 agcgatggtt tctgcgagat gccgcggctc ttcggcgtcg ggagcgagcg ggcggccgtg
     1201 cgctacatcg cagaaggggc agcggcgcgt gcagatatcg cccaggatca tgaaagtcgc
     1261 cgtgcccttg ccgaagcact cggcgatgtt cgggcacgaa gcctcttcgc atacgctgtg
     1321 cagcttctgc tcgcgcagga tgcgcttcac gtcgtagaag cgcgaaccgg ggctcgcggc
     1381 gcgcacgcgg atccaggccg gcttcggcag cggcgccgcc gtgcgctcct cccggcgcac
     1441 gatgcggatc gtcttgagcg cgcctttttc ctttacgccg gcttcacgca tggagcgtct
     1501 ccacgagaat gcgagaaagc cgctcggcga gcgcttgcgg cgtatcggtg aagccgaggt
     1561 ccctcatctg cgtcacgcga agcccgggat agccgcacgg atcgatcgcc aagaatggcg
     1621 agagatccat gtcgacgttg agcgcgaggc cgtggtaagc gcggccgcgc acgacccgga
     1681 tgcccagcgc ggcgatcttg gcgccgtcga catacacgcc cggggcacca ggccttcgtt
     1741 tggcgcggtg cccgacgagg tcgatcaccg cctgctcgag cacccagacg aaccgcttca
     1801 ccttgatcga gcgccgggcg agatcgacca gcgtgtagag cactacctga ccgggtccgt
     1861 ggtaggtgat atcgccgccg cggtcgctct ttaccacggg tattgcattg gcgagcggcg
     1921 gaatgacggc gccttgtccc accgtataga ccggcgaatg ctcgaggagc cagagttcgt
     1981 cgacgctaaa gtcgtcccgc gcacgggtaa acgcgcgcat cgcttcccag gttggctcat
     2041 acgccacgcg gccgaggcgc ttcac
//
LOCUS       DASUNT010000227         3265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_906239, whole genome shotgun sequence.
ACCESSION   DASUNT010000227 DASUNT010000000
VERSION     DASUNT010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906239"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2380
                     /gene="ileS"
                     /locus_tag="VFX94_04530"
     CDS             <1..2380
                     /gene="ileS"
                     /locus_tag="VFX94_04530"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX5767483.1"
                     /translation="IERQKKDFQRLGVLGDWDNPYTTMAPRTEAEEIRVLGRLLEKGY
                     VYRGLKPVNWCFDCGSALAEAEVEYEDRKDPAVDVAFAVSERDVQKLGAAFNVPINKA
                     ACAVVWTTTPWTLPANQALNVHPDFSYDLVDTEKGLLILATELKDVCLKRYGLSGRTL
                     GQCKGRALENLRFRHPFYEREAPVYLGDYVTLETGTGIVHSAPAYGVEDFESCRRYGM
                     RDADILAPVMADGRFAPSLELFGGLSIWDANAKIVKTIEERGALLAVDWKHTHSYMHC
                     WRHKTPVILRATTQWFASMDKAIAGESLRQTALRGIENTQFFPSWGKARLHGMIANRP
                     DWTLSRQRQWGVPMPFFLHRETGALHPRTPRLLEEVAQRVEQAGIEAWQAVDKEAALG
                     PDAKDYDKSRDTLDVWFDSGSTHFTVMRGSHEADTSFPAELYLEGSDQHRGWFHSSLL
                     ISCMLDGIPPYKGLLTHGFVVDGEGRKMSKSKGNVIAPQEVSNRLGAEILRLWVASTD
                     YSGELSISDEILKRVVESYRRVRNTLRFLLANTSDFDFAKHNVAVADMVEIDRYALAM
                     TAAMQAQATADYERYQFHLVAQKLLAFCSEDLGAFYLDVLKDRLYTCGADSRPRRSAQ
                     TALWHITHSLVRLMAPILSFTAEELWQVFTKEESIFLATWHQLPALVDGAELMAKWTR
                     LRQLRDPVRKEIEALRAEGKAGSSLQAEADFGAAGQDYEALASLGDDLKFLLLTSAAR
                     VRKSNQLEVNVRPSAHPKCERCWHYRADVNDEALCGRCQTNLHGPGETRRYA"
     gene            2373..2861
                     /gene="lspA"
                     /locus_tag="VFX94_04535"
     CDS             2373..2861
                     /gene="lspA"
                     /locus_tag="VFX94_04535"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX5767484.1"
                     /translation="MPSAPRWFALSAAIVVADQLAKWAVLDYFAGREPREPITGFLNL
                     VLVCNKGAAFSFLADAAGWQTPLFIVFALAAAVLCGTLIVRNPDKRLFCAGLALVVGG
                     ALGNVIDRFRFGCVVDYLDFYVERWHWPAFNVADSAITLGAALLILDGFAHHEKRAGA
                     PS"
     gene            2893..>3265
                     /gene="ispH"
                     /locus_tag="VFX94_04540"
                     /gene_synonym="lytB"
     CDS             2893..>3265
                     /gene="ispH"
                     /locus_tag="VFX94_04540"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013230.1"
                     /note="catalyzes the conversion of
                     1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into
                     isopentenyl diphosphate (IPP) and dimethylallyl
                     diphosphate (DMAPP); functions in the nonmevalonate
                     isoprenoid biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX5767485.1"
                     /translation="MEVLLASPRGFCAGVERAIETVERALELHGAPIYVRHEIVHNRY
                     VVEDLRAKGAVFVEELDQVPAGATVIFSAHGVSKDVRAHAERRGLKVFDATCPLVTKV
                     HFEVARMLRAGYEVVMIGHRGH"
BASE COUNT          550 a         1083 c         1086 g          546 t
ORIGIN      
        1 gatcgagcgc cagaagaagg atttccagcg cctcggggtg ctcggcgact gggacaatcc
       61 gtacaccacc atggcgccgc gcaccgaggc cgaggagatc cgcgtgctcg gccggctgct
      121 cgaaaagggc tatgtctacc ggggcctgaa gcccgtgaac tggtgcttcg actgcggctc
      181 agccctcgcg gaagccgaag tcgagtacga agatcgcaag gacccggccg tcgatgtcgc
      241 tttcgcggtt tcggagcgcg atgtgcaaaa gcttggagcg gcgttcaacg tgccgatcaa
      301 caaagcagcg tgcgcggtgg tctggaccac gacgccgtgg acgttgcccg ccaaccaggc
      361 gctgaacgtc catcccgatt tcagctatga cctggtcgac acggagaagg ggctgctgat
      421 ccttgccacc gagctgaaag acgtctgcct gaagcgctat ggtctttcgg ggcggacgct
      481 cgggcagtgc aaaggccgag cgctggagaa ccttcgcttc cgacatccgt tctacgagcg
      541 cgaggcgccg gtctatctcg gcgactacgt aacgctcgaa accggcaccg gcatcgtgca
      601 ctcggcgcca gcctacggcg tcgaggattt cgaatcgtgc cgccgctacg gcatgcgcga
      661 cgcggacatc ctggcgccgg taatggccga cggccgcttc gcgccatcgc tcgagctttt
      721 cggtgggctc tcgatctggg acgccaacgc gaagatcgtg aagacgatcg aggagcgagg
      781 cgcgcttttg gcggtggact ggaagcacac gcacagctac atgcattgct ggcgccacaa
      841 gacgccggtg atcctgcgcg ccaccacgca atggttcgcc tcgatggaca aggcgattgc
      901 gggagaaagc ctgcggcaga ccgccctgcg cggcatcgag aacacgcagt tctttccgtc
      961 ctggggcaag gcgcgcctgc acggcatgat cgccaaccgg cccgactgga cgctgtcgcg
     1021 ccagcgccag tggggcgtgc cgatgccttt cttcctgcat cgcgagaccg gcgcgctgca
     1081 tccgcgcacg ccgcggctcc tcgaagaggt cgcgcagcgg gtggagcagg caggcatcga
     1141 ggcgtggcag gccgtcgaca aggaggcggc gctcgggccg gacgcgaagg actacgacaa
     1201 gagccgcgac acgctcgatg tctggttcga ttcgggctcg acccacttca ccgtaatgcg
     1261 cggctcgcac gaggcggaca cgagctttcc ggccgagctc tacctcgaag gctcggacca
     1321 gcaccgcggc tggttccatt cgtcgctgct catctcctgc atgctcgacg ggatcccgcc
     1381 gtacaagggc cttctgacgc acggcttcgt cgtcgacggc gaaggccgca agatgtcgaa
     1441 gtcgaagggc aacgtgatcg cgccgcagga agtctcgaac cggctcggcg ccgaaatcct
     1501 gcgcctgtgg gtggcgagca cggactattc cggcgagctc tccatctccg acgagatcct
     1561 gaagcgcgtg gtggaaagct atcgccgcgt gcgcaacacc ctgcgcttcc tgctcgcgaa
     1621 cacctcggac ttcgatttcg cgaagcacaa cgtcgcggtc gccgacatgg tcgagatcga
     1681 ccgctacgcg ctcgccatga cggcagcgat gcaggcgcag gcgaccgcgg attacgagcg
     1741 ctatcagttc cacctcgtcg cgcagaagct gctcgcgttc tgctccgagg acctcggcgc
     1801 cttctacctc gacgttctga aggaccggct ctatacgtgc ggcgccgact cgaggccgcg
     1861 gcgctcggcg cagaccgcgc tttggcacat cacgcacagc ctggtgcggc tgatggcgcc
     1921 cattctttcg ttcaccgccg aggagctgtg gcaggtgttc accaaggaag aaagtatttt
     1981 ccttgccacc tggcaccagc tgcccgccct cgtggacggc gccgagctca tggccaagtg
     2041 gacgcggctg cgccagctgc gcgatccggt gcgcaaggag atcgaggcgc tgcgcgcgga
     2101 aggcaaggcc ggctcgtcgc tccaggcgga ggccgacttc ggcgccgcgg gccaggatta
     2161 cgaggcgctc gccagcctgg gcgacgacct caagttcctg ctcctgacgt cggcagcccg
     2221 cgtgcgcaaa tcgaatcaat tggaagtcaa cgtgaggccg agcgcgcacc cgaaatgcga
     2281 gaggtgctgg cactaccgcg ccgacgtcaa cgacgaggcg ctgtgcggcc gctgccagac
     2341 taacctgcac ggaccgggcg agactcggcg ctatgcctag cgcgccgcgc tggttcgcgc
     2401 tgtcggcggc gatcgtggtc gccgaccagc tcgcgaagtg ggccgtgctc gattacttcg
     2461 ccggccgcga gccgcgagag ccgatcaccg gcttcctgaa cctggtgctc gtctgcaaca
     2521 agggcgcggc gttcagcttt ctcgccgatg ccgcgggctg gcagaccccg cttttcatcg
     2581 tctttgcgct cgccgcggcc gtgctttgcg gcaccctgat cgtgcgcaac ccggacaagc
     2641 ggctcttctg cgccggcctc gcgctcgtgg tcggcggcgc gctcggcaac gtcatcgacc
     2701 gcttccgctt cggctgcgtg gtggactatc tcgacttcta tgtcgagcgc tggcactggc
     2761 cggcgttcaa cgtggccgac tccgcgatca cgctcggggc ggcccttctc atcctcgatg
     2821 gctttgcgca ccacgaaaag cgagctggag ccccttccta agatctcgcc ttcggcgtgc
     2881 gaaaatagcg cgatggaagt cctgctcgcg agcccgagag gtttctgcgc cggcgtggag
     2941 cgcgccatcg agacggtgga acgcgcgctc gagctgcacg gcgcccccat ctacgtgcgc
     3001 cacgagatcg tacacaaccg ctacgtggtc gaggacctgc gcgccaaggg tgcggtcttc
     3061 gtcgaggagc tggaccaggt gcctgcaggc gcgacggtca tcttcagcgc gcacggcgtg
     3121 tcgaaggacg tgcgcgcgca cgctgagcgc cggggcctca aggtgttcga cgccacctgt
     3181 ccgctcgtca ccaaggtgca cttcgaggtg gcgcgcatgc tgcgcgcggg ttacgaggtg
     3241 gtcatgatcg gccaccgcgg acatc
//
LOCUS       DASUNT010000228         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_118124, whole genome shotgun sequence.
ACCESSION   DASUNT010000228 DASUNT010000000
VERSION     DASUNT010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118124"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VFX94_04545"
     CDS             complement(<1..878)
                     /locus_tag="VFX94_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEX5767486.1"
                     /translation="MELPRTYNAAVDLVERNLAAGRGGKSAYIDDSGATTYAQLAERV
                     DRAASALRGLGLKREQRIGIAMLDAADWVALFLGAIKAGIVPVALNTLLTPKDYEYQL
                     NDSRARALFFSSVLQKNFESIQAKCPHLKYVINDAQLKDLLATADAKAEAAPTTRDEM
                     CFWLYSSGSTGAPKGTVHLHSHLIATADLYAKPVLGIRESDVVFSAAKLFFAYGLGNS
                     LTFPLAVGATTVLMGERPTPDSVFKRLVDKKPTIFYGVPTLYAALLASPNFPKKDQLA
                     MRMCTSAGEALPPEIG"
     gene            complement(845..1504)
                     /locus_tag="VFX94_04550"
     CDS             complement(845..1504)
                     /locus_tag="VFX94_04550"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5767487.1"
                     /translation="MRTRRLFRHGLRPQLLVFEAVARLGSVTRAAEELHLAQPTVSLQ
                     LKKLAETLEVQLFEQRGRKLHLTGAGYALREACDELIHCLLRVDARLEAWRRPKQQVL
                     RLAAEPESRAVAAQLLADFCSRHPGVQGSLHVDERAELLARLSAGTDDVYFLALEIDG
                     VAEERRWSLAHPKGRQLDPAAALFLREALQLDAGDGSANNLPATEEEGQWNSRGPTTR
                     Q"
     gene            1740..>2080
                     /locus_tag="VFX94_04555"
     CDS             1740..>2080
                     /locus_tag="VFX94_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX5767488.1"
                     /translation="MEIFLAPEFWSALAAIIVIDLVLAGDNAIVIALAARNLPRTLQR
                     RAVIWGTVGAVAVRASLTVVVVWLLGIPGLMLAGGILLAWIAYRLLTGDEDGRDHDVP
                     AARSFWAAMRT"
BASE COUNT          330 a          675 c          720 g          355 t
ORIGIN      
        1 ttgccgatct cggggggaag cgcttcaccc gcggaagtgc acatgcgcat tgcgagttgg
       61 tcttttttcg ggaagttcgg cgaagcgagg agtgccgcat acagggtcgg cacgccgtag
      121 aaaatggtcg gcttcttatc aaccaggcgc ttgaaaaccg agtcgggcgt cgggcgctcg
      181 cccatcagga ccgtggtggc gccgaccgcg agcgggaagg taagcgagtt gcccagaccg
      241 taggcgaaga agagcttcgc ggccgagaac acgacgtcgg attcgcggat gccgagcacc
      301 ggcttggcgt agagatccgc cgtggcgatg aggtgggaat gaaggtgcac cgtgcccttc
      361 ggcgcgccgg tcgagcctga ggagtagagc caaaaacaca tctcgtcgcg agtggtcggc
      421 gcggcttcgg ccttcgcgtc cgcggttgcg agcaggtcct tgagctgcgc gtcgttgatg
      481 acgtacttga gatgcgggca cttcgcctgg atgctctcga aattcttctg cagtacagac
      541 gagaagaaca aggcgcgggc ccggctgtcg ttcagctggt actcgtagtc ctttggagtg
      601 agcagcgtgt tcagtgccac cggaacgatc ccggccttga tcgcgccgag gaaaagcgcg
      661 acccagtccg ccgcatcgag catcgcgatg ccgatgcgct gctcgcgctt gaggccgagc
      721 ccgcgcagcg cgctcgcggc gcgatcgacc cgctccgcga gctgggcgta agtggtggcg
      781 cccgaatcgt cgatgtaggc gctcttgccg ccgcgcccgg cggcgaggtt gcgctcgacc
      841 agatctactg ccgcgttgta ggtccgcggg agttccattg accctcctct tcggtggcag
      901 gaagattgtt cgcgctgcca tcgccagcgt caagttgcag cgcctcgcgc aggaagagcg
      961 ccgcagccgg gtcgagctgg cgtcccttgg ggtgcgccag gctccagcgc cgctcctcgg
     1021 ccacaccgtc gatttcgagg gcgaggaagt agacgtcgtc ggtccccgcg ctcagccgcg
     1081 cgagcagctc ggcgcgctcg tcgacgtgca ggctgccctg cacgccgggg tgccgggagc
     1141 agaagtcagc cagcagctgc gccgcgacgg cgcgcgactc gggctcggcc gcaagccgca
     1201 ggacctgctg cttcggccgg cgccaggcct ccaggcgcgc gtcgacgcgc agcaggcaat
     1261 gtatgagctc gtcgcacgcc tcgcgcagtg cgtagcccgc gccggtgaga tggagcttgc
     1321 gcccgcgctg ctcgaagagc tggacctcga gcgtctcggc cagcttcttg agctgcagcg
     1381 ataccgtggg ttgcgcgaga tgcagctcct cggcagcgcg cgtaacactg ccgaggcgcg
     1441 cgaccgcctc gaaaaccagg agctggggcc gcagcccatg tcggaacaaa cggcgcgtgc
     1501 gcacgcgaga catctcggct ccttgcggta accgaggtct ggcttgcagc gcgggatcgg
     1561 gctgccggga tgaccggggg gatattctcc ccgaattgtc ggccatcccg cgggaagcta
     1621 ctcccctgcg gggaacaagc attgtaagtg aacttcgcgg cggatccatt gcggagcgca
     1681 atgaattcca tcgccgaacg tcaattttca tctactgcgg cgccgcacag actcggggca
     1741 tggagatttt cctcgccccc gagttctggt ccgcgctcgc ggccatcatc gtcatagatc
     1801 ttgtcctcgc cggcgacaac gcgatcgtga tcgcgctcgc cgcgcggaac ctccccagga
     1861 cgctccagcg ccgcgccgtc atctggggca cggtcggggc ggtggcggtg cgcgcgagcc
     1921 ttaccgtcgt cgtggtctgg ctgctcggaa tccccgggct gatgctcgcc ggcggcatcc
     1981 ttctcgcctg gatcgcctac cgcctgctta ccggcgacga ggacggccgc gaccatgacg
     2041 ttccggccgc gcgcagcttc tgggcggcga tgcgcaccat
//
LOCUS       DASUNT010000229         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_206520, whole genome shotgun sequence.
ACCESSION   DASUNT010000229 DASUNT010000000
VERSION     DASUNT010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206520"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..1381
                     /locus_tag="VFX94_04560"
     CDS             20..1381
                     /locus_tag="VFX94_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX5767489.1"
                     /translation="MVVLLVGCATKLPEIEPQKLPEVPAEFKEGWTIAAPAEAQPRGE
                     WWKTFNDPVLNELVERADRANANIQVAAARLAQARAVARIVDADRALQASLGASARRA
                     RGIIGGDAGPARNLFATGVDLSYEVDLFGRLSHASAAAVLDAEASAALLQSTRLLVQS
                     EVAQTYLLLRALDAERIIVQETLTAYRVTLSLTERRWRAGDVAELDVARASTEVSATE
                     SEALALERRRAELEHALAILLGLAPSEFALQSEEWRTALPLIPPGIPSTLLTRRPDVT
                     AAQNSLLAAQARVGVAKAAYFPNITLTASGGYASTEIEDLFQWSSRAWLIGAIFSLPI
                     LDGGRREAGVQATSAGLEGAVASYRDQVLVAFREVEDQLSSLRLLAEQAEAQARAVNS
                     ARRTTLLSDARYRGGYVSQLELLDAQRSELRNRREELQVRSARYQSTVTLVRALGGGW
                     DMQ"
     gene            1378..1854
                     /locus_tag="VFX94_04565"
     CDS             1378..1854
                     /locus_tag="VFX94_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767490.1"
                     /translation="MNILTVNLVLSTFVFWVAARIYVVPNLARWSPQAVLVPILLLHS
                     FRHLGLMFLSPGGIYPGMPPQFANPAAYGDLLAAALAMAAILAGTPSWLVWVFNVVGT
                     VDLFTAITLATAYGASEFMGPAYWIPAFWVPALLVTHLLGFIVLLRHPKSPALSAG"
     gene            1961..>2166
                     /locus_tag="VFX94_04570"
     CDS             1961..>2166
                     /locus_tag="VFX94_04570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5767491.1"
                     /translation="MPNRVSLRELLGRAIASCNRENRPLALLMLSIAHLREIKQILGY
                     REGDKLVQEVARRITKELLSADIVA"
BASE COUNT          333 a          762 c          709 g          362 t
ORIGIN      
        1 aagggtcctc accccttttc tggttgtatt gctcgtaggc tgcgccacca agctgccgga
       61 gatcgagccg caaaagctgc cggaggtccc ggccgagttc aaggaaggct ggaccatcgc
      121 ggcgccggcc gaggcgcagc cgcgcggcga atggtggaag acgttcaacg acccggtgct
      181 gaacgagctc gtcgagcgcg ccgaccgcgc caatgccaac attcaagtcg cggcggcgcg
      241 cctcgcacag gcgcgcgccg tggcgcggat cgtcgacgcc gaccgcgcgc tgcaggcgtc
      301 cctcggcgcg agcgcgcggc gcgcgcgcgg catcatcggc ggcgacgccg ggccggcgcg
      361 caatctcttc gccacgggcg tcgacctctc ctacgaggtg gatctcttcg gccggctttc
      421 gcatgcgagc gccgccgccg ttctcgacgc ggaggcgagc gcggcgctcc tgcagagcac
      481 acgcctcctc gtccagtccg aggtagcgca gacctacctg ctgcttcgcg cgctggacgc
      541 ggagcggatc atcgtgcagg aaacgcttac cgcgtaccgc gtaaccctgt ccctgaccga
      601 acggcgctgg cgcgcgggcg acgtcgcgga gctcgacgtc gcgcgcgcga gcaccgaggt
      661 ctcggcgacc gagtccgagg cgctcgccct cgagcggcgc cgcgccgagc tcgagcacgc
      721 gctcgcgatc ctgctcggtt tagccccatc agaattcgca ttacaaagtg aagagtggcg
      781 aactgcgctg cccctcattc cgcccggcat tccgagcacc ctgctcacgc gcaggcccga
      841 tgtcaccgcg gcgcagaact cgctcctcgc cgcgcaagcg cgcgtcggcg tcgccaaggc
      901 ggcgtatttc cccaacatca cgctcaccgc gagcggcggc tacgcctcca ccgagatcga
      961 ggatcttttc cagtggtcct cgcgcgcctg gctgatcggc gcgatcttct cgctgccgat
     1021 cctggacggt ggccggcgcg aggcgggcgt ccaggcgacg agcgccggcc tcgaaggcgc
     1081 ggtggctagc taccgcgacc aagtgctggt ggcgttcagg gaagtcgagg accagctctc
     1141 ttcgcttcgc ctcctcgccg agcaggcgga ggcgcaggcg cgcgcggtga acagcgcgcg
     1201 ccgcacgacg ctgctctccg acgcgcgcta ccgcggcggc tacgtgagcc agctcgagct
     1261 gctcgatgcg cagcgcagcg agctgcgcaa ccggcgcgag gagctgcagg tacgctcggc
     1321 gcgctaccag tccaccgtca cgctggtgag ggcgctcggc ggaggttggg acatgcaatg
     1381 aacatcctta ccgtcaacct cgtcctgagc actttcgtgt tctgggttgc cgcccgaatt
     1441 tacgtcgtgc cgaatctcgc ccggtggagc ccgcaggcgg tgctcgtgcc gatcctgctc
     1501 ctgcactcgt tccgccacct cggcctgatg ttcctttccc ccggcggcat ctacccgggc
     1561 atgccgcccc agttcgccaa ccccgccgcc tacggcgacc tgctcgcggc ggcgcttgcg
     1621 atggcggcga tcctcgccgg cacgccaagc tggctcgtct gggtattcaa cgtggtgggt
     1681 accgtggatt tgttcaccgc cattaccctc gcgacggcgt acggagcgag cgagttcatg
     1741 gggccggcgt actggattcc cgcattctgg gtcccggcgc tgctcgtcac gcatctcctc
     1801 ggcttcatcg tcctcctgag gcatccgaag tccccagctc tttccgcagg ctgacagcct
     1861 gctgtgcgaa acggaatcct agaatcccgg cgcgcggacg tacatttgtt acccaatgcc
     1921 aatagcgaaa gatccggcag atgacgatgc cctgacgcgc ttgccgaacc gcgtgtcgct
     1981 gcgcgagctt ctcggccggg cgatcgcgag ctgcaaccgg gaaaacaggc cgctggcgtt
     2041 gttgatgttg agcatcgctc atctgcgcga gatcaagcag atattgggct accgcgaagg
     2101 cgacaagctc gttcaggaag ttgcgaggcg gatcaccaaa gagctgcttt ccgcggatat
     2161 cgtcgc
//
LOCUS       DASUNT010000230         4375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_820283, whole genome shotgun sequence.
ACCESSION   DASUNT010000230 DASUNT010000000
VERSION     DASUNT010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820283"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VFX94_04575"
     CDS             <1..518
                     /locus_tag="VFX94_04575"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="catalyzes the reduction of adenosine
                     5'-phosphosulfate to AMP and sulfite; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylylsulfate reductase subunit alpha"
                     /protein_id="HEX5767492.1"
                     /translation="GREAPQVGEKEYRDLERTIYQPLENFRIGRNEIVAGTVSPSYLL
                     PIHGLQRLEKIMDEYVGGISTNYMTNGKLLERGLELLHMLKEDMDHMGAEDLHQLQRT
                     WELHHRLLASLSVTHHTMFREETRWPGYYYRGDHLRLDDENWHCFTLSRYDRASGQFA
                     MEKAPVYHIID"
     gene            578..1513
                     /locus_tag="VFX94_04580"
     CDS             578..1513
                     /locus_tag="VFX94_04580"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5767493.1"
                     /translation="MADRRLQVFHAVAKHLSFTKAAETLFMTQPAVTFQVKQLEEELN
                     TRLFDRAQGRIALTPAGQLAFEYAERILDLSAELETRLKELSGLAAGPLLIGASMTIG
                     EYVLPQLIGQFKARFPAVVPTLFVGNSEAVQERVAERTLDLGFIESGSPLATLQSEVC
                     CEDELQVVCAPSHPLAKEPFASPASLTQHAYISREAGSGTRLVVDRYLQQVGVSPELM
                     NRVVELGSPEAIKGLVATGMGFAIMSRVIAAKELQLGHLVQVALRPPLLRNFSVVYAK
                     ERFHSKLVAGFVAFAKSELSAAPKALRPQAELQPG"
     gene            complement(1473..1835)
                     /locus_tag="VFX94_04585"
     CDS             complement(1473..1835)
                     /locus_tag="VFX94_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767494.1"
                     /translation="MREHEIYARWLAAGTRIAFAVLLATFLVYMLGLLEPLVSPRALA
                     RLWALPADRFVAATGAPTGWRWLGFLGKGDYLNFIGVAALASISIVCYARMIPTLPKL
                     QARLAAIQVAVLLVAAVL"
     gene            complement(1835..2674)
                     /locus_tag="VFX94_04590"
     CDS             complement(1835..2674)
                     /locus_tag="VFX94_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5767495.1"
                     /translation="MEWWIWPIALFVATFAMGIVAVLAGVGGGVLFVPIVSAFFPFHL
                     DFVRAAGLMVALTSALSAAPTLLSNGLANLRLAMPLALVGSITSIVGALVGLALPTQA
                     VQVALGLAILAIALVMWRSGRSEHPHVRRPDALSSALGMHGVYHDPALGREVGWQVHR
                     TPLGLVVFGGIGVLAGLFGLGAGWANVPALNLLMGAPLKLSVGTSGFILSVVDTSAAW
                     VYLNRGAVLPIVVVPSLIGVMLGARVGARLLPRTPAHIVKRLVLAVLVLAGARSLLKG
                     FGI"
     gene            complement(2674..3822)
                     /gene="sat"
                     /locus_tag="VFX94_04595"
     CDS             complement(2674..3822)
                     /gene="sat"
                     /locus_tag="VFX94_04595"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX5767496.1"
                     /translation="MRNLVAPHGRAGLKPLALAGQALEEERSRASRLPHLPISSRERG
                     DLIMLGIGGFTPLEGFMTHADWEGVCARYQTASGVFWPIPITLSSHEPVSGEVALIDA
                     ENGETLATMNVVESYKIDKQRECQAVFRTTDAAHPGVAMVMGQSDYNLAGPVKVLSEG
                     GFRAKYGALFMTPAQTRAEFERLGWSRVAAFQTRNPMQRAHEYLVKTAIEVCDGVLVH
                     SLLGNLKPGDIPAEVRARAIAVLIQEYFRPGTVVQAGYPLDMRYAGPREALLHALFRQ
                     NYGCSHLIVGRDHAGVGKYYGPFDAHRIFDEIPAHALEIQPLRMDVTFWCYRCHGMAS
                     GRTCPHEVKDQLQVSGTQLRKWLEEGAEVPPEFSRPEVLEVLREYYKR"
     gene            complement(3822..>4375)
                     /locus_tag="VFX94_04600"
     CDS             complement(3822..>4375)
                     /locus_tag="VFX94_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX5767497.1"
                     /translation="RDGLEFFSSSREVEKLAASVLDSGDVYLVPAFTGLGAPYWDADA
                     RGAMVGLTRGTTKAHIARAALESIAFQSAELLEAMQKDSAQPLAELRVDGGAAANDLL
                     MQFQADLLGVPVVRPRVLETTALGAAYLAGLTVNLWRSREELASHWKAEKRFEPRMPG
                     SERESRMARWREAVTRARGWARP"
BASE COUNT          823 a         1447 c         1420 g          685 t
ORIGIN      
        1 agggcaggga ggccccgcag gtgggcgaga aggagtatcg agacctagaa agaacgatct
       61 accagccgct cgaaaacttc agaatcggcc gcaacgagat cgtcgccggc acggtgtcgc
      121 cgagctacct gctgccgatc cacggcctgc agcgcctgga aaagatcatg gacgagtatg
      181 tcggcggcat cagcaccaac tacatgacga acggcaagct tctcgagcgc ggcctcgagc
      241 tcctccacat gttgaaggaa gacatggacc acatgggcgc cgaagacctg caccagctgc
      301 agcgcacctg ggagctgcat caccggctgc tcgcatcgct ctcagtcacg caccacacca
      361 tgttccgcga ggagacccgc tggccgggct actactaccg cggcgatcac ctgaggctcg
      421 acgacgagaa ctggcactgc ttcaccctct cgcgctacga ccgcgcgagc ggacagttcg
      481 ccatggaaaa ggcgccggtc taccacatca tcgactgaga aaaggggtca ggaccctttt
      541 tttcgtaaaa ggggccggac tcctctaaga tcggccgatg gccgatcgcc gtctgcaggt
      601 ttttcatgct gtcgcgaagc atctctcctt tactaaggcc gccgagacgc tgttcatgac
      661 gcagccggcg gtcacgttcc aagtcaagca gctcgaagag gagctgaaca cgcggctttt
      721 cgaccgggcg caaggccgca tcgcgcttac gccggccggg cagcttgcat tcgaatacgc
      781 ggagcgcatc ctcgatcttt cggcggagct cgagacacgc ctcaaggaac tgagcggcct
      841 tgccgccggg ccgcttctca tcggggcgag catgacgatc ggcgagtacg tgctgccgca
      901 gctcatcggg cagttcaagg cgcgttttcc cgctgtcgtg cctacgctct tcgtcggtaa
      961 ttcggaagcg gtacaggagc gggttgcgga gcgtacgctc gaccttggct tcatcgagag
     1021 cggctcgccg ctcgccacgc tgcaaagcga agtgtgctgc gaggacgagc tccaggtcgt
     1081 gtgcgcgcct tcgcacccgc tcgcaaaaga gccgtttgca tcacccgcat cgctgacgca
     1141 gcacgcctac atcagccgcg aggcggggtc cggcacgcgc cttgtggtcg atcgctacct
     1201 tcagcaggtc ggcgtgtccc cggaattgat gaaccgcgtg gtggagctgg gcagtccgga
     1261 ggcgatcaaa ggactcgtgg cgaccgggat gggcttcgcc atcatgtcgc gcgtcatcgc
     1321 ggcgaaggag ctgcagttgg gccacctggt gcaggtggcg ctgcgcccgc cgcttcttcg
     1381 caacttctct gtggtctacg cgaaggagcg ttttcactct aagctcgtcg ccggcttcgt
     1441 cgccttcgcg aaaagcgagc tctcggccgc gcctaaagca ctgcggccac aagcagaact
     1501 gcaacctgga tagcggcgag ccgcgcctgc agcttcggta gcgttggaat catccgggcg
     1561 tagcagacga tgctgatgct ggcgagcgct gcgacgccga tgaaattcag gtagtcgcct
     1621 ttcccgagaa aaccaagcca tcgccagccg gtgggcgcgc cggtcgcagc cacaaacctg
     1681 tccgccggca gagcccaaag tcgcgcgagc gcgcgcggcg acacgagcgg ttctaacagc
     1741 cccaacatgt acaccaggaa ggtcgccaaa agcacggcaa acgcaatgcg tgttccagcg
     1801 gcaagccagc gcgcgtagat ctcgtgctcg cgcatcagat gccgaagcct ttcagaagcg
     1861 agcgcgcgcc ggcgaggacg agcaccgcga gcacgaggcg cttcacgatg tgcgccggcg
     1921 tgcgcggcaa gagccgcgca ccgacgcgcg cgccgagcat gacaccgatg agcgaaggca
     1981 ccacgacgat agggagcact gcgccgcgat tgaggtacac ccaggccgcc gaggtatcca
     2041 cgactgaaag gatgaacccg gaagttccga ccgacagctt gaggggcgcg cccatcagga
     2101 gattgagcgc cggcacgttg gcccatcctg cgccgaggcc gaaaagaccg gcgagaacgc
     2161 cgatcccgcc gaagaccacc agcccgagcg gcgtgcgatg cacctgccaa cccacctcgc
     2221 gcccgagcgc tgggtcatgg tagacgccat gcatgccgag cgcgctgctg agcgcatccg
     2281 gacggcggac gtgcggatgc tccgatcggc ccgagcgcca catgacgagc gcgatggcaa
     2341 ggatggcgag gccaagcgcc acctgcactg cctgcgttgg caatgcgagg ccgaccagtg
     2401 cgccaacgat ggatgtaatc gagcccacca gcgcgagcgg catggcaaga cgaaggttgg
     2461 cgagtccgtt ggaaagaagc gtgggcgcag cgctgagtgc gctggtaagc gcgaccatca
     2521 gcccggccgc gcgcacgaag tcgagatgga acggaaagaa tgcgctcacg atcggtacga
     2581 acagcacgcc gccgcccacg ccggcgagca ccgcgacaat gcccatggca aacgtcgcca
     2641 cgaagagcgc gatcggccag atccaccact ccattagcgc ttgtaatact cgcgcaggac
     2701 ctcgagcacc tcgggcctgg agaattccgg cggtacttcg gcgccttcct cgagccactt
     2761 gcgaagctgc gtgcccgaga cctggagctg atccttcacc tcgtgcgggc aggtgcgccc
     2821 cgacgccatg ccgtggcagc ggtagcacca gaaagtcacg tccatgcgca gcggctggat
     2881 ctcgagtgcg tgcgccggga tctcatcgaa gatgcggtgc gcgtcgaacg gtccgtagta
     2941 ctttcccacg ccggcgtggt cgcgtcccac gatgagatgc gagcagccgt agttctgccg
     3001 aaagagcgcg tgcagcagcg cctcgcgcgg accggcgtag cgcatgtcca gtgggtagcc
     3061 ggcctgcacc acggtgccgg ggcgaaaata ttcttggata agcacggcga tggcgcgcgc
     3121 gcgcacctcc gccggaatgt cgcccggctt cagattgccg agcagtgagt gcacgagcac
     3181 gccgtcgcac acctcgatcg ccgtcttcac gaggtactcg tgcgcgcgct gcatcgggtt
     3241 acgcgtctgg aatgccgcca cgcgcgacca gccaagccgc tcgaactccg cgcgcgtttg
     3301 cgcgggcgtc atgaagagcg cgccatactt ggctctgaat ccgccctcgg aaaggacctt
     3361 taccggaccg gcgaggttgt aatcgctttg gcccatgacc atggcgacgc cggggtgcgc
     3421 cgcatcggtg gtgcgaaaca ccgcctggca ttcgcgctgc ttgtcgatct tgtagctctc
     3481 gacaacattc atggtggcga gcgtctcgcc gttctccgca tcgatgagcg cgacttcgcc
     3541 gctgacgggt tcgtgcgaag acagcgtgat gggaatcggc cagaagacgc cggaggctgt
     3601 ctgatagcgc gcgcagaccc cttcccagtc cgcgtgcgtc atgaagccct cgagcggcgt
     3661 gaagccgccg ataccgagca tgatgagatc gccgcgctcg cgcgagctga tcggcaggtg
     3721 cgggagccgg ctcgcgcggg atctttcttc ctccagcgct tgcccggcga gcgccagcgg
     3781 cttcaagccg gcccggccgt gcggtgcgac cagatttcgc atcaaggcct cgcccagccc
     3841 cgcgcacgcg tcacggcttc gcgccagcgc gccattctgg attctctttc ggagccgggc
     3901 atccgcggct cgaagcgctt ctcggccttc caatgcgaag cgagctcctc gcgcgatctc
     3961 cagagattca cggtaagtcc tgcaaggtac gcggcgccga gcgccgtggt ctcgagcacg
     4021 cgcgggcgca cgaccggcac gccgagcagg tcggcctgga actgcatgag gaggtcgttc
     4081 gcggccgcgc cgccgtccac gcgcagctcc gcgagcggct gggccgagtc cttctgcatc
     4141 gcttccagga gctcggcgct ctggaacgcg atcgattcga gcgccgcgcg cgcgatgtgc
     4201 gctttggtgg tgccccgcgt taggccaacc atggcgccgc gcgcgtcggc atcccagtac
     4261 ggtgcgccca ggccagtgaa cgccgggact agatagacgt cgccggaatc gagcacgctc
     4321 gcggccagct tctccacctc gcgcgacgac gaaaaaaact ccaggccgtc gcgca
//
LOCUS       DASUNT010000231         4224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_644807, whole genome shotgun sequence.
ACCESSION   DASUNT010000231 DASUNT010000000
VERSION     DASUNT010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644807"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFX94_04605"
     CDS             complement(<1..301)
                     /locus_tag="VFX94_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5767498.1"
                     /translation="MRTPRHFLWEVQDRVGVITLNRPARKNPLTFDSYAELRDFFGEL
                     KNDKAIKAIVLTGAGGNFCSGGDVHEIIGPLTRMELAQLLEFTRMTGDLVKAMRRC"
     gene            complement(298..765)
                     /locus_tag="VFX94_04610"
     CDS             complement(298..765)
                     /locus_tag="VFX94_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5767499.1"
                     /translation="MDREFIARREDHSALRIWLRLLTCTQLIERRVRSGLREEFGTTL
                     PRFDLMAQLERHRGGLKMNELSRLLMVTGGNVTAIVDQLEKEGQVERLDEPDDRRAFR
                     IRLTRSGEKSFAEMARAHEQWIVELLGGLSRREQDELLRLLAKVKTHAVEASS"
     gene            complement(819..1388)
                     /locus_tag="VFX94_04615"
     CDS             complement(819..1388)
                     /locus_tag="VFX94_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5767500.1"
                     /translation="MSYHAGSRELQDRFDSRRLADRLAEKLWRTAFTTEDKTFIEASR
                     MFFLATADALGQPDCSYKGGLPGFVRVTGPSELSFPSYDGNGMFRSLGNVLVNPRVGM
                     LFLDFEQPRRLRVNGLATLLEDVAGFEGAQLVVRVAVEAIFPNCPRYIHRYGLVETSQ
                     YAPRPGYQPPEPNWKKNFSDVLPRSTKRD"
     gene            complement(1385..1777)
                     /locus_tag="VFX94_04620"
     CDS             complement(1385..1777)
                     /locus_tag="VFX94_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5767501.1"
                     /translation="MSKAALAGAEVTVILPVKELERARDFYVKQLGLDPEGLAPDGKF
                     VLRAKGTRVALMPKPGGTKAEHTALSFKVENIAADIKALKARGVSFHDYDLPGFKTVD
                     HIAVLANEKAAWFSDPEGNILCLHEEVK"
     gene            complement(1774..2133)
                     /locus_tag="VFX94_04625"
     CDS             complement(1774..2133)
                     /locus_tag="VFX94_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5767502.1"
                     /translation="MEAAERKDFRKPDEIREFPKGRLELIKIGGATVGRAVFEPGWRW
                     STSVQPLVHTKSCEAPHFQYHVSGVLRIRMDDGTEFDCKPGEVSLLPSGHDAWVVGNE
                     PAVVVDFQGMIDYARSR"
     gene            2203..2892
                     /locus_tag="VFX94_04630"
     CDS             2203..2892
                     /locus_tag="VFX94_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5767503.1"
                     /translation="MRTFKAALDLLGAMHEAARDKASFARLGVDGLPGLVASEFTTLS
                     ICHLASGKREVFGLPAGALSAEDRAAFDRHFHQHPLVRFHAYQGGRVPQRISDSVPFE
                     QFRRSALYNDYYRRIRIDHAIALPIYVRDGLLVSFVLNRTRRDFTDRERALLDLLKPQ
                     LAAIYRRINSVGQLTAREAEVLRWVGAGKSDAQIGAILRISARTVQKHLQNIYEKLGV
                     ESRTAAAMRVK"
     gene            complement(2885..3388)
                     /locus_tag="VFX94_04635"
     CDS             complement(2885..3388)
                     /locus_tag="VFX94_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767504.1"
                     /translation="MAPLANPPLTVAHRFVESEAGLAYYAKRPDGLMWRRKELFDTPV
                     YYLAFHGRPATVNPLLGRVDAEALCAAFANYGAYDNIAYFACCNVLRGEKGRRFARDF
                     LKASGMRAVIGYAATVDWMASLVADMLFLHRFYRDPSPWRSLRRIFESVRRDYPLARR
                     LGYTLIT"
     gene            complement(3475..>4224)
                     /locus_tag="VFX94_04640"
     CDS             complement(3475..>4224)
                     /locus_tag="VFX94_04640"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX5767505.1"
                     /translation="MSDLYAAGGIGAVLRELKPLLHLECMTVAGITLGERLDKEPTWV
                     DRAVVKPFGEPLSKEGGLVALFGSLAPNGAILKRSAADPKLFESEGRAVVFSSLDDLS
                     ERIDDPGLDVSADDFLVLQNAGPKSGYAMPEAGYLPIPAKLARAGVKDMVRISDARMS
                     GTAYGTIVLHVSPEAAVGGPLALVRNGDRMRLSVSERRIDLLVSEDEMKKRRSGWNAP
                     LTAPKRGYAKLYMDHVLQAEHGCDFDFLRKA"
BASE COUNT          706 a         1433 c         1358 g          727 t
ORIGIN      
        1 ggcagcggcg catcgccttc accaggtcgc ccgtcatgcg agtgaactcg aggagctgcg
       61 caagctccat ccgggtgagc ggcccgatga tctcgtgcac gtcgccgccc gagcagaagt
      121 tcccgccggc gccggtcagc acgatcgcct tgatcgcttt gtcgttcttc agctcgccga
      181 aaaaatcgcg cagctccgca tacgagtcga aagtgagcgg gttcttgcgc gcgggccggt
      241 tgagggtgat caccccgact ctgtcctgca cttcccacag gaaatgcctt ggcgtcctca
      301 tgagctcgcc tcgacggcgt gggtcttcac cttggcgagc agccgcagga gctcgtcctg
      361 ctcgcggcgc gaaagcccgc cgaggagctc gacgatccac tgctcgtgcg cgcgcgccat
      421 ctcggcgaag ctcttctcgc cgctgcgcgt aaggcggatg cggaacgcgc gccggtcgtc
      481 gggctcgtcc agccgctcga cctggccttc cttctcgagc tggtccacga tggcggtgac
      541 gttgcccccg gtgaccatga gcaggcgcga gagctcgttc atcttgagcc cgccgcggtg
      601 gcgctcgagc tgcgccatga gatcgaagcg cggcagcgtg gtgccgaact cctcgcgcag
      661 cccggagcgc acgcgccgct cgatgagctg ggtgcaggtg agcaggcgca gccagatgcg
      721 cagcgcgcta tggtcctcgc gccgggcgat gaattcgcgg tccatggcgg tactccaagc
      781 ctgaatagat caagcttgaa gtattcgcgg gccggttgtc aatctcgctt cgtgctgcgg
      841 ggcaaaacgt cgctgaagtt cttcttccag ttgggctccg ggggctggta ccccggccgc
      901 ggcgcgtact gcgaggtctc caccaggccg tagcgatgga tgtagcgcgg gcagttgggg
      961 aagatcgcct cgacggcgac gcgcaccacg agctgggcgc cctcgaagcc cgctacgtcc
     1021 tccagcaggg tcgccagccc gttgacccga agacgccgcg gctgctcgaa gtcgaggaag
     1081 agcatcccga cgcgcgggtt gacgagcacg ttgcccaggc tgcggaacat gccgttgccg
     1141 tcgtagctgg ggaacgagag ctccgaaggc ccggttacgc ggacgaagcc gggcagcccg
     1201 cccttgtagg agcagtcagg ctggccgagc gcgtccgcgg tggcgaggaa aaacatgcgg
     1261 ctcgcctcga tgaacgtttt gtcctcggtc gtgaacgcgg tgcgccacag cttctccgcc
     1321 agccggtcgg cgagccggcg gctgtcgaag cggtcctgca gctcgcgcga gccggcgtgg
     1381 tagctcactt cacttcttca tgcagacaga ggatgttgcc ttcgggatcg ctgaaccagg
     1441 ccgctttctc gtttgcgagc accgcgatgt ggtcgacagt cttgaacccc ggcagatcgt
     1501 aatcgtggaa gctgacgccg cgcgccttca gcgccttgat gtcggcggcg atgttttcga
     1561 ccttgaagct gagcgcggtg tgctcggcct tggtgccgcc cggcttgggc atcagcgcca
     1621 cccgcgtgcc tttggcgcgc aggacgaact tgccgtcggg cgccaggccc tcgggatcga
     1681 gcccaagctg cttcacgtag aaatcgcgcg cgcgctcgag ctccttcacc ggaaggatga
     1741 ccgtcacttc ggcgcccgca agcgcggctt tgctcatcgc gacctagcgt agtcgatcat
     1801 tccctggaaa tccactacca cggcgggctc gttgcccacg acccacgcat cgtggccgga
     1861 gggcagcagc gacacttcgc ccggcttgca gtcgaactcg gtgccgtcgt ccatccggat
     1921 cctgaggacc ccggagacgt ggtactgaaa atgcggtgct tcgcagctct tcgtatggac
     1981 gagcggctgc accgaagtgg accagcgcca cccgggctcg aacacggcgc gtcccacggt
     2041 cgcgcctccg atcttgatga gctccagacg gcccttgggg aattcgcgga tctcgtccgg
     2101 tttgcggaaa tccttcctct cggcggcttc catgacatgg ctcctgttgc gaatggcggc
     2161 ctatactttg gctccgcccc gccgcctgcg acatacgcaa atatgcgtac tttcaaggca
     2221 gccctagatc tcctcggcgc catgcatgag gcggcccggg acaaggcctc gttcgcccgg
     2281 ctcggagtgg atgggctccc ggggctggtc gcctcggagt tcaccacgct ttcgatctgc
     2341 cacctggcaa gcggcaagcg ggaagtgttc ggcctgccgg ccggagcgct gagcgccgag
     2401 gaccgcgccg ccttcgaccg gcatttccac cagcatccgc tggtgcgttt ccacgcctac
     2461 cagggcggcc gcgtcccgca gcgcatcagc gactcggtgc ccttcgagca gttccgccgc
     2521 agcgcgctct acaacgacta ttaccgccgc atccgcatcg accacgcgat cgcgctgccg
     2581 atttacgtgc gcgacggcct gctcgtcagc ttcgtcctga accgcacgcg gcgcgacttc
     2641 accgaccgtg agcgggcgct gctcgacctg cttaagccgc aactcgccgc catctaccgg
     2701 cgcatcaaca gcgtcggaca actcacggcg cgtgaagccg aagtgcttcg ctgggtgggc
     2761 gccgggaaaa gcgacgcgca gatcggcgcg atcctgcgca tcagcgcgcg caccgtgcag
     2821 aagcacctgc agaacatcta cgaaaagctc ggcgtcgaga gccgcacggc tgcggcgatg
     2881 cgggtcaagt aatgagggtg taacccaggc gtcgggcgag cggatagtcc cgccgcaccg
     2941 attcgaagat gcgccgcagg cttcgccacg gcgacggatc ccggtagaac cggtgcagaa
     3001 acagcatgtc ggccaccagg ctcgccatcc agtccactgt cgcggcgtag ccgatcaccg
     3061 ccctcatgcc cgacgccttg agaaagtcgc gcgcgaagcg ccggcccttc tctccgcgca
     3121 gcacgttaca gcaggcaaag taagcgatgt tgtcgtacgc gccgtaattg gcgaacgccg
     3181 cgcacagcgc ctcggcgtcg acgcggccga gaagcgggtt caccgtcgcc ggcctgccat
     3241 ggaaagcaag gtagtacacc ggcgtatcga agagctcctt gcggcgccac atcaggccgt
     3301 cgggccgctt cgcatagtag gcaagccccg cctcggactc gacgaagcgg tgcgcaaccg
     3361 tgagcggcgg attggcgagc ggcgccattg cctcgaagaa ccccttgacc gagaagctct
     3421 ggaacagctt ctcgttccag taggcctcga ggcagacgag cttcggcggc gccctcaggc
     3481 ttttctcaag aaatcgaaat cgcagccgtg ctcggcctgc agcacgtggt ccatgtagag
     3541 cttcgcgtag ccgcgctttg gtgccgtgag cggggcgttc cagccgcttc ttcttttttt
     3601 catttcatct tcggacacga gcaagtctat ccgcctttca ctcacggaaa gcctcatccg
     3661 gtcaccgttc cgaacgagcg cgagcgggcc gccgacggcg gcctcgggcg aaacgtgcag
     3721 cacgatggtg ccgtacgccg tgccgctcat gcgcgcgtcc gagatgcgca ccatgtcctt
     3781 cacgccggcg cgcgccagct tcgcgggaat cggcaggtag cccgcctcgg gcatcgcata
     3841 gcccgacttc gggccggcgt tctgcagcac gaggaaatcg tcggcgctta cgtcgagccc
     3901 ggggtcgtca atgcgctcgg aaaggtcatc gagtgagctg aacaccaccg cccgcccttc
     3961 gctctcgaaa agtttcggat ccgccgccga gcgcttcagg atcgcgccgt tgggcgcgag
     4021 cgagccgaac agcgcgacca ggccgccttc cttagatagc ggctcgccaa acggcttgac
     4081 caccgcccga tccacccaag tcggctcctt gtcgagccgc tcgccgaggg tgatgcccgc
     4141 gacggtcatg cattcgaggt gcagcagcgg cttcagctca cggagaacag cgccgatccc
     4201 gccggccgca tacaggtccg acat
//
LOCUS       DASUNT010000232         4697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_732341, whole genome shotgun sequence.
ACCESSION   DASUNT010000232 DASUNT010000000
VERSION     DASUNT010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732341"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /gene="tsf"
                     /locus_tag="VFX94_04645"
     CDS             <1..470
                     /gene="tsf"
                     /locus_tag="VFX94_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX5767506.1"
                     /translation="RQALIQKIGENITIRRFKRMQAKGKLALYIHAGSKIGVLVDYEG
                     PEETGKDLAMHIAFGKPAYMSKTDVPHETVQEERRIQEARAKESGKPPAIIAQIVEGA
                     VNKALGEKTLLGQPFVRDDKQTVEKMLAGKKAKVHGYAFVIVGEGIDKPQQVH"
     gene            467..1180
                     /gene="pyrH"
                     /locus_tag="VFX94_04650"
     CDS             467..1180
                     /gene="pyrH"
                     /locus_tag="VFX94_04650"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX5767507.1"
                     /translation="MTPKYKRVLLKLSGEALMGDDPFGINRQTIETIVGEVTQVSRLG
                     VELGVVIGGGNIFRGIAPGAAGMDRATADYMGMLATVMNALALSDAMRQAGLASRVQS
                     ALRIEQVVEPYIRGKAIRYLEEGKVVIFAAGTGNPFFTTDTAAALRGSEMGAEIVLKG
                     TKVDGVYTADPKRDPRAERYAKISFDEAIARNLRVMDATALTLCRDQKLPIAVFSIFK
                     RGALRRVVLGEDEGTLVHV"
     gene            1203..1763
                     /gene="frr"
                     /locus_tag="VFX94_04655"
     CDS             1203..1763
                     /gene="frr"
                     /locus_tag="VFX94_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX5767508.1"
                     /translation="MVIAELKKTTEQKMDKSVQAFKADLGKIRTGRAHTGLLDHIHVD
                     YYGTPTPLNQVAKVVLIDARTIGVTPFEKKMAQAIEKAIRDSDLGLNPASSGETVRVP
                     MPPLTEERRKELIKVVKHEAENARVAVRNLRRDAIHQLKEALKKHEVSENDERRALDE
                     MQKMTDKHVAAIDRLLQEKEKELLAV"
     gene            1760..2539
                     /gene="uppS"
                     /locus_tag="VFX94_04660"
     CDS             1760..2539
                     /gene="uppS"
                     /locus_tag="VFX94_04660"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX5767509.1"
                     /translation="MTPMPLEPSSTRAIPQAGELPRHVAIIMDGNGRWAQRRRLPRIA
                     GHRRGVEAVRSTVRACAERGIEFLTLFAFSSENWRRPAEEVALLMKLFKSALESEVEK
                     LHANAVRVKVVGDTSRFDPAIRALIERADALTAENRRLTLTVAANYGGRWDIMQAMSR
                     LLEDRKLAPGAVDEALFSPYLAMSYAPEPDLFIRTGGEQRISNFLLWQLAYTELYFTE
                     LLWPDFGPAALDEAIASYRRRERRFGRTSEQVQQSKRADVA"
     gene            2544..3314
                     /locus_tag="VFX94_04665"
     CDS             2544..3314
                     /locus_tag="VFX94_04665"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX5767510.1"
                     /translation="MRRAGTAVVLLAALVAALIVLPRAGILVLMALVAGAAAFEWARL
                     CAVHAPLYAAAVVLALLLAFWAGVSQGMFLLAAAFWLLAAPAWLWVRVGPEHRAALAA
                     AGFVVLVPAALAMVLLEPGEALLVLAVAWIADTGAYFAGTRWGRRKLAPAISPGKTWE
                     GAAGGLIGAAAYAMILSLILAGAQATRMAAFVGAAALLVTVSIVGDLFESAAKRQAGV
                     KDSGALLPGHGGVLDRIDSATAVLPVAALVVPFLKGPA"
     gene            3311..4462
                     /locus_tag="VFX94_04670"
     CDS             3311..4462
                     /locus_tag="VFX94_04670"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX5767511.1"
                     /translation="MNLAIFGATGSIGASTLDVAARHPERYRLFALTANGSAAQLAQL
                     CARHRPRFAVLSGLADDRELRRSFAAFGTELLFGVDALQGLARHPEVDAVMAAIVGAA
                     GLASTLAAVRAGKRVLLANKEALVMAGPLVQRAARQSGAQIVPVDSEHNAVFQCFSGS
                     KFVRKIVLTASGGPFRGAAPESLAAVTPEQACAHPNWTMGRKISVDSATMMNKGLEVI
                     EARWLFDLPPERIEVLIHPQSIVHSLVEYADGSMIAQLSNPDMRVPIANALAHPERVE
                     SGAAALDLAAIRTLSFEKPDPARFPCLGLAYSALRDGGAAPAVLNAANEVAVDAFLAG
                     RLAFTGISGVIADTLCALPTGPAEELAQVMEADARAREFAGTRILARAA"
     gene            4472..>4697
                     /locus_tag="VFX94_04675"
     CDS             4472..>4697
                     /locus_tag="VFX94_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5767512.1"
                     /translation="MTLLHTIIAFIVALGLLIVVHELGHYIVARWCGVKVLRFSVGFG
                     RALGRWQRGETEWVVAAIPFGGYVKMLDERE"
BASE COUNT          753 a         1568 c         1638 g          738 t
ORIGIN      
        1 ggcgccaggc gctcatccag aagatcggcg agaacatcac catccgccga ttcaagcgca
       61 tgcaggcaaa gggaaagctt gcgctctaca tccacgccgg aagcaagatc ggcgtcctgg
      121 tcgactacga gggccctgaa gagaccggca aggacttggc gatgcacatc gcgttcggca
      181 agccggcgta catgagcaag acggacgttc cgcacgaaac ggtccaggag gagcgccgca
      241 tccaggaagc ccgggccaag gaatcgggca agccgcccgc gatcattgcg cagatcgtcg
      301 agggcgcggt caacaaggcg ctcggcgaga agacgctgct gggacagccg tttgtgcgcg
      361 acgacaagca gacggtggag aagatgctcg ccgggaagaa ggcgaaggtg cacggctacg
      421 ctttcgtcat cgtaggcgag ggcatcgaca agccccagca agtgcattga cacccaaata
      481 caaacgcgtg cttctcaagc tgtcgggcga agccctgatg ggcgatgatc cgttcggcat
      541 caaccggcag acgatcgaga ccatcgtggg cgaggtgacg caggtgtcgc gcctcggcgt
      601 ggagctcggc gtggtgatcg gcgggggaaa tatcttccgc ggcatcgcac ccggcgcggc
      661 cggcatggac cgggcgaccg ccgactacat gggaatgctc gccacggtga tgaacgcgct
      721 cgcgttgtcc gatgcaatgc gccaggcggg gctcgcgagc cgcgtgcagt cggcgctcag
      781 aatcgagcag gtggtggagc cttacatccg cggcaaggcc atccgctatc tggaagaggg
      841 caaagtggta atttttgccg ctggcaccgg gaatcctttc ttcaccaccg ataccgccgc
      901 ggcgctgcgc ggcagcgaga tgggcgcgga gatcgtgcta aagggcacga aggtcgacgg
      961 cgtgtacacg gccgacccga agcgcgatcc acgtgccgag cgctacgcga agatcagctt
     1021 cgacgaggcg atcgcgcgca acctgcgtgt gatggatgcc acagcgctca ccctgtgccg
     1081 cgaccagaag ctgccgatcg ccgtcttcag catcttcaag cgcggcgcgc tgcgccgcgt
     1141 ggtgcttggc gaggacgagg gcacgctggt tcacgtatga tggtggcgag gtcgctatgg
     1201 ctatggtgat tgcggagctg aagaagacga cggaacagaa gatggacaag agtgtccagg
     1261 cgttcaaggc tgatctgggc aagattcgca ccggccgcgc gcacacgggg ctgctcgatc
     1321 acatccacgt ggactactac ggcacgccga cgccgctcaa ccaggtggcg aaggtggtgc
     1381 tgatcgacgc gcgcacgatc ggggtcacgc cgttcgagaa gaagatggcg caggcgatcg
     1441 agaaggcgat ccgcgattcc gacctcggcc taaatccggc ttccagcggc gagacggtgc
     1501 gcgtgcccat gccgccgctc accgaggagc ggcgcaagga gctgatcaag gtggtgaagc
     1561 acgaggcgga gaacgcgcgc gtcgcggtgc gcaacctgcg ccgcgatgcg atccaccagc
     1621 tcaaggaggc gctgaagaag cacgaggtgt ccgaaaacga cgagcgccgt gcgctcgacg
     1681 agatgcagaa aatgaccgac aagcatgtcg ccgcgatcga ccgcctgctg caggagaagg
     1741 agaaggagct gctcgcggta tgacccccat gccgctcgag ccgagctcca cgcgcgcgat
     1801 cccgcaggcg ggggagctgc cgcgccatgt cgccattatc atggacggca acggccgctg
     1861 ggcccagcgc cggcgcctgc cgcgcatcgc ggggcaccgg cgcggcgtcg aggcggtgcg
     1921 ctcgacggtg cgcgcctgcg ccgagcgcgg catcgaattt ctcacgctgt tcgccttcag
     1981 ctccgagaac tggcgccgcc ccgccgaaga ggtggcgctg ctcatgaagc tgttcaagag
     2041 cgcgctcgag agcgaggtcg agaaactcca cgcgaacgcg gtgcgcgtca aggtggtggg
     2101 cgacacctcg cgcttcgacc cggcgatcag ggcgctgatc gagcgcgccg atgcgctgac
     2161 cgccgagaac cggcgcctca cgctgacggt cgccgcaaac tacggcggcc gctgggacat
     2221 catgcaggcg atgtcccggc tgctggagga tagaaagctc gcgccgggcg cggtggacga
     2281 ggcgctcttt tcgccctacc tcgcgatgag ttacgcgccg gagcctgatc tcttcatccg
     2341 caccgggggc gagcagcgca tcagcaactt tctgctctgg cagctcgcct acaccgagct
     2401 ctattttacc gaactgctgt ggccggactt cggtcccgcc gcgctcgacg aggcgatcgc
     2461 ctcctaccgc aggcgcgagc gccgcttcgg gcgcacgagc gagcaggttc agcagtcgaa
     2521 gcgcgccgac gttgcgtgac tttttgaggc gggccggcac tgccgtcgtg ctgctggcgg
     2581 cgctcgtcgc ggcgctcatc gtgctgccgc gcgccggaat cctggtgctg atggcgctcg
     2641 tcgcgggcgc cgctgccttc gaatgggcgc gcctgtgcgc cgtgcacgcg cctctctacg
     2701 ccgccgccgt cgtgcttgcg ctgctgctcg cgttctgggc cggcgtgtca caggggatgt
     2761 tcctgctcgc cgcggcattc tggctcctcg ccgcgccggc ttggctctgg gtgcgcgtgg
     2821 ggcctgagca ccgcgccgcg ctcgccgccg ccgggtttgt ggtcctcgtg cccgccgcgc
     2881 tcgcgatggt gctgctcgag cccggcgaag cgctcctcgt cctcgcggtg gcctggatcg
     2941 cggacaccgg cgcctatttc gccggcacgc gctggggacg gcgcaagctc gccccggcga
     3001 tcagcccggg caagacctgg gagggcgccg ccggcggcct catcggcgct gctgcctacg
     3061 ctatgattct ttcgcttatt ctcgcaggag cccaggcaac ccggatggcg gcttttgtcg
     3121 gcgcggcagc gctgctcgtg acggtcagca tcgtcggcga cctgttcgaa tccgcggcca
     3181 agcggcaggc cggagtgaag gacagcggcg cgctgctgcc cggccacggc ggcgtgctcg
     3241 accgcatcga cagcgcgacc gcggtcctgc cggtcgcggc gctcgtcgtg ccgtttctga
     3301 agggccccgc gtgaacctcg ccattttcgg cgccaccgga tcgatcggcg cgagcacgct
     3361 cgacgtggcc gcacgccacc cggagcgcta tcggctgttc gccctgaccg cgaacggctc
     3421 cgcggcgcag ctcgcgcagc tttgcgcgcg tcaccggccg cgcttcgccg tgctgtcggg
     3481 cctcgccgac gatcgggagc ttcgccgcag cttcgccgcc ttcggcaccg agctcctctt
     3541 cggcgtcgat gcgctgcaag gtcttgcgcg gcatccggaa gtcgacgccg tcatggcagc
     3601 catcgtcggc gcggcgggac tggcctccac gcttgccgcg gtgcgcgccg gcaagcgcgt
     3661 gctgctcgcc aacaaggagg cgctggtcat ggcggggccg ctcgtgcagc gcgccgcgcg
     3721 ccagtcgggc gcgcagatcg tgcccgtcga cagcgagcac aacgccgtgt tccagtgctt
     3781 ttcgggcagc aagttcgtga ggaagatcgt gcttaccgct tcgggagggc cgtttcgcgg
     3841 cgccgcgccg gaaagcctcg ctgccgtgac gcccgagcag gcgtgcgcgc atccgaactg
     3901 gacgatgggc cgcaagattt cggtcgactc cgcgacgatg atgaacaagg gcctggaggt
     3961 catcgaggcg cgctggctgt tcgacctgcc gcccgagcgc atcgaggtgc tgatccatcc
     4021 gcaaagcatc gtgcactcgc tggtcgagta cgccgacggc tcgatgatcg cgcagctctc
     4081 caatcccgac atgcgcgttc cgatcgccaa cgcgctcgca caccccgagc gcgtggagtc
     4141 cggagccgcg gcgctcgacc ttgccgcgat acgaactctg tctttcgaga aacccgatcc
     4201 ggcgcgcttt ccgtgcctcg gcctcgccta ttccgcgctg cgggacggcg gcgcggcgcc
     4261 cgccgtgctg aacgccgcca acgaggtggc ggtcgacgcg ttcctcgccg gccgcctcgc
     4321 gttcaccggc atcagcggcg tgatcgccga cacgctttgc gccctgccca ccgggcctgc
     4381 cgaagagctg gcgcaggtca tggaagccga tgcgcgcgcg cgcgagtttg cgggcacgag
     4441 gatcctggcg cgcgccgcct gaccacgccc catgaccttg ctgcatacca tcatcgcctt
     4501 catcgtcgcg ctcgggctgc ttatcgtcgt gcacgagctc ggccactaca tcgtcgctcg
     4561 ctggtgcggc gtcaaggtgc tgcgcttttc cgtcggcttc gggcgggcgc tcgggaggtg
     4621 gcagcgcggg gagacggagt gggtcgtcgc cgcgattccg ttcggcggct acgtcaagat
     4681 gctcgacgag cgcgaag
//
LOCUS       DASUNT010000233         2898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1259653, whole genome shotgun sequence.
ACCESSION   DASUNT010000233 DASUNT010000000
VERSION     DASUNT010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259653"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VFX94_04680"
     CDS             <1..745
                     /locus_tag="VFX94_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767513.1"
                     /translation="IRTNERAIEADETYIRSQNPALGAYVAGYYPHNYDFLAFAAAMI
                     RREEQAISAAEKMAALAIPEMLRAPGMTFTQHHLTRHLQMKVRFARWQDILRAEPPAE
                     DLPHARAMWSYARGRAQAAQGATAQAEADLAHVRAARDDKALASQRLEFNTSGQILAI
                     AAEVLAGHIAQAKGEHAAAIAHLRKAAQLEDELTYGEPPEWSVPVRQELGLALIRAAR
                     PAEAAQAFREDLKRFPENSWSLAGLEQAH"
     gene            complement(793..1932)
                     /locus_tag="VFX94_04685"
     CDS             complement(793..1932)
                     /locus_tag="VFX94_04685"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5767514.1"
                     /translation="MNPLFFGGALGALAMYFLDPNLGRRRRARTRDRVTHAVKVVNDG
                     ARVTARDTVHRAHGAWAEARRLFAHEGEVGDEALVARVRAALGRVVSHPHALAVEASG
                     GHVTLKGPILAYEVRPLLRIVRRVLGVRAVSDQLTVHKEAGNVSSLQGGEPRSGERFE
                     LLQENWSPAARVIAGGAGAALMLAATRARGGLCALLGVSGSALLARATTNRDLPSLLG
                     FGERGITVQKTIHVAAPVEQVFAFWTDYQNFPRFMHNVREVRPVSENRSHWVVAGPAG
                     VPVQWTAEVTRVVPGALIEWCSVRDSDVRHQGAVSFAENGDGGTRVSVELSYIPPAGA
                     FGHAVAAIFGADPKSEMDADLLRMKSMIETGHPPHDAAKPQPRES"
     gene            1966..2436
                     /locus_tag="VFX94_04690"
     CDS             1966..2436
                     /locus_tag="VFX94_04690"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEX5767515.1"
                     /translation="MATTWIVAADESRARILQVMDPERHLNEVEDLVNPAGRAQDREL
                     QTDAEPRFDGRVRKPGTARTGGPASDSERQGAVEHSVRTFAREVGRYLGKARIAQRFD
                     RLVLVAPPKFLGALRKELHKEVEKLVAEELPKDLSWLNAREIERYFSKGSARAP"
     gene            complement(2381..>2898)
                     /locus_tag="VFX94_04695"
     CDS             complement(2381..>2898)
                     /locus_tag="VFX94_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5767516.1"
                     /translation="ERLQLCAACHGPDGNSTDPQIPSIAGQPKLFLENQLILFREELR
                     KSDQMLPVVKGLKDPEIVKLAEHFSKLPAKGMESGVADPKSMKLGAERAKALRCGVCH
                     LADYSGQNQIPRLSGQREAYLEAEMRAYRDGKRTGGDTIMAAALYGVGDADIKALAHY
                     LSRSSARSPAR"
BASE COUNT          471 a         1023 c          974 g          430 t
ORIGIN      
        1 catccggacc aacgagcgcg cgatcgaggc cgacgagacg tacatccgca gccagaaccc
       61 cgcgctcggg gcgtacgtcg ccggctacta cccccacaat tacgacttcc tcgccttcgc
      121 cgccgccatg atccggcgcg aggagcaagc gatatcggcc gccgagaaga tggccgcgct
      181 cgccatcccc gagatgctgc gcgcgccggg catgacgttt acccagcacc atctcacccg
      241 gcaccttcag atgaaagtgc gcttcgcgcg ctggcaggac atcctgcgcg ccgagccgcc
      301 cgcggaggat ttaccgcacg cgcgggcgat gtggagctac gcgcggggcc gcgcgcaggc
      361 cgcgcagggc gcgactgcgc aggccgaggc ggacctcgcc cacgtgcgcg ccgcgcgcga
      421 cgacaaggcg ctcgcctcgc aacgcctcga gttcaacact tccggacaga tccttgccat
      481 cgccgccgaa gttctcgcgg gccacattgc gcaggcgaaa ggcgagcatg ctgcagcgat
      541 agcgcacctg cgcaaggcgg cgcagctcga ggacgagttg acgtacggtg agccgcccga
      601 gtggagcgtg cccgtgcgcc aggagctcgg ccttgcgctc atccgggccg cgcgtcccgc
      661 cgaagcggcc caggccttcc gcgaggatct caagcgcttc cccgagaact cctggtcgct
      721 cgccggcctc gagcaggccc actaaaaaag ggtcaggacc cttttattgg aaagggggtc
      781 ctgacccctt tttcagcttt cgcgcggctg cggcttcgcc gcgtcgtgcg gcgggtggcc
      841 ggtttcgatc atcgacttca tgcgcagcag gtcggcgtcc atctcgcttt tcgggtcggc
      901 gccgaagatc gccgccacgg cgtggccgaa ggcgccggcc ggcggaatat aggagagctc
      961 gacgctcact cgcgtgccgc cgtcgccgtt ctcggcgaag gacacggcgc cctggtggcg
     1021 cacgtcggag tcgcggaccg agcaccactc gatcagcgcg ccgggcacca cgcgggtcac
     1081 ctccgcggtc cactgcaccg gcacgccggc cgggccggcg accacccagt gcgagcggtt
     1141 ctcggagacc gggcgcacct cgcgcacgtt gtgcatgaac cgcgggaagt tctggtaatc
     1201 ggtccagaac gcaaatacct gctcgaccgg cgccgcaacg tggatcgtct tctgcaccgt
     1261 gatgccgcgc tcgccgaagc cgagcaggct ggggaggtcg cggttggtgg tcgcgcgcgc
     1321 aagcaaagca ctacccgata ctccaagcag cgcgcagagc ccgccgcgcg cgcgcgtcgc
     1381 cgcgagcatc agcgcggcgc cagcaccgcc cgcgatcacc cgcgccgccg gagaccagtt
     1441 ctcctgcaga agctcgaagc gctcgcccga gcgcggctcg ccgccctgga gcgaagagac
     1501 attgcccgcc tccttgtgca ccgtgagctg atcgctcacc gcccgcacgc cgaggacgcg
     1561 gcgcacgatg cgcagcagcg ggcgcacctc gtaggcgaga atcgggccct tcagcgtaac
     1621 atggccgccg ctcgcttcga cagcgagcgc gtggggatgc gataccaccc ggcccagcgc
     1681 ggcgcgcacg cgcgcgacaa gcgcctcatc gccgacctcg ccttcgtgcg caaagagccg
     1741 cctcgcctcc gcccaggcgc cgtgcgcgcg gtgcaccgtg tcgcgcgcgg ttacgcgcgc
     1801 gccgtcgttc acgaccttga cggcgtgggt cacccggtcg cgtgtgcgcg cgcggcgccg
     1861 ccgccccaga ttcggatcga gaaaatacat cgcgagcgcg cccagggcgc cgccaaaaaa
     1921 tagagggttc atggcatttt caggttagtc ggcagtcgaa tgttcatggc tactacgtgg
     1981 atcgtcgcgg cagacgaaag ccgtgccagg attctgcaag ttatggaccc ggagcggcac
     2041 ctgaacgagg tggaggacct cgtcaacccc gccggccgcg cccaggaccg cgagctgcaa
     2101 accgatgccg agccgcgctt cgacggccga gtgcgcaagc cgggcacggc gcgcaccggc
     2161 ggcccggcga gcgacagcga gcggcaaggc gcggtcgagc acagcgtgcg taccttcgcg
     2221 cgcgaggtcg gccgctacct cggcaaggcg cgcatcgcgc agcgctttga ccggctggtg
     2281 ctggttgcgc cgccgaaatt cctcggcgcg ctgcgcaagg agctgcacaa ggaggtcgag
     2341 aagctcgtcg ccgaggagct gccgaaagac ctctcgtggc tcaacgcgcg ggagatcgag
     2401 cgctacttct cgaaaggtag tgcgcgagcg ccttgatgtc ggcgtcgccc accccgtaga
     2461 gcgcggccgc catgatggtg tcgccgccgg tgcgcttgcc gtcgcgatag gcgcgcatct
     2521 cggcttcgag atatgcctcg cgctggccgg agagacgcgg gatctggttt tggccgctgt
     2581 agtccgcgag atgacacacc ccgcagcgca gggctttcgc cctttctgct ccaagcttca
     2641 tcgatttcgg gtccgcgacg ccgctttcca tgcccttagc cggcagtttg gagaaatgct
     2701 cggcgagctt cacgatctcg ggatccttca gccccttcac caccggaagc atctggtctg
     2761 acttgcgcag ctcctcgcga aagaggatca gctggttctc gaggaagagc ttcggctgcc
     2821 ccgcgatcga ggggatctgc ggatcggtgg agttgccgtc cgggccgtgg cacgcggcgc
     2881 acagctggag cctttctt
//
LOCUS       DASUNT010000234         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_382824, whole genome shotgun sequence.
ACCESSION   DASUNT010000234 DASUNT010000000
VERSION     DASUNT010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382824"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1085
                     /gene="queG"
                     /locus_tag="VFX94_04700"
     CDS             33..1085
                     /gene="queG"
                     /locus_tag="VFX94_04700"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX5767517.1"
                     /translation="MAATSGNLISSIKEWGREIGFQAVGIADADLSAAEPRLLEWLGR
                     GWHGEMEYMARHGALRARPAELKPGTLRVISCRMDYLNDVAKGISIDGEKANIARYAR
                     GRDYHKVLRERLRKLCERIAADIGEFGCRVFTDSAPVMEVELAARAGIGWRGKHTLLL
                     SRDAGSWFFLGEIYCDLPLETDSEVADSCGTCERCIEVCPTQAIRGPYQLDARRCISY
                     LTIEHKSAIPEELRALIGNRVYGCDDCQLVCPWNRFAQLAKETDFQSRNGLDKATLVE
                     LFGWTQAQFDERLRGSPIRRIGYERWLRNLAVGLGNAPSSPQVLAALEGKKDHPSALV
                     REHVRWALSRHASLAA"
     gene            1151..1963
                     /locus_tag="VFX94_04705"
     CDS             1151..1963
                     /locus_tag="VFX94_04705"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5767518.1"
                     /translation="MRRWCACRLQPRPGGAVLWVHGFVDYFFQRHVAERFAMEGYAFY
                     ALDLRKHGRSLRLHQHPNFCKSLAEYYPDITSSIDVIGEPVLLAGHSTGGLITALYAH
                     EGPHRARVRGLWLNSPFFDWKLPDWRKAQIHMAAAFGRFFPVLNDAKALLRDYGEALV
                     DYGWEFDTALKPIEGFPVYYGWLGAITDAHHCVHRGLDIFCPVLAMHSDEADIVLDWR
                     HIARWSRGLGREVTVLAFPGAQHDVTLSRPEIREEMFRQLFAWAERAVMSPA"
     gene            complement(1914..2279)
                     /locus_tag="VFX94_04710"
     CDS             complement(1914..2279)
                     /locus_tag="VFX94_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEX5767519.1"
                     /translation="MADAILVVHFLIVLFIVGGLLLVWAGAALGWRWVRNPWFRYLHL
                     GAIGFVAAEALLGVVCPLTVWEDMLRGGARAESFVGRWVRYFLYYEAPEWVFTALYVA
                     WAAATLATLRLVPPTRRAA"
BASE COUNT          414 a          790 c          780 g          376 t
ORIGIN      
        1 ccccgccggc cagcgcctcg cccaggcgta acgtggccgc cacgtcgggc aacctaatct
       61 cgagcatcaa agaatggggc cgcgagatcg gcttccaggc ggtagggatc gcggatgccg
      121 acctgtccgc cgccgagccg cggctccttg agtggctggg acggggctgg catggcgaga
      181 tggagtatat ggcgcgccat ggggcgctcc gcgcgcgtcc ggccgagctc aagccgggca
      241 cgctgcgcgt catctcgtgc cgcatggact acctgaacga cgttgccaag ggaatctcca
      301 tcgacggcga aaaggcaaac atcgcgcgct acgccagagg ccgcgactat cacaaggtgc
      361 tgcgcgagcg gctgcgcaag ctctgcgagc gcatcgccgc cgacatcggc gagttcggct
      421 gtcgggtatt caccgattcg gcgccggtga tggaagtcga gctcgccgcg cgcgcgggca
      481 tcggctggcg cggaaagcat acgctgctcc tcagccgcga cgccggctcg tggttcttcc
      541 tgggagagat ctattgcgat ctgccgctcg aaaccgacag cgaagttgcc gattcctgcg
      601 gcacttgcga gcgctgcatc gaggtctgcc cgacccaggc gatacgaggg ccctatcagc
      661 tcgatgcccg ccgctgcatt tcgtacctca ccatcgagca caagagcgcc attcccgagg
      721 agctgcgtgc gctcatcggc aaccgcgttt acggctgcga cgactgtcag ctggtgtgtc
      781 cgtggaacag atttgcccaa ctagcgaaag aaacggattt tcaatcgcgc aatggcctgg
      841 acaaggccac gctggtcgag ctgttcggct ggacgcaagc ccagttcgac gagcggctgc
      901 gcggctcgcc catccggcgc atcggctacg agcgctggct gcgcaacctc gcggtgggcc
      961 tgggaaacgc gcccagctcg ccgcaggtat tagcggccct ggaaggaaag aaagaccatc
     1021 cgtccgcgct agtcagggag cacgtgcgtt gggcactctc gcgccatgca agcctggcag
     1081 cctgatcgcc tgctccgcgg cttcgaagcg ctcgagctcg attttccgga cgactacgac
     1141 ggccgcgtgc ttgcgacgct ggtgcgcctg ccggctgcag ccgcgccccg ggggggcggt
     1201 gctgtgggtg cacggcttcg tcgattattt tttccagcgg catgtggccg agcgcttcgc
     1261 gatggaaggc tatgccttct acgcgctcga cttgcgaaag catggccgct cgctgcggct
     1321 ccaccagcac ccgaacttct gcaagagcct cgccgagtac tacccggaca tcacgagctc
     1381 gatcgacgtg atcggcgagc cggtgctgct cgccggacat tccaccggcg ggcttatcac
     1441 cgcgctctat gcccacgaag gaccgcatcg cgcgcgcgtg cgggggctgt ggctgaacag
     1501 tccgttcttc gactggaagc tgccggactg gcgcaaggcg cagatccaca tggcggcggc
     1561 cttcggccgc ttctttcctg tcctcaacga cgccaaggcg ctgctacgcg actacggcga
     1621 ggcgctggtg gactatggat gggaattcga caccgcgctc aagccgatcg aaggcttccc
     1681 ggtgtactac ggctggctcg gcgccattac cgacgcgcat cactgcgtgc accgcggcct
     1741 ggacatcttc tgcccggtgc tcgccatgca ctcggacgag gccgacatcg tgctcgactg
     1801 gcgccacatc gcgcgctggt cgcgcgggct cggccgcgag gtgacggtgc tcgcgtttcc
     1861 cggcgcgcag cacgatgtca ccctgtcgcg gccggaaatc cgcgaggaaa tgttcaggca
     1921 gctcttcgcg tgggcggaac gagccgtaat gtcgccagcg tagcggcggc ccaggcgacg
     1981 tagagagccg tgaagaccca ttcgggcgcc tcgtagtaga ggaagtagcg cacccagcga
     2041 cccacgaacg attcggcgcg cgcgccgccc cgcagcatgt cttcccatac ggttagcggg
     2101 cacaccacgc cgaggagcgc ctcggcggcg acaaatccga tcgcgcccaa gtgcaggtag
     2161 cggaaccacg ggttacgcac ccagcgccag ccgagcgcgg cgccggccca gacgagcagg
     2221 agcccgccga cgatgaagag cacgatcagg aagtggacga cgaggatggc gtccgccatg
     2281 cttctagcat ggcacaacgc ttccccaggc tcacccacga gcaaatgacc ccggcccaga
     2341 gagaggtcgc ggccgagatc
//
LOCUS       DASUNT010000235         2367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_207941, whole genome shotgun sequence.
ACCESSION   DASUNT010000235 DASUNT010000000
VERSION     DASUNT010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207941"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1764
                     /locus_tag="VFX94_04715"
     CDS             <1..1764
                     /locus_tag="VFX94_04715"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX5767520.1"
                     /translation="TVRPVDGIDFELRRGECFALVGESGSGKSMTALSLMRLLPEAGR
                     IASGRVALDGIDLLALPEAAMRAVRGRRVAMVFQEPSTALNPVLTVGRQIVEVLERHT
                     NKTPTVNRQTAIELLRAVGIRDAERRFDEYPFQLSGGLKQRAMIAAALAVDPEVLIAD
                     EPTTALDVTIQAQILELLARLQAERGMTLLLITHDLGIVARMAHRIAVMYAGEIVEIA
                     GRDAFLRAPQHPYSQKLFAALPTPQRRARELAVIRGQVPALTKKFDHCRFAERCDYVF
                     ERCRVEAPRLLEPSPGHFARCHLREQGPASPRMVGDAREEGTVRQEAGKGVLLEAKDL
                     KVYFPIRKGLFKRTVGYVRAVDGVSLELREGRTLALVGESGCGKTTTGKAILQLIPPS
                     GGTVRFAGEALTGLSRSQLRPWRGAMQIVFQDPYGSLNPRLRVAEILLEGMRSLGVGS
                     SDAQRTERLKQLLAEVGLPPDSLERYPHECSGGQRQRIAIARALAVDPRLIVCDEPTS
                     ALDVSVQAQILNLLRALQERLGIAYLFITHNIAVVDYLAHDVAVMHQGGIVERGTVDA
                     VLRRPQHPYTKQLLAAVPAID"
     gene            1764..>2367
                     /locus_tag="VFX94_04720"
     CDS             1764..>2367
                     /locus_tag="VFX94_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5767521.1"
                     /translation="MRICWLIALALVSPALHAAHAYSQFGDVKYPPGFRHFEWVNPDA
                     PKGGAIELVPPLRITNFDKYNPFTLKGTAPPGTGLLFESLLTGTMDEPTTAYGLLAED
                     VEVAPDGLSASFRINPAARFHDGTPVTAADAKHSFDMLMSKQAAPQYRVIYGDIKQAV
                     VLGPRMIRYDFKRPNAELPLMAGGLPVFSRAWGAGKPFDQV"
BASE COUNT          376 a          827 c          793 g          371 t
ORIGIN      
        1 accgtgcgcc cggtcgacgg catcgacttc gagctgcgca ggggcgagtg cttcgcgctc
       61 gtgggggagt cggggtcggg caagtcgatg accgcgctct cgctaatgcg cctcctgccc
      121 gaagccggcc gcatcgcctc cggccgcgtc gcgctcgatg gcatcgacct tctcgcgctg
      181 cccgaggcgg cgatgcgggc ggtgcgcggc cggcgtgtcg ccatggtgtt ccaggagccc
      241 tcgaccgcgc tcaacccggt gctcaccgtc ggccggcaga tcgtcgaggt cctcgaacgg
      301 cacacgaaca agactccgac agtgaatcgt cagacagcga tcgagctgct gcgcgcggtc
      361 ggcatccggg acgccgagcg gcgcttcgac gaatatccgt tccagctatc cggcgggctc
      421 aagcagcgcg caatgatcgc cgcggcgctc gccgtggacc ccgaggtgct tattgccgac
      481 gagcccacga ccgcgctcga cgtcaccatc caggcgcaga ttctcgagct gctcgccagg
      541 ctgcaggccg agcgcggcat gacgctcctt ctcatcaccc acgacctcgg catcgtggcc
      601 aggatggcgc accgcatagc agtcatgtac gccggcgaaa tcgtggagat cgcggggcgg
      661 gacgcctttc tgcgcgcccc gcagcacccg tactcgcaga agctcttcgc cgcgcttccg
      721 acgccgcagc ggcgagcgcg cgagctggcc gtgatccgcg ggcaggttcc ggcgctcacg
      781 aagaagttcg accattgccg cttcgccgag cgctgcgact acgtattcga gcgctgccgc
      841 gtggaggcgc cgcggctcct tgagccgtcg cccggccact tcgcgcgctg ccaccttcgg
      901 gagcagggtc ctgcatcccc ccggatggtg ggcgacgcac gcgaggaggg taccgtccga
      961 caggaagcgg gcaaaggcgt actgctcgag gcgaaagacc tcaaggttta cttcccgatc
     1021 cggaaaggcc tcttcaagcg cacggtgggc tacgtgcgcg cggtggacgg cgtgtcgctc
     1081 gagctgcgcg agggacgcac gctcgcgctc gtcggtgaat cgggctgcgg caagacgacc
     1141 accggcaagg ccatcctgca gctcataccc ccgtcaggcg gtaccgtgcg tttcgcaggc
     1201 gaggcgctga ccgggctctc gcgcagccag cttcggccgt ggcgcggcgc gatgcagatc
     1261 gtcttccagg acccctacgg ctctctcaat ccgcggctgc gcgtggcgga gatcctgctc
     1321 gaaggcatgc gcagcctcgg cgtcgggtcg agtgatgcac agaggaccga aaggctgaag
     1381 cagctcctgg ccgaggtggg gcttcccccc gattcgctcg agcgctatcc gcacgagtgt
     1441 tcgggcgggc agcgccagcg catcgccatc gcgcgggcgc tcgcggtgga tccgcggctc
     1501 atcgtgtgcg acgagccgac gagtgccctg gatgtctcgg tgcaggcgca gatcctcaac
     1561 ctgctgcgcg cgctgcagga acgactcggc atcgcctacc tcttcatcac ccataacatc
     1621 gcggtcgtgg actacctcgc gcacgacgtc gcggtgatgc accagggcgg gatcgtcgag
     1681 cgcggaacgg ttgacgcggt gctgcggcgc ccgcaacatc cctacaccaa gcagctcctc
     1741 gccgctgtac ccgccatcga ctgatgcgaa tctgctggct catcgcgctc gcgctcgtca
     1801 gcccggcatt gcacgccgcg catgcgtact cgcagttcgg cgacgtcaaa tacccgccgg
     1861 gcttcaggca tttcgagtgg gtgaatccgg atgcgcccaa gggcggcgcg atcgagctcg
     1921 tgccgccgct tcgcatcacc aacttcgaca agtacaaccc cttcacgctg aagggaacgg
     1981 cgccgcccgg cacgggactt ctcttcgaga gcctgcttac cggaaccatg gacgagccga
     2041 cgacggctta cggcctgctt gccgaggacg tcgaggtcgc gcccgacggc ctgtcggcga
     2101 gcttccgcat caatccggcg gcgcgctttc acgacggcac gccggtgacc gcggccgacg
     2161 cgaagcacag cttcgacatg ctgatgagca agcaggcggc gccgcagtac cgcgtgatct
     2221 acggcgacat aaagcaggca gtagtgctcg gcccccgcat gatccgctac gacttcaagc
     2281 gtccgaacgc ggagcttccg ctcatggcgg gcggcctgcc ggtgttcagc cgcgcctggg
     2341 gcgcgggcaa gccgttcgac caggtgg
//
LOCUS       DASUNT010000236         2642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_32126, whole genome shotgun sequence.
ACCESSION   DASUNT010000236 DASUNT010000000
VERSION     DASUNT010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32126"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFX94_04725"
     CDS             <1..200
                     /locus_tag="VFX94_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025211.1"
                     /note="ligates
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate to
                     UDP-N-acetylmuramic acid for reincorporation into
                     peptidoglycan; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEX5767522.1"
                     /translation="SSGLGWDARSLFALLGARARCEDDLDALIGAVAAEARAGDHVVI
                     MSNGGFGGIHEKLLARLSARL"
     gene            175..735
                     /locus_tag="VFX94_04730"
     CDS             175..735
                     /locus_tag="VFX94_04730"
                     /inference="COORDINATES: protein motif:HMM:NF017538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HEX5767523.1"
                     /translation="MLVYLHGFNSSPESDKAQVLQSYMAGRGLAHEYACPTLPPLARD
                     ALRAIEPLLWKNPCFVGSSLGGYYATHLAEKHGLKAVLINPAIDPHVGLRAFLGPQKN
                     LHTGEPYELTEAHLRDWERLYVPRITPGRYLLMVETGDEVLDYRRAVERYAGAEQVVI
                     PGGDHSLQSFPQHLERILEFSRAPSL"
     gene            719..2590
                     /locus_tag="VFX94_04735"
     CDS             719..2590
                     /locus_tag="VFX94_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX5767524.1"
                     /translation="MHLLYEEDGELKTGTVLERSPASFHVRSPHGRRSKIKAANVLLA
                     FERPEPAVLLDEAESFSRELETDFLWECSGAREFGFEELAREYVGREPTPAEAAGVLM
                     KLHSAPMYFYRRGRGRFQAAPEATLKLALAAVEKKKRMQEQVAAWAEELAGGQCPAEV
                     AELKDELLYASDRNKPQAWALEQACAKTGLSAARVFERCGLLSDSHDYHLKRFLHEFF
                     PGGTAFPPHEVPRAGEDLPRAALEAFSLDDIGTTEIDDAFSVQRTPAGTRVGIHIAAP
                     GLGFAPGSALDAISRERLSTAYMPGRKFTMLPDDVIEMFSLDHGDELPAVSLYVDLDA
                     DYAVRARHTRFERVPIVANLRHAEYDVLNEAFEKEETKGLAFEAELKTLWRAALALEA
                     RRGRPGAGSAGLDYTFYVENDRVRIVPRKRGAPLDKLVAEMMILVNSAWGELLAERDV
                     AAIYRVQSTGKVRFGVHPEPHEGLGLKCYAWMSSPLRRYVDLVNQWQLAAALAGRKPS
                     FARNSDTLLAALRAFEVTAARYDEHQRAMETYWSLRWLLQETRTRLEAVVLRENLVRV
                     QGLPLVTRVSAAPTLEPGTLVRMEVTGVDLVELTLSCVYRETLGKVQDATEEAGQKA"
BASE COUNT          454 a          843 c          892 g          453 t
ORIGIN      
        1 atagctcggg cctcggctgg gacgcgcgct cgcttttcgc tctgctcggg gcgcgggcgc
       61 gttgcgaaga cgacctcgac gccttgatcg gcgcggtcgc cgcggaggcg agggcggggg
      121 accacgtagt catcatgtcc aacggcggct tcggcggaat tcacgagaaa ctgcttgctc
      181 gtctatctgc acggctttaa ctcctcgccg gagtcggaca aggcgcaggt cctgcaaagc
      241 tacatggccg ggcgcggcct cgcgcacgaa tacgcttgtc ccaccttgcc gccgttagcg
      301 cgcgacgctc tgcgggcaat cgagccgttg ctttggaaga atccttgctt tgtaggctcc
      361 tcgcttggcg gctactacgc gacccatctg gcggagaagc acgggctcaa ggcggtgctc
      421 atcaaccccg cgatcgatcc acatgtcggc ctgcgcgcct ttctcggacc gcagaagaac
      481 ctgcacacgg gcgagccgta cgagctgacc gaagcgcacc ttcgcgactg ggaaaggctc
      541 tatgtgccgc gcattacgcc tggccgctac ttgttgatgg ttgaaaccgg cgacgaggtg
      601 ctcgactatc ggcgggcggt cgagcgctac gcgggagccg agcaggtggt gatccccggc
      661 ggggatcaca gcctgcagag ttttccgcaa catctcgaac gcatactcga gttttcgcgt
      721 gcaccttctc tatgaggaag atggcgagct gaaaaccggc accgtgctcg agcgctcgcc
      781 ggcgtctttc cacgtccgga gtccgcacgg ccgccggtcg aaaatcaagg ccgccaacgt
      841 actgcttgcc tttgagcggc ccgagcccgc cgtgctcctc gacgaggcgg aaagcttctc
      901 cagggagctc gaaacggatt tcctgtggga atgcagtggc gcgcgcgagt tcggcttcga
      961 ggagcttgcg cgcgagtacg tcgggcgcga gcccacgccg gccgaggcgg cgggggtgct
     1021 gatgaaactg cattcggcgc cgatgtattt ctaccgccgt gggagggggc gtttccaggc
     1081 ggcacccgag gcgacgctca agctcgcact cgccgcagtg gagaagaaaa agcgcatgca
     1141 ggagcaggtt gccgcctggg cggaagagct ggcgggcggc cagtgtccgg ccgaggtggc
     1201 ggagttgaag gacgagctcc tctacgcgtc cgatcgcaac aagccacagg cctgggcgct
     1261 ggagcaggct tgcgcgaaga cgggcttgtc ggcggcgcgg gttttcgagc gctgcggcct
     1321 gctttccgac agccacgact accacctcaa gcgctttttg catgagtttt tcccgggcgg
     1381 cacggctttt ccgccgcacg aggtgccgcg tgcgggggaa gacctgccgc gcgcggcgct
     1441 cgaggccttc agcctcgacg acatcgggac caccgagatc gacgacgcgt tctcggtgca
     1501 gcgcaccccg gccggaaccc gcgtcggcat ccatatcgcg gcgccggggc tcggcttcgc
     1561 cccgggctcg gcgctcgacg ccatttcgcg ggagcgcctt tcgaccgcgt acatgcccgg
     1621 gcgcaagttc accatgctgc ccgacgacgt gatcgagatg ttctcgctcg atcacggcga
     1681 cgaactgcct gccgtgtcgc tctacgtgga tctcgacgcg gactacgcag tgcgcgctcg
     1741 tcatacccgc ttcgagcgcg tcccgatcgt cgcgaacctg cgccacgccg agtacgacgt
     1801 actcaacgaa gccttcgaaa aggaggaaac caaagggctc gcgttcgaag cggagctgaa
     1861 aacgctatgg cgggccgcgc tcgcgctgga ggcgcgccgc ggacggcccg gcgccgggag
     1921 tgcagggctc gactacactt tttacgtcga gaacgaccgt gtgcgtatcg tgccgcgcaa
     1981 gcgcggcgca ccgctcgaca agctggtcgc cgagatgatg attctggtca actcggcctg
     2041 gggtgagctc ctggccgaac gcgacgtagc cgccatttat cgcgtgcaat cgaccggcaa
     2101 agtgcgcttc ggcgtacatc ccgagccgca cgaaggcctc ggcctcaagt gctacgcctg
     2161 gatgagctcg ccgttgcgcc gctacgtcga cctcgtcaac cagtggcagc tcgccgcggc
     2221 gctcgctggc cgcaagccgt ccttcgcgcg caattccgac acgctgctcg cagcgctgcg
     2281 ggcgttcgag gtcaccgcgg cgcgctacga cgagcaccag cgggcaatgg agacctactg
     2341 gagcttgcgc tggctgctgc aggagacgcg cacgcggctt gaagctgtgg tgctgcgcga
     2401 gaatctggtc cgagtccagg gcctgcccct cgttacgagg gtgagcgccg cgccgacact
     2461 ggaaccgggc acgctggtgc gcatggaagt gaccggggtc gatctggtgg agttgacact
     2521 ctcgtgcgtc taccgcgaaa cgctcggcaa agtgcaggat gcaactgagg aggcgggcca
     2581 aaaagcctag aatataaacg gcttgtcgga ctgatgtgag aatgcacttg atattgccga
     2641 ga
//
LOCUS       DASUNT010000237         2268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1084910, whole genome shotgun sequence.
ACCESSION   DASUNT010000237 DASUNT010000000
VERSION     DASUNT010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084910"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VFX94_04740"
     CDS             complement(<1..303)
                     /locus_tag="VFX94_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEX5767525.1"
                     /translation="MDWSEIFGLSVSPAELIIRGTVMYLFLFLLFRVVIRRRVGSVGM
                     ADILVLVIIADAAQNGMSGEYRSVTEGAILVGTIIAWNVILDWLTYKSARLQKILEP"
     gene            complement(305..529)
                     /locus_tag="VFX94_04745"
     CDS             complement(305..529)
                     /locus_tag="VFX94_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767526.1"
                     /translation="MQFARVYYHPSIHSPTRVEAADLYFSGGRPLLVLTWRRKNGERV
                     PDECIELDPARLKRASSNGTIYRYEGQVQR"
     gene            794..2152
                     /locus_tag="VFX94_04750"
     CDS             794..2152
                     /locus_tag="VFX94_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5767527.1"
                     /translation="MPHLLLVDDDPEALEWLSEFARSEGFTVSQADSLRAARIHLSRL
                     QPDVLLTDLQLPDGKGVELVNDLESRESTEVVLITGHASVESAVEALRLGASDYLVKP
                     VDMDRLKAILRRVPRTGELRAEIGELRDELRKLGRFGHILGSSPPMQKLYDQLGRVAP
                     TSATVLLIGESGTGKELAAQTVHDLSRRKKSPFLPLNCGAVSPNLIESELFGHEKGSF
                     TGADRQHKGYFERAHGGTLFLDEITEMPQELQVKLLRVLETGTITRVGTTTPISADVR
                     VLAATNRSPEQAVADGKLREDLYHRLNVFPIAMPPLRERGTDIELLAEHFLALLNKQE
                     SASKKFAPATIAALYAHSWPGNVRELKNYVQRAFILADDVIDVNLAPAAVAAPESAPL
                     LSVRVGTTLEEVSRRLIEATLAECGSKRKAADMLGISLKTLYNRLAAYKSGSAVEERD
                     AA"
BASE COUNT          403 a          759 c          757 g          349 t
ORIGIN      
        1 cggctcgagg atcttctgca ggcgcgcgga cttgtaggtc agccagtcca ggatcacgtt
       61 ccacgcgatg atggtgccga ccaggatggc gccctcggtg accgagcggt actcgcccga
      121 catgccgttc tgcgcggcgt cggcgatgat cacgagcacg aggatgtcgg ccattccgac
      181 cgagccgacg cgccggcgga tgacgacgcg aaagaggagg aagaggaaga gatacatcac
      241 ggtgccgcgg atgatgagct ccgccggcga cacgctcagg ccgaaaattt cgctccagtc
      301 catgtcatct ctgcacttgg ccctcgtagc gataaatcgt gccgttgctg ctcgcgcgct
      361 tcagcctggc gggatccagc tcgatgcact cgtcgggcac gcgctcgccg ttcttgcgcc
      421 gccacgtgag tacgaggagc ggcctgccgc ccgagaagta caggtcggcg gcctccaccc
      481 gggtcgggga gtggatcgaa gggtggtaat agacgcgcgc gaactgcatg cgtcattgcg
      541 caccgcaatt tacggaccgg aataccgccg cgggcgaagc tgcgggggga aggagggagg
      601 gtcgcaacca gcgcccgggc ggaatgccgg cggtcatgca cggcgcgtgc ccgctcgccg
      661 ctgacggggc ggcacgacaa cgcgcgcaca ccgccggttg cgccttgttt taaaatcaag
      721 cacctgtggc gctcgtcacg gctggtatgc atgttgcgtg ccgtttttac aacttttgct
      781 tgtaaggcct tccatgcccc atctgctgct ggtagacgac gatcccgaag cgctcgagtg
      841 gctgtccgag ttcgcgagga gcgaaggctt cacggtatcg caggccgatt cgctgcgtgc
      901 ggcgcgcatc cacctgtcgc ggctgcagcc cgacgtgctg cttaccgatc ttcagctccc
      961 ggacggaaaa ggcgtcgagc tcgtgaatga cctcgaaagc cgcgaatcga ccgaggtggt
     1021 gctgatcacg gggcacgcga gcgtggagag cgcggtcgag gccctgcggc tgggcgcgag
     1081 cgactacctg gtgaagccgg tggatatgga caggctcaag gcgatcctgc gccgggtgcc
     1141 gcgcactggc gagctgcgcg cggagatcgg cgagctgcgc gacgagttgc gcaagctggg
     1201 ccgcttcggc cacatcctgg gctcctcgcc gccgatgcag aagctctacg accagctcgg
     1261 ccgcgtcgct cccacctcgg ccaccgtgct gctcatcggc gaaagcggca ccggcaagga
     1321 gctcgccgcg cagaccgtgc acgatctgag ccggcggaag aagtcgcctt tcctgccgct
     1381 caactgcggc gcggtatcgc cgaatctcat cgagagcgag ctcttcggcc acgagaaggg
     1441 ctccttcacg ggcgcggacc gtcagcacaa gggctatttc gagcgtgccc acggcggcac
     1501 gctcttcctc gacgagatca ccgagatgcc gcaggagctg caggtgaagc tgctgcgcgt
     1561 gctggagacg ggcacgatca cccgcgtcgg gaccaccacg cccatcagcg ccgacgtgcg
     1621 cgtgctcgcc gcgaccaacc gcagccccga gcaggcggtc gcggacggca agctgcgcga
     1681 ggacctctac caccggctca acgtgttccc gatcgcgatg ccgccgctgc gcgagcgggg
     1741 aaccgacatc gagctcctcg cggaacattt cctcgccctc ctcaacaagc aggagagcgc
     1801 ctcgaagaag ttcgcgcccg caaccatcgc cgcgctctac gcccacagct ggccgggaaa
     1861 cgtgcgcgag ctcaagaact atgtgcagcg cgcattcatc ctcgccgacg acgtgatcga
     1921 cgtgaacctg gcgcctgcgg ccgtggccgc gccggagtcc gcgccgctac tctcggtgcg
     1981 cgtcggcacg acgctcgagg aggtgagccg gcggttgatc gaggcgacgc tcgccgagtg
     2041 cggctccaag cgcaaggcgg ccgatatgct cggcatcagc cttaagacgc tgtacaaccg
     2101 cctcgcggcg tacaagagcg gttccgccgt cgaggagcgt gacgccgcct agcgcggcat
     2161 aaacattgct ggtccgttcg cgcatccact taccggagga cacaccatgg ccgaccccac
     2221 aagcacccgc agccccggcg cggaaacgat tcagcgcgtc gccgacac
//
LOCUS       DASUNT010000238         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_32557, whole genome shotgun sequence.
ACCESSION   DASUNT010000238 DASUNT010000000
VERSION     DASUNT010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32557"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1113
                     /locus_tag="VFX94_04755"
     CDS             70..1113
                     /locus_tag="VFX94_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5767528.1"
                     /translation="MTQHRILVVDDTPANVKLLSDLLSVKGYQVSTAVNGEQALDKVA
                     TDKPDLVLLDVMMPGMSGYDVCKRIRANPETALLPVVMCTSLDPNQERVKGIEAGADD
                     FLSKPVNQPELFARVRSLLRIKGLQDERLGRLKSFFSPQLAEAIAAGKGEELLKTHRR
                     EITVEFFDLRGFTHFTESAEPEEVMELLRAYHSALGKLVLEHQGTLERFAGDAVMVFF
                     NDPVPIAKPAEHAVRTALAMHEAFVPIARHWEKRGFELALGVGIAQGYATLGAIGFEG
                     RWDYAAIGNVTNLAARLCGEAKAGQILVDRKTMAALDGAFECEPLGPLALKGFSNPVP
                     AFALKNPGQSPNS"
     gene            1115..>2179
                     /locus_tag="VFX94_04760"
     CDS             1115..>2179
                     /locus_tag="VFX94_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5767529.1"
                     /translation="MARAKKCRTDALGRALKERDEALAFQAATDEILTAISRSPSNAK
                     PVFDAIVRNVPRLFGTRYVAVFLMKGDELHLAAAKADPRFEKTHATVFRHFRESFPRP
                     IDWKGFTGRALRSGKVSQIAPIIGNPKATPQAVQLAKDFGYNAMLVAPLVRDGKTIGA
                     IGTTRPEAKLFDARELALFKRFAAQAVIAIENARLFHETKEALERQTATAEILKVISA
                     SPTATQPVFDSIVQKCHALYQDSRVALLLIEENRLVTRASTGSAFEPMPIDRESGIGA
                     SVLEARTVHLPDLEAAAEEYPRLRQLGIKHGYRSGMYAPLMREGRAVGGISVLRRTAG
                     AFSDKDVALLTTFADQAVIAI"
BASE COUNT          392 a          761 c          698 g          328 t
ORIGIN      
        1 cctcggcaag ccgatcaacc tcagggagtt cctcgagacg gtaaaacgac tgctggagaa
       61 atcgagaacg tgacgcaaca ccgcatcctc gtcgtcgacg acacgccggc gaacgtcaag
      121 ctcctctccg acctgctgtc ggtgaagggc taccaggttt cgaccgcggt gaacggcgag
      181 caggcgctcg acaaggtggc gacggacaag ccggacctcg tgctgctcga cgtaatgatg
      241 cccgggatgt caggttacga cgtctgcaag cgcatccgcg cgaaccccga aaccgcgctc
      301 ctgccggtcg tgatgtgcac ctcgctcgat ccgaaccagg agcgcgtcaa gggcatcgag
      361 gcgggcgcgg acgattttct ttccaagccg gtgaatcagc ccgagctctt cgcgcgcgtg
      421 cgctcgctcc tgcgcatcaa gggcctgcag gacgagcgcc tcgggcgcct gaaaagcttc
      481 ttctcgccgc agctcgccga ggcgatcgcc gccggcaagg gcgaagagct tctcaagacc
      541 catcggcgcg agatcacggt ggagtttttc gacctgcgcg gcttcacgca cttcaccgag
      601 agcgccgagc ccgaggaggt gatggagctc ctgcgcgcct accactcggc gctcggcaag
      661 ctggtgctcg agcaccaggg cacgctcgag cgcttcgccg gcgacgcggt aatggtgttc
      721 ttcaacgacc cggtgccgat cgcaaagccg gccgagcacg ccgtgcgcac cgcgctcgcg
      781 atgcacgaag ctttcgtccc gatcgccagg cactgggaaa agcgcggctt cgagctcgcg
      841 ctcggcgtcg gcatcgcgca gggctacgcc acgctcggcg cgatcggctt cgaggggcgc
      901 tgggactacg ccgcgatcgg caacgtcacc aacctcgccg cgcgcctgtg cggcgaagcg
      961 aaggccggcc agatcctcgt cgaccgcaag accatggccg cgctcgacgg cgccttcgag
     1021 tgcgagcccc tcggcccgct cgcgctcaaa ggcttctcca accccgtccc ggcgttcgcg
     1081 ctgaaaaatc cgggtcaaag cccgaattcc tgacatggca cgcgcgaaga aatgccgcac
     1141 cgacgcgctc ggccgcgcgc tcaaggagcg cgacgaggcg ctcgccttcc aggccgcgac
     1201 cgacgagatc ctgaccgcga tcagccgctc gccctcgaac gcgaagccgg tgttcgacgc
     1261 catcgtgcgc aacgtaccgc ggctcttcgg cacccggtac gtcgccgtgt tcctcatgaa
     1321 gggtgacgaa cttcacctcg ctgccgccaa ggcggacccc cggttcgaaa agacgcacgc
     1381 caccgtcttt cgccacttcc gcgagtcctt cccgcggccg atcgactgga agggattcac
     1441 cggcagggcg ctgcgctcgg gcaaggtgtc gcagatcgcc cccatcatcg gcaacccgaa
     1501 agcgacgccg caggcggtgc agctcgccaa ggacttcggc tacaacgcaa tgctcgtcgc
     1561 ccctctggtg cgcgacggca agacgattgg cgccattggc accacgcgcc cggaggcgaa
     1621 gctcttcgac gcgcgggaac tcgccctctt caagcgcttc gccgcgcagg cggtgatcgc
     1681 gatcgagaac gcgcgcctct tccacgagac gaaggaagcg ctcgagcggc agacggcaac
     1741 tgccgagatc ctgaaagtga tcagtgcttc gccgaccgcg acgcagccgg tcttcgactc
     1801 catcgtgcag aagtgccacg cgctgtacca ggacagccgg gtcgcgctct tgctcatcga
     1861 ggagaaccgg ctagtcaccc gcgcgagcac cggctctgcg ttcgagccca tgccgatcga
     1921 ccgcgagagc ggtatcggcg cctccgtgct ggaggcgcgc acggtccatc tgcccgacct
     1981 ggaggcggcg gccgaggaat atccccgcct gaggcagctc gggatcaagc acggctatag
     2041 atcgggaatg tatgcgccgc tcatgcggga agggcgcgcg gtcggcggca tctcggtact
     2101 gcggcgcacc gccggcgctt tcagcgacaa ggatgtagcg ttgctcacca cgttcgccga
     2161 tcaggcagtg atcgcgatc
//
LOCUS       DASUNT010000239         3247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_208871, whole genome shotgun sequence.
ACCESSION   DASUNT010000239 DASUNT010000000
VERSION     DASUNT010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208871"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VFX94_04765"
     CDS             <1..689
                     /locus_tag="VFX94_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767530.1"
                     /translation="FMLPYSCWEKRAEPPRPPKLLPPLRPPVVFEKPIRLVPFPDKFR
                     FTRFKDEFVKGLEKELDFSTKYLSGLKDTISIFAGGKPGGPTLPGGIVGPALPVERVT
                     NPVLNMHLVDTTVKSQRLDLLRTELLDPTLDEAKRGALETQLKTTEAELANAIVATTE
                     YVAGANLAVEKGTEAGIAMSVATTSLAKLNDLDALTRVETGLTTVGERPATAAEVKTV
                     IGSMLGSRLR"
     gene            691..2109
                     /locus_tag="VFX94_04770"
     CDS             691..2109
                     /locus_tag="VFX94_04770"
                     /inference="COORDINATES: protein motif:HMM:NF039901.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HEX5767531.1"
                     /translation="MQILHRVRRQGWRVGVRSAQEAFAARARLRVAVEHELREALERA
                     FDKAAPGEGVLHIPRLELRLRVASLDRLPEALAEAIERELRLRPPEPRREPQAERLEL
                     LLRYLDSGVLAWHAAHTEPSAAASILRATLRDELLLAVRRGPAHAVACFEPAEQYYFR
                     LLQLLPETRWPEAAAYITASAYATDVSVMRTWLTARAGIPALAAHFLRTARSLEEMIA
                     ASLALPRHEALRLAAAILAALRFPAVLVHEVGGSRASTDRRRNPEEEPVLEMGQPAAR
                     PETPLPSVLPGEQAPIERSLREPERERSGIATFMVGNAGLVLLHPFVPQLFQACGLLE
                     KNRPKKMDSAAALLHWLATGREEVFEFELGFVKLLLGLAPAAPLAVGDGLVGAREREE
                     GEALLRAAIAHWKALGNTTVDGLRVSFLERRGALRDEEHGWRLQLEPESFDVLLGRLP
                     WAIATVKLPWMTRPLYTDWPTP"
     gene            2157..>3247
                     /locus_tag="VFX94_04775"
     CDS             2157..>3247
                     /locus_tag="VFX94_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5767532.1"
                     /translation="MLDGRLKSYFGDAAAHRDPLAIAPPPLDPARSDYARFICAERVP
                     PSERLVILLALIPHVRPQLLDVLWSRNEATQRGFTEFGGMQGANHGGFIPTGETAAFV
                     LAGEDLAARFAMTHLFEPDQFLARQNVVHLSAVAPGESALSGTLVLSREHLHRFTTGL
                     ERRPGFSAEFPARLVRTDMSWDELVLPASTLEQLEEIKRWIVHGGRLLQEWGMGERLR
                     PNFVSLFYGMPGTGKTLAAGLVGRLCGCEVYKLDLSMIVSKYIGETEKNLARVFDLAE
                     HKRWILFFDEADALWGSRTRISDAHDRFANQEVSFLLQRVEEFHGVVILASNLRANID
                     EAFMRRFHSVIHFPMPRAAERLRLWREGFS"
BASE COUNT          483 a         1141 c         1128 g          495 t
ORIGIN      
        1 atttcatgct gccctacagc tgctgggaaa agcgcgccga gccgccgagg ccgccgaagc
       61 tgctgccgcc gctgcggccg ccggtggtgt tcgagaagcc gatccgcctg gtgccgttcc
      121 cggacaagtt ccgcttcacc aggttcaagg acgagttcgt caagggcctg gagaaggagc
      181 tcgacttcag cacgaagtac ctgagcggcc tcaaggacac gatcagcatc ttcgcgggcg
      241 gcaagccggg cggcccgacc ctgcccggcg gcatcgtcgg gcccgcgctg ccggtcgagc
      301 gcgtgaccaa cccggtactg aacatgcacc tcgtcgacac gacggtgaag agccagaggc
      361 tcgacctgct ccgcacggag ctgctcgacc cgaccctgga cgaggcgaag cgcggcgcgc
      421 tcgagacgca gctcaagacc accgaggccg agctcgccaa cgccatcgtc gcgaccacgg
      481 aatacgtcgc cggagcgaac ctggcggtcg agaaagggac cgaggcgggg atcgcgatga
      541 gcgtcgccac caccagcctc gccaagctga acgacctcga tgcgctgacc cgcgtcgaga
      601 ccggcctgac caccgtgggc gagcggccgg ccacggcggc ggaggtaaag accgtgatcg
      661 gcagcatgct gggcagccgc ctgcgctgac atgcaaatcc tgcaccgcgt gcgcaggcaa
      721 ggctggcgtg tcggcgtgcg ttcggcgcag gaggccttcg cggcgcgcgc gcggctgcgc
      781 gtcgcggtgg agcacgagct gcgcgaggcg ctcgagcgtg ccttcgacaa ggcggcgccg
      841 ggggagggcg tcctgcatat tccgcggctc gagctgcggc tgcgcgtcgc gagcctcgat
      901 cggctgcccg aagcgcttgc cgaggcgatc gagcgcgagc tgcggctgcg gccgcccgag
      961 ccgcggcgtg agccgcaggc cgagcgcctg gagctgctgc ttcgctatct cgacagcggc
     1021 gtgctcgcct ggcatgccgc gcacacggag ccgtctgcag cagcctccat cctgcgcgcc
     1081 acgctgcgcg atgagcttct gcttgccgtg cgccgcggac ccgcccacgc tgtggcgtgc
     1141 ttcgagcccg cggagcagta ctactttcgc ctgctccagc tcctgcccga gacgcgctgg
     1201 cccgaagctg cggcgtacat caccgcgagc gcgtacgcca cggatgtcag cgtcatgcgc
     1261 acctggctta ccgcccgcgc cggcattccg gcgcttgccg cgcacttcct ccgcactgcg
     1321 cgttcgctgg aagaaatgat cgccgcgtcc ctggcgctgc cccggcatga agccctccgg
     1381 cttgccgctg cgatcctcgc cgccctgcgc tttcccgcgg tgcttgtaca cgaggtaggc
     1441 gggtctcggg caagcacgga tcgcagacgg aatcctgaag aagaaccggt tctggaaatg
     1501 ggtcagccgg ccgccaggcc ggaaacgccg cttccatctg tcttgccggg agaacaagca
     1561 ccgatcgaac gctcgttgcg cgagcctgag cgcgagcgtt ccgggatcgc gaccttcatg
     1621 gtcggcaacg cggggctcgt gctgctccac ccgtttgtac cgcagctctt tcaggcatgt
     1681 ggtctgctcg agaagaatcg gccgaagaag atggacagcg ctgcggcgct gctccactgg
     1741 ctcgcgacgg gacgcgagga ggtgttcgag ttcgagctcg ggttcgtgaa gctgctcctc
     1801 ggcctcgcac ccgccgcgcc actcgcggtc ggcgacgggc tggtcggcgc gcgggagcgc
     1861 gaggagggtg aggcgctgct gcgcgcggcg atcgcgcact ggaaggcgct cggcaatacc
     1921 acggtcgacg ggctgcgcgt gagcttcctc gagcgccgcg gcgcgctgcg cgacgaagag
     1981 cacggctggc ggctgcagct cgagcccgag agcttcgacg tgctgctcgg gcgcctgccc
     2041 tgggcgatag cgacggtgaa actgccctgg atgacacgac cgctgtacac ggactggccc
     2101 acgccctgag ggcgaacgcc ggggacctcg acgcggacct ggagtggttc acgcgcgtgc
     2161 tggatggccg cctgaagtcc tacttcggcg acgcggccgc gcatcgcgac ccgctcgcca
     2221 tcgcgccgcc gccgctcgat ccggcgcgct cggactacgc gcgctttatc tgcgcggagc
     2281 gcgtgccgcc gagcgagcgc ctcgtcatcc tgctcgcgct gattccgcac gtgcgcccgc
     2341 agctcctcga cgtgctctgg tcgcgcaacg aggccacgca gcgcgggttc accgagttcg
     2401 gcgggatgca aggcgccaac cacggcgggt tcatcccgac cggcgagacc gccgcgttcg
     2461 tgctcgcggg cgaggacctc gccgcgcgct tcgcgatgac gcaccttttc gagcccgacc
     2521 agttcctcgc gcggcagaac gtggtgcatc tctctgcggt tgcgccgggc gagtcggcgc
     2581 tgagcggcac gctcgtgctc tcgcgcgagc acctgcaccg cttcaccacc ggcctcgagc
     2641 gccggcccgg cttcagcgcc gagttcccgg cgcgcctggt gcgcaccgac atgagctggg
     2701 acgagctcgt gcttcccgcc tcgaccctcg agcagctcga ggagatcaag cgctggatcg
     2761 tgcacggcgg ccgcctgctc caggaatggg gcatgggcga gcggctgcgc ccgaacttcg
     2821 tcagcctctt ttacggcatg ccgggcaccg ggaagacgct cgcggcgggg ctcgtgggcc
     2881 ggctgtgcgg ctgcgaggtc tacaagctcg acctcagcat gatcgtgtcc aagtacatcg
     2941 gcgagaccga gaagaacctg gcgcgcgtgt tcgacctcgc cgagcacaag cgctggattc
     3001 tcttcttcga cgaggccgat gcgctctggg gctcgcgcac gcgcatctcc gacgcgcacg
     3061 accgcttcgc caaccaggaa gtgagctttc tcctgcagcg ggtcgaggag ttccacggcg
     3121 tggtgattct cgcctcgaac ctcagggcga acatcgacga ggcgttcatg cgccgcttcc
     3181 actcggtgat ccacttcccg atgccgcgcg cggccgagcg cctgcggctg tggcgcgagg
     3241 ggttctc
//
LOCUS       DASUNT010000240         3422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_471910, whole genome shotgun sequence.
ACCESSION   DASUNT010000240 DASUNT010000000
VERSION     DASUNT010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471910"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VFX94_04780"
     CDS             <1..571
                     /locus_tag="VFX94_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX5767533.1"
                     /translation="LCGSSGANPYACIAAGIASLWGPAHGGANEACLQMLEEIRDVSN
                     VPEFIRKVKDKNSGVRLMGFGHRVYKNYDPRAKLMRETCHEVLNELGLHNDRLFKLAI
                     ALEKIALEDEYFVSRKLYPNVDFYSGIVQRALGIPVSMFTCIFSLARTIGWIAQWQEM
                     ITDPEYKIGRPRQLFKGATRRDVKPIDER"
     gene            576..3404
                     /locus_tag="VFX94_04785"
     CDS             576..3404
                     /locus_tag="VFX94_04785"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX5767534.1"
                     /translation="MLKEFLSNSYLFGGNAAFIEELYEKYLANPQSIAEEWRDYFDRM
                     QDLPGATDKDVAHAPVVESFVQRAKRGEFTSQRTIAAEPVTPERLQVAALLLVTAYRI
                     AGARWATVDPLKRMPRPNIPELEPAYYDLKEADLDQVVNAGSFVGLERVSLRVLVQAL
                     RDTYCRNIGFEYMFISDRAQRQWIQERIEPLRAAPRVTPAEQKRLLQKLTEAEHLERY
                     LHTRYVGQKRFSLEGGESLIPSIDELIQRAGGAGVQEIVIGMAHRGRLNVLVNVLGKM
                     PKDLFLEFEGKQASELPSGDVKYHNGYSSDISTAGGPVHLSLAFNPSHLEIVNPVVEG
                     SVRARQRRRDDKTGDQVLPILVHGDAAFAGQGVVMETLNLSGTRGYGTGGTVHLIVNN
                     QIGFTTSDPRDTRSTIYCTDVAKMVEAPIFHVNGDDPEAVLFTTRLALEFRQTFHKDV
                     VIDIVCFRKLGHNEQDEPAVTQPLMYKKIAQHPGTRRLYADKLVTQGVVREEEPERMI
                     KAYRDTLDAGQQTVSPVLSNFKSKFAVDWAPYLGAKWTDAADTHVPLDQLKRLSERIT
                     GLPEDFRLHPSVARVIEARKQMAAGKLPLDWGMAETLAYASLLSNGYDVRLSGQDSAR
                     GTFAHRHAVLHDQDRERWDQGSYVPLQNISKDQGNFVVIDSVLSEEAVLGFEYGFSTA
                     EPNALVIWEAQFGDFANGAQVVIDQFISSGETKWGRVCGITLFLPHGYEGQGPEHSSA
                     RLERFLQLCAEYNMQICVPTSAAQIFHLLRRQMLRPFRKPLVVFTPKSLLRKKEAASP
                     IGELANGSFQTVIPDVEAPEPKEVKRIVACSGKVYYDLAAKRHESRRNDVAIIRVEQL
                     YPFPHKQFEAEMKRYPNASEVVWCQEEPQNQGAWYQSGHYFRQNMREDQKLYYAGRPA
                     SAAPAGGYLARHNERQRTLVEQALGNYK"
BASE COUNT          646 a         1127 c         1112 g          537 t
ORIGIN      
        1 cctatgcggc tcctccggcg ccaaccccta cgcctgcatc gccgccggca tcgcctcgct
       61 ctggggtccg gcacacggcg gcgccaacga ggcctgcctg cagatgctgg aggagatccg
      121 cgacgtctcc aacgtgccgg agttcatccg caaggtgaag gacaagaact cgggcgtgcg
      181 gctgatgggc ttcgggcacc gcgtctacaa gaactacgac ccgcgcgcga agctcatgcg
      241 cgagacctgc cacgaggtgc tgaacgagct cggcctgcac aacgaccgcc tgttcaagct
      301 cgccatcgcg ctggaaaaga tcgcgctcga agacgagtac ttcgtctcga gaaagctgta
      361 tcccaacgtc gatttctatt ccggcatcgt gcagcgcgcg ctcggcatcc cggtgtcgat
      421 gttcacgtgc atcttctcgc tcgcgcgcac catcggctgg atcgcgcagt ggcaggagat
      481 gatcaccgac ccggagtaca agatcggccg gccgcgccag ctcttcaagg gcgccaccag
      541 gcgcgacgtg aagccgatcg acgagagata ggctcatgct gaaggaattc ctgagcaatt
      601 cgtatctctt cggcgggaac gccgctttca tcgaggagct gtacgagaag tacctcgcca
      661 acccgcaatc gatcgctgaa gagtggcgcg actacttcga ccgcatgcag gaccttcccg
      721 gcgccaccga caaggatgtc gcgcacgccc cggtagtcga atcgttcgtt cagcgcgcga
      781 aacggggtga attcaccagt caaagaacca tcgcggccga gccggtcacg cccgagcgcc
      841 tgcaggtcgc ggcgctgctg cttgtcaccg cctaccgcat tgccggggcg cgctgggcca
      901 cggtcgaccc gctcaagcgc atgccgcggc cgaacatccc cgagctcgag cccgcgtact
      961 acgacctcaa ggaagccgac ctcgaccagg tggtgaacgc cggctcgttc gtgggattgg
     1021 agagagtctc tctgcgggtg ctcgtgcaag cccttcgaga cacctattgc cgcaacatcg
     1081 gcttcgagta catgttcatc tccgaccgcg cgcagcgcca gtggattcag gagcgcatcg
     1141 agccgttgcg cgccgcgccg cgggtcacgc ccgccgagca gaagcgcctg ctgcagaagc
     1201 tcaccgaagc cgagcacctc gagcgctacc tgcacaccag gtacgtcggc cagaagcgct
     1261 tctcgctcga gggcggcgag agcctgatcc cgtcgatcga cgagctcatc cagcgcgcgg
     1321 gcggcgccgg cgtgcaggag atcgtcatcg gcatggcgca tcgcgggcgg ctcaacgtgc
     1381 tcgtcaacgt gctcggcaag atgccgaagg acctcttcct cgagttcgag ggcaagcagg
     1441 cctcggagct gccgtcgggc gacgtcaagt accacaacgg ctattcgtcg gacatctcca
     1501 ccgcgggcgg accggtgcat ctttcgctcg ccttcaatcc ctcgcacctg gagattgtga
     1561 acccggtggt cgaaggctcg gtgcgcgcgc gccagcgccg ccgcgacgac aagaccggcg
     1621 accaggtgct gccgatcctg gtgcacggcg atgccgcgtt cgccggccag ggggtggtga
     1681 tggagacgct caacctgtcg ggaacgcggg gatacggcac cggcgggacg gttcacctca
     1741 tcgtgaacaa ccagatcggc tttacgacct ccgacccgcg cgacacccgc tccacgatct
     1801 actgcacgga cgtggcgaaa atggtggagg caccgatttt ccacgtgaac ggcgacgacc
     1861 cggaagcagt gctcttcacg acgcgcctcg cgctcgagtt ccgccagacc tttcacaagg
     1921 acgtggtgat cgacatcgtc tgcttccgca agctcggcca taacgagcag gacgagcccg
     1981 cggtgacgca gccgctcatg tacaagaaga tcgcgcagca ccccggcacg cgtcggctct
     2041 acgccgacaa gctcgtcacg cagggcgtcg tcagggaaga ggagccggag cgcatgatca
     2101 aggcctaccg cgacacgctg gacgcggggc agcagacggt gagcccggtg ctctccaatt
     2161 tcaagtcgaa gttcgcggtg gactgggcgc cctatcttgg cgcgaagtgg acagacgccg
     2221 cggatactca cgtgccgctc gaccagctga agcgtctttc ggagaggatc accgggcttc
     2281 ccgaggactt caggctgcat ccgagcgtcg cgcgcgtaat cgaggcacgc aagcagatgg
     2341 cggccggcaa gctgccgctc gactggggca tggccgagac gctggcctac gcgtcgctgc
     2401 tctcgaacgg ctacgacgtg cgcctttccg ggcaggactc cgcgcgcggc acctttgctc
     2461 accgccacgc ggtgctgcac gaccaggacc gcgagcgctg ggaccagggc agctacgtgc
     2521 cgctgcagaa catctccaag gaccagggga acttcgtcgt gatcgactcg gtgctctcgg
     2581 aggaggcggt gctcggcttc gagtacggct tttccaccgc cgagccgaac gcgctcgtca
     2641 tctgggaggc gcagttcggc gacttcgcga acggcgccca ggtggtgatc gaccagttca
     2701 tctcatcggg cgagaccaag tggggccggg tgtgcggaat cacgctcttc ttgccgcacg
     2761 gctatgaagg acaggggccg gagcactcct cggcgcgcct cgagcgcttc ctgcagctgt
     2821 gcgccgagta caacatgcag atctgcgtgc cgaccagcgc ggcgcagatc ttccacctgc
     2881 tgcgcaggca gatgctgcgg cccttccgca agccgctggt ggttttcacg ccgaagtcgc
     2941 tgctgcggaa gaaggaggcg gcctcgccca tcggcgagct cgcgaacggc tcgttccaga
     3001 cggtaatccc cgacgtagaa gcgccggagc cgaaggaggt gaagcgcatc gtcgcctgca
     3061 gcggcaaggt ctattacgac ctggcggcaa agaggcacga gagccggcga aacgacgtcg
     3121 cgatcatccg cgtggagcag ctttatccct tcccgcacaa gcagttcgag gccgagatga
     3181 agcgctatcc gaacgccagc gaggtcgtgt ggtgtcagga agagccccag aaccaggggg
     3241 cgtggtacca gagcggacac tacttccgcc agaacatgcg cgaggaccag aagctgtact
     3301 atgccggccg cccggcttcc gcggccccgg ccggcggcta cctggcgcgg cacaacgagc
     3361 ggcagaggac attggtggag caggctctag gcaactacaa gtaggaggaa ttcggggtca
     3421 gg
//
LOCUS       DASUNT010000241         3577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_209035, whole genome shotgun sequence.
ACCESSION   DASUNT010000241 DASUNT010000000
VERSION     DASUNT010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209035"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VFX94_04790"
     CDS             complement(<1..274)
                     /locus_tag="VFX94_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5767535.1"
                     /translation="MKVVWTIVKRELGAYFTSPVAYVFLVIFLLLTGFFTFTAGNFFE
                     RGEASLAAFFGWHPWLYLVLVPAVGMRLWAEERRSGTLELLLTMPVA"
     gene            complement(271..1191)
                     /locus_tag="VFX94_04795"
     CDS             complement(271..1191)
                     /locus_tag="VFX94_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5767536.1"
                     /translation="MIRIENLVKTFGPKRAVDGVSFNVERGEVLGFLGPNGAGKSTTM
                     RMITGFMPPTAGRISVGGHDVQQSPLEAKRLIGYLPENAASYPDMTVKGFLDFAAELR
                     GLERAKRKQAVDRVVELCFLGSVLRQSIDTLSKGYKHRTCLAQALIHDPDVLVMDEPT
                     DGLDPNQKHEVRNLIRELGKSKAIVFSTHILEEVDAACTRAIIIDRGRIVANGTPNEL
                     RNMSQLAGAVTLSAHGASEEKLATLGRVERINGAWRIYPHDKTRAPQLAQAVVDLVAR
                     EGWKVEGMYSERGELDEVFRRITLPDTVKQ"
     gene            1275..2225
                     /locus_tag="VFX94_04800"
     CDS             1275..2225
                     /locus_tag="VFX94_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5767537.1"
                     /translation="MRSVVGKLVSALALTLVAGAVSAQPSLKMMIPANPGGGWDQTGR
                     NLAAAMQSAKLVSGVQFDNKGGAAGTIGLAQFVNGSKGDPNAVMIGGMVMVGGIILNK
                     SPVNLTQVTPIARLTSEWEVIVVPASSPYKTMAELVKALKENPGKVAWGGGSAGGTDH
                     ILVGLIAKEVGVDPAKINYVPFKGGGEAIAAILGGHVAAGVSGIGEFSEQIKAGKMRA
                     LAQSSPKKLENIPSLKEQGINVELGNWRGIFGAPGISPAQRDALVKLVRSATETPAWK
                     STLEKLGWEPWFLAGDDYKKFLDEDIKRVGTIIESLGLKR"
     gene            2230..2730
                     /locus_tag="VFX94_04805"
     CDS             2230..2730
                     /locus_tag="VFX94_04805"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX5767538.1"
                     /translation="MRRSGAEIALSLGVLALGIGAAAVTATLPGEAGYAGIGPNFIPG
                     VVSLGLIALGAWLAFEAFSGGWRGALPDDARERGEHPFNGAAFGWITAGLFAQMALIG
                     SAGFVIAAIVLFACVARGFGSRRVARDLAIGVVLALAIYLFFVQALNVNLPAGWLSPI
                     LGGAGI"
     gene            2741..>3577
                     /locus_tag="VFX94_04810"
     CDS             2741..>3577
                     /locus_tag="VFX94_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5767539.1"
                     /translation="MTETLSALASGFGVALQPVNLLWGLIGVTCGTLVGVLPGVGPAL
                     TVAMLLPLTVKLDPTGALIMFAGIYYGAMFGGSTTTILLNTPGESASIATALEGNRMA
                     KAGRGGPALATSAIGSFVAGSIATILLTLLAPLVVDVALKFGPAEFFSLMVFAFVTVS
                     AVLGSSTVRGLTSLFLGLLLGLVGIDSQTGASRFAFGVPELLDGVDVVVLAVGLFAVG
                     EALYVAAYQSRLTETIEKLRGSIWMSLDDWRRSWPAWLRATFIGFPFGTIPAGGTEIP
                     TFL"
BASE COUNT          538 a         1198 c         1218 g          623 t
ORIGIN      
        1 gtgccaccgg catcgtgagg agcagctcga gcgtccccga gcgccgctcc tcggcccaga
       61 gccgcatccc gaccgccggc acgaggacga ggtacagcca cggatgccag ccgaagaacg
      121 cagcgaggct tgcttcgccg cgctcgaaga aattgcccgc cgtgaaagtg aaaaagccgg
      181 tgagcagcag gaagatcacg aggaacacat acgccaccgg cgaggtgaag tacgcgccga
      241 gctcgcgctt gacgattgtc cagacgacct tcattgcttc accgtgtcgg gcagggtgat
      301 gcggcggaac acctcgtcga gttcgccgcg ctcgctgtac atgccttcga ctttccagcc
      361 ctcgcgcgcg acgagatcca ccaccgcctg cgcgagctgc ggcgccctcg tcttgtcatg
      421 cgggtagatg cgccacgcgc cattgatgcg ctcgacccgg ccgagggtgg cgagtttttc
      481 ttcgctcgcg ccgtgggccg atagcgtcac cgcaccggcg agctgcgaca tgttgcgcag
      541 ctcattgggg gtgccgttcg ccacgatcct gccgcggtcg atgatgatcg cccgggtgca
      601 cgccgcgtcc acttcttcaa ggatatgggt cgaaaacacg atcgccttgc tcttgccgag
      661 ctcgcggatg aggttacgca cttcgtgctt ctggttcgga tcgagcccgt cggtcggctc
      721 gtccatgacg agcacgtcgg ggtcgtggat cagcgcctgg gcaaggcacg tgcggtgctt
      781 atagcccttc gacagcgtgt cgatcgactg tctgagcacg gagccgagga agcacagctc
      841 gactacgcga tccaccgcct gcttgcgctt tgcgcgctcc aaaccgcgca gctcggcggc
      901 aaagtcgagg aagcccttta ccgtcatgtc cggatacgag gcggcgtttt ccggcaggta
      961 gccgatcagg cgcttcgcct cgagcggcga ctgctgcacg tcgtggccgc cgacgctgat
     1021 gcggccggcc gtgggcggca tgaacccggt gatcatgcgc atggtggtcg acttgccggc
     1081 gccgttcggg ccgaggaagc cgagcacttc gccgcgctcc acgttgaatg aaacgccgtc
     1141 cacggcccgc ttggggccga acgtcttgac caggttttcg atgcggatca cgggcttacc
     1201 gccagaccat ctttcaaggg ggcggattat cgcttaaatt gcgggtcgaa gcccattccc
     1261 cgaggaggta tttcatgcgt tccgtcgtcg gcaagttggt ttccgcgctg gcgctgacgc
     1321 tcgtcgccgg cgcagtctcc gcgcagccga gcctgaaaat gatgatcccc gcgaaccccg
     1381 gcggcggctg ggaccagacc ggccgcaacc tcgcggccgc gatgcagagc gcgaagctcg
     1441 tctcgggcgt gcagttcgac aacaagggcg gcgccgcggg cacgatcggg ctcgcgcagt
     1501 tcgtgaacgg ctccaagggg gacccgaacg ccgtgatgat cggcggcatg gtgatggtcg
     1561 ggggcatcat cctcaacaag tccccggtga acctgacgca ggtcacgccg atcgcgcggc
     1621 tcacgagcga atgggaagtg atcgtggtgc cggccagctc gccgtacaag acgatggccg
     1681 agctcgtgaa ggcattgaag gagaaccccg gcaaggtggc ctggggtggc ggctcggcgg
     1741 gcggcaccga ccacatcctg gtcggcctga tcgcgaagga agtcggcgtc gacccggcga
     1801 agatcaacta cgtgccgttc aagggcggcg gcgaggcgat cgccgcgatc ctcggcggcc
     1861 acgtggctgc cggcgtatcg ggcatcggcg agttctccga gcagatcaag gcgggcaaga
     1921 tgcgcgcgct cgcgcagtcg agcccgaaga agcttgagaa cattccctcg ctcaaggagc
     1981 agggcatcaa cgtcgagctg ggcaactggc gcggcatttt cggcgcgccc ggcatcagtc
     2041 cggcccagcg cgacgcgctc gtgaaactgg tgcgctcggc caccgagacg ccggcctgga
     2101 agtccacgct ggagaagctc ggctgggagc cctggttcct cgccggggac gactacaaga
     2161 agttcctcga cgaagacatc aagcgcgtag gcaccatcat cgagtcgctc gggttgaagc
     2221 gctaacgcga tgcgccgctc gggcgccgaa atcgcgctgt cgctgggggt gctcgcgctc
     2281 ggcatcggcg cggccgcggt gaccgcgacg ctgccgggcg aagcgggcta cgccggcatc
     2341 ggcccgaact tcattccggg cgtcgtttct cttggcctga tcgcgctcgg cgcctggctc
     2401 gccttcgagg cgttctcggg cggatggcgc ggcgcgctac ccgacgacgc gcgcgagcgg
     2461 ggcgagcatc cgttcaacgg cgccgcgttc ggctggatca cggcggggct tttcgcgcag
     2521 atggcgctca tcggctcggc cggcttcgtc atcgccgcca tcgtgctctt cgcgtgcgtg
     2581 gcgcgcggct tcggcagccg gcgcgtcgcg cgcgatctcg cgatcggcgt ggtgctcgcg
     2641 ctcgcgatct accttttctt tgtccaggcg ctgaacgtca acctgccggc gggatggctc
     2701 tcgcccatcc tcggcggcgc gggcatatag cccccacgcc atgacggaaa cgctgagcgc
     2761 gctcgcttcc ggattcggcg tcgcgctgca gccggtgaac ctgctttggg gcctgatcgg
     2821 cgtcacctgc ggcacgctgg tcggcgtgct gccgggggtc ggaccggcgc tcaccgtggc
     2881 catgctgctt cccctcacgg tcaagctcga cccgaccggc gcgctcatca tgttcgccgg
     2941 catctactac ggcgcgatgt tcgggggctc gactacgacg atcctgctca acacgccggg
     3001 ggaatcggcc tcgatcgcca ccgccctcga aggcaaccgc atggccaagg ccggccgcgg
     3061 cgggccggcg ctcgccactt cggcgatcgg ctcgttcgtc gccggctcga tcgccacgat
     3121 cctgctcacg ctgctcgcgc cgctggtcgt ggatgtcgcg ctcaagttcg gcccggccga
     3181 gttcttctcg ctcatggtgt ttgcgttcgt caccgtgtcg gcggtgctcg gcagctcgac
     3241 cgtgcgcggg ctgacgagcc ttttcctcgg cctgctgctc ggcctggtcg gcatcgacag
     3301 ccagaccgga gcttcgcgct tcgccttcgg cgtgcccgag ctgctcgacg gagttgatgt
     3361 ggtcgtgctc gcggtggggc ttttcgccgt cggcgaggca ctctacgtgg cggcctacca
     3421 gagccggctc accgagacga tcgagaagct gcgcggctca atctggatgt ccctcgacga
     3481 ctggcggcgc tcctggccgg cgtggctgcg cgccaccttc atcggctttc cgttcggcac
     3541 catcccggcg ggcgggaccg agatcccgac attcctc
//
LOCUS       DASUNT010000242         2086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1261744, whole genome shotgun sequence.
ACCESSION   DASUNT010000242 DASUNT010000000
VERSION     DASUNT010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261744"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VFX94_04815"
     CDS             <1..920
                     /locus_tag="VFX94_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX5767540.1"
                     /translation="GTNLGAASPVSIGGSAQKDEKKELDTMTKKVTNDAVAYIRSLAQ
                     LRGRNADWGEKAVREAVSLPAEEAVKLKVIDHVATDVPDLLKKLGKANAQIVQVEVDW
                     RTQILAVVTNPSVAYILILLGIYALIFEFTNPGLILPGVVGAICILLALYSFHLLPVN
                     YAGLALMLLGIAFMVAEAFLPAFGSLGIGGLIAFVLGSIILIDTDRVPSGFDIPYPLI
                     GGVAVASAAFVFLVVGMAVRQRRRPVLTGREHMIGAVAEALEDFEREGWARVHGESWR
                     VRADQPVRSGERLRVKAIDGLILTVEVER"
     gene            917..1666
                     /locus_tag="VFX94_04820"
     CDS             917..1666
                     /locus_tag="VFX94_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006766307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX5767541.1"
                     /translation="MIQFILGALVLLIIIAALTIKILREYERAVVFFLGRVQRVFGPG
                     IIFIIPGVQQIVRVDLRIRVFDVPPQDIITRDNVSVKVNAVVYFRVVDPRNAIIQVAN
                     FLEATSQLAQTTLRAVLGKHELDALLAEREKLNIDIQTVLDQQTDAWGIKVSNVEIKH
                     VDINESMVRAIARQAEAERERRAKVIHAEGELQASEKLLQAAQMLAQKSEAMQLRYLQ
                     TLTQIAGDKASTIVFPIPMELIGPLLGKKSQ"
     gene            complement(1632..1850)
                     /locus_tag="VFX94_04825"
     CDS             complement(1632..1850)
                     /locus_tag="VFX94_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767542.1"
                     /translation="MENIVRLAEYRKRRKPARRAAAPAELTAQYFCTRCDADEFKLGA
                     AGMVHCAHCGALMRNLLVTATSSREADQ"
BASE COUNT          376 a          643 c          705 g          362 t
ORIGIN      
        1 cggggacgaa cctcggggcg gcgtcgccgg tgtcgatcgg cggctctgcg cagaaggacg
       61 agaagaaaga actcgacacc atgaccaaga aggtcaccaa cgatgccgtg gcttatataa
      121 gaagcctggc gcagctgcgc ggccgcaacg ccgactgggg cgagaaggcg gtgcgcgagg
      181 cggtgagcct gccggccgag gaggcggtga agctcaaggt catcgaccac gtcgcgacgg
      241 atgtcccgga cttgctgaaa aagctcggca aggcaaatgc gcaaatcgtc caggtcgagg
      301 tcgactggcg aacccagatc ctcgccgtcg tcaccaaccc gagcgtggcc tacatcctga
      361 ttctgctcgg aatttacgcc ctgatcttcg agttcaccaa tcccggcctg atcctgcccg
      421 gcgtggtcgg cgcgatctgc atattgctcg cgctctattc gttccacctg ctgccggtga
      481 actatgccgg cctggccctg atgctccttg gcattgcgtt catggtggcg gaggccttcc
      541 tgccggcgtt cggctcgctc ggcatcggcg ggctgatcgc gttcgtgctc ggctcgatca
      601 tcctcatcga caccgatcgc gtgccgagcg gcttcgacat cccttacccg ctgatcggcg
      661 gggtggcggt ggcgagcgcg gcgttcgtgt tcctggtggt cggcatggcg gtgcgccagc
      721 ggcgccggcc ggtgctcacc gggcgggagc atatgatcgg tgccgttgcc gaggcgctcg
      781 aggacttcga gcgcgaaggc tgggcgcgcg tgcacggcga gagctggcgc gtgcgggccg
      841 atcagccggt acgcagcggc gagcggctgc gcgtgaaggc gatcgacgga ctgattctca
      901 cggtggaggt ggaaagatga tccagttcat cctgggcgcg ctggtgctgc tcatcatcat
      961 tgccgcgctg accatcaaga tcctgcgcga atacgagcgc gcggtggtgt ttttcctcgg
     1021 gcgcgtgcag cgcgtgttcg gcccgggcat catcttcatc atccccggcg tgcagcagat
     1081 cgtgcgcgtg gacctgcgca tccgggtgtt cgacgtgccg ccgcaggaca tcatcacgcg
     1141 cgacaacgtc tcggtgaagg tgaacgcggt ggtctacttc cgcgtggtcg acccgcgcaa
     1201 cgcgatcatc caggtggcga acttcctcga ggccaccagc cagctcgcgc aaaccaccct
     1261 gcgggcggtg ctcggcaagc acgagctcga cgcgctgctc gccgagcgcg agaagctcaa
     1321 tatcgacatc cagacggtgc tcgaccagca gaccgacgcc tgggggatca aggtgtcgaa
     1381 cgtggagatc aagcacgtcg acatcaacga gtcgatggtg cgggccatcg cgcgccaggc
     1441 cgaggccgag cgcgagcgcc gcgccaaggt gatccatgcc gaaggggagc tgcaggcctc
     1501 cgagaagctg ctgcaggccg cgcagatgct cgcccagaag agcgaggcga tgcagctgcg
     1561 ctacctgcag accctcacgc agatcgccgg cgacaaggcg tcgacgatcg tatttcccat
     1621 cccgatggag cttattggtc cgcttctcgg gaagaagtcg cagtaaccag aagattacgc
     1681 atcagcgcgc cgcaatgggc gcaatggacc atgccggcgg cgcccagctt gaactcgtcg
     1741 gcgtcgcagc gcgtgcagaa gtactgcgcg gtgagctcgg cgggcgcggc ggcgcgcctg
     1801 gcgggcttgc ggcgcttgcg gtactcggcg aggcggacga tgttttccat caatgacccg
     1861 gtggtacgca atttgattct ttttatgcgc tcgcgttctg aagcggcgta tattcatttg
     1921 aagccggaag aaatcgacac gctgtagctt aggggggaca acgctcagac ctttcagcga
     1981 ggagagaagc gatgacccag ccgcaagccg aatttcttga cctgtaccgg gccggtctca
     2041 agaccagcgc cgacctgatg aaagcctccc tgcagaacgc agagcg
//
LOCUS       DASUNT010000243         2928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_734624, whole genome shotgun sequence.
ACCESSION   DASUNT010000243 DASUNT010000000
VERSION     DASUNT010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734624"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFX94_04830"
     CDS             complement(<1..446)
                     /locus_tag="VFX94_04830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5767543.1"
                     /translation="MTPRISVVVPAFNEERLLAGSLAAMRDAMRVFEEAGWRTELIVC
                     DNNSTDRTAEIARAASARVVFEPVNQIARARNAGAAHASGDWLLFIDADSYPSLDLFR
                     DVVSRIKSPDTLAGGATVDFDTDERLVRWWIAAWNFMSRRMRLAAGS"
     gene            complement(443..952)
                     /locus_tag="VFX94_04835"
     CDS             complement(443..952)
                     /locus_tag="VFX94_04835"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5767544.1"
                     /translation="MILRVYRPSDRDAVIALFRAFMQELTAPGRRAEFEAYIARAIRE
                     ELGRIEQYYFGREGQGFWVAWDAGLVGMVGIERHAVDAGELRRMAVDPAHRRKGVGRA
                     LLAMAEAFCRECGYRRVVLNTTELQGAARSLYQSSGYRLAREERDTQASHKSVGAGYV
                     RYYYEKQLA"
     gene            complement(949..1353)
                     /locus_tag="VFX94_04840"
     CDS             complement(949..1353)
                     /locus_tag="VFX94_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX5767545.1"
                     /translation="MRIDGTCHCGRITYEADIDPERVGVCHCTDCQTLSGSAFVVYLP
                     VPKESLRLRGEPKVYVKTSESGTRRAQAFCADCGSRLYASAERDPQVFNLRIGTVRQR
                     ASLPPRIQIWCRSALPWVMDLASVRQHPKQPM"
     gene            complement(1363..1908)
                     /locus_tag="VFX94_04845"
     CDS             complement(1363..1908)
                     /locus_tag="VFX94_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767546.1"
                     /translation="MDKAQITQSIAQAVLLDPLSEGMNLVRRYQEVRGFRSYVTERAR
                     LLAPIAVLVGLTSLACAAATVMYIGGLQPILVLLSLLLAPVILAGSAFVQVYMYLSWL
                     ENRALAKALRHPIQRGGPIRTQLRKAGIDLGAVPRVPWLLTIVFLFLPLSMLATIAPP
                     IGIALFLLHAAAPFVFARLDR"
     gene            complement(1956..2261)
                     /locus_tag="VFX94_04850"
     CDS             complement(1956..2261)
                     /locus_tag="VFX94_04850"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HEX5767547.1"
                     /translation="MKLSQALPELVADLEMGLLNLGRPDLVEQLKEASVSRWTYDEFA
                     DTAYLQIGADPVDMMHVQRISLYDELGVSVDADPRGRLCGIEVLEGRRVALRLERAS"
     gene            complement(2258..2632)
                     /locus_tag="VFX94_04855"
     CDS             complement(2258..2632)
                     /locus_tag="VFX94_04855"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX5767548.1"
                     /translation="MITHKGGCHCGRVRFEVLAPPRISVVDCNCSMCAKTGYLHLIVP
                     NAQFRLVAGSEVLAEYKFNTGTARHLFCSVCGIKSFYVPRSHPDGFSVNARCLDAGTV
                     EQMTVVPKDGKNWEKTYASKSR"
BASE COUNT          520 a          963 c          967 g          478 t
ORIGIN      
        1 gacccggcgg cgaggcgcat gcgccggctc atgaagttcc acgccgcgat ccaccagcgc
       61 acgaggcgct cgtcggtgtc gaagtccacc gttgcgccgc ccgcgagcgt atccgggctc
      121 tttatccgcg agacaacgtc ccggaacaga tccaggctcg gataggagtc tgcatcgatg
      181 aaaagcagcc aatcgccgct cgcatgggca gcaccggcgt tgcgcgcccg cgcaatctgg
      241 ttgaccggct cgaacaccac gcgcgcgctt gcggcccgcg cgatctcggc ggtgcggtcc
      301 gtggagttgt tgtcgcacac gatgagctcg gtcctccagc cggcttcctc gaagacgcgc
      361 atggcgtcgc gcatcgcggc aagcgatccg gcgagaaggc gctcctcgtt gaacgccggc
      421 acgacgaccg aaatccgggg cgtcacgcga gttgcttttc gtagtaataa cgcacgtagc
      481 ccgcgccgac ggacttgtgc gaagcctgcg tatcgcgctc ctcgcgcgcg aggcggtagc
      541 cgctcgattg gtagaggctg cgcgccgcgc cctgcagctc ggtggtgttg aggaccacgc
      601 gacgataccc gcactcgcgg cagaaagcct ccgccatggc gagcagcgcg cggccgacgc
      661 cctttcggcg atgagccggg tctaccgcca tgcgcctcag ctcgcccgcg tccacggcgt
      721 gtctttcgat tcccaccatg ccgaccaggc ccgcgtccca ggcgacccag aagccctggc
      781 cttcgcggcc gaagtagtac tgctcgatcc tgccgagctc ttcgcggatc gcgcgggcga
      841 tgtaggcctc gaactccgcg cgccgtccgg gcgcagtcaa ctcctgcatg aatgcgcgga
      901 acagcgcgat caccgcgtcg cggtcggagg gccggtatac cctcaagatc acatgggctg
      961 cttggggtgc tgcctgactg aggcgagatc catgacccac ggcagcgcgg agcggcacca
     1021 gatctgaatc ctgggcggga gtgaagcgcg ctggcgcacc gtgccgatgc gcaagttgaa
     1081 cacctgaggg tctcgttccg cggaagcgta gaggcggctg ccgcaatcgg cgcagaaggc
     1141 ctgtgcgcgg cgggtgccgc tctcggaggt ttttacgtac accttcggct cgccgcgcag
     1201 ccggagcgac tccttcggca caggcaggta gacaacaaag gcagagccgg aaagcgtctg
     1261 gcaatcggtg cagtggcaca caccaaccct ttcagggtcg atgtccgcct cgtacgtgat
     1321 ccggccgcag tgacaggtgc cgtctattcg cacccgccta gtttagcggt cgaggcgggc
     1381 gaaaacgaac ggcgcggcgg cgtgaaggag gaagagcgct atcccgattg gcggcgcgat
     1441 ggtcgccagc atcgacagcg gcaggaagag aaaaacgatg gtgagaagcc acggcacccg
     1501 gggcacggcc ccgaggtcga tgcccgcctt gcgcagctgc gtccggatcg ggccgccgcg
     1561 ctggatcggg tggcgcagcg cctttgcgag cgcgcggttt tccagccacg agaggtacat
     1621 atagacctgc acgaaggcgc tgccggcaag gatcaccggc gcaagaagga gcgaaaggag
     1681 cacgaggatc ggctgcaggc cgcctatata catcaccgtg gcggccgcgc aggcgaggct
     1741 cgtgagcccc acgagcacgg cgatcggggc gaggagcctc gcacgctcgg tcacgtagct
     1801 gcggaagccg cgcacctcct ggtacctgcg cacgaggttc atgccctcgc tcagcgggtc
     1861 gagcagcacc gcctgcgcga tcgactgggt gatctgggcc ttgtccatgg agccagcata
     1921 caaaagcccg gccggaagtc gcgggcatat tgcgcttacg aggcgcgctc gagccgcaaa
     1981 gcgacgcgcc ggccctcgag aacctcgatt ccgcacagtc gcccgcgcgg gtcggcgtcc
     2041 acgctgacgc ccagctcgtc ataaagcgaa atgcgctgca cgtgcatcat gtccaccgga
     2101 tcggcgccga tctgcagata ggcggtgtcg gcgaactcgt cgtaggtcca gcgggaaacg
     2161 ctcgcttctt tcagctgctc gaccaggtcg ggccggccca aattgaggag ccccatttcg
     2221 aggtcggcga cgagctcggg cagcgcctgc gagagcttca tcggctcttc gaggcgtagg
     2281 tcttttccca gttcttcccg tccttcggga ccacggtcat ttgctcgacc gtgcctgcgt
     2341 ccaggcaacg cgcgttcacg ctgaagccgt ccgggtgcga gcgcggcacg tagaacgact
     2401 tgatgccgca caccgagcag aagagatgcc gcgccgtccc ggtgttgaac ttgtactcgg
     2461 cgagcacttc gctgcccgcg acgagcctga actgcgcatt gggcacgatg agatgaaggt
     2521 acccggtctt cgcacacatg gagcagttgc agtcgacgac cgagatacgc ggcggcgcga
     2581 gcacctcgaa gcgcacccgg ccgcagtgac agccgccttt atgcgtaatc atgctcagga
     2641 agatagcgct tgatcgccgc gcgcgcacgc tcgacatatt gcggtttctc gccgaccggc
     2701 gcgacgcgtg ccgatgccgc tgccgacgat taccgcctcg gccgatgaaa gagtccagtt
     2761 cgttgaagcc ttcgaatgtg aggatggcga ttcacagcgg ttatctcttc gccgccgtag
     2821 ttctcgcaaa tcctttgaac tgtgacgcgc cttagactcc ttccagcccg ggaattcgct
     2881 gatgccgcta cgcgccaagc ccatgctcgg ctgggattgg cgtcggcg
//
LOCUS       DASUNT010000244         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_296918, whole genome shotgun sequence.
ACCESSION   DASUNT010000244 DASUNT010000000
VERSION     DASUNT010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296918"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1612)
                     /locus_tag="VFX94_04860"
     CDS             complement(<1..1612)
                     /locus_tag="VFX94_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX5767549.1"
                     /translation="MACLPDYAELHCLSNFSFLRGASHPEELVSRAQALGYKALALTD
                     ECSLAGAVRAHQQAKECGLPLILGAEVVAEGLKLVLLATDRRSYGAISSLIATGRRRS
                     RKGTYCLGLSDVESLCGSGTLLLWIAGEDATPGAWIAERFRGQAWIGAELHCGPNDRA
                     RLQALREIGKSFGIPLAASGDVHMHVRSRRRLQDVLTAVRLGKPLAQCGQALFPNAER
                     HLRLRMRLAQLYPPQLLDQSVAIAERCRFSLDELRYEYPSELVPQGHTPASWLRTLTG
                     QGLRWRFPAGVPPKVADVVEHELRLIAELGYEPFFLTVHDVVRYARDQEILCQGRGSA
                     ANSVVCYALGITEVDPARMNMLFERFVSRERNEPPDIDVDFEHQRREEVIQYVYRKYS
                     RDRAALAATVICYRPKSAVRDVGKALGLPLDEVDRLAKGLIFWDTRSALPGRLEGQFL
                     ELVQTLIGFPRHLSQHVGGFVISRGPLAELVPIENAAMPDRSVIQWDKDDLEALGLLK
                     VDVLALGMLSAIRRALDMLGMKMHEVPPE"
     gene            complement(1596..>2351)
                     /locus_tag="VFX94_04865"
     CDS             complement(1596..>2351)
                     /locus_tag="VFX94_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX5767550.1"
                     /translation="DRALGTAPEPRSFFAPPPRFGAKLELPAEVTHAEGLLFGARRLL
                     LQLAGLLAARHEGVRGFRLVLLHESRASSLEIGLASATRDAERMARVLRERLAPLALA
                     EPVRALRLEAGDFTALQGASAGMFGDPAREAENWAELAERLRARLGSSAVHGLAALPE
                     HRPEHAWRCVEPGEWDPRTAPAPGVRPLWLLEPRKLQEAGFITLIGPERIESGWWDGD
                     DAKRDYFVARLPNAALAWVFREGGEWFLHGLFA"
BASE COUNT          391 a          821 c          797 g          342 t
ORIGIN      
        1 cctccggcgg gacctcgtgc atcttcatgc ccagcatgtc cagcgcacgc cggatggcgg
       61 aaagcatccc gagggccaat acatcgacct tcaaaagccc gagcgcctcg aggtcgtcct
      121 tgtcccactg gatgacgctg cgatcgggca tcgcggcgtt ctcgatcggc acgagctcgg
      181 cgagcggccc gcgcgagatg acgaagccgc cgacgtgctg cgaaagatgg cgcggaaagc
      241 cgatgagggt ctgcacgagc tccaggaact ggccctccag gcgaccgggc aatgcgctgc
      301 gggtatccca gaatatcaag cccttcgcca gacgatccac ctcgtcaagc ggcaggccga
      361 gcgccttgcc gacatcgcgc acggcgcttt tcgggcggta gcagatcacc gtcgcggcaa
      421 gcgcggcgcg gtcgcggctg tacttgcggt agacgtactg gatcacctcc tcgcgccgct
      481 ggtgctcgaa gtcgacgtcg atgtcgggcg gctcgttgcg ctcacgcgac acgaagcgct
      541 cgaacagcat gttcatgcgc gccgggtcga cctcggtgat gccgagcgcg tagcacacca
      601 ccgagtttgc cgctgagccc ctgccctggc agagaatttc ttggtctcga gcgtaacgca
      661 ccacgtcgtg cacggtaagg aagaaaggct cgtagccgag ctcggcgatg agcctgagct
      721 cgtgctccac cacgtcggcg actttcggcg gcacgcccgc cgggaagcgc caacgcaggc
      781 cctgcccggt cagcgtcctc agccacgagg ccggggtatg gccctgcggc acgagctccg
      841 acggatattc ataacgcaat tcatccagtg agaaacggca gcgctcggca atcgccacac
      901 tctggtccag cagctgcggc ggataaagct gcgcgaggcg catgcgcagc cgcaggtggc
      961 gctcggcgtt gggaaagagc gcctggccgc actgcgcgag cggcttgccc aggcgcacgg
     1021 cggtgaggac gtcctgcagc cgccggcgcg agcgcacatg catgtgtaca tcgccgctcg
     1081 ccgcgagtgg aataccgaag ctttttccaa tctccctcaa agcctgcagc cgcgcgcggt
     1141 cgttcggtcc gcaatgcaat tcggcgccga tccaggcttg gccgcgaaag cgctccgcga
     1201 tccacgcccc aggcgtggca tcttccccgg caatccacag cagcagcgtc ccgctgccgc
     1261 aaagcgattc gacgtccgaa agcccgaggc aataagttcc tttcctgctg cgtcgccttc
     1321 ccgtggcaat gagcgacgag atcgcgccat acgagcgccg gtcggtggcg agcagcacga
     1381 gcttcagccc ctcggccacg acctcggcgc cgaggatgag cggcaggccg cattccttcg
     1441 cctgctggtg cgcgcgcacc gcgccggcga gcgagcattc gtcggtcagc gcgagcgcct
     1501 tgtagccgag cgcttgcgcg cgcgaaacga gctcctcggg gtgcgaggcg ccgcgcagga
     1561 agctgaagtt ggaaaggcag tgaagctctg cgtagtcagg caaacaggcc atggagaaac
     1621 cattcgcctc cttctcggaa aacccaggcg agcgcggcgt tcggcagccg cgcgacgaag
     1681 taatcgcgct tcgcatcgtc gccgtcccac cagcccgact cgatgcgctc cgggccgatc
     1741 agggtgatga agcctgcttc ctgcagcttt ctcggctcga gaagccagag cggccgcacg
     1801 ccgggcgcgg gcgccgtgcg cggatcccat tcgcccggct cgacgcaccg ccaggcatgc
     1861 tccgggcggt gctcggggag cgcggcgagg ccgtgcacgg ccgagctgcc cagccgggcg
     1921 cgcagccgct cggcgagctc ggcccagttc tcggcttcgc gtgccggatc gccgaacatg
     1981 cctgcgcttg ccccctgcag cgccgtgaag tcgccggcct cgagccgaag cgccctgacc
     2041 ggctcggcca gcgctagcgg cgcgaggcgc tcgcgcagca cccgcgccat gcgctcggca
     2101 tcgcgcgtcg cggacgcgag gccgatttcc aggctgctcg cccggctttc gtggagcagg
     2161 acgagcctga agccgcgaac gccttcgtgg cgggcggcaa gcaggcctgc gagctgcaag
     2221 agcagccggc gggcgccgaa cagcagccct tcggcgtggg tcacttcggc aggcagctcg
     2281 agcttcgcgc cgaagcgcgg cggcggcgcg aagaagctgc gcggctcggg cgccgtgccg
     2341 agcgcccggt c
//
LOCUS       DASUNT010000245         2385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_384935, whole genome shotgun sequence.
ACCESSION   DASUNT010000245 DASUNT010000000
VERSION     DASUNT010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384935"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VFX94_04870"
     CDS             <1..470
                     /locus_tag="VFX94_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767551.1"
                     /translation="RLRSEGRFREDFFYRLCSDVIRMPTLRERIAESSAELPSLVQLL
                     VERITGSSQLVDKVLQNLPGGHAWPGNVRELEQAVRRILLTGSYAPDSVAATHADSVF
                     MEEVRAGGLTAAELLARYSAMLHRRLGTYAEVAKRTGLDPRTTRKYVESARSG"
     gene            503..1309
                     /locus_tag="VFX94_04875"
     CDS             503..1309
                     /locus_tag="VFX94_04875"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX5767552.1"
                     /translation="METLVEKARTYLDAHRGERVTLERLAGAVGASAFHLQRRFKEAF
                     GVSPRDYQDAHRVEAVKSSLKNGSRVTDALYEAGYGSVSRFYEKQRLGMSARDYRAKG
                     KGQRISSCSFASPLGTVLIAATGKGLCSVKLGDDPSRLRGLLAEEFSEAEIVQADLRE
                     LKQKILSFIQGEANLARLPLDIRGTLFQRRVWDELRRIPRGETRTYREIARALGAPDA
                     VRAVGSACGANPVALVVPCHRAVRTDGGLGGYAWGLSRKKRLLELEKKKP"
     gene            complement(1306..>2385)
                     /locus_tag="VFX94_04880"
     CDS             complement(1306..>2385)
                     /locus_tag="VFX94_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767553.1"
                     /translation="IWDSQTSTHNQMMDERGRVWAAARIRPGRNPDWCGKGSSHPSAK
                     VFPIEQAGRHLSMYDPASGKWTLISTCFPTHHLIFAEDADGTIWTSSGGAGAGVLGWL
                     KPRVLEQTGDEALAQGWTRFILDSNGNGRRDAEDRSVNIGLYSVAVSPKDGSVWGTAL
                     APFPSLVVRVAPGADPTNTALTEIYEPPFPAYGARGGDIDRNGVFWASFASGHLGAFD
                     RRKCKGPLNGPQAHGRHCPEGWALYRFPGPQFRDVQDDGSAEASYYVWVDWFNTLGLG
                     ENVPIATANMSEAYFALVDGKFVTLRVPYPQGFFAKWAEGRIDDPSAGWKGKGLWATY
                     STRAVFHSEGGTQSRPRVVKFQLRN"
BASE COUNT          371 a          814 c          822 g          378 t
ORIGIN      
        1 agcggctgcg cagcgaaggc cgcttccgcg aggacttctt ctaccgcctg tgctccgatg
       61 tgatccgcat gccgacgctg cgcgagcgca tcgccgaatc gagcgccgag ctgccctcgc
      121 tcgtgcagct gctcgtcgag cgcatcaccg ggtcttccca gctcgtcgac aaggtgctcc
      181 agaatctgcc gggcggccat gcctggccgg gaaacgtgcg cgagctcgag caggccgtgc
      241 gccgcatcct gctgaccggc agctatgcgc cggacagcgt cgcggcgacg cacgcagatt
      301 cagtcttcat ggaagaggtg cgcgcgggcg ggctcacggc agcggagctc ctcgcgcgct
      361 acagcgcgat gcttcaccgc cgcctcggca cctatgccga ggtcgccaag cgcaccgggc
      421 tcgatccacg gaccacgcgg aagtacgtgg agagcgcaag atccggatag gcgcggccac
      481 ggtttgcgcg gaaaatgcgc ctatggaaac actggtcgag aaagcgagaa cgtacctcga
      541 cgcccaccgc ggcgagcggg tgacgctgga gcgcctcgca ggcgccgttg gcgcgagcgc
      601 gttccacctg cagcgccgct tcaaggaggc cttcggggtc tcgccgcggg actaccagga
      661 cgcgcatcgc gtggaggcgg tgaagtcctc gctcaagaac ggaagccggg tgaccgatgc
      721 gctctacgag gccggatacg gctcggtgtc gcggttctac gagaagcagc gcctgggcat
      781 gagcgcccgc gactatcgcg cgaagggaaa aggtcagcgc atttcgtcgt gcagttttgc
      841 ttctcccctc ggaaccgtct tgatcgccgc cacgggcaag ggcttgtgct cggtcaagct
      901 gggggatgat ccctcgcggc tgcgagggct gctcgccgag gagttcagcg aggccgaaat
      961 cgtccaggcg gacctgcgcg agctgaagca gaagatactt tcgttcatcc agggcgaagc
     1021 gaatctcgcg cgcctgccgc tcgacatccg cggcacgctg ttccagcgcc gcgtgtggga
     1081 cgagctgcgc cgcattccgc gcggcgagac gcgcacctac cgcgagatcg cgcgcgccct
     1141 cggcgcgccg gacgcggtgc gcgccgttgg aagcgcttgc ggcgcgaacc cggtggcgct
     1201 cgtcgtgccc tgccatcgtg cggtgcgcac ggacggcggt ctcggcgggt acgcctgggg
     1261 gctctcgaga aagaagcgcc tgctcgagct ggagaagaag aagccctagt tacgcagctg
     1321 gaacttgacg acgcgcggcc ggctttgcgt gccgccctcg ctatggaaca ccgcgcgcgt
     1381 ggagtacgtg gcccaaaggc ccttgccctt ccagcccgcg gacggatcgt cgatgcgacc
     1441 ctcagcccac ttggcaaaga acccttgcgg gtagggcacg cgcagcgtga cgaactttcc
     1501 gtcgaccagc gcgaagtacg cttcgctcat gtttgcggtg gcgatcggca cgttctcccc
     1561 caggccgagg gtgttgaacc agtcgaccca gacgtagtag ctcgcttcgg cgctgccgtc
     1621 gtcctgcacg tcgcggaact gcggcccggg aaagcggtag agcgcccagc cctccgggca
     1681 atgccggccg tgcgcctgcg gcccgttcag cggccccttg cacttgcgcc ggtcgaaggc
     1741 tccgaggtga ccgctcgcga aggaggccca gaacacgccg ttgcggtcga tgtcgccgcc
     1801 gcgcgcgccg tacgccggaa agggcggctc gtagatctcc gtgagcgcgg tgttcgtcgg
     1861 atcggcgccc ggcgcgacgc gcacgacgag gctcgggaag ggcgcgagcg ccgttcccca
     1921 caccgagccg tccttcgggc tcaccgcgac gctatagagg ccgatgttca cgctccggtc
     1981 ttcggcatcg cgccggccgt tgccgttcga atcgaggatg aagcgcgtcc agccctgcgc
     2041 cagcgcctcg tcgcctgtct gctcgagcac gcgcggcttg agccagccga gcacaccggc
     2101 gcccgcgccg ccgctcgagg tccagatcgt tccgtcggcg tcctcggcga agataaggtg
     2161 gtgtgtcggg aagcaggtcg agatcagcgt ccacttcccg cttgccgggt cgtacatgga
     2221 aaggtgcctg cccgcctgct ctatcggaaa cactttcgcg gacgggtgcg acgagccctt
     2281 cccgcaccag tcggggttcc gccccggtcg aatgcgcgcg gcggcccaga cgcgcccgcg
     2341 ctcgtccatc atctggttgt gcgtgctcgt ctggctgtcc cagat
//
LOCUS       DASUNT010000246         4136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_734777, whole genome shotgun sequence.
ACCESSION   DASUNT010000246 DASUNT010000000
VERSION     DASUNT010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734777"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1076)
                     /locus_tag="VFX94_04885"
     CDS             complement(189..1076)
                     /locus_tag="VFX94_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5767554.1"
                     /translation="MADQASEAAVLFADVSGSTKLYETAGNAVAHAAVESCVNIMRQK
                     TVAAKGRVIKTIGDEVMAAFRSADDAADAAIEMQLAISQLPPVGNNTLGVRIGFNYGP
                     VVETGGDVFGDAVSLASRLAALATKGQIITARETVMMLSPILKANTRAITTIQVKGRS
                     QEVQVYELMWQQSEDMTTLASHKGVFKPRNTTLRLRMQDMEMVLSAERPSLAMGRDPS
                     ADLIIRERMASRAHGKIERRLDKFVLTDHSANGTFVTIEGDKEIVLRREEFTLRGHGW
                     IAFGQSRSGATDVVEFFCE"
     gene            complement(1229..2722)
                     /locus_tag="VFX94_04890"
     CDS             complement(1229..2722)
                     /locus_tag="VFX94_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5767555.1"
                     /translation="MSWDFLLAQFAAQFGLWWVVLPGILLGIVVGILPGFSPQNTLIM
                     LLPLTLAVPVDQAMAFMISLYCANHLAGGIPAILVKIPGSGGAAATTLDGYPMTQKGQ
                     AQQALVLCFTASVFGGLMTTLATIALLPWLSQIGLYLRSVEMVVVMLFGLTLIAVIAA
                     RDTLKALVAGFFGLLIGVIGTDHIYNTPRATFGFLELYDGVPLVPALIGLFAISEALV
                     MLEEKMIVSESGRERALQARWADTVEGLRLSLKKWWHIVWTSFVGLFIGVVPGAGASI
                     ASFVAYQQSRLWSKHPERYGTGIPEGVIAPESANNGVTSGTLVPTLALGVPGGTTAAV
                     MMIVLQYHGMVMGPRLFASNPEVAYGVFISMAVAYVFMVFTILPLARYMARVVLIETM
                     YLAPVIILVTIVAAFGERQYLFDMALALVFGVIGYIAHKTNYHVTAILIGILLGPLFE
                     QYLVRALRISQGDLTVLFSSTLGNILWAFLVLSLVLPALSNYLRKTD"
     gene            complement(2719..3186)
                     /locus_tag="VFX94_04895"
     CDS             complement(2719..3186)
                     /locus_tag="VFX94_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767556.1"
                     /translation="MKSPRGADLIIPALALGFAAYFFYSIADLAWEAKANGVVIGGVL
                     VTLIAIQVVRTALEVARGKAHLGVYPVLAPRDVLGKRIGLVAITVAFIATLHWLGLTL
                     GLFAAMVAALWVMGARGKVLAVLPLAVAAAAYLLFVALLKSDIPHGPIERLFS"
     gene            complement(3193..>4136)
                     /locus_tag="VFX94_04900"
     CDS             complement(3193..>4136)
                     /locus_tag="VFX94_04900"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5767557.1"
                     /translation="VPARGQAFPSKNMRVVIPTGQGGGADRLARVFDDFWGPLLKANF
                     EYGFFAGAAGQVGYELYLNKRERDGHNLLFGNMGPEMIMYALQKPPYRFPEDYQYFCR
                     LDVDDSIVFVRSESRIKRIEDLVDEGKKRAVNVAVSRLPHPASIGLLALGAATKARFN
                     LVPYGGGNPTTVAVMNGEADCGALPIAGVAALGDKMRILGIFNDDAQKMAKYSGNAPS
                     VNKVFGTKIPPLYSSRAWAIHTEVIEKFPDRFALLEKTARQVFENPKYREEYAKTGAP
                     IETIQYGDRKLCTEYAKGMVELTNEYRSLLSAKDKKG"
BASE COUNT          747 a         1332 c         1346 g          711 t
ORIGIN      
        1 caaatgatgt gacaaggaga gagggcctgc ggcgcagagc gaacgcgcta tccgattgtc
       61 ctgcgagcgc gccgccgctt atgtggaggc ggatctaagc gcctgctttg gcacgcaatt
      121 aagctgtgcg tgcccgcgcg cgcgcgagcg cgcccttacg tgttcttaca ttactcgcag
      181 aagaattctt actcgcagaa gaactcgacc acgtcggtcg cgccgctacg cgattgaccg
      241 aacgcgatcc agccatggcc gcgcagcgtg aactcttccc ggcgcagaac aatttccttg
      301 tcgccttcga ttgttacgaa ggtgccgttt gcgctgtggt cggtgagaac gaatttgtcg
      361 agccgccgct cgatcttgcc gtgcgcacgc gaggccatgc gctcgcggat gatcaggtcg
      421 gccgacgggt cgcggcccat ggcgagcgac ggacgctcgg cggagagcac catctccatg
      481 tcctgcattc tgagccgcag cgtggtattg cgcggcttga aaacgccctt gtgcgaggcg
      541 agcgtagtca tgtcctcgct ctgctgccac atcagctcgt acacctggac ctcctgcgac
      601 ctgcctttca cctggatggt ggtgatggcg cgcgtgttcg ccttgaggat cggcgaaagc
      661 atcatcaccg tctcgcgcgc ggtgatgatc tgtcccttgg tggcgagcgc ggcgaggcgc
      721 gaagcgaggc tgactgcatc gccgaataca tcgccgccgg tctcgaccac cgggccgtag
      781 ttaaagccga tacggactcc gagcgtgtta tttcccaccg ggggaagctg cgaaatggcg
      841 agctgcatct cgatcgcggc gtcggctgcg tcgtcggcgc tgcggaaggc ggccatcact
      901 tcgtcgccga tagtcttgat cacccggccc ttcgccgcca cggtcttctg gcgcatgatg
      961 ttcacgcagc tttcgaccgc cgcgtgcgcg accgcgttgc cggccgtctc atacagcttg
     1021 gtgctgccgc tgacgtcggc aaagagaacc gctgcctcgc tcgcctggtc ggccatctag
     1081 acgtgcatcc ctccccgttg gtcgattgta cctgccccga aagaagattc agggggtgac
     1141 cgtttcgttt tttcgtcggt cttcagccgt gcgctctgga tgtgcgcccg ggtttcgcgc
     1201 aattcgaagc ggcaagccgc gcgaggagct aatccgtttt ccggagatag ttgctgagcg
     1261 ccggcaggac gagcgaaagg acgagaaacg cccagaggat attgccgagc gtcgaggaga
     1321 acagcaccgt gaggtcgccc tgcgagatgc gcagcgcgcg caccaggtac tgctcgaaga
     1381 gcggtccgag caggatgccg atcaggatcg cggtgacgtg gtagttggtc ttatgcgcga
     1441 tgtaaccgat cacgccgaat accagcgcaa gcgccatatc gaacagatat tggcgctcgc
     1501 cgaaggctgc aacgatggtg acgaggatga tgaccggggc aaggtacatc gtctcgatga
     1561 gcacgacgcg cgccatgtag cgcgcgagcg ggaggatggt gaacaccatg aagacgtacg
     1621 cgaccgccat ggagatgaaa accccgtagg caacctcggg gttcgaggcg aaaagccgcg
     1681 ggcccatcac catgccgtgg tactgcagca cgatcatcat caccgccgcg gtggtcccgc
     1741 ccgggacgcc gagcgcgagc gtcggcacca gcgtgccgga ggtcacgccg ttattcgccg
     1801 attccggcgc aatcacgcct tccggaatcc cggtcccgta acgctcggga tgcttcgacc
     1861 agagccgcga ctgctggtac gcaacgaacg atgcgatgct tgccccggcg cccggcacga
     1921 ccccgatgaa gagcccgacg aacgatgtcc agacgatgtg ccaccatttc ttcagcgaga
     1981 ggcgcaggcc ttcgactgtg tcggcccaac gagcctgcaa cgcgcgttcg cggccgctct
     2041 cgctgacgat catcttctcc tcgagcatca ccagcgcctc tgaaattgcg aacaagccga
     2101 taagcgccgg cacgagcggc acgccgtcgt agagctcgag aaagccgaac gtggcgcgcg
     2161 gcgtgttgta gatgtggtcg gtgccgatca ctccgatcag cagcccgaag aacccggcga
     2221 ccagcgcctt cagcgtgtcc cgggccgcga tcacggcgat gagcgtcagg ccgaacagca
     2281 tcaccaccac catttccacc gagcgcaggt acaggccgat ctgggacagc cagggcagca
     2341 gcgcaatcgt ggcgagcgtc gtcatcaggc cgccgaatac ggaggccgtg aagcagagca
     2401 cgagcgcctg ctgcgcctgc cctttctgcg tcatcgggta gccgtcgagg gtcgtagccg
     2461 cggcgccgcc cgagcccggg atcttcacca ggatggccgg aatcccgccc gccaggtggt
     2521 tcgcgcagta gagcgagatc atgaacgcca tcgcctgatc gaccggtacg gcgagcgtaa
     2581 gcgggagcag catgatgagc gtgttctgcg ggctgaagcc aggcaggatc ccgaccacga
     2641 tgccgagcag gatgccgggc aggacgaccc accagagccc gaactgcgca gcgaactgcg
     2701 ccaggagaaa gtcccagctc aagagaagag tctctcgatg ggcccgtgcg gaatgtccga
     2761 cttcagcagc gccacgaaga gcaggtaggc ggcggcggct acggcgagcg gcaggacggc
     2821 gagcaccttg ccgcgcgcgc ccataaccca cagcgccgcg accatcgcgg cgaacagtcc
     2881 aagcgtcagg ccgagccagt gcagcgtcgc tataaaggcg accgtgatgg cgactagccc
     2941 gatgcgctta cccagcacgt cgcgcggcgc gagcaccggg tacaccccga ggtgcgcctt
     3001 gccgcgcgcc acctcgagcg cggtgcgcac gacctggatg gcgatcagcg tgaccagcac
     3061 gccgccgatc accacgccgt tcgccttcgc ctcccaggcg aggtcggcga tcgagtagaa
     3121 gaagtaggcc gcgaacccga gcgcgagcgc cgggatgatc aggtccgcgc cgcgcggcga
     3181 cttcaccccc gcctagccct tcttgtcctt ggcgctcagg agggagcggt attcgttcgt
     3241 aagctcgacc atcccttttg cgtactcggt gcagagcttg cgatcgccgt actggatggt
     3301 ttcgatcggg gccccggtct tcgcatactc ctcgcggtat ttcgggtttt cgaacacctg
     3361 gcgcgccgtc ttctcgagca acgcgaagcg atccgggaac ttctcgatga cttccgtatg
     3421 gattgcccag gcgcgagagg agtagagcgg cgggatcttg gtgccgaata ccttgttcac
     3481 cgacggcgcg ttgccgctgt acttggccat cttctgcgcg tcgtcgttga agatcccgag
     3541 gatgcgcatc ttgtcgccga gcgcggcaac gcccgcgatc ggcagcgcgc cgcagtcggc
     3601 ctcgccgttc attaccgcca ccgtggtggg gttgccgccg ccgtagggaa cgaggttgaa
     3661 gcgcgccttg gtcgccgcgc cgagtgcgag caggccgatg gaggcggggt gcggcaggcg
     3721 gctcaccgcc acgttgaccg cgcgtttctt gccctcgtca accaggtcct cgatgcgctt
     3781 gatgcggctt tcgcttctca cgaacacgat gctgtcgtcc acgtcgaggc ggcagaagta
     3841 ctggtaatcc tcggggaagc ggtacggtgg cttctgcagc gcgtacatga tcatctccgg
     3901 ccccatgttg ccgaagagca ggttgtgccc gtcgcgctcg cgcttgttca ggtagagctc
     3961 atagcccacc tggcccgccg cgccggcgaa gaagccgtat tcgaaattgg ccttgagcag
     4021 cgggccccag aagtcgtcga acacgcgcgc caggcgatcg gcgccgccgc cctggccggt
     4081 cggaatcacc acccgcatgt tcttcgacgg gaacgcctgc ccgcgggcgg gcacgg
//
LOCUS       DASUNT010000247         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_385009, whole genome shotgun sequence.
ACCESSION   DASUNT010000247 DASUNT010000000
VERSION     DASUNT010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385009"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1622
                     /gene="glnE"
                     /locus_tag="VFX94_04905"
     CDS             <1..1622
                     /gene="glnE"
                     /locus_tag="VFX94_04905"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEX5767558.1"
                     /translation="LVPESAARELSEAYRFLRSLEHRLQYLDDAQRHDLPQDAEDRTR
                     VAAMCGFASWDAFYARWRAMRDSVSRHFVDVFAEPSAAPEPWPQHPRLAALRASQRYA
                     ALPEQSRRRLERLVPALARAAQATPDPEVTLARGVDLVEAIASRAAYLALLAEHPQAL
                     ERVARMIGASSWAAEYVTRHPLLLDELLDDRLLYAPPDWVAFSQSLKQQLAVAREDTE
                     RRMNILREMHQAQVFRLLAQDLAGLLTVETLADHLSALADLVLQACIDEVWRDLRGRH
                     RDPPSFAVIGYGKLGGKELGYVSDLDIIFLYDDDDERAPEVYARLAQRLNTWLSSRTS
                     SGMLFETDLELRPSGASGLLVSSIAAFARYQDESAWVWEHQALSRARYCAGARAVGEK
                     FEAIRQRILTRRRDPEPLKAEIVAMRDRLHAAHPNASALFDVKHDAGGMIDIEFAVQF
                     LVLAFSSRFPSLTGNLGNIALLRMAAGHGLIRAELAERARDAYREFRRLQHRLRLNGA
                     QYARVPREQVSAEAEAVRTLWREVFGRASGP"
     gene            complement(1709..2026)
                     /locus_tag="VFX94_04910"
                     /pseudo
     CDS             complement(1709..2026)
                     /locus_tag="VFX94_04910"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
BASE COUNT          300 a          717 c          722 g          331 t
ORIGIN      
        1 ctctcgtgcc ggaaagcgcg gcgcgcgagc tgagcgaggc ctaccgtttc ctgcgaagcc
       61 tcgagcaccg gctgcagtac ctcgacgacg cgcagcgcca cgacctgccg caggacgccg
      121 aggaccgcac gcgcgtcgcg gcgatgtgcg ggttcgcctc ctgggacgcg ttctacgccc
      181 gctggcgggc gatgcgcgac tcggtgtcgc gccatttcgt cgacgtgttc gccgagccga
      241 gcgcagcgcc cgagccctgg ccgcagcatc cgcggctcgc cgcgttgcgc gcgagtcagc
      301 gttacgccgc gctgccggag caatcgcgcc gccgcctcga gcgcctggtt cccgcgctcg
      361 cgcgcgcggc ccaggcaacg ccggacccgg aggttacgct cgcgcgtggc gtcgatctgg
      421 tcgaggcgat cgcgagccgc gccgcgtacc tcgctttgct cgccgagcac ccgcaagcgc
      481 tcgagcgcgt ggcgcgcatg atcggcgctt cgagctgggc tgccgagtac gtcacgcgcc
      541 atccgctgct gctcgacgag ctgctcgacg accggctgct gtacgcgccg ccggactggg
      601 tcgcgttctc gcagagcctg aagcagcagc tggccgtcgc ccgggaagac accgagcggc
      661 gcatgaacat cctgcgcgag atgcaccagg cacaggtgtt ccgattgctc gcgcaggatc
      721 tcgccggact gctcacggtg gagacgctcg ccgatcacct ctcggcgctc gccgacctgg
      781 tgctgcaggc gtgcatcgac gaggtgtggc gcgacctgcg cggccggcac cgcgacccgc
      841 caagtttcgc cgtcatcggc tacggcaagc tcggcggcaa ggagctcggc tatgtctccg
      901 acctggacat catcttcctc tatgacgacg acgacgagcg cgcgcccgag gtctatgcgc
      961 gcctcgcgca gcggctcaac acctggctga gcagccgcac ctcgtccggg atgctgttcg
     1021 agaccgacct cgagctcagg ccgagcgggg cctcggggct gctcgtttct tcgatcgccg
     1081 ctttcgcgcg ctaccaggac gagagcgcct gggtgtggga gcaccaggcc ctttcccgcg
     1141 cgcgctactg cgcaggcgcc cgcgccgtgg gcgaaaagtt cgaggccata cggcaacgca
     1201 ttctcacgcg caggcgcgac cccgagccgc tcaaggcgga aatcgtcgcg atgagggaca
     1261 ggctgcacgc ggcgcacccg aatgcgtcgg cgctgttcga tgtcaagcac gatgcgggcg
     1321 gcatgataga catcgagttc gcggtgcagt ttctggttct cgccttttcc agccgctttc
     1381 cctcgctcac cggcaatctc gggaacatcg cgctgctccg gatggcggcg gggcacggcc
     1441 tgattcgggc cgagctcgcc gagcgcgcgc gcgacgcgta ccgcgaattt cgccggctcc
     1501 agcaccgcct gcgcctgaac ggcgcgcagt acgcgcgcgt gccgcgcgag caggtctcgg
     1561 ccgaagccga ggccgtcagg acgctgtggc gggaggtctt tggccgagcc tcaggtcctt
     1621 gatctccggg gagtcggtgt cgagctcgcc gcgctcgagc ttgcgccgcg cggccgccgc
     1681 gagcttccgc gtagctcctg cgatagcgct cgaacttctc gtcgtcgata tgctcgaggt
     1741 cgagcagcgc gttgtgcgcg ccctgggtag cgcggatgag ctcgtcgagc ttgagctgga
     1801 tcgcctcggt atcgcggttc tgggtgctct ggatgacgaa taccatgagg aacgtgatga
     1861 tcgtcgtcgc ggtgttcacg acgagctgcc agctgtcgct gtagccgaaa agcggcccgg
     1921 tgagcgccca gagcaggagc gcggccaccg cggcgaagaa agtggccggg cgcccggcca
     1981 gcagcgcgag cctgcgcgcg aggtacgaga acctgaattt gttcattgcc cgctccgaaa
     2041 ggtaatgtcg ccgtagaacg cgcggcgggt
//
LOCUS       DASUNT010000248         2859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_735134, whole genome shotgun sequence.
ACCESSION   DASUNT010000248 DASUNT010000000
VERSION     DASUNT010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735134"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VFX94_04915"
     CDS             <1..661
                     /locus_tag="VFX94_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty-acid--CoA ligase"
                     /protein_id="HEX5767559.1"
                     /translation="GPQTMRAIIDRMGAKAICAAYGLSEASPNVVMSDWHDPEELRVQ
                     GLARPHDGVELRISPEGEIQVRGWNVMRGYYNNPEANAKAFTADGWLRTGDLGELTSD
                     GRLRMSGRLKDVFRVGGENVAPAEVEEVLLAHPAVETAQVIGVPDARLGEVACAYVTL
                     KSRASEEELLDWCKKRCANFRVPRYLKIVESFEAIGMTASGKVQKTKLREHALRELKL
                     G"
     gene            658..2133
                     /locus_tag="VFX94_04920"
     CDS             658..2133
                     /locus_tag="VFX94_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5767560.1"
                     /translation="MKLRGADVVARTLAAAGVRQLFALSGNHVMPLFDAALDTGLRLI
                     HVRHEAAAVHMADAWGRLTGEVGVALVTGGPGHANAVSALYTALEAESPVLLISGHAA
                     LAELGKGAFQEMRQAELAAPAAKASWTVQSADRLAHDLGRALREARSGRPGPVHVSIP
                     QDVLEAATDFDIPEIPATAEQASKPMPAELLEAKRPLVIAGPAMMRHPRLRALEQASG
                     VPVVGMESPRGVNDPSLGRLAEILKRADAALLLGKKLDFTLRFGAAFAAGCRLLHADT
                     IPDPFGDAALTPRSWVEEVQAGLAYRPPEWKAIDATLHPVVVCRSVQKLLDAPEAVLV
                     CDGGEFGQWAQACLSAPRRIINGPAGSIGSALPFAAAAKLAFPQAPVVAMLGDGTFGF
                     HMSELDTAVRYGLHYIAVIGNDACWNAEYQIALKSYGSGRAKNLDLLPTRYGAATAAL
                     GGHGEDVTQFEQLASALERARKAAKPACVNVLIERQPAPRY"
     gene            complement(2134..>2859)
                     /locus_tag="VFX94_04925"
     CDS             complement(2134..>2859)
                     /locus_tag="VFX94_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767561.1"
                     /translation="TVAPHMPANAPAWLTDEELAVYAQAFQRTGFQGGLNWYRAATNP
                     APAPKIERNTIDIPSIYIAGAADWGIYQKPGELERMQRDVCTDFRGVHLVEGAGHWLQ
                     QEQPERVAALLLDFLRTIPPRAAHPSRSPDHDASSVAGPRCCRSARRGNPSPGATEAG
                     FPDMNKIVGWTALMGPPGMSKDVVEKWTEVFAKLAADPDWQQGNARIGGIAAIRPPAE
                     TERFVREQYELYDKLVGTLGIRQ"
BASE COUNT          469 a          909 c         1018 g          463 t
ORIGIN      
        1 cggcccgcag accatgcgcg cgatcatcga caggatgggg gcgaaagcga tctgcgcggc
       61 gtacgggctg tcggaggctt cgcccaacgt ggtgatgagc gactggcacg acccggaaga
      121 gctgagggtg cagggcctcg ccaggccgca cgacggggtg gaactgcgga tttccccgga
      181 aggagagatc caggtgcgcg gctggaacgt gatgcgcggc tattacaaca acccggaagc
      241 gaacgcgaag gcatttactg cggacggttg gctgcgcact ggagacctcg gcgagctgac
      301 gtccgacgga aggttgcgca tgtccggccg cctcaaagac gtgttccgtg tcggcggaga
      361 aaacgttgcg ccggccgagg tcgaagaggt tctgctcgcg catccggcgg tcgagaccgc
      421 gcaggtgatc ggcgttcccg atgcgcggct cggcgaggtc gcgtgcgctt atgtcacgtt
      481 gaaatcgagg gcgagcgaag aagagctgct cgactggtgc aagaaacgct gcgcaaattt
      541 ccgcgtgcca cgatatctga agatcgtgga aagcttcgag gctatcggca tgacggcgag
      601 cggcaaggtg cagaagacca agctgcgcga gcatgcgttg cgcgagctga agctggggtg
      661 aagctgcgcg gcgcagacgt cgtggcgcga acgctcgccg ccgccggcgt gcgccagctg
      721 tttgccctgt ccggaaacca cgtgatgccg ctcttcgatg ccgcgctgga cacgggcctg
      781 cgcctcatcc acgtgcgaca cgaggcggcc gcggtccaca tggcggacgc ctggggccgc
      841 ctgaccgggg aagtgggcgt cgcgctcgtc accggcgggc cgggccacgc gaacgccgta
      901 tcggcgctct acacggcgct cgaggccgag tcgcccgtat tgctgatctc tggccacgcc
      961 gcgctcgcgg agctcggcaa gggcgcgttc caggaaatgc gccaggccga gctcgccgcg
     1021 ccggctgcga aggcttcgtg gaccgtgcag tcggccgatc gccttgcgca cgacctcggg
     1081 cgcgccctgc gcgaggcgcg ctccgggaga ccgggcccgg tgcacgtcag cattccgcag
     1141 gatgtgctcg aagcggcgac ggacttcgat attccggaaa ttcccgccac ggctgaacaa
     1201 gcttcgaagc cgatgccggc cgagctgctg gaagcgaagc ggccgctggt catcgccggg
     1261 ccggccatga tgcggcatcc aaggttgcgc gcactggagc aggcgagcgg cgtgccggtg
     1321 gtcggcatgg agagcccgcg cggcgtgaac gatccatcgc tcgggcggct cgccgaaatt
     1381 ttgaagcgcg ccgatgcggc gctgctcttg ggcaagaagc tcgacttcac gctcaggttc
     1441 ggcgccgctt tcgcggccgg ctgccgcctg ctgcacgccg ataccattcc tgacccattc
     1501 ggggacgcag ccctgactcc tcgaagctgg gtggaagaag tccaagccgg gctcgcttat
     1561 cgcccgcccg agtggaaggc gatcgatgca acgctgcatc ccgtggtcgt ctgccgttca
     1621 gtgcagaagc tcctcgacgc gccagaggcg gtgctggttt gcgacggcgg cgagttcggg
     1681 caatgggcgc aggcgtgcct gtccgctccc aggcgcatca tcaacgggcc cgcgggctcg
     1741 ataggctcgg cgctgccgtt cgccgcggcg gccaagctcg ctttccctca ggccccggtg
     1801 gtggcaatgc tgggcgacgg aacgttcggg ttccacatgt cggagctcga cacggcggtc
     1861 cgctacggcc tccattacat cgcggtcatc ggcaacgacg cctgctggaa cgccgagtac
     1921 cagatcgcgc tcaagtcgta cggcagcggc cgtgccaaga atctcgatct attgccgaca
     1981 cgctacggcg cggcaaccgc ggcgctgggc ggacacggag aggacgtcac ccagttcgag
     2041 cagctcgctt ccgcgctcga gcgcgcaaga aaggcggcca agccggcctg cgtcaatgtg
     2101 ctgatcgagc gccagccggc gccgcgctat tagctattgc cgaatgccga gcgtaccgac
     2161 gagcttgtcg tagagctcgt actgctcgcg cacgaagcgc tcggtctccg cgggcgggcg
     2221 gatcgcggcg atgccgccga tgcgcgcgtt gccctgctgc caatccgggt cggcggcaag
     2281 cttggcgaag acctcggtcc acttctcaac gacgtccttc gacatcccgg gcggtcccat
     2341 gagcgcggtc cagccgacga tcttgttcat gtcgggaaag cccgcttccg ttgcacctgg
     2401 cgagggattt ccacgcctcg cgctacggca gcatcgtggc ccggcgaccg aggaggcatc
     2461 gtggtcaggg gaacgacttg ggtgtgccgc tcgaggcgga atagtcctca ggaagtcgag
     2521 cagcaacgca gccactcgtt ccggctgctc ctgctgcagc cagtggccgg caccttccac
     2581 gaggtggacg ccacggaagt cggtgcagac gtcgcgctgc atgcgctcga gttcgccggg
     2641 tttctggtag atgccccagt cggcggcgcc ggcgatgtag atcgaaggaa tgtcgatcgt
     2701 gttgcgctcg atttttggcg cgggggcagg attggttgcc gcgcggtacc agttcaggcc
     2761 gccctggaag ccggttcgct ggaaggcctg cgcatagacg gcgagctctt catcggtgag
     2821 ccacgcaggg gcgttcgccg gcatatgggg cgcgacggt
//
LOCUS       DASUNT010000249         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_560032, whole genome shotgun sequence.
ACCESSION   DASUNT010000249 DASUNT010000000
VERSION     DASUNT010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560032"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1279
                     /locus_tag="VFX94_04930"
                     /pseudo
     CDS             14..1279
                     /locus_tag="VFX94_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(1321..2208)
                     /gene="hemH"
                     /locus_tag="VFX94_04935"
     CDS             complement(1321..2208)
                     /gene="hemH"
                     /locus_tag="VFX94_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX5767562.1"
                     /translation="MRDYLAEFLSDPRVVKLPRWLWQPVLHGIVLRTRPEKSAEKYSQ
                     IWTPEGSPLAVYTARQSQLLQKQLGIPVRHAMRYGQPSVPSAFSALAEPVVIPLYPQY
                     SESTTASVADVLPPGVPMLEHFHDHPAYIAALAANLRRHWELYGRGAKLVMSFHGLPK
                     NGSARYERECRATARLLAERLETEHLVTFQSRFGYAEWLQPYTEPTLVELARQGVRRV
                     DVVCPGFVSDCLETLEEIGITARRAFLAAGGREFYLASCLNDSVEWIEALAALAGAIG
                     NTRASASPRDCAGSGRLKR"
     gene            complement(2262..>2392)
                     /locus_tag="VFX94_04940"
     CDS             complement(2262..>2392)
                     /locus_tag="VFX94_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003271934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX5767563.1"
                     /translation="YSVDGEVVGTVGVIGPTRMAYDRVIPIVDVTAKLLSSALSQH"
BASE COUNT          370 a          808 c          849 g          365 t
ORIGIN      
        1 ggtgtacgcg ctcatgcagc gcgttctctc gcgctacgag cgcctgctgc gcgagaagga
       61 gcgcgagcgc gcggcgcagg aagagcgcat ccgccaccag gcctatcacg acgcgctcac
      121 cgggcttccc aaccgggcga gcttcaccga gcacctggac gaggcgacgc gacgcgcgaa
      181 gcgcgccggc tggccgctcg cgctgctctt tctcgacctc gacctcttca agcgcgtgaa
      241 cgacagcctc gggcacgacg ccggcgaccg gctgctgcgc atcgccgccg agcgcatcgg
      301 gcgggcggtg cgcgaggccg acatgctgtt ccgcatgggc ggcgacgaat tcaccgtgct
      361 gctcgaggac gtgcgcggac ccgaggaggc ggcgctggtg gcgggccggg tgctcgagtc
      421 catggccgag ccgctttcgc tcgggcacca cgagatcgcg gtcaccgcga gcatcggcat
      481 cgcgctctac ccgcgcgacg acacgctcgg cgagcgcctg gtgaagagcg ccgacaccgc
      541 gatgtaccgc gccaaggagc tcggccgcaa ccgctacgcg ttcttcgcgc gcgagatgaa
      601 cgagcgcgtc gagagccaga tgatgctcga ggcggcgctg cggcgcgcgc tgaagaacgg
      661 ggaattcgtg ctgcacttcc agccgcgcgt gtcggcgctc accggccgcg ccaccggcgc
      721 ggaggcgctg ctgcgctggc gccacccgga atgggggctg gtcgagccgg cgcgcttcgt
      781 gccgctgctc gaggaaaccg gcctgatcgt gccggtcggc gcctgggtgc tcggcgaggc
      841 ctgtcgccag gcgcgtgcgt ggcagtcggc gggcctcggg ccgctgcggg tgtcggtcaa
      901 cctgtcatcg cgccagttcc gcagcgacgc gttcgccgag gcggtgagcg aggcgctgcg
      961 cggctccggc ctcgcgcccg cgctcctcga gatcgagctg accgagagcc tgctcgtgga
     1021 gaacgtcgag cacgcgatgg gcgtgatggg aaagctgaaa gcgatcggcg ccgcgctctc
     1081 gatcgacgac ttcggcaccg gctattcctc gctcgggtac ctcaagcgct tcccgatcga
     1141 cagcctgaag atcgacaaga gcttcgtgcg cgacatcgcg acgagcccga aggacgcggc
     1201 catcgtcagg gcgatctcgg cgctcgcgcg ctccctcggc atcgggctca tcgccgaggg
     1261 cgtggaggag ccctggcagg tcgaattcct gcgccagcgg cactgcaccg agatgcaggg
     1321 ctaccttttc agccgcccgc tgccggcgca gtctctcggc gaagcgctcg cgcgggtgtt
     1381 cccgatggcg ccggccagcg cggcgagggc ctcgatccat tccaccgagt cgttgaggca
     1441 gctcgcaagg tagaactcgc gcccgcccgc ggcgaggaag gcgcgccgcg cggtgatgcc
     1501 gatctcttcc agcgtctcga ggcagtccga aacgaatccc ggacagacca cgtccacgcg
     1561 gcgcacccct tgccttgcaa gctcgaccag cgtcggctcc gtgtagggct gtagccactc
     1621 ggcataaccg aagcgcgatt ggaacgtgac cagatgctcg gtttccagac gctcggcgag
     1681 aagccgcgcg gttgcgcggc attcgcgctc gtagcgcgcc gatccgttct tcggcaaccc
     1741 gtggaagctc atcaccagct tcgcgccgcg cccgtacagc tcccagtgcc ggcgtaggtt
     1801 ggccgccagc gcggcgatgt atgcgggatg atcgtggaaa tgctcgagca tcggtacgcc
     1861 cggcggcagt acatcggcca cgcttgcggt ggtgctttcc gagtattgcg ggtaaagagg
     1921 aatcaccacc ggctccgcaa gcgcgctaaa agccgatggc accgagggtt ggccgtagcg
     1981 catcgcgtgc ctgacgggaa tgccgagctg cttctggaga agttgcgact ggcgcgccgt
     2041 gtagacggcg agcggcgagc cttcgggcgt ccagatctgg gagtactttt cagccgactt
     2101 ctcgggtcga gtcctgagga cgatgccgtg cagtaccggt tgccacagcc agcgcggcag
     2161 ctttaccacg cgtggatcgg acaggaactc ggcgaggtag tcgcgcaccg cctgcggcgt
     2221 cggcgcggcg ggcgttccca ggttgacgag gagaacgctc ttcagtgctg ggagagcgcg
     2281 ctcgacagga gcttcgcggt cacgtcgacg atcgggatga cgcggtcgta ggccatgcgc
     2341 gtgggcccga tcacgcctac cgtgccgacc acttcgccat ccacgctgta gg
//
LOCUS       DASUNT010000250         2367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_998390, whole genome shotgun sequence.
ACCESSION   DASUNT010000250 DASUNT010000000
VERSION     DASUNT010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998390"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFX94_04945"
     CDS             <1..228
                     /locus_tag="VFX94_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767564.1"
                     /translation="DTLRAPLALAPDTVANAIETLERDWFVPLLAALRGARIGMLTLH
                     VPDAAEALSFEIIRRDLRRFWRLRQPIKNYT"
     gene            225..1910
                     /gene="recJ"
                     /locus_tag="VFX94_04950"
     CDS             225..1910
                     /gene="recJ"
                     /locus_tag="VFX94_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX5767565.1"
                     /translation="MKIVERPYVEASRRALLEAGMHPVLARVYAGRNIGSAAELEYQL
                     GSLLPPALLKGIDVAASLLADAIAQSKRLLIIADYDADGATACAVGMRALKAFGASVE
                     YLVPDRFKLGYGLSPELVELAATRKPDLLVTVDNGIASVEGVARARSLGIATLITDHH
                     LPGGELPAAECIVNPNQPGCPFPSKALAGVGVMFYVMLALRAELRRRGAFEQRQEPNL
                     AAVTDLVALGTIADVVPLDANNRNLVAQGLRRLRAGRATPGIAALLRAAGRPLSEASS
                     FDLGFVAGPRLNAAGRLADMSLGIECLVTDDEARAANCAQELDRLNRERRGIEASMLQ
                     QALHALDKVDSSESGVFFHPDWHQGVVGIVASRLKDRLHRPVICFARAEEAGAIVLRG
                     SGRSIPGLHLRDALDAVSKRTPGLIRRFGGHAQAAGLSIAPSDYERFAGEFSRTAQEL
                     LPAQARLRVVETDGALDAMHFSLDVARLLDAGIWGQGFPQPLFCDTFAVENQRVVGER
                     HLKLWLVKGGRRIEAMRYGALDPLPAQVRAAYRLSVNEFNGLKNIQLNVEHFE"
     gene            1907..>2367
                     /locus_tag="VFX94_04955"
     CDS             1907..>2367
                     /locus_tag="VFX94_04955"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5767566.1"
                     /translation="MKLLLSFLFAGFTVAQAADLQPIKDAGPYVPSPQSVVSDMLRYA
                     DVGPDDFLIDLGSGDGRIVLTAAKVFGARGFGVEIKEDLVRLSNESAQKEGVADRVKF
                     MKQDLFKTDMSLATVITMYLLPDTVNLLKDKFLNELRPGTRIVSHDYPLTG"
BASE COUNT          380 a          810 c          781 g          396 t
ORIGIN      
        1 gacacgctgc gcgccccgct cgcgctcgcg cccgatacgg ttgcgaacgc gattgaaacg
       61 ctcgagcgcg actggttcgt gccgcttctg gccgctttgc gcggcgcgcg catcggaatg
      121 ctgacgctgc atgtgcccga cgccgccgaa gcgctaagct tcgaaatcat acgccgcgac
      181 ctgcggcgct tctggcgcct gcggcagccg atcaagaact acacatgaaa atcgtcgagc
      241 gcccgtacgt ggaagcgagc cgccgcgcgc tgctcgaggc ggggatgcat cccgtgctcg
      301 cccgcgtcta cgccggccgg aacatcggct ccgcggccga gctcgaatac cagctcggat
      361 cattgctgcc gcccgctctc ttgaagggga tcgatgtggc tgcgagcctg cttgccgacg
      421 ccatcgcgca atcgaagcgt ttgctcatca tcgcggacta cgatgccgac ggcgcgaccg
      481 cgtgcgcggt aggcatgcgg gcgctgaaag cgtttggcgc gagcgtcgag tacctggttc
      541 ccgaccgctt caagctcggg tacggacttt cccccgagct cgtcgagctt gcggcgacga
      601 ggaagcccga tctgctggtc accgtggaca acggaatcgc aagcgtcgag ggcgtagcgc
      661 gcgcccgcag cctcggcatc gcgacgctca tcaccgacca tcaccttccc ggcggggagc
      721 tccccgcggc cgagtgcatt gtcaacccca accagccggg ctgcccattt ccgtccaagg
      781 cgctcgccgg cgttggagtg atgttctatg tgatgctcgc cctgcgggcg gaactcagaa
      841 gacgaggcgc ctttgagcaa aggcaagaac ccaacctcgc ggcagtgacg gaccttgtgg
      901 cgctcggcac gatagccgac gtggtgccgc tcgatgcgaa caaccgaaat ctcgttgcgc
      961 agggcttgcg gcgcctgcgc gccggacgcg ccacgcccgg catcgcggcg ctgctgcgcg
     1021 ccgccggccg gccgctctcc gaggcttcga gcttcgacct cggcttcgtc gcgggcccgc
     1081 gcctgaatgc cgccgggcgc cttgccgaca tgagcctcgg catcgagtgc ctggtcaccg
     1141 atgacgaagc gcgcgctgcg aattgcgcgc aggagctgga caggctcaac cgcgagcgcc
     1201 gcggcatcga ggcttcgatg ctgcaacagg cattgcacgc gctcgacaag gtcgattctt
     1261 cggaaagcgg cgttttcttc catcccgact ggcaccaggg cgtggtcggg atcgtcgcct
     1321 cgcgcctcaa ggaccggctg caccgcccgg tgatctgctt cgcgcgggcg gaggaggcgg
     1381 gcgccatcgt attgcgcggc tcggggcgct cgatccccgg actgcacctg cgcgacgcgc
     1441 tcgacgcggt gtcgaagcgc acaccggggc tcatccgccg cttcggcggc catgcgcagg
     1501 ccgcagggct cagcatcgcg ccatccgact atgagcgctt cgccggcgag ttctcgcgca
     1561 cggcgcaaga gctgctgccg gcccaggcgc ggctgcgcgt ggtggagacc gacggcgcgc
     1621 tcgacgcgat gcatttttca ctcgacgtgg cgcgcctgct cgacgcgggc atctggggcc
     1681 agggctttcc gcagccgctc ttctgcgata cattcgccgt ggaaaaccag cgcgtcgtgg
     1741 gcgagcggca cttgaagctc tggctggtga aaggcggccg ccgcatcgag gcgatgcgct
     1801 acggcgctct ggatccgctc ccggcccagg tccgggcggc ctaccggctg agcgtcaacg
     1861 agttcaacgg cctgaagaat atccagctca acgtggagca cttcgaatga aactccttct
     1921 gtcctttctc tttgcggggt tcaccgtggc ccaggccgca gacctgcaac ccatcaagga
     1981 cgcgggtcct tatgtgcctt cgccgcaaag cgtggtgtcc gacatgctgc gctacgcgga
     2041 cgtcgggccg gacgacttcc tcatcgacct cggctcgggc gacggccgca tcgtgctcac
     2101 cgcggccaag gtgttcggcg cgcgcggctt cggcgtggag atcaaggaag acctggtgcg
     2161 tctctcgaac gagagcgcgc agaaggaagg cgtcgccgac cgcgtcaagt tcatgaagca
     2221 ggatctcttc aagaccgaca tgtcgctggc gaccgtcatc acgatgtacc tgctgccgga
     2281 cacggtgaac ctgctcaagg acaagttcct gaacgagctg cgtcccggca cacgtatcgt
     2341 ttcgcacgac tacccgctca cgggctg
//
LOCUS       DASUNT010000251         2980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_34095, whole genome shotgun sequence.
ACCESSION   DASUNT010000251 DASUNT010000000
VERSION     DASUNT010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34095"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VFX94_04960"
     CDS             complement(<1..977)
                     /locus_tag="VFX94_04960"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX5767567.1"
                     /translation="MPAISSSFHNLGRYAAIAMGVSIPISTALDNVLFALILIAWLAS
                     GHLREKLASIRQNPAALAALALLGLLLLGMAWGPGSARDGWYYMNKYKELLLVAVLGA
                     FVLRERDKHLALNAFLAAMALTFVVSSAIWLGLVPVDGHFGRDVGNPTAFKLHITHGV
                     LMALASFIAADRALRAARSGWRIAYAVAAVLAASNVFMVQGRTGHLVCALLWLYLCAA
                     HWRWRGVAAAAFAGVLGLAVAYMADAPLIGRSTFALAELRAWNAGRVNPESSTGVRMT
                     YYKTTAAIIRDHPVIGVGTGGFITAYREKIRGTGLPEANNPHNQYLLTAA"
     gene            complement(958..2049)
                     /locus_tag="VFX94_04965"
     CDS             complement(958..2049)
                     /locus_tag="VFX94_04965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5767568.1"
                     /translation="MPKLARVLFTESSPNIGGQELQLLEQARGLAGRGVQTRLLCPET
                     SRIAELAVRYRVPVTAVPFRNSVHPRSVLGVRRVLADWRPDAVLCHSGHDADVSALAA
                     RLVGRPRPRLVRMRTYQHGSPHAWTYNRLFDRTLVPSEEMRARLLANPRVGAERIAVL
                     RPGIAFAELDARAALPLDAALEQALSRFPPRRLTHAAMLRGEKGHLFMLEVLARLRKS
                     VPELCYVIAGEGELKERIDQQVKLLGLQANVCFAGLVRELPPLYVRSELVVMPSFYEP
                     LGMSQIEALGLGVPVVASRVGGIPETVHERRTGLLAAPGDMEAWTEALSWALAHRAEM
                     RAMAQAGRADVRQRFELAKVLDELAGHLV"
     gene            2086..>2980
                     /locus_tag="VFX94_04970"
     CDS             2086..>2980
                     /locus_tag="VFX94_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX5767569.1"
                     /translation="MEPFGGESLRLYRRLLSYVRPYAKVFAVAVLGMVAAAATEPLFP
                     ALIKPLLDGGFATGGPTYPPLVFAGGIVAVFVVRGIFTFTSSYCLTWVANKVVLDLRA
                     AMFGRLVRLPAKFFDDNSSGALLSKVAYDVSGVAGAATTVLTVLVKDTIAIVGLLAWL
                     FYLNWKLTLIALAIGPPVALTVRLISTRMRRMSREALRSLGDVVHVLQETIDCHKVVK
                     VFGGQDYELGRFHRSAQVLRGFIMRADVPAALTTPITHTFTAFALAVIIYISMQDTLA
                     SRATVGEFASFITAMLMLLAPL"
BASE COUNT          506 a         1011 c          977 g          486 t
ORIGIN      
        1 gcggcagtca gcaagtattg gttatgcgga ttgttcgctt ccggcagacc ggtgccgcgg
       61 atcttctcgc ggtaggcagt gatgaagccg ccagtgccaa caccaatgac gggatggtcc
      121 cggatgatcg cagccgtcgt cttgtagtac gtcatgcgta cgccggtaga gctttccgga
      181 ttcacgcgcc ccgcattcca cgcgcggagc tccgccaagg cgaacgtgct gcggccaatg
      241 agcggcgcgt ctgccatgta ggccaccgca agcccgagca cgcctgcaaa ggctgctgcg
      301 gccacccccc gccagcgcca atgagccgcg cagaggtata gccacagcag agcgcagacc
      361 aggtggccgg tacgcccctg caccatgaag acgttggaag cggcgagcac tgcggcgacc
      421 gcataagcga tgcgccagcc cgaccgcgct gcgcgcaatg cccgatccgc cgcaatgaac
      481 gaggcgagcg ccatcagcac gccgtgtgtg atgtgaagct tgaatgccgt cggatttccc
      541 acatcgcggc caaaatgccc atcgactggc acaagcccca gccagatcgc ggaagacacc
      601 acgaacgtga gcgccatcgc ggcaaggaaa gcattcaagg cgaggtgctt gtcgcgctcg
      661 cgcaggacga atgcgcccag gacggcaaca agcagcagct ctttgtactt gttcatgtaa
      721 taccagccgt cgcgcgcgct accgggaccc catgccatgc cgaggagcag cagccccagc
      781 agggcgagcg ccgcaagcgc cgcggggttc tgccggatgg atgcgagctt ctcgcgcagg
      841 tggccgctcg ccagccaggc gatgaggatg agcgcgaaga gcacgttgtc cagcgcggtc
      901 gaaatcggga tcgatacgcc catcgcgatg gcggcatagc gccccaggtt gtgaaaacta
      961 gacgagatgg ccggcaagct cgtccaggac tttggcaagc tcgaagcgtt gccggacgtc
     1021 ggcccggccg gcttgcgcca tggcgcgcat ctcggcgcgg tgcgcgagcg cccaggagag
     1081 cgcctcagtc caggcctcca tatctccggg cgctgcaagc aggccggttc ggcgctcgtg
     1141 cactgtctcg gggatcccgc ctacccggct cgcgacgacc ggcacaccga gcccgagcgc
     1201 ttctatctgg gacatgccga gaggctcata gaaagacggc atcacgacaa gctcgctgcg
     1261 cacatagagc ggcggtagct cgcgcacgag ccccgcgaag cacacgttcg cttgcagccc
     1321 aagcagcttc acctgctggt cgattctttc tttcaattcg ccttcgccgg cgatcacata
     1381 acacaattcg ggaacgctct tccgcagccg cgccagaacc tcgagcatga agaggtgtcc
     1441 cttttccccg cgcagcatgg ccgcgtgcgt caggcggcgc ggcgggaacc ggctgagcgc
     1501 ctgctcgagc gctgcatcga gtggcagtgc ggcgcgtgcg tcgagctcgg cgaaagcgat
     1561 gccggggcga agcaccgcga tccgctccgc gcccacgcgc ggattggcga gcaggcgggc
     1621 gcgcatttcc tcgcttggca ccagtgtccg gtcgaacaga cggttgtacg tccacgcgtg
     1681 cggtgagccg tgctggtagg tgcgcatgcg gacgagccgc ggacgcggac ggcctaccag
     1741 gcgcgccgcg agcgccgaaa cgtcggcgtc gtgcccgcta tgacagagga ctgcgtcggg
     1801 gcgccagtcg gcgagcacgc gccttacgcc gagcacgctg cgcgggtgca cgctgttgcg
     1861 gaaaggtacc gccgtcaccg gcacgcggta acgcacggca agttcggcga tgcggctcgt
     1921 ttccgggcag agcagccgcg tctgcacgcc gcggccagca aggccgcgcg cctgctccag
     1981 aagctgcaat tcctgaccac ctatattggg cgagctctcg gtgaacagca cacgcgccag
     2041 cttgggcacg gcgcatttta ccgagcgctt acaatcgggg ccgtcatgga acctttcggc
     2101 ggcgaaagcc tcaggctgta ccggcggctg ctctcctacg tgcgcccgta cgcgaaagtg
     2161 ttcgcggtcg cggtgctcgg catggtggcg gccgcggcga cggagccgct ctttccggcg
     2221 ctgatcaagc cgctcctcga cggcggtttc gccaccggcg ggccgacgta tccgccgctc
     2281 gttttcgcgg gcgggatcgt agcggtcttc gtcgtgcgcg gcatcttcac cttcacctcc
     2341 tcctattgcc tcacctgggt ggcgaacaag gtggtgctcg acctgcgcgc cgccatgttc
     2401 gggcgcctgg tccggctgcc ggcgaagttc ttcgacgaca acagctccgg ggcgctgctt
     2461 tccaaggtgg cctacgacgt ctccggcgtg gcgggcgcgg ccaccacggt gctgaccgtg
     2521 ctcgtcaagg acaccatcgc gatcgtcgga ctgctcgcgt ggctgttcta cctgaactgg
     2581 aagctcaccc tgatcgccct ggcgataggc ccaccggtgg cgcttacggt gcggctcatt
     2641 tcgacccgca tgcggcgcat gtcgcgcgaa gcgctgcgct cgctcggcga cgtggtccat
     2701 gtgctgcagg agacgatcga ctgccacaag gtggtcaagg tgttcggcgg ccaggactac
     2761 gagctcggcc gctttcacag gagcgcgcag gtcctgcgcg gcttcatcat gcgcgccgac
     2821 gtcccggcgg cgctcaccac gccgatcacc cataccttca ccgccttcgc gctggcggtg
     2881 atcatctaca tctcgatgca ggacacgctc gcctcgcgcg ccaccgtagg cgagttcgcc
     2941 tccttcatca ccgcgatgct gatgctgctc gcgccgctca
//
LOCUS       DASUNT010000252         3561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_386034, whole genome shotgun sequence.
ACCESSION   DASUNT010000252 DASUNT010000000
VERSION     DASUNT010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386034"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFX94_04975"
     CDS             complement(<1..466)
                     /locus_tag="VFX94_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEX5767570.1"
                     /translation="MSQPPAHPPSIDGFEFAAAGAKQQGVWPLSDFPRLRDMLAADAG
                     EVAYEISGVRDERGRPGLRLKVSGTLTLRCQRCLEPMAFKVQTDETLVLAATLAEIHD
                     EPADALGPDRVVAGKEMALRELIEDELILAVPYAPRHESCTAGGADDREERTS"
     gene            515..1096
                     /locus_tag="VFX94_04980"
     CDS             515..1096
                     /locus_tag="VFX94_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HEX5767571.1"
                     /translation="MEQSRRLVLASASRYRKELLERLGLAFEVFSPEVDETPLGGELP
                     ADTALRLSLLKAAAAAKRGELIIGADQVASCQGRRYDKPGTYDNAVCQLRELSGKTVE
                     FHTAVTLLDSTKKTHESQVVPCRVVFRPLDERRIQAYVGREMPYDCAASAKAEGLGIA
                     LIERIETDDPSSLIGLPLIALTGMLERAGMPVL"
     gene            1159..1767
                     /locus_tag="VFX94_04985"
     CDS             1159..1767
                     /locus_tag="VFX94_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5767572.1"
                     /translation="MSTVRGIKDFVVENAKSARAFLGALEMPVRELNIRLIGEERDPL
                     APLRHQRPLGLLSDAGCPAIADPGARVVEAAHREGFRVVPLVGPCAIVLALMGSGLEG
                     QRFAFCGYLPREAADRARRIRALEQRSRREKETQIFIETPYRNDVLLAALLDGCGPTT
                     RLCVAAGLTLQEESITTRPIAEWRRAKALVGRRPAVFLLLAD"
     gene            complement(1756..2694)
                     /locus_tag="VFX94_04990"
     CDS             complement(1756..2694)
                     /locus_tag="VFX94_04990"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEX5767573.1"
                     /translation="MTENSGQWERELVTRLATAALKEQRRARLWGIFFKLLTFAYVTL
                     VLLMAVDWKSADVAGGKKHTAMVEVNGLIAPGADASAEKITMALQAAFKDKNTQGVVV
                     RINSPGGSPVQAQTIYDEMKRLRTKYPEIPLYAVVEDVCASGGYFVAVGADQIYVGKA
                     SIVGSIGVLMNGFGFTGLMEKLGVERRLVTAGENKGMLDPFSPVDPKDVEHVKKLMGD
                     IHQQFIGVVREGRGKRLKDSPEIFSGLIWTGERSIDLGLADGFGSLEYVAREVIKAED
                     IRDFTISEGIAEKFARRFGAAAANSIVESLLRASIR"
     gene            complement(2687..3043)
                     /locus_tag="VFX94_04995"
     CDS             complement(2687..3043)
                     /locus_tag="VFX94_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5767574.1"
                     /translation="MAAGERLICEAAALADAGRGVRFEVEYFGEPAAAFVVRKDGKVH
                     GYLNRCAHVAMELDWQEGAFFDADGRDLLCSTHGATYDAATGRCLGGPCNGSPLVKLR
                     LEERDGRVYFMGFEDD"
     gene            complement(3022..>3561)
                     /locus_tag="VFX94_05000"
     CDS             complement(3022..>3561)
                     /locus_tag="VFX94_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX5767575.1"
                     /translation="PELERARHVIGLGLQDAMRIVVPQLPARRYPEFVARYREHFTAR
                     KDSMQIFAGMRELLTELAGKHLLAIATGKSRRGLDRDIEFHGLKALFAASRCADETRP
                     KPDPAMLAELMAELAVQRHASLMIGDTSHDMEMARAAQVDALAVAYGAHPADGLRACG
                     PLGCFSTVDELQAWLQANG"
BASE COUNT          556 a         1258 c         1166 g          581 t
ORIGIN      
        1 gcgaagtcct ttcctcccgg tcatccgccc cacccgcggt gcagctctcg tgccgcggag
       61 cgtacggcac cgcgagaatc agctcgtcct cgatcagctc tcgcagcgcc atctccttgc
      121 ctgcgaccac ccggtccggg ccgagcgcgt cggccggctc gtcgtggatc tcggcgagcg
      181 tggcggcgag caccagcgtt tcgtccgttt gcaccttgaa cgccatcggc tccaggcagc
      241 gctggcagcg cagcgtcagc gtgccgctca ccttaagccg caggcccggc cgcccgcgct
      301 catcgcgcac tccgctgatc tcgtacgcca cctcgcccgc gtcggcggcg agcatgtcgc
      361 gcaggcgcgg aaaatcgctc agcggccaga ctccctgctg ctttgcgccg gctgccgcga
      421 actcgaagcc gtcgatgctc ggcggatgcg ccggcggttg cgacataaac gcgcgatgat
      481 atcatcgcgg taaaagcccc gtcaaaacaa agtgatggaa caatcccggc gactcgtgct
      541 ggcgtccgca tcgcgctacc gaaaggagct gctcgagcgg ctcgggttgg cgttcgaagt
      601 gttctcgccc gaggtcgatg aaacgccgct cggcggcgag ctgcccgccg acactgccct
      661 tcgtctttcg ctcctcaagg cagcggcggc tgcgaaacgc ggcgagctca tcatcggagc
      721 cgaccaagtc gcctcctgcc aaggccggcg ctatgacaag ccgggtacct acgacaacgc
      781 cgtgtgccag ctgcgcgagc tgagcgggaa gacggtcgag ttccacactg ccgtaacgct
      841 gctcgattcg acgaagaaaa cccacgagtc gcaagtggtc ccttgccgcg tcgtgttcag
      901 gccgctggat gagcgccgga tccaggccta cgtcgggcgc gaaatgccct acgactgcgc
      961 ggcgagcgcc aaggccgaag gcctgggcat tgcgctcatc gagcgcatcg agaccgatga
     1021 cccgagctcg ctcataggct tgccgctcat cgcgctgacc ggaatgctcg agcgcgccgg
     1081 gatgccggtg ctttgatcct ctacgccatc ccgacgcctc tgggtggttc tcccgcggga
     1141 gcgctccccg ggcccagcgt gagcaccgta agaggaatca aggattttgt ggtcgagaac
     1201 gccaagagcg cgcgggcgtt tctcggcgct ctggaaatgc cggtgcgcga gctcaacatc
     1261 aggctgatcg gagaagagcg cgacccgctc gcgccgctca ggcaccagcg cccattgggc
     1321 ctgctctccg acgcgggctg cccggcgatc gccgaccccg gcgctcgggt ggtcgaggcg
     1381 gcgcatcgcg aaggcttccg cgtcgtgccg ctcgtaggcc cctgcgccat cgtgctcgcg
     1441 ctgatgggct cggggctcga aggacaacgc ttcgcgtttt gcggctactt gccgcgcgag
     1501 gcggctgacc gagcgcgcag aatccgcgcg ctggagcagc gttcgaggcg cgagaaggaa
     1561 acccagatct tcatcgagac gccctaccgc aacgacgtgc tgctcgccgc gctgctcgac
     1621 ggctgcgggc cgactacgcg gttgtgcgtc gccgccggtc tgacgctcca ggaggaaagc
     1681 ataaccacca ggccaatcgc ggaatggcgc cgggcaaagg cgctcgtcgg ccggcgcccg
     1741 gcggtgtttc tgctgctagc ggattgaggc gcgcagcagc gattcgacga tcgagttcgc
     1801 ggcggcggcg ccgaagcggc gtgcgaactt ctccgcgatg ccctcgctga tcgtgaagtc
     1861 gcggatgtcc tcggccttga tcacttcgcg cgccacgtat tcgaggctgc cgaagccgtc
     1921 ggcgagcccg aggtcgatgc tgcgctcgcc ggtccagatc aggccgctga agatctcggg
     1981 cgagtccttg agccgcttgc cgcgaccctc gcgcaccacg ccgatgaact gctggtggat
     2041 atcgcccatc agcttcttga cgtgctcgac gtccttcggg tcgaccggag agaacgggtc
     2101 gagcatgccc ttgttctcgc cggcggtgac caggcggcgc tccacgccaa gcttctccat
     2161 cagcccggtg aagccgaagc cgttcatcag cacgccgatc gagcccacga tgctcgcctt
     2221 gccgacgtag atctggtcgg cgccgacggc gacgaagtag ccgccggagg cgcacacgtc
     2281 ctcgaccacc gcgtagagcg ggatctcggg gtatttcgtg cgcaggcgct tcatctcgtc
     2341 gtagatcgtc tgcgcctgca ccggactgcc gccgggcgag ttgatgcgca ccaccacccc
     2401 ctgggtgttc ttgtccttga acgccgcctg cagcgccatg gtgatcttct ccgcgctcgc
     2461 gtcggcgccg ggcgcgatca gcccgttcac ctccaccatc gcggtgtgct tcttgccgcc
     2521 ggccacgtcc gcgctcttcc agtccaccgc catcagcagc accagcgtca cgtaggcgaa
     2581 ggtcaggagc ttgaagaaga tgccccacaa ccgggcgcgg cgctgctcct tgagcgccgc
     2641 ggtggcgagc cgggtgacga gctcacgttc ccactgacca ctgttctcag tcatcttcga
     2701 atcccatgaa atagacgcga ccgtcgcgct cctcgaggcg cagcttcacc agcgggctgc
     2761 cgttgcacgg ccctcccagg cagcggccgg tggccgcgtc gtaggtggcg ccgtgcgtcg
     2821 agcatagcaa atcgcgtccg tcggcatcga aaaatgcacc ttcctgccag tcgagctcca
     2881 tcgcgacgtg cgcgcagcgg ttgaggtagc cgtgcacctt gccgtccttg cgcaccacga
     2941 atgccgcggc cggctcgccg aaatattcga cctcgaagcg cacgccccgc ccggcatcgg
     3001 cgagcgccgc cgcctcgcag atcagccgtt cgcctgcagc cacgcttgca gctcgtccac
     3061 cgtggagaag cacccgagcg gcccgcaggc gcgcagcccg tccgcgggat gcgcgccgta
     3121 ggcgacggcg agcgcatcga cttgcgcggc gcgcgccatc tccatgtcgt gggaggtgtc
     3181 gccgatcatg agcgatgcgt ggcgttggac cgcgagctcg gccatcagct ccgccagcat
     3241 cgccgggtcc ggcttgggcc gagtctcgtc cgcgcagcgc gacgccgcaa acagcgcctt
     3301 gagcccgtgg aactcgatgt cgcggtcgag gccgcgccgg ctcttcccgg tggcgatggc
     3361 gagcagatgc ttccccgcaa gctcggtcag gagctcccgc atgccggcga agatctgcat
     3421 cgagtccttg cgcgcggtga agtgctcgcg gtagcgcgcg acaaactccg gatagcggcg
     3481 cgccggcagc tgcggcacca cgatgcgcat cgcgtcctgc aggccgagcc cgatcacgtg
     3541 gcgcgcgcgc tcaagctcgg g
//
LOCUS       DASUNT010000253         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_648517, whole genome shotgun sequence.
ACCESSION   DASUNT010000253 DASUNT010000000
VERSION     DASUNT010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648517"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VFX94_05005"
     CDS             complement(<1..664)
                     /locus_tag="VFX94_05005"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEX5767576.1"
                     /translation="MQAGIRYTVATGEKLVNETFGPNNIRRRTSGTEEVFPMEIEDGR
                     NRSDFSLDRNGFVLIEHRTAVKDFFDKAELERVYYAEVAALIGQLCGAKRVHVFDHTL
                     RSGDEAEREARLLREPVLSAHNDYTERSAPNRVREIMGEEADSLLRRRFAIVQVWRAI
                     NRPIVSNPLAIADARSICAEDLLVAERRYPHRVGETYRLRHNPGHRWFYFPKMRRDEA
                     LVF"
     gene            complement(688..981)
                     /locus_tag="VFX94_05010"
     CDS             complement(688..981)
                     /locus_tag="VFX94_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767577.1"
                     /translation="MDSAEAELEGRIARVFARRPELWRFTVRDRSGLPDHIDPTTLAG
                     EFFIFEIGLSPRRDRAHYDEVYQDIARAIAAAVAAQPAARKLLPGRTFVRALH"
     gene            1018..>2015
                     /locus_tag="VFX94_05015"
     CDS             1018..>2015
                     /locus_tag="VFX94_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEX5767578.1"
                     /translation="MEHGNDFLVQASLYFAAAVAAVMISHKFGLGSVAGYLLAGIAIG
                     PWGFQLVEAGAHIKGFAELGVAFLLFVIGLELEPKRLWSMRAKLLGLGLSQVLGSIAL
                     IGTAGWALGLEPRVALIAAMALSLSSTALALAPLTERGALATQGGQGTFAVLLFQDIA
                     AIPLLALVPFLAEVPGGSSGLDAMAALKVLGAVVLVVVIGRFLTRPVLRVVARTGQRE
                     VFSAFTLLLVLGIAQLMASVGISMALGAFLAGVLLAGSEYRHALETDIEPFRGLLMGL
                     FFIAVGMSVDFGLLARQPLFVLGLVLGFQLLKVLALRAVAGPLGVTTRQRWLIAALL"
BASE COUNT          258 a          683 c          700 g          374 t
ORIGIN      
        1 tgaacaccag cgcctcgtcg cggcgcatct tcgggaagta gaaccagcga tgacccgggt
       61 tgtgcctgag acgatacgtt tcgcccacgc ggtgcggata gcgccgctcg gcgacgagca
      121 ggtcctcggc gcagatgctg cgcgcatccg cgatcgcgag cggattcgat acgatcggcc
      181 ggttgatcgc ccgccacacc tggacgatgg cgaaccggcg ccgaagcagc gagtccgctt
      241 cctcgcccat tatttctctc accctgttgg gtgcggaccg ctcggtgtag tcgttgtgcg
      301 ccgaaagcac gggctcgcgc aggagccgcg cctcgcgctc ggcctcgtcg cccgagcgca
      361 gcgtgtggtc gaagacgtgc acccgcttcg ctccgcaaag ctgcccgatc agcgccgcga
      421 cctccgcgta atagactcgc tcgagctcgg ccttgtcgaa gaagtccttt acggccgtgc
      481 ggtgctcgat gagcacgaac ccgttccggt cgagcgaaaa gtcgctgcgg ttgcgcccgt
      541 cctcgatttc catcgggaaa acttcctccg tgcccgaagt gcggcggcgg atgttgttcg
      601 gcccgaaggt ctcgttgacg agcttctcgc ccgtggccac ggtgtagcgg atgccggcct
      661 gcaccgcgta actataaacg caggcggcta gtgcagggct ctcacgaagg tgcgtcccgg
      721 caggagcttg cgcgccgcgg gctgggcagc caccgcggcg gcgatggcgc gcgcgatgtc
      781 ctggtatacc tcgtcgtagt gcgccctgtc gcggcgcggc gaaaggccga tctcgaagat
      841 gaaaaactcg ccggcgagcg tggtcggatc gatgtgatcc ggcagcccgc tgcggtcgcg
      901 cacggtgaag cgccacagct cggggcggcg cgcgaagacc cgcgcgatcc gcccctcgag
      961 ctccgcttcg gcggagtcca tttctttcaa ccctcccgcc gccaatatac ttcggccgtg
     1021 gagcacggca acgattttct ggtccaggcg agcctgtatt tcgccgccgc ggtggccgcc
     1081 gtgatgattt ctcacaagtt cggtctgggc tcggtggcgg gctacctcct cgccgggatc
     1141 gcgatcgggc cgtggggctt ccagctcgtc gaggccggcg cgcacatcaa gggcttcgcc
     1201 gagctcggcg tggcgttcct gctcttcgtg atcggcctgg agctcgagcc gaagcgactg
     1261 tggagcatgc gcgcgaagct cctcggcctg gggctttccc aggtgcttgg ctcgatcgcg
     1321 ctcatcggca ccgccggctg ggcgctcggc ctcgagccgc gcgtcgcgct gatcgccgcg
     1381 atggcgcttt cgctttcctc gaccgcgctc gcgctcgcgc ccctcaccga gcgcggcgcg
     1441 cttgccacgc aaggcggcca gggcaccttc gcggtgctgc tcttccagga catcgccgcc
     1501 attccgctgc tcgcgctcgt gccgttcctg gccgaggtgc ccggcggctc gagcgggctc
     1561 gacgcgatgg cggcgctgaa ggtgctgggc gcggtggtgc tggtggtggt gatcgggcgc
     1621 tttctgacgc ggcccgtgct gcgcgtggtg gcgcgcaccg gccagcgcga ggtgttcagc
     1681 gcgttcacgc tgctgctcgt gctcggcatc gcccagctga tggcgagcgt cggcatctcg
     1741 atggcgctcg gcgccttcct ggccggcgtg ctgctcgcgg gctccgaata tcggcacgcg
     1801 ctggagaccg acatcgagcc cttccggggg ctgctcatgg ggctgttctt catcgcggtg
     1861 ggcatgagcg tcgacttcgg gttactcgca cgccagccac tgttcgtgct cggcctggtg
     1921 ctcggctttc agctgctgaa ggtgctggcg ctgcgcgcgg tggcaggccc gctcggcgtc
     1981 acgaccaggc agcgctggtt gatcgcagcg ctgct
//
LOCUS       DASUNT010000254         2157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_386074, whole genome shotgun sequence.
ACCESSION   DASUNT010000254 DASUNT010000000
VERSION     DASUNT010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386074"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFX94_05020"
     CDS             <1..214
                     /locus_tag="VFX94_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5767579.1"
                     /translation="LFAGFMGAAASAGWFTAFAIAPAAHVRTLGLVELVFSYAVSRRI
                     FRERLTRLELAGMALLALGVAVITIA"
     gene            complement(247..660)
                     /locus_tag="VFX94_05025"
     CDS             complement(247..660)
                     /locus_tag="VFX94_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5767580.1"
                     /translation="MSRLPTVREFMDRHVQTISPEADIMEAVDFLLEKRVTGALVADN
                     KGKLVGILTEFDCLRLLTHGDERAEAPRGKVKDFMTSDVQTIPPTMDIYYCAGLFMNV
                     SFRRFPVVENGRIVGAVTRFDLLRAVRHAFAQEPL"
     gene            742..1698
                     /locus_tag="VFX94_05030"
     CDS             742..1698
                     /locus_tag="VFX94_05030"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5767581.1"
                     /translation="MTKSKVLLLCAAFVLALPARAQTYPIKPVRIVVPFAPGTATDNL
                     VRPLADVMAASLKQPVVLDNRVGAVGKIAAEHVARSAPDGYTVLAGATTVIAANPALF
                     KSLSYNPRTDFDAVIGLGTIPQVVVVHPSIPAKNMAEFVEYAKQNPGKLFYASGTAGN
                     IIPSRILSDRLGLGMTNVSYKSPPQALTDVVAGRVPMMAVDVSAVLGQIRGGSVRPLA
                     VTSSKESPVLPGVPPLADTLPGFELIVFFGLYVPKGTDPAIIQALYQAARAAAERDEF
                     KRSLAGQGFEIRTSDPAELAAFSAREIERWAQMVKEAKIDPE"
     gene            1745..>2157
                     /locus_tag="VFX94_05035"
     CDS             1745..>2157
                     /locus_tag="VFX94_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767582.1"
                     /translation="MLRPVALFAALLVAGQALAHEEEGKSSGQVLGKVNFKTSCSKAV
                     QPKFNRAVALLHSFWYTESEAAFNEVAAEEPSCTIAYWGVAAILMNNPIAGQGAVGKG
                     PEKAQAALEKARANPPKTQRERDYIEAVSAYYKDFG"
BASE COUNT          358 a          700 c          724 g          375 t
ORIGIN      
        1 ccttttcgcc gggttcatgg gcgctgccgc gtcggcgggg tggttcaccg cctttgcgat
       61 cgcgcccgcc gcgcacgtgc gcaccttggg cctcgtcgag ctcgtcttca gctacgcggt
      121 gtcacggcga attttccgcg agcgcctgac gcgcctcgag ctcgccggca tggcgctcct
      181 tgcgctcggc gtggcggtca tcaccatcgc ctgagcgcgc cgagggcagt tacgaagacc
      241 gcagcgttac agcggctctt gcgcgaaagc gtggcgcacg gcgcgcagca ggtcgaagcg
      301 ggtgacggcg ccgacgatgc ggccgttctc gaccaccggg aagcgccgga acgagacgtt
      361 catgaagagg ccggcgcagt agtagatgtc catggtgggc gggatggttt gcacgtcgga
      421 ggtcatgaag tccttcacct tgccgcgggg cgcttcggcg cgctcgtcgc cgtgcgtaag
      481 aagccgcagg cagtcgaact cggtcagaat tccgaccagc tttcctttgt tgtccgccac
      541 cagcgcgccg gtgacgcgct tctccagcag gaagtcgacc gcttccatga tgtcagcttc
      601 cggcgagatg gtctgcacgt ggcggtccat gaattcgcga acggtgggaa ggcggctcat
      661 tggttcctcc ttgggtgcga gccaggaggg gtcatttttg ctgctacgct cgcggctatc
      721 aagcccgaga ggaggggatt catgacgaaa agcaaggtgc tgcttttgtg cgctgcgttc
      781 gtgctggcgc tgcccgcgcg ggcgcagacc tacccgatca agccggtccg catcgttgta
      841 ccgttcgcgc cgggcaccgc gaccgacaac ctggtgcggc cgcttgctga cgtgatggcg
      901 gcgagcctca agcagccggt ggtgctggat aaccgcgtcg gcgccgtggg gaagattgcc
      961 gccgagcatg tcgcccggtc ggcgccggac ggctacaccg tgctggccgg cgcaaccacc
     1021 gtgatcgcgg ccaacccggc gctcttcaag agcctcagct acaacccgcg caccgacttc
     1081 gacgcggtca tcggcctggg cacgataccg caggtcgtgg tcgtgcatcc gagcattccg
     1141 gcgaagaaca tggccgagtt cgtcgagtac gcaaagcaga atcccggcaa gctgttttac
     1201 gcctcgggga ccgcgggcaa catcattccc agccgcattc tttcggaccg gctcggcctg
     1261 ggcatgacca acgtttccta taagagcccg ccacaggccc tgaccgacgt cgtggcgggc
     1321 cgtgtaccga tgatggccgt ggatgtttcc gcggtccttg gccagatccg gggcggaagc
     1381 gtgcgcccgc tcgccgtcac gtcgtcgaag gaatcgcccg tgctccccgg cgtgccgccg
     1441 ctcgccgaca cgctgccggg cttcgagctg atcgtgttct tcggccttta cgttcccaag
     1501 ggcaccgatc ccgcgattat tcaggcgctc taccaggcgg cccgcgccgc agccgagcgc
     1561 gacgaattca agcgcagcct ggcgggccag gggttcgaga tccggaccag cgatcccgcc
     1621 gagctcgccg cattctcggc acgcgagatc gagcgctggg cgcaaatggt gaaggaagcg
     1681 aagatcgatc cggagtaagg gttcgggagt agactttccg tcccacctat tggaggagtt
     1741 tcgcgtgcta cgtccagtcg cccttttcgc agccttgctc gtcgccgggc aggccctcgc
     1801 gcacgaggag gaaggaaaat cctcaggcca ggtcctcggc aaggtgaact tcaagacctc
     1861 ctgcagcaag gcggtgcagc cgaagttcaa ccgcgcggtc gcgctgctgc attcgttctg
     1921 gtacacggag tccgaggcgg cgttcaacga ggtcgccgcc gaggagccga gctgcacgat
     1981 cgcgtactgg ggcgttgccg cgatcctcat gaacaacccg atcgccggcc agggcgcggt
     2041 cggaaaaggt cccgagaagg cgcaggccgc gctcgagaag gcgcgcgcca atccgcccaa
     2101 gacgcagcgc gagcgcgact acatagaggc agtgtccgcc tactacaagg acttcgg
//
LOCUS       DASUNT010000255         2139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_911264, whole genome shotgun sequence.
ACCESSION   DASUNT010000255 DASUNT010000000
VERSION     DASUNT010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911264"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..937
                     /locus_tag="VFX94_05040"
     CDS             308..937
                     /locus_tag="VFX94_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5767583.1"
                     /translation="MRILIADDHPIVRHGLKQMLASDPSATVVGEATNGDEALEMARK
                     LEWDMAIFDFTMPGRSGLDLLGDMKKEFPGRPVLILSMHSEDVHGSRVLRGGAAGYIT
                     KDSAPQELATAVRKVANGGKYVSASLAEILASECSSDPQKALHEKLSDREYRVMWLLA
                     TGKQISEIAKEMGLSPSTVSTYRTRILRKLGLTSNAALVHYAVRHQLVG"
     gene            948..2051
                     /locus_tag="VFX94_05045"
     CDS             948..2051
                     /locus_tag="VFX94_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5767584.1"
                     /translation="MNDLAHAPAIDLASQEAHDKAKILLVDDEPKSLFALQELLATLG
                     QNLMVAQSGEEALRLALRNDFAVILLDVRMPGIDGFETARLIRARERSKLTPIIFLTA
                     AADEMTSMFRGYEVGAVDYLMKPVIPDVLKSKVAVFVELFRKSERLRESEDKLRRLAA
                     HLISVREEERAHIAREIHDELGQVLTGLKMEVTWLAKRLKDKPLVEKTDTMCALIDST
                     VQTVRKIATGLRPEMLDDMGLVAAVGWQAKEFQKRTGIRCRASLPPEARLDIDVSTTM
                     FRILQEILTNVARHSRATRVDIELAIRESAVSLEVVDNGIGIQDSELTAKKSLGLLGM
                     QERALLFGGDVSITGSPGHGTRVSVSIPLRKPQ"
BASE COUNT          398 a          693 c          700 g          348 t
ORIGIN      
        1 tacaccgtgt aggcgagacg gctacagatg tgtagtggcg caccgacaag tgagaaggct
       61 gaaaagctca agtcgctgtt tccgcgcccg atcgaatgtc gctccgcagc gaccgtggtt
      121 cgcgcgtttg gggattcaga aaggcaagac tggcgttttt ccgcgcggtg tcgaagcccg
      181 actacacgcg tgtcaaccca ttgctacgtc gaaaaaaatt cctctgcgag tcccgcgcgg
      241 caatggtcaa tcgcagacgg cgtgaatata ctttccgacc accgccggat tttcgtaggg
      301 agactacatg cgaatactca ttgcagacga ccatccgatc gtgcggcacg ggctgaagca
      361 gatgctcgcg agcgatccgt cagcgacagt cgtaggcgag gctacgaacg gcgacgaagc
      421 gctcgagatg gcgcgcaagc tcgagtggga catggcgatc ttcgacttca ccatgccggg
      481 gcgcagcggg ctcgatctcc tcggcgacat gaagaaggag tttcccgggc gtccggtgct
      541 catcctgtcc atgcattcgg aggatgtgca cggcagccgc gttttgcgcg gcggcgcggc
      601 cggctacatc accaaggaca gcgcgccgca ggagctcgcg accgcggtgc gcaaggtggc
      661 gaacggcggc aagtacgtgt ccgcctcgct cgccgagatc ctcgcctccg agtgctcgag
      721 cgatccgcag aaggcgctgc acgagaagct ttccgaccgc gagtaccgcg tgatgtggct
      781 gctcgccacg ggcaagcaga tcagcgaaat cgccaaggag atggggcttt cgccctccac
      841 ggtgagcacg tatcgcacgc gcatcctgcg caagctgggg ctgacgagca acgccgccct
      901 ggtccactac gcggtccgcc atcagctggt gggatgaggc gggcagcgtg aacgatctcg
      961 cgcacgcacc cgctatcgat ctagcgagcc aggaagcgca cgacaaggcg aagatcctgc
     1021 tcgtcgacga cgagccgaag agcctgttcg cgctgcagga gctgcttgcc acgctcgggc
     1081 agaacctcat ggtggcgcag tcgggcgagg aggcgctgcg ccttgcgctg cgcaacgact
     1141 tcgcggtgat cctgctcgac gtgcgcatgc cgggcatcga cggcttcgag accgcgcgcc
     1201 tcatccgcgc gcgcgagcgc tccaagctca cgccgatcat cttcctcacc gccgcggccg
     1261 acgagatgac ctccatgttc cgcggctacg aggtcggcgc ggtcgactac ctgatgaagc
     1321 cggtgatccc ggatgtgctc aagtcgaagg tcgcggtgtt cgtcgagctt ttccgcaaga
     1381 gcgagcggct gcgcgagtcg gaggacaagc tgcgtcgcct ggcggcgcat ctcatttcgg
     1441 ttcgcgagga ggaacgcgcc cacatcgcgc gcgagatcca cgatgagctc ggccaggtgc
     1501 tcacgggcct caagatggaa gtcacctggc tcgccaagcg gctgaaggac aagcccctcg
     1561 tcgagaagac cgacaccatg tgcgcgctca tcgactcgac ggtgcagacg gtgcgcaaga
     1621 tcgcgaccgg gctgcgcccc gagatgctcg acgacatggg cctggtggcc gcggtcggtt
     1681 ggcaagcgaa ggaattccag aagcgcaccg ggatccgctg ccgcgcgagc ctgccgcccg
     1741 aggcgaggct cgacatcgac gtctcgacca ccatgttccg catcctccag gagatcctca
     1801 ccaacgtggc gcgccactcc cgcgccacgc gcgtcgacat cgagctcgca atccgcgagt
     1861 cggcggtctc gctcgaggtg gtggacaacg gcatcggcat ccaggacagc gagctcaccg
     1921 ccaagaagtc cctcggcctc ctcggcatgc aggagcgggc gctgctcttc ggcggcgatg
     1981 tctccatcac gggctcgccc ggccatggca cgcgcgtgtc ggtctccatc cccttgagga
     2041 aaccgcaatg aaagttctca ttgccgacga ccatccggtc gtgcgtcagg gcctgcgcca
     2101 gatgctcgcc gtcgagtcgg acctgacggt ggtcggcga
//
LOCUS       DASUNT010000256         2562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_473455, whole genome shotgun sequence.
ACCESSION   DASUNT010000256 DASUNT010000000
VERSION     DASUNT010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473455"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFX94_05050"
     CDS             complement(<1..435)
                     /locus_tag="VFX94_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX5767585.1"
                     /translation="MKVPESWLRSFCNPPLPGRELAEKLTMSGLEVEVYEPVGPAFSG
                     VVVGEVLSVEKHPNADKLSVCAVSAGKETVQVVCGAPNVRAGMKAPLARIGTKEIRGV
                     QSHGILCSARDLKISDDHSGLVELARDAKPGTDARIALGLDDH"
     gene            complement(432..1445)
                     /gene="pheS"
                     /locus_tag="VFX94_05055"
     CDS             complement(432..1445)
                     /gene="pheS"
                     /locus_tag="VFX94_05055"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX5767586.1"
                     /translation="MEQLREQLSVIVTRALADFDAAGDPASLENAKARYLGKAGELAS
                     FQASLRALPVEQKKSAGAAYNAAKERIEGALEARRAALAEAKLAARLGEQALDVTLPG
                     RGRPRAGVHPVIRTWQRIEEIWRSIGFDVADGPEIETDWFNFTALNSPENHPSRSMQD
                     TFYVDLKDANGLPLVLRTHTSPMQVRYARMHKPPIKVIAPGRTYRVDSDATHSPMFHQ
                     VEGLWIDADISFADLKGVYTGFLRRFFENDDLEVRFRPSFFPFTEPSAEIDMKLKGRW
                     LEISGSGQVHPAVVRNFGLDPERYIGFAFGSGLERLTMLRYGIEDLRLFYEGDLRFLE
                     QFA"
     gene            complement(1460..1819)
                     /gene="rplT"
                     /locus_tag="VFX94_05060"
     CDS             complement(1460..1819)
                     /gene="rplT"
                     /locus_tag="VFX94_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX5767587.1"
                     /translation="MPRVKRGVTQRARHKKVLKQAKGFRGRRKNVFTVAKQAVMKAGQ
                     YAYRDRRRRKREFRALWIARINAAVRELGMSYSVFMAGLKRANIDIDRKVLADLAVMD
                     KPAFDKIAAQVKATIAH"
     gene            complement(1829..2029)
                     /gene="rpmI"
                     /locus_tag="VFX94_05065"
     CDS             complement(1829..2029)
                     /gene="rpmI"
                     /locus_tag="VFX94_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX5767588.1"
                     /translation="MPKMKSKRGAAKRFKVRPGGGIKRSQAYMRHILTKKSTKRKRHL
                     RGGTEVDHSNKAQILSVLPYSR"
     gene            complement(2117..>2562)
                     /gene="infC"
                     /locus_tag="VFX94_05070"
     CDS             complement(2117..>2562)
                     /gene="infC"
                     /locus_tag="VFX94_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX5767589.1"
                     /translation="NPPVVKILDVGKYKYQAQKKAAEARKKQKVVEVKEIKFRPMIDD
                     HDYDVKLRSMYRFFEDGDKVKVTLRFRGREMAHQEFGVRLLERVRKDLDAVGVVEQFP
                     RLEGRQMVMVLAPKKKGAGPAKGKAESAKPDKPKPAPAKIAAKSA"
BASE COUNT          393 a          857 c          814 g          498 t
ORIGIN      
        1 atggtcgtcc aggccgagcg cgatgcgggc atccgtgccg ggtttcgcgt cccgggcaag
       61 ctcgacaagc cccgagtggt catcggagat cttcagatcg cgcgccgagc acaggatgcc
      121 gtggctttgc acgccgcgga tttcctttgt gccaatcctg gcgagcggcg ccttcattcc
      181 ggcacgcacg ttgggcgcac cgcagacgac ctgcaccgtc tccttcccgg cggacaccgc
      241 acagacgctc agcttgtccg cgtttggatg cttttccacc gacagcactt cgccgaccac
      301 gacgccactg aaggccggtc cgacgggttc gtagacttcc acctcgagcc cggacatggt
      361 cagcttttcg gccagctcgc gccccggcag cggcgggtta cagaagctgc gcagccagct
      421 ttcgggaacc ttcatgcgaa ctgctccagg aagcgcagat ctccctcgta aaacaggcgc
      481 aggtcctcga tgccatagcg cagcatggtc aggcgctcga ggcccgagcc gaaagcgaag
      541 ccgatgtagc gctccggatc gagcccgaag ttgcgcacga cggcgggatg gacctgcccc
      601 gagccggaga tctcgagcca cctgcccttc aacttcatgt caatctcggc ggagggttcc
      661 gtgaagggga agaacgacgg ccggaagcgc acctcgaggt cgtcgttctc gaagaaacgc
      721 cgcaggaacc cggtatatac gcccttgagg tcggcgaagc tgatgtccgc gtcgatccac
      781 aggccctcca cctggtggaa catcggcgag tgggtggcgt cggagtccac gcgatacgta
      841 cgcccgggcg cgatgacctt gatgggaggc ttgtgcatgc gcgcatagcg gacctgcatc
      901 gggctcgtgt gcgtgcgcag gacaagcggc aggccgttcg cgtccttcag gtctacgtaa
      961 aacgtgtcct gcatggagcg cgacgggtgg ttctcggggc tgttgagcgc tgtgaagttg
     1021 aaccagtcgg tttcgatctc ggggccgtcc gcgacgtcga acccgatcga gcgccagatc
     1081 tcctcgatgc gctgccaggt acggatcacg ggatgcacgc cggcacgagg gcgtccgcgc
     1141 ccgggcagcg tgacatccag cgcctgctcg cccagccgcg cggcgagctt cgcttcggcg
     1201 agcgcggcgc gccgagcctc gagcgcgccc tcgatgcgct ccttggccgc gttgtacgcc
     1261 gccccggcgg atttcttctg ctcgaccgga agcgcgcgca ggctcgcctg gaacgaagcg
     1321 agctcccccg ccttgccgag gtagcgcgcc ttggcgttct ccagggaagc cgggtcgccg
     1381 gcggcgtcga agtcagcgag cgcccgggtg acgatgacgc tgagttgctc cctgagttgc
     1441 tccatctacg gcccgcaact cagtgagcga tcgtggcctt cacctgagcc gcgatcttgt
     1501 cgaacgcggg cttgtccatg acggcgaggt ctgcgaggac cttgcggtcg atgtcgatgt
     1561 tcgcgcgctt gagcccggcc atgaagacgc tgtagctcat gccgagctca cgcacggcgg
     1621 cgttgatccg cgcgatccag agagcgcgaa actcccgctt gcgccggcgg cggtcgcgat
     1681 aggcgtactg ccccgccttc attaccgcct gcttggcgac agtgaaaacg ttcttccggc
     1741 gcccgcggaa gcctttcgcc tgcttcagga ccttcttgtg gcgcgcgcgc tgcgttactc
     1801 cacgcttgac tcgcggcatg gcgcgctcct atcgcgagta gggcagaacc gacaggatct
     1861 gcgccttgtt ggagtgatcg acctcggtgc cgccgcgcag gtggcgcttt cgcttggtgc
     1921 tcttcttggt caggatgtgg cgcatgtagg cctgcgagcg cttgatgccg ccgcccggac
     1981 ggaccttgaa gcgcttcgcg gcgccccgct tggacttcat cttgggcacg attgccctcc
     2041 ttatctagct tgacgccggg cggtcccgcc tagcggagac gcttccacag ccgcggccat
     2101 cacttgtatt ccgccgtcag gcggactttg ctgcaatctt cgccggcgcc ggtttaggct
     2161 tgtccggctt ggccgactcg gctttccctt tcgccggtcc cgcgcccttc ttcttcggcg
     2221 ctagcaccat caccatctgc cggccttcga ggcggggaaa ctgctccacc acgccgaccg
     2281 cgtccaggtc cttgcgcacc cgctcgagca gccgcacgcc gaactcctgg tgcgccatct
     2341 cgcggccgcg gaagcgcaac gtgaccttca ccttgtcgcc gtcctcgaag aaacggtaca
     2401 tggagcgcag cttcacgtcg taatcgtgat cgtcgatcat cggccggaac ttgatttcct
     2461 tgacctcgac gactttctgc ttcttgcgcg cttcggcggc tttcttctgc gcctgatatt
     2521 tgtatttgcc gacgtcgaga attttcacga cgggcggatt gt
//
LOCUS       DASUNT010000257         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_824146, whole genome shotgun sequence.
ACCESSION   DASUNT010000257 DASUNT010000000
VERSION     DASUNT010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824146"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VFX94_05075"
     CDS             complement(<1..614)
                     /locus_tag="VFX94_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5767590.1"
                     /translation="MERIKDQQLAMMPIEFKLNGQTVIGRPDEPIITTAQKHGVEVPH
                     LCHGGNQRSDGNCRACVVEIKGERTLAPSCCRFPVAGMEVTTNSPRALASQKMVLELL
                     LSDMPEQEYTLDNKLKSWAKKIGVHKPRFPRREQPRSDLSHPAIAGCERSAFGCSRRA
                     KRGLPTSSAAAQSFSLLSSVYSCSGWSESRSSSTIFCEVSARSE"
     gene            complement(615..2261)
                     /locus_tag="VFX94_05080"
     CDS             complement(615..2261)
                     /locus_tag="VFX94_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX5767591.1"
                     /translation="MRKPRSTPKGRPVDPKAVSEVQALLGNESRQRDLLIEHLHKIND
                     RYGHLTAAHLAALAQEMRLALTEVYEVATFYHHFEVVKEGGEAPAAITVRVCDSISCE
                     LAGAQELLKKLSFGPGVRVIPAPCVGRCEVAPCVVVHQNALGNASVETVNAAVEAKKL
                     TCEEPKYIDYAQYKKAGGYKLIAECLAGKRTREDLTKIMEDSGLRGLGGAGFPAGRKW
                     RLVAAEPSPRVMALNIDEGEPGTFKDRWYLERDPHRFLEGMLVAAWAAGVGEIWIYLR
                     DEYAGCRAILERELARLIREAPCALPPIHLRRGAGAYICGEESAMIESIEGKRGMPRL
                     RPPYVAQVGLFGRPTLEHNMETVYWVREIVEKGAQWFAAQGRNGRKGLRSFSVSGRVK
                     KPGVHIAPAGITVLELIKEYCGGMLDGHEFYAYLPGGASGGILPASKGDIPLDFDTLQ
                     PHGCFIGSAAVVILSDRDKAAKAAVNLMKFFHDESCGKCTPCRVGTAKAVTLMEQRKW
                     NQPLLAELSQAMMDASICGLGQAAPNPALSVMKYFPQEVQ"
BASE COUNT          347 a          765 c          733 g          417 t
ORIGIN      
        1 aattccgagc gcgcgctgac ctcgcagaaa atggtgctcg agctcctgct ttccgaccag
       61 ccggagcagg agtacacgct cgacaataag ctgaagcttt gggccgcggc gctcgacgtc
      121 ggcaagccgc gcttcgcgcg ccgtgagcag ccgaaggccg acctctcgca ccccgcgatg
      181 gcgggatgcg agagatccga tctgggctgt tcccggcggg ggaaccgggg tttgtgcacg
      241 ccgattttct tcgcccagct cttcagcttg ttgtcgagcg tgtattcctg ctccggcatg
      301 tccgagagca aaagctcgag caccattttc tgcgaggcca gcgcgcgcgg cgaattggtc
      361 gtgacttcca tgccggccac cgggaagcgg cagcaggagg gcgcgagggt gcgctcgccc
      421 ttgatctcga ccacgcaggc gcggcagttg ccgtcggagc gctggttgcc gccgtggcaa
      481 aggtgcggga cctcgacgcc gtgcttctgc gcggtggtga tgatcggctc gtcgggccgg
      541 ccgatcaccg tctggccgtt cagcttgaac tcgatcggca tcatcgcgag ctgctggtcc
      601 ttgattcttt ccatctactg cacctcttgc ggaaagtatt tcatgacgct cagcgccggg
      661 ttcggtgccg cctggccgag gccgcagatc gacgcatcca tcatcgcctg cgacagctcg
      721 gcgagcagcg gctggttcca cttgcgctgt tccatcagcg ttaccgcctt cgcggtgccg
      781 acgcggcagg gcgtgcactt gccgcacgac tcgtcgtgga agaacttcat caggttcacg
      841 gcggctttgg cggccttgtc gcgatcgctc aggatgacca ccgcggcgga gccgatgaag
      901 cagccgtgcg gctgcagcgt gtcgaagtcg agcgggatgt cgcctttcga ggccggcagg
      961 atgccgccgg aggcaccgcc gggaagatag gcatagaact catgcccgtc gagcatcccg
     1021 ccgcaatact ccttgatcag ctccaatacc gtgatcccgg ccggagcgat atgcacgccc
     1081 ggctttttca cgcgccccga taccgagaac gagcgcaggc ctttgcggcc gttgcgtccc
     1141 tgcgcggcga accactgcgc gcccttctcc acgatctcgc gcacccagta caccgtctcc
     1201 atgttgtgct cgagcgtcgg ccggccgaac aggccgacct gcgccacgta cggcgggcgc
     1261 aggcgcggca tgccgcgctt gccctcgatc gactcgatca tcgccgactc ttcgccgcag
     1321 atataggccc ctgctcctct tctaagatga atagggggca gggcacaggg cgcttcacgg
     1381 atcaatctgg cgagttcccg ttccaggatc gcacggcagc cggcatattc atcgcgcagg
     1441 tagatccaga tctcgccgac gccggcggcc catgccgcca ccagcatgcc ctcgaggaag
     1501 cggtgcgggt cgcgctcgag gtaccagcgg tccttgaagg tgcccggctc gccctcgtcg
     1561 atgttgagcg ccatcacgcg cgggctcggc tcggccgcca ccaggcgcca cttgcgcccg
     1621 gccgggaagc cggcgccgcc caggccgcgc agccccgagt cctccatgat cttggtgagg
     1681 tcctcgcgcg tgcgcttgcc ggcgaggcac tcggcgatca gcttgtagcc gccggctttc
     1741 ttgtactgcg cgtagtcgat gtacttcggt tcctcgcagg tgagcttttt tgcctccact
     1801 gcggcgttga cggtctccac gctggcattg cccagcgcat tctggtgcac gacgacgcaa
     1861 ggcgccactt cacagcggcc gacgcacggc gccggaatga cccgcacgcc gggtccgaag
     1921 gaaagcttct tcagcagctc ctgcgcgccg gcgagctcgc acgaaatcga gtcgcatacc
     1981 cgcaccgtga tcgccgccgg cgcctcgccg ccctccttca ccacctcgaa gtggtggtag
     2041 aaggtggcga cctcgtagac ctcggtgagc gcgaggcgca tctcctgcgc cagcgcggcg
     2101 aggtgcgccg ctgtcagatg cccgtagcgg tcgttgatct tgtgcaggtg ctcgatcagc
     2161 aggtcgcgct ggcgcgattc gttgccgagt agcgcctgca cttcggagac cgctttcgga
     2221 tcgaccgggc ggcctttggg cgtcgaacga ggtttgcgca tg
//
LOCUS       DASUNT010000258         3780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1087075, whole genome shotgun sequence.
ACCESSION   DASUNT010000258 DASUNT010000000
VERSION     DASUNT010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087075"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1679)
                     /gene="groL"
                     /locus_tag="VFX94_05085"
     CDS             complement(72..1679)
                     /gene="groL"
                     /locus_tag="VFX94_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238928.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX5767592.1"
                     /translation="MSAKQLLFADTAHERLVKGMNILADAVRVTLGPKARTVVLEKSW
                     GAPTVINSGVIVAKEIELEDRFENMGAQMVREVAAKTSEVAGDGTTTATLLAAAIVNE
                     GMKYVAAGMNPMDLKRGIDLAVEKLIGELVRMAKPCSTRNEIAQVGAISANNDASIGN
                     MIAEAMEKVGREGVITVEDGKGLANELEVVEGTQFDRGFLSPYFITNAEKQTVVLEDA
                     YILLHDKKISTIRELLPLLEHVAKVGKPLLVIAEEVEGEALATLVVNKLRGVLHSCAV
                     KAPGFGDRRKAMLEDLAVLTGGRVVAEEAGISLEKAEPSVLGRARRVEIDKDDTTIIG
                     GAGEKQAIDARVAQIKREIEDATSDYDKEKLQERAAKLAGGVALIRVGAATETEMKER
                     KSRTEDALHATRAAVEEGVLPGGGVALLRARTCLSSLKGQNADQNAGIRIVERAVEEP
                     LRQIVRNAGEEPAVVVQRVLDGDGNFGYNAATGEYGDLVAMGVLDPRKVTRAALQNAA
                     SIASLILTTDCMIAQAPPAAPREEEMA"
     gene            complement(1676..2062)
                     /locus_tag="VFX94_05090"
     CDS             complement(1676..2062)
                     /locus_tag="VFX94_05090"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX5767593.1"
                     /translation="MTTIPQDVLLQFWKSQLDASLRLLETLTESAMRVYEAQLEAATE
                     AHADAEATRKAVLAAADTGQVLRLYTEWARANTEKSAAYCRSLMKALPTPAAPAGLDL
                     GMMDSAYKQWVEGVKRLYEGAVKPNA"
     gene            complement(2082..2321)
                     /locus_tag="VFX94_05095"
     CDS             complement(2082..2321)
                     /locus_tag="VFX94_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767594.1"
                     /translation="MGAPALELGRRAYAFATRPDIHGDAYVDPDTWAAQTPREHGSWW
                     PQWQRSLSERSGAPVEPPFPGRSRGAAFGHYVPEH"
     gene            complement(2302..2622)
                     /locus_tag="VFX94_05100"
     CDS             complement(2302..2622)
                     /locus_tag="VFX94_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767595.1"
                     /translation="MLSLEDCLALCELTEDEVLAIAHHEHLPEIAAAELGHYLCLTPD
                     GEMRIKSIFRDDVAEARAGGDRMRELALKLMLRNFILQHPSCEERHAQRLCAVERRDG
                     RACA"
     gene            complement(2615..2872)
                     /locus_tag="VFX94_05105"
     CDS             complement(2615..2872)
                     /locus_tag="VFX94_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767596.1"
                     /translation="MKPDTLQADIEHRIAELRGRHPRIAVVHATLDEWHEDRAPCHAL
                     RLDIRWAQHQSLVSGPVRPGGWAAVQAGFEAAAQKLEDNHA"
     gene            complement(2876..3385)
                     /locus_tag="VFX94_05110"
     CDS             complement(2876..3385)
                     /locus_tag="VFX94_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX5767597.1"
                     /translation="MKRLWVVGAALAAAASFALAQGPGYGYGPGSGPGYGRGMMDYGP
                     GYGPGGGYGPGMMGGGYGAGGTLAALNLTDEQRDKVFAIQEEHRKKNWAVMGEMRSEQ
                     FKLRNMYRGEKVDADKVVEQQKKVDGLRQQMLKSRVETHNQIAAVLTPEQRKQLKQYS
                     PGWLGDVEE"
     gene            3483..>3780
                     /locus_tag="VFX94_05115"
     CDS             3483..>3780
                     /locus_tag="VFX94_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiB"
                     /protein_id="HEX5767598.1"
                     /translation="MLRGLADTARDLGRMQEIGAVLLRYGFGDLVRRLGIVLHWKEAA
                     QSAPLNAPESARRALQDLGPTFVKLGQILATRVDLLPPEWITELEKLQDRAPASP"
BASE COUNT          521 a         1324 c         1226 g          709 t
ORIGIN      
        1 aatccttgaa gaagtcctcg aacgggtcgt agcgggtgat gttggccata aagccctcct
       61 ttcagtgtcg gtcaagccat ctcttcttcg cgcggcgcgg ccggcggcgc ctgcgcgatc
      121 atgcaatcgg tagtgaggat gaggctcgcg atcgaagcgg cattctgcag cgccgcgcgc
      181 gtcaccttgc gcggatcgag cacgcccatc gcaacgaggt cgccgtattc gccggtggcg
      241 gcgttgtagc cgaagttgcc gtcgccatcg agcacgcgct gcaccacgac cgcgggctcc
      301 tcgcccgcgt tgcgcacgat ctggcgcagg ggctcttcca ccgcgcgctc gacgatgcgg
      361 ataccggcgt tctggtcggc gttttgccct ttgaggctgc tcaagcaagt ccgtgcccgc
      421 agaagcgcga cgccgccgcc gggcaggacg ccttcctcga ccgccgcgcg cgtcgcgtgc
      481 agcgcgtcct cggtgcggct cttcctctcc ttcatctcgg tctcggtcgc cgccccgaca
      541 cggatcagcg ccacgccgcc ggcgagcttc gccgcgcgct cctgcagctt ttccttgtca
      601 taatcgctgg tcgcgtcctc gatctcgcgc ttgatctgcg cgacgcgcgc gtcgatcgcc
      661 tgcttctcgc ccgcgccgcc gatgatcgtg gtgtcgtcct tgtcgatctc gacgcgccgc
      721 gcgcgcccga gcaccgacgg ctcggccttc tcgagcgaga tcccggcctc ttcggccacc
      781 acgcggccgc cggtcagcac cgcgaggtcc tcgagcatcg ccttgcggcg gtcgccgaac
      841 ccgggcgcct tgaccgcaca cgagtgcagc acgccgcgca gcttgttcac cacgagcgtt
      901 gcgagcgcct cgccttccac ctcctcggcg atcacgagca gcggcttgcc caccttggcg
      961 acgtgctcga gaagcggcag gagctcacgg atcgtcgaaa tcttcttgtc gtggagcagg
     1021 atgtacgcgt cctcgagcac gaccgtctgc ttctccgcgt tggtgatgaa gtaaggggac
     1081 aggaagccgc ggtcgaactg ggtgccctcg acgacctcga gctcgttggc gagccccttg
     1141 ccgtcctcga ctgtgatcac gccttcgcgg ccgaccttct ccatcgcctc ggcgatcatg
     1201 ttgccgatcg aggcgtcgtt gttggcggag atggcgccga cctgggcgat ctcgttgcgc
     1261 gtcgagcacg gcttcgccat gcgcacgagc tcgccgatga gcttctcgac cgcgaggtcg
     1321 atgccgcgtt tgaggtccat cgggttcatt cccgccgcga cgtacttcat gccttcgttc
     1381 acgatcgccg cggcgagcag cgtcgcggtc gtggtgccgt cacctgcaac ctccgaggtc
     1441 ttggccgcta cctcgcgcac catctgcgcg cccatgttct cgaagcggtc ctcgagctcg
     1501 atttccttcg ccacgatgac gccggagttg atcacggtgg gcgcgcccca tgatttttcg
     1561 agaaccacgg tgcgcgcctt cggaccgagc gtgacgcgca ccgcgtcggc gagaatgttc
     1621 atgcccttca cgaggcgctc gtgcgccgta tcggcgaaca gcagctgctt ggcgctcatg
     1681 cgttcggctt tactgcgcct tcgtacaggc gcttcacgcc ctccacccac tgcttgtagg
     1741 cgctgtccat catgccgaga tcgagccctg cgggcgcggc cggcgtcggc agcgccttca
     1801 tcagcgagcg gcagtacgca gccgacttct ccgtgttggc gcgcgcccac tcggtgtaga
     1861 gccgcagcac ctgcccggtg tcggccgccg ccaggacggc cttgcgggtc gcctcggcgt
     1921 cggcgtgtgc ctcggtcgcc gcttcgagct gcgcttcata gacgcgcatt gcgctttccg
     1981 tcagcgtctc gaggagccga aggctcgcgt cgagctggct tttccagaac tgcagcagaa
     2041 cgtcttgcgg gatggtcgtc atgacggttc ctccttttgt ctcagtgctc agggacgtag
     2101 tggccgaacg cggcgccgcg cgagcggccg gggaaaggag gctcgactgg cgcgccggag
     2161 cgctcgctga gcgagcgttg ccactggggc caccacgagc cgtgctcgcg cggggtttgt
     2221 gcggcccacg tatcggggtc gacataggcg tctccatgga tgtcagggcg cgtggcgaat
     2281 gcgtaggctc gccggccgag ctcaagcgca ggcgcgccca tccctgcgct ccaccgcgca
     2341 aagtcgctgc gcgtggcgct cctcgcagct cgggtgctgg aggatgaagt tacgcagcat
     2401 gagcttcagc gcgagctcgc gcatgcggtc gccgccggcg cgcgcctcgg caacgtcgtc
     2461 gcggaagatg gacttgatgc gcatctcgcc atccggcgtg aggcacaggt agtggccgag
     2521 ctccgcggcg gcgatctcgg gcaggtgctc gtggtgcgcg atcgcgagga cttcgtcttc
     2581 ggtcagctcg cacagtgcaa ggcagtcttc caggctaagc atggttgtcc tccagtttct
     2641 gcgccgcagc ctcgaagccg gcctgcaccg ccgcccagcc gcccggccgc accgggccgc
     2701 tcaccagact ttggtgctgc gcccagcgga tgtcgagccg cagcgcgtgg cacggcgcgc
     2761 ggtcctcgtg ccactcgtcg agcgtcgcat gcacgaccgc gatccgcgga tggcggccgc
     2821 gcagctcggc gatgcggtgc tcgatgtcgg cctgcagcgt gtcgggcttc atggactatt
     2881 cctcgacatc gccgagccac ccgggcgaat actgcttgag ctgcttgcgc tgctcgggcg
     2941 tgagcacggc cgcgatctgg ttgtgcgtct ccacgcggga tttgagcatc tgctggcgca
     3001 gcccatccac cttcttctgc tgctccacga ccttgtccgc gtccaccttc tcgccgcggt
     3061 acatgttgcg cagcttgaat tgctcggagc gcatctcgcc catcaccgcc cagttcttct
     3121 tgcggtgctc ttcctggatc gcgaatacct tgtcgcgctg ctcgtcggtg aggttgagcg
     3181 ccgccagcgt tccgccggcg ccgtagccgc cgcccatcat cccggggccg taaccgccgc
     3241 cgggaccgta gccgggaccg taatccatca ttccgcgacc gtagcccggg ccagatcctg
     3301 gcccgtagcc gtaacccggg ccttgcgcca atgcaaaaga ggcggccgcg gcaagcgccg
     3361 cgccgaccac ccataaccgt ttcatggaca tctcctttcg agttgagcgt acctccggcg
     3421 ctccggagct ttttgatcgg ggtcaaggcg ggtcaactcc accttcctac gatgaaaaac
     3481 aaatgctgcg aggcttggcg gacacggcgc gggacctcgg ccggatgcag gaaatcggcg
     3541 cggtcctgct gcgctacggc ttcggcgacc tggtgcggcg cctcggcatc gtcctgcact
     3601 ggaaggaggc ggcgcagtcg gcgccgctca acgctccgga aagcgcacgc cgtgcgttgc
     3661 aggacctcgg cccgacgttc gtcaagctcg gacagattct cgcgacccgg gtcgaccttc
     3721 tcccgccgga atggatcacg gagctcgaga agcttcagga tcgggcgccg gcgtccccgt
//
LOCUS       DASUNT010000259         4905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1087176, whole genome shotgun sequence.
ACCESSION   DASUNT010000259 DASUNT010000000
VERSION     DASUNT010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087176"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VFX94_05120"
     CDS             <1..326
                     /locus_tag="VFX94_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX5767599.1"
                     /translation="TSTLFGAGGPPYAIYLSQRGLTKAQFRATLGFATLTSISLRLIA
                     FVLTGLLLDRDVWLCALAVVPACLLGIWLARKMYLRISRDALLRAVAVLLLFSGASLI
                     SRAVL"
     gene            complement(304..642)
                     /locus_tag="VFX94_05125"
     CDS             complement(304..642)
                     /locus_tag="VFX94_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5767600.1"
                     /translation="MPGFTVRMTIERDALEVYNFVLDAKNLPRWASGIDAAAKVRFVE
                     RNKYGVLDHYVTVRSREVYVPMRVFANGEGAEVLLTVFRQPGMSEQKFLEDTQWVRRD
                     LEALKALLEK"
     gene            complement(648..2165)
                     /locus_tag="VFX94_05130"
     CDS             complement(648..2165)
                     /locus_tag="VFX94_05130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5767601.1"
                     /translation="MRRHQSVWAASAELPQFSPLTENVHVDVCIVGAGISGLTTAYLL
                     ARAGKAVAVLDDGPVAGGMTQMTTAHITHQLDDRYFELEKLHGREAARLAAESHTAAI
                     DRIETIVRQENIDCDFTRLDGYLFLAEGDTRETLERELDAAHRAGLSAVELEGRAPYA
                     SFDTGPCLRFPNQAQFHPLKYLARLARAIVHEGGCIFTGSHADRIEGGVPALVHADRH
                     VVTADAVVVATNTPVNDRVAIHTKQAPYMTYVIGARIPRDSVARVLAWDTGDPYHYIR
                     TYQDYLIVGGEDHKTGQASDTPARYARLEAWARARFPQMGEVELAWGGQVMETQDYLA
                     FIGRNPMDHESVFVVTGDSGMGITHGTIAGMLIPDLIAGRPNPWEALYDPSRVRLRAA
                     GDFARENSNVALQYADYLTGGDVASVEEIAAGTGAIVRQGLEKHAVFRDENGTLHACE
                     AKCPHLGCLVHWNPAETTWDCPCHGSRFDPYGKVINGPANRDLAPAERFREQRAA"
     gene            complement(2215..3567)
                     /gene="gorA"
                     /locus_tag="VFX94_05135"
     CDS             complement(2215..3567)
                     /gene="gorA"
                     /locus_tag="VFX94_05135"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006408013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEX5767602.1"
                     /translation="MDFDYDLFTIGGGSGGVRASRVSASYGAKVAIAEPGRFGGTCVN
                     VGCIPKKLFSYAAHWREDFHLAAAYGWTVGEPRFDWTTLLANKDREIARLNGVYERVL
                     VNAGVEIHRSRATVTGPNEVEVGGKRVRARHILVATGSWPVLPAIPGIEHAITSNEAF
                     HLEGLPRRAVVVGGGYIALEFASIFHGLGVGTTLAYRGKRLLRGFDAELGTRIAEEMS
                     AKGVAIRFGCEPAALRRNGGGEIEIEFTDGSIERTDLVMFATGRRPNTAKLGLEAAGV
                     RLAADGAVIVDNYSSSSVPSIHAVGDVTNRINLTPVATAEAMWLARTLFRGEPTAVDH
                     GNVPTAVFANPNLASVGLSEEKARQRFGAIDLYKASFRALKLTMTESKERTFMKLVVD
                     RASQRVVGAHMIGAHAGEIIQGIAIAVKLGATKAVFDATIGIHPTVAEEFVTMREKAS
                     "
     gene            complement(3569..3838)
                     /locus_tag="VFX94_05140"
     CDS             complement(3569..3838)
                     /locus_tag="VFX94_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767603.1"
                     /translation="MAPDEERTLKLATDLDRKAIEARLVQVRREARSANLKDLADLFN
                     GVEGMPRALIERNVKNALAWLADKPQLSRIASQLELVGLNLQNLK"
     gene            complement(3849..>4905)
                     /locus_tag="VFX94_05145"
     CDS             complement(3849..>4905)
                     /locus_tag="VFX94_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5767604.1"
                     /translation="ERCARGEIELVHATQVSLKDLARFSEPAAALQYVRALPEVEENR
                     ACWAQGRDGPKIFRRGDAPYFEIHWSDPDETGETTYDIIPGERKRLDRWVERITAPNP
                     GMMTGPGTNSYIVGGVALIDPGPAIDSHIEKIMAETKGGLRWIFCTHTHLDHSPAAAR
                     LKARTGATVLGRPAPPGQDSTFEPDVVLESGQRIELGEVSLRAIHTPGHASNHVCYLL
                     EQTRMLFTGDHVMQGSTVVINPPDGDMRVYLESLERLLGEDIAIIAPGHGYLIGAPHR
                     ELRRLIAHRLNRERKVIAALERLGHPSLDDMLPLVYDDVPERAHRVAARSLTAHLEKL
                     VADGAVRARDGGYTLVR"
BASE COUNT          727 a         1648 c         1669 g          861 t
ORIGIN      
        1 ttacgagcac gctcttcggc gccggcgggc cgccctacgc catctacctc tcgcagcgcg
       61 ggcttaccaa ggcgcagttc cgcgcgacac tgggcttcgc cactctcacc agcatctcgt
      121 tgcggctcat cgcttttgtg ctgaccgggc tattgctcga ccgcgacgtg tggctgtgcg
      181 cgcttgccgt ggttcctgcc tgcctgctcg gcatctggct cgcgcgcaag atgtacctgc
      241 gcatttcgcg cgacgcgctg ctgcgcgccg tcgcagtgct tctacttttc tccggggcgt
      301 ctcttatttc tcgagcagtg ctttgagcgc ttcgaggtcg cgccgcaccc actgcgtgtc
      361 ttcgaggaac ttctgctcgc tcatgccggg ctggcggaag accgtgagca gcacttccgc
      421 cccctcgccg ttggcgaaga cccgcatcgg cacgtagacc tcgcgcgagc gcacggtcac
      481 gtagtggtcg agaacgccgt acttgttgcg ctcgacgaag cgcaccttgg ccgcggcgtc
      541 gatgccggag gcccacctgg ggaggttttt cgcgtcgagc acgaagttgt agacctcgag
      601 cgcatcgcgc tcgatcgtca tgcgtacggt gaagccaggc aacgtcttca ggcggcgcgc
      661 tgctcccgga agcgctcggc cggcgcgaga tcgcggtttg ccggcccgtt gatcacctta
      721 ccatagggat cgaagcgcga gccgtggcag ggacagtccc aagttgtttc cgcggggttc
      781 cagtgcacga ggcagccgag gtgcggacat ttcgcctcgc atgcgtgcag cgtgccgttt
      841 tcatcgcgga agacggcgtg cttctccagg ccctggcgca cgatggcgcc ggttccggca
      901 gcgatttcct ccacggaggc gacgtcgccg cccgtcagat agtcggcgta ttggagcgcc
      961 acgttggagt tctcgcgcgc gaagtcgcct gcggcgcgca gccggacgcg cgaagggtca
     1021 tacagcgctt cccatgggtt cggccggccg gcgatgagat ccgggatcag catgccggcg
     1081 atcgtgccgt gcgtgatccc catgcccgaa tcgccggtga ccacgaacac gctttcgtgg
     1141 tccatcggat tgcgaccgat gaaggcgagg tagtcttgcg tctccatcac ctgacctccc
     1201 cacgcgagct cgacttcgcc catctgcgga aagcgcgcgc gcgcccaggc ctcgaggcgc
     1261 gcgtagcgcg cgggcgtgtc gctcgcctga ccggtcttgt ggtcctcgcc gccgacgatg
     1321 agatagtcct ggtaggtccg gatgtagtga tacgggtcgc cggtgtccca ggcaagcacg
     1381 cgcgcgacgg aatcgcgcgg tatgcgcgcg ccgatcacgt acgtcatgta gggcgcctgc
     1441 ttggtgtgga tggcgacccg gtcgttgacc ggcgtattgg tcgcaaccac gacggcatcg
     1501 gcggtgacca cgtgcctgtc cgcatgcacg agcgccggga ccccgccttc gatgcgatcg
     1561 gcgtgcgagc cggtgaagat gcacccgccc tcgtgcacga tcgcccgcgc aagccgcgcg
     1621 aggtacttga gcgggtggaa ctgcgcctgg ttcggaaagc gcaggcacgg gccggtatcg
     1681 aaggaggcgt aaggcgcacg cccctcgagc tcgaccgccg acagccccgc ccgatgcgcc
     1741 gcgtcgagct cgcgctcgag cgtctcccgc gtgtccccct cggcgagaaa aaggtagccg
     1801 tcgagccgcg tgaagtcgca gtcgatattc tcctgccgga cgatggtctc gatgcgatcg
     1861 atggccgccg tgtggctttc ggcggcgagc cgcgccgcct cgcgcccgtg gagcttctcg
     1921 agctcgaagt agcggtcgtc gagctggtga gtgatgtgcg cggtcgtcat ctgggtcatg
     1981 ccgcccgcga ccgggccgtc gtccagcacc gcgaccgcct tgccagcgcg cgcgaggaga
     2041 taagccgtcg taagccccga gatcccggcg ccgacgatgc acacgtcgac gtgcacgttc
     2101 tccgtgagcg ggctgaactg cggcagctcg gcgcttgccg cccacaccga ttgatggcgt
     2161 cgcatgatga cgccagcgag caagaaacgc acccaaaaag ggggtcagga ccctttagga
     2221 cgccttctcg cgcatggtca cgaactcttc cgcgacggtc ggatggatgc cgatggtggc
     2281 gtcgaacacg gccttggtgg cgccgagctt caccgcgatg gcgattccct ggatgatctc
     2341 gcccgcatgc gcgccgatca tgtgcgcgcc aaccacccgc tggcttgcgc gatcgaccac
     2401 cagcttcatg aacgtgcgtt ccttgctctc ggtcatggtg agcttgagcg cgcgaaacga
     2461 cgccttgtag aggtcgatgg cgccgaagcg ctggcgcgcc ttctcctccg agagtccgac
     2521 gctcgccagg ttggggttcg cgaacaccgc ggtcggaacg ttcccgtggt ctaccgccgt
     2581 cggctcgccg cgaaacagcg tgcgcgcaag ccacatcgcc tccgcggtcg cgaccggggt
     2641 gaggttgatg cggttggtca cgtcgccgac ggcatggatg gagggcacgc tcgagctcga
     2701 gtagttgtcc acgatcaccg cgccatcggc ggcgaggcgc actcccgccg cctccaggcc
     2761 gagctttgcc gtattcggcc gccggccggt ggcgaacatc acgaggtcgg tgcgctcgat
     2821 cgagccgtcg gtgaactcga tctcgatctc gccgccgcca ttgcggcgca aggccgccgg
     2881 ctcgcagccg aagcggatcg cgacgccttt ggccgacatc tcctcggcga tgcgcgtgcc
     2941 gagttccgca tcgaagccgc gcagcaggcg cttgccccga taagcgagcg tcgtcccgac
     3001 gcccaggccg tggaagatcg aggcgaactc gagcgcgatg tagccgccgc cgaccacgac
     3061 cgcgcgccgc ggcaggccct cgaggtggaa tgcttcgttc gacgtgatcg cgtgctcgat
     3121 gcccgggatc gcgggaagca ccggccacga gcccgtagcg acgaggatat gtcgggccct
     3181 cacccgcttt ccgccgacct cgacctcatt tggcccggtt accgtggcgc gggagcggtg
     3241 gatctccact ccggcgttga cgaggacgcg ctcgtatacg ccattcagtc gggcgatctc
     3301 gcggtccttg ttggcgagga gcgtggtcca gtcgaagcgc ggctcgccca ccgtccagcc
     3361 gtaggcggcg gcgaggtgga aatcctcgcg ccagtgcgcg gcgtaggaaa agagcttttt
     3421 cgggatgcag ccgacgttga cgcaagtgcc gccgaagcgc ccgggctcgg cgatcgcgac
     3481 tttcgccccg tacgaggcgg acacgcggct cgcgcgcact ccgcccgagc cgccgcctat
     3541 cgtgaagagg tcgtaatcga aatccaggct acttcaggtt ctgaaggttg agcccgacga
     3601 gctcgagctg cgaggcgatg cggctgagct gcggcttgtc ggcgagccac gcgagcgcgt
     3661 tcttcacgtt gcgctcgatc agcgcgcgcg gcatgccctc gacgccgttg aagagatcgg
     3721 cgaggtcctt gaggttggcc gatcgagctt ccctgcgcac ctggacaagc cgcgcctcga
     3781 tcgccttgcg atcgagatcg gtggcgagct tgagggttct ttcttcgtcg ggcgccatgc
     3841 ctgcgcgctc atcttaccag cgtgtaaccg ccgtcccgcg cgcgcacggc gccgtcggcg
     3901 accagctttt cgaggtgcgc ggtgagcgag cgtgccgcaa cgcggtgcgc gcgctcgggc
     3961 acatcgtcgt acacgagcgg cagcatgtcg tcgagcgacg gatgcccgag gcgctcgagc
     4021 gccgcgatga ccttgcgctc gcggttgaga cgatgcgcga tgagccggcg cagctccctg
     4081 tgcggcgcgc cgatgaggta gccatgcccc ggcgcgatga tggcgatatc ttcgccgagc
     4141 agccgctcaa gcgactcgag gtacacgcgc atgtcgccgt cgggcggatt gatcaccacg
     4201 gtcgagccct gcatcacgtg gtcgccggtg aaaagcatcc gggtctgctc gagcaggtag
     4261 cacacatggt tcgaggcgtg gcccggcgtg tggatcgcgc gcagcgatac ttcgccgagc
     4321 tcgatgcgct gcccgctctc caagacgaca tcgggctcga acgtgctgtc ctgccccggc
     4381 ggcgccggcc ggccgagcac cgtggcgccg gtcctggcct tgagccgcgc ggccgccggc
     4441 gaatggtcga ggtgggtgtg cgtacagaag atccagcgca gcccgccttt ggtttccgcc
     4501 atgattttct cgatgtgcga gtcgatcgct ggtccgggat cgatgagcgc cacgccgccg
     4561 acgatgtagc tgttggtgcc cggcccggtc atcattccgg ggttgggcgc ggtgatgcgc
     4621 tcgacccagc gatcgaggcg cttgcgttcg ccggggatga tgtcgtaggt cgtctcgccg
     4681 gtctcgtcgg gatcggacca gtggatctcg aagtagggcg cgtcgccgcg gcggaaaatc
     4741 ttcggcccgt cccttccctg cgcccagcag gcgcggttct cctccacctc gggcagcgcg
     4801 cgcacatact gcaacgccgc ggccggctcg gaaaagcgcg caaggtcctt cagcgacacc
     4861 tgcgtcgcat ggacgagctc gatctcgccc cgcgcgcagc gctcg
//
LOCUS       DASUNT010000260         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1263390, whole genome shotgun sequence.
ACCESSION   DASUNT010000260 DASUNT010000000
VERSION     DASUNT010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263390"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /gene="dnaX"
                     /locus_tag="VFX94_05150"
     CDS             complement(<1..1354)
                     /gene="dnaX"
                     /locus_tag="VFX94_05150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX5767605.1"
                     /translation="MSYQVLARKWRPRDFGSLVGQEHVVRALRHALEQKRLHHAYLFT
                     GTRGVGKTTLARILAKCLNCETGITPQPCNTCSACAEIDAGRFVDLLEVDAATNTKVD
                     EMRQLLDTAQYAPTRGRFKVYVIDEVHQLSGHAFNAMLKTLEEPPEHMKFILATTDPQ
                     KIPVTVLSRCLQFNLKQMPREAIAEHLGKILGREGIQYEPGALALLARAAAGSMRDAL
                     SLLDQAIGHGGGAVTAASAAEMLGAIDQGHLVRLIEAIAADDAAGAVKIADDMQARSL
                     SFDGALAGLASLLFQVALAQSVAGAVDDAHVKELASRLDPESVQLYYQIALQGREDLP
                     LAPDEHGGFVMTVLRMLAFRPEGAGRDLPVPKKTENVQARPQSSDWTALVQQLPVTGA
                     ARELARNAELRRRDGASFELVVPKAKAYLAERSYVEKLKAALEQQLAAPVSLKVSVGE
                     V"
     gene            complement(1371..1469)
                     /gene="ffs"
                     /locus_tag="VFX94_05155"
     ncRNA           complement(1371..1469)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFX94_05155"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1656..2075
                     /locus_tag="VFX94_05160"
     CDS             1656..2075
                     /locus_tag="VFX94_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767606.1"
                     /translation="MRLALTVAASLLAAALLAACHRAPTRDVFIPPYAEKGCWAQFYE
                     QAQFGAPVLQVDGPTFVEAIPGSIVMVPNLDKIPPQPLFENARSLVVGPNARLVGYAE
                     TLFRGTPTVLDPGTKVPDAASVGYPQRFLSLTLECKA"
     gene            complement(2086..2259)
                     /locus_tag="VFX94_05165"
     CDS             complement(2086..2259)
                     /locus_tag="VFX94_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEX5767607.1"
                     /translation="MLKWAIIFFVISVVAGILGFTGVASTTRTIAKVLFFIALAIFLI
                     VLIFGVMLGMLVF"
     gene            complement(2303..>2542)
                     /locus_tag="VFX94_05170"
     CDS             complement(2303..>2542)
                     /locus_tag="VFX94_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5767608.1"
                     /translation="EATDRVDLLEEASIEHNAAKQLMAELESNGDGVHRRALVKVLGE
                     YVGHHIREEEEKIFPLVEKMGVDLQALGEELAERR"
BASE COUNT          419 a          881 c          784 g          458 t
ORIGIN      
        1 cgacttcccc gaccgagacc ttcaggctca ccggcgctgc gagctgctgc tctagcgccg
       61 ccttgagctt ttccacgtag ctgcgctcgg cgaggtaggc cttggctttc ggcaccacga
      121 gctcgaagct cgcaccgtcg cgacgcctta attcggcgtt acgcgcaagc tctcgcgcgg
      181 cccccgtgac cggcaactgc tgcacgagcg ccgtccagtc gctgctttgt ggacgagcct
      241 ggacattttc agttttcttc gggaccggca agtccctgcc ggcgccttcg ggacggaacg
      301 caagcatccg cagcaccgtc atgacgaagc cgccgtgctc gtcgggcgcg agcggcaggt
      361 cttccctgcc ctgaagcgcg atctgatagt agagctgcac cgactccgga tcgagccggc
      421 ttgcgagctc cttcacgtgc gcgtcatcca ccgcgccggc gaccgactgc gccagtgcga
      481 cttgaaaaag caggctggcc agcccggcca gcgcgccgtc gaaggacagg ctgcgcgcct
      541 gcatgtcgtc ggcgatcttc accgcgccgg cggcatcgtc cgcggcgatc gcctcgatga
      601 gccgcacgag atgaccctgg tcgatggcgc cgagcatttc tgcggcgctc gccgccgtga
      661 ccgcgccgcc gccatgcccg atcgcctggt cgagcagcga cagcgcatcg cgcatgctgc
      721 ccgccgcggc gcgcgcgagt agcgcgagcg ctcccggctc atactgaatg ccctcccgac
      781 ccagaatctt ccccaggtgc tcggcaatgg cttcgcgcgg catctgcttg aggttgaact
      841 gcagacagcg gctgagcacc gtcaccggga tcttctgagg atcggtagtc gccaggatga
      901 acttcatgtg ctcgggcggc tcctccagcg tcttcagcat ggcgttgaag gcgtggcccg
      961 agagctggtg cacctcgtcg atcacgtaga ccttgaagcg gccgcgcgtc ggcgcgtact
     1021 gcgcggtgtc cagcagctgg cgcatctcgt cgaccttggt gttcgtcgcc gcgtcgacct
     1081 cgagcagatc gacgaagcga ccggcgtcga tctcggcgca cgccgagcac gtgttgcacg
     1141 gctggggcgt gatcccggtc tcgcagttca ggcacttggc gaggatgcgc gcgagcgtgg
     1201 tcttgccgac gccgcgcgtg ccggtaaaga gatacgcgtg gtgcaggcgc ttctgctcga
     1261 gcgcgtggcg cagggcgcgc accacgtgct cctgccccac aaggctgccg aaatcccgcg
     1321 gccgccattt gcgggcaagg acttgatagc tcatcgctcg actagaattc ggtggcgagc
     1381 ccgacccccg gcactcgcag cgaccggctg tggctgcttc cttccggacc tgaccagttt
     1441 caccagactg caatgcgggg aggcccgccg gcatcaattc taatttgaaa cggccaggac
     1501 agtaagcccg ctgtcagctt gaagtcagcg actcgttgga agccggcgtt actctgtgca
     1561 cgtatcctcc tccatcgcaa tagtcgcgat ggcttcgacg cccgcgggcg caagctccgc
     1621 gggcgttttt cttggtgcat gcgagaatag cgcgcatgcg cttagcgctc accgtggccg
     1681 caagcttgct tgctgcggcc ctgctcgccg cctgccaccg cgccccaacc cgcgacgtat
     1741 tcattccgcc gtacgcggag aagggctgct gggcacagtt ctacgagcag gcgcaattcg
     1801 gtgcgcccgt cctccaggta gacggtccca ccttcgtcga ggccatcccc ggctcgatcg
     1861 tcatggtccc gaacctggac aagatcccgc cgcagccgct gttcgagaac gcgcgcagcc
     1921 tggtcgtggg cccgaacgcc aggctcgtcg gctatgccga gaccctcttc cgaggcactc
     1981 cgacggtact cgatccggga acaaaggtgc ccgacgccgc ttcagtcgga tacccgcagc
     2041 ggttcctctc gctgacgctt gaatgcaagg cctgagcgct cgccgctaga aaaccagcat
     2101 cccgagcatc acgccgaaga tcaggacgat caggaagatc gcgagggcga tgaaaaaaag
     2161 caccttggcg atcgtgcggg ttgtggaagc caccccggtg aagccgagaa ttcccgccac
     2221 caccgaaatg acgaaaaaga tgatcgccca cttcagcatg agtcggtgcc gctcgctccg
     2281 cgcgtcccgt acattgcttt gactacctgc gctcggcgag ctcctcgccc agcgcttgca
     2341 aatcgacgcc catcttctcg accagcggga agatcttctc ctcctcctcg cggatgtggt
     2401 ggccgacgta ctcgccgagc accttcacca gcgcgcgccg gtgcacaccg tcgccgttcg
     2461 actcgagctc ggccatgagc tgcttcgccg cgttgtgctc gatggaagcc tcttccagca
     2521 gatctacgcg atcggttgct tc
//
LOCUS       DASUNT010000261         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_35064, whole genome shotgun sequence.
ACCESSION   DASUNT010000261 DASUNT010000000
VERSION     DASUNT010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35064"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VFX94_05175"
     CDS             <1..393
                     /locus_tag="VFX94_05175"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HEX5767609.1"
                     /translation="RVAKKLSAAGFEDSDISEALHWLAGMAQTSGGVREPLPDARSSF
                     RAYAPRELAKLDAGCRGFLLTLEHSGVLSAETRELVLERALAAAGNTLNLEQLKLVVL
                     MVLWNQQTPTSRLVAEQLFSAPHARLAS"
     gene            482..>2650
                     /locus_tag="VFX94_05180"
     CDS             482..>2650
                     /locus_tag="VFX94_05180"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEX5767610.1"
                     /translation="MAKKLIIAEKPSVAADIARALGGFKRSGDYYESDAYVISSAVGH
                     LLELAVPEEHDVKRGKWSFSRLPVIPQQFTLAPIEKSESRLKLLLRLMKRKDVTGLVN
                     ACDAGREGELIFRYIVQHARNKKPIERLWLQSMTPQAIRDGFERLRRDKEMLPLADAA
                     KSRSEADWLVGINGTRAMTAFNSKEGGFYLTTVGRVQTPTLAILVEREERIRAFTARP
                     YWEVHARFAAQTGEYPGRWFDANFKKDDDGERKPERLWSADEAEAIVAACLGKTGIAS
                     EETRPSTQLSPLLYDLTSLQREANARFGFPARMTLSLAQSLYERHKVLTYPRTDARAL
                     PEDYLDTVKKTLEQLSEAREFLVFAKQILKQGWVKPNKRVFDDSKISDHFAIIPTLQS
                     PRNLNEAEQKLYDMVARRFLAVFYPAAEYLQTTRITRVGEHHFRTEGKVLQAPGWLAV
                     YGRAGNEESENLPAIVQGEKVTVRGIAPHANQTKAPPRYTEATLLSAMEGAGKLVEDD
                     ELRAAMEAKGLGTPATRAAIIEGLIREDYVHREGRELVPTPKAFSLMFALHMLHIVEL
                     SSPELTGEWEHKLKLIEAGKLTREEFMRGINDLVRNVVGVIKNGEIPDVAYATVPAPC
                     PKCGGTVQENYRKVQCQKCDFTLWRVISGREWAPEELAELFTKRFVGPLTGFRSKQGR
                     PFSAGMRLNDEMRLEFDFGQGSADGDEAPDFSGQEPLGACP"
BASE COUNT          511 a          849 c          862 g          428 t
ORIGIN      
        1 cgcgtggcga agaagctgtc ggccgccggg ttcgaggact ccgacatcag cgaggcgctg
       61 cactggctgg ccgggatggc gcagacatct ggcggcgtgc gcgagccatt gcccgacgcg
      121 cgctcgagct tccgtgcata cgcgccgcgc gagctggcaa agctcgacgc cgggtgccgc
      181 ggcttcttgc ttaccctgga gcattcgggc gttctgagcg cggaaacgcg cgagctcgta
      241 ctcgaacgcg cgctcgccgc cgcgggcaat acgctgaacc ttgaacagct caagcttgtc
      301 gtgctgatgg tgctctggaa ccagcaaacg cccaccagcc ggctggtggc ggagcagctc
      361 ttttccgcgc cacacgctcg cctcgcgagc tagccttttc gctcggcatt ttctcacttc
      421 ggcgccgggt ggttgaagcg cgcgcggctt tgcacttatc atctgcggcc tttcacgccg
      481 catggccaag aaactcatca tcgccgagaa accgtcggtc gcggccgaca tcgctcgcgc
      541 gctcggcggc tttaaacgca gcggggatta ttacgagagc gatgcttacg taatttcctc
      601 ggcggtgggg catttgctcg agcttgcagt gcccgaagag catgacgtga agcgcggcaa
      661 gtggtcgttc agccgtctgc ccgtcattcc gcagcagttc acgctcgccc cgatcgaaaa
      721 atccgaaagc cgcctcaagc tgctgctacg gctcatgaag cgcaaggacg tcaccggcct
      781 ggtgaacgca tgcgatgccg ggcgcgaagg cgagcttatc ttccgctaca tcgtgcagca
      841 cgcgcgcaac aagaagccca tcgagcggct gtggctgcaa tccatgacgc cgcaagcaat
      901 ccgcgacggc ttcgagcggc tgcgccggga caaggagatg ctgccgctgg cggacgcggc
      961 gaagagccgc tcggaagcgg actggctggt aggcatcaac gggacccgcg ccatgacggc
     1021 cttcaattcc aaagaaggcg gtttctacct gacgacggtg gggcgcgtgc agactccgac
     1081 gctcgccatc ctggtcgagc gcgaggagcg aatccgcgcg tttaccgcac gcccctactg
     1141 ggaagtccac gcgcgcttcg ccgcccagac aggtgaatat ccgggacgct ggttcgatgc
     1201 gaactttaag aaggacgatg atggcgagcg caagcccgag cggctgtgga gtgcagacga
     1261 ggccgaagcc atcgtcgccg cgtgcctcgg caagacgggc atcgcctccg aagagacccg
     1321 gccttcaacg cagctttcgc cgctgctcta cgaccttacc agcctgcagc gcgaagcgaa
     1381 cgcgcgcttc ggcttcccgg cgcgcatgac cttatcgctt gcgcagtcgc tttacgagcg
     1441 ccacaaggtg ctcacttatc cgcgtaccga cgcccgcgcg ctacccgagg attacctcga
     1501 cacagtaaag aagacgctgg aacagctgag cgaagcgcgc gaatttcttg tgttcgcgaa
     1561 gcagatcctg aagcagggat gggtcaagcc gaacaagcgc gtcttcgatg acagcaagat
     1621 ctccgaccac ttcgcgatca ttccaaccct ccagagcccg cgtaacctga acgaggccga
     1681 gcagaaactg tacgacatgg tggcgaggcg ctttcttgcc gttttctatc ccgccgccga
     1741 gtacctccag accacgcgca tcactcgcgt gggggagcat cacttcagga ccgaaggcaa
     1801 agtgctgcag gcgccgggct ggcttgccgt ctacggacgc gcgggtaatg aagaaagcga
     1861 gaacttgccg gccatcgtcc agggcgagaa ggtcacggtg cgcgggattg ccccgcacgc
     1921 gaaccagacc aaggcgccgc cgcgatacac cgaggcgacg ctgctctcgg cgatggaggg
     1981 cgcaggcaag ctggtggaag acgacgagct gcgggcggcc atggaagcca agggcctcgg
     2041 cacgccggcg acgcgcgccg cgatcatcga aggcctgatc cgcgaagact acgtgcatcg
     2101 cgaggggcgc gagctcgtgc cgacgccgaa ggcgttctcg ctaatgttcg cgctgcatat
     2161 gcttcacatc gtggagctca gctctcccga gctcaccggc gaatgggagc acaagctcaa
     2221 gctgatcgag gccggcaagc tcacgcgaga agaattcatg cgcggcatca acgacctggt
     2281 gaggaacgtg gtcggggtga taaagaacgg cgagattccg gacgtcgcct acgccaccgt
     2341 gcccgcgccc tgcccgaagt gcggcggcac ggtgcaggag aactatcgaa aggtgcaatg
     2401 ccagaagtgc gacttcaccc tgtggcgcgt gatctcgggc cgcgaatggg cgccggagga
     2461 gctggccgag ctcttcacca agcgcttcgt cgggcccctg accgggtttc gcagcaagca
     2521 ggggcgcccg ttcagcgccg gcatgcgcct caatgacgag atgcgcctcg agttcgactt
     2581 cggccagggg tccgcggacg gcgacgaagc gcccgacttt tcaggacagg agccgctcgg
     2641 cgcgtgcccg
//
LOCUS       DASUNT010000262         2306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_736625, whole genome shotgun sequence.
ACCESSION   DASUNT010000262 DASUNT010000000
VERSION     DASUNT010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736625"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /gene="aceA"
                     /locus_tag="VFX94_05185"
     CDS             complement(<1..1174)
                     /gene="aceA"
                     /locus_tag="VFX94_05185"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEX5767611.1"
                     /translation="MIEATKKDWAENPRWKDVKRGYGAEDVVRLRGSVQIEHTLARLG
                     AEKLWKLVNARPYVNSLGALTGNQAVQQVRAGVPAIYLSGWQVAADANDSLHMYPDQS
                     LYAVSSVPTVVRRINNALLRADQIQTMEAQGGKATAPDIDWLAPIVADAESGFGGVLN
                     AFELMKSMIEAGAAGVHYEDQLASVKKCGHMGGKVLVPTQEAVQKLIAARLAADIMGV
                     PTVLLARTDAEAADIVTSDIDENDKPFITGERTSEGFYKTRKGFDQALSRGLAYAEYA
                     DMVWCETGTPDLTFAKRFAEGIRKKYPEKMLAYNCSPSFNWKRNLDDATIARFQRELG
                     AMGYKFQFITLAGFHSLNYSMFELAHGYARTGMSAFVELQQKEFAASEKGFTAVKHQ"
     gene            complement(1357..>2306)
                     /gene="rodA"
                     /locus_tag="VFX94_05190"
     CDS             complement(1357..>2306)
                     /gene="rodA"
                     /locus_tag="VFX94_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX5767612.1"
                     /translation="LCVALVAMWIAAQISPQMLMRFAVPAYLLGLALLVAVALFGDVV
                     NGARRWLHVGVTRFQPSEMMKLALPLMLAWYFHRRESTLRLRDFAFAGLLVAMPVGLI
                     VRQPDLGTAALVGAAGFYVIFFAGVSWRLLGSLTALFLVALFPLWGVLHDYQRKRVLT
                     LLDPTQDPLGAGYHIIQSTIAVGSGGVSGKGWLNGTQAHLEFIPERHTDFIFAVYSEE
                     FGLIGNIVLLVIYALLIGRGLVIAANAATLFARLLAGAVTLMFFTYAFVNMGMVSGIL
                     PVVGVPLPFLSYGGTALLTLFIGVGILMSVQRHRTLVQR"
BASE COUNT          422 a          734 c          754 g          396 t
ORIGIN      
        1 gctggtgctt gacggcggtg aatcccttct cggaggccgc gaactccttc tgctgcagct
       61 cgacgaaggc gctcatgccg gtcctcgcgt agccgtgcgc aagctcgaac atcgagtagt
      121 tgagcgaatg gaagccggcg agcgtgatga actggaactt gtagcccatc gcgccgagct
      181 cgcgctggaa cctggcgatg gtggcgtcgt ccaggttgcg cttccagttg aaggacggcg
      241 agcagttgta ggccagcatc ttctcggggt acttcttgcg gattccttcg gcgaagcgct
      301 tggcgaaagt gaggtccggc gtgcccgttt cgcaccacac catgtcggcg tactccgcgt
      361 acgccaggcc gcgcgaaagc gcctggtcga atcccttgcg cgtcttgtag aagccctcgg
      421 aagtgcgttc gcctgtaatg aacggcttgt cgttctcgtc aatgtcgctc gtcacgatgt
      481 cggccgcctc ggcgtccgtg cgggcgagga gcacggtggg cacgcccatg atgtcggccg
      541 cgagccgcgc cgcgatgagc ttctggaccg cttcctgcgt cggcaccaac accttgccgc
      601 ccatgtggcc gcatttcttg accgaagcta gctggtcctc gtagtgcacg cccgccgcgc
      661 cggcctcgat catcgacttc atcagctcga acgcgttcag cacgccgccg aagcccgact
      721 ccgcgtcggc cacgatcggc gcgagccagt cgatgtcggg tgcggttgct tttcctccct
      781 gcgcttccat ggtctggatc tggtcggcgc gcaggagcgc gttgttgatc cttcgcacta
      841 ccgtggggac cgaggacact gcgtagagcg actggtcggg atacatgtgc agcgaatcgt
      901 tcgcatccgc cgccacctgc cagcccgaga gatagatcgc cggcacgccg gcgcgcacct
      961 gttgcaccgc ctggttgccc gtgagcgcgc cgagcgaatt gacgtatggc cgcgcattca
     1021 cgagcttcca gagcttctct gcgccgagcc tcgcgagcgt gtgctcgatc tgcacgctgc
     1081 cgcgcaggcg cacgacgtct tcggcgccat agccgcgctt tacatccttc cagcgcgggt
     1141 tttcggccca gtcttttttt gttgcttcga tcatcggtgc ggttcctcgc cagtggttgg
     1201 tgacccccca cgctacccct gccgcgtggc gcgcagcaac acgccagatg acctagttct
     1261 ctgttacaaa aacaaggctt tacatgggct tagagaggcc acgtttcacg atgcggcacc
     1321 gactttcagt tttgctgcaa cgcgccaaaa gccgcatcag cgctggacga gggtgcggtg
     1381 gcgctggacg ctcatcagga tgccgacgcc gatgaacagc gtaagaagcg cggtgccgcc
     1441 gtacgagagg aacggtaagg gcacgccgac cacgggcagg atgccggaca ccatgcccat
     1501 gttcacgaag gcgtaggtga agaacatcag ggtcaccgcg ccggcaagga ggcgcgcgaa
     1561 gagcgtcgcg gcgttggctg cgatgaccag gccgcggccg atgaggagcg cgtagatcac
     1621 gagcaggacg atgttgccga tcaggccgaa ctcctcggag tacaccgcga agatgaagtc
     1681 ggtgtgccgc tcggggatga actcgagatg ggcctgggtg ccattcagcc aacccttgcc
     1741 cgacacaccc cccgagccga ccgcgatcgt cgactggatg atgtggtagc cagcgccgag
     1801 aggatcctgc gtcggatcga ggagcgtgag cacacgcttt cgctggtaat cgtgtagcac
     1861 cccccacagc ggaaaaagtg cgaccaggaa tagggccgtc aagctgccga gcagcctcca
     1921 gcttacgccg gcgaagaaga tgacgtagaa gcccgccgcg ccgacgagcg cggcggtgcc
     1981 gaggtcgggc tggcgcacga tcaggccgac tggcatcgcc acaaggagcc cggcgaaggc
     2041 gaagtcgcgc aggcgcagcg tcgattcgcg gcggtggaaa taccacgcaa gcatgagcgg
     2101 caaggcaagc ttcatcatct ccgagggctg aaagcgcgtc accccgacgt gcagccagcg
     2161 ccgcgcaccg ttcacgacgt cgccgaacag cgcgaccgcg acgagcagcg cgagtcccag
     2221 caggtaggcc ggtacggcga aacgcatgag catttgcggg gaaatctgcg cggcgatcca
     2281 catcgcaacg agcgcgacgc agaggc
//
LOCUS       DASUNT010000263         2203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_35113, whole genome shotgun sequence.
ACCESSION   DASUNT010000263 DASUNT010000000
VERSION     DASUNT010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35113"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFX94_05195"
     CDS             complement(<1..295)
                     /locus_tag="VFX94_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5767613.1"
                     /translation="MIRNTFGFAACAAAALFWAGLAGAQQRVVLYTSNEATLNKLIAA
                     EFEKATGIAVDVISAGSGVVIKRLQSEKDRPQGDLVWGISRSLLQTNAQYFDSY"
     gene            complement(321..1418)
                     /locus_tag="VFX94_05200"
     CDS             complement(321..1418)
                     /locus_tag="VFX94_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX5767614.1"
                     /translation="MKGSSLRLRLPGPTMVPERIRLASAAPMQAHRGAEFKAMLAEVQ
                     DGLRAVYGTAQDVYLFAGSGTAMMEASLANVLAPGDAVLVAVAGQFSERFAAIATALG
                     AQVDTLDCEWGRGIEAQQLAERVGRKEYRALVVVHNESSTGVTEDLAAIGAALRERPT
                     LLLVDTVSGLAGTEMKMDAWGADIVVSASQKCLMCPPGLGFAAVSAKAWEVVKREGTV
                     PRFFYDFRRARAALEKGETAFTPPVSLVYGLREALRMIAEEGLVNVVARHRRLSSALR
                     EGCGKLGLEPYNRPAVASSTVVALKVPRGLEGTKIVAHVRSRYGTVIAGSRSPRLHNK
                     VIRIGTMGAVSEADIQTDLAHLEATLKDLIT"
     gene            complement(1405..1698)
                     /locus_tag="VFX94_05205"
     CDS             complement(1405..1698)
                     /locus_tag="VFX94_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX5767615.1"
                     /translation="MPKAYWVICHRSTKSAEQFAAFTKLAYPAIQAAGGRFLVRTSNV
                     AKTYEHGLNDRTVVIEFENLAKAIAAYDTPDYKEALRALADGAERDLRIVEGV"
     gene            complement(1691..>2203)
                     /locus_tag="VFX94_05210"
     CDS             complement(1691..>2203)
                     /locus_tag="VFX94_05210"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX5767616.1"
                     /translation="GVANCAPFSFFNVISEEPPLCILSFNWRSDREIKHTLKNIRRTG
                     EFVVNLADEGTANAMHLSSTELPEAESEFAKFGLTAVPAKLVKHPRIGEAAASLECRV
                     ERRIEFGPERELVVGEILLVHARDGIIDPVTKRISEELYRPVGRLFAERYCTTRERFN
                     LPGTLPKRHA"
BASE COUNT          353 a          747 c          730 g          373 t
ORIGIN      
        1 ggtaggaatc gaagtactgg gcgttggtct gcaagagcga gcggctgatg ccccagacga
       61 gatcgccctg cggccggtcc ttctcgctct gcaggcgctt gatgacgacg cccgagccgg
      121 ccgagatgac gtcgaccgcg atgcccgtcg ccttctcgaa ctccgccgcg atcagcttgt
      181 tgagcgtcgc ctcgttcgag gtatagagca cgacgcgctg ctgcgcgccg gccaggccgg
      241 cccaaaaaag cgcggcggcc gcgcaggccg cgaagccgaa ggtgttgcgg atcatcgtct
      301 cctcctcttt gtcgtgtgtt tcaggttatc agatccttta gcgtcgcctc gaggtgcgcg
      361 aggtcggtct ggatatcggc ttcgctgacc gcgcccatcg tgccgatgcg gatcaccttg
      421 ttgtgtagcc gcgggctgcg cgagccggcg atcacggtac cgtagcggct gcgcacgtgc
      481 gcgacgatct ttgtgccctc gagccctcgc ggcaccttga gcgccacgac cgtgctcgag
      541 gccacggccg ggcggttgta gggctcgagg ccgagcttgc cgcagccctc gcgcagagcc
      601 gacgagaggc gacggtggcg cgcaacgacg ttgacgaggc cctcctccgc gatcatgcgc
      661 agcgcctcgc gcaggccata aacgagcgac accggcggcg tgaatgccgt ctcgcccttt
      721 tccagtgcgg cccgggcgcg gcgaaaatca tagaagaagc gcggcaccgt gccctctcgc
      781 tttacgacct cccatgcctt cgcgctcacg gcggcgaagc ccagcccggg cgggcacatc
      841 aggcatttct gcgaggcgga cacgacgatg tccgcacccc acgcatccat tttcatctcc
      901 gtgccggcga gaccgctgac cgtatcgacg aggagcagcg tgggccgctc gcgcagcgcc
      961 gcgccgatag ccgcaaggtc ctcggtaacg ccggtggagc tttcgttgtg cacgacgacg
     1021 agcgcccgat actccttccg gccgacgcgc tcggcgagct gctgcgcctc gatgccccgg
     1081 ccccattcgc aatcgagcgt gtcgacttgc gccccgagcg ccgtggcgat ggccgcgaag
     1141 cgctcgctga actggccggc gacggcgacg agcaccgcgt cgcccggggc gagcacgttc
     1201 gcgaggctcg cctccatcat cgccgtgccc gaaccggcaa acaggtacac gtcctgcgcg
     1261 gtgccgtaga cggcgcgcag cccgtcctgc acttcggcga gcatcgcctt gaactcggcg
     1321 ccgcgatgcg cctgcatcgg cgcggcgctc gcgaggcgga tgcgctcggg caccatcgtc
     1381 gggcccggga ggcgcaggcg caggctagac cccttcaacg atgcgcaggt ctcgctccgc
     1441 gccatcggct agcgcgcgca gcgcttcctt atagtccgga gtgtcgtagg ccgcgatcgc
     1501 tttcgcgaga ttctcgaact cgatcaccac cgtgcggtcg ttgagcccgt gctcgtacgt
     1561 cttggccacg ttcgaggtgc ggaccagaaa ccggccgccg gcagcctgta tcgccggata
     1621 cgcaagcttg gtaaacgcgg cgaattgctc cgcgctcttg gtcgagcgat ggcagatcac
     1681 ccagtacgcc ttaggcatgt cgcttaggca acgtcccagg caagttgaag cgctcgcgag
     1741 ttgtgcaata gcgctccgca aacaagcggc cgacgggccg gtagagctcc tcggagatgc
     1801 gcttggtgac cgggtcgatg atgccgtcgc gcgcgtgtac cagcaggatc tcgcccacca
     1861 ccagctcgcg ctccgggccg aattcgatac gccgctcgac gcggcattca agcgaggcgg
     1921 cggcctcgcc gatgcggggg tgcttcacca gcttcgcagg cacggcggtg aggccgaact
     1981 ttgcgaactc gctctcggct tccgggagtt ccgtactgga aagatgcatc gcgttcgccg
     2041 tgccctcgtc agcgaggttg acgacgaact cgccggtgcg gcggatgttc ttcaaagtgt
     2101 gcttgatctc gcgatcggaa cgccagttga agctgaggat gcagagcggc ggctcctccg
     2161 agatcacgtt gaagaacgaa aacggcgcgc agttcgccac gcc
//
LOCUS       DASUNT010000264         3157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1263934, whole genome shotgun sequence.
ACCESSION   DASUNT010000264 DASUNT010000000
VERSION     DASUNT010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263934"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VFX94_05215"
     CDS             complement(<1..872)
                     /locus_tag="VFX94_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015422050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEX5767617.1"
                     /translation="MPYSVEGKLVVAISSRALFDFEEENRVFEKDGEPAYIALQYARL
                     DVPAREGVAFPLVKKLLAFNTPAQSRVEVVVLSKNDPVSGLRVFRSAKQAGLTIERGV
                     FTRGRSPYRYLDALKANLFLSANEADVMAALDAKVPAARVYPESTQAASRHADEVRIA
                     FDGDAVLFSDEAERVYQRDGLDAFTEHETARALQPLPPGPFKPLLEALHRLQTASGED
                     IAVRIRTALVTARSAPAHERAVRTLMEWNIAVDEAMFLGGLAKGEFLRSFEPDFYFDD
                     QLGHIDSARAFVAAG"
     gene            complement(925..2028)
                     /locus_tag="VFX94_05220"
     CDS             complement(925..2028)
                     /locus_tag="VFX94_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEX5767618.1"
                     /translation="MDVRAAVAWEAGKPLAIEKVQLAGPKAGEVLVEIKATGVCHTDE
                     FTRSGADPEGLFPAILGHEGAGVVVDVGPGVTSVRKGDHVIPLYTPECRQCKSCLSRK
                     TNLCTAIRATQGKGVMPDGTSRFSLNGKAVHHYMGCSTFANFTVLPEIAVAKIRDDAP
                     FDKVCYIGCGVTTGIGAVINTAKVEPGSNVVVFGLGGIGLNVVQGARMVGANMIVGVD
                     VNPAREALARKFGMTHFVNPKGKNVGAEIIELTGGGADYSFECIGNVDVMRQALECCH
                     RGWGVSVIIGVAGAGQEIRTRPFQLVTGRVWKGTAFGGARGRTDVPKIVDWYMEGRIN
                     IDDLITHVLPLERINDAFHLMHEGMSIRSVVVY"
     gene            complement(2049..2122)
                     /locus_tag="VFX94_05225"
     tRNA            complement(2049..2122)
                     /locus_tag="VFX94_05225"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2088..2090),aa:Gly,seq:ccc)
     gene            2143..2385
                     /locus_tag="VFX94_05230"
     CDS             2143..2385
                     /locus_tag="VFX94_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767619.1"
                     /translation="MGVIQRLVGVLLGLLFLAAVLVFASLALGVLIAVGLVIWGWLWW
                     RTRSIRRASGRGVVIEGEYRDVTRSQRIERREGPFR"
     gene            2695..2898
                     /gene="thiS"
                     /locus_tag="VFX94_05235"
     CDS             2695..2898
                     /gene="thiS"
                     /locus_tag="VFX94_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX5767620.1"
                     /translation="MIQVTVNGAAHRFDKPVHVSALLSSLSLEGRKVAVERNGEIVPK
                     SAHAATLLADGDRLEIVVAVGGG"
     gene            2900..>3157
                     /locus_tag="VFX94_05240"
     CDS             2900..>3157
                     /locus_tag="VFX94_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX5767621.1"
                     /translation="MKDSFIVAGKSYSSRLLIGTGKYRDFAQTRAAVEASGAEIVTVA
                     IRRTNIGQNAGEPSLLEALPPSKFTYLPNTAGCYSAEDAVRT"
BASE COUNT          537 a         1056 c         1016 g          548 t
ORIGIN      
        1 ccagcggcga cgaatgcacg cgccgagtcg atgtgcccaa gttggtcgtc gaagtagaag
       61 tcgggttcga atgacctcag gaactcgccc ttggcgaggc caccgaggaa catggcctcg
      121 tccaccgcga tgttccattc catgagcgtg cgcactgcgc gctcatgcgc cggcgcgctg
      181 cgggcggtga ccagggcagt tcttattcgg accgcaatgt cctcacccga agcagtctgc
      241 aaacggtgaa gtgcctccag gagcggcttg aacggcccgg gcggaagcgg ctggagcgcc
      301 cgcgccgtct cgtgctcggt gaacgcgtcc agcccgtcgc gctggtagac gcgctcggcc
      361 tcgtcggaaa agagcacggc gtcgccgtcg aacgcgatgc gcacctcgtc ggcgtggcgg
      421 ctcgccgcct gcgtggattc cgggtacacg cgcgcggccg gcaccttggc gtcgagcgcc
      481 gccatcacgt cggcctcgtt cgccgaaaga aagaggttcg ccttcaaagc gtcgaggtag
      541 cggtatgggc tgcgcccgcg cgtaaatacc ccgcgctcaa tcgtgagccc tgcctgcttg
      601 gccgagcgga acaccctgag gcccgacacc gggtcgttct tggacagcac caccacctcg
      661 acgcggctct gggccggcgt attgaacgcg agcagcttct tcacgagcgg aaacgccacc
      721 ccctcgcgcg ccggcacgtc gagccgggca tactgcagcg cgatgtacgc aggctcgccg
      781 tccttctcga atacgcggtt ctcctcctcg aagtcgaaca gcgcccgcga tgagatcgcg
      841 acgaccaact tcccttcgac ggagtacggc aatggacccc ctcgtcgtga ttatcccatc
      901 ctgagctgga cgctctctct gcggtcagta caccaccacg ctgcggatcg acatgccctc
      961 gtgcatcaga tggaacgcat cgttgatgcg ctcaaggggc agcacatgcg taatcagatc
     1021 gtcaatgttg attcggcctt ccatgtacca gtcgacgatc ttcggcacat cggtgcgccc
     1081 acgcgcgccg ccgaaagcgg tgcctttcca cacgcggccg gtcacgagct gaaacgggcg
     1141 ggtgcgaatc tcctggccgg cgccggcgac gccgatgatc acagacaccc cccagccgcg
     1201 atggcagcac tcgagcgcct ggcgcatcac atccacattg ccgatgcact cgaagctgta
     1261 atcggcgccg ccgccggtca gctcgatgat ttcggcgccc acgttcttgc ccttcggatt
     1321 gacgaagtgc gtcatcccga acttccgcgc cagtgcttcg cgtgcggggt tgacgtcgac
     1381 gccgacgatc atgttggcgc ccaccatgcg cgcgccctgc accacgttga gcccgatgcc
     1441 tcccaggccg aacaccacca cgttggatcc cggctccacc ttggcggtat tgatcacggc
     1501 gccgatgccg gtcgtcacgc cgcagccgat gtagcagacc ttgtcgaagg gggcgtcgtc
     1561 gcgaattttt gccaccgcga tctccggcag cacggtgaag ttggcgaagg tcgagcaccc
     1621 catgtagtga tgcacggctt tcccgttcaa cgagaagcgg ctcgtgccgt cgggcatgac
     1681 ccccttgccc tgcgtagcgc gaatcgccgt gcaaaggttc gtcttgcgcg aaaggcagga
     1741 cttgcactgg cggcactcgg gcgtgtagag cgggatgaca tggtcgccct tccgcacact
     1801 ggtgacgccc gggcccacgt cgaccactac cccggcgcct tcgtggccaa ggatggcggg
     1861 gaaaagcccc tcgggatccg cgcccgagcg ggtgaactcg tcggtgtggc acacgccggt
     1921 cgccttgatc tcgaccagca cttcgccggc cttgggcccg gcgagctgca ccttctcgat
     1981 cgcgagcggc ttgcccgctt cccacgccac ggcagcgcgt acgtccatta agggctttcc
     2041 cgaaaatttg gagcgggtga tgggaatcga acccacgtat tcagcttggg aagctgatgt
     2101 tctgccattg aactacaccc gcattgagcg aatcatattc caatgggggt catacagcgt
     2161 ctcgtagggg ttttgctcgg cctgctgttt ctggcagcgg tcctggtgtt cgcttcgctc
     2221 gcgctcggcg tgctgattgc ggtgggcctc gtcatctggg gctggttgtg gtggcgcacc
     2281 cgttccatac gccgcgcttc cgggcgcggc gtggtaatcg agggcgagta ccgcgacgtt
     2341 acgcgctcgc agcgcatcga gcgccgcgaa ggcccgtttc gctgatcaga acacgtagtc
     2401 gagcccgaga agcagcgtcg agtcggtgct cacgcgcccc ggcgccgggc gccgcggacc
     2461 ccgcactgaa gtgcgggacg ttgcccgcgc tgtcgacgag agacacggat cggacgtcgg
     2521 ccaggcggat cgagatttgt cccgcgtatt cggtctccag gacgagatgc gcgccgctcc
     2581 tcgccttgag ctcgccgctg atccgatcgc cattgacgag ggtccctcgt cggcaagcgc
     2641 cgggagaccg agcaggcaaa cgatgagcgc cgttccgcgc ggtatcattg gccgatgata
     2701 caagtcacgg taaacggcgc cgcgcaccgc tttgacaagc cggtccacgt ttcggcgctg
     2761 ctctcgtcgc tcagcctcga gggcaggaaa gtcgcggtgg agcggaacgg cgagatcgtg
     2821 ccgaagagcg cgcacgcagc cacgctgctc gccgacggcg accgcctcga gatcgtggtc
     2881 gcggtcggag gaggataaag tgaaggactc gttcatcgtt gcgggcaaga gctattcgtc
     2941 gcggctgctg atcggcacgg gaaaataccg cgacttcgca cagacgcgcg cggcggtgga
     3001 ggcttccggt gccgagatcg tcacggtggc gatccgccgc accaacatcg gccagaacgc
     3061 cggcgagccg agcctgctcg aggcgctgcc gccctcgaaa ttcacctacc tgcccaacac
     3121 cgcgggctgc tacagcgccg aggacgcggt gcgcacg
//
LOCUS       DASUNT010000265         5145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_211318, whole genome shotgun sequence.
ACCESSION   DASUNT010000265 DASUNT010000000
VERSION     DASUNT010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211318"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VFX94_05245"
     CDS             <1..360
                     /locus_tag="VFX94_05245"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family substrate-binding domain-containing
                     protein"
                     /protein_id="HEX5767622.1"
                     /translation="GRVALRELREEAFVMVPRDIAPGLYDIVAGLAARAGFSPRVAQE
                     AIQMQTVVSLVSSGLGVAVVPGSIANLGRRGVVYRQLADPHPQLELWLAWRRGSLSAA
                     GRELVELAKYRLRKAPA"
     gene            357..1127
                     /gene="lgt"
                     /locus_tag="VFX94_05250"
     CDS             357..1127
                     /gene="lgt"
                     /locus_tag="VFX94_05250"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX5767623.1"
                     /translation="MIAHPNIDPIAFSIGPLAVRWYGLMYLAGFAIGWWLGARRIARG
                     QAPITRAQFDDLLFLVVLGVILGGRLGYVLFYKPGYYASHPLEIFAIWQGGMSFHGGL
                     LGVMAAMVFAARRAGVDYVRLMDFIAPLVPPGIAAGRFGNFINAELPGRVTDLPWGMV
                     FPGTEGAPRHPSQLYQFALEGLALFALLWWFSSRPRPRGQASAMFLLGYGVLRFIAEF
                     AREPDAFIGYLALGFTMGQWLCLGMIVAGAALFAWSRR"
     gene            1145..2044
                     /locus_tag="VFX94_05255"
     CDS             1145..2044
                     /locus_tag="VFX94_05255"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX5767624.1"
                     /translation="MLPVFGLVGCGYLAGRLRIVTQASSAALNQFVYAFALPAMLFVA
                     VYRGSLEEILSGAFMLAVIAATISTAAVGFLISSASGAPPAENTMRALNASFANTGYL
                     GIPLVTVAYGEAAALPAALATVATNFVSFALAIVCLELFVNPHPGALRRALRGVVMSP
                     LIWPIGLALLLVSIQIRVPLPVERLATLLAAAAGPCALFAIGLFVSQLSIRAGAVASW
                     QTTVLKLVLHPLLMALLVFIVLDIDRLWAKTAVVGAALPLGATAFVLAQRYKLLEAET
                     STGAVVSTAASLLTVSLVMALLG"
     gene            complement(2041..4827)
                     /locus_tag="VFX94_05260"
     CDS             complement(2041..4827)
                     /locus_tag="VFX94_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5767625.1"
                     /translation="MNAPLTPVSFAPLERSSFAERLRRELDAEILFDAASRGRYSTDA
                     SIYQVEPIGVVVPRSEEAARGAIAIAVEHGVPILPRGAGSSQCGQAVGAALVIDHTKY
                     LNKILEIGPSHVVVQPGVVLDQLNAALRKHGVWFPVDVSTSAQATLGGMAGNNSCGSR
                     SIEYGNMVHNVLAIDAVTASGHRWRFGPLKEASGAYAEFVNKLERLYERERAEIEARF
                     PKVLRKVAGYNLDHLGPPHANAAHLLVGSEGTLAYSERLHLKLAPLPGKRALGVCHFR
                     RFYTAMELTQHIVKLAPTAVELVDRTMIGLARENPAFRSVVDSFIRGDPDAILLVEFA
                     HERVGRLKALVQLMADLGHPGSVVEITEEKLQKQVWEVRKAGLNIMMSMKGDGKPVSF
                     IEDCAVPLEHLAEYTERLTRVFEKHGTRGTWYAHASVGTLHVRPVLDMRRDGARKMRA
                     IAEEACAMVKQYKGAYSGEHGDGLVRSEWIAPFFGPRLTAALGEIKSWLDPRGLMNPG
                     KIVNPSRMDDRTLFRFRPGYSTTVPPTVLDWSEWGGYDKAVEMCNNNGHCRKFDAGTM
                     CPSYRATMDEQHLTRGRANTLRLALSNQLNFDEAREALDLCVSCKGCKRECPTGVDMA
                     RMKIEFLAHYKAKHGWSARDKAIAYVPRYAASAARLAPVSNVVASLAKRSLGFSEKRD
                     LPRWRTDFFADEGRRDGDVVLLVDTFNRYFEPENARAALKVLEAAGYGVYAPRGLCCG
                     RTFLSVGLVDEAKREARKMIDALAPYVGRGVPIVGLEPSCLFSLRDEFPVLAGDELAK
                     GSFLLEEFLAGRPGKLRFREMERQVLLHGHCHQKAFDAMPAVEKVLGMVPGLRISTVQ
                     TSCCGMAGSFGYEAEHYEVSMKMAELSLLPAIRSAPAQTLLVADGTSCRHQIADGSGR
                     AAVHAARVLEQALL"
     gene            4852..>5145
                     /locus_tag="VFX94_05265"
     CDS             4852..>5145
                     /locus_tag="VFX94_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--glyoxylate aminotransferase"
                     /protein_id="HEX5767626.1"
                     /translation="MTRRAGRHFLQIPGPTNVPDRVLRAIDFPTIDHRGPEFAALGKE
                     VLAGMRHVFKTTQGEVVIYPASGTGAWEAALVNTLSPGDRVLMVETGHFATLWQ"
BASE COUNT          770 a         1713 c         1700 g          962 t
ORIGIN      
        1 gggcgcgtgg cgctgcgcga gctgagggaa gaggcgttcg tcatggtgcc gcgggatatc
       61 gcgccgggcc tctacgatat agtcgccggg ctcgcggcgc gcgccggctt ctcgccgcgg
      121 gtggcgcagg aggcgattca aatgcagacg gtcgtgagcc tggtgtccag cgggctcggc
      181 gtcgccgtgg ttcccggctc gatcgcgaac ttgggccggc gcggcgtcgt gtaccggcag
      241 ctcgccgacc cgcacccgca actcgagctg tggctcgcct ggcggcgcgg cagccttagc
      301 gcggcgggac gcgagctggt ggagctggcg aagtacaggt tgcggaaagc acccgcgtga
      361 tcgcgcaccc gaacatcgac ccgatcgcct tctcgatcgg gccgctcgcg gtgcgctggt
      421 acggcctcat gtacctcgcc ggcttcgcca tcggctggtg gctcggggca aggcgcatcg
      481 cccgcgggca ggcgccgatc acacgggcgc agttcgacga ccttcttttc ctcgtggtgc
      541 tcggggtgat cctcggcggg cgcctgggtt acgtgctgtt ctacaagccg ggctactacg
      601 cttcgcatcc gctcgagatc ttcgccatct ggcagggcgg catgtcattc catggcgggc
      661 tgcttggcgt gatggcggcc atggtgtttg ccgcgcgccg cgccggggtc gattacgtgc
      721 gcctgatgga cttcatcgcg ccgctggttc cgccgggaat cgcggccggc aggttcggca
      781 acttcatcaa cgcggagctg cctggccggg tgactgatct gccgtggggc atggtgttcc
      841 cgggcacgga gggcgccccg cggcatcctt cgcagctcta ccagttcgcg ctcgaaggac
      901 tcgcgctgtt tgctttactg tggtggttct cgtccaggcc gcggccgcgc gggcaggcat
      961 cggcgatgtt tttgctcggc tacggagtgc tgcgcttcat tgccgagttc gcccgcgagc
     1021 ctgatgcgtt catcggctat ctggcgcttg gcttcaccat ggggcagtgg ctctgcctcg
     1081 ggatgatcgt cgccggcgcc gcgcttttcg catggagtcg gcgctagacc cggttctcgg
     1141 agctgtgctt cccgtattcg ggctcgtcgg ctgcggatac ctggccgggc gcttgcgcat
     1201 cgtcacgcag gcatcgagcg cagccctgaa ccagtttgtc tacgcgttcg ctctcccggc
     1261 aatgcttttc gtggcggttt accgcggctc gctcgaggag atcctgagcg gcgctttcat
     1321 gctcgccgtt atcgcggcca cgatcagcac cgcagccgtc gggttcctca tttcaagcgc
     1381 ttcgggcgct ccgccggcgg agaacacgat gcgcgcgctg aacgcatcgt tcgcgaacac
     1441 gggctacctc ggaataccgc tcgtcaccgt ggcctacggc gaggccgccg ccctgccggc
     1501 ggcgcttgct accgtggcga ccaatttcgt ctcgttcgct ctggccatcg tgtgcctgga
     1561 gcttttcgtg aacccgcatc ccggtgcgct gcgccgcgcg ctgcgcggcg tcgtcatgag
     1621 tccgctcata tggccgattg ggttggccct cctgcttgtt tcaatccaaa tacgagttcc
     1681 gttgccggtg gagcgcctcg cgacgctgct cgcggcggcg gcgggtccgt gtgcgctgtt
     1741 cgcgatcggg ctgttcgtgt cgcagctgtc gatccgcgcc ggcgcggtcg cgagctggca
     1801 aaccacggtg ctgaagctcg ttctgcaccc gctgctgatg gcgctgctcg tctttatcgt
     1861 tctcgacatc gaccgcttat gggccaagac cgccgtggtg ggtgcggcgc ttccgcttgg
     1921 cgccactgcc tttgtcctcg cccagcgcta caagctgctg gaagccgaga cttcgaccgg
     1981 cgccgtcgtt tccaccgcgg cttcgctcct cacggtctcg cttgtgatgg cgcttctcgg
     2041 ctaaagcagc gcctgctcca gcacgcgcgc tgcgtggacc gcggcgcggc cgctgccatc
     2101 ggcgatctgg tgacggcagc tcgtgccgtc ggcgacgagg agcgtctgcg ctggagcact
     2161 ccggatggcg ggcagcagcg agagctcggc catcttcatc gagacctcgt agtgctccgc
     2221 ctcgtagccg aagcttcccg ccatgccgca acagctcgtc tgcacggtgg agatcctcag
     2281 gccgggcacc atcccgagca ccttctcgac cgcgggcatg gcgtcgaacg ccttctggtg
     2341 gcagtgtcca tgcagcagaa cttgccgctc catttctctg aacctcagct tgccgggccg
     2401 gcccgccagg aactcttcca gcaggaaaga gcccttcgcg agctcgtcgc ccgccaaaac
     2461 gggaaactcg tcgcgcaacg aaaaaaggca cgagggctcg aggccgacga tcggcacgcc
     2521 tcttcccaca tagggcgcca gcgcgtcgat catcttgcgc gcttcgcgct ttgcttcgtc
     2581 gacgagcccc accgaaagga atgtgcggcc acaacacaag cctcgcgggg catagacacc
     2641 atagcccgct gcctccagta ccttgagcgc ggcgcgggcg ttttccggct cgaagtaccg
     2701 gttgaacgtg tccaccaaca gcaccacatc gccgtcgcgc cttccttcgt cggcaaagaa
     2761 gtccgtgcgc cagcgtggca ggtcgcgctt ctcggagaag ccaagcgagc gcttcgccag
     2821 cgaagcaacg acattcgaaa ccggcgcgag ccgcgcggcg gaggccgcat agcgcggcac
     2881 gtaggcgatc gccttgtcgc gggcgctcca gccgtgcttt gccttgtagt gcgccaggaa
     2941 ctcgatcttc atgcgcgcca tatcgacgcc ggtggggcac tcgcgcttgc agcctttgca
     3001 cgagacgcac agatcgagcg cttccctcgc ttcatcgaag ttcaactgat tggaaagcgc
     3061 aagccgcagc gtatttgcgc ggcctcgcgt caaatgctgc tcgtccatcg tcgcgcggta
     3121 cgacgggcac atcgtgccgg cgtcgaactt gcggcagtgg ccgttgttgt tgcacatctc
     3181 gactgccttg tcgtagccgc cccattcgct ccagtcgagc accgtcggcg gcacggtggt
     3241 gctgtagccc ggcctgaagc ggaacagcgt gcgatcgtcc atgcgcgacg ggttcacgat
     3301 cttgccgggg ttcatcagcc cgcgcgggtc gagccaggac ttgatctcgc ccagcgcggc
     3361 agtcagccgc gggccgaaga acggcgcgat ccattcggag cgcaccaggc cgtcgccgtg
     3421 ctcgccggaa taggcgcctt tgtactgctt caccatggcg cacgcttcct ccgcgatggc
     3481 gcgcattttt cgtgcaccgt cgcgccgcat gtcgagcacc ggccgcacgt gaagcgtgcc
     3541 gaccgaggcg tgcgcgtacc aggtgccgcg cgtgccgtgc ttctcgaaca cccgcgtaag
     3601 ccgctcggtg tactccgcga gatgctcgag cggcaccgcg cagtcctcga taaaggacac
     3661 cggctttccg tcgcccttca tcgacatcat gatgttgagg ccggccttgc gcacttccca
     3721 cacctgcttc tggagcttct cctcggtgat ctcgaccacg ctcccgggat ggcccaggtc
     3781 ggccatgagc tgcaccagag ccttcagccg gccgacgcgc tcgtgggcaa actcgacgag
     3841 caggatggca tcggggtctc cgcggatgaa cgaatccacg acactgcgga atgccggatt
     3901 ctcacgcgcg agcccgatca tcgtgcggtc gacgagctcg acggccgtgg gcgcgagctt
     3961 cacgatgtgc tgcgtgagct ccatcgcggt gtagaacctg cggaagtggc acaccccgag
     4021 cgcgcgcttc ccgggtagcg gcgcgagctt taggtgcaag cgctcggagt aggcgagcgt
     4081 gccctcggag ccgacgagaa gatgcgccgc attggcgtgt ggcgggccga ggtgatccag
     4141 gttatagccg gcgacctttc tgaggacttt gggaaagcgg gcttctatct cggcgcgttc
     4201 gcgctcgtac agccgttcga gcttgtttac aaactccgca taagcgccgg aggcctcctt
     4261 caaggggccg aagcgccagc gatggcccga agccgtgaca gcgtcgatcg ccagtacgtt
     4321 atgcaccatg ttgccgtact cgatcgagcg cgagccgcag gaattattgc cggccatgcc
     4381 gccgagggtc gcctgcgcgc tcgtcgagac gtcgaccggg aaccacaccc cgtgcttgcg
     4441 cagcgcggca ttcagttggt cgagtaccac gccgggctgc acgacaacat gcgatgggcc
     4501 gatctcgagg atcttgttga gatacttcgt atgatcgatg acgagcgccg cgccgaccgc
     4561 ctggccgcac tgcgagctgc ccgcgccgcg cggcaggatc gggacgccgt gctccaccgc
     4621 gatcgcaatt gcgccacgcg cggcttcttc gctgcgcggc acaacgacgc cgatcggttc
     4681 cacctggtag atcgaggcat cggtggagta gcggccgcgg cttgcggcgt cgaacaggat
     4741 ttcagcgtcg agctcgcggc gcagccgctc ggcgaaagag gaacgctcaa gaggcgcaaa
     4801 actgaccggg gtcaaggggg cattcatggg agccgtggtt agaataaccc aatgacccga
     4861 cgcgccggcc gccatttcct gcagattccg ggaccgacca acgtgcccga tcgcgtgttg
     4921 cgcgcgatcg atttcccgac catcgaccat cgcggcccgg agttcgccgc gctcggcaag
     4981 gaagtgctgg cgggaatgag gcacgtcttc aagaccacgc agggcgaggt cgtgatttat
     5041 cccgcctcgg gaaccggcgc atgggaggcg gcgctcgtga acacgctttc tcccggcgat
     5101 cgcgtgctca tggtcgagac cgggcatttc gccacgctgt ggcag
//
LOCUS       DASUNT010000266         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1264315, whole genome shotgun sequence.
ACCESSION   DASUNT010000266 DASUNT010000000
VERSION     DASUNT010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264315"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..877)
                     /locus_tag="VFX94_05270"
     CDS             complement(68..877)
                     /locus_tag="VFX94_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5767627.1"
                     /translation="METIFVGPAPRLAVSIAGKGELLFFLHGIGGNRRQWDAQLAFFS
                     ARYRAAAWDARGYGDSEDYEGPLQFSTFSADLVRVLDHLGADKAHVVGLSMGGRIARD
                     FALRHPKRVRSLTLANTSPGFDALTADQVKAFLDARRAPLLAGKSPAELAPDLARKLA
                     GPRASGAAIEALVASISRLHKESYLKTLEASVTQDRAAPVERLAVPTLVVTSEHDSLY
                     PPQLARAMAARIGGAELVEIPGAGHVSSLEQPERFNAALAAFLVKHQRQED"
     gene            complement(879..1838)
                     /locus_tag="VFX94_05275"
     CDS             complement(879..1838)
                     /locus_tag="VFX94_05275"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5767628.1"
                     /translation="MKLVLAFLLLASSTAALGQVWPSKPVRIVVAYPPGGGIDVMARQ
                     LADKLQPVWGQPVIVENKPGANTIVATDSVAKSAPDGHTILMTTDATFSINPHLYSKL
                     PYDARRDFFAATMLVLLHQFLVAHRSLPANSLAELVALAKAKPGTINYASYGSGSQPH
                     LAGEMLKHKAGIDLVHVPYKGISLAVPAVVAGEVQLTFAGIATSTPQLKAGRIKALAI
                     GGPSRSPLFPEVPTFTELGYPEVETHAWFGLFVPAGSPREAVSRIHRDTRKILDEPEF
                     RQTQLIDKGYEVVGSSPEEFAAYIAKDSESRARAVKISGAKAE"
     gene            1877..>2063
                     /locus_tag="VFX94_05280"
     CDS             1877..>2063
                     /locus_tag="VFX94_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5767629.1"
                     /translation="MKIVIVGGGIGGLSLARELGLRGMKPTVLERAPQLNPVGAGIIM
                     NPNAMRVLEKNGLAEAVR"
BASE COUNT          330 a          671 c          724 g          338 t
ORIGIN      
        1 cgccgcccat ctcgacatct cgcgacaggc ttatctgcaa acgcatcgcc tgcgctttcc
       61 gggctgatca atcttcttgt cgttggtgct tcacgagaaa ggccgcgagc gcggcgttga
      121 agcgctcggg ctgctcgagg ctcgagacat gccccgcgcc cgggatctcc acgagctcgg
      181 cgccgccgat gcgcgcggcc atggcgcgcg cgagctgcgg cgggtagagc gagtcgtgct
      241 cgctcgtcac gacgagcgtc ggtacggcga ggcgctcgac cggcgcggcg cggtcctgcg
      301 tcaccgaggc ttcgagcgtt ttcaggtacg actccttgtg caggcgcgaa atgctggcga
      361 cgagcgcctc gattgcggcg cccgaggccc gcggcccggc gagcttgcgg gcgagatcgg
      421 gcgccagctc ggccggcgac ttgccggcga gaagcggtgc gcgccgcgcg tccaggaacg
      481 ccttgacctg gtcggcagtc agcgcgtcga accccgggct ggtgttcgcc agcgtgagcg
      541 agcgcacgcg cttcggatgg cgcagcgcga agtcgcgcgc gatgcgccct cccatggaga
      601 gacccacgac gtgcgcctta tcggcgccga ggtgatcgag tacccgcacg agatcggccg
      661 agaaggtgga gaactggagc gggccttcgt aatcttctga atcgccgtag ccgcgggcgt
      721 cccaggcggc cgcgcggtag cgcgcggaaa aaaaggcgag ctgcgcatcc cactgacggc
      781 gattgccgcc gatgccgtga aggaagaaaa gcagctcccc ctttccggcg atcgagaccg
      841 ccaggcgcgg cgccggaccg acgaagatcg tttccaggct actcggcctt cgcgccggag
      901 atcttcaccg cacgcgcacg gctctcgctg tccttcgcga tgtaggccgc gaactcttcc
      961 gggctgctcc cgacgacctc gtagcccttg tcgatgagct gcgtctggcg aaactcgggc
     1021 tcgtccagga tcttgcgcgt gtcgcgatgg attcgggaca ccgcctcgcg cggcgagccc
     1081 gccggaacga agagcccgaa ccacgcgtgt gtttccacct ccgggtagcc cagctcggtg
     1141 aatgtcggca cttcgggaaa aagcggcgag cggctcgggc cgccgatggc gagtgccttg
     1201 atgcggcccg ccttgagctg cggtgtggac gtcgcgatgc cggcgaaggt gagctgcacc
     1261 tcgccggcga cgaccgccgg caccgcgagc gatattccct tgtacggcac gtgtaccagg
     1321 tcgattccgg ccttgtgctt gagcatctcg ccggcgaggt gcggctggct gccgctgccg
     1381 taggaagcgt agttgatggt tccgggcttc gccttcgcca gcgcgaccag ttcggcaagg
     1441 ctgttggccg gcagcgaccg gtgcgcgacc aggaactggt gcaagagcac cagcatggtc
     1501 gccgcaaaga agtcgcgccg cgcgtcatag ggcagcttgc tgtacaggtg cgggttgatg
     1561 gagaacgttg catccgtcgt catgaggatg gtgtggccat cgggggcgct cttcgcgacg
     1621 gaatccgtgg ccacgatggt gtttgcgccg ggtttgttct cgacgatgac cggctgtccc
     1681 cacaccggct gcagcttgtc ggcgagctgg cgcgccatca cgtcgatccc gccgccgggc
     1741 ggataggcga ccacgatgcg caccggcttc gacggccaca cctggccgag cgctgccgtg
     1801 gaagacgcga gcaacagaaa agccaacacg agcttcatgc gattcctcct cgtcgtggcg
     1861 attatgatgt aaagccgtga agatcgtgat cgttggcggc ggtatcggcg gactctcgct
     1921 cgcgcgcgag ctcggcctgc gagggatgaa gcccacggtc ctggaacggg caccacagct
     1981 caaccccgtg ggcgccggga tcatcatgaa cccgaacgcc atgcgcgtgc tcgagaagaa
     2041 cggcctcgcg gaggcggtgc ggc
//
LOCUS       DASUNT010000267         2859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_825683, whole genome shotgun sequence.
ACCESSION   DASUNT010000267 DASUNT010000000
VERSION     DASUNT010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825683"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VFX94_05285"
     CDS             <1..667
                     /locus_tag="VFX94_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HEX5767630.1"
                     /translation="RHVVLLDGGNASQADYAGIAAERAALKAYLQDLSGVSEAQFNAW
                     SEPEQIAFLINAYNAYTVEKILTRYPDIRSIWDFGKFFGNPFRDEFFSLLGQKTSLDG
                     IEHGILRKRYREPRIHYAVSCASIGCPMLREEAYVAARLQEQLEEQARRFLSDRSRNR
                     WQGARLEVSKIFDWYKEDFEPREQYFARYAQLLGMPPGASPRLVFLDYDWALNDSRSS
                     SPR"
     gene            628..1305
                     /locus_tag="VFX94_05290"
     CDS             628..1305
                     /locus_tag="VFX94_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX5767631.1"
                     /translation="MGAQRLSIVVPALNEARGIRAALEALAPLRKRGHEVIVVDGGST
                     DGTTELARGLCERMLGAPRGRARQMNAGAAAASGDALVFLHADTRLPDAADDLILGSL
                     RSFLWGRFDVEIVGRHRLLKLVACAMNVRSRLTGIATGDQAIFVRRDAFAGFPEIALM
                     EDVAFCKAMKRFGPPACRRERVVTSGRRWESRGVLRTIVLMWRLRLMYFLGACPERLA
                     RIYYHYR"
     gene            1302..1883
                     /locus_tag="VFX94_05295"
     CDS             1302..1883
                     /locus_tag="VFX94_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX5767632.1"
                     /translation="MIIVFAKAPVPGEAKTRLIPRLGAWRAARLHVRLTLHALRTARA
                     AHCGPVELHLTRRHSLFKGARLQRGRDLGERMQHAFRRSRQAILIGTDCPVLGARDLQ
                     RAARWLRGGYDAVVAPAEDGGYALIGLKKPLLHLFRGIDWGSERVYAQTVKKLAGYRW
                     RALPSVWDLDRPQDLERLRSLRLPLASRPAARR"
     gene            1993..2469
                     /locus_tag="VFX94_05300"
     CDS             1993..2469
                     /locus_tag="VFX94_05300"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX5767633.1"
                     /translation="MQVRIRAPKDFWAGLMFIAFGLFFMVWALTHYQMGSAVRMGPAY
                     FPAVLGGLLAFLGALVLIESLTMQGPRLPKFGLRPLVLISVACVIYGYAMKPLGLIIA
                     TALLVFISAFGGHEFKWKEVAILYVILIVFSLLVFVKGLTLPFPVCPAFMDTCPIR"
     gene            2479..>2859
                     /locus_tag="VFX94_05305"
     CDS             2479..>2859
                     /locus_tag="VFX94_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5767634.1"
                     /translation="MDVLNNLILGFGVAISPLHLLYALIGVMLGTLIGVLPGIGPVAT
                     IAMLLPITFNLSPIAALIMLAGIYYGAQYGGSTTSILVNLPGEASSVVTCLDGHQMAR
                     QGRAGAALAVAAIASFFGGTVATLV"
BASE COUNT          409 a          974 c          963 g          513 t
ORIGIN      
        1 cagacatgtc gtgctgctcg acggcggcaa cgcttcgcag gccgactacg cgggaattgc
       61 cgccgagcgt gccgcgctca aggcatacct gcaggacttg tcgggcgtga gcgaggcgca
      121 gttcaacgcc tggagcgagc ccgagcagat cgccttcctc atcaatgcct acaacgccta
      181 caccgtcgag aagatactga cgcgctatcc ggacattcgt tccatctggg acttcggcaa
      241 gtttttcggc aaccctttcc gcgacgagtt tttttcgctg ctcgggcaga agacgagcct
      301 cgacggcatc gagcacggca tcctgcgcaa gcgctaccgc gagccgcgca tccactacgc
      361 ggtgagctgc gcctccatcg gctgtccgat gctgcgcgag gaagcctacg tcgccgcgcg
      421 cctgcaggag cagctcgaag agcaggcgcg gcgctttctc tccgaccgct cgcgcaaccg
      481 ctggcagggc gcacggctgg aggtctcgaa gatcttcgac tggtacaagg aggatttcga
      541 gccgcgcgag caatacttcg cgcgctacgc gcagctgctc ggcatgccgc cgggcgcgag
      601 cccgcggctc gtgttcctcg actacgattg ggcgctcaac gactctcgat cgtcgtcccc
      661 gcgctgaacg aggcgcgcgg catccgcgcc gcgctcgagg cgctggcgcc gctgcgcaag
      721 cgcggccacg aggtgatcgt ggtggacggc ggcagcacgg acgggaccac ggagctcgcg
      781 cgaggactgt gcgagcgcat gctcggcgct ccgcgcgggc gtgcccgcca gatgaatgcc
      841 ggagccgcgg cggcgagcgg cgatgcgctc gtttttctgc acgccgatac ccgtctgccg
      901 gacgcggcgg acgatctcat cctgggttcg ctgcgttcct tcctctgggg aaggttcgac
      961 gttgagatcg tcgggcgcca tcggctgctg aagctcgtcg cctgcgccat gaacgtgcgc
     1021 tcgcgcctca ctggcatcgc gaccggcgac caggcgattt tcgtgcgccg cgacgcgttt
     1081 gccggcttcc cggaaatcgc gctcatggag gacgtggcgt tctgcaaggc gatgaagcgc
     1141 ttcgggccgc ctgcatgccg gcgggaacgg gtagtgacct ccggccggcg ctgggagtcg
     1201 cgcggcgtgc tgcgcacgat cgtcctcatg tggcggcttc gcctcatgta cttcctgggt
     1261 gcctgccccg agcggctcgc gcgcatctac taccactacc ggtgatcatc gtcttcgcca
     1321 aggcgccagt gcccggcgag gcgaagacgc gcctgatacc gcggcttggc gcctggcgcg
     1381 ccgcccgcct gcatgtgcgc ctcacgctcc atgccttgcg caccgcgcgc gctgcgcact
     1441 gcgggccggt cgagcttcat ctcacgcgcc gtcactcgct tttcaaaggg gcgcggctgc
     1501 agcgcgggcg cgatctcggc gagcgcatgc agcacgcatt ccgcaggtcc cgacaggcga
     1561 ttctcatcgg caccgactgc ccggtcctcg gcgcgcgcga tctgcagcgc gcggcgcgct
     1621 ggctgcgcgg cggctacgac gccgtggtgg ccccggccga ggacggcggc tacgcgttga
     1681 tcgggctgaa aaagcctctg ctgcatcttt tccggggcat cgactgggga agcgaacgcg
     1741 tgtacgcgca gacggtgaag aaactcgccg gctatcgctg gcgcgcgctg ccgagcgtct
     1801 gggacctcga ccggccgcag gacctcgagc gcctcagatc gctccgcctc cccttggcgt
     1861 ctcggccagc cgctcggcga tgagcgagct gccggagaaa atcgttgccg ccgatgcaga
     1921 gcagcaaagc cgcggcgaat cgcatcgggg gcgcgtgcta cactttgccg cgccagcggc
     1981 ggaggagagg caatgcaagt caggatcagg gctccgaagg atttctgggc cgggctgatg
     2041 ttcatcgcgt tcggcctgtt cttcatggtc tgggcgctca cccactacca gatgggcagc
     2101 gccgtgcgca tggggccggc gtacttcccg gccgtgctcg gcgggctgct cgccttcctc
     2161 ggcgcgctgg tgctgatcga gtcgttgacc atgcaaggtc cgcggctgcc gaaattcggg
     2221 ttgcggccgc tggtcctcat ctcggtggcg tgcgtgattt acggctacgc gatgaagccg
     2281 cttggcctca tcatcgccac ggcgcttctg gtgttcatca gcgcgttcgg aggccacgag
     2341 ttcaagtgga aagaagtggc catcctgtac gtgatcctca tcgtgttctc gctgctcgtg
     2401 ttcgtgaagg ggctgacgct gccgttcccg gtctgcccgg cgttcatgga cacctgcccg
     2461 atccgctagg gagccgcgat ggatgtcctc aacaacctga tcctcggctt cggcgtcgcg
     2521 atctcgccgc tgcacctgct ctacgcgctg atcggcgtca tgctcggcac gctcatcggc
     2581 gtgctgcccg gcatcggccc ggtggccacg atcgcgatgc tgctgccgat tacgttcaac
     2641 ttgagcccga tcgccgcgct gatcatgctt gccggcatct attacggcgc gcagtacggc
     2701 ggctccacca cctcgatcct cgtgaacctc cccggcgagg cgtcgtcggt cgtgacctgc
     2761 ctcgacggcc accagatggc gcggcaggga agggcgggag cggcgctcgc cgtcgcggcg
     2821 atcgcgtcct tcttcggcgg gaccgtggcc accctggtc
//
LOCUS       DASUNT010000268         5833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_650199, whole genome shotgun sequence.
ACCESSION   DASUNT010000268 DASUNT010000000
VERSION     DASUNT010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650199"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1104
                     /locus_tag="VFX94_05310"
     CDS             196..1104
                     /locus_tag="VFX94_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5767635.1"
                     /translation="MDRLGAIQVFAQVVESGSFAKAAERLGLSTSATSRHVADLEAHL
                     QTRLLNRTTRRVSLTESGRAFYERAVQLLADLEEAEQEAARAAVVPRGTIRLTTSVNF
                     GVRHLAPAIAEFLGRHAEVRFDVSLSDRVVDLIEEGFDLAVRLGTTGSENVVARKLGE
                     TRLVPCASPQYLSAHPAPKAPEDLAAHNCFTYEYITPRNVWRFRDAAGGERAVRIAGT
                     LHSNNGDLLAEIAARGCGIVFEPAFIVGPDVRAGRLVPLLQDFMPPPVPIYAVYPSRK
                     HLSAKVRAFVDFLVTRFEDAPDWSAA"
     gene            1506..2186
                     /locus_tag="VFX94_05315"
                     /pseudo
     CDS             1506..2186
                     /locus_tag="VFX94_05315"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
     gene            complement(2254..2805)
                     /locus_tag="VFX94_05320"
     CDS             complement(2254..2805)
                     /locus_tag="VFX94_05320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX5767636.1"
                     /translation="MTPRAIAIRLAEPRDAQAIATMSRDFIESGLGWKYDAARVLRAM
                     RDRETLAVVACEGAKGAPSSARSAVAGFAIMEFGDERAHLMLLAVRPSHRRIGIGQRM
                     LDWLLHSARVAGMASIHLELRAGNDAARRFYRAMGFYETVLVPGYYRSGEGRKEGALR
                     MLRVLRSPGPVPYTWRPPRTEDT"
     gene            2845..4227
                     /locus_tag="VFX94_05325"
     CDS             2845..4227
                     /locus_tag="VFX94_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5767637.1"
                     /translation="MRTRKKPNLVVVLVDDLRFDETGASGHPYMRTPNIDRLAHEGAR
                     FANAFHTTPLCSPNRASIVTGQYASRHGIIDNVGREAMSHRLANYHLELQRLGYETAH
                     IGKWHMGNDAAPRPGYDTWISFRGQGTITDPVLWENGNEKQVRGYVTDLLSERAAQFV
                     ARRRDKPFALFLAHKAVHPDVQQKQDGTIDLATMQGYVLPERHKDLYRGATYPPRPNV
                     RPIERVLEDKPAWRDVFELRSRPESRPFLEGLQSGTQEEIRRRAAMMASVDEGIGALL
                     AELEKQGSLDDTLLLFLGDNGFFFGEHGLGAERRFAYEEAIRTAFFVRYPRLVKPGSV
                     IEPMVLALDIAPSLIELAGGKPGPQIQGRSFVPLLRRRAGKWRKSFLIEYYNESAWPW
                     IVGMSYKAVRTERAKLIHWVHKEGMDELYDLERDPYEIRNIIRKPSYDKVRTRLYREL
                     RKLVAEAVGL"
     gene            4251..5477
                     /locus_tag="VFX94_05330"
     CDS             4251..5477
                     /locus_tag="VFX94_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX5767638.1"
                     /translation="MKNDDVASLSRDRTVDLSDEIKSRSHAEAAQKLAHLGGDMIARE
                     LMHLSPGFAQDVLGALPNAARERAVSAAPSDVALQWQRNSMYDPGSVGRMMEPVAAAF
                     PPERNVGEVIEELRELVTRTLITYVYVVDEDDQLLGIVTMRDLLFSTRDRTLEEVMLR
                     GAFALHAAMPMHDAMRLVLDRHYPVYPVIDAERRLIGLVRGQTMFEAQAYEISLQAGT
                     MVGLEKEERLGTPWAHSLKLRHPWLQLNLLTAFLAAAVVGVFQDTIDRLVILALFLPV
                     LAGQSGNTGCQALAVTLRGMTLGELKPGSERPLMAKETWVGCLNGIGVGIAAAVGMYI
                     TASSQNQAEPWMLALVVFLAMIASCMASGISGAIIPLTLKRFGFDPATASSIFLTTAT
                     DVVSMGVLLGLATILV"
     gene            complement(5484..>5833)
                     /locus_tag="VFX94_05335"
     CDS             complement(5484..>5833)
                     /locus_tag="VFX94_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5767639.1"
                     /translation="LRRARGAIVNISSTRALQSEPDTEAYAASKAGLVGLTHALAMSL
                     GPEVRVNCVSPGWIAHQSVRKKDATQHPVGRVGRDEDIAELVAYLLSDAAGFVTGQNF
                     VVDGGMTRKMIYV"
BASE COUNT          982 a         1936 c         1959 g          956 t
ORIGIN      
        1 cgagcagcag gaacggcgaa agctcgggct ggcgctggta gtcgagcacc gtgtggaccg
       61 ggaaaccgtc tccaacccag tgcccttcgg gcttgcgatg aatacgcaga atgtttttca
      121 tgttcgtaat atgcaggcgg ctaggatagg gataaagtag ccttcaggta aaggatcatc
      181 ccggaacttg ggacaatgga caggctaggc gcgatccagg tgttcgcgca agttgttgaa
      241 tcagggagct ttgccaaggc ggccgagcgg ctcgggctgt cgacttcggc gacgtcccgc
      301 catgtcgcgg acctggaggc ccacctgcag acccgccttc tcaaccgaac gacacggcgc
      361 gtaagcctga cggaaagcgg gcgcgccttc tatgagcgcg cggtgcagct cctcgccgac
      421 ctcgaggagg ccgagcagga agctgcgcgc gccgccgtcg tgccgcgcgg caccatccgc
      481 ctgacgacgt cggtgaattt cggcgtgcgc cacctcgcgc cggcgatcgc cgagttcctc
      541 ggccggcacg ccgaggtgcg cttcgacgtt tcactttccg atcgcgtggt cgacctcatc
      601 gaagagggct tcgatctggc cgtgcgcctc ggcacgacgg gctcggagaa cgtggtcgcg
      661 cgaaagctcg gggaaacgcg gctcgtgccg tgcgcctcgc cgcagtacct atcggcgcat
      721 cccgcgccga aggccccgga ggacctggcc gcgcacaact gcttcaccta cgagtacatc
      781 acgccgcgta acgtgtggcg ttttcgcgat gcggccggag gcgagcgcgc ggtacggatc
      841 gcgggcacgc tgcattcgaa caacggagac cttctggcgg aaatcgcggc gcgcgggtgc
      901 ggcatcgtgt tcgagccggc tttcatcgtc ggtcctgatg tgcgtgcggg gcgcctcgtt
      961 ccgctgctcc aggatttcat gccgccgccg gtgccgatct acgccgtcta cccgagccga
     1021 aagcacttat cggccaaagt acgcgcgttt gtcgacttcc tggtcacgcg cttcgaggac
     1081 gcgcccgact ggagcgcggc gtgaaggcgc cggcgcttgc cgcattgctt cttctcgctt
     1141 taagtagcgc gcatgccgct gactggctca tcgccgcttc ggagccgcgc gcggatgccg
     1201 gtggatattt ggaaatcgtg gtggtcgcgc cgccgggaga gacgccgccg ccggagctgc
     1261 gcgtgcggat tcaggccgac gtcgccgaga tcgacgtcga gttcaaggcg accgcgccgg
     1321 ccgacggcgc ccggcgcaac tacgcgggca cgatgccggc gttcgcggcc ggcacggtca
     1381 cgcttcagct cgccgagctg ccgtccaaca ggctcgtgct catcgccgtg cggcgcgacg
     1441 cgctacaggc actgacccag ccgctcgccg gcggcgctga ggcgccgctc tccgagaacg
     1501 agcccatgta cttcatcgcc ggctcgcgcg gcccgaccac cgcgcgcttt cagatctcgt
     1561 tcaagtaccg cctgttcgac accacctcgg gcttcggcca gggcaagccc tggctcacgg
     1621 gcctgtatct gggctacacg caaaactcgc tgtgggacct gtcgagcgag tccgagccgt
     1681 tccgcgacac gggctaccgg ccgtcgctat tctggcaatg gtagcgcgcc ggcgagcgga
     1741 aacttttcga tggcgtgcgc ctggggctcg agcacgagtc gaacggagaa gagggcgagc
     1801 gctcgcgctc gctcaacatc atgttcgtgc gcccggaatg gatctggcgc ctgcgcagcg
     1861 gcgcgagctt cgagttcacg cccaggatct acggttacct cgacaaggag gagaaccccg
     1921 atatcgacga gtaccgtggc tacgccgact ggcgcgtgcg ctacgactcg gggaacctgt
     1981 ggctcaccac cgcgctagct cgcgtcggtg ccgcgggaaa gggaagcctg cagctcgatt
     2041 tcgcgcgccg cacgcgcgac ttcaagttcg gcggcgtgag cggctacctg tacgcacaat
     2101 acttcaacgg ctacggcgag gacatcatcg actacaacgt gaaacgcagg gcccagttca
     2161 gggtcggctt cgcgatcgtg ccgtagaaaa ggggtcagga accttttccg ctgcttcgcg
     2221 tagcgcgcca gggaaaaggt tctgacccct tttctaggtg tcctcggtgc gcggcgggcg
     2281 ccaggtgtac ggcaccgggc cgggggagcg cagcacgcgc agcatgcgca gcgcgccttc
     2341 cttgcggcct tcgccgctgc ggtagtagcc gggcacgagc accgtctcgt agaagcccat
     2401 cgcgcgatag aagcggcgcg cagcatcgtt gccggcacgc agctcgaggt ggatgctcgc
     2461 catgccggcg acgcgggccg agtggagcag ccagtcgagc atgcgctgcc cgatgccgat
     2521 ccgccggtgc gacgggcgca ccgcaagcag catgaggtgc gcgcgctcgt cgccgaattc
     2581 catgatggcg aagccggcga ccgcgctgcg cgcggagctg ggcgcgcctt tcgcgccttc
     2641 gcacgccacc accgcgagcg tctcgcggtc gcgcatcgcg cgcagcacgc gtgccgcgtc
     2701 gtatttccag ccgagcccgg actcgatgaa gtcgcgcgac atcgttgcga tcgcttgtgc
     2761 gtcgcgcggc tcggcgagcc ggatggcgat ggcgcggggc gtcatggcgg gcattgtgcc
     2821 atgagaaaat ggccgctgtc ccctatgaga acgagaaaaa agcccaatct cgtcgtcgtt
     2881 ttggtggatg acctgcgctt cgatgagacc ggcgcgtcgg gacatcccta catgaggacg
     2941 ccgaacatag accgcctggc gcacgagggc gcgcgcttcg cgaatgcctt ccacaccacg
     3001 ccgctgtgct cgcccaaccg cgcctccatc gtcaccgggc agtacgcctc ccgccacggc
     3061 atcatcgaca atgtcgggcg cgaggcgatg agccaccgcc tcgccaacta tcacctcgag
     3121 ctgcagcgcc tgggctacga gaccgcgcac atcggcaaat ggcacatggg caacgatgcg
     3181 gcgccgcgtc ccggctacga cacctggatc agctttcgcg gccaggggac catcaccgac
     3241 ccggtgctct gggagaacgg caacgaaaag caggtgcgcg gctacgtgac ggacctcttg
     3301 agcgagcgcg ccgcgcagtt cgtggcccgg cgccgcgaca agccgttcgc gctttttctc
     3361 gcgcacaagg cggtgcaccc cgacgtgcag cagaagcagg acggcaccat cgacctggcg
     3421 acgatgcagg gctatgttct gcccgagcgc cacaaggatc tctaccgcgg cgcaacgtat
     3481 ccgccgcgcc ctaacgtaag accgatagag cgggttcttg aagacaaacc ggcctggcgc
     3541 gacgtgttcg agttacgcag ccgccccgag agccggccgt tcctcgaggg gctgcaaagc
     3601 ggcacacagg aagagatccg ccggcgcgcc gcgatgatgg cctcggtgga cgagggaata
     3661 ggcgcgctgc ttgcggagct cgagaagcag gggagcctcg atgacacgtt gctccttttc
     3721 ctgggcgaca acggcttttt cttcggcgag cacggcctcg gcgccgagcg gcgcttcgcg
     3781 tacgaggagg caatccgcac ggcattcttc gtccgttacc ccaggctcgt gaagccgggc
     3841 agcgtgatcg agccgatggt gcttgcgctc gatatcgcgc ccagcctgat cgagctcgcc
     3901 ggcggcaagc ccgggccgca gattcagggc cgctctttcg taccgctgct gcgccgcagg
     3961 gcggggaaat ggcgcaaatc gttcctcatc gagtactaca acgagtcggc gtggccgtgg
     4021 atcgtcggca tgtcctacaa ggccgtgcgc accgagcgcg cgaagctgat ccactgggtc
     4081 cacaaggagg gtatggacga gctttacgat ctcgaacgcg atccctatga aatccgcaac
     4141 atcatccgca aaccctccta cgataaagtg cgcacgcggc tataccgcga gctgaggaag
     4201 ctcgtcgcgg aggcagtcgg gttgtaggca gggaaccacc ggggggtggc atgaagaacg
     4261 atgacgtggc aagcctttcc agggatcgca cggtagacct ttccgacgag atcaagtcgc
     4321 gctcgcacgc ggaggcggcg cagaagctcg cgcacctggg cggcgacatg attgcccggg
     4381 agctgatgca tctcagcccg gggttcgcgc aggacgtcct cggcgcgctg cccaacgcag
     4441 cgcgcgagcg cgccgtctcg gcggcgccgt ccgacgtagc gctccagtgg cagcgcaact
     4501 cgatgtacga cccgggcagc gtcgggcgca tgatggagcc ggtggcggcc gccttcccgc
     4561 ccgagcgcaa tgtcggcgaa gtgatcgagg aactgcgcga gctcgtcacg cgcaccctca
     4621 tcacttacgt atatgtcgtc gacgaggacg atcaactcct cgggatcgtg acgatgcgcg
     4681 acctgctctt cagcacgcga gatcgcacgc tcgaggaagt catgctgcgc ggcgcgtttg
     4741 cgctgcacgc ggcgatgccg atgcacgacg cgatgcggct cgtgctcgac cggcattacc
     4801 cggtctaccc cgtgatcgac gccgagcgcc gcctgatcgg cctggtgcgc ggccagacga
     4861 tgttcgaggc gcaggcgtac gagatttcct tgcaggcggg cacgatggtc ggcctggaga
     4921 aggaggagcg cctcggcacg ccgtgggcgc acagcctcaa gctgcgccac ccctggctgc
     4981 agcttaacct cctcaccgcg ttcctcgccg cggcggtggt gggcgttttc caggacacga
     5041 tcgaccggct ggtgatactg gctctttttc tccccgtgct cgccggccag tcgggcaaca
     5101 ccggctgcca ggcgcttgcg gtcaccctcc gcggcatgac gctcggggag ctgaaaccgg
     5161 gcagcgagcg gccgctgatg gcgaaggaaa cctgggtcgg ctgcctgaac ggcatcggcg
     5221 tcggcatcgc cgccgcggtc ggcatgtaca tcaccgcgag cagccagaac caggccgagc
     5281 cttggatgct ggcgctagtc gtgttccttg ccatgatcgc ctcctgcatg gcaagcggca
     5341 tctcgggcgc catcatcccg ctcaccctca aacgcttcgg cttcgacccg gccaccgcgt
     5401 cgagcatctt cctcaccacc gcgaccgacg tggtgagcat gggcgtgctg ctcggcctgg
     5461 caacaatact ggtttaaaag ggtctacacg tagatcatct tgcgcgtcat gccgccgtcg
     5521 accacgaagt tctgcccggt gacgaagccc gcggcatccg atagcaggta ggcgacgagc
     5581 tcggcgatat cctcgtcgcg tccgacgcgg ccgaccgggt gctgggtcgc gtctttcttg
     5641 cgcacgctct ggtgcgcgat ccagcccggg ctcacgcagt tgacgcgcac ctcggggccc
     5701 aggctcatcg cgagtgcgtg cgtcagcccg acgagaccgg ccttggaggc ggcgtaggcc
     5761 tcggtgtcgg gctccgactg cagcgcgcgg gtcgacgaga tgttcacgat ggccccgcgc
     5821 gcgcggcgca ggt
//
LOCUS       DASUNT010000269         2515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_212253, whole genome shotgun sequence.
ACCESSION   DASUNT010000269 DASUNT010000000
VERSION     DASUNT010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212253"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..820
                     /gene="plsY"
                     /locus_tag="VFX94_05340"
     CDS             200..820
                     /gene="plsY"
                     /locus_tag="VFX94_05340"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX5767640.1"
                     /translation="MHTATPVLVLLAAYCLGSVSFAIVVSHVMRLPDPRSYGSKNPGA
                     TNVLRTGRKAAAVLTLAGDGAKGWAAVWLASFFTAELALAGLAAFLGHLFPVFHRFRG
                     GKGVATAAGVLLGLDWRLGLATFAVWLVVVAVLRYASAASLAAALCAPFATGLLLGWK
                     SALVVGVMALLLLWRHRANVARLLAGTEPRLGEKSASPADPSAASQ"
     gene            complement(786..1760)
                     /gene="tsaD"
                     /locus_tag="VFX94_05345"
     CDS             complement(786..1760)
                     /gene="tsaD"
                     /locus_tag="VFX94_05345"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX5767641.1"
                     /translation="MLILGIETSCDDTGVALYDSAAHTILAHAVHTQASMHEAYGGVV
                     PELASRDHVRRLVPLTRKVLGSHAIDGIGYTEGPGLAGALLVGASFAHGLAVSLGVPA
                     VGIHHLEGHLLSPLLSARRPDFPFLALLVSGGHTQLMRVDGIGRYQLLGETQDDAAGE
                     AFDKAAQLLGRGYPGGPALSRLAERGAPGRYRLPRPMLGAGLDFSFSGLKTAVLMLVK
                     NEQPEPADVARAFVDAIVEVLVAKCARAVETTGLTDLVVAGGVGANAQLRAALDAEAA
                     RRGFDVFYPEPELCTDNGAMIAFAAAHRLKDAAREHSFTVKPRWDLQG"
     gene            1807..2019
                     /gene="rpsU"
                     /locus_tag="VFX94_05350"
     CDS             1807..2019
                     /gene="rpsU"
                     /locus_tag="VFX94_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX5767642.1"
                     /translation="MPTVRVKENEPFEVAMRRFKRTVEKTGLLTELRSREYYEKPTAE
                     RKRKLAAAVKRHHKRLRSQMLPQKLY"
     gene            2050..2496
                     /locus_tag="VFX94_05355"
     CDS             2050..2496
                     /locus_tag="VFX94_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX5767643.1"
                     /translation="MSLKPKITEDMKAAMRAKDSARLSAIRLLLAAIKQREVDERKEL
                     TDAEVASVIEKMIKQRRESIAQYDKAARKDLADAERFELDVLTGYLPAQMNEAALSQA
                     VDAVISEVKPAGVKDMGRVMALLKQRLAGKADMAKVSALVKTKLSG"
BASE COUNT          446 a          820 c          834 g          415 t
ORIGIN      
        1 ccacctgctc gtagtcgacg gtgtcggcca ctttgtcgct gctgaaaacc gcgttgcccg
       61 ggacgccgat ctcgaggtcg accgatatcg tctgcggcgc atggcgctcg cgccggtgga
      121 tgccgacgag cgcttcgacg cgcaggtcgc ggatgacgat cgcgtccatg accggcacga
      181 ttctatagaa tcgcccgcga tgcacaccgc gacgccggtt ctcgtgcttc tcgccgcgta
      241 ttgcctcggc tccgtgtctt tcgccatcgt ggtaagccac gtgatgcgcc tgcccgatcc
      301 gcgcagctac ggatcgaaga atccgggcgc cacgaacgtg ctgcgcaccg ggcgaaaggc
      361 cgccgcggtg ctcacgctcg ccggcgacgg cgccaagggc tgggcggcgg tatggctcgc
      421 ctcgttcttc accgccgaat tggcgctcgc ggggttggct gcgttcctcg ggcatctctt
      481 cccggtcttc caccggttcc gcggcggcaa gggtgtcgcc acggcggccg gcgtgctgct
      541 ggggctcgat tggcggcttg gcctggcaac gttcgcggtg tggctcgtgg tcgtcgctgt
      601 actgcgctac gcctcggcag catcacttgc cgctgcgctc tgcgcgccgt ttgcgacggg
      661 gctgctgctc ggctggaaga gcgctctcgt ggtgggcgtc atggcgcttc tgctcttatg
      721 gcggcacagg gccaacgtgg cgcggcttct tgccggcacc gagccccggc tcggcgagaa
      781 aagcgctagc cctgcagatc ccagcgcggc ttcacagtga acgaatgctc gcgcgctgcg
      841 tccttcagcc ggtgggcggc tgcgaaggcg atcatggcgc cgttgtcggt gcagagctcg
      901 ggctccgggt agaacacgtc gaacccccgg cgcgccgctt cagcgtcgag cgccgcgcga
      961 agctgcgcgt tcgcgccgac gccaccggca acgacaagat cggtaagccc tgtcgtttcc
     1021 accgcgcgcg cgcacttcgc gaccagcact tcgacgatcg cgtcgacgaa ggcgcgcgcc
     1081 acgtcggcgg gttcgggctg ttcgttcttc accagcatca gtaccgcggt cttgaggccc
     1141 gagaagctga agtcgagccc ggcgccgagc atcggccggg gcaggcggta gcgccccggc
     1201 gctccccgct cggctagcct ggaaagcgca ggacctccgg gataaccccg gccgaggagt
     1261 tgcgccgcct tgtcgaacgc ctcgccggcg gcgtcgtcct gcgtctcgcc gagcagttga
     1321 tagcgcccga taccgtccac ccgcatgagc tgggtatggc cgccggaaac gaggagcgcg
     1381 aggaacggga aatcggggcg ccgcgcggag agcagcggcg aaagcagatg gccttccagg
     1441 tggtgaattc ccacggccgg cacccccaac gagacagcaa gaccgtgcgc gaaagaagcg
     1501 cccactaaca aagcgccggc gagacccggc ccctcggtat agccgatgcc gtcgatggcg
     1561 tgactgccga ggactttcct cgtaagcggc acgagcctgc ggacatgatc ccgcgaggcg
     1621 agctcgggaa ccacccctcc atacgcttca tgcatggagg cctgcgtatg tacagcgtga
     1681 gcaaggatcg tgtgcgcggc gctgtcgtac agcgcgacgc cggtatcgtc gcaagaagtc
     1741 tcgatgccga ggatgagcac gaggcgattc tgatatacaa ttcagcccct tttccagcag
     1801 gggtttatgc cgacagtacg tgtgaaggaa aacgagccgt tcgaagtggc gatgcgccgc
     1861 ttcaagcgca ccgtggagaa gactggcctt ctcaccgagc tgcgctcccg cgagtactac
     1921 gagaagccga ccgcagaacg caagcgcaag cttgccgcgg ctgtgaagcg acaccacaag
     1981 cgcctgcgca gccagatgct cccgcaaaag ctgtactgac gtaagccgcg tgcttctagc
     2041 ggccgatcca tctctctcaa gcccaaaatc accgaagaca tgaaggccgc catgcgggcg
     2101 aaggattcgg ctcgcttgtc cgccattcga ttgcttcttg cagcaataaa gcagcgcgaa
     2161 gtcgacgagc gcaaggagct caccgacgcc gaggtcgcct ccgtaatcga gaagatgatc
     2221 aagcagcgga gggagtccat cgcccagtac gacaaggcgg cgcgcaagga cctggcggac
     2281 gccgagcggt tcgagctcga cgtgctgacg ggctatctgc cggcgcagat gaacgaagct
     2341 gcgctttccc aggccgtcga tgccgtaatt tccgaagtga aaccggcagg cgtcaaggac
     2401 atgggcaggg taatggcgct gctcaagcag cggctcgccg gcaaggcgga tatggccaag
     2461 gtatccgccc tagtcaagac caagctttca ggatgacaat cggggcgtga tcccc
//
LOCUS       DASUNT010000270         3318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1264908, whole genome shotgun sequence.
ACCESSION   DASUNT010000270 DASUNT010000000
VERSION     DASUNT010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264908"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VFX94_05360"
     CDS             complement(<1..687)
                     /locus_tag="VFX94_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767644.1"
                     /translation="MGVTVPEWLLTAVIAVTVFTVMFGIGLGITPGELRRVWLPPGPM
                     LRGLFSVLVAVPMLAVAIAQAFDLSRAAEIGIVLMAISPGAPVALRRSLAAGAHAAFA
                     PGLQISVALLAVASMPLWIALLNEFYAGQARIDPRDVMRQVFIAQLLPLGIGIALRHF
                     AATLAGRIEPAVRRAGTVLLIAAVVLVLAELWRPLYEAGVATIAAMVLTTAAALAVGH
                     LLGGPAPTTRT"
     gene            758..1000
                     /locus_tag="VFX94_05365"
     CDS             758..1000
                     /locus_tag="VFX94_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767645.1"
                     /translation="MRLLPALVTLAFAALVGSGCAGPTVQCQGTDWHELGQRHGILDA
                     RGEAERYASACGNAFDRARYQEGFQDGFSRRARPMV"
     gene            1034..1639
                     /locus_tag="VFX94_05370"
     CDS             1034..1639
                     /locus_tag="VFX94_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HEX5767646.1"
                     /translation="MGFRPSKQEGRMGLMLKQVAAGWLFALAALPARAAFLSGEALDT
                     AADIIALVVIFVVPAIVIVLFWIVHVLPEKIAHKRHHPQRDAITTLCLLSLVFGGLLW
                     PLAWIWAYTKPIGYRLAYGTDKHDDYYDEMAEKQREGKLVREEAFHLREELEAMEARG
                     ALPPKLKGLKDELARLRLADAEERAERMTAPKERPAKDKAA"
     gene            1640..2863
                     /locus_tag="VFX94_05375"
     CDS             1640..2863
                     /locus_tag="VFX94_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX5767647.1"
                     /translation="MEVLLLGIYSFFVWLIFIKLKWLPWNTATQVTVVIIPIVALGAT
                     ILLLNIYAPSSPDVRVYKYTIPIVSQVRGRVIEVAVEEGNRLVKKGEVLFKIDPTPYE
                     LEVNTLEAQLANAQGSQREVQESQKGARARIDETRGAIVAATSRTREVSAKLQLARTR
                     VEQYRELVNTGAGNRFDLERAMADVQELESQLAAARSAEVQARASEGQAIASERQIQQ
                     KLGATSKGEYAQVAQIRAQLENAKWQLDQTTTRSPCDCYVVNLQLRPGGFVAGLPVNP
                     VMTLIEADGQVVALYHQNELHQVKPGNEVEFALKTIPGKIIKGKVDSIVWAQGQGQLL
                     PTGTIPMSGVVAQPPGRYAVKFNIADRDRELFLAAGAAGDAAIYTDGLHAIHIIRKVI
                     LRVGSYINYLIPKLH"
     gene            2873..>3318
                     /locus_tag="VFX94_05380"
     CDS             2873..>3318
                     /locus_tag="VFX94_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5767648.1"
                     /translation="MARRLGLVVIVAALAGCALKSPPERAEVAKQALPNLQVPAAWNA
                     QGGAAGVIADKWLAGFNDPQLDALVQEALANNPDLQVAAGRVEQAAAFAKVAGATLYP
                     QVNALARGGGALSGDSSGLEGAGIFASWEIDLWGRVRSGRAAARDQ"
BASE COUNT          582 a         1103 c         1165 g          468 t
ORIGIN      
        1 ggtgcgggtc gttggagcag ggccgccgag caggtggccc acggctagcg ccgctgcggt
       61 ggtgagcacc atggcggcga tggtcgccac gcccgcctcg tagagcggcc gccatagctc
      121 ggcgagcacc aggaccaccg cggcaatgag cagcacggtc ccggcgcgcc tgaccgccgg
      181 ctcgatccgc cctgcgagcg tcgccgcgaa gtgacgcaac gcgatgccga tgccgagcgg
      241 aaggagctgt gcgatgaaca cctggcgcat tacatcgcgc ggatcgatgc gcgcctggcc
      301 ggcgtagaac tcgttgagca gcgcgatcca gagcggcatc gacgccacgg cgagcagcgc
      361 caccgatatc tgcaatcccg gagcgaaagc ggcatgcgcg ccggcagcga gcgagcgccg
      421 cagcgccacc ggcgcgccgg gcgagatcgc catgagcacg atgccgatct ccgccgcgcg
      481 cgacagatcg aacgcctggg cgatggccac cgccagcatg gggactgcga ccagcacgga
      541 aaagagaccg cgcagcatcg ggccgggcgg cagccacacg cggcgcagct cgcccggcgt
      601 tatgccgagc ccgataccga acatgacggt gaagacggtg accgcgatga ccgcggtcag
      661 cagccactcc gggaccgtca cccccatggc ggcaagctaa gcgccggaaa accgggccga
      721 aatcctacct aggtgcgatt ctgggtaagc ttgcgggatg cggctcctgc cggctctcgt
      781 cacgctcgct ttcgcagcgc tcgtgggctc gggctgcgct ggccccacgg tccaatgcca
      841 aggcaccgac tggcatgagc tcggccagcg ccatgggatc ctggatgcgc ggggagaggc
      901 ggagcgctat gcctctgcct gcggcaacgc cttcgaccgt gcacgctacc aggagggctt
      961 ccaggacggc ttctcgcgcc gcgccaggcc gatggtttag gaaccggaaa cacctcgcta
     1021 aaatcgtacc ttggtgggat tccggccctc gaagcaggaa ggcaggatgg gactcatgct
     1081 gaagcaggtt gcagccggct ggctgttcgc gctcgccgcc ttgccggcgc gcgcggcgtt
     1141 tctttcgggc gaggcgctcg acacggcggc cgacatcatc gcgctggtcg tgattttcgt
     1201 cgtgccggcc atcgtgatcg tgctcttctg gatcgtccat gtgctgcccg agaagatcgc
     1261 ccataagcgt caccatccgc agcgcgacgc cattaccacg ctttgcctgc tgtcgctcgt
     1321 cttcggcggc ctgctctggc ctctcgcatg gatctgggcc tacaccaagc cgatcggcta
     1381 ccgcctcgcg tacggcaccg acaagcacga cgactactac gacgagatgg cggagaagca
     1441 gcgcgagggc aagctggtgc gcgaggaggc gttccacctg cgcgaggaac tggaggcgat
     1501 ggaggcgcgc ggcgccctgc cgccgaagct gaagggactg aaggacgagc tcgcgcgcct
     1561 gcggctcgcc gacgcagagg aacgcgcgga acgcatgacg gcgccgaagg aaaggccggc
     1621 gaaagacaag gcggcgtaga tggaagtcct ccttctcggc atctactcgt tcttcgtttg
     1681 gctgatcttc atcaagctga agtggctgcc gtggaacacc gcgacgcagg tcaccgtggt
     1741 catcatcccg attgtcgcgc tcggcgccac cattctgctg ctcaacatct acgcgccttc
     1801 gtcgccggac gtgcgcgtct acaagtacac catcccgatc gtgtcccagg tacgcgggcg
     1861 cgtgatcgag gtggcggtcg aggaaggcaa ccgcctggtg aagaagggcg aggtgctgtt
     1921 caagatcgac ccgacgccct acgagctcga ggtgaacacc ctggaagccc agctcgcgaa
     1981 cgcacagggc tcgcagcgcg aggtccagga gtcccagaag ggagcgcgcg ctcggatcga
     2041 cgagacgcgc ggggcgatcg tggcggcgac gagccgcacg cgcgaggtga gcgcgaagct
     2101 ccagctcgcg cgcacccgcg tggagcagta ccgggaactg gtgaacaccg gcgccggcaa
     2161 ccgcttcgac ctcgagcgcg caatggccga cgtgcaggag ctcgaaagcc agctcgccgc
     2221 ggcgcgcagc gccgaggtgc aggcgcgcgc ctccgagggg caggcgattg cgagcgagcg
     2281 ccagatccag cagaagctcg gcgcgacgtc gaagggcgag tacgcgcagg tcgcgcagat
     2341 ccgcgcccag ctcgagaacg cgaagtggca gctcgaccag accacgacgc gctcgccgtg
     2401 cgactgctat gtcgtcaacc tgcagctgcg ccccggcgga ttcgtcgcgg gactcccggt
     2461 caacccggtg atgacgctga tcgaagccga cgggcaggtg gtcgcgctct accaccagaa
     2521 cgagctgcac caggtgaagc cgggcaacga ggtagagttc gcgctgaaga cgattccggg
     2581 gaagatcatc aagggcaagg tcgattcgat cgtgtgggcg cagggccagg gccagcttct
     2641 gccgaccggg accattccga tgtccggtgt cgtggcgcag cctcccggcc gctacgcggt
     2701 gaaattcaac atcgccgaca gggaccgcga gctcttcctc gccgccggcg cggcgggcga
     2761 cgccgcgatc tacaccgatg gcctgcacgc gatccacatc atccgcaagg tgatcctgcg
     2821 ggtgggctcc tacatcaact acctcatccc caagcttcac tgaacgatgg gcgtggcgcg
     2881 ccggctcggc ctcgtcgtga tcgtcgccgc tctcgcaggc tgtgcgctca agtcgccgcc
     2941 cgagcgtgcc gaagtcgcca agcaggcgct gccgaacctg caggtgccgg ccgcgtggaa
     3001 cgcgcagggc ggcgccgccg gcgtgattgc ggacaagtgg cttgcgggct tcaacgaccc
     3061 gcagctcgac gcgctggtgc aggaggcgct cgccaacaac cctgacctcc aggttgcggc
     3121 ggggcgcgtc gagcaggctg cggcgttcgc caaggtggca ggggcgacgc tgtatccgca
     3181 ggtgaacgcg ctcgcgcgcg gcggcggcgc gctgagcggc gactcgagcg ggctcgaagg
     3241 tgccgggata tttgcgagct gggagatcga tctctggggt cgggtgcgct cgggccgcgc
     3301 cgcggcgcgc gaccagca
//
LOCUS       DASUNT010000271        11616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_125043, whole genome shotgun sequence.
ACCESSION   DASUNT010000271 DASUNT010000000
VERSION     DASUNT010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11616
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125043"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..620
                     /locus_tag="VFX94_05385"
     CDS             87..620
                     /locus_tag="VFX94_05385"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767649.1"
                     /translation="MLNTARSVLFVSCAVLAVTAAAEGPRIDSQTSGATRGQPAGGAT
                     GETAGAMQGPMAPDRNAVEVAPAGMQPYLTDVRGRALYMLEGENAGKVRCDAACARVW
                     PPLRDEANLAGKGLKKTLVGSRKRADGSSQVTYNGHPLYYYVEDRGAGKATGQVHDRW
                     GEWYLVTPQGGKFEKRS"
     gene            complement(617..1321)
                     /locus_tag="VFX94_05390"
     CDS             complement(617..1321)
                     /locus_tag="VFX94_05390"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5767650.1"
                     /translation="MKQKMMQDLKGDAILARNGKLGSLKDVYFDDERWTVCYFVVDTG
                     GWFSGREALISPHLVRAHEGRARELQVELTREELERAPGPAEHPPASRLHEAARSADS
                     AYPAYRSAPYLWVGTPSPLAVPPPQLERDPGDARNEAAVQAQKRAEQSHLRSAAELVG
                     YHIHARDGDLGHVEDFVVESDTWTITGMVVDTRNWLPGKKVLVPPSAVESVDWHARRV
                     AVRRTRAELERSPEPR"
     gene            complement(1330..1605)
                     /locus_tag="VFX94_05395"
     CDS             complement(1330..1605)
                     /locus_tag="VFX94_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767651.1"
                     /translation="MPEGKLLVRASYLCAALALGLLAIHFNAELLPGWTRGLALLAGA
                     LALVGAGLAVLYLGRHGWTRLRHGPGQVALALNSVLAIAFAFYVDLT"
     gene            complement(1608..1880)
                     /locus_tag="VFX94_05400"
     CDS             complement(1608..1880)
                     /locus_tag="VFX94_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767652.1"
                     /translation="MHALTIFALTLLLAAHMRGALPGAQRALAHVAILIALGVALGTA
                     WEIAEWLFDALTYGNAIRGKTDTMTDLLADTVGAALAAWLALQKVG"
     gene            complement(2016..2252)
                     /locus_tag="VFX94_05405"
     CDS             complement(2016..2252)
                     /locus_tag="VFX94_05405"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5767653.1"
                     /translation="MIDDAALTAKVKQQLVEEDKVNAASVNVTTYRGVVQLSGFVESD
                     EAKRRAENAAKDVEGVRSVQNDLRIAPSASGKTR"
     gene            2504..2716
                     /locus_tag="VFX94_05410"
     CDS             2504..2716
                     /locus_tag="VFX94_05410"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEX5767654.1"
                     /translation="MRIQPSPFRVQKFLAGLRYPARKQQVLERARSRGADAQLIRLLL
                     LLPERDYESPISLSCEVGRQAERSRN"
     gene            2725..3327
                     /locus_tag="VFX94_05415"
     CDS             2725..3327
                     /locus_tag="VFX94_05415"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5767655.1"
                     /translation="MRRLTLAAALSALALSLPAEAGAVAAGVLIGRPIQDLSGQVLGT
                     VEDMIVDVRDARVLYIIVDARERDFTLPIRALREHEGGVRLDMSDAGEIARTDDPRFR
                     RAARLIGQPLMNPNGGRIGTIADIEFDPAAGRVERVVVDVDNGKANFPPSVLAHGRFP
                     PLTRWQAEHPPSDFSGDPGYVRRDPSPERERLHDHRWPRN"
     gene            complement(3324..3848)
                     /locus_tag="VFX94_05420"
     CDS             complement(3324..3848)
                     /locus_tag="VFX94_05420"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEX5767656.1"
                     /translation="MRALAAAAFVIAGAAGAESVPGALRFGEIRLEAAICGVRSTLWI
                     EHYVAALYLPERRSALEELLDPTSAKALYVRILNDDHLPLRIPREWREPIVRHLSVDD
                     LARARAAYRRLRPGDELFIAYLPDAGVRLVINDRLIASSSGHALVEAVLGTWTEGKPL
                     GEKLREVTADNTCR"
     gene            complement(3855..4175)
                     /locus_tag="VFX94_05425"
     CDS             complement(3855..4175)
                     /locus_tag="VFX94_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767657.1"
                     /translation="MRIVCVCSALLATAASALLAADPVTEWRDWPPDPQWAEQRPWAE
                     APRVIPRAMFAALDSNYDFSLSREEAAASPPVEANFGPADLDRNGRLTPLEFNNIALA
                     LAAD"
     gene            complement(4179..4358)
                     /locus_tag="VFX94_05430"
     CDS             complement(4179..4358)
                     /locus_tag="VFX94_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767658.1"
                     /translation="MAAVDYLLSARKNLDALAELLADAAAEQKAVADIADEALVMVND
                     VRDVLDTMGDEEGTE"
     gene            4426..4650
                     /locus_tag="VFX94_05435"
     CDS             4426..4650
                     /locus_tag="VFX94_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767659.1"
                     /translation="MRYAVIDPAATIESASAALRACDADALLVIGARPQPASPVLSAR
                     DIVTRVVAVGLDPRVVTVSDLLRVMADRPL"
     gene            complement(4896..5183)
                     /locus_tag="VFX94_05440"
     CDS             complement(4896..5183)
                     /locus_tag="VFX94_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767660.1"
                     /translation="MVTLAMAPHEVLGDEIRQFSGTITARWVAQTSQGAQQFARLHTA
                     DGGSFVLDLGAPGGEGTIQKGDEVVLTAEARQVDGEPVLRVKVITERIPAR"
     gene            complement(5250..5924)
                     /locus_tag="VFX94_05445"
     CDS             complement(5250..5924)
                     /locus_tag="VFX94_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEX5767661.1"
                     /translation="MEEPDRAQILRELQEWLEKPMIALSFVWLVLLVVELAWGLNRAL
                     WILGQAIWVLFIAEFALSLALAPERVRYLRENWLKVLALAAPALRVLRIARLARFARA
                     SRGLRLLRVVASVNRAMAALGAAMQRRGFGYVLLLTMVVVFAGAAGMYAFERDAPDGG
                     FDSFATALWWTAMLVSTMGSEYWPKTPDGRALCLALAIYGFAIFGYVTAALATYFIGR
                     DREQSL"
     gene            complement(5969..6130)
                     /locus_tag="VFX94_05450"
     CDS             complement(5969..6130)
                     /locus_tag="VFX94_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767662.1"
                     /translation="MIGRLLAAALAVCLLAGFIAPVALKLVDDYALLAVLAVGFVLML
                     IDLAQSLRS"
     gene            complement(6127..7470)
                     /locus_tag="VFX94_05455"
     CDS             complement(6127..7470)
                     /locus_tag="VFX94_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX5767663.1"
                     /translation="MSDPVLALTMLGLFILLIMLGFPIAFTLMGMGVAFGYYAYYQPD
                     QAFFDSRLFYLLTQNTWSIMSNDVLVAVPLFLFMGYVVERANILDRLFFSLQVALRHI
                     PGSMAVAALVTCALFATATGIVGAVVTLMGLLAFPAMLKAGYDQRLSAGVICAGGTLG
                     ILIPPSIMLIVYAATASVSVVRLYAAALLPGLVLAGLYVVYVMGRAALNPKLAPPLPK
                     GEAAVPWVKALLLLAQAFVPLAALIFAVLGSILFGLATPTEAAAVGALGALVLAAAYR
                     ALTWSRLREAVYLTARTSAMVCFLFVGSWTFSSVFSYLGGEGLIKDFVLGLDLNPVAF
                     LLLSQLIIFVLGWPLEWSEIIIIFVPIFLPLLPHFGVDPMLFGILIALNLQTSFLTPP
                     MAMSAYYLKGIAPPHVQLWTIFQGCFPFVAMVFMTMLLVYVFPKLMFWLPELIYG"
     gene            complement(7467..8030)
                     /locus_tag="VFX94_05460"
     CDS             complement(7467..8030)
                     /locus_tag="VFX94_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX5767664.1"
                     /translation="MLERLVYAIDELSRMVGHAFAWCIIILTFGTSYEVFVRYALNDP
                     TSWAFDMSYLMYGALFFMAGAYTLSRNGHVRADFLFRRWRPRRQAAVELTLYVLFFFP
                     GVLALVLAGWGYGTDAFRIGEVSVNSPVGIPVWQLKLLIPAGAVLLTLQGLAESLRCA
                     ICLRDGDWPPRLHDVEETEELLRQARR"
     gene            complement(8046..9167)
                     /locus_tag="VFX94_05465"
     CDS             complement(8046..9167)
                     /locus_tag="VFX94_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX5767665.1"
                     /translation="MDNPTPRRKFLRSAAFGAAGGATLGFPAIARSQSTTVIKMQGSW
                     GATDIFNEMAMEFVKRVNDMSGGRLRVDYLVGGAVVKPFEVMDAVSKGVLDAGHTVPV
                     YWYGKSKVASLFGSGPINGCDAFQTLAWIYRGGGLKLYQELLDKLGLNVVGFFAMPMP
                     TQPLGWFRKPITDAGQLKGLKYRTVGLAADLFQSMGAKVTQLPGGEIIPALERGVIDA
                     FEFNNPTSDMRFGAQDVIKNYEMGSFHQAMEFFEIIINKKKWESLPKDLQAIWQYGVE
                     AASQSNYWTAMDNYSRDFHKLQKEHGVKVTRTPKSVFEAQLKAWDGITARLSKEDAFF
                     KKVVQSQQDWAKRVVPYWHTNDAPFAMGYEHVFKTKLPT"
     gene            9346..9888
                     /locus_tag="VFX94_05470"
     CDS             9346..9888
                     /locus_tag="VFX94_05470"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX5767666.1"
                     /translation="MATATSLGESLVLVARPQVQDPLYARTVLVVAPFGRDQHYGFIL
                     NRPTRYKLGEMFPGHAPSQKVAHPVFLGGPVDVRIIFALVPQAESPGPGSMELMPGLY
                     ATFHAQTVDRIIESKPDRARFVAGLVVWRPSELARELAGRMWYVLDADASLATREPAG
                     LWEELVLRAQRYGRMFPTSK"
     gene            complement(9903..10463)
                     /locus_tag="VFX94_05475"
     CDS             complement(9903..10463)
                     /locus_tag="VFX94_05475"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5767667.1"
                     /translation="MVAFAALLAATPLRADRIYWNDELASALIGKGVVNRRGQRLGEI
                     ADIVVDLRRGEARFALVESGGVLGFLRAQRAFPFAWLAPAKRENAILLDVASSELDAA
                     SRAPPPGLRATTLIGTTVYDSTGREAGELRDLVVNLGDGKLRHAVIALKRPERQVTIE
                     PEMLGVSAGSVVVNMRYDDLRALVAP"
     gene            10599..11018
                     /locus_tag="VFX94_05480"
     CDS             10599..11018
                     /locus_tag="VFX94_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767668.1"
                     /translation="MLKVLSARLHGVLDYVIALAFIVAPAALELAYPAEALAYITGSI
                     YLGVVLFSRYPLGMLELIPYPIHGLIEAVLAACWIGMPWLFEFAHHALARNFYVGAGA
                     ALLVVVLLTDYRSTRAGVWHHAERRHSLVDRRHYAAA"
     gene            complement(11226..>11616)
                     /locus_tag="VFX94_05485"
     CDS             complement(11226..>11616)
                     /locus_tag="VFX94_05485"
                     /inference="COORDINATES: protein motif:HMM:NF044606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767669.1"
                     /translation="LARTVLIGIAAYAALIAILRVSGKRTLSKMNAFDFVVTVALGST
                     LATILVDTSVTLAQGALALALLVALQFAITWTSVRVAWVRRIVTGEPALLARHGRCLP
                     DYRTRCSAHASPRRKCWPRCEALASPS"
BASE COUNT         2066 a         3854 c         3873 g         1823 t
ORIGIN      
        1 gtggtgattt tcatcttcat cgcaatctcc ttgaccgatc tcctatggaa tgggccttgc
       61 ggcacccctc gcataggagg tatgtgatgt tgaataccgc aagaagcgtg cttttcgtgt
      121 cctgcgccgt gctcgctgta acagcggcag cggaaggccc gcgcatcgat tcgcagacga
      181 gcggcgcgac gcgcggccag cccgccggcg gcgcgaccgg cgaaaccgcg ggcgcgatgc
      241 agggccccat ggcacccgac cgcaacgcgg tcgaggtcgc gccggcaggc atgcagccgt
      301 atctcaccga cgttcgcggc cgcgcgctct atatgctcga gggcgagaac gcgggcaagg
      361 tgcgctgcga cgcggcgtgc gcgcgagtgt ggccgccgct gcgcgacgag gcgaatctcg
      421 ccggcaaggg cctcaagaag acgctagtcg gcagccgcaa gcgtgccgac ggctcgagcc
      481 aggtcaccta taacggccac cccctctact actacgtcga agaccgcggc gccgggaaag
      541 cgaccgggca ggtgcacgat cgctggggcg agtggtacct cgtcacgccc cagggcggga
      601 aattcgagaa gcgctcttag cgcggttcgg gcgagcgctc gagctcggcg cgcgtgcgcc
      661 gcacggcgac ccgccttgca tgccagtcga cgctttcgac ggcggagggc ggcacgagga
      721 ctttcttccc cggcagccag ttgcgcgtat cgaccaccat gcccgtgatg gtccaggtgt
      781 cgctctcgac cacgaagtct tcgacatgtc cgagatcgcc gtcgcgcgcg tggatgtgat
      841 agcccacgag ctccgcggcg ctgcgcaggt gcgattgctc ggcgcgcttc tgcgcctgca
      901 ccgcggcctc gttgcgcgca tcgcccgggt cacgctcgag ctgcggcggc ggcacggcga
      961 gcggagaggg cgtgccgacc cagaggtacg gggcgctgcg gtacgcggga taggctgagt
     1021 ccgcgctgcg cgccgcttcg tgcaggcgcg atgccggcgg gtgctcggcc gggcccggcg
     1081 cgcgctcgag ctcttcgcgc gtaagctcga cctgcagctc gcgcgcgcga ccctcatgcg
     1141 ccctcaccag gtgcggagaa atgagcgcct cgcgaccgga aaaccagccg ccggtgtcca
     1201 ccacgaagta gcacaccgtc cagcgctcgt cgtcgaagta cacatccttc agtgagccga
     1261 gcttgccgtt acgcgccagg atggcgtcgc ccttcaggtc ctgcatcatc ttctgcttca
     1321 tcagccctcc tacgttaggt cgacatagaa cgcgaacgcg atcgcgagca cgctgttcag
     1381 cgcgagggcg acctggccgg gcccatggcg cagccgcgtc catccgtggc gcccgaggta
     1441 aagcacggcg agcccggctc cgacgagcgc gagagcgccc gcgagcagag cgagaccccg
     1501 cgtccagccc ggcagcagct cggcgttgaa gtgaatggcg agcagcccca gcgccagagc
     1561 cgcgcacaag tacgacgcgc gcacgagcag cttgccctcc ggcattgtca cccgaccttc
     1621 tgtagcgcga gccaggcggc gagcgcggcg cctacggtgt ctgccaggag atcggtcatg
     1681 gtgtcggtct tgccgcggat ggcgttgccg taagtgagcg cgtcgaagag ccattccgcg
     1741 atctcccatg cggtgccgag cgcgacaccg agcgcaatga ggatcgccac atgcgccagg
     1801 gcccgctgcg cgcccggcaa cgcgccgcgc atatgcgcgg cgaggagaag cgtgagcgca
     1861 aagatcgtaa gcgcatgcaa tgcgagatca aaacccggaa tcgcataaag cgaagcagcg
     1921 taggccgcgg cattcagcgc cgcggccagc gccgcgcttc cccaggccag agggcgcagt
     1981 ccgctggaag atgtacagcg ggagcgcaag ctccgtcagc gagtcttgcc actcgccgag
     2041 ggtgcaatgc gcaagtcgtt ctgcaccgag cgcaccccct cgacgtcctt tgccgcattc
     2101 tcggcgcggc gcttcgcttc gtcggactcg acaaagcccg acagctgcac caccccgcga
     2161 tacgtcgtga cattcaccga ggccgcgttc accttgtctt cctcgacgag ttgctgcttc
     2221 accttcgcgg tgagcgcggc atcgtcaatc acctggccgg tcgagcgatc ctgtgctgtg
     2281 gcgctgcagc caatgagcgc tgcagcgaag aaataggcga tgattctgac ttaataggca
     2341 tagcgcgaga ctccttggtt tgaaaagaac gaacacgcct catcgcgcaa gtcatgtgcc
     2401 cgctccgcac ttgctgtcta tagctcgggc cgctacagcg cttccgacat ttagctacct
     2461 gcgcttaagc cacggataat gcgccgccgg ttagtctggc tacatgcgaa ttcaaccaag
     2521 tccattcaga gtacagaagt tccttgccgg gctgcgctat cccgcgcgca agcagcaggt
     2581 cctcgagcgg gcgcgcagcc gcggcgcgga tgcgcagctg atcaggctgc tcttgctctt
     2641 gcccgagcgc gattacgaaa gcccgatctc gctctcgtgc gaagtgggac gtcaggccga
     2701 gcgctcgcgc aattgatccg ctcgatgcgc aggctcacgc tcgccgccgc gttgtcggcg
     2761 ctcgcgctgt cactccctgc agaggcgggg gccgtggcgg cgggcgttct gatcggccgc
     2821 ccgatccagg atttgagtgg ccaggtgctc ggcacggtcg aggacatgat cgtcgacgtg
     2881 cgcgacgctc gcgtgctgta cataatcgtc gatgcgcgcg agcgcgattt cacgctgccg
     2941 atccgcgcat tgcgcgagca cgaggggggc gtgcgcctcg acatgagcga cgcgggcgag
     3001 atcgcgcgca ccgacgaccc gcgctttcgc cgcgccgcgc gcctcatcgg ccagccgctc
     3061 atgaatccca acggcgggcg catcggcacc atcgccgaca tcgagttcga tcccgccgcc
     3121 ggtcgggtcg agcgcgtcgt cgtcgacgtg gacaatggca aggccaactt tccgccgagc
     3181 gtgctcgcac acggccgctt cccgccgctc acgcggtggc aggcggagca tccgccgagc
     3241 gacttctccg gcgatccggg ctacgtgcgc cgcgacccgt cgcccgagcg cgagcgcctg
     3301 cacgatcacc gctggccgcg caattagcgg caggtattgt ccgccgtcac ctcgcgcagc
     3361 ttctcgccaa gcggcttgcc ttcggtccag gtaccgagca ccgcctcgac gagcgcatgc
     3421 ccgctcgagg aagctatcaa gcggtcgttg attacgagac gcacgccggc gtccggcagg
     3481 taggcgatga agagctcatc gcccgggcgg agacgccggt aggctgcgcg ggcgcgcgcc
     3541 agatcgtcca ccgagaggtg gcgcacgatc ggctcgcgcc actcgcgcgg gatgcgcaat
     3601 ggcagatgat cgtcattgag aatgcgcacg tagagcgcct tggcgctcgt cgggtcgagg
     3661 agctcctcga gcgcgctcct gcgctcgggc aggtacagcg cagcgacgta gtgctcgatc
     3721 cagagcgtgc tgcgcacgcc gcagatcgcc gcctccagcc tgatttcgcc gaagcgcagc
     3781 gcccccggaa cgctctcagc gccggccgcg ccggcgataa caaacgcggc ggcggcgagc
     3841 gctctcacgc gccgctagtc cgccgccagc gcgagcgcga tgttgttgaa ctcgagcggc
     3901 gtgaggcggc cgttgcggtc gaggtcggcg gggccgaaat tcgcctcgac cggcgggctt
     3961 gccgccgctt cctcgcgcga aagcgagaaa tcgtagttgc tatcgagcgc cgcaaacatc
     4021 gcgcgcggaa tgacgcgcgg cgcttcggcc cacggccgtt gctcggccca ttgggggtcg
     4081 ggcggccagt cgcgccactc cgtcacgggg tcggccgcga gcaaggcgga agcagcggtc
     4141 gcgagcagcg cactgcagac gcagacgatg cgcatcgcct attcggtccc ttcttcgtcg
     4201 cccatagtgt cgaggacgtc gcgcacgtcg tttaccatga cgagcgcctc gtcggcgata
     4261 tcggccacgg ctttttgctc cgcggccgca tcggccagga gctcggcgag cgcatccagg
     4321 ttcttgcgag cgctcaggag gtaatcgacc gctgccattg cgggcgtcct cgcaatggcc
     4381 gtgccacgcc cacgcaggca cacatcgtgc gccgggatgg cggctatgcg ttacgcagtg
     4441 atcgaccccg ccgccacgat cgaaagcgca agtgctgcat tgcgtgcctg cgatgcggac
     4501 gcgctgctcg tgatcggtgc gcggccacag cccgccagcc cggtactgtc ggcacgcgac
     4561 atcgtcaccc gcgtggtagc cgtggggctc gacccccgcg tggtgaccgt gagcgatctt
     4621 ctgcgcgtca tggcggaccg gccgctgtaa ttcttcgccg gcgctcgtaa cggcttacag
     4681 cggaagccgc gcgcggcgag ctcgtctaat gtcggacgct cacattgtct taagtcggtc
     4741 catgcactac agcgtgctcg gtttcacagc gctgagcgcc aggcgcgcga cctaaaaaat
     4801 gaaatcgaac acgccaatta cgacgagcag cccgatgagg aagatgatga ggacaatcca
     4861 acccaagacc ttgaccatgc actgatcctt cgcggttagc gagcgggaat gcgctccgtg
     4921 atcaccttga cgcgcagcac gggctcgccg tccacctgac gcgcctccgc cgtgagcacc
     4981 acctcgtcgc ctttctggat cgtgccctcg ccgccgggcg cgcccagatc cagcacgaag
     5041 gagccgccat cggccgtatg caggcgcgcg aactgctgag cgccttggct tgtttgcgcg
     5101 acccagcggg ccgtgatcgt tccgctgaac tgcctgattt cgtcgccgag cacctcgtgc
     5161 ggtgccatcg cgagcgtgac caacagcagc aagagaaatt ttcgaatgcg gcccatcccc
     5221 ccggggccgc aatcgtcgtg ccgtacttgt cacagagatt gttcccgatc acggccgatg
     5281 aaataggtgg cgagggccgc agtgacgtag ccgaaaatcg cgaagccgta gatggcgagc
     5341 gcgaggcaga gcgcgcggcc gtcgggcgtc tttggccagt actccgagcc catcgtgctc
     5401 acgagcattg ccgtccacca cagcgccgtg gcgaagctgt cgaatccgcc gtccggcgcg
     5461 tcgcgctcga acgcgtacat gccggccgcg cctgcgaaca ccaccaccat ggtgagcagc
     5521 agcacgtagc cgaagccgcg tcgctgcatc gccgcgccga gcgcagccat ggcgcggttc
     5581 accgaggcga ctacccggag gagccgcagc ccgcggctcg cgcgtgcgaa gcgtgcaagg
     5641 cgcgcgatgc gcagcacgcg caacgcaggc gcggccagtg ccagcacctt gagccagttt
     5701 tcccgtaggt agcgcacgcg ctccggagcg agcgcaagcg agagcgcaaa ctcggcgatg
     5761 aacagcaccc agatggcctg gccgagtatc cagagcgcgc ggttcaatcc ccaggcgagc
     5821 tccacgacga ggagcacgag ccagacaaat gagagcgcga tcatcggttt ttccagccac
     5881 tcctgcagct cgcgcaagat ctgcgcgcgg tccggctcct ccatggctcc tccgattggg
     5941 atggacaccc gcatgttaat cgcgcgcgtc acgagcgcag cgattgtgcg agatcgatga
     6001 gcatgagtac gaagcccacc gccagtacgg ccagaagcgc atagtcgtcg acgagcttca
     6061 gggccacggg cgcgatgaag ccggcgagca ggcacaccgc gagcgcggcg gcgaggaggc
     6121 ggccgatcat ccgtagatca gctcgggcag ccagaacatg agcttgggaa acacatagac
     6181 gagcagcatc gtcatgaaca ccatggcgac gaacggaaag cagccctgga agatggtcca
     6241 gagctgtacg tggggcgggg cgatgccctt caggtagtac gcggacatcg ccatcggcgg
     6301 ggtgaggaac gaggtctgca ggttgagcgc gatcaggatg ccgaagagca ttggatccac
     6361 gccgaaatgc ggcaacagcg gcaggaagat cggcacgaag atgatgatga tctcgctcca
     6421 ctcgagcggc cagccgagaa cgaaaatgat cagctgtgaa agcagcagga acgccaccgg
     6481 gttgaggtcg agcccgagca cgaagtcctt gatcagcccc tccccgccga ggtacgagaa
     6541 caccgacgag aaagtccatg agccgacgaa cagaaagcaa accatcgcgg aggtgcgcgc
     6601 ggtgagatag acggcctcgc gcaggcggct ccaggtgagc gcgcggtacg cggcggccag
     6661 caccagcgcg ccgagcgcgc cgaccgcggc agcctcggtc ggtgtggcga ggccgaagag
     6721 gatcgagccg agcacggcga agatcagcgc cgcgagcggc acgaatgcct gcgcgagcag
     6781 gagcaacgct ttgacccacg gcaccgcggc ttcgcctttc ggcagcggcg gggcgagctt
     6841 gggattgagc gccgcccgcc ccatgacgta gaccacgtac aggccggcga gcaccaggcc
     6901 cggcagcaaa gcggcggcgt agagccgcac caccgacacc gaggccgtgg cggcgtagac
     6961 gatgagcatg atggacggcg ggatcaggat gccgagcgtg ccgccggcac agatcacgcc
     7021 ggcggagagg cgctggtcgt agcccgcttt gagcatggcg ggaaaggcga gcagtcccat
     7081 tagcgtgacc accgcgccca cgatgccggt ggcggtggcg aagagcgcac aggtgacgag
     7141 ggccgccacc gccatcgacc cgggaatatg gcgcagcgcg acttgcaggc tgaagaacag
     7201 gcgatcgagg atgttggcgc gctcgaccac gtagcccatg aagaggaaga gcggcaccgc
     7261 cacgagcacg tcgttgctca tgatgctcca cgtattctgc gtgagcagat agaacagacg
     7321 gctgtcgaag aacgcctgat cgggctggta gtaagcgtag tagccgaatg cgacgcccat
     7381 gcccatcagc gtgaacgcga taggaaagcc gagcatgatg agcaggatga agagcccgag
     7441 catcgtgagc gcgaggaccg gatcgctcat cggcgcgcct ggcgcaggag ttcctcggtc
     7501 tcctcgacat cgtgcaggcg cggcggccaa tctccgtcgc gcaggcatat tgcgcagcgc
     7561 agggactcgg cgagcccctg cagcgtgagc agcaccgcgc cggctggaat gagcagtttg
     7621 agctgccaga cggggatgcc gaccgggctg ttgacgctca cctcgccgat gcgaaacgcg
     7681 tcagtgccgt agccccagcc ggccagcacg agagcgagca cgccgggaaa aaagaacagc
     7741 acgtagagcg tgagctctac cgcggcctgg cgccgcggcc gccagcgccg gaacagaaag
     7801 tcggcgcgca cgtggccgtt gcgcgacagc gtataggcgc cggccatgaa gaaaagcgcc
     7861 ccgtacatga ggtagctcat gtcgaaggcc cagctcgtcg ggtcgttcag cgcgtagcgc
     7921 acgaacacct cgtaggaggt gccgaaggtc aggatgatga tgcaccacgc gaacgcgtgg
     7981 cccaccatcc ggctcagctc gtcgatcgcg tagacgagcc gctcaagcac cgcggccgcc
     8041 cgcgctcagg tcggcagctt ggtcttgaac acgtgctcgt agcccatggc gaagggggcg
     8101 tcgttcgtgt gccagtacgg caccacgcgc tttgcccaat cctgctgcga ctgcaccacc
     8161 ttcttgaaga acgcgtcttc cttggacagc cgcgcggtaa tcccgtccca ggccttgagc
     8221 tgggcctcga acaccgactt cggcgtgcgc gtcaccttca cgccgtgctc cttctgcagc
     8281 ttgtggaagt cacgtgagta gttgtccatc gcagtccaat agttcgactg cgaagccgcc
     8341 tcgacaccgt actgccagat cgcctgcagg tccttcggca gcgactccca cttcttctta
     8401 ttgatgatga tttcgaaaaa ttccatcgcc tggtggaagc tgcccatctc gtagttcttg
     8461 atcacgtcct gcgcgccgaa gcgcatgtcg gaggtcggat tgttgaactc gaaggcgtcg
     8521 atcacgccgc gctcgagcgc cgggatgatc tcgccgccgg gaagctgcgt cactttcgcc
     8581 cccatgctct gaaaaaggtc cgccgcgagt cccacggtgc ggtacttgag gcccttcagc
     8641 tgacctgcat cggtgatcgg cttgcggaac cagccgagcg gctgcgtcgg catgggcatc
     8701 gcgaagaagc ccaccacgtt caagccgagc ttgtcgagga gctcctgata gagcttgagg
     8761 ccgccgccgc gatagatcca ggcgagcgtc tggaacgcgt cgcagccgtt gatcggcccg
     8821 gagccgaaga gcgaagcgac cttcgacttg ccgtaccagt acaccggcac cgtatggccg
     8881 gcgtcgagca cgcccttgct caccgcatcc atcacctcga agggcttcac caccgcgccg
     8941 ccgacgagat agtcaacacg cagcctgccg cccgacatgt cattcacgcg tttcacgaac
     9001 tccatcgcca tctcattgaa gatgtcggtc gcgccccagg agccctgcat cttgatgacc
     9061 gtggtcgact gcgagcgcgc gatggcggga aaaccgagcg tcgcgccgcc ggcagctccg
     9121 aatgccgcgc tgcgcagaaa ttttcggcgg ggtgtgggat tgtccatggt gtgctcctga
     9181 ccgggggaaa gggaggggag gggcgtacct gcgcagcgtg cgcgcgcgac gcttaagccg
     9241 cgcttaagcg tcccttaatt gcgccgctgc aatcgttgcg gtgtgccgaa catgcgcaaa
     9301 ctcctagtcg ccttgtttct cgctctcgcg gctgggatgc cgctgttggc gacggcgaca
     9361 tcgcttgggg agtcgctcgt cctggtcgca cggccgcagg tgcaagatcc gctctacgcg
     9421 cgcaccgtgc tagtagtggc gccgttcggc cgggatcagc attacggatt catcctcaac
     9481 cgcccgacgc gctacaagct cggcgagatg ttcccgggcc acgcgccgtc gcagaaggtg
     9541 gcgcaccccg tgtttctcgg ggggccggtc gacgtgcgga tcatcttcgc actcgtgccg
     9601 caggccgaaa gccccggtcc gggttcgatg gagctcatgc cggggctcta cgccaccttc
     9661 cacgcgcaga cggtcgatcg catcattgaa tcaaagccgg accgcgcgcg cttcgtcgcc
     9721 ggcttggtgg tgtggcggcc gagcgagctc gcgcgcgagc tcgccggacg gatgtggtat
     9781 gtgctcgacg ccgatgcctc gctcgccacg cgcgagccgg caggactgtg ggaagagctc
     9841 gtcctgcgcg ctcagcgcta cgggcgtatg tttccgacgt cgaagtagag cgtgatcggt
     9901 gcctacgggg cgacgagcgc tcgcagatcg tcgtagcgca tattgaccac gaccgagcca
     9961 gcgctgacgc ccagcatctc tggctcgatc gtcacttgcc tctcggggcg cttgagggcg
    10021 atcaccgcgt gacgcagctt gccgtcgccg aggttgacga ccaaatcgcg cagctcgccc
    10081 gcctcgcggc cggtcgaatc gtaaaccgtt gtgccgatga gggttgtcgc tcgcagcccg
    10141 ggtggtgggg cgcgactcgc ggcgtcgagc tcgctggagg ccacgtcgag caagatcgcg
    10201 ttttcgcgtt tggcgggcgc gagccaggcg aaggggaaag cgcgttgcgc ccggagaaat
    10261 cccagcacgc cacccgattc gaccagcgcg aatcgcgcct cgccgcgccg gaggtcgacc
    10321 acgatgtccg caatctcgcc cagccgttgg ccgcggcggt tgacgacgcc cttgccgatg
    10381 agcgcgctcg caagctcgtc gttccagtag atgcgatcgg cgcgcagcgg cgtcgcggcg
    10441 agcagagcgg cgaacgcgac catcaaccag ctcgcgccac gagcgcaacg ccgtggcatc
    10501 gctgcaagtc ctacagcggt aagcgaagct ctgcgttcac ccatggccgg gggctcgcaa
    10561 gggccgtacc ggtcgcgcga ttttgctaag gacaaagcat gctcaaagtg ctcagcgcgc
    10621 gccttcacgg ggtgctcgac tatgtcatcg cgctcgcgtt catcgtggcg ccggcggcgc
    10681 tcgagctcgc ctatccggcc gaggccctcg cctacatcac cggcagcatc tatctcgggg
    10741 tggtgctctt ctcccgctat ccgctcggca tgcttgagct cattccgtat ccgatccatg
    10801 gcctgatcga ggccgtgctc gctgcctgct ggatcggcat gccgtggctg ttcgagttcg
    10861 cccatcatgc gcttgcgcgc aacttctacg tcggcgctgg cgcggcgctc ctcgtcgtcg
    10921 tgctgctcac ggactaccgc tcaacgcgcg ccggcgtctg gcatcacgcg gagcgccgtc
    10981 actcgctcgt cgaccggcgc cactacgctg cggcctgagc ggcgctcccg ggctctaggc
    11041 ggcgagggcc ggcgcgcccg cgcatggaga acacgggcta cgccggccat tcgcgcggac
    11101 cagcaaggaa gagtgaccgc gcgtcgccag gaccatgttg caacatagga acttaaacgg
    11161 tgcttaattc gtgcgaatga cgctgaacga gccgtcggtt tcaagcacca cggcttcggc
    11221 tttgctcagc tcggcgaggc cagcgcctcg caccgcggcc agcacttcct cctcggtgac
    11281 gcgtgcgcgg agcaacgcgt ccggtagtcc ggtaaacagc ggccgtggcg ggcaagaagg
    11341 gcaggctccc ctgtgacgat gcggcggacc caggccacgc gcacgctggt ccaggtgatc
    11401 gcgaactgga gcgcgacgag caaggcgagc gcgagagcgc cctgcgcgag cgtcacgctc
    11461 gtatcgacga ggatggtggc gagggtagaa ccgagcgcga cggtgacgac gaagtcaaag
    11521 gcattcatct tggacagcgt gcgcttgccg gatacgcgca ggatcgcgat cagcgcggcg
    11581 taggcagcga taccgatgag gacggtgcgg gcgagc
//
LOCUS       DASUNT010000272         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_300698, whole genome shotgun sequence.
ACCESSION   DASUNT010000272 DASUNT010000000
VERSION     DASUNT010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300698"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFX94_05490"
     CDS             <1..322
                     /locus_tag="VFX94_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5767670.1"
                     /translation="YLHSLERLLAEDIAIIAPGHGYLIGAPHKEVRRLIAHRLNRERK
                     VVAALERLEHPSLEEMLPLVYDDVPERIHRVAARSLTAHLDKLVAEGAVRLEESRYTL
                     VRSA"
     gene            336..605
                     /locus_tag="VFX94_05495"
     CDS             336..605
                     /locus_tag="VFX94_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767671.1"
                     /translation="MTSESEKTMKLVTNLDRQAIEGKLAEVRSAAQAANLKELADMFN
                     GVEGMPRAQIEQKVKNALKWLADKPQHNRIASQLELIGLNLPNLK"
     gene            607..1953
                     /gene="gor"
                     /locus_tag="VFX94_05500"
     CDS             607..1953
                     /gene="gor"
                     /locus_tag="VFX94_05500"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEX5767672.1"
                     /translation="MDFDYDLFTIGGGSGGVRASRVSAGYGAKVAIAEEGRFGGTCVN
                     VGCIPKKLFSYAAHFREDFEIARAYGWTVGEPRFDWPTLLGNKDREIARLNGVYERVL
                     VNAGVEIHRARAHVLDPHTVELGGRKFTAKHILVATGSWPVLPALPGIEHAITSNEAF
                     ALELLPRRAIVVGAGYIALEFASIFHGLGVQTTLTYRGKRLLRGFDAELGTRMAEEMA
                     AKGMTICFGCEPAAIRKKQSLEIEFTDGSLRETDLVLFATGRRPNTAKLGLEHAGVQL
                     AADGAVIVDRYSASSVPSIHAIGDVTNRINLTPVATAEAMWLARTLFRNQPTPIDHDN
                     VPTAVFANPNLATVGLSEEKARERYGAVDIYKASFRALKLTMTEKTERTFMKLVVERS
                     SQRVVGAHMIGADAGEVIQGVAIAVKLGATKQQFDQTIGIHPTAAEEFVTLREPSK"
BASE COUNT          364 a          692 c          680 g          294 t
ORIGIN      
        1 ctatctgcac tcgctcgagc ggctgctggc ggaggacatc gccatcatcg cgccgggaca
       61 cggctacctg atcggcgcgc cgcacaagga agtgcgccgc ctgatcgcgc accgcctcaa
      121 ccgcgagcgc aaggtcgtcg cggcgctcga gcgcctcgag catccgtcgc tcgaggagat
      181 gctgccgctg gtctacgacg acgtgcccga gcgcatccac cgcgtggccg cgcgctcgct
      241 caccgcgcac ctcgacaagc tggtggccga aggcgccgtg cggctcgagg agagccgcta
      301 tacgctggta agatcggcat gatgggaggc acgcgatgac cagtgaatcc gaaaagacca
      361 tgaagctggt gaccaacctc gaccgccagg cgatcgaggg caagctcgcc gaggtgcgct
      421 cggccgcgca ggccgccaac ctgaaagagc tggccgacat gttcaacggc gtcgaaggca
      481 tgccgcgcgc gcagatcgag cagaaggtga agaacgcgct caagtggctc gccgacaagc
      541 cgcagcacaa ccgcattgcc tcgcagctcg agttgatcgg gctcaatctg ccgaacctga
      601 agtagcctgg atttcgatta cgaccttttc acgatcggcg gcggctcggg cggcgtgcgc
      661 gccagccgcg tgtccgccgg ctacggcgcc aaggtagcga tcgccgagga aggccgcttc
      721 ggcggcacct gcgtcaacgt cggctgcatc ccgaagaagc tgttttccta cgccgcgcat
      781 ttccgcgagg atttcgagat cgccagggcg tacggctgga cggtgggcga gccgcgcttc
      841 gactggccga cgctccttgg caacaaggac cgcgagatcg cgcgcctcaa cggcgtctac
      901 gagcgcgtgc tggtcaatgc gggcgtggag atccaccggg cccgggcgca cgtcctcgat
      961 ccgcacacgg tggagctggg cgggcgcaag ttcacggcga agcacattct ggtcgccacc
     1021 ggctcctggc cggtgctccc cgcgctgccg gggatcgagc atgcgattac ttcgaatgag
     1081 gcgttcgcgc tcgagctgct gccgcggcgc gcgatcgtgg tcggcgccgg ctacatcgcg
     1141 ctcgagttcg cctcgatctt ccacggcctg ggggtgcaga ccacgctcac ctaccgcggc
     1201 aagcggctgc tgcgcggctt cgacgcggag ctcggcacgc gcatggcgga ggaaatggcg
     1261 gcgaagggca tgaccatctg cttcggctgc gaaccggcgg cgatccgaaa aaagcagagc
     1321 ctcgaaatcg agttcaccga cggcagtctg cgcgagaccg acctggtcct gttcgccacc
     1381 ggccgaaggc cgaacaccgc aaagctgggc ctcgagcacg cgggcgtgca gctcgccgcc
     1441 gacggcgccg tgatcgtcga ccgctactcg gcgagcagcg tgccttcgat ccacgcgatc
     1501 ggcgacgtga ccaaccgcat caacctcacc ccggtcgcca ccgccgaggc gatgtggctc
     1561 gcgcgcacgc tgttccgcaa ccagccgacg cccatcgacc acgacaacgt gccgaccgcg
     1621 gtgttcgcca accccaacct cgctaccgtc ggcctgtcgg aggagaaggc gcgcgagcgc
     1681 tatggcgcgg tcgacatcta caaggcgtcg ttccgcgcgc tcaagctcac catgacggag
     1741 aagaccgagc gcaccttcat gaagctggtg gtcgagcgct cgagccagcg cgtggtcggc
     1801 gcgcacatga tcggcgccga cgccggcgag gtgatccagg gcgtggccat cgcggtgaag
     1861 ctcggcgcca ccaagcagca gttcgaccag acgatcggga tccaccccac ggcggcggaa
     1921 gaattcgtga cgctgcggga gccgagcaag taggaccgtt tcttgctcgg cgatgtccac
     1981 tatggcacgc caccaatcgg tctggtccgg caccgcagca atgccggagt
//
LOCUS       DASUNT010000273         3396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_826496, whole genome shotgun sequence.
ACCESSION   DASUNT010000273 DASUNT010000000
VERSION     DASUNT010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826496"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1881
                     /locus_tag="VFX94_05505"
     CDS             <1..1881
                     /locus_tag="VFX94_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5767673.1"
                     /translation="DQGGECQLQDLAVGYGGSNSRYREPKRVVTDKNLGPLIATDMTR
                     CIHCTRCVRFGQEIAGVMELGLAGRGENAEIVSFVGRAVESELSGNMIDLCPVGALTS
                     EPFRYKARTWELTRRRSIAPHDSLGSNLVVQIKQNKVMRVLPLENDAINECWLSDKDR
                     FSYDGLNSPDRLQSPMLKRDGKWEEVDWEQAFDFLLPRLQDSDFGVLASPHSTLEELF
                     LVSRLGAPADFRLRQSDFSADGKRAGIPWLGIPVAELEKLDRVLVIGSFLRKDHPLIA
                     QRLRQAAKRGAQIHMLHSVDDDWLMPIASKKIVRPSEIAADTGSFKDLLTGGKAAAVF
                     LGNFALQHPQAAQIHAAAQALGVTVGIFGEAANSVGGYLAGLPAGGDVNEVLKKKSFV
                     LLNVEPELDCADPLAARRAIHAADFVVSLSPWKTGLDYADVLLPIAPFTETAGTFVNT
                     EGRVQSFSAVVNPLGASRPGWKVLRVLGSLLGRQGFDFDTVEQVRAACLGGRDVQGLL
                     SNAISVEMRNSDKPRGIQRIADVPMYFADPLVRRSPPLQRTRDARPPRAWMNSRLLAR
                     LGVAAGQPVLVNGAAKLLAALDDKLPEDCARIAAAHPATAAVGPMFGALEIARVAGEK
                     AA"
     gene            1882..2958
                     /gene="nuoH"
                     /locus_tag="VFX94_05510"
     CDS             1882..2958
                     /gene="nuoH"
                     /locus_tag="VFX94_05510"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX5767674.1"
                     /translation="MEWFADRSNAWFGPVWGPAFYETATSLVFIVAILVPLILSVAYL
                     TLWERKLIGWMQVRLGPNRVGPLGLLQPFADVLKLLFKEIILPTNASKALYLLAPVVM
                     IMPAIAAWAVIPFSPVLVLSDINAGLLYIMALSSFGVYGVIIAGWASNSKYAFLGAMR
                     SAAQMVSYELAMGFALVVVLMVSASLNLSDIVEAQGKGYFASKGITFLSWNWLPLAPM
                     LLVYFVSGVAETNRAPFDVVEGESEIVAGHMIEYSGMTFALFFLAEYMNMLLISALTA
                     IMFFGGWQPPVDSIVFNWIPPFGWLLIKIFFIVSMFLWIRATFPRYRYDQIMRLGWKV
                     FIPLTLVWIAVVGIWMQTPWNIWK"
     gene            2966..>3396
                     /gene="nuoI"
                     /locus_tag="VFX94_05515"
     CDS             2966..>3396
                     /gene="nuoI"
                     /locus_tag="VFX94_05515"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX5767675.1"
                     /translation="MTRVLDFFRSFLLLELFRGMMLTGKYVFARKITIQFPEEKTPQS
                     PRFRGLHALRRYPNGEERCIACKLCEAVCPALAITIEAAPRADDGSRRTTRYEIDLFK
                     CIYCGFCEESCPVDSIVETRILEYHGEKRGDLIYTKEMLLAV"
BASE COUNT          533 a         1130 c         1121 g          612 t
ORIGIN      
        1 gaccagggcg gcgagtgcca gctgcaggat ctggccgtgg ggtatggcgg cagcaactcg
       61 cgctatcgcg agcccaagcg cgtggtcacg gacaagaacc tcgggccgct catcgcgacc
      121 gacatgacgc gctgcatcca ctgcacccgc tgcgtgcgct tcgggcagga gatcgccggc
      181 gtcatggagc tcggcctcgc cgggcgtggc gagaacgccg agatcgtgtc gttcgtcggc
      241 cgcgcggtcg agtccgagct ttccggcaac atgatcgacc tctgcccggt gggcgcgctg
      301 accagcgagc cgttccgcta caaggcgcgc acctgggagc tcacgcgccg gcgctcgatc
      361 gcgccgcacg attcgctcgg ctcgaacctc gtggtgcaga taaagcagaa caaggtgatg
      421 cgcgtcctgc cgctcgagaa cgacgccatc aacgagtgct ggctctcgga caaggaccgc
      481 ttctcctacg acggccttaa ctcccccgat cgcctgcaaa gcccgatgct gaagcgcgac
      541 ggcaagtggg aggaggtgga ctgggagcag gcgttcgact tcctgctgcc gcggctgcag
      601 gacagcgatt tcggcgtgct cgcctcgccg cattcgacgc tggaggagct tttcctcgtc
      661 tccaggctcg gcgcgccggc cgatttccgg ctgcgccaaa gcgacttctc cgccgatggc
      721 aagcgcgccg gcatcccgtg gctcggcatc ccggtcgccg agctcgaaaa gctcgaccgg
      781 gtgctcgtca tcggctcgtt cctgcgcaag gaccatccgc tcatcgccca gcggctgcgc
      841 caggctgcga agcgcggcgc acagattcac atgctgcact cggtggacga cgactggctg
      901 atgccgatcg cgagcaagaa gatcgtgcgg ccgtcggaaa tcgccgctga caccggatcg
      961 ttcaaggatt tgctcaccgg gggcaaagcg gcggcggtgt tcctcgggaa cttcgctctg
     1021 cagcacccgc aggcggccca gatacacgcg gctgcccagg cgctcggggt taccgtaggg
     1081 atcttcgggg aggcggccaa ctcggtcggc ggctacctcg ccggcctgcc ggcggggggc
     1141 gacgtcaacg aagtcctgaa gaagaagagc ttcgtcctcc tgaatgtcga gcccgagctt
     1201 gactgcgccg atccgctcgc cgcgcggcga gccatccacg cggccgattt tgtcgtgagc
     1261 ctctcgccct ggaagacggg gctcgactac gccgacgtcc tgctaccgat cgcgcccttc
     1321 accgagaccg cgggcacgtt cgtgaacacc gagggtcggg tgcagagctt ctccgccgtc
     1381 gtgaacccgc tcggcgccag ccgcccgggc tggaaagtgc tgcgcgtcct ggggagcctc
     1441 ctcggccggc agggcttcga cttcgacacg gtggagcagg tgcgcgccgc ctgcctgggt
     1501 ggcagggatg tgcaaggcct gctgtcgaat gcgatttcgg tcgaaatgcg caattcggac
     1561 aaaccgcgcg gcattcagcg catcgccgac gtgccgatgt acttcgccga tccgctggtg
     1621 cgccgctcgc cgccgctcca gaggacgcgc gacgcgcggc cgccgcgcgc gtggatgaac
     1681 tcgaggctgc ttgcgaggct cggcgtcgcc gccgggcagc cggtgctggt gaacggcgcg
     1741 gcgaagctgc tggcggcgct cgacgacaag ctgcccgagg actgcgcgcg catcgcggcg
     1801 gcgcatccgg cgaccgcggc ggtgggaccg atgttcgggg cgctcgaaat cgcgcgggtg
     1861 gcgggggaga aagcggccta gatggaatgg ttcgccgaca gatcgaatgc ctggttcggc
     1921 cccgtctggg ggccggcctt ctacgagacc gcgacgagcc tcgtcttcat cgtcgcgatc
     1981 ctcgtgccgc tcatcctttc ggtcgcctac ctcacgttgt gggagagaaa gctcatcggc
     2041 tggatgcagg tccgcctcgg ccccaaccgc gtcggcccgc tcgggctgct gcagccgttc
     2101 gccgacgtgc tgaagctcct gttcaaggag atcatccttc cgaccaacgc gagcaaggcg
     2161 ctctacctgc tcgcgccggt ggtaatgatc atgccggcga tcgcggcctg ggcggtgatc
     2221 cccttctcgc cggtgctcgt cctctcggac atcaacgccg gcctgctcta catcatggcg
     2281 ctctcctcgt tcggggtata cggggtgatc atcgccggct gggcctccaa ctcgaagtac
     2341 gccttcctcg gcgcgatgcg ctcggccgcg cagatggtgt cctacgagct cgccatgggg
     2401 tttgcgctcg tcgtggtgct catggtgtcg gcaagcctga acctgtccga catcgtcgaa
     2461 gcgcaaggca agggctactt cgcgtccaag ggcatcacgt tcctctcctg gaactggctg
     2521 ccgcttgcgc ccatgctgct ggtctatttc gtgagcggcg tcgccgagac caaccgtgcg
     2581 ccgttcgacg tggtcgaagg cgagtccgag atcgtggccg gccacatgat cgagtactcc
     2641 gggatgacgt tcgcgctgtt cttcctcgcc gagtacatga acatgctgct gatcagcgcg
     2701 cttaccgcga tcatgttctt cgggggctgg cagccgccgg tggactcgat cgtattcaac
     2761 tggatcccgc cgttcggctg gctcctcatc aagatcttct tcatcgtgtc gatgttcctc
     2821 tggatccgcg ccacgttccc gcgctaccgc tacgaccaga tcatgcgcct gggctggaag
     2881 gtctttattc cgctcacgct cgtgtggatc gcggtggtcg ggatctggat gcagacgccg
     2941 tggaatatct ggaaatgagg caggcgtgac gcgcgttctc gacttcttca ggtcgttcct
     3001 gctcctcgag ctcttccgcg gcatgatgct cacgggcaag tacgtgttcg cgcgcaagat
     3061 cacgatccag ttcccggagg agaagacgcc gcagagcccg cgcttccgcg gccttcacgc
     3121 attgcgccgt taccccaatg gcgaagagcg ctgcatcgcc tgcaagctgt gcgaggcggt
     3181 atgcccggcg ctggcgatca ccatcgaggc cgcgccgcgc gccgacgacg gctcgcgccg
     3241 caccacccgc tacgagatcg acctgttcaa gtgcatctac tgcggcttct gcgaagagag
     3301 ctgcccggtc gattcgatcg tcgagacccg aatcctcgaa taccacggcg agaagcgggg
     3361 cgatctcatc tataccaagg aaatgctgct ggcggt
//
LOCUS       DASUNT010000274         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1001275, whole genome shotgun sequence.
ACCESSION   DASUNT010000274 DASUNT010000000
VERSION     DASUNT010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001275"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VFX94_05520"
     CDS             <1..278
                     /locus_tag="VFX94_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5767676.1"
                     /translation="ALTSGNPAKRFGLNSKGDIAPGLDADLALVDPKHTWTIHAKDSE
                     STQGYTPFEGIELSAKVKATFLRGSLICENGNVLGKPKGRYMHRPTP"
     gene            275..1018
                     /locus_tag="VFX94_05525"
     CDS             275..1018
                     /locus_tag="VFX94_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017424363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HEX5767677.1"
                     /translation="MNLELKGRSVLVTGGSKGIGLACAKALAAEGCRVHIASRNKETL
                     DRAAQAIGGETRVHAVDLRDGKALRALAEACADIDILVNNAGDIPGGTIEAIDEAKWR
                     HAWELKVFGYVNLTRELFARMKARKSGVIVNVIGMAGENPSFEYVCGSMANAGLGAFT
                     KAMGKGSSAFGVRVLGVHPPATRTDRIIPLMKAAAREKLGDENRWQELIGTGSFGQMI
                     EPEQVADIVTFLASARAGKLSGVMINLGA"
     gene            1023..1910
                     /locus_tag="VFX94_05530"
     CDS             1023..1910
                     /locus_tag="VFX94_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5767678.1"
                     /translation="MPHATTDDGVKLYYEEAGEGKPLIFVHEYAGDHRSWEPQMRLFS
                     RWYRCIAFNARGYPPSDVPEDPEKYSQARARDDIRAVFDHLEIEKAHVCGLSMGGFAA
                     LHFAITYGARALSLVVGGCGYGAEPAKREQFRAETNAAAEQIEKQGMREVAKRYSLGP
                     TRVQFQNKNPRAWREFAEQLAEHSTLGSALTMRGVQGRRPSLWELVEEMKRIAVPTLI
                     VTGDEDDPCLEPAILMKRSIATAALAVLPSAGHTINLEDPEEFNRVLFNFLAAVDMGT
                     WRRRDPRSTSGGILGMARK"
BASE COUNT          373 a          662 c          691 g          312 t
ORIGIN      
        1 ctgcgctgac cagcggtaac cccgcgaagc gctttggctt gaactccaag ggcgacatcg
       61 cgccgggcct ggacgccgat ctcgcgctcg tcgatccgaa gcacacgtgg accatccacg
      121 caaaggattc cgaatccacc cagggctata cgccgttcga gggcatcgag ctctccgcga
      181 aggtgaaggc cacgttcctg cgcggcagcc tgatttgcga gaacggcaac gtgctcggca
      241 aaccgaaagg ccgttacatg catcgtccga ccccatgaac cttgaactga aaggcagatc
      301 ggtcctcgtc accggcggct cgaagggcat cggtcttgcg tgcgcgaaag cgctcgccgc
      361 cgaaggctgc cgcgtccaca tcgcctcgcg caacaaggaa acgcttgata gggccgcgca
      421 ggcgatcgga ggggaaaccc gcgtgcatgc agtcgacctg cgcgacggca aagcgctgcg
      481 cgcgctcgcc gaggcgtgcg cggacatcga catcctggtg aacaacgccg gcgacatccc
      541 cggtggcacg atcgaggcga tcgacgaggc gaagtggcgg cacgcgtggg agctgaaagt
      601 cttcggctac gtcaacctca cgcgcgaact tttcgcgcgc atgaaggcgc gcaaaagcgg
      661 cgtgatcgtg aacgtcatcg gcatggcggg cgagaaccct tcgttcgagt acgtctgcgg
      721 cagcatggcg aacgcagggc tcggcgcgtt taccaaggcc atggggaagg gatcgagcgc
      781 gttcggcgtg cgcgtgctcg gcgtgcatcc gccggcgacg cgcaccgacc gcatcatccc
      841 gcttatgaaa gccgccgccc gggagaagct cggcgacgaa aaccgctggc aggagctgat
      901 cggcaccggc tccttcgggc agatgatcga gcctgagcag gtggcggaca tcgtgacctt
      961 cctcgcctcg gcgcgggctg gcaagctttc cggagtcatg atcaacctcg gagcctgagc
     1021 gcatgccgca tgccacgacc gacgatggcg tcaagctgta ttacgaggag gccggcgaag
     1081 gcaaaccgct gatcttcgtg catgagtacg cgggtgatca tcgcagctgg gagccgcaga
     1141 tgcggctctt ctcgcgctgg tatcgctgca tagccttcaa cgcgcgcggc tacccgccgt
     1201 ccgacgtccc cgaggatccg gagaagtatt cgcaggcgcg cgcccgcgac gacattcgag
     1261 cggtgttcga tcacctggag atcgaaaagg cgcacgtctg cggtctctcg atgggcggct
     1321 tcgcggcgct gcacttcgcc attacgtacg gtgcgcgcgc gctttcgctc gtggtcggcg
     1381 gctgcggcta tggcgccgag ccggcgaagc gcgagcagtt ccgcgcggag accaacgccg
     1441 ccgccgagca gatcgagaaa cagggcatgc gcgaggtggc gaaacgctac tcgctcggcc
     1501 cgacgcgcgt gcagttccag aacaagaatc cgcgcgcctg gcgcgagttc gccgagcagc
     1561 tcgccgagca ctcgacgctc ggctcggcgc tcaccatgcg cggcgtccag ggacgcaggc
     1621 cttcgctctg ggagctggtg gaggagatga agcgcatcgc ggtcccgacg ctcattgtga
     1681 cgggcgacga ggacgacccg tgcctcgagc ccgcaattct catgaagcgt tccatcgcga
     1741 ccgccgcgct tgccgtgctg ccaagcgcgg gccacacgat caacctcgag gatccagagg
     1801 agttcaaccg ggtgctgttc aacttccttg ccgccgtgga catgggcacg tggcgccggc
     1861 gcgatccgcg ctcgacctcc ggcggcatcc tcggcatggc gagaaagtga cgacgaacgg
     1921 gttcgttcat tacgtcgggg gcggcaagcc gggcctgcgc ctggcggtga aggacaacat
     1981 cgatgtcgcg ggcatgccga gcgccggcgg catggagcct taccgcggcc gcatcgcg
//
LOCUS       DASUNT010000275         2785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_476242, whole genome shotgun sequence.
ACCESSION   DASUNT010000275 DASUNT010000000
VERSION     DASUNT010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476242"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="VFX94_05535"
     CDS             <1..875
                     /locus_tag="VFX94_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX5767679.1"
                     /translation="GAYGNTERRTQFWRQQVKAPGEAKSDLWQIVEFSKRFKVEEVWP
                     PELLKLKPEYRGKTLYDVLFANGQVNKFPEKPNDESRDFGFYLQKGLFEEYAQFGRGK
                     AHDLADFDTYHKVRGLRWPVVDGKETKWRFREGYDPYVQKGEGVRFYGKPDGKAIVIA
                     VPYEPPAEVPDKDYDLWLCTGRVLEHWHSGSMTRRVPELHRAQPEALVFMHPNDAKKR
                     KLLNGQEVKLTSRRGEIRARIEIRGRNEPPEGLVFVPFFDEGRLVNKLTLDATCPISR
                     ETDYKKCAVKVTKV"
     gene            941..1288
                     /locus_tag="VFX94_05540"
     CDS             941..1288
                     /locus_tag="VFX94_05540"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF015828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HEX5767680.1"
                     /translation="MKPIRGKVAIPDTNPADTYRMERHDRTIRRAHKWQPPIIPHHIR
                     GYQITKNVNTCMTCHSKKAAPESGATPVGKSHYLDREGKEHPNISTRRYFCLQCHVPQ
                     FDAEPLVGNTYTP"
     gene            1285..1863
                     /locus_tag="VFX94_05545"
     CDS             1285..1863
                     /locus_tag="VFX94_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEX5767681.1"
                     /translation="MKRWMKRVGPWTIIGLFAAGIVFWGGFNWSFELTNTEPFCISCH
                     EMQENVFKEYQHTVHYTNRSGVRATCPDCHVPKEWGPKIVRKIYASNELLHKVLGSVD
                     TPEKFQAKRLKLAESEWRRMKANDSQECRNCHKYDYMDFSEQGRRAVRTHSRAFDEGK
                     TCIECHQGIAHQLPPIYQEIGAPRAVAATRAP"
     gene            1894..2502
                     /gene="ccmA"
                     /locus_tag="VFX94_05550"
     CDS             1894..2502
                     /gene="ccmA"
                     /locus_tag="VFX94_05550"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HEX5767682.1"
                     /translation="MLEGVDLECERGGRTLFRSLSLAVQAGQSLRIAGANGSGKTSLL
                     RILCGLAAPSAGEVRWQGTPIGELKEDYSRQLVYLGHAPAVKGDLTAAENLSITCTLA
                     GLHTKNSEGHAALERFGLPVQKTAVRYFSQGQRRRAALARLCLSSALPLWLLDEPFSA
                     LDAAGIELQRTLVVEQLGRGGAVVFTTHQDPGIAARHVLDLG"
     gene            2504..>2785
                     /locus_tag="VFX94_05555"
     CDS             2504..>2785
                     /locus_tag="VFX94_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX5767683.1"
                     /translation="MLAAARCIVYRDLLLAVRRPGDVATALLFFVIVASLFPLGIGAE
                     PNLLRAVAPGVIWVAALLSSMLSLGRLFAADYDDGTLEQMLLGAAPLGVV"
BASE COUNT          505 a          899 c          943 g          438 t
ORIGIN      
        1 aaggcgcgta cggcaacacc gagcggcgca cccagttctg gcgccagcag gtgaaggcgc
       61 cgggcgaggc gaagtcggac ctctggcaga tcgtcgagtt ctccaagcgc ttcaaggtcg
      121 aggaggtgtg gccgccggag ctgctgaagc tcaagcccga gtaccgcggc aagacgttgt
      181 acgacgtgct cttcgccaac gggcaggtga acaagttccc cgagaagccg aacgacgagt
      241 cgcgcgactt cggcttctac ctgcagaagg gcctgttcga ggagtacgcg cagttcggcc
      301 gcggcaaggc gcacgacctc gccgacttcg acacctatca caaggtgcgc gggctgcgct
      361 ggcccgtggt cgacggcaag gaaacgaagt ggcgcttccg cgagggctac gacccgtacg
      421 tccagaaagg cgagggggtg cgcttctacg gcaagcccga tgggaaagcg atcgtgatcg
      481 cggtgcctta cgagccgccg gccgaagtgc ccgacaagga ctacgacctg tggctgtgca
      541 ccgggcgcgt gctcgagcac tggcatagcg gctcgatgac gcggcgtgtg cccgagctgc
      601 accgcgcgca gcccgaggcg ctcgtcttca tgcacccgaa cgacgcgaag aaaaggaagc
      661 tcctcaacgg ccaggaagtg aaactcacct cgcgccgcgg cgagatccgc gcgcgcatcg
      721 agatccgcgg gcgcaacgag ccgcccgagg ggctcgtgtt cgtgccgttc ttcgacgagg
      781 ggcgtctcgt caacaagctg acgctggatg cgacctgccc gatttcgcgc gagaccgact
      841 acaagaagtg cgccgtcaag gtgacgaagg tatgaggctg cttctcggtt tcgtactgct
      901 gctgggtttc acggccgccg gtctttcaca agaagaagct gtgaaaccca tccgtggcaa
      961 ggtggcgatc ccggacacca atccggcgga cacgtatcgc atggagcgcc acgaccgcac
     1021 gatcaggcgc gcgcacaagt ggcagccgcc gatcatcccg catcacatcc gcggctacca
     1081 gatcacgaag aacgtgaata cctgcatgac ctgccacagc aagaaggcgg cgccggaaag
     1141 cggcgcgacg ccggtcggca agtcgcacta cctcgatcgc gaaggcaagg agcatcccaa
     1201 tatctccacg cggcgctatt tctgcctgca gtgccacgtg ccgcaattcg acgcggagcc
     1261 gctggtcggg aacacctata cgccatgaag cgatggatga agcgcgtcgg gccgtggacc
     1321 atcatcgggc ttttcgccgc cgggattgtg ttctggggcg ggttcaactg gtcgttcgag
     1381 ctcaccaaca ccgagccctt ctgcatctcg tgccacgaga tgcaggaaaa cgtgttcaag
     1441 gaataccagc acacggtgca ctacaccaac cgttcgggcg tgcgcgcgac ctgcccggac
     1501 tgccatgtgc ccaaggaatg ggggccgaag atcgtgcgca agatctacgc gtcgaacgag
     1561 ctgctgcaca aggtgctcgg cagcgtcgac acgccggaga agttccaggc gaagcgcctc
     1621 aagctcgccg agagcgaatg gcggcgcatg aaggcgaacg actcgcagga atgccgcaac
     1681 tgccacaagt atgactacat ggacttctcc gagcaggggc ggcgcgcggt gcgcacccac
     1741 agccgtgcct tcgacgaggg caagacctgc atcgagtgcc accagggcat cgcgcaccag
     1801 ctgccgccga tctaccagga gatcggcgct ccccgagcgg tggcggcgac tcgagcgccc
     1861 tagcggcagc ccggtttctg caacaattgc gatgtgctgg agggggtcga cctggagtgc
     1921 gagcggggcg ggcgaacgct gtttcgctcg ctatcgcttg ccgtgcaggc cggccaatcg
     1981 ctgcgcatcg cgggggccaa cggcagcggc aagacgagcc tgcttcgcat cctgtgcggg
     2041 ctcgccgcgc cgagcgcggg cgaggtgcgc tggcagggaa ctccgatcgg ggagctgaaa
     2101 gaggattact cgaggcagct cgtctacctc ggccatgcgc cggcggtgaa aggcgacctt
     2161 accgccgccg agaacctgtc catcacctgc acgctcgcgg gcttgcacac caaaaacagc
     2221 gaaggccatg ccgcgctcga gcggttcggc ttgccggtgc agaagacggc tgttcgatat
     2281 ttttcgcaag gacaacgccg ccgggccgcg ctcgcgcggc tgtgcctgtc gagcgcgctt
     2341 ccgctgtggc tgctggacga gccgttctcg gccctcgatg cggcggggat cgagctccag
     2401 aggacgcttg tggtggagca gctcgggcgc ggaggggcgg tcgtcttcac cacgcaccag
     2461 gaccccggca tcgcggcgag gcacgtgttg gacctcggct gacgtgctcg ctgctgcgcg
     2521 ctgcatcgtg taccgcgacc tgctcctcgc cgtgcggcgg ccgggcgacg tggcgactgc
     2581 gctgctcttt ttcgtgatcg tggcgagcct ttttccgctc ggcatcggcg ccgagcccaa
     2641 tctgctgcgc gcggtcgcgc ccggcgtgat ctgggtcgcg gcgctgctct catccatgct
     2701 gtcgctcggg cggctcttcg ccgccgacta cgacgacggc acgctcgagc agatgctgct
     2761 cggcgccgcg ccgctcggcg tggtg
//
LOCUS       DASUNT010000276         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_914113, whole genome shotgun sequence.
ACCESSION   DASUNT010000276 DASUNT010000000
VERSION     DASUNT010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914113"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFX94_05560"
     CDS             complement(<1..407)
                     /locus_tag="VFX94_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX5767684.1"
                     /translation="MRIVERPYVEASRRALLDAGMHPVLARVYAGRNIRSPADLDYPL
                     GGLMPPGSLKGIAAAATLLADAIQQAKRLLIIADYDADGATACAVGVRALKAFGATVD
                     YLVPDRFKLGYGLSPELVDLSAQRRPDLLITVD"
     gene            complement(404..1366)
                     /locus_tag="VFX94_05565"
     CDS             complement(404..1366)
                     /locus_tag="VFX94_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767685.1"
                     /translation="MHCELVVPGLLAGGGASRFVALELLLARGRRSVGEAKPLEGWVQ
                     DAFELGEGAYPAGALTRAAQGADPGEDPWTRADPVHLRVVDDQLVVVPAEAFALQAEE
                     ASALCEALNAHFVGMEFTLSQPRRWCARLAATDETRPAIAAAGTALELPKQAARVLTE
                     IQMVLHAHPVNEAREARGEPAVNSVWLWGAGRAPRARAPWQSVAADDPAVLGAARLAA
                     ARQRALPRSAEAWLERLPDDGRHLAVLDALRAPLALGADTVQEELEKLEREWFAPLLA
                     ALRAARIGMLTLHVPDAAEALSCEIIRADLRRFWRLRQPLKNYT"
     gene            complement(1366..>2143)
                     /locus_tag="VFX94_05570"
     CDS             complement(1366..>2143)
                     /locus_tag="VFX94_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEX5767686.1"
                     /translation="QIPEAPGVYLLYGEGAAPLYIGKSRAMRSRVLQHFIAGARWTGR
                     VRRVEWQRTAGELGALLREANLVKALDPAYNRQLRRPERLHGFAFDGKRLRLAGEDEI
                     DADTLPFVYGVWRSRRAALQALRAVADEHRLCLQTLGFDIKKQGVCLRHQLGRCAGVC
                     AGKESVHAHLARLALALAPLKSREWPHRGPLAVVEADQSRDATEVHVLDRWCYLGTAT
                     SDAEVSELLEARRARFDYDHYRILSRHLSRRGVRVVPLTA"
BASE COUNT          327 a          750 c          766 g          300 t
ORIGIN      
        1 ttatccacgg tgatcaggag atcgggcctg cgctgcgcgg aaaggtcgac cagctccggc
       61 gaaagtccgt accccagctt gaagcggtcg gggacgagat aatcgacggt tgcaccaaaa
      121 gccttgagcg cgcgcacgcc gacggcgcag gcggtggcgc cgtcggcatc gtagtccgca
      181 atgatgagaa ggcgcttcgc ttgctggatc gcgtcggcga ggagcgttgc agccgcagcg
      241 atgcccttca gcgaaccggg cggcatcagc ccaccgagcg ggtagtcgag gtccgcgggc
      301 gagcggatgt tgcgtcccgc gtacacgcgc gcgagcactg gatgcatccc cgcgtcgaga
      361 agcgcgcgac ggctcgcttc gacgtaggga cgctcgacga ttctcatgtg tagttcttga
      421 gcggttgccg cagccgccag aagcgccgca ggtcggcccg gatgatctcg cagcttaacg
      481 cttctgcggc gtccggcacg tgcagcgtca gcatgccgat gcgagcggcg cgcagcgcgg
      541 cgaggagagg agcgaaccac tcacgctcga gcttttccag ctcctcctgg acggtgtcgg
      601 cgccgagcgc gagcggggcg cgcagcgcgt cgagcacggc gaggtggcgg ccgtcgtcag
      661 gcaggcgctc gagccatgct tcggccgagc gggggagcgc gcgctggcgc gcggcggcga
      721 gccgcgcggc gccgagcacc gccggatcgt cggccgcgac cgactgccac ggggcgcgcg
      781 cccggggcgc gcgcccggcg ccccagagcc acacgctatt caccgccggc tcgccgcgcg
      841 cctcgcgcgc ctcgttgacc ggatgggcat gcagcaccat ctgtatttcg gtgagaacgc
      901 gcgctgcctg cttcggcagc tcgagcgccg tgccggcggc tgcgatcgcc gggcgcgttt
      961 catcggtcgc ggctagccgc gcgcaccagc gccgcggctg cgacaaggtg aactccatgc
     1021 caacgaagtg cgcgttgagc gcttcgcaga gcgcgcttgc ttcctcggct tgcagcgcaa
     1081 acgcctcggc cggcaccacg acaagctggt cgtccactac gcgcaggtgc acggggtcgg
     1141 cgcgtgtcca gggatcctcg ccgggatccg cgccttgcgc ggcccgcgtg agcgcgcccg
     1201 ccggataggc gccttcgccg agctcgaaag cgtcctgcac ccagccctcc agcggcttcg
     1261 cctcgcctac gctgcggcgg cctcgcgcga gcagaagctc gagcgcgacg aagcggctcg
     1321 cgccgccgcc ggcgaggagc cccgggacca cgagctcgca gtgcatcagg ccgtgagcgg
     1381 cactacccgg actccgcgcc tgctcaggtg tcgcgaaagg atgcggtagt ggtcgtaatc
     1441 gaaacgcgcg cggcgcgcct ctagtagctc ggacacctcg gcatccgaag tggctgtgcc
     1501 gagatagcac cagcggtcca ggacatgcac ctcggtcgcg tcgcggcttt ggtcggcctc
     1561 cacgaccgca agcgggccac ggtgcggcca ctcgcggctc ttgagcggcg caagcgcgag
     1621 cgccaatcgg gcaaggtgcg cgtgcacgct ctctttgccg gcgcaaacgc ctgcgcagcg
     1681 ccccagctgg tgacgcaggc aaacgccttg cttcttgata tcgaagccca acgtctgcag
     1741 gcaaaggcga tgctcgtcgg ccacggcgcg caatgcctgg agcgccgcgc ggcgcgagcg
     1801 ccacacgcca tagacgaagg gcagggtgtc ggcatcgatt tcgtcctcgc ccgcgaggcg
     1861 cagccgcttg ccgtcgaagg caaaaccgtg caagcgctcg gggcggcgca gctgccggtt
     1921 gtaggccggg tcgagcgcct tgacgaggtt cgcttcgcgc agcagtgcgc cgagctcgcc
     1981 tgcggtgcgc tgccattcga cgcgccgcac gcgccccgtc cagcgcgcgc ccgcgatgaa
     2041 gtgctgaagc acgcgcgagc gcatggcgcg gctcttgccg atgtagagcg gcgccgcacc
     2101 ctcgccgtac agaagataga cgcccggcgc ttccggaatc tgc
//
LOCUS       DASUNT010000277         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_914354, whole genome shotgun sequence.
ACCESSION   DASUNT010000277 DASUNT010000000
VERSION     DASUNT010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914354"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /gene="rplD"
                     /locus_tag="VFX94_05575"
     CDS             <1..228
                     /gene="rplD"
                     /locus_tag="VFX94_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX5767687.1"
                     /translation="DTPKTKLLAQKVKAMGFDEVLVLTEEIDQNLLLSSRNLQNVQVM
                     SVRDANPLALVRSAKVLLTRGAVSRLEELLK"
     gene            240..530
                     /gene="rplW"
                     /locus_tag="VFX94_05580"
     CDS             240..530
                     /gene="rplW"
                     /locus_tag="VFX94_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX5767688.1"
                     /translation="MDRLMNVVLAPVVSEKSTFVADKNRQYVFRVADGASKPQIKAAV
                     ELMFKTKVDSVTVSNVKGKERRFGRTIGRKRSWKKAYVRLAEGQEINFAATE"
     gene            536..1363
                     /gene="rplB"
                     /locus_tag="VFX94_05585"
     CDS             536..1363
                     /gene="rplB"
                     /locus_tag="VFX94_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX5767689.1"
                     /translation="MPLVKVKPTSPGRRALVKVVTPELHKGDPFWPLTRKQTKTVGRN
                     HHGTITMRHKGGGNKQHYRVIDFRRDKDGINARVERLEYDPNRSAHLALLLSADGERR
                     YMIAPKGLQVGAQVVSGAEAPIKTGNALPLRNVPVGTIVHCVEMMPGKGAQLARSAGA
                     GVQVLAREGSYAQLRLRSGEIRKVHIDCRATIGEAGNAEHNLESIGKAGRKRWRGVRP
                     TVRGVAMNPIDHPHGGGEGRTAAAQPPVSPWGVQTKGYKTRRNKRTEVMIVRDRRRA"
     gene            1363..1617
                     /gene="rpsS"
                     /locus_tag="VFX94_05590"
                     /pseudo
     CDS             1363..1617
                     /gene="rpsS"
                     /locus_tag="VFX94_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
     gene            1743..>2060
                     /gene="rplV"
                     /locus_tag="VFX94_05595"
     CDS             1743..>2060
                     /gene="rplV"
                     /locus_tag="VFX94_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX5767690.1"
                     /translation="MMETRASLRGARLSAQKGRLVADLIRGKPVDQAINILAYTPKKG
                     AVLIRKLLESAIANAEHNDGADIDTLKVKTITVERGMFLKRFHARAKGRGNRILKHTC
                     HIYI"
BASE COUNT          413 a          673 c          692 g          282 t
ORIGIN      
        1 gatacgccga agaccaagct actcgcacag aaggtgaagg cgatgggctt cgacgaggtg
       61 ctggtgctga ccgaggagat cgaccagaac ttgctgcttt cgtcgcgcaa cctgcagaac
      121 gttcaggtga tgtcggtgcg cgacgccaac ccgctcgcgc tcgtgcgctc ggccaaggtg
      181 ctgctcaccc ggggcgccgt gtcgcggctg gaggagctgc tcaagtgaag aaatacagca
      241 tggaccggct gatgaacgtg gtgctcgcgc ccgtggtgtc ggagaagagc accttcgtcg
      301 ccgacaagaa ccggcagtac gtgttccgcg tcgccgacgg cgcgtccaag ccgcagatca
      361 aggcggccgt ggagctgatg ttcaagacca aggtggattc ggtgactgtg tcgaacgtca
      421 aaggcaagga gcggcgcttc ggccgcacca tcgggcgcaa gaggagctgg aaaaaagcct
      481 acgtgcgcct ggccgaaggg caggaaatca acttcgccgc cacggagtaa cgccaatgcc
      541 tctcgtcaaa gtcaagccta cttcgcccgg ccggcgcgcg ctcgtcaagg tcgtgacccc
      601 cgagctgcac aagggtgatc cgttctggcc gctcacgcgc aagcaaacga agacggtcgg
      661 ccgcaaccac cacggcacga tcaccatgcg ccacaagggc ggcggcaaca agcagcatta
      721 ccgggtgatc gatttccggc gcgacaagga cggcatcaac gcgcgcgtcg agcggcttga
      781 gtacgaccct aaccgcagcg cgcacctcgc gctcctgctc tccgccgacg gcgagcgacg
      841 ctacatgatc gcgcccaagg gcctgcaggt gggcgcgcag gtggtgtcgg gcgccgaggc
      901 gccgatcaag accggcaacg cgctgccgct gcgcaacgtt ccggtcggca cgatcgtgca
      961 ttgcgtcgag atgatgcccg gcaagggtgc ccagctcgcg cgctccgccg gtgccggcgt
     1021 gcaggtgctc gcgcgcgagg gctcgtacgc gcagctcagg ctgcgctcgg gcgagatccg
     1081 caaggtgcac attgactgcc gcgccacgat cggtgaggcg ggcaacgcgg agcacaatct
     1141 cgagtcgatc ggcaaggcgg ggcgcaagcg ctggcgcggt gtgcgcccga cggtgcgcgg
     1201 cgtagcgatg aacccgatcg accatccgca cggcggcggc gaaggccgca cggcagcggc
     1261 gcagccgccg gtttcgccct ggggagtgca gaccaagggc tacaagaccc ggcgcaacaa
     1321 gcgcactgag gtcatgatcg tccgcgacag gagacgagcc taatggcacg ctcgatcaag
     1381 aagggcccct ttgtcgacca tcacctgatg aagaaggtgg agtcggcgcg cagcaccaac
     1441 gacaagcgcc cgatcaagac ctggtcgcgg cgctcgacca tcgtgcccga gttcgtcggg
     1501 ctgacgatcg ccgtgcacaa cggcaagcag cacatgccgg tttacgtgac cgagaacatg
     1561 gttggccaca agctcggcga gttcgcgctg acgcgcatct tcaagagcca ctctgccgtg
     1621 ggcaagcgca ccgcggaagc ggtcggcgag gctcgccggc cgccgcccgg cacgcagggt
     1681 cccgcagcgg cacccgcggc ggcccctgcc gcagcccccg cggcggctcc tggtgggaag
     1741 aaatgatgga aaccagagca agcctgcgcg gcgcccggct ctcggcgcag aagggccggc
     1801 tggtggccga cctgatccgc ggcaagcccg tggaccaggc gatcaatatc ctcgcttaca
     1861 cgccgaagaa gggcgcggtt ctgatccgaa agctgcttga gtcggccatc gccaacgccg
     1921 agcacaacga cggcgccgac atcgatacgc tgaaagtcaa gacgatcaca gtggagcgcg
     1981 gcatgttcct caagcgcttt cacgcccgag ccaaaggacg cggcaaccgc atcctcaagc
     2041 acacgtgcca tatctacatt
//
LOCUS       DASUNT010000278         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1090046, whole genome shotgun sequence.
ACCESSION   DASUNT010000278 DASUNT010000000
VERSION     DASUNT010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090046"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VFX94_05600"
     CDS             complement(<1..830)
                     /locus_tag="VFX94_05600"
                     /inference="COORDINATES: protein motif:HMM:NF006530.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HEX5767691.1"
                     /translation="MIDVAAAVIQRPDGAFLLAQRPPGKVYAGYWEFPGGKVEHGEPP
                     HRALARELHEELGIDIGPAYPWITRVYTYPHGTVRLNFFRVLDWRNDPHPREDQAIAW
                     QASDAPMAAPMLPANAPVLASLALPVEYAVTDAARYGIAAMLSRLGQRLEQGLKLVQV
                     REPGLGVEQRDLFTSQVIGLAHRHGCKVMVKSAFSGADGIHFKASELASLKERPRGML
                     AAASCHSRAELEQAMELELDFAVLGPVKDKPPALGWARFADLARGASIPVYAIGGLTC
                     S"
     gene            complement(842..1696)
                     /locus_tag="VFX94_05605"
     CDS             complement(842..1696)
                     /locus_tag="VFX94_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5767692.1"
                     /translation="MSKELERILERLDQVLPPPPPPPDWKAGIAFRWRSSGRRGAAGW
                     LQPVRQVHRIKRSDLRGIDKQSERVEQNTRQFLDGKPANNVLLTGARGTGKSSIVKGL
                     LNKYARQGLRLIEVEKNDLIDLPTIVEQVAPRPERFLLFCDDLSFHGAEEGYIALKVA
                     LDGSISTTSENLLIYATSNRRHLMPEYMAENLETKYLGDEIHPGETVEEKISLSERFG
                     LWVTFYPFDQDEYLEIVGRWLESFGSSLGEKAKEEALQWALQRGSRSGRVAYQFARDW
                     AGRHARKR"
     gene            complement(1707..>2043)
                     /locus_tag="VFX94_05610"
     CDS             complement(1707..>2043)
                     /locus_tag="VFX94_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEX5767693.1"
                     /translation="YDECRKVAYAIAHSPLVKTAFFASDPNLGRILAAMGNTGVELSR
                     LELYLDEVMVATGNGRHPAYREEQGVAALKKPEFTVRAVLNRGSAQATVWTCDLSYDY
                     VKINAEYRT"
BASE COUNT          345 a          674 c          654 g          370 t
ORIGIN      
        1 gagcacgtca atccaccgat cgcgtagacc ggaatactcg cgccgcgagc aaggtcggcg
       61 aagcgcgccc atcccagcgc tggcggcttg tccttcaccg gcccgaggac ggcgaagtcc
      121 agctcgagtt ccattgcctg ctctagctcc gctcggctgt ggcacgaggc cgccgcaagc
      181 atcccacgag gtctttcctt cagtgaagcc agctcagaag ccttgaaatg aatgccatcc
      241 gcacccgaga aggccgactt caccattacc ttgcagccgt gacggtgtgc gagcccgatc
      301 acctgagagg tgaatagatc ccgctgttcg acgcccaagc ccggctcgcg cacttgcacc
      361 agcttgagcc cctgctccag ccgctgtccc agccgagaaa gcatcgccgc gatgccgtag
      421 cgcgccgcat cggttacggc gtattcaacc ggcagcgcga gcgaggccag aaccggcgcg
      481 ttggcgggca gcatcggagc agccatgggg gcgtcggaag cttgccacgc gatcgcctgg
      541 tcttcgcgcg ggtgcgggtc gttgcgccaa tcaagaactc tgaagaagtt caggcgcacc
      601 gtaccgtgag gataggtata tacgcgtgtg atccacgggt acgccggccc gatgtcgatg
      661 ccgagctcct cgtgcagctc gcgggcgagc gcccggtgcg gcggctcgcc gtgctccacc
      721 ttgccgccgg ggaattccca gtatcccgcg tagaccttgc ccggcggacg ctgcgcgagc
      781 aggaaagcgc cgtcggggcg ctggatcacc gccgccgcta catcgatcac gtccgccttt
      841 cttacctttt cctggcgtgc ctgccggccc agtcgcgggc gaactggtaa gcgacacggc
      901 cgctgcgcga gcctctttgc agcgcccact gcagcgcctc ctcttttgct ttttcaccga
      961 gagaggagcc aaacgactcg agccaccggc ccacgatctc caggtactcg tcctggtcga
     1021 aggggtagaa cgtcacccat aggccgaagc gctcggaaag cgagatcttc tcctccaccg
     1081 tctcgccggg atggatctcg tcgccgaggt acttggtctc gagattctcg gccatgtact
     1141 cgggcatcaa gtggcggcgg ttggaggtcg cgtagatgag gaggttctcc gaggtcgtcg
     1201 agatcgagcc gtcgagcgcc accttgagcg cgatgtagcc ctcctcggcg ccgtggaagc
     1261 tcaggtcgtc gcagaacagg agaaagcgct cggggcgcgg cgcgacctgt tcgacgatgg
     1321 taggcaggtc gatgaggtcg tttttttcca cctcgatcag ccgcagtccc tgccgcgcgt
     1381 acttgttcag cagccctttc acgatcgagg atttgccggt gccccgcgcc ccggtgagga
     1441 gcacgttgtt cgccggtttg ccgtcgagga actggcgcgt gttctgctcg acgcgctcgc
     1501 tctgcttgtc gatgccgcgc aagtccgagc gcttgatgcg atgcacctgc cgcaccggct
     1561 gtaaccaccc ggcggcacca cggcggccgg agctgcgcca gcgaaacgcg atgcctgcct
     1621 tccagtcggg cggcggcggc ggcggcggca ggacctgatc gagccgctcc agaattcgtt
     1681 cgagttcttt ggacaaggcc aaaagcctac gtacggtatt cggcgttgat tttcacgtag
     1741 tcgtacgaaa gatcgcaagt ccacacggtg gcctgcgcgc ttccgcggtt cagcaccgcg
     1801 cgcacggtga attccggctt cttcagcgcc gcgacgccct gctcttcgcg gtacgcggga
     1861 tgccggccgt taccggtggc caccatcacc tcgtcgaggt aaagctccag ccgggagagc
     1921 tcgacgccgg tattgcccat ggccgcaagg atgcggccga ggttcgggtc ggaggcgaag
     1981 aaggccgttt tcacgagcgg cgaatgcgcg atcgcgtaag ccaccttgcg gcactcgtcg
     2041 taa
//
LOCUS       DASUNT010000279         3649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_652180, whole genome shotgun sequence.
ACCESSION   DASUNT010000279 DASUNT010000000
VERSION     DASUNT010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652180"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VFX94_05615"
     CDS             <1..692
                     /locus_tag="VFX94_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX5767694.1"
                     /translation="KRGALQGVADEGGYWPAFRTNEEGLAELVGAISDAGLEPGADVA
                     IALDIAATQLYRGGGYYLALEDRTLSAEQLHAMWMRWIERYPIVSIEDPFAETDAAAM
                     RAFTKAAGIQVVGDDFFVTDEKRVRAANGACNAVLLKPNQVGTLTETLACWNTAREAG
                     YRAIVSARSGESEDVSIVHLAVGWGVPQIKVGGFARSERMAKWNEGLRIEEALGAKAL
                     PFPAGRLFKSK"
     gene            787..2427
                     /gene="kaiC"
                     /locus_tag="VFX94_05620"
     CDS             787..2427
                     /gene="kaiC"
                     /locus_tag="VFX94_05620"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HEX5767695.1"
                     /translation="MERPAHARTLPKAPTAIAGLDEITGGGLPKGRPTLVCGGPGCGK
                     TLLAAEFLIRGATQFGEPGAFLSFEETGEELAQNVRALGFDLDALVAEKKLALDYLRI
                     EPAEILEAGEYDLEGLFVRLGLAIDSVGAKRIALDTIESLFGGFTNQALLRSELRRLF
                     RYLKDKGVSAVITGERGEGTLTRQGLEEYVSDCVILLDHRVTDQVSTRRLRIVKYRGS
                     THGTNEYPFLIDEHGISVLPVTGLALDHAVSDERVSSGIPRLDTMLGGKGYYRGSSIL
                     VSGTAGTGKTSIAAHFAHETARRGERVLYVAIEESPSQLMRNMRTVGIDLQPLAKKGL
                     LRIHASRPTLHGLEMHLVQMHRLIREFEPSAVIVDPISNFVDGGTRQDAHSMLLRLVD
                     FLKVKRITALFTHVTSGATREAADAAISSLIDTCLLVRDIESAGERNRGLYVLKSRGM
                     KHSNQIREFLITPEGIRLEDVYVGPEGVLAGSMRAAQEAREAAEALALQQEVEAKQRE
                     LDRLRAALELRQRAREAAAKRRGADAAQSGNRKAGKAK"
     gene            2424..2726
                     /locus_tag="VFX94_05625"
     CDS             2424..2726
                     /locus_tag="VFX94_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEX5767696.1"
                     /translation="MTSSQSLSGEKWNLRLYTAGQSPKSLAALANLKRVCEEHLAGRY
                     SIEVVDLMKNPRLAKDDQIVAIPTLVRKLPEPLRRIVGDLSDTERTLVGLQLKQRE"
     gene            2731..3402
                     /locus_tag="VFX94_05630"
     CDS             2731..3402
                     /locus_tag="VFX94_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5767697.1"
                     /translation="MQTLDRDFEQELQQLRQQLEEAEDMRRAITSGEVDAFVVGPAGN
                     ARVLLLANAYQRYRQLVERMQQGAATATPSGQILFANQRFADMLGVQLAQLYTAPLDT
                     YVTPADRARLSSFLSAGARESGIEVQLGRREGAAIPARISVASFADGYATILITDLRP
                     TRWPALALGALDAIRSSLEKVSARLAGDAAGREALNEIGAQVNGLARIVDEMLDVEKP
                     LGRGR"
     gene            complement(3439..>3649)
                     /locus_tag="VFX94_05635"
     CDS             complement(3439..>3649)
                     /locus_tag="VFX94_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5767698.1"
                     /translation="ALVGDPKLVLADEPTGNLDPENARQVLQLLKEAVKRRGAAAILA
                     THSESAAAGCDRRYRLTAQALERLP"
BASE COUNT          638 a         1232 c         1232 g          547 t
ORIGIN      
        1 cgaagcgcgg cgcgctccag ggcgtcgccg acgaaggcgg ctactggccc gcgttcagga
       61 ccaacgagga gggactcgcc gagctcgttg gcgcgatctc ggacgcgggc ctcgagcccg
      121 gcgccgacgt cgcaatcgcg ctcgacatcg cggccaccca gctctaccgc ggcggcggct
      181 attacctggc gctcgaggac cgcacgcttt ccgccgagca gctgcacgcg atgtggatgc
      241 gctggatcga gcgctatccc atcgtatcga tcgaagaccc gttcgccgag accgacgccg
      301 ccgccatgcg tgcctttacc aaggcggccg gcatccaggt cgtcggcgat gatttcttcg
      361 taacggatga aaagcgcgtg cgcgcggcga acggcgcatg caatgcggtg ctgctcaagc
      421 ccaaccaggt cggcacgctg accgagacgc tcgcctgctg gaacacggcg cgcgaagcgg
      481 gttaccgcgc gatcgtgtcg gcgcgctcgg gcgagagcga ggacgtctcc atcgtgcacc
      541 tcgccgtcgg ctggggcgtg ccgcagatca aggtcggcgg cttcgcgcgg tccgagcgca
      601 tggcgaagtg gaacgaggga ttgcgcatcg aggaggcgct cggcgctaag gcgctcccct
      661 tcccggccgg gaggctcttc aagtcgaaat agagcgccac acgtagcgga gcactacatc
      721 gagcttgtcc ggtttcccct tacacggtta agattcgccg ttcgagcgag gaaatttcca
      781 gtgaagatgg aacgcccggc gcacgcgcgt acgctgccga aggcgccgac ggccattgca
      841 ggcctcgacg agatcactgg cggcgggctg ccgaagggcc gcccgacgct agtctgcggc
      901 gggcccggct gcggcaagac gctgctcgcg gcagagttcc tgatccgggg cgccacgcag
      961 ttcggcgaac ccggcgcatt ccttagcttc gaggagacgg gcgaggagct cgcgcaaaac
     1021 gtgcgcgcgc tcggcttcga tctcgacgcg ctggtcgcgg aaaagaagct cgcgctcgac
     1081 tacctccgca tcgagccggc cgagatcctg gaggccggcg aatacgacct cgaggggctt
     1141 ttcgtgcgcc tgggcctcgc gatcgactcg gtcggcgcga agcgcatcgc gctcgacacg
     1201 atcgagtcgc tcttcggcgg gttcaccaac caggcgttgc tgcgctccga gttgcggcgg
     1261 ctcttccgct acctcaagga taagggcgtc agcgccgtga tcacgggcga gcgcggcgaa
     1321 ggcactctaa cgcgccaggg gctcgaggaa tacgtttccg actgcgtgat cctgctcgat
     1381 cacagggtaa ccgaccaggt ctccacacgt cgactgcgca tcgtgaagta ccgcggcagc
     1441 acgcacggca caaacgaata tccgttcctg atcgacgagc acggcatctc ggtgctgccg
     1501 gttacgggcc tggcgctcga ccacgcggta agcgatgagc gcgtctcgag cgggatcccg
     1561 cgcctcgaca ccatgctcgg cggcaagggc tactaccgcg gctcctccat cctcgtctcc
     1621 ggcaccgcgg gcaccggcaa gaccagcatc gccgcgcact tcgctcatga aacggcgcgc
     1681 cgaggcgagc gcgtgctcta tgtggcgatc gaggagtcgc cgagccagct catgcgcaac
     1741 atgcgcaccg tgggaatcga tctccagccg ctcgcaaaga aagggctgct gcgcattcat
     1801 gcctcgcggc ccacgcttca cggcctcgag atgcacctcg tgcagatgca caggttgatc
     1861 cgcgagttcg agccgagcgc ggtcatcgtc gatccgatca gcaacttcgt cgacggcggg
     1921 acccggcagg acgcccactc gatgctcctg cgtctggtgg attttctgaa ggtaaagcgc
     1981 atcaccgcgc tcttcaccca cgtgacgagc ggcgccactc gggaagcggc cgacgcggcc
     2041 atctcgtcgc tcatcgacac gtgccttctg gtgcgcgata tcgagtcggc gggcgagcgc
     2101 aaccgcggcc tctacgtgct caagtcgcgc ggcatgaagc actccaacca gatccgcgag
     2161 tttctcatca ccccggaggg catccggctc gaggacgtct acgtaggccc cgagggcgtg
     2221 ctcgccggat caatgcgcgc ggcccaggaa gcacgcgagg cagccgaggc gctcgcgctg
     2281 cagcaggaag tggaagcaaa gcagcgcgag ctcgaccgcc tgcgcgcggc gctcgagctg
     2341 cgccagcggg cgcgggaggc cgcggcgaaa cgccgcggcg cggacgccgc gcagagcggc
     2401 aaccgaaagg caggcaaggc gaaatgacgt cctcccagtc cctaagcggc gagaagtgga
     2461 acctgcgcct gtataccgcg gggcagagcc cgaagtcgct cgccgcgctt gcgaacctga
     2521 aacgggtgtg cgaggagcac ctcgccggac gctattcgat cgaggtggtg gatctcatga
     2581 agaacccgcg gctcgcgaaa gacgaccaga tcgtcgccat tccaacgctg gtgcgcaagc
     2641 tgccggaacc gctgcgcagg atcgtcggcg acctgtcgga caccgagcgc acgctggtcg
     2701 gcctgcagct caagcagcgc gaatagcgca atgcagaccc tggatcgcga tttcgagcag
     2761 gagctccagc agctgcgcca acagctggaa gaagctgagg atatgcgccg cgccatcacg
     2821 agcggcgagg tcgacgcctt cgtcgtaggc ccggcgggaa atgcccgggt gctgctgctc
     2881 gcgaacgcgt accagcgcta ccgccagctc gtcgagcgta tgcagcaggg tgccgcaacg
     2941 gccacgccga gcggccagat cctcttcgcc aaccagcgct tcgccgacat gctgggcgtg
     3001 cagctcgcgc agctctatac cgcgccgctc gacacctacg ttacgcccgc cgaccgggcg
     3061 cggctatcga gtttcctgag cgccggcgcg cgcgagtcgg gcatcgaggt gcagcttggc
     3121 cggcgcgagg gcgccgccat cccggcgcgg atatcggtcg cctcgttcgc cgacgggtat
     3181 gcgacgatct tgatcacgga cctgcggccg acgcgatggc ctgctctcgc cctgggtgcg
     3241 ctcgacgcaa tccgcagctc gctcgagaaa gtgagcgcga ggctcgccgg cgatgccgcg
     3301 gggcgcgagg cgctgaacga gatcggcgcg caggtgaacg gcctcgcgcg catcgtggac
     3361 gagatgctcg acgtcgaaaa gccactcggg cgcggccgct agtccgcggc gcatgggcag
     3421 cattccgcaa aatcgtcgct agggcagccg ttccaatgcc tgcgcggtaa gccggtagcg
     3481 ccggtcgcat cccgccgccg cggattctga atgcgttgcg aggattgcgg cggcgccgcg
     3541 cctcttcact gcttccttga gcaactgcaa cacctgacgg gcattttccg ggtcgagatt
     3601 ccccgtcggc tcgtcggcaa gcacgagctt cggatccccg acgagcgca
//
LOCUS       DASUNT010000280         3134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_739733, whole genome shotgun sequence.
ACCESSION   DASUNT010000280 DASUNT010000000
VERSION     DASUNT010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739733"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VFX94_05640"
     CDS             <1..846
                     /locus_tag="VFX94_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5767699.1"
                     /translation="LGQILNGIATGMLYALMGLGLSLITGVLNIPNFAHGALFAVGAY
                     LLVTVVGAIGNFWIALLVAPLGVAVLGLLIEYAGIRRLYAAGHDYQLLFTFGLALILN
                     ELIIVIWGPVGISHLPPALLRGGVDLGFTFYPKYRLFVMVMAGLLVAATWLFLERTRY
                     GAIMRAGIENKEMVSLLGIDIHRLFTVAFAIGAFLAGIAGALTAPIRGLSPGMGVDML
                     GIAFVVVALGGLGNLLGAIAAGLLIGVAQAVVAAYWTEASTAVIFAVMALVLLVRPQG
                     LFGIR"
     gene            843..1769
                     /locus_tag="VFX94_05645"
     CDS             843..1769
                     /locus_tag="VFX94_05645"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5767700.1"
                     /translation="MKNPLVLILVAIAALPFIVRPVIATEIWIFAIFGLGLNLLLGYT
                     GLLSFGQSTFFGSAAYVAGWLLKHYGINVFLALGIGVGVGAFSALLVGYLCVQRSGLY
                     FIMLTFALNQLFYFVAYQWTSVTGGEDGMQGVPRPALGGLDFGNPMLYYAFVSVLFLL
                     SLWAMKRIVESPLGKILQAIRENEVRAEAVGFNVPRFKLLAFIIGGAFSGLAGVLYAM
                     LFGIVPLEAISFVFSGNVVFATLIGGSGSLYGPIIGSFVFIWLSESMSVVWARWPLLL
                     GVAFVIVVLFFRGGVVEAWSRFWLWQSSRQKT"
     gene            1742..2491
                     /locus_tag="VFX94_05650"
     CDS             1742..2491
                     /locus_tag="VFX94_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5767701.1"
                     /translation="MAILQTENLSKSFGALAAVKSVTLGIAEGSLHSIIGPNGAGKTT
                     LFNLLTGTFPPSSGRILFEGRDITGTPAHRVAHLGLARSFQRTNVFPAFSLLDNVWVA
                     AFATGPSWQGLLYRKSSFFPEATGRARAALSDVGLLEKEHVAAREISHGEQRQLELAI
                     ALAAAPRVLLLDEPAAGLSPEETRRMVSLVRALKGRYTIVLIEHKIDIIMSVSDRISV
                     MHFGSLIAEGPPEEIQRNPDVRRAYLGGIAA"
     gene            2488..>3134
                     /locus_tag="VFX94_05655"
     CDS             2488..>3134
                     /locus_tag="VFX94_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5767702.1"
                     /translation="MIELERINTFYGLGHILHDLSLSVAEAEVVALLGRNGAGKTTTL
                     RSITGLTPPRTGEIRYKGRNIAGMDAHAVSRLGIALVPETRDIFSFLTARENLAIAQR
                     KSSRWQIETVLERFPGLKTRLANKGRELSGGEQQMLAIARALLTGPDLLLLDEPSQGL
                     APLVVNAVMNTIEELKKERVSMLLVEQNAEMALRLADRVYVIDHGTVVFEGTPAAL"
BASE COUNT          440 a         1105 c          986 g          603 t
ORIGIN      
        1 ctcgggcaga tccttaacgg gatcgcgacc gggatgctgt acgccctgat gggcctcggc
       61 ctgtcgctca tcacgggcgt gctcaacatc ccgaacttcg cccatggtgc gctgttcgcg
      121 gtgggcgcct atcttctggt caccgtcgtg ggggcgatcg gtaatttctg gattgcgctc
      181 ctcgtcgctc cactgggggt tgcggtcctc ggcctgctga tcgagtacgc cggcatccgg
      241 cgtctctacg ccgcgggaca cgactatcag ctcctattca cgttcggcct cgcgctcatc
      301 ctcaacgagc tcatcatcgt gatctgggga cccgtcggca tcagccacct gccgcccgcg
      361 ctgctccgcg gaggggtgga cctcggcttc acgttctatc cgaaataccg cctgttcgtg
      421 atggtcatgg ccgggctgct cgtggccgcg acctggctct tcctggagag aacgcgctac
      481 ggcgccatca tgcgcgcagg catcgagaac aaggagatgg tctcgctcct cggcatcgac
      541 attcaccgcc tgtttaccgt ggcgttcgcg atcggcgcct tcctcgccgg gatcgccggg
      601 gccctcaccg cgccgatccg cggcctttcg cccggcatgg gcgtcgacat gctcggcatc
      661 gcgttcgtcg tcgtcgcgct cggcggactc gggaacctgc tcggcgcaat cgccgccggg
      721 ctcctcatcg gcgtcgcgca ggcggtggtg gcggcgtact ggaccgaggc ttcgaccgcg
      781 gtgatcttcg ccgtgatggc gctcgtcctc ctggtacgcc ctcagggcct cttcggcatt
      841 cgatgaagaa tccgctcgtc ctcatcctgg tcgcgatcgc cgcgctgccc ttcatcgtgc
      901 gcccggtgat cgccaccgag atctggatct tcgcgatctt cgggctcggc ctgaacctgc
      961 tcctcggcta caccggcctg ctctccttcg gtcagtcgac cttcttcggc tcggccgcct
     1021 acgtcgccgg ctggctcctc aagcactacg gcatcaacgt ctttctcgcg ctcgggatcg
     1081 gcgtcggcgt gggcgcgttc tccgcgcttc tcgtcggcta cctctgcgtg cagcgctcgg
     1141 ggctgtactt catcatgctc acgttcgcgc tcaaccagct tttctacttc gtcgcctacc
     1201 agtggacgag cgtcaccggc ggcgaggacg gcatgcaggg cgtcccgcgg cccgcgctcg
     1261 gcgggctcga tttcggcaat ccgatgctct actacgcgtt cgtctcggtg ctcttcctgc
     1321 tctcgctctg ggcgatgaag cgcatcgtcg agtcgccgct cggcaagatc ctgcaggcga
     1381 tccgcgagaa cgaggtgcgc gccgaagcgg tcggcttcaa cgtgccgcgc ttcaagctgc
     1441 tcgctttcat catcggcggc gcattctccg gcctcgccgg agtgctgtac gccatgcttt
     1501 tcggcatcgt gccgctcgaa gcgatcagct tcgtgttctc cggcaacgtc gtgttcgcga
     1561 cgctgatcgg cggctcgggc tcgctctacg gaccgatcat cggctctttc gtcttcatct
     1621 ggctttccga gtccatgagc gtggtgtggg cgcgctggcc ccttcttctc ggcgtggcgt
     1681 tcgtgatcgt ggtgctcttc ttccgcggcg gcgtggtcga ggcgtggtcg cgattctggc
     1741 tatggcaatc ctccagacag aaaacctgag caagtccttc ggcgcccttg cggcggtgaa
     1801 gagcgtgacg ctcggcatag ccgaggggag cctgcattcg atcatcgggc cgaacggcgc
     1861 gggcaagacg acgcttttca acctgctcac cggcacattc ccgccgagct cgggaaggat
     1921 tcttttcgaa ggacgggaca tcaccggcac gccggcgcac cgcgtggcgc atctcgggct
     1981 tgcgcgctcc ttccagcgca ccaacgtgtt cccggcgttc tcgctgctcg acaacgtctg
     2041 ggtcgccgcg ttcgccaccg gcccctcgtg gcagggattg ctataccgga aatcgagctt
     2101 cttccccgag gcgaccgggc gcgcccgtgc ggcgctctcc gatgtcggac ttctggaaaa
     2161 agagcatgtg gcggcccgcg agatctcgca cggcgagcag cgccagctcg agctcgcgat
     2221 cgcgctcgcc gcggcgccgc gcgtgctgct cctcgacgag cccgcggccg ggctctcgcc
     2281 cgaggaaacg cgccggatgg tctcgctcgt gcgcgcgctc aagggccgct acacgatcgt
     2341 cctcatcgag cacaagatcg acatcatcat gagcgtctcg gaccgcatct cggtgatgca
     2401 ctttggctcg ctcatcgccg aggggccgcc ggaggagatc cagcgcaacc cggacgtgcg
     2461 ccgcgcctac ctcggcggca tcgccgcatg atcgagctcg agcgcatcaa cacgttctac
     2521 ggcctcggcc acatcctgca cgacctctcg ctcagcgtcg cggaggcaga agtcgtcgcg
     2581 ctgctcggac gcaacggcgc cggcaagacc accacgctgc gctccatcac cggcctcacg
     2641 ccgccgcgca cgggagagat ccgctacaag ggccgcaata tcgccggcat ggacgcgcac
     2701 gcggtgtccc gcctcggtat cgcgctcgtg cccgagacgc gcgacatctt cagcttcctg
     2761 accgcgcggg aaaacctggc gatcgcccag cgcaagagct cgcgctggca gatcgaaacc
     2821 gtgctcgagc gcttcccggg gctgaagacc cggctcgcga acaagggacg cgagctctcc
     2881 ggcggcgagc agcagatgct cgcgatcgcg cgtgcgctcc tcaccggccc ggacctgctg
     2941 ctcctcgacg agccctcgca gggcctcgca ccgctggtcg taaacgcggt catgaacacc
     3001 atcgaggagt tgaagaagga gcgcgtcagc atgctgctcg tcgagcagaa cgcggagatg
     3061 gcgctgcggc tcgccgaccg cgtctacgtc atcgaccacg gcaccgtggt gttcgaaggc
     3121 acgcctgccg cgct
//
LOCUS       DASUNT010000281         2317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_564509, whole genome shotgun sequence.
ACCESSION   DASUNT010000281 DASUNT010000000
VERSION     DASUNT010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564509"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VFX94_05660"
     CDS             complement(<1..908)
                     /locus_tag="VFX94_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5767703.1"
                     /translation="MKYWLSTALVFGVALAVALSPVNEYFFYAGYVVLTFVVLATAWN
                     ILGGYAGYVNFGTGAFFGLGSYSAVVLFKALGAPLAVQIVAAAAVGGALGFGVGMLTL
                     RLRGIFFAIATVAILFITETLMVNWRYVGGATGLQLLRPDVIAPFDSYTRMLFVVMAF
                     LAVLAVSVARYVQVSWIGRGLRAIRDSEEAAECSGVPTLKLKLFACTVSGALMGAAGA
                     PMPMYLSFIEPASSFSLNWGVSALAMPMIGGTAHWIGPVVGALLLGSIQQIVTVTISN
                     EINVLVVGVLLVLFVVLAPDGVVGLF"
     gene            complement(905..1780)
                     /locus_tag="VFX94_05665"
     CDS             complement(905..1780)
                     /locus_tag="VFX94_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5767704.1"
                     /translation="MMFSLDLLFEAVLFGILLGCFYAAVSIGLSVSFGLLDVPHVAHP
                     ALMVFGSYCTYIVAGWGLDPILAGVILMPAFFVLGILIYRFYYETFERRGTEAGVRGL
                     AFFFGLAFIIEVGLILLFGVDQRTVQAPYIGSSLALGEYRMPYRMLVAFGVALVLTFA
                     LTLYLSTTFTGRAIKAVAQDEPALRLMGANPVRIKQWAFGIATGVSALAGAMLIIVGP
                     VEPALDRLYIGRTFCVVVLAGLGSMTGTLAAGLILGIAESVVLMMFGASWAPAVAFGL
                     LLIVLGVRPQGLFGR"
     gene            complement(1812..>2317)
                     /locus_tag="VFX94_05670"
     CDS             complement(1812..>2317)
                     /locus_tag="VFX94_05670"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5767705.1"
                     /translation="QVQVFGGGMVGLQFTPIMTSLGSLLNGVLNYNSYVPGMKYPGIE
                     DFLARYAKRAQEAKVDPLGFYLPPFNYAIGQMYEQAINATKSLDHKQLASYLRSNELK
                     TVVGPIRYDKMGEWANPRVVQAQFRGVVDKDLEQFRKPGTQVVIYPDDHKTGEVITPF
                     EKARTAK"
BASE COUNT          465 a          755 c          784 g          313 t
ORIGIN      
        1 ctgaacaacc ccacgacccc atccggagcc agcacgacga agaggaccag cagcacgccc
       61 accaccagca cgttgatctc gttcgagatg gtgacggtga cgatctgctg gatcgagccg
      121 agcagcagcg cgccgaccac cgggccgatc caatgcgcgg tgccgccgat catcggcatg
      181 gcgagcgccg atactcccca gttcaggctg aagctcgagg ccggctcgat gaacgagagg
      241 tacatcggca tcggcgcgcc cgcggcgccc atcagagcgc ccgacacggt gcaggcgaag
      301 agcttgagct tcagcgtcgg gacgcccgag cactcggccg cctcctcgga gtcgcggatc
      361 gcgcgcagcc cgcggccgat ccacgacacc tggacgtagc gcgcgaccga cacggcgagc
      421 accgcgagga aggccatgac gacgaagagc atgcgtgtgt aggagtcgaa cggcgcgatc
      481 acgtccggcc gcaggagctg cagcccggtg gcgccgccga cgtagcgcca gttcaccatg
      541 agcgtctcgg tgatgaagag gatggccacc gtcgcgatgg cgaagaagat gccgcgcagc
      601 cgcagcgtca gcatgccgac gccgaagccg agcgcgccgc cgacggccgc ggccgccacg
      661 atctggactg cgagcggcgc gccgagcgcc ttgaagagca ccaccgcgct gtaggacccg
      721 aggccgaaga aggctccggt gccgaaattg acgtacccgg cgtagccgcc gaggatgttc
      781 cacgcggtgg cgagcaccac gaaggtcagc accacgtagc ccgcgtagaa gaaatattcg
      841 ttcaccgggg atagcgccac ggcgagcgcc accccgaaga ccaaggccgt gctgagccag
      901 tacttcatct tccgaacagc ccctgcggcc gcacaccgag cacgatcaga agcagcccga
      961 atgcgaccgc gggcgcccac gaggcgccga acatcatgag cacgaccgat tcggcgatgc
     1021 cgaggatgag gccggcggca agcgtgccgg tcatgctgcc caggccggcc agcacgacga
     1081 cgcagaaggt gcggccgatg tagagacgat ccagcgccgg ctcgaccggt cccacgatga
     1141 tcagcattgc gcccgcgagc gcggacacgc cggttgcgat gccgaacgcc cactgcttga
     1201 tgcgcacggg gtttgcgccc atcaggcgca gcgcgggctc atcctgcgcg accgccttga
     1261 tcgcccggcc ggtgaaggtc gtggacagat acaaggtcag cgcaaacgtc aaaacgagcg
     1321 caacgccgaa ggcaacgagc atgcggtacg gcatgcggta ctccccgagc gcgaggctcg
     1381 agccgatgta cggggcctgc accgtgcgct ggtcgacgcc gaaaaggagg atcaggccga
     1441 cctcgatgat gaaggcgagg ccgaagaaga acgcaaggcc gcgcaccccc gcctcggtgc
     1501 cgcggcgctc gaaggtctcg tagtagaagc ggtagatgag aatgccgagc acgaagaacg
     1561 ccggcatcag gatcacgccg gcgaggatcg ggtcgaggcc ccacccggcg acgatgtagg
     1621 tgcagtacga gccgaacacc atgagcgccg ggtgcgcgac gtgcggcacg tcgagcaggc
     1681 cgaacgacac cgagagcccg atgctgaccg cggcgtagaa gcagccgagc aggatgccga
     1741 acagcacagc ctcgaacagc aggtcgaggg agaacatcat gcggcctgct gttctcggaa
     1801 aatcggcggt tttatttcgc cgtgcgcgcc ttttcgaacg gggtgatgac ttcgcccgtc
     1861 ttgtggtcgt ccgggtagat cacgacctgc gtgccgggct tgcggaattg ctccaggtcc
     1921 ttgtcgacca cgccgcggaa ctgcgcctgc acgacgcgcg ggttggccca ttcgcccatc
     1981 ttgtcgtagc ggatcgggcc aaccaccgtc ttcaattcgt tcgagcgcag gtagctcgcg
     2041 agctgcttgt ggtcgaggct cttggtggcg ttgatcgcct gctcgtacat ctgaccgatg
     2101 gcgtagttga acggcggcag gtagaagccg agcgggtcca ccttcgcctc ctgcgcgcgc
     2161 ttggcatagc gcgcgaggaa gtcctcgatc ccgggatact tcatgccggg cacgtaggag
     2221 ttgtagttga gcacgccgtt caggaggctg ccgagcgagg tcatgatagg ggtgaactga
     2281 agaccgacca tgccgccgcc gaacacctgc acctgcg
//
LOCUS       DASUNT010000282         2872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_38472, whole genome shotgun sequence.
ACCESSION   DASUNT010000282 DASUNT010000000
VERSION     DASUNT010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38472"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFX94_05675"
     CDS             <1..235
                     /locus_tag="VFX94_05675"
                     /inference="COORDINATES: protein motif:HMM:NF022007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEX5767706.1"
                     /translation="SVVYVYGEVQKGGAYRIEPDMTVMQAISLAGGITPRGSESRVQI
                     RRKGSDGKWKQFSVKLTDAVSPDDVIYVREGLF"
     gene            253..1692
                     /locus_tag="VFX94_05680"
     CDS             253..1692
                     /locus_tag="VFX94_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein EpsF"
                     /protein_id="HEX5767707.1"
                     /translation="MLDRIQILPALQARWRSALIVWVGIVVAVAALTLAMPARYEATA
                     ALVIEMNAADPMRKGEVFRPSGSVSSYLATQVDVIKSEAVALAALRRLELHKDPSLQE
                     RWREETQGRGDFESWLATGLLNGLAVTPSRESNLVTVSFTSQDPQFAAAIVNAFVQSY
                     MDTTLQMRAGPARQFSTFFQERATSLRQRLDEAKARLTAYEQKHGLIVSDAPNEHDVD
                     NARLTELTSQLVKLQDEATDAANRRRQAAAGADRMREVRSDPEVMDLTKELAARESEL
                     AKLKSQFGDRHPAVLEVQQAITQLRASLSTARQRAAATFDVPVTSNEARIAEVRKAIE
                     QQRAVVMQRRSQRNAAAGLLRDVENAQKAYDAVLQRASETALEAQNTTQPDVSVVKAP
                     TPPAKASSIYLLINVFVAGFLAPLFAVLYAVFREARDRRVRTIGDVTGLLQQPLLLML
                     PDGERQTGRRSLEAQRRLVSAQRRLFAPR"
     gene            1700..2596
                     /locus_tag="VFX94_05685"
     CDS             1700..2596
                     /locus_tag="VFX94_05685"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX5767708.1"
                     /translation="MNLPDRPIAQEQSTAPDGERSLAPLIGELLSEACGLGKPQVEQI
                     LAYARKTGMRFGEAAVALRLVDRKDVLEALYQQFQYTPGFARQQGRTELVTAADRFGD
                     QAEAFRELRSRLLIEVLRDGPRCALAILSPDAGDGKTYLAANLAVSFSQLGERTLLID
                     ADVRTPRQHHVLGVKQNGVGLSSVLAGFTDAATLVHEVPGLPHLYFAPAGPIPPNPVE
                     LMQRPRFGQLVRELLEEFDHVIVDTPAAVRGPDARVIAARCGAALIVARKSRSRLAQL
                     EGLLSALARGPAQIAGVVMNEH"
     gene            2647..>2872
                     /locus_tag="VFX94_05690"
     CDS             2647..>2872
                     /locus_tag="VFX94_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767709.1"
                     /translation="MQWPDGAATSETRLVARERLLTAGILFAMLGLTVLDHFGLRLTE
                     RASIPAGMMAMYALVAAMLLSGAAQLNFRGA"
BASE COUNT          501 a          961 c          944 g          466 t
ORIGIN      
        1 ttccgtggtc tacgtttacg gcgaagtgca gaagggcgga gcgtaccgaa tcgagcccga
       61 catgacggtc atgcaggcga tttctctcgc cggcggcatc acgccgcgcg gcagcgagag
      121 ccgggtgcag atccgccgca agggaagcga cggaaaatgg aagcaattca gcgttaagct
      181 taccgatgcg gtttcgcccg acgatgtcat ctacgtccgt gaaggcctat tttgacgtga
      241 ggcacgaggc gcatgcttga ccgaattcaa atcctgcctg ccctgcaggc gcgctggcgc
      301 tccgcgctaa tcgtttgggt cggcatcgtg gtggccgtcg cggcactcac cctcgccatg
      361 ccagcgcgat atgaagccac cgcggccctc gttatcgaaa tgaacgcggc cgatcccatg
      421 cgcaagggcg aagtcttcag gccctcgggc agcgtctcga gctacctcgc cacgcaggtc
      481 gacgtcatca agagcgaggc ggttgcgctg gctgcgttac gccgtcttga actgcacaag
      541 gatccctctt tgcaggaacg gtggcgggaa gagacgcagg ggcgcggcga cttcgagtcg
      601 tggctggcca ccggcctcct gaacggcctg gcggtcacgc cctcgaggga gtcgaatctg
      661 gtgaccgtga gctttacatc gcaggaccca cagtttgccg ccgccatcgt gaacgccttc
      721 gtgcagtcct acatggatac aacgcttcag atgcgcgccg ggccggcacg ccagttcagt
      781 acatttttcc aagagcgggc cacgtcgctc cggcagaggc tcgacgaggc aaaggcgcgc
      841 cttactgcct atgagcagaa gcacggactg atcgtcagcg acgctcccaa cgagcacgac
      901 gttgacaacg cccggctcac cgagctcact tcgcagctgg tgaagctcca ggacgaggcg
      961 actgacgcgg ccaaccggcg gcggcaggcg gcggccggcg ccgaccggat gcgcgaggtg
     1021 cgcagcgacc cggaggtgat ggacctcacc aaagagctcg ctgcacggga aagcgagctc
     1081 gcgaaactca agtctcagtt cggtgaccgg catcccgccg tcctcgaggt gcaacaagcg
     1141 attacccaac tgcgcgcgag cctttccacc gcgagacagc gggcggccgc gacgtttgac
     1201 gtgcccgtga catcgaacga agcccgcatt gccgaagtgc gcaaagcgat cgagcagcag
     1261 cgcgcggtgg tgatgcagcg caggtcacag cgcaacgccg cggcaggctt gctgcgggac
     1321 gtcgaaaacg cgcagaaggc ctatgatgca gttttgcagc gtgccagcga gaccgccctt
     1381 gaggcgcaga acaccaccca gccggacgtc tccgttgtca aggcgcctac gccgcccgcc
     1441 aaggcctctt cgatctacct gctgatcaac gtgttcgttg cggggtttct tgcgccgctc
     1501 tttgccgttc tgtacgcggt cttccgtgag gctcgtgacc gacgggtgcg caccatcggc
     1561 gatgtgaccg gcttgctgca gcagccactt ctgctaatgc tgcccgacgg tgaacgccaa
     1621 acggggcggc ggtcgctgga agcgcagcgg cgcctcgtct ctgcgcaacg tcggctcttc
     1681 gctccgcgat aagcgcgcca tgaaccttcc cgatcgcccc atagcgcagg agcaaagcac
     1741 agcgccggat ggcgagcgtt ccctcgcgcc tctcatcggc gagctgctga gcgaagcctg
     1801 cgggctcgga aagccgcagg tcgagcaaat tctcgcctac gcgcgcaaga ccggcatgcg
     1861 gttcggcgag gccgcggttg ccttgcgcct cgtcgaccga aaggatgttc tcgaggcgtt
     1921 gtaccagcag ttccaataca cgcccggttt cgcacgtcag cagggcagga ccgagctcgt
     1981 gaccgcggcc gaccgcttcg gcgaccaggc ggaagcgttt cgagaactgc gcagccggct
     2041 gctgatcgaa gtcctgcgcg acgggccgcg ctgcgcgctc gccatcctga gcccggacgc
     2101 gggcgacggc aagacgtacc tcgccgccaa cctggccgtg agcttcagtc agctcgggga
     2161 gcgcacgctt ctcatcgacg ccgacgtccg cacgccgcgc cagcatcacg tgctcggcgt
     2221 aaagcagaac ggggtcgggc taagcagcgt gctggccggt ttcaccgatg cggccacgct
     2281 cgtccatgag gtaccggggc tgccgcacct ctatttcgcg cccgcgggtc ccatcccgcc
     2341 gaatcccgta gagctgatgc agcgccctcg cttcggccag ctggtgcgcg agctgctgga
     2401 ggaattcgac cacgtgattg tcgacacacc ggcggcggtt cgcggcccgg acgcccgcgt
     2461 catcgccgcg cggtgcgggg ccgcattgat cgtcgcgcgg aaaagccgca gccgcctcgc
     2521 ccagctggaa ggcctcctca gcgcgctcgc ccgcgggccc gcccagatcg ccggcgtggt
     2581 catgaacgag cactgagccg cgccacgatg agctcgtggc aggctcggcc ggcgtggcag
     2641 gcacccttgc agtggccgga cggcgccgcg acgtcggaaa cccgcctcgt tgcccgcgag
     2701 cggctgctca cggcgggaat tctgttcgcc atgctggggc tgacggtgct cgatcacttc
     2761 ggattgagac tgacggaaag ggcctccatt cccgccggca tgatggccat gtacgcgctg
     2821 gtggccgcga tgctcctcag cggcgcggcg cagcttaact tccgcggagc ct
//
LOCUS       DASUNT010000283         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_652617, whole genome shotgun sequence.
ACCESSION   DASUNT010000283 DASUNT010000000
VERSION     DASUNT010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652617"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2142)
                     /gene="mutS"
                     /locus_tag="VFX94_05695"
     CDS             complement(<1..>2142)
                     /gene="mutS"
                     /locus_tag="VFX94_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX5767710.1"
                     /translation="GTVSTSSRPSADLPGTTAMMQQYLRIKAEHPDILVFYRMGDFYE
                     LFYDDAEKGSRLLDLTLTARGASAGAPVKMAGVPVHSVEQYLAKLVKLGESVAICEQI
                     GDPATSKGPVDRKVTRIVTPGTLTDAELLDDKADNILLSLHRDKTSVGLAWLSLASGA
                     LRVAEVAPQALENELRRIGPAEVLTSHDIHLNGYFATRLPEWHFDIESGRNKLLKQLG
                     AATLAGYGCDDMTLAIGACGALLDYAAKTQGQALAHVTAVSAERAGEYVRLDAATRRN
                     LELTETLRGEPAPTLFSLLDECATGMGSRLLRHWLHHPLRDQQAVGKRHDAVEALRET
                     SVQKPLKRFSDVERITARLALKSVRPRELAGLRDSLKLLPELRQTLPDASALLRELVG
                     DLATPEEALFILEHAVHAEPSARVVDGGVIADGYNADLDELRALQSNAGAFLVEFEKR
                     EKERTAIPNLRVAYNHVHGYYIEVTNSHAGKVPDDYRRRQTLKNAERYITPELKQFED
                     KALSARDRALALEKSLYEDILGQLQAHLPSLQKIARALAQTDVLSNFASVATKRDYAR
                     PQLTGELCIDIEAGRHAVVEGEIENFIANDTRLSPTRRFLLITGPNMGGKSTYMRQVA
                     LIVLLAHIGSFVPARAARIGPIDQVFTRIGAADDLAGGRSTFMVEMTESAAILHNATD
                     RSLVLMDEVGRGTSTFDGLALAWAIARHLVEK"
BASE COUNT          339 a          681 c          719 g          403 t
ORIGIN      
        1 cttctcaacc agatgacggg cgatcgccca cgccagtgcg agcccgtcga aggtggaagt
       61 gcctctaccc acctcgtcca tgagcacgag gctcctgtct gtggcgttgt gcaggatagc
      121 cgcactctcg gtcatctcca ccatgaaggt cgagcgtccg ccggcgagat cgtcggccgc
      181 gccaatgcgc gtgaagacct ggtcgatcgg tccgatgcgc gcagcgcgtg ccggtacgaa
      241 cgagccgatg tgcgcaagaa gcacgatgag cgcgacctgg cgcatgtagg tcgacttgcc
      301 gcccatgttc gggccggtga tgagcaggaa gcgccgcgtg ggcgagaggc gtgtgtcgtt
      361 cgcgatgaag ttctcgatct cgccctcgac caccgcgtgg cgcccggcct cgatgtcgat
      421 gcagagctcg ccggtgagtt gcggcctggc gtagtcgcgt ttggtcgcga ccgaggcgaa
      481 gttcgacagc acatcggtct gcgcgagcgc tctggcgatt ttctgcaggg atggcaggtg
      541 cgcctgcagc tggcccagaa tgtcctcgta tagcgacttc tcgagcgcca gagcgcgatc
      601 acgggccgaa agcgccttgt cctcgaactg cttcagctcg ggggtgatgt agcgctcggc
      661 gttcttcagc gtctggcgcc gccggtagtc gtcgggaact ttgccggcgt gcgagttggt
      721 gacctcgatg tagtagccgt gcacgtggtt gtacgcgacg cgcaggttgg ggattgcggt
      781 tctctccttc tctcgcttct cgaattcgac caggaacgcc ccggcgttgg attgaagcgc
      841 tcgcagctca tcgaggtctg cgttgtagcc gtcggcgatg acgccgccgt cgaccacgcg
      901 agccgatggc tcggcgtgca cggcatgctc gaggatgaag agcgcttctt ccggggttgc
      961 gaggtcgccg accagctccc tgagaagcgc gctcgcatcc gggagcgtct gccgcagctc
     1021 cggcagcagt ttgaggctgt cgcgcaagcc cgcaagctcg cgcggccgca cgctcttcag
     1081 cgcgaggcgg gcggtaattc gctcgacgtc cgaaaagcgc ttgagcggtt tctggacgct
     1141 cgtctccctt agcgcttcga cggcatcgtg gcgcttgccc acggcctgct ggtcgcgcag
     1201 tggatggtgc agccagtgcc gcagcagccg gctgcccatg ccggtcgcac actcgtcgag
     1261 cagcgaaaag agcgtcggcg caggctcgcc gcgcagcgtc tcggtgagct cgaggttgcg
     1321 ccgcgtggca gcgtcgaggc gcacgtactc gccggcgcgc tcggcgctta ctgcggtgac
     1381 gtgcgcaagc gcctggccct gcgtcttcgc cgcatagtcg agaagcgcgc cgcaggcccc
     1441 gatcgcgagc gtcatgtcgt cgcagccgta gccggcgagc gttgctgcgc cgagttgctt
     1501 gagcagcttg ttcctgccgc tctcgatgtc gaaatgccac tcgggcaggc gcgtcgcgaa
     1561 gtagccgttc aaatgaatgt cgtgcgaagt caatacctcg gcagggccga tgcgccgaag
     1621 ctcgttttcc agcgcctgcg gcgccacctc cgccacccgc agcgctccgc tcgcaagcga
     1681 aagccaggca aggcccacgc ttgtcttgtc tctatgaaga gacaaaagaa tattgtcggc
     1741 cttgtcatcg agcagctcgg cgtcggtcag cgtcccggga gtgacgatgc gggtcacttt
     1801 gcggtcgacc ggtcctttcg aagtcgccgg atcgccgatc tgctcgcaga tcgcgaccga
     1861 ctcgccgagc ttcacgagct tggccaggta ctgctcgacc gagtgcaccg gcacgccggc
     1921 catcttcacc ggtgcgccgg ccgaggcgcc gcgcgcggtg agcgtaaggt ccagcagccg
     1981 cgagcctttc tcggcgtcgt cgtagaagag ctcgtaaaaa tcgcccatcc gatagaacac
     2041 caggatgtcg ggatgctcgg ccttgatgcg caggtattgc tgcatcatgg cagtggtgcc
     2101 cggaagatct gctgaaggtc tcgaactagt gctgaccgtt cc
//
LOCUS       DASUNT010000284         5091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_739923, whole genome shotgun sequence.
ACCESSION   DASUNT010000284 DASUNT010000000
VERSION     DASUNT010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739923"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFX94_05700"
     CDS             complement(<1..516)
                     /locus_tag="VFX94_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HEX5767711.1"
                     /translation="MERSFKQEVAKLKLGEGETFHGEGILAVTKALLQSGVSYVGGYQ
                     GAPVSHVMDVLNDARDILDELGVHVESNANEAGAAAMLGASINYPMRGAVAFKSTVGT
                     NVASDALSNLASAGVKGGTLLVLGEDYGEGASIIQERTHAFAMKSQIWLLDPRPNLPK
                     IVEMVEKGFELS"
     gene            complement(521..1837)
                     /locus_tag="VFX94_05705"
     CDS             complement(521..1837)
                     /locus_tag="VFX94_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX5767712.1"
                     /translation="MTWWLSAIVLFGVLTALFLVGLPAAFAFFGINIAGAWFYLGKEA
                     GLVQLIRNGIVSITSFSLTPIPFFILMGEVLFHTGVAMKAIDAIDRVIWRVPGRLSVV
                     AVVAGTVFSAISGSTIATTALLGSLLLPQMLRRGYHPSMAMGPIMAIGGVDMLIPPSA
                     LTVLLGSLAGISISQLLIAGIVPGVLLSLMFVGWIVLRALADPSLAPSFEHEEMALWE
                     RLNPLVLYVMPLVLIFVVVVAAMAAGWATPTEAAAIGAVATVAVAWIYRALTLSNLMK
                     ALMGTASISAIILFIIIGATTFAQILTFSGATNGMVEAVRNAGFGSWTVLIAMMLVLL
                     LLGCFVDQVSMMLICLPFFMPLVALYGFDPVWFGVLFLICMQLGLLTPPFGMLLFTMK
                     SVAPKEIAMRQVWHAVTPYVVMGLGALIAVMVFPPLATWLPKLLFR"
     gene            complement(1848..2303)
                     /locus_tag="VFX94_05710"
     CDS             complement(1848..2303)
                     /locus_tag="VFX94_05710"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX5767713.1"
                     /translation="MKLYVSLLRGLAAVAAVLLGAMALLVTLDVVMRNVGLGTLAWVN
                     EVSEYSLPVATLLMAPWLLHRNEHVRLDALLVSLPPGAARALEKACDVIGVAICAVFI
                     WYGIRLILDSSRIGSMVVKTLAIPEWWQYALVPVCFSLLAVEFARRLRQ"
     gene            complement(2300..3253)
                     /gene="dctP"
                     /locus_tag="VFX94_05715"
     CDS             complement(2300..3253)
                     /gene="dctP"
                     /locus_tag="VFX94_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX5767714.1"
                     /translation="MKKTLMLLLLAAAPLAAQAQETTLRVVSGFAENTQYVKNLMPVI
                     QALNAEGKGRLQLNFIGGPKAMPPFEVGNAVRTGVVDMALTTGAFYTNIMPEADALKL
                     TQVSAQELRKNGGYDLINKIWNEKANMQYLGRVIDYTPFHLYLTKKIDKPDLTGLKIR
                     ITPVYRDFFQALGATVMQTAPGEVYTALERGVVDGYGWPINGIFDFNWHEKTKFRVDP
                     GFYNAEVSLVMNLDKWKSLSGAQRELLMKHVIALENANDSWKKVNEEDIRKQKEAGIQ
                     VISFDAATAKQYYAKAYDVAWENAIKASPQYGPQMRKLFSK"
     gene            3321..4241
                     /locus_tag="VFX94_05720"
     CDS             3321..4241
                     /locus_tag="VFX94_05720"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5767715.1"
                     /translation="MIWITAMNVQDIDLNLLRVFDAVLHEKGVTPAAARLGLTQPAVS
                     NALARLRKAFGDALFVRTPRGVDATPFARELAEPVRQALALLESALAHGPGFDPATST
                     RAFRFYMSDLGQVEFLPPLVERVQRVAAGVRLEAVALDLEHIGDALAAGGLDLAVGFL
                     PGLGPPLRRRALFRDPYVCLVRADHPLVAKGLTRRRFLEASHALVTYRGGGHRVIEEA
                     LERAGIARRIALRVPHFTVVPMVLERTDLVLTLPARVARVYERRGNVRSLTPPVSIPQ
                     AEVAVHWHERFDGDPGNRWLREQVIELFSG"
     gene            complement(4252..>5091)
                     /gene="dctP"
                     /locus_tag="VFX94_05725"
     CDS             complement(4252..>5091)
                     /gene="dctP"
                     /locus_tag="VFX94_05725"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX5767716.1"
                     /translation="IFVRFVEHLNREGKGLVQVKLIGGPDAIPTFEQGNAVKTGVVDM
                     ASVAPTFYTNFCPECDAQILAPLQTPKLKKTAFWTALDKYTVQKVNAHLLTAYGDGIG
                     FHIWTNKPVPNAGSFKTMRLRTTPNYQPLFAALGATQVQTAPGEVLTALERGVVDGYG
                     WPGIGMFDMGWGAHTKFRIDPSFYNVVVNVLVNHDKWKSLRGDQRAFLNRMADWLETE
                     NVRWVAERRAADDKRLKDAGIQVVDLGPGHRKLAYEAYWDALAKRAPEPVKELRAASD
                     ALR"
BASE COUNT          877 a         1730 c         1632 g          852 t
ORIGIN      
        1 cgagagctcg aagccctttt ccaccatctc cacgatcttc ggcaggttgg ggcgcgggtc
       61 gagcagccag atctgcgact tcatcgcgaa cgcgtgcgtg cgctcctgga tgatggacgc
      121 gccctcgccg tagtcctcgc ccaggacgag aagagtcccg cccttgacgc cggccgatgc
      181 gaggttggaa agcgcgtcgg aggcgacgtt agttcccacg gtggacttga acgcgaccgc
      241 gccgcgcatt gggtagttga tcgaggcgcc gagcatcgcc gcggcgcccg cttcgttcgc
      301 gttcgactcg acgtgcacgc cgagctcgtc caggatatcg cgcgcgtcgt tcagcacgtc
      361 catgacgtgc gacacgggcg cgccctggta gccgccgacg taggacacgc ccgactgaag
      421 aagcgccttg gtcaccgcca ggataccttc cccgtggaag gtctcgccct cgccgagttt
      481 cagcttcgcg acttcctgct tgaacgagcg ttccatgggc ttacctaaac agcagcttcg
      541 gcagccaggt ggcaagcggt ggaaacacca tcaccgctat cagcgcgccc aagcccatca
      601 cgacatacgg cgttaccgca tgccacacct ggcgcatggc gatctccttc ggcgccacgc
      661 ttttcatcgt gaaaagcagc atgccgaacg gcggcgtgag cagccccagc tgcatacaga
      721 tgaggaaaag cacgccgaac cacaccgggt cgaagccgta cagggcaacg agcggcatga
      781 agaagggcag gcagatgagc atcatgctga cctggtcgac gaagcagccg agaagcagca
      841 gcaccagcat catggcgatc agcacggtcc acgagccgaa gccggcgttg cgcaccgctt
      901 ccaccatgcc gttggtcgcg ccggagaacg tgaggatctg ggcgaaggtc gtggcgccga
      961 tgatgatgaa aaggatgatt gccgaaatgc tcgccgtgcc catcagggct ttcatcaggt
     1021 tggacagcgt gagcgcgcgg tagatccacg ccaccgccac ggtagccact gcgccgatcg
     1081 cggcagcctc ggtgggcgtc gcccagcccg ccgccatcgc ggccaccacg acgacgaaga
     1141 tcagcaccag cggcatgacg tagagcacga gcgggttcag ccgctcccag agcgccatct
     1201 cctcgtgctc gaaggagggc gccagcgacg gatcggccag cgcgcgcagc acgatccagc
     1261 cgacgaacat gagcgagagc agcacgcccg gcacgatgcc cgcgatcagg agctgcgaga
     1321 tcgagattcc cgccaggctg ccgagcagca ccgtgagcgc cgagggcggg atcagcatgt
     1381 cgacgccgcc gatcgccatg atcggcccca tcgccatgct cgggtgatag ccgcgcctca
     1441 gcatttgcgg cagcagcagg ctgccgagga gcgccgtggt cgcgatcgtc gagccggaaa
     1501 tggcggaaaa gaccgttccc gccaccaccg ccaccaccga gagccgcccc ggcacccgcc
     1561 agatcacccg gtcgatggcg tcgatcgcct tcatcgccac gccagtgtgg aacagcacct
     1621 cccccatcag gatgaagaac gggatcggcg tgagcgagaa gctcgtgatc gagacgattc
     1681 cgttccggat gagctgcacc agcccggctt ccttcccgag gtagaaccag gcgccggcga
     1741 tattgatgcc gaagaacgcg aacgccgccg gcagccccac caggaagagc gccgtcaaca
     1801 caccaaaaag aacgatcgcg gagagccacc atgtcatgcc ccgaggttca ttgcctgagg
     1861 cgccgcgcga actccacggc gaggagggag aagcacaccg gcacgagcgc gtactgccac
     1921 cactccggga tggcgagcgt cttcaccacc atcgagccga tgcgcgacga gtcgaggatg
     1981 agccggatgc cgtaccagat gaacaccgcg cagatcgcca cgccgatcac gtcacacgct
     2041 ttctcgaggg cccgggcagc accggggggc agggacacca gcagggcatc caggcggacg
     2101 tgctcgttac gatgcaagag ccagggcgcc atgagcagcg tcgccaccgg caggctgtac
     2161 tcggacacct cgttgaccca cgcgagcgtg cccaggccga cgttgcgcat gacgacatcc
     2221 agggtgacga gcagcgccat ggcgccgagc agcaccgccg cgaccgcggc gaggcctctc
     2281 agcagcgaca cataaagctt cacttgctga agagcttcct catctgcggg ccgtactgcg
     2341 ggcttgcctt gatggcgttt tcccaggcca cgtcataggc cttggcgtag tactgcttcg
     2401 ccgttgcggc gtcgaagctg atcacctgga tgccggcttc cttttgcttg cggatatctt
     2461 cctcgttcac cttcttccac gaatcgttgg cattctcgag cgcaatcacg tgcttcatca
     2521 gaagctcgcg ctgcgcgccc gacagcgact tccacttgtc caggttcatg acgagcgaca
     2581 cttccgcgtt gtagaagccc gggtcgacgc ggaacttggt cttctcgtgc cagttgaagt
     2641 cgaagatgcc gttgatcggc cagccgtagc cgtccacgac cccgcgctcg agcgcggtgt
     2701 agacctcccc cggggcggtc tgcatcaccg tggcgccgag cgcctggaag aaatcgcggt
     2761 acaccggcgt gatgcggatc ttgaggccgg tgagatcggg cttgtcgatc ttcttcgtga
     2821 gatagaggtg gaagggcgtg tagtcgatga cccggccgag atactgcatg ttcgccttct
     2881 cgttccagat cttgttgatg aggtcgtagc cgccgttctt gcgcagctcc tgggccgaga
     2941 cctgcgtcag cttcagcgcg tcggcctcgg gcatgatgtt ggtgtagaaa gcacccgtag
     3001 tcagggccat gtccactacc cccgtgcgca ccgcgttgcc gacctcgaac ggcggcatcg
     3061 ccttcggccc gccgatgaag ttgagctgca gccgtccctt gccctcggcg ttcagcgcct
     3121 ggatcaccgg catcaggttc ttcacgtact gcgtgttctc cgcgaagccg ctcaccacgc
     3181 gcagcgtcgt ctcctgtgcc tgcgcagcaa gcggcgcggc cgccagcaac agcagcatca
     3241 gcgtcttttt catgacctcc tcctatgaag aacgacgagc gtaggcgtcc gcgattacat
     3301 ttgtcaaatc aatgtcattc atgatatgga tcacagccat gaatgtgcag gacatcgacc
     3361 tcaacctcct ccgcgtgttt gatgcggtgc tccacgagaa aggcgtgacg ccggcggccg
     3421 cacgcctggg gctcacgcag ccggcggttt cgaacgcgct cgcgcgcctg cgcaaggcct
     3481 ttggcgacgc gctgttcgtc cgaacgccgc gcggcgtgga cgccacacct ttcgcacgcg
     3541 agctcgccga gccggtgcgc caggcgctcg cgctgctcga atccgcgctc gcgcacgggc
     3601 cgggtttcga tcccgccacc tcgacccgcg cgttccgctt ctacatgtcg gatctcggac
     3661 aggtggagtt cctgccgccg ctcgtcgagc gcgtgcagcg cgtcgccgcc ggcgttcgcc
     3721 tcgaggcggt ggcgctcgat ctcgagcaca tcggcgacgc cctcgcggcg ggcgggctcg
     3781 atctcgccgt cggtttcctg cctgggctcg ggccgccgct caggcgccgc gcgctcttcc
     3841 gcgacccgta cgtctgcctg gtgcgcgccg accatccgct ggtcgccaag gggctgacgc
     3901 ggcggcgctt cctcgaagcc tcgcacgcgc tcgtcaccta ccgcggcggc ggccaccgcg
     3961 tgatcgaaga ggcgctcgag cgcgccggca tcgcccggcg tattgcgctg cgcgtcccgc
     4021 acttcacggt ggtgccgatg gtgctcgagc gcaccgacct cgtccttacg ctgccggcgc
     4081 gcgtcgcgcg cgtctacgag cggcgcggca acgtgcgctc gcttacgccg ccggtgtcca
     4141 tcccgcaggc ggaggtagcg gtgcactggc acgagcgctt cgacggcgac ccgggcaacc
     4201 gctggctgcg ggagcaggtc atcgagctgt tctcaggctg acaacgtcgc ctcaacgcag
     4261 cgcgtcggag gccgcgcgca gctccttgac cggctcgggt gcgcgtttcg cgagcgcgtc
     4321 ccagtaggcc tcgtaagcga gcttgcggtg gccggggccg aggtccacga cctggatccc
     4381 cgcgtccttg agtcgcttgt cgtcggccgc ccggcgctcc gccacccagc gcacgttctc
     4441 ggtctccagc cagtcggcca tgcggttgag gaaggcccgc tggtccccgc gcagcgactt
     4501 ccacttgtcg tggtttacga gcacgttcac caccacgtta taaaagctcg gatcgatgcg
     4561 gaacttggtg tgcgctcccc agcccatgtc gaacatgccg atgcccggcc agccatagcc
     4621 gtcgacgacg ccgcgctcga gcgcggtgag cacctcgccg ggcgccgtct gcacctgcgt
     4681 cgcgccgaga gcggcgaaga gcggctggta gttcggagtg gtgcgcagcc gcatggtctt
     4741 gaacgagccg gcgttgggca ccggcttgtt ggtccagata tgaaagccga tgccgtcgcc
     4801 atacgcagtg aggagatgcg cgttcacctt ctggacggtg tacttgtcga gggcggtcca
     4861 gaacgccgtt ttcttcagct tgggcgtctg cagcggcgcg aggatctgcg cgtcgcactc
     4921 ggggcagaag ttggtgtaga acgtcggcgc caccgacgcc atgtcgacga cgccggtctt
     4981 caccgcgttg ccttgctcga aggtcgggat cgcgtccggg ccgccgatca gcttcacctg
     5041 caccaggccc ttgccctcgc ggttcaggtg ctcgacgaag cgcacgaaga t
//
LOCUS       DASUNT010000285         2593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1002933, whole genome shotgun sequence.
ACCESSION   DASUNT010000285 DASUNT010000000
VERSION     DASUNT010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002933"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFX94_05730"
     CDS             <1..319
                     /locus_tag="VFX94_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5767717.1"
                     /translation="VVYANYVIAAALAGLGGAFAALAVGHVDPETVYWTTSGEFVFIA
                     ILAGTGNVLAPFLSAAIFEALRILASQYAPNVWQMALGAAMLAIIMFLPGGLWTLLKR
                     KPA"
     gene            316..1044
                     /locus_tag="VFX94_05735"
     CDS             316..1044
                     /locus_tag="VFX94_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5767718.1"
                     /translation="MSALLSAEALNKRFGAVTAASDITLEFDRDSVVSLIGANGAGKT
                     TFLNMVTGYLRPDSGRIRFDGQDLVGRSPREITGMGLSRSFQIPQLFATLSVRENLFV
                     AEGIAGAERAEQATEEVLARFHLEPYAARPAGLLPEGIRKLLDVAMALVARPKLLLLD
                     EPTSGVAAEEKFGIMDLVMGAVRGQGVTVLFVEHDMDIVSRYAGRVVAFYDGRIIADG
                     SPQAVLRSDEVRRYVIGEDVRAAA"
     gene            1031..1681
                     /locus_tag="VFX94_05740"
     CDS             1031..1681
                     /locus_tag="VFX94_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5767719.1"
                     /translation="MLRLENVSVSIGPVAILRGVSLGVGVGEFAGLIGRNGAGKTTLL
                     RTVMGLLPVAGGSLSYGERPLAELPTHRRISLGVGYMPEDRRLVPALSVEENVLLPAW
                     AARFADTASRLQRVYDAIPEVFELRARKALHLSGGQQKLVALARAMMAGSRLLLLDEP
                     FEGVAPVLARRLAQVIAGLRRTGVSAILSESSLAHAHGLLDRVFTIDRGAVATLSR"
     gene            1705..>2593
                     /locus_tag="VFX94_05745"
     CDS             1705..>2593
                     /locus_tag="VFX94_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX5767720.1"
                     /translation="MIRTERHFGERRVRCFVDRPATVPEVFARACAARPDAEALVCGE
                     ERLTYARLEERSVRVATALAARDIRAQDRVGVMLGNRVEFATTLLGILRLGAIAVPIN
                     ARLQRPEVAYILGQCRARALVHESNLASRVPPRSEAPTVEHILDERHVEGDGEAPALP
                     AIKEEDTAIILYTSGTTGRPKGAMLTHFNIVHSLLHYKYCFALRAGDRAVLAVPASHV
                     TGVVILLAIWHAQGCAVIMPEFKARDFLELAARERITYTILVPAMYNLCLLQPEFERF
                     DLSSWRVGGYGGAPMPEATI"
BASE COUNT          380 a          879 c          886 g          448 t
ORIGIN      
        1 cgtggtctac gccaactatg tgatcgcagc cgccctcgcg ggcctgggcg gcgcgttcgc
       61 cgcgctggcc gtcggccatg tcgatccgga aacggtgtac tggaccacct ccggcgagtt
      121 cgtgttcatc gccatccttg ccggaacggg caacgtgctc gccccgttcc tgtcggcggc
      181 gatctttgag gcgctgcgca tccttgcgag tcagtatgcc cccaatgtgt ggcagatggc
      241 gctcggagcg gcgatgctcg cgatcatcat gttcctcccc ggaggactgt ggacgctgct
      301 gaaaagaaag ccggcgtgag cgcgctcctt tccgccgagg cactcaacaa acggttcggc
      361 gccgtgaccg cggcgtccga catcacgctc gaattcgacc gcgacagcgt cgtcagcctg
      421 atcggcgcga acggcgccgg caagaccacg ttcctgaaca tggtcaccgg ttatctgcgg
      481 ccggacagcg ggcgcatccg tttcgacggc caggaccttg tcggccgctc gccgcgggag
      541 atcaccggca tggggctgtc gcgctcattc cagatccctc agctcttcgc cacgctcagc
      601 gtgcgcgaga acctgttcgt cgccgagggc atcgcgggcg ccgagcgcgc cgagcaggcg
      661 acggaggagg tgctggcgcg cttccacctc gagccttatg ccgcccggcc ggccggcctc
      721 ctgcccgaag gcatccgcaa gctgctcgac gtggcgatgg cgctcgtcgc gcggcccaag
      781 ctcctgctgc tcgatgagcc caccagcgga gtggccgccg aggagaaatt cggcatcatg
      841 gacctcgtca tgggcgcggt gcgcggccag ggcgtaaccg tgcttttcgt tgagcacgat
      901 atggacatcg tctcgcgcta cgccggccgc gttgtcgcgt tctacgatgg acgcatcatt
      961 gccgatgggt cgccgcaggc agtgctgcgc tccgatgagg tgcgccgcta cgtgatcgga
     1021 gaggacgtac gtgctgcggc ttgagaatgt ctcggtgtcg atcggaccgg tggccatcct
     1081 gcgcggggtc agcctcggcg tcggggtcgg tgagttcgcc ggcctgatcg gccgcaacgg
     1141 agccgggaag accacgctgc tacgcaccgt catggggctc ctgcccgtgg ccggcggttc
     1201 gctcagctac ggggagagac cgctcgccga gctgccgacc caccggcgca tctcccttgg
     1261 cgtggggtac atgcccgaag accggcggct cgtgccggcc ctgagcgtcg aggagaatgt
     1321 gctactgccg gcctgggccg cgcggttcgc tgataccgcg agccgtctgc aacgcgtcta
     1381 tgacgcaatc ccggaggtgt tcgagttgcg cgcgcgcaag gcgctgcatc tctcgggcgg
     1441 ccagcagaag ctcgttgcgc tggcgcgcgc gatgatggcg ggcagccggc ttcttcttct
     1501 cgacgagccg ttcgagggcg tcgcaccggt gcttgcgcgg cggctcgccc aggtgatcgc
     1561 aggactgcgg cggaccgggg tgagcgccat cctctccgaa tcgagcctgg cgcacgccca
     1621 cggcctgctc gaccgcgtgt tcaccatcga ccgcggcgcc gtcgcgacat tgtcgcgatg
     1681 acctgggcgc ttcccgcgtc ggtgatgatt cgaaccgagc ggcacttcgg tgagcgtcgg
     1741 gtacgctgct tcgtggatcg ccccgccact gttccggaag tattcgcccg cgcctgtgcg
     1801 gcacggcccg acgcagaagc gctggtttgc ggagaggagc gtttgactta cgcccggctt
     1861 gaggagcgtt ccgtgcgcgt ggccaccgcg ctcgccgcac gcgatatcag ggcgcaggat
     1921 cgcgtcgggg tgatgctggg gaaccgggtg gagttcgcca ccacgctgct cggcatcctg
     1981 cgcctcggtg ccatcgcggt gccgatcaac gcgcgactgc agcggccgga ggtggcctac
     2041 atcctcggac agtgccgggc gcgcgccctc gtgcacgaat cgaatctcgc ttccagggtg
     2101 ccgccccgga gcgaggcgcc cacggtggag cacatcctcg atgagcggca cgtcgaaggc
     2161 gacggcgagg cgccggcgct gccggccatc aaagaagaag acacggccat cattctgtac
     2221 acgtccggca ccacggggcg gccgaaaggc gcaatgctga cgcacttcaa catcgtgcac
     2281 tcgctattgc attacaagta ctgctttgcc ctccgcgccg gggatcgagc cgtgcttgcc
     2341 gtgccggcga gccatgtcac cggcgtggtg atcctgctcg ccatctggca tgcccaaggc
     2401 tgcgcggtca tcatgcccga gttcaaggcg cgcgacttcc tcgagctggc agcccgcgag
     2461 cgcatcacct acacgatcct cgtgcccgcg atgtacaacc tgtgcctgct gcagccggag
     2521 ttcgaacgct tcgatctttc gagctggcgc gtcggcggct acggcggcgc gcccatgccc
     2581 gaggcgacca tcg
//
LOCUS       DASUNT010000286         2040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_564714, whole genome shotgun sequence.
ACCESSION   DASUNT010000286 DASUNT010000000
VERSION     DASUNT010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564714"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1109)
                     /locus_tag="VFX94_05750"
     CDS             complement(147..1109)
                     /locus_tag="VFX94_05750"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5767721.1"
                     /translation="MTTFRLVLFLAAVFSSAIHAQPYPSKPIRLVVGFAPGGNTDTVA
                     RVVGQKLGERLGQQVVIENKAGAGGTIATGEVARSAPDGYTLTMGTTTTHAIAVAAYS
                     KLTYDPSADFEPLAQVAVAPYLLTVRSDFPANNLKQFVDTVKKQPGKFNYGSAGQATT
                     THLVMASFAHRAGLDVVHVPFKGNAPATTAILGGQIEILFGAIPPLLPHIKAGSLRPL
                     AISSAKRSLSLPEVPTVAESGYPGFDISLWLGFFAPKGTPAPVTQRLESDLIQIANSA
                     DVKEIMDKQGLEASAGNSGELAKLVKSEIEVYKTVFKAANIKME"
     gene            1140..1976
                     /locus_tag="VFX94_05755"
     CDS             1140..1976
                     /locus_tag="VFX94_05755"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX5767722.1"
                     /translation="MRIAFIGFGEVAAAFSAALAARGAQVSAYDVLPAKREAAERLGV
                     SFAALSEALQGADYVLSTVTTSVARHAAQQCAPHLRAGQTYVDLNATEPALKRELGRI
                     IDACGAQFVEGAILGAVGVSGAKTEILLGGPAARRAERDLAGRLGLNARFYSEEIGKA
                     SMFKMLRSVFSKGMEALLIEFLVAGERAGIREDLWKEVTGLFAAQPFEKVAANWVRSH
                     ASAYERRYHEVRQVAGVLRGLGIEPTVTAATEAFFARSRGLGLRPAADMDEVVRQFNQ
                     SL"
BASE COUNT          339 a          652 c          690 g          359 t
ORIGIN      
        1 gttgaaggag caattcgccg agcgctgccc tgtcctgatc gaggtcgcga agccctacga
       61 ccccgacgcg ctgctgaaac gcgtcctgtc gatcactccg ctgcccgatt agtacccgtc
      121 gcccacgtcg atgacgaccc gatgcatcac tccatcttga tgttggcggc cttgaacacg
      181 gtcttgtaga cctcgatctc cgatttcacc agcttcgcaa gctcccccga gttcccggcc
      241 gaggcctcca ggccctgctt atccatgatc tcctttacgt ctgccgagtt cgcgatctgg
      301 atgagatcgc tttccaggcg ctgcgtcacc ggcgcaggcg tgcccttggg cgcgaagaag
      361 ccgagccaga gcgagatgtc gaagccgggg tagcctgatt ccgccacggt cggcacctcg
      421 ggcagcgaca gcgagcgctt ggcggagctg atcgcgagcg ggcgcagcga gcccgccttg
      481 atgtggggaa ggagcggcgg tatggcgccg aacagaatct cgatctgccc gccgaggatg
      541 gccgtagtgg cgggcgcgtt gcccttgaac ggcacatgga ccacgtccag gccggcgcgg
      601 tgcgcgaagc ttgccatgac gagatgcgta gtggtcgcct ggccggccga tccgtagttg
      661 aacttgcccg gttgcttttt caccgtatcg acgaattgct tcagattgtt cgccgggaaa
      721 tccgagcgca cggtgaggag atagggcgcg accgcgacct gcgccagcgg ctcgaaatcg
      781 gcgcttgggt cgtaggtgag cttcgagtac gcagcgaccg cgatcgcgtg cgtggtggtc
      841 gtccccatgg tcagcgtgta gccgtcgggc gccgagcgtg ccacctcgcc ggtggcgatc
      901 gtgccgccgg cgccggcctt gttttcgatc accacctgct ggccgaggcg ctcgccgagc
      961 ttctgcccga ccacgcgcgc cacggtgtcg gtattgccgc cgggcgcgaa gccgaccacg
     1021 aggcggatcg gtttgctcgg atagggctgg gcatgaattg cagaggagaa aactgctgcg
     1081 aggaacaaca caagtcgaaa cgtggtcatg gggtcctccg cgcgtttaac attagtctaa
     1141 tgcgcatcgc tttcatcggg ttcggcgaag ttgcggccgc attctccgcc gcgctcgctg
     1201 cccgcggcgc gcaggtcagc gcctacgacg tgcttccggc gaagcgcgag gcggccgagc
     1261 ggctgggagt gtccttcgcc gctctgtccg aagccttaca gggcgcggat tacgtccttt
     1321 ccaccgtaac gacgagcgtg gcgcgccatg ccgcgcagca atgcgcaccc cacctgcgcg
     1381 cgggccagac ctacgtcgac ctgaacgcca ccgagccggc gctcaagcgg gagctgggtc
     1441 gcatcatcga tgcctgcggc gcgcaattcg tcgaaggcgc gatcctgggc gccgtcggcg
     1501 tgagcggcgc gaaaacggaa atcctgctcg gcgggccggc cgcgcggcgc gccgagcgcg
     1561 atcttgccgg ccggctcggc ctgaacgccc gcttctacag cgaggaaatc ggcaaggcct
     1621 cgatgttcaa gatgctgcgc agcgttttct ccaagggcat ggaggcgctt ctcatcgagt
     1681 ttctcgtcgc cggcgagcgc gcgggcatcc gcgaagattt gtggaaggaa gtgaccgggc
     1741 ttttcgcagc ccagccgttc gaaaaagtgg ccgccaactg ggtgcgcagc catgcttcgg
     1801 cctacgagcg gcgctaccac gaggtgaggc aggtggcggg cgtgctgcgc ggcctgggca
     1861 tcgagcccac ggtcaccgcg gcgaccgaag cgttctttgc acgctcgcgc ggcctcggtc
     1921 tcaggcccgc agcggacatg gacgaggtcg ttcgccagtt caaccaaagc ctatgaaaaa
     1981 gaaccgcatg aaggaaaagc tgcgcgccgg cgagccggtg ttcggcgtgt cggtgatgat
//
LOCUS       DASUNT010000287         2078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1267461, whole genome shotgun sequence.
ACCESSION   DASUNT010000287 DASUNT010000000
VERSION     DASUNT010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267461"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VFX94_05760"
     CDS             <1..582
                     /locus_tag="VFX94_05760"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5767723.1"
                     /translation="IAIARERIASLAYNDALTGLANRTSLAPALEQAIERTRRNRSKL
                     AGVFMDLDGFKQVNDMHGHAAGDAFLVEVARRLRRGLRASDIVARLGGDEFFVVLEQM
                     QDAPAVERVICKLAAEVLKPYELIGGVNARCSASLGVSIFPDDAGDAATLMAHADKAM
                     YAAKRAGKNAFCIFTADTAGNPTSIPSSGHQAA"
     gene            complement(551..>2078)
                     /locus_tag="VFX94_05765"
     CDS             complement(551..>2078)
                     /locus_tag="VFX94_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX5767724.1"
                     /translation="WIRIEPSAGRARVEFNGVRIAESARALVLHETRLPSAYYFPVED
                     VRMDLLARTAHTTHCPFKGNASYWSVKVGDEVAENAVWAYEEPYRDAEPLRGHVSFYR
                     DKVSVLQEGEQAPPAEPLHGNALAGWLVNEGWKAGTSAELAEQFLKALRQAGVPVDRS
                     TLIMPTLHPQIFATVLVWRADTPGIRTVFEPHDILHQPKFADSPFAPIIRGAGGVRRR
                     IEDPDCALDFPVVRDLKTEGATDYVAMPFRFSDGQVNVMSMTSFAPGGFSTAHLGQLY
                     EVLALLGRLFEVHAMRRTAAALLETYLGRHTGERVLKGLVKHGDGEHIHAVIWFCDFR
                     DSTPLSKSMGRQAYLRQLNRFFYCMAGAVLEAGGEVLRYIGDAVLAIFPIEGNPAGSC
                     ERAVRAARLAASRVAEVNARHQERAPLRYGIGLHLGTVTYGNIGVPQRLEFTVIGAAA
                     NEAARVEAMTKDLKQPVLTSAAFAAAYQGKLVSLGKHSLKGLEGEHELFTLPDDPMTE
                     "
BASE COUNT          331 a          724 c          693 g          330 t
ORIGIN      
        1 atcgccatcg cgcgcgagcg catcgcctcg ctcgcctaca acgacgcgct caccggcctt
       61 gccaaccgta ccagcctcgc cccggcgctc gagcaggcga tcgagcgcac gcggcgcaac
      121 cgctccaagc tcgccggcgt attcatggac ctcgacggct tcaagcaggt gaacgacatg
      181 cacggccacg cggcgggaga tgccttcctc gtggaggtgg cgcggcgcct gcgccgcggc
      241 ctgcgcgcga gcgacatcgt ggcgcgcctt ggcggcgacg aattcttcgt cgtgctggag
      301 cagatgcagg acgcacccgc ggtcgagcgc gtgatctgca agctcgcggc cgaggtgctc
      361 aagccctacg agctgatcgg cggcgtcaac gcccgctgct cggcgagcct cggcgtcagc
      421 atcttcccag acgatgcggg cgacgccgcc acgctcatgg cgcacgccga caaggcgatg
      481 tacgcggcga agcgcgccgg caagaacgcc ttctgcatct tcaccgccga cacggcaggc
      541 aatccgacgt ctattccgtc atcgggtcat caggcagcgt aaagagctcg tgctcgccct
      601 cgaggccttt cagggaatgc tttccgagag aaacaagctt cccctggtaa gcagccgcaa
      661 acgcggctga cgtcagcacc ggctgcttga ggtccttggt cattgcctcg acgcgcgccg
      721 cctcgttcgc cgcggcgccg atcacggtga actccagccg ctgcggcacg ccgatgttgc
      781 cgtaggtcac ggtgccgaga tgcagcccga tgccgtagcg cagcggcgca cgttcctggt
      841 gtcttgcatt gacttcggcg acccttgacg cggccagcct cgctgcccgc actgctcgct
      901 cgcaggaccc tgcgggattt ccctcgatcg ggaaaatcgc cagcaccgca tcgccgatgt
      961 agcgcagtac ttctccgccc gcctcgagca ccgcgcccgc catgcagtag aagaagcggt
     1021 tgagctgccg caggtacgcc tgccgtccca tgctcttcga gagcggcgtg gagtcgcgga
     1081 aatcgcagaa ccagatcacg gcgtggatgt gctcgccgtc gccgtgcttc accagccctt
     1141 tcagcacgcg ctcgccggtg tggcggccga ggtaggtctc gagcagcgcc gccgcggtgc
     1201 ggcgcatcgc gtgcacctcg aaaagccgcc cgagcagcgc gagcacctcg tagagctggc
     1261 cgaggtgcgc ggtggagaag ccgccggggg cgaacgaggt catcgacatg acgttgacct
     1321 ggccgtcgga aaagcggaac ggcatcgcga cgtagtcggt cgcgccttcg gttttcaggt
     1381 cgcgtaccac cgggaagtcg agcgcgcagt ccgggtcctc gatcctgcgg cgcaccccgc
     1441 ccgcgccgcg gatgatcggc gcgaacgggc tgtcggcgaa cttcggctgg tgcaggatgt
     1501 cgtgcggctc gaacacggtg cggatgcccg gcgtgtcggc gcgccagacg agcaccgtgg
     1561 cgaagatctg cgggtgcagc gtcggcatga tgagcgtact gcgatcgacc ggcacgcctg
     1621 cctggcgcaa cgccttcaga aactgctcgg cgagctcggc gctcgtgccc gctttccagc
     1681 cttcgttgac cagccagccc gcgagcgcat tgccgtgcag cggctcggcc ggcggcgctt
     1741 gctcgccttc ctgaaggact gaaaccttgt cgcggtagaa cgagacatgg ccgcggagcg
     1801 gctcggcatc gcggtagggc tcctcgtaag cccagaccgc gttctcggcc acctcgtcgc
     1861 cgaccttcac gctccagtag ctcgcgttgc ccttgaacgg gcaatgcgtg gtgtgcgcgg
     1921 ttctcgcgag gagatccatg cgcacatcct cgacgggaaa gtagtacgcg ctcggcagcc
     1981 gcgtctcgtg cagcacgagc gcgcgcgccg actcggcgat gcgcacgccg ttgaactcga
     2041 cgcgggcacg gcccgcgctc ggctcgatgc ggatccag
//
LOCUS       DASUNT010000288         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1003141, whole genome shotgun sequence.
ACCESSION   DASUNT010000288 DASUNT010000000
VERSION     DASUNT010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003141"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2380)
                     /locus_tag="VFX94_05770"
     CDS             complement(<1..>2380)
                     /locus_tag="VFX94_05770"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX5767725.1"
                     /translation="RIRLHYLAGRREDRLVFDLQTALAKQLGYEDTPARLASEAMMQR
                     YYHTAKAVTQLNTILLQNLEARLAPRPDTAPRPRVLLASHARLIDRAIKTLWGETGVP
                     KGAALVATGGYGRGELYPCSDIDLLVLLAAEPSDSEREALERLIGRFWDIGLEIGHSV
                     RTVEGCVEAAAGDITIRTTLLESRFLVGSRALFRRLEQALVFDPVAFFKAKKLEQEQR
                     HAKHQDSPYSLEPNLKEAPGGLRDLQVIQWIAGASGMGRRWTDLVAHKLIQRDEARQL
                     SRLERHLHDLRIRLHYLAGRREDRLVFDLQTALAKQLGYEDTPARLASEAMMQRYYRT
                     AQGVTQLNTILLQNLEARLAPRPDTAPRPLNERFEVRSELLHIRQEGTFARTPSAILE
                     CFLLMMQHPELRGMTARTLRALWRARSLVNGEFRGDSIARLLFLHILQQPRGIVHEFR
                     RMNQYGILGRYLPEFGRIVGQMQHDLFHVYTVDQHILMVVRNLRRFTMPEFAHEFPLC
                     TELMAGFERRWLLYVAALYHDIAKGRGGDHSELGTRDVRAFARNHGLAEQDRELVEFL
                     VQRHLAMSHVAQKQDVFDPEVVRAFADLVRTERRLVALYLFTVADVRGTSPRVWNAWK
                     GKLLEDLFRATRRVLTGEPLARDAALAEKQAEAARLLRLYALSDAVKEKLWASVDTAY
                     FLRHDAQEIAWHTRNLHYRVDTDKPVVKARLAPFGEGLQVMVYTRDREALFARICGYF
                     ERAGFNIVEAKVHTTRNGYALDTFLVMGQGNGLHYRDIISLIETELAEEVQSQ"
BASE COUNT          372 a          773 c          821 g          414 t
ORIGIN      
        1 actgggactg gacttcctcg gcgagctcgg tctcgatcag gctgatgatg tcgcggtagt
       61 gaaggccgtt gccctggccc atgacgagga aggtgtcgag ggcgtagccg ttgcgggtgg
      121 tgtgaacctt ggcttcgacg atgttgaagc cggcgcgctc gaagtagccg cagatgcgcg
      181 cgaacagggc ctcgcggtcg cgcgtgtaga ccatgacctg caatccttcg ccgaagggcg
      241 cgaggcgcgc cttcaccacg ggcttgtcag tatcgacccg gtaatgcagg ttgcgcgtgt
      301 gccaggcgat ctcttgcgca tcgtggcgca ggaagtacgc cgtgtcgacg ctcgcccaga
      361 gcttttcctt gacggcgtcc gaaagcgcat agagccgcaa gagccgcgcg gcctcggcct
      421 gcttctcggc aagcgcggca tcgcgcgcaa gcggctcgcc ggtgagcacg cgccgggtcg
      481 cgcggaacag atcctcgaga agcttgcctt tccacgcgtt ccagactctg gggctcgtgc
      541 cgcgcacgtc ggccacggtg aagagataca gagccacgag ccgccgctcg gtgcgcacca
      601 gatcggcgaa cgcccgcacc acttccgggt cgaagacgtc ctgcttttgg gccacatgcg
      661 acatggcgag gtggcgctgg acgagaaact cgacgagttc gcgatcctgt tccgccagcc
      721 cgtggtttct tgcgaaagcc cgcacgtctc tcgtgccaag ctcggaatgg tcgccgccgc
      781 gacccttggc gatgtcgtgg tagagcgccg cgacatagag gagccagcgg cgctcgaatc
      841 ccgccatgag ctcggtgcac agagggaact cgtgcgcgaa ctcgggcatg gtgaagcgcc
      901 gcaggttgcg caccaccatc aggatgtgct gatccaccgt gtagacgtgg aagagatcgt
      961 gctgcatctg ccctacgata cggccgaact cgggcaggta gcgcccgagg atgccgtact
     1021 ggttcatgcg gcgaaactcg tgcacgatcc cgcgcggctg ctgcaggatg tgcaggaaca
     1081 atagtcttgc gattgaatcc cctctgaatt ctccattgac cagactgcgt gcccgccaca
     1141 gcgcgcgcag cgtccgcgcc gtcattccgc gcagctcggg gtgctgcatc atgagcagga
     1201 agcactcgag aatcgcgctt ggcgtgcgcg cgaacgtccc ttcctgcctg atgtggagaa
     1261 gctcgcttct tacctcgaag cgctcgttca gcgggcgcgg cgcggtgtcg ggccgcggcg
     1321 caagccgcgc ctcgaggttc tgcaggagga tcgtgttgag ctgcgtgacg ccctgcgcag
     1381 tccggtagta gcgctgcatc atcgcttcgc ttgcgagccg cgccggcgtg tcctcgtacc
     1441 cgagctgctt tgcgagcgcc gtctgcaggt cgaacacgag gcggtcctcg cgccggccgg
     1501 cgaggtaatg caggcggatc ctgagatcgt gcaggtggcg ctcgaggcgc gaaagctgcc
     1561 gcgcctcgtc gcgctggatc agcttgtgcg ccacgaggtc cgtccagcgg cggcccatcc
     1621 cgctcgctcc ggcgatccac tggatgacct ggagatcgcg cagtcccccg ggtgcctcct
     1681 tcaggttggg ctcgagggaa tagggcgagt cctggtgctt ggcatggcgt tgctcctgct
     1741 cgagcttctt cgccttgaag aacgcgaccg ggtcgaacac aagcgcctgc tcgagccggc
     1801 ggaaaagcgc gcgactgccg accaagaacc gcgactcgag cagcgtggtg cggatggtga
     1861 tgtcgcccgc cgcggcctcc acgcagccct cgaccgtgcg cacgctgtgg ccgatctcga
     1921 ggccgatgtc ccagaagcgc ccgatcaggc gctcgagcgc ctcgcgctcg ctgtcgctcg
     1981 gctcggccgc cagcagcacc aggaggtcga tgtcggagca ggggtagagc tcgccgcgtc
     2041 cgtagccgcc ggtcgcgacc agcgccgcgc ccttcggcac gccggtctcg ccccagagcg
     2101 tcttgatcgc gcggtcgatg aggcgcgcgt ggctggcgag cagcacgcgc ggccgcggcg
     2161 ccgtatcggg acgcggcgcg aggcgcgcct cgaggttctg cagcagaatc gtgttgagct
     2221 gcgtcacggc tttcgccgtg tggtagtagc gctgcatcat cgcttcgctt gcgagccgcg
     2281 ccggtgtgtc ctcgtaaccg agctgctttg cgagcgccgt ctgcaggtcg aatacgaggc
     2341 ggtcctcacg ccggccggcg aggtaatgca ggcggatcct
//
LOCUS       DASUNT010000289         2741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_127610, whole genome shotgun sequence.
ACCESSION   DASUNT010000289 DASUNT010000000
VERSION     DASUNT010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127610"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VFX94_05775"
     CDS             complement(<1..490)
                     /locus_tag="VFX94_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011658053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX5767726.1"
                     /translation="MDKIEALLREKILLLDGAMGTMIQRARLAEEDYRGARFRDWPRE
                     LRGNNDLLSLTQPRLVRDIHSQYLQAGADIIETNTFNSTAIALADYGMESLAREFNRA
                     AARLATEAAREWKARTPDQPRFVAGVLGPTNKTASISPDVNDPGFRAVTFDELVTAYG
                     DAI"
     regulatory      complement(527..604)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            651..1082
                     /locus_tag="VFX94_05780"
     CDS             651..1082
                     /locus_tag="VFX94_05780"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX5767727.1"
                     /translation="MSEFPSIVGHFPCQVHWGDCDPAGIIFYPTYFRWMDAATWALMG
                     SVGYGPKRMREQHLAMPLVAADCQFVAPAQHGDRCEVRSRITRFGGASFVVAHDIVRD
                     DGTTLAQGSEKRVWGRYASGPGTPLKGEHIPEEVRALFRAR"
     gene            complement(1057..2433)
                     /gene="pcaB"
                     /locus_tag="VFX94_05785"
     CDS             complement(1057..2433)
                     /gene="pcaB"
                     /locus_tag="VFX94_05785"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HEX5767728.1"
                     /translation="MGRGPAGQQRDGVLRFLSLELVFSDPHTAEALSDEKLLAAMARF
                     EAALARASTGLVPVEHAETIARACESACFDVARLANEARHAGTLAIPFVKELTAQVPG
                     EAARYVHFGATSQDVIDTAVVVCLKEAAHRVLVSARRLGDAAARLADRHRDTPTVART
                     LLQPATPVPFGWKAAVWLSLVASSYRQFRAAADEVCVLQFGGPAGTLAAFGSDGELVS
                     TRLAEQLGLRKPPIPWHSARDRFARFGAEAAILAGAAGKIARDVSLLMQPEVGEAFEP
                     ASRGRGGSSALPHKRNPAACVLALEASQRAPALAASLMANLVPEHERGLGQWQAQWLT
                     LRSLVCATASGVAAMAEAIERLEVRTDTMAANLQRTQGLVFSEAVSVRLAQTLGKSAA
                     HTLTEELCARAVAENKHLREVLLAEDDVTKIIPAGELEALFEAKRAFGSAKPMIDRVL
                     AIWRETAP"
     gene            complement(2382..>2741)
                     /gene="pcaG"
                     /locus_tag="VFX94_05790"
     CDS             complement(2382..>2741)
                     /gene="pcaG"
                     /locus_tag="VFX94_05790"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEX5767729.1"
                     /translation="SPEAAQAKPIEQSFRGYGRSLTDDAGAFGFRTIRPGRVPGPDSK
                     LQAPHITVTIFMRGLLKQLQTRIYFPDDPANGDDPVLKLIPTERIGTLIAKRRADGEF
                     EWDVVLQGNNETVFFDF"
BASE COUNT          460 a          891 c          915 g          475 t
ORIGIN      
        1 cgatcgcgtc gccgtacgcg gtcaccagct catcgaacgt caccgcgcgg aagccggggt
       61 cgttcacgtc gggcgaaatc gaggcggttt tgttggtggg gccgagcacg ccggcgacaa
      121 agcgaggttg atccggcgtg cgcgctttcc attcgcgcgc ggcctcggtc gcgagccgag
      181 ccgcggcgcg gttgaactcg cgcgcaagcg actccatgcc gtagtcggcg agcgcgatcg
      241 cggtggagtt gaaggtgttg gtctcgatga tgtcggcgcc ggcctgcagg tactggctgt
      301 ggatgtcgcg cacgagccgc ggctgggtaa gcgacagcag gtcgttattg ccgcgcagct
      361 cgcgcggcca atcgcggaaa cgcgcgccgc ggtagtcttc ctccgcgagc cgcgcgcgct
      421 ggatcatggt gcccatggcc ccgtcgagca ggagaatctt ctcgcgcagg agcgcttcga
      481 tcttgtccat gtctgaaagc gaaaaagccc gctcggcttg cagagaacgg gcttcgggct
      541 cgcctcttta gcggtatttg taacgcgccc gcaagctgag catcaaatcg gcgcggcgcg
      601 tgcgattctg cccatcaagt cctgatgtgt caacggctta taatcgatcg gtgagcgagt
      661 tcccgtcgat cgtgggccat tttccgtgcc aggtgcactg gggtgactgc gaccccgccg
      721 gaattatttt ctacccaacc tattttcgat ggatggacgc cgctacctgg gccttgatgg
      781 gctcggtcgg ctatgggccg aagcgcatgc gcgaacagca tttggcgatg cccctggtcg
      841 cggcggactg ccagttcgtc gcgcccgccc agcacggcga tcgctgcgaa gtgcgctcgc
      901 gaatcacgcg cttcggcggc gcttcgttcg tggtcgcgca cgacatcgtg cgcgacgatg
      961 gcacgacgct cgcgcagggc agcgaaaagc gggtctgggg ccgctatgcc agcggacccg
     1021 gcacgcccct gaaaggcgag cacatcccgg aagaggtcag ggcgctgttt cgcgccagat
     1081 agcgagtacg cggtctatca tcggcttcgc ggagccgaag gcgcgcttcg cctcgaacag
     1141 cgcttccagc tcccccgccg gaataatctt tgtaacgtcg tcctcggcga gcagaacttc
     1201 acgcaggtgc ttattctccg ctacggcgcg ggcgcagagt tcttcggtca gcgtgtgcgc
     1261 ggccgacttg ccgagcgtct gcgcaagtcg gacagacacc gcctcggaaa acaccaggcc
     1321 ttgggtccgc tgcaggtttg ccgccatagt atcggtgcgt acctccaggc gttcgatcgc
     1381 ttccgccatg gcggcaacgc cgctcgccgt agcgcacacg aggcttctta gcgtcagcca
     1441 ttgcgcctgc cactgaccga ggccgcgctc gtgctcgggc acaaggttcg ccatcaggct
     1501 agccgcaagc gcaggagcgc gctgagaagc ttccagcgcg agcacgcagg ccgccgggtt
     1561 gcgcttgtgc ggcagggcgg atgagccgcc gcgaccgcgg cttgcgggct cgaacgcctc
     1621 gccaacttca ggctgcatca gcagcgaaac atcgcgcgct attttcccgg cggcacctgc
     1681 aaggatggcg gcttcggcgc cgaagcgggc gaagcgatcg cgcgcgctgt gccaggggat
     1741 gggcggtttc ctgagcccga gctgctcagc tagtcgcgtg ctgaccagct cgccatcgct
     1801 tccaaaagct gcgagcgtcc cggcggggcc accgaactgc agaacgcaca cctcgtcggc
     1861 cgcggcgcga aactgcctat acgagctcgc caccagcgag agccacactg cagccttcca
     1921 gccgaacggg actggcgtcg cgggctgcag cagcgtgcgc gccacggtcg gcgtatcccg
     1981 gtggcgatcg gcaagccgcg ctgcggcgtc gcccagccga cgcgccgaca caagcacgcg
     2041 gtgcgccgct tccttcaggc agacgaccac cgcggtatcg attacgtcct ggctggtcgc
     2101 gccgaagtgg acatagcgcg cggcttcacc agggacctgc gcagtcagtt ccttgacgaa
     2161 cgggattgcg agcgtacccg catgcctcgc ttcattcgcg agccttgcga catcaaaaca
     2221 ggcgctctca caagcgcgtg caatggtttc agcatgctcg accggaacaa gacccgtact
     2281 cgcgcgggcg agcgccgctt caaagcgcgc catagccgcg agcagcttct cgtccgagag
     2341 cgcttcggcg gtatgcgggt ccgaaaagac gagctcgagg ctcagaaatc gaagaacacc
     2401 gtctcgttgt tgccctgcag gaccacgtcc cactcgaact cgccgtcggc gcgcctcttc
     2461 gcgatcagcg tgccgatgcg ctcagtgggg atgagcttca gcaccgggtc gtcgccgttg
     2521 gccggatcgt cgggaaaata gatacgggtt tgaagctgct tgagcagccc acgcatgaag
     2581 atggtgacag taatgtgcgg cgcctgcagc ttgctgtcgg ggccgggcac gcggccgggc
     2641 ctgatggtgc ggaagccgaa agcgcccgca tcgtcggtga gcgaacggcc gtagccgcga
     2701 aagctctgct ctatcggctt cgcttgcgca gcctcggggc t
//
LOCUS       DASUNT010000290         3266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1091262, whole genome shotgun sequence.
ACCESSION   DASUNT010000290 DASUNT010000000
VERSION     DASUNT010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091262"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1554
                     /gene="infB"
                     /locus_tag="VFX94_05795"
     CDS             <1..1554
                     /gene="infB"
                     /locus_tag="VFX94_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX5767730.1"
                     /translation="EAYLVETQEAGASELMPRPPVVTVMGHVDHGKTSLLDAIRHANV
                     VAGEAGGITQHIGAYQVEQNGQKITFIDTPGHAAFTAMRARGAQATDIAVLVVAADDS
                     VMPQTIESINHAKAAGVPIIVAINKIDKHEANPEKVKQELLSHGVVPEEFGGETPFVP
                     VSAKTGQGIDALLERVLLQAEVQELKAAVHVPARGVVIEARLDKGKGPVATVLVQSGT
                     LQRGDVVLAGAVHGRVRAMLDESGKPIQSAGPSIPVEIQGLSDVPLAGEEMVALADER
                     KAREIALFRQGKFRDVKLAKQHSAKLENIFETMGEGEKKTLALVIKSDVQGSQEALVH
                     ALTRLATDEVKVTVVHAGVGGITESDVNLAMASRAVIIGFNTRADATARKLIESAGIQ
                     VRYYNIIYDAVEEIKAALSGMLAPERKESVLGLVEVRQVFRISKIGTVAGCYVQEGLV
                     KRNAQVRVLRDNVVIHTGEIDSLKRFKDDVREVKAGFECGISLKNFSDLKEKDQFEVF
                     EVLEVARTL"
     gene            1551..1901
                     /gene="rbfA"
                     /locus_tag="VFX94_05800"
     CDS             1551..1901
                     /gene="rbfA"
                     /locus_tag="VFX94_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX5767731.1"
                     /translation="MRKGHGRPQKLGDLIQRELSELLQRELRDPRVGMITITGVNVSP
                     DFSHAKVFYTTLEPAHVADAARGLKRAAGFLRSQLAKRIKLYTTPELRFEYDESIERG
                     DRLARLINSVNKRD"
     gene            1894..2802
                     /gene="truB"
                     /locus_tag="VFX94_05805"
     CDS             1894..2802
                     /gene="truB"
                     /locus_tag="VFX94_05805"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX5767732.1"
                     /translation="MIDGALLLDKPVGVSSNAVLQRTKQLLGATKAGHAGTLDPLASG
                     LLLALFGEATKFAGPLLDSDKEYLATLKLGERTTTGDAEGDVVSKKDVNPENLPAVLE
                     RFRGEIEQVPPMHSALKHKGTPLYRLARRGEEVPRAARLVRISRLELVKLALPALVLR
                     VACSKGTYIRVLAEDIGAALGCGAHLSALRRTGSGPFRVEDALTLDALECAPLAERRR
                     SVLPLSELLRGLPRAELDAAGEARLRQGRALKVGGLKRGLCAVVRPDGGVIGLGAADG
                     DGGLKPLRLTQTAEKHLESAAHMKRE"
     gene            2807..3076
                     /gene="rpsO"
                     /locus_tag="VFX94_05810"
     CDS             2807..3076
                     /gene="rpsO"
                     /locus_tag="VFX94_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX5767733.1"
                     /translation="MALQVAQKQQVVKQYQRSGKDTGSPEVQIALLTERINSLTEHFK
                     THVKDFHSRRGLLKLVSQRRKLLDYLKRTDADQYRQLIGKLELRK"
BASE COUNT          617 a         1052 c         1107 g          490 t
ORIGIN      
        1 gaagcgtatc tggtcgaaac ccaggaagcc ggcgcgagcg agctgatgcc gcggccgccc
       61 gtcgtgaccg tgatgggcca cgtcgatcac ggcaagacgt cgctgctcga cgcgatccgc
      121 cacgccaatg tggtcgccgg cgaagcaggc ggcatcaccc agcacatcgg tgcctaccag
      181 gtcgaacaga acggccagaa gatcaccttc atcgacaccc ccggccacgc cgcgttcacc
      241 gcgatgcgtg cccgtggcgc gcaggcgacc gacattgcgg tgctggtcgt cgcagccgac
      301 gacagcgtga tgccgcagac gatcgaatcg atcaaccacg ccaaggcggc cggcgtgccg
      361 atcatcgtgg cgatcaacaa gatcgacaag cacgaggcga atccggagaa ggtcaagcag
      421 gagctgctca gccacggcgt cgtgccggag gagttcggcg gcgagacgcc gttcgtgccg
      481 gtctcggcga agaccggcca gggcatcgac gcactgctcg agcgagtgct gctgcaggcc
      541 gaagtccagg aattgaaagc ggcggtccac gtccccgcaa gaggcgtcgt catcgaagcc
      601 cgcctcgaca aggggaaggg cccggtcgcc accgtgctgg tccagtccgg gacgctgcag
      661 cgcggcgacg tcgtgctcgc cggagcggtg cacggacggg tgcgcgcgat gctcgacgag
      721 agtggcaagc cgattcagtc ggcgggcccg tcgatcccgg tcgagatcca gggtctgtcg
      781 gatgtcccgc ttgcgggcga ggaaatggtc gcgctcgccg atgagcgcaa ggcgcgcgag
      841 atcgcgctgt tccggcaggg caagttccgc gacgtgaagc tcgccaagca gcattcggca
      901 aagctcgaga acatcttcga gacgatgggc gaaggcgaga agaagacgct ggcgctggtg
      961 atcaagtcgg acgtgcaggg ctcgcaggag gcgctggtgc acgcgctgac ccggctcgcg
     1021 accgacgagg tgaaggtgac cgtggtacac gccggcgtgg gcggcatcac cgagtcggac
     1081 gtgaacctcg cgatggcctc acgcgcggtg atcatcggct tcaacacccg cgccgacgcc
     1141 accgcgcgca agctgatcga gtcggccggc atccaggtgc gctactacaa catcatttac
     1201 gacgcggtcg aggagatcaa ggcggcgctc tccggcatgc tggcgccgga gcgcaaggag
     1261 tcggtgctgg ggctggtgga agtgcgccag gtattccgca tctccaagat cggcaccgtc
     1321 gccggatgct acgtgcagga gggcctcgtg aagcgcaacg cgcaggtccg cgtgctgcgc
     1381 gacaacgtcg tgatccacac cggcgagatc gattcgctca agcgcttcaa ggacgacgtg
     1441 cgcgaggtga aagccggctt cgaatgcggc atctcgctca agaacttctc cgatctcaag
     1501 gagaaggacc agttcgaggt attcgaggtg ctggaggttg cgaggacgct ttgaggaaag
     1561 gacatggtcg tccgcagaag ctcggcgacc tgatccagcg cgagctctcc gagctcctgc
     1621 agcgcgagct gcgcgatccg cgcgtcggca tgatcaccat caccggggtg aacgtctccc
     1681 ccgacttctc gcatgcgaag gtgttctaca ccaccctcga gcccgcgcat gtagccgatg
     1741 cggcacgagg cctgaagcgg gcggccggat tcctgcgctc gcagcttgcg aagcgcatca
     1801 agctctacac cacgcccgag ctgcgcttcg agtacgacga gtcgatcgag cggggtgacc
     1861 ggcttgcgcg tcttatcaac agcgtgaaca agcgtgattg acggcgcgct gctgctcgac
     1921 aagcccgtgg gggtcagctc caacgcggtg ttgcagcgga cgaagcagct tctcggcgcg
     1981 accaaggccg gccacgccgg cacgctcgac ccgctcgcct cggggctgct tctggcgctc
     2041 ttcggcgagg cgacgaagtt cgccgggccg ctgctcgatt ccgacaagga atacctggcg
     2101 acgctcaaac tgggcgaacg cacgacgacc ggcgacgccg aaggcgatgt cgtctcaaag
     2161 aaagacgtaa accccgaaaa cctccctgcg gtcctcgagc gcttccgcgg cgagatcgag
     2221 caggtgccgc ccatgcattc ggcgctgaag cacaagggca cgccgcttta ccgcctcgct
     2281 cgccgtggag aggaagtgcc tcgcgccgcg cgcctggtcc gcatttcgcg cctggagctg
     2341 gtgaagttgg cgcttccggc acttgtcctg cgcgtcgcct gcagcaaggg aacctacatc
     2401 cgcgtgctcg ccgaggacat cggtgcggcg ctcggctgcg gcgcgcatct ctcggcgctg
     2461 cgccgcaccg gctcggggcc gtttcgcgtg gaggatgcgc tcacgctgga tgcgctcgaa
     2521 tgcgcgcctc tggccgagcg ccgccgcagc gtgctgccgc tatcggagct gttgcgcgga
     2581 ttgccgcgcg cggagctgga cgccgccggg gaagcgcgcc tgcggcaggg ccgggcgttg
     2641 aaggtcggtg ggctgaaacg cgggctgtgc gcggtggtgc gtccggacgg cggcgtgatc
     2701 ggtctcggcg ccgccgacgg cgacggcggc ctgaagccgc tgaggctcac ccaaacggct
     2761 gaaaaacatt tagaatccgc ggctcacatg aagagagagt agagtgatgg ctttgcaagt
     2821 agcgcagaag cagcaggtcg tgaagcagta tcaacgctcc ggcaaggaca caggctcacc
     2881 cgaagtccag atcgcgctgc ttaccgagcg catcaacagc cttaccgagc atttcaagac
     2941 tcacgtcaag gacttccact cgcgtcgcgg gctgctgaag ctggtgagcc agcggcgcaa
     3001 gctgctcgac tacctcaagc gcaccgacgc agaccagtac aggcagctga tcggcaagct
     3061 cgagctgcgt aaatagacgc aaacgaaacg cccactccgg tgggcgtttt agctggatcg
     3121 ttcttccgag ccgtccggac cgggccgctc gccaaccagg aaaccctatg ctacccactg
     3181 ccatcaagaa gacattcacc tatggcgccc accaggtaac gctggcgacc ggcgagatcg
     3241 cccgccaggc ccacggcgcc gtcatg
//
LOCUS       DASUNT010000291         4052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_215066, whole genome shotgun sequence.
ACCESSION   DASUNT010000291 DASUNT010000000
VERSION     DASUNT010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215066"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1811)
                     /gene="aceE"
                     /locus_tag="VFX94_05815"
     CDS             complement(<1..1811)
                     /gene="aceE"
                     /locus_tag="VFX94_05815"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010109940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX5767734.1"
                     /translation="MTDLADIVSSAFWRVNPPDADPAETREWLEAFDALVAAEGRERA
                     TFLLRKLLDHARARRVPLPPVLNTPYKNSIGLAHQPQFPGNLELESRISAIVRWNALA
                     MVVRANRAHPELGGHIASYASAADLFEVGFNHFFRSNDLVYFQPHSAPGVYARAYLEG
                     RLTEAELGHYRRETAGKGLSSYCHPYLMPRFWQFPTGSMGLGPINAIYQARFMRYLEH
                     RKALQTQGRKVWAFAGDGEMDEPESLAGLSAAAREGLDNLVFVVNCNLQRLDGPVRGN
                     GSIVQELEGLFAGAGWNVIKLLWGSDWDPLFARDEEGILLKRLHETVDGEFQTYAATD
                     GRFNREHFFNKYPELRELIAGMSDDDIDRLHRGGHDPVKIYAAYHAAVHHKGQPTVIL
                     AKTKKGYGMGHWGQGKMGTHQQKKLDDDALREFRDRFALPLSDEDVAQLRFYHPGPAS
                     PEIKYLLERRQELGGFVPARNTRAETLPVPALAAPERSQSTTMVFVQLLSQLLKDEKL
                     GPRVVPIVADEARTFGMQTLFRQVGIYSALGQLYEPEDHDELLYYREAKDGQILEEGI
                     TEAGSMSSFIAAGTAYASHGVPMIPIFIYYSMFGFQR"
     gene            2040..2243
                     /locus_tag="VFX94_05820"
     CDS             2040..2243
                     /locus_tag="VFX94_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX5767735.1"
                     /translation="MPTGTVKWFNDSKGFGFITPSEGGEDLFAHHTAIQMSGFKTLKE
                     GQKVEFEVKRGPKGLQAHNIKPL"
     gene            complement(2286..>4052)
                     /gene="glsA"
                     /locus_tag="VFX94_05825"
     CDS             complement(2286..>4052)
                     /gene="glsA"
                     /locus_tag="VFX94_05825"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEX5767736.1"
                     /translation="ARFLGLTDGRVADYIPELSRADPDWFGICIATRDGFIYEVGDSR
                     QPFTLQSISKPLSYGLALEENGEDKLLAKIGVEPSGDAFNAISLRPQTGAPLNPLINA
                     GAIAACGLIPGNTVEEKTARILAAFSRYAGRTLDIDERVYQSESATGHRNRAIGWMLR
                     NFSILADEPSPTLEAYFRQCSIRVTCRDLALIGATLANGGVNPVSRVRALPEQYVDNV
                     LSVMATCGMYDFSGEWLYRTGLPAKSGVGGGILAVQPGRLGIGVFSPRLDAQGNSVRG
                     IAVCRALAADLGLHLFDAAKPAAPAVRLSTTRRKVSSKQRLPALAAEHLRNAGKRLRL
                     YHLQGPLNFAAIEPVVRELIARAADTDCFILNLKMVQALDSAATRLLAGTRRELRNLG
                     KPVVFSEVGAWWQQLIDAGIDREAVFSDDDFALEYGENLLLAERFQSLAWDAETPLQK
                     CALFSGFAAEELAGLEPLLATRTYHRGQTIVAAGQSADELFVITRGLAMVSIPTQNGV
                     ARIDSFSSGMTFGDVAFIDRSPRSANVTALGPVECRVLTREAFARLDAEAPATKIRLL
                     QNLAQGMTAMVRSLSRELAALK"
BASE COUNT          717 a         1286 c         1369 g          680 t
ORIGIN      
        1 atgcgctgga agccgaacat cgagtagtaa atgaagatcg ggatcatcgg cacgccatgg
       61 ctggcatagg cggtgccggc ggcgatgaag ctcgacatgc tgccggcctc ggtgatcccc
      121 tcctcgagaa tctggccgtc cttggcctcg cggtaataaa gcagctcgtc atgatcctcg
      181 ggctcgtaga gttgcccgag cgcggagtag atgccgacct ggcggaagag cgtctgcatg
      241 ccgaaagtgc gcgcctcgtc ggcaacgatc ggcaccacgc gcggcccgag cttctcgtcc
      301 ttgaggagct gcgaaagaag ctgcacgaac accatcgtgg tggactgact gcgctcgggc
      361 gctgcgaggg ccggcacagg gagcgtttcc gctctcgtgt tcctggcggg aacaaagccg
      421 cccagctcct gccttcgctc gagcaggtac ttgatctccg ggctggccgg gcctggatgg
      481 tagaagcgaa gctgcgcgac atcctcgtcc gacagcggca gcgcaaagcg gtcgcggaac
      541 tcgcgcaggg cgtcatcgtc gagcttcttc tgctggtggg tgcccatctt gccctgcccc
      601 cagtggccca tgccgtagcc tttcttggtc ttggcgagaa tgaccgtcgg ctggcccttg
      661 tggtgcacgg cggcgtggta cgcggcgtaa atcttcaccg ggtcgtgccc gccacggtgc
      721 aggcggtcga tgtcgtcgtc gctcatgccc gcgatgagct cgcgcagctc cggatacttg
      781 ttgaagaaat gctcgcggtt gaagcgcccg tcggtcgcgg cgtaggtctg gaactcgccg
      841 tccaccgttt cgtgcagccg cttgagcagg atgccctcct cgtcgcgcgc gaagagcgga
      901 tcccagtccg agccccagag gagcttgatc acgttccagc cagccccggc gaagaggccc
      961 tcgagctcct gcacgatcga gccgttgccg cgaaccggac cgtcgaggcg ctggaggttg
     1021 cagttgacca cgaagacgag gttgtcgagc ccttcgcgcg ccgcagccga aaggccggcg
     1081 agcgattcgg gctcgtccat ctcgccgtct ccggcgaacg cccacacttt tcgcccctgc
     1141 gtctgcagcg cctttctgtg ctcgagatag cgcatgaagc gcgcctggta gatcgcattg
     1201 atgggcccga ggcccatcga gccggtcggg aattgccaga accgcggcat caggtaggga
     1261 tggcagtaag acgacaggcc cttgcccgcg gtctctctcc tgtagtggcc gagctcggcc
     1321 tcggtcaggc gaccttccaa ataagccctc gcatacaccc caggcgcgga atgcggctgg
     1381 aagtacacga ggtcattgga acggaagaag tgattgaagc cgacctcgaa gagatccgcg
     1441 gcagaggcat agctcgcgat atggccgccg agctcgggat gcgcgcggtt cgcgcgcacg
     1501 accatggcga gcgcattcca ccgcacgatc gcggagatgc ggctctccag ctccaggttt
     1561 cccgggaact gcggctgatg cgcgagcccg atcgaattct tgtatggcgt gttcaggacc
     1621 ggcgggagcg gtacgcgccg cgcgcgggca tggtccagca gcttgcgaag gaggaaggtc
     1681 gcacgctcac ggccttccgc tgcgacgagg gcgtcgaaag cttcgagcca ttcgcgggtt
     1741 tcggcgggat cggcgtccgg cgggttgacg cgccagaagg cggaggaaac gatgtcggcg
     1801 agatcggtca tccgggcatg gtaatagtcc ggtcgccccc gccgctatcc cccaatgccg
     1861 tccggaaaaa tggtgctatg atcccccgcg caggatgcgt ttcaagcagc gaaagtgccg
     1921 tcccctcctc gtcgcctcaa tcagcgcgtc ggtagtggca atcggttcca actcttgatt
     1981 cttcgtgccc ggcttcgtcg cgcccggatt cgtcgcggca attgcagagg taacaggtaa
     2041 tgccaaccgg taccgttaag tggttcaatg actccaaagg cttcggcttc atcaccccga
     2101 gcgagggcgg cgaggatctg ttcgcgcatc acaccgcgat ccagatgtcc ggcttcaaga
     2161 ccctgaagga ggggcagaag gtagagttcg aggtgaagcg cgggccgaag ggactccagg
     2221 cgcacaacat caagccgctg taagagtcgg gacggcaacc aaaagcccgc gcgagcgggc
     2281 tttcttcatt tcagggcggc aagctcgcgg ctgaggctgc gcaccatcgc ggtcatgccc
     2341 tgcgcaaggt tctgcagcag ccggatcttg gtggcgggcg cttcggcgtc gagccgagca
     2401 aaggcctcgc gggtgaggac ccggcactcc accggcccca gcgcggtgac attcgccgag
     2461 cgcggcgagc ggtcgatgaa ggcaacatcg ccgaaggtca tgccggagct gaacgagtcg
     2521 atccgggcga cgccgttctg cgtcgggata ctgaccatgg ccaagccgcg cgtaatcaca
     2581 aaaagctcgt ccgcgctctg cccggccgcg acgatcgtct gaccgcggtg ataagtccgg
     2641 gtggcgagga gcggctccag acccgcaagc tcctcggctg cgaagccgct gaacagcgcg
     2701 cacttctgga gcggcgtttc cgcatcccag gcaaggctct ggaagcgctc ggcaaggagc
     2761 aggttctcgc cgtactcgag cgcgaagtca tcgtcggaaa aaactgcctc ccgatcaatg
     2821 ccggcgtcga tgagttgctg ccaccaggcg ccgacctcgg agaacacgac gggcttgccg
     2881 agattgcgca gttccctgcg cgtgccggcg aggagccgcg tcgccgcgga gtcgagcgcc
     2941 tgcaccatct tgaggttcag gatgaagcaa tcggtgtcgg ccgcgcgcgc tatcagctcg
     3001 cgcacgaccg gctcgatcgc ggcgaagttg aggggcccct gcaggtgata gagccgaagc
     3061 cgcttgccgg cgttgcgcag gtgctcagcg gcgagcgcag ggaggcgctg cttcgaggac
     3121 actttgcgcc gcgtggtcga caggcgcacc gccggcgcgg ccggcttggc cgcgtcgaag
     3181 agatgcagcc cgagatccgc cgcgagcgcg cggcacaccg cgatgccgcg gacgctgttc
     3241 ccttgtgcat ccaggcgcgg ggaaaagacc ccgatgccga gccggccggg ctgcacggcc
     3301 agaatgccgc cgcccacccc gctcttcgcc ggcaagcccg tgcggtagag ccattcgccc
     3361 gagaagtcgt acatgccgca ggtcgccatg acggaaagca cgttgtcgac gtattgctcc
     3421 ggcagcgcgc ggacgcgcga cacggggttc acgccgccat tcgcgagcgt tgcgccgatg
     3481 agcgcaagat cgcggcacgt cacccgtatc gagcattggc ggaagtacgc ctccagcgtc
     3541 ggcgacggct cgtcggcgag gatggagaaa tttcgcagca tccagccgat cgcgcgattg
     3601 cggtggccgg tggcgctttc cgactggtag acgcgctcgt cgatgtcgag cgtgcgaccc
     3661 gcatagcggg agaaggcggc aagtatgcgg gctgtctttt cctccaccgt gtttccgggg
     3721 atcagcccgc aggccgcgat cgcaccagca ttgatcagcg gattgagcgg cgcaccagtt
     3781 tggggccgca ggctgatggc gttgaacgcg tcgcccgatg gctccacgcc gattttcgcc
     3841 agcagtttgt cctcgccgtt ttcctccagc gccagcccat aggaaagcgg cttggaaatc
     3901 gactggagcg tgaagggctg gcgcgagtcg ccgacttcgt agatgaaccc gtcgcgcgtg
     3961 gcaatgcaga tgccgaacca atccggatcg gcgcgcgaaa gctcgggaat gtagtccgcg
     4021 acccggccgt ccgtgaggcc caggaaacgg gc
//
LOCUS       DASUNT010000292         4937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_653506, whole genome shotgun sequence.
ACCESSION   DASUNT010000292 DASUNT010000000
VERSION     DASUNT010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653506"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..564)
                     /locus_tag="VFX94_05830"
     CDS             complement(25..564)
                     /locus_tag="VFX94_05830"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HEX5767737.1"
                     /translation="MDDSALLIVDIQNDYFPGGAMQLEGADAAAANAARVLSDFRGRQ
                     KPVIHVRHLSVRPGATFFLPGTRGAEIHSSVQPVSGETVIEKNFPNAFRGTGLRELLE
                     KHNTKNLVIAGMMTHMCVDATVRHAADLGYSITLLGDACATRAQSYGGDTVPARQVHA
                     AFLAALNGFYAKVIKTHDL"
     gene            complement(557..1168)
                     /locus_tag="VFX94_05835"
     CDS             complement(557..1168)
                     /locus_tag="VFX94_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HEX5767738.1"
                     /translation="MPKVYAIDGITPVVHPSAFVHPSAVLIGDVIVGARAYIGPCASL
                     RGDFGRIVVEAGANIQDTCMLHGFPGKDTVVGAESTIGHGAVLHGCVVRRGALVGMNS
                     VVNDNAEVGEDAVIAALAFVRAEESIPARSLAVGIPARVVRKLSPQELGWKKDNMRLY
                     QELAVRSAGSMELVEALTEPEANRKSIEIPGSIPLSELKKKHG"
     gene            1186..1953
                     /gene="paaG"
                     /locus_tag="VFX94_05840"
     CDS             1186..1953
                     /gene="paaG"
                     /locus_tag="VFX94_05840"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017693479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HEX5767739.1"
                     /translation="MASVLVSVEAGVQTLTLNRPEKLNAFNPEMHKELRRALEQALDE
                     RSIGCVLLTGAGRGFCSGQDLSERNVAPGAAPIDLSVSLGSHYNPLVRRMRELPKPIV
                     CAVNGVAAGAGANIALACDIVLAARSASFVQSFARLGLVPDSGGTYFLPRLVGTARAM
                     ALALLAEPLSAEKAESWGLIWKALDDERLMPEATALARTLAAGPTKGFALVKRAIYAS
                     GGNSLDAQLDLERDLQREAGYSEHYRNAVAAFIEKRK"
     gene            1950..3206
                     /gene="paaK"
                     /locus_tag="VFX94_05845"
     CDS             1950..3206
                     /gene="paaK"
                     /locus_tag="VFX94_05845"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HEX5767740.1"
                     /translation="MKAFPLEPVEKCSTQELKRLQLERLRWSLAHAYDNVARYRQKFD
                     AAGVKPGDCRSLEDLARFPFTAKADLRETYPFGMFAVPMDRIVRIHASSGTTGKPTVV
                     GYTAKDIDTWSHLMARSMRAAGARPGDKVHVAYGYGLFTGGLGAHYGAERLGLAVIPL
                     GGGMTERQVQLIVDFKPDIIMVTPSYMLAIADEMERQGVDPKKTSLRVGIFGAEPWTN
                     ELRQQIEQRVSLDAIDLYGLSEVMGPGVAQECAETKDGLTIWEDHFYPEIIDPQSGEV
                     LPEGEKGELVLTSLTKEALPVIRYRTRDLTRLLPGSARTMRRIEKITGRSDDMLIIRG
                     VNVFPSQIEELVLKQPGLAPHYVLEVTKHGPLDYLAVIVEGEAGACKALEHHIKSRIG
                     ISAEVRLGTVERSIGKAKRVIDKRPK"
     gene            complement(3208..3405)
                     /locus_tag="VFX94_05850"
     CDS             complement(3208..3405)
                     /locus_tag="VFX94_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5767741.1"
                     /translation="MTALTPRELDVLRLLASGCSYLQAAIRLGISPHTVVTHVKKTYG
                     KLEVHSAGAAVMRPVRLGVLE"
     gene            complement(3417..4574)
                     /locus_tag="VFX94_05855"
     CDS             complement(3417..4574)
                     /locus_tag="VFX94_05855"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEX5767742.1"
                     /translation="MRAVLIALLVAAVSPACADEEALQRAFVHAMQAQQAGDLAHAEA
                     IFRAMLEHTDSPRVKLELARTLFLQAKHEEAKRLFKEVSVQSDTPWRVRDNIALFVRE
                     IEERTGYLKLGVTVVSDSNPGNIARQKEFAIGGVLVTSSAAPEKVTGLRYSAQGWLPL
                     KPIGAAGYLTASYVDYPEQDFDRLTVDAGLMKNLVESGRVRGKAGLEFGTLGGNGLYR
                     FPYVGLDAVLAQSESYRLAGELKLGRVTYPDFEYLDSTYRSGALSLRRELSQTVAGTL
                     RGSVEGSHAEERPYSYYGWDIAPGISTFWPQYMFLVNASLTYGSRKYADADPLFGERR
                     DDDRVRAEVTVGNKKWRWRDSYIALVASVEETRSNIVFYSYRKTNVSVVVE"
     gene            complement(4578..>4937)
                     /locus_tag="VFX94_05860"
     CDS             complement(4578..>4937)
                     /locus_tag="VFX94_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEX5767743.1"
                     /translation="GSATFTGKLIGFYVAPTGAGATAGADVTVNANFSSRSLGFASSG
                     TTVQGSAAPQLDLRGTLTYAPGQASFSGAVVNAGGSMTGSSKGQFYGPNAEELGGAFR
                     LQGAGNELFTGGFGAKR"
BASE COUNT          819 a         1668 c         1626 g          824 t
ORIGIN      
        1 cctttctgct ctaccgtgat gaattcatag gtcatgggtc ttgatcactt ttgcgtagaa
       61 accgttgaga gcggcgagga aagcggcgtg cacctgccgc gccggtaccg tgtcgccgcc
      121 gtagctttgc gcgcgcgtgg cgcaggcgtc gcccagcaga gtgatgctgt agccgaggtc
      181 ggccgcgtgg cgcacggtcg cgtcgacaca catgtgcgtc atcatcccgg cgatgacgag
      241 atttttcgtg ttgtgctttt cgaggagttc tcttagcccg gtgccgcgaa aggcgttcgg
      301 gaaattcttc tcgatgacgg tctcgccgct caccggctgc acggaggaat ggatctccgc
      361 gccgcgcgtg cccggcagaa aaaaggtcgc cccgggacgc accgacaggt ggcgtacgtg
      421 gatgacgggc ttttgtcggc ctcgaaaatc ggacaagacg cgtgcagcgt tagccgcggc
      481 cgcatcggcg ccctcgagct gcattgcgcc gcccgggaaa tagtcgttct ggatgtcgac
      541 gatcaggagc gcgctgtcat ccatgcttct tcttcagctc ggacagcgga atggagcccg
      601 gaatttcaat cgatttgcgg tttgcttccg gttcggtcag ggcctccacg agctccatcg
      661 agcccgcgct gcgcaccgcg agctcctggt agagccgcat gttgtccttc ttccagccga
      721 gctcctgcgg cgaaagcttg cgcaccacgc gcgccgggat cccgaccgcg agcgagcgcg
      781 ccggaatgga ctcctcggcc cgcacgaagg cgagcgcggc gatcaccgcg tcctcgccca
      841 cttcggcgtt gtcgttcacg acgctattca tgccgacgag cgctccgcgg cgcacgacgc
      901 acccgtgcag cactgcgccg tggccgatgg tgctttcggc gcccaccacg gtgtccttgc
      961 cggggaagcc gtgcagcatg caggtgtcct ggatattggc gcctgcctcg accacgatgc
     1021 ggccgaagtc gccgcgcagg ctggcgcacg gcccgatgta ggcgcgcgcg cccacgatca
     1081 cgtcgccgat cagcaccgcg ctcgggtgca cgaaagcgct cgggtgcacg acgggcgtga
     1141 tgccgtcgat ggcgtacacc ttaggcatgg cagaattcta actccatggc ttcggtgctc
     1201 gtctccgtcg aagcaggcgt tcagaccctg accctgaacc ggccggagaa gctgaacgcg
     1261 ttcaatcccg agatgcacaa ggagctgcgc cgcgcgctcg agcaggcgct cgacgagcgc
     1321 tcgatcggct gcgtgctcct caccggtgcc gggcgcggct tctgctcggg acaggacctt
     1381 tcagagcgca acgtcgcgcc gggcgctgcg ccgatcgacc tctcggtttc gctcggctcg
     1441 cactacaacc cgctcgtgcg ccgcatgcgc gagctgccga agccgatcgt gtgcgcggtg
     1501 aacggggtcg ccgccggcgc cggcgcgaac atcgcgctcg cctgcgacat cgtgctcgcc
     1561 gcgcgctcgg cgagcttcgt gcagtccttc gcccggctcg gcctcgtccc cgactccggc
     1621 ggcacctatt ttctgccgcg cctcgtcggc acggcgcgcg cgatggcgct cgcgcttctg
     1681 gccgagccgc tcagcgccga gaaggccgaa agctggggac tcatctggaa ggcgctcgac
     1741 gacgagcgcc tgatgcccga ggcgaccgcg ctcgcgcgca cgctggccgc cgggccgacg
     1801 aaaggattcg cgctcgtgaa aagagcgatc tacgcctcgg gcggcaattc gctcgatgcg
     1861 cagctcgatc tcgagcgcga cctgcagcgc gaagcgggat attcagagca ttaccgcaac
     1921 gcggtcgccg cgttcatcga gaagcgcaag tgaaggcgtt cccgctcgag ccggtcgaga
     1981 aatgctcgac gcaggagctg aagcggcttc agctcgagcg gttgcgctgg tcgctcgcgc
     2041 acgcctacga caacgtggcg cgctaccgcc agaagttcga cgccgcgggc gtgaagcccg
     2101 gcgactgccg ctcgctggaa gacctggccc gcttcccctt caccgccaag gccgacctgc
     2161 gcgagacgta cccgttcggc atgttcgccg tgccgatgga tcgcatcgtg cgcatccacg
     2221 cctcctccgg cacgaccggc aagccgacgg tggtcggcta caccgcgaag gacatcgaca
     2281 cctggtcgca cctcatggcg cgctcgatgc gcgccgccgg cgcgcgaccc ggcgacaagg
     2341 tgcatgtcgc ctacggctac ggcctcttca cgggcgggct cggcgcgcat tacggcgccg
     2401 agcggctagg cctcgcggtg atccccctcg gtggcggcat gaccgagcgg caggtgcagc
     2461 tcatcgtgga cttcaagcct gacatcatca tggtcacgcc gagctacatg ctcgcgatcg
     2521 ccgacgagat ggagcggcaa ggcgtcgacc ccaagaagac ttcactgcgc gtcgggatct
     2581 tcggcgccga gccgtggacc aacgaattga ggcaacagat cgagcaacgc gtttcgctcg
     2641 acgcgatcga cctttacggg ctgtcggaag tcatgggtcc gggcgtggcg caggagtgcg
     2701 ccgagaccaa ggatggcctc acgatctggg aagaccattt ctatcccgag atcatcgacc
     2761 cccagtcggg cgaagtgctg cccgagggtg agaagggcga gctcgtcctg acctcgctca
     2821 ccaaggaagc gcttccggtc atccgctacc gtacgcgcga cctgacgcgg ctgctgccgg
     2881 gcagcgcgcg caccatgcgc cgcatcgaga agatcaccgg ccgctccgac gacatgctca
     2941 tcatccgcgg ggtgaacgtg tttccgtcgc agatcgagga gctcgtcctc aagcagcccg
     3001 gcctcgcccc gcactacgtg ctcgaagtca ccaagcacgg cccgctcgac tacctcgccg
     3061 tcatcgtcga gggcgaagcg ggagcctgca aggcgctcga gcaccacatc aagtcgagga
     3121 tcggcatcag cgccgaggtg cggctcggca ctgtcgagcg ctcgatcggc aaggcaaagc
     3181 gggtcatcga taagcggccg aagtagttta ttcgagcacg ccgagcctga cgggccgcat
     3241 caccgccgcg ccggcggaat gcacctcgag cttgccgtag gttttcttga cgtgcgtcac
     3301 cacggtatgc ggcgagatcc cgagccgtat cgctgcctgc agatagctgc agcccgacgc
     3361 gagcagacga agcacgtcga gttctcgcgg agtgagcgca gtcatggtgg ctcccttcat
     3421 tcgacgacga ccgagacgtt ggtcttgcgg tagctgtaga aaacgatgtt cgagcgcgtc
     3481 tcttcgaccg acgcgaccag cgcgatgtag ctgtcgcgcc agcgccattt cttgttgcct
     3541 accgttacct cggcgcgcac cctgtcgtcg tcgcggcgct cgccgaacag cggatcggcg
     3601 tcggcgtact tcctcgagcc gtaggtcagg ctcgcgttga ccaggaacat gtactgcggc
     3661 cagaacgtgc tgatgccggg cgcgatatcc cagccgtagt aagagtaggg ccgctcctcg
     3721 gcgtgcgagc cttcgaccga accgcgcaga gtgccggcga cggtctgcga cagctcgcgg
     3781 cgcagcgaca acgccccgct gcgataggtc gagtcgagat actcgaagtc gggataggta
     3841 accctgccga gcttgagctc gcccgccagc cggtacgact ccgactgcgc gagcaccgcg
     3901 tccaggccaa catagggaaa gcggtagagt ccgttgccgc cgagcgtgcc gaactccagg
     3961 cccgccttgc cgcggacgcg ccccgactcg accaggttct tcatcagccc ggcgtcgacc
     4021 gtgagccggt cgaagtcctg ctcggggtaa tcgacatagg aggcggtgag atagccggcc
     4081 gcgccgatcg gcttgagcgg cagccaaccc tgtgccgaat agcgcaggcc ggtgactttc
     4141 tcgggcgcgg cgctcgaggt cacgagcaca ccgccgatgg cgaactcctt ctgccgcgcg
     4201 atgttgcccg ggttggagtc cgagacgacc gtcacgccga gcttgaggta cccggtgcgc
     4261 tcctcgatct cgcgcacgaa cagcgcgatg ttgtcgcgca cgcgccaggg agtgtccgac
     4321 tgcaccgaga cttccttgaa cagccgcttt gcttcctcgt gcttcgcctg gaggaagagc
     4381 gtgcgcgcga gctcgagctt cacgcgcgga ctgtcggtgt gctcgagcat tgcgcggaag
     4441 atcgcctcgg cgtgcgcgag atctcccgcc tgctgcgcct gcattgcgtg tacgaacgcc
     4501 cgctgcagtg cctcctcgtc cgcgcaggcg ggcgagacag cagcgaccag cagggcgatg
     4561 aggacggcgc gcatcggcta ccttttcgcc ccgaagccgc cggtaaagag ctcgttgccg
     4621 gcgccctgca accggaacgc gccgccaagc tcctcggcat taggcccata gaactggccc
     4681 ttcgaggagc ctgtcatgct gccgcccgcg ttcactactg cgccggagaa gctcgcttgg
     4741 cctggcgcgt aagtgagcgt cccgcgcagg tcgagctgcg gagccgcgct accctggacc
     4801 gtagtgccgc ttgacgcgaa gccgagcgag cgcgagctga agttcgcatt cacggtcaca
     4861 tcggcgccgg ccgtggcgcc tgcgccggtc ggggcgacgt aaaagccgat cagcttgccg
     4921 gtgaaagtcg cgctgcc
//
LOCUS       DASUNT010000293         2229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_303556, whole genome shotgun sequence.
ACCESSION   DASUNT010000293 DASUNT010000000
VERSION     DASUNT010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303556"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VFX94_05865"
     CDS             complement(<1..333)
                     /locus_tag="VFX94_05865"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX5767744.1"
                     /translation="MNVALQLLQSALVVLAAPLVLGWVNICRAWLQNRSAPSILLPYY
                     TLAKLFHKDAVIANTTSPMFRITPYVLFGCMALAAGIVPVLATDLPFAPAADIIALVG
                     LFATARVFA"
     gene            complement(330..>2229)
                     /gene="hyfB"
                     /locus_tag="VFX94_05870"
     CDS             complement(330..>2229)
                     /gene="hyfB"
                     /locus_tag="VFX94_05870"
                     /inference="COORDINATES: protein motif:HMM:NF005086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HEX5767745.1"
                     /translation="RWLFPLGAALGLVLAALGARAVWSAPETLVLPLGLPELPFHLRM
                     DPLAGFFLLLLGAVAAGISVYARGYFRDETPGRLRLICLQYHVFLAAMAFVILADDAY
                     LFMVAWETMALSSYFLVTTDHRVPAIRSAGFLYLLIAHIGAIAILLCFGLLHGGQGDY
                     RFAALRGAELSPLWTHVAFALAFVGFGAKAGVIPLHAWLPEAHPAAPSPVSALMSGVM
                     IKTAIYGLVRVTYDLIGEVYWQWGLAVLLVGAGTTLFGVLFALMQHDLKRLLAYHSVE
                     NIGIILLGLGMSLVFFGFGHPQAGALGLIAALYHTLNHAVFKALLFLGAGSILHASGL
                     RDLNHMGGLARTMPATAFHFLVGALAISALPPLNGFVSEWLTFQSALQAPLLEHSVMR
                     SLVPLFAATLALAGALTAMCFVKVYGIAFLGQPRHKASVSRDAGPSERLGLAWLSAGC
                     LVLGLFPAGMLQVLNQVGASLTGHGLSAEALESDWLWLVPTHPAEASYSPLIFFVVVL
                     AVVLLTVRLVRLFYHGRVRRADPWDCGFPEQTPRMQDTADAFGQPIGLVFGPLYLMKR
                     SLPGPEDPTPKYSMKIEDRHWPWLYLPVVHAVEFASARIGRLQRGRISTYLLYSFLTL
                     IALLVFVR"
BASE COUNT          434 a          707 c          821 g          267 t
ORIGIN      
        1 ggcgaacacg cgcgcggtcg cgaacagccc gaccagcgcg atgatgtccg ccgctggcgc
       61 gaacggcagg tcggtggcga ggaccggcac gatgcccgcg gcgagcgcca tgcagccgaa
      121 gagcacgtag ggagtgatgc ggaacatcgg cgaggtcgtg ttggcaatca ccgcgtcctt
      181 gtggaagagc ttcgccagcg tgtagtaggg cagcaggatg ctgggcgccg agcggttctg
      241 cagccacgct cgacagatgt tcacccagcc gagcacgagc ggcgcggcga gcaccacgag
      301 agccgactgc agcagctgca gggcgacgtt catcgcacga acacgaggag cgcgatcagc
      361 gtaaggaagc tgtacagcag gtaggtgctg atgcgcccgc gctgcagcct cccgatcctg
      421 gccgaggcga actcgaccgc gtgcacaacc ggcaggtata gccagggcca gtgccggtcc
      481 tcgatcttca tcgagtactt gggcgtgggg tcctcggggc cggggaggct gcgcttcatg
      541 aggtacagcg gcccgaagac gaggccgatc ggctggccga acgcatcggc ggtgtcctgc
      601 atgcggggcg tctgctcggg aaagccgcaa tcccatggat cggcgcgccg cacgcggccg
      661 tggtagaaga ggcgcaccag ccgcaccgtg agcagcacca ccgctaggac gacgacgaag
      721 aaaatgagcg ggctgtagct cgcctcggcg gggtgcgtcg gcacgagcca cagccagtcc
      781 gactccagcg cttcagccga tagaccgtgg ccggtgagcg aggcgccgac ctggttgagc
      841 acctgcagca tgccggccgg aaagaggccg agcaccaggc agccggcgct gagccacgca
      901 agccccaggc gctcgctcgg accggcatcg cgggacacgc tcgccttgtg gcgcggctgt
      961 ccgaggaacg cgatgccgta gaccttgacg aagcacatcg ccgtgagcgc gccggcgagc
     1021 gcgagcgtgg cggcgaagag cggtacgagg ctgcgcatca cgctgtgctc gaggagcggc
     1081 gcctgcagcg cgctctgaaa ggtcagccat tcggagacga agccgttgag cggcggcagc
     1141 gccgagatgg caagcgcgcc gacgaggaaa tggaacgcgg tggccggcat cgtgcgtgca
     1201 agcccgccca tgtggttgag gtcgcgcagg ccgcttgcat gcaggatcga gcccgcgccg
     1261 aggaagagca gcgccttgaa caccgcgtgg ttgagcgtgt gatagagcgc ggctatgagc
     1321 ccgagcgccc ctgcctgcgg gtggccgaag ccgaagaaca cgagcgacat ccccaggccg
     1381 agcaggatga tgccgatgtt ttccaccgag tggtaggcga gcaggcgctt gagatcgtgc
     1441 tgcatcaagg cgaacagcac gccgaaaagc gtcgtccccg cgccgacgag gagcaccgcg
     1501 aggccccatt gccagtacac ctcgccgatc aggtcgtagg tgacgcgcac caggccgtag
     1561 atcgcggtct tgatcatcac gccgctcatc agggccgaga ctggggaggg cgctgcggga
     1621 tgcgcctccg gcagccaggc gtgcagagga atgaccccgg ccttcgcgcc gaagcccacg
     1681 aaggcgaggg cgaaggcgac gtgggtccag agcggggaga gctccgcgcc gcgcagcgcg
     1741 gcgaacctgt agtcgccctg gcctccgtgc agcaggccga agcagagcag gatcgcgatc
     1801 gcgccgatgt gcgcgatcag caggtacaga aacccggcgc tgcggatcgc aggcacgcgg
     1861 tgatcggtgg tcacgaggaa gtacgacgaa agcgccatcg tttcccacgc caccatgaag
     1921 aggtaagcgt cgtcggcgag gatcacgaat gccatcgcgg cgaggaacac gtggtactgc
     1981 aggcagatga ggcgcagccg tcccggggtc tcgtcgcgga agtagcctct ggcgtacacc
     2041 gagattcccg ccgcgacagc gccgagcagg agcaggaaga acccggcgag cggatccatg
     2101 cgcaggtgga acggaagctc cggcaggccg agcggcagca cgagcgtctc gggcgcgctc
     2161 cacacggcgc gcgcgccgag cgccgcgagc accaggccga gcgccgcgcc gagcggaaag
     2221 agccagcgc
//
LOCUS       DASUNT010000294         9154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1268213, whole genome shotgun sequence.
ACCESSION   DASUNT010000294 DASUNT010000000
VERSION     DASUNT010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268213"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VFX94_05875"
     CDS             complement(<1..490)
                     /locus_tag="VFX94_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767746.1"
                     /translation="MYTPAAAIDSEVLLEEDGFLYLDANEVPRESVVALLSESFSREP
                     MARALGASARDLAPFVRRFMPECTSNGLSVVAVRDDDPQALVAAFLSRDFKSPVPEDI
                     MEDCPWFMPVGQALMSVGEVYEGRHPQLKRGDVADLWMVGTDARYARRGLARRMFMLC
                     MRL"
     gene            complement(549..1454)
                     /locus_tag="VFX94_05880"
     CDS             complement(549..1454)
                     /locus_tag="VFX94_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5767747.1"
                     /translation="MTLIHNPGAGKQGKNDADRLLALVRDAGHEVRYQSCKADGWEEA
                     LQQPAELVAVAGGDGTVSRVARHMLGRGIPVAALPSGTANNISRSLGLVERPFEELVR
                     AWPQSRRVKLDVGVAEGPWGRRHFVEGIGAGLFARLLATGGDAIKQRRMKPDEKVAHA
                     LQRLKLRAQRARPLGLKAWIDGEEISGKYLLLEAVTIPYVGSNLFLAPDSKPGDGHLE
                     VVLVTEAERSRLVNYLESWQQNRERLAVLPSHRGQSLTFEWTGFEIHIDDKLWPQKGD
                     AAKPPARVEARLEGEVEFLVPGRQD"
     gene            1483..2235
                     /gene="surE"
                     /locus_tag="VFX94_05885"
     CDS             1483..2235
                     /gene="surE"
                     /locus_tag="VFX94_05885"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX5767748.1"
                     /translation="MRILVTNDDGVYSPGIAALAQVASRFGEARVVAPDVEMSSASHS
                     ITASRPVTMKRTALPGLEAYRVNGTPSDCVMLGTTLWEKVDLVLSGINIGTNLGNALW
                     HSGTLAGAKQAALLGLRGVAFSAPSTETEQPDFETLKPWAATVLEALLDAPEPELVNV
                     NFPARAPRGTVWTRQSMRHYDGKVVPGKDPMGRTHYWFTVVPVEATEEGTDRWALEQG
                     YVSMTPLRLDLTDHDALGRMRKLEAWKRLKFA"
     gene            2232..3014
                     /locus_tag="VFX94_05890"
                     /pseudo
     CDS             2232..3014
                     /locus_tag="VFX94_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            complement(3109..4266)
                     /locus_tag="VFX94_05895"
     CDS             complement(3109..4266)
                     /locus_tag="VFX94_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEX5767749.1"
                     /translation="MSSSPSSAFFASAPRGLESLLQDEISSLGGKNAAVVPGGVSFQG
                     GWDTCYRVNLWSRIASRVLWKVHDFAYRNEDDLYDAVKALEWSRYFSVERTLRVNLTA
                     QKSPLRSLEFATLRVKDAVCDRFREALGRRPDVERTVPDVRVHVFLERERGMLYLDTS
                     AEPLFKRGWRAGQAEAPLRENLAAGIVMLTGWRPSEPLFDPMCGGGTLLIEAAAIARR
                     RAPGARRDFGFQKLSNFDPSVWESVKGEASEPDANPVLHGSDSDPQALKAARRNLAAA
                     GVERWVKLERADVLERAAPAQAGVMVMNPPYGERMGSAEELARFYPKLGDALKRNFVG
                     WRCYVFTADLRLPKLIRLEPSRRTPLWNGALECRLYEFEIVQGRPTRGSRR"
     gene            complement(4247..4663)
                     /locus_tag="VFX94_05900"
     CDS             complement(4247..4663)
                     /locus_tag="VFX94_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX5767750.1"
                     /translation="MLWIKSFHIVFMVTWFAGLFYLPRLFVYHALSQDRPSIDRFKVM
                     ERKLFWGIMTPGAALTIIFGAWLWLGWFRASSNWLYAKLVLVAILVAYHLWCWRLMRN
                     FAEERNTRSHLWFRWFNEFPVLVLVATVLLVVFKPF"
     gene            complement(4666..5415)
                     /locus_tag="VFX94_05905"
     CDS             complement(4666..5415)
                     /locus_tag="VFX94_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5767751.1"
                     /translation="MRWMSVWRRNMLVWRKLAAAAMLTNLADPLIMLFGLGYGLGALL
                     PSVQGMSYIAFFAAGQLCTATMFTASFEAMFSSFARMHGQKTWDAILYAPLIIDDIVA
                     GEIVWAASKALLTGSTILGVAAIFGLASSPWVLLALPAAFLVGLAFSAVGLIMCVLAR
                     GWDFFSYYMTLVMTPMMMISGVFFPAEQLPGPLVAVAKALPLYHGVQIVRPLIAGGPP
                     PGLWLHVAVLLAYAAAGYAAAIYFARRRFSS"
     gene            complement(5418..6320)
                     /locus_tag="VFX94_05910"
     CDS             complement(5418..6320)
                     /locus_tag="VFX94_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5767752.1"
                     /translation="MSALAVRGLRKSYGALDVVSGLDLAVEPGECFGLLGPNGAGKTT
                     TLKLCLGLIEPDGGDIELLGEPVPQRAREARARVGVVPQFDNLDPDFSVEENLVVYGR
                     YFGIDPRQIAPRIPALLEFAGLAGRSDAKILTLSGGMKRRLTLARALVNDPQLVFMDE
                     PTTGLDPQARHLIWERLRRLTHEGKTLVLTTHFMEEAERLCHRLAIMDRGRIIAEGSP
                     RALIAQHIEPQVLEVHGPGYESWMERARTLAPRIERVGDTVYCYGEEIEPLLKTLSGD
                     LSYLHRPASLEDVFLKLTGRDLRD"
     gene            complement(6317..6910)
                     /locus_tag="VFX94_05915"
     CDS             complement(6317..6910)
                     /locus_tag="VFX94_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009908400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5767753.1"
                     /translation="MRASTRRQFIAGPADRIECALDSPDGASRGVALIAHPHPLFGGT
                     LDNKVVQTLARAFVELGYESWRPNFRGVGKTDGVHDEGRGELEDLAAVLSHIRTERFV
                     LAGFSFGASLVARLAERAKPEWLVLVGIGVTRLAAPPVPADTLVIHGENDDTVPLAAV
                     LEWARPQELPLVLVPGADHFFHRKLHVLKRIILANWK"
     gene            complement(6911..7219)
                     /locus_tag="VFX94_05920"
     CDS             complement(6911..7219)
                     /locus_tag="VFX94_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX5767754.1"
                     /translation="MSYYSKHVFFCCNQRDGGRTCCNDKGASRMRDYAKGRIKDLGLA
                     GPGRIRVNQAGCLDRCEEGPCIVVYPEAVWYTYVDQSDVDEIIEEHLQNGRIVERLRM
                     "
     gene            complement(7232..8389)
                     /locus_tag="VFX94_05925"
     CDS             complement(7232..8389)
                     /locus_tag="VFX94_05925"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX5767755.1"
                     /translation="MAWCSAPSCRARPFSRPTTTASTERDITALLAPRGWTLARILFL
                     VYVALVAYASLYPLSGWRDVGLSPFAFLGEPWPRYFTRFDIVANVAGYLPFGFLAIAT
                     LQPRLRGLAAFAVSVAGASVLSLVLEAAQSYLPARFPATADVLCNLGGAALGAALGLH
                     FAPLMTGGPLSRWRAGTFVAGGGTDFGLVLIGLWLFIQLNPATLLFGAGDLRDLLTPF
                     EGRARSPEFFVSIEAFTTAAHLVAVALILAALVAPGRPVRGLVLGTVLAALTVKTAAV
                     AIVLRAESVFAWLTHGAQLGLVIGIAAALGAVALPRTVRLAGAAVLLMAATVLVNLSP
                     PNPYLAASLKVWQQGHFLNFNGLTRLVSAAWPFIALGYLIYLASRRREALG"
     gene            complement(8319..>9154)
                     /locus_tag="VFX94_05930"
     CDS             complement(8319..>9154)
                     /locus_tag="VFX94_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX5767756.1"
                     /translation="GKVIAAASEAGKFEFWPKGGTKEWLAFLAAWMTLAIGSIPQQDV
                     FQRVTSAKDETTAVRGTLIGAAVYFCFAFVPIFIAYSALVIDPGYEKLFASEDAREIQ
                     RILPDLILNKTPMWAQVLFFGALLSAILSTASGAIIAPTSLFTENVIRPFVPGMSDRQ
                     FLLLLRVILVTFTLAALLFALHSKSTMYEMVQNAYKVTLVSCIVPLAAGIFWKRANVP
                     GAVLSVVFGLASWTIAEWVAPEGTVPPQLVGLGFSIVGMVLGSLVPRPAVQPAHHHGK
                     H"
BASE COUNT         1647 a         3067 c         3042 g         1398 t
ORIGIN      
        1 cgaggcgcat gcagagcatg aacatccggc gtgccagtcc gcgccgcgcg tagcgagcgt
       61 ccgtgccgac catccacaga tcggcgacgt cgccgcgctt gagttgcgga tgcctgccct
      121 catagacctc gccgacgctc atgagcgcct ggcccaccgg catgaaccag ggacagtcct
      181 ccatgatgtc ctcgggcacc ggcgacttga aatcacggga caggaaggcg gcgacgagcg
      241 cctgcggatc atcatcgcgt accgcgacga ccgagagtcc gttgctcgtg cattcgggca
      301 tgaaccgcct gacaaaagga gcgaggtcgc gcgcggatgc accaagcgcc cgcgccatcg
      361 gctcgcggga gaaactttcg ctcagtagcg cgaccacgct ctcccgcggc acctcattcg
      421 cgtccagata gagaaaaccg tcctcttcca ggaggacttc cgaatcgatc gccgccgctg
      481 gcgtatacat gcagcaagcc taccccttgc ctgtatctgg caattttccg tctggtctgc
      541 aatttgtttc agtcctgtcg gccggggacc agaaactcga cctcgccttc caggcgggcc
      601 tccacgcgcg ccggcggctt ggccgcgtct cccttctgcg gccacagctt gtcgtcgatg
      661 tggatttcga aaccggtcca ttcgaaggtg agggactgcc cgcggtgcga cggaagcacg
      721 gcgagacgct cgcgattttg ctgccagctt tccaggtagt ttacgagccg cgagcgctcc
      781 gcctcggtga cgagcacgac ttcgagatgc ccgtcgcccg gcttgctgtc cggtgcgagg
      841 aaaaggttgg agccgacata cgggatggtc accgcctcga ggagcaggta cttgcccgag
      901 atctcctcgc catcgatcca cgccttgagc ccgagcggcc tggcgcgctg cgcgcggagt
      961 ttcagccgtt gcagcgcgtg cgcgaccttt tcatcgggct tcatccggcg ctgcttgatg
     1021 gcgtcgccgc ccgtcgcgag caggcgcgcg aagagcccgg cgccgattcc ctcgacgaag
     1081 tgccgcctgc cccacgggcc ttcggcgacg ccgacgtcga gctttacgcg gcgcgactgc
     1141 ggccacgcgc gcacgagctc ctcgaagggc cgctccacca gcccgaggct tctcgagatg
     1201 ttgttcgccg tgcccgaagg aagcgccgcg accgggatgc cgcgcccgag catgtgccgc
     1261 gcgacgcgac tcacggtgcc gtcgccgccc gcgaccgcga cgagctccgc gggttgctgc
     1321 agcgcctctt cccagccgtc ggccttgcac gactggtagc gcacctcgtg gccggcgtca
     1381 cgcaccaggg cgagaagcct gtcggcgtcg ttcttgccct gctttcccgc gccggggttg
     1441 tgaatcagcg tcactcgcac gctatttgct ccgttcttcc atatgcgcat cctcgtaacg
     1501 aacgacgacg gagtctacag tcccggcata gccgcgttgg cgcaagtggc ttcccggttc
     1561 ggcgaggccc gggtcgtcgc cccggacgtc gaaatgtcgt cggcaagcca ttcgatcacc
     1621 gcgtcgcgac cggtgaccat gaagcgcacg gcgcttccgg ggctcgaagc ctaccgggtg
     1681 aacggcacgc cgagcgactg cgtgatgctc ggcactacgc tctgggaaaa ggtcgacctg
     1741 gtcctctcgg gcatcaatat cggcacgaac ctcggcaacg ccctctggca ttcgggtacg
     1801 ctcgccggcg cgaagcaggc cgcgctcctt gggctgcgcg gtgtcgcctt cagcgcgccg
     1861 tccaccgaga ccgagcagcc ggacttcgag acgctcaagc catgggccgc gaccgtgctc
     1921 gaggcgctgc tcgacgcgcc tgagcccgag ctcgtgaacg tcaacttccc ggcgcgcgcg
     1981 ccgcgcggca ctgtctggac gcgccagtcc atgaggcact acgacggcaa ggtggttccc
     2041 ggcaaggacc cgatgggccg cacgcactac tggttcacgg tggtgccggt cgaggccacc
     2101 gaagagggca ccgatcgctg ggcgctcgag cagggctatg tctcgatgac gccgctgcgc
     2161 ctggacctca ccgaccacga cgcgctcggc cgcatgcgca agctcgaggc ctggaagcgg
     2221 ctgaagttcg cttgatccgg gtcggcattt ccggctggcg ctacgagccc tggcgccgcg
     2281 tctggtatcc cgaaggactc gcgcagcacc gcgaactcga gttctgcggc cggcatttcc
     2341 ccacggtgga aataaacggc tcgttctatt cgctgcagcg gccgcaatac tacgagcagt
     2401 ggtatcgcga tacgccgccc ggcttcgtat tcgcggtgaa gggctcccgc tacctcactc
     2461 acatgctgcg gctcacgaag atcgagaagc cgcttgcgaa cttcttcgcc tccggcatct
     2521 tcgccctgcg cgagaagctc gggccgtttc tgtggcaatt ccccccgatg ttcatctacc
     2581 gaaaagaacg actggagcaa ttcttctcgc ttcttcccag gaccacgggg caggcgctgg
     2641 cgctcgcccg ccggcgcgat gcgcgcatgt tcggtcgctc gcgcctcgcc atcgatgccg
     2701 accgtgcgct gcgccacgcg gtcgagatcc ggcatccgag cttcatgaac gatgactttg
     2761 tttcgctcct gcgcaatcac cgcatcggcc tcgtggtcgc ggatacggcc ggaaaatggc
     2821 ccaagatgtt ccatgtcact tccgacttcg tctacgtgcg gctgcacggc gacgtgaaga
     2881 tctacacgag cggctactcc gagcgggcgc tggcgagctg ggcaaggcgc atccgcaact
     2941 gggacaggga cggacgcgat gtgtacgtct acttcgacaa cgacgtgaag gtcaaggcgc
     3001 ctttcgatgc gctcaacctc atgcgcaagc tgcgcctcga atggggagta ccctggcaca
     3061 aggctcgcgc acgcattccg cggcttaaat acgcctatgg accgcgggtc accggcggga
     3121 accccgcgtg ggacgacctt gcacgatctc gaactcgtag agccggcatt cgagcgcgcc
     3181 gttccagagc ggcgtacggc gcgagggctc gaggcggatc agcttcggca gcctcaggtc
     3241 ggcggtgaaa acgtagcagc gccagccgac gaaattgcgc ttcagcgcgt cgccgagctt
     3301 cggatagaag cgcgcgagct cctcggcgct tcccatgcgc tcgccgtagg gcgggttcat
     3361 caccatcacc cccgcctgcg cgggggccgc gcgctcgagc acgtcggcac gctccagctt
     3421 tacccagcgt tcaaccccgg cggcggcgag attgcggcgc gccgccttca acgcttgcgg
     3481 gtcactgtcg ctgccatgca agacgggatt cgcatcgggc tcggatgctt cgccctttac
     3541 gctctcccaa acggaaggat cgaagttgct taacttctga aaaccgaaat cgcgccttgc
     3601 ccccggcgcg cggcggcgtg cgatcgcggc cgcttcgata agcagcgtgc cgccaccgca
     3661 catcgggtcg aacagcggct cgcttggacg ccagccggtg agcatgacaa tgcctgcggc
     3721 gaggttctca cgcagcggcg cctccgcctg ccccgcgcgc cacccgcgct tgaagagcgg
     3781 ctctgcggaa gtgtcgagat aaagcatgcc tcgctctctt tcgaggaaga catggacccg
     3841 cacgtccggg actgtgcgct cgacgtccgg gcgcctgcca agcgcctcac ggaagcggtc
     3901 gcacaccgcg tccttcacgc gcagcgtcgc gaactcgagg ctccgcagcg ggcttttctg
     3961 cgcggtcagg ttgacgcgga gggtgcgctc tacgctgaag tagcgcgacc actccagtgc
     4021 tttgaccgcg tcgtagagat catcctcgtt gcggtaagcg aagtcgtgaa ccttccacag
     4081 cacccgcgag gcgatgcgcg accagaggtt tacgcggtag caggtgtccc agccgccctg
     4141 gaacgaaacg ccgccgggca cgacggcggc attctttcct cccaacgagg aaatctcgtc
     4201 ctgcagaagc gattcgaggc cgcgcggcgc gctagcgaag aaggcgctag aagggcttga
     4261 agacaacgag cagcaccgtg gcgacgagaa cgagcaccgg aaattcgttg aaccagcgaa
     4321 accacaggtg actgcgggtg ttgcgctcct cggcgaagtt gcgcatcagc cgccagcacc
     4381 agaggtggta ggccacgagg atggcgacga gcacgagctt cgcgtatagc caattcgagc
     4441 tcgccctgaa ccagccgagc cacagccagg cgccgaagat gatggtgaga gccgcgcccg
     4501 gcgtcatgat tccccagaag agctttcgct ccatcacctt gaaacgatcg atcgacggtc
     4561 gatcctgtga gagcgcgtgg tagacaaaaa gccgcggcag gtagaaaagc ccggcgaacc
     4621 acgtgaccat gaagacgatg tggaaggact tgatccagag cacggtcagg atgagaagcg
     4681 ccgccgtgcg aaatagattg ccgcggcgta gccggccgca gcatacgcga gcagcactgc
     4741 cacgtgcagc cacaggccgg gcggcggacc gccggcgatc agcgggcgca cgatctgcac
     4801 cccgtggtag agcggcagcg cctttgccac ggcgacgagc gggccgggca gctgctcggc
     4861 ggggaagaac acccctgaga tcatcatcat cggcgtcatg acgagagtca tgtagtagct
     4921 gaaaaagtcc cagccgcggg cgagcacgca catgatgagg cccaccgccg agaacgccag
     4981 gccgacgagg aaagccgccg gcagcgcgag cagcacccag ggcgagctcg ccagcccgaa
     5041 gattgcggcg acgccgagaa tcgtcgagcc ggtcagcaac gccttgctcg ccgcccagac
     5101 gatctccccg gccacgatat cgtcgatgat gagcggcgcg tagagaatcg cgtcccaggt
     5161 cttctgcccg tgcatgcggg cgaaggacga gaacatcgcc tcgaacgacg cggtgaacat
     5221 ggtggccgtg cagagctgcc cggcggcgaa gaacgcgatg tacgacatcc cttgcaccga
     5281 aggcagcagc gcgcccaggc cgtatcccag cccgaacagc atgatgagcg ggtcggcgag
     5341 gttggtcagc atcgcggccg cggcgagctt gcgccagacc agcatgtttc tgcgccacac
     5401 cgacatccag cgcatgctca atctctcagg tcgcggccgg tcagcttgag gaagacgtcc
     5461 tccaggctcg ccggtcggtg cagatagctg agatcgcccg aaagcgtctt cagcaacggc
     5521 tcgatctcct cgccgtagca gtacaccgtg tcgcccacgc gctcgatgcg cggtgcgagc
     5581 gttctggcgc gctccatcca gctctcgtag cccggcccgt gcacctcgag cacctggggc
     5641 tcgatatgct gcgcgatgag cgcgcggggc gagccttccg cgatgatgcg gccgcgatcc
     5701 atgatcgcca gccggtggca gagccgctcg gcttcctcca tgaaatgcgt ggtgagcacg
     5761 agcgtcttgc cttcgtgcgt cagccgacgc agccgctccc agatgaggtg gcgcgcctgc
     5821 ggatcgagcc cggtggtggg ctcgtccatg aagacgagct gcgggtcgtt gacgagagcg
     5881 cgcgcgagcg tcaggcggcg cttcatcccg cccgagagcg tgaggatctt cgcatcgctg
     5941 cggccggcaa gccccgcaaa ctcgaggagc gccggaatgc gcggagcgat ctgcctcgga
     6001 tcaatgccga aatagcgtcc ataaaccacg aggttctcct cgacgctgaa atccgggtcg
     6061 aggttgtcga attgcggcac cacgccgacg cgcgcgcgcg cttctcgcgc cctctgcggc
     6121 accggctcgc cgagaagctc gatgtcgccg ccatcgggct cgatcaggcc gaggcacagc
     6181 ttgagcgtgg tcgtcttgcc ggcgccgttc ggaccgagca ggccgaagca ctcccccggc
     6241 tccaccgcga gatcgagtcc cgacacgacg tcgagcgcgc cgtaagactt gcgcagcccg
     6301 cgcaccgcga gcgcgctcat ttccagttcg ccaggatgat gcgcttgagg acgtggagct
     6361 tgcggtggaa gaagtggtcg gcgccgggca cgagcaccag cggcagctcc tgcggccggg
     6421 cccattcgag gaccgccgcg agcggcacgg tgtcgtcgtt ctcgccgtgg atcaccagcg
     6481 tgtccgcggg cacaggcggg gccgcgagcc gcgtgacgcc gatgccgacc aggacgagcc
     6541 actcgggctt cgcgcgctcc gcgaggcgcg cgacgagcga tgcgccgaag gaaaaaccgg
     6601 ccagcacgaa gcgttcggtt cgaatgtggc tcagaacggc ggccagatcc tccagctcgc
     6661 cgcggccttc gtcgtgaaca ccgtcggttt tccccacgcc gcggaagttg ggacgccacg
     6721 actcgtagcc gagctcgacg aacgcgcgcg cgagggtctg cactaccttg ttgtcgagcg
     6781 tgccgccgaa gagcggatgc ggatgcgcga tgagcgcgac gccacggctt gcgccgtcag
     6841 ggctgtccaa cgcgcactcg atgcggtcgg ccgggccggc gatgaactga cgacgggtgc
     6901 tcgcccgcat ctacatcctg agacgttcga cgatgcgccc gttctgaagg tgctcctcga
     6961 tgatctcgtc gacgtcggac tggtcgacat acgtgtacca gaccgcttcc gggtacacca
     7021 cgatgcacgg gccttcctcg catcggtcca ggcagcccgc ctggttgacg cggatcctgc
     7081 ccgggccggc gagacccagg tccttaatgc gccccttggc gtagtcgcgc attcgcgagg
     7141 cgcccttgtc gttgcagcag gtgcgcccgc cgtcgcgctg gttgcagcag aagaagacgt
     7201 gcttcgagta ataggacatg agcccggcat tctagccgag cgcttcgcgc cgcctggacg
     7261 cgaggtaaat gagatagccc agcgcgatga agggccatgc ggcgctcacc agccgcgtga
     7321 ggccgttgaa gttgagaaag tgaccctgct gccacacttt gagcgatgcg gcgaggtagg
     7381 gattcggcgg cgagagattc acgagaaccg tcgcggccat caacagcact gcggcgccgg
     7441 cgagccgcac ggtgcgcggc agcgccactg cgccaagcgc agcggcgatc ccgatcacaa
     7501 ggccgagctg cgcgccgtgc gtgagccacg cgaaaacgct ctcggcgcgc agcacgatcg
     7561 ccaccgccgc cgtcttcacc gtgagggcgg cgagcaccgt gccgagcacc aggccgcgca
     7621 cgggacgccc cggtgcgacg agcgccgcca ggatcagcgc caccgcgacc aggtgcgccg
     7681 cggtggtgaa agcttcgatc gacacgaaga actcgggcga gcgcgcgcgc ccctcgaacg
     7741 gcgtcagcag gtcgcgcagg tcgccggcgc cgaagagcag cgtcgccggg ttgagctgta
     7801 tgaagagcca cagcccgatc agcaccaggc cgaaatccgt gccgccgccg gcgacgaacg
     7861 tgcccgcgcg ccagcgcgag agcggcccgc cggtcatgag gggagcgaaa tgcaggccga
     7921 gcgccgcgcc gagcgcggcc ccgccgaggt tgcatagcac gtcggcggtg gccggaaaac
     7981 gcgccggcag gtagctctgc gcggcttcga ggacgaggga cagcaccgat gcgcccgcca
     8041 ccgacacggc gaatgcggcg agcccgcgca gccgcggctg cagggtcgcg atcgcgagga
     8101 agccgaacgg aagatagccc gcgacgttgg caacgatgtc gaagcgggtg aaatagcgcg
     8161 gccagggctc gcccaggaag gcgaacggcg agagccctac gtcgcgccag cccgaaagcg
     8221 ggtaaagcga agcgtaggca acgagcgcga catagaccag gaacaggatg cgcgcgagcg
     8281 tccagccgcg cggcgcgagg agcgcggtga tgtcgcgctc agtgcttgcc gtggtggtgg
     8341 gccggctgaa cggccgggcg cggcacgagg gagccgagca ccatgccaac gatcgagaag
     8401 cccaggccga cgagctgcgg cggcacggtg ccctccggcg cgacccattc ggcaatggtc
     8461 caggaagcga ggccgaacac caccgacagc accgctcccg gcacattggc gcgcttccag
     8521 aagatgccgg cggcgagcgg cactatgcag gaaacgagcg tcactttgta ggcgttctgc
     8581 accatctcgt acatcgtgct cttcgaatgc agcgcgaaaa gcagcgccgc cagcgtgaaa
     8641 gtgacgagga tgacgcgcag caggaggagg aactgccgat cgctcatgcc ggggacgaac
     8701 ggccggatga cgttctcggt gaagagcgac gttggcgcga tgatcgcgcc gctggcggtc
     8761 gacaggatgg ccgagagcag cgcgccgaag aacagcacct gcgcccacat cggggtcttg
     8821 ttgaggatca agtccggaag gatgcgctgg atctcgcgtg cgtcctcgct cgcgaagagc
     8881 ttttcgtagc ccgggtcgat caccagcgcc gagtaggcga tgaagatcgg cacgaacgcg
     8941 aagcagaagt acaccgcagc gccgatcagc gtaccgcgca ccgcggtcgt ttcgtccttc
     9001 gccgaagtga cgcgctggaa cacatcctgc tgcgggatgg agccgatggc gagcgtcatc
     9061 caggcggcga ggaaggcgag ccattccttc gtgccgcctt tgggccagaa ttcgaacttg
     9121 cccgcttccg acgcggccgc gatcaccttg cccg
//
LOCUS       DASUNT010000295         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1091849, whole genome shotgun sequence.
ACCESSION   DASUNT010000295 DASUNT010000000
VERSION     DASUNT010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091849"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..519
                     /locus_tag="VFX94_05935"
                     /pseudo
     CDS             61..519
                     /locus_tag="VFX94_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
     gene            complement(431..1780)
                     /locus_tag="VFX94_05940"
     CDS             complement(431..1780)
                     /locus_tag="VFX94_05940"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HEX5767757.1"
                     /translation="MRPRNAEPVIVAGGGIGGLAAALALARKGFRSVVLEQASQFGEI
                     GAGIQIAPNAWHALDALGVGALVKKEAVFIEHLLMFDGVSGEKVIDIPLDRRFAKRFG
                     NPYAVTHRADIHGSLLDGCKALPDLIELRTSTRVTGFEDEGTGVTVRTEKDAIRGIAL
                     VGADGGKSVIRERIVGDTLPLITGHMCYRAVLDINDVPKDLRFAAATLWAAHNAHIVH
                     YPLRGWKLFNLVATIIGKHTSGGHNELAHPEEVLPFFSHYCDKPLKLMRTPKEFRRWM
                     LLHREPVDNWSRGRVTLLGDAAHFMLQYMAQGAAMAMEDAVCLGLCAEELDGDFESVF
                     LEYQKRRMVRATRVQVSANRLVGMIFHVPDGLERLVRNDMYRGRSAERFYDALEWVFS
                     APEYVRNFAAGAAARSRRSSAPRRAASRAEARSTGSRARAARPPLRPPSRRRASPAR"
     gene            complement(1777..2583)
                     /locus_tag="VFX94_05945"
     CDS             complement(1777..2583)
                     /locus_tag="VFX94_05945"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5767758.1"
                     /translation="MAEKVFRNYDQKALDEQYEQRVWVPHADQIIKRYAERSDAVRKR
                     LGEPRVERYGPTPPETLDIYGSGKKAFVFVHGGAWKRQSSREQGFLAEPVVQAGAAYV
                     ALNFANLPTVTMSEMVGQVCRAIEWVHDHLCEDVVLCGHSSGGHLAACALIKKNLVRK
                     AMVVSGIYDLLPVRLSARNDYVRLDEPLEQEYSPIRHIHRIQCRVTVGWAEKESAEFL
                     RQSAEFAAALRAAGKLAESPEGKGLNHFDVLETMADPRSPLGRAALNMLA"
     gene            complement(2540..>2743)
                     /locus_tag="VFX94_05950"
     CDS             complement(2540..>2743)
                     /locus_tag="VFX94_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEX5767759.1"
                     /translation="ASQLLLHMAVAFAVMYGFTGSLAFGGIAAVVEPLCNVLLLPLHD
                     RLWRRIARRRGRKGLSQLRPEGA"
BASE COUNT          439 a          920 c          919 g          465 t
ORIGIN      
        1 cgcgacgtgg tcttcaagcc gtcctgggcg ctcgacatga tgcgcaacgg gttgccgggc
       61 atggcgaacc tcgccggcta tccgttcagc acgccgagcg gcaccgacat cgtcaccgcg
      121 gtcgggcgcg agatggacgc cggcctcgac tgggaataca tccagcgcct gcgcgagcag
      181 tggcccggga agctgctcct gaagggcgtg gagcgcgccg acgacgccga gcgggctgtc
      241 gcggtcggct gcgacggggt agtggtgtcg aaccacggtg gccgccaact cgacggcgca
      301 gcgcctacgc ttgaagcctt gcccgcgatt tcttccgcgg tcgggaacaa aatttccgtc
      361 ctgctggacg gcggggtccg ccgcggcgtc gacatcctga aagcgcgcgc cctgggcgcg
      421 caggcggtgc ttaccgggcg ggcgacgctc ttcggcgcga tggcggccgg agaggcgggc
      481 gcgctgcgcg cgctcgagat cctgtcgagc gagcttcagc gcgcgatgca gctctgcggg
      541 gcgcgctcga tcgccgagat cgggccgcag ctcctgcagc gaaattcctg acgtactcgg
      601 gcgcgctgaa cacccattcg agcgcatcgt agaaacgctc ggcgctacgt ccgcggtaca
      661 tgtcgttgcg caccagacgc tcgaggccgt cggggacgtg gaagatcatg ccgacaagcc
      721 ggttcgccga gacctgcacg cgggtcgcgc gcaccatgcg gcgtttctga tactcgagga
      781 acacgctttc gaagtctccg tcgagctctt cggcgcaaag cccgagacac accgcgtcct
      841 ccatcgccat cgccgcgccc tgcgccatgt actgaagcat gaagtgcgcc gcatcgccga
      901 ggagcgtgac gcgcccgcgg ctccagttgt ccaccggctc gcggtgcagc agcatccagc
      961 gccggaactc cttcggcgtg cgcatcagct tgagcggctt gtcgcagtag tgcgagaaga
     1021 acggcagcac ctcttcgggg tgcgcgagct cgttgtggcc gccgctcgta tgcttgccga
     1081 tgatcgtcgc gaccaggttg aagagcttcc agccccgcag cgggtagtgg acgatgtgcg
     1141 cgttgtgcgc ggcccacagc gtcgcggcgg cgaagcgcag atccttcggc acatcgttga
     1201 tatcgagcac tgcgcggtag cacatgtgcc cggtgatgag cggcagcgtg tcgcccacga
     1261 tgcgctcgcg gatcacggat ttgccgccgt ccgcgccgac gagggcaatg ccgcgaatcg
     1321 cgtctttctc cgttcttacc gtgacgcccg ttccttcatc ttcaaaaccg gtgacccggg
     1381 tacttgtccg tagctcgatg agatcgggca gtgccttgca gccgtcgagc agcgagccgt
     1441 ggatgtcggc acggtgagtg accgcgtagg ggttgccgaa gcgcttcgcg aagcgcctgt
     1501 cgagggggat gtcgatcacc ttctcgccgc tcaccccgtc gaacatgagg agatgctcga
     1561 tgaacaccgc ttccttcttc accagcgcgc caaccccgag cgcgtcgagc gcgtgccagg
     1621 cattgggcgc gatctggatc cccgcgccga tctcgccgaa ctgcgaggcc tgctcgagca
     1681 ccaccgagcg gaagcccttg cgcgccagcg cgagcgcggc cgcgagcccg ccgatgccgc
     1741 cgccggcgac gatgacgggc tcggcgttgc gcggtctcat gccagcatgt taagcgctgc
     1801 gcggccgagc ggggagcggg ggtcggccat cgtttcgagg acgtcgaagt ggttgaggcc
     1861 ctttccttcc ggcgactcgg cgagcttgcc ggccgctctc agcgcggcgg cgaattccgc
     1921 ggattgccgc aggaattcgg cggattcctt ctcagcccac ccgacggtta cgcggcactg
     1981 gatcctatgg atatggcgga tcggcgagta ctcctgctcc aggggctcgt ctaaccgcac
     2041 atagtcgttg cgcgcactaa ggcgcaccgg cagcaggtca tagatgccgg agacgaccat
     2101 ggcttttctg acgaggttct tctttatcaa agcgcacgcg gccaggtggc ctcccgagga
     2161 gtggccgcaa agcaccacgt cctcgcagag gtgatcgtga acccactcga tcgcccggca
     2221 tacctgccca accatctcgg acatcgtcac ggtgggcagg ttggcgaagt tgagcgccac
     2281 ataggccgcg ccggcctgca caaccggctc ggcgaggaat ccctgctcgc ggctcgactg
     2341 gcgcttccac gctccgccgt gcacgaagac gaacgccttc tttcccgagc catagatgtc
     2401 gagcgtctcg ggcggcgtcg gtccgtagcg ctccacgcgc ggctctccga gccgcttcct
     2461 tacggcgtcg cttcgctccg cgtagcgctt gatgatctgg tcggcgtgcg gcacccagac
     2521 gcgctgctcg tactgctcgt caagcgcctt ctggtcgtag ttgcgaaaga ccttttctgc
     2581 cacggcgacg ggctatgcgc cgccagaggc ggtcgtgcag cggaagcagc aatacgttgc
     2641 acagcggctc caccaccgcc gcgatgccgc cgaaggcgag cgagcccgtg aagccgtaca
     2701 tgaccgcgaa ggccaccgcc atgtggagga gcaactggct cgc
//
LOCUS       DASUNT010000296         2013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1003993, whole genome shotgun sequence.
ACCESSION   DASUNT010000296 DASUNT010000000
VERSION     DASUNT010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003993"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VFX94_05955"
     CDS             complement(<1..510)
                     /locus_tag="VFX94_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767760.1"
                     /translation="MRVAFILKGYPRLSETFIAQEIAALERRGLDILIVSLRRPTDSR
                     RHPVHEEIRAAVLYLPEYLLLEPLRVLRAWWNVRRSFNFKMVFSLWLKDLRRDLTPNR
                     MRRFGQALVLAAELPADVRHLHAHFLHTPASVTRYAAALLGLPWSGSAHAKDIWTTPD
                     WEKREKLASC"
     gene            complement(529..1749)
                     /locus_tag="VFX94_05960"
     CDS             complement(529..1749)
                     /locus_tag="VFX94_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767761.1"
                     /translation="MRSRRNDGRILIYSHDTFGLGHLRRCRTIAHSLVESFKQLSVLI
                     LSGSPIIGSFDFRTRVDFVRVPGVIKLRNGEYTSLNLHLDIRETMSMRASIIQHTAEK
                     FAPDVFIVDKEPVGLRGELLQTLKVLKRRGTRIILGLRDVMDEPRILEPEWQRKKVLP
                     ALREFYDEIWVYGLPQICDPLEGISLPTSVRKKMVYTGYLQREVPTHGAPPRLPEIVN
                     RPFLLVTTGGGGDGEGLIDWVLRAYEHDPLLPYPALLVLGPFMHADRQTEFMDRAAKL
                     KRVDAITFHGHLEALLARAVGVVAMGGYNTFCEVLSLDKRALIVPRTEPRLEQFIRAS
                     RAAELGLVSMLTDDRTYDPAVMAAALRALPRQNRPSEVVVPGLLEGLKNVSRLVAPWI
                     EPAELEARPISRIG"
     gene            complement(1766..>2013)
                     /locus_tag="VFX94_05965"
     CDS             complement(1766..>2013)
                     /locus_tag="VFX94_05965"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HEX5767762.1"
                     /translation="VFQVMYECYRARKLGEPNVEWLFPVYDTPKAELIAALPQALLDL
                     TWSCRRPLDGFRPCGVCKACMARQGLRVPAAPAENRA"
BASE COUNT          353 a          647 c          695 g          318 t
ORIGIN      
        1 gcatgaagcg agcttctcgc gcttttccca atcgggcgtg gtccagatgt ccttcgcgtg
       61 cgccgagccg ctccacggca gaccgaggag cgccgcggcg tagcgcgtga ccgaggccgg
      121 cgtgtgcagg aaatgcgcat gcaggtgcct tacatccgcc ggcagctcgg cggcgagcac
      181 cagcgcctga ccgaagcggc gcatgcgatt cggtgtcaag tcgcgtcgca agtctttcaa
      241 ccaaagagag aagaccattt tgaagttaaa gctgcgtcgt acgttccacc acgcacgcag
      301 gacgcgtaac ggctcgagga gcaggtactc gggcaggtag aggaccgcgg cgcggatctc
      361 ctcgtgcacg gggtggcgcc gggagtcggt cggccggcga agcgagacga tcaggatatc
      421 gaggccacgg cgctcgagcg cggcgatctc ctgcgcgata aacgtctcgg agaggcgcgg
      481 gtaccctttg agtatgaaag cgacccgcaa cccgcggcct tccgttgcct atccgatgcg
      541 cgagatgggg cgcgcctcga gctccgccgg ctcgatccac ggcgcaacca gccgcgagac
      601 gttcttgagg ccttcgagga gcccgggcac gacgacttcg gaaggccggt tctgccgcgg
      661 cagcgcgcgc agtgcggcgg ccatcaccgc tgggtcgtag gtgcggtcgt cggtgagcat
      721 gctgaccagg ccgagctcgg cggcgcggga agcgcggatg aattgctcga gccgcggctc
      781 ggtgcgcggc acgatgagcg cgcgcttgtc gagcgacagc acttcgcaga aggtgttgta
      841 gccgcccatg gcaaccacgc cgaccgcacg cgccaggagc gcctcgagat ggccgtgaaa
      901 agtgatcgcg tccacgcgct tgagcttggc cgcgcggtcc atgaactcgg tctggcggtc
      961 cgcgtgcatg aacgggccga gcaccagcag cgccgggtac ggcagcagcg ggtcgtgctc
     1021 gtaggcgcgc agcacccagt cgatcagccc ctcgccgtcg ccgccgccgc ccgtggtgac
     1081 gagcaggaac gggcggttga cgatctcggg aaggcgcggc ggcgcgccgt gcgtcgggac
     1141 ctcgcgctgc aggtagccgg tgtagaccat cttcttgcgc acgctcgtcg gcagcgagat
     1201 tccttcgagc ggatcgcaga tctgcggcag gccgtacacc cagatctcgt cgtagaactc
     1261 gcgcagggcg ggcagcactt tcttgcgctg ccactcgggc tcgaggatcc gcggctcatc
     1321 catcacgtcg cgcagcccaa ggatgatgcg cgtgccgcgg cgcttgagca ctttcagcgt
     1381 ctgcagcagc tcgccgcgca acccgaccgg ctccttgtcg acgatgaaga cgtcgggcgc
     1441 gaacttttcc gcggtgtgct ggatgatcga ggcacgcatc gacatcgtct cgcgaatatc
     1501 gaggtgcagg ttgagcgagg tgtactcgcc gttgcgcagc ttgatcacgc cgggaacgcg
     1561 cacgaaatcg acgcgcgtgc ggaaatcgaa gctgccgatg atcggcgagc ccgagaggat
     1621 caggaccgag agctgcttga agctctcgac gagcgaatgc gcgatcgtcc ggcagcggcg
     1681 caggtgcccg aggccaaacg tgtcgtggct gtagatgagt atgcggccat cgttccgcct
     1741 ggacctcatg gcgctcctcc ccctgtcaag cccgattttc ggctggcgcc gcgggaacgc
     1801 gcaagccctg ccttgccatg caggccttgc agaccccgca cggacgaaag ccgtccaagg
     1861 gccgccgaca ggaccatgtc aggtccagca gcgcttgcgg cagcgccgca atgagctcgg
     1921 ctttgggcgt gtcataaacc ggaaagagcc actcgacgtt cggctcgccc agcttgcgcg
     1981 cgcggtagca ctcgtacatg acttggaaaa cac
//
LOCUS       DASUNT010000297         2268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_741254, whole genome shotgun sequence.
ACCESSION   DASUNT010000297 DASUNT010000000
VERSION     DASUNT010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741254"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VFX94_05970"
     CDS             complement(<1..508)
                     /locus_tag="VFX94_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5767763.1"
                     /translation="MFSTIEDLAGRVPDGARVALAPDYSGCALAAVRALIRRRVQGLH
                     LIGVPQFGFQADLLIGAGCVASVEAAAVTLGEQGSAPRFVAAVKAGAIEIRDSTCPAL
                     HAGLLAAEKGVPFMPLRGVLGSDLVRHRPDWRVIDNPFAEEDPILLIPAIRPDVALFH
                     AARADRAGN"
     gene            945..>2268
                     /gene="hpnR"
                     /locus_tag="VFX94_05975"
     CDS             945..>2268
                     /gene="hpnR"
                     /locus_tag="VFX94_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008169 - C-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid C-3 methylase HpnR"
                     /protein_id="HEX5767764.1"
                     /translation="MRLLAVHPGPLMYTKIFLRLEPLGLEVVAQAARRAGHEVRLLDL
                     QVESQDRYLRTIGDWRPHAVAFSCNYLANIPEIVDLAKETKRRWPDIFVFVGGHSVSF
                     TAAAILEHAEGAIDCVLKGEGEASVADVLRAAEHDWAAVLRIPGVVARDGEGPRARFV
                     ETLDDAWPARDLLRHRRRYFIGTLDPCASIEFSRGCPWDCSFCSAWTFYGRSYRTVSP
                     ERAVEDLARIKEPGVFIVDDVAFIQDRHGLAIGEAIARKGIKKKYYLETRGDVLLRNK
                     EVFKFWKDLGVEYMFLGLEAIDEEGLKKYRKRVPLGTNFEALEFARSLGIAVAINIIA
                     DPDWDEERFRVVRAWCLEVPEIVNLSVNTPYPGTESWLSEARRLATRDYRLFDIQHAV
                     LPTRLPLDRFYRELVETQRVLNMKHLGARRLWQASGSIARALLRGQTNF"
BASE COUNT          377 a          750 c          774 g          367 t
ORIGIN      
        1 cgttgccggc gcgatcggcg cgcgcggcat ggaacaacgc cacatcgggc cggatggcgg
       61 gaatgaggag gatcggatcc tcctctgcga acgggttgtc gatgacgcgc cagtccgggc
      121 ggtggcgcac caagtcagag ccgagcacgc cgcgcagcgg catgaacggc acgcccttct
      181 cggccgccag caggcccgcg tgcagcgcgg ggcaggtgga atccctgatc tcgattgctc
      241 ctgccttgac cgccgcgacg aagcgcgggg ccgaaccttg ctcgccgagc gtcaccgcgg
      301 cggcctccac gctggcgacg cagcccgcgc cgatcagcag gtccgcctgg aagccgaact
      361 gcggcacgcc gatgaggtgc agcccctgca cgcgcctgcg gatcagcgcg cgtaccgccg
      421 cgagcgcgca gcccgagtag tcgggagcga gcgcgaccct cgcgccgtcc ggcacgcgtc
      481 ccgccaggtc ctcgattgtg gaaaacatcg gtactccaaa gcccgacgca gattgctcag
      541 cagcgcggcg cgaagccgac cataccgccg tcattataga gtggcccggc ccgccgtcac
      601 gccgctccct gcacagtgca acctcggcag gcttgtcgcg ggcgtgccga tcgaacaggg
      661 ggcaatcacg gcatggctct cggcgctcgc ggcagggtcg ctgatcgtct ggctacctcc
      721 tgctcggacg ggacggcttc gggcgcgcgc cccgcgaatc gaggacgagg tgccactgga
      781 gcgcggttcc tagccggccg tgagcctgac agagcatgat tgcctcgccc tgggcgcggc
      841 ctacaatggt tgggcaagcc ttcattctga gtttgcaacc cttaggtgcc cgaggggtgg
      901 cgctctgacc ggttggccta ctcgagtaag gggggacaag cgacatgcga ttgctggccg
      961 tgcatcccgg cccgctcatg tacaccaaga tcttcctccg cctggagccg ctcggtctgg
     1021 aggtggtcgc gcaggcggcg cgtcgggccg ggcatgaggt ccggttgctc gatcttcagg
     1081 tcgagagcca ggatcgttac ctgaggacga tcggcgactg gcggccccac gcggtcgcgt
     1141 tttcgtgcaa ttacctcgcc aacatcccgg agatcgtcga cctggccaag gagaccaagc
     1201 gacggtggcc ggacatcttt gtgtttgtcg gcggacacag cgtctcgttc acggcggccg
     1261 cgattctcga gcacgccgag ggcgccatcg actgcgtcct gaagggggaa ggcgaagcca
     1321 gcgtcgccga tgtgctccgc gccgccgagc acgactgggc ggccgtactc cgcatccccg
     1381 gggtcgtggc gcgcgatggc gaagggccgc gggcgcgctt cgtcgagacg ctggacgacg
     1441 cgtggccggc gcgcgatctg ctccgtcatc gccgcaggta cttcatcggc acgctcgatc
     1501 cgtgcgcctc gatagagttc tcgcgcggct gcccctggga ttgctcgttc tgcagcgcct
     1561 ggacttttta cggccgcagc taccgcacgg tcagccccga gagggcggtc gaggacctgg
     1621 ctcgcatcaa ggagcccggc gtgttcatcg tcgatgacgt ggcgttcatc caggaccggc
     1681 acgggctcgc catcggcgag gcaatcgcgc gcaaaggcat caagaagaaa tactacctgg
     1741 aaacgcgcgg tgatgtcctg ctgcgcaata aggaagtatt caagttctgg aaggacctag
     1801 gcgtcgagta catgttcttg ggccttgagg cgatcgacga ggagggactc aagaaatacc
     1861 gcaagcgcgt gccgctcggc accaatttcg aggcgctgga gttcgcccgc tcgctgggca
     1921 tcgcggtggc gatcaacatc atcgccgatc cggattggga cgaggagcgc ttccgggtgg
     1981 tgcgcgcgtg gtgcctggaa gtgccggaaa tcgtcaacct cagcgtcaac acaccctatc
     2041 ccggcaccga gagctggctg agcgaggcgc gcaggctcgc gacgcgcgac taccgattgt
     2101 tcgacatcca gcacgcggtg ctgccgaccc ggctgccgct cgatcgcttc tatcgcgagt
     2161 tggtcgaaac ccagcgcgtg ctcaacatga agcacctcgg cgcgcgcagg ctctggcaag
     2221 ctagcgggag catcgctcgg gctttgctcc gcggccagac caacttcc
//
LOCUS       DASUNT010000298         4819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_128752, whole genome shotgun sequence.
ACCESSION   DASUNT010000298 DASUNT010000000
VERSION     DASUNT010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128752"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VFX94_05980"
     CDS             <1..283
                     /locus_tag="VFX94_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEX5767765.1"
                     /translation="VADRATLDKQLDCLAALHGREEIAAWKSLAARGAWRDFVARLLV
                     EHYDPAYRRSSTRNFTQLVHATRVGVAWADDASFATAARSLLALTTATA"
     gene            617..1312
                     /locus_tag="VFX94_05985"
     CDS             617..1312
                     /locus_tag="VFX94_05985"
                     /inference="COORDINATES: protein motif:HMM:NF026037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX5767766.1"
                     /translation="MRPAQLHKVVLGTVICLAWAGQASAGDISGAIVYKGGAAARAKL
                     PVTIDQYICGKEKDAEDLVLSDKGGVGNAVVWIENPPNGTEWGKPLPKVEMDQKGCVF
                     VPRVVVVPVGGTVDFLNSDRLLHNVHSKSKDNATFNRTQPHGRVIPITFNKPEIVTIT
                     CDLHSWMRSWVVVAEHPFYAVTDPSGAFRIQNVPPGKYRLRMWQEALGSTSREVAVGA
                     QGASVTLDIKAPQ"
     gene            complement(1296..1715)
                     /locus_tag="VFX94_05990"
     CDS             complement(1296..1715)
                     /locus_tag="VFX94_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HEX5767767.1"
                     /translation="MAKIGRCGALLTALAFSPVAWAGQEEQFTPEFREVRASYDAKHT
                     PALTAPASVKRGSWFDVSVAVGAGGEHPSLSEHHIRYIALYKDSAEIARIYLHPVFSA
                     PKVTFTIALDESGTLRALAEPTHSAAWEASKKITVVP"
     gene            complement(1734..2108)
                     /locus_tag="VFX94_05995"
     CDS             complement(1734..2108)
                     /locus_tag="VFX94_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5767768.1"
                     /translation="MSQKTILYVEDNEFNRKIVKQLLAPTKYRLREATDGEAGLRMAH
                     EEAPDLILMDVQLPKMSGLEATRRLRADPQTAAIPIVVITSYALSGDAEKAKDAGATA
                     YLAKPYSPRDLLAKIREIVPEE"
     gene            complement(2105..3715)
                     /locus_tag="VFX94_06000"
     CDS             complement(2105..3715)
                     /locus_tag="VFX94_06000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5767769.1"
                     /translation="MRFDRPDPLARMLLRSPLGTLVRWVASARASVQVKLLAAFLLIT
                     LLFIAMGASSLYTVRSMSRQSELLDKAHQRVEWAQQIHHSLAMQMHLTAMALLVRDEA
                     ALGPILRENNRFSEALAHLEQAVEAPERAVIQKIRSGQEEAMITVADIANLVRDGKFD
                     AANKLHSGREDPLYRSIDALAYELVEKENARMVALRSSIQAANQRSLVVLGSFAAVSV
                     LLALAGGFIISWALIIPVHRAHDFLSELATGRFGESVTVENRDEFGELAAQLTATSKE
                     LARLDAVQRRAAQELGTLNARLEQASRAKSEFLANMSHELRTPLNAILGFTEMLSDGL
                     YGEMPRQFREPLADIQTNGKHLLRLINDVLDLSKIEAGKMELAVEEYSAQEVIQTVRS
                     SLRPLAEEKGLQFSAVVDESLPAVLYGDAKRITQCLLNLAGNALKFTKQGGVTIRAAR
                     EGDLIHYSVADTGIGIPEDQLDSLFTEFKQLNATITREFGGSGLGLSITKKFVEMHGG
                     RVWVQSRLNEGSTFHFTIPLKTEAVATA"
     gene            3745..>4819
                     /locus_tag="VFX94_06005"
     CDS             3745..>4819
                     /locus_tag="VFX94_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5767770.1"
                     /translation="MRTPPRIMVVDDTPANVRVLEARLAREGYEVIAAHDGEQALNAA
                     REQQPDLILLDIMMPKLDGIEVCRRLKGDPSFPFTPIIMITALSDAKDVVAGLEAGGD
                     DYLAKPVDQQALSARVKSMLRIKSLHDTVQEQAQRLKAQSAELERWNATLEQRVQQQL
                     AELEKIGRLKRFFSPQLAECIVQGGADDPLQSHRREVTVVFLDLRGYTSFAEVAEPEE
                     VMGVLREYHASMGELALAHEGTIERFTGDGMMIFFNDPVPIPNPAERALRMTVAMRDR
                     MEQLAARWRQRGYELNAGIGIAQGYATIGAIGFEGRLDYGAIGTVTNLAARLCGEAKP
                     GQILISRKLHGRVEDLVVAESVGE"
BASE COUNT          814 a         1572 c         1582 g          851 t
ORIGIN      
        1 cgtggccgac cgcgcaacgc tcgacaagca gctcgattgc ctggcggcgc tgcacggacg
       61 cgaggagatc gccgcgtgga aatcccttgc cgcgcgggga gcctggcggg atttcgtcgc
      121 gcgcctcctg gtcgagcact acgaccccgc ataccgccgc tcctctaccc gcaacttcac
      181 ccagctcgtt cacgccacgc gcgtcggcgt cgcctgggcc gacgacgcaa gcttcgccac
      241 cgccgcgcgc tcgctcctcg cgctcaccac tgccaccgcc taggcccggc atgcggcgga
      301 tgcaggagtt cggcatcgac gccacgcaga gaagacgttc gtcgtcatcg ccgacggaag
      361 cgcctacgta aagtctgacg ccgcgattcg tgtcgcgcgc ttgctctcct tcgtcgcacg
      421 caaccggtat cgctggttcg ggggccacgc agcctgcatc gaacctacat cggaactgca
      481 aagcgtttct atagccggcc cggcaatgcc tcgagcgccg tagtggccgc ggccacattt
      541 gtggttggga gatcgacgct tctctgcacg ccggacagcc ctggaccttg ctatatttcg
      601 cgcacgcgga ggagagatga gacctgcgca gttgcacaaa gtggtccttg gtacagtgat
      661 ctgtcttgcc tgggcaggac aagcgagcgc cggggatatc agcggcgcca tagtctacaa
      721 gggtggcgct gcggcgcgtg ccaagctgcc agtcacgatc gaccagtaca tctgcggaaa
      781 ggagaaggac gcagaggatc tcgtgctgag cgacaagggc ggggtcggga acgcggtggt
      841 ctggatcgag aatccgccca acggcaccga atggggaaag ccgctgccca aggtcgagat
      901 ggaccagaaa ggatgcgtat tcgtcccgcg ggtggttgtc gtcccggttg gcggcacggt
      961 ggactttctc aacagcgacc gattgctcca caacgtgcac agcaagagca aggacaacgc
     1021 gacgttcaac cgcacccagc cgcatggacg cgtcattccg atcacgttca acaagccgga
     1081 gatagtcacg atcacctgcg acctgcactc gtggatgcgc agctgggtcg tggtggcgga
     1141 gcacccgttc tatgcggtga ccgacccgag cggcgctttc aggattcaga acgtgccgcc
     1201 gggcaaatac cgcctgcgca tgtggcagga agcgctcggc agcacgtcgc gcgaagtggc
     1261 cgtcggcgcg cagggcgcga gcgtgacgtt ggacatcaag gcaccacagt aatcttcttc
     1321 gaggcctccc atgccgcgga gtgcgtcggc tcggcgagcg cacgcagcgt gccgctttcg
     1381 tccaaggcga tggtgaacgt caccttcggc gccgaaaaca cggggtgaag gtagatgcgc
     1441 gcgatctcag cgctgtcctt gtagagcgcg atgtatcgga tgtgatgctc ggagagcgag
     1501 ggatgttcgc cgccggcgcc gacggccacg ctgacgtcga accagctgcc acgctttacc
     1561 gatgccggcg cggtcagggc cggggtgtgc ttcgcgtcat aggaagcccg cacctccctg
     1621 aattccggcg tgaactgctc ttcctgcccg gcccacgcca cgggcgagaa cgccagcgcg
     1681 gtgagcaacg cgccgcacct tccgatcttt gccatgtctt gcctctctcg atgtcactcc
     1741 tcgggaacga tctcccggat ctttgccagc aaatctctcg gactgtacgg cttggcaagg
     1801 tacgccgtcg ctccggcgtc cttcgctttc tcggcatctc cgctgagcgc ataggacgtg
     1861 atcacgacga tcgggatcgc cgccgtttgc ggatccgcgc gcagccggcg ggtcgcctcc
     1921 agcccggaca tcttcggcag ctgcacgtcc atcaggatca ggtccggcgc ctcctcgtgc
     1981 gccatgcgca ggcctgcctc gccgtcggtc gcctcgcgca gccggtactt cgtcggcgca
     2041 aggagctgct tgacgatctt gcgattgaac tcgttgtctt cgacgtagag aatggttttc
     2101 tggctcatgc ggtagccacc gcctccgtct tgagcgggat cgtgaagtga aaagtcgagc
     2161 cttcgttcag ccggctttgc acccagacgc gtccgccgtg catttccacg aactttttcg
     2221 tgatgctgag cccaaggccg ctgccgccga actcgcgcgt gatcgtcgcg ttcagctgct
     2281 tgaattccgt gaacagggaa tcgagctggt cctcggggat tccaattccg gtatcggcaa
     2341 cgctgtaatg gatgagatca ccctcgcgcg cggcgcgaat ggtgacgccg ccctgcttgg
     2401 tgaacttgag cgcattgccg gcaaggttca gcaggcactg ggtgatgcgc ttcgcgtcgc
     2461 cgtacaagac tgcgggcagg ctctcgtcca cgacggcgct gaactgcaaa cctttctcct
     2521 cggcaagcgg acgcagcgac gagcgcacgg tctggatcac ctcttgcgcg gagtattcct
     2581 ccaccgcgag ctccattttc ccggcctcga tcttggagag atcgagcaca tcgttgatca
     2641 ggcgcagaag atgcttgccg ttggtctgaa tgtcggccag cggctcccgg aattgccgcg
     2701 gcatctcgcc gtagagtccg tcgctcagca tctccgtgaa gccgaggatc gcgttcagag
     2761 gcgtgcgcag ctcgtggctc atgttggcga ggaactcgga cttcgcccgg ctcgcctgct
     2821 cgagcctcgc gttgagcgtg ccgagctcct gcgcggcgcg ccgctgcacg gcgtcgaggc
     2881 gcgcgagctc cttgctggtc gcggtaagtt gcgccgcaag ctcgccgaat tcatcgcgat
     2941 tctcgaccgt gacgctttcc ccgaagcgtc cggtggccag ctcgctgagg aagtcgtgcg
     3001 cgcggtggac ggggatgatg agcgcccacg agatgatgaa gccgccggcg agcgcgagca
     3061 gcaccgagac cgcggcgaag ctgcccagga cgacgagcga gcgctggttg gccgcctgga
     3121 tgctgctgcg cagcgccacc atgcgcgcgt tctccttctc gacgagctcg taggcgagcg
     3181 cgtcgatcga gcggtacagg ggatcctcgc gcccgctgtg cagcttgttc gccgcatcga
     3241 acttgccgtc gcgcaccagg ttcgcgatgt ccgcgacggt gatcatcgct tcctcctgcc
     3301 cgctgcggat cttctggatc accgcccgct ccggcgcttc cactgcctgc tccaggtgcg
     3361 ccagcgcttc gctgaaccgg ttgttctcgc gcaggatggg gccgagcgcc gcttcgtcgc
     3421 gcaccagaag cgccatggcg gtgaggtgca tctgcatcgc cagcgagtgg tggatttgct
     3481 gcgcccattc gacgcgctga tgcgccttgt cgaggagctc gctttggcgc gacatgctgc
     3541 gcacggtgta caggctggac gcgcccatgg cgatgaacag cagcgtgatc agcaggaaag
     3601 cggcgagaag cttgacttgt accgaagcgc gtgcgctcgc tacccagcga accagcgttc
     3661 cgaggggact gcgcagcagc atccttgcga gcggatccgg tcgatcgaac cgcatggggg
     3721 cgtcggcgag tatactccgg cagcttgagg acgcccccgc gcatcatggt ggtggacgat
     3781 acgcccgcga atgtgcgcgt gctcgaggcg cggctggcac gggagggcta cgaggtgatc
     3841 gccgcgcacg acggcgagca ggcgctcaac gcggcgcgcg agcagcagcc cgatctcatc
     3901 ctactcgaca tcatgatgcc gaagctcgac ggcatcgagg tatgccgcag gctgaagggc
     3961 gatccctcct tcccgtttac gccgatcatc atgatcaccg cgctgtccga cgcgaaagac
     4021 gtggtggccg ggctggaagc gggcggcgac gactatctcg cgaagcccgt ggatcagcag
     4081 gccctttcgg cgcgcgtcaa atcgatgctg cgcatcaagt cgctccacga caccgtgcag
     4141 gagcaggcgc agcgcctgaa agcgcagtcg gctgagctcg agcgctggaa cgcgacgctc
     4201 gagcagcgcg tccagcagca gctcgcagag ctggagaaaa tcggccggct caagcgcttt
     4261 ttctcccctc agcttgccga gtgcatcgtc cagggcggcg ccgacgatcc gctgcagtcg
     4321 caccggcgcg aggtcacagt cgtgtttctc gacctgcgcg gctacacctc gttcgccgag
     4381 gtcgccgagc ccgaagaagt gatgggcgtg ctgcgcgaat accatgcgtc gatgggcgag
     4441 ctggcgctgg cgcacgaagg caccatcgag cgcttcaccg gcgacggcat gatgattttt
     4501 ttcaatgatc cggtgccgat tcccaacccg gccgagcgcg ccctgcgcat gaccgtggcc
     4561 atgcgcgatc gcatggagca gctggccgcg cgttggcgcc agcgcggcta cgagctgaac
     4621 gcgggcatcg gcatcgcgca aggttatgcc acgatcggcg ccatcggctt cgaggggcgc
     4681 ctggactacg gcgccatcgg cacggtgacc aacctcgccg cccgcctctg cggggaggcc
     4741 aagcccgggc agatcctcat ctcccgcaag ctccacgggc gggtggagga cctcgtggtg
     4801 gcggagtccg tgggcgagc
//
LOCUS       DASUNT010000299         2331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_479210, whole genome shotgun sequence.
ACCESSION   DASUNT010000299 DASUNT010000000
VERSION     DASUNT010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479210"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VFX94_06010"
     CDS             <1..282
                     /locus_tag="VFX94_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5767771.1"
                     /translation="IALTKTLAREMASKNIQLNVVCPGLTETNMLEQFMQGAGNPEKL
                     REAYRRAIPIGRLGKPEDLPGAILFFASDDADFITGQVLSVSGGLTMHG"
     gene            complement(333..1070)
                     /locus_tag="VFX94_06015"
     CDS             complement(333..1070)
                     /locus_tag="VFX94_06015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5767772.1"
                     /translation="MKGRIAVVTGGAGGMGRGAALKLAQEGAAIEILDRQDAAQACEA
                     IRQAGGAASAMACDTTDETQIARCARAIEDRHGRIDILVNNAGLLSGREPWHSLPRRE
                     LERFLQVNFVGYFLVAQAFYPLLKKSKAGRIINVASRTFFLANPGQMAYVASKGAVWG
                     MTRVLARECGDDNITVNAVAPGMIATPGTMAHSKEEAFERVMQNQAIKKRGKPEHLAN
                     LIAFLASDEAEMITGQFMLADGGGYLH"
     gene            complement(1078..1914)
                     /locus_tag="VFX94_06020"
     CDS             complement(1078..1914)
                     /locus_tag="VFX94_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase family protein"
                     /protein_id="HEX5767773.1"
                     /translation="MATIVGAITTSHVPAIGGAIARGLQDEPYWKPFFGAFGRARNWL
                     EEVKPDIAVVVYNDHGLQFFLDKMPTFAVGAADEYRNADEGWGLPTMPPYRGDAAFSW
                     HLIESLVADEFDVTSCQEVLVDHAFTVPMQLLWPSGCPVATVPVLVNTVQHPLPSPTR
                     CLKFGRALGRAITAWPGGERVLVVGTGGLSHQLDGARAGFINREFDQMCMTKIVEEPD
                     ALGAYSIPELVELAGTQGVELLNWLVARAALGGRVSRIHSNYHVPISNTASGMMVLEH
                     RS"
     gene            complement(1917..2258)
                     /locus_tag="VFX94_06025"
     CDS             complement(1917..2258)
                     /locus_tag="VFX94_06025"
                     /EC_number="1.13.11.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
                     /protein_id="HEX5767774.1"
                     /translation="MKRREIPGTTVFDGAQARKGYALNRMCYSFNDAANRAEFLRDED
                     AYCRRFGLSEAQRAAIRRRNVLELIAAGGNVYYLAKFAGIFRLDVQDIGAQQTGMTKE
                     AFKAMLAEKGK"
BASE COUNT          362 a          776 c          788 g          405 t
ORIGIN      
        1 attgccctca cgaaaacgct cgcgcgcgag atggcttcga agaatattca actgaacgtg
       61 gtgtgcccgg gcctcacgga aaccaacatg ctcgagcagt tcatgcaggg cgcgggcaac
      121 cccgagaagc tgcgcgaggc ctaccggcgc gcgatcccga tcggccgcct gggcaagccc
      181 gaggatctgc cgggagcgat tctcttcttc gcgagcgacg acgcggactt catcaccggc
      241 caggtcctga gcgtgtcagg cgggctgacg atgcacggct agaaagtctt ctggcgtcgt
      301 ccccgcgaag gcggggatcc cgtttatctc agtcagtgca ggtagccgcc gccgtcggcg
      361 agcatgaact ggccggtgat catctcggcc tcgtcgctcg cgaggaaggc gatcaggttg
      421 gcgaggtgct cgggcttgcc gcgcttcttg atcgcctggt tctgcatcac gcgctcaaac
      481 gcctcttcct tcgaatgggc catcgttccc ggcgtggcga tcatgcccgg cgccaccgcg
      541 ttcacggtga tgttgtcgtc gccgcactcg cgcgccagca cgcgcgtcat gccccatacc
      601 gcacccttgc tcgccacgta cgccatctgt ccggggttgg cgaggaagaa ggtgcgcgag
      661 gccacgttga tgatgcgccc cgccttgctc ttcttgagca gggggtagaa cgcctgggcg
      721 acgaggaagt agccgacgaa gttgacctgc aggaagcgct cgagctcgcg gcgcggcagg
      781 ctgtgccagg gctcgcgtcc ggacagcagg cccgcgttgt tcaccaggat gtcgatgcgc
      841 ccgtgccggt cctcgatggc gcgcgcgcag cgcgcgatct gggtctcgtc ggtcgtgtcg
      901 catgccatcg cgctcgcggc gccgccggcc tgccggatcg cttcgcaggc ctgcgccgca
      961 tcctgccggt cgaggatctc gatcgccgcg ccttcttgcg cgagcttgag cgcggcgccg
     1021 cggcccatgc cgcccgcgcc gccggtgacg acggcaatcc ttcccttcaa tcgcatttca
     1081 gctcctgtgc tcgagaacca tcatccccga cgcggtgttc gagatgggga cgtggtagtt
     1141 gctgtggatc cgggaaacgc ggccaccaag cgcggcgcgc gccaccagcc agttgaggag
     1201 ctccactccc tgcgtgccgg cgagctcaac gagctcgggg atcgagtagg caccgagcgc
     1261 atcgggctcc tcgacaatct tcgtcatgca catctgatcg aactcgcggt tgatgaagcc
     1321 agcgcgggcg ccgtcgagct ggtgcgagag gccgccggtg ccgacaacaa gaacgcgttc
     1381 gccgccgggc cacgccgtga tggcgcggcc gagggcgcgg ccgaacttca ggcagcgggt
     1441 cggcgagggc agcggatgct gcaccgtgtt caccagcacg gggaccgtgg cgaccgggca
     1501 accggacggc cacaagagct gcatcggcac ggtgaacgca tggtcgacca gcacctcctg
     1561 gcagctggtg acgtcgaatt cgtctgcgac tagcgactcg atcaggtgcc aggagaaagc
     1621 cgcgtcgccg cgatagggcg gcatcgtcgg caggccccat ccctcgtccg cgttgcggta
     1681 ttcgtccgcg gcgccgacgg cgaaggtcgg catcttgtcg agaaagaact gcaggccgtg
     1741 gtcgttgtag acgacgacgg caatgtctgg cttcacctcc tcgagccagt tgcgggcccg
     1801 gccgaacgcg ccaaagaacg gcttccagta gggctcgtcc tgcagccccc tcgcgattgc
     1861 gccgccgatc gcgggcacgt gcgaggtggt gatggcgccg acgatcgtgg ccacggctat
     1921 ttccctttct cagcgagcat cgccttgaag gcttctttcg tcatcccggt ctgctgcgcg
     1981 ccgatgtcct gcacgtcgag gcggaagatg ccggcaaact tcgcgaggta gtacacgttg
     2041 ccgcctgcag cgatcagctc cagcacattg cggcgccgga tcgccgcgcg ctgcgcctcg
     2101 ctcaggccga acctgcggca gtaggcgtcc tcgtcgcgca gaaactcggc gcggttggcc
     2161 gcgtcattga acgagtagca catgcggttc agggcgtagc ctttgcgcgc ctgcgcgccg
     2221 tcgaagacgg tggtgccggg gatctccctg cgcttcatgc cgccttgcgc cggatggcct
     2281 tgcgccgctc ggcctcggcg gtgaattctt ccaggtcggg atcgagcgcg t
//
LOCUS       DASUNT010000300         3098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_829935, whole genome shotgun sequence.
ACCESSION   DASUNT010000300 DASUNT010000000
VERSION     DASUNT010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829935"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..766
                     /locus_tag="VFX94_06030"
     CDS             89..766
                     /locus_tag="VFX94_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5767775.1"
                     /translation="MVELRVKDGIAGIFLNRPEKANALTSAALETLRAALRELAADPA
                     LRAVVLGGRGRNFCGGADRLELRSLTASTAGAFVEKIHHVCQAIRELPVPVVARLHGA
                     VIGAGLEIAAACDLRVAADGTKFAMPEVRLGIPSVVEAALLPRLMGSGRAAWLVLTGE
                     AIDARRALEWGLIEEVGDEQAVLKPLLAADGQALRMQKQLLQLWDEAPLATSVAASLE
                     RFARLHQ"
     gene            805..1062
                     /locus_tag="VFX94_06035"
     CDS             805..1062
                     /locus_tag="VFX94_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX5767776.1"
                     /translation="MITVGSFEAKTKLAELLDKVEAGETVLITRRGKAVAKLVPAAVD
                     AEERARRRAFIAEVKRKRVAWDRGAKPGAKIADLIKAGRRY"
     gene            1062..1520
                     /locus_tag="VFX94_06040"
     CDS             1062..1520
                     /locus_tag="VFX94_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5767777.1"
                     /translation="MAVVFDCSVTLAWFLEGERTAFTDTAFELLETEECWVPYLWRLE
                     FPNALLFAERKKRLGRAQRLEILDNAAQLFLRTDPALPDLRSLSALAERRGLTVYDAS
                     YLELAVRSGFDLITLDRELADAALAEGVSAHSPGRSSAARVRKRYAAEAA"
     gene            1566..>3098
                     /locus_tag="VFX94_06045"
     CDS             1566..>3098
                     /locus_tag="VFX94_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5767778.1"
                     /translation="MAKPQVYDASAIQALKGLEPVRRMPGMYTHTVHPLHIVQEAIDN
                     AVDEALAGFGKRITVSVFKDGSFQVEDEGRGIPVDMHPVEKKPAVEVAFTMLHAGGKF
                     SRSGDGVYHISGGLHGVGVSVSNALSKRCEVVVYRGNERHSIVFEGGEVKQKLRSEKT
                     REKRTGTIVRLWPDPKYFDSPHIPMNELEHLVRSKAYLLPGLTMALEVEGKQTRLWKY
                     ERGMAQYFEFMLDGRELVAPLFTGERFFDEKSLSGVTDIEPGEGASWAIGWVAEGEIF
                     ADSHVNLIPTRSGGTHEAGFRAGIFEALGAFMDGRGLAPKGVKLIADDLWQRACFTLS
                     ARIVRTQFHGQTKEKLTTRHAARLLELCVRDAFELWLNEHVDEGKRIVELAIEQAMAR
                     LAKGKKVERKKSSGIATLPGKLVDCASGDISRNELFLVEGDSAGGSAKEARDKETQAL
                     LPLRGKVLNSMSKDSRTVLANKELQAIATAIGVDPHSSEEKADLGGLRYGKVIVMTDA
                     DVD"
BASE COUNT          536 a          999 c         1086 g          477 t
ORIGIN      
        1 tgaagccgcg cttgaacagc accgtggtga gcgtagcggt ggagactttc ttcagcgctt
       61 cgcgcgcggc agcggataga cttggcatat ggtcgagttg agagttaagg acgggattgc
      121 gggcatcttc ctcaaccggc ccgaaaaagc aaacgccctg acgagcgccg cgctcgaaac
      181 gctgcgggcc gcgttgcggg aacttgcggc cgatccggcc ctcagagccg tggtcctcgg
      241 cggacgcggc cgcaacttct gcggcggcgc cgaccggctg gagctaagaa gcctcaccgc
      301 atcgaccgcg ggggcgtttg ttgaaaaaat ccaccacgtc tgccaggcga tccgggagct
      361 gccggtgccg gtggtggcgc ggctgcacgg cgcggtgatc ggcgccggcc tcgagatcgc
      421 tgccgcctgc gacctgcgcg tcgccgcgga cggcacgaag ttcgccatgc cggaagtgcg
      481 gctcggcatc ccctcggtag tcgaggcggc gcttttgccg cggctcatgg gctcgggtcg
      541 cgccgcctgg ctcgtgctca ccggtgaagc gatcgacgcc cggcgcgccc tggaatgggg
      601 cttgatcgag gaagtcggcg acgagcaggc cgttctgaag cccctgctcg ccgcagacgg
      661 ccaagcgttg aggatgcaaa aacagctgct gcagctctgg gacgaggcgc cgctcgcgac
      721 ctcggtggcg gcgtcgctcg agcgcttcgc gcggcttcat caataggagt atcctttcaa
      781 ctggttgtct agttggaggc aacgatgatc acggtcggct ccttcgaggc gaaaaccaag
      841 ctcgccgagc tgctcgacaa ggtcgaggcg ggcgaaacag tcctgattac ccgccgcggc
      901 aaggcggtgg cgaagctcgt cccggccgcg gtggacgcgg aggagcgcgc gcggcggcgc
      961 gcgttcatcg cggaggtcaa gcgcaagcgg gtggcctggg atcggggtgc gaagcccggc
     1021 gcgaagatcg ccgatctgat caaggccggc cgacgctact gatggcggtg gtcttcgact
     1081 gctccgtgac gctcgcctgg ttcctggaag gcgagcgcac cgcgtttacc gacaccgcgt
     1141 tcgagctgct cgagaccgag gaatgctggg taccgtacct ctggcgcctc gagttcccga
     1201 acgcgctgct cttcgccgag cgcaagaaac gcctcggccg cgcgcagcgg ctggagatcc
     1261 tggacaacgc cgcgcagctt tttctgcgca ccgacccggc gctgccggat ctgcgctcgc
     1321 tgagcgcgct ggcggagcgc cgcggcctca cggtctatga cgcatcctac ctggagctcg
     1381 ccgtgcgctc gggcttcgac ctcatcacgc tcgatcggga gctcgcagac gcggcgctcg
     1441 ccgaaggggt gagcgcgcat tcgcccgggc gctcgagcgc ggcccgggtg cggaaacgct
     1501 acgccgccga ggccgcctga gcgctaccat acctagtaga caacgagcta aactgcacta
     1561 gatccatggc caagccgcag gtttacgacg catccgcgat ccaggcgctg aagggcctcg
     1621 agccggtccg gcgcatgccg gggatgtaca cccacaccgt gcatccgctg cacatcgtcc
     1681 aggaggcgat cgacaacgcg gtcgacgagg cgctcgccgg cttcggcaag cgcatcacgg
     1741 tgagcgtgtt caaggacggc tcgttccagg tcgaggacga ggggcgcggc atcccggtcg
     1801 acatgcaccc ggtcgagaag aagcccgcgg tcgaggtggc gttcaccatg ctgcacgcgg
     1861 gcggcaagtt ctcgcgctcg ggcgacggcg tctaccacat ctccggcggc ctgcacgggg
     1921 tcggggtctc ggtgtcgaac gcgctttcga agcgatgcga agtggttgtt taccgaggta
     1981 acgaacgcca ttcgatcgtg ttcgaaggcg gtgaagtcaa gcagaagctg agatccgaga
     2041 agacccggga aaagcgcacc ggcacgatcg tgcggctgtg gcccgatccg aagtacttcg
     2101 actcgcccca catcccgatg aacgagctcg agcacctggt gcgctccaag gcgtacctgc
     2161 tgcccggcct gacgatggcg ctcgaagtcg agggcaagca aacccgcctg tggaaatacg
     2221 agcgcgggat ggcgcagtac ttcgagttca tgctcgacgg gcgcgagctc gtcgcgccgc
     2281 tcttcaccgg ggagcggttc ttcgacgaga aatcgctctc cggcgtcacc gatatcgagc
     2341 ccggcgaggg cgcttcgtgg gcgatcggct gggtcgccga gggcgagatc ttcgccgaca
     2401 gccacgtgaa cctcatcccg acgcgctcgg gcggcacgca cgaagcgggg tttcgcgccg
     2461 gcattttcga ggccctcggc gcgttcatgg acggacgcgg cctcgcgccg aagggcgtga
     2521 agctgatcgc ggacgatctc tggcagcgcg cctgcttcac gctctcggcg cgcatcgtgc
     2581 gcacgcagtt ccacggccag accaaggaga agctcaccac gcgccacgcc gcgcggctgc
     2641 tcgagctgtg cgtgcgcgac gcgttcgagc tctggctgaa cgagcacgtc gacgaaggca
     2701 agcgcatcgt cgagctcgcg atcgagcagg cgatggcgcg cctcgccaag gggaagaaag
     2761 tcgagcgcaa gaagtcgagc ggcatcgcca cgctcccggg aaagctcgtc gactgcgcct
     2821 cgggcgatat ctcgcgcaat gagctgttcc tggtggaagg cgactcggcg ggaggctcgg
     2881 cgaaggaggc gcgcgacaag gagacgcagg cgctgctgcc gctgcgcggc aaggtgctga
     2941 actcgatgtc gaaggacagc cgcaccgtgc tcgccaacaa ggagctgcag gcgatcgcga
     3001 ccgcgatcgg cgtcgatccg cattcctccg aagaaaaggc cgaccttggc ggcctgcgct
     3061 acgggaaggt catcgtgatg accgacgccg acgtcgac
//
LOCUS       DASUNT010000301         3191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_829981, whole genome shotgun sequence.
ACCESSION   DASUNT010000301 DASUNT010000000
VERSION     DASUNT010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829981"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VFX94_06050"
     CDS             complement(<1..590)
                     /locus_tag="VFX94_06050"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5767779.1"
                     /translation="MRFASLASGSSGNCLVAEAAGTVVLVDCGLALTETERRLARLGL
                     EPSRVRAILVTHEHADHADGAFEFAAAHRITLYLTHGTLAALKAEGKVLDGVPLALVN
                     GRQSFFVDGMQLLPFTVPHDAREPVQYVLSDGAARLGIITDVGISTAHVEKSLSGLDA
                     LVLECNYDHDMLWNGGYPRWLKERIAGPFGHLDNRES"
     gene            complement(598..1752)
                     /gene="bamC"
                     /locus_tag="VFX94_06055"
     CDS             complement(598..1752)
                     /gene="bamC"
                     /locus_tag="VFX94_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEX5767780.1"
                     /translation="MPKHFMLTAAAAAVLSGCSWFSSDSSSVDYKSAGSLPPLEVPPD
                     LTSPQRDNRYAIPEPARSSTTLSGYEAERRDPAARGARPTAASPSGPVVLPVIENVKV
                     ERAGAQRWLVVQEPPEQLWPVVKAFWQENGFLIRTELPEAGVMETDWAESRARIQEGA
                     VRDFLGKVFDYFHSTAERDKYRTRLDRTADGGTEIYISHRGMQELYTTRDPRGDTPGQ
                     TAWQPRPPDPELEAEFLRRLMVRLGTPGERAKQLTASSEPNQRAEIVKANDGTERLQV
                     HEPFDRAWRRVGLALDRVGFTVEDRDRQKGLYFVRYADPESEVAQKERGFMSRIFSFG
                     SDSKVKAEQYRVQVSSETSGSQVHVLNKDGAAESSKTAQRILALLHEQLK"
     gene            complement(1752..2660)
                     /gene="dapA"
                     /locus_tag="VFX94_06060"
     CDS             complement(1752..2660)
                     /gene="dapA"
                     /locus_tag="VFX94_06060"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX5767781.1"
                     /translation="MITGSLVAIVTPMHEDGRLDLGRFRQLIDWHAAEGTDGIVVVGT
                     TGESPTVDFDEHKELIRLAVQHSRGRIPVIAGTGGNSTAEAIELTESAKKNGATACLS
                     VVPYYNKPTQEGLYRHFRKIAETVDLPMIVYNVPGRTVADLANDTMLRLAQVPGIIGI
                     KDATANIERGTDLIKRAPRNFAIYSGDDATALALILLGGHGVISVTANVAPKLMHQMC
                     AAALVGDLKKARELNLRLFALHQRLFVEANPIPVKWALAEMGMIEGGMRLPMSPLSEK
                     FHQTVREALHEAGIALPGLRVVEGRG"
     gene            complement(2657..3181)
                     /locus_tag="VFX94_06065"
     CDS             complement(2657..3181)
                     /locus_tag="VFX94_06065"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5767782.1"
                     /translation="MAADPHDLRTPSPWVVRWAPLVKRGPVLDVASGAGRHARLFAAR
                     GLEVVAVDREVRVIPGVRFVKADLEDGSPWPFAGRRFGGIVVTNYLHRPIFGRLAAAL
                     EEGGALIYETFMLGNERFGKPSNPAFLLHPGELLEAFRVLTVVAFEQGESANAVRQRI
                     CVVRGDASRVRIPA"
BASE COUNT          492 a         1101 c         1068 g          530 t
ORIGIN      
        1 gactcgcggt tgtcgaggtg gccgaacggg ccggcgatgc gctccttgag ccagcgcggg
       61 tagccgccgt tccaaagcat gtcgtggtcg tagttgcact cgagcacgag tgcgtcgagc
      121 ccggagagcg atttctccac gtgcgcggtg gagatgccga catcggtgat gatgccgagc
      181 ctcgcggcgc cgtcggagag cacgtactgg acgggctcgc gcgcatcgtg gggcacggtg
      241 aagggcagca gctgcatgcc gtcgacgaaa aacgactggc ggccgttcac cagagcgagc
      301 ggcaccccgt cgagcacctt gccttccgcc ttcagcgcag ccagtgtgcc gtgggtaagg
      361 tagagcgtaa tccggtgcgc ggcggcgaac tcgaacgccc cgtcggcgtg gtcggcgtgc
      421 tcatgggtga cgaggattgc tcttacccgc gacggctcga gcccgagccg cgcgagccgc
      481 cgctcggtct cggtgagcgc cagcccgcag tccacgagca ccaccgtccc cgcggcctcg
      541 gctaccaggc agttgcccga gctgccgctg gcgagcgagg cgaagcgcaa gcgagcgcta
      601 cttcagctgc tcatggagca gcgcgagaat gcgctgcgcg gtcttcgagc tttcggcggc
      661 gccgtccttg ttcaggacgt gcacctggct gcccgaagtc tcggacgaaa cctgcacccg
      721 gtactgctcc gccttgacct tggagtcgct gccgaagctg aagatgcgcg acatgaaccc
      781 gcgctccttt tgcgccacct cggactcggg gtcggcgtag cgcacgaagt acaggccctt
      841 ctgccggtcg cggtcttcca cggtgaaacc gacgcggtcc agcgcgagcc cgacacgccg
      901 ccaggcgcgg tcgaaaggct cgtgcacctg caggcgctcg gtgccgtcgt tggctttcac
      961 gatctcggcg cgctggttgg gctcactgct cgccgtgagc tgcttggcgc gctcgccggg
     1021 cgtgccgagc ctgaccatca gccggcgcag gaattcggcc tcgagttccg ggtcgggcgg
     1081 ccgcggctgc cacgcggtct gccccggggt gtcgcctctg ggatcgcgcg tcgtgtagag
     1141 ctcctgcatg ccgcgatggc tgatgtagat ctcggtgccg ccgtccgcgg tgcgatccag
     1201 ccgcgtgcgg tacttgtcgc gctcggcggt ggagtgaaag tagtcgaaca ccttgccgag
     1261 aaagtcgcgc accgcgccct cctggatgcg cgcccggctc tccgcccagt cggtctccat
     1321 cacgccggcc tcgggaagct cggtcctgat gagaaaaccg ttctcctgcc agaacgcctt
     1381 caccacgggc cagagctgct cgggcggctc ctgcacgacg agccagcgct gcgcgcccgc
     1441 gcgttcgacc ttcacgttct cgatcacggg cagcaccacc ggcccgctcg ggctggcggc
     1501 cgtcggacgc gcgccgcgcg cagccgggtc gcggcgctcg gcctcgtagc ccgagagcgt
     1561 cgtgctggag cgcgcaggct ccggaattgc gtagcggttg tcgcgctgcg gggacgtgag
     1621 atcgggcggc acttcgagcg gcggcaggga tccggcggac ttgtaatcca ccgaggacga
     1681 gtcgctgctg aaccacgagc aaccagacag cacggccgcc gccgcggccg tgagcatgaa
     1741 atgctttggc atcaaccccg gccttctacg actcttaatc ccggcagcgc gatgccggct
     1801 tcatgcagcg cttcgcggac cgtttgatgg aatttctccg agagcggcga catcggcagc
     1861 cgcatgcctc cctcgatcat tcccatctcg gcgagcgccc acttgaccgg gatcggattc
     1921 gcttcgacga acaggcgctg gtgcagcgcg aacagcctga ggttcagctc gcgcgccttc
     1981 ttgaggtcgc ccacgagcgc agccgcgcac atctgatgca tcagctttgg cgccacattc
     2041 gcggtgaccg agatcacgcc gtggccgccg agcaggatca gcgccagcgc ggtcgcatcg
     2101 tcgccgctgt aaatggcgaa attgcgcggc gcgcgcttga tgaggtcggt gccccgctcg
     2161 atattcgccg tggcgtcctt gatgccgatg atccccggca cctgcgcgag ccgcagcatc
     2221 gtgtcgttgg cgagatcggc cacggtgcgc cccggcacgt tgtacacaat catgggcaga
     2281 tccaccgtct cggcgatctt gcggaagtga cggtacagcc cttcctgcgt cggcttgttg
     2341 tagtagggca ccaccgagag gcaggcggtc gcgccgttct tcttagcgga ctcggtcagc
     2401 tcgatcgctt ccgcggtgga gttgccgccg gtgccggcga tcacggggat gcgcccgcgc
     2461 gaatgctgca ccgctaggcg gatcagctct ttatgctcgt cgaaatccac ggtcggcgac
     2521 tcgccggtgg tgccgacgac cacgatgccg tcggtcccct ccgcggcatg ccagtcgatc
     2581 agttgcctga agcggccaag gtccaggcgg ccgtcctcgt gcatcggcgt gacgattgcg
     2641 accaggcttc cggtaatcat gcggggattc taacgcgact cgcgtcgcca cggaccacac
     2701 agatcctctg gcgcactgcg tttgcgctct cgccttgctc gaaagcaacc accgtcagga
     2761 cgcggaaagc ctccagaagc tcgcccggat gcaggaggaa ggcgggattc gacggcttgc
     2821 cgaaacgctc gttaccgagc atgaacgtct cgtagatgag cgccccgccc tcctcgagcg
     2881 ccgcggcgag gcggccgaaa atcggccggt gcagatagtt cgtcaccacg atcccgccga
     2941 atcgccggcc ggcgaacggc cacgggctgc catcttccag gtcggctttc acgaaccgta
     3001 cccccgggat cacccgcacc tcgcggtcaa ccgcaaccac ttcaagcccg cgcgccgcga
     3061 acagccgggc gtgccgcccg gctccgctcg cgacgtcgag caccgggccg cgcttgacca
     3121 gcggcgccca gcgcaccacc cacggcgaag gggttcgcag gtcgtgcggg tcagcggcca
     3181 atcttcgcgc c
//
LOCUS       DASUNT010000302         4446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_391910, whole genome shotgun sequence.
ACCESSION   DASUNT010000302 DASUNT010000000
VERSION     DASUNT010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391910"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFX94_06070"
     CDS             <1..437
                     /locus_tag="VFX94_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5767783.1"
                     /translation="LEELIDEVLLASRLDAVPALENVEEVDLLALAAEEAARYDAEAQ
                     GEPTTMRGDPRLLRRMIRNLLENARRHGAPPIAVSVRKRELRVCDAGPGVPPAERERI
                     FEPFNSKRGTGLGLALVRQIARRHGGEARCQGSCFCVTLPAG"
     gene            complement(500..1306)
                     /locus_tag="VFX94_06075"
     CDS             complement(500..1306)
                     /locus_tag="VFX94_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5767784.1"
                     /translation="MPLYRGMDRAALDAAYNNGAAVKESARIVAEWEERSARLRAAHP
                     EGMDLRYGPDERNRIDYFAARRDGPVLAFIHGGYWQMRSKEIFSFIAEGPLAWGINVA
                     LLGYTLAPQKRLDGIVAEIHAALSWLHSSIEVLGGDTNRLFVSGWSAGGHLTAMAMGH
                     AAVRGGLAISGIYDLEPMRLCYINDKLRLDTDEARRNSPGPTDKPLFIAYGGDELPEL
                     CRQSEDYAKVLGRKATAVPGKDHFTILEELASPHGALTALVRGLVSEKGS"
     gene            complement(1306..2292)
                     /locus_tag="VFX94_06080"
     CDS             complement(1306..2292)
                     /locus_tag="VFX94_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5767785.1"
                     /translation="MIRLTLACWDYDRTRALMDGTVRPEGIELVYLNQPVEETFFRMI
                     RYREFDCSEMSLSSYVASLSSEDPAFIAIPAFPSRFFRHSCIFVSTESGIRQPADLKG
                     KRIGVPEYQMTAPVWIRGILSDDYGVKVTDVEHYSGGEEESGRVEKLKLNLPPQIRLK
                     PIAVDKTLSRMLADGELDALITARAPSTFHTEPEKVKRLFPNYVEVEKEYYRRTKIFP
                     PMHTVVIRRDVYARHPWVAQSLYKAFAEAKSRAYALYHQTAALPAMVPWLVAEIEETR
                     REMGEDWWPYGLAPNRRALETFLRYHHEQGLSKRRFEPEELFARETLASFKI"
     gene            complement(2289..3425)
                     /locus_tag="VFX94_06085"
     CDS             complement(2289..3425)
                     /locus_tag="VFX94_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5767786.1"
                     /translation="MKTLRIGVAGLGRAFAVMAPAFRDPRVELVAAADPREEALLRFC
                     GEFKGRAYATVEELCRDPAVDVVYIATPHQFHAEHAAMACAHGKHVLVEKPMALSPNE
                     CRAMIQAAREARVTLVVGHSHSFDAPIVHAGKLIRGGAYGALRMITALDFTDFLYRPR
                     RPEELMTEAGGGAVFNQAAHQVDIARLLADARVRSVRAFTGIWDPARSTEGAYSCLLG
                     FENGVFASLVYSGYAHFDSDEFMGWIGELGQKKDPARHAMARRALAGDELALKSARNY
                     GGPQFEEAIPPAAHQHFGVFILSCEKADLRPLSNGVMIYADEERRFDPLQPPILPRGE
                     VIDELYAAVVEGSPPRHDGEWGMATLQVCFAILESARSGKEILL"
     gene            3440..>4446
                     /locus_tag="VFX94_06090"
     CDS             3440..>4446
                     /locus_tag="VFX94_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5767787.1"
                     /translation="MLTKEQNELLCRVEGAAPMGGVMRRHWLPVCMSEEVAEPDGKPV
                     RSRLLGEDLVVFRNTLNQIGVLGEYCLHRRASLAFGRNEDCGLRCLYHGWKFDVEGNV
                     VDMPSEAPGTAQRLRKRAKAYPVREGGGFVWAWMGPSQEMREFEPPAWAPRPDIRYAI
                     VKMHAACNWAQVLEGSIDSAHSSTLHSTNMPAAADVYGSTATDSAWLRPSNDKAPRLQ
                     FEATDYGFRYAAIRKPIRNPEADQYVRTTLFIAPFTVLIPPNNQYNLAQMLVPIDDVN
                     TMFYWIAWHADPAKGITQQAWRRFCAAEVGVDLDEHFRKKRTLENRYLQDREAMKRGD
                     WT"
BASE COUNT          753 a         1447 c         1495 g          751 t
ORIGIN      
        1 agctggagga gctgatcgac gaggtgctgc tcgcaagccg gctggacgcc gtgccggcct
       61 tggagaacgt cgaggaggtg gacctcctcg cgcttgccgc ggaggaggcg gcgcgctacg
      121 acgccgaggc gcagggcgag ccgaccacga tgcgcggcga cccgcggctc ctgcgccgca
      181 tgatccgcaa cctcctcgag aacgcgcgcc gccacggcgc gccgccgatc gcggtgtcgg
      241 tcaggaagcg cgagcttcgc gtctgcgacg ccgggccggg cgttcccccg gccgagcgcg
      301 agcgcatctt cgagccgttc aactccaagc gcggcacggg gctcggcctc gcgctcgtgc
      361 gccagatcgc gcgccgccac ggcggcgagg cgcgctgcca gggcagctgc ttttgcgtaa
      421 ctttacctgc cggataaaca gcgcttaccg tcagccccga gactctcctt cgtgccgaag
      481 cccggttccg atctcgaaat caggatcctt tttcgctcac gagcccgcgc acgagcgcgg
      541 tgagcgcgcc gtgcggcgac gcgagctctt ccagtatcgt gaagtggtcc ttgcccggaa
      601 cggccgtggc ctttcgtccc agaactttcg cgtagtcctc cgattgccgg caaagctcgg
      661 gcagctcgtc accgccatag gcgatgaaca gcggcttgtc ggtcggcccc ggactgttcc
      721 gccgcgcctc gtcggtgtcg agccgcagct tgtcgttgat gtagcaaagc cgcatcggct
      781 ccaggtcgta gattccactg atcgcgaggc cgccccggac cgcggcatgc cccatcgcca
      841 tcgcggtgag atgcccgccg gcggaccagc cggaaacaaa taagcggttc gtgtcgccgc
      901 cgagaacctc tatggaggaa tgcaaccagc taagggcggc atggatctcg gccacgatgc
      961 cgtcgaggcg tttctgcggc gcgagggtgt agcccagaag cgcgacgttg atgccccagg
     1021 cgagcggccc ttcggcaatg aaggagaaga tctctttcga gcgcatctgc caatagccgc
     1081 cgtggatgaa ggcgagcact ggaccgtcgc gccgcgcggc gaagtaatcg atgcgattgc
     1141 gctcgtcggg cccatagcgc agatccatgc cctcggggtg cgcggcgcgc agccgcgcgc
     1201 tgcgctcttc ccactcggca acgatccggg cgctttcctt caccgcggcg ccgttgttgt
     1261 aggcggcgtc gagcgctgcg cggtccattc ctcgatagag cggcatcaga ttttgaacga
     1321 cgccagcgtt tcgcgggcga aaagctcttc gggctcgaag cggcgcttcg acaggccctg
     1381 ctcgtggtgg tagcggagga aggtctcgag cgccctgcgg ttcggcgcga ggccgtaggg
     1441 ccaccagtcc tcgcccatct cgcgccgtgt ctcctcgatc tcggcgacga gccagggcac
     1501 catcgcgggc agggcggcgg tctggtggta gagcgcgtat gcgcggcttt tcgcctcggc
     1561 gaacgccttg tagagcgact gcgccaccca gggatgcctg gcgtagacgt cgcggcggat
     1621 caccaccgta tgcatcggcg ggaagatctt cgtgcgccgg tagtactcct tctcgacttc
     1681 gacgtaattc gggaagagcc gcttcacctt ttcgggctct gtgtggaacg tggagggcgc
     1741 ccgcgcggta atcagcgcgt cgagctcacc gtcggcgagc atgcgggaaa gggtcttgtc
     1801 aaccgcaatg ggcttcaaac ggatctgagg cggcaggttc agcttgagct tttccacgcg
     1861 cccggactcc tcctcgccgc cggagtaatg ctcgacgtcg gtgactttca cgccgtagtc
     1921 atccgacagg atgccgcgaa tccacaccgg cgcggtcatc tggtactcgg gcacgccgat
     1981 gcgcttgcct tttaggtctg caggttggcg gatgccactt tcggtcgaga cgaagatgca
     2041 tgagtgccgg aagaaccgcg aaggaaacgc cggaatggca atgaacgccg ggtcttcgga
     2101 gctaagagaa gccacatagg acgaaagcga catctcggag cagtcgaact cccggtagcg
     2161 gatcatgcgg aagaaggttt cttccaccgg ctggttgagg tacaccagct cgatgccttc
     2221 gggcctcacc gtgccgtcca tcagcgcacg cgtgcggtcg tagtcccagc aggcaagcgt
     2281 gaggcggatc aaagcaggat ttcctttccg ctgcgggcgg actcgagaat cgcaaagcat
     2341 acctgcagcg tcgccatccc ccactcgccg tcgtgccgcg gcggggagcc ctcgaccacg
     2401 gccgcgtaga gctcgtcgat gacttccccg cgcggcagta tgggtggctg cagcgggtcg
     2461 aagcgtcgct cctcgtcggc gtagatcatc acgccgttcg agagcggacg caggtcggcc
     2521 ttctcgcacg agaggatgaa cacgccgaaa tgctggtgcg cggccggggg aatggcttcc
     2581 tcgaactgcg ggccgccgta gtttcgggcg ctcttgagag cgagttcatc gcccgcgagc
     2641 gcacgccgcg ccattgcgtg ccgggcggga tccttcttct gcccgagctc gccgatccag
     2701 cccatgaact cgtcggaatc gaaatgcgca tagccgctgt agaccaggct tgcgaaaacg
     2761 ccgttctcaa agccgagcag gcacgaatag gcgccttcgg tcgaccgcgc gggatcccag
     2821 atgccggtga acgcccgcac gctgcgcacc ctggcgtcgg cgaggagccg cgcgatgtcg
     2881 acctggtgcg ccgcctggtt gaacaccgcg ccgccgcccg cttcggtcat gagctcctcc
     2941 ggccgccgcg ggcggtagag gaagtcggtg aagtcgagcg ccgtgatcat gcgcagcgcg
     3001 ccataggcgc cgccccggat cagctttccc gcgtggacga tcggcgcgtc gaagctgtgg
     3061 ctgtgcccga ccaccagcgt cactctggcc tcgcgcgcgg cctggatcat ggcgcggcat
     3121 tcgttcgggc tgagcgccat cggcttttcc acgagcacgt gctttccgtg cgcgcaggcc
     3181 atggccgcgt gctcggcatg aaactggtgc ggcgtggcga tatagacgac gtccaccgcg
     3241 gggtcgcggc aaagctcttc tacggttgcg taagcccggc ctttgaactc cccgcaaaat
     3301 ctcaggagcg cctcctcgcg cgggtcggcg gccgccacaa gctcgacgcg cgggtcgcgg
     3361 aacgcgggcg ccatgaccgc aaacgcgcgg ccaagtcccg cgacgccgat tcgcagggtt
     3421 ttcatgctat gtatacggca tgttaaccaa ggagcagaac gagctgctct gccgggtcga
     3481 aggcgccgcg ccgatgggcg gcgtcatgcg gcgccactgg ttgccggtct gcatgtcgga
     3541 ggaggtggcc gagcccgacg gcaagccggt acgttcgcga ctgctcggag aggatctggt
     3601 ggttttcagg aacacgctca accagatcgg cgttctcggc gagtactgtc tgcatcgccg
     3661 cgcctcgctt gccttcgggc gaaacgagga ctgcgggctg cgttgcctgt accacggctg
     3721 gaagttcgac gtcgagggca atgtcgtgga catgccttcg gaagctcccg gcacggcgca
     3781 gcgcctgcgg aagagagcca aggcttatcc cgtgcgcgaa ggcggcggct tcgtgtgggc
     3841 gtggatggga ccgagccagg aaatgcgcga gttcgagccc cccgcctggg cgccgaggcc
     3901 ggacatccgc tacgcaatcg tgaaaatgca cgccgcgtgc aactgggccc aggtgctgga
     3961 gggctcgatc gactcggcgc acagttcgac gttgcattcc accaacatgc cggcggcggc
     4021 ggacgtgtat ggctcgacgg cgaccgactc tgcctggctg cggccctcga acgacaaggc
     4081 gccgcgcctg cagttcgaag cgaccgacta cggcttccgc tacgcggcga tccgcaagcc
     4141 gatcaggaat cccgaggccg atcagtacgt gcgcaccacg ctgttcatcg cgccttttac
     4201 ggtgctgatc ccgccaaaca atcagtacaa cctcgcgcag atgctggtgc cgatcgacga
     4261 cgtcaacacg atgttctact ggatcgcctg gcacgccgac ccggcgaagg gcatcaccca
     4321 gcaggcgtgg cggcgcttct gcgccgctga agtgggcgtc gacctggacg aacacttcag
     4381 aaagaagaga actctggaga atcgttactt gcaggaccgc gaagcgatga agcgcggcga
     4441 ctggac
//
LOCUS       DASUNT010000303         4525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_128978, whole genome shotgun sequence.
ACCESSION   DASUNT010000303 DASUNT010000000
VERSION     DASUNT010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128978"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(319..774)
                     /locus_tag="VFX94_06095"
     CDS             complement(319..774)
                     /locus_tag="VFX94_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5767788.1"
                     /translation="MSQPKRSRSAHLDPSEYRQLASFRHALRRFLRFSEAAAEQVGLT
                     AQHYQALLAVCASADGRVTINDLAQQLLIRHNSAVGLVDRLTAQALLTREPSPEDGRK
                     VYLRLSSKGDRVLQKLAEVHREELRRIGPQLEALLHEITGTRLTGPPAA"
     gene            883..1431
                     /locus_tag="VFX94_06100"
     CDS             883..1431
                     /locus_tag="VFX94_06100"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX5767789.1"
                     /translation="MRIELPQLPYAASALEPWISARTVNTHHGRHHRAYVDKVKALVG
                     DNAETLEAVILRTAGDATRQVLFNNAAQAWNHAFCWQCLRPKSGPPPPAYEKWAAALK
                     EAALGHFGSGWAWLVRHGTQLKVITTANADTPMVHGLEPLLVIDVWEHAYYLDYQERR
                     AAYVTGVVDNLLNWELAECLTT"
     gene            1416..2132
                     /locus_tag="VFX94_06105"
     CDS             1416..2132
                     /locus_tag="VFX94_06105"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5767790.1"
                     /translation="MPDDLKATDIMTAPAITVGPDTSVAQVAALLANHCISGVPVEEG
                     GRLVGLVSEADLLHRHEIGTDGSVREQSGWRRLLGHHSSPAEYAKSHGLRVRDVMTRD
                     VVSIGADTPIDEIVALLEERKVKRVPVMRGAEVIGIFSRADVVRAVARQAQAPAEPAL
                     SDEAIEERLTAELERHHWWRTPYSSVRVENGVVHYSGIIDSPDEQAWRAAARVAAENL
                     AGVRAVEDHRFTMYDIPSMV"
     gene            2162..2572
                     /locus_tag="VFX94_06110"
     CDS             2162..2572
                     /locus_tag="VFX94_06110"
                     /inference="COORDINATES: protein motif:HMM:NF040052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6306 domain-containing protein"
                     /protein_id="HEX5767791.1"
                     /translation="MSRSEFGALLNTLLEAERAGAKLLAAYVAELPPNSTAWTALHKI
                     QRDEAHNCAVLIHLLLNAEVEPSMAIGDFYERGLRLCKWSERLDLVNRGQAWVAKRIA
                     EAVPRISEPGARTALRAMHESHVANIDLCERVFG"
     gene            2583..3356
                     /locus_tag="VFX94_06115"
     CDS             2583..3356
                     /locus_tag="VFX94_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5767792.1"
                     /translation="MQLSSVAVTKKPSDIDLVGRILSGDMRAMETLMRLHNRMLFRTA
                     HAILRDEAEAEEAVQDAYLKAYRNLATFRGESKLSTWLVRIVANEALMRRRRNPKPAE
                     RADQEPASEAPGPEEEAARAEVRSLVEAGINALPEGYRTVFVLRGLEEFSVEETALAL
                     GIPDATVRSRYFRARGLLRVWLAGGLDARLQDAFAFAGVRCERIVRAVLAVLGAAVAA
                     TTVAACSEKPPRPDPEAERPDKPLLERTLKQGEFGRMSY"
     gene            3525..4148
                     /locus_tag="VFX94_06120"
     CDS             3525..4148
                     /locus_tag="VFX94_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX5767793.1"
                     /translation="MERRLKISSGERLLDRALDEGIALEQECGGKLACASCRVVVLQG
                     MHHLVAASEDEVDMLERAGASAPNARLACQVRGTGDIVIEIPDAELPPACAASSPVRV
                     TPRAARHLAAQLNKHAGAVAVRLAVERSGCSGFSYRIDPAGAIGDGDAVFESHGVRIA
                     VDAASLPYVQGTLLDLVREGLAHRLQIDNPNVRESCGCGKSFSARSS"
     gene            complement(4189..4455)
                     /locus_tag="VFX94_06125"
                     /pseudo
     CDS             complement(4189..4455)
                     /locus_tag="VFX94_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003270151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
BASE COUNT          797 a         1465 c         1516 g          747 t
ORIGIN      
        1 atgccgagat atcacgaaca gtttggacgt cggctcaatg cgaacccggg aacccccggc
       61 gtcgttgact cacccactcg cggcagcagc accaaggatc attgggttgc attcctcggc
      121 ggcatcacgc ttatcgtcgt atcggcgctc gcgggcctgt atcgcggatc gttggaatgg
      181 acggacgtgc tgaccgccat cgttgggttc gccttactgg gcatggcagc ctacgatgcg
      241 cggccatcga gtgcaccttc ccggcaagag aaccgatcgc ggtgcacacc gccttccgcg
      301 ctgcctgcga acgggaagct acgccgccgg gggacctgtc agcctcgtgc cggtgatctc
      361 atgcaggagg gcctcgagct gcgggccgat gcggcgcagc tcctcgcggt gcacctcggc
      421 gagcttctgc aggacgcggt cgcccttcga gctaagcctg agataaacct tgcgcccgtc
      481 ctccggggag ggctcgcggg taagcagggc ttgcgcggtg agccgatcga cgaggccgac
      541 cgcgctgttg tgtcggatga ggagctgctg cgcaaggtcg ttgatggtga cccgcccatc
      601 ggcgctcgcg cacaccgcga gcagcgcctg gtaatgctgc gcggtgaggc cgacctgctc
      661 ggccgccgcc tcgctgaaac gcaggaaacg gcgcagcgca tgccggaacg acgcgagctg
      721 ccgatattca ctcggatcga gatgcgcgga gcggctgcgc ttcggttggc tcatggtcca
      781 ggcgggcgcc ccgcttatag gtgatctcaa catggttgac aatatatcgt aatacgacgt
      841 atatatatcg caccacgata tttaccgtca atggagcatc cgatgcgcat tgaacttccc
      901 cagctgccct atgcagcaag cgcgctcgag ccgtggatct cggcgcggac ggtcaacacg
      961 caccacggca ggcaccaccg cgcttacgtg gacaaggtga aagctctggt gggcgacaat
     1021 gccgagacgc tcgaagccgt catcctccgt accgcggggg atgccacgcg gcaagtgctc
     1081 ttcaacaatg ccgcccaggc gtggaaccac gcgttttgtt ggcagtgcct gcgaccgaaa
     1141 agcggaccgc cgccgccggc gtacgaaaag tgggcggcag cgctcaagga ggcggcgctc
     1201 ggtcactttg gcagcggctg ggcctggctc gtgcgccatg gtacgcagct caaggtgatc
     1261 accacggcaa acgccgacac gccgatggtg cacggcctgg agccgctgct cgtgatcgat
     1321 gtttgggaac acgcctacta cctcgactac caggagcggc gcgccgccta cgttaccggc
     1381 gtagtcgaca accttctcaa ctgggagctc gccgaatgcc tgacgacctg aaagccacag
     1441 acatcatgac cgcgcccgcg attactgtcg ggcccgacac atccgtcgcg caggtggcgg
     1501 cgctccttgc caatcactgt atcagcgggg ttcctgtcga ggagggcggc cgtctcgtcg
     1561 gcctggtcag cgaggccgac ctgctgcatc gccacgagat cggcaccgat ggcagcgtgc
     1621 gcgagcaatc cggctggcga cggcttcttg gtcaccattc gtcaccggcc gaatacgcca
     1681 agtcgcatgg ccttcgggtg cgcgacgtga tgacgcggga cgttgtctcg attggcgccg
     1741 acacgccgat cgacgaaatc gtcgcgcttc tggaagagcg caaggtcaag cgcgtccccg
     1801 tgatgcgcgg cgccgaagtc attggcatct tcagtcgcgc cgatgtggtg cgcgcagtgg
     1861 cgaggcaggc ccaggcgcct gcggagcccg cgctgagcga cgaagcgatc gaagagcggc
     1921 tcaccgccga gctcgagcgc catcactggt ggcgcacgcc ctattcgagc gtccgcgtcg
     1981 agaacggcgt cgtccattac tcgggaatca tcgattcgcc cgacgagcag gcgtggcgcg
     2041 ctgcggcgcg ggtggccgcg gaaaacctcg ccggcgtgcg cgcggtggag gatcaccgct
     2101 tcacgatgta cgacatccct tcgatggtat gaacacctta gcagcggaac gcacaatggc
     2161 catgtcgcga agcgagttcg gagcgcttct caatacgctc ctggaagccg agcgggccgg
     2221 ggcgaagctc cttgccgcgt acgtggccga gctgccgccg aattccaccg catggaccgc
     2281 gctgcacaag atccagcgcg acgaagcgca caactgcgcg gtgctcattc atctacttct
     2341 caacgccgag gtcgagccgt ccatggccat cggcgacttc tacgagcgcg gcctgcggct
     2401 ttgcaagtgg agcgagcggc tcgacttggt gaaccgcgga caggcgtggg tcgccaagcg
     2461 catcgccgag gccgtgccgc gtatttcgga gccgggtgca aggacggcgc tgcgcgccat
     2521 gcatgagtcg catgtggcga atatcgatct ttgcgagcgc gtgttcggtt aatggaggga
     2581 aaatgcaact cagtagcgta gcggtcacga aaaaaccgtc ggacatcgac ctcgttgggc
     2641 gcatcctttc cggcgacatg cgtgcgatgg agacgctcat gcgcctgcac aaccgcatgc
     2701 tgttccggac cgcgcacgcc atcctgcgcg acgaggcgga ggccgaggaa gccgtccagg
     2761 acgcgtacct caaggcgtac cgcaacctcg ccacctttcg cggcgagtcc aagctttcca
     2821 cctggctcgt gcgcatcgtc gcgaacgagg ccttgatgcg ccggcgccgc aatccgaagc
     2881 cggccgagcg cgccgaccag gagcccgcct ccgaggcgcc cggtcctgag gaggaagccg
     2941 cgcgcgcgga ggtgcggagc ctggtggagg caggcatcaa tgcgctgccc gagggctatc
     3001 ggacggtgtt cgtgctgcgc ggactcgagg agttcagcgt cgaggaaacc gcgctcgcgc
     3061 tcggcatccc agacgcgacg gtgcgctcgc gctatttccg ggcgcgtggc ttgctccgcg
     3121 tgtggctggc cggcggcctg gatgcgagat tgcaggacgc gtttgcattc gccggcgtgc
     3181 gttgcgagcg catcgtgaga gcggtactag cggtcttggg cgcggccgtc gcggcgacaa
     3241 ccgtcgcggc gtgcagcgaa aagccgccac ggcctgaccc tgaagccgaa cgaccggaca
     3301 agccgttgct cgagcgcacg ttgaagcagg gggaatttgg acggatgagc tattaagtca
     3361 gccgcctgga cgatcggtca ttccaatcgg ccgctgacgc agttcgggcg tgaaggacac
     3421 ggcgcagatg acttcacctg gtctggacta gtgatcgaca tgcttcactg agagctcggg
     3481 ctgcgcctgg ctgaacgccg agcctgcatg ccggtcctac cagtatggaa cggcggctga
     3541 agatctcgag cggggagcgc ctgcttgacc gcgccctcga cgagggaatc gcgctcgagc
     3601 aagagtgcgg cggcaagctc gcttgcgcat cttgccgggt ggtggtcctg caaggaatgc
     3661 atcatctcgt cgcggcaagc gaggacgagg tggacatgct cgaacgagcg ggagcgtccg
     3721 cgccgaacgc gcgtctcgct tgccaggtgc gcggcacggg cgacatagtc atcgagattc
     3781 cggacgctga gttgccgccc gcgtgcgccg caagctcacc cgtacgcgtc acgccgcggg
     3841 cggcgaggca tctcgccgcg cagctcaaca agcacgccgg cgcagtagcg gtgcggctcg
     3901 cggtcgagcg ctcgggctgc tcgggcttca gctaccgcat cgacccggca ggcgcaatcg
     3961 gcgacggcga cgccgtcttc gagagccacg gcgtgcgcat cgcggtcgac gccgcgagcc
     4021 tgccgtacgt gcagggcacc ctgctcgatc tggtgcgcga aggcctcgcg catcgcctgc
     4081 agatcgacaa ccccaacgtc cgtgagagct gcggttgcgg caagagcttc agcgcccggt
     4141 cttcctgatt tcgcgcgtgc gcctggtggc gcatgcgcca ggcggtgcaa gcagcccgag
     4201 catgcttcgc caaccaacgg ggagaacttc gctgcggcgc aagctgcgcc agatcgcttc
     4261 gaaccttgcg attccctggc ggtttggctc gcccgcgtag taggcgccgt tgattgctcc
     4321 gaccgacgaa cccacgacga gatcaggtac aaagccatgc gccacgagcg cgcgtagcat
     4381 gcccacctgc accgcgccga agctgccacc gcccgcgagc accaatgcgg tgctagtagc
     4441 cgtagaactc ttcattccgg atgtcctcgt cgctgacgcc agcgccgcgc aaggtctcct
     4501 gcatcttaat gaccatcgca ggtgg
//
LOCUS       DASUNT010000304         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1092599, whole genome shotgun sequence.
ACCESSION   DASUNT010000304 DASUNT010000000
VERSION     DASUNT010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092599"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="VFX94_06130"
     CDS             <1..1183
                     /locus_tag="VFX94_06130"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767794.1"
                     /translation="PAQAAGIAVLPFANHSADASREFFSDGLTEDVINALGRFSGIRV
                     IAHNAVQAYKHRTVAADEISRELAVRYVVQGSVREAGGRVRVGVELSDAMKGTLLWSE
                     RYDGEGREVFEIQDRIVRDIAGSLAVKLSALEQQRTAAKPPETLEAYELVLRARGLMR
                     AVQRAANREARALLAQAIRLSPNYAEAHAALAFAELQRAFFGWMEDPSDGLRRAEEAA
                     RRALAIDDAGANARAHAILGNIHTFTGNYEAALAEADRAIELNPSDAIARSLRGGILL
                     WTGKIEESIAASEAARRYDPRLPAEGMFNLALAYYLADRYREAVQSALAVQPSARTVF
                     LEAVHAGALAQLGEEDAARRVADEVRRLDPFFDVKRFGQRLVNPAHREKAQEGLRKAG
                     L"
     gene            complement(1191..1595)
                     /locus_tag="VFX94_06135"
     CDS             complement(1191..1595)
                     /locus_tag="VFX94_06135"
                     /inference="COORDINATES: protein motif:HMM:NF041384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HEX5767795.1"
                     /translation="MKKLMPALAALSLSGALLAQSPSISLRGYDPVAYFTEGRPVEGS
                     ADINYDFDDARYLFSSQKNRERFAATPDRYTPQYSGLCATGMALGAKAVADPTVWKIV
                     DGKLYVFSSPHAREKFESDPAMLAKSQQNWRK"
     gene            1638..>2123
                     /locus_tag="VFX94_06140"
     CDS             1638..>2123
                     /locus_tag="VFX94_06140"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5767796.1"
                     /translation="MRIATEEAWAPAELLARYRKLLQEKPASWDPGFHSLWGFFLGKT
                     PRATQLVERIQDLGARRLADMDASGIAKQVVFVTAPGVQVFDAATAVSLAGDLNDGLI
                     EQVGKYPQRYAALAAIAPQDPARSINELQRAVALGFKGAVINSHTQGEYLDEPKYWEI
                     FE"
BASE COUNT          353 a          725 c          728 g          317 t
ORIGIN      
        1 gccggcgcag gcggcgggca tcgccgtgct gcctttcgcc aaccacagcg cggatgccag
       61 ccgcgagttc ttcagcgacg gcctcaccga ggacgtgatc aacgcgcttg ggcgcttttc
      121 cggcatccgc gtcatcgcgc acaacgcggt gcaggcgtac aagcaccgca ccgtcgccgc
      181 ggacgagatc agccgcgagc tcgccgtgcg gtacgtggta cagggaagcg tgcgcgaggc
      241 cggcgggcgg gtgcgggtcg gggtggagct ctccgacgcg atgaagggta cgctgctatg
      301 gtcggagcgc tacgatggcg agggccggga ggtattcgag atccaggacc gcatcgtgcg
      361 cgacatcgcc ggctcgctcg cggtcaagct gagcgcgctc gagcagcagc gcacggcggc
      421 aaagccgccc gaaaccctgg aggcgtacga gctcgtcctg cgcgcccgcg ggctgatgcg
      481 cgcggtgcag cgcgcggcga accgcgaagc gcgcgccctg ctcgctcagg cgataaggct
      541 gtcaccgaac tatgccgaag cgcacgccgc cctggcgttc gccgagctgc agcgcgcctt
      601 tttcggctgg atggaagatc cctccgacgg gctgcgccgc gccgaggagg cggcgcggcg
      661 cgcgctcgcg atcgacgacg caggggcgaa cgcccgagcc cacgccatcc tcggcaatat
      721 ccacaccttc accggcaact acgaagcggc actggccgag gccgaccgcg ccatcgagct
      781 caacccgagc gacgccatcg cgcgctcgtt gcgcggcggg atcctgctct ggaccggcaa
      841 gatcgaagaa tcgatcgcgg cgagcgaggc cgcgcgccgc tacgaccccc gcctcccggc
      901 cgaaggcatg ttcaatctgg cgctcgccta ttacctggcc gaccgctacc gggaggccgt
      961 gcagagcgcg ctcgccgtcc agccgagcgc ccgcaccgtg ttcctcgagg ccgtgcacgc
     1021 cggggcgcta gcgcagctcg gcgaggagga cgccgcgagg cgcgtggccg acgaggtgcg
     1081 gcgcctggac ccgttcttcg acgtgaagcg tttcgggcag cgcctcgtga acccggcgca
     1141 ccgggagaaa gcgcaggaag gcctgcgcaa ggcaggcctc tgaccgactt tcacttccgc
     1201 caattctgtt gcgacttggc gagcatcgca gggtcgctct cgaacttctc gcgcgcgtgc
     1261 gggctggaga atacgtagag cttgccgtcg acgatcttcc ataccgtcgg gtcggccacc
     1321 gccttcgcgc caagcgccat cccggtcgca cacagcccgc tgtactgcgg cgtgtagcgg
     1381 tctggagtgg ccgcgaagcg ctcgcggttt ttctggctcg agaagaggta gcgcgcgtcg
     1441 tcgaagtcgt aattgatgtc agccgatccc tccaccggcc ggccttcagt gaaatacgcc
     1501 accgggtcgt agcctctcag gctgatcgag ggcgactggg cgagaagcgc gccgctcagg
     1561 gatagcgcag cgagcgcggg catcagcttc ttcatcctgt cctccttgca gcgacctggg
     1621 tcagctatct tacgcccatg agaatcgcga cggaggaagc ctgggcgccg gcggagttgc
     1681 tcgcgcgcta ccgcaagctg ctccaagaga agccggcgtc atgggacccg ggcttccaca
     1741 gcctgtgggg attctttctc ggcaagaccc cgcgcgctac gcagctcgtc gagcgcatcc
     1801 aggacctcgg ggcgcgccgc ctcgccgaca tggacgcgag tggcatcgcg aagcaggtcg
     1861 tattcgtcac cgcccccgga gtacaggtct tcgacgccgc gacggccgtg tcgctcgcag
     1921 gagacctgaa cgatggcttg attgaacaag tcggaaaata cccacagcgc tacgcggcgt
     1981 tagccgcgat cgcgccgcag gaccccgccc gctctatcaa cgagctgcag cgcgcggtcg
     2041 cgctcggctt caagggggcg gtgatcaact cgcataccca gggtgagtac ctcgacgagc
     2101 cgaagtactg ggagatcttc gag
//
LOCUS       DASUNT010000305         3145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_479605, whole genome shotgun sequence.
ACCESSION   DASUNT010000305 DASUNT010000000
VERSION     DASUNT010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479605"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VFX94_06145"
     CDS             complement(<1..581)
                     /locus_tag="VFX94_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX5767797.1"
                     /translation="MARRTSAAHDLSRIDTVGSAFRYFATVVIVLVAGMLVLNELGIS
                     MAPILATAGVAGIAIGFGAQSLIKDYFTGFFLLLEDQIRVGDVVEIVGKAGAVEELTL
                     RYVRLRDGDGYVHFIPNGEIKTVSNRTRIYAFANVDIGVPDEADFDAVFKAMRDVGAA
                     LRADTNFGGLVLDDTEVLGVERWELWGMLLRCR"
     gene            complement(717..1544)
                     /locus_tag="VFX94_06150"
     CDS             complement(717..1544)
                     /locus_tag="VFX94_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX5767798.1"
                     /translation="MGAPEPAAPMPQPPATFSYEHEGDRQFATTLARGLEVLRCFTPL
                     EPLLGNKEISVRTGLPKPTVSRLTYTLTKLGYLRHNMRLGKYQLGSAVLSIGYPLLAS
                     MNVRQVARPLMKELADYCNGSVSMGVRDRLSMVYVESCRSGNGITTLPDIGTSVPISQ
                     SVIGRAFLSACTPLERESVLNQMKVKEPEAHRKFRPSIDKALEDIRTRGFCASTGELR
                     REVYAVGAPMRRTVDGEIVSFNCIVPGFMVKKGQLEDDLGPRLVAMVRNIEASLGLH"
     gene            1687..2442
                     /locus_tag="VFX94_06155"
     CDS             1687..2442
                     /locus_tag="VFX94_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5767799.1"
                     /translation="MFELQGKVALVTGATRGIGRSIAEELARAGARVALCSRKEEACQ
                     QVREELESKGFQVHAQACNVSRRQELQGLVDAAAAKWGGIDIVVANAASNPYYGPLTG
                     IDDEAFDKIFANNLKSVLWLAAMTLPGMAARGGGSFILVGSIGGILANTVIGAYGMSK
                     AAGHHLVRNLAAEWGPQNVRVNAIAPGLIRTEFARALWEDEKRRAERIAATPLRRLGE
                     PRDIGGIAVFLASPAAAFITGQCIVADGGVTIL"
     gene            2445..>3145
                     /locus_tag="VFX94_06160"
     CDS             2445..>3145
                     /locus_tag="VFX94_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5767800.1"
                     /translation="MDLNYSPEELAFRDEVRAWLAANLPQELKDRSHLSKEELMRWHR
                     ILARQGWVAPAWPREWGGTGWNVVQRYIFEEELGYAGAPPIIPFGINMCAPVLLAFGT
                     EAQKKRFLPRIYNGDDFWCQGYSEPGSGSDLASLKTKAARAGEGYVVNGQKIWTSRAL
                     YSDLMLLLARTTPLDQVKKRTEGLSVFLVDMREAVGNGMTIRPIKAMINHNTTEVFFD
                     NLRIPASSLIGEEGRG"
BASE COUNT          559 a         1017 c         1058 g          511 t
ORIGIN      
        1 aagcggcagc gcagcagcat gccccacagc tcccagcgct cgaccccgag cacctcggta
       61 tcgtcgagga caaggccgcc gaagttggta tcggcacgca gcgccgcgcc gacatcgcgc
      121 atggccttga agaccgcgtc gaagtccgct tcgtcgggca cgccgatgtc gacgttcgcg
      181 aaagcgtaga tgcgcgtgcg gttggaaacg gtcttgatct ccccgttcgg gatgaagtgg
      241 acgtagccgt cgccgtcgcg caggcgcacg tagcgcagcg tgagttcctc gaccgcgccc
      301 gccttgccga cgatctcgac gacatcaccg acgcggatct gatcctccag cagcaggaag
      361 aagcccgtga agtaatcctt gatgaggctt tgcgcgccga acccgatcgc gatcccggcg
      421 acgcccgccg tggcgagtat cggcgccatc gagatgccaa gctcgttcag caccagcatc
      481 ccggccacca gcacgatcac cacggtcgcg aagtagcgga acgccgagcc gacggtatcg
      541 atgcggctga gatcgtgggc ggcgctcgtg cgccgcgcca tgtagccgcg gaaggcgcgg
      601 atcaggcgcg aggcgatcgc ctggatggcc caggcgatcg ccaggatcag cgcaatgcgc
      661 gcgaggctgc gcgcctcgcc gctcagctcg cggtagacgg tgagaacgtc ttccagttag
      721 tggagaccca gcgaggcctc gatgttccgc accatcgcga ccagccgcgg cccgaggtca
      781 tcctcgagct ggcccttctt caccatgaat ccgggcacga tgcagttgaa ggagacgatc
      841 tccccgtcca ccgtgcggcg catgggggcg ccgaccgcgt agacctcgcg ccgcagctcg
      901 cccgtgctcg cgcagaagcc gcgcgtacgg atgtcctcga gagccttgtc gatcgagggc
      961 cggaacttgc ggtgcgcttc cggctccttg actttcatct ggttcagcac cgattcgcgc
     1021 tcgagcggcg tgcaggccga gaggaacgcg cgcccgatga ccgactgaga gatcggcacc
     1081 gaggtgccga tgtcgggcag cgtcgtgatg ccattgccgc tgcggcagga ttcgacgtac
     1141 accatgctca ggcggtcgcg cacgcccatc gagaccgagc cgttgcagta gtcggcgagc
     1201 tccttcatca gcgggcgcgc cacctggcgc acattcatcg atgcgaggag cgggtagccg
     1261 atcgaaagca ctgcggatcc gagctggtat tttcccaggc gcatgttgtg gcgcaggtag
     1321 ccgagcttgg tcagcgtata ggtcaggcgc gacaccgtgg gcttgggcag cccggtgcgc
     1381 accgagatct ccttgttgcc gagcagcggc tcgagcggcg tgaagcagcg caggacttcc
     1441 agcccgcgtg cgagcgtggt ggcgaactgc cggtcgccct cgtgctcgta gctgaacgtg
     1501 gccggtggct gcggcatcgg cgccgcgggt tcgggtgctc ccatgaaaaa aagcctcctc
     1561 gttggctttc gtgttgcggg tttggaaaaa gcagggcgca gtgtttcgcg tttcgttatt
     1621 ctgtcggccc ggcgccgcgt gtcaacttag tcatacggaa tttcgcattg cggaatcagg
     1681 cagcccatgt ttgaacttca agggaaagtg gccctcgtca ccggggcgac gcgcggcatc
     1741 ggaagatcga tcgcagagga gctcgctcgc gccggcgcgc gcgtcgcgct ttgcagcagg
     1801 aaggaagaag cgtgccagca ggtaagagaa gaacttgaat caaaagggtt ccaggtgcat
     1861 gcgcaagcgt gcaacgtatc gcgcaggcag gagctccagg ggctggtcga tgctgcggcc
     1921 gcaaaatggg gcggcatcga catcgttgtg gcgaacgcgg cttccaaccc gtactacggc
     1981 ccgttgacgg ggatcgacga cgaagccttc gacaagatct tcgccaacaa cctgaagagc
     2041 gtgctgtggc ttgcggcgat gacgcttccc ggaatggcgg cccggggcgg aggcagcttc
     2101 atcctggtcg gctcgatcgg cgggatcctc gccaacaccg tgatcggcgc ctacggcatg
     2161 tccaaggccg ccggccacca cctggtgcgc aacctcgccg ccgagtgggg gccgcagaac
     2221 gtgcgggtga atgccatcgc gcccggcctc atccggaccg agttcgcccg cgcgctctgg
     2281 gaggatgaga agcggcgcgc cgagcgcatc gccgccacgc cgctgcgccg cctgggagag
     2341 ccgcgcgaca tcggcggcat cgccgtcttc ctcgcgtcgc cggcggccgc gttcatcacc
     2401 gggcaatgca tcgtcgccga cggcggagtt actatcctgt agccatggat ctcaactact
     2461 cccccgaaga gctcgccttc cgcgacgaag tccgtgcctg gctggcggcc aacctgccgc
     2521 aggagctgaa ggaccgcagc cacctttcca aggaagagct gatgcgctgg catcgcatcc
     2581 tggcgagaca gggctgggtg gcgcccgcgt ggcccaggga gtggggcggc accgggtgga
     2641 atgtggtgca gcgctatatc ttcgaggaag agctcggcta cgccggagcg ccgccgatca
     2701 ttcccttcgg catcaacatg tgcgcgccgg tgctgctcgc tttcggcacc gaggcgcaga
     2761 aaaagcgctt cctgccgcgc atctacaacg gcgacgactt ctggtgccag ggctattccg
     2821 agcccggctc gggcagcgac ctcgcgtcgc tgaagaccaa agccgcgcga gccggcgagg
     2881 gctatgtcgt caacgggcag aagatctgga cttcgcgcgc gctctattcg gacctgatgc
     2941 tgctgctcgc gcgcaccacg ccgctcgacc aggtgaagaa gcggaccgag ggcctgtcgg
     3001 tattcctggt cgacatgcgc gaggcggtcg gcaacggcat gaccatccgc ccgatcaagg
     3061 cgatgatcaa ccacaacacc accgaagtgt tcttcgacaa ccttcgcatc ccggcgagca
     3121 gcctgatcgg cgaggaagga cgcgg
//
LOCUS       DASUNT010000306         5415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1180882, whole genome shotgun sequence.
ACCESSION   DASUNT010000306 DASUNT010000000
VERSION     DASUNT010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180882"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..740)
                     /locus_tag="VFX94_06165"
     CDS             complement(126..740)
                     /locus_tag="VFX94_06165"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5767801.1"
                     /translation="MLAAILFSIALPAQAALTAAASELIGKYVIDPARADLGRVHDLI
                     IDLREGRVHYAVLGFAGWAGIFDKQFAIPMEEFTPAPYSDKLVLERAKDLLLLEPGID
                     STGVPDGEAEYWRGIDRWYERHDTRGLPLRQYARASRLIGRSLVDRTGEPIGELRDLR
                     VDLASGRVLDVLLALEGDARSVPLSMLRVPSSGAAIVLNSGERP"
     gene            881..1852
                     /locus_tag="VFX94_06170"
     CDS             881..1852
                     /locus_tag="VFX94_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012406396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5767802.1"
                     /translation="MRTVVVTGASAGIGRAAARAFARRGWNVALIARGAAGLAAAAAE
                     VERAGGRALTMQCDVADFRAVEEAASRAERELGPIAVWVNNAMVTVFCDALSVTPEDF
                     RRATEVTYLGAVWGTRVALARMKPRNRGAIVQVGSALSYRSIPLQSAYCGAKAALRGF
                     TDALRCELLHDGSRVHLTMVHLAAFNTPQFEWARTCMPRRPRPLGTIFQPELAGEAIY
                     WAAQHHRRELWVGWPAVQAILGQRLVAPLLDRLLARRAYEGQFSEQPPPSGREDNLYQ
                     PVEEDRGAHGRFDHLAVSTSGQHWANTHRGLLAAALIGLGWLCLRNR"
     gene            1863..2207
                     /locus_tag="VFX94_06175"
     CDS             1863..2207
                     /locus_tag="VFX94_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767803.1"
                     /translation="MALARVALDHHAHDRRPLQGDHEVGDASAEKRLRAYEFAGTLWG
                     GELGAIGEHESVALQPRAQRHGEHRLVDDDSWAITGMVVDTRNWLPGRKVMVPPSAIS
                     GIDWERSTVNVR"
     gene            complement(2242..2700)
                     /locus_tag="VFX94_06180"
     CDS             complement(2242..2700)
                     /locus_tag="VFX94_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767804.1"
                     /translation="MRALRFVALALTALTLGLAFSHVLEMPAKLAYSGSQWLLLQQTL
                     YGNFRALGMLIELGAVASAIALVYVVRARRPALYWTFFAATCLVAAHVAWWTGAFPVN
                     AVVAQHTPQMLPPDLAQLRQQWEHTHVVRAVLQGAALGALLASLLAETRR"
     gene            complement(2705..3004)
                     /locus_tag="VFX94_06185"
     CDS             complement(2705..3004)
                     /locus_tag="VFX94_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5767805.1"
                     /translation="MRAAVVAVAAALSPPLALADELGKKVFTSVAQPSCALCHTLEAA
                     GAKGKLGPSLDELKPDEARALKAIKQGVGVMPSYADNLSPEEIAAVARYVAESVR"
     gene            complement(3001..4182)
                     /locus_tag="VFX94_06190"
     CDS             complement(3001..4182)
                     /locus_tag="VFX94_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEX5767806.1"
                     /translation="MNPRAIDFGRRRLMLAGGAAGLAGFAARGRAQGKPLPEYADFKD
                     PADVIVHSANTIETERTAFGTSGVTPEDILFVRNNLKPEPAFVANRDAWRIEVDGVAR
                     PRTFTLEELKRIGLGTLSMVLQCSGNGRGFFDHKASGTQWRVGAAGNTLWSGVPVKDL
                     VNMLGGVREGMRYMTSTGGEIIPPGIDPKSVIVERSVPWTEWEHALLAWELNGEPIPL
                     AHGGPLRIVIPGYYGINNVKYVKRLAFTLEESDAAIMRTGYRVRPIGMKGAPTQPTMW
                     EMNLKSFITHPAGDKPLAPGTVQLHGVAFSGGGGVQGVQVSVDGGRTWWKAPFFGPYL
                     GRHAWQQFLLPVRLERGTHVIASRALGADGAVQPEHRLENERGYGHNGWRDHAVRVSV
                     A"
     gene            4273..4692
                     /locus_tag="VFX94_06195"
     CDS             4273..4692
                     /locus_tag="VFX94_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767807.1"
                     /translation="MRTILIALLFAAPAQAQLLPGANDLFLPPHGDEGCWVSFFAGRN
                     FTPPAAQLAGRSYVESFATEIPAAVKPELDHVGGESFFADVSSLIVGPHARLVGYGER
                     NFRGRELVIGPGEYVSELADIHFHERVRSFKLFCEAT"
     gene            complement(4685..5038)
                     /locus_tag="VFX94_06200"
     CDS             complement(4685..5038)
                     /locus_tag="VFX94_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5767808.1"
                     /translation="MRRWSSAFLALLAAACGDDQAPALGGNPEQGKLLLRQFACTSCH
                     TIPGVAGGDGKVGPPLAGVAGRIYLAGVLPNTPQTMASFVRYPQKADPKTAMPDMGVT
                     EEQARDMVAYLYQLK"
     gene            5173..>5415
                     /locus_tag="VFX94_06205"
     CDS             5173..>5415
                     /locus_tag="VFX94_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEX5767809.1"
                     /translation="MEARTTRLSQPARYEFFSAEEARFVEAALARLIPVDELGPGALE
                     AGVQYFMDRQLGGTYGAGERLYQAGPWRKGTSTQGYQ"
BASE COUNT          827 a         1844 c         1878 g          866 t
ORIGIN      
        1 cgcttcgtgt gccggttgct cctcggtgat gcgcagcacc tcctgcggct ccgaggcgcc
       61 gcacgccgtg agggcgagca cgccaatcag aatcgcgcgg ctgaggaaaa cgctcgggaa
      121 tgcgctcatg gtctctctcc agagttcaat acgatggccg cgccgctcga aggcacgcgc
      181 agcatcgaca gcggcacgct gcgcgcatcg ccttcgaggg cgagcaggac atcgagcacg
      241 cggccgctcg ccagatcgac gcggaggtcg cgcagctcgc cgatcggctc gccggtgcgg
      301 tcgacgaggc tgcgcccaat gagccggctc gcgcgcgcgt actgccgaag cggcagtcct
      361 cgcgtgtcat gacgttcgta ccagcgatca atgccgcgcc agtactcggc ctcgccgtcc
      421 ggcaccccgg tgctgtcgat accgggctcg agaagaagaa ggtccttggc gcgctcgagc
      481 accagcttgt cgctgtaggg cgcaggcgtg aactcctcca tcgggatggc gaactgcttg
      541 tcgaaaatgc cggcccagcc ggcaaagccg agcaccgcgt agtgcacgcg gccctcacgc
      601 agatcgatga tcaggtcatg cacgcgcccg aggtcggcgc gcgcggggtc aatcacgtac
      661 ttgccgatga gctcgcttgc cgcggcggtg agcgcggctt gggccggcag cgcgatggaa
      721 aaaaggattg ctgcgagcat gcgcatactc gggccgccgc aaggcgcatg ccccggtttt
      781 cggatgcggc gagacgcgcg gcccgctgga gcgcgtgtaa gcgtttcagc ggaccgcgcg
      841 caaaccttgc gcaggcacgc tacctgcgcg gcgctcgctc atgagaaccg tcgtcgttac
      901 cggagcgtcc gccggcatcg gccgcgccgc ggcgcgcgcc ttcgcgcgtc gcggctggaa
      961 cgtcgcgctc attgcacgcg gtgccgccgg cctcgccgcg gcggcggcag aggtggagcg
     1021 cgccggcggg cgcgcgctga cgatgcagtg cgatgtggcc gatttccgtg cggtcgagga
     1081 ggcggcgagc cgcgccgagc gcgagctcgg gccaatcgcg gtctgggtga ataacgccat
     1141 ggtcaccgtg ttctgcgatg cgctgtccgt gacgccggaa gatttccgcc gcgcgaccga
     1201 agtcacctac ctgggcgccg tttggggcac gcgcgtggcg ctcgcacgca tgaagccgcg
     1261 caaccgtggt gcgatcgtgc aggtcggctc ggcgctctcc taccgttcga tcccgctgca
     1321 gtcggcgtac tgcggcgcga aggccgcgct gcgcggcttc accgacgcgc tgcgctgcga
     1381 gctgctgcac gacggcagcc gcgttcacct caccatggtg caccttgcgg ccttcaatac
     1441 gccgcagttc gaatgggcgc gaacctgcat gccgcggcgg ccgcgtccgc tcggcacgat
     1501 cttccagccc gagcttgcgg gcgaggcgat ctactgggcg gcgcagcacc accggcgcga
     1561 gctgtgggtg ggctggcccg cagtgcaggc catcctcggc cagcgcttag tcgcgccgct
     1621 ccttgaccgg ttgctcgcgc ggcgcgcgta cgagggccag ttctccgagc agccgccgcc
     1681 gtcgggccgc gaagacaatc tctatcagcc cgtcgaggag gaccgcggcg cgcatggacg
     1741 cttcgaccac ctcgcggttt cgacgagcgg ccagcactgg gcgaacacgc accgcggcct
     1801 gctcgccgcc gcgctcatcg gccttggctg gctgtgcctg cgaaatcgct gaatgccacg
     1861 gtgtggcgct cgcccgagtc gctctcgatc accacgctca cgaccgtcgc ccgctccagg
     1921 gcgatcatga ggtcggcgac gcgagcgccg agaagcgtct tcgcgcgtac gagttcgccg
     1981 gcactttgtg gggcggggaa ctcggcgcga tcggcgagca cgagtcggtc gcgctgcagc
     2041 cccgggcgca gcggcacggc gaacaccgcc tggtggacga cgatagctgg gcgatcaccg
     2101 gcatggtcgt ggacacccgc aactggctgc ccggaagaaa agtcatggtg ccgccgagcg
     2161 cgatctccgg catcgactgg gagcgctcca cggtcaacgt acgttgagcc gggaggagtt
     2221 gaagcgggcg cccgaagcgc gctaacgccg cgtctcggcc agcagtgagg cgaggagagc
     2281 gccgagcgcc gcgccctgga gcacggcgcg cacgacgtgc gtgtgctccc actgctgccg
     2341 cagctgcgcc agatcgggcg gcagcatctg cggtgtgtgc tgcgcgacaa ccgcgttcac
     2401 cggaaacgcg cccgtccacc acgccacgtg ggcggcgacg aggcaggtgg cggcaaagaa
     2461 ggtccagtag agcgccggcc ggcgcgcgcg cacgacgtag acgagcgcga tcgcgctcgc
     2521 caccgcgccg agctcgatca gcatgccgag cgcgcggaag ttgccgtaga gcgtctgctg
     2581 caggagcagc cactgcgaac ccgagtaggc gagcttcgcc ggcatctcga gcacatgcga
     2641 gaaagcgagg ccgagcgtga gcgctgtgag cgcaagcgcg acaaagcgca gcgcacgcac
     2701 cgcttcagcg cacgctctcg gcgacgtaac gcgccaccgc ggcgatctct tccggcgaga
     2761 ggttgtcggc gtacgacggc atcacgccca cgccctgctt gattgccttg agcgcgcgcg
     2821 cctcgtcggg cttaagctca tcgagcgacg gcccgagctt tcccttcgcg ccggccgcct
     2881 cgagcgtgtg gcaaagcgcg caggaaggtt gggcgacgct cgtgaagact ttcttgccga
     2941 gctcgtccgc taaagcgagc ggcggcgaaa gcgccgctgc aacggcgacg acagcagccc
     3001 tcacgccacg ctgactctta ccgcgtggtc gcgccagcca ttgtggccgt agccgcgctc
     3061 gttttccagg cgatgctcgg gctgcactgc gccgtcggcg ccgagagcgc ggctcgcgat
     3121 gacgtgcgtg ccgcgctcaa ggcgcaccgg cagcaggaac tgttgccagg catgccggcc
     3181 gagatagggg ccgaagaacg gcgctttcca ccatgtgcgg ccgccgtcga ccgacacttg
     3241 cacgccctgc acgccgccgc cgccggagaa tgcgacgccg tggagctgca ccgtgcccgg
     3301 ggcgagcggc ttgtcgccgg caggatgcgt aatgaacgac ttgagattca tctcccacat
     3361 cgtgggctgg gtcggcgcgc ctttcatgcc gattgggcgc acgcgatagc cggtgcgcat
     3421 gatggccgcg tcgctctcct cgagggtaaa agccaggcgc ttcacgtact tcacgttatt
     3481 gatgccgtag tagccgggga tcacgatgcg cagcggcccg ccgtgcgcga gcgggatcgg
     3541 ctcgccgttc agctcccagg cgagcaaggc gtgctcccac tcggtccacg gcaccgagcg
     3601 ctcgactatt acgctcttcg gatcgatgcc cggcgggatg atctcgccgc cggtgctcgt
     3661 catgtagcgc atgccttcgc gcacgccgcc tagcatgttc accaagtcct tcaccggcac
     3721 gccgctccag agcgtgttgc cggcagcgcc gacgcgccac tgcgtgccgc tcgccttgtg
     3781 gtcgaagaac ccccggccgt tgcccgagca ttgcagcacc atggagagcg tgccgagccc
     3841 gatgcgcttc agctcctcga gcgtgaacgt gcgtgggcgc gcaacaccgt cgacctcgat
     3901 gcgccaagca tcgcggttcg cgacgaatgc tggctcgggc ttgagattgt tgcgcacgaa
     3961 aaggatgtcc tccggcgtaa cgccgctcgt gccgaacgca gtgcgctcgg tttcgatcgt
     4021 gttcgcgctg tgcacgatca cgtcggcggg gtctttgaag tcggcgtatt cgggaagtgg
     4081 cttgccctgc gcgcgcccgc gcgcggcaaa gcctgcgagg ccggcggcgc cgccggcgag
     4141 catcaggcgg cggcggccga agtcaatggc tcgtggattc atggcgctcc tttcagaatg
     4201 cgcgcatggt cgcgcgcttc gcattaagtg cgccttaagg acgttgggtg cgaaccttgc
     4261 tcgccgacag gcatgcgcac gattctgatc gctcttctct tcgcggcgcc agcccaggcg
     4321 caactcctgc ccggcgccaa cgatctcttc cttcccccgc acggtgacga aggttgctgg
     4381 gtatcgttct tcgccggacg caatttcacg ccgccggcgg cgcagctcgc cgggcgcagc
     4441 tatgtcgagt cattcgcgac cgaaataccg gcggcggtga agcctgagct cgatcacgtc
     4501 ggcggcgagt cgttcttcgc cgatgtttcg agcctcatcg tcggaccgca cgcccggctc
     4561 gtcggctatg gcgagcgcaa cttccgcggc cgcgagctgg tcatcgggcc aggcgagtac
     4621 gtgagcgagc tcgccgacat ccatttccac gagcgtgtgc gctcgttcaa gcttttctgc
     4681 gaggctactt gagctggtag aggtaagcga ccatgtcgcg cgcttgctcc tcggttacac
     4741 ccatgtccgg catggccgtc ttcggatccg ccttctgcgg gtagcgcacg aaggacgcca
     4801 tggtctgcgg ggtgttgggc agcacgccgg cgaggtagat ccgcccggcc acgccggcga
     4861 gcggcggccc gaccttgccg tcgccgccgg cgacgccggg gatggtgtga cagctcgtgc
     4921 aggcgaactg gcggaggaga agcttcccct gttcggggtt gccgccgagc gcgggcgctt
     4981 gatcgtcgcc gcacgccgca gcgagcagcg cgagaaatgc cgaactccat cgccgcatag
     5041 cagtcggacc tctgtgatgc gcgcgcattc ccgcctggcg caaagtttct cgtgagcaag
     5101 cccccgagtc catcgcgtcg gcgtttcatt caggcgggaa ccggctttgc cgtcgccgtc
     5161 ccgctacgcc gcgtggaggc gcggacgacg cgcctttcgc agcccgcgcg ttatgaattt
     5221 ttttcagcgg aagaggcgcg cttcgtcgag gcggcgctcg cgcgtttgat accggtggac
     5281 gagctcggcc cgggcgcgct ggaggccggc gttcagtact tcatggaccg ccagctcggc
     5341 ggtacatacg gcgccgggga gcggctctac caggcgggcc cttggcgcaa aggcacttcg
     5401 acgcagggct accag
//
LOCUS       DASUNT010000307         2276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_480161, whole genome shotgun sequence.
ACCESSION   DASUNT010000307 DASUNT010000000
VERSION     DASUNT010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480161"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1887
                     /locus_tag="VFX94_06210"
     CDS             <1..1887
                     /locus_tag="VFX94_06210"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEX5767810.1"
                     /translation="QARAERSGWLRAHTVPEPDLAAFSNRWLHADAGVDSEPYVRAAD
                     RIAEGLLDIFALRDVELGAPPRWNRDPKTGIEAPLEFGKALDYRDADLVGDIKYLWEP
                     NRHAHLVTLAQAWALTGKAAYFNAIVEQLESWFLACPYGYGPNWSSSLELALRLLNWS
                     AAWQLLGREAIDEHPGFRDRWLKSAYRHAQFIRGWLSLHSSANNHLIGEAAGLFIAGL
                     AWPHWPAAREWARAGKEILEREALAQNAPDGVNREQAVWYQQFVLDLMVSALLAGKAN
                     GQWFSADYESRIEQMMDFIASLMDAGGNVPMIGDADDGLASPLSPEIRACPYKSLLAL
                     GAILFSRGDFKLKAGALDDRARWLLGAGAQARFADLDVEKTRLPLRQAFPEGGYFILG
                     CEFDTPNEIRLVADAGPLGYRSIAAHGHADALSFTLSVGGEEFLIDPGTYAYHTQERW
                     RQYFRGTAAHNTVRIDGLDQSVPGGSFMWLKHARAGCSLWLSSADQDSFEGWHDGYMR
                     LEDPVKHRRLIQVQKKTRRVLIEDTLEMEDEHDVELFLHCSEHCDVEPLPGGYLLSAA
                     GTSIKVVVPQSENAVHAVQRASVAPIAGWVSRAFDRREPASTSVWRARLSGLSRLRTV
                     ITIF"
     gene            1897..>2276
                     /locus_tag="VFX94_06215"
     CDS             1897..>2276
                     /locus_tag="VFX94_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767811.1"
                     /translation="MVRKSKTQQLTDSILGQHLALSVAAHLARTQLVPEPLAVYDSEH
                     LSEMIDVVARALVRVAPLYVRDAGSGEPRELTAFELEGALIARAATLLTLKDGRSFSS
                     ASIKRADLRQAIVILKAVGVDELAP"
BASE COUNT          349 a          790 c          767 g          370 t
ORIGIN      
        1 caggcgcgcg ccgagcgctc cgggtggctg cgcgcgcaca cggttcccga gccggacctc
       61 gcggcttttt cgaaccgctg gttacacgcg gacgccggcg tcgactccga accttacgtg
      121 cgcgcagccg accgcatcgc cgaaggcttg ctcgatatct ttgcgctccg cgacgtcgag
      181 ctcggcgcgc cgccgcgctg gaatcgcgat ccgaagaccg ggatcgaggc accgctcgag
      241 ttcggcaagg cgctcgacta ccgcgacgcc gacttggtcg gcgatatcaa gtacctgtgg
      301 gagcccaacc ggcacgcgca cctcgtgacg ctcgcgcagg cctgggcgct caccggcaag
      361 gcggcctact tcaacgccat cgtcgagcaa ctcgagagct ggttcctcgc ctgcccctac
      421 ggctacgggc ccaactggtc gagctcgctc gagctcgcgc tgcgccttct caactggtct
      481 gccgcctggc agcttctcgg ccgcgaagcg atcgatgagc atcccggctt ccgcgatcgg
      541 tggctgaaat ccgcgtatcg ccacgcgcag ttcatccgcg ggtggctctc gctccactcc
      601 tcggcgaaca accacctgat cggcgaggcg gcggggctgt tcatcgccgg gctcgcctgg
      661 ccgcactggc cggcggcgcg cgagtgggca agggccggga aggaaatcct cgagcgggag
      721 gcgctggcgc agaacgcgcc cgacggggtg aaccgcgagc aggccgtgtg gtaccagcag
      781 ttcgtcctcg acctcatggt gagcgcgctg ctcgccggca aggcgaacgg ccagtggttc
      841 tcggccgact acgaatcgcg catcgagcag atgatggact tcatcgcctc gctcatggac
      901 gccggcggca acgtcccgat gatcggggat gcagacgatg gcctcgcttc gccgctttcc
      961 cccgaaatca gggcctgccc gtacaaatcg ctcctcgcgc tgggggcgat cctcttcagt
     1021 cgcggcgact tcaagctcaa ggcgggggcg ctggacgacc gggcgcgctg gctcctcggc
     1081 gcgggcgcgc aggcccggtt cgcggatctg gacgtcgaaa agacgcggct gccgctgcgc
     1141 caggcgttcc ccgagggggg ctacttcatt ctcggctgcg agttcgacac gccgaacgag
     1201 atccgcctgg tcgccgacgc cggcccgctc ggctaccgct cgatcgcggc gcacggtcac
     1261 gccgacgcgc tttccttcac gctctcggtg ggcggcgagg aattcctcat cgaccccgga
     1321 acttacgcct atcacacgca ggagcgctgg cgccagtatt tccgcggcac ggcggcccac
     1381 aacaccgtgc gcatcgatgg gctggaccag tcggtgcccg gcggcagctt catgtggctg
     1441 aaacatgctc gcgccgggtg cagcttgtgg ctttcttccg cggaccagga cagcttcgag
     1501 ggctggcacg acggctacat gcgcctggag gacccggtga agcaccgccg gctcatccag
     1561 gtgcagaaga agacccgccg ggtgctgatc gaggacacgc tcgagatgga ggacgagcac
     1621 gacgtcgagc ttttcttgca ctgcagcgag cactgcgacg tcgagccgct gcccggcggc
     1681 tatcttctga gcgccgccgg cacatcgatc aaagtcgtgg tcccgcagtc cgagaatgcc
     1741 gttcacgcgg tgcaacgcgc gagcgtcgct ccgatcgcgg gctgggtctc gcgcgccttc
     1801 gaccgtcgcg agccggcgtc gacgagcgtg tggcgcgcgc ggctgtcggg cctttcccga
     1861 ttgcgcaccg taatcacgat tttctagaat cggctcgtgg tccggaaatc gaagacccag
     1921 cagctgaccg acagcatcct ggggcagcac ctggcgctgt cggtcgcggc ccacctcgcg
     1981 cgcactcagc tcgtgcccga gccgctcgcg gtctacgact cggagcatct gagcgaaatg
     2041 atcgatgtcg tagcgcgcgc gctcgtcagg gtggcgcccc tctacgtgcg cgatgccgga
     2101 tcgggcgagc cgcgcgagct cacggcgttc gagctcgaag gtgcgctgat cgctcgcgcg
     2161 gcgacgctgc tcacgctgaa ggacggccgc agcttctcca gcgcttcgat caagcgcgcg
     2221 gacctgcgcc aggcgatcgt gatcctgaag gcggtcggcg tcgatgagct cgcgcc
//
LOCUS       DASUNT010000308         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_41493, whole genome shotgun sequence.
ACCESSION   DASUNT010000308 DASUNT010000000
VERSION     DASUNT010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41493"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1218)
                     /locus_tag="VFX94_06220"
     CDS             complement(43..1218)
                     /locus_tag="VFX94_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5767812.1"
                     /translation="MQRRNVIVVGAGPVGTVAALACARHGHTVTLLEAQQRIDDSPRA
                     STTQPPTLEMLAELGLIDEYLRVGLVSRTFQFWDRPTRSLIAEFDFARLKGETAYPFV
                     VQTEQHKLANMAIERLRGMANAEVRLGERVTGISQTDDAVDVVTSSRSLHADYVIAAD
                     GGRSTIRKALGIEFEGYTWPERFLVITTKFDFQAALGCCLRTYMADPQEWTNLFKVAG
                     DDLQGRWRAVYNTREDETDEEALSDESVRARLSRIYVPERERDYLHLNLYAVHQRVAK
                     SFRKGRVFLCGDAAHVNNPIGGLGLNCGIHEAWHLSQLLHAVLTGAPVERLDEYETTR
                     RPLNIKYVQEQTVANKKRLEEREPAQREKRFAELRAMADDPQRHKAFLMRASLLERS"
     gene            1244..2191
                     /locus_tag="VFX94_06225"
     CDS             1244..2191
                     /locus_tag="VFX94_06225"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5767813.1"
                     /translation="MKKTAWSLVAMFLLSANALAQQWPQKPIKVIVPFPPGGVTDSLA
                     RITADWLAPRLGQPVVAENRPGASGAIAAELVARADADGYTLFSAATPQLAIVPHVQK
                     VSYDPLKDFAPISIVGTNPFALGCNDKLPVKTLAELVSYAKQRPGQLSFASPGSGSVG
                     HLTMALFLARAGLKMEAVLYKGGGPAMADVVAGHVPCYFGNLNEIIPPAGSGRIRVLA
                     VSGERRAPQLPQVPTVAEQGYPGFRTLTWNGYVAPGATPRDIVERIAREIAAGCKDAA
                     FVERLNKIGVDAVCSTPAEFSQAVREDLATWKEAVQAAK"
     gene            2188..>2506
                     /locus_tag="VFX94_06230"
     CDS             2188..>2506
                     /locus_tag="VFX94_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5767814.1"
                     /translation="MKVAIPDLISPSYFPAIAAVELGFFKKEGIDATLELLYPVTKTY
                     EDLRDGKLDFVGGASHAVLYAFKDWQGAKLLCALAQHMYWFLVVRSDLKAKQGDLNIV
                     RGRK"
BASE COUNT          399 a          849 c          827 g          431 t
ORIGIN      
        1 ctgggtcccc gccttcgcgg ggacgacttc ttcgcacctt ccctaggagc gctccagcag
       61 cgaggcgcgc atcaggaacg ccttgtggcg ctgcgggtcg tcggccatcg cccgcagctc
      121 ggcgaagcgt ttctcacgct gcgcaggctc gcgctcttcc aggcgcttct tgttggccac
      181 cgtctgctcc tgcacgtatt tgatattcag cggacgtctg gttgtttcgt actcatccag
      241 cctctcgacc ggggcgccgg tgagcaccgc gtgcaaaagc tggctcagat gccaggcctc
      301 atggatcccg caattcagcc caaggccgcc gatcgggtta ttcacgtgcg ccgcatcgcc
      361 gcaaaggaac acccgcccct tgcggaagct tttcgccacc cgctggtgca ccgcatacag
      421 gttgaggtgc aggtagtcgc gctcgcgctc gggcacatag atgcgcgaca ggcgcgctcg
      481 caccgactca tcggatagcg cctcctcgtc ggtttcgtcc tcgcgcgtgt tgtacaccgc
      541 ccgccagcgg ccctggagat cgtcgccggc caccttgaag aggttggtcc actcctgcgg
      601 atcggccata taggtgcgca ggcagcagcc gagcgcggcc tggaaatcga acttggtggt
      661 gatgacgagg aagcgctccg gccaggtata gccctcgaac tcgatgccga gcgccttgcg
      721 gatcgtgctt cggcccccgt ccgcggcaat gacgtaatca gcatgcaacg atctgctgct
      781 ggtgaccacg tcgacggcgt cgtcagtctg agaaatgccg gtgacgcgtt cgccgagccg
      841 cacctcggcg ttcgccatgc cgcgcagccg ctcgatggcc atgttggcga gcttgtgctg
      901 ctcggtctgc accacgaacg ggtaggcggt ctcgcccttc agccgcgcga agtcgaactc
      961 ggcgatcagc gagcgggtcg gacggtccca gaactggaag gtgcgcgaga ccaggccgac
     1021 gcgaaggtac tcgtcgatca ggccgagctc ggcgagcatc tcgagcgtcg gcggctgggt
     1081 ggtggaggcg cgcgggctgt cgtcgatgcg ctgctgcgct tcgagcagcg tgacggtatg
     1141 accgtggcgc gcgcaggcca gggccgccac cgtgccgacc gggcccgcgc cgacgacgat
     1201 gacgtttcgc cgctgcattt ttcgctgcga ttatactgag gcaatgaaga aaaccgcgtg
     1261 gtcgctcgtc gcgatgttcc tgctttccgc caatgcgctt gcccagcaat ggccgcagaa
     1321 gcccatcaag gtcatcgtgc ccttcccgcc cggcggcgtt accgattcgc tcgcgcgcat
     1381 caccgccgac tggctcgcgc cgcgcctcgg gcagccggtg gtggccgaga accgcccggg
     1441 cgcgagcggc gcgattgccg cggagctggt ggcgcgcgcc gacgccgacg gctacacgct
     1501 gttctccgcg gcaacgccgc agctggcgat cgtgccccac gtgcagaagg tgtcgtacga
     1561 cccgctcaag gacttcgcgc cgatctcgat cgtcggcacc aatccgttcg cgctcggctg
     1621 caacgacaag ctgccggtga agaccctcgc ggagctggtc agctacgcga agcagcgccc
     1681 ggggcagctc agcttcgctt cgccggggtc gggctcggtc gggcacctga cgatggcgct
     1741 gttcctcgcc cgcgccgggc tgaagatgga agccgtgctc tacaagggcg gcggcccggc
     1801 gatggccgat gtcgtcgccg gccacgttcc ctgctatttc ggcaacctga acgagatcat
     1861 tccgcccgcc ggcagcggcc gcatccgcgt gctggccgtt tccggcgaac ggcgcgcgcc
     1921 gcagctgccg caggtgccca ccgtcgccga gcagggctat ccgggctttc gcacgctcac
     1981 ctggaacggc tacgtagcgc cgggggcaac gccgcgcgac atcgtcgagc gcatcgcgcg
     2041 cgagatcgcg gccggctgca aggacgccgc cttcgtcgag cgcctgaaca agatcggcgt
     2101 cgacgcggtg tgcagcacgc cggcggagtt ttcccaggcg gtccgcgaag acctcgccac
     2161 ctggaaggaa gcagtgcagg cggcgaaatg aaggtcgcga tccccgacct gatctcgcct
     2221 tcgtacttcc ccgccatcgc ggcggtcgag ctcggcttct tcaagaagga aggcatcgac
     2281 gccacgctcg agctcctcta ccccgtaacg aagacctacg aggacctgcg cgacggcaag
     2341 ctcgacttcg tcggcggcgc ttcgcacgcg gtgctctatg cgttcaagga ctggcaggga
     2401 gcgaagctgc tgtgcgccct ggcgcagcat atgtactggt ttctagtcgt tcgttcagat
     2461 ttgaaggcga agcagggcga cctcaacatc gtcaggggaa gaaaaa
//
LOCUS       DASUNT010000309         5159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_567677, whole genome shotgun sequence.
ACCESSION   DASUNT010000309 DASUNT010000000
VERSION     DASUNT010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567677"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VFX94_06235"
     CDS             complement(<1..167)
                     /locus_tag="VFX94_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004542207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX5767815.1"
                     /translation="MSIRTLKPQHETRTRILDAAEELFMQHGFEGTSMRLLTAKAEVN
                     LAAVNYHFGSK"
     gene            complement(208..1323)
                     /locus_tag="VFX94_06240"
     CDS             complement(208..1323)
                     /locus_tag="VFX94_06240"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5767816.1"
                     /translation="MSPHVAANALPEGYVLQEYRIQRVLGVGGFGLTYLALDSNLNLK
                     VAVKEYLPADVAQRGADQTIGPRSPSTAETFGWGKQRFMDESRTVASFRHPNIVRVMR
                     FFEANGTAYMVMEFVDGAPLGEWIKPLRPVSEAQAAALVGPLLDGLDVIHKAGYLHRD
                     IKPGNIYIREDGSPVLLDFGSARQRATELTAVVSPGYAPFEQYHSQGKQGPWSDIYAL
                     AGVLYWMVTGSMPLEAPARVRADEMAAAIQAADRSRFRPEFLAAIDWALAPFEDQRPQ
                     SVAEWREALLGAKAVEAKTEKVQTVPLQSLPAEVLKHAEEQLAEYIGPVAGVVVKRAA
                     AKARDETELYLLLADEIEDKEERKAFVRKTVSISGKP"
     gene            complement(1334..2206)
                     /locus_tag="VFX94_06245"
     CDS             complement(1334..2206)
                     /locus_tag="VFX94_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEX5767817.1"
                     /translation="MGLRLKFNLVLLLVFLIGLGATGYASYDLLHRNARDEVLRNAGV
                     MMEAALSMRGYTIAKVRPLLPYDADKFVPETVPAFAATEVMTLLRKKYPDFSYKEAAL
                     NPTNARNRAVEWEEDIIKAFRDNPVRAEISGIRETLTGRSLYLARPFQIKDPGCLACH
                     TTPEMAPAAMVKMYGSNNGFGWKENEIIGAQIVSVPMGLPMRNANRAFVTFMTSLAVV
                     FAALFVILNIMLTQLIIEPMTRLSEMAEQISRGKPDVPELVTRGDDEVAQLGQAFNRM
                     RRSLQKAISLIEEG"
     gene            2282..4093
                     /locus_tag="VFX94_06250"
     CDS             2282..4093
                     /locus_tag="VFX94_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEX5767818.1"
                     /translation="MAHSAAPTQRLRGNEWKAVATLLPYLWEYKARVVMALACLVIAK
                     LANVAVPLVMKEVVDNLDPSLQIVAVPVALLAVYGVLRFSTTLFQELRDVVFVRVTQR
                     AMRRVALGVFRHLHSLSLRFHLERQTGGMTRDIERGTRGISTLLSYLIFSIIPVILEF
                     ALVAAVLTTRFDWRFAAITFGAVAVYIVFTVLVTEWRMEIRRRANELDSRANTRAVDS
                     LLNYETVKYFGNEEFEARRYDEQLHKYEDAATQNEASLGILNIGQSLIIAMAVTALML
                     LVAQGVAANSFTIGDLVLVNGLLIQLYIPLNFLGMVYREIKQALLDMDRMFRLLSEHR
                     EVEDRPGAPELPAGPATVEFRHVEFSYEKARQILFDVSFEIPAGQRVAVVGHSGSGKS
                     TLARLLYRFYDVSGGSIRINQLDIRDARQSSLRAAIGIVPQDTVLFNDTILYNIRYGR
                     PGASDEEIYEAARAAHIHDFIESLPRKYETQVGERGLKLSGGEKQRVAIARALLKNPR
                     ILIFDEATSALDSRAEKAIQAELERIAQGRTTLVIAHRLSTVMDAHQILVLAQGRIIE
                     RGTHLQLLDAKGEYARMWALQQQQAQAQELLESTAAE"
     gene            4274..5131
                     /gene="pbpG"
                     /locus_tag="VFX94_06255"
     CDS             4274..5131
                     /gene="pbpG"
                     /locus_tag="VFX94_06255"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF008668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HEX5767819.1"
                     /translation="MQRKLHPLEGIVAICALVLVLFVSVGFAQAQDVRKIALRSSSAL
                     VQHAGSGEVVYGKNTDVVVSIASITKLMTAMVVLDYGLDLDEEIVLSKEDAVRMKGSR
                     SRLRTGLKLSRGELLLLSLMASDNRAAAALGHSYPGGLEAFVDAMNVKAALLDMEETR
                     FVEPTGLSPANVSTASDLAKMVRAAHEYPLIREYSTKTRATVSASGRPLSYGNTNNLV
                     RSKYWDIELSKTGYISEAGRCLVMQVRLADKDLIVVLLDSWGKQSRIGDANRIRKWLE
                     SGAPTGRSS"
BASE COUNT          910 a         1631 c         1662 g          956 t
ORIGIN      
        1 tccttcgagc cgaagtggta gttgaccgcg gccaggttga cctcggcctt cgcggtgagc
       61 aggcgcatcg aggtgccttc gaagccgtgc tgcatgaaca gctcttcggc ggcgtcgagg
      121 atgcgggtgc gggtttcatg ctgcggcttg agcgtgcgga tggacatgag ccatgagaaa
      181 gaaattcaaa cgaacgtttg aaatctatca tggcttgcct gaaatggaaa ccgttttgcg
      241 aacgaaggct ttgcgctctt ccttgtcttc gatctcgtcg gcgagcagca agtagagctc
      301 ggtctcgtcg cgcgccttgg cggcggcgcg cttcaccacc accccggcca ccggcccgat
      361 gtattccgcg agctgctctt cggcgtgctt cagcacctcc gccggcaggc tctgcagtgg
      421 tacggtttgc accttttccg ttttcgcttc aacggccttg gcgccgagca gggcttcgcg
      481 ccattcggcg accgattgcg gccgctggtc ctcgaagggc gcaagcgccc agtcgatcgc
      541 cgccaggaac tcgggccgga agcggctgcg gtcggcagcc tggatggcgg cggccatctc
      601 gtcggcgcgc acgcgcgccg gcgcctcgag cggcatcgag ccggtcacca tccagtagag
      661 cacgcccgcg agcgcgtaga tgtcgctcca cggtccctgc ttgccctggc tgtggtactg
      721 ctcgaagggc gcatagcccg gcgagaccac ggcggtaagc tcggtggcgc gctggcgcgc
      781 cgagccgaag tcgagcagca cggggctgcc gtcctcgcgg atgtagatgt tgcccggctt
      841 gatgtcgcga tgcaggtagc ccgccttgtg gattacgtcc aatccgtcga gaagcggacc
      901 gacgagcgct gcggcctgcg cttccgacac gggtcggagc ggcttgatcc actcgccgag
      961 cggtgcgcca tcgacaaatt ccatgaccat gtacgcggtg ccgttcgctt cgaagaaacg
     1021 catcacgcgc acgatgttcg ggtggcggaa ggacgctacg gtgcgcgatt cgtccatgaa
     1081 gcgctgcttg ccccagccga acgtctcggc agtggaaggc gagcgcggtc cgatggtctg
     1141 gtcggcaccg cgctgcgcga cgtcggccgg caggtactcc ttgaccgcaa ccttgaggtt
     1201 gagattggag tcgagcgcga ggtacgtcag cccgaagccg cccacgccga gcaccctctg
     1261 gatgcggtac tcctgcagga cgtagccttc cggcagcgcg ttcgcggcga cgtgcgggct
     1321 cattgaccct cgtctaccct tcctcgatca ggctgatggc cttctgcagg ctgcggcgca
     1381 tccggttgaa ggcctggccg agctgcgcca cttcgtcgtc tcccctcgtg acgagctcgg
     1441 gcacgtcggg cttgcctctg gagatctgct cggccatctc ggaaagccgc gtcatcggct
     1501 cgatgatgag ctgggtcagc atgatgttca agatgacgaa aagcgccgcg aagacgaccg
     1561 cgagcgaggt catgaacgtg acgaaggcgc ggttggcgtt gcgcatcggc agccccatcg
     1621 gcaccgagac gatctgcgcg ccgatgatct cgttctcctt ccagccgaag ccgttgttcg
     1681 agccgtacat cttgaccatc gcggcgggcg ccatttccgg cgtggtgtgg caggcgaggc
     1741 accccgggtc cttgatctgg aaggggcgcg cgagatagag cgagcgcccc gtcagcgtct
     1801 cgcggatgcc tgaaatttcg gcgcggaccg gattgtcgcg aaacgccttg atgatgtcct
     1861 cctcccattc caccgcgcgg ttgcgcgcgt tggtgggatt gagcgcagct tccttgtacg
     1921 aaaagtcggg gtacttcttg cgcagcaggg tcatgacctc ggttgcggcg aacgccggca
     1981 cggtttcggg cacgaatttg tctgcgtcgt agggcagcaa cggccggact ttggcgatgg
     2041 tgtatccgcg catcgagagc gccgcttcca tcatcacgcc ggcgttacgc aagacctcgt
     2101 cgcgcgcgtt acggtgcagg agatcgtacg acgcgtaccc ggtggcgccg agcccgatca
     2161 agaacacgag gagcagcacg aggttgaact tgagccttag gcccatgacc tttctccctt
     2221 ggcggcaact atacgtaact cgccggctgc ctgatgatcg agattgcgat aatccgggca
     2281 tatggcacac agcgccgctc cgacacagcg attgaggggt aacgaatgga aggcggtagc
     2341 caccctcctt ccttaccttt gggaatacaa ggcacgggtg gtcatggcgc tcgcctgcct
     2401 ggtgatcgcc aagctggcga atgtcgcagt gccgctcgtg atgaaggagg tcgtggacaa
     2461 cctcgacccg agcctgcaga tcgttgcggt gccggtcgcg ctgctcgctg tctacggggt
     2521 gctgcggttc tcaaccaccc ttttccagga gctgcgcgat gtcgtcttcg tgcgtgtcac
     2581 gcagcgcgcc atgcggcgcg tcgcgctcgg cgtcttccgg cacctgcaca gcctgtcgct
     2641 tcgcttccac ctcgagcgcc agacgggtgg catgacgcgc gacatcgagc gcggcacgcg
     2701 cggcatctcc acgctgcttt cgtacctgat tttctccatt atcccggtga tcctcgagtt
     2761 cgctctcgtc gccgcggtgc tgacgacgag attcgactgg cgcttcgccg cgatcacctt
     2821 tggcgcggtc gcggtgtaca tcgtgttcac ggtactggtt accgagtggc gcatggagat
     2881 ccgccgccgc gcgaacgagc tcgattcgcg cgccaatacg cgcgcggtcg acagcctgct
     2941 caactacgag acggtcaagt acttcggcaa cgaggagttc gaggcgcgcc gctacgacga
     3001 gcagctccac aagtacgagg acgcggcgac gcagaacgag gcctcgctcg gcatcctcaa
     3061 catcgggcag agcctcatca tcgcgatggc ggtaactgcg ctgatgctgc tggtggcgca
     3121 gggcgtggct gcgaacagct tcacgatcgg ggatctcgtc ctggtgaacg ggctgctgat
     3181 ccagctctac atcccgctca acttcctggg aatggtgtac cgcgaaatca agcaggcgct
     3241 gctcgacatg gaccggatgt ttcgcctcct ttccgagcac cgcgaggtcg aggaccggcc
     3301 aggcgctccc gagctgccgg ccggacccgc aacggtcgaa ttcaggcacg tcgagttctc
     3361 ctacgaaaaa gcgcgccaga ttctcttcga cgtgagtttc gagataccgg cgggccagcg
     3421 cgttgccgtc gtgggtcatt ccggctccgg caagtcgacg ctcgcgcgcc tgctctaccg
     3481 gttctacgac gtgtcaggcg ggtcgataag aatcaatcag ctcgatatca gggatgcgag
     3541 gcagagcagc ctgcgcgcgg cgatcggcat cgtgccgcag gacacggtgc tcttcaacga
     3601 cacgatcctt tacaacatcc gctatggccg gccgggggcg agcgacgagg agatctacga
     3661 ggccgcgcgc gccgcgcaca tccacgattt catcgagtcg ctgccaagaa aatacgagac
     3721 tcaggtgggc gagcgcggtc tcaagctctc ggggggcgag aagcagcgcg tcgcgatcgc
     3781 ccgtgcattg ctgaaaaacc cgcgcatcct gatcttcgac gaagcgacct cggcgctcga
     3841 ctcgcgcgcc gagaaggcga tccaggcgga gctcgagcgc atcgcccagg gtcgcaccac
     3901 gctcgtgatc gcgcaccgac tgtcgactgt gatggacgcc caccagattc tcgtgctcgc
     3961 tcagggccgc atcatcgagc gcggaacgca cctgcaactg cttgacgcta aaggcgaata
     4021 cgcccgtatg tgggctctgc agcagcaaca ggcgcaagca caggagctcc tcgagagcac
     4081 tgccgccgag taacccaaac ccgacgccgc ggcccgacag tggagaatct cacgccgatt
     4141 cttgaaagtt cgtgctagcc cctgtttggt ttgcagtttt ttcaaaccgt aggctatatt
     4201 gcgacgactc atgcgccgct gatgtatagg cgcctaataa aagccactgc ggatagaggg
     4261 ggaagattca acaatgcaaa gaaaactgca tccgctcgag ggcatcgtcg ccatctgcgc
     4321 cctcgtgctc gtgctgttcg ttagcgtcgg cttcgcgcag gcccaggatg tacgcaagat
     4381 cgcattgcgc tcctcttcgg cgctggtgca gcacgcggga agcggcgaag tggtctacgg
     4441 caagaacacc gatgtagtgg tttccattgc ttcgatcacc aagctcatga cggcaatggt
     4501 ggtcctcgac tacggtctcg acctcgacga ggagatcgtg ctctcgaagg aagacgcggt
     4561 acggatgaag ggctcgcgct cccggctgcg cacggggctc aagctctcgc gcggcgagct
     4621 tctgctgctc tcgctgatgg cttccgataa ccgtgccgcc gcggcgctcg gccacagcta
     4681 tcccggcggc ctcgaagcct tcgtcgatgc aatgaacgtg aaggcggctt tgctcgacat
     4741 ggaggagacg cgattcgtcg agccgaccgg gctttcgccc gccaatgtct ccaccgcgag
     4801 cgacctcgcg aagatggtgc gcgcggcgca cgagtacccg ctgatccggg aatactcgac
     4861 caagacgcgg gccaccgtca gcgcgtcggg gcggcccctc agctacggca acaccaacaa
     4921 tttggtacgc tcgaagtact gggacatcga gctctccaag accggctaca tcagcgaggc
     4981 cgggcgctgc ctcgtgatgc aggtccgcct cgccgacaag gacctgatcg tggtgctgct
     5041 cgactcgtgg ggcaagcaat cgcgcatcgg ggacgccaac cgcatccgca agtggctcga
     5101 gagcggcgcg ccgaccggcc gctcgagcta aagcgctttc gcgacgacgt agtggcggg
//
LOCUS       DASUNT010000310         3217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_217861, whole genome shotgun sequence.
ACCESSION   DASUNT010000310 DASUNT010000000
VERSION     DASUNT010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217861"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1300)
                     /locus_tag="VFX94_06260"
     CDS             complement(95..1300)
                     /locus_tag="VFX94_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HEX5767820.1"
                     /translation="MPLEKPSRPPGLRRLLADSGASSAAAAFVAFLFAASGPVAIILS
                     VGARGGLTESDIASWIFAAFCLNSVISIGFSLAYRQPLVFLWSIPGAVLVGPALGHVT
                     FPEVIGAFLATGALMLLLGLSGWVRRAMDAVPMPIVMAMVAGVFLRFGVDLVLAFRSE
                     LWIALPMTAVFFLTTILRVDKYLPPLIAALVTGGVAIAALGAFNPPPGVLFAFASPNL
                     YVPKFSAAAMVELVVPLAITVLAVQNAQGVAILSTNGHKAPVNAITAACGAGSIVTGF
                     FGAVSTCLTGPVNAVLSGTGEKSTQYTGALFFCVLALAFGSLAPFFTRLLVATPPAFI
                     ATLAGLAMLRVLQSAFTASFGGRFSFGALVCFLVTVADVAIFNIGAPFWGLVLGLAGS
                     WLLERPDRR"
     gene            complement(1304..1960)
                     /locus_tag="VFX94_06265"
     CDS             complement(1304..1960)
                     /locus_tag="VFX94_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEX5767821.1"
                     /translation="MRALSLLETRVLGTLLEKQHTVPDSYPLTLNALVSGCNQKSSRS
                     PVMEATEAEVQASVGSLKAANLVIETSGGRVARYSHNIERVLQVPSQSAALLATLMLR
                     GPQTAGELRINTERLHSFADISAVQGFLDELAARPAGALVVQLPRQPGSRENRWAHLL
                     SGASAVEDKKPETEKKADADVSIGEMAALKANLARLEGEVAALKALLARVCKELGIGA
                     "
     gene            2031..>3217
                     /locus_tag="VFX94_06270"
     CDS             2031..>3217
                     /locus_tag="VFX94_06270"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX5767822.1"
                     /translation="MAYLVGIDIGGTFTDCAIVDRAGKLLTTKVPSTPEDFSRGMLNA
                     LGAGAQALGLGLEPFCRDIAFLSHGTTVGTNTIIQKRGAKVGLITTKGHEDAIHIMRG
                     SRGYGGRDIRKVVHFPETSKPQPIVPKRLIRGVSERVDCFGEIVVALNTEETEGAIRE
                     LLAEGVEAIAICLLWSFRNPQHEMAVKKLVQRAAPSLFVTCSHDIAPKWGEYERVTAT
                     ALNAYLGPVMSGYLDRLNSSLKKLGYGHGLQITQCGGGTVPVERAGEAPLLTLDSGPV
                     SGVTASMFLGAAMGEKNVITTDMGGTSFDVSIIYDGKPAYSFIANTDQYDYFLPKVDL
                     QAIGAGGGSLVRVRPETGTMSVGPDSAGAFPGPVCYGRGGQVPTVTDAQLVLGYLDPD
                     NFAG"
BASE COUNT          584 a         1085 c         1074 g          474 t
ORIGIN      
        1 ctccgaggta gttaatttaa ctacgttttc caaggtccgc gcaatgcaaa aaagcacaac
       61 ggccgggccg attccgccgc tcctccgccc gtagctaccg ccggtcggga cgctcgagca
      121 gccaggaccc tgcgagccca agtaccagcc cccagaaggg ggcgccgatg ttgaagatcg
      181 cgacgtcggc gacggtaacc aggaagcaca ccagcgcgcc gaagctgaac ctcccgccaa
      241 aggacgcggt gaaggcgctt tgaagcacgc gcagcatcgc caggccggcg agcgtggcga
      301 tgaatgccgg cggcgtggca acgagcagcc gggtaaagaa cggcgcgagc gacccgaacg
      361 cgagcgcgag cacgcagaag aagagcgcgc cggtgtattg cgtcgatttt tctcccgtgc
      421 cggagaggac ggcgttcacc ggacccgtca ggcaggtcga aaccgcgccg aagaacccgg
      481 tgacgatcga gcccgcgcca caggcagcag taatggcgtt caccggcgcc ttgtggccat
      541 tggtggacag aatggcaacg ccttgggcgt tctgcacggc gagcacggtg atcgcgagcg
      601 gcacgaccag ttccaccatc gctgccgcag agaacttcgg cacgtacaag ttgggtgatg
      661 caaacgcgaa caacacgcca ggcggcgggt tgaatgcccc gagggctgcg atcgccacgc
      721 cgcccgtgac cagggcggcg atcagaggcg gcagatactt gtccacccgc agaatcgtcg
      781 tcagaaagaa caccgccgtc atcggcagag cgatccagag ctcgctgcgg aaagcgagca
      841 ccaagtccac gccgaagcgc aggaacaccc ccgccaccat ggccatcacg atgggcatgg
      901 gcaccgcatc catcgcgcgg cgcacccagc ccgagagccc gaggaggagc atgagcgctc
      961 cggtagcgag gaaagcgccg atcacctcgg gaaacgtgac gtgtccgagc gccggcccga
     1021 ccagcaccgc gcccgggatg ctccacagga agacgagcgg ctggcggtac gccagcgaaa
     1081 acccgatgga tatgacgctg ttcaggcaga acgcggcgaa gatccaggag gcgatgtccg
     1141 actcggtcag cccgccgcgc gcgccgaccg aaagaatgat cgccaccgga ccactcgccg
     1201 cgaagaggaa cgcgacgaag gccgctgccg cgctggacgc gcccgagtcg gcgaggaggc
     1261 ggcgcaggcc cggcggccgc gacggtttct ccagcggcat cggtcaggcg ccgatgccga
     1321 gctctttgca gacgcgggcg agcagcgcct tgagcgcggc cacctcgccc tcgagccgcg
     1381 ccaggttggc cttcagcgcc gccatctcgc cgattgaaac gtcggcgtcg gccttctttt
     1441 cggtctcagg cttcttgtct tcgaccgccg acgctccgct cagcaggtgg gcccagcggt
     1501 tttcgcgcga gcccggctgg cgcggcagct gcacgacgag cgctccggcc ggccgggccg
     1561 cgagctcgtc caggaagccc tgcaccgccg aaatgtcggc gaagctgtgc aggcgctcgg
     1621 tattgatgcg cagctcgccc gccgtctgcg ggccgcgcag catcagcgtg gcgaggagcg
     1681 ccgccgactg cgacggcacc tggagcacgc gctcgatatt gtgcgagtag cgcgcaaccc
     1741 tgccgccgct cgtctcgatc accaggttgg cggccttcag gctgccgacg ctcgcctgca
     1801 cttccgcctc ggtcgcttcc attaccggcg agcggctgct cttctggttg cagccggaga
     1861 cgagcgcgtt gagcgtaagc ggatagctgt cgggcacggt gtgctgcttt tcgagcagcg
     1921 tgccgagcac gcgggtctcg agcagggaaa gtgctctcat catcgggacg ctgttttacc
     1981 acgaagcact gcgcaacccg cgccgctgcg ctacgatccg gcgctccggc atggcctacc
     2041 tcgtcggcat cgacatcggc ggcacgttca ccgactgcgc aatcgtcgat cgcgccggaa
     2101 agctgctcac caccaaggtg ccgtcgacgc ccgaggattt ttcgcgcggc atgctgaacg
     2161 cgctcggcgc cggcgcgcag gcgctcggcc tgggcctgga gcccttctgc cgcgacatcg
     2221 cgttcctctc gcacggcacc acggtcggca cgaacaccat catccagaag cgcggcgcga
     2281 aggtgggcct catcacgacg aaaggccacg aggacgcaat ccacatcatg cgcggctcac
     2341 gcggttacgg cgggcgcgac atccgaaagg tcgtgcactt ccccgagacc tccaagccgc
     2401 agccgatcgt tcccaagcgc ctgattcgcg gggtctccga gcgcgtggac tgcttcggcg
     2461 agatcgtggt tgcgctgaat acggaagaaa ccgaaggcgc gatccgcgag ctcctcgccg
     2521 agggcgtgga ggcgatcgcg atctgtctgc tttggtcgtt ccgcaacccg cagcacgaaa
     2581 tggcggtcaa gaagctggtg cagcgtgccg cgccgtcgtt gttcgtcacg tgttcccatg
     2641 acatcgcgcc aaagtggggc gagtacgagc gcgtcaccgc gacggcgctg aatgcgtatc
     2701 tcggcccggt aatgtcgggc tatctcgaca ggctgaacag ctctttgaag aagctcggct
     2761 acggccatgg gctgcagatc acccagtgcg gtggcggaac ggtgccggta gagcgcgcgg
     2821 gtgaagcgcc tttgttgacg ctcgattccg gccccgtgtc gggcgtgacg gcgtcgatgt
     2881 ttctcggcgc ggcgatgggc gagaagaacg tcatcaccac cgacatgggc ggcacgtcgt
     2941 tcgacgtttc catcatctac gacggcaagc cggcctactc cttcatcgcg aacacggacc
     3001 agtacgatta cttcctgccc aaggtcgacc tgcaggccat cggcgccggc ggcggcagcc
     3061 tggtgcgggt gcggcccgag accggcacca tgagcgtcgg ccccgacagc gccggcgcct
     3121 tccccggccc ggtgtgctac gggcggggcg gtcaggtgcc gacggtgacc gacgcgcagc
     3181 tcgtcctcgg ctatctcgat cccgacaact tcgccgg
//
LOCUS       DASUNT010000311         4578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_655938, whole genome shotgun sequence.
ACCESSION   DASUNT010000311 DASUNT010000000
VERSION     DASUNT010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655938"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VFX94_06275"
     CDS             complement(<1..475)
                     /locus_tag="VFX94_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5767823.1"
                     /translation="MGTMPGKNLKTVLFAEVSGRDEFVRAAGFVTGWNGISDCVERLQ
                     RTARSSGGRVVKVIGSELMMLFDTPDAAAAAASSMHAAVNALPPVSGARLSVKVGYHA
                     GPVIEGNDDLLGDTVKLVEALLRQARAGQTMTTPETAQLLNKKSCSYSGEVWAGAL"
     gene            679..843
                     /locus_tag="VFX94_06280"
     CDS             679..843
                     /locus_tag="VFX94_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767824.1"
                     /translation="MKLFRKLRGLLEYRLFYAALLPTLIILIAAGVVLAGGEGAAALT
                     TPAIPIYKAS"
     gene            1395..1781
                     /locus_tag="VFX94_06285"
     CDS             1395..1781
                     /locus_tag="VFX94_06285"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX5767825.1"
                     /translation="MRALALKQLRTVAMLAALGACAQPPAPQVEPARADGVTPGTIGV
                     VVSDEQTGVVVTALGNDGAAAHSGVQVGDVVLSCNGERVSTSRHFNRLVVGTPPGTRL
                     RLELLREGTVRTLEVPVEQLDLAPRG"
     gene            complement(1778..3103)
                     /locus_tag="VFX94_06290"
     CDS             complement(1778..3103)
                     /locus_tag="VFX94_06290"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5767826.1"
                     /translation="MVWVTGALMLGIAVVALATYYAALQQIGTLFDAALTQIAQAVHL
                     REDWAEAGTVRIAQEEVAFAVRAYDDGGRVFFESGLPALPPDAPKTLDPGFSVVATPD
                     GPWRVYTHVTPEGVVQVAQAEAVRDALARELSLRMLLPELLFVPVLALLVAWILKRAL
                     APLDETSRRVRERNAARLDPLPTAGVPRELTALVAQVNALLGRLAASLESERRFVADA
                     AHELRSPVAALALQIQLAERAVTEAGRSAALAELKKGVARAGRLVQQLLDLARLEPGI
                     PSEVPGRVDLAQLMRDVIGSFAARADAQDVDLGAEAPLSVLVTGRSSELRSLVANLID
                     NALRYAPPQTAVTVSLREASGAVELAVEDAGPGIPPEQRERVMRRFQRVDGDPSAGSG
                     LGLPIAKAIVERHNGTLALHEARPGRQPPGLRVQVRLPAAQRAQTALAA"
     gene            complement(3124..3786)
                     /locus_tag="VFX94_06295"
     CDS             complement(3124..3786)
                     /locus_tag="VFX94_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5767827.1"
                     /translation="MRVLLVEDDPMIGRAVADGLQGEGHAVDLVFDGAAAELALLHGP
                     YDLAVLDLGLPRKDGFEVLKSLRRARIEVPVLIITARDAVADRIAGLDHGADDYMVKP
                     FELDEFLARARAVVRRRAGRVSPEITYGALTLDPVKRAVSLRGTPVELSSREFAVLEA
                     LMREPGAVVSRSKLEDSVYGWGEEVGSNSVEVHLHHLRRKLDPTLIRNVRGVGYRVAR
                     LD"
     gene            complement(3926..>4578)
                     /locus_tag="VFX94_06300"
     CDS             complement(3926..>4578)
                     /locus_tag="VFX94_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5767828.1"
                     /translation="LLLHTAAEFAQRGYAVLMPLRRGFGATGGPYAENPGMCSNADYA
                     RGERAAAADVLAAYAFARKLPYVDPERVILAGQSAGGVAALYAASQSPPGLQAVLAFA
                     AGRGADPDNPGVPCAAPRLAEVFEDMGRRLKVPVLLYYAKNDLFFGPDATQGWFTRFK
                     AGGARAEYVLQPPFGANGHYIFTDAAGVALWLPAVENFLRRHGIPFSPSTADSRAA"
BASE COUNT          738 a         1574 c         1563 g          703 t
ORIGIN      
        1 cgagcgctcc ggcccacacc tcgcctgaat aggagcacga cttcttgttg agcagctgcg
       61 ccgtctcggg cgtggtcatc gtctggcctg ctcgcgcctg gcgcaggagc gcctccacaa
      121 gctttaccgt atcgccgaga agatcgtcgt tgccctcgat cacgggaccg gcgtgatagc
      181 cgactttgac cgagagccgc gctcccgaca ccgggggcag cgcattcacc gccgcgtgca
      241 tgctggaagc cgcggccgcg gccgcatcgg gcgtatcgaa cagcatcatg agctcgctgc
      301 cgatgacctt caccactctg ccgccggagg atcgcgcggt gcgctgcagg cgctcgacgc
      361 agtcggaaat gccgttccac cccgtgacga aaccggccgc ccgcacgaat tcatcccggc
      421 cgctcacttc ggcgaacagc acggtcttca ggttttttcc tggcatcgtg cccattggcg
      481 tgtctctgcc ctgaattctt ttccccgttt gcgcgcctgc gtaaggcaaa aaatcacccg
      541 cggtgaaaga ggcatcgtcg ttcgcgcacg accatcccgg cgggatggtg cataactcac
      601 atccaattgt ggccccgctt cactgacggc atggcgcttg gcgccttatc gttcgaacgc
      661 aacggcaagg agggcgctat gaaactcttc agaaagcttc gcggtttgct cgagtaccgg
      721 ctgttctacg ccgcgctcct tcccacgctg atcatcctca tcgcggccgg tgtggtgctc
      781 gccggtggag agggcgcggc ggcgctcacg acgcccgcga tcccgatcta caaggcaagc
      841 tagggcatgc cgtccgtggc tacgaccgga cggcaacccg cgcgcacaga cgctcggcta
      901 cttctgcaag gaatgcccta actcgtcctg caacgcctcg gccttcctta gcgcttcttc
      961 agcacgccgg cgggccatcg ccccgatttg cgccagaagc aacgagtacg gatactcggc
     1021 gcgacacgcc aggcaacgca tcatgtccat cttttcgcgt gagcgaatga aatgctcaca
     1081 gcggcagcgc tggcactgag cgatggtggt gcctatctgt atctcacgcg gtctcgtgaa
     1141 gtttctccgt tcctccatgc ctcctcgtca agtcatggga actagtttgg gtcttcacgg
     1201 aacacaggtc tgtagcgtcc ggcaaccggg tgatgtgagc aattcactgg tttggggctt
     1261 tacgggcgtg ccaaaccgtg gttaagagac gaggggcgac cctccaaagc atagaaatgt
     1321 caacgactta gcttagaacc cctccaattg tgaaggcgca tgacgcgttt attgaggacg
     1381 gcacccgaga tgacatgcgg gccctcgcct tgaagcagct gcgcacggtg gcgatgctgg
     1441 ccgcgctcgg ggcctgcgcc cagccgcccg cgccgcaggt ggagcctgcc cgtgccgatg
     1501 gcgtgacccc cgggacgatc ggcgttgtgg ttagcgacga gcagaccgga gtcgtggtga
     1561 ccgcgctcgg caacgacggt gcggccgcgc acagcggcgt ccaggtgggc gacgtggtgc
     1621 tgagctgcaa cggcgagcgc gtcagtacgt cgcgccactt caatcgcctg gtggtcggca
     1681 cgccgcccgg cacccggctg cggctcgagc tgctgcgcga aggcaccgtg cgcacgctcg
     1741 aggttccggt cgagcagctc gacctcgcgc cgcgcggcta agcggcaagc gcggtttgcg
     1801 cccgctgcgc cgccggaagg cggacctgta cccgcagacc tggcggctgc cgtcccggcc
     1861 gcgcctcgtg gagcgcgagg gtgccgttgt ggcgctcgac gattgccttg gcgatcggca
     1921 ggccgaggcc gctgcccgcg ctcgggtcgc cgtcgacgcg ctgaaagcgc cgcatgacgc
     1981 gctcgcgctg ctcgggcgga atgccgggcc cggcgtcctc gacggcaagc tcgaccgcgc
     2041 cggaagcctc gcgcagcgac acggtcacgg ccgtctgcgg cggcgcgtag cgcagtgcgt
     2101 tgtcgatgag gttggcgacc agcgagcgga gctccgagga gcggccagtg acgagcaccg
     2161 aaagcggcgc ctcggcgccg aggtcgacgt cctgggcgtc ggcgcgcgcg gcaaagctgc
     2221 cgatcacgtc acgcatgagt tgcgcgaggt ccacccgccc gggaacctcg gacgggattc
     2281 cgggctccag gcgcgcgaga tcgagaagct gctgcaccag ccgcccagcg cgcgccacgc
     2341 ctttcttgag ctccgccagc gcggcggagc ggcccgcctc ggtaaccgcg cgctcggcga
     2401 gctggatctg gagcgcgagc gcggcgaccg gcgagcgcag ctcgtgcgcg gcgtcggcga
     2461 caaagcggcg ctcggactcg agcgatgcgg cgaggcgccc gagcagggcg ttgacctgcg
     2521 cgacgagcgc ggtgagctcg cggggaacgc cggcggtcgg caggggatcg agccgcgccg
     2581 cgttgcgctc gcgcacacgc ctggaggtct cgtcgagcgg cgcgagcgcg cgcttcagaa
     2641 tccaggcgac gagcagcgcg agtaccggga cgaagagcag ctcgggcagc agcatgcgca
     2701 gcgacagttc gcgcgcaagc gcatcgcgca ccgcttcggc ttgcgccacc tggaccacgc
     2761 cctcgggcgt cacgtgcgta tacacgcgcc acggtccgtc aggcgtcgcc accacgctga
     2821 aacccggatc gagcgtcttc ggcgcgtcgg gcggcagcgc cggcaggcca ctctcgaaga
     2881 aaacccgtcc gccgtcgtcg tacgcgcgca ccgcgaaggc gacctcttcc tgcgctatgc
     2941 gcacggtgcc ggcttcggcc cagtcttccc gcagatgcac cgcctgcgcg atctgcgtaa
     3001 gcgccgcatc gaacagcgtg ccgatctgct ggagcgccgc gtaataggtc gccagcgcga
     3061 ccacggcgat gccgagcatg agcgccccgg taacccaaac cacgagctgg cgccggatcg
     3121 atgtcaatcg aggcgggcga cgcggtaacc cacgccgcgc acgttgcgga tgagcgtcgg
     3181 gtcgagcttg cgccgcaggt ggtgcaggtg cacttcgacc gagttcgagc cgacttcctc
     3241 gccccagccg tagacactgt cctcgagctt gctgcgcgac accaccgcgc cgggctcgcg
     3301 catcagcgcc tcgagcaccg cgaattcccg cgaagagagc tctaccggag tgccgcgaag
     3361 gctcaccgcg cgcttcactg ggtcgagcgt gagcgcgccg taggtgatct cgggcgacac
     3421 gcggcccgcg cgccggcgca cgacggcgcg cgcgcgcgcg aggaattcat cgagctcgaa
     3481 cggcttcacc atgtagtcgt cggcgccgtg gtcgaggccg gcgatgcgat cggcaacggc
     3541 gtcgcgcgcc gtgatgatga ggacgggtac ctcgatgcgc gcgcggcgca ggctcttcag
     3601 cacctcgaag ccgtccttgc gcggcaggcc caggtccagc accgcgagat cgtatgggcc
     3661 gtgcagcagc gcgagctcgg cggcggcgcc gtcgaatacg agatccacgg cgtggccttc
     3721 gccttgaagt ccatcggcca ccgcgcggcc gatcatggga tcgtcttcaa ctaagaggac
     3781 tcgcatggcc cgagtatagg tcgctccatc tttaaggcgc ttttaagcct agggagttca
     3841 cgctcttccg actcgcgcga aggcatgaac tcgcgtctcc tcctcgccgt actcctgctg
     3901 tgcggcagcg cagcagcgag cgcggtcagg ccgctcggga gtccgctgtt gacgggctga
     3961 acggaatgcc gtggcggcgc aggaagttct ccactgcggg aagccataac gcaacgcccg
     4021 ccgcgtcggt gaagatgtag tgcccgttcg cgccgaaagg cggctgcagc acgtactcgg
     4081 cgcgcgcgcc tcccgccttg aagcgggtga accagccctg cgtagcgtcc gggccgaaga
     4141 agagatcgtt cttcgcgtag tagagaagca ccggcacctt gagcctcctg cccatgtcct
     4201 cgaacacttc ggccagccgc ggcgctgcgc agggcacgcc agggttgtcc ggatcggcgc
     4261 cccggcccgc ggcgaaggcg agcaccgcct gcagccccgg cggggactgc gaagcggcat
     4321 agagcgcggc gacgccgccc gcggactgcc cggcgaggat cacgcgctcc gggtcgacat
     4381 acggcagctt gcgggcgaaa gcgtaagcgg ccagcacgtc ggcggccgcg gcgcgctcgc
     4441 cgcgcgcata gtcggcgttg ctgcacatcc ccgggttctc ggcgtagggc ccgccggtcg
     4501 cgccgaagcc gcggcgcagc ggcatgagga ccgcatagcc gcgctgcgcg aactccgccg
     4561 cggtatggag aagcaggg
//
LOCUS       DASUNT010000312         4290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1182150, whole genome shotgun sequence.
ACCESSION   DASUNT010000312 DASUNT010000000
VERSION     DASUNT010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182150"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1751)
                     /locus_tag="VFX94_06305"
     CDS             complement(<1..1751)
                     /locus_tag="VFX94_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5767829.1"
                     /translation="MSAVSLQVQFDPASARFGASRSQKRLEDERLLRGSGLFSDDREF
                     PGQAWMVLVRSPHAHARISSIDLSEALKSKGVLVAWSMQELRADGVKHIPFPPLFKRA
                     DGSPMAAPPRTPLAEGNVLYVGQPVAAVVAETRVQAQDAAELVTVEYEELPCVVDPRK
                     AVEADAPVLWEQAGGNVAAEAVYGNKEAAESAFSAAAHVTRIELHNQRLNAMALEPRC
                     AIGVQENGRTTLYTQCQTPTAARELLGAVFGEPPANFRLVNGDIGGGFGMKTGLTPED
                     ALVCYAARKLGRPVKWRADRAEEFLAAHMARDQHYRAALALDREGRILALRMEMLANI
                     GAVPVGSSAMIPLAMVAKVATSAYRVPVVDYRVKGVLTNTMATGAYRGAGRPEGNYLI
                     ERLLEKSAREMGIDRVELRRRNLLRADELPYRTHVGDTYDGGDFGRMLQGVLHRAKWD
                     DFPARKEDSSRLGKLRGRAVSVYLEWTGALPTETVDIEIDAEGVVSVFSGTQAMGQGL
                     ETTYTQLIEEVLGVPPEKIRIVQGDTDRANGVGSVGSRSAFVGGSAVVAAGRKAIARG
                     KELAADALEAAPADIEYR"
     gene            complement(2007..4259)
                     /locus_tag="VFX94_06310"
     CDS             complement(2007..4259)
                     /locus_tag="VFX94_06310"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5767830.1"
                     /translation="MSRWLLNPAMITAGTIAIVVALFAISPAMFEAIELNWLDLRFRA
                     RGPIKPGPAVVIAAIDEKSLESEGRWPWPRARIAALIDALARDDAKVVGFDVMFSEPE
                     HDAQLATLADELERTINSLNINNAKLHEIVRRNRVAGEGDQLLARSLKASAAPVVLGY
                     FFHMDESGVGYKLDPAEVARRVDVIAGSKYPLVYVDRDAGALPFVKAYAPQTSLASFT
                     SAAASSGFFSVVADLDGVVRWMPLVVQAGEDLFPPLAVLCVWHYLGKSALAVRAGGRY
                     GAEGVRIGERVVPTDEAGQLLINYRGPAGTFPTYSAGDILAGRLPPGTFAEKIVIVGA
                     TAIGIGDIRTTPFGPLYPGPEVHATVADNILAGDFMQRPRWSKVFDLLAIVALGLLAA
                     LVLRRSTALVGLVFIGALFVLYVVVAHMLFVEARVWLNMVYPLMALVATYTMLTVYRY
                     VLEERERRRIKQAFQYYVAPDIVDLMLQDPGGIRLGGREEVLTALFCDLEGFTNFSEK
                     QAPSEIIGVIGDYYAEMTERVFAEQGTLVEYVGDELFALFGAPISQPDHARRACATAL
                     AMRERRNSLSEEWVKIGRPRLRARTGVNTGTMLVGNIGSKYRFHYGAIGDQVNLTSRL
                     EGLNKTYSTEIMISDDTAQLVPGMFILRELDLVRVKGRARALRIHELLASADASLPEK
                     QKQMLELYAAGLAAYREGRWTNALEIFRQCLVLWPADGPSRLMESRCMMFQAEPPPED
                     WGGTFDHLTK"
BASE COUNT          710 a         1458 c         1427 g          695 t
ORIGIN      
        1 cggtactcga tgtccgcggg cgcggcttcg agcgcatcgg cggcgagttc cttgccgcgc
       61 gcgatggcct tgcggcccgc agcgacgacg gccgatccac cgacgaacgc cgagcgcgag
      121 ccgacgctgc cgacgccgtt cgcgcgatcg gtatcgccct gcacgatgcg gatcttttcc
      181 ggcgggacgc cgagaacttc ctcgatcaat tgcgtgtagg tcgtctcgag cccctgcccc
      241 atcgcctggg ttccgctgaa cacgctcacc acgccttccg cgtcgatttc gatgtccacc
      301 gtctcggtgg gcaacgctcc ggtccattcc aggtagacgg acacggcgcg gccgcgcagc
      361 tttccaagcc gggacgaatc ttcttttctt gccggaaagt cgtcccattt cgcccgatgc
      421 aggacccctt ggagcatgcg cccgaaatcg ccgccgtcgt acgtatcgcc gacatgggtc
      481 ctgtacggca gctcatcggc acgcagcaga ttgcggcggc gcagctccac ccggtcgatc
      541 cccatctcgc gcgccgattt ttcgagcagc cgctcgatca ggtagttgcc ttccggccgg
      601 ccggcgccgc gatacgcgcc ggtcgccatg gtattggtga gcactccctt aacgcggtaa
      661 tcgacgaccg gcacgcgata ggcggaggtg gcgaccttcg cgaccatggc gagcggaatc
      721 atcgctgaag agccgaccgg gaccgcgccg atgttggcaa gcatctccat gcgaagcgcg
      781 agaatgcggc cttcgcggtc gagcgcgaga gcggcgcggt agtgctgatc gcgcgccatg
      841 tgcgcggcga ggaactcctc cgcgcggtcg gcgcgccact tcaccggacg gccgagcttg
      901 cgcgccgcgt agcacacgag cgcgtcctcg ggggtgagcc cggtcttcat gccgaagccg
      961 ccgccgatgt cgccgttaac caggcggaaa ttcgcgggcg gctcgccgaa caccgcgccg
     1021 agcagctcgc gcgccgcggt tggcgtctgg cactgcgtgt aaagggtagt gcgcccgttt
     1081 tcctgtacgc cgatcgcgca gcgtggctcg agcgccatcg cattcaggcg ctggttgtgc
     1141 aattcgatgc gcgtgacgtg cgctgcggcc gagaacgcgc tctcggcagc ttccttgttt
     1201 ccataaacgg cttcggccgc gacattgcca cctgcctgtt cccacagaac cggtgcatca
     1261 gcctcgactg ccttgcgtgg atccacgaca caaggcagct cttcgtactc gaccgtgaca
     1321 agctcggccg cgtcctgggc ttgcacgcgc gtctcggcaa cgactgccgc caccggctga
     1381 ccgacataca acacatttcc ttcggcgagc ggcgtgcgcg gcggcgcggc catcggcgag
     1441 ccgtcggcgc gcttgaagag cggcggaaac gggatgtgct tgaccccgtc ggcgcgcagc
     1501 tcctgcatcg accacgcaac gagcacgcct ttgcttttca gggcttccga aagatcgatg
     1561 ctcgagatgc gggcatgggc gtgcggcgag cgcaccagca ccatccacgc ctgcccggga
     1621 aactcccggt cgtcgctgaa aagcccgcta ccccggagca gccgctcgtc ctcgagccgc
     1681 ttctgcgagc gggaagcgcc gaagcgggcc gacgccggat cgaactgaac ctgcagcgag
     1741 actgcgctca tcgacatcca ggtttggatc tccaatgtaa acaagcccgc agcgcgtgcc
     1801 tactcccagc gcgcagccgg taacggcggc gactgtgaac gtctggctga ctgcaaggcg
     1861 agtgtatcca tcgttggaga aaagccgcag cccgtatgtt cccgagggcg agcgtggccg
     1921 ggatcgcgac cgctcggggg cagatggcgc ccaggtggcc ggccccgggt cttcggtcag
     1981 tgtcacgcgg taggcccgca gcaggcttac tttgtaaggt ggtcgaacgt tccgccccag
     2041 tcctccggtg gaggttcggc ttggaacatc atgcagcggc tttccatgag gcgggacggg
     2101 ccatctgctg gccagagcac gaggcactgg cgaaagattt ccagcgcgtt ggtccagcgt
     2161 ccttcgcggt aggcggccag gccggcagca tacaactcga gcatctgctt ctgcttctcg
     2221 ggcaacgagg cgtccgcgct ggcgagaagc tcgtggatcc gaagcgctcg cgcgcgtccc
     2281 ttgacgcgca caaggtcgag ctcgcgcagg atgaacatgc cggggacgag ctgcgcagtg
     2341 tcatcactga tcatgatctc tgtgctgtag gtcttgttca gcccctccag ccgcgaggtg
     2401 aggttgacct ggtctccgat cgcgccgtag tggaagcggt acttcgagcc gatgttgccg
     2461 accagcatgg tgccggtgtt cacgccggtg cgcgccctga ggcgagggcg cccgatcttg
     2521 acccattcct cgctcaggct gttgcggcgc tcgcgcatgg cgagcgcggt ggcgcaggcg
     2581 cggcgcgcgt ggtcgggctg cgagatcggc gcgccgaaga gcgcgaacag ctcgtcgccg
     2641 acgtattcga ccagcgtgcc ctgctcggcg aaaacccgct cggtcatctc ggcgtagtag
     2701 tcgccaatca cgccgatgat ctcgctcggc gcctgcttct cggagaagtt ggtgaagcct
     2761 tcgagatcgc agaaaagcgc ggtcagcacc tcctcgcggc cgcccaggcg gatgccgccc
     2821 gggtcctgga gcatgaggtc gacgatgtcg ggcgccacgt aatactggaa cgcctgcttg
     2881 atgcgccggc gttcgcgctc ctccaggacg tagcggtaca ccgtgagcat ggtgtaggtc
     2941 gccaccagcg ccatgagcgg atacaccatg ttgagccaaa cgcgtgcctc gacgaagagc
     3001 atgtgcgcta cgacgacgta aagcacaaaa agcgcgccga tgaagacgag cccgacgagc
     3061 gcggtcgagc ggcgcagcac cagcgccgcc agcagcccga gggccacgat ggcaaggagg
     3121 tcgaacacct tcgaccacct cggccgctgc atgaagtccc cggcgaggat gttgtcggcc
     3181 acggtggcat gcacttctgg accggggtac agcggcccga atggcgtagt gcggatgtcc
     3241 ccgattccga tcgcggtcgc gccgacgatc acgatctttt cggcgaacgt gcccggcggc
     3301 agccgccccg ccaggatgtc gccggccgaa taggtcggaa aggtccccgc cgggccgcgg
     3361 tagttgatga ggagctgccc ggcctcgtcg gtcggaacca cgcgctcgcc gatgcgcacg
     3421 ccttcggcgc cgtagcgtcc gccggcgcgc accgcgagcg ccgatttccc gaggtaatgc
     3481 cagacgcaga gcactgcgag cggcggaaag agatcctcgc ctgcctgcac cacgagcggc
     3541 atccagcgca ccaccccgtc gaggtccgcc accacgctga aaaagcccga ggacgcggcg
     3601 gcggaggtga aggaggcgag gctggtctgc ggcgcgtagg ccttgacgaa gggcaaagcg
     3661 cccgcgtcgc ggtcgacgta gaccagcgga tacttcgagc ccgcgatgac gtccacccgc
     3721 cgcgcgacct cggccgggtc gagcttgtag ccgacgccgc tttcgtccat gtggaaaaag
     3781 tagccgagca ccaccggggc ggcggaagcc ttgagcgagc gcgccaggag ctggtcgccc
     3841 tcgccggcga cacggttgcg ccgcacgatc tcgtgcagct tggcgttatt gatgttgagc
     3901 gaattgatcg tgcgctcgag ctcgtcggcg agcgtcgcga gctgcgcgtc gtgctcgggc
     3961 tccgagaaca tcacgtcgaa gcccaccacc ttcgcgtcgt cgcgcgcgag cgcatcgatc
     4021 agcgccgcga tgcgcgcccg cggccacggc cagcgcccct cggactccag gctcttctcg
     4081 tcgatcgccg cgatcaccac cgccggcccc ggcttgatcg gcccgcgcgc gcggaagcgc
     4141 aggtcgagcc agttcagctc gatcgcctcg aacatcgccg gggaaatcgc gaacagcgcc
     4201 acgacgatcg cgatcgttcc cgccgtgatc atggcggggt taagcagcca gcggctcacc
     4261 ccgccaatct actgcgccgc cgcctgcggc
//
LOCUS       DASUNT010000313         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1094154, whole genome shotgun sequence.
ACCESSION   DASUNT010000313 DASUNT010000000
VERSION     DASUNT010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094154"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFX94_06315"
     CDS             <1..483
                     /locus_tag="VFX94_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5767831.1"
                     /translation="IGEVMDDLLEADATPCSLCARLRRGVLYRIAGEVGATKIALGHH
                     RDDILATFFLNLFHGGQLKAMPAKLRSDDDRQVVIRPLAYVAEDDLAAYAEAKGFPII
                     PCTLCGSQDNLERKQIGEMLRDWKRRQPGRIESIARALTEVRPSHLLDRKLYDFLNFR
                     "
     gene            533..1276
                     /locus_tag="VFX94_06320"
     CDS             533..1276
                     /locus_tag="VFX94_06320"
                     /inference="COORDINATES: protein motif:HMM:NF023984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HEX5767832.1"
                     /translation="MTRPVARFIGRSSLIGLLAFIGASVTAEDYDSLALDPAQASEPV
                     VQVWGARTRGAKGIFGVHTWVAVKPEGAAEFTVYEVVGWRLRWSESALVIRNRAPDSW
                     FGAQGELYAEKRGPGVEELIRRIDKAAHEYPFAKTYSLWPGPNSNTFTAWIARAVPEL
                     ETDLPATAIGKDYIGSDLFASAPSGSGFQFSLRGLFGVAASGVDGFELNVLGLNFGVS
                     PSGIKLPLLGRIGAARIQPAVPAEQPALR"
     gene            complement(1287..>2267)
                     /locus_tag="VFX94_06325"
     CDS             complement(1287..>2267)
                     /locus_tag="VFX94_06325"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5767833.1"
                     /translation="AIFLGGMVLLGIQPGIMLVETQLDLVYTIIWTLAIANVVGAGLC
                     FVASPFVARITTLPYAYVAPFMLMVIFFAAYQATSHWGDILALLAIGVLGLYMRRFGW
                     PRPPLIVGFVLAQGAENYLYQAIQFHGWAWVLRPGVLIIAAITAASMWAGARYGRAQI
                     DEGGASARVRDIRPQLAFAIVMTALFAYAVLDLQRWSFLAKAFPLGVAVAALPVALFV
                     AAVICRRSHASAVIFDTEAETAPGVTALGRYLLWIAALLAASGLAGFVIGLALFFAAF
                     LHLEARAPIARNALLTASAVLFLTAMSHIFVLDFPRGLLQELVEMPWPLR"
BASE COUNT          415 a          744 c          754 g          354 t
ORIGIN      
        1 atcggggaag tgatggacga tctgctcgaa gcggacgcga cgccgtgctc actctgcgcg
       61 cggctccggc gcggcgtgct ctatcggatc gccggcgaag tgggcgcgac gaagattgcg
      121 ctcggccatc accgtgacga catcctggcg actttcttcc tcaatctgtt ccacggcggg
      181 cagctgaaag cgatgcccgc caagctgcgc tcggacgacg accggcaggt ggtgatccgg
      241 ccgctcgcgt acgtcgccga agacgatctg gcggcctatg ccgaagccaa gggcttcccg
      301 atcattccct gcacattgtg cggctcgcag gataacctcg agcgcaaaca gatcggcgag
      361 atgctccggg actggaagcg ccgccagccg gggcgcatcg agtcgatcgc ccgcgcgctg
      421 accgaggtgc gtccttcgca cctcctcgac cggaagctct acgatttctt gaatttcagg
      481 taactcgtgt gtcatccggt ggggcctaca atgcgtttca atagcgaaga acatgacgcg
      541 ccctgtcgct aggtttatcg gccgctcgag cctcatcggc ttgctggctt tcattggcgc
      601 ctcggtcacc gccgaggact acgacagcct tgcgctggat cccgcccagg caagcgagcc
      661 ggtcgtgcag gtctggggtg cgcgcacgcg aggcgccaaa ggcatcttcg gcgtgcacac
      721 ctgggtagcg gtcaagcccg agggggccgc cgagttcacg gtctacgagg tggtcggctg
      781 gcgcctgcgc tggtccgaga gtgcgctcgt gatccgcaat cgcgcacccg actcctggtt
      841 cggagcgcaa ggcgagcttt atgccgagaa gcgcggcccg ggggtcgagg agctcatccg
      901 ccgcatcgat aaggctgcgc acgagtaccc cttcgccaag acctacagcc tctggccggg
      961 gcctaactcg aacacattca ctgcatggat cgcgcgcgcc gtgcccgagc tcgaaaccga
     1021 cttgccggcg accgcgatcg gcaaggacta catcggctcg gatctattcg ccagcgcgcc
     1081 cagcggcagc ggcttccagt tctcgctgcg tgggcttttc ggcgttgcgg cgagcggggt
     1141 ggacggcttc gagctgaacg ttctagggct caactttggc gtcagtccct ccggcatcaa
     1201 gcttccgctc ctcggacgca tcggtgccgc gcgcatccag cccgccgttc cggccgagca
     1261 gccggcgctg cggtagcgct ccctttctac cttaacggcc acggcatctc gactagttcc
     1321 tgcagcagcc cgcgcgggaa gtcgagcaca aagatgtgcg acatggcggt gagaaacagc
     1381 acggccgaag ccgtgagcag agcgtttcgc gcgatcggcg cacgcgcctc gaggtgtagg
     1441 aaggccgcga agaagagcgc gagcccgatc acgaagccgg cgaggccgct cgcggcgagc
     1501 agcgcggcga tccaaagcaa gtaacggcct agcgcggtca cacccggagc ggtttcagcc
     1561 tcggtgtcga agatcaccgc gctcgcgtgc gatcggcgac agatgacggc cgcgacaaaa
     1621 agcgcaaccg gaagcgccgc gactgcgacg ccgagcggga acgcctttgc caggaacgac
     1681 cagcgctgca ggtcgagcac tgcatacgcg aatagcgccg tcatgacgat cgcgaacgcg
     1741 agctgcggcc ggatatcgcg tacccgagcg ctcgcgccgc cttcgtcgat ctgggctcgg
     1801 ccatagcgtg cgccggccca catcgacgcg gcagtgatcg ccgcaatgat cagcaccccg
     1861 gggcgcagca cccacgccca tccgtggaac tggatcgcct ggtacaggta gttctcggcg
     1921 ccctgagcga gtacgaagcc tacgatgagc ggcgggcgcg gccagccgaa tcgccgcatg
     1981 tagaggccga gcacgccgat cgcgagcagt gcgaggatgt caccccagtg gctagtcgcc
     2041 tggtacgccg cgaagaagat caccatcagc atgaacggcg cgacgtaggc gtacggcagc
     2101 gtcgtaatgc gcgccacgaa gggcgaggcg acgaagcaca ggccggcgcc gaccacgttg
     2161 gcgatcgcca gcgtccagat gatggtgtag acgaggtcga gctgcgtctc gaccagcatg
     2221 attcccggct ggatgccgag cagcaccatg ccgccgagga agatcgc
//
LOCUS       DASUNT010000314         2748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_393548, whole genome shotgun sequence.
ACCESSION   DASUNT010000314 DASUNT010000000
VERSION     DASUNT010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393548"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VFX94_06330"
     CDS             <1..493
                     /locus_tag="VFX94_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767834.1"
                     /translation="ARLLGRLQHGLTPWRRRAGGALRFRAVERDWWRESWRAREQSIE
                     DLESALRADGVPTLRGLDFDAWDLAARCGTLGGARALLAIEEHGGGKQRVRWRLWAHA
                     STAGVAAAAIFTALALGAALDQAWLAATALAAISAALAWRTAIDCGAALGALDRALRR
                     IEP"
     gene            503..2245
                     /locus_tag="VFX94_06335"
     CDS             503..2245
                     /locus_tag="VFX94_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5767835.1"
                     /translation="MGSKYARVVRYALGHWPSLLAILSLSLALSALAALQPWPLKILV
                     DYGLGAAGPAQPRYVVLAALAGLALFAATAALDAAVTRAWSALGQRMVYDLAGDLFLR
                     LQRLSLLFHARRHVGDSITRITSDAWCVHAVTDSILISPARHLFALASVGALAWQLDP
                     GLTWLTITVAPLLAVSAWYFGAHLKGFERRKIEAQARLASFLQQLLGAMPLVQAFAAA
                     PRNRKLFGALAADVVKANRAAAAVGSAYSGVNAVSVTLGMALVLYVGGQRVLAHELSL
                     GSLLVFVAYLRTLDAASRGLLATYGKLRASEVSIERVLEIMDATEAVVDAPGARTLSE
                     PRPAHGGHLVFERVSFAYEPGRPVIKEVSLELEPGETVALVGHTGAGKSTLASLVPRF
                     FDPLGGRVLLDGVDLRGIQLASLRAQVAVVLQEPFLLPLTVAENIGYGSPSASRDEII
                     AAAVAANAHEFIRELPHGYDTVLGEQGATLSGGERQRLAIARAFLKRARVLVMDEPTS
                     ALDSHTEKLVVEALQRLVAGSTTLIIAHRLSTVRHASRVAVLEEGRLIETGTHADLLT
                     AGGRYAQFHRLQSL"
     gene            2242..>2748
                     /locus_tag="VFX94_06340"
     CDS             2242..>2748
                     /locus_tag="VFX94_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX5767836.1"
                     /translation="MKRSWPRLGSYIRSQGRGLAALLGLVLLGVALEALVPWPLKLIV
                     DHVLTGAPLPGLVGWMELLPAAATSAGLLAWLAFAFFLILAAGQAVQLAKALIQAQVA
                     GRMQYNLAENVFEHLQALSLTYHGKAKKGDLVRRVNADTQCVPTLVMGVLSLVTSLLT
                     LIVLFAVMW"
BASE COUNT          402 a          925 c          920 g          501 t
ORIGIN      
        1 cgcccgtctc ctcggcaggc tccagcatgg attgacgcca tggcgccgac gcgctggcgg
       61 cgcgctgcgc tttcgcgcgg tggagcgcga ctggtggcgc gaaagctggc gcgcccgaga
      121 gcagtcgatt gaagacttgg agagcgcgct gcgtgcggac ggcgtaccga cgctgcgcgg
      181 ccttgacttc gatgcctggg acctcgcagc acgttgcggc acgctcggtg gcgcacgggc
      241 gctcctcgcg atcgaggagc acggaggggg caagcagcgg gtgcgctggc gtttgtgggc
      301 acatgcctcg acagcgggcg tcgccgcagc tgcgatattc accgcactgg cgctcggcgc
      361 agcgctcgac caggcctggc tggccgccac ggcgctcgcc gccatctccg cagcgctggc
      421 atggcgcacg gcgatcgatt gcggagccgc gctaggcgcc ctggaccgag cattgcggcg
      481 catagagccg tgacccgccg ccgtgggttc caaatacgcg agagtcgttc gctacgcgct
      541 cgggcactgg ccgagcctcc tggcgatcct gagccttagt ctcgcgctct cggcgcttgc
      601 ggcactgcag ccttggccgc tgaagatcct agtcgactac ggcctgggcg cggcggggcc
      661 ggcgcagcct cgatatgtcg ttcttgcggc gctcgcgggc ctcgcgctct tcgccgcgac
      721 tgcggcgctg gatgcggcag tgaccagggc ctggtcggcg ctcggccaac gcatggtcta
      781 tgacctcgcc ggcgaccttt tcctgcggct gcagcgcctt tctttgctgt tccatgcgcg
      841 gcgtcatgtc ggggactcga tcacacgtat taccagcgac gcctggtgcg tccacgcggt
      901 caccgacagc attctcattt cgccggccag gcacctgttc gcgctcgcct ccgtgggcgc
      961 gctcgcctgg cagctcgatc ccggcctcac atggctgacg atcacagtcg cgccgctcct
     1021 cgccgtctcc gcctggtatt tcggcgcaca cctcaagggc ttcgagcggc gcaaaatcga
     1081 agcgcaagcg cgacttgcgt ccttcctgca gcagttgctc ggggcgatgc cgctagtgca
     1141 ggccttcgcc gcggctccgc gtaatcgtaa gctcttcggc gccttggctg ccgacgtcgt
     1201 gaaagccaat cgcgccgccg cggcggtggg cagcgcgtac tcgggcgtca acgcggtttc
     1261 ggtgaccctc ggcatggcgc tcgtcttata tgtgggcgga caacgtgtcc tggcgcacga
     1321 gttgtcgctc ggcagcctgc tggtgttcgt cgcctatctg cgcacgctcg acgcggcgtc
     1381 gcgcggcctg ctcgcgacct acggcaaact ccgcgcctcc gaagtgagca tcgagcgcgt
     1441 gctcgagatc atggacgcga cagaggcggt ggtcgacgct ccgggcgcac gaaccctctc
     1501 agagccgcgt cctgcccacg gcggtcacct cgtcttcgag cgggtgagct tcgcctatga
     1561 gccgggacgc cccgtgatta aggaggtttc gctggagctc gagccgggcg aaactgtagc
     1621 gctggtgggc cacacaggcg cgggcaagag cacgctcgct tctctcgttc cgcgcttttt
     1681 cgacccgctc gggggccggg tgcttctcga cggcgtggat ctgcgcggta tacagctggc
     1741 gagcctgcgt gcgcaagtcg cggtcgtact gcaggaacca tttctcctgc cgctcacggt
     1801 cgctgaaaat atcggctacg gctctcccag cgcgagccgc gacgagatca tcgccgcggc
     1861 ggtggcggca aacgcgcacg agtttatccg cgagctgccg cacggttacg acacggtgct
     1921 cggcgagcaa ggcgcaacgc tttccggcgg cgagcgacag cggctggcga tcgcgcgggc
     1981 tttcctcaag cgcgcccgcg tgctcgtaat ggatgaaccc acttcggccc tggatagcca
     2041 cacggaaaag ctcgtggtcg aggccttgca gcgcctcgta gcaggcagca ccacgctcat
     2101 cattgcgcac cgcctatcca cggtgcgcca cgcaagccgc gtagcggtac tggaagaagg
     2161 gcggcttata gagaccggta cacacgccga tctcctgaca gccggcggcc gctacgcgca
     2221 attccaccgc ttgcagagcc tatgaagcgg tcgtggccac gtcttggttc gtacatcaga
     2281 tcgcaaggcc gcgggctggc cgcacttctc ggcctggtgc tactcggagt cgcgctcgaa
     2341 gcgctggtgc catggcccct gaagctcatc gtggatcacg tactgaccgg ggcgccgctc
     2401 cccgggctcg tgggctggat ggagctcttg cctgctgcag ccacgagcgc cgggttgctt
     2461 gcatggctgg ccttcgcctt tttcttgata cttgcggccg gccaagccgt gcaactggcg
     2521 aaggcgctga tccaggcaca ggttgccggc cgcatgcaat acaacctcgc agaaaacgtc
     2581 ttcgagcacc tgcaagcgct gtctctgacg tatcacggga aggcgaaaaa gggcgacctg
     2641 gtgcgccggg tgaacgccga cacccaatgc gtgcccactc tcgtgatggg ggttctgtcg
     2701 cttgtgacct cgctgctcac cttgatcgtg ctattcgcgg tcatgtgg
//
LOCUS       DASUNT010000315         2839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_656084, whole genome shotgun sequence.
ACCESSION   DASUNT010000315 DASUNT010000000
VERSION     DASUNT010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656084"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFX94_06345"
     CDS             <1..200
                     /locus_tag="VFX94_06345"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEX5767837.1"
                     /translation="DGKLLYATCSVFSQENDAVIDTFVARQRAAGTRAVRVALADGAA
                     PQGLPDAVRDGFYYALIHKQA"
     gene            212..778
                     /locus_tag="VFX94_06350"
     CDS             212..778
                     /locus_tag="VFX94_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEX5767838.1"
                     /translation="MLRIAAGGVVFGGLLMLLSPAAPVHADEIEVREVALRRVDEGLS
                     LDVDFGFELTPRLAEVLANGVPLYFSVEFELTRRRWYWFDETAASRRLSLRLSYHALS
                     RQYRVSTGLLQQSFPSLEEALNVLQRVRNWLVVDRSVRLADADYEAAVRMRLDTAQLP
                     KPFQLSALTGRELHLDSSWKRFIVRGPR"
     gene            775..>2839
                     /locus_tag="VFX94_06355"
     CDS             775..>2839
                     /locus_tag="VFX94_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5767839.1"
                     /translation="MKWLIVVGGAVAAVGLFLLATASADTTLFARHYPLLLGLNAALA
                     ALLAALVGTQLVILARRLRARVFGARLTLRLLARFAALAVVPGLIVYAVSVQFLTRSI
                     ESWFDVKVDAALEGGLSLGQQVIEQMTVELQGKAHAMALELAERSPAQLAAQLERLRG
                     QLAVEEAVVVSGSGRLVASAAQDLAKLVPELPTAHALHQARMTRAYSAVDATVGRPLA
                     LRVVVPMDVPGEVRFLQLRQSVPASFARSAEAVEAAYRDYRELAISREGLQRIYVVTL
                     TFALLMALFIAVAAAATQSGLLAEPLANLAQAMQAVARGDFSRRAPVTSRDELGVLTE
                     SFNSMTRQLEEAQRGIEANRLATEAAKARLESILANMSAGVLVFDHELRLSLSNHGAS
                     AILGVELDAFAAQMREHFAAQGEADWQLELQLKKTGKTLNARGARLPRAIGAGAVVVF
                     DDITRLIQAQRATAWAEVARRLAHEIKNPLTPIRLSAERLEMKLANRLSREDAETLRR
                     SIETIVNQVAALKAMVDDFRDYARLPAPELKPLDLNALVRQVLALYENSSVPIAKHLA
                     DQLPPVWADGAQIRQVIHNLVQNAQDALEQPAARGASPAIEVRTELAGERVRLAVSDN
                     GGGFPEELLARIFEPYVTTKPRGTGLGLAIVKKIVDEHHGAVTIENRPASGASVSVLL
                     PLAKAA"
BASE COUNT          413 a          956 c          979 g          491 t
ORIGIN      
        1 ccgatggtaa attgttgtat gccacctgct ccgtgttttc ccaggagaac gacgcggtga
       61 tagacacctt cgtcgcgcgc cagcgcgccg ccgggactcg cgccgtgcgt gtggcgcttg
      121 ccgatggcgc agcgccgcag gggctgcccg atgccgtgcg cgacgggttc tattacgcgc
      181 tgatccataa gcaagcctga cgcgacgcgc gatgctgcgc atcgccgcag gcggcgtagt
      241 gttcggcggg ctcctgatgc tgctctcccc ggccgcaccg gtgcacgcgg acgaaatcga
      301 agtgcgcgag gtggcgctgc gcagggtgga tgagggcctc tcgctcgacg tcgatttcgg
      361 cttcgagctc acgccgcgcc tcgccgaggt gctggcgaac ggcgtgccgc tctacttcag
      421 cgtcgaattc gagttgacgc ggcggcgctg gtactggttt gacgaaacgg cggcctcccg
      481 ccgcctcagc ctgaggctgt cctatcatgc gctgtcgcgg caataccgcg tgtcaacggg
      541 cctgctgcag cagagctttc cgagcctcga ggaggcgctt aacgtgctcc agcgcgtgcg
      601 caactggctg gtggtggacc gcagcgtccg ccttgccgac gcggactacg aagccgccgt
      661 gcgcatgcgc ttggatacgg cgcagcttcc gaagccgttt cagctctctg cgctgaccgg
      721 gcgcgagctg catctggatt cgtcctggaa acgctttatc gtgcggggtc cgcgatgaag
      781 tggctgatcg tcgtcggcgg ggcggttgcg gcagtgggac tgttcctgct cgctacggcg
      841 agcgccgata ccacgctttt cgcgcgccac tatcccttgc tgctcggact gaacgccgcg
      901 ctcgcggcct tgctggcagc cctcgtgggg acacagctgg tgatccttgc gcgcaggctg
      961 cgcgcccggg tgttcggggc gcgactcacc ctgcgcctgc ttgcgcgctt cgccgcgctc
     1021 gccgtggttc ccgggctgat cgtctatgcc gtttcggtgc aattcctcac ccgttccatc
     1081 gagtcgtggt tcgacgtcaa ggttgacgcc gcgctcgaag ggggcctcag tctcggtcag
     1141 caggtcatcg agcagatgac cgttgagctg caaggcaagg ctcacgctat ggctctcgag
     1201 cttgccgagc gctcgccggc gcagctcgcc gcccagctgg agcgcctgcg cggccagctc
     1261 gcagtcgagg aagccgtcgt ggtttcgggg agcggcaggc tcgttgcaag cgcagcgcag
     1321 gacctggcga agctcgtgcc tgagcttccc accgcgcatg cgttgcacca ggcgcgcatg
     1381 acgcgcgcct acagcgcggt cgatgcgacg gtgggccggc cgctcgccct gcgagtcgtc
     1441 gtgccgatgg acgtgccagg cgaggtgcgc ttcctgcagc tgcgccagag cgtgccggct
     1501 tcgttcgcgc gcagcgccga agccgtggag gcggcttatc gcgattaccg cgagcttgcg
     1561 atctcgcgcg aggggctcca gcgcatctac gtcgtgacgc tgactttcgc cttgctgatg
     1621 gcgctcttca tcgcggttgc cgctgctgcc acgcagtcgg gcctgctcgc cgaaccgctc
     1681 gccaatctcg cgcaggcgat gcaggccgtg gcgcgcggcg atttctcgcg ccgcgcgccg
     1741 gtcaccagcc gcgacgagct cggcgtgctg accgagtctt tcaactcgat gacgcggcag
     1801 ctcgaggagg cgcagcgcgg catcgaggcg aacaggctcg ccaccgaggc cgccaaggcg
     1861 cggctcgaga gcatcctggc aaacatgtct gccggagtgc ttgtgttcga ccacgagctg
     1921 cgcctgtcgc tctccaacca cggcgcctcg gccatcctag gggtggagct ggacgcgttc
     1981 gccgcgcaga tgcgcgagca cttcgccgca cagggcgagg ctgactggca gctcgagctg
     2041 cagctgaaaa agaccggcaa gacgctgaat gcgcgcggcg cgcggctgcc gcgcgctatc
     2101 ggggctggcg cggtcgtggt gttcgacgac attacacgcc tcatccaggc acagcgcgcc
     2161 actgcgtggg ccgaagtggc gcgccgcctc gcgcacgaga tcaagaatcc gcttaccccg
     2221 atccggctct cggccgagcg cctggagatg aaacttgcga accggctgtc gcgcgaggac
     2281 gccgagacgc tgcggcgctc gatcgagact atcgtcaacc aggtggccgc gctgaaggcc
     2341 atggtcgacg acttccgcga ttatgcgcgt ctgccggcgc ccgagctgaa gccgctcgat
     2401 ttgaacgccc tggtgcggca ggtgctcgcc ctgtacgaaa attcgtcggt gccgatcgcc
     2461 aagcacctcg ccgatcagct gcctcccgtc tgggccgacg gcgcgcagat tcgccaggtg
     2521 atccataacc tggtgcagaa cgcgcaagac gcgctcgagc agcccgccgc gcgcggcgcg
     2581 agccccgcaa tagaggtacg caccgagctc gctggcgagc gtgtgcggct cgcggtgtcg
     2641 gacaacggcg gcggctttcc ggaggagctg ctggcgcgca ttttcgagcc ttacgtcacc
     2701 accaagccgc gcggtaccgg cctcggcctc gcgatcgtca agaagatcgt cgacgagcac
     2761 cacggcgcgg ttacgatcga gaaccggccg gcgagcggcg catcggtgag cgtgctgctg
     2821 ccgcttgcca aggcggcct
//
LOCUS       DASUNT010000316         3692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_918822, whole genome shotgun sequence.
ACCESSION   DASUNT010000316 DASUNT010000000
VERSION     DASUNT010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918822"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /locus_tag="VFX94_06360"
     CDS             <1..168
                     /locus_tag="VFX94_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL28 family ribosomal protein"
                     /protein_id="HEX5767840.1"
                     /translation="NKTIRTFEPNLHYRRFWVESQNRWVRLRVSTAGLRRIDKLGVEA
                     VLAEIKARKKG"
     gene            171..326
                     /gene="rpmG"
                     /locus_tag="VFX94_06365"
     CDS             171..326
                     /gene="rpmG"
                     /locus_tag="VFX94_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX5767841.1"
                     /translation="MREKIKLESSAGTGHFYTTTKNKRTTPDKIEMKKYDPKARKHVL
                     YKETKLK"
     gene            complement(357..1535)
                     /locus_tag="VFX94_06370"
     CDS             complement(357..1535)
                     /locus_tag="VFX94_06370"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX5767842.1"
                     /translation="MLFSGLLDLPWWGYVIAALAMTHVTIASVTIYLHRCQSHRALEL
                     HPIAAHFFRFWLWLTTGMVTKEWAAIHRKHHAKVETADDPHSPQIYGINRVLWRGVFL
                     YVKEAYNRETLERYGHGTPDDWLERKVYTPHALSGVLLLLLSNLLIFGVIPGALIWLT
                     QIAWIPFWAAGVINGVGHYFGYRNYDVPDASTNIVPWGILIGGEELHNNHHAFASSAK
                     LSTKWYEFDLGWMYICILESLGLAKVKRLAPTPRFAHPKPAVDLETLHAVISNRYDVL
                     SRYAKSFRRTYTEELQRLRKWSPRDAEVLRSLKRALLRGQAAAGSEGARLAEALTNSR
                     ALATALAMRQELAALWERSSASKEQLLRHLQDGCRRAEASGIAPLVDFSQRLRSYALV
                     "
     gene            complement(1615..2874)
                     /locus_tag="VFX94_06375"
     CDS             complement(1615..2874)
                     /locus_tag="VFX94_06375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX5767843.1"
                     /translation="MRITRTVIAHARSALTGMLRFEAPADQVLSGYFRNHPSFGQAER
                     AFIAEAVFAVLRRKRSLEAAAGSAQPQALLAAALLRVQGHSARALQELFDENLLRRLR
                     QAPSADLPDAIRADLPDWLWERLAREQGAPEALRIAQGLLNPAPLDLRVNLARTDRAS
                     AQAELSADGLHATPTPYSPAGLRLAGRPAINRHRLFTEGLVEVQDEGSQLLAWLLAPR
                     RGEMVADYCAGAGGKTLAAAMLMRGTGRIYAMDVSPRRLAALAPRAARAGVTNIHTLV
                     VGEDGARARRLAGKLDRVLVDAPCSGFGTLRRNPDLKWRHGPEAIAQLAAKQGSILQA
                     AARLVKPGGRLVYATCSILQEENDAVAAHFEREHPEFTALSCAELLAAQRVELNTGER
                     LRLFPHVHGTDGFFAVAFAKSSFAKAA"
     gene            complement(2871..3554)
                     /locus_tag="VFX94_06380"
     CDS             complement(2871..3554)
                     /locus_tag="VFX94_06380"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX5767844.1"
                     /translation="MRSWFSLLSSLPVAIAAAAPGVPPRIELHYELMRNGTAMAEVVE
                     RLEQGNGAYHLTETWKGQGMYRLLGRATRVSKGELAQLGPRPREFTDERSGRDTQRVT
                     FDWSANTITRRYKGETRTEPVAANTQDRLSFLFALAYLAPKGEPVSFPIADARGTSHH
                     TYKPNGRERLATPAGHFETLKVIRRKEGTGDVSEIWLAAELGYLPVRIVLVEDGGTRY
                     EHMVTRVSR"
     gene            complement(3526..>3692)
                     /locus_tag="VFX94_06385"
     CDS             complement(3526..>3692)
                     /locus_tag="VFX94_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX5767845.1"
                     /translation="RPGDDAAALAARVLTQEHVIYPRAVRWFLEGRLSLADGIVRVRG
                     EDAQLVLASE"
BASE COUNT          626 a         1193 c         1272 g          601 t
ORIGIN      
        1 aacaagacca tccgtacgtt cgagcccaac ctccactacc gacgcttctg ggtcgagagc
       61 cagaaccgct gggtgcgcct gcgcgtctcc accgccgggc tgcgccgtat cgataagctc
      121 ggcgtggaag ccgtgctcgc cgagatcaag gccagaaaga agggttgacc atgcgcgaaa
      181 agatcaagct cgagtcctcg gccggcaccg gccacttcta cacgaccacc aagaacaagc
      241 gcaccacgcc ggacaagatc gagatgaaga agtacgatcc caaggcgcgc aagcacgtcc
      301 tctacaagga aaccaagctg aaatagaaaa gcccgcgttt gccgcgggct tttcccttag
      361 accagcgcgt acgagcgcag gcgctgtgag aagtccacga gcggcgcgat gccgctcgcc
      421 tcggcgcgcc ggcacccgtc ctgcagatgg cgcagcagct gctccttgga ggcgctcgag
      481 cgctcccata gcgccgcaag ctcttggcgc atggcgagcg cggtggcaag cgcgcgggag
      541 ttcgtgagcg cctcggcaag tcgcgcgccc tcgctgccgg cggcagcctg gccgcggagc
      601 agcgcgcgct tcagcgaacg cagcacctcg gcgtcgcgcg gcgaccactt gcgcagccgc
      661 tggagctctt ccgtataggt gcggcggaac gattttgcgt agcgcgagag cacgtcgtag
      721 cggttggaga tcactgcgtg cagcgtctcc aggtcgacgg ccggctttgg gtgcgcaaag
      781 cgcggggtgg gtgccaggcg cttgaccttc gccaggccga gcgattcgag gatgcagatg
      841 tacatccagc cgaggtcgaa ctcataccat ttggtggaga gctttgccga cgaggcgaac
      901 gcgtggtggt tgttgtgcaa ctcctcgccg ccgatcagga tgccccaggg gacgatgttg
      961 gtgctcgcat cgggcacgtc gtagttgcga tagccgaagt agtggccgac cccgttgatg
     1021 acgcccgcgg cccagaaggg aatccaggcg atctgggtca gccagatcag cgccccaggg
     1081 atgactccga agatcagcag gttcgacagc agcagcagga gcacgccgga cagcgcgtgc
     1141 ggcgtgtata ccttgcgctc aagccagtcg tcgggcgtgc cgtgtccgta gcgctccagc
     1201 gtctcgcggt tgtacgcctc cttgacatag agaaagacgc cgcgccagag aacccggttg
     1261 atgccgtaga tctgcgggct atgcggatcg tccgccgtct cgaccttcgc gtggtgcttg
     1321 cgatggatgg cggcccattc cttggtgacc atgccggtcg tcagccagag ccagaagcgg
     1381 aagaagtgcg cggcgatagg atgcagctcc agcgcgcggt gcgactggca gcggtggaga
     1441 tagatggtga ccgacgcgat tgtcacgtgg gtcatcgcga gcgcggcgat gacgtacccc
     1501 caccagggga ggtccagcaa gccggaaaag agcatcagtt cgggttgttc ctagttgggt
     1561 ggttgggaag cggcggccat tctacgcgaa tgctgaccct ttagcgccct ctcgctaagc
     1621 cgctttggca aaagagcttt ttgcaaacgc aacagcgaaa aaaccgtcgg tgccatgaac
     1681 gtgcggaaaa agccgcaggc gctccccggt gttgagttcg acgcgttgcg cggcgagcag
     1741 ttccgcacag gacagggcgg tgaattccgg atgctcacgc tcgaagtgcg ctgctaccgc
     1801 gtcattctcc tcctgcaaga tgctgcaggt cgcgtagaca aggcgcccgc cgggtttcac
     1861 cagccgcgcc gcggcttgca gaatgctgcc ctgcttcgct gcgagttgcg caatcgcctc
     1921 ggggccgtgc cgccacttca ggtccgggtt gcgccgcagc gtgccgaagc cgctgcacgg
     1981 cgcgtcgacc agaacgcgat cgagctttcc ggcgagacgc cgggcgcgtg caccgtcttc
     2041 gccaaccacg agcgtatgga tattggttac ccccgcgcgc gccgcgcgcg gggcgagcgc
     2101 cgcgaggcgc ctgggcgaca catccatggc gtagatgcgg ccggtgccgc gcatcagcat
     2161 cgccgcagcg agcgtcttgc cgccggcgcc ggcgcagtaa tcggcgacca tctcgccccg
     2221 ccgcggggcg agcagccagg cgagaagctg gcttccttcg tcctgaacct cgacgagccc
     2281 ttcggtgaag agccggtggc gattgatggc cggcctgccg gcgaggcgca accccgccgg
     2341 cgaatacggc gtcggcgtcg cgtgcaagcc gtcggcggag agctcggcct gtgcgctcgc
     2401 gcggtctgtc cgcgccagat tgacgcgcaa gtcgagcggc gccggattca agaggccttg
     2461 cgcgatgcgc aacgcttcgg gagcgccctg ctcgcgtgcc agccgctccc acagccagtc
     2521 gggaagatcg gcacggatgg catcggggag atcagccgaa ggcgcctgac gcagcctgcg
     2581 cagcaagttt tcgtcgaaga gctcttgtag agcccgcgcg gagtgcccct gtactcgcag
     2641 caatgccgcg gcgagcagcg cttggggctg cgccgacccg gctgcggcct ccagagagcg
     2701 cttgcgtcgc agcaccgcga acaccgcctc ggcgatgaat gcgcgctcgg cctggccgaa
     2761 gctcgggtgg ttgcggaagt agccggacag gacctggtcc gccggtgctt caaagcgaag
     2821 catgccggtc agcgcgctgc gggcatgcgc gatgaccgtg cgtgtgatcc tcaccgcgag
     2881 acgcgtgtca ccatgtgttc gtagcgcgtc ccgccgtcct ccacgagcac gatgcggacc
     2941 ggcaggtacc ccagctcggc ggcgagccag atctccgaga cgtctcccgt gccttccttg
     3001 cggcggatta cctttagcgt ttcgaaatgg ccggccgggg ttgcgagccg ttcgcggccg
     3061 ttcggcttgt aagtatggtg cgaagtgccg cgtgcgtccg cgatcggaaa gctgaccggc
     3121 tcgccctttg gcgcgaggta ggcgagcgcg aagaggaaag agagccggtc ctgcgtgttc
     3181 gccgcaaccg gctcggtccg cgtttccccc ttgtagcgcc gcgtgatcgt attggcgctc
     3241 cagtcgaacg tgacgcgctg cgtgtcgcgg ccgctgcgct cgtcggtgaa ctcgcgcggc
     3301 cggggtccga gctgcgcgag ctcgcctttg ctcacgcgcg tggcgcggcc gaggagccgg
     3361 tacattccct ggcctttcca ggtctcggtg agatggtagg cgccattgcc ttgctccagg
     3421 cgctccacga cctccgccat tgcggttccg ttgcgcataa gttcgtagtg gagctcgatg
     3481 cgcggcggca cgccgggcgc ggcggccgca atcgcgaccg gcaggctact cagaagcgag
     3541 aaccagctgc gcatcttcgc cccggacgcg cacgatgccg tcggcgaggg aaagcctgcc
     3601 ctcgaggaac cagcgtactg cgcgcggata gatgacgtgc tcctgcgtga gcacgcgagc
     3661 ggcgagcgcg gccgcgtcgt cgccggggcg ca
//
LOCUS       DASUNT010000317         6771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_831933, whole genome shotgun sequence.
ACCESSION   DASUNT010000317 DASUNT010000000
VERSION     DASUNT010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831933"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /gene="ppx"
                     /locus_tag="VFX94_06390"
     CDS             complement(<1..940)
                     /gene="ppx"
                     /locus_tag="VFX94_06390"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEX5767846.1"
                     /translation="MARHDTLAAVDLGSNSFHLEIGRVVDGQIYPLDAVRELVRLGAG
                     LTAEKRIDRATQAAALEALAKFGERLRGFPKAAVRAVGTNALRVAKNSPQFLREARAT
                     LGFPIEVISGREEARLIYLGVAHSLPASVQRRLVVDIGGGSTEFIIGTGLEPLLTESL
                     YMGCVSYSLKYFPEGKIDKSRMRAAELAASQELATLVRGYRDTGWDEAVGSSGTARSI
                     ENILRANDFAEEGLTREGLQRLKNLLLKEGEADPDRIAGLRPTRAPVLPGGLAIMIAA
                     CDALALESIKVSDGALRHGVLYDLLGRVQHRDMREAT"
     gene            1383..3563
                     /gene="ppk1"
                     /locus_tag="VFX94_06395"
     CDS             1383..3563
                     /gene="ppk1"
                     /locus_tag="VFX94_06395"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX5767847.1"
                     /translation="MESEGLEKIPERIAPPAGADITIPAPPSLSGKPAHPVNRRTSLP
                     AQYLNRELGILAFNRRVLAQAADDTVPLLERLRFITIVSSNLDEFFEIRVAGLKEQIK
                     LGLPEPGPDGRAPADVFEAVSREAHALVADQYLLLNQTLFAALAGQRIVFPKREEWNT
                     AQRNWIREYFFRELLPVLTPIGLDPAHPFPRVFNKSLNFAVELEGRDAFGRNSGAAIV
                     QAPRALPRLIRLPQAIAGTAEYAFVFLSSILHAHMDELFSGMKMQGCYQFRVTRNSDL
                     FVEEEEIKNLHTALQGELFHRHFGDAVRLEVADNCSQRMVDFLLQQFGLREADLYRVD
                     GPVNLYRLREVPDQVERPELKYPPFQAGLPPALDSGDIFEVLKKQDILLHHPFQSFLP
                     VIDFIRTTANDPNVIAIKQTVYRTGTDSELMEILVEAARKGKEVTVVVELMARFDEET
                     NLNWAARLEEVGAHVVYGVVGHKTHAKMALVVRREEGGLRRYVHLATGNYHPRTAKLY
                     TDFGLMTANEEMCADVNEVFKQLTGLGRAGKMRHLWQSPFTLHKRIVTAIRNEAAQAA
                     EGKPARIVAKMNALLEPQIIEELYAASVAGVKIELLVRGVCALKPGIEGLSQNIRVRS
                     VVGRFLEHSRIFYFHNGGAEDVYLASADWMDRNFFRRIELCFPVLDAALKRRVIREGL
                     QPYLDDNCQAWVMNADGDYERLKPRRGRRRSAQEELLFTLGIPT"
     gene            3599..3928
                     /locus_tag="VFX94_06400"
     CDS             3599..3928
                     /locus_tag="VFX94_06400"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEX5767848.1"
                     /translation="MLNATHYSLSGLAAAARHEDAFRQELILVALLTPVALWLGDTGV
                     ERALMIGSLILVLIVELLNSAVEATVDRISFESHRLAKRAKDIGSAAVMLSLANVGVV
                     WLLIILS"
     gene            3975..5102
                     /locus_tag="VFX94_06405"
     CDS             3975..5102
                     /locus_tag="VFX94_06405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX5767849.1"
                     /translation="MRIVLVTETYPPEVNGVARTIGLMAEGLQRRGHFVELVRPRQNG
                     RDHAVHNGSFREILVGGIPIPRYTQLKVGLPSRRTLEHRWKEERPDMVHVATEGPLGW
                     SALAAARQLGIRVATDFHTNFHAYTRDYGLGWLARPVAAYLRSFHNRAECTMVPTRQI
                     ADELATQRFERLRVVGRGVDADVFAPARRSTELRASWGADEGTLVALCVSRFAHEKNF
                     ALVIQAYEAMRRIRPDARLVLVGEGPLKKRLQQQNVGYVIAGRKVDGELARHYASADV
                     FLFPSMSETFGNVTLEAMASGLGIVAYDYAAAREVLRHGESALLAPFGEAPTFIAHAE
                     RFAREPELAPRLGKNARVAAAVCTWDRIVADFEAVLLDVAS"
     gene            5089..5622
                     /locus_tag="VFX94_06410"
     CDS             5089..5622
                     /locus_tag="VFX94_06410"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5767850.1"
                     /translation="MWRLDATLARLQYWDERVCVRINRGTRQRPVVLLLQAVSWLGNG
                     IFWYALMLALLVADPSGAAMPLLHMAFVGAVCTCCYKMIKRTTVRVRPYEVNPLVLAG
                     AAVLDRFSFPSGHTLHALAFSLVACAYYPALEVLLWPFTLLTALSRVALGLHYPSDVM
                     AGGVLGACIAGASFFVT"
     gene            complement(5550..6356)
                     /locus_tag="VFX94_06415"
     CDS             complement(5550..6356)
                     /locus_tag="VFX94_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HEX5767851.1"
                     /translation="MRARFFFSALAGTLFAPDTFAWGLQTHVFLAQWALAAVPLADPQ
                     VRAAVARLPRLVLAGACLPDLALAGRMLGLTAFRRAHQWSTLRRLAAACWDEERAIAV
                     GYASHLLADVVAHNRFVPEHERRILNVPHVTHALCEWAMDEHLREGLTSSPADLLGAD
                     CGTLVQATTRTFRCREGVARRAILFLARADKTLRASRLPALCRGALRLVSGESHFGGY
                     VSDAKSFVRQIEAVLRGAEPRLQPEPDQVTKKEAPAMHAPSTPPAITSLG"
     gene            complement(6385..>6771)
                     /locus_tag="VFX94_06420"
     CDS             complement(6385..>6771)
                     /locus_tag="VFX94_06420"
                     /inference="COORDINATES: protein motif:HMM:NF014168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5767852.1"
                     /translation="LLIGRVLLGLLFLVAGIRKIMFYSASVAYFTKLGFPAPEVIGVL
                     AILIELGAGALLVLGWQTRRVAWLLLLFVIIATAMAHRFWEFDGAQYGNQLNHFLKNL
                     AIIGALLYVAALGAGKLSIDERSVTS"
BASE COUNT         1165 a         2282 c         2205 g         1119 t
ORIGIN      
        1 ccgtcgcctc gcgcatatcc cggtgctgca cacggccgag caggtcgtag agcacgccgt
       61 gccgcagggc gccatccgag accttgatcg attccagcgc gagggcgtcg caggcggcga
      121 tcatgatcgc caaaccgccg ggaagcacgg gcgcgcgggt cggccgcaac cccgcgatgc
      181 ggtccggatc ggcttcacct tccttcaaca ggagattttt cagccgctgg agcccttcgc
      241 gcgtcagccc ctcctcggcg aagtcgttcg cgcgcaggat attctcgatc gagcgcgcag
      301 tgcctgacga accaacggcc tcgtcccagc ccgtgtcgcg gtatcccctc acgagcgtgg
      361 cgagctcctg gctcgcggcg agctcggccg ccctcatgcg cgacttgtcg atcttgccct
      421 cgggaaagta cttcaggctg tagctcacgc accccatata aagcgactcg gtgagcagcg
      481 gctccaagcc cgtgccgatg atgaactccg tcgagccgcc gccgatgtcg accaccagcc
      541 ggcgttgcac cgaagccgga agcgaatgcg cgacaccgag gtaaatgagg cgcgcttcct
      601 cgcgcccgga gatcacctcg atcgggaagc cgagcgtggc gcgcgcctcg cgcagaaact
      661 gcggcgagtt cttcgccacg cgcagcgcgt tggtgccgac tgcgcgcacg gccgctttgg
      721 gaaagccgcg cagccgctcg ccaaacttgg cgagcgcctc caacgccgcg gcctgggtgg
      781 cgcggtcgat gcgcttctca gcggtcagtc cggcgccgag ccgcaccagc tcccgcaccg
      841 cgtcgagcgg atagatctgc ccgtcgacca cgcggccgat ctcgaggtgg aagctgttcg
      901 acccgaggtc caccgcggca agcgtgtcgt ggcgcgccat gccgcgcaaa ataacagaac
      961 ggacctagcc ccgcagggcc tgctcgattt ccgcgccctc gcgtgcacat ccttgcccat
     1021 cgtgaagtac gccacgtgct cactgatgtt cttggcgtgg tcgcgatgca tcggccgctc
     1081 tcgacgacgc gcgcgagtac ggagcggccc gaggtcgaaa tgctgcgcgc gtagagcgca
     1141 atcttcttgg cctcctcgcc cacgcgctcg aggtgggtgg tggcgcccag aagcgacacc
     1201 accgtgagcc ggtctgcagg ctttccatcg cgtcggcgag ctggcgcggg aagtgtggtt
     1261 tgcgctcatg gagttacgtt agcggcggtg catgtccgcc atatttcgac ggccgcagta
     1321 acaaaacagt cataaaaaaa gccgccgcgg ctgtgcagaa ttgcgctcag ggggcggttg
     1381 gcatggaatc cgaaggactg gaaaagatcc cggagcgcat cgctcctccg gcaggtgcgg
     1441 acattacaat cccggctccc ccttccctgt ctgggaagcc ggcgcacccg gtgaaccgtc
     1501 gaaccagcct ccctgcgcag tacctcaacc gcgagctcgg catcctggcg ttcaaccgcc
     1561 gggtgctggc gcaggcggcc gacgataccg tgccgctgct cgagcggctg cgcttcatca
     1621 ccatcgtgtc gtcgaacctg gacgagttct tcgagatccg cgtagcgggg ctgaaggagc
     1681 agatcaagct tgggctgccc gagcccggtc cggacggtcg tgcccccgcc gatgtcttcg
     1741 aggcggtaag ccgcgaagcg cacgcgctcg tggccgatca atacctgctg ctcaaccaga
     1801 cgttgtttgc cgcgctcgcc gggcagcgca tcgttttccc gaagcgcgaa gagtggaaca
     1861 ctgcccagcg caactggatc cgcgaatact tcttccgcga gctgcttccg gtcctcaccc
     1921 ccatcggcct cgacccggcg cacccgtttc cgcgcgtgtt caacaagagc ctgaacttcg
     1981 ccgtggagct cgagggacgc gacgcgttcg ggcgcaactc cggcgccgcg atcgtgcagg
     2041 cgccgcgcgc gctgccgcgc ctgatccgcc tgccgcaggc gatcgccggg actgcggaat
     2101 acgcgttcgt gttcctgtcc tcgatcctgc acgcgcacat ggacgagctt ttctccggca
     2161 tgaagatgca ggggtgttac cagtttcgcg tgacgcgcaa ctccgacctc ttcgtcgagg
     2221 aagaggagat caagaacctg cataccgcgc tgcagggaga gctgttccac cgccatttcg
     2281 gcgacgcggt gcggctcgag gtggccgaca actgctcgca gcgcatggtg gatttcctgc
     2341 tgcagcaatt cggcctgcgc gaagcggatc tctaccgcgt ggacggaccg gtcaacctct
     2401 accggctgcg cgaagtgccc gaccaggtcg agcgccccga gctgaaatac ccgccgttcc
     2461 aggccggcct gccgccggcg ctcgacagcg gagatatttt cgaagtgctg aagaagcagg
     2521 acattctgct gcaccacccc ttccagtcgt tcctgccggt gatcgacttc atccgcacca
     2581 ccgccaacga cccgaacgtc atcgcgatca agcagacggt gtaccgcacc ggtacggatt
     2641 cggagctgat ggagatcctg gtcgaagcgg cgcgcaaggg gaaggaagtc accgtggtgg
     2701 tcgaactcat ggcgcgcttc gacgaagaga ccaacctcaa ttgggctgcg cgcctcgaag
     2761 aagtcggcgc gcatgtggtg tacggggtag tcggccacaa gacgcacgcg aagatggcgc
     2821 tcgtggtgcg gcgcgaggaa ggcgggttgc ggcgttacgt gcacctcgcg actggcaact
     2881 atcacccgcg caccgcgaag ctttacaccg atttcggcct catgacggcg aacgaggaaa
     2941 tgtgcgccga cgtaaacgag gtgttcaagc agctcacggg cctcggccgc gccggcaaga
     3001 tgaggcatct gtggcaatcc ccgttcaccc tgcacaagcg tatcgtcacc gcgatccgca
     3061 atgaggcggc gcaggccgcc gagggcaagc cggcgcgcat cgtcgccaag atgaatgcgc
     3121 tactcgagcc gcagatcatc gaggagctct acgccgcctc ggtcgcgggc gtgaagatcg
     3181 agctactggt gcgcggcgtg tgcgcgctca agccgggcat cgaagggctt tcccagaaca
     3241 tccgcgtgcg ctccgtggtc gggcgcttcc tcgagcactc gcgcatcttc tacttccata
     3301 acggcggcgc cgaggacgtg tacctcgcga gcgccgactg gatggaccgc aatttcttcc
     3361 gccgcatcga gctgtgcttc ccggtgctcg acgcggcgct caagcgtcgc gtgatccgcg
     3421 aggggttgca gccctatctc gacgacaatt gccaggcctg ggtgatgaat gccgacggcg
     3481 actacgaaag gctcaagccg cgccgcggcc ggcgacgctc ggcacaggaa gaactgctat
     3541 tcacgctcgg gataccgacc tagaaagccc gcacaagggc aagactggcc tgcgccgcgt
     3601 gctgaacgcc acgcattact cgctctccgg ccttgccgcc gcggcccggc acgaagatgc
     3661 gtttcgccag gagctgatcc tggtcgctct gctcacccct gtggcgcttt ggctgggcga
     3721 cacgggcgtc gagcgcgccc tgatgatcgg cagcctgatc ctcgtcctca tcgtggagct
     3781 gctcaactcg gcggtggaag cgaccgtgga ccgcatctcg ttcgaaagcc accggctggc
     3841 gaagcgcgcg aaggacatcg gcagcgctgc tgtcatgctc tcgctcgcca acgtcggggt
     3901 cgtctggctt ttaataatcc tttcataaag ccgtagcagt ccggtcacgc agcgcgcgta
     3961 gggtgcccgc gagcatgcgc attgtcctgg tgaccgagac ttatccaccc gaagtgaacg
     4021 gggtagcgcg caccatcggc ctcatggccg aggggctgca gcgccgcggc cacttcgtcg
     4081 agctggtgcg gccgcgccag aacggccgcg accatgccgt gcacaacggc agcttccggg
     4141 agattctcgt cggcggcatc ccgatccccc gctacacgca gctcaaggtg ggcctgccct
     4201 cgcgccgcac gcttgagcac cgctggaaag aagagcgccc ggacatggtg cacgttgcga
     4261 cagaaggccc gctcggctgg tccgcgctcg cggcggcacg ccagctgggt atccgcgtgg
     4321 cgacggactt ccacaccaac tttcacgcct acacgcgcga ttacgggctc ggctggctcg
     4381 cgcggccggt ggcggcttac ctgcgcagct tccacaaccg cgccgagtgc accatggtgc
     4441 ccacgcgcca gatcgccgat gaactggcga cgcagcgctt cgagcgcctg cgcgtcgtcg
     4501 gccgcggggt cgatgcagac gtgttcgcgc cggcgcgacg ctcgaccgag ttgcgtgcga
     4561 gctggggggc cgacgaaggc acgctcgtcg cgttgtgcgt cagtcgcttc gcgcacgaga
     4621 agaacttcgc gctcgtcata caggcctacg aggccatgcg ccgcatccgc cccgatgcac
     4681 gcctcgttct cgtcggggag ggtcctctca agaaacgact ccagcagcag aacgtgggct
     4741 acgtcatcgc cggccgcaag gtggacggcg agctcgccag gcattacgca tccgccgacg
     4801 tgtttctttt cccgagcatg agcgaaacgt tcggcaacgt cacgcttgaa gccatggcga
     4861 gcgggctcgg catcgtcgct tacgactacg ccgcagcgcg cgaggtgctg cgtcacggcg
     4921 agagcgcgct gcttgcaccc ttcggcgagg cgccaacctt catcgcgcat gccgagcgct
     4981 tcgcgcgcga gccggagctc gcgccgcgcc tggggaagaa cgcgcgcgtc gcggccgcag
     5041 tctgcacctg ggaccgcata gtcgccgatt tcgaggcggt gctgcttgat gtggcgtctt
     5101 gatgccacgc tcgcacgcct ccaatactgg gacgagcggg tctgcgtaag aatcaaccgc
     5161 ggcacgcgcc agcgcccggt agtgctctta ctgcaggcgg tcagctggct cggcaacggc
     5221 attttctggt acgcgctcat gctggcgctg ctcgtggccg acccgagcgg ggccgcgatg
     5281 ccgctactgc acatggcttt tgtcggtgcg gtatgcacct gctgctacaa gatgatcaag
     5341 cgcaccaccg tacgcgtgcg cccctacgag gtgaatcccc tcgttttggc cggcgccgcg
     5401 gtgcttgacc gcttcagctt tccctccggc cacacgctgc acgcgctggc gttcagcctg
     5461 gtggcgtgcg cctactaccc cgcgcttgag gtgctcctct ggccgttcac gctgctcacc
     5521 gctctttccc gcgtggcgct cggactgcac tacccgagcg acgtgatggc cggcggcgtg
     5581 ctgggcgcgt gcatcgctgg cgcttctttc ttcgtcacct gatcgggctc gggttgcagc
     5641 cgaggctcgg caccgcgcag cactgcctcg atctgcctga cgaacgactt cgcgtcgctc
     5701 acgtagccgc caaaatgcga ctctccactg acgaggcgca gtgcaccgcg gcagagcgcg
     5761 ggcaggcgcg acgcgcggag cgttttgtcc gcgcgcgcga ggaacaggat ggcgcggcgg
     5821 gcgacgcctt ctcgacagcg gaaagttctc gtcgtcgcct ggaccagcgt accgcaatcc
     5881 gcgccgagta gatcagccgg tgaagaagtc agtccttctc gaaggtgctc atccatcgcc
     5941 cactcgcaca gcgcgtgcgt gacgtgcgga acgttgagga tgcggcgctc gtgctcggga
     6001 acgaagcggt tatgcgccac cacgtcggcg agcaggtggc ttgcatagcc gaccgcgata
     6061 gcgcgctctt cgtcccagca ggcggcagcc agccggcgca gcgtggacca ctggtgcgcg
     6121 cgccggaacg ccgtcagccc gagcatgcgg ccggcaagcg cgaggtcggg aaggcacgcg
     6181 cccgcgagca ccagccgggg cagacgcgcc accgcggcgc ggacctgcgg atcggcgagc
     6241 ggcacggcgg cgagcgccca ttgcgcgagg aatacgtggg tctgcagccc ccaggcgaat
     6301 gtgtcggggg cgaagagggt gccggcaagc gcgctgaaaa agaaacgggc cctcacgggc
     6361 ccgcagcatg gacgagcgtg gttactagct cgttaccgag cgctcatcta tgctgagttt
     6421 tcccgccccc agcgcggcga cgtacaggag tgcaccgata atcgcgagat tcttaaggaa
     6481 gtggttcagt tggtttccgt actgcgcgcc gtcgaactcc cagaagcggt gcgccattgc
     6541 cgtagcgatg atcacaaaaa gcaggagtag ccaggccaca cgccgtgtct gccaacccag
     6601 gacaagcaac gcccccgccc ccagctcgat caggatggca agcacgccta tgacctcggg
     6661 cgccgggaag ccaagcttgg tgaagtatgc gacgctagcg gaataaaaca tgatcttgcg
     6721 gatgccagcg accaggaaca gtaagccgag cagcacgcgt ccgatgagca g
//
LOCUS       DASUNT010000318         2434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1271358, whole genome shotgun sequence.
ACCESSION   DASUNT010000318 DASUNT010000000
VERSION     DASUNT010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271358"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..522
                     /locus_tag="VFX94_06425"
     CDS             46..522
                     /locus_tag="VFX94_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767853.1"
                     /translation="MTRRGLLLTLTEPPPAMEEEFNAWYDTEHLAERLAIPGFRSARR
                     WVRGGIYLATYELDSPEVLQSRPYLERFRNHSPWSRRCLGKCLTFKRWACTQIQPGDA
                     EPHGAALALAFTQEPKSFPALLQSRRFAAESGEAICLYELSDARQQSDDVYRLYGT"
     gene            497..1405
                     /locus_tag="VFX94_06430"
     CDS             497..1405
                     /locus_tag="VFX94_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX5767854.1"
                     /translation="MFTGSTELERYAWLSIAAALATITLKTLAWWLTGSVGLLSDALE
                     SIVNLAAALLALTMLRLAASPPDEKHPYGFSKAEYFSAGIEGALIVLAAAGILASAIP
                     RLIDPEPLTAPVLGLTLSAAASGINLAVGMLLIRVGKTQHSIALEADGHHLMSDVWTS
                     AGVIVGVALVFLTGWLRLDPLVALAVAVHIVWTGLRLMRRSWKGLLDAAISSEDTGEV
                     TKLFAEYSRRYGVSFHALRTRQAGARRFISFHFLVPDEWSVAQAHRLSEEIEERIRSM
                     VPNAAVFVHLEPISDPSSYDDQDLDR"
     gene            complement(1409..>2434)
                     /locus_tag="VFX94_06435"
     CDS             complement(1409..>2434)
                     /locus_tag="VFX94_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5767855.1"
                     /translation="GRPKGVVYHHRGTFVEACGNVMAWAMPANPVYLWTLPMFHCNGW
                     CFPWSVTAMAGTHVCLRAVEPRRIFELIVEHGVTHMCGAPTVLGMLIAAPEEERRKFP
                     HPVHIQTGGSPPPAKVIKAMEELGFQVLHIYGMTELQGPSTLCAPQGEWQDRYAEMAR
                     QGVRYPVVEGHIVAEPRSCKPVPKDGKTMGEILVRGNTVMLGYLKDEKATAEAFEGGW
                     MHTGDLAVWHPSNYVEIKDRKKDIIISGGENISSVEVEIALYKHPATQLAAVVARPDE
                     KWGETPCAFVQLKPGASATEQDYLEWCRQNLARFKIPKKVVFGPVPTTATGKIQKYVL
                     RERAKAL"
BASE COUNT          371 a          860 c          756 g          447 t
ORIGIN      
        1 cgccgatcgc gcctacgtcc tggagaaagg cgtgatcggc cccggatgac gagacgcggc
       61 ctcctcctca cgctgaccga gccgccgccg gcgatggagg aggaattcaa cgcctggtac
      121 gacaccgagc accttgccga gcgcctggcg atccccggtt ttcggtccgc gcggcgctgg
      181 gtgcgcggcg gcatctatct cgcgacctat gaactcgact cgccggaagt ccttcaatcc
      241 aggccttacc tcgaaaggtt tcgtaaccac tcgccctggt cgcgccgctg cctcggcaag
      301 tgcctgacct tcaagcgctg ggcttgcacg caaatccaac ccggcgacgc cgaaccgcat
      361 ggcgcggcgc tcgccctcgc cttcacccag gagccgaaga gcttcccggc gctcctgcag
      421 tcgaggcgct tcgctgccga atcgggcgaa gcgatttgcc tctacgagct ttccgacgcg
      481 cgacagcaaa gcgacgatgt ttaccggctc tacggaactt gagcgctacg cgtggctgtc
      541 gatcgctgcg gcgctcgcca cgatcacgct gaagaccctc gcatggtggc tcaccgggtc
      601 ggtcggcctg ctctccgacg cgctcgaatc gatcgtgaac ctcgcggcgg cgctcctcgc
      661 gcttaccatg cttcgcctgg cggcatcgcc gccggacgaa aaacacccct acggcttctc
      721 gaaagcggaa tacttctctg ccggcatcga aggcgcgctg atcgtgctgg cggccgccgg
      781 aatcctggcg agcgcgattc cgcgtctcat cgaccccgag ccgctcacgg cgccggtgct
      841 tggcctcacc ctcagtgccg ccgcttcggg catcaacctc gcggtcggca tgctgctcat
      901 ccgcgtcggc aagacgcagc acagcatagc gctcgaagcc gacgggcatc acctcatgag
      961 cgacgtgtgg acctcggccg gcgtcatcgt cggcgtggcg ctcgtcttcc tcaccgggtg
     1021 gctgcgcctg gatcccctgg tcgcgctggc ggtggcggtg cacatcgtgt ggacggggct
     1081 acgcctcatg cgccgctcgt ggaagggact cctcgacgcg gcgatctcga gcgaggacac
     1141 gggcgaagtc accaagctct tcgcggagta ctccaggcgc tacggcgtga gcttccacgc
     1201 cttgcgcacg cggcaggcgg gcgcgcgccg cttcatctcc ttccacttcc tcgtgcccga
     1261 tgagtggagc gttgcccagg cgcaccgcct gtcggaggag atcgaggagc gcatccgctc
     1321 gatggtgccg aacgcggccg tgttcgttca cctggagccg atttccgacc cctcgtccta
     1381 cgacgaccag gacctcgatc gctgaagctc agagcgcctt cgcccgctcg cgcaagacgt
     1441 acttctggat cttcccggtc gcggtggtcg gcaccggccc gaacaccacc tttttcggga
     1501 tcttgaagcg cgcaaggttc tgccggcacc actcgaggta gtcctgctcc gtggcgctcg
     1561 ccccgggctt gagctgcacg aaggcgcacg gcgtttcacc ccacttctcg tctggccgcg
     1621 cgaccaccgc ggcgagctgc gtggcgggat gcttgtagag cgcgatctcc acctccaccg
     1681 aggagatgtt ctcgccgccg gaaatgatga tgtctttctt gcggtccttg atctcgacgt
     1741 agttgctcgg gtgccatacc gccaggtcgc cggtgtgcat ccacccgcct tcgaacgcct
     1801 ccgccgtggc tttctcatcc ttgaggtagc cgagcatgac ggtgttaccc ctcaccagga
     1861 tctcgcccat ggttttcccg tccttcggca cgggcttgca gcttctcggc tcggccacga
     1921 tgtgtccttc caccaccggg taacgcactc cttgcctcgc catctcggca tagcgatcct
     1981 gccactcgcc ttgcggcgcg cagagcgtcg acggcccctg gagctcggtc atcccgtaaa
     2041 tgtgaagtac ctggaagcca agctcctcca tcgccttgat gaccttcgcc ggcggcggcg
     2101 agccgccggt ctggatgtgc accgggtgcg ggaacttgcg ccgctcctct tccggcgcgg
     2161 cgatcagcat gccgagcacg gtgggcgctc cgcacatgtg agtgacgccg tgctcgacga
     2221 tgagctcgaa gatgcgcctg ggctcgaccg cgcgcaggca gacatgggtt ccggccatgg
     2281 cggtgaccga ccacgggaag caccagccgt tgcagtggaa catgggtaac gtccagaggt
     2341 acacgggatt cgctggcatg gcccatgcca tcacgttgcc gcaggcctcc acgaacgtgc
     2401 cgcggtggtg atagaccacg cctttcggcc ggcc
//
LOCUS       DASUNT010000319         2714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_42836, whole genome shotgun sequence.
ACCESSION   DASUNT010000319 DASUNT010000000
VERSION     DASUNT010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42836"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VFX94_06440"
     CDS             complement(<1..835)
                     /locus_tag="VFX94_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5767856.1"
                     /translation="MAHRLVLLLLALLLPVCVAAQTLDKVRKTGVLTLGYIDGAAPFS
                     FVDGNGEPQGYSVELCRAVADGVAAQLKRTGLKTRWVKLTIQNRIDAVRKGQVDLECS
                     TTTWTLARQSLVDFSLITFVDGGSLLVKSDAQFGRLADFQGKRIAVITGPTTERALGS
                     RALGAELVKIKTRDEGLALLREGQVDALASDRTALIGVVVMGSGASGNFKLLDQDFSI
                     EQDALMLPRGDHDVRLAVNRQLARIYRSGDIEKIYARWLGPLGPPSILLSATYYIQTI
                     SE"
     gene            complement(820..1185)
                     /locus_tag="VFX94_06445"
     CDS             complement(820..1185)
                     /locus_tag="VFX94_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767857.1"
                     /translation="MPTTVKVIQAKDFVRANPEGHADMAKAEQLLKNIARAGAGLEDF
                     HVLVDTRRSTGALSANELWSLAQQLAQYRHAFGRKTAILCPLERFDHSRFFALCAENR
                     GFDMRAFVSYEEAMEWLID"
     gene            1324..1959
                     /locus_tag="VFX94_06450"
     CDS             1324..1959
                     /locus_tag="VFX94_06450"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX5767858.1"
                     /translation="MAAALLAALPAAAQSGRAGRSEIYIGPVFTDGKNYSFEGGSSVR
                     TDTGFGINFGYAYNFNSHVQAGVDLVWSEADYRATVQPGPGNPNSAATVNSTLETGTV
                     RFFGSYHFLPGQFTPFVTGGLGWTYIDSNIPSGLPDLICWYYPWYGQYCASYVPTYST
                     TRFSYNAGLGLRYDAGRGVFKLLVNSQWADFGGSYGSASVVQYRLDFGTKF"
     gene            1968..2546
                     /locus_tag="VFX94_06455"
     CDS             1968..2546
                     /locus_tag="VFX94_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767859.1"
                     /translation="MKRAVLALLCMSLVPAYAQQVKDAAVAVEETEAMVTVTKVDKEK
                     RTVTFRGPKGGVATLKVPEQSQNLDQVKPGQMYKVKYVEAVAVAIEKGGKPSSSVGEE
                     VKLAPKGAKPGGTVVRTTQLAGVIDSIDYTNRYVAVRGPKGNTVALKAAADVPLEGLS
                     AGDRISVTHTEALAIEMAAQAPAKAKSEAPKK"
BASE COUNT          497 a          879 c          882 g          456 t
ORIGIN      
        1 actcggaaat cgtctggatg taataggtcg ccgagagcag gatgcttggc ggcccgagcg
       61 ggcccagcca gcgcgcgtag atcttttcga tgtcacccga gcggtagatg cgcgcaagct
      121 ggcggttcac tgcaagacgg acatcgtggt cgccgcgcgg cagcatgagc gcgtcctgct
      181 cgatggagaa gtcctggtcg agcagcttga aattgcccga ggcacccgat cccatgacca
      241 ccacgccgat gagtgcggtg cgatccgagg cgagcgcgtc gacctggcct tcgcgcagga
      301 gcgcgagccc ttcgtcgcgc gtcttgatct tcacgagctc tgcaccgagc gcgcggctgc
      361 cgagcgcgcg ctcggtcgtc gggccggtaa tcaccgcgat gcgcttgccc tggaaatcag
      421 cgagccggcc gaattgcgca tcggacttca cgagcaggct gccgccgtcg acgaaggtga
      481 tcaggctgaa gtcgaccagg ctctggcgcg cgagcgtcca cgtggtggtg ctgcactcca
      541 gatccacctg tcccttgcgc accgcgtcga tgcggttctg gatcgtgagc tttacccagc
      601 gggtcttcag gccggtgcgc ttcagctgcg ccgcgacgcc gtcggcgacc gcgcggcaca
      661 gttcgaccga gtagccctgc ggctcgccgt tgccgtcgac gaaggagaag ggcgcggcgc
      721 cgtcgatgta gccgagcgtc agcacgccgg tcttgcgcac cttgtccagc gtttgcgcag
      781 ccacgcatac aggcagcagc agggcgagca gcagcagaac tagtcgatga gccattccat
      841 tgcttcctca taggagacaa aagcacgcat gtcgaatccg cggttctccg cgcaaagggc
      901 gaagaaccgg ctatggtcga agcgctcaag aggacacagg atcgcggtct tgcgcccgaa
      961 ggcgtgccgg tactgcgcaa gttgctgcgc gagcgaccat agctcgttcg cgctgagagc
     1021 gcccgtggag cggcgcgtgt cgacgagcac gtgaaagtcc tcgagccccg cgccagcccg
     1081 ggcgatgttc ttcagcagct gctcggcttt tgccatgtcg gcgtgcccct cggggttggc
     1141 gcgcacgaag tccttggcct gaattacttt gacggtggta ggcacggcat ggcccccagc
     1201 ccaagctaca cgaagccgcg ccgcgctctc actagaccaa ggtccgatag ccgcagccgc
     1261 ggtacggccc aacaatcgct gcaacctcat gaaagaaagc agatgatggc tcgaacgtta
     1321 ttgttggcgg cggcgctgct cgccgcacta ccggccgcgg cgcagagcgg acgtgccggg
     1381 cgcagcgaga tctacatcgg cccggtgttc accgacggca agaactattc gttcgagggc
     1441 ggctcgagcg tgcgcaccga tacggggttc ggcatcaact tcggctacgc ctacaacttc
     1501 aactcccatg tccaggccgg ggtcgacctc gtatggagcg aagcggacta tcgtgcgacc
     1561 gtgcagccgg gaccgggtaa cccgaattcc gccgccacgg tcaacagcac gctcgaaaca
     1621 ggaactgtgc gcttcttcgg ttcgtaccat ttccttccgg ggcaattcac cccctttgtg
     1681 accggaggcc tgggctggac ctatatcgac agcaacattc cgtccggcct gccggacctt
     1741 atttgctggt actacccttg gtacggccag tactgtgcga gctacgtacc gacgtacagc
     1801 actacccgct tcagctacaa cgccggcctg ggcttgcgct acgacgccgg ccgcggcgtc
     1861 ttcaagctcc tcgtcaactc gcagtgggcc gacttcggcg gctcctacgg ctcagccagc
     1921 gtggttcagt accggctcga tttcggaacc aagttctagg agatctcatg aaacgcgcag
     1981 tactcgcatt gctctgcatg agccttgttc cggcttatgc ccaacaggtg aaggacgccg
     2041 cggtcgccgt ggaagagacc gaagcgatgg tgacggtgac caaggtggac aaggaaaagc
     2101 gcaccgtgac ctttcgcggg ccgaaaggcg gcgtcgcgac gctgaaggtt cccgagcagt
     2161 cgcagaacct ggaccaggtt aagcccggcc agatgtacaa ggtgaaatac gtggaagccg
     2221 tggcggtcgc gatcgagaag ggcggcaagc cttcctcctc cgtcggcgaa gaagtcaagc
     2281 tcgcgcccaa gggcgcgaag cccggcggca ccgtggtgcg caccacgcag cttgccggcg
     2341 tgatcgactc gatcgactat accaaccgct acgtcgcggt gcgcgggccg aagggcaata
     2401 cggtcgcgct caaggcggcc gccgacgtgc cgctcgaagg gctttccgcc ggcgatcgca
     2461 tctcggtcac ccataccgag gcactcgcga tcgagatggc ggcacaggca cccgccaagg
     2521 cgaagtccga agcccccaag aagtgacgta gaccagcctc gcatgaagcg gctggcctat
     2581 gccctcgcgg gggtcatagc gctggccgcg gtcctgctcg tcgccttcgg cccggcgctc
     2641 gtcgacacac cggcggtgcg cgcggagatc cagcgccgcc tctcggccgc cgtggacgga
     2701 gaggttgcgt ggga
//
LOCUS       DASUNT010000320         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1095103, whole genome shotgun sequence.
ACCESSION   DASUNT010000320 DASUNT010000000
VERSION     DASUNT010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095103"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="VFX94_06460"
     CDS             complement(<1..1488)
                     /locus_tag="VFX94_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEX5767860.1"
                     /translation="MKAPAHPFRPAAADWSQAIAVAAVLFALYAATSPRTVAGEDDAL
                     FVLSSYYRGIEHPPGYPLFTLIGHVFTYLPFGSVAYRVHLASAFFGALSGAAAWFCAR
                     ALMPGRLPAYLAAFALGLTQVFWSQAIIAEVYTLNAFFFLVLAYLGLKVSPPGPAGAT
                     TAEARRLLPWLALVFGLSLSNHYPLMLLVAPAFVVLLWPARREMLERSGLLLLAVAAG
                     LLPYVWMVRRSWDALPINFDGPLETLPEIWFFVSRAGYAGIDNSPSADMLDRFRFLRF
                     FAGQLIVQFAVAGTALAAAGFIVQARLLGRRVAAFLTIAFLMPSVVLIGLLGFDYSAV
                     YKHMFHVYLLPAYAVGALWIALGMHWLVQRYALRPSHAVAGAAGVAALILAVGARSNA
                     LASHDWSARYAQAVLSALPPQAVVFAQGDPDLAPIAYFMMVENRRPDVMLYQAKGLVL
                     GNRLFHPLRTDEQTQQRLLREFVEEPAGPVVFTLEAFMAYARRDRW"
     gene            complement(1485..2579)
                     /locus_tag="VFX94_06465"
     CDS             complement(1485..2579)
                     /locus_tag="VFX94_06465"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5767861.1"
                     /translation="MPELTVVVPAFRERDNIPRLLRAFEKALEGLDWETIIVCDDGLD
                     GTEQLVRERAQGDHRVRCIQRIGRRGLASACIEGMLASSSPYLAVIDADLQHDESLMP
                     KLLERAKQGGADIVVASRYMEGASTGALAPDRVRISRLASALSRVLAKGLSDPMSGYF
                     LLRRSFLERVMRKLYGRGFKILLDLIAAARGNVSIAELPYSMRSRQHGESKLGARVIA
                     EFFMLILYHLTGRLLPARFFLFAAVGLTGLGLHLAVLTAAFAASGNFLASQLLATWAA
                     MTSNFFLNNVFTYGDQRLRGRALWRGLASFYIACGIGAFINVAVAEWLYWQSVVYWAA
                     GMAGAAVAAIWNFFTTASVTWGPAHNGRRR"
BASE COUNT          526 a          895 c          950 g          348 t
ORIGIN      
        1 ccatcgatcg cgccgcgcgt aggccatgaa ggcctccagg gtgaagacca cagggccggc
       61 cggctcctcc acgaactcgc gcagaaggcg ctgctgcgtc tgctcgtcgg tccgcagagg
      121 atgaaacagc cggttgccga gcacgaggcc tttcgcctga tagagcatga cgtccgggcg
      181 gcggttctcg accatcatga aataggcgat cggcgcgagg tcggggtcgc cctgcgcgaa
      241 caccaccgcc tgcggcggca gcgcgctgag caccgcctgc gcgtagcgag cggaccagtc
      301 gtggctcgcc agcgcattgc tgcgcgcccc gacagccaga atgagcgcgg ccacgccggc
      361 cgcgccggcg acggcatggc tcgggcgtag cgcgtagcgc tgcacgagcc aatgcatgcc
      421 aagcgcgatc caaagcgcac ccacggcgta ggccggaagc aagtacacgt ggaacatgtg
      481 cttgtagacc gcgctgtaat cgaagccgag gaggccgatc agcaccacgg agggcatgag
      541 gaaggcgatg gtaaggaagg cggccacccg ccgccccaga agacgcgcct gaacgatgaa
      601 tccggcggcc gcgagcgccg tgccggccac ggcgaactgc acgatgagct gaccggcgaa
      661 aaagcgcaga aacctgaatc ggtccagcat gtcggcggac ggcgaattgt cgattcccgc
      721 gtatccggcg cggctcacga agaaccatat ctcgggcaaa gtctcgagcg ggccgtcgaa
      781 attgatgggc agcgcgtccc acgaacggcg caccatccag acatacggca gcaagccggc
      841 cgccacggcg agcaggagca gacccgagcg ctcgagcatt tcgcggcgcg ccggccaaag
      901 caacacaaca aacgccggtg cgacgagcag catgaggggg tagtgattgc tgaggctcag
      961 tccgaacacg agcgccaacc acggcagcag gcggcgggct tcggccgtcg tcgcgcccgc
     1021 ggggccgggc ggactcactt tgaggccgag gtacgcaagc accaggaaaa agaacgcgtt
     1081 cagcgtgtag acctcggcga tgatcgcctg cgaccagaac acctgcgtca ggccgagcgc
     1141 gaatgcggcg aggtaggccg gcagccggcc gggcatgagc gcgcgcgcgc agaaccaggc
     1201 ggcggcgccg ctcagagcgc cgaaaaacgc gctggcgagg tggacgcggt aggcgaccga
     1261 gccgaagggg aggtaggtga agacatgccc gatcagcgtg aaaagcggat agccgggcgg
     1321 atgctcgatg ccgcggtagt agctggaaag cacgaacaag gcatcatctt cgccggcgac
     1381 cgtgcgcggc gacgtggcgg catagagcgc gaaaagaacc gcggcgacgg cgatagcttg
     1441 cgaccagtcg gcggcggcgg ggcggaaggg atgcgcgggt gccttcatcg ccgccgcccg
     1501 ttgtgcgcag ggccccaggt gaccgaggct gtggtgaaga agttccatat cgcggccacg
     1561 gcggcgcccg ccatccccgc cgcccagtac acaaccgact gccaatacag ccactccgca
     1621 accgcgacgt tgatgaaggc gccgatgccg caggcgatgt agaagctcgc aaggccgcgc
     1681 cagagcgcgc gcccgcgcag ccgctggtcg ccgtacgtga acacattgtt caggaagaag
     1741 ttgctcgtca tcgcggccca cgtggcgagg agctggctcg caaggaagtt gccgctcgct
     1801 gcgaacgcag ccgtgagcac ggcgaggtgt aacccaaggc cggtaaggcc gacggcggcg
     1861 aagaggaaga agcgcgcggg cagcagcctt ccggtcaggt gatagagaat cagcatgaag
     1921 aattcggcga tcacgcgcgc gccgagcttg ctctcgccgt gctggcggct gcgcatcgaa
     1981 tagggcagct ccgcgatact gacgttgccg cgcgcggccg cgatcaggtc gagcaggatc
     2041 ttgaagcccc gcccgtagag cttgcgcatc acgcgctcga ggaacgagcg tcgcagcagg
     2101 aagtacccgc tcatcggatc gctcaggccc ttggcgagca cccggctgag cgcgctcgcg
     2161 agccggctga tccggacgcg atcgggcgca agcgcgccgg tgcttgcccc ctccatgtaa
     2221 cggctcgcga ccacgatatc cgcgccgccc tgcttcgccc tttcaaggag cttcggcatc
     2281 aggctctcgt cgtgctgcag gtccgcgtcg atcaccgcca gatagggcga ggagctggcg
     2341 agcatgccct cgatgcaggc ggaggcaagg ccgcgccggc cgatgcgctg gatgcagcgc
     2401 acgcggtggt cgccctgcgc gcgctcgcgg accagctgct ccgtgccgtc gaggccgtcg
     2461 tcgcatacga tgatcgtctc ccagtcgagc ccttcgagcg ccttctcgaa cgcgcgcaac
     2521 agccgcggga tgttgtcccg ctcgcggaac gccggcacga ccacggtcag ctccggcacg
     2581 cgtcttctcg ccgccgcgcc cggttcattt cttccacgtg agcacgctga cgaggaagga
     2641 cgcgagcatg agctccatgc cgccgaccat caaggtcgca gccgggatcg tgtcgcgcat
     2701 cgcgacccgc gggtcaagg
//
LOCUS       DASUNT010000321         2231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_42982, whole genome shotgun sequence.
ACCESSION   DASUNT010000321 DASUNT010000000
VERSION     DASUNT010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42982"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFX94_06470"
     CDS             complement(<1..655)
                     /locus_tag="VFX94_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5767862.1"
                     /translation="MSHVSKELEKILEKLEQLLPPPPPEPEWKASVAFRWRSSGRRST
                     PGWLQPVRHVHRIKLSELRGIDKQIERVEQNTRQFLDRKPANNVLLTGARGTGKSSIV
                     KGLLNKYARQGLRLIEVEKNDLVDLPTIVDQVAARPERFILFCDDLSFHGAEEGYLAL
                     KVALDGSISTTSENLLIYATSNRRHLMPEYMAENLETKYLGDEIHPGETVEEKISLSE
                     "
     gene            complement(643..1836)
                     /gene="argJ"
                     /locus_tag="VFX94_06475"
     CDS             complement(643..1836)
                     /gene="argJ"
                     /locus_tag="VFX94_06475"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX5767863.1"
                     /translation="MAVNLAAPEGEALLPVPGIELGVAMAGIKKPGRKDLLVMRLAPG
                     AAVAGVFTKNRFCAAPVLLCRENIHNDIRALVVNTGNANAGTGEEGLKRAREVCEALA
                     GHIGCDTDQVLPFSTGVIMEPLPSERIVAALPKALLAKSDWLSAAEAIMTTDTVPKAS
                     SRRVDFSSASASVTGIAKGAGMIRPDMATMLGFIATDARVSRKDLQRLLERVAERSFN
                     CISVDGDTSTNDSLILAATGLGPEPGRKERVRLEDAVTEVAQRLAQAIVRDGEGATKF
                     VTLRVERGRNRDECRKVAYAVAHSPLVKTAFFASDPNLGRILAAIGNTGVDLSGLELY
                     LDEAVVATGGGRHPEYREEQGVAALKKPEFTVRAVLNRGNADATVWTCDFSYDYVKIN
                     AEYRT"
BASE COUNT          364 a          732 c          710 g          425 t
ORIGIN      
        1 gctcggaaag cgagatcttc tcctccaccg tctcgccggg atggatctcg tcgccgaggt
       61 acttggtctc gaggttctcg gccatgtact cgggcatcag gtggcggcgg ttggaagtcg
      121 cgtagatgag gaggttctcg gaggtggtcg atatcgaccc gtcgagcgcg accttgagcg
      181 cgaggtaccc ttcctcggcg ccgtggaaag agagatcgtc gcagaagagg atgaatcgct
      241 cggggcgcgc ggcgacctgg tcgacgatgg tcggcagatc gacgaggtcg ttcttctcca
      301 cctcgatcag tcgcaggccc tgcctcgcgt acttgttgag cagccctttc acgatggagg
      361 actttcccgt gccgcgcgcg ccggtgagaa gaacgttgtt ggcgggtttg cggtcgagga
      421 actggcgcgt gttctgctcc acccgctcga tctgcttgtc gatgccgcgc agctcggaga
      481 gcttaatccg gtgcacgtgg cgcaccggct gcagccagcc cggcgtgctc cgacgaccgc
      541 tcgagcgcca gcggaacgct acgctcgcct tccattccgg ctcgggaggc ggcggcggca
      601 gcagctgctc cagcttctcc aggatttttt ctagttcttt gctcacgtgc gatattccgc
      661 gttgattttc acgtagtcgt aggagaagtc gcaagtccac acggttgcat cggcgttgcc
      721 gcggttgagg acggcccgta ccgtgaattc cggttttttc agcgccgcaa cgccctgctc
      781 ctcgcggtac tcgggatgac ggccgccgcc cgtggccacc acggcctcgt cgaggtaaag
      841 ctcgagcccc gacaggtcga caccggtgtt gccgatcgca gccaggatgc ggccgaggtt
      901 gggatcggag gcgaagaacg cggtcttcac cagcggcgaa tgcgcaaccg cgtacgccac
      961 cttgcggcac tcgtcgcggt tgcgcccgcg ctcgacgcgc agcgtgacga acttcgttgc
     1021 cccctcgccg tcgcgcacga tcgcctgggc gaggcgctgc gcaacttcgg tgacggcgtc
     1081 ctcgaggcgc acgcgctcct tccttccagg ctcgggtccc aggccggtcg cggcgaggat
     1141 gagcgagtca ttggttgacg tatcgccgtc tacgctgatg cagttgaacg agcgctccgc
     1201 cacgcgctcg agcaggcgct gtaggtcctt gcgcgaaacc cgcgcatccg tagcgatgaa
     1261 gccaagcatc gtagccatgt cagggcgaat cattccggcg cccttggcga tccccgtcac
     1321 gctcgcgctc gccgaggaaa aatccacgcg tctcgaagat gccttgggaa cggtgtcggt
     1381 cgtcatgatc gcctcggcgg ccgagagcca gtcgcttttg gcgagcagcg ccttcggcag
     1441 cgcggccact atgcgctccg agggcagcgg ctccatgatc acaccggtgg aaaacggcag
     1501 cacctgatcg gtatcgcagc cgatatggcc ggcgagcgct tcgcacactt cgcgagcgcg
     1561 cttcaagccc tcctctccgg tgccggcgtt ggcgttaccg gtgttcacca cgagcgcacg
     1621 gatgtcgttg tggatgtttt ccctgcacaa gaggaccgga gcggcacaga agcggttctt
     1681 cgtgaacacc ccggccaccg ccgcgcccgg cgccagccgc atcaccagca ggtccttgcg
     1741 ccccggcttc ttgatcccgg ccatcgccac gccgagctcg atgccgggga cgggaagcaa
     1801 ggcctcccct tcaggggctg cgagatttac cgccacctag gaaagcttgc cctggcagtg
     1861 cttgtactgc ctactcgggg cagtacgcgc ttgttttgaa ggtccttcta gctgcgcgtt
     1921 tagcgccgca caacgactgc cgaacatccg ctgttcccgg ctaggcaatt gacgcgaagc
     1981 cgccgatttt actcgggaat gttcccttgc ccaggatgaa aaggtgatca actagagcaa
     2041 cgaagtcgcc cgcttcactc gatcacccgc gtcgcccgga cgagcaccga accgggcatc
     2101 ttgacaccga gcgcgctggc ggtcttcgcg ttcaccgtca gctcgaactc gtccgcctgc
     2161 tcgacgggaa tgtcgacggg cttcgcacct ttcaggatct ggacgccgat ctccgccgcg
     2221 cggcgagcaa a
//
LOCUS       DASUNT010000322         2737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_394742, whole genome shotgun sequence.
ACCESSION   DASUNT010000322 DASUNT010000000
VERSION     DASUNT010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394742"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VFX94_06480"
     CDS             complement(<1..554)
                     /locus_tag="VFX94_06480"
                     /inference="COORDINATES: protein motif:HMM:NF045284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEX5767864.1"
                     /translation="MEARAKPRDSDLAEEFTRLYYAEVDPEELSARVDADLKGAAVAH
                     LAFARRYSAGTAKARVYNPVRERDGWSSTHTVVEIVNDDMPFLVDSVAMEVNRQGLTL
                     HLLVHPVLRVVRDASGDIRQVGKPGDFSEGRLESFMHVEVDRQTDPAKLAELEAGIAR
                     ALADVRAAVADWKAMQERMREVVRR"
     gene            complement(578..>2737)
                     /gene="putA"
                     /locus_tag="VFX94_06485"
     CDS             complement(578..>2737)
                     /gene="putA"
                     /locus_tag="VFX94_06485"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEX5767865.1"
                     /translation="KRGVGMTLDAEESERLELTLDLHEELTRRFPVGIAVQAYQKRAA
                     AVCEWLASLRRHMSVRLVKGAYWDGEIKRAQQLGMPDYPVFTRKSATDVSYVACAKVL
                     LSAPDYIEPAFATHNCRTVATILHLGGNFEFQKLFGMGDALYDALLAERSIPCRVYAP
                     VGGFTDLLAYLVRRLLENGANTSFVHQIADPDVPVETLVADPLDSLPEPYVPDPRIPL
                     PRDLYPDRRNPLGLDLNDRQTLRDLAARTLADREAGPLAPVEETSPQELEFTVSRAAA
                     AFRSWSQAPALERAALLDNAAARLEERMRELVSLIVREGGRTYNDAVSEVREAADFCR
                     YYALQARQKFDKAIVLSGPAGERNELRLHGRGVFACISPWNFPLAIFTGQVAAALAAG
                     NTVVAKPAEQTPRIGLRALEILYQAGVPPDAACCVVGPGDTTGAALVADPQISGVAFT
                     GSVETARRINEALAKRGGAIVPLIAETGGVNAMIVDASALPEQVVDDTVVSAFQSAGQ
                     RCSAQRILFVDEGCAARVMRLLAGALAELKVGDPAEPDTDIGPIIDAPSRNALLNHIE
                     RLRGEAKLIGETRLPGIPGNFIAPIAFQMPLERLPRVEVFGPVLHVCTYRRDELGRVL
                     QWLNDVGYGLTLGVHSRIQAFADEVVRSARVGNVYVNRSMIGAVVGVQPFGGEGLSGT
                     GPKAGGPHYLLRFATERTLTVNTAAVGGVTELLAGSP"
BASE COUNT          427 a          924 c          944 g          442 t
ORIGIN      
        1 cggcgcacga cttcgcgcat gcgctcctgc atcgctttcc agtcggccac cgccgcgcgc
       61 acatcggcga gcgccctcgc gatacccgcc tcgagctcgg ctagcttcgc cgggtcggtc
      121 tggcggtcca cctcgacatg catgaaggac tcgagccgcc cttcggagaa atcgcccggc
      181 ttgcccactt gcctgatgtc cccacttgcg tcgcgcacca cgcgcagcac cggatgtacg
      241 aggaggtgca gcgtaaggcc ctggcggttg acctccatcg cgaccgagtc gacgagaaac
      301 ggcatgtcgt cgttcacgat ttcgaccacg gtgtgggtgc tcgaccagcc gtcgcgctcg
      361 cgcaccgggt tatagacgcg ggccttcgca gtaccggcgg agtagcgtct tgcgaacgcg
      421 aggtgcgcaa ccgccgcgcc cttcagatcc gcgtccaccc gcgcggaaag ctcttcggga
      481 tcgacctcgg cgtagtaaag ccgagtgaat tcctcggcga gatccgagtc ccgcggcttg
      541 gccctggctt ccatccctgt gcgccgaatt gtaggcccta tgggctgccg gcgagcagtt
      601 cggtcacgcc gccgaccgct gcggtattga cggtcagcgt tctctcggta gcgaacctca
      661 gcaggtaatg cggtccgccg gccttcgggc cggtcccgga cagcccttcg ccgccaaagg
      721 gctgcactcc gaccaccgcg ccgatcatgc tgcggttgac gtagacattg cccactcgtg
      781 ccgagcgcac cacttcgtcc gcgaaggcct ggattcggct gtggacgccc agcgtcaagc
      841 cgtagccgac atcgttgagc cactgcagga cccggccaag ttcgtcccgc ctgtaggtgc
      901 atacgtgaag gaccggcccg aacacttcga ccctgggcag ccgctcgagc ggcatttgga
      961 aggcgatcgg cgcgatgaag ttgcccggga tgccgggcag gcgcgtttcg ccgatcaatt
     1021 tcgcttcccc tctcaacctc tcaatgtgat tcagcagcgc gttgcgcgag ggcgcgtcga
     1081 tgatcgggcc gatgtcggtg tcgggctcgg ccgggtcgcc gaccttgagc tcggcgagcg
     1141 cgccggcgag tagccgcatg acgcgcgccg cgcagccctc gtcgacgaag aggatgcgct
     1201 gcgccgagca gcgctggccg gcgctctgga aggcggacac gacggtgtcg tcgaccacct
     1261 gctcggggag cgccgaggcg tcgacgatca tcgcgttcac gccgccggtc tcggcgatga
     1321 gcggaacgat ggcgccgccg cgctttgcga gcgcttcgtt gatccgcctc gcggtctcga
     1381 ccgagccggt gaaggcgacg ccgctgattt gcgggtcggc caccagcgcc gcacccgtcg
     1441 tatcgccggg ccccacgacg cagcacgccg catcgggcgg aacgccggcc tggtagagga
     1501 tctcgagcgc acgcaggccg atgcgcgggg tctgctccgc cggcttggcg accacggtat
     1561 ttcctgccgc gagggccgcg gccacctggc cggtgaagat cgcgagcggg aaattccagg
     1621 ggctgatgca ggcgaaaacg ccgcgcccgt gcaggcgcag ctcgttgcgc tcgcccgccg
     1681 gcccggagag aacaatggcc ttgtcgaact tctgccgtgc ctgcaacgcg tagtacctgc
     1741 agaaatccgc ggcttcgcgc acttccgaaa ccgcgtcgtt gtaggtgcgg ccgccttcgc
     1801 gcacgataag cgagacgagc tcgcgcatgc gctcctccag tcgcgcggcg gcgttgtcga
     1861 gcagcgcggc ccgctcgagc gcaggggcct gcgaccacga tcggaacgcg gcagcggcgc
     1921 gcgaaaccgt gaactcgagc tcttgcggcg aggtctcttc gaccggcgcg agcggcccgg
     1981 cttcgcgatc ggccaaagtg cgcgctgcca ggtcccgaag cgtttgtcgg tcgttcaaat
     2041 cgaggccaag ggggttgcgg cggtcgggat agagatcgcg cggcagcgga atgcgcggat
     2101 cggggacgta cggctcgggc agcgagtcga gcgggtcggc aacgagcgtt tccaccggga
     2161 cgtcggggtc ggcgatctga tggacgaaag aggtgttggc gccgttctcg agcaggcggc
     2221 gcacgaggta ggccaggaga tcggtgaagc cgccgacggg cgcgtagacg cggcacggga
     2281 tcgagcgctc agcgagaagc gcgtcgtaga gcgcgtcgcc catcccgaag agcttctgga
     2341 actcgaagtt tccaccgagg tgcaggatgg tggccacggt cctgcagttg tgggtggcga
     2401 aagcgggctc gatgtagtcc ggcgcggaga gaagcacctt cgcgcaggct acgtaggaga
     2461 cgtcggtcgc gctcttgcgc gtgaacaccg ggtagtccgg catgccgagc tgctgcgcgc
     2521 gcttgatctc gccgtcccag tacgcgccct tcaccaggcg cacgctcatg tgtcgcctga
     2581 ggctcgcgag ccactcgcac acggcagccg cgcgcttctg gtacgcctgc accgcgatgc
     2641 ccaccgggaa tcgccgcgtc agctcctcat gcaggtcgag cgtgagctcg agccgctccg
     2701 attcctcggc gtcgagcgtc atgccgacgc cccgctt
//
LOCUS       DASUNT010000323         2578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_920363, whole genome shotgun sequence.
ACCESSION   DASUNT010000323 DASUNT010000000
VERSION     DASUNT010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920363"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /locus_tag="VFX94_06490"
     CDS             <1..1511
                     /locus_tag="VFX94_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5767866.1"
                     /translation="WFAAMDKPVGGESLRQTALRGIEDTQFFPSWGKARLHGMIANRP
                     DWTLSRQRQWGVPMPFFLHRETGALHPRTPELLAEVAKRVEREGIEAWQTLRAEELIG
                     NDSKDYEKSRDTLDVWFDSGSTHCTVLRGSHRAESEFPADLYLEGSDQHRGWFHSSLL
                     VSCMLDGVPPYKGLLTHGFVIDLEGRKMSKSKGNTMAPQEISNTLGAEILRLWVASSD
                     YSSELNLSSEILKRVVESYRRVRNTLRFLLANTSDFDLRRDAVAGNDLVEIDRYALAM
                     TVAMQAEVTADYERYQFHLVAQKLLAFCSEDLGAFYLDVLKDRLYTCAADSRPRRSAQ
                     TALWHITHSLVRLMAPILSFTAEELWQVFTKEESVFLTTWHELPAPAGAAELIAKWTR
                     LRALRDPVRKEIEALRAEGKVGSSLQAEVDFGAAGEDYDLLASLGDDLQFLLLTSAAR
                     VRRTGELEVRVKASEHPKCERCWHYRADVNDEALCGRCQSNLRGAGEVRRHV"
     gene            1504..1998
                     /gene="lspA"
                     /locus_tag="VFX94_06495"
     CDS             1504..1998
                     /gene="lspA"
                     /locus_tag="VFX94_06495"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX5767867.1"
                     /translation="MSSAWRWFLLSAAIMAADQLSKWAVLDYFAGREPRQELTGFLNL
                     VLVCNKGAAFSFLANAPGWQTPLFIAFALGAAGLCGALIVRNPGKRLFCAGLALVVGG
                     ALGNVIDRLRFGCVVDYLDFYILDRWPHWPAFNVADSAITIGAVLLILDGFAHHEKRA
                     RAPS"
     gene            2032..>2578
                     /gene="ispH"
                     /locus_tag="VFX94_06500"
     CDS             2032..>2578
                     /gene="ispH"
                     /locus_tag="VFX94_06500"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX5767868.1"
                     /translation="MEVLLANPRGFCAGVERAIETVERALELHGGPIYVRHEIVHNRY
                     VVENLRAKGAVFVEELDEVPPGATVIFSAHGVSKQVRADAERRGLKVFDATCPLVTKV
                     HFEVARMLRAGYEVVMIGHRGHPETEGTMGQAARGMHLIETVDDVASLDIGTPDKIAY
                     VTQTTLSIDDARAIVAALKARF"
BASE COUNT          437 a          814 c          870 g          457 t
ORIGIN      
        1 aatggttcgc ggcgatggat aagcccgtgg gcggtgaaag cctgcggcag accgcgttgc
       61 gcggcatcga ggacacgcag ttcttcccgt cctggggcaa agcgcgcctg cacgggatga
      121 tcgccaaccg gccggactgg acgctgtcgc gccagcggca atggggagtg cccatgcctt
      181 tcttcctcca tcgcgagacc ggcgccctgc atccgcgcac gccggaactg ctggcggagg
      241 tggcgaagcg cgtcgagagg gaaggcatcg aggcctggca aacgctcagg gccgaagaat
      301 tgatcggaaa tgacagcaag gattacgaga agagccgcga cacgctggac gtctggttcg
      361 actcgggctc gacgcactgc accgtgctgc gcggctcgca tcgcgcggag tcggagttcc
      421 cggccgatct atatctcgag ggctcggacc agcaccgcgg ctggttccat tcatcgctgc
      481 tggtgtcctg catgctggac ggtgtgccac cgtacaaggg cctgctgacc cacggcttcg
      541 tcatcgacct cgaaggccgc aagatgtcga agtccaaggg caacacgatg gcgccccagg
      601 agatctccaa cacgctcggg gccgagatcc tgcggctgtg ggtggcaagc agcgattatt
      661 cgagcgagct gaatctctcc agcgagatcc tgaagcgcgt tgtggaaagc taccgccgtg
      721 tgcgcaacac gctacgcttc ctgctcgcca acacctcgga cttcgacctc cgacgcgatg
      781 cagtagccgg caacgatctg gtagagatcg atcgctacgc gcttgccatg acggtggcga
      841 tgcaggcgga ggtgaccgcg gactacgagc gctatcagtt ccacttggtc gcacagaagc
      901 tgcttgcgtt ctgctccgag gacctcggcg ccttctacct cgacgttctc aaggaccgtc
      961 tctatacctg cgccgcggac tcgcggccgc ggcgctcggc gcagaccgcg ctttggcaca
     1021 tcacccacag cctggtgcgg ctgatggcgc cgatcctttc gttcactgcc gaggagctgt
     1081 ggcaggtgtt caccaaggag gaaagcgtgt ttctcaccac ctggcacgag ctccccgcgc
     1141 ctgcgggcgc tgccgagctg atagccaagt ggacccgcct gcgcgcgctg cgcgatccgg
     1201 tacgcaagga gatcgaggcg ctgcgcgcgg agggcaaggt cggttcttcg cttcaggccg
     1261 aggtggactt cggcgccgcg ggcgaggatt acgacctgct cgcaagcctg ggcgacgatc
     1321 tccagtttct gcttcttacg tccgcggccc gcgtccgcag aaccggtgaa ctggaagtga
     1381 gggtgaaggc gagcgagcat ccgaaatgcg aaaggtgctg gcactaccgt gccgatgtga
     1441 acgacgaggc tttgtgcggg cgctgccagt cgaatctgcg cggagccggc gaggttcgcc
     1501 ggcatgtcta gcgcgtggcg ctggttcctg ttgtcggcgg cgatcatggc ggccgaccag
     1561 ctctcgaagt gggcggtgct cgattacttc gccgggcgcg agccgcgcca ggagctcacc
     1621 gggttcttga atctggtgct cgtctgcaac aagggcgcgg cgttcagctt tctcgccaac
     1681 gcccccggat ggcagacgcc gctcttcatc gcattcgcgc tcggcgcggc gggcctgtgt
     1741 ggcgcgctga tcgtgcgcaa ccccgggaag aggttgttct gcgcggggct cgccctggtc
     1801 gtgggcggcg ctctggggaa cgtgatcgac cggctgcgct tcggctgcgt agtggactat
     1861 ctcgacttct acatcctgga tcgctggccg cattggccgg cgttcaacgt ggccgattcc
     1921 gcgattacga tcggcgccgt gctgctcatc ctcgacggct tcgcgcacca tgaaaagcga
     1981 gctcgagctc cgtcttaaat agtcgctgca aacggcatga gaaaatagcc tgtggaagtc
     2041 ctgctcgcga atcccagggg tttttgcgcc ggcgttgagc gcgccattga gacggtagag
     2101 cgcgcgctcg agctgcacgg cgggccgatc tatgtgcgcc acgagatcgt gcacaaccgg
     2161 tacgtggtcg agaacctgcg cgccaagggg gcagtgttcg tcgaggagct cgacgaggtg
     2221 ccccccggcg ccacggtgat tttcagcgcg cacggcgtct cgaagcaggt gcgcgcggac
     2281 gccgagcgcc gcggcctcaa ggtgttcgac gccacctgtc cgctcgtcac caaggtgcac
     2341 ttcgaggtgg cgcgcatgct gcgcgcgggt tacgaggtgg tcatgatcgg ccaccgcgga
     2401 cacccggaga ccgagggcac gatggggcag gcggcgcgcg gcatgcacct catcgagacc
     2461 gtggacgatg tcgcaagcct cgatatcggc acgcccgaca agatcgccta cgtcacacag
     2521 accacgcttt cgatcgacga tgcccgcgcc atcgtggccg cgctcaaggc acgttttc
//
LOCUS       DASUNT010000324         2780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_482881, whole genome shotgun sequence.
ACCESSION   DASUNT010000324 DASUNT010000000
VERSION     DASUNT010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482881"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1320
                     /locus_tag="VFX94_06505"
     CDS             97..1320
                     /locus_tag="VFX94_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5767869.1"
                     /translation="MRQPLAIAALAGALAIAFPAQAKVEGDTIVLGSAISLTGKYSQE
                     GKNAKDGYDLAVKRINETGGVKMGGKTYKLQVKYYDDESTPARTAQLLERLIRQDGVK
                     FVLGPYSSGTTKAAAPITEKHRIPMVEAEGASRSLFTQGYKYLFAVLSTSEQYLKSAI
                     DLAAEVAKQQGKKPSTVRIAMAFENDPFSLDVRAGVVEDAKRYGMDIVIDDKLPRDLS
                     DMTATLTKVKALKPDLLLVSGHTKGATTAARQISEMNVDVPLVAMTHCESADLVGNFG
                     KNAEGILCPTQWAETLSYKGPVFGSAGEYDKLFRKTYSGYETVPYQAAQASAAVIVWK
                     DAFERAKSFDTEKLRDAIAATNLETFYGPIKFGPQGNNIAKPMVLRQIQNGDYNVVAP
                     STWASHKLQYEAAAR"
     gene            1355..2212
                     /locus_tag="VFX94_06510"
     CDS             1355..2212
                     /locus_tag="VFX94_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5767870.1"
                     /translation="MQLVLNGLLIGAVFALAAYGLAIVWGVMNVKNLAQGDFVMLGGY
                     LGYTAAEYGIHPIAALVPVAALMFAFGWVVYVLVIRRVIQRDLFTSLLATFGLAIVLQ
                     QGMNLAFGPEVRTADSGLGTREWFGGIVTVTEIRLLAFALCAALAAALVVFMRRSRIG
                     RAIRATAQDARAARVLGVNVERVYAFTFSLNAAICGAAGVLVAMIWVLQPYYGIAYSV
                     RAFVIVTAAGLGNLPGVISIALGLGVIEQYGGFVLGAQFQQAVVVGLLLLVLMIKQLA
                     LARHRQALQ"
     gene            2226..2471
                     /locus_tag="VFX94_06515"
     CDS             2226..2471
                     /locus_tag="VFX94_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767871.1"
                     /translation="MTQLERTLAKRRALEVIGMALAGDGVLSIVDPVGHTALWAQGPR
                     FWRALVEPFARRPRLTGAVGLGLVVAGVWIAGGAYRR"
     gene            2468..>2780
                     /locus_tag="VFX94_06520"
     CDS             2468..>2780
                     /locus_tag="VFX94_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767872.1"
                     /translation="MTRSHVLQAALLAAALAAPFAFPAHTTQIAVLWIMVLFALTWDL
                     MGGQMGYNSLGNIVFFGVGMYACAIAQIGTFYDVAEYTAAFGAIKVDFSHSQYFGGLP
                     RP"
BASE COUNT          451 a          919 c          913 g          497 t
ORIGIN      
        1 ggaccgccgt tgcgcaattc ggtaggcgga gcgatccgtt aagtcgcgct taagcgccgg
       61 cgggcgaatc tccgcgcata cctctaggag gagcgcatgc gccaaccatt agcaatcgcc
      121 gccctcgcgg gcgccctcgc aattgccttt cccgcacagg cgaaggtcga aggcgacacc
      181 atcgttctcg gctcggcgat ctcactgacc ggcaagtact cgcaggaagg caagaacgcc
      241 aaggacggct acgacctcgc cgtgaagcgc atcaacgaga cgggcggcgt gaagatgggc
      301 ggcaagactt acaagctgca ggtgaagtac tacgacgacg agtcgacgcc agcacgcacc
      361 gcgcagctgc tggaacgcct catccgccag gacggcgtca agttcgtgct cgggccctat
      421 agctcgggca ccacgaaagc cgccgcgccg atcaccgaaa agcaccgcat tccgatggtg
      481 gaggctgagg gcgcatcgcg ctcgctcttc acccagggct acaaatatct cttcgccgtt
      541 ctctccacct ccgagcagta cctgaagagc gcgatcgacc tcgccgccga ggtggcgaag
      601 cagcagggca agaagccctc gactgtgcgc atcgcgatgg cgttcgagaa cgatccgttc
      661 tcgctcgacg tgcgcgccgg ggtggtcgag gacgccaagc gctacggcat ggacatcgtg
      721 atcgacgaca agctgccgcg cgacctctcc gacatgactg ccacgctcac taaggtgaag
      781 gcgctcaagc ccgacctgct gctcgtgtcg ggtcatacca agggcgcgac taccgcggcg
      841 cgccagatct cggagatgaa cgtcgacgtg ccgctggtcg cgatgacgca ctgcgagtcg
      901 gccgacctcg ttggcaactt cgggaagaac gccgaaggga tcctttgccc gacgcaatgg
      961 gccgagacgc tttcctataa agggccggtg ttcggctcgg ccggcgagta cgacaagctc
     1021 ttccgcaaga cctacagcgg gtacgagacc gtgccgtatc aggcggcgca ggcctcggcc
     1081 gcggtgatcg tgtggaagga cgccttcgag cgcgcgaaga gcttcgacac ggagaaactg
     1141 cgcgacgcca ttgccgccac caatctcgag accttctacg gtccgatcaa gttcggaccg
     1201 cagggcaaca acatcgcgaa gcccatggtg ttgcgccaga tccagaacgg cgattacaac
     1261 gtggtggcac cctcgacctg ggcatcgcac aagctgcagt acgaggccgc tgctcgataa
     1321 cctgcagatt ctgacggcgg cgccgctctt caacgtgcag ctcgtgctga acgggctgct
     1381 gatcggagcg gtgttcgcgc tcgccgccta cggtctggcg atcgtctggg gcgtgatgaa
     1441 cgtcaagaac ctcgcccagg gcgacttcgt gatgctgggg ggctacctcg gctacaccgc
     1501 cgctgagtac ggcatccatc cgatcgcggc gctggtcccg gtcgctgcgc tgatgttcgc
     1561 gttcggctgg gtggtgtacg tgcttgtcat ccggcgcgtg atccagcgcg acctgttcac
     1621 ttcgctcctc gccaccttcg ggctcgcgat cgtgctgcag cagggcatga acctcgcgtt
     1681 cggacccgag gtgcgcaccg ccgattccgg ccttggtacg cgcgaatggt tcggcggcat
     1741 cgtcaccgtg accgagatcc gcctccttgc cttcgcgctt tgcgccgcgc tcgcggcggc
     1801 gctggtggtg ttcatgcgga ggagccgcat cgggcgcgcg atccgcgcca ccgcgcagga
     1861 cgcgcgcgcg gcgcgcgtgc tcggcgtgaa cgtcgagcgc gtttacgcct tcaccttttc
     1921 gctcaacgcc gcgatctgcg gcgcggccgg cgtgctggtc gccatgatct gggtgctgca
     1981 accctattac ggcatcgcct actcggtgcg cgccttcgtc atcgtgaccg cggccggcct
     2041 gggcaatctg ccgggggtga tctcgatagc cctcggcctc ggggtgatcg agcagtatgg
     2101 cggcttcgtg ctcggcgcgc aattccagca ggccgtcgtg gtggggctgc tgctcctcgt
     2161 gctgatgatc aagcagctcg ccctcgcgcg ccatcgccag gcgttgcagt gacattggag
     2221 gtgttatgac gcagttggaa aggactctgg ccaagcgccg cgcgctcgaa gtcatcggca
     2281 tggcgctcgc cggcgacggc gtgctgagca tcgtcgaccc cgtgggccac acggccttgt
     2341 gggcgcaagg gccgcgcttc tggcgcgcgc tggtcgagcc gtttgcgcgc cgtcctcggc
     2401 tcaccggcgc ggtcgggctc ggcctggtcg tggcgggcgt gtggatcgcg ggcggcgcct
     2461 accggcgatg acgcgatcgc atgttcttca ggcagcgctg ctcgccgcgg cgctcgccgc
     2521 gccgttcgcg ttcccggccc acactacgca gatcgccgtc ctctggatca tggtgctgtt
     2581 cgcgctcacc tgggatttga tgggcggcca gatgggctac aactcgcttg gcaacatcgt
     2641 cttcttcggc gtgggcatgt acgcttgcgc gatcgcgcag atcggcacgt tctatgacgt
     2701 cgccgaatac accgccgcct ttggcgcgat caaagtggat ttcagccact cgcagtactt
     2761 tggcgggctc ccgcgccccc
//
LOCUS       DASUNT010000325         2910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_920633, whole genome shotgun sequence.
ACCESSION   DASUNT010000325 DASUNT010000000
VERSION     DASUNT010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920633"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..98)
                     /locus_tag="VFX94_06525"
     CDS             complement(<1..98)
                     /locus_tag="VFX94_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX5767873.1"
                     /translation="MQTRIRNFSIIAHIDHGKSTLADRFIQMCGGLA"
     gene            complement(117..317)
                     /locus_tag="VFX94_06530"
     CDS             complement(117..317)
                     /locus_tag="VFX94_06530"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX5767874.1"
                     /translation="MYRRSGCHLCEQMAAELRVRGIAFDEIDVDSDPRLRLKYGADVP
                     VLVFEDLVCKHRLTPEAIAKLR"
     gene            complement(319..1758)
                     /locus_tag="VFX94_06535"
     CDS             complement(319..1758)
                     /locus_tag="VFX94_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX5767875.1"
                     /translation="MNAVKAVLAVWLASFTVVAATQAQQAVRELPDFTRLVDDQGNAV
                     VNISTTQAPRRSGATPQVPGMEDEEVLEFFRRFIPRQQPGPGARPEARSLGSGFIISA
                     DGYILTNAHVVEGADEINVRFTDKREFKAKIIGADRRTDIAVIKIDATGLPAVRFGDP
                     SKLKVGEWVVAIGSPFGFENTVTAGIVSAKGRSLPQENFVPFIQTDVAINPGNSGGPL
                     FNMRGEVVGVNSQIYSRTGGFMGLSFAIPIDVALDIQKQLREKGRVSRGRIGVVIQEV
                     TRDLATSFGLERPRGALVNSVEKGSPADKAGVEATDIILSFDGKPVESSSDLPRLVGS
                     TRPGSQSTLEVWRKGQMRKLNIVVAELQEDRVAANAPATRAKPQAEAPANRLGIVVGE
                     LSAEQKKGLSLAHGLVVTDVRPESKADVRKGDVLLTLVHKGQHTELKSVEQFNKLLAG
                     LDKNAVITLQVRRGETTAFVTISGLTDKG"
     gene            complement(1774..2721)
                     /locus_tag="VFX94_06540"
     CDS             complement(1774..2721)
                     /locus_tag="VFX94_06540"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HEX5767876.1"
                     /translation="MFRYFIPGVLALAASVAEAQSPETLTWLRKINEATQKLSYTGTF
                     VYQNGARSETSRITRYVDPSTDVERLEVIDGMPREIVRTRDTVRCYLPGLRLVKVDRR
                     TERDFPAVLPERVNALARHYDITLGEKSRIANHECQAVVLTPRDNLRYGYRLYADVKS
                     GMLLRAVTVDASGEPVEQFTFTEFTIGKVTRDMVKARSSSRDWREEDAGAAPAKLAGW
                     GLSAELPGFEKVVELKRRLGESRSAGQVVYSDGLAAVSVFIESLEGRSDPVRTGLASM
                     GAIHIYTREVANHMVTVVGEAPAASVQRIANAVEYRRPQ"
     gene            complement(2721..>2910)
                     /locus_tag="VFX94_06545"
     CDS             complement(2721..>2910)
                     /locus_tag="VFX94_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767877.1"
                     /translation="GWMTFAPQPGPAPLAQVQAEGPKPPAMVPLPNSANDYLLAHQAF
                     SPRVSLAPYIRTVSEQPR"
BASE COUNT          444 a          962 c          999 g          505 t
ORIGIN      
        1 gcgagcccgc cgcacatctg gatgaagcgg tcggcgagcg tcgacttgcc gtggtcgatg
       61 tgcgcgatga tggaaaagtt gcggatccgg gtctgcatcg ggaaaagcgc gcgattttag
      121 cgcagcttgg cgatcgcttc cggggtcagc cggtgcttgc agacgagatc ctcgaacacc
      181 agcaccggca catcggcgcc gtacttgagc cgcagcctgg ggtccgagtc cacgtcgatc
      241 tcgtcgaacg cgatgccgcg cacgcgcagc tcggcggcca tctgctcgca cagatggcag
      301 ccgctgcggc ggtacaacct agcctttgtc agtcagcccg gagatggtga cgaacgcggt
      361 ggtttcacct cgccgcacct ggagcgtgat gaccgcgttc ttgtcgaggc cggcgaggag
      421 cttgttgaac tgctcgaccg acttgagctc cgtgtgctgg cctttgtgca cgagggtgag
      481 cagcacgtcg cctttgcgca cgtcggcctt ggattccggg cgcacgtccg tcaccaccag
      541 gccatgcgcc aggctgagcc ccttcttctg ctcggcggaa agctcgccca ccacaatgcc
      601 gagccggttc gccggcgcct cggcctgcgg cttggcgcgc gtggcgggcg cgttggccgc
      661 gacccgatct tcctgcagct ccgccacgac gatgttgagc ttgcgcatct ggcccttgcg
      721 ccacacttcg agcgtcgact gtgagcccgg gcgcgtggaa ccgacgagcc ggggcaggtc
      781 gctcgagctt tcgaccggct tgccgtcgaa cgagaggatg atgtcggtcg cctccacgcc
      841 cgccttgtcc gccgggctgc ccttctccac cgagttcacc agcgcgccgc gcgggcgctc
      901 gaggccgaac gaggtggcga ggtcgcgcgt cacttcctgg atcaccacgc cgatgcggcc
      961 gcgcgaaacc cggcccttct cgcgcagctg cttctggatg tcgagcgcga cgtcgatcgg
     1021 aatggcgaac gaaaggccca tgaagccgcc ggttcggctg tagatctgcg agttgacgcc
     1081 gacgacctcg ccgcgcatgt tgaagagcgg gccgcccgag ttgcccgggt tgatcgcgac
     1141 gtcggtctga atgaaaggaa cgaagttttc ctgcggcagg gagcggccct tggcgctcac
     1201 gatgccggcg gtcacggtgt tctcgaagcc gaacggcgaa ccgatcgcca ctacccactc
     1261 cccgaccttc agcttgctgg ggtcgccgaa gcgcacggcg ggcaggccgg ttgcgtcgat
     1321 cttgatcacc gcgatgtcgg tgcgccggtc agcgccgatg atcttggcct tgaactcgcg
     1381 cttgtcggtg aagcgcacgt tgatctcgtc cgccccctcg accacatgcg cgttggtgag
     1441 gatgtagccg tcggcgctga tgatgaagcc cgagccgagc gagcgcgcct cggggcgggc
     1501 gccggggcct ggttgctggc gcgggatgaa gcggcggaag aattcgagca cttcctcgtc
     1561 ttccatgccc gggacctgcg gcgtcgcgcc ggagcggcgc ggcgcctggg tggtgctgat
     1621 gttcaccacc gcgttgccct ggtcatcgac caggcgagtg aaatcgggaa gctcgcgcac
     1681 cgcctgctgc gcctgcgtgg cggcaacgac ggtgaaggaa gccagccata cggctagcac
     1741 tgctttgacg gcgttcatct tcttctccct gcgctactgc ggacggcggt actcgacggc
     1801 gttggcgatg cgctgcacgc tcgccgccgg cgcttcgccc accacggtga ccatgtgatt
     1861 cgccacctcg cgcgtataga tatggatggc gcccatgcta gcaagcccgg tgcgcaccgg
     1921 atcgctgcga ccttcgagcg actcgatgaa caccgaaacg gcggccaggc catcggaata
     1981 caccacctgg cccgcggagc gagactcgcc caggcgccgc ttcaactcga ccaccttctc
     2041 gaacccgggc agctcggcgc tgaggcccca gccggcgagc ttcgccggcg ctgcgcccgc
     2101 gtcctcctcg cgccagtcgc gcgaggagga acgcgccttc accatgtcgc gcgtcacttt
     2161 gccgatggtg aactccgtga acgtgaactg ctcgaccggc tcgccggacg cgtccaccgt
     2221 aaccgcgcgc agcagcatgc cgcttttcac gtcggcgtac aggcggtagc cgtagcgcag
     2281 gttgtcgcgc ggcgtgagga cgaccgcctg gcattcgtgg ttagcgatgc ggctcttctc
     2341 gccgagcgtg atgtcgtagt ggcgcgcgag cgcgttgacg cgttcgggaa ggaccgcggg
     2401 gaaatcgcgc tcggtgcgcc ggtcgacctt caccaggcgc aggcccggca gatagcagcg
     2461 caccgtgtcg cgggtgcgca cgatctcgcg cggcatgccg tcgatcacct cgagccgctc
     2521 gacgtcagtg gacggatcga cgtagcgcgt gatgcgcgaa gtctcgctgc gcgcgccgtt
     2581 ctggtagacg aaagtgccgg tgtaggaaag cttttgcgtc gcttcgttga tcttgcgcag
     2641 ccaggtgagc gtctccgggg actgcgcctc ggccaccgag gcggcgagcg cgagcacccc
     2701 cggtatgaag tatcggaaca ttatctgggt tgctcggaca ccgtgcggat gtacggcgcg
     2761 agcgacacgc gcggcgagaa tgcctggtgc gcgagcagat aatcgttcgc ggagtttggt
     2821 aggggaacca tggccggcgg cttcggcccc tcggcttgga cctgcgcgag gggcgccggc
     2881 ccgggctgcg gcgcgaacgt catccaaccg
//
LOCUS       DASUNT010000326         3226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_44323, whole genome shotgun sequence.
ACCESSION   DASUNT010000326 DASUNT010000000
VERSION     DASUNT010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44323"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..361
                     /gene="scpB"
                     /locus_tag="VFX94_06550"
                     /pseudo
     CDS             1..361
                     /gene="scpB"
                     /locus_tag="VFX94_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
     gene            612..1175
                     /locus_tag="VFX94_06555"
     CDS             612..1175
                     /locus_tag="VFX94_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX5767878.1"
                     /translation="MLDGKPLTARAFAPPRLLIYHKPVGELVTRSDPGGRPTVFSSLP
                     PGRWIAVGRLDLNSSGLLLFTDSGELANRLMHPRYGLEREYSARIRGSLSAAEERRLL
                     QGVPLDGIPARFESIRSEREGGGTNRWYRVVLKEGRNREVRRLFEALGHPVSRLVRLR
                     YGPVALPADLPAKSWRELPDLAILQRL"
     gene            1205..1636
                     /gene="rimP"
                     /locus_tag="VFX94_06560"
     CDS             1205..1636
                     /gene="rimP"
                     /locus_tag="VFX94_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX5767879.1"
                     /translation="MSLEKVVEPAVSGLGFELVDVQASNGGRLLRLFIDKPGGITVED
                     CASVSRHLTRVLAVEGIDYERLEVSSPGLDRLLRKGTDFVRFAGRKAEVRMRTPDASG
                     RRRFVGVLRGAEAGRVQLELDGQTLGLELDDVERARLVPEL"
     gene            1647..3143
                     /gene="nusA"
                     /locus_tag="VFX94_06565"
     CDS             1647..3143
                     /gene="nusA"
                     /locus_tag="VFX94_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX5767880.1"
                     /translation="MSFGRELLLQADALAREKNVTRDIVFSALELALASALKKRYLED
                     VDVRVAIDRATGNYNAFRRWKIVPDGTVEIAAQQIPVSEAVKKNPAAKAEEFIEEPIE
                     PIDPRRIGAQTAKQVLLQRIRDAEREQILNDFLSRGDELVTGTVKRMERGNAIIESGR
                     LEASLPRDHMIPKENLRVGDRVRAWVKQIDRQARGPQLILSRTAPQFIMKLFELEVPE
                     IEEGLLEIKSAARDPGVRAKIAVFTNDKRIDPIGTCVGMRGSRVQAVTQELAGERVDI
                     VLWSADPAQFVIGALAPAEVSSIVVDEEKHAMDVVVDEENLAVAIGRSGQNVRLASEL
                     TGWQINLMTEEESNKKQQEESGRIKSHFMEKLDVDEEVANILIEEGFSTLEEIAYVPI
                     NELKEIDAFDEDTVNELRTRARNALLVAAIANEEKVEGLDPELLKLEGMTTQLAAKLG
                     ENGIKTRDDLADLAVDELSEMTGMEAEAAKKLIMAARAHWFNDSKEAA"
BASE COUNT          580 a         1042 c         1110 g          494 t
ORIGIN      
        1 gtggcgcggc cgcagcgtgg agctcgtgag cctgtcgagc ggctggcgtt tccagacgcg
       61 ccccgagttc cagccctatc tggagcgcct gttccccgag cgcgcgccgc gctactcccg
      121 cgcggtgatg gagacgctgg cgatcattgc ctaccggcag ccggtaacac gcggcgatat
      181 cgaggacgtg cgcggggtcg gggtctcgac ccagatcatc caggcgctcg agacgcgggg
      241 ctggatcgac gtcgtcggcc accgcgaaac cccgggccgc ccagcgcttt acgccaccac
      301 caccaagttc ctcgacgatc tggggctgcg ctcgctcgag gagctgccgc cgctcgaaga
      361 aatcgccaag accctcgagc tcgaggttgt ttccactgcg ccccctgccg aagcggcggt
      421 ccgagaaccc gaagcccaac ccgaagccca ccaggccacc ggcaccgacg gctgagcgcc
      481 tgcagaagct gctcgcggcg gcggggctcg ggtcgcgccg cgagatcgag ggctggatct
      541 cggccgggcg gctgagcctt aacggtcggc tcgcgaggct cggcgaccgc gcgggcccct
      601 cggactccat aatgctggac ggcaaaccgc tcaccgcccg ggcgttcgcg ccgccgcggc
      661 ttctgatcta ccacaagccg gtcggcgagc tcgttacgcg cagcgatccg gggggcaggc
      721 cgaccgtttt ctcttccctc ccgcctggac gctggatcgc ggtcgggcgg ctcgacctca
      781 atagctcggg cctgcttctc tttaccgact ccggcgagct tgcgaaccgc ctgatgcacc
      841 ccagatacgg tcttgagcgc gaatactcgg cgcgcattcg cggctccctt agcgccgctg
      901 aagaaaggcg cctcctgcag ggcgtgccgc tcgacggcat cccggcccga ttcgaaagca
      961 tccggagcga gcgcgagggc gggggcacca atcgctggta ccgcgtcgtc ctcaaggaag
     1021 gccgtaaccg cgaagtgcgg cgccttttcg aggcgcttgg ccatccggtc agccgcttgg
     1081 tgcggctgcg ctacggcccg gtcgcgctgc ccgcggatct gcccgccaaa agctggcgag
     1141 agctgccgga tctggctata cttcaaaggc tttagagaga gtgggcttcg gcccattttt
     1201 tgttttgagc ctcgaaaaag tagtggagcc ggcggtgagc ggactgggtt tcgagctggt
     1261 cgacgtccag gcgtcgaacg gcgggcgcct gttgcgcctc ttcatcgaca agcccggggg
     1321 tatcacggtc gaggattgcg cgagcgtcag tcggcacctg acgcgggtgc tcgcggtcga
     1381 gggtatcgac tatgagcggc tcgaggtgtc gtcgccggga ctcgaccggc tgctgcgcaa
     1441 ggggacggat ttcgtgcgct ttgccggccg caaggccgaa gtgcgcatgc gcacgcccga
     1501 cgcatcggga cggcgccgat tcgtcggggt gctgcgcggc gccgaagccg ggcgcgtgca
     1561 gctcgagctc gacgggcaga cgctcggcct cgagctcgat gacgtggagc gggcgcggct
     1621 cgtgcccgaa ctgtgagaga gggtgaatga gtttcggtag agaactgctg ctgcaagccg
     1681 atgcgctggc gcgcgagaag aacgtgacgc gggacatcgt tttctcggcg ctcgagctcg
     1741 cgctcgcctc ggcgctcaag aagcgctacc tcgaggatgt cgacgtgcgg gtcgcgatcg
     1801 accgcgccac cggcaactac aacgcattcc ggcgctggaa gatcgtgccc gacggcacgg
     1861 tggagatcgc cgcgcagcag atcccggtgt ccgaggcggt gaagaagaac ccggcggcaa
     1921 aggccgagga gtttatcgag gagccgatcg agccgatcga tccgcgccgc atcggcgcgc
     1981 agaccgccaa gcaggtgctc ctgcagcgta tccgcgacgc cgagcgcgag cagatcctga
     2041 acgacttcct ttcgcgtggc gacgagctcg tcaccggcac ggtgaagcgc atggagcggg
     2101 gcaacgcgat tatcgagtcc gggcggctcg aggcttcgct gcctcgagac cacatgattc
     2161 ccaaggagaa ccttcgggtt ggcgaccgcg tgagggcatg ggtaaaacag attgacaggc
     2221 aagcaagagg tccgcaactc atcctctcgc gcaccgcgcc tcagttcatc atgaagctct
     2281 tcgagctcga ggtgcccgag atcgaggagg ggctgctcga gatcaagtcc gcggcgcgcg
     2341 accccggcgt gcgggcgaag atcgccgtct tcaccaacga caagagaatc gacccgatcg
     2401 gcacgtgcgt cggaatgcga ggctcgcgcg tgcaggcggt gacgcaggag ctcgccggcg
     2461 agcgcgtgga catcgtgctc tggtcggccg acccggcgca gttcgtgatc ggcgcgctcg
     2521 cgcctgcgga ggtcagttca atcgtcgttg acgaagaaaa gcatgccatg gacgtggtgg
     2581 tcgacgagga aaacctcgcc gtcgcgatcg gccgcagcgg ccagaacgtg cgcctcgcct
     2641 ccgagctcac cggctggcag atcaacctca tgaccgagga ggaatcgaac aagaagcagc
     2701 aggaggagag cgggcgcatc aagagccact tcatggaaaa gctcgacgtc gacgaggaag
     2761 tcgccaatat cctcatcgag gaagggttct ccaccctcga agagatcgca tacgtgccga
     2821 tcaacgagct gaaggagatc gacgctttcg acgaggacac tgtgaacgag cttcgcaccc
     2881 gcgcgcgcaa cgcgctcctc gtcgcggcga tcgcgaacga ggaaaaggtc gagggcttgg
     2941 accccgagct cctcaagctc gaaggcatga cgacccagct cgccgcgaag ctcggcgaga
     3001 acggcatcaa gacgcgcgac gatctcgccg acctcgcggt cgacgagctc tccgagatga
     3061 ccggcatgga agccgaggct gcgaaaaagc tcatcatggc ggcccgtgcg cactggttca
     3121 acgacagcaa ggaggccgcg taagtggcgc agacgagcgt agcgcagttc gcgagcgagc
     3181 ttaaggtccc gccgtccgtc ctgctcgagc agctgcgcgc tgccgg
//
LOCUS       DASUNT010000327         7249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_396083, whole genome shotgun sequence.
ACCESSION   DASUNT010000327 DASUNT010000000
VERSION     DASUNT010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396083"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFX94_06570"
     CDS             complement(<1..215)
                     /locus_tag="VFX94_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5767881.1"
                     /translation="MRVFAARGLTKTYHMGETEIHALRGVDLEISAGEFIVLLGPSGS
                     GKSTLLNILGGLDTPTSGQIVFKEEEEL"
     gene            270..1232
                     /locus_tag="VFX94_06575"
     CDS             270..1232
                     /locus_tag="VFX94_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX5767882.1"
                     /translation="MPHREIVASHLVERAARARGHETAGAVLTRLAKEKPASVELVLV
                     VDDHHRLQGVVPLAKLFAAAAQAKLKDMVAHGFPTVRPETDQEHAASLALHHGVDALP
                     VVDAKARALGVMPSQALLQVLRREHVEDLHRLAGIQREAAQARHAIEDPPLRRARHRL
                     PWLLVGLAGSALATAAMTAFESTLSSTIAVAFFVPGIVYLADAIGTQTEAIAVRRLSL
                     TRAGLARLLAGELRTGMLLGGILGVASFFPVWLIFGHLPLAAAVATAIFAAGTMAAGL
                     GLLLPWWIARTGRDPAFGSGPLATVIQDVLSLLVYLGVVRAFGV"
     gene            1236..3848
                     /locus_tag="VFX94_06580"
     CDS             1236..3848
                     /locus_tag="VFX94_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5767883.1"
                     /translation="MHSVPANEALAQLDSGAQGLSSAQAAERLRRHGPNALPEAKRRH
                     PLARFAAQFHNVLIYFLLASALGAAILGHAIDAGAIVAVVLVNAFVGFIQEGKAEKAL
                     AAMQRMIAPQASVLRDGARASVPAASLVPGDVVLIEPGDRVPADLRLLRARALRIDEA
                     ILTGESIPAAKNETPAPAAADLGARHSMAYSGTFVAAGQGSGVVTATGPATEIGRISA
                     LLASVEPLTTPLIRQMNRFAHRFTWVAIAGSAALFAFAVLVRDFPWMQALLGVVALAV
                     SVVPEGLPAVITITLAIGVRRMAARNAVIRRLPAVETLGATSVICSDKTGTLTRNEMT
                     VRRVVTVEHEAAVSGSGYAPEGQIEAVGHDDDAACVASALPLIRCGLLCNDARLRLRD
                     GTWAVEGDPMEGALIALAMKAGLNPEHVRAEWPRLDEVPFDAAYRFMATLHRGPDGEL
                     LLFVKGAPEELLARCGMPDDATWLARIARAASAGERVLGFAVKQCESSSRLDVPSIRE
                     LRFLGIAGFIDPPRPEALAAIAQCKSAGIAVKMITGDHARTAAAIAAQLGLPAEAVYA
                     RATPEDKLRLVRALQAEGAIVAMTGDGVNDAPALKQADVGVAMGIKGTEAAKEAAEMV
                     LADDNFASIVAAVHEGRVVHDNIRKVVAWTLPTNGGEALTVIAALVFGLVLPMTAAQI
                     LWVNLVAESALGLALAFEPAEPGVMRRPPRRRDEPLLSSFLLWRVVVVSLLFAAVSLG
                     MFFAALGLGRDLETARTIVVNALVVLQVFYLFNVRYLHMPSLTLRGALGTPAVLWALV
                     AIVAAQLLLTYAPIMQHWFATRSLSLVDGAVVIACGAAFMFLLEAEKLVLRRFGFLEK
                     PPSGTPVPGPSRAG"
     gene            complement(3808..4251)
                     /locus_tag="VFX94_06585"
     CDS             complement(3808..4251)
                     /locus_tag="VFX94_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5767884.1"
                     /translation="MFKHILIPTDGSPVSAKAVKAGIAFAKRSGGRVTGYYAIEPVPM
                     RLYGEGYVVDRQMVAEFERRARDAAKKHVEAVARAAAKAGVKCDTLVETARTPYEGII
                     EAARKRRCDLILMASHGYRGLMRLTLGSVTDKVIQLSKVPVLVYR"
     gene            complement(4283..4993)
                     /locus_tag="VFX94_06590"
     CDS             complement(4283..4993)
                     /locus_tag="VFX94_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5767885.1"
                     /translation="MPQKFEVNCASCNLRELCLPAGLCAEDLERVENIVYARRRVKRG
                     EALFNAGAEFGAVYAIRSGFFKTSVLDGDGREQVTGFFMGGELVGLDGLGCGRYNGTA
                     IALEDSEVCVLPYALIEEMSREVPALQRHLHAVLAREIVRDHGVMLLLGSMRAEERLA
                     TFLLNLSRRFVRRGYSASDFHLRMTREELGSYLGLKLETVSRLFSQFQTERLIDVQQK
                     HVCILDVPGLERVLGTKH"
     gene            complement(5052..5423)
                     /locus_tag="VFX94_06595"
     CDS             complement(5052..5423)
                     /locus_tag="VFX94_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5767886.1"
                     /translation="MEIFLVEDSSLLRQRLAELLGAIPGASLVGEAERADEAIRDILS
                     LRPDVVVLDLSLAGGTSGFEVLREVCRKAPEIDVYMLSNFVAAPYRELAERLGARDFF
                     DKSSELERARRVLTERAAAVH"
     gene            complement(5493..5858)
                     /locus_tag="VFX94_06600"
     CDS             complement(5493..5858)
                     /locus_tag="VFX94_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5767887.1"
                     /translation="MKVFLVEDAPLLRERLEAMLNAIPGVQTVGHAAQAEPAVEGIVR
                     AGADAVVLDIQLAKGSGFDVLRALQSRAPHIAVYVLTNFATEGYRKAAERLGARGFFD
                     KSNDFGVLREALAAGAAGN"
     gene            complement(6023..6640)
                     /locus_tag="VFX94_06605"
     CDS             complement(6023..6640)
                     /locus_tag="VFX94_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5767888.1"
                     /translation="MMRVLIADDHSIVRDGLKRILAATPDVQVAAEAATGDEALRLVK
                     ASDYDVVMLDMSMPGLSGIDLIKRLKLERPKLKILVLSMHGESQYAARALKAGAAGYL
                     TKDSAAEVMVGALRKIAAGGVHIGEAAAATLLQAGDKPPHESLSDREFEVLRLLVEGL
                     GPTEIGERLHLSVKTVSTHKTRILDKLGVGSTAELVRYALEHRLL"
     gene            complement(6637..>7249)
                     /locus_tag="VFX94_06610"
     CDS             complement(6637..>7249)
                     /locus_tag="VFX94_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5767889.1"
                     /translation="HIARELHDELGQLLTALKMDLAWLRERLGPDTDLSARAAEMGAL
                     LDRTVGSTRRISADLRPLMLDDLGLADAAAWLVEDFAKRSGISCRIDVSQEVQGLSRA
                     VATAVYRAVQESLTNIARHSGAKNAWVLLGATDGVVQVEIEDDGRGIAPEDLAKARSL
                     GLKGMRERITFLGGSLDIGRAPRGGTRVKLTVPLRGLVTETAG"
BASE COUNT         1055 a         2511 c         2499 g         1184 t
ORIGIN      
        1 agctcctcct cctccttgaa aacgatctgc cccgaagtcg gggtgtccag accgccgaga
       61 atgttgagga gcgtcgattt gccgctgccc gaaggaccga ggagcacgat gaactcgcca
      121 gccgagatct cgagatctac cccgcgcagc gcgtgaatct cggtctcgcc catgtggtag
      181 gtcttggtaa ggccgcgggc ggcaaagacg cgcatttgac cattgtcgct cagcgtgccg
      241 gcaggctctt attagggtga aataacggga tgcctcaccg cgaaatcgtc gcgtcgcacc
      301 tggtggagcg cgctgcgcgc gctcgcggcc acgagaccgc gggcgccgtg ctcacgcgcc
      361 tcgcaaaaga aaagccggcg agcgtcgagc ttgtcctcgt cgtggacgac caccaccgtt
      421 tgcagggcgt cgtgccgttg gcgaagcttt tcgccgccgc cgcgcaggcg aaactaaagg
      481 atatggttgc gcatggcttt cccaccgttc gccccgaaac ggaccaggag cacgccgctt
      541 cgctcgcgct ccaccacggc gtagacgcgc tgcccgtggt cgatgccaag gcccgggcgc
      601 tcggcgtgat gccctcgcag gcgctccttc aggtgctgcg ccgcgagcac gtggaggacc
      661 tgcaccggct cgccggcatc cagcgcgaag ccgcgcaggc gcgtcacgcg atcgaggacc
      721 cgccgctgcg gcgcgcgcgc catcggctgc cctggcttct cgtcggcctc gcgggcagcg
      781 cgctcgccac cgccgccatg acggcgttcg agtccactct gagctccacg atcgcggtgg
      841 ccttcttcgt gcctggaatc gtgtatctcg ccgacgcgat cggcacccag acggaggcga
      901 tcgcggtgcg acgcctctcg ctcacgcgcg caggcctcgc gcgattgctg gcaggggagt
      961 tgcgcaccgg catgctgctc ggagggatcc tcggcgtcgc gagcttcttc ccggtgtggc
     1021 tcattttcgg ccatctgccg ctcgctgccg cggtggccac cgcaatcttt gccgcgggga
     1081 cgatggcggc gggcctgggg ctgctgctgc cctggtggat tgcgcgcacc gggcgcgacc
     1141 cggcattcgg cagcggcccg ctcgcgaccg tgatccagga cgttctcagc ctgcttgtct
     1201 acctcggcgt ggtgcgcgcc ttcggggtct aggcgatgca ttccgtcccg gcgaacgaag
     1261 cgctcgcgca gctcgatagc ggcgcgcagg gcctgagctc ggcgcaagcc gcggagcggt
     1321 tgcgtcgcca tgggcccaac gcgctcccgg aggcgaagcg gcgccacccc ctggcgcgct
     1381 tcgccgcgca gttccataac gtgctgatct atttcctgct cgcctcggcg ctcggcgcgg
     1441 cgatcctcgg gcacgccatc gacgccggtg ccatcgtcgc agtagtgctg gtgaacgcct
     1501 tcgtcggctt catccaggaa ggcaaggccg aaaaggcgct cgccgcgatg cagcgcatga
     1561 tcgcgccgca ggcgagcgtg ctgcgcgatg gtgcgcgggc gagcgttccc gcagcgagcc
     1621 tggtgcccgg agatgtcgtg ctgatcgagc ccggcgaccg ggttccagcc gacctgcgcc
     1681 tgcttcgcgc gcgcgcactg cggatcgacg aggcgatcct cacgggcgag tcgatcccgg
     1741 cggcgaaaaa cgagacgccc gcgccggcgg ctgccgatct cggcgcgcgg cacagcatgg
     1801 cctactccgg caccttcgtc gcggccggac agggcagcgg cgtggtgacg gcgactggcc
     1861 cggccaccga gatcggccgc atcagcgcgc tgctcgcgtc ggtggagccg cttacgacgc
     1921 cgctcattcg ccagatgaac cgcttcgcgc atcgcttcac ctgggtggcg atcgcgggct
     1981 ccgcggcgct tttcgcgttc gcggtgctgg tgcgcgactt cccatggatg caggcgctgc
     2041 tcggcgtggt ggcgctcgcg gtcagcgtgg tgcccgaagg cttgccggcg gtaatcacga
     2101 tcaccctcgc gatcggagtg cgccgcatgg cggcgcgcaa cgccgtgatc cgccgcctgc
     2161 ccgctgtcga gacgctgggc gcgacctcgg tgatctgctc cgacaagacc ggcacgctga
     2221 cgcgcaacga aatgacggtg cggcgcgtcg tcaccgtcga gcacgaagct gccgtctcgg
     2281 gctcgggtta cgcacccgag gggcagatcg aggccgtcgg gcacgacgac gacgcggcgt
     2341 gcgtcgcctc ggcgttgccg ctcattcgct gcgggctgct gtgcaacgac gcccggctgc
     2401 gcttgcgcga cgggacctgg gcggtcgagg gcgacccgat ggagggagcg ctgatcgccc
     2461 ttgcaatgaa ggccgggctg aacccggagc acgtgcgcgc cgagtggccg cgcctggacg
     2521 aagtgccgtt cgacgcggct taccgcttca tggcgaccct gcaccgcggc ccggacggcg
     2581 agctgctgct cttcgtcaag ggcgcacccg aggagctgct cgcgcgctgc ggcatgccgg
     2641 acgacgccac gtggctcgcg cggatcgcgc gcgcggcctc ggccggcgag cgcgtcctcg
     2701 gttttgcggt gaagcaatgc gagtcctcgt cgcggctcga cgtgccatcg atccgagagc
     2761 tccgcttcct cggcatcgcg ggcttcatcg atccgcctcg ccccgaggcg ctcgctgcga
     2821 tcgcacagtg caagagcgcc ggcatcgcag tgaagatgat caccggcgac cacgcgagaa
     2881 ccgcggccgc gatcgcggcg cagctcggcc tgcccgccga agcggtctac gcgcgggcga
     2941 ctccggagga caagctgcga ctggtgcgtg cgctccaggc cgaaggcgcc atcgtcgcca
     3001 tgaccggcga cggcgtgaac gacgcgcccg ccttgaagca ggccgacgtc ggcgtcgcca
     3061 tggggatcaa gggcaccgag gcggcaaagg aggcggccga gatggtgctc gccgacgaca
     3121 acttcgcctc catcgtggcc gcagtgcacg agggacgagt ggtgcacgac aacatccgca
     3181 aggtggtggc gtggaccctt cccacgaacg gcggcgaggc gctcacggta atcgccgccc
     3241 tcgttttcgg actggtgctg ccgatgaccg ccgcccagat cctgtgggtg aacctcgtcg
     3301 ccgaatcggc gctcggcctg gctctcgcct tcgagcccgc cgagcccggc gtgatgcgcc
     3361 ggccgccgcg gcgccgcgac gagccgctgc tctcgagctt tcttctctgg cgcgtggtcg
     3421 tggtgtcgct acttttcgcg gccgtgtccc tcggcatgtt cttcgcggcg ctcgggcttg
     3481 gccgggacct ggagaccgcg cgaaccatcg tggtaaacgc gctcgtcgta ctgcaggtgt
     3541 tctatctctt caacgtgcgc tacctgcaca tgccctcgct cacgctgcgc ggcgcgctcg
     3601 gcacgccggc ggtgctgtgg gcgctcgtcg ccatcgtggc ggcgcagctc ctgctcacgt
     3661 acgcgccgat catgcagcac tggtttgcga cccggtcgct ctcgctcgtc gacggcgccg
     3721 tcgtcatcgc gtgcggcgcc gcgttcatgt tcctgctcga ggcggaaaag ctcgtgctgc
     3781 gccgcttcgg cttcctggag aagccgccta gcggtacacc agtaccggga ccttcgagag
     3841 ctggatgacc ttgtcggtga cgctacccag cgtgaggcgc atcaagccgc ggtagccgtg
     3901 cgacgccatg aggatcaggt cgcacctgcg cttgcgcgcc gcttcgatga tcccttcgta
     3961 gggcgtgcgc gccgtttcca ccagcgtgtc gcacttcacg ccggccttgg ccgcggcgcg
     4021 tgccaccgcc tcgacgtgct tcttcgcggc gtcgcgcgcg cggcgctcaa attcggcgac
     4081 catctgccgg tcgacgacat agccttcgcc gtacaggcgc atcggtaccg gctcgatcgc
     4141 gtagtagccg gtgaccctgc cgccgctacg tttggcgaac gcgatgcccg ccttaaccgc
     4201 cttggcggaa acgggcgagc cgtccgtggg aatcaggatg tgcttgaaca tgcgctctcc
     4261 tccctggatg gatcgcgcaa ttctagtgct ttgtccccag cacgcgctcg aggccgggca
     4321 cgtcgaggat gcagacatgc ttctgctgca cgtcgatcag ccgctcggtc tggaactggg
     4381 agaagaggcg gctcaccgtc tcgagcttga gccccaggta gctgccgagc tcctcgcgcg
     4441 tcatgcgcag gtgaaagtcg gaggccgagt agcctcggcg cacgaagcgc cgggagaggt
     4501 tgagcaggaa cgtggcgagg cgctcctcgg cgcgcatcga gcctagaagc agcatgacgc
     4561 cgtggtcgcg cacgatctcg cgcgcgagca ccgcatgcag gtggcgctgc agcgccggaa
     4621 cctcgcgcga catttcctcg atcagggcat acggcaggac gcagacttcg ctgtcttcga
     4681 gagcgatcgc ggtgccgttg tagcggccgc agccgaggcc gtcgaggccg acgagctccc
     4741 cgcccatgaa gaaaccggtc acctgctcgc gtccgtcgcc gtcgagcacg ctcgtcttga
     4801 agaagccgct gcggatcgca tacacggcgc cgaactccgc gcccgcgttg aacagcgcct
     4861 cgccgcgctt cacacgcctg cgtgcgtaga cgatgttctc cacgcgctcg aggtcctcgg
     4921 cgcacaggcc ggcgggcagg caaagctcgc gcaggttgca gctcgcgcag ttcacttcga
     4981 atttttgcgg catgggcttg aacctcggtc ggatggcgat gacggcggct tgcatgtggc
     5041 tgggctccct ttcagtgcac ggcggcggcg cgctcggtca gcacgcgacg cgcgcgctcg
     5101 agctcactgc tcttgtcgaa gaaatcgcgc gcacccagcc gctcggcgag ctcgcggtag
     5161 ggcgcggcga cgaagttcga aagcatgtag acgtcgatct cgggggcctt gcggcacacc
     5221 tcgcgcagca cctcgaagcc gctcgtcccg ccggcgagcg aaagatcgag caccaccacg
     5281 tcggggcgca ggctgaggat gtcgcggatc gcttcgtcgg cgcgctcggc ttcgcccacc
     5341 aggctcgcgc ccgggatcgc gccgagcaac tcggcgagcc gctgcctgag tagcgacgag
     5401 tcctcgacga gaaagatctc catgccggca gtgtgccgac gcggcccggc gcgccctatc
     5461 ggcgctcgct gatattcagg gtaggagctt tcctaattgc ccgccgcgcc ggccgcgagc
     5521 gcttcgcgca ggaccccgaa gtcgttggac ttgtcgaaga acccgcgggc gcccaggcgc
     5581 tcggccgcct tgcgatagcc ctcggtggca aagttggtga gcacatagac cgcgatatgc
     5641 ggcgcgcggc tctgaagcgc gcgcagcacg tcgaagccgc ttcccttcgc gagctggatg
     5701 tcgagcacca cggcgtccgc gcccgcccgc acgataccct cgacggcggg ctcggcctgc
     5761 gccgcgtggc ccaccgtctg cacgcccgga attgcgttca gcatcgcctc aaggcgctcg
     5821 cgcagcagcg gcgcgtcttc gaccaggaag accttcactg cgcgagtatg cgggtgcttt
     5881 gctccccggc tggccgccgc cctgtaggcg gtggccgatt tcggggtagg aattttccta
     5941 ggagtgcgcg tgtgcaccat ggcggaccat ggtgcagcgc gctgcatttc agctcttgat
     6001 ccaggtcaat caggccaaac ggctaaagca ggcggtgctc cagggcgtag cgcacgagct
     6061 cggccgtgct gccaacgccg agcttgtcca ggatgcgggt cttgtgcgtg cttaccgtct
     6121 tcaccgagag gtggaggcgc tcgccgatct cggtcgggcc gagtccctcc acgagcagcc
     6181 gcagcacctc gaactcgcgg tccgagagcg actcgtgcgg cggcttgtct cccgcctgca
     6241 gcagggtggc ggccgccgct tcgccgatgt gcacgccgcc cgcggcgatc ttgcgcagcg
     6301 cgccgaccat cacctcggcg gcgctgtcct tggtaaggta gcccgcggcg ccggccttga
     6361 gcgcgcgcgc ggcgtactgg ctctcgccgt gcatcgagag gacgagaatc ttgagcttcg
     6421 gccgctcgag cttgagccgc ttgatgagat cgatccctga aagccccggc atcgacatgt
     6481 cgagcatcac gacgtcgtag tcgctcgcct tcacgagacg cagcgcctca tcgccggtgg
     6541 ccgcctctgc cgcgacctgc acatcgggcg tcgcggcgag gatgcgcttg agcccatcgc
     6601 gcacgatgct gtggtcgtcg gcaatgagaa cgcgcatcag ccggccgttt cggtcacgag
     6661 cccgcgcagc ggcacggtga gcttgacgcg cgtaccgccg cgcggcgcgc gcccgatgtc
     6721 gagggagccg ccgagaaagg tgatgcgctc gcgcatgccc ttgaggccaa gggagcgcgc
     6781 cttggcgagg tcctcgggcg cgatgccgcg gccgtcgtcc tcgatctcga cttgcactac
     6841 gccgtcggtc gcgccgagca gcacccaggc gttcttcgcg ccggaatggc gtgcgatatt
     6901 ggtcagcgat tcctggaccg cacggtaaac cgcggtggcg accgccctgg acaacccctg
     6961 cacttcctgg gacacgtcga tacggcagga gatgccggag cgcttggcaa aatcctcgac
     7021 gagccaggcg gcggcgtcgg cgaggccgag atcgtcgagc atgagcggcc gcaaatcggc
     7081 cgagatgcgg cgcgtggagc cgaccgtgcg atccaggagc gcgcccatct ccgcggcgcg
     7141 cgcggagaga tcggtatccg ggcccagccg ctcgcgcagc cacgcgagat ccatcttgag
     7201 cgcggtgaga agctggccaa gctcgtcgtg cagctcgcgc gcgatgtgg
//
LOCUS       DASUNT010000328         2625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_396102, whole genome shotgun sequence.
ACCESSION   DASUNT010000328 DASUNT010000000
VERSION     DASUNT010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396102"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="VFX94_06615"
     CDS             <1..142
                     /locus_tag="VFX94_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S ferredoxin"
                     /protein_id="HEX5767890.1"
                     /translation="DLLDKAWGLEATSRLSCQAKIADGDLVIEIPKYTINYEREGGGE
                     KK"
     gene            139..333
                     /gene="iscX"
                     /locus_tag="VFX94_06620"
     CDS             139..333
                     /gene="iscX"
                     /locus_tag="VFX94_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEX5767891.1"
                     /translation="MRWTDTQEIAIALADAHPDEDPKAVNFVDLYKWVMALPGFGDDP
                     KRCGEKILEAIQQAWIEETE"
     gene            complement(334..540)
                     /locus_tag="VFX94_06625"
     CDS             complement(334..540)
                     /locus_tag="VFX94_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767892.1"
                     /translation="MPTRYWLALFFLSSLVLSGCSSRSAAAGGTGGNCVLASKAHREG
                     ALTCQQGTGYRCESGVWNSTFKPC"
     gene            548..2038
                     /locus_tag="VFX94_06630"
     CDS             548..2038
                     /locus_tag="VFX94_06630"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX5767893.1"
                     /translation="MPEAQQGTRPRIAILGLHLESNAFAPPSGEAQFRSLCYVQGEAI
                     TREARKDPSALPAEVPAFYAEMDRLSPWTAAPILVTAAEPGGPVDEIFFQQTLAAMRE
                     GLAAAGSLDAVYISNHGGMVSTGGPDPDGELYEMVRKQVGAQVPVVATVDLHANISER
                     MVANVDALVSYRTNPHMDQRDRAVDAARLLRKFLQGTSSCRAFVRLPLTPASVTLLTR
                     EGPYADAIAYGQTKLGGGVLDVSVVGGFVFSDTPKNGVAVVVNGEAGADVRGTALDIA
                     ERIWSERERFVKRLTSMEDAIASRQGGFVWSDAGDNPGGGGTGQTTALLKMLYESGAR
                     NVLYGLFFEPEIAAAARGREEMEARFTEGFSVPARVLKTTDGRCVGRRGIWAGRALEL
                     GPTCALQIGGVTAVCVSRRKQCADPVFFEMHGLDIRAARTVVVKSRGHFRGGFDEFFP
                     PERVVEVDTPGLTSPVLERLDFKGLPRPVFPLDAQAAWTPRVAVYE"
     gene            2031..2360
                     /locus_tag="VFX94_06635"
     CDS             2031..2360
                     /locus_tag="VFX94_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767894.1"
                     /translation="MSSLLCLAGEGIATTIFAAALHIAWTPADGIAREESWHVRQGYF
                     EFFAARIRKERDWEHYWPARPPQARLILDRRGLRQDYVICGEMWCTPLEKLVPRARAP
                     VVQIAPC"
     gene            complement(2357..>2625)
                     /locus_tag="VFX94_06640"
     CDS             complement(2357..>2625)
                     /locus_tag="VFX94_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX5767895.1"
                     /translation="VESASAEEIFARPNHPYTQALLKQVPRLDTRKTQFKPIAGEIPS
                     PLSPPAGCHFHPRCPHALPRCREEAPRLRKVAADHLSACHLNGA"
BASE COUNT          433 a          861 c          931 g          400 t
ORIGIN      
        1 ggacctgctc gacaaggcgt ggggcctgga ggcgacctcg cgcctttcgt gccaggcgaa
       61 gatcgccgac ggagatctcg tcatcgagat tccgaagtac accatcaact acgagcgcga
      121 gggaggaggg gagaagaaat gagatggacc gacacgcagg agatcgccat cgcgctcgcc
      181 gacgcccatc ccgacgagga cccgaaggca gtgaacttcg tcgacctgta caagtgggtc
      241 atggcgctgc cgggcttcgg cgacgacccc aagcgctgcg gcgaaaagat cctcgaggct
      301 atccagcagg cctggatcga ggagacggaa tgactagcag ggcttgaagg tgctgttcca
      361 gacgccgctt tcgcagcgat accctgttcc ttgctggcag gtgagcgcgc cttccctgtg
      421 agccttggat gcgagcacgc agttgcctcc cgtgccgccg gcggcggccg agcggctgct
      481 gcagccggag agcaccaggc tggaaaggaa gaaaagcgcg agccagtacc tcgtaggcac
      541 ctcctgaatg cctgaggccc agcaaggaac gcgcccgcgc atcgcgatcc tgggcctcca
      601 tctggagagc aacgctttcg cgccgccgag cggcgaggcg cagttccgct cgctgtgcta
      661 cgtgcaagga gaggcgatca cgcgggaagc gcgcaaggac ccctcggcgc tgcctgccga
      721 agtgccggcc ttctacgccg agatggaccg gctcagtccc tggaccgcgg ctccgatcct
      781 tgtaacggcg gcggaaccgg gcgggccggt ggacgagata ttcttccagc aaaccctcgc
      841 agcgatgcgc gagggccttg cggccgccgg cagcctcgac gccgtctaca tcagcaacca
      901 cggcggcatg gtctccaccg gcggacccga cccggacggc gaactctacg aaatggtgcg
      961 caagcaggtc ggcgcccagg tgccggtggt cgcaacggtg gatttacacg cgaacatctc
     1021 cgagcgcatg gtggccaatg tggatgccct cgtctcgtac cgcaccaacc cgcacatgga
     1081 ccagcgggac cgggcggtgg atgccgctcg attgctacga aaattcctcc aaggcacttc
     1141 ctcatgcagg gcgttcgtcc ggctgccgct gacgccggct tcggtgacat tgctcacaag
     1201 agaggggccg tacgccgacg cgatcgccta cggccagacc aagctcggtg gcggtgtgct
     1261 cgacgtgtcg gtcgtcggcg ggttcgtctt cagcgacacg ccgaagaacg gcgtcgcggt
     1321 ggtggtgaac ggcgaggcgg gcgccgacgt gcgcggcacg gcgctcgaca tcgcggagcg
     1381 catctggagc gagcgcgaac gcttcgtgaa gcgcctgacc tccatggagg acgcgatcgc
     1441 gagccggcaa ggcggcttcg tctggtccga tgccggcgac aacccgggag gcggcggcac
     1501 gggccagacg actgcgctgc tgaaaatgct gtacgaatcc ggagcgcgca acgtccttta
     1561 cggcctcttc ttcgagcccg agatcgcagc cgcagcccgc gggcgcgagg aaatggaggc
     1621 tcgcttcacc gaaggcttca gcgtccccgc ccgggtcctg aagaccaccg acggccggtg
     1681 cgtcggccgg cgcggcatct gggcgggccg cgcgctcgag ctcgggccga cctgcgcgct
     1741 gcagataggc ggcgtcacgg cggtgtgcgt cagccgccgc aagcagtgcg ccgacccggt
     1801 gttcttcgag atgcacggcc tcgacatccg cgcggcgcgc accgtggtgg tcaagtcccg
     1861 cggccacttc cgcggcggct tcgacgaatt ctttccgccc gagcgggtgg tggaggtgga
     1921 taccccgggg ctcacctcgc cggtgctcga gcgcctcgac ttcaagggcc tgccgcgccc
     1981 ggtgtttccg ctcgatgcgc aggcggcctg gacgccgcgc gtcgccgtgt atgagtagct
     2041 tgctctgcct cgcgggcgaa ggcatcgcaa ctacgatctt tgccgcggca ctgcatatcg
     2101 cctggactcc ggcggacggc atcgcgcgcg aggagtcgtg gcacgtcagg cagggctatt
     2161 tcgagttctt cgccgcgcgc atccgcaagg aacgcgactg ggagcactac tggccggcgc
     2221 gcccgcccca ggcgcggctc attctcgacc gccgcgggct gcgccaggac tacgtgatct
     2281 gcggcgagat gtggtgtacg ccgctcgaga agctcgtgcc gcgggcgcgg gcgccggtcg
     2341 tgcagatcgc gccctgctag gcgccgttca gatggcaggc ggaaaggtgg tccgcggcga
     2401 ccttgcgcag ccgcggcgct tcctcgcggc agcgtggcag cgcgtgcgga cagcgcggat
     2461 ggaagtggca gcccgcaggc ggggacagcg gcgaagggat ctcgccggcg atcggcttga
     2521 actgggtttt gcgcgtatcg aggcgcggca cctgcttgag cagcgcctgg gtatacgggt
     2581 ggttcggccg cgcgaagatc tcctcggcgc tcgccgattc gacga
//
LOCUS       DASUNT010000329         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_746421, whole genome shotgun sequence.
ACCESSION   DASUNT010000329 DASUNT010000000
VERSION     DASUNT010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746421"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1303
                     /locus_tag="VFX94_06645"
     CDS             29..1303
                     /locus_tag="VFX94_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5767896.1"
                     /translation="MSHENQIRPSEGLRHVKYEIRGRLARRAQELERQGYEIVPLNIG
                     TPGAFGFRTPETMRLAMIENMAVAEGYCHQKGIFPAREAVVMQQQMRGVTGVSAEEVF
                     IGNGVSELIDLCLRALLNDGDEVLIPSPDYPLWSAAVTLNGGKAVYYPCKPENDFVPD
                     PEEIAALITRKTRAIVVINPNNPTGAVYPRAVCEAIAKLAETHKLVVFSDEIYDQMTY
                     DGVESIPMATLVRDTVCCSMSGLSKIYRACGYRVGWAAFSGNLRGASDYLSALELLSS
                     LRLCSNVPAQWAVQTALGGYQSIKELLAPGGRLYESRRAILEATSSSRFLHLAPPKGS
                     MYAFVRVKTDEIPDFDDQAFALDLLEQKHVLLAPGTSFNVPYRDHFRTTLLPEAALLK
                     KVFARIEELLASYAARTPSDASRVVQAASRFK"
     gene            1384..>2029
                     /locus_tag="VFX94_06650"
     CDS             1384..>2029
                     /locus_tag="VFX94_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767897.1"
                     /translation="MIVANAQRQASVRAAWSAAQRAGGQSLWNTPRVFTFTQFAEARL
                     GEQWSAANLPDRLLPAGAEWACLRDLRREGGGSGEARALLASVRTLRDWQIARSPAVL
                     GGSPEGNLLLEAMAALDRQSRDHGRKPLAEWIGGIEPLSGGLWSAGVGGLPSAQRAAL
                     QRLGAREVDIPAMSATVSVAAAEDDEHELASIADWCRAHLERDPARRLLVVDAKL"
BASE COUNT          334 a          690 c          680 g          325 t
ORIGIN      
        1 aggcccccgt cgcgctatcc ttcggcccat gtcgcacgaa aaccagattc gcccgagtga
       61 aggcctgcgc cacgtcaaat acgagatccg cggccgcctc gcccgtcgag cgcaggagct
      121 cgaacgccag ggctacgaaa tcgttccgct gaacatcggc accccgggcg cgttcggctt
      181 ccgcacgccc gagaccatgc gcctcgccat gatcgagaac atggccgtgg ccgaagggta
      241 ctgccaccag aagggcatct tcccggcgcg cgaagccgtg gtcatgcagc agcagatgcg
      301 cggcgtgacc ggcgtcagcg cggaagaggt gttcatcggc aatggcgtca gcgagctgat
      361 cgacctgtgc ctgcgcgcgc tgctcaatga cggcgacgag gtgctgattc cgagccccga
      421 ctacccgttg tggtcggcgg cggtcacgct gaacggcggc aaggccgtct actacccgtg
      481 caagccggag aacgatttcg tcccggaccc ggaagaaatc gccgcgctga tcacgcgcaa
      541 gacccgcgcc atcgtcgtca tcaacccgaa caacccgacc ggcgcggtgt atccgcgcgc
      601 ggtttgcgag gccatcgcca agctcgccga gacccacaag ctggtggtgt tcagcgacga
      661 gatctacgac cagatgacct acgacggcgt cgaatccatt ccgatggcga cgctggtgcg
      721 agacacggtg tgctgctcga tgtcgggcct gtcgaagatc taccgcgcct gcggttaccg
      781 cgtcggctgg gccgcgttct ccggcaacct gcgcggcgcc tcggactatc tatcggcgct
      841 cgagctgctg agctcgctgc gactgtgctc gaacgtgccg gcgcaatggg cggtgcagac
      901 cgcgctcggc gggtaccaga gcatcaagga attgctggcg ccgggcggac gcctgtacga
      961 atcgcgccgc gccatcctcg aggccacctc gtcgagccgc ttcctgcatc tcgcgccgcc
     1021 gaagggatcg atgtacgcct tcgttcgcgt gaagaccgac gagattcccg acttcgacga
     1081 ccaggccttc gcgctcgatc tgctcgagca gaagcacgtg ctgctggcgc cgggcaccag
     1141 cttcaatgtt ccgtaccgcg atcatttccg caccacgttg ctgccggaag cggcgctgct
     1201 caagaaggtg ttcgcgcgca tcgaagagct gctggcctcg tacgcggcgc gcacgcccag
     1261 cgacgccagc cgcgtggtgc aagcggccag ccgcttcaaa taggttgaag acctcagcgc
     1321 aagctattga acgataatag actttccgcg gccatttccg gggctttggc ctcgggagcg
     1381 acgttgatcg tcgccaacgc gcagcgccag gcctcggtgc gagcggcctg gtctgccgca
     1441 caacgtgccg gcggccagtc cctgtggaat acgccgcgcg tattcacttt cacgcaattc
     1501 gccgaagccc gcttgggcga gcagtggagc gccgccaatc ttcccgaccg cctgttaccc
     1561 gcgggagcgg agtgggcctg cctgcgcgac ctgcgccggg aaggcggtgg cagcggcgag
     1621 gcgcgtgcct tgctggcctc ggtgcgaacc ctgcgcgact ggcagatcgc ccgcagtccc
     1681 gccgtgctcg gcggctcgcc cgaaggcaac ctgctgctcg aggccatggc ggcgctcgat
     1741 cgtcagtcgc gcgaccatgg ccgcaagccg ctggcggagt ggataggcgg gatcgagccc
     1801 ttgtccggcg ggttgtggag cgcgggagtc ggtggcctgc cgtcggcgca acgcgccgcg
     1861 ctgcagcggc tgggcgcgcg cgaagtggac attccggcca tgtccgccac cgtgtccgtg
     1921 gcggcagccg aagacgatga gcacgagctg gcatcgatcg ccgactggtg ccgcgcgcat
     1981 ctcgagcggg atccggcgcg ccgcctgctg gtcgtggatg ccaaactgc
//
LOCUS       DASUNT010000330         7130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_659320, whole genome shotgun sequence.
ACCESSION   DASUNT010000330 DASUNT010000000
VERSION     DASUNT010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659320"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VFX94_06655"
     CDS             <1..558
                     /locus_tag="VFX94_06655"
                     /inference="COORDINATES: protein motif:HMM:NF018439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HEX5767898.1"
                     /translation="VNSLFDMEETSYPLVNTDYRVGFTYELQRGKLSARTRLFHQSSH
                     LGDELILQGNAPQRVNLSVEVVDLLLAWQWGNWRPYGGGMYIIHRDPDDLKQAGFQVG
                     LDYTGSARVLFGGRLVGGIDYRSFEENDWRGGMSVKVGLEYGPPRPERRGIKVLLEYY
                     DGAAPWGQFYNDVISYYGLGVQFDF"
     gene            575..1423
                     /locus_tag="VFX94_06660"
     CDS             575..1423
                     /locus_tag="VFX94_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEX5767899.1"
                     /translation="MAALLGFDVYSPSEIAERVSQVGVAKARLPIVPLVMLGMLAGAF
                     IALGSLYYVIVVADPALPHALQRLLGGTVFSLGLLLVVVAGAELFTGNNLLAMAWADG
                     KLGTREVLFNWLVVCGANLVGAVGVASMVYLAGHADAYAATYLKIAAAKTSLPLVQAF
                     FSGVMCNVLVCIAVWMALAGRSVVDKAVAIVFPISAFVAAGFEHCVANMYFLSLAAML
                     QAAAGEPVALSGIARNLVPVIAGNIVGGSVLVALVYYVIYRWPPRARGRSASGAPNAD
                     QMPTVR"
     gene            1461..1835
                     /locus_tag="VFX94_06665"
     CDS             1461..1835
                     /locus_tag="VFX94_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5767900.1"
                     /translation="MELVRKLACVALALAGLGGGAWADEPASGREVFDYRCRTCHGGT
                     RKADSPLGPSLAGIVGTRAGTQSSGVHSRALIESGTVWDRASLRRFLADPPREVPGNY
                     MPAVVVPPAELESLLDYLESFR"
     gene            1841..2983
                     /locus_tag="VFX94_06670"
     CDS             1841..2983
                     /locus_tag="VFX94_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5767901.1"
                     /translation="MSSDRAVLRQPQPYPETRLPPGVRPMRRPPALGRAVELAFLRWE
                     KRRLEPTERFWRDFGMHIVSATPGRIVARGAGAAPCIAIAERGKDNRFVGPAFRMSDD
                     TDLERYATEPGAAWVAPELIPGGGRGVELRDPAGRSVWLLQGQARVERLPLREALASS
                     TNTAHHTPRVNRMVRAPIEPAQVVKLGHLVLQTVDFARMADWYLRMLGLIPTDVQYLA
                     DGSPNLAFCRLDLGAQPADHHTVVLLGGIEEKYEHSAYEVLDLDALGQGQQVLRAQGH
                     RHMWGIGRHVLGSQLFDYWFDPDGFEHEHYTDGDVFTADFETQYSPLEFGALWAWGED
                     APASMKPKKNLRTLLKVLGLLRRKAITPQRLKLIGEALDAPARPWS"
     gene            2989..3945
                     /locus_tag="VFX94_06675"
     CDS             2989..3945
                     /locus_tag="VFX94_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5767902.1"
                     /translation="MARTLIRYALDGSPEWGVQFGELIAPLRVDAASTGDLLDRHWDK
                     IWSAAPAQATVRRESVRLLSPVTINQQFLCQGVNYRSHVRESGLRIEHFRFNTVFTKA
                     PSCITAADAPVACPAQVQLLDYEIELGLVLRTDVPAGTKVRPDELATWLAGVTIVNDL
                     SARDVQLPQGQFYKGKSFRGFGPVGPGLVLLTPAEWARWPELHMRLAVNGQARQDDYC
                     GEMIHAPAATLTELASVHDLNAGDLIATGTPAGCAARAPGKLAMFVLRHFVSEPAKWR
                     LFIRKGRTNPAYLRPGDRISASIRTDDGAIDLGEQTTVITTA"
     gene            3948..5819
                     /locus_tag="VFX94_06680"
     CDS             3948..5819
                     /locus_tag="VFX94_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX5767903.1"
                     /translation="MRAIATIADVLAIERQPPSLPPSTYEMIRAAASRHASAPALSFF
                     ARVEDHERPMTLSYESFFARITASANFFHALGIGKDDVVAFVLPNLPQTHLTIWGGEA
                     AGIVFAINPLLEPAAIAELLQAGEAKVLVTFADMSSRLEPLLGALPGLEHLVLVGSPE
                     QAPRIPGLAVHDFAEGTARQPSDRLVSARAIRPGDLSSFFCTGGTTGTPKIAMRTHAN
                     EVANAWSVGQMFGDAIGAGKTLFCGLPLFHVNAVLVTGLLPFSRGAQVLLGTPQGYRA
                     EGVVPRFWELVERHKINFFSGVPTLYAALLQQPIEGRDTGSLEYALCGAAPMPVELMR
                     NFEEKTGVRILEGYGLTEATCVSSCNPPLGERRLGSIGLRLPLQAMKPVVLDDSGRYV
                     RDCGHGEAGVLVVRGPNVFTGYKVPEHNKALWVDAGDGARWLNTGDLGRQDAEGYFYL
                     TGRKKELIIRGGHNIDPAALEEPLHRHPAVQMAAAVGRPDAHAGEVAVAYVQLKPGAS
                     ATEPDLMSFARDHIAERAAWPKAIRIIGAMPLTGVGKIFKPQLKQREIEDALNEALAQ
                     AGVQTRSLKVGNDAAHGIAIEVSLAEDADAVRARQVLGQYAFQFRLLPNCDRMPTVR"
     gene            5855..6718
                     /locus_tag="VFX94_06685"
     CDS             5855..6718
                     /locus_tag="VFX94_06685"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX5767904.1"
                     /translation="MNSPQTIGVIGAGTMGTGIVQVGALCGLSVVVMDIDEARVAKGR
                     AAVTGALEKLVKKEKLSAADRDAALGRLRGTTDYSALGTCDFIIEAATEDEPLKLKIL
                     RQMDAIAKNDAILATNTSSISITKLGAATRRPARFVGLHFFNPVPLMALVEVVRGRQT
                     DDAPVETAVALAKRLGKTPVVVKDSPGFVVNRLLCPMLNEAVLAYGEGIASAKDIDEA
                     MKLGCNHPIGPLALADLIGLDVVLAVMEVFHRDFKDPKYRPAPLLKEMVAAGKLGRKS
                     GRGFHTYEATQ"
     gene            6818..>7130
                     /locus_tag="VFX94_06690"
     CDS             6818..>7130
                     /locus_tag="VFX94_06690"
                     /inference="COORDINATES: protein motif:HMM:NF022783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HEX5767905.1"
                     /translation="MDLGQKLSRSVDISERITRVLQQRLQTATERFRSRVEAATKGLQ
                     PAAAWWAWPQYAVDCAQRAVIFWDTLRQRGNNYLEHVQQGQPPVLHFKYETVLDGRTL
                     ER"
BASE COUNT         1112 a         2453 c         2409 g         1156 t
ORIGIN      
        1 gtgaactctt tgttcgacat ggaggaaacg tcctatccgc tggtcaacac cgactaccgc
       61 gtcgggttca cgtacgaatt gcagcgcggg aagctctcgg cgcgcacgcg gctctttcac
      121 cagagctcgc accttggcga cgaattgatc ctccagggca atgctccgca acgcgtcaac
      181 ctgagcgtcg aggtcgtgga tctgctgctc gcctggcagt ggggtaactg gcggccctat
      241 ggcggaggga tgtacatcat ccaccgcgac cccgatgacc tcaagcaggc cggattccag
      301 gtcggcctgg actataccgg ctcggcgcgg gtcctcttcg gcggacgtct tgtcggcggc
      361 atcgactaca ggtcattcga ggagaacgac tggcgcgggg gcatgagcgt caaggtgggc
      421 ctggaatacg gcccgccgcg ccccgaacgc cgcggaatca aggtgctcct cgagtactac
      481 gacggcgccg cgccgtgggg ccagttctac aacgacgtca tctcgtatta cggcctcggc
      541 gtgcagttcg atttctgatc cgcgttgcag cgccatggcc gcactcctcg gcttcgacgt
      601 ctattcgccg agcgaaatcg ccgagcgcgt gtcgcaggtc ggggtggcga aggcgcgcct
      661 gcccatcgtt ccgctcgtca tgctcggcat gctcgccggc gcgttcatcg cgctcgggtc
      721 cttgtattac gtgatcgtgg tggcggaccc ggcgctgccg catgcgctcc agcgcctgct
      781 tggcggcacg gtgttttccc tcgggctcct gcttgtcgtc gtcgcgggcg cggagctctt
      841 caccggcaac aacctcctcg ccatggcgtg ggccgatggg aagctcggca cccgcgaggt
      901 gctcttcaac tggctcgtgg tctgtggcgc gaatctggtc ggcgccgtcg gcgtcgcgtc
      961 gatggtgtac ctcgccggcc acgccgacgc ctacgccgcc acgtatctca agatcgccgc
     1021 cgccaagacc tcgctgccgc tcgtgcaggc gttcttcagc ggcgtgatgt gcaacgtgct
     1081 cgtctgcatc gcggtgtgga tggcgctcgc cgggcgcagc gtcgtggaca aggcagtcgc
     1141 gatcgtgttc ccgatctcgg cgttcgtcgc cgccggcttc gagcactgcg tggccaacat
     1201 gtatttcctt tcgctcgccg cgatgctgca ggcggcggcg ggcgagccgg tagcgctttc
     1261 cggcatcgcg cgcaacctcg tgcccgtcat cgcgggcaac atcgtgggcg gtagcgtgct
     1321 cgtcgccctc gtgtactacg tgatctaccg ctggccgccg cgcgcgcgag ggcgcagcgc
     1381 atcgggcgca ccgaatgccg accaaatgcc gacggttcgc tgacgactcg gggaggcgcc
     1441 ggccgctagc ctcggcctct atggagctcg ttcgaaagct cgcgtgcgtg gcgctcgctc
     1501 tcgcaggcct gggaggcggc gcctgggcgg acgaacctgc gtccggccgc gaggttttcg
     1561 actaccgctg ccgcacttgc catggcggca cgcgcaaggc ggattctccg ctcggcccga
     1621 gcctcgccgg aatcgtgggc accagggcgg gcacgcaatc ctccggggtg cactcgcgcg
     1681 ccttgatcga gtccgggacc gtatgggatc gcgcatcgtt gcgccgcttc ctcgccgatc
     1741 caccgcgcga ggttccgggc aactacatgc ctgcagtggt cgtaccgccg gcggagctgg
     1801 aaagcctgct cgactatctc gagtcatttc gctgagccgc atgagcagcg accgcgccgt
     1861 gctgcgccag ccgcaaccgt atccggaaac ccggctgccg ccgggcgtgc gcccgatgcg
     1921 gcgcccgccc gcgctcgggc gggccgtgga gctcgccttc ctgcgctggg agaagcgccg
     1981 cctggagccg accgagcgct tctggcgcga cttcggcatg cacatcgtca gcgcaacgcc
     2041 cgggcgcatc gtggcccggg gtgcgggtgc ggcgccctgc atagcgatcg cggagcgcgg
     2101 aaaagacaat cgcttcgtcg gcccggcgtt tcgcatgtcc gacgacaccg acctcgagcg
     2161 ctacgccacc gagccgggcg cggcctgggt cgcgcccgag ctcatccccg ggggcgggcg
     2221 cggcgtcgag cttcgggatc cggccggccg cagcgtttgg ctgctgcagg gccaagcccg
     2281 ggtcgagcga ctgccgttgc gcgaggcgct ggcctcgagc accaataccg cgcatcacac
     2341 cccgcgcgtg aaccgcatgg tgcgcgcgcc gatcgagccg gcgcaggtcg tgaagctcgg
     2401 gcacctggtc ctgcagacgg tcgatttcgc gcgcatggcc gactggtatt tgcggatgct
     2461 cggtctcatc ccgaccgacg tgcagtatct cgccgacgga agtccgaacc ttgccttctg
     2521 ccgcctcgat cttggcgctc agcccgcgga ccatcacacc gtcgtgctcc tgggcggcat
     2581 cgaggagaag tacgagcaca gcgcctacga agtcctcgac ctggatgcgc tcggtcaggg
     2641 tcagcaggtg ttgcgcgcgc aggggcaccg gcacatgtgg gggatcgggc ggcacgtcct
     2701 tggcagccag cttttcgact actggttcga ccccgacggc ttcgagcacg agcactacac
     2761 cgacggagac gtgttcaccg cggatttcga gactcaatac tcgccgctcg agttcggcgc
     2821 cctctgggcc tggggcgaag acgctccggc ctcgatgaag ccgaaaaaga accttcgcac
     2881 cttgctcaag gtgctcggcc tcttgcggcg caaggccatc acgccgcagc ggctcaagct
     2941 catcggcgaa gcgctcgacg cgcccgcgcg cccctggagc tgaacggcat ggctcgcacc
     3001 ctcatccgct acgccctcga cggctcgccc gaatggggcg tgcagttcgg agagctcatc
     3061 gcgccgctgc gcgtggacgc agcgagcacc ggagatctgc tcgatcggca ctgggacaag
     3121 atctggtcgg ctgcgccggc gcaggcgacc gtccggcgcg agagcgtgcg gctcctttct
     3181 ccggtgacca tcaaccagca gttcctctgc cagggcgtga attaccgcag ccacgtgcgc
     3241 gagtccggcc tgcgcatcga gcactttcgc ttcaacacgg tcttcaccaa ggcgccctcg
     3301 tgcatcacgg cggccgatgc gccggtcgcc tgccccgcgc aggtgcagct tctcgactac
     3361 gagatcgagc tcggcctcgt gctgcggacc gatgtgcccg cgggaacgaa ggttcgcccg
     3421 gatgagctcg cgacctggct cgcgggcgtg acgatcgtca acgatctctc ggcgcgcgac
     3481 gtgcagctgc cgcaaggcca gttctacaag ggcaagagct tccgcggctt cggcccggtc
     3541 gggccgggac tcgtgctgct cacgcccgcg gagtgggcgc gctggccgga gctgcacatg
     3601 cggctcgccg tcaacgggca ggcgcggcag gacgactact gcggcgagat gatccatgcg
     3661 ccggccgcga cgctgaccga gctcgcctcg gtgcacgacc tgaacgccgg cgacctgatc
     3721 gcgaccggca cgccggcggg ctgcgcggcg cgcgcgcccg ggaagctggc gatgttcgtt
     3781 ctgcgccact tcgtgagcga acccgccaag tggcgcctct tcatccgcaa ggggcgcacc
     3841 aacccggcgt acctgcgacc cggcgaccgc atcagcgcct cgatccgcac tgatgacggg
     3901 gcgatcgacc tgggcgagca gaccacggtc attaccacgg cgtgagcatg agagcgattg
     3961 cgaccattgc cgatgtcctc gccatcgaac ggcagcctcc ctcgctgccg ccgagcactt
     4021 acgaaatgat tcgcgcagcc gcctcgcggc acgccagcgc gccggcgctg tcgttcttcg
     4081 cgcgcgtcga ggatcacgag cgaccgatga ccttgagcta cgagtcgttc ttcgcccgca
     4141 tcacggcgag cgccaacttc tttcacgcgc tcggtatcgg caaggacgat gtcgtggcct
     4201 tcgtgctgcc gaaccttccg caaacgcatc tcacgatctg gggcggggaa gcggccggaa
     4261 tcgtgttcgc catcaatccg ctgctcgagc cggcggcaat cgccgagctg ctccaggcgg
     4321 gcgaggcgaa ggtgctcgtg acgttcgcgg acatgtcttc gaggctcgag ccgctccttg
     4381 gcgcgctccc gggcctcgag catctcgtcc tggttggctc cccggagcag gcgccgcgca
     4441 tcccggggct cgcggtgcac gacttcgccg agggcaccgc gcgccagccg agcgaccgcc
     4501 tcgtcagcgc ccgcgcgatc cggcccggcg atctttcctc gttcttctgc accggcggca
     4561 cgaccggcac gccgaagatc gccatgcgca cccacgcgaa cgaggtggcc aacgcctgga
     4621 gcgtgggcca gatgttcggc gacgccatcg gcgccggcaa gacgctcttc tgcggcctgc
     4681 cgctcttcca cgtaaacgca gtgctcgtca cgggcctgct gccgttctcg cgcggcgcgc
     4741 aggtgctgct cggcacgccg cagggctacc gcgccgaggg cgtcgtgccg cgcttctggg
     4801 agctcgtcga gcggcacaag atcaatttct tcagcggcgt tccgacgctg tacgccgcgc
     4861 tccttcagca gccgatcgaa ggccgcgaca cagggtcgct ggaatacgcg ctgtgcgggg
     4921 cggcgccgat gccggtcgaa ctgatgcgca atttcgagga aaagaccggc gtcaggattc
     4981 tcgagggata cggcctcacc gaagccacct gcgtgagcag ctgcaatccg ccgctcggcg
     5041 agcggcgcct cggctcgatc gggctgcgcc tgccgctgca ggcgatgaag cccgtggtgc
     5101 tcgacgactc gggccggtac gtgcgtgact gcggccacgg cgaagcgggc gtcctcgtgg
     5161 tccggggtcc gaacgtgttc acgggctaca aggtgcccga gcacaacaag gcactctggg
     5221 tcgatgccgg cgatggcgcg cgatggctga acaccggcga cctcggccgg caggacgccg
     5281 aaggctactt ctacctcacg ggcagaaaga aggagctcat catccgcggc ggccacaaca
     5341 tcgatccggc ggcgctcgag gagccgctgc atcgacaccc cgccgtgcag atggccgcgg
     5401 cagtcggcag acccgacgcg catgcgggcg aggtggcggt ggcctacgtg cagctcaagc
     5461 cgggcgcgag cgccaccgag cccgatctca tgtccttcgc cagggatcac atcgccgagc
     5521 gcgccgcatg gcccaaggcg atccgcatca tcggcgcgat gccgctgacc ggcgtcggga
     5581 agatcttcaa gccgcagctc aagcagcggg aaatcgaaga cgcgctgaac gaggcgctcg
     5641 cgcaggcagg cgtgcagacc cgctcgctca aggtcggcaa cgatgcggct cacggaattg
     5701 cgattgaggt gtcgctcgcc gaagacgccg acgccgtgcg cgcgcgccag gtgctcggtc
     5761 agtacgcctt ccagttccgg ctgttaccca attgcgaccg aatgccaacc gttcgctgac
     5821 gacttggcgg acggcggggg ctaaggtcgc tgtcatgaac tccccgcaaa cgatcggcgt
     5881 catcggcgcc ggaaccatgg gcaccggcat tgtgcaggtc ggcgcgctct gcggtctttc
     5941 ggtcgtggta atggacatcg acgaggcgcg cgtcgcgaaa gggcgcgccg ccgtgacggg
     6001 cgcgctcgag aagcttgtca agaaggagaa gctctccgcc gccgaccggg acgcggcact
     6061 cggccgcctg cgcggcacga ccgactacag tgcgctcggg acttgcgact tcatcatcga
     6121 agcggcaacg gaagacgagc cgctgaagct caagatcctg cggcagatgg acgcgatcgc
     6181 gaagaacgac gccatcctcg caaccaacac ctcgtcgatc tcgatcacca agctcggcgc
     6241 ggcgacgagg cggccggcgc gcttcgtcgg cctgcatttc ttcaacccgg tgccgctcat
     6301 ggcgctcgtc gaggtggtgc gcggccggca gaccgacgat gcgcccgtcg agacggcggt
     6361 cgcgctcgcg aagcgcctcg gcaagacgcc ggtcgtggtg aaggactctc cagggttcgt
     6421 cgtgaaccgc ctgctctgcc cgatgctgaa cgaggcggtg ctcgcgtatg gcgaaggcat
     6481 cgcctccgcg aaggatatcg acgaggcgat gaagcttggc tgcaaccacc cgatcggccc
     6541 gctcgcgctc gccgacctga tcggcctgga cgtcgtgctc gcggtgatgg aggtgttcca
     6601 ccgcgacttc aaggatccca agtaccggcc ggcgccgctt ctgaaggaga tggtggcggc
     6661 cggcaagctc ggccgcaagt ccggccgcgg attccatacc tatgaggcca cccaatgaac
     6721 cgtgcatacc gcaaactgat cctcgcgctg gcggtcgccc ggattacggg gcggaactgg
     6781 aattgtgcga agtgctactc gtgagcgcgc gttcgacatg gacctaggcc aaaagctttc
     6841 ccgcagcgtc gacatttccg aacgcatcac ccgcgtgctg caacagcgcc tgcagaccgc
     6901 gaccgagcgg tttcgcagcc gcgtggaggc ggcaacgaag ggcttgcagc ccgcggccgc
     6961 gtggtgggcg tggccgcagt acgcggtcga ctgcgcgcaa cgcgcggtga tcttctggga
     7021 cacgctgcgc cagcgcggca ataactatct cgagcatgtg cagcagggcc agccgccggt
     7081 cctgcacttc aagtacgaga cggtgctcga cgggcgcacc ctcgagcggc
//
LOCUS       DASUNT010000331         2859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1010279, whole genome shotgun sequence.
ACCESSION   DASUNT010000331 DASUNT010000000
VERSION     DASUNT010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010279"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VFX94_06695"
     CDS             <1..1126
                     /locus_tag="VFX94_06695"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5767906.1"
                     /translation="TLIGTRFDRRWVKYVFITLDIAVVSALVATQPLYPSAAGLPAVM
                     TFRAPVFPFYFVILGVAAFTFSPAMVMWTGVAGALGWLAAFWHAASAVEGVRNWSEIP
                     PTPTAEQIMEVVLDPAFGGLSARIQEAVLLVVVAFLVAVVMWRARTTLKRQLEAERDH
                     ATLSGIFGRFVPQAIVNAMVAGRGALAPVEREATVLFADIAGFTEMTERAGAARTVEI
                     LNAYFDEITRIIGAHNGIVTQFQGDAVLATFNVPVEDVGHAVNAFRAARAILAAVAER
                     EFAGERVRVRIGINTGSLVAGNVGGGGRQSYTVHGDTVNLAARLEALCKEHGTSLLLS
                     AATAKALPGAKLLAVGTISVRGLSEPVAVYSTPPGIAAAA"
     gene            1461..1919
                     /locus_tag="VFX94_06700"
     CDS             1461..1919
                     /locus_tag="VFX94_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX5767907.1"
                     /translation="MHTPAQIAKHPIHPMLVPIPIGLWIFSLVCDLVFVFGGGAETWR
                     TVAFYTMAGGIVGALIAAVPGFIDMLSLRGQLKRIALMHMTLNLVIVAMYAINLGWRI
                     GDAYGSGPVWLSLVAVLLLGVSGWLGGKMVYEHGVAVHTAREGERGQLQA"
     gene            complement(1940..>2859)
                     /locus_tag="VFX94_06705"
     CDS             complement(1940..>2859)
                     /locus_tag="VFX94_06705"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5767908.1"
                     /translation="VLLICIALAAAVTLGYLPARGFWLFLALALLHAAFLAPMTVLAD
                     ALALASSKRERFEYGWVRGTGSAAFIAGLLASGQLVGAHGLDAIIWSQALLLAMSALA
                     AARVPEIANERAHAPPVSALAPLRIPLFRNMVLVAAMILGSHAMHDAFAMIRWSAAGI
                     GPSAASVLWSISVASEVLVFFVIGPWLVTWLTPRGAIVLAGLAGVLRWAVMAQTTDVF
                     ALALVQPLHGLTFALLHLACMRVIGLVVPHGVEGTAQALYATVGIGAATAVLTLASGV
                     LYARLGASGFWIMAALCAAALPLALRLRT"
BASE COUNT          491 a          945 c          972 g          451 t
ORIGIN      
        1 cacgctcatc ggcactcgct tcgacaggcg ctgggtgaag tacgtgttca ttacgctcga
       61 catcgccgtc gtttcggcac tcgtggcgac gcagcccctc tacccgtccg ccgccggcct
      121 gcctgcggtc atgacgttcc gcgcgccggt gttcccgttc tattttgtaa tcctcggcgt
      181 ggccgcattc acgttctcgc ccgcgatggt gatgtggacc ggcgtggcgg gtgcgctcgg
      241 atggctcgcg gcgttctggc atgcggcaag cgcggtcgag ggcgtgcgca actggagcga
      301 gataccgccc accccgacgg ccgagcagat tatggaggtg gtgctcgatc ccgcattcgg
      361 cgggctcagc gcccggattc aggaggcagt cctgctcgta gttgtggctt tcctcgttgc
      421 cgtggtcatg tggcgcgcgc gcaccacgct gaaacgtcaa ctggaagcgg aacgcgacca
      481 cgccaccctc tccggcattt tcggccgctt cgtgccgcag gcgatcgtga acgccatggt
      541 cgccgggcgc ggggctctgg cgccggtcga gcgcgaggca accgtgctat tcgccgacat
      601 cgccggcttc accgaaatga ccgagcgagc cggcgcggcg cgcaccgtgg agatcctgaa
      661 cgcctacttc gacgagataa cgcggatcat cggggcccat aacggcatcg tcacgcagtt
      721 ccagggcgat gccgtgctcg ccacgttcaa cgtaccggtc gaggatgtcg gccacgcggt
      781 caacgccttc agagctgccc gggcgattct ggctgccgtt gcggagcgcg aattcgccgg
      841 agagcgtgtt cgggtgcgca taggcatcaa tacgggttcg ctggtcgccg gcaatgtcgg
      901 cggcggcggt cgccagagct atacagtgca tggggacacg gtgaacctcg ccgcccggct
      961 cgaagcgctt tgcaaggagc atggcacctc gctccttctt tcggctgcta cggccaaggc
     1021 gttgccaggc gcgaagctcc tcgcggtggg gacgatctca gtgcgcggcc tgtccgagcc
     1081 ggtggccgtc tactcaaccc cgccaggcat agcggccgcc gcatgaccat agtggagtgc
     1141 ctccaacagc attcgcgtcg ctcaaaggac ctcgttgaag aggtagagag cgctatcttg
     1201 cgcgcggtgc agcaggccgc gcctcttcca gcgggcggca tcgagtgggg gtccttgtgc
     1261 cgcttgatgg accagatggc gacgccgagc actgcgacgg cgaacaaaag cacggcgatg
     1321 atccaaccca tggccgccag atactaaggg cctaggccgg cgctgtcccg gcccgggaag
     1381 ctcatggcct gggccgggcc cgatgattgc aacgcggctg ggatctgtaa ccccgatggc
     1441 atacatacgg aggccctttc atgcacaccc cggcgcagat agccaagcac ccgatccacc
     1501 cgatgctcgt tcccataccg atcggcctgt ggatcttttc cctggtctgc gacctggtat
     1561 tcgtcttcgg cggcggcgcc gagacgtggc gcacggtggc gttctatacg atggccggcg
     1621 gcatcgtggg cgcgctcatc gccgccgtgc ccggctttat cgacatgctg tcgctgcgcg
     1681 ggcagctcaa gcgcatcgcg ctcatgcaca tgacgctcaa tctggtgatc gtggcgatgt
     1741 acgcgatcaa cctcggatgg cgtatcggcg atgcgtacgg gtcgggcccc gtgtggctat
     1801 cgctggttgc cgtgctgcta ctcggcgtgt cgggctggct gggcggcaag atggtgtacg
     1861 agcacggcgt cgcggtccat accgcccgcg agggcgagcg cggtcaattg caagcttgaa
     1921 ggcgtactta acgcgcatgt caggttcgca accgcagggc gagcggcaac gcggcggcgc
     1981 agagcgcagc catgatccag aacccggagg ctccgaggcg cgcgtacaaa acgcctgatg
     2041 ctaatgtcag cacggcggtc gccgcgccga tacccacggt ggcgtagagc gcctgcgcgg
     2101 tcccttccac gccgtgcggc acgacaagcc ctatgacgcg catgcaggcg agatggagca
     2161 acgcgaaggt gaggccgtga agcggctgca cgagcgcgag cgcaaagaca tcggtcgttt
     2221 gcgccatcac cgcccagcgc agcacgccgg ccagcccggc cagcacgatg gcgccgcgcg
     2281 gcgtgagcca tgtcacgagc caaggcccga tcacaaagaa gaccagcacc tcggaggcga
     2341 cggagatcga ccacagcacg cttgccgccg agggaccgat cccggccgcg ctccagcgga
     2401 tcatggcaaa cgcatcgtgc atggcgtggc tgccgaggat catggccgcg accaacacca
     2461 tgttgcggaa gagcggaatg cgcagcggcg cgagcgcgct caccggcggc gcgtgcgcgc
     2521 gttcgttcgc gatctcgggc acacgcgcgg ccgcgagcgc gctcatggcg agcaggagcg
     2581 cctgcgacca gatgatcgcg tcgaggccgt gggcgcccac gagctgcccc gaggcgagca
     2641 gccccgcgat gaaagcggcc gaccccgttc cccgcaccca gccgtactcg aagcgctccc
     2701 gcttggacga agcgagcgcg agcgcatcgg cgagcacggt catcggcgcc agaaatgctg
     2761 cgtggagcag cgcgagcgcg agaaacagcc aaaagccgcg cgccggcaga taaccgagcg
     2821 tcaccgcggc agcgagcgcg atgcagatca ggagcacca
//
LOCUS       DASUNT010000332         3063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_922683, whole genome shotgun sequence.
ACCESSION   DASUNT010000332 DASUNT010000000
VERSION     DASUNT010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922683"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1688)
                     /gene="napA"
                     /locus_tag="VFX94_06710"
     CDS             complement(<1..1688)
                     /gene="napA"
                     /locus_tag="VFX94_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HEX5767909.1"
                     /translation="MSLSRRDFLKAHAVAAAASVSGIPVKTLAADPAKESDIKWDKAA
                     CRFCGTGCSVLVGTQSGRVVATQGDPESEVNRGLNCVKGYFLSKIMYGADRLKAPLLR
                     KKNGVYDKAGEFTPVSWKEAFDVMATKWKEALKAGGPTSVGMFGSGQWTIWEGYAAAK
                     LMKAGFRSNNLDPNARHCMASAVAGFMRTYGIDEPAGCYDDLEHADAFVLWGSNMAEM
                     HPVLWSRITDRRLMNPHVRVAVLSTYQHRGHELADLHMIFTPQTDLAILNFIAHHIIS
                     TGRVNQAFVSRHVNFALGNADIGYGLRPEDEREKRAKNAGNAEAMKPISLEEYAKFVS
                     EYTVEKVSQLSGVPARLLLELAELYADPKRKVVSLWTMGFNQHTRGTWVNQLCHNVHL
                     LTGKIAEPGNHPFSLTGQPSACGTAREVGTFSHRLPADMVVANPKHREMTEKIWQLPA
                     GTIPEKPGYHAVLQNRMLKDGKLNAYWVQCTNNMQTAANLMQETWPGYRNPKNFVVVS
                     DPYPTVTAMAADLILPTAMWVEKEGAYGNTERRTQFWRQQVKAPGEAKSDVWQVV"
     gene            complement(1685..>3063)
                     /locus_tag="VFX94_06715"
     CDS             complement(1685..>3063)
                     /locus_tag="VFX94_06715"
                     /inference="COORDINATES: protein motif:HMM:NF014398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HEX5767910.1"
                     /translation="CPTTTFQVHKALRIGLAAALAACVTLLLAQAAITDDVDDPAAWG
                     KVFPLHYELYKKTVDMERTKYGGSEAVPQSPTPTDPRTVVSRSKVDEDIGLRTMWQGY
                     AFAVDFREERGHAYMLEDQRLTRRQQAAAQPGACLNCHASSYVAFKKAGGGDLTKGFE
                     KVNALPYAEAVKLVQHPVSCIDCHDPNTMALRITRPAFIEGMRALKASEGIQNYDVNK
                     QAGPEEMRAFVCGQCHVEYYFRGAEKRLVFPWAKGLKVENIQAYYDEIKFRDFTHRQT
                     GAPALKAQHPEFELWSQGIHARAGVKCADCHMPKIQYKDATLSDHWVRSPILNIENAC
                     LGCHRKREPALSAIEMRSRVEQIQDRHWSLREHAMTALLGLIGDLAKAKDAGRSDAEL
                     ATARYLQRRAQFYLDFVEAENSTGFHAPQEAARILGESLNYSRQGQLALRDKDFKPTL
                     PVADIPNK"
BASE COUNT          447 a         1000 c         1023 g          593 t
ORIGIN      
        1 accacctgcc agacatcgga tttggcctcc cccggcgcct tgacctgctg gcgccagaac
       61 tgggttcgcc gctcggtgtt gccgtaggcg ccttccttct cgacccacat cgcggtaggc
      121 aggatgaggt cggcggccat cgcggtcacg gtcggatacg ggtcgctcac caccacgaag
      181 ttcttcgggt tgcggtagcc gggccaggtt tcctgcatca ggttggccgc ggtctgcatg
      241 ttgttcgtgc attgcaccca gtaggcgttc agcttgccgt ccttcagcat ccggttctgc
      301 aacacggcgt ggtagccggg cttctccggg atcgtgcccg cgggcagctg ccagatcttc
      361 tccgtcatct cgcggtgctt cgggttcgcc accaccatgt cggcgggcag gcgatgcgag
      421 aacgtgccga cctcgcgcgc ggtgccgcac gccgagggct ggccggtgag cgagaacggg
      481 tggttgcccg gctcggcgat cttgcctgtg agcagatgca cgttgtggca gagctggttt
      541 acccacgtgc cgcgcgtgtg ctgattgaag cccatggtcc agagcgacac caccttgcgc
      601 ttcgggtcgg catacagctc cgcgagctcg aggagcagcc gcgcgggcac gcccgaaagc
      661 tgcgatacct tttcgacggt gtactcggaa acgaacttgg cgtattcctc gagcgagata
      721 ggcttcatcg cctcggcatt gccggcgttc ttcgcccgct tctcgcgctc gtcctcgggg
      781 cgcaggccgt agccgatgtc cgcgttgccg agcgcgaagt tgacgtgcct ggagacgaaa
      841 gcctggttaa ctcggccggt cgagatgatg tggtgcgcga tgaagttgag gatcgcgagg
      901 tcggtctgcg gcgtgaagat catgtgcagg tcggcgagct cgtggccgcg gtgctggtag
      961 gtcgagagca cggccaccct gacgtgcggg ttcatcagcc gccggtccgt gatgcgcgac
     1021 cagagcaccg ggtgcatctc cgccatgttc gagccccaga gcacgaatgc gtcggcgtgc
     1081 tcgaggtcgt cgtagcagcc cgccggctcg tcgatgccat acgtgcgcat gaatcccgcg
     1141 accgcggagg ccatgcaatg gcgcgcgttc ggatccaggt tgttcgagcg gaatcccgcc
     1201 ttcatgagct tggccgcggc gtagccctcc cagatcgtcc actgccccga gccgaacatg
     1261 ccgacgctgg tggggccgcc ggccttgagc gcctccttcc acttcgtcgc catgacgtcg
     1321 aaggcttcct tccacgacac cggcgtgaac tcgcccgcct tgtcgtagac accgttcttc
     1381 ttgcgcagga gcggcgcctt gaggcgatcg gcgccgtaca tgatcttgga gaggaaataa
     1441 cccttgacgc agttgaggcc ccggttcacc tccgactcgg gatcgccctg cgtcgccacc
     1501 acgcggccgc tctgcgtgcc gaccagcacg ctgcagcccg tgccgcagaa gcggcaggcc
     1561 gccttgtccc atttgatatc gctttccttc gcgggatccg ccgcaagcgt ctttacggga
     1621 atgcctgaaa cggaggccgc cgcggccacc gcgtgcgctt tgaggaaatc gcgtcgactg
     1681 aggctcattt gtttggaata tccgcgaccg gcaaggttgg cttgaaatcc ttgtcgcgca
     1741 gggcgagctg cccctggcgc gagtagttga gcgattcgcc caggatgcgc gccgcttcct
     1801 gcggggcgtg gaagccggtg gagttctccg cctcgacgaa gtcgagatag aactgcgcgc
     1861 ggcgctgcag gtagcgcgcg gtggcgagct cggcgtcgct ccgccccgca tctttcgcct
     1921 tggcgaggtc gccgatcagg ccgagcagcg cggtcatggc gtgctcgcgc agcgaccagt
     1981 gccggtcctg gatctgctcg acgcgcgagc gcatctcgat cgcgctaagc gcgggctcgc
     2041 gcttgcgatg gcagccgagg cacgcgttct cgatgttgag aatcgggctt cgcacccagt
     2101 ggtcgctcag cgtcgcgtcc ttgtactgga tcttcggcat atggcagtcg gcgcacttca
     2161 cgccggcgcg cgcgtggatg ccctggctcc atagctcgaa ctccgggtgc tgcgccttga
     2221 gcgccggcgc gccggtctgc cggtgggtga agtcgcgaaa cttgatctcg tcgtagtagg
     2281 cctggatgtt ctcgaccttg aggcccttgg cccaggggaa cacgaggcgc ttctcggcgc
     2341 ccctgaagta gtactcgacg tggcactggc cgcacacgaa ggcgcgcatc tcctcggggc
     2401 ctgcctgctt gttgacgtcg tagttctgga tgccttcgga cgccttcaat gcgcgcatcc
     2461 cctcgatgaa cgccggccgg gtgatgcgta aggccatcgt gttagggtcg tggcagtcga
     2521 tgcacgacac cgggtgctgc acgagcttca ccgcctcggc gtacggcagc gcgttcacct
     2581 tctcgaagcc cttcgtcaga tcgccgccgc ccgccttctt gaacgcgacg tagctcgacg
     2641 catggcagtt cagacacgcg cccggttgcg ccgcggcctg ctggcgccgc gtgagccgct
     2701 ggtcttcgag catgtaggca tggccgcgct cctcgcggaa gtcgacggcg aaggcgtagc
     2761 cctgccacat cgtcctcagg ccgatgtcct cgtccacctt gctgcgcgag acgacggtac
     2821 gcggatcggt gggcgtgggg ctctggggca ccgcctcgct gccgccgtac ttggtgcgct
     2881 ccatgtccac cgtcttcttg tagagctcgt agtggagcgg gaagaccttg ccccaggcgg
     2941 cgggatcgtc cacatcgtcc gtgatcgccg cctgcgccag cagcagcgtt acgcaagccg
     3001 ccagcgcggc ggcgaggccg atgcgaaggg ctttatgcac ctggaacgtt gtagtgggac
     3061 acc
//
LOCUS       DASUNT010000333         2390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_572245, whole genome shotgun sequence.
ACCESSION   DASUNT010000333 DASUNT010000000
VERSION     DASUNT010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572245"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFX94_06720"
     CDS             <1..246
                     /locus_tag="VFX94_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007742682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX5767911.1"
                     /translation="AFLECFDALYTSYLAKASFEGVEQRTASLLPALLLARVDGKSPV
                     EYLSEPQRQQVRQFARPLVGRASSLAEIRSLWQRSST"
     gene            225..1469
                     /locus_tag="VFX94_06725"
     CDS             225..1469
                     /locus_tag="VFX94_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX5767912.1"
                     /translation="MAKILDVKGRQVWDSRGRATVEAEVIVGRGTTPLAMGRAIAPAG
                     ASTGAGEAKTVEPRKAVHNIQTRIRDGLRALSVSDQAKLDQRLIELDGTADKSRLGAN
                     AMVAVSLACAHADAAALKMPLWKKFSGEKRVALPVPQIQIFGGGAHARGRCDLQDYMI
                     ICIGAGSFAEALEWTSQVYAAAGKRLAKRGALQGVADEGGYWPAFKTNEEGLAELVGA
                     ISDAGLEPGADVAIALDIAASQLYRGGSYHLALEQRSLSAEQLHAMLLRWIERYPIVS
                     IEDPFAETDHAAMQAFTKAAGIQVVGDDYFVTRAEKVKSANGACNTVLLKPNQVGTLT
                     ETLACWEAARAAGYRAIVSARSGETEDVSIVHLAVGWGVPQLKVGSFSRSERMAKWNE
                     ALRIEEALGAKALPFPARKLFK"
     gene            1577..>2390
                     /gene="kaiC"
                     /locus_tag="VFX94_06730"
     CDS             1577..>2390
                     /gene="kaiC"
                     /locus_tag="VFX94_06730"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HEX5767913.1"
                     /translation="MKMERPAQAHSLPKARTGIPGLDEITEGGLPRGRPTLICGGPGC
                     GKTLLAAEFLIRGATEFDEPGALITFEETGEELAQNVRSLGFDLNALAEQNKIAIDYI
                     RVERSEIAETGEYDLEGLFLRLGLAIDSVGAKRIVLDTIESLFAGFENQAVLRSELRR
                     LFRFLKDRGVTAVITGERGDQSYTRQGLEEYVSDCVILLDHRVIEQVSTRRLRIVKYR
                     GTTHGTNEYPFLIDEDGITVLPITAMSLQHEVSDERISSGIPQLDTMLTGEGY"
BASE COUNT          414 a          810 c          793 g          373 t
ORIGIN      
        1 gccttcctcg aatgcttcga tgcgctgtat acctcgtatc tggcaaaagc atccttcgaa
       61 ggcgtggagc agcgaacggc ttccctgctg ccggcgctgc tgctcgcccg cgtcgatgga
      121 aagtcgccgg tcgaatacct gagcgagcct caacgccagc aggtgcgtca attcgcccgt
      181 cccctcgtgg ggcgcgcgtc gtccctcgcc gaaatcagga gcctgtggca aagatcctcg
      241 acgtaaaggg ccgccaggtg tgggactcgc gcggccgggc cacggtcgag gccgaggtga
      301 tcgttgggcg cggcaccacg ccgctcgcca tggggcgggc gatcgcgccg gccggcgcct
      361 cgaccggcgc cggcgaggcg aagacagtgg agccgcgcaa ggcggtgcac aacatccaga
      421 cgcgcatccg cgacggcctg cgcgcgctca gcgtctcgga ccaggcgaag ctcgaccagc
      481 gcctcatcga gctcgacggc acggcggaca agagccgcct cggcgccaat gccatggtcg
      541 cggtttccct ggcttgcgcg catgcggatg cagccgccct aaaaatgccg ttgtggaaga
      601 agttctctgg agaaaagcgg gtagcgctgc ccgtgccgca gatccagatc ttcggcggcg
      661 gcgcgcacgc gcgcgggcgc tgcgacctgc aggactacat gatcatctgc atcggcgcgg
      721 gcagcttcgc cgaagcgctg gaatggactt cgcaggtgta tgccgccgcc ggcaagcgcc
      781 tggcgaagcg cggcgcgctg cagggcgtcg ccgacgaggg cggctactgg ccggcgttca
      841 agaccaacga ggagggcctc gccgagctgg tgggcgcgat ctcggatgcg ggcctggagc
      901 ccggcgccga cgtggcgatc gccctcgaca tcgccgccag ccagctctac cgcggcggca
      961 gctaccacct cgccctcgag cagcgctcgc tctcggccga gcagctgcac gccatgctcc
     1021 tgcgctggat cgagcgttat ccgatcgtct cgatcgagga cccttttgca gaaacggacc
     1081 acgctgccat gcaggcgttt accaaggcgg ccggcatcca ggtggtcggc gatgactact
     1141 tcgtaacgcg tgcggagaaa gtcaaaagcg ccaacggcgc ctgcaacacc gtcctactga
     1201 aacccaatca ggtcggcacg ctcaccgaga ccctcgcctg ctgggaggcc gcgcgcgcgg
     1261 ccggctaccg cgccatcgtc tcggcgcgct cgggggaaac ggaggacgtt tccatcgtcc
     1321 acctggcggt gggctggggg gtgccgcagc tcaaggtcgg cagcttctcg cgctcggagc
     1381 gcatggcgaa atggaacgag gcgctgcgca tcgaggaggc cctcggcgcc aaggcgctcc
     1441 ccttccccgc ccgcaagctg ttcaagtagt cgaaaccgtg acgttgcggt tttgcgacac
     1501 gacacatctg tcgggtgttt cactgtcatc gggtttagaa tccgcactct cctttgccaa
     1561 aaaactcacc gcaaaaatga agatggaacg cccggcgcag gcgcattccc ttccgaaagc
     1621 gcgcactggt atcccagggc tcgatgagat caccgaaggc ggcctgcctc gcggccggcc
     1681 cacgctcatc tgcggcggcc cgggttgcgg caaaacgctg ctcgccgccg agttcctgat
     1741 ccgcggcgcg accgagttcg acgagcccgg cgccttgatc accttcgagg agaccggcga
     1801 agagctcgcg cagaacgtgc gctcgctcgg cttcgacctc aacgcgctgg cggaacagaa
     1861 caagatcgcg atcgattaca tccgggtcga gcgctccgag atcgcggaga ccggcgagta
     1921 cgacctcgag ggcctgttcc tgcggctcgg gctggcgatt gactcggtcg gggccaagcg
     1981 catcgtgctc gacaccatcg aatctctgtt cgccggcttc gagaatcagg cggtgctgcg
     2041 ctcggagctt cggcgcctgt tccgcttcct caaggaccgc ggcgtcaccg cggtcatcac
     2101 cggcgagcgc ggcgaccagt cctacacgcg ccagggcctg gaggagtacg tctccgactg
     2161 cgtgatcctg ctcgaccacc gggtgatcga gcaggtgtcc acgcggcggc tgcgcatcgt
     2221 gaagtaccgc ggcaccacgc acggcaccaa cgagtacccg ttcctgatcg acgaggacgg
     2281 gatcaccgtg ctcccgatca ccgcgatgag ccttcagcac gaggtgagcg acgagaggat
     2341 ctcctccggg atcccgcagc tcgacacgat gctgaccggc gagggttact
//
LOCUS       DASUNT010000334         3839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_135575, whole genome shotgun sequence.
ACCESSION   DASUNT010000334 DASUNT010000000
VERSION     DASUNT010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135575"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VFX94_06735"
     CDS             <1..234
                     /locus_tag="VFX94_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767914.1"
                     /translation="DSIMPFVDGKFVVLRLPYPSGLFPKNVDGRIDDDKAGWKGRGIW
                     TTSGTRTNFHNEGGKENRPKAIKLQLRPDPLAR"
     gene            257..883
                     /locus_tag="VFX94_06740"
     CDS             257..883
                     /locus_tag="VFX94_06740"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEX5767915.1"
                     /translation="MTSARWSFEGEYFTACNCDWGCPCNFNARPTEGRCLGWGAWNIT
                     DGRFGSTPLGGKRFALYYCFPGRIEEGAGSACAYVDSRASAEQRRALEAIATGKAGGG
                     IFELFGKALVTRWLPTKFAPIEFEIEDGKGRVRIEGFGEGESELLAYPDGSTIRPQVA
                     LPHGIEYKSGLMTNAKRWWWRDDELLASYANRYGAVARVKFSEEGCVG"
     gene            969..1196
                     /locus_tag="VFX94_06745"
     CDS             969..1196
                     /locus_tag="VFX94_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767916.1"
                     /translation="MSRGELLARVTALMQSCEGCGNVGVVGVTPLDRPDAAGCNWSYT
                     LELETAGVPAEVYGLAYAQVIGIARSSWNLQ"
     gene            complement(1201..1440)
                     /locus_tag="VFX94_06750"
     CDS             complement(1201..1440)
                     /locus_tag="VFX94_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767917.1"
                     /translation="MNTNLSLKARRHAISAIIEARFSVSAFEEVRRKNPQYWSSPRND
                     MARGIYSEAMRAKQRLLDASDELLEAESLAIRLAR"
     gene            complement(1577..2809)
                     /locus_tag="VFX94_06755"
     CDS             complement(1577..2809)
                     /locus_tag="VFX94_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEX5767918.1"
                     /translation="MDAFGAASALITLAALFSWLNQRYIRQPVSVAMLLFSLALSLVL
                     VALGKLGVDSTWLAREAIGAIDLDRALLDGMLSFLLFAGALHVDLAELAKRKWAIGFL
                     ASLGVVISALIAGGALWWLFGVLGHPLPLVWCLLFGALISPTDPIAVLAILKSASAEK
                     SLAAKMAGESLLNDGFGIMLFVLFLGIALGGEEASFASVSLLFAREVLGGALFGLAIG
                     YAAYRLLDALDSYEVEILLTLALVMGGYAAARALHISGPIAVVVAGVLIGNHGLPRAM
                     AARSRERVGQFWALIDEILNAVLFIMIGLELLRLEFNPTYAWAGALAIPAVLAARFLS
                     VGLAALVPGLRQDFPLYVVAILTWGGLRGGISVALALALPSGGFRDAIITVTYMVVLF
                     SILVQGLTLSRLLRRRAA"
     gene            3260..3568
                     /locus_tag="VFX94_06760"
     CDS             3260..3568
                     /locus_tag="VFX94_06760"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5767919.1"
                     /translation="MNLHRPLAVLLLAASLSGGVLAQVQVALDDETIFIAVEHALQGA
                     RSLAGADITVHSRDGYVTLSGFAATMANIAEAGRLASRVRGVTGVRNKIRVAVPPSRV
                     "
     gene            complement(3685..3837)
                     /locus_tag="VFX94_06765"
     CDS             complement(3685..3837)
                     /locus_tag="VFX94_06765"
                     /inference="COORDINATES: protein motif:HMM:NF013555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HEX5767920.1"
                     /translation="MKLATQGDFLTPTCPSCGIKMIPRKSTAHGRTYWGCRNYPGCKH
                     TFYGAS"
BASE COUNT          695 a         1213 c         1395 g          536 t
ORIGIN      
        1 gactcgatca tgccgttcgt cgacggcaag ttcgtggtct tgcgtctgcc ttatccgtcg
       61 gggctcttcc cgaagaacgt ggacgggcgc atcgacgacg acaaagcggg atggaagggc
      121 cgcgggatct ggaccacctc ggggacgcgc accaacttcc acaacgaggg cgggaaggag
      181 aaccgcccga aggcgatcaa gctccaacta aggccggatc cgctggcgcg gtagtcggca
      241 tgagggcgga ggaacgatga cgagcgcgag atggagcttc gagggtgagt atttcaccgc
      301 gtgcaactgc gactggggct gcccctgcaa cttcaatgcg cggccgaccg aggggcgctg
      361 cctgggctgg ggcgcctgga acatcacgga tggccggttc ggctcgacgc cgctcggggg
      421 caagcgcttc gcgctctact actgctttcc gggccggatc gaagagggcg cgggcagcgc
      481 ctgcgcctac gtcgacagcc gcgccagcgc cgagcagcgg cgcgcgctcg aggcgatcgc
      541 caccggcaag gcgggcggcg gcatcttcga gctcttcggc aaggcgctcg tgacgcgctg
      601 gctgcccacc aagttcgcgc cgatcgagtt cgagatcgag gacggcaagg ggcgcgtgcg
      661 catcgagggc ttcggcgaag gcgaaagcga gctcctggcg taccccgacg gcagcaccat
      721 ccggccgcag gtcgcgctgc cgcatggcat cgagtacaag tcggggctga tgaccaacgc
      781 gaagcgctgg tggtggcgcg acgacgagct gctcgcgagc tacgcgaaca ggtacggcgc
      841 ggtggcgcgc gtgaagttca gcgaggaagg ctgcgtcggc tgactttcca gccctgaccg
      901 catccggaaa cacgataagc gacgacatct tttctttcgg cggggacgaa caacgcgaac
      961 gccaggtctt gtcgcgcggc gagctcctcg cgcgcgtgac ggccttgatg caaagctgcg
     1021 agggatgcgg gaacgtgggc gtggtcggcg tcacgccgct cgatcgcccc gatgccgccg
     1081 gctgcaactg gtcgtacacg ctcgagctcg aaacggcggg cgtgccggcg gaagtctatg
     1141 ggctcgccta cgcgcaggtg atcggcatcg cgcggagcag ctggaacctg cagtaagccg
     1201 ctagcgcgcg aggcggatcg ccaggctctc ggcctcgagc agctcgtcgc tcgcgtcgag
     1261 cagccgctgc ttcgcgcgca tcgcttcgct gtagatgccg cgcgccatgt cgttgcgcgg
     1321 cgagctccag tactgcgggt tcttgcgccg cacttcctcg aaggcgctga cgctgaagcg
     1381 cgcctcgatg atggcgctga tggcatgccg gcgcgccttg agcgagagat tcgtgttcat
     1441 gcggcaggat gcgctcccgc gcgcgggggg tctgtgcgct accccaccat gctatggaag
     1501 gggcctggag cgttcgaggc cgcggcgagc atgcatctgg cactacaaca aggtcggcgc
     1561 atagcacatc gggaccctag gcggcacgcc ggcgcaataa tcgcgaaagc gtgagcccct
     1621 gcaccaggat cgagaagagc acgaccatgt aggtgacggt aatgatggcg tcgcggaagc
     1681 cgcccgaggg cagcgcgagg gcaagggcga cggagatgcc gccgcgcagg ccaccccagg
     1741 tgaggatggc gaccacgtag agcgggaaat cctggcgcag gccgggaacg agcgcggcga
     1801 ggccgacgga gaggaagcgc gcggcgagca cggcgggaat ggcgagcgcg ccggcccagg
     1861 cgtaggtcgg gttgaactcg aggcgcaaga gctccaggcc gatcatgatg aagagcaccg
     1921 cgttgaggat ctcgtcgatc agcgcccaga actggcccac ccgctcgcgc gagcgcgccg
     1981 ccatggcgcg cggcaggccg tggttgccga tcaggacgcc ggcgacgacg acggcgatcg
     2041 gaccggagat gtggagcgcg cgcgccgcgg cgtagccgcc catgacgagc gccagggtga
     2101 ggaggatttc gacctcgtag ctgtcgagcg catcgagcag ccggtatgcg gcgtagccga
     2161 tggcgaggcc gaagagcgcg ccgcccagca cctcgcgcgc aaagaggagc gacacgctgg
     2221 cgaagctcgc ctcctcgccg cccagggcga tgccgaggaa gagcacgaag agcatgatgc
     2281 cgaagccgtc gttgaggagc gattcgccgg ccatcttggc ggcgagcgac ttctccgcgc
     2341 tggccgactt gaggatcgcg agcaccgcga tcgggtcggt gggcgagatg agcgcgccga
     2401 agagcaggca ccagacgagc ggcaagggat ggccgaggac gccgaacagc caccacagcg
     2461 ctcccccggc gatgagcgcc gagatcacca cgccgaggct cgcgaggaag ccgatcgccc
     2521 acttgcgctt cgcgagctcg gcgaggtcga cgtgcagcgc gccggcgaag agcaggaacg
     2581 agagcatccc gtcgagcagc gcgcggtcga ggtcgatcgc gccgatcgcc tcgcgcgcga
     2641 gccaggtcga gtcgacgccg agcttgccga gcgcgaccag gacgagcgac agcgcgagcg
     2701 agaagagcag catcgccacg ctcaccggct ggcggatgta gcgctgatta agccagctga
     2761 agagcgcggc gagggtgatc agcgcgctgg cggcgccaaa cgcgtccatg gctcgtcatt
     2821 gtcggggtcg gaacggcctc gagaaaaata aataaaggcc cgcgctgtct ggcgatcggc
     2881 gtatgctggg tcgatgagcc ttcgtcgaga acaacgcccg atcgccagcg gcctcggccg
     2941 cgctttcagc ggagccttcc acgattacgt gcgcgctatg ttcgccgagc attcctcgcg
     3001 ccggcgcctg ctgcgcgcaa ggcgggcgcg ctggctgagc ggtcttgtac agcgccccgc
     3061 gtcgggcgcg gagcagcgct gggaaggcga aggcggaagg acgcggcaga agcgctagac
     3121 gaaatctttc gacgggactg cgaggcgggc gagctgcaaa cgacgagggc gaactctacg
     3181 ctagtccgcc ggcagataac ggcctgcaag aagataaata tagaccggca cggcggcgtc
     3241 atcggagtat gttgggtcga tgaaccttca tcgccccctt gcagttctcc tcctcgcggc
     3301 gagcctctcc ggcggcgtcc tcgcgcaggt ccaagtcgcg ctcgacgacg agacgatatt
     3361 catcgcggtg gagcacgcgc tccaaggcgc gcgttcgctc gccggcgccg acatcacggt
     3421 gcacagccgc gacggctacg tcaccctgag cggcttcgcc gccacgatgg caaacatcgc
     3481 cgaggcgggc aggctcgcct cgcgcgtgcg cggcgtgacc ggcgtcagga acaagatccg
     3541 cgtcgcggtt ccgccttcgc gcgtctgatt cggggtctga cccccaacac ctgctgacgc
     3601 atagctggac agattgcgca tgagttccgc aatccgctcg aggccggcgg cggcatcggg
     3661 aagacgcgct gctaccgaac cgtgctagga agccccgtag aaggtgtgct tgcagccggg
     3721 atagttgcgg cagccccagt acgtcctgcc gtgcgcggtg ctcttgcgcg ggatcatctt
     3781 gatgccgcac gacgggcagg tgggcgtgag gaagtcgccc tgggtcgcga gcttcaaaa
//
LOCUS       DASUNT010000335         3006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_398754, whole genome shotgun sequence.
ACCESSION   DASUNT010000335 DASUNT010000000
VERSION     DASUNT010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398754"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFX94_06770"
     CDS             <1..273
                     /locus_tag="VFX94_06770"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HEX5767921.1"
                     /translation="ALERLPRVLTIHDKAGKVRQTLQLDAYAAETGLKLEPRVIEQHS
                     SLPDGLARDWPPHHAGIEKHEAYALQWYSLAALAVVLVVVLSFRKT"
     gene            270..782
                     /locus_tag="VFX94_06775"
     CDS             270..782
                     /locus_tag="VFX94_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HEX5767922.1"
                     /translation="MNIFHNKLLWVAIVCAAPLVLGTAAYLLRWSPGTPSNYGELVPP
                     TPVAGLEWLRGKWVLVMFDAGACDARCEEKLYFMRQVRRAQGKDMNRVERLWVVTDGA
                     KPRTDIVPGIEGTLIHMQPVAAAFAGPVIEHIYLVDPLGNLMMRFPRDPDPSKMIKDL
                     QRLLRYSRFG"
     gene            907..1716
                     /gene="cyoE"
                     /locus_tag="VFX94_06780"
     CDS             907..1716
                     /gene="cyoE"
                     /locus_tag="VFX94_06780"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX5767923.1"
                     /translation="MLIVFTAVIGMFLATPGMVPLPTLLAATAGIWLVAGAAAAVNCL
                     VEQKIDSVMSRTSWRPLPQGELTAAQTLAFAGALGGVGLWLLYHYVNALTMWLTLATF
                     VGYAIVYTLVLKPATPQNIVIGGASGAMPPLLGWAAVTGEVTIEPLVLFLIIFAWTPP
                     HFWSLALYRTEDYARAGVPMLPVTHGKEYTRLQILLYTLILLAVSLLPYVIRMSGLAY
                     LVAAVALGALFLSYALKIYFAYSDALAQKTFRYSIAYLALLFAALLVDHYL"
     gene            1673..2257
                     /locus_tag="VFX94_06785"
     CDS             1673..2257
                     /locus_tag="VFX94_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX5767924.1"
                     /translation="MRYFSRLFWSIITCSLALAAGCDSRPQFKSTDITGVPYGRSLEL
                     IDHTGKPRRLEDWRGKAVVLFFGFTRCPDVCPTTLADIAQAVKELGADAARVQVLMVS
                     VDPERDTAEALAKYVTAFDAGFVGLRGDLEATRKVAQEFKIYFEKRKQGDTYTVDHSA
                     QSYVIDPEGRLRLLVRHDRIAQDLAPDLRMLLRG"
     gene            complement(2254..2511)
                     /locus_tag="VFX94_06790"
     CDS             complement(2254..2511)
                     /locus_tag="VFX94_06790"
                     /inference="COORDINATES: protein motif:HMM:NF020434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HEX5767925.1"
                     /translation="MRIVLKPLPVAYACQGCPEYGQRARDFAAALDRDGAAEMVWLGG
                     AKEITPTQRYPVFAVDGCSKGCALQWLARRGIAAEQSYVVG"
     gene            complement(2515..>3006)
                     /locus_tag="VFX94_06795"
     CDS             complement(2515..>3006)
                     /locus_tag="VFX94_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HEX5767926.1"
                     /translation="NVNLVLPWVRAADEPEIRQAFEQALAVRQLGAKAKALADRHFFE
                     TLVRVHRAAEGAPYTGLKPAGRDLGPAIPEADRALQQGSVEDLVKLLTEAVRQGVREH
                     FHAAFSRKGFDADDVAAGRAYVEAYVPYVHYVERLWLAASEAAHGLHASRAKAPAHAA
                     HEH"
BASE COUNT          470 a         1044 c          957 g          535 t
ORIGIN      
        1 gcgctcgagc gcttgccacg cgttctgact atccacgaca aagccggcaa ggtgcgccag
       61 acgctgcagc ttgacgccta tgccgcggag accgggctca agctcgagcc gcgcgtgatc
      121 gagcagcact catccctgcc cgacggcctc gcgcgcgact ggccgccgca ccatgccggc
      181 atcgagaagc acgaagccta tgcgctgcag tggtattcgc tcgccgcgct cgccgtcgtg
      241 ctcgtcgtgg tgctgtcctt ccgcaagacg tgaatatctt tcacaacaag ctcctctggg
      301 ttgcaatcgt gtgcgccgcg ccgcttgtgc tcggcacggc ggcgtacttg ctgcgctggt
      361 cgcccggcac gccgtccaac tatggagagc tcgtgccgcc gacgccggtc gccggcctcg
      421 aatggctgcg cggcaagtgg gtgctggtga tgttcgacgc cggcgcctgc gatgcgcgct
      481 gcgaggagaa gctgtacttc atgcgccagg tgcgccgcgc acagggcaag gacatgaatc
      541 gcgtggaacg cctgtgggtc gtcaccgatg gcgcaaaacc gcgcaccgat atcgtgccgg
      601 gcattgaggg aaccctgatc catatgcaac cggtggcggc cgccttcgcc ggtccagtga
      661 tcgaacatat ctacctcgtc gacccgctcg gcaacctgat gatgcgcttc ccgcgcgacc
      721 ccgacccgtc caaaatgatc aaggacctgc aacgcctact caggtattcg cggttcgggt
      781 aaaacgttcg ccgtgctaga cttttgaccc ttcagtgaag cgcctgcctt gaataccacc
      841 gttctttcgc cggggctcac gcagcgcgcg cgcgcgttct acgtgctcac caagccgcgc
      901 gtcgtgatgc tcatcgtctt caccgcggtg atcggcatgt ttctcgccac gccggggatg
      961 gtgccgctgc ccacgctcct cgccgccact gcgggcatct ggctggttgc gggcgccgcg
     1021 gcggcggtga actgcctggt ggagcagaag atcgactcgg taatgagccg cacgagctgg
     1081 cggccgctac cgcagggcga gctcaccgcg gcgcaaacgc tcgctttcgc cggcgcgctc
     1141 ggcggcgtcg ggctgtggct gctctatcac tacgtgaacg cgctcacgat gtggctgacg
     1201 ctcgccactt tcgtcggcta cgcaatcgtc tacaccttgg tcctcaagcc ggcgacgccg
     1261 cagaacatcg tgatcggcgg cgcctcgggc gcgatgccgc cgctgctcgg ctgggccgcc
     1321 gtgactggcg aggtgaccat cgagccgctc gtgcttttcc tcatcatctt cgcctggacg
     1381 ccgccgcact tctggtcgct cgccctctac cggaccgagg actacgcgcg cgccggcgtg
     1441 ccgatgctgc cggtcaccca cggcaaggaa tacacgcggc tgcagatcct gctctacacg
     1501 ctgatcctct tggccgttag cctgctgccc tatgtgatcc gcatgagcgg cctcgcgtat
     1561 ctcgtcgccg ccgtggcgct gggcgcgctg ttcctgtcgt atgccctgaa gatctacttc
     1621 gcttacagcg acgctctggc gcagaagacc ttccgctatt cgatcgccta tcttgcgtta
     1681 cttttcgcgg ctcttctggt cgatcattac ctgtagcctg gcgcttgccg ccggctgcga
     1741 ttcgcgcccg cagttcaaga gcaccgacat caccggggtg ccgtacggac gctccctcga
     1801 gctgatcgac cacaccggaa aaccgcgccg actcgaggac tggcgcggca aggcggtggt
     1861 gcttttcttc ggattcaccc gctgcccgga cgtttgcccc accacgctcg ccgacatcgc
     1921 ccaggcggtg aaggagctcg gcgccgacgc cgctcgcgtc caggtgctga tggtgagcgt
     1981 cgatcccgag cgcgacacag cggaggcgct cgccaagtac gtcaccgcct tcgacgcggg
     2041 attcgtcggc ctgcgcggcg atctcgaggc gacgcgcaag gtcgcgcagg agttcaagat
     2101 ctacttcgag aagcgcaagc agggggacac ctacaccgtc gaccacagcg cgcagagcta
     2161 cgtcatcgac cccgaaggca ggctgcgcct tctcgtgcgg cacgaccgca tcgcgcagga
     2221 cctcgcgccc gacctgcgca tgctgcttcg aggttaaccg accacgtagc tttgttcggc
     2281 agcgatgccg cgccgcgcga gccattgcag ggcgcaaccc ttgctgcacc cgtccacagc
     2341 aaagaccggg tagcgctggg tcggggttat ctccttcgcg ccgccgagcc acaccatctc
     2401 cgccgcgccg tcgcggtcga gcgcggccgc gaagtcgcgt gcgcgctgcc cgtactcggg
     2461 gcagccctgg caggcgtagg cgaccggaag cggcttgagg acgatgcgca tcctctagtg
     2521 ctcgtgggct gcgtgcgcgg gagctttcgc gcgcgacgcg tgaagcccgt gcgccgcctc
     2581 gctcgcagcg agccagaggc gctcgacgta gtgcacataa ggaacatagg cctcgacgta
     2641 ggcgcgacca gccgcgacgt cgtcggcatc gaaccccttg cggctgaagg cggcgtggaa
     2701 gtgctcgcgc acgccttggc gaacggcctc ggtgagcagt ttgacgagat cctccaccga
     2761 gccctgctgc aacgcccggt cggcctcggg aatcgcgggg ccgaggtcgc gtcccgccgg
     2821 cttgagccca gtgtagggcg cgccttcagc ggcgcgatgc acacgcacca gcgtttcgaa
     2881 aaagtgacgg tcggctagcg ctttggcctt cgcgcccagc tgacgaactg ccagcgcttg
     2941 ctcgaaagct tgacggatct cgggctcgtc tgcagcgcgc acccacggca gcaccaaatt
     3001 gacgtt
//
LOCUS       DASUNT010000336         2273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_836690, whole genome shotgun sequence.
ACCESSION   DASUNT010000336 DASUNT010000000
VERSION     DASUNT010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836690"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..349
                     /locus_tag="VFX94_06800"
     CDS             107..349
                     /locus_tag="VFX94_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX5767927.1"
                     /translation="MEQGRLAFTAVYLKSNHGYIGFIEELPGVNSHGRTLDEARETLQ
                     KLAAVVFDEERREAEELIAGKDVVRESFFVPLPRAN"
     gene            463..1986
                     /locus_tag="VFX94_06805"
     CDS             463..1986
                     /locus_tag="VFX94_06805"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEX5767928.1"
                     /translation="MAARNWRTEDWVAVYLGFFIIVLILAAYSGKWFDFGQLRPTFRW
                     TADAQIAAAAPGWRAALDNLAADADTKGRKDLAAAANGVKSALDKGDRAALEKAAGAL
                     AKVGGRNTVPGTLGSEIRGHASSSTGKVFAGQNLLKVLYIGIAGLVIGAVGFALIGGS
                     VGAFMIGFPVVFVLAWLARVIAGNGFFVDYGVEYVIFALLMGLLISNTVGTPKWLMPA
                     VQTEYFIKTGLVILGAGLLFLEVIQAGVLGILQAVLVVVVIWYICFWMARKLRVDDDF
                     AAMLSSSVAICGVSAAIATCGAIQGDKKKLSYVTSLVLIVAEPMMIVMPWIAQAFHFP
                     DMVAGAWLGGTLDTSASVVAAGALISDVAMKTGVIVKFSQNALIGVAAFILVVWWTYK
                     AGAQAGGQQPSAGIIWERFPKFVLGFLVASALFSFFLPTPMVEGTRSVLGELRTWWFA
                     LAFVCIGLETKFVDLATMEGGRPAVAFLGAQAVNIVWTLLLAYLLFGGVIFPLPDIK"
     gene            1994..>2273
                     /locus_tag="VFX94_06810"
     CDS             1994..>2273
                     /locus_tag="VFX94_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767929.1"
                     /translation="MVWELERHGCRSTLIGAAHFFPRHFRRSLRRLIGEARIVLLEGP
                     LDEEATRKVLAAGRGAGGGALYEAAKENIHARLGIVRAPLDLHQLLKDL"
BASE COUNT          358 a          711 c          769 g          435 t
ORIGIN      
        1 ttctgaccac attcgttttg aagtatgagt tccgcgaatt ttttttccgg aagtcatact
       61 tctgcaagct tgggcgcgta accttgtcat tcaacaggag ggacgaatgg aacagggacg
      121 gctcgcgttc accgctgtgt atctgaaatc caaccacggt tacatcggct tcatcgagga
      181 gctccccgga gtgaactcgc atggccgcac cctggacgaa gcgcgcgaga cgctgcagaa
      241 gctcgccgcg gtggtattcg atgaggagcg ccgcgaagcc gaggagctga tcgcgggcaa
      301 agatgtggtg cgcgaatcgt tcttcgtgcc gcttccgcgc gcgaactaag cttattcccg
      361 atcggtgaaa gggcgcctgc gggcgccctt ttcgtttggg gcgtacgata ggcgtgctag
      421 gaggaccaag gcgggacaca aactacaggg agggaccaac gcatggctgc caggaattgg
      481 cgcaccgaag actgggttgc cgtctatctg ggtttcttca tcatcgttct catcctcgcc
      541 gcgtacagcg ggaaatggtt cgacttcggg cagctgcggc caaccttccg ctggaccgcg
      601 gacgcgcaga tcgcggctgc ggcgcccggc tggcgcgcag cgctcgacaa ccttgccgcc
      661 gacgccgaca ccaagggcag gaaagacctc gccgccgccg cgaacggcgt gaagagcgcg
      721 ctcgacaagg gcgaccgcgc ggcgctcgag aaggcggccg gcgcgctggc caaggtcggc
      781 ggtcgcaaca ccgtgccggg cacgcttggc tcggagatcc ggggccacgc atcgtcgagc
      841 accggcaagg tattcgccgg gcagaacctg ctcaaggtgc tctatatcgg catcgccggg
      901 ctggtaatcg gcgcggtcgg ctttgcgctg atcggcggca gcgtcggcgc gttcatgatc
      961 ggcttcccgg tggtgttcgt cctcgcctgg ctcgcgcgcg tgatcgccgg caacgggttt
     1021 ttcgtggact acggcgtgga gtacgtcatc ttcgcgctgc tgatggggct cctgatcagc
     1081 aacaccgtcg gcacgccaaa atggctcatg cccgcggtgc agaccgaata cttcatcaag
     1141 accggcctcg tcatcctcgg cgcaggcctg ctcttcctgg aggtgatcca ggccggcgtg
     1201 ctgggcatcc tgcaggcggt gctcgtggtg gtggtgatct ggtacatctg cttctggatg
     1261 gcgcggaagc tccgcgtcga cgacgacttc gccgcaatgc tctcatcgtc ggtggcgatc
     1321 tgcggcgtgt ccgcggcgat cgcgacctgc ggggcgatcc agggcgacaa gaagaagctc
     1381 tcctacgtca cctcgctggt gctgatcgtc gccgagccga tgatgatcgt catgccgtgg
     1441 atcgcccaag cgttccactt cccggacatg gtggcggggg cgtggctcgg cggcacgctc
     1501 gacaccagcg cctcggtggt cgcggcgggc gcgctcatca gcgacgttgc gatgaagacc
     1561 ggggtaatcg tcaagttctc gcagaacgcg ctgatcggcg tcgctgcctt catcctcgtg
     1621 gtctggtgga cgtacaaggc gggcgcccag gccggcggcc agcagccgag cgccggcatc
     1681 atctgggagc gcttcccgaa gttcgtgctc ggctttctgg tcgcttccgc gctcttctcc
     1741 ttcttcctgc cgacgccgat ggtggagggc acgcgctcgg tgctcgggga gctgcgcacc
     1801 tggtggttcg cgcttgcttt cgtgtgcatc ggcctggaga ccaagttcgt cgacctcgcg
     1861 accatggaag gcgggcgccc agcggtagcg ttcctcggtg cgcaggcggt caacatcgtg
     1921 tggacgctgc tgcttgccta tctgctgttc ggcggcgtca tcttcccgct acccgatatc
     1981 aagtgatctg aagatggtgt gggagctcga gcggcatggc tgccgctcga ctctcatcgg
     2041 ggcggcgcat ttctttccgc gccattttcg ccgcagcctg cgccggctga tcggcgaagc
     2101 gcgcatcgta ctgctcgaag ggccgctcga cgaggaggcc acgcgcaagg tgctcgctgc
     2161 cggccgcggc gccggcggcg gcgcgctgta cgaggcggcg aaggaaaaca tccacgcgag
     2221 gctcggcatc gtgcgcgcgc cgctcgactt gcaccagctc ctcaaggatc tcg
//
LOCUS       DASUNT010000337         3623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_398859, whole genome shotgun sequence.
ACCESSION   DASUNT010000337 DASUNT010000000
VERSION     DASUNT010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398859"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFX94_06815"
     CDS             <1..264
                     /locus_tag="VFX94_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5767930.1"
                     /translation="NLTRALALEWAPDNIRVNAVAPTYVRTDLTVPIFSNPELLKSVM
                     AHTPLGRLPEAEDIAAAIVFLASDAARCITGVTLPVDCGYLAR"
     gene            complement(327..1088)
                     /locus_tag="VFX94_06820"
     CDS             complement(327..1088)
                     /locus_tag="VFX94_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5767931.1"
                     /translation="MNYHAVAAIYRFEMARFRRTLWQSLATPVITTSLYFVVFGAAIG
                     ARMGELEGVPYGAFIVPGLIMLAIFTESLSNASFGIYLPKFTGTIFEVLSAPVSAFEL
                     VLAYVGAAATKSVLLGLVILATANVFVPVSIAHPLWMIAFLVLTATTFCLFGFVLGIW
                     ADGFDQLQFVPMLVITPLTFLGGAFYSIDMLPHAWRAFSLFNPIVYLISGFRWTFYGT
                     ADVPVGASLAVTGAFFLLFLAIVAWIFKTGYRLKT"
     gene            complement(1085..2008)
                     /locus_tag="VFX94_06825"
     CDS             complement(1085..2008)
                     /locus_tag="VFX94_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5767932.1"
                     /translation="MQPVIAISHLTKTYATGLQALKDVSLEIRKGEIFALLGPNGAGK
                     TTLINIVCGIVRASSGSVRVDGHDIVRDYRITRSKIGLVPQELTTDTFETVWATVSFS
                     RGLFGRGSNPPHVEKVLRELSLWERRQDRIRTLSGGMKRRVLIAKALAHEPEILFLDE
                     PTAGVDVELRRDMWALVRGLRASGVTIILTTHYIDEAEEMAERIGVINKGELVVVEEK
                     AALMKKLGKKQLTLHLLEPLTTLPPALAEWKLAIKNHGSELEYTFEASEERTGIPALL
                     RALGELGIAFRDLNTRQSSLEDIFIDLVHQK"
     gene            complement(2018..3415)
                     /locus_tag="VFX94_06830"
     CDS             complement(2018..3415)
                     /locus_tag="VFX94_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5767933.1"
                     /translation="MNTSAQAKATRREWIGLGAIALPCMLYSMDLTVLNLALPALAAE
                     LEPSALELLWIVDIYGFLVAGALITMGALGDRIGRRRLLMIGAGVFGIASIVAAFSTS
                     PAMLIATRAALGIAGATLAPSTFSLIRNMFHDPHERTVAISVWITSYSAGAAIGPLLG
                     GALLQYFWWGSVFLLAVPVMMLLLVLGPALLPEYRDPSAGRIDLASAALSLVAVLALV
                     FGLKHSAQHGFSTLSVLAILIGTGLTAAFVRRQLRLADPLIDLRLFRAPAFSTSLIVY
                     MLGTFVAFGVFVFMTQYLLLVLEMAPLEAGLWTVPFALGFIAGSMVAPRLARALRPAD
                     VMAGGLTVAALGFLLITQAGRVDGPGLVVAGMVLQSIGIAPVFTLTNDLIMSAAPPER
                     AGAASGISETSSELGGALGIALFGSLATAVYGAALDPSTVARALELSAAVSTVLVLGM
                     AAAVVSLLRRAKAAA"
BASE COUNT          671 a         1156 c         1257 g          539 t
ORIGIN      
        1 aaccttacgc gcgcgctcgc gctcgaatgg gcgccggaca acatccgcgt gaacgcggtc
       61 gcgccgacgt acgtgcgcac cgaccttacg gtgccgatct tctccaatcc agagctgctg
      121 aagagcgtca tggcgcacac gccgctcggc cggcttcccg aagccgaaga catcgccgcg
      181 gccatcgtgt ttctggcgag cgatgcggcg cgctgcatca ccggcgtgac cctgccggtg
      241 gattgcggct atctagccag gtagcccgac gtcgaaccgc gcgcgcctct cgcgagcacc
      301 tgctgctcgt cagaccgtgg tcggtgtcag gtcttgagcc ggtagccggt cttgaagatc
      361 caagccacga ttgcgaggaa gaggaggaag aacgcgccgg tgacggcgag gcttgcgccc
      421 accggcacat ccgccgtgcc atagaaagtc cagcggaagc cgctgatgag atacacgatc
      481 gggttgaaca ggctaaacgc gcgccaggcg tgcggcagca tgtcgatcga gtagaacgcg
      541 ccgcccagga aggtgagcgg cgtgatgacg agcatcggca cgaattggag ctggtcgaag
      601 ccgtcggccc agatgccgag gacgaagccg aagaggcaga aagtcgttgc ggtgagtacg
      661 aggaaggcga tcatccagag cggatgcgcg atgctcaccg gcacgaagac gttcgccgtg
      721 gcgaggatca ccaggccgag gagcaccgac ttggtcgctg ccgcgccgac gtaggcgagc
      781 accagctcga acgccgacac cggcgccgac agcacctcga agatcgtgcc ggtgaacttc
      841 ggcaggtaga tgccgaacga ggcgttcgac aggctctcgg tgaagatcgc gagcatgatg
      901 aggccgggaa cgatgaatgc gccgtagggc acgccctcga gctcgcccat gcgcgcgccg
      961 atggccgcgc cgaacaccac gaagtagagc gacgtggtga ttaccggcgt ggcgaggctc
     1021 tgccacaacg tgcgccggaa gcgtgccatc tcgaagcggt agatcgcggc aacggcgtgg
     1081 taattcattt ttggtgcacc agatcgatga agatgtcctc gagcgaggac tgccgggtgt
     1141 tcaagtcgcg gaaggcgatg ccgagctcgc ccagcgcgcg cagcagcgcc gggatgccag
     1201 tgcgctcttc gctcgcctcg aaggtgtatt cgagctcgct gccgtggttc ttgatggcga
     1261 gtttccattc ggcgagcgcg ggcggcagcg tggtcaacgg ttccaggaga tgaagggtga
     1321 gctgtttctt gcccagcttc ttcatgagcg cggccttctc ctcgacgacc accagctcgc
     1381 ccttgttgat gaccccgatg cgctcggcca tctcctcggc ctcgtcgatg tagtgggtgg
     1441 tgaggatgat ggtgacgccg gaggcgcgta gcccgcgcac gagcgcccac atgtcgcgcc
     1501 gcagctcgac gtccacgccc gcggtcggct cgtcgaggaa gagaatctcg ggctcgtgcg
     1561 cgagtgcctt ggcgatcagc acgcgacgct tcatcccgcc ggagagcgtg cggatcctgt
     1621 cctggcgacg ctcccagagc gagagctcgc gcaatacttt ctcgacgtgc ggcggattgc
     1681 tcccgcggcc gaagagtccg cggctgaagc tcaccgtcgc ccacaccgtc tcgaacgtgt
     1741 ccgtggtgag ctcctgcggc acgaggccga tcttcgagcg cgtaatgcgg tagtcgcgca
     1801 cgatgtcatg accgtcgacg cgcacgctgc ccgagctcgc cctgacgatg ccgcagacga
     1861 tgttgataag ggtggtcttg ccggcgccgt tcgggccgag gagcgcgaag atctcgccct
     1921 tgcggatctc gaggcttacg tccttcagcg cctgcagccc ggtggcgtag gtcttggtga
     1981 gatgcgagat cgcgatgacc ggctgcatcc tcgaatacta tgccgccgcc ttcgctcggc
     2041 gcagcaagct gacgacggcc gccgccatcc ccagcaccag aaccgttgag acggccgcgc
     2101 tcagctccag cgcccgcgcc accgtcgaag ggtcgagcgc cgcaccgtag accgcggtcg
     2161 ccaagctgcc gaagagcgcg atgccgagcg cgccgccgag ctcggaggag gtttccgaaa
     2221 tgcccgacgc cgccccggcg cgctcgggcg gcgcggcgga catgatgaga tcgttggtga
     2281 gcgtgaacac cggtgcgatg ccgatcgact gcagcaccat tccggcgacc acgaggcccg
     2341 ggccgtcgac gcggccggcc tgcgtgatga gcagaaagcc gagcgcggcg accgtgagcc
     2401 cgcccgccat gacgtcggcc gggcgcagcg cgcgcgcgag acgcggcgcc accatcgatc
     2461 cggcgatgaa gccgagcgcg aacggtacgg tccacagccc tgcctcgagc ggcgccattt
     2521 cgagcacgag cagcaggtat tgcgtcatga acacgaagac cccgaaagcg acgaacgttc
     2581 cgagcatgta gacgatgagc gaggtgctga aggcgggcgc gcggaagagc cgcaggtcga
     2641 tgagcggatc ggcgagccgc aactggcgac gcacgaacgc ggctgtgagg ccggtgccga
     2701 tcaggattgc gagcacggag agagtcgaaa acccgtgttg cgccgagtgt ttcaagccga
     2761 agacgagcgc gagcaccgcg acgagcgaga gcgccgcgct tgcgagatcg atccggccgg
     2821 cgctcgggtc gcgatactcg ggcaggagcg ccgggccgag gacgaggagc agcatcatca
     2881 ccgggacggc gagcaagaac accgagcccc accagaaata ctggagcagc gcgccgccga
     2941 gcagcgggcc gatcgcggcg ccggccgagt agctcgtgat ccacacgctg atagccaccg
     3001 tccgctcgtg cggatcatga aacatgttgc ggatgagcga gaatgtggac ggggcgagcg
     3061 tcgcgcccgc gatgccgaga gccgctcgcg tcgcgatcag catagccggg ctggtcgaga
     3121 acgcggcgac gatggaggcg ataccaaaca ccccggcgcc gatcatgagc agccgccggc
     3181 ggccgatccg gtcgcccaga gcgcccatcg tgatcagcgc gccggcgacg aggaagccgt
     3241 agatgtcgac gatccacagg agctcgagcg cgctcggctc cagctcggcg gccagcgccg
     3301 gcagcgcgag gttgagcacc gtgaggtcca tcgagtacag catgcagggc agggcgatgg
     3361 cgccgagccc gatccattcg cggcgcgtgg ccttggcttg agcggacgtg ttcatgcgcg
     3421 atggtcgatc ggccaagggc gaaatagacg ccaagtttgc cgctgccgcc gcggctgcgt
     3481 ttcagatgaa acttacgaga tcgagacctc ggccttgtcg gggtagaagg cgagcaagtc
     3541 cttgataccc tcgacttcgt cgccagtcgg cggtttcatc attcgctcct ttccagcagc
     3601 cgcttcgccg cttcgaggtc gcg
//
LOCUS       DASUNT010000338         5877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_486474, whole genome shotgun sequence.
ACCESSION   DASUNT010000338 DASUNT010000000
VERSION     DASUNT010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486474"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VFX94_06835"
     CDS             complement(<1..728)
                     /locus_tag="VFX94_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5767934.1"
                     /translation="MDIRGFRLTEAPEDFLDDPYPYYAALRESSPVHELESGSYFVSR
                     YEDVVAVYRHAGASSDKKVEFKPKFGDSPLYEHHTTSLVFNDPPLHTRVRALIMGALN
                     QRAIARMETGVATLVEKLLDRFSTEVEFIGEFAARVPVEVIGNLLDIPRDERGPLRAW
                     SIAILSGLEPRPTPEMLEHGNRAVSEFKDYLARLVEERRKHPGDYESDVLTRLIQGEK
                     DGARLSTLELYHNCIFLLNAGHETT"
     gene            747..1280
                     /locus_tag="VFX94_06840"
     CDS             747..1280
                     /locus_tag="VFX94_06840"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5767935.1"
                     /translation="MPRVPVADFEPQLKKRLEELWGTPPNLYKALGNHPELVAAWTEF
                     SRTLRYDTRTPRALRELVILRGAQLMGSEYEWAQHLSMARKAGVREAQIGSLSAWRTS
                     SQFDAREKAALALGEAVTLGRVSDEVHAMAMKEFDAHDYVEIALVAAFYAMVGRMLDA
                     MGVELEPEMRSYAPKLA"
     gene            1307..1972
                     /locus_tag="VFX94_06845"
     CDS             1307..1972
                     /locus_tag="VFX94_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEX5767936.1"
                     /translation="MAAFVTVLICSNLIGPAKAVQLDLPLVGAVVFGAGVLFFPISYV
                     FGDILTEVYGYARARRVIWAGFAGLAFASFMAYVVVALPPAPKWPNQQAYEIAFGSTW
                     RIAAASMFAYFCGEFANSFVLAKMKILTAGKWLWTRTIGSTVVGEAVDSSLFYPLAFY
                     NSGIMPNEIIPGLMVAQFVAKVVVEVAFTPVTYKIVGALKRTEHEDYYDRNTDFTPFS
                     LRT"
     gene            2045..2716
                     /locus_tag="VFX94_06850"
     CDS             2045..2716
                     /locus_tag="VFX94_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767937.1"
                     /translation="MAKRTHRSADFRLGELLTRAGLLPRSRLDAALERQATHNGRLGA
                     LLVELGLIDEAELYGVLALQTSLHQAESADVILFLRARLGDILLGAAAITEHQLLRAL
                     LQQECSGEPLGEILLRQGVITLAVREGALGFQRTLSSPLRERFRLGRMLLEANILDST
                     TLEAAIRRQRGARVKLGDALLEMGAITQDVLEGVLKRQRRLLAALAAGMAVAADAYGL
                     PARGS"
     gene            complement(2790..>5877)
                     /locus_tag="VFX94_06855"
     CDS             complement(2790..>5877)
                     /locus_tag="VFX94_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX5767938.1"
                     /translation="GDDHGAKSSTLPHQSEHIFKACLIPVLNPSNVQDYLDLGLHGFA
                     MSRYSGCWIAFKCVTDIVESGASVEVNPDRIQVSIPQDFRLPPDGLNVRWPDGILEQE
                     ARILDWKVYAALAYVRANGLDRIIFDSPKARLGIVTTGKSYGDVMQALADLGIDKEAA
                     RQIGLRVYKVAMSWPLEPTGARRFAEGLEEILVVEEKRQLIEYQIKEELYNWKEGVRA
                     PRVVGKFDDSGEWSRSGGQPAGTWLLPAHYEHSPAMVAKAIAQRLAKLGMAEKLGSQF
                     RERLAFLEFKEKLLAKPRVAAIRQPYFCSGCPHNTSTRVPEGSRATAGIGCHFMAVWM
                     NRSTATFTHMGAEGAPWIGQAPFTNEKHIFANLGDGTYFHSGILAIRAAVAAKVNMTY
                     KILFNDAVAMTGGQRHDGPLDPPIISRQLAAEGVTPIVVVTDEPDKYPRGTPWAAGVT
                     VRHRDELDSVQRELRDTPGVSALIYDQTCASEKRRRRKRNEYPDPAKRVVINEMVCEG
                     CGDCSVKSNCLSVEPLETEFGRKRQINQSSCNKDYSCLKGFCPSFVTVEGGRLKKGES
                     AFRPLEGDFPSMPDPQASALSSPYGILVTGIGGTGVITIGQIIGVAAHLEGKGVTVLD
                     MSGLAQKYGAVMSHVQIAAGPGQTHATRIPTGGADLLLGCDLVVSASTEAVEKMAHGR
                     TRALVNASVTPTAEFVRNPNWQLPGSDLQKDIADSCKEADFVAAAEIATGLMGDAIAT
                     NMFMLGYAYQKGWIPLSAASLERAIELNGVAVDFNRKSFVWGRRAALDLERVRRLAIP
                     AEVISIEQRFSRNLDELVERRVRFLAEYQNPAYAARYQALVQRTAQVERERVNSTKLT
                     EAVARYYAKLLAYKDEYEVARLHAGVGFRAKIEGMFEGDYRVAYHLSPPLFARKDPNS
                     GEPRKTRFGPWVLPAFRVLSSLKFLRGTPLDIFGYTEERRTERALIREYEETLARLLA
                     GLDAQNHALAVQIASIPEEIRGFGHIKARSIPPARKKRDELLARYEPGRGDQRAAA"
BASE COUNT          929 a         1936 c         1944 g         1068 t
ORIGIN      
        1 gtcgtctcgt ggccggcgtt caggaggaat atgcagttgt ggtagagctc gagcgttgaa
       61 agccgcgcgc cgtccttctc gccctggatg agccgcgtga ggacgtcgct ctcgtagtcg
      121 cccgggtgct tgcgccgctc ctccaccagg cgcgcgagat agtccttgaa ctcgctgacc
      181 gcgcggttgc cgtgctcgag catctcgggc gtgggccgcg gctcgaggcc ggagagaatc
      241 gcgatcgacc aggcgcgcaa cggcccccgc tcatcgcgcg ggatgtcgag caggttgccg
      301 atcacttcca ccggaacgcg agctgcgaac tcgccgatga attccacttc ggttgaaaaa
      361 cgatcgagca gtttttcaac cagcgtcgcc accccggttt ccatccgggc gatggcgcgc
      421 tggttgagcg ctcccatgat gagcgcgcgc acccgcgtgt ggagcggcgg gtcgttgaag
      481 acgaggctcg tggtgtgatg ctcgtagagc ggcgagtcgc cgaacttggg cttgaactcg
      541 accttcttgt cggagctcgc gccggcgtgg cggtagaccg ccaccacgtc ctcgtagcgc
      601 gagacgaaat agctgccgct ctccagctcg tgcaccggag agctctcgcg cagcgcggcg
      661 tagtagggat acgggtcgtc caggaaatcc tccggcgcct cggtgagccg gaagccgcgg
      721 atatccatca gttattatcc gccctcatgc cacgcgtgcc tgtcgcggac ttcgagccgc
      781 agctcaagaa gcgcctggaa gagctgtggg gcacgccgcc caacctgtac aaggcgctcg
      841 gcaaccatcc cgagctcgtt gcggcatgga ccgaattttc ccggacgctg cgctacgaca
      901 cgcgcacgcc gcgcgcgctg cgcgagctcg tgatcctgcg cggcgcgcag ctcatgggct
      961 cggagtacga gtgggcgcag cacctctcca tggcgcgcaa ggccggcgtg cgcgaagcac
     1021 agatcggcag cttgtccgcc tggagaacgt cttcgcagtt cgatgcgcgc gagaaagcgg
     1081 cgctcgctct cggcgaggcg gtgacgctgg gccgggtgag cgatgaagtc catgccatgg
     1141 ccatgaaaga gttcgacgcc cacgactacg tcgagatcgc gctcgtcgct gccttctatg
     1201 ccatggtcgg acgcatgctc gacgccatgg gcgtggagct cgagcccgag atgcgcagct
     1261 atgctccaaa gcttgcttga aaacctaccg ctattacgag ttcgtgatgg cggcgttcgt
     1321 cacggtgctc atctgctcca acctgatcgg gccggcgaag gccgtccagc tcgatctccc
     1381 gctcgtgggc gcggtggtgt tcggagcagg cgtgctcttc ttccccattt cctacgtgtt
     1441 cggcgacatc ctgaccgagg tctacggcta tgcgcgcgcg cggcgcgtca tatgggccgg
     1501 attcgccggg ctcgcgttcg cgtcgttcat ggcttacgtg gtggtcgcgc tgccgcccgc
     1561 gccgaagtgg ccgaaccagc aagcctacga gatcgccttc ggcagtacct ggcgcatcgc
     1621 ggcggcatcg atgttcgcct acttctgcgg cgagttcgcc aactccttcg tcctcgccaa
     1681 gatgaagatc ctcaccgccg gcaagtggct gtggacgcgc accatcggct caaccgtggt
     1741 cggggaggcg gtcgactcgt cgctcttcta cccgcttgcc ttctacaact ccggaatcat
     1801 gccgaacgag atcattcccg gtctcatggt ggcgcagttc gtcgccaaag tggtggtgga
     1861 agtcgctttc acccccgtca cctacaagat cgtgggggcg ctcaagcgca ccgagcacga
     1921 ggactattac gaccgaaaca ccgatttcac tcccttcagc ctcaggacat aatctcacta
     1981 gttcactagc cgagtgcgtc acagttcctc ggcccgggga ggaggggggg acatcatcgc
     2041 ggcgttggcc aaacggacac accgcagcgc tgattttcgc ctcggcgagc ttctcactcg
     2101 ggcaggcctg ctcccgcgct cgcgcctcga tgcggcgctc gagcgccagg cgacgcacaa
     2161 cggcaggctc ggcgccctgc tcgtggagct cggcctgatc gacgaggcgg agctctacgg
     2221 cgtgctcgcc ctgcagacta gtttgcacca ggccgagtcc gcagacgtga tccttttcct
     2281 gcgcgcgcgc ctgggcgaca tcctgctcgg cgccgccgcc atcaccgaac accagctcct
     2341 gcgcgcgctg ctgcagcagg agtgcagcgg cgagccgctc ggcgagatcc tgctgcgcca
     2401 gggggtgatc actctcgccg tgcgggaagg agccctcggc ttccagcgca ccctgagcag
     2461 cccgctgcgc gagcgcttcc gcctcggccg catgctgctc gaagcgaaca tcctggattc
     2521 gacgacgctc gaggcggcga tccgccgcca gcgcggcgcc cgggtcaagc tcggcgacgc
     2581 gctcctcgag atgggcgcca ttacgcagga cgtgctcgaa ggcgtactca agcgccagcg
     2641 ccgcctcctc gccgcgctcg ccgcgggtat ggcagtcgcg gccgacgcat acggattgcc
     2701 agcccggggc agctaacaca aagacgggcg acagcaaaat ccggggtctg accccaacaa
     2761 gaaagccggg gtcagacccc gactcttttc tatgccgccg cgcgctggtc gccccgcccg
     2821 ggctcgtagc gcgcgaggag ctcgtcgcgc ttcttgcggg cggggggtat ggagcgcgcc
     2881 ttgatgtggc cgaagccgcg gatctcctcg gggatcgagg cgatctgaac ggcgagcgcg
     2941 tggttctgcg cgtcgaggcc ggcgagcagc cgcgcgagcg tttcctcgta ctcccggatg
     3001 agcgcccgct cggtgcgccg ctcctcggtg tagccgaata tgtcgagcgg ggttcctcgc
     3061 aggaacttca atgaagagag gactctgaag gccggcaaga cccatggccc gaagcgggtc
     3121 ttgcgaggct cgccgctgtt gggatccttg cgggcgaaaa gcggcggcga gaggtggtac
     3181 gcgacgcggt agtcgccttc gaacatgcct tcgatcttcg cgcggaagcc cacgccggca
     3241 tgcagccgcg ccacctcgta ttcgtccttg taggcgagca gcttggcgta gtagcgcgct
     3301 accgcctcgg taagtttcgt ggagttgacc cgctctcttt ccacttgggc cgttctttga
     3361 accaacgcct ggtatcgcgc ggcgtaagcg gggttttggt attcggcgag gaacctgacg
     3421 cgccgctcga cgagctcatc gaggttacgc gagaaacgct gctcgatcga gataacttcg
     3481 gcggggatcg cgaggcgcct gacgcgctcg aggtccagcg ccgcgcgccg gccccagacg
     3541 aagctcttcc tgttgaagtc caccgcgacg ccgttcagct cgatcgcgcg ctcgagcgag
     3601 gctgccgaga gcgggatcca gcccttctgg taggcgtagc ccagcatgaa catgttggtc
     3661 gcgatcgcgt cgcccatcag gccggtggca atctccgcgg ccgcgacgaa gtccgcttcc
     3721 ttgcacgaat cggcgatgtc cttctgcagg tcgctgcccg gcagctgcca gttcggatta
     3781 cgcacgaatt ccgcggtcgg ggtgaccgag gcattgacaa gcgcgcgcgt gcgcccgtgc
     3841 gccatctttt ccaccgcctc ggtgctcgcg cttaccacga gatcgcagcc gaggagcaga
     3901 tcggcgccgc cggtggggat gcgcgtggcg tgcgtctgcc cggggccggc ggcgatctgc
     3961 acgtgcgaca tgaccgcgcc gtacttctgc gcgagccccg acatgtcgag cacggtgacg
     4021 cccttgcctt cgaggtgcgc cgcgacgccg atgatctggc cgatcgtgat gacccccgtg
     4081 cctcctatcc cggtcacgag aattccgtac ggcgagctaa gcgctgaggc ctgcgggtcc
     4141 ggcattgaag ggaaatcccc ttccaggggc ctgaacgcgg actcgccttt cttcaggcgc
     4201 ccgccttcca ccgtcacgaa gcttgggcag aagcccttca ggcaggagta gtccttgttg
     4261 cacgaggact ggttgatctg gcgcttgcgg ccgaactcgg tctcgagcgg ctcgaccgag
     4321 aggcagttcg acttcacgct gcagtcgccg cagccctcgc acaccatctc gttgatgacg
     4381 acgcgcttcg ccgggtccgg gtactcgttt ctcttgcggc ggcggcgctt ctcggaagcg
     4441 caggtctggt cgtagatgag cgccgagacg cccggcgtgt cgcgcagctc gcgctgcacg
     4501 ctgtcgagct cgtcgcggtg gcggaccgtc acgccggcgg cccacggcgt gccgcgcggg
     4561 tacttgtccg gctcgtcggt gaccaccacg atcggcgtca cgccctcggc ggcgagctgg
     4621 cgcgagatta tcggcgggtc gagcggaccg tcgtggcgct ggccgcccgt catcgcgacc
     4681 gcgtcgttga agaggatctt gtaggtcatg ttgaccttcg ccgccactgc cgcgcgtatc
     4741 gcgaggatcc ccgagtggaa gtacgtgccg tcgcccaggt tcgcgaagat gtgcttttcg
     4801 ttcgtgaacg gcgcctgccc gatccacggc gcgccctcgg cgcccatgtg ggtaaaggtg
     4861 gcggtcgagc ggttcatcca caccgccatg aagtggcaac cgatgcccgc ggtggcgcgg
     4921 cttccctcgg gaacgcgcgt ggaggtgttg tgcgggcagc cggagcagaa gtaaggctgc
     4981 cggatggccg ccaccctcgg cttggccaga agtttctcct tgaattcgag gaatgcgagc
     5041 cgctcgcgga actgcgagcc gagcttctcg gccattccaa gctttgcgag tctttgcgcg
     5101 atcgccttgg ccaccatcgc gggcgaatgc tcgtaatgcg cgggcaggag ccaggtaccg
     5161 gcgggctgac cgcccgagcg gctccactcg cccgagtcgt caaacttgcc gacgacgcgc
     5221 ggcgcgcgca cgccttcctt ccagttgtag agctcttcct tgatctggta ctcgatgagc
     5281 tggcgcttct cttccaccac caggatttct tccagcccct cggcgaagcg gcgagcgccc
     5341 gtgggctcga gcggccagga catcgccacc ttgtagacgc gcaggccgat ctgccttgca
     5401 gcttctttat cgatacccag atcggccagt gcctgcatca catcgccgta cgatttgcct
     5461 gtggtgacga tgccgagcct tgccttcggg gaatcgaaaa tgatcctgtc gaggccgttg
     5521 gcccgcacat aggcgagcgc ggcgtagacc ttccagtcga ggatgcgcgc ttcctgctcg
     5581 aggatgccgt cgggccagcg cacgttcagg ccgtcgggcg gaaggcggaa atcctgcgga
     5641 atgctcacct gaatgcggtc tggattcact tcgaccgaag ccccggattc gacgatgtcg
     5701 gtcacgcact tgaacgcgat ccagcacccc gaatagcggc tcatcgcgaa cccgtggagg
     5761 cccagatcga ggtagtcctg cacgttggaa gggttgagca ccgggatgag gcaggccttg
     5821 aagatgtgct ccgactggtg cgggagcgtg gaggacttcg cgccgtggtc gtcgcct
//
LOCUS       DASUNT010000339         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1187688, whole genome shotgun sequence.
ACCESSION   DASUNT010000339 DASUNT010000000
VERSION     DASUNT010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187688"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..79
                     /locus_tag="VFX94_06860"
     CDS             <1..79
                     /locus_tag="VFX94_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HEX5767939.1"
                     /translation="QWAVTPGQSVVLYDGEVCLGGGVIT"
     gene            complement(72..689)
                     /locus_tag="VFX94_06865"
     CDS             complement(72..689)
                     /locus_tag="VFX94_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5767940.1"
                     /translation="MKLLASTASPYTRKVRIVLTEKKIDCEMEQVDVAAMDNPVNAYN
                     PLGKIPTLVLDDGTALYDSRVIVEFLDGKSPISRLIPDDLRERVAVRRWEALADGVLD
                     AGLLVRYESLREAHERSQAWTDKQLARIKRGMTQMASEHAERPWCHSERYSLADIAVG
                     CCLGWLDFRKPGAVDWLREYPGLAKHYEKLMGRPAFADTVPQAPK"
     gene            complement(701..1279)
                     /locus_tag="VFX94_06870"
     CDS             complement(701..1279)
                     /locus_tag="VFX94_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX5767941.1"
                     /translation="MRISLSAVSREARACTACADELPFAPRPVFRVGRSARLLIVGQA
                     PGRRVHETGIPWNDPSGEVLREWLALERPAFYDTSRIAIVPVGLCYPGTVDGADLPPC
                     RGCAPRWQPLFRAALPGIRLTLLVGGYAQAFYLQRRRKKTLADTVRSWLDYLPEFFPL
                     PHPSWRNIGWLERNPWFAKDVLPALRRRVRTV"
     gene            1299..>2065
                     /locus_tag="VFX94_06875"
     CDS             1299..>2065
                     /locus_tag="VFX94_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX5767942.1"
                     /translation="MPSPLAALSPLDGRYSSGADPLRGYFSEQALIRYRTRLELDWLQ
                     ALAAERSIGELKPFSRRTAASFASLIRNFSEGDAAEIKAIEAQTNHDVKAIELWLKRK
                     LAKSAEAQLALEFIHFACTSEDVNNLAYALMIADSRANVMLPRLDELVAALRAAARRH
                     AALPMLSRTHGQAATPTTLGKEMANFAHRLARARERIAAVRPLGKINGAVGNYNAHVA
                     AYPGFDWERFCRRFVERLGLEFNPYTTQIEPHDWLAEL"
BASE COUNT          335 a          703 c          705 g          322 t
ORIGIN      
        1 gcagtgggcg gtgacgccgg ggcagtcggt cgttctctac gacggcgagg tgtgcctcgg
       61 cggcggcgtg attacttagg agcctgcggg acggtgtcgg cgaaggccgg ccggcccatc
      121 agcttttcgt agtgcttcgc aagccccggg tactcacgca gccagtccac ggctccgggc
      181 ttgcggaagt cgagccagcc gaggcagcag ccgaccgcga tgtcggcgag cgaatagcgc
      241 tcgctgtggc accacggccg ctcggcatgc tcggaggcca tctgcgtcat gccgcgcttg
      301 atgcgcgcga gctgcttgtc ggtccaggcc tggctgcgct cgtgcgcctc gcgcagcgac
      361 tcgtagcgca caagcagccc ggcgtcgagc acgccgtcgg cgagcgcctc ccagcggcgc
      421 accgcgacgc gctcgcgcag atcgtcgggg atgagccggc tgatgggcga cttgccgtcc
      481 aggaattcga cgatcacgcg agagtcgtag agcgcggtgc cgtcgtcgag cacgagcgtc
      541 gggatcttgc cgagcgggtt gtacgcgttg accgggttgt ccatcgcggc gacgtccacc
      601 tgctccatct cgcagtcgat cttcttctcg gtgaggacga tgcgcacctt tcgtgtatac
      661 gggctggcgg tcgaggcgag aagcttcatg aagcgggcga ttatacggtc cggacccggc
      721 ggcgcagggc cgggagtacg tcttttgcga accagggatt gcgctcgagc catccgatgt
      781 tgcgccagct cggatgcggc agaggaaaga actccggcag gtaatcaagc catgaccgta
      841 cagtgtctgc cagcgtcttc tttctgcgcc gctgcagata gaaagcctgg gcataaccac
      901 cgacgaggag cgtgaggcgg atgccgggaa gcgcggcgcg gaagagcggc tgccagcgcg
      961 gcgcgcatcc gcggcacggc ggcaggtccg cgccgtccac ggtgccggga taacagaggc
     1021 ccaccgggac gatggcgatg cgcgaggtgt cgtagaacgc cggccgctcg agcgcgagcc
     1081 actcgcgcag cacttcgccg gaaggatcgt tccacgggat cccggtctcg tgcacgcgcc
     1141 gccccggcgc ctggccgacg atgagcaggc gcgcgctcct gccgacgcgg aaaaccggcc
     1201 tcggcgcgaa cggcaattca tccgcgcacg cggtgcaggc gcgcgcttcg cggctcaccg
     1261 cggagagcga gattcgcatc cccttataat agaacgccat gccttcgccg ctcgccgcgc
     1321 tgtctcctct cgacgggcgg tactccagcg gcgccgatcc tctgcgcggc tacttcagcg
     1381 agcaggcgct catccgctac cgcacgcgcc tcgagctcga ctggctgcag gcgcttgctg
     1441 ccgaacgctc gatcggggaa ttgaagccgt tttcgaggag aaccgctgca agctttgcgt
     1501 cgctgatccg caacttttcc gagggcgatg ccgccgagat caaggcgatc gaggcgcaga
     1561 ccaaccacga cgtcaaggcg atcgagctgt ggctgaagcg aaagcttgcg aagagcgccg
     1621 aagcgcagct cgcgctcgag ttcatccact tcgcctgcac ctccgaggac gtcaataacc
     1681 tcgcctacgc gctgatgatc gccgactcgc gcgccaacgt gatgctgccg cggctcgacg
     1741 agctcgtcgc cgccctgcgc gccgcggccc gccggcacgc ggcgctgccg atgctttcgc
     1801 gcacgcacgg ccaggcggcg accccgacga cgctcggcaa ggagatggcc aacttcgcgc
     1861 accggctcgc gcgcgcgcga gaacgcatcg ccgccgtccg gccgctcggc aagatcaacg
     1921 gcgccgtggg caactacaac gctcacgtcg cggcgtatcc cgggttcgac tgggagcgct
     1981 tctgccgccg cttcgtcgag cgcctcgggc tcgagttcaa cccctatacc acgcagatcg
     2041 agccgcacga ctggcttgcc gagct
//
LOCUS       DASUNT010000340         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_924308, whole genome shotgun sequence.
ACCESSION   DASUNT010000340 DASUNT010000000
VERSION     DASUNT010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924308"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VFX94_06880"
     CDS             <1..628
                     /locus_tag="VFX94_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HEX5767943.1"
                     /translation="FKPQRRIVEAELPSPEVKLELMTLAIGRLTRAGYLYIGMDHFAR
                     PDDELAAAQKLGKLSRSFEGYSTHSGDLIGVGLSSIGQVGPIYYQNVKELERYYAALD
                     AGRLPVLRGIELTSDDLARRAVIQALMCHFRVSIESIELSYVVDFRRHFAPELHMLKK
                     LADEGLVEVQPDWIVVTPKGRLLVRAIAMLFDRYLRDARERASYSKVI"
     gene            625..1323
                     /locus_tag="VFX94_06885"
                     /pseudo
     CDS             625..1323
                     /locus_tag="VFX94_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(1320..1538)
                     /locus_tag="VFX94_06890"
     CDS             complement(1320..1538)
                     /locus_tag="VFX94_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767944.1"
                     /translation="MHYRYFTLEQRDHLEQAIRDRANDPTDLALALERLQMPDYGTCA
                     ACGVEMLYVELIDAPAATVCPACRVEAS"
     gene            1631..2125
                     /locus_tag="VFX94_06895"
     CDS             1631..2125
                     /locus_tag="VFX94_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEX5767945.1"
                     /translation="MNPLTLYAHAVAIEREAVARYSEFAERMSDLGNDAAAEVFATLA
                     KMEGEHLEELNRRTAGLALPALADGKYSWLDAAAPETAARELVFRLMTPRHALEIALA
                     GEKRAAAFFEHVILTADDPMLRALATELAADEGDHIVMLEKLLERTPSPIVDWASIYE
                     RDTA"
BASE COUNT          349 a          776 c          753 g          376 t
ORIGIN      
        1 cttcaagccg cagcggcgca tcgtcgaggc ggagctgcct tcgcccgaag tcaagctcga
       61 gctcatgacg cttgccatcg ggcgcctgac gcgcgcgggc tacctgtaca tcggcatgga
      121 ccacttcgcg cgccccgacg acgagctcgc cgcggcgcaa aagctcggca agctctcgcg
      181 cagcttcgag ggctattcga cccactcggg cgacctgatc ggcgtggggc tttcctcgat
      241 cgggcaggtg ggcccgatct actaccagaa cgtcaaggag ctcgagcgct actacgccgc
      301 gctggacgcg ggacggctgc cggtgctgcg cggcatcgag ttgacctcgg acgacctcgc
      361 gcggcgggcg gtgatccagg cgctgatgtg ccacttccgc gtctcgatcg agtcgatcga
      421 gctctcatac gtcgtcgatt tccggcgtca cttcgccccc gagctgcaca tgctgaaaaa
      481 gctggccgac gaaggcctgg tcgaggtgca gcccgactgg atcgtggtaa cgccgaaggg
      541 ccggctcctc gtgcgcgcga tcgcgatgct cttcgaccgt tacctgcgcg atgcgcgcga
      601 gcgcgcgagc tactccaagg tcatatgacg gaatttgcac tgccgttggc gctcttcgcc
      661 ctcgggcttg cgtcgggcct gcactgcgcc ggcatgtgcg gcgggatcag cgccgccttc
      721 acgctggtgc acaaggaccg gatctggaag cgtcagctcg cgttcaacgc aggccgcatc
      781 acgagctacg ccgtcgccgg cgccgtcgcc ggcgcgctcg gcagcgcggg cgcctacgcc
      841 gcggccgccc tgcccgttca atccgttctc tacttccttg cgagcgcgct cgccgtgctc
      901 gccggcatcc agctcagcgg aatcgcgtta ccgctttcgc ggctggaaag gttcggcacg
      961 cccgtctggc ggcgcataca gccgctggcg gcccgcctgc ttcccgcacg aaccctgccg
     1021 cgggcgtacg ccgcagggct cgcgtagggt tggcttcctt gcggcctcgt ctatggcgcg
     1081 cttgctgcgg cggtgttctc gggcggggtg gcgcacggcg cgcttgccat ggcggcattc
     1141 ggcgccggca cgctgccctg gctgttcctg accggtgtcg cggctgcgaa gctgcgcgga
     1201 tggacgggcg tcacgacgct acgccggctg agcggagcgc tcctcatcgc gctcggcggc
     1261 ttgggcgtgg cacacgcgag cggcctgggc gagagcgtct ggcagacgct cgcctgcttt
     1321 taactcgcct cgacccggca agccggacaa accgtcgccg caggcgcgtc gatcagctcg
     1381 acgtagagca tttcgacacc gcaggcggcg caggtgccat agtccggcat ctgcagccgc
     1441 tcgagcgcaa gcgcaagatc ggtcggatcg ttggcccgat cgcggatcgc ctgctcgaga
     1501 tggtcgcgct gctcgagggt gaaataacgg tagtgcataa gccctccttg ggaaaaatcc
     1561 tacggtagcg cccggcggcg ggtttgacga agatcaatac cggcccggcc ggatgcctca
     1621 tcgttgcggc atgaacccgc tcacgctata cgcccacgcc gtcgccatcg aacgcgaagc
     1681 cgttgcgcgc tactccgagt tcgccgagcg catgtcggat ctcggcaacg acgccgcggc
     1741 ggaagttttc gccaccctcg cgaaaatgga gggcgagcac ctcgaggagc tgaaccggcg
     1801 aaccgcagga ctcgcgctgc ccgcgcttgc cgatggcaag tacagctggc tcgatgccgc
     1861 cgccccggag accgccgcgc gcgagctcgt gttccgcctg atgacgccgc gccacgcgct
     1921 cgagatcgca cttgccggcg agaagcgcgc cgccgcattt ttcgagcacg tcatcctgac
     1981 cgccgacgac cccatgctgc gcgcgctggc taccgagctc gcggccgacg agggcgacca
     2041 catcgtgatg ttggagaagc tcctggagcg cacgccgagc ccgatcgtcg actgggcgtc
     2101 gatctatgaa agggataccg catgaaggac gccatttcca tcctgaagag cgagcatcgg
     2161 tcgatctccg cggtactgca cgggctgaaa gagcttgcac gcatggcgca ggactccaag
     2221 gtgaggccgc gcttccaggt gctgcgcagc atgc
//
LOCUS       DASUNT010000341         2734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_661693, whole genome shotgun sequence.
ACCESSION   DASUNT010000341 DASUNT010000000
VERSION     DASUNT010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661693"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /gene="hemE"
                     /locus_tag="VFX94_06900"
     CDS             <1..889
                     /gene="hemE"
                     /locus_tag="VFX94_06900"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX5767946.1"
                     /translation="ALAAEVTLQPLERFPLDAAIVFSDILTIPDAMGLGLAFAEGEGP
                     RFERPLRNEADIRKLAAPDPVSELGYVLDAVRESRAAIAGRVPLIGFSGSPFTLACYM
                     VEGAGSDDWRALKTLLYTRPDLLHRILQVNTTAVAEYLNAQIEAGADAVMLFDTWGGI
                     LAHEDYETFSLAYVRQVFAKVRKAPTILFTKGGSPWLAAMMHSGCSAVGLDWTCDARE
                     ARRLAAGRVALQGNLDPAALFAPPERVREAARKVIDSFGREPGHIFNLGHGLQPRTPV
                     ESVAVLVDEVRAYSARRRA"
     gene            1088..>2734
                     /gene="priA"
                     /locus_tag="VFX94_06905"
     CDS             1088..>2734
                     /gene="priA"
                     /locus_tag="VFX94_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX5767947.1"
                     /translation="MPDREFFCVRIALDVPLQKLFEYSLPDGVDARVGDRVAVRFGAQ
                     QKIGVVVEERVTPELPASRIKPINALRDDAPRLPEDWIGFMRFLSGYYQRPLGETLIG
                     ALPPRLRSIKPLPRKVLKQEPQPAGARFVAKHELTEAQARVVQRIAGGFSAFLLHGVT
                     GSGKTEVYLHLVARTLERGMQALVLVPEISLTPQLEARFREAFPDARLALMHSGLEDV
                     ARTGAWLAAARGEAGIVLGTRLAVLAPLPKLALIVVDEEHDSSFKQQEGLRYSARDAA
                     VYRAKLAACPVVLGSATPSLETWHNCTLGRYAKLELPERASPGARLPAVATVDMRDDP
                     AEQGFCKKLISAIARRLEKRDQSLVFINRRGYAPVLACEACGWTAGCERCTARMVLHA
                     ADRRLRCHHCGAEAAIPRACPTCGNVDLKAMGRGTQRVEETLAARFPGARIVRIDRDS
                     ARRRAELSRTLEGIRRGEGDILVGTQLLAKGHDFPALTLVGVLNADTALVSTDYRAPE
                     RLFSVLAQVAGRAGRRERPGEVLIQTRYPGHPLFAALARHD"
BASE COUNT          444 a          930 c          900 g          460 t
ORIGIN      
        1 cgcgctcgcc gccgaagtga cgctccagcc tctggagcgc ttcccgctcg atgccgcgat
       61 cgtgttctcg gacatcctca ccatacccga tgcgatggga ctcggactcg cgtttgcgga
      121 aggcgaaggt ccgcgcttcg agcggccgct gcggaatgaa gcggacatcc gcaagctggc
      181 ggcgccggat ccggtgagcg agctcgggta tgtgctcgac gcagtgcgcg agagccgggc
      241 ggccatcgcc ggccgcgttc cgctgatcgg cttttcaggc agccctttca cgcttgcctg
      301 ctacatggtg gagggcgccg gaagcgatga ctggcgcgcc ctcaagacgt tgctttacac
      361 gcgccccgat ctcctgcacc gaatcctcca ggtcaatacg acagccgtcg ccgagtacct
      421 caacgcgcaa atcgaggccg gcgcggatgc ggtcatgctg ttcgacacat ggggcggcat
      481 cctcgcccat gaggactacg agaccttttc gcttgcgtat gtgcggcaag ttttcgccaa
      541 agtgcgaaag gcaccgacga ttctcttcac caaaggcgga agcccgtggc tagcagcgat
      601 gatgcatagt ggttgcagcg ccgtgggcct tgactggacc tgcgatgcgc gcgaagcgcg
      661 ccggcttgcg gcgggacgcg tcgcgcttca aggcaacctg gaccccgcgg cgctcttcgc
      721 gccgcccgag cgcgtgcgcg aagccgcgcg caaggtaatc gacagcttcg gtcgcgagcc
      781 cggccatatc ttcaacctag gccatggatt gcagccgcgt acccccgttg aatcggtagc
      841 ggtgctcgta gacgaagtgc gcgcttacag cgctcgccgg cgcgcttgac ttatgcacat
      901 cttcggctcc ttgccgggaa tttaccgata gtacggccgg tccctgcgta gggagctata
      961 agcgcctgaa tccgcaatgt aaaaccatta cgtcggccgg cgtcgcccgg gacacgttcc
     1021 agtgcccgga gcatgtgaca cgaggatgaa ggccttaccc acaggtttat ccacaaacac
     1081 taagtccttg ccggacaggg agtttttttg cgtccggatc gcgctcgacg tgccgctgca
     1141 gaagctcttc gagtattcgc tccccgacgg ggtcgatgcg cgcgtgggcg atcgcgtagc
     1201 cgtgcgcttc ggcgcacagc aaaagatcgg cgtggtggtg gaagagcgcg taacacccga
     1261 gcttcctgca tcgcgcatca agccgatcaa cgcgctgcgc gacgatgcgc cgcgccttcc
     1321 cgaagactgg atcgggttca tgcgctttct gtcgggctac taccagcggc cgctcgggga
     1381 gaccctgatc ggcgcgttgc cgccgcgcct gcgctctatc aagccgctgc cgcgcaaggt
     1441 gctgaagcaa gagccgcagc cggctggcgc acgctttgtc gcgaagcacg agctcaccga
     1501 ggcgcaagcg cgcgtcgttc agcgcatcgc cggcggtttc agcgctttcc tgcttcacgg
     1561 agtgacgggt agcggcaaga ccgaggttta cctgcacctc gttgccagga cgctcgaacg
     1621 cggaatgcag gccctggtgc tcgttccgga gatcagcctc acgccgcagc tcgaagcgcg
     1681 ctttcgcgaa gcgtttcccg acgcgcgcct cgcgctcatg cacagcggcc tggaggacgt
     1741 ggcgcgcacc ggcgcctggc ttgccgcggc gcgcggcgag gcgggaatcg ttctcggcac
     1801 ccggctcgcg gtgctcgcgc cgctgccgaa gctcgcgctc atcgtcgtgg acgaggagca
     1861 cgattcgtcc ttcaagcagc aggaaggact gcgctactcg gcgcgcgacg cagcggtata
     1921 ccgcgcgaaa ctcgctgcct gcccggtggt cctgggaagc gccacgccgt cgctcgagac
     1981 gtggcacaac tgcacgctcg ggcgctacgc gaagctcgaa ttgcccgagc gcgcctcgcc
     2041 cggcgcacgg ctcccggccg tggcgacggt cgacatgcgc gacgatcctg ccgagcaagg
     2101 cttctgcaag aagttgattt ccgcaattgc acgacgactc gagaagcgag accaaagcct
     2161 ggtgttcatc aatcgccggg gctatgcccc cgtgctcgcg tgcgaggcgt gcggctggac
     2221 cgcgggctgt gagcgctgca ccgcgcgcat ggtgctccac gcggccgatc gaaggctgcg
     2281 ttgccatcac tgcggcgccg aagccgccat cccgcgcgca tgcccgacct gcggcaacgt
     2341 cgatctgaaa gccatgggac gaggaacgca gcgcgtcgag gagacgctcg cggcgcgctt
     2401 ccccggggcg cgtatcgtgc gcatcgaccg cgacagcgcg cgccggcgcg ccgagctttc
     2461 gcgcacgctc gaaggcatcc ggcgcggcga aggcgacatc cttgtcggga cccagctcct
     2521 cgccaagggg cacgatttcc cggcgctcac gctcgtcggc gtgctgaacg cggataccgc
     2581 gctcgtctcg accgattacc gcgcgcccga gcgcctgttt tcggtgctgg ctcaggttgc
     2641 cggacgcgcc gggcgccgcg agcgccctgg cgaggtgctg attcaaaccc gctacccggg
     2701 ccacccgctt ttcgccgcgc tcgcgcgcca cgac
//
LOCUS       DASUNT010000342         5075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1100368, whole genome shotgun sequence.
ACCESSION   DASUNT010000342 DASUNT010000000
VERSION     DASUNT010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100368"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFX94_06910"
     CDS             <1..324
                     /locus_tag="VFX94_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5767948.1"
                     /translation="IDAPNPAIRAFAEKYEKEFRSKSDHNGIKGYTGMYVVKAVTEKI
                     GKFDRKAFAQAIKNSCFSAKQTPGLLMDVCFDDKGDIDRESFLVEVKGGKQVVVETLP
                     PLKRQ"
     gene            324..1085
                     /locus_tag="VFX94_06915"
     CDS             324..1085
                     /locus_tag="VFX94_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX5767949.1"
                     /translation="MRAISLAALTVLELPAHEQVSVAVQAGYSHVGLRLVPVANQVLP
                     PFDEREVERRLAATGVRVLDVEVFRLAPQTRVEDWKAAFAAAQRLGASDLLAHGDDPV
                     PQRLAHALGELCELAARYGLRVNLEPMPWVEISTVTRAKRLIAESGVDNAAVLIDAIH
                     FYRADNRYEDIPPALNYAQICDAHPGQPVDMQEIMRQARSDRLFPGEGALDLKRLLGA
                     LPAELPLSVEVPVSRPMSPLDRARRAQNALQRLLS"
     gene            1082..1963
                     /locus_tag="VFX94_06920"
     CDS             1082..1963
                     /locus_tag="VFX94_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEX5767950.1"
                     /translation="MIIENQEDVTRKVLEEMHRTPDARTRDILEALVTHLHAFVREVR
                     LTEAEFQAAIGYINAIGQKTTPSHNEAMLLAGALGVSNLVCLLNNRHPTQANNLGPFF
                     RDGAPRCENGASLLRSPTPGAPLQFKGWVRDAAGDPVAGAEVDVWHSSTVGLYENQDP
                     TQADMNLRGKFVTAADGSFGFRSVKPAGYPVPTDGPTGALLAAQRRHNMRPAHLHFLI
                     YKPGFKTIASQVYDPEDPHLETDSQFGVTGALVGEYVKHGDGSYALEFTFVLEPGEAR
                     RPRAPITGKAGPAGKTA"
     gene            complement(1926..2660)
                     /locus_tag="VFX94_06925"
     CDS             complement(1926..2660)
                     /locus_tag="VFX94_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HEX5767951.1"
                     /translation="MGWLTNWRRQRVLRKHRIEEALWREATGRLRFIPQTPKLRSLAL
                     LFLAEKQFSGTHGLEVTDEMRVSIAAQACLPVLELGLDWYAGFSGIVVYPGDFRVRRS
                     ETDEDGVVHEWDDELAGEAMPGGPVVLSWDATANDAGMNVVIHEFAHKLDMLNGAADG
                     LPPLHAAMDRAAWAGAFEEAYQGFCDAVERGKETWLDPYAAEHASEFFAVISEAFFED
                     PAETRRRYPDVYDQLKLFYRQDPLSR"
     gene            2678..4795
                     /locus_tag="VFX94_06930"
     CDS             2678..4795
                     /locus_tag="VFX94_06930"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX5767952.1"
                     /translation="MVSVVQLHPQSGASALEPLAAGLAAPERDLVARALDFAEPLYAG
                     QRLSTGEPAWSHALGIAASLAAIGLDAPGRGAGILFASPKYLDGLDKLREAFGEEIAA
                     LAGGVEKLYQLRVATRGTPREQNEVLRKMVLGMVEDVRVVLIRLASRTQTLRYFAKSS
                     SPEKVSYARETLDIYAPLANRLGVWQLKWELEDLSFRFLEPELYKRIAQMLDEKRLER
                     ERYIANAMETLSRELGALHITGQITGRPKHIYSIWNKMRTKQLDFSELYDVRALRVIV
                     PEVKDCYAALGVVHNLWQPIPREFDDYISRPKGNLYQSLHTAVVGPGGKTLEVQIRTE
                     EMHRQAEYGIAAHWQYKEKAKPSQQFDQKVAWLRQLLAWRDEVPVKKPELEDSIYVLT
                     PQGKVIDLPAGSTPIDFAYALHSDLGHRCRGARVDGHIVKLDTPLASGQRVEIVAAKT
                     GGPSRDWLNPERGFVKSARARHKIRQWFNAKALADTVAAGRAVVEKELRRERATQASL
                     EGLATKLGFGKPDELFAAVARDEVNLRQLQIALKGAVPAAPELKKARKAPSAKAAMLV
                     VGMGGLATQLARCCKPVPPDIIRGFVTRGKGVSIHRESCESLKRLAEQYPERVMETAW
                     GRSEAAYAVDMVVTAADRRGLLRDIGDALAREGINVTAVRTQSRDEVAFMRFSFEVVD
                     AAQLKRALGLVRSVKGVIRVARA"
BASE COUNT          906 a         1684 c         1678 g          807 t
ORIGIN      
        1 atcgacgcgc ccaaccccgc gatccgtgcg ttcgcggaaa aatacgaaaa ggaatttagg
       61 agcaagtcgg accacaacgg catcaaggga tacaccggaa tgtacgtagt gaaggcggtt
      121 accgagaaga tcggcaagtt cgaccgcaag gcgttcgcgc aggcgatcaa gaactcgtgc
      181 ttcagcgcca agcagacgcc ggggctgctg atggacgtgt gcttcgacga caagggcgac
      241 atcgaccgcg agagcttcct cgtcgaggtg aagggcggga agcaggtggt agtggagacg
      301 ctgccgccgc tgaagaggca gtagtgcgcg caatcagtct cgcggccctc acggtcctcg
      361 agctcccggc gcacgagcag gtctcggtcg cggtgcaggc cggctattcg cacgtcggcc
      421 tgcgtctcgt gccggtggcg aaccaggtcc tgccgccctt cgatgagcgc gaggtcgagc
      481 gccggctcgc ggccaccggg gtgcgtgtgc tcgatgtcga ggtgtttcgc ctggcgccgc
      541 aaacgcgggt cgaagactgg aaagcggcgt tcgccgccgc gcagcgactg ggcgctagcg
      601 acttgctggc gcacggcgac gatccggtgc cccagcgact tgctcacgcc ttgggcgagc
      661 tgtgcgagct tgcggcgcgt tacggcctga gggtgaacct ggagccgatg ccatgggtcg
      721 aaatttccac cgtgacgcgg gcaaagcgcc ttatcgccga gtcgggcgtg gacaatgccg
      781 ccgtcctgat cgatgcgatc catttttatc gcgccgacaa tcgctacgaa gacatcccgc
      841 cggcattgaa ctatgcacag atctgcgacg cgcacccggg gcagcctgtc gacatgcagg
      901 aaatcatgcg ccaagcgcgc tcggaccggc tcttccctgg cgagggcgcg ctcgacctga
      961 aacgtctcct cggggccctg ccggctgagc tcccgctgag cgtcgaagta ccggtctcgc
     1021 gcccgatgag cccgttggat cgcgcgcggc gggcgcagaa cgctttgcaa aggctcttgt
     1081 catgatcatc gaaaaccagg aagacgtcac gcgcaaggtg ctggaggaaa tgcatcgcac
     1141 gcccgatgcg cgcaccaggg acatactcga ggcgctcgtg acccacctgc acgctttcgt
     1201 gcgcgaagtt cgccttaccg aggccgagtt ccaggcggcg atcggctaca tcaacgcgat
     1261 cggacagaag accaccccga gccacaacga ggcgatgctg ctcgcgggag cgctaggcgt
     1321 ttcgaacctc gtgtgcctgc tcaacaaccg ccacccgacc caggccaaca atctcgggcc
     1381 gttcttccgc gacggtgcgc cgcgctgcga gaacggcgcc tcgctgctgc gctcgccgac
     1441 gccaggcgcg ccgctccagt tcaagggctg ggtgcgcgat gccgcgggtg acccggtggc
     1501 gggggcggaa gtcgatgtct ggcattcctc taccgtcggg ctctacgaga accaggaccc
     1561 gacccaggcg gacatgaacc tgcgcggcaa gttcgtgacc gccgccgacg gctcgttcgg
     1621 gttccgcagc gtgaagccgg ccggctaccc ggtgccgacc gacggcccga caggcgcgct
     1681 gctcgcggcg cagcgcaggc acaacatgcg ccccgcgcat ctgcactttc tcatctataa
     1741 gcctggcttc aagacgatcg cctcgcaggt ctacgacccg gaagatccgc acctcgagac
     1801 cgacagccag ttcggcgtca ccggcgcgct ggtcggtgaa tacgtcaagc acggcgatgg
     1861 ctcctacgcg ctcgagttca cgttcgtgct ggagcccggt gaggcccgcc ggccgagagc
     1921 gccgatcacc gggaaagcgg gtcctgccgg taaaacagct tgagctgatc gtagacgtcg
     1981 ggatagcgcc gccgcgtctc ggccgggtct tcgaagaacg cttcgctgat cacggcgaaa
     2041 aactccgacg catgctcggc ggcgtaggga tcgagccagg tctccttgcc gcgttctacc
     2101 gcatcgcaga agccttggta cgcctcttcg aatgcgccgg cccaggccgc gcggtccatc
     2161 gcggcgtgca gcggcggcag gccgtcggct gcgccgttca gcatgtccag cttgtgcgcg
     2221 aattcgtgga tcaccacatt catcccggca tcgttggcgg tcgcgtccca cgacaggacc
     2281 accggcccgc cgggcatcgc ttcgcccgcg agctcgtcgt cccactcgtg caccacgccg
     2341 tcctcgtcgg tctcgctgcg tcgcacgcgg aaatcgccgg ggtagacgac gatgccgctg
     2401 aagccggcgt accaatcgag gccgagctcg aggaccggca ggcacgcctg cgcggcgatc
     2461 gagacgcgca tttcatcagt cacctccagg ccgtgcgtgc cgctgaactg cttctcggcg
     2521 aggaaaagca gcgcgaggct gcgcagcttc ggcgtctgtg gaatgaagcg caatcgaccg
     2581 gtcgcttcgc gccacagcgc ttcctcgata cgatgcttgc gcagcacgcg ctgccgccgc
     2641 cagttcgtca gccagcccac ggcccgtaaa atacctcatg gtttccgtcg tccagctgca
     2701 cccgcagtcg ggtgcgagtg cgctcgagcc gctcgccgcc gggctcgcag cgccggaacg
     2761 cgacctcgtc gcccgcgcgc tcgacttcgc cgagccgctt tacgccgggc agcggctatc
     2821 gaccggcgag ccggcgtgga gccacgcgct tgggattgcg gcgagcctgg cggcgatcgg
     2881 gctcgacgcg ccaggccgcg gcgccgggat cctgttcgcc tccccgaaat acctcgacgg
     2941 cctcgacaag ctgcgcgagg cgttcggcga ggagattgcc gcgctcgccg gcggcgtgga
     3001 aaagctctac cagcttcggg tcgcgacccg aggcacgcca cgcgagcaga acgaagtgct
     3061 gcgcaagatg gtcctcggca tggtcgagga tgtgcgcgtc gtgctgatcc ggctggcgag
     3121 ccgtacccag acgctgcggt atttcgcgaa aagctcctcc cccgagaagg tttcgtacgc
     3181 gcgagagacg ctcgacatct atgccccgct cgccaaccgg ctcggcgtgt ggcagctcaa
     3241 gtgggagctc gaggacctct cgttccggtt tctcgaaccc gagctctata aacgcatcgc
     3301 gcagatgctc gatgaaaagc gcctcgagcg cgagcgctac atcgccaatg ccatggagac
     3361 gctcagccgg gagctcggcg cgctgcacat caccgggcag attaccggaa ggcccaagca
     3421 catctattcc atctggaaca agatgcgcac caagcagctg gatttttccg agctctacga
     3481 cgtgcgcgcg ctccgcgtca tcgtgcccga agtcaaggac tgctacgccg cgctcggcgt
     3541 agtgcacaat ctctggcagc cgattcccag ggaattcgac gactacatct cgcgcccgaa
     3601 gggaaacctt taccagtcgc tgcacaccgc ggtggtcggc ccgggcggca agaccctaga
     3661 ggttcagatc cgtaccgagg agatgcatcg ccaggccgag tacggcatcg ccgcgcactg
     3721 gcaatacaag gagaaggcaa agccgtcgca gcagttcgac cagaaggtgg cctggctgcg
     3781 gcaactcctc gcatggcgcg acgaggtgcc ggtcaagaaa cccgagctcg aggacagcat
     3841 ttacgtgctt acgccgcagg gcaaggtaat cgacctgccg gcgggctcga caccgatcga
     3901 cttcgcatac gcgctgcaca gcgaccttgg ccatcgctgc cgcggcgcgc gcgtcgacgg
     3961 gcatatcgtc aagctcgaca cgccgctcgc gagcggccag cgggtggaga tcgttgccgc
     4021 gaagaccggc ggcccctcgc gcgactggct caaccccgag cgcggttttg tgaagagcgc
     4081 acgcgcgcgg cacaagatcc gccagtggtt caacgccaag gcgctcgccg acaccgtggc
     4141 cgcgggccgc gcagtggtcg aaaaggagct gcgccgcgaa cgcgccacgc aggcgagcct
     4201 cgaggggctc gcaaccaagc tcggtttcgg caagcccgac gagcttttcg ctgcggtggc
     4261 gcgcgacgag gtcaacctga ggcagctgca gatcgccttg aagggcgctg tccccgcggc
     4321 gccggaactg aagaaagcgc ggaaagcacc gagcgcaaaa gcggccatgc tggtggtcgg
     4381 catgggcggg ctcgcgaccc aactggcgcg ctgctgcaaa ccggtgccgc ccgacatcat
     4441 ccgcggcttc gtcacgcgcg gcaagggcgt atccatccat cgcgagagct gcgagagcct
     4501 gaagcgcctg gcggagcagt atcccgagcg cgtgatggag actgcctggg gcagaagcga
     4561 ggccgcttac gcggtcgaca tggtggtcac ggccgccgat cgccgcggac tgcttcgcga
     4621 catcggcgac gccctcgcgc gcgaaggtat caacgtgacg gcggtgcgca cgcagtctcg
     4681 cgacgaggtc gctttcatgc gcttcagctt cgaggttgtc gatgcggctc agctcaagcg
     4741 cgcgcttggg cttgtgcgca gcgtcaaggg cgtgatccgc gtggcgcgcg cttgatccag
     4801 gagacgtaga tccaggccgg gctcgcgccc ttccggcgct gcgtgtgacg gtgcagcgct
     4861 cgccacggtc tgcgtcgcaa gccgcggttg gccatcgtgg ctaaaataag cttgcaagga
     4921 gagaggaatc catgaatcca gctgcgcaat cgcagatgct gctcattgga cgcgtgctgc
     4981 tcggcgtgct gtttgttgtc gccgggattc ggatcatcct gaactattcc atgaacatcg
     5041 ggtatttcac caaactcggc ttcccggcgc ccgag
//
LOCUS       DASUNT010000343         3064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_48298, whole genome shotgun sequence.
ACCESSION   DASUNT010000343 DASUNT010000000
VERSION     DASUNT010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48298"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VFX94_06935"
     CDS             <1..682
                     /locus_tag="VFX94_06935"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5767953.1"
                     /translation="VNPYVQKSLPYDTEKDFAPVTNLTSSGGLFLAVHPSLPVNTLKE
                     FLEYARRPEAKLAYSTPGIGNNWHLATEVFSKVAGIKMTHVPYKGGGPAAAALVSGEV
                     QAMLVSPAPIMPHYKGNKVRVLAYSGEKRNAAAPEVPLMKEAGVDYTYDGGWFGMFAP
                     ARTPPEILERIAAEVRTAMKKPAVLERFASLGVEPAAGTPEEFRKFFLAELKAYGEMA
                     RVAGVKPE"
     gene            complement(669..1496)
                     /locus_tag="VFX94_06940"
     CDS             complement(669..1496)
                     /locus_tag="VFX94_06940"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate 1,2-dioxygenase"
                     /protein_id="HEX5767954.1"
                     /translation="MASLVGAYAASHGPLLVREWEALPQDQKQRLERAFAQLGQRIAA
                     ARPDALIVVSPDHWSNFFLDNVPAVCIGVGESNEGPPEPWMKGFPHREIPGHPALALH
                     IVREAMSEGFEPSVSHRLKLDHGICIPLWRMGLERLPRIVPMLVNSIEPPLPSLRRCL
                     EWGRQLARAVKSYPEPIKVSILATGGLSHSIGEPTMGAIYEDFDRETLRLFSSGERDL
                     ISYLEKNLPSHGNGSEEVRNWLVAHGAAGTRGFALVDYLPVPKVIVGCGFASWTIPA"
     gene            complement(1489..1734)
                     /locus_tag="VFX94_06945"
     CDS             complement(1489..1734)
                     /locus_tag="VFX94_06945"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767955.1"
                     /translation="MTDYWLSKMFFDLQSPAAAAEYRADREKVLARYGLTPEVKQAVL
                     ANDVSFLSRRTNPYLLRYFFFAAGMKDDEFIRRLRDG"
     gene            1768..2205
                     /locus_tag="VFX94_06950"
     CDS             1768..2205
                     /locus_tag="VFX94_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5767956.1"
                     /translation="MTSIEVNGTRHDVAAALDTPLLYVLRNHLGLMGTRFGCGSGQCG
                     ACFVLIDGRAMASCDMPLSFAVDRKITTNEGLNGEHRVQKALERHQAAQCGYCMSGIV
                     MSAIALLASETSPSEDAVRAALDKNLCRCGSHNRVVRAILDAR"
     gene            2195..>3064
                     /locus_tag="VFX94_06955"
     CDS             2195..>3064
                     /locus_tag="VFX94_06955"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5767957.1"
                     /translation="MLAEKPPALSDARRLSQRVHFERDGTVSVASGKVELGQGILTAL
                     AQIAAEELGVPLEKIRMLPASTAHSPDEGVTSGSLSIQDAGKALRKACAELRALLKMS
                     GAASYWDLELATDIPGDAPEKPAAEYRIVGTSAPRLDLPGKFAGRPSYVQDMVLPGML
                     HARVVRPAFAFKALLSVEEPKNAKLVRDGNFLGVLAEREEDAIAAAAKLRAKCAWEPA
                     PPMPDDIHAWLKQNVAEHIVSKEKSDPAAAARGAKRVAASYTKPYIAHASIGPSCALA
                     RWSEGKVEVWTHSQ"
BASE COUNT          566 a          992 c         1028 g          478 t
ORIGIN      
        1 ggtgaacccc tacgtgcaga aatcgctgcc ctacgatacc gagaaagact tcgccccggt
       61 gaccaatctc acgagcagcg gcggcctgtt cctcgcggtg cacccgtcgc tcccggtcaa
      121 cacgctgaag gagttcctcg agtacgcgcg gcggcccgaa gccaagctcg cctacagcac
      181 gcccggcatc ggcaacaact ggcatctcgc caccgaagtg ttcagcaagg tcgccggcat
      241 caagatgacc catgttccgt acaagggcgg cgggccggcg gccgcggcgc tcgtgtccgg
      301 cgaagtgcag gcgatgctgg tgtcgccggc gccgatcatg ccgcactaca agggcaacaa
      361 ggtgcgcgtg ctggcctaca gcggcgagaa gcgcaacgca gccgcgcccg aggttccgct
      421 catgaaggag gcgggcgtcg actacaccta cgacggcggc tggttcggca tgtttgcgcc
      481 ggcgcgcacg ccgcccgaga tcctggagag aatcgccgcc gaggtgcgca cggcgatgaa
      541 gaagccggcg gtgctcgagc gcttcgcgag cctgggcgtg gaacccgcgg ccggcacgcc
      601 agaggaattc cgcaagttct tcctcgccga gctcaaggcc tacggcgaga tggcgcgcgt
      661 cgcgggcgtc aagccggaat agtccaggac gcgaagccgc agccgacgat caccttcggc
      721 accggcaggt agtcgacgag cgcgaagccg cgcgttccgg ctgcgccgtg cgccaccagc
      781 cagttgcgca cctcctccga gccgttgccg tgcgacggca ggttcttttc gagatacgag
      841 atcaagtcgc gttccccgga agagaagagc cgaagcgttt cgcggtcgaa gtcctcgtag
      901 atcgccccca tggtcggctc gccgatggag tggctcaggc cgccggtcgc aagaatcgaa
      961 accttgatcg gttccggata agacttcacg gcgcgggcaa gctgccgacc ccactccagg
     1021 caacgccgca acgaaggcaa gggcggctcg atgctgttta cgagcatcgg cacgatgcgc
     1081 ggcaggcgct cgaggcccat gcgccacagc gggatgcaaa tgccgtggtc gagcttgagc
     1141 cggtgcgaca ccgaaggctc gaagccctcg ctcatcgctt cgcgcacgat gtggagcgcg
     1201 agcgccgggt gaccgggaat ctcgcgatgc gggaagcctt tcatccacgg ctctggcggg
     1261 ccttcgttcg actctcccac gccaatgcag accgccggga cgttatcgag aaagaaattc
     1321 gaccaatggt cgggcgagac gacgatgagc gcgtcgggcc gcgcggcggc gatgcgctgc
     1381 ccgagctggg cgaaagcgcg ctcgagcctc tgcttctggt cctgcggcag cgcttcccac
     1441 tcgcgcacga gaagcggacc gtggctggcc gcgtaggcgc cgaccaggct agccatcgcg
     1501 cagcctgcgg atgaactcgt cgtccttcat cccggcggcg aaaaaaaagt agcgcaggag
     1561 gtaggggttc gtgcgcctgg agagaaagga gacatcgttc gcgagaaccg cctgcttcac
     1621 ttccggtgta aggccgtatc tcgccagaac cttttcccgg tcggcgcgat actccgccgc
     1681 ggctgcgggg ctctgcaaat cgaagaacat tttcgaaagc cagtaatcgg tcatcgccgg
     1741 tcgagtttat gccaaaatct tttcgcgatg acatccattg aagtgaacgg cacgcgccac
     1801 gacgtcgccg cggcgctgga cacgccgctc ctctacgtgc tgcgcaacca tctcgggctc
     1861 atgggcacgc gcttcggctg cggcagcggc cagtgcggcg cgtgcttcgt gctcatcgac
     1921 ggccgcgcca tggcgtcttg cgacatgccg ctctcttttg ctgtcgacag aaagataacc
     1981 actaacgaag ggctgaacgg cgaacatcgg gtgcagaagg cgctggagcg ccaccaggcg
     2041 gcgcaatgcg gctattgcat gagcggcatc gtgatgagcg ccatcgccct tctcgcgagc
     2101 gaaacatcgc cgtcggaaga cgcggtccgg gctgcgctcg acaagaacct ctgccgctgc
     2161 ggatcgcaca accgcgtggt gagggcgatc ctcgatgctc gctgagaagc cgcccgcgct
     2221 gtcggatgcg aggcgcctgt cgcagcgcgt tcattttgag cgcgacggca cggtatcggt
     2281 ggcgagcggc aaggtcgagc tcgggcaggg catcctgact gcgcttgcgc agatcgcggc
     2341 ggaagagctt ggcgtaccgc tggagaagat ccgcatgctt cccgcgagca ccgctcacag
     2401 tccggacgag ggcgtgacct cgggcagcct ctcgatccag gacgccggca aagcgctgcg
     2461 caaggcgtgc gccgagcttc gcgcgctgct gaagatgagc ggcgcggcct cgtactggga
     2521 tctcgagctc gcgaccgata ttccgggaga cgcgccggaa aagccggcgg ctgaataccg
     2581 catcgtcggc acgagcgcgc cgcggctcga tctgccaggc aaattcgcgg gccggccgag
     2641 ctatgtccag gacatggtgc tccctggcat gttgcacgca cgcgtcgtac gtcccgcatt
     2701 cgctttcaag gcacttctct cggtcgaaga accgaaaaac gcgaagctgg tacgagacgg
     2761 caatttcctc ggcgtgcttg ccgagcgcga agaggatgcg atcgccgcgg cggcgaagct
     2821 gcgcgcgaaa tgcgcgtggg agccggcgcc gcccatgccg gacgatatcc atgcctggct
     2881 caagcagaac gtcgccgagc acatcgtctc caaggagaag tccgatccgg cggctgccgc
     2941 gcgcggcgcg aagcgtgtcg cggcgtcgta tacgaagccg tacatcgccc atgcttccat
     3001 cggaccgtcg tgcgcgctcg cgcgctggag cgagggcaag gtcgaggtat ggacgcacag
     3061 tcag
//
LOCUS       DASUNT010000344         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_48335, whole genome shotgun sequence.
ACCESSION   DASUNT010000344 DASUNT010000000
VERSION     DASUNT010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48335"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..835
                     /locus_tag="VFX94_06960"
     CDS             59..835
                     /locus_tag="VFX94_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5767958.1"
                     /translation="MRRRFERAAATYAKSSRLEGEVATRMLERLDYVKLAPRRVLDAG
                     SGPPRQELARRYPKAQVVALDFALPMLRAVRRGWFRKHSILLCSDIGRMPLAAGSFEL
                     VWCNMALHWLEEPLPAFREFSRVLAPEGLLVFSTLGPDTLKELRAAAGAERVHQFIDM
                     HDLGDMLVGAGFASPVMDMEMIQVDYGRGDGLVADLRSSGQTSARADRRRGLGGRAMR
                     ARLRAALPPRVTFEVVYGHAWKGRKVPPEVKSVRVFKRMP"
     gene            872..1312
                     /locus_tag="VFX94_06965"
     CDS             872..1312
                     /locus_tag="VFX94_06965"
                     /inference="COORDINATES: protein motif:HMM:NF021517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HEX5767959.1"
                     /translation="MDTPQFSRTLKRNCAMSPAGLARVFAALALLVLAIGAGFAVAGA
                     WLVLPFAGLEVLLLGAAFVAHARHAADYEKIELHSGRLAVEVAQAERVARYELENARV
                     SIEEGRVVLRDARKELEIGRHIGAEARAELAAELEKTLFQGRTT"
     gene            1309..>2256
                     /gene="coxB"
                     /locus_tag="VFX94_06970"
     CDS             1309..>2256
                     /gene="coxB"
                     /locus_tag="VFX94_06970"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX5767960.1"
                     /translation="MKGSKPVPRALAVLAGLAAAAAPGAAHAIAWYFQTPASKMAQDI
                     DTLHQVIMWIIVVIFIGVFTFMFYACYAHRKSIGHKAAQFHENTTVELLWTIIPALIL
                     IIIAWPVTKVVIAQKDTSAPDLTVKVTGYQWKWGYDYLRGEGEGISFVSNLATPRDQI
                     EGKAPKGENYLLEVDNEMVVPVGKKIRVITTAADVIHAWWIPAFGAKQDAIPGFLRDT
                     WFRAEQPGTYRGQCTELCGKEHAFMPIVVRAVSEADYTKWVGERKKLAGPAQAAAPVA
                     SAAAASAAPAPAASAAAPAKPAADDSKPLPLAELVSRGEK"
BASE COUNT          361 a          735 c          782 g          378 t
ORIGIN      
        1 atcctctcgg tcgcggcggc gattgaaaga gcaatcaatt cggcttgatc cacgcgccgt
       61 gcggcgcagg ttcgagcgcg cggcggccac gtacgcgaag tcttcccggc tggagggaga
      121 agtcgcgacg cgcatgctcg agcggctcga ctatgtcaag ctcgcgcctc gccgtgttct
      181 cgatgcgggc agcggtccgc cgcggcagga gctcgcgagg cgctacccga aggcgcaggt
      241 cgtcgcgctc gattttgcgc tgcccatgct gcgagcggtc cgccgcggct ggtttcgcaa
      301 gcattccatt cttttatgca gcgatatcgg ccgcatgccg ctcgccgccg ggtcgttcga
      361 gctcgtatgg tgcaacatgg cgctgcactg gctcgaggag ccgttgccgg cgttcaggga
      421 gttctcccgc gtgctcgcgc cggagggcct gctggtcttc agcacgctcg ggccggacac
      481 gctgaaggag ctgcgcgcgg ccgcgggcgc cgagcgcgtg caccagttca tcgatatgca
      541 cgacctcggg gacatgctcg tcggcgccgg gttcgcctct ccggtcatgg acatggaaat
      601 gatccaggtc gattacggca ggggcgatgg gcttgtcgcc gacctgcgct cgagcggcca
      661 gacgagcgcg cgtgcggacc gtcgccgcgg gctcggcggg cgcgcaatgc gcgcgcggct
      721 gcgcgcagcg ctgccgcctc gcgtgacgtt cgaagtcgtg tacgggcacg cctggaaggg
      781 gcgcaaggtg ccacccgaag tgaaaagcgt tcgtgttttc aagcgcatgc cttgaggcgc
      841 gagttccggt tgtgatactt tcgctttttc ggtggacacc ccacaattca gccggactct
      901 gaagcgtaat tgcgcgatgt caccggcggg tctggcgagg gtgttcgcgg cgctcgcgct
      961 gctggtgctc gcgatcggcg ccggcttcgc tgtcgcgggc gcgtggctcg ttctgccgtt
     1021 cgcgggactc gaggtcctgt tgctcggcgc ggcattcgtg gcgcatgcgc gccacgcggc
     1081 cgactacgag aagatcgagc tgcactcggg ccgcctcgcg gtcgaagtag cgcaagccga
     1141 gcgcgtcgcg cgctacgagc tcgagaacgc cagggtttcg atcgaagaag ggcgcgtcgt
     1201 gctgcgcgat gcgagaaaag aactcgagat cggccggcat atcggtgccg aagcgcgcgc
     1261 cgagctggcc gccgagctgg aaaagacgct ctttcaggga agaacgacat gaagggaagc
     1321 aagcccgtgc cacgcgcact cgctgttctc gccggattgg ccgccgccgc cgctcccgga
     1381 gcggcgcacg cgatcgcctg gtacttccag acgcccgcct cgaagatggc gcaggacatc
     1441 gacacgctgc accaggtgat catgtggatc atcgtggtca tcttcatcgg cgtgttcacc
     1501 ttcatgttct atgcctgcta tgcgcaccga aagtcgatcg gccacaaggc ggcgcagttt
     1561 cacgagaaca ctaccgtcga gctgctgtgg accatcatcc cggcgctcat cctcatcatc
     1621 atcgcgtggc cggtgaccaa ggtcgtcatc gcgcagaagg acacctcggc ccccgacctg
     1681 accgtcaagg tcaccggcta tcaatggaag tggggctacg actatctgcg cggcgagggc
     1741 gagggcatca gcttcgtgtc caacctcgcc acgccgcgcg atcagatcga gggcaaggca
     1801 cccaaggggg agaactatct cctcgaggtc gacaacgaga tggttgtccc cgtggggaag
     1861 aagatccgcg tcattaccac cgcggccgac gtgatccacg cctggtggat cccggccttc
     1921 ggcgcgaagc aggacgccat ccccgggttc ctgcgcgaca cgtggttccg cgcggagcag
     1981 ccgggcacct accgcggcca gtgcaccgag ctctgcggga aggagcatgc cttcatgccg
     2041 atcgtggtgc gcgcggtgtc cgaggcggac tacacgaagt gggtcgggga gcgcaagaag
     2101 ctcgccggcc cggcccaggc agcggcgccg gtcgcatcgg cggctgcggc ttcggccgct
     2161 cccgcgcctg ccgcttcggc ggcggcgccc gccaagcccg ccgccgacga cagcaagccg
     2221 ctgccgctcg ccgagttggt gagccggggc gaaaaa
//
LOCUS       DASUNT010000345         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_400113, whole genome shotgun sequence.
ACCESSION   DASUNT010000345 DASUNT010000000
VERSION     DASUNT010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400113"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..626
                     /locus_tag="VFX94_06975"
     CDS             63..626
                     /locus_tag="VFX94_06975"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5767961.1"
                     /translation="MCKEPINFEYLVAYWLGELAAGEEEKIEEHLFGCAHCTRRLEWL
                     AALSAGVRAALRAGALGLVVSAPFVEAMKRSGMRLREYRLDPGGRVDCTIRADEDAVV
                     SRIRAPLAGVRRLDALQRVEVGGVEEREVRLEDVPFDPRSGEFIFIPHPAALKKMPAH
                     RFRVRLVSVGEAGEVPVGEYTFEHSPS"
     gene            765..>2416
                     /locus_tag="VFX94_06980"
     CDS             765..>2416
                     /locus_tag="VFX94_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5767962.1"
                     /translation="MKTFLIAALSTLALVQAGCQTSGIQSAPLTEGDDIVGVVIGPSG
                     KPEAGVWVIAETDDLPTKFSKIVVTDEEGRFAVPDLPKANYQLWVRGYGLVDGQKWRT
                     TPGSTVSLLSGREATPGEAAEIYPGMYWFAMLKVPAASEFPLGPVQSQQQWLNTIKSG
                     ACQSCHALGTPGMRRVPEFFSKGRTSHEAWRERLRAGSAQALMARDIGRLDGERAIAN
                     FADWTDRIARGELPFDEPRRPEGIERNLVITMWDYLDPRFYLHDVVSTDRRNPRVNQG
                     GAVYASPEDSTDIVAVLDPRTHSAIDLPHPVRDASTPSVKGNPYGASAYFGDKPYWEG
                     RTLNHNPMMDERARTWFTSRISPDPNPEWCKTGSDHPSAKAYPLNGGANRHLSFYDPW
                     KQEWTLIRTCFPTHHLNFSKDNVLWTSPGVVGPGVIGWLDVKAFERTKDERASQGWTP
                     FILDTNGDGKRGDYVAHDRPLDPKLDKQVMLNHYSVAVSPADGSIWATVLGYPGAIIR
                     VVPGPDPVNTALTEVYEPPLPGYGPRGGDIGADGVFWLSLSSG"
BASE COUNT          413 a          780 c          866 g          357 t
ORIGIN      
        1 tgggacggct gcgggagtgc atgggtcccc gcctacacgg gggtgacaaa tgagcggcgc
       61 caatgtgtaa ggagccgatc aatttcgagt acctcgtcgc ctactggctg ggcgagctcg
      121 cggccggcga ggaggagaag atcgaggagc acctcttcgg ctgcgcgcac tgcacgcgcc
      181 ggctcgagtg gctcgccgcg ctctcggccg gcgtgcgcgc ggcgctgcgc gccggggcgc
      241 tcgggctggt ggtgtcggcg ccgttcgtcg aggcgatgaa gcgctcggga atgcgcctgc
      301 gcgagtaccg gctcgacccg ggcgggcgcg tcgactgcac gatccgcgcg gacgaggacg
      361 cggtggtgag ccgcatccgc gcgccgctcg ccggcgtgcg gcgcctcgac gcgctgcagc
      421 gggtcgaggt aggcggcgtg gaggagcgcg aagtgcgcct cgaggacgtg ccgttcgatc
      481 cgcgctcggg cgagttcatc ttcatcccgc acccggcggc gctcaagaaa atgccggcgc
      541 accggttccg tgtgcggctc gtttccgtcg gcgaggcggg ggaggtgccg gtgggcgagt
      601 acacgttcga gcattcgcct tcctaagcaa tccgggttca gggccgcaat tcgttcccgc
      661 ctacgcggga acagaattgc agccctgacc ccatgcgaaa cggggcgctc tgcctctttt
      721 cctgcgagta gcatgacttc acccacgggg aaagagacat aaaggtgaag acattcctga
      781 ttgcagcgct atccacgttg gcgctcgtgc aggccggatg ccagacgagc ggaatccaat
      841 ccgcaccact caccgaaggc gacgacatcg tcggcgtggt gattggacca agcggcaagc
      901 cggaggcggg cgtgtgggtg atcgcggaga cggacgacct gcccaccaag ttctccaaga
      961 tcgtcgtcac cgacgaggag gggcgcttcg ccgttccgga tctgccgaag gcgaattacc
     1021 agctctgggt gcgcggctac gggctggtgg acgggcagaa gtggcgcacg acgccgggga
     1081 gcacggtgag cctgctcagc gggcgcgagg cgacgcccgg ggaggccgcg gagatctatc
     1141 ccgggatgta ctggttcgcg atgctcaagg tgccggcggc gagcgagttt ccgctcgggc
     1201 cggtgcagag ccagcagcag tggctgaaca ccatcaagag cggcgcgtgc cagtcgtgcc
     1261 acgcgctcgg gacccccggg atgcggcgcg tgcctgaatt tttttcaaaa ggcagaacct
     1321 cgcatgaggc ctggcgcgag cgcctgcgcg cggggagcgc gcaagccctc atggcgcggg
     1381 acatcggccg gctcgacggc gaacgggcga tcgccaactt cgccgactgg accgaccgca
     1441 tcgcaagagg cgagctgccg ttcgacgagc cgcgccggcc cgagggcatc gagcgaaatc
     1501 tcgtcatcac gatgtgggac tacctcgacc cgaggttcta cctgcacgac gtggtgtcga
     1561 ccgaccggag gaatccgcgc gtgaaccagg gcggcgcggt gtacgcctcg cccgaggact
     1621 cgaccgacat cgtcgcggtg ctcgacccgc gcacgcacag cgcgatcgac ctgccgcacc
     1681 cggtgcgcga cgcaagcacg ccgtcggtga agggcaatcc gtacggcgcc tcggcgtact
     1741 tcggcgacaa gccctactgg gagggccgca cgctcaacca caacccgatg atggacgagc
     1801 gcgcgcgcac ctggttcacc tcgcgcatca gccccgatcc gaaccccgag tggtgcaaga
     1861 cgggctcgga ccatccgtcg gcgaaggcgt atccgctgaa cggcggcgcg aaccggcatc
     1921 tctcgttcta cgatccctgg aagcaggagt ggacgctgat ccgcacctgc tttccgacgc
     1981 accacctcaa cttctcgaag gacaacgtgc tgtggacgag cccgggcgtc gtgggccccg
     2041 gcgtgatcgg ctggctcgat gtgaaagcgt tcgagaggac gaaagacgaa agagcgagcc
     2101 aaggctggac gccgttcatc ctcgacacaa atggggatgg aaagcggggc gactacgttg
     2161 cgcatgatcg accgctcgac ccgaagctgg acaagcaggt gatgctcaac cactactcgg
     2221 tggcggtgag cccggccgac gggtcgatct gggcgacggt gctcggctat cccggcgcga
     2281 tcatccgcgt cgtgccgggg ccggacccgg tgaataccgc gctcaccgag gtgtacgagc
     2341 cgccgctccc gggctacggg ccgcgcggcg gcgacatcgg cgccgacggc gtgttctggc
     2401 tctcgctctc gagcgg
//
LOCUS       DASUNT010000346         2727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1101073, whole genome shotgun sequence.
ACCESSION   DASUNT010000346 DASUNT010000000
VERSION     DASUNT010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101073"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..540)
                     /locus_tag="VFX94_06985"
     CDS             complement(148..540)
                     /locus_tag="VFX94_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767963.1"
                     /translation="MKAHLAAIAGVLAAGAAAGASLYSTPVCGCLSPAQAVMGASWPH
                     MDRAAMKTLAGRVPYGRPVAEIHQALAASGYAKYCTDDRAGGKTVCLLPYDRNFWRER
                     AAQVTFAYDAGGIVRSMGAEGKGRYRWE"
     gene            complement(537..887)
                     /locus_tag="VFX94_06990"
     CDS             complement(537..887)
                     /locus_tag="VFX94_06990"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5767964.1"
                     /translation="MIQAVKDDRPLYEVERRAEHAARPGFRITELQISRTQKVPWHYH
                     SNVQDTFYVLEGELRIFLQDPKEEVRLKPGQTYTARPRRPHLVTNGGENSATFLVLQG
                     IGEYDFVPLVEPRK"
     gene            complement(884..1687)
                     /locus_tag="VFX94_06995"
     CDS             complement(884..1687)
                     /locus_tag="VFX94_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5767965.1"
                     /translation="MKLDYVHGYDRRANVRLQDQADALVELLHSDTAYPDGSTVLEAG
                     CGVGAQTVTLARRSPGAHIVSLDIAAASLATARAKVEAAGLANVHFQQADIFALPFER
                     GSFDHVFVCFVLEHLSRPLEALERLKALVKPGGTVTVIEGDHGSTYFHPDSQAAYDAI
                     RCQVELQRAAGGNANIGRELYPLLLRAGLRNVRVSPRMVYVDSSRPRLVEGFTRRTFT
                     AMIEGVRENAIQAGMISPERFDEGIRALYRTAEADGVFCYTFFKGTGEP"
     gene            complement(1696..2340)
                     /locus_tag="VFX94_07000"
     CDS             complement(1696..2340)
                     /locus_tag="VFX94_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5767966.1"
                     /translation="MKLYITPGSPYARMARIVVLEKRLENRVETLIAKTRVPESPYYA
                     VNPSGRVPYLVRDDGVGLEESALICAYLDHLDGRPLLESRSDGAPWEARRLEALARSL
                     LDGLSVWGREILRPENERSPGVIRHETDRAQRMADLWEREIEHPLMGGPLNMIQITLA
                     CALGLEARNPGFRWRAGRPKLSGWFERIATRSSFSATAPPSVQGVLAPFPEKGS"
     gene            complement(2337..>2727)
                     /locus_tag="VFX94_07005"
     CDS             complement(2337..>2727)
                     /locus_tag="VFX94_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX5767967.1"
                     /translation="KLKPFPDVVTALEKLRAAGYKLAILSNGDRDMLRAAGPHIGFRF
                     DHVISVQEAGYFKPQWKTYAKAEEIIAEERSSILFVANHAFDCIGAKSYGMRSAFIDR
                     RKRPFGETPHQPDLIVADFSELAAALT"
BASE COUNT          423 a          905 c          935 g          464 t
ORIGIN      
        1 gcgatgccgc gcggcgcgcc gtcgggtgca tcctttagca cgaggaggaa gttgccgtag
       61 ttcaggccga cgcgaagctt gccattcggg gcaagctcgg atcgcgcggc gggcggtgca
      121 tcgggtgtca tggtagtacg gctccggtca ttcccagcgg tagcggccct ttccctcggc
      181 gcccatcgag cgcacgatcc cgcccgcatc ataggcgaac gtgacttgcg cggcgcgctc
      241 gcgccagaaa ttgcggtcgt acggcagcag gcagaccgtc ttgccgcccg cgcggtcgtc
      301 ggtgcagtac ttggcgtagc cgctcgcggc gagcgcctgg tggatctcgg cgaccggcct
      361 gccgtacggg acgcgcccgg cgagcgtttt catcgccgcg cggtccatgt ggggccagct
      421 cgcgcccatc accgcctgcg cgggcgaaag gcagccgcag accggggtgc tgtagagcga
      481 ggctccggcg gccgcgcctg cggcgagcac tcccgcgatc gcggccagat gcgccttcac
      541 ttgcgtggct cgaccagcgg cacgaagtcg tactcgccga tgccctgcag cacgaggaac
      601 gttgccgagt tttcgccgcc attggtcacc aggtgcgggc gccgcggccg cgcggtgtag
      661 gtctggccgg gcttcaggcg cacttcctct ttcggatcct gcagaaagat cctgagctcg
      721 ccttcgagca cgtagaaagt gtcctgcacg ttgctgtggt aatgccaggg caccttctgc
      781 gtcctcgaga tctgcagctc ggtgatgcgg aagccggggc gcgcggcgtg ctcggcgcgg
      841 cgctcgactt cgtagagcgg acggtcgtcc ttcacggcct ggatcatggc tcgcctgttc
      901 ctttgaaaaa cgtatagcag aaaacgccgt cggcctcggc ggtgcgatag agcgcgcgga
      961 tgccttcgtc gaagcgctcg ggcgagatca tgcctgcctg gatcgcgttc tcgcgcacgc
     1021 cctcgatcat cgcggtgaac gtcctgcgcg tgaagccctc gacgaggcgc gggcggctcg
     1081 agtccacgta caccatgcgc ggcgacacgc gcacgttgcg cagtcccgcg cgcagcagca
     1141 gcgggtagag ctcgcggccg atgttggcgt tgccgccggc ggcgcgctgc agctcgacct
     1201 ggcagcggat cgcgtcgtag gccgcctggc tgtcgggatg gaaatacgtc gagccgtggt
     1261 cgccctcgat cacggtcacg gtgccgcccg gcttcaccag cgctttcagg cgctccagcg
     1321 cctcgagcgg ccgcgataaa tgctcgagca cgaagcacac gaacacgtgg tcgaacgacc
     1381 cgcgttcgaa gggcagggcg aagatgtcgg cctgctggaa gtgcacgttg gcgaggccgg
     1441 ccgcctcgac tttcgctctt gccgtggcga gcgaggcggc agcgatgtcg agcgacacga
     1501 tgtgcgcgcc cgggctgcgg cgcgcgagcg tcacggtctg cgcgccgaca ccgcatcccg
     1561 cctcgagcac cgtgctcccg tcggggtagg cggtgtccga gtgcagcagc tccacgagcg
     1621 cgtcggcctg gtcctgcagc cgcacgttcg cacgccggtc atacccgtgg acgtagtcca
     1681 gtttcataga aggggtcagg accctttttc tgggaaaggt gccaggactc cttgcacgct
     1741 cggcggcgcg gtcgccgaga acgacgaccg cgtggcgatg cgctcgaacc atcccgagag
     1801 cttcgggcgg ccggcgcgcc atcggaaacc gggattgcgc gcctcgaggc cgagtgcgca
     1861 cgcgagcgtg atctgtatca tgttgagcgg cccgcccatg agcggatgct cgatttcccg
     1921 ctcccagagg tccgccatgc gctgggcccg atcggtctcg tgccggatga cgcccggcga
     1981 gcgctcgttc tccggccgca ggatctcgcg cccccagacc gaaaggccgt cgagcaggct
     2041 cctcgccaac gcttcgagac gccgcgcttc ccacggcgcg ccatcgctcc gcgattcaag
     2101 cagcggcctg ccgtccaggt gatcgagata cgcgcagatg agcgccgatt cctccaatcc
     2161 gacaccatcg tcgcgcacga gataaggaac ccgacccgat gggttgacgg cgtagtaagg
     2221 actttcgggg acacgcgttt tcgcgatgag cgtctctacg cggttctcca gtcgcttctc
     2281 caggacgacg atccgcgcca tgcgcgcgta aggggacccg ggagtgatat agagcttcat
     2341 gtgagcgcgg cggcgagctc gctgaaatcg gccacgatga gatcgggctg gtgcggtgtc
     2401 tcgccgaacg ggcgcttacg ccggtcgata aacgccgagc gcatgccgta ggacttcgcg
     2461 ccgatgcagt cgaaagcgtg gttggcgacg aacaggatgc tcgagcgctc ctcggcgatg
     2521 atctcttccg ccttcgcata ggtcttccac tgcggcttga agtaccccgc ctcctggacc
     2581 gagattacgt ggtcgaaccg gaagccgata tgcggcccgg cggctctcag catgtcgcgg
     2641 tcgccgttcg aaagaatcgc gagcttgtat cccgccgcgc ggagcttctc gagcgccgtc
     2701 accacgtcgg gaaagggctt gagcttc
//
LOCUS       DASUNT010000347         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_662603, whole genome shotgun sequence.
ACCESSION   DASUNT010000347 DASUNT010000000
VERSION     DASUNT010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662603"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFX94_07010"
     CDS             <1..292
                     /locus_tag="VFX94_07010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEX5767968.1"
                     /translation="QIPQVHPLIALPDYRATSDAARILLSPRAALKLSKAAEGSSAFV
                     VAAGPEAGFTTEEEAALVSAGFVPTSLGPRVLRTETAAVAALAALNALRGDS"
     gene            complement(267..845)
                     /locus_tag="VFX94_07015"
     CDS             complement(267..845)
                     /locus_tag="VFX94_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767969.1"
                     /translation="MKTIRLLLIPLALVTLAPVQAAEDKAESPALGHALTLIHLFVRI
                     AAESETPQQSLKAVDDVLLGRNPVANEAAAGLMKEMLAEVPPQHRATIVAIGRDLTSL
                     ARKDLKKQAEIDPMRARRDLTSLGLTYHDSKQFLEAVKRNDAIAAELFIAGRGVDLSA
                     RDFWGRDALDFARANGNERLAELIARNRPAER"
     gene            925..2235
                     /gene="argA"
                     /locus_tag="VFX94_07020"
     CDS             925..2235
                     /gene="argA"
                     /locus_tag="VFX94_07020"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEX5767970.1"
                     /translation="MDSSGSFVRWFRQVAPYVHDFGGRTFVVGFGGEMVAERARFESF
                     IHDVNVLASLEIRLVLVHGARPQIEAEMKAKGLRSRYAQGLRITDGQALTAVKHAAGI
                     LRLEMEALLSQGLPNSPMARAQIRVVSGNYITARPIGVRNGVDFQFTGTVRKVDAAAL
                     SAHLDVADIVLVPHLGYSATGDVFNLAWEDVAESVAVALRADKLLMYTDRLPADRRDE
                     VISELTADEATSVARKGDLTPAAKRALEHLARAVEGGVARGHLVTRRAPGSLLLELFT
                     HAGVGTMVTADTTEQLRPAKIEDVRGMLALIEPLEAEGTLVKRSRELLEAEIGNFVVV
                     EHDARIVGCAALYPFPDDKSGEFACLAVASEYRDAGYGERLLKACEERAKKMRLRKLF
                     ALTTHAAHWFLEQGFRAAPVDALPDGRKALYNWRRGSKVFLKRI"
     gene            2243..2515
                     /locus_tag="VFX94_07025"
     CDS             2243..2515
                     /locus_tag="VFX94_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEX5767971.1"
                     /translation="MARMVKCVKLGREAEGLDFPPYPGALGKRIWENVSKEAWAQWLK
                     HQTMLVNENRLNLADPRARKYLMEQTERHFFGVGADVASGYVPPER"
     gene            complement(2494..>3269)
                     /locus_tag="VFX94_07030"
     CDS             complement(2494..>3269)
                     /locus_tag="VFX94_07030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5767972.1"
                     /translation="LLARFLVGQMAGVAFGTAAAGWLGEHFGWRSIFWALGALLLAIA
                     LLLFLELQNPLVGTARRSSIRESLKRMPRLLLQRPLQVLLATGYTEGALIFGALAFVA
                     IHLQRRYGIGPGLAGTLVTAYAAGGLLYALLARRAHRRFGERGLATLGGLALVFGYAG
                     LAFATSPTRATLCIACVGVGFYMLHTTVQTHVTHVKPEERGSTVALFATFLFLGQASG
                     VWLGAQIVDVAGLAPVFVAAAVGLAVLTVSFRYYLSGGT"
BASE COUNT          584 a         1077 c         1110 g          498 t
ORIGIN      
        1 ccaaatcccg caagtccatc cgttgatcgc gctcccggat taccgggcaa ccagcgacgc
       61 cgcgcggatc ctgctttctc ctcgggcggc gctcaagctt tccaaggcag cggaaggctc
      121 aagcgctttc gttgtggcgg cgggcccgga ggcagggttc accacggaag aggaagcggc
      181 gctcgtgtcg gccggcttcg tgcccacttc actcgggccc cgggtgctgc gcaccgagac
      241 agcggccgtg gccgcgcttg ccgcgctcaa cgctctgcgg ggcgattcct agcgatcagc
      301 tcggcgaggc gctcgttgcc gttggcgcgg gcgaagtcga gcgcgtcgcg accccagaag
      361 tcgcgcgccg aaaggtccac gccgcgcccg gcgatgaaga gctcggccgc gatggcgtcg
      421 ttgcgcttca ccgcctcgag gaactgcttc gaatcgtggt acgtgaggcc cagcgaggtg
      481 aggtccctgc gcgctcgcat gggatcgatc tcggcttgct tcttcagatc ctttctggcc
      541 aacgaagtca ggtctcgccc gatggcgaca atggtggcgc gatgctgcgg cggcacttcg
      601 gcgagcatct ccttcatgag acccgccgcg gcctcgttcg ccacggggtt tcgcccaagc
      661 agcacatcgt cgaccgcctt aaggctctgc tgcggcgtct ccgattccgc ggcaatgcgt
      721 acgaaaaggt ggatcagcgt gagcgcatga ccgagcgccg gcgactcggc cttgtcctcg
      781 gccgcctgca cgggggcgag cgtgacgagc gccaaaggga tgagcagcaa gcggattgtt
      841 ttcatcgatc gttcctccgg atggcgaaag gatagcgcaa gcctgcagca tttccggcag
      901 tcagtagaat tgaaaaaatt acacgtggat agctcgggaa gcttcgttcg gtggttccgc
      961 caggtcgcgc cctatgtgca cgacttcggc gggcgcacgt tcgtggtcgg cttcggcggc
     1021 gagatggtcg ccgagcgcgc gcgcttcgag agcttcatcc acgacgtgaa cgtgctcgcc
     1081 tcgctcgaga tccggctcgt gctggtgcac ggggcgcggc cgcaaatcga agccgaaatg
     1141 aaagcgaagg ggctgcgctc gcgctacgcg cagggcctgc gcatcaccga cgggcaggcg
     1201 ctcaccgcgg tcaagcacgc cgccggcata ctgcgcctgg aaatggaggc gctgctctcg
     1261 caagggctgc cgaattcgcc gatggcgcgc gcacagatcc gcgtcgtctc gggcaactac
     1321 atcacggcac ggccgatcgg cgtgcgcaac ggcgtcgatt tccagttcac cggcacagtg
     1381 cgcaaggtcg acgcggccgc tctctccgct cacctcgatg tcgcggacat cgtgctcgtg
     1441 ccgcatctgg gctattcggc gaccggcgat gtattcaacc tggcgtggga ggacgtcgcg
     1501 gaaagcgtcg cagtggcgct gcgtgcggac aagctcctca tgtacaccga ccggctcccc
     1561 gccgaccgca gggacgaggt gatttccgag ctcaccgccg acgaggcaac atcggttgcc
     1621 aggaagggcg atctgacgcc tgcggcgaag cgagcgctcg agcacctggc gcgcgcggtc
     1681 gaaggcggcg tggcgcgcgg gcacctcgtc acgcggcgcg cgccgggatc gctgctactg
     1741 gagctcttca cccatgcagg cgtcggcacc atggttaccg ccgatacgac cgagcagttg
     1801 cgcccggcga agatcgagga cgtgcgcggc atgctggctc tgatcgagcc gctcgaagcc
     1861 gaaggcacgc tggtgaagcg aagccgcgag ctgctggagg ccgagatcgg caatttcgtg
     1921 gtggtagagc acgacgcccg aatcgtcggc tgtgcggcgc tctatccctt tcccgacgac
     1981 aagagcggcg agttcgcctg ccttgccgtg gcgagcgaat accgcgacgc cggctacggc
     2041 gagcggctgc tcaaggcgtg cgaggagcgc gcgaaaaaaa tgcggctgcg caagctcttc
     2101 gccctcacta cgcacgcggc gcactggttc ctcgagcagg gcttccgcgc cgcgccggtc
     2161 gacgcgctgc ccgacgggcg aaaggcgctc tacaactggc gccgcggttc taaggtcttc
     2221 ctcaagcgaa tataatctcg cgatggccag aatggtgaag tgcgtgaagc tcggccgcga
     2281 ggccgagggg ctggatttcc ccccgtatcc cggcgctctc ggcaagcgga tctgggagaa
     2341 cgtctcgaag gaagcatggg cgcagtggct caagcaccag acgatgctgg tgaacgagaa
     2401 ccgcctgaac ctcgccgacc cgcgcgctcg caaatacctc atggagcaga ccgagcgcca
     2461 tttcttcggt gtgggcgccg acgtggcgtc gggctacgtc ccacccgaga ggtagtagcg
     2521 gaaagaaacc gtcaggaccg cgagcccgac cgccgcggca acgaaaaccg gggcgagccc
     2581 cgccacatcg acgatctgcg cgccaagcca cacgccggag gcctgcccca ggaagagaaa
     2641 agtcgcgaaa agcgcgaccg tcgatccgcg ctcttccggc ttgacgtggg tgacgtgcgt
     2701 ctgcaccgtc gtgtgcagca tgtagaagcc gactcccacg caagcgatgc agagcgtcgc
     2761 tcgcgtcggt gacgtcgcga atgcgagtcc ggcatagccg aacaccagcg cgagccctcc
     2821 caacgtggca aggccgcgct cgccgaagcg ccggtgcgcc cggcgcgcga gcagcgcgta
     2881 gagcagccct cccgcagcgt aggccgtgac cagcgtcccg gcgagcccgg gcccgatgcc
     2941 gtatctgcgc tgtaaatgga tggcgacgaa tgcgagcgcg ccaaaaatca gggcgccttc
     3001 ggtgtacccg gtcgcaagga gcacctgcag cgggcgctgc aaaagcagcc gcggcatgcg
     3061 tttcaggctc tcgcggatcg agctgcggcg cgcggttccc accagcgggt tctgcaattc
     3121 caggaaaagc aatagcgcaa tggcaagcag cagcgcgccg agcgcccaga aaatcgagcg
     3181 ccagccgaaa tgctcgccga gccatccggc agccgcggtg ccgaacgcca cgccggccat
     3241 ctggccgacg aggaagcgcg cgagcagcg
//
LOCUS       DASUNT010000348         2227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1013025, whole genome shotgun sequence.
ACCESSION   DASUNT010000348 DASUNT010000000
VERSION     DASUNT010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013025"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VFX94_07035"
     CDS             complement(<1..474)
                     /locus_tag="VFX94_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEX5767973.1"
                     /translation="MLERKPLPERFCNLERLLAAMAARGLDAIVASTSLNVFYLSGFN
                     GIAHKADEPHPYALMLSRHAPEHPILVIADYYLATFLAQPSWVRDIRPFRAVMMPIDR
                     APKREDIDHFVPRAAHDVVWVRRAREHYAFDMATALRSALVELGLERGKVGFDDLG"
     gene            complement(474..1640)
                     /locus_tag="VFX94_07040"
     CDS             complement(474..1640)
                     /locus_tag="VFX94_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX5767974.1"
                     /translation="MTTGSPLAARGAVRALTASKIRELYNAGIGNPNVLPFWVGEPDE
                     PTPDFIRKAGTESIAAGEVFYTHNLGIPELREALAAYISRLHRETQTEQIAVTSAGVN
                     ALMVASQLLVDAGDRVVQVVPLWPNLQEIPKILGASVDTVALRFSQKGWTLDLDELLD
                     RLKPGTRALYLNSPNNPTGWTVTKPELEAILERCRCHGIWIFADDAYERLYYGEEGIA
                     PSFLDISSDEDRLISANTFSKSWLMTGWRLGWLVVPPELTADLGKLIEYNTSCAPVFV
                     QRAGIAAVKEGEPVIARTRYRFRKARDFLVSELKEIPKVKVAVPSGTMYAFFRVEGVA
                     DSLAFCKQLVRDVGLGLAPGSAFGPEGEGFVRWCFAASEEKLAEGVARLKRALQ"
     gene            1639..>2227
                     /locus_tag="VFX94_07045"
     CDS             1639..>2227
                     /locus_tag="VFX94_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5767975.1"
                     /translation="MNRRILDWSLVAAVGTMVAWGMNFAFVKYVLDHLGIGAFMFLRF
                     AVLPLLGLVLLVAIFRHRVAHTWPRRADLPRFLLCALVGHVLHISAVMYGMNLSTAFS
                     SSLVLTCQPLFTLAILAALGAERLRRQQVAGTLIAFSGIVLFLADKFAAGLSRAGLGD
                     LTLLVAGAAFSAYTVLQRPLADRYGPLIVLSYTLLF"
BASE COUNT          353 a          753 c          710 g          411 t
ORIGIN      
        1 gccgaggtcg tcgaagccta ccttgccgcg ctccaggcca agctccacca gcgcgctgcg
       61 cagtgcggtc gccatgtcga acgcgtagtg ctcgcgcgcg cggcgcaccc atacgacatc
      121 gtgcgcagcg cgaggaacga aatgatcgat gtcttcgcgc ttcggggcgc ggtcgatcgg
      181 catcatcacc gcgcggaacg gccggatatc gcgtacccag ctcggctggg cgagaaaggt
      241 cgcgaggtaa tagtccgcga tcaccaggat cggatgctcg ggcgcgtgcc gcgagagcat
      301 gagcgcgtac ggatgcggct cgtccgcctt gtgcgcgatg ccgttgaagc cgctgaggta
      361 gaacacattg agcgacgtcg aggcgacgat ggcgtccagc ccccgcgccg ccatcgcggc
      421 cagcagccgc tcgaggttgc agaagcgctc tggaagcggt tttcgttcaa gcatcactgc
      481 aacgcccgct tcaggcgggc aaccccctcc gccagcttct cttccgacgc ggcgaagcac
      541 cagcgcacga agccttcgcc ttcgggcccg aacgccgagc cgggcgcgag gccgaggccg
      601 acatcgcgca ccagctgctt gcagaaggcg aggctgtccg ccacgccttc gactctaaag
      661 aaggcgtaca tggtgccgga gggcacggcg actttcactt tcggaatctc tttcagctcg
      721 gaaacgagaa aatcccgcgc cttcctgaaa cggtagcgcg tgcgcgcgat caccggctcg
      781 ccctccttca ccgcggcgat accggcgcgt tgcacgaaga ccggggcgca ggacgtgttg
      841 tattcgatca gcttgccgag atccgcggtg agctccggcg gcacaacgag ccagccgagc
      901 cgccagccgg tcatgagcca cgacttcgag aacgtgttgg cgctgatgag ccggtcttca
      961 tccgaggaaa tatcgagaaa agaaggcgcg atgccctcct cgccgtagta caggcgctcg
     1021 tacgcgtcgt cggcgaagat ccagatgccg tggcagcggc agcgctcgag gatggcctcc
     1081 agctcgggct tggtgacggt ccacccggtc ggattgttgg gcgaattcag atagagggct
     1141 cgcgtgcccg gcttgagccg gtcgagcaat tcatccaggt cgagcgtcca tcccttctgc
     1201 gagaacctca acgccacggt gtccaccgag gcgcccagga tcttcgggat ctcttgcagg
     1261 ttcggccaca agggaactac ctgaaccacg cggtcgccgg catcgacgag cagctgggag
     1321 gcgaccatca gtgcgttcac gccggcgctg gtcaccgcga tttgctcggt ttgagtttct
     1381 ctatgtaacc gagaaatgta ggctgcgagc gcctctctca gctccggaat gccgaggttg
     1441 tgcgtataga acacctcgcc cgcggcaatc gactcggtcc ctgccttgcg gataaagtcc
     1501 ggcgtcggct cgtcgggctc gcccacccag aacggcagca cgttggggtt gccgatgccg
     1561 gcgttgtaga gttcgcggat tttggacgcg gtcagcgcgc gcaccgcgcc tcgcgccgcc
     1621 agcggagatc cggttgtcat gaaccgccgc attttagact ggtcgctcgt cgcagccgtc
     1681 gggacgatgg tcgcctgggg catgaacttc gcgttcgtga agtacgtgct cgaccacctg
     1741 gggataggcg ccttcatgtt cctgcgcttc gcggtgctgc cgctcctcgg cctggtgctc
     1801 ctcgtagcga tcttccggca tcgcgtcgcg catacctggc caaggcgcgc cgacctgccg
     1861 cgcttcctcc tgtgcgcgct ggtcggccac gtgctgcata tcagcgcggt gatgtacggg
     1921 atgaacctct ccaccgcgtt ctcctcgtcg ctggtgctca cctgccagcc gctcttcacg
     1981 ctcgccatcc ttgccgcgct tggcgccgag cgcctgcgcc gccagcaggt cgccgggacg
     2041 ctgattgcgt tctccggcat cgtcctcttc ctcgccgaca agttcgccgc cggcctctcg
     2101 cgcgccggcc tgggcgacct gacgcttctc gtcgcgggcg cggcgttttc cgcctatacc
     2161 gtgctccagc ggccgctcgc cgatcgctac gggccgctca tcgtgctctc gtatacgctg
     2221 cttttct
//
LOCUS       DASUNT010000349         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_838445, whole genome shotgun sequence.
ACCESSION   DASUNT010000349 DASUNT010000000
VERSION     DASUNT010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838445"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1823
                     /locus_tag="VFX94_07050"
     CDS             <1..1823
                     /locus_tag="VFX94_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX5767976.1"
                     /translation="KHFLQTLIAVEDRQFYAHAGFDVRGLARAARSLFTGRVQGGSTI
                     TQQLVKNFFLTPERTIERKLTELVMAVLLELHYDKDEILETYLNEIYFGQDRDRAIHG
                     VGLAAQFYFGKEVQHLNPAESALLVGMIRGPAHYDPHRHAQRALERRNFVLREVRDEK
                     GITAEEYAQARASPLNVSKKAGMGTTPYPAFLQLVHRQLRRDYDEADLRTEGLRIFTT
                     LDPRVQEAAEAALTKRLGQFDKERRFGREPGLEGAVVVSDPQSGELHALVGGRDPRYR
                     GYNRALDASRPVGSLLKPAIYLTALADSARYTLITPIDDGPFVWKSRGAPDWQPANYD
                     KKFHGLVPLRTALAQSYNAAAARLGTEIGIERVLDTVRRLGVDRPMRPFASTLLGAIE
                     LSPLEVAQMYQTIASGGFRSPLRAIREVTTQEGRPLTRYPLAVEQVFAPEPIYLLAAA
                     MQDVVREGTAQSLSKFLPSDIAVAGKTGTTDEQRDAWFAGFTGDRLAIVWVGYDDNRA
                     ARLSGSTAALPIWGDMMAALAPEPLALAKPEGIELVLIDPQTGLRGGTSCPGATEVPF
                     MQGSAPQERAPCSGTMDAAVEAVEQTVQKAKSWLERLFGR"
     gene            1835..2254
                     /locus_tag="VFX94_07055"
     CDS             1835..2254
                     /locus_tag="VFX94_07055"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5767977.1"
                     /translation="MILVLLGGCATPPAPPETRPEQPPPVAPAPPPAKENIAVAGLME
                     TARADAASGNLAGAAASIERALRIEPRNARLWHELARVRLKQGQYAQAENVAARSNSW
                     TGDDRALRAENWRLIAESRRARGDADGAKAALDAAAR"
BASE COUNT          368 a          812 c          768 g          314 t
ORIGIN      
        1 cgaagcattt cctccagacg ctgatcgccg tcgaggaccg ccagttctac gcgcacgccg
       61 ggttcgacgt gcgcgggctc gcgcgcgcgg cgcgcagcct cttcaccggc cgggtgcagg
      121 gcggaagcac gatcacccag cagctcgtca agaacttctt cctcacgccg gagcgcacta
      181 tcgagcgcaa gctcaccgag ctcgtcatgg cggtgctgct cgagctgcac tacgacaagg
      241 acgagatcct cgaaacctac ctcaacgaga tctacttcgg gcaggaccgc gaccgcgcca
      301 tccacggcgt cgggctcgcc gcgcagttct acttcggcaa ggaggtgcag cacctgaacc
      361 ccgccgagtc agcgctcctt gtcggcatga tccgcgggcc ggcgcactac gacccgcacc
      421 gccacgccca gcgcgcgctc gagcggcgca acttcgtgct gcgcgaggtg cgcgacgaga
      481 aaggcatcac cgccgaggag tacgcgcagg cacgcgcgag cccgctcaac gttagcaaga
      541 aagccggcat ggggacgacg ccgtatccgg cctttctgca gctcgtgcac cggcagctgc
      601 gccgcgacta cgacgaggcc gatctgcgca cggaggggct gcggattttc accacgctcg
      661 atccgcgggt gcaggaagcg gccgaagctg cgctgaccaa gcgcctcggg cagttcgaca
      721 aggagcggcg cttcgggcgc gagcctggtc tcgagggcgc cgtcgtggtc agcgatccgc
      781 agagcggcga gctccacgcc ttggtcggcg gccgcgatcc gcggtaccgc ggctacaacc
      841 gggctctcga cgcctcgcgc ccggtcggct cgctgctcaa gcccgccatc tacctcacgg
      901 cgctcgccga ttctgctcgt tacacgttga tcacccctat agacgacggg ccgttcgtgt
      961 ggaagagccg aggcgccccc gactggcaac cggcgaacta cgacaagaag tttcacggcc
     1021 tcgtgccgct acgcaccgcg ctcgcgcagt cctacaacgc cgcggccgct cgcctgggca
     1081 ccgagatcgg gatcgagcgc gtgctggaca cggtaaggcg gctcggcgtg gaccggccga
     1141 tgcggccgtt cgcttcgacc cttctgggcg caatagaact gagtccgctc gaggtcgcgc
     1201 agatgtacca gacgatcgca agcggcgggt tccgctcccc gctgcgcgcg atccgcgagg
     1261 tcacgacgca ggaaggacgt ccgctcacgc gttacccgct tgcggtcgag caggtgttcg
     1321 cgcccgagcc gatctacctc ctcgccgcag ccatgcagga cgtggtgcgc gaaggaacgg
     1381 cgcagagcct gagcaagttc ctgccgtccg acatcgcggt cgcgggcaag acgggcacca
     1441 cggacgagca gcgcgacgcc tggttcgccg gcttcaccgg cgaccggctc gccatcgtct
     1501 gggtgggata cgacgacaac cgcgcggcgc gcctctcggg ttcgaccgct gcgctgccga
     1561 tctggggcga catgatggcg gcgctcgcgc ccgagccgct cgcgctggcg aagcccgagg
     1621 gcatcgagct cgtcttgatc gacccgcaga ccggcttgcg cggcggcacg agctgccccg
     1681 gcgccaccga ggtgcctttc atgcagggct ccgcgccgca ggagcgcgcg ccctgctccg
     1741 gcaccatgga tgccgcggtt gaggccgtag agcagacggt gcaaaaggcg aagagctggc
     1801 tcgagcggct gttcggacga tgagaaagcg cttattgatt ctcgtcttgc ttgggggctg
     1861 cgcaacgcca cccgccccgc ccgagacgcg tcccgagcag ccgccgccgg tcgctccggc
     1921 gccgccgccc gccaaggaga acatcgcggt cgcgggcctc atggagacgg cgcgcgccga
     1981 tgccgcgtcg ggcaacctcg ccggcgccgc agcgtctatc gagcgcgcgc ttcgcatcga
     2041 gccgcgcaac gcgcgcctgt ggcacgagct cgcgcgcgtg cgcctgaaac aggggcagta
     2101 tgcgcaggcc gaaaacgtcg ccgcgcgctc gaactcctgg acgggcgacg accgcgcgct
     2161 gcgcgcggaa aactggcggt tgatcgccga atcgcgccgt gcacgcgggg acgcggacgg
     2221 cgccaaagcc gccctcgacg ctgccgcccg ctagaattag tc
//
LOCUS       DASUNT010000350         2711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_488023, whole genome shotgun sequence.
ACCESSION   DASUNT010000350 DASUNT010000000
VERSION     DASUNT010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488023"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFX94_07060"
     CDS             complement(<1..542)
                     /locus_tag="VFX94_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767978.1"
                     /translation="MKPIRTLLIAILLIPALPAHAAGEESPALGHALTLIHLFVRIAA
                     ESETPQQSLKAVDDVLLGRNAAANEAAAGLMKEMLADMPPQHRATIVAIGRDLTSLAR
                     KDLRKQGAIDPLQARRDLTSMGLTYHDSKQFLDAVKRNDAIAAELFIAGRGVDLSARD
                     FWGRDAVDFARASGNERLAEL"
     gene            612..1928
                     /gene="argA"
                     /locus_tag="VFX94_07065"
     CDS             612..1928
                     /gene="argA"
                     /locus_tag="VFX94_07065"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEX5767979.1"
                     /translation="MDSSGNFVHWFRQVAPYVHDFGGRTFVIAFGGEMVAERARFQSF
                     IHDVNVLASLEIRLVLVHGARPQIEAEMKSKGLRSRYAQGLRITDEQALTVVKHGAGI
                     LRLEIEALLSQGLPDSPMARAQIRVVSGNYITARPIGVRNGVDFQFTGAVRKVDAAAL
                     AAHLDVADIVLVPHLGYSATGEVFNLAWEDVAESVAVALRADKLLMYTDRLPADRKGG
                     LISELTADEAAALAGKKAAGLTPAAARALEHVARAVEGGVTRGHLVTRRAPGSLLLEL
                     FTHAGVGTMITADAVEKLRPAKIEDVRGMVALIEPLEADGTLVKRSRELLEAEIGNFL
                     VVEHDTRIVGCAALYPFPDDKSGEFACLAVAAEYRDAGYGERLLKACEERAKSLRLRK
                     LFALTTHAAHWFLEQGFRASPVEALPEKRKALYNWRRGSKVFLKRI"
     gene            1936..2208
                     /locus_tag="VFX94_07070"
     CDS             1936..2208
                     /locus_tag="VFX94_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEX5767980.1"
                     /translation="MARMVKCVKLGREAEGLDFPPYPGALGKRIWENVSKEAWAQWLK
                     HQTMLVNENRLNLADPRARKYLTEQTERHFFGAGADVASGYVPPDK"
     gene            complement(2187..>2711)
                     /locus_tag="VFX94_07075"
     CDS             complement(2187..>2711)
                     /locus_tag="VFX94_07075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5767981.1"
                     /translation="LATGYAEGVFIFGALAFVAIYLQRRYGIGPGLAGTLVTAYAAGG
                     LVYALLARRALRRFGERGLATLGGLALVIGYGGLAFATSSPLATLCIAGVGVGFYMLH
                     TTVQTHVTHVAPQERGSAVALFATFLFLGQASGVWLGAQIVDTLGLAPVFAAAAVGLC
                     ALTALFRFYLSGGT"
BASE COUNT          491 a          919 c          908 g          393 t
ORIGIN      
        1 agctcggcga ggcgctcgtt gccgctggcg cgagcgaaat ccaccgcatc gcggccccag
       61 aaatcgcgcg ccgaaagatc gacgccgcgc ccggcaatga agagctcggc ggcaatggcg
      121 tcgttacgtt tcaccgcatc gaggaactgc ttcgagtcgt ggtacgtgag ccccattgac
      181 gtgagatccc tgcgcgcctg cagcggatcg atggcgccct gcttgcgcag atccttcctc
      241 gcgagcgagg tcagatcgcg cccaatcgcg acgatggttg cgcgatgctg aggcggcatg
      301 tcggcaagca tctccttcat taggcccgcg gcagcctcgt tcgccgccgc attgcgcccg
      361 agcagcacgt cgtcgaccgc cttcaggctc tgctgcggcg tctcggattc cgcagcgatg
      421 cgcacgaaca gatgaatcag cgtgagcgcg tggccgaggg ccggcgactc ctcgccggcg
      481 gcgtgcgccg gcaaagccgg gatgagcaga atagcgatga gcagcgtgcg gatgggtttc
      541 accgggtcgt cctccgaagc gctgagcata gcgcagcctg caggccccgg tagaatttga
      601 aagaactaca catggatagc tcggggaact tcgttcactg gttccggcag gtcgcgccgt
      661 acgtgcacga cttcggcgga cgcacgttcg tgatcgcctt cggcggcgag atggtcgccg
      721 agcgtgcgcg cttccaaagc ttcatccacg acgtcaacgt gcttgcttcg ctcgagatcc
      781 gcctcgtgct ggtgcacggc gcgcggcccc agatcgaggc cgaaatgaag agcaagggac
      841 tgcgctcgcg ctacgcgcag ggcttgcgca tcaccgacga gcaggccctc accgtggtaa
      901 agcacggggc cggcatactg cggctggaaa tcgaggcact gctctcgcag gggctgcccg
      961 actcgcccat ggcgcgcgcc cagatccgcg tcgtctcggg aaattacatc accgcgaggc
     1021 cgatcggcgt gcgcaacggc gtggattttc agttcaccgg cgcggtgcgc aaggtggacg
     1081 cggcggcgct tgccgctcat ctcgacgtcg cggacatcgt gctcgtgccg cacctcggct
     1141 attcggccac cggggaagtc ttcaacctcg cctgggagga cgtcgccgaa agcgtcgcgg
     1201 tggcgctgcg cgccgacaag ctcctcatgt acaccgaccg gctgcccgcc gaccgcaaag
     1261 gcgggctcat ctccgagctc accgcggacg aggcggccgc gctcgccggc aagaaagccg
     1321 ccgggctcac gccggccgcg gcacgcgcgc tcgagcacgt tgcgcgcgcg gtcgagggcg
     1381 gcgtgacgcg cgggcacctc gtgacgcggc gggcgccagg gtcgctcctg ctcgagcttt
     1441 tcacccacgc cggcgtcggc accatgatta ccgccgacgc ggtcgagaag ctgcgcccgg
     1501 cgaaaatcga agacgtgcgc ggcatggtgg ccctgatcga gccgctcgag gccgacggta
     1561 ccctggtgaa gcgaagccgc gagctgctcg aggccgagat cggcaacttc ctggtcgtgg
     1621 agcacgacac gcgcatcgtc ggctgcgcgg cgctttatcc ctttccggac gacaagagcg
     1681 gcgagttcgc ctgcctcgcg gtcgcagccg aatatcgcga tgccggctac ggcgagcggc
     1741 tgctcaaggc ctgcgaggaa cgcgcgaaga gcctgcggct gcggaaactc ttcgccctca
     1801 ccacgcacgc ggcgcactgg ttcctcgaac agggcttccg cgcctccccg gtcgaggcgc
     1861 tgcccgagaa acgcaaggcg ctctacaact ggcgtcgcgg ctcgaaggtg tttctcaaaa
     1921 gaatataatt tcgtaatggc gagaatggtg aagtgcgtaa agctcgggcg cgaagccgag
     1981 gggctcgatt tcccacccta ccccggcgcg ctcggcaagc gcatctggga gaacgtgtcc
     2041 aaggaagcgt gggcgcagtg gctcaagcac cagacgatgc tcgtaaacga gaaccgcctc
     2101 aacctcgccg acccccgcgc gcgcaagtac ctcacggagc agaccgagcg tcacttcttc
     2161 ggcgccggcg ccgacgtcgc ctcgggctac gttccgcccg acaagtagaa gcggaagagc
     2221 gccgtcagag cgcagagccc gacggctgca gccgcgaaaa ccggcgccag cccgagggta
     2281 tcgacgatct gcgcgccaag ccacacgccc gaagcctgcc cgagaaagag aaacgtcgcg
     2341 aaaagcgcga ccgccgagcc gcgctcttgc ggcgcgacgt gcgtaacgtg cgtctgcacg
     2401 gtcgtgtgca gcatgtagaa gccgactccc acgcctgcaa tgcacagagt cgcgagcggt
     2461 gaggacgtgg cgaatgcaag tccgccataa ccgatcacca gggccaggcc tccaagcgtt
     2521 gcgaggcctc gctccccgaa ccgccggagc gcgcggcgcg cgagcagcgc atagacgagt
     2581 ccgccggcgg cgtaggccgt cacgagcgtc ccggcaagcc cgggcccgat gccgtagcgc
     2641 cgctgcaagt aaatcgcgac gaacgcgagc gcgccgaaga tgaacacgcc ctccgcgtac
     2701 ccggtggcaa g
//
LOCUS       DASUNT010000351         2538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_488196, whole genome shotgun sequence.
ACCESSION   DASUNT010000351 DASUNT010000000
VERSION     DASUNT010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488196"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1567
                     /locus_tag="VFX94_07080"
     CDS             164..1567
                     /locus_tag="VFX94_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5767982.1"
                     /translation="MPLLLIFAVLLAVALTGCSTPFVEPEVEVAGRFAQADASLDEPE
                     VAWWESYGDAVLSSLIRRAAQQNRDIKMAATRVRAARAGETISRSRMLPNVGVTAAGN
                     YQRTEYDAAAKQEVPDVKSLSGGIDVSWEIDLSGRLRSATSAAAAEALAAEHGERGVR
                     LLVLTDVATNYFVLVGASRQLETVRAIALAHDETLRLVTARQRVGLATPFDVERAQTE
                     ASRANAAIPPLETLAAVARHRIAVLMGDQAFNAAFVVPSAVELAAPPARPGQPAALLE
                     RRPDLLVLKAQLDAANWRRQEAKAEYFPRLFLSAVFGRQRLELNDFDLGATRFTNVSG
                     LITMPIFNWGRTQAINEIAEAGQQQSLLRLEDGIVRALEDVENALVALRDERQRATLL
                     QSAAASAEAALGRAQSLYGRGQIDLLPLLDAQRTRLAVQIGANDSSTQLLLDSVQLYK
                     ALGGGWQVFEPGQKEPS"
     gene            1564..>2538
                     /locus_tag="VFX94_07085"
     CDS             1564..>2538
                     /locus_tag="VFX94_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5767983.1"
                     /translation="MKTVLAFTLLALMLTACGPDKPPAEALRTVRTVELRYDKAREAN
                     RYFGSVQARHEVDQAFRVGGKIVQRKVDVGQSVREGDVLAVLDDIDYRHAEEASRQAL
                     IAATAQARQAESDRKRLQALKGDGSVSVSDEEHAESNAEKAKATAEAEARKLELARNK
                     LKYTVLRASRTGVVTVVRAEGGQVVAEGQPVVSIAAQGEPEIVVDVPEDHFAAFKKAQ
                     YKAWLNTAPSESFEVSLRELSLQAAQQTRTYRARLKPVTPRPLPLGATATVVVERTAA
                     DAPMAAIPASAITQAKGQPAVWVVRRDGTELVGTVDLVHVTVHGYRNDE"
BASE COUNT          413 a          862 c          885 g          378 t
ORIGIN      
        1 ccgcgtaggc ggggacccca tttagggttc taagaaccgg ttaattgggt ccccgccttc
       61 gcggggacga cgggtttgcc gttacgaatg taacaaccga aatccgaccg aatgccgacg
      121 cttcggtgac gactgcctgc cgggcttcca ttagcctgat tccatgccgc tacttctgat
      181 tttcgccgtg ctgctcgccg tggccctgac ggggtgctcg acccccttcg tcgagcccga
      241 ggtcgaggtg gccggccgct tcgcgcaggc cgacgcctcg ctggacgagc ccgaagtggc
      301 ctggtgggaa agctacggcg acgcggtgct ctcgagcctc atccggcgtg ccgcccagca
      361 gaaccgcgat atcaagatgg cagccacgcg cgtgcgcgcc gcgcgcgccg gcgagaccat
      421 cagccgctcg aggatgctgc cgaatgtcgg tgtgaccgcc gcgggtaact accagaggac
      481 cgagtacgac gcggccgcga agcaggaggt cccggatgtc aagtcgctaa gcggcggcat
      541 cgatgtttcc tgggagatcg atctttcggg ccgcctgcgc tctgccacct ccgcggctgc
      601 ggctgaagct ttggcggcgg aacacggcga gcggggcgtg cgtcttttgg tgctgaccga
      661 cgtcgccacg aactacttcg tcttggtcgg cgcgtcgcgc cagctcgaga ccgtgcgcgc
      721 gatcgcattg gcgcacgacg aaacgttgcg cctggtcacc gcgcggcagc gcgtcggcct
      781 tgccacgccg ttcgatgtcg agcgcgcgca gaccgaggct tcgcgggcga acgcggcgat
      841 acccccgctc gagacgcttg ccgccgtcgc gcgccaccgc atcgcggtgc tcatgggaga
      901 ccaggcgttc aatgccgcat tcgtcgtgcc ttcggccgtg gagctcgccg ctccgccggc
      961 ccggcccggg cagcccgcag cgctgctcga gcgcaggccc gatctgcttg tgctgaaggc
     1021 gcagctcgac gccgccaact ggcgccggca ggaagccaag gcggaatact tcccgcggct
     1081 cttcctgagc gcagtgttcg gccgtcagag gctcgagctc aacgacttcg acctcggcgc
     1141 cacccgcttc accaatgtat cgggccttat cacgatgccg atcttcaact ggggccgcac
     1201 ccaggccatc aacgagatcg ccgaagccgg ccagcagcag tcgctgctgc gcctggagga
     1261 cggtatcgtg cgcgcgctcg aggatgtcga aaatgcgctc gtggcgctga gggacgagcg
     1321 ccagcgcgcg acgctgctgc agagcgccgc ggcctcggcc gaggcggccc tcggacgtgc
     1381 gcaatcgctc tatggccgcg gccagatcga cctcctgccg ctgctcgacg cgcagcgcac
     1441 gcggctcgcg gttcagatcg gcgcaaacga cagcagcacc cagctactcc tcgacagcgt
     1501 gcagctttac aaggcgctcg gcggcggctg gcaggtgttc gagcccgggc aaaaggaacc
     1561 atcgtgaaaa ctgttctcgc cttcactctc ctcgctttga tgcttaccgc gtgcggccct
     1621 gacaagccgc ccgccgaggc gctgcgcacg gttcgcaccg tggagcttcg ctacgacaag
     1681 gcacgcgagg cgaatcgcta tttcggcagc gtgcaggcgc gccacgaagt ggatcaggct
     1741 tttcgcgtcg gcgggaagat cgtgcagcgc aaggtggacg tcggccagag cgtgcgcgag
     1801 ggcgatgtgc ttgcggtcct cgacgacatc gactaccgcc atgccgagga agcgagccgg
     1861 caggcgctga tcgcggccac cgcgcaggcg cgccaggcgg agtcggaccg caaacgcctc
     1921 caggcgctga agggcgacgg ctcggtcagc gtgtcggacg aagagcacgc cgagagcaac
     1981 gcggagaagg cgaaagccac ggccgaggcc gaagcgcgga agctcgagct cgcgcgcaac
     2041 aagctgaagt acacggtgct gcgcgcatcg cgcaccggcg tcgtgaccgt cgtgcgggcc
     2101 gagggcgggc aggtcgtcgc cgagggccag ccggtcgtct cgatcgccgc ccagggcgag
     2161 cccgagatcg tggtcgacgt gccggaggat cacttcgccg ccttcaagaa ggcgcagtac
     2221 aaggcctggc tcaataccgc gcccagcgag agcttcgagg tcagcctgcg cgagctctcg
     2281 ctgcaggcgg cgcagcagac tcgcacctac cgcgcgcggc tgaagccggt gacgccgcgc
     2341 ccgctgccgc tcggcgcgac cgccacggtg gtggtcgagc gcacggcggc ggacgcgccg
     2401 atggccgcga tcccggcgag cgcgatcacg caggcgaagg gccagccggc ggtgtgggtc
     2461 gtgcgccgcg acggaaccga gctggtggga acggtcgatc tcgtgcatgt cacggttcat
     2521 ggctaccgca acgacgag
//
LOCUS       DASUNT010000352         8347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_49476, whole genome shotgun sequence.
ACCESSION   DASUNT010000352 DASUNT010000000
VERSION     DASUNT010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8347
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49476"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VFX94_07090"
     CDS             complement(<1..925)
                     /locus_tag="VFX94_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5767984.1"
                     /translation="MNWLLSGDFPRSRALSILLLLIFAGLLFAPFVFPGTRSLNVAAK
                     VAVFILLVASYDLLLGYTGVVSFAHTMFFGIGGYGVGIAMAKMSSTWGSLLAGIALSL
                     LLTLLIAAAIGILSLRVRAIFFAMITLAVASFFQILASQLSDFTGGEDGINFRLPELL
                     RHRLTAYYTVFAVALVLFLVLLRVVNSPFGRVLLSIRENEFRSEALGYRTVAYRTVAN
                     CLAALVAAVAGALYALWLRYVGPDTTLSFSIMLDILLMVVIGGMGTMYGAVIGATLFI
                     VAQNYLQKLMGAATKATEALPLLPSLLHPDRW"
     gene            complement(922..1869)
                     /locus_tag="VFX94_07095"
     CDS             complement(922..1869)
                     /locus_tag="VFX94_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5767985.1"
                     /translation="MVPVIALAALPLIASVPTWVTLTVAGLAMGMMIFIMASGLTLVF
                     GLMDVMNFAHGAFISVGAYAAATILVAMKTGMQAASLSANLAAIGPAIVAAIALAGAL
                     GWFFERVVIRPVYGDHLKQILVTMGGLIVVEQLIHVVWGPDIIPLPRPATLTGAVLFG
                     DAAIERYRMLAVAVGLAVFVAMWWLLGRTKLGLLIRAGVENTEMVESLGYRIRRLFVG
                     VFVAGSALAGLGGVMWAMYQESVNATMGMQVMVVIFIVIIIGGLGSVGGCFVGALLVG
                     LVANYIGYVAPKLALGSNILLMVLILLWRPRGLYPLGKA"
     gene            complement(1893..2612)
                     /locus_tag="VFX94_07100"
     CDS             complement(1893..2612)
                     /locus_tag="VFX94_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5767986.1"
                     /translation="MADLLQLAGVNTHIGQYHILQGVDFAVARNSLTMLLGRNGAGKT
                     TCLRTIMGLWSASAGTIRYQGREIQTLKTPDVARLGIAYVPENMGIFAGLTVRENMLL
                     AAREHRPDEKRLDWIFGLFPALKKFWHTHAGNLSGGQKQMLAIARAMVEPRKLLLVDE
                     PTKGLAPAIIGAMIEAFRELKRDATLLVVEQNFHFARSLGDTVAVMDSGRIVHTGAMA
                     ALAADPALQQRLLGLSLDSHQ"
     gene            complement(2605..3360)
                     /locus_tag="VFX94_07105"
     CDS             complement(2605..3360)
                     /locus_tag="VFX94_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5767987.1"
                     /translation="MEPVLETRDLTIRFGGHVAVDAVSCAFRPGTLTAIVGPNGAGKT
                     TYFNLISGQLPATSGSVRLFGEDVTRLPAATRTRRGIGRAFQLTNLFPRLSVLENVRL
                     AVQARAGMGRNLISPWASHVELIRHAEQYLERVALAEKRAASASALPHGDQRKLEVAI
                     LLALEPEVFMFDEPTAGMSVEEVPVVLDLIRAIKQSADKTVLLVEHKMEVVRSLADRI
                     VVLHQGRLVADGDPATVIASPIVQEAYLGVGRG"
     gene            complement(3357..4535)
                     /locus_tag="VFX94_07110"
     CDS             complement(3357..4535)
                     /locus_tag="VFX94_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5767988.1"
                     /translation="MRQLRFALLALAAGAGAAYGQAREIKIAHIYGKTGALEAYAKQT
                     HAGLMMGLEYATQGKMELLGRKIVVIEKDDQLKPDVGKAHLAAAYGDDKVDLAVGPTS
                     SGVALAMLPVAEEYKKVLLVEPAVADAITGERWNRYVFRTGRSSSQDAISNAVALGKP
                     NTFIATLAQDYAFGRDGVRAFKEALAGTGAQIVHEEYAPQQTTDFTAPAERLFNALKE
                     RKGRKVIFIIWAGGNPFKIADLNPGRVGIEGASGGNILPALAAYKALPGMEGATYYYY
                     EHPKNPVNKWLVDEHQKRFKSPPDFFTAGGMAAGIAIVEGIKRAGSTDTEKLIAAMEG
                     MAFETPKGRMTFRKEDHQALQSMYHFKIKVDPNVQWAIPELVREIKPDEMKVPIRVKQ
                     "
     gene            complement(4562..5734)
                     /locus_tag="VFX94_07115"
     CDS             complement(4562..5734)
                     /locus_tag="VFX94_07115"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEX5767989.1"
                     /translation="MYRLWLTFAQAATIAIAVLFVVSAFHPEWLPSRAVVVQQAPVPP
                     APTTGPLPAAVRAGSFHEAVQRAAPSVVNISTAKELRSSRHPLLNDPLFRRFFGSQPP
                     EDAAAQRAFSLGSGVVVSAGGYVLTNHHVVDGADEIEVALHDGKKLLAKVVGSDPETD
                     LAVLRVSAENLPAISFGSSEALRVGDVVLAIGNPFNLGHTVTSGIVSALGRSGLNAAT
                     FENFIQTDAAINPGNSGGALIDAGGNLVGINTLIISPTGGAAGIGFAIPVSTAKMVLE
                     QIIRTGSVTRGWIGVELRDITPLDAASLQLGAQRGALIAVVLRGGPADKAGVRPGDVA
                     VEVQGKPVGDATAMLNVIAALVPGEPARMKLRRHGQDVEATVNVGRRPRPQPRAAD"
     gene            5861..6460
                     /gene="petA"
                     /locus_tag="VFX94_07120"
     CDS             5861..6460
                     /gene="petA"
                     /locus_tag="VFX94_07120"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015420298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX5767990.1"
                     /translation="MNEEPKADKTRRNLVVATTLVGGAASVAAAIPFVASMWPSERAK
                     AAGAPVEADISGIGPGEMAVIEWRGKPVWVLRRTKEMIASLKTLEPRLTDPASKASEQ
                     PKYAENEYRSAKPELMVMEGVCTHLGCSPQLKSAEAKAEMGADWLGGFYCPCHGSKFD
                     FAGRVFRGAPAPINLKVPPYTFVSDSTLLIGEDTETKGA"
     gene            6509..7765
                     /locus_tag="VFX94_07125"
     CDS             6509..7765
                     /locus_tag="VFX94_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX5767991.1"
                     /translation="MPSVAEQWKIHAADYYAPKNFNFWYFFGSLAMLVLVIQIVSGIF
                     LTMNYKPDANLAFGSVEYIMRDVPGGWLIRYIHSTGASAFFIVVYLHMFRAMLYGSYR
                     QPRELVWIFGVLLYLTLMGEAFFGYLLPWGQMSYWGAQVIVNLFGTVPFIGPDLALWI
                     RGDYVVSDATLNRFFALHVIALPLVLLGLVVAHILALHDVGSNNPDGVEIKKLKGPDG
                     KPLDGIPFHPYYTVKDLVGAVVFLAIFCVVTFFVPEMGGYFLEHNNFIPADPLKTPEH
                     IAPVWYFTPYYSILRAVPPLFNSQFPGVLAMGVAVVILFFLPWLDRSPVKSIRYRGPL
                     YKTWLAAFVIVFVLLGYLGIEPTTVWGQFPVRDDSFLGRYIVDTKDIATWVARVCTVV
                     YFLFFFLMPWYTARDKVKPVPERVTV"
     gene            7762..>8347
                     /locus_tag="VFX94_07130"
     CDS             7762..>8347
                     /locus_tag="VFX94_07130"
                     /inference="COORDINATES: protein motif:HMM:NF014247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEX5767992.1"
                     /translation="MKALLLLLLVLPGVAAAATGYRLDRSPHDPRDVVSLQAGARTYI
                     NYCLGCHSLQYMRYQGLTDLGISEALVKEYLLFTGDKIGDPIRTSGFEAKTGKSWFGV
                     PPPDLSVIARSRGADWLYTYLRTFYRDPKTVTGWNNAVFPNVAMPHALWKLQGERGWD
                     AKDGRFTEISQGALSVVEYDRTVSDLVNFLVYVSE"
BASE COUNT         1469 a         2726 c         2735 g         1417 t
ORIGIN      
        1 gccagcggtc cgggtgcagc aggctcggca gcaggggcag cgcctcggtc gccttggtcg
       61 cggcccccat cagtttctgc agatagttct gggcaacgat gaacaaggtg gcgccgatca
      121 ccgcgccgta catcgtgccc atgccgccga tgacgaccat cagcaggatg tcgagcatga
      181 tcgaaaagga aagggtggtg tcggggccga catagcgcag ccacagcgca tagagcgcac
      241 cggccactgc cgcgaccagc gccgccaggc agttggccac ggtgcgatag gcgacggtgc
      301 ggtagccgag cgcttcagac ctgaactcgt tctcccggat ggagagcagc acgcggccga
      361 atggggagtt cactacgcgc aaaagcacaa ggaagaggac cagggcgacc gcgaacaccg
      421 tatagtaggc cgtaaggcgg tggcgcagca gctcgggtag ccgaaagttg atgccgtcct
      481 cgccaccggt gaagtcggaa agctgcgaag caagaatctg gaaaaaagac gctacggcga
      541 gcgtgatcat cgcgaagaaa atggcgcgca cgcgcagcga caagatgccg atcgcagccg
      601 caatgagcag ggtgagcaga agcgacagcg cgatccccgc aagcaggctg ccccaggtgg
      661 aactcatctt tgccatggca atgcccaccc cgtagccgcc gatgccgaaa aacatggtat
      721 gcgcgaagga cacgacgccg gtatagccaa gaagcaggtc atagctcgcg acgagcagga
      781 tgaacaccgc caccttggcc gctacgttaa gcgagcgtgt gcccggaaag acgaaaggcg
      841 cgaaaagcag gccggcgaaa atcagcagga gcaggatcga cagcgcgcgg ctgcggggga
      901 agtcgcccga cagcagccag ttcatgcctt gccgagcggg tagagtccgc gcggccgcca
      961 cagcagtatg agcaccatga gcaggatgtt cgagccgagc gcgagcttcg gcgcgacgta
     1021 gccgatgtag ttcgccacca gtccgacgag cagtgcgccg acgaaacagc ctccgaccga
     1081 gcccagcccg ccgatgatga tgacgatgaa gatcaccacc atcacctgca tccccatggt
     1141 ggcgttgacg gactcctggt acatcgccca catcacgccg ccgagcccgg cgagcgccga
     1201 gccggcaacg aacacgccaa cgaacagccg gcggatgcgg tagccgagcg attccaccat
     1261 ttcggtgttt tccacgccgg cgcggatcag cagtccgagc ttcgtgcggc cgaggagcca
     1321 ccacatcgcc acgaataccg cgaggccgac cgctaccgcg agcatgcgat agcgctcgat
     1381 ggcagcgtcg ccgaaaagca ccgcgccggt cagcgtggcc ggacgcggca gcggaatgat
     1441 gtccggcccc cagaccacgt gaatcagctg ctcgacgacg attaggccgc ccatggtcac
     1501 gaggatctgc ttgagatgat cgccgtacac cggccggatc accacgcgct cgaagaacca
     1561 gccgagcgcg ccagcgagcg caatcgccgc gacgatcgcc ggaccgatgg ccgccaggtt
     1621 tgcactcagg ctggcggcct gcatgccggt cttcatcgcc accaggatcg tggccgcggc
     1681 atacgcgccg accgagatga acgcaccgtg cgcgaagttc atgacgtcca tcaggccgaa
     1741 caccagcgtg agcccggacg ccatgatgaa gatcatcatg cccatggcaa ggcctgcgac
     1801 cgtgagggtg acccaggtcg ggacgctcgc aatgagcggc agggcggcga gcgcgatcac
     1861 tgggaccatc agcacgggaa tccagtcggt cttcactggt gcgagtcgag cgacaggcca
     1921 agcaggcgct gttgcagcgc cgggtcggcg gcgagcgccg ccatcgctcc ggtatgcaca
     1981 atgcgaccgg agtccatcac ggccaccgtg tcgccgaggg agcgcgcgaa atggaaattc
     2041 tgctccacca cgagaagcgt ggcgtcgcgc ttgagctcgc ggaacgcctc gatcatcgcg
     2101 ccgatgattg cgggcgcgag ccccttggtc ggctcgtcga cgaggagcag cttgcgcggc
     2161 tcgaccatgg cgcgcgcgat tgcgagcatc tgcttctggc cgccggagag gttgccggcg
     2221 tgcgtgtgcc agaacttctt cagcgccgga aagaggccga agatccaatc gaggcgcttc
     2281 tcgtcgggcc ggtgctcacg tgcggcaagc agcatgttct cgcgcaccgt cagcccggca
     2341 aagatgccca tgttttccgg cacgtaagcg atgccgaggc gggcgacatc gggcgtcttc
     2401 aacgtctgga tctcgcgccc ttggtagcgg atggtcccgg cggaggcgct ccacaggccc
     2461 atgatcgtgc gtaggcaggt cgttttgccg gcgccgttgc gtcccaggag catggtgagc
     2521 gagttgcgcg caacggcgaa gtccacgccc tgcaggatgt ggtattgccc gatatgcgtg
     2581 ttcacgccgg caagctgcag cagatcagcc acggccgacg ccgaggtagg cttcctgcac
     2641 gatcggtgag gcgatgaccg tggccggatc gccgtcggca accaggcgtc cctggtgcag
     2701 taccacgatg cggtcggcga gcgatcgcac tacttccatc ttgtgctcga ccagcagcac
     2761 ggtcttgtcg gcgctttgct tgatggcgcg gatcaggtcg agcacgaccg ggacctcctc
     2821 gacgctcatt ccggccgtcg gttcgtcgaa catgaagact tcgggctcga gggcaagaag
     2881 gatcgcgacc tcgagcttgc gctggtcgcc gtgcggcaag gccgacgcgc tcgccgcgcg
     2941 cttctcggcg agcgccacgc gctcgaggta ttgctcggca tgacgtatca gctcgacgtg
     3001 gctagcccag ggcgatatca ggttgcggcc cattccggcg cgcgcctgca cggcgagccg
     3061 gacattttcg agaacgctga ggcgcggaaa caggttcgtc agctggaaag cgcgcccgat
     3121 gccgcgccgc gtgcgcgtcg ctgcaggcag ccgggtgacg tcctcgccga agaggcgcac
     3181 gctgccgctc gtcgcgggca gctgtccgga gatgagattg aagtaggtgg tcttaccggc
     3241 gccgttcggc ccgacgatcg ccgtgagcgt gccggggcga aaagcgcagc tcacggcgtc
     3301 taccgcgaca tggcccccga agcggatggt caggtcgcgg gtttcgagga caggctctat
     3361 tgcttgaccc tgatcgggac cttcatttcg tcgggcttga tctcgcgcac gagctccggg
     3421 atcgcccatt gcacattggg gtcgaccttg atcttgaagt ggtacatcga ctgcagcgcc
     3481 tggtggtctt ccttgcggaa ggtcatgcgg cccttcggcg tctcgaacgc catgccttcc
     3541 atcgcggcaa tcagcttttc ggtatcggtc gagcccgcac gcttgatacc ctcgacgatg
     3601 gcgatgccgg ccgccatgcc gcccgcggta aagaagtcgg gcggcgactt gaagcgcttc
     3661 tggtgctcgt cgaccagcca cttgttgacc gggttcttgg ggtgctcgta gtagtagtac
     3721 gtcgcgcctt ccatgcccgg gagcgccttg taggccgcga gcgcgggaag aatgttgccg
     3781 cccgaggcac cctcgatacc gacgcgcccc gggttcaggt cggcgatctt gaacggattg
     3841 ccgccggccc agatgatgaa gatcaccttg cgccccttgc gctccttcag cgcgttgaag
     3901 aggcgctccg ccggagccgt gaaatcggta gtctgctggg gcgcgtattc ctcatgcacg
     3961 atttgcgcgc cggtgcctgc gagcgcctcc ttgaacgcgc gtacgccgtc gcggccgaat
     4021 gcataatcct gtgcgagcgt ggcgatgaag gtgttcggct tgccgagcgc caccgcattg
     4081 gagatcgcgt cctgcgacga tgagcgaccg gtgcggaata catagcggtt ccagcgctct
     4141 ccggtaatcg cgtcggcgac ggcgggctcg acgagcagga ccttcttgta ctcctccgcg
     4201 acaggcagca tcgcgagcgc cacccccgag ctcgtcggcc cgacggcaag gtcgaccttg
     4261 tcgtcgccgt acgcggcggc gaggtgcgcc ttgcccacat cgggcttgag ctggtcgtct
     4321 ttctcgatca ccacgatctt gcgcccgagg agctccatct tgccctgggt ggcgtactcc
     4381 aggcccatca tcagcccggc atgcgtctgc ttcgcgtacg cctccagcgc cccggtcttg
     4441 ccatagatgt gggcgatctt gatctcgcgc gcctggccgt acgcggcgcc ggcgccggcc
     4501 gcgagcgcga gcaatgcaaa gcgaagctgt ctcatcgttt tccccggttg gtgcagcgat
     4561 tttagtctgc ggcgcgcggt tgcgggcgcg gccggcggcc gacgttcact gtcgcttcga
     4621 cgtcctgccc gtggcgcctg agcttcatcc tggccggctc gcccggcacc agcgcagcga
     4681 tcacgttaag catcgcggtc gcatccccga cgggttttcc ctgcacctcg accgccacat
     4741 cgccgggccg cacgcccgcc ttgtcggccg ggccgccgcg caaaacaaca gcgatcagcg
     4801 cgccgcgctg cgcgccgagc tgcagcgatg ccgcgtcaag gggcgtgata tcgcgcagct
     4861 cgacgccgat ccagccgcgc gtgaccgatc cggtgcggat gatctgctcg agcaccatct
     4921 tcgcggtgga caccggaatc gcgaagccga tgccggcggc gccgccggtc ggcgagatga
     4981 tcagcgtgtt gatcccgacc aggttgccgc cggcgtcgat gagcgcgcca ccggaattgc
     5041 ccgggttgat cgccgcatcc gtctggatga aattctcgaa cgtcgccgca ttgagccccg
     5101 agcgcccgag cgcggagacg atgccgctcg tgaccgtgtg gccgaggttg aacgggttgc
     5161 cgatggcgag gaccacgtcg cccacgcgca gcgcttcgga ggagccgaag ctgatcgccg
     5221 gcaggttctc ggcgctcacg cgaagcaccg cgaggtcggt ctcggggtcg ctgccgacca
     5281 ccttggcgag cagcttcttc ccgtcatgca gcgcgacctc gatctcgtcg gcgccatcga
     5341 ccacgtgatg gttcgtgagc acgtagccgc cggcgctcac gactacccct gaaccgaggc
     5401 tgaaggcgcg ctgggcggcg gcgtcttcgg gcggctggct gccgaagaac ctgcggaaca
     5461 gcggatcgtt aagcagcgga tggcgcgagg agcgaagctc cttcgcggtg gagatgttga
     5521 cgaccgacgg tgcggcgcgc tgcaccgctt cgtggaagga ccccgcgcgc accgcggcgg
     5581 gcagcggccc ggtcgttgga gcgggtggca ccggcgcctg ctgcacgacg acggcgcgcg
     5641 aaggtagcca ctccgggtgg aacgccgata ccacgaagag caccgcaata gcgatcgttg
     5701 cggcctgcgc aaaggtgagc cagaggcgat acatgacgga acttaagtaa agcagatttt
     5761 ccctgagaat ccaatcgttt aggagcgaaa acgtggactt ttgtaggggc gctcgctaga
     5821 atcgcaggct tttgcgccct tccatcagca cgccatcccc atgaatgaag aaccaaaagc
     5881 ggacaagacg cggcgcaacc tggtcgtcgc cacgacgctc gtcggcggcg ccgccagcgt
     5941 ggcggccgca attcccttcg tagcgagcat gtggccctcg gagcgcgcca aagcggccgg
     6001 ggctccggtc gaggcggaca tttccggcat cggaccgggg gagatggcgg tgatcgaatg
     6061 gcgcggcaag cccgtctggg tcctccgccg caccaaagag atgatcgcgt cgctgaaaac
     6121 gctggagccg cggctcaccg atccggcctc caaggcttcg gagcagccga agtacgccga
     6181 gaacgaatac cgctcggcaa agcccgagct catggtgatg gaaggcgtgt gcacgcacct
     6241 cggctgctcg ccgcagctaa agagcgccga ggcgaaggcc gagatgggcg ccgactggct
     6301 gggcgggttc tactgcccgt gccacggctc gaaattcgac ttcgcgggcc gcgtgttccg
     6361 cggcgcgccg gcgcccatca acctgaaggt gccgccgtac acgttcgtca gcgacagcac
     6421 tctgctcatc ggcgaagaca ccgaaaccaa gggggcatag gccatggcat cgcgtctgca
     6481 ggcgctgtgg ggctggttcg acgcgcgcat gccatcggtg gcggagcagt ggaagatcca
     6541 cgccgccgac tactacgcgc caaagaactt caacttctgg tacttcttcg gttcgctcgc
     6601 gatgctggtg cttgtgatcc agatcgtctc gggcatcttc ctcaccatga actacaagcc
     6661 cgacgccaac ctcgcgttcg gctccgtcga gtacatcatg cgcgacgtgc cgggcggatg
     6721 gctcatccgc tacatccact ccaccggcgc ctccgcgttc ttcatcgtcg tgtacctgca
     6781 catgttccgc gccatgctgt acggctcgta ccggcagccg cgcgagctgg tgtggatctt
     6841 cggcgtattg ctctacctca cgctcatggg agaggctttc ttcggctacc tgctgccgtg
     6901 gggccagatg tcgtactggg gcgcgcaagt catcgtgaac cttttcggca ccgtgccctt
     6961 catcggcccg gacctcgcgc tctggatccg cggcgactac gtcgtgtcgg acgcgacgct
     7021 caaccgcttc ttcgcgctgc acgtgatcgc gctgcctctc gtgctgctcg gcctcgtggt
     7081 cgcgcacatc ctcgcgctgc acgacgtcgg ctcgaacaac ccggatggcg tggagatcaa
     7141 gaagctgaag ggcccggacg gcaaaccgct cgacggcatt ccgttccacc cgtattacac
     7201 ggtgaaggac ctggtcggcg cagtggtgtt cctcgccatc ttctgcgtcg tcacgttctt
     7261 cgtgcccgag atggggggct acttcctcga gcacaacaac ttcattccgg cggacccgct
     7321 caagacgccg gaacacatcg cgccggtgtg gtacttcacg ccgtactact cgatcctgcg
     7381 cgccgtgccg ccgcttttca actcgcagtt tcccggcgtc ctcgccatgg gagtcgcggt
     7441 ggtgatcctt tttttcctgc cgtggctaga tcgcagcccg gtgaagtcca ttcgctaccg
     7501 cggccccctt tacaagacct ggctcgccgc tttcgtgatc gtgttcgttc tgctcggcta
     7561 cctcggcatc gagccgacga ccgtctgggg gcaattcccg gtgcgcgacg actccttcct
     7621 cggccggtac atcgtcgaca ccaaggacat cgcgacctgg gtggcgcgcg tctgcaccgt
     7681 cgtgtacttc ctctttttct ttctgatgcc ctggtacacg gcgcgcgaca aggtaaagcc
     7741 cgtgccggaa cgggtaacgg tatgaaagct ttgctgcttt tactgctggt tctccccggg
     7801 gttgcggcag cggccaccgg ctatcggctg gatcgttcgc cgcacgaccc gcgcgacgta
     7861 gtgagcctgc aggcgggcgc gcgcacctac atcaactact gcctcggctg ccacagcctc
     7921 cagtacatgc gctatcaggg gctcaccgat ctgggcatca gcgaagcgct ggtgaaggaa
     7981 tacctgctct ttaccggcga caagatcggc gacccgatcc gcacgagcgg cttcgaggcg
     8041 aagacaggca agtcctggtt cggcgtgccg cccccggacc tttcggtcat cgcgcgctcg
     8101 cgcggcgcgg actggctgta cacgtacctt cgcaccttct accgcgaccc gaagaccgtc
     8161 accgggtgga acaacgccgt attccccaat gtcgccatgc cgcacgcgct gtggaagctg
     8221 cagggcgagc ggggctggga cgccaaggat ggccggttca ccgagatctc gcagggcgcg
     8281 ctgtcggtgg tggagtacga ccgcacggtg agcgacctgg tgaacttcct cgtctacgtc
     8341 agcgagc
//
LOCUS       DASUNT010000353         9139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_49553, whole genome shotgun sequence.
ACCESSION   DASUNT010000353 DASUNT010000000
VERSION     DASUNT010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9139
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49553"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFX94_07135"
     CDS             complement(<1..282)
                     /locus_tag="VFX94_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767993.1"
                     /translation="MRGFINVEQLEEYMTRPSAELEADLAGIGDILVLGVGGKMGPTL
                     ARMAKRAAPGSRVIGVARFSEKRATPITRLSGAARFAMRASVGPILPPTP"
     gene            306..1316
                     /locus_tag="VFX94_07140"
     CDS             306..1316
                     /locus_tag="VFX94_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX5767994.1"
                     /translation="MRSPRIQTTVVGSYPIPQWLARAPSEQALVDATRVVLHTQEQAG
                     IDLVCDGELYRFDLNPPETNGMIEYFVRPMSGVRGDIGFSEWLDYSRGSGMRFRTRPP
                     GVVDGPIGPGTLNLKIPCARAKALATRPLKFTVTGPHMLAKTLHDRHYKSPEKLAHAI
                     AEVLAAQMKHLDADVIQLDEANLPGHPDEWEWAAAAVNKVLKSVPKKSKAAVHLCFGN
                     YGGQTIQKGTWSKLIDYLNALKVDHIVMETAHRPPAELAVFKSLRKDIGFGLGVVDIK
                     RTEIEKPDDIARAIERAEKMLGKGRVKYIHPDCGFWMLPRNVADGKIRALPAGRDLYL
                     GS"
     gene            complement(1380..2714)
                     /locus_tag="VFX94_07145"
     CDS             complement(1380..2714)
                     /locus_tag="VFX94_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5767995.1"
                     /translation="MLHDARGYAVTATNPKALEAYERALYAFRSYRGDPIAMINEAIA
                     LDAGFAAAYAAKALMLITVFERRFMRDALATLQQGRDALQLATAREQALAGAARRIAL
                     GEWREGLRALEQVLIEYPRDLVALQTAHILDFMRGDALNLRNRVSRVLPAWSPALPGY
                     AFVLGMHAFGYEECNQYPEAERAGRKAVELGGDDSWAVHGVAHVMEMQGRIGDGLAWY
                     AQTRSVWAGEDNGFAFHNAWHTALFHMDRGELARALEIYDQRFGGPLELALVRIDATA
                     LLWRLKLEGVDVASRFAAVVESWNTVLEDERGFYAFNDYHMALACAAAGRRELLAKLR
                     EGLERAQLDNAEMTRVVGRPVLEAAIAYGEGRYGAAVDLIAAARDTAMVSGGSHAQRD
                     LLTLTLIDAATRAGMKTLARHYAHERLVHKPESAWGRRLVQRIEARDAKLAA"
     gene            complement(3440..4759)
                     /locus_tag="VFX94_07150"
     CDS             complement(3440..4759)
                     /locus_tag="VFX94_07150"
                     /inference="COORDINATES: protein motif:HMM:NF042664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HEX5767996.1"
                     /translation="MANGKGTCFVVMGFGKKTDFESGRTLDLDKSYRNVIKPAVVAAG
                     YDCVRADEIVHSGLIDVPMYEQLLAADIVVADLSTSNKNAFYELGVRHALRPYTTIVI
                     AEDGMKPPFDVNHVVVRSYKHLGEDIGYDEVMRFRGLLTGAIATIAALAEDKRADSPV
                     YRFVSGLKPPGIGAAVSATQAQPAPTSTHSALMKQVNDAIDKNDFLSAKSLLGAIRTL
                     IRNEAPGRPEDPYIVQRLAFVTYKSRHPSEVAALKEASMLLASLEPDTSNDTETLGLW
                     GAVHKRLWALTQDITHLDQAVRALERGFYLRNDYYNGINLAFLLNVRASRATDPADAI
                     TDFMLARRVRQEVLTICEKWLTDNPAMGASTPEPARKQQAETRYWVLATLGEAMVGLD
                     DPAAPKRLDAIYRSAPAPWMADSTREQIGKLQTLLADPPLKHLKALG"
     gene            complement(4801..5382)
                     /locus_tag="VFX94_07155"
     CDS             complement(4801..5382)
                     /locus_tag="VFX94_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5767997.1"
                     /translation="MTEPRRVFLFSGHLIDARGRKAPRFPPEREPIAAGAIAAALDEL
                     GAGSADLGITEGACGGDLLFAEALLERGAALELRLPFDERTFLRESVDFEKPPSSMPG
                     RWHERFVQVKARAKALVMPEVLGPTPDGQSPYERCNRWMLDDALAMGVEKLTFICLWD
                     GGGGDGPGGTRHMVEAVRSQGGEVRWLDTRKLW"
     gene            5441..5644
                     /locus_tag="VFX94_07160"
                     /pseudo
     CDS             5441..5644
                     /locus_tag="VFX94_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354244.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
     gene            complement(5641..6045)
                     /locus_tag="VFX94_07165"
     CDS             complement(5641..6045)
                     /locus_tag="VFX94_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5767998.1"
                     /translation="MNLASRFVDVEELPWEKTRYPGVEQKTLLVEKETGLLTALMRMA
                     PGAKLPDHEHVKIEQTYVLQGELHCGEGICKAGQFVWRPAGSRHHAWAGPQGGLFLAI
                     FQAPNKFFQADGAVTDFLGRDWEKTWGSKPQF"
     gene            complement(6042..7481)
                     /gene="pyk"
                     /locus_tag="VFX94_07170"
     CDS             complement(6042..7481)
                     /gene="pyk"
                     /locus_tag="VFX94_07170"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004188973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX5767999.1"
                     /translation="MVRSTKIVATLGPASSEPVTLERMIRAGVDVVRLNFSHGTADDH
                     LKRAALVKQISAKVGRTVAIMCDLQGPKIRIGKFRDGKTTLEKGQPFILDAGAEVGDG
                     QRVGLDYKELPRDVESGALLLLDDGKIVLEVADVRGDEVHTVVRHGGILSNNKGINRQ
                     GGGLTAPALTAKDMDDIKIAVKLQADFLAVSFPKSGADMYMARELMRAAGGKAFIIAK
                     IERAEAVAEAALIDIMRGSDGIMVARGDLAVEVGDASVPALQKKMIRLAREQNKLTIT
                     ATQMMESMISSPVPTRAEVSDVANAVLDGTDAVMLSAETASGRYPVEVIEAMDRICVE
                     AEQTQPLNLEQDFLNRVFTRVDQSIAMAALFTAYHLRVKCIAALTESGSTALWMSRLN
                     CGVPIYALTSQTGTRYRCALFRDVFPLMVKYVGHDRDELLDEAEKALVENMVVKEGDL
                     IVLTIGEPIGKAGGTNTMKIVKVGEHRMP"
     gene            complement(7502..8296)
                     /locus_tag="VFX94_07175"
     CDS             complement(7502..8296)
                     /locus_tag="VFX94_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX5768000.1"
                     /translation="MKIDADIVGREARAEMVHLDKRWIMAYNAALGEMSAEPHPLFPV
                     CYEWDATRALRESTGLQALNANLVHAQHELVIHRAPRVDERVSIVARVVSANERKPGA
                     FVTIRLAARGVDGALVSTSHYGMLYRGVALEGNSRAIEGLEDPPKHASPLRKIGDVPI
                     AATAAHVYTECARIWNPIHTDPEYARAAGLPGIILHGTATLALSLTALQKDFGKTTVT
                     RVRCRFAGMVLMPTTLSVYAAAEDTWIAFETRNGSNEAVIERGWIS"
     gene            complement(8293..8700)
                     /locus_tag="VFX94_07180"
     CDS             complement(8293..8700)
                     /locus_tag="VFX94_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX5768001.1"
                     /translation="MVEQLIVETSGQGLLEITPKVNAVVAHAGIDEGVCTIFVRHTSA
                     SLVIQENADPSAKRDLERWLNRLVPEGDPFYEHDTEGPDDMPSHIKAALTATSLSVPI
                     MKKRLALGPWQGIYLWEHRRRGSHRELVVHIGN"
     gene            complement(8693..>9139)
                     /gene="pgk"
                     /locus_tag="VFX94_07185"
     CDS             complement(8693..>9139)
                     /gene="pgk"
                     /locus_tag="VFX94_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX5768002.1"
                     /translation="LAEPDLAGEAKRIMKRTKVPLPSDVVVEGGKAKPVEAVGKDEMI
                     LDIGPDTAKAFAAELKNAGTIVWNGPVGVFEKDAYAKGTQGLARAIAGSKAFSIAGGG
                     DTVAAIHKFKVDRRIDYISTAGGAFLEFLEGKKLPAVAALEARADG"
BASE COUNT         1505 a         3033 c         2965 g         1636 t
ORIGIN      
        1 cggcgtcggc ggcaagatgg gcccgacgct cgcgcgcatg gcgaaacgcg ccgcgccgga
       61 gagccgcgtg atcggcgtcg cccgcttttc ggagaagcgc gccactccga tcacacggct
      121 ccccggcgcg gcgcgcttcg ccatgcgcgc gagcgtcggg cccatcttgc caccgacgcc
      181 gagaaccagg atatcgccga tgccggcgag atcggcctcg agctcggccg agggccgcgt
      241 catgtactct tcgagctgct caacattgat gaagccgcgc atgagcgaat tctatgatcc
      301 gtgaaatgag atcgccgcgc atccagacga cggtggtcgg ctcgtatcct attccgcagt
      361 ggctcgcgcg cgcgccgtcg gagcaggcac tggtcgatgc gacgcgcgtc gtgctgcaca
      421 cgcaggagca ggccgggatc gatctcgtgt gcgacggcga gctctaccgc ttcgacctca
      481 acccccccga gaccaacggc atgatcgagt acttcgtgcg cccgatgtcg ggcgtgcgcg
      541 gcgacatcgg cttctcggag tggctcgact actcgcgcgg ctcgggcatg cgctttcgca
      601 cccggccgcc gggcgtcgtc gacggcccca tcggacctgg cacgctcaac ctcaagatcc
      661 cgtgcgcccg cgccaaggcg ctcgctacgc gcccgctcaa gttcacggtg accgggccgc
      721 acatgctcgc gaagacgctg cacgaccggc actacaagag ccccgaaaag cttgcgcacg
      781 cgatcgcgga ggtcctcgcc gcgcagatga agcacctgga tgccgatgtg attcagctcg
      841 acgaagccaa ccttcccgga catcccgacg agtgggaatg ggcagcggca gccgtcaaca
      901 aagtcctgaa atccgttccg aaaaaaagca aagccgcggt gcacctctgc ttcggcaact
      961 acggcggcca gacgatccag aaaggaacct ggtcgaaact gatcgactac ctgaacgcgc
     1021 tcaaggtcga ccacatcgtc atggagacgg cgcaccgccc gcccgcggag ctcgcggtgt
     1081 tcaagagcct gcgcaaggac atcggcttcg gcttgggcgt ggtggacatc aagcgcaccg
     1141 agatcgagaa gcccgacgac atcgcgcgcg ctatcgagcg agcagagaaa atgctcggca
     1201 agggccgcgt gaagtacatc caccccgatt gcggcttctg gatgctgccg cgcaacgtcg
     1261 ccgacggaaa gatccgcgcc cttcccgccg gacgggatct gtacctgggg tcctgaccga
     1321 aaatcggggt cagcgccccc ggcaatcgcc gggatagcga gttctttggc gagaatcttc
     1381 taggccgcga gcttcgcgtc gcgcgcttcg atccgctgca cgaggcgcct gccccatgcg
     1441 ctctcgggct tgtgcacgag gcgctcgtgg gcgtagtggc gggcgagcgt tttcatgccg
     1501 gcgcgcgtgg ccgcatcgat aagcgtgagc gtgagcaggt cgcgctgcgc gtggctgccg
     1561 cccgagacca tcgcggtgtc gcgcgcggcg gcgatcaggt ccacggcggc gccgtagcgg
     1621 ccctcgccgt aggcgattgc cgcctcgagc accggccggc cgacgacgcg cgtcatctcg
     1681 gcgttgtcga gctgggcgcg ctcgaggccc tcgcgcagct tggcgagcag ctcgcgccgg
     1741 cccgcggccg cgcaggcgag cgccatgtgg tagtcgttga aggcgtagaa gccgcgctcg
     1801 tcctcgagca ccgtgttcca gctctcgacg accgcggcaa agcgggaagc cacgtccacg
     1861 ccctcgagct tcaggcgcca cagcagcgcg gtcgcgtcga tgcgcacgag ggcgagctcg
     1921 agcggcccgc cgaaacgctg gtcgtagatt tcgagcgctc ttgcgagctc gccgcgatcc
     1981 atgtgaaaaa gcgccgtgtg ccaggcgttg tggaaggcga agccgttgtc ctcgcccgcc
     2041 cacacgctgc gcgtctgcgc gtaccaggcg aggccgtcgc cgatgcgccc ctgcatctcc
     2101 atcacatggg ccacgccgtg caccgcccag ctgtcgtcgc cgcccagctc cactgccttg
     2161 cggccggcgc gctcggcttc cggatattgg ttgcattcct cgtagccgaa ggcgtgcatg
     2221 ccgaggacga acgcgtagcc gggaagcgcg ggactccagg cgggaagcac gcgcgagacg
     2281 cggtttctga gattcagcgc gtcgccgcgc atgaagtcca gaatgtgagc ggtctgcagc
     2341 gccaccaggt cgcgcgggta ctcgatcagc acctgctcca gggcgcgcag tccctcgcgc
     2401 cattcaccca gcgcgatgcg ccgcgcggcg ccggcgagcg cctgctcgcg cgccgtggcg
     2461 agctgcaggg cgtcgcggcc ctgctgcagc gtggcgagcg cgtcgcgcat gaagcggcgc
     2521 tcgaacacgg tgatcagcat cagcgccttc gccgcgtagg cggcggcgaa gcccgcatcc
     2581 agggcgatcg cctcgttgat catggcgatg gggtcgccgc ggtaggagcg gaaggcgtac
     2641 agcgcgcgct cgtaggcttc gagcgccttg ggattggtgg cggtgacggc gtagccgcgt
     2701 gcgtcgtgaa gcatttcggt ctccttaaaa acgaggcgca tgctaacaag caccaacggc
     2761 caccggggtt gtcgtttggt tgtgaggttg taaccggcgc gtttcagccg cgcgctgatc
     2821 accgatcctc gggtccgacc gggcgcggac ctgtacctgg gtcgccgacc cggaaaatcg
     2881 ggtcagagca catgaacgac tgtaacgagc agtacgctat cgcttggagg tcagcatccg
     2941 ctcggcgagc gtgctcgcct cgtcggcgcc ggcggcatcg gctttcatct cattccccgc
     3001 aggcgttgat gctcgtatag ccgaaatcca tcgcgacgcc tgctcgaagc ctcggtagtg
     3061 gtaagcgagc gaagtctcgc gccagcccca gcgtttcttg atcggtcgtg gtcttgagat
     3121 cgtcggcaga ctgcaatacg cgctccgcat catccagctt ctgctcggcc ggaagatcgg
     3181 cgtccttgta ggtgcaaagc gcgagcttct gcgcgacctt cagtcacagc ttcgtatcgc
     3241 gcaagatgtc cgggtttatc ccgaatgcgc tccagtatct tcgggcaagc ccgaacctgc
     3301 gggcgccgcg gaggactttc tccaagccga gcacttcttt gggtgatgtc gcagcgccag
     3361 cgataattcc ccgcgcttgg cgctcggcat cttgcagggc catcgtcatg ggtcctccca
     3421 aagccgttgt acgaccgatc taaccgagcg ctttcaaatg cttgagcggc gggtcggcga
     3481 gcaacgtctg cagctttccg atctgttcgc gggtactgtc cgccatccac ggcgcgggcg
     3541 ccgagcgata gatggcgtcg agccgctttg gcgcggcagg gtcgtcgagg ccgaccatcg
     3601 cttcgccgag cgtggcaagc acccagtacc gcgtttcggc ttgctgtttg cgcgccggtt
     3661 cgggtgttga ggctcccatt gccgggttat cggtcagcca cttctcgcaa atcgtaagaa
     3721 cctcttggcg gacccgccgc gcaagcatga agtcagtgat tgcatcagcc gggtcggtcg
     3781 cacgcgacgc acgcacgttg aggagaaagg cgagattgat accgttgtaa tagtcgttgc
     3841 ggagatagaa gccgcgctcc agggcgcgta cggcttggtc caggtgggtg atgtcctgcg
     3901 tgagggccca caggcgcttg tggacagctc cccacaggcc gagcgtctcg gtatcgttcg
     3961 aagtgtccgg ttccagcgaa gcgagtagca tcgacgcctc tttcaatgct gcgacctcgc
     4021 ttggatgcct gctcttgtag gtgacgaacg cgaggcgctg aacgatgtag ggatcttctg
     4081 gacgaccggg cgcctcgttc ctgatcagcg tgcggatggc gccaagcagg ctcttcgcgc
     4141 tgaggaagtc gttcttgtcg atcgcatcgt tcacctgctt catgagcgcg ctatgcgtcg
     4201 aagtcggcgc cggttgcgcc tgcgtcgccg ataccgcagc gccaatcccg ggcggcttca
     4261 gtccgctcac gaatcgatac accgggctat cggcccgctt gtcctcggcc aacgcggcga
     4321 tggtcgcgat cgcgccggtg agaaggccac ggaaccgcat gacttcgtcg tagccgatgt
     4381 cctcgcccaa atgcttgtag gaacgtacaa cgacgtggtt cacgtcgaat ggcggcttca
     4441 tgccgtcctc ggcgatgacg atcgtcgtgt atgggcgcaa tgcgtgacgg acgccgagct
     4501 cgtagaacgc gttcttgttc gacgtggaaa gatccgcgac cacgatgtcg gccgccagga
     4561 gctgctcgta catcgggacg tcgatcaggc ccgagtggac gatctcgtcg gcgcgcacgc
     4621 aatcgtagcc ggccgcgacg accgcgggct tgatgacgtt gcgatacgac ttgtcgagat
     4681 ctaaggtgcg gccagattcg aaatcggtct tcttgccgaa ccccattact acgaagcagg
     4741 tgcccttgcc atttgccatc gtccccctcc tcctgcagat cctcgcgagc tatgcggccc
     4801 tcaccatagc ttcctggtgt cgagccaacg aacctcgcca ccttgcgagc gcaccgcctc
     4861 taccatatgg cgcgttccgc cgggaccgtc gccgccgccg ccgtcccaga gacagatgaa
     4921 ggtcagcttc tcgacaccca tcgcgagcgc gtcatcgagc atccaacgat tgcagcgttc
     4981 atacgggctt tgaccatcgg gagtcggtcc cagcacctcg ggcattacga gagctttcgc
     5041 gcgtgctttc acctgcacga agcgctcatg ccagcgcccg ggcatggacg acggcggctt
     5101 ctcgaaatcg accgactcgc gcaagaacgt tcgctcgtcg aacggcagcc ggagctcgag
     5161 cgcagcaccg cgctcaagca gtgcttcggc aaagagcaga tccccgccgc aagccccctc
     5221 cgtgatgcca aggtcggcac tacccgcccc aagctcatca agcgcggcgg cgatggcacc
     5281 cgcggcgatc ggctcgcgct ccggcgggaa gcgtggcgcc ttccgtcccc tcgcgtcgat
     5341 gagatgccca ctaaagagaa atacgcggcg cggttcggtc atcactggtg ctctgttact
     5401 agatctggca tctgggccgc gcacgcaaac cgcaagaaac cgactactcg agccgggtga
     5461 tatcgaggaa gggctgaacg cagcggcagc ggtcggcgac gaccggttgc aagaggccgc
     5521 gcgcggccgc gtctcgcccg agtcgttcac ccacggcagt gcccggcagc gcgcggactg
     5581 gttccgtcgc gggctcgaga gcggcgagct gagggcgtgc gatacattcg ccgcacggct
     5641 ctagaactgg ggctttgagc cccaggtttt ctcccagtcg cggccgagga agtcggttac
     5701 cgcgccgtcg gcctggaaga acttgttcgg cgcctggaag atcgcgagga aaagccctcc
     5761 ctgcggcccg gcccaggcat ggtggcggct gcccgcgggg cgccagacga actgacctgc
     5821 cttgcagatc ccctcgccgc aatgcagctc gccttgcagt acatacgttt gctcgatctt
     5881 cacgtgctcg tgatcgggca gcttggcgcc cggcgccatg cgcatgagcg cggtgagcag
     5941 gccggtctcc ttttccacca gcagcgtctt ctgctccacg ccgggatagc gcgtcttctc
     6001 ccagggcagt tcttccacat cgacaaatct ggaagcgagg ttcatggcat ccggtgctcc
     6061 ccgactttca cgatcttcat ggtgttcgtg ccgcccgcct tgccgatcgg ctcgccgatg
     6121 gtgagcacga tcaggtcgcc ctcctttacc accatgttct cgaccagcgc cttctcggcc
     6181 tcgtccagga gctcgtcgcg atcgtggccg acgtacttga ccatcagggg gaacacgtcg
     6241 cggaagagcg cgcagcggta gcgcgtcccg gtctgcgagg tgagggcata gatcggcacg
     6301 ccgcagttca ggcggctcat ccacagcgcg gtggagcccg actcggtgag cgcggcgatg
     6361 catttcaccc tgaggtggta cgcggtgaag agcgccgcca tcgcgatcga ctggtcgacg
     6421 cgggtgaaca cgcggttgag gaagtcctgc tcgaggttga gcggctgcgt ctgctccgcc
     6481 tcgacgcaga tgcggtccat ggcctcgata acctccaccg ggtagcgccc gctcgcggtc
     6541 tcggccgaaa gcattaccgc gtcggtgccg tcgagcacgg cgttcgccac gtcggaaacc
     6601 tcggcgcgcg tgggcacggg gctcgagatc atcgactcca tcatctgcgt cgccgtgatg
     6661 gtgagcttgt tctgctcgcg cgcgaggcga atcattttct tctgcagcgc cggcaccgac
     6721 gcgtccccga cctccacggc aagatcgccg cgcgccacca tgatgccgtc ggagccgcgc
     6781 atgatgtcga tgagcgccgc ctcggccacg gcttcggcgc gctcgatctt ggcgatgata
     6841 aacgccttgc cgccggcggc gcgcatgagc tcgcgcgcca tgtacatgtc ggcgccgctc
     6901 ttcgggaacg agaccgcaag aaagtccgcc tgcagcttca ccgcgatctt gatgtcgtcc
     6961 atgtccttcg cggtgagtgc cggagcggtc agcccgccgc cctggcggtt gatgcccttg
     7021 ttgttggaca gtatcccgcc gtggcgcacg acggtatgca cctcgtcgcc gcgcacgtcg
     7081 gcaacttcga gaacgatctt gccgtcgtcg agaaggagga gcgcgccgga ctcgacatcg
     7141 cgcggcagct ccttgtaatc gaggccgacg cgctggccgt cgcccacttc ggcaccggca
     7201 tcgaggatga acggctggcc tttctcgagc gtggtcttgc catcgcggaa tttgccgatg
     7261 cggatcttgg gcccctggag gtcacacatg attgccacgg tgcggccgac cttggccgag
     7321 atttgcttca ccagagcggc gcgtttcaga tggtcgtccg ccgttccgtg cgagaagttg
     7381 aggcgcacga cgtccacgcc ggcgcggatc atgcgctcga gcgtgaccgg ctcgctcgaa
     7441 gcgggaccga gggtggcgac gatttttgta ctgcgcacca tgaaggaaag tccgccggag
     7501 tttaactgat ccagccgcgc tcgatgaccg cctcgttgct cccgttgcgc gtctcgaacg
     7561 caatccacgt gtcttccgcg gctgcgtaca ccgagagcgt agtcggcatg aggaccatgc
     7621 cggcaaagcg gcagcgcact cgcgttacgg tggtctttcc gaaatccttc tgcagtgcgg
     7681 taagtgaaag cgccaaggtg gccgtaccgt gcaggatgat ccccggaaga cctgccgcgc
     7741 gggcgtactc cggatcggtg tggatggggt tccatatgcg cgcgcactcg gtgtagacat
     7801 gcgccgccgt ggccgcgatc ggaacatcgc ctatcttccg aagcggactt gcgtgcttgg
     7861 gaggatcttc cagtccctcg atcgcccggc tgttaccttc gagcgcgacg ccgcggtaca
     7921 gcatgccata gtggctcgtg ctcaccagcg cgccgtccac gccgcgggca gcgagccgaa
     7981 tggtgacgaa ggcacccggc ttgcgctcgt tcgccgagac cactcgcgcg acgatgctca
     8041 ccctctcgtc caccctggga gcccgatgga tgacgagttc gtgctgcgcg tgcacgaggt
     8101 tcgcgttcaa agcctgaagc ccggtcgact cgcgcagcgc gcgcgtggcg tcccattcgt
     8161 aacacaccgg gaagagcgga tgcggctcgg cgctcatttc gccgagcgcg gcgttgtaag
     8221 ccatgatcca gcgcttgtcg agatgcacca tctcggcgcg cgcctcgcgc ccgacgatgt
     8281 ccgcgtcgat cttcaattgc caatgtgcac tacgagttcg cgatgcgagc cgcggcggcg
     8341 gtgctcccac aggtagatgc cttgccaggg gccgagcgcc aggcgtttct tcatgatggg
     8401 aaccgacagc gaagtcgcgg tgagcgcggc cttgatgtgc gaaggcatgt cgtccgggcc
     8461 ctcggtgtcg tgctcgtaga aaggatcgcc ctcgggcacc aggcgattga gccagcgctc
     8521 cagatcccgc ttcgccgaag gatcggcatt ctcctggatc accaggctgg cggaagtgtg
     8581 ccgcacgaag atcgtgcaca cgccttcgtc gattccggca tgagccacca ccgcgttcac
     8641 cttcggggtt atttcgagca ggccctggcc ggaggtctcg acgatgagct gctcaaccat
     8701 ccgcgcgcgc ttcgagagcg gcgaccgcgg gaagcttctt gccctccagg aactcgagga
     8761 acgcgccgcc cgcggtcgag atgtagtcga tgcgcctgtc caccttgaac ttgtggatcg
     8821 cggccaccgt gtcgccgccg ccggcgatgg agaacgcctt ggacccggca atcgccctgg
     8881 cgagcccctg cgtgcccttg gcgtaggcgt ccttctcgaa cacgcccact ggcccgttcc
     8941 agacgatggt gccggcgttc ttgagctccg cggcgaatgc cttggccgtg tccggtccga
     9001 tgtcgaggat catctcgtcc ttccccaccg cctcgaccgg ttttgccttt ccgccctcca
     9061 ccaccacgtc gctcggcagc gggaccttgg tccttttcat gatgcgcttc gcctcgccgg
     9121 cgaggtcggg ctcggcgag
//
LOCUS       DASUNT010000354         2484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_488614, whole genome shotgun sequence.
ACCESSION   DASUNT010000354 DASUNT010000000
VERSION     DASUNT010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488614"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..540)
                     /gene="dut"
                     /locus_tag="VFX94_07190"
     CDS             complement(91..540)
                     /gene="dut"
                     /locus_tag="VFX94_07190"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX5768003.1"
                     /translation="MRKLEVRILDERMRGMLPHYASRGAAGLDLRACLESPLVLQSGD
                     SQLVSSGIAIHVGDPGYAAIILPRSGLGAKSGIVLGNLVGLIDSDYQGPLMISVWNRG
                     RQPFTIQPLDRIAQLVVVPVVQVEFEVVEEFAASARGAGGFGSTGKS"
     gene            complement(542..1747)
                     /gene="coaBC"
                     /locus_tag="VFX94_07195"
     CDS             complement(542..1747)
                     /gene="coaBC"
                     /locus_tag="VFX94_07195"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX5768004.1"
                     /translation="MTLTGKRILLGITGGIAAYKSAELTRLLVKDGADVRIAMTEAAT
                     HFIGTATLQALSGQPVWTDLWDPRVKDAMGHIELSRDRELIVVAPASADFMAKLAHGL
                     ADDLLSTLCVARRCALMVAPAMNVEMWQNAATARNAETLRGDGIQIVGPASGGQACGE
                     LGMGRMTEPADILADIRFFFQAQELEGKLRGKRVLVTAGPTEEPIDPVRVLTNTSSGK
                     MGYAIARAAREAGAEVTLISGPVSLATPAGVARVDVRTAREMFDAVKKDVKSADIFIS
                     VAAVADYRVKSPSAQKIKKGNGHLLLELEENPDILAWVAALPKPPFCVGFAAESENLA
                     QNARAKLAKKKLPLIVGNLAQDALGGDDSAMTLYDAAGEHPLGRGPKLELARRLLDHL
                     AGMLPPKTR"
     gene            1804..2475
                     /gene="radC"
                     /locus_tag="VFX94_07200"
     CDS             1804..2475
                     /gene="radC"
                     /locus_tag="VFX94_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX5768005.1"
                     /translation="MAISDWRPEDRPRERLLAQGAGALSNAELLAVLLRTGVRGKSAV
                     DLAHDILRQCEGVTRTLEAGAELEAIKGLGPAKRAQFAAAVELARRALQEHLAENAAL
                     NSPGAVRDYLRLKLAARKEEAFVCIWLDAQHRAIDFEVAFQGTLTHTSVYPREIVRRA
                     LERNAAAVIFAHNHPSGVAQPSRSDELLTRDLKDALALVEVKVLDHFVVAGNQALSFA
                     ERGLL"
BASE COUNT          438 a          843 c          780 g          423 t
ORIGIN      
        1 ttcattgaag gctcccttta gtgatctgac gtctcgcaac atcagcatcg cctaacagcc
       61 ttcaatgaac aacctatcga gaaatcacac ctagctcttc cccgtgctgc cgaagccgcc
      121 ggcgccgcgt gcgctggcgg cgaattcctc caccacctcg aactcaacct gcaccaccgg
      181 cacgaccact agctgcgcga tgcggtcgag cggctggatg gtgaacggct gcctgccccg
      241 gttccacacc gaaatcatga gcggcccctg gtaatcggaa tcgatcaggc ccaccaggtt
      301 gccgagcacg atgccgcttt ttgcgccgag acccgagcgg ggcaggatga tcgccgcata
      361 gccggggtcg ccgacatgga tcgcaatccc ggaggatacg agctggctgt cgccggactg
      421 cagaaccaag ggcgattcca ggcacgctcg caagtcgagg cccgccgccc cccggctcgc
      481 gtagtggggc agcatgccgc gcatcctctc gtcgaggatc ctgacctcca gctttctcat
      541 ttcaccgcgt tttcggcggc agcatgccgg cgagatggtc caacagcctg cgcgccagct
      601 ccagcttcgg cccccggccg agcggatgct cgcccgcagc gtcgtagagc gtcatggcgc
      661 tgtcgtcgcc accgagggcg tcctgcgcca ggttgccgac gatcagcggc agtttcttct
      721 tcgcgagctt cgctcttgcg ttctgcgcaa ggttctcgct ctccgcggcg aaacccacgc
      781 agaatggcgg ctttggcaaa gcggcgaccc aggcgaggat gtccgggttc tcctcgagct
      841 cgagcaagag gtgaccgttt cccttcttga tcttctgcgc tgacgggctc ttgacccggt
      901 agtcggcgac tgcagcgacc gagatgaaga tgtcggcgct cttcacgtcc ttcttcacgg
      961 cgtcgaacat ctcgcgcgcg gtacgcacgt cgacgcgtgc cacgccggcc ggggtggcca
     1021 gcgaaaccgg gccgctgatc agtgtgacct ccgcacccgc ctcacgcgcc gcacgcgcga
     1081 tcgcgtaacc catcttgccg gagctcgtat tcgtgaggac gcgaaccggg tcaatcggct
     1141 cctcggtcgg acccgccgtg acgagcacgc gcttgccccg cagcttgccc tccagctcct
     1201 gcgcctgaaa aaaaaaccgt atgtcggcca ggatatctgc cggctcggtc atgcggccca
     1261 tgcccagttc tccgcaagcc tggccacccg acgcgggtcc cacgatctgt atcccgtcgc
     1321 cgcgcagcgt ttcggcattg cgcgcggtcg ccgcgttctg ccacatctcc acgttcatcg
     1381 ccggcgccac catcagcgca cagcgccgcg ccacgcaaag cgtcgacagc agatcatcgg
     1441 caaggccgtg cgcgagcttt gccatgaaat cggccgatgc gggcgcaacg acgatcagct
     1501 cccgatcgcg tgagagctcg atatgcccca tggcgtcctt gacgcgcgga tcccagaggt
     1561 cggtccacac gggctgaccg gacagcgcct gcagagtcgc ggtaccgatg aagtgcgtcg
     1621 ccgcctcggt catggcgatg cgcacgtccg cgccgtcctt caccagcagg cgcgtcagct
     1681 cggcggattt gtacgccgcg atgccgccgg taatgccaag cagaatccgc ttgcccgtca
     1741 gtgtcatccg atgtgccacc tgccccgtta agggggaaac gattctaagg gaaaggggag
     1801 aacatggcga tttcggactg gcggccagag gaccggccgc gtgaacggct gcttgcccaa
     1861 ggagcaggcg cactgagcaa tgcggagctg ctggccgtcc tcctgcgcac cggcgtgcgc
     1921 ggcaagagcg cggtcgatct ggcgcacgac atcctcaggc aatgcgaggg cgtgacgcgc
     1981 acgctcgaag ccggtgccga gctcgaagcg atcaaggggc tcggtcctgc gaagcgcgcg
     2041 caattcgccg ccgcggtgga gctcgcgcgg cgggcgctgc aggagcatct ggcggaaaat
     2101 gcggccctca actcgcccgg ggccgtgcgc gactacctgc gcttgaagct cgctgcgcga
     2161 aaggaagagg ctttcgtatg catctggctc gacgcgcagc atcgggcaat cgacttcgaa
     2221 gtcgccttcc aaggaacact gacgcacacc agcgtctacc cccgcgagat agtgaggcgc
     2281 gcgctagaaa gaaacgccgc ggcggtgatc tttgcgcaca atcatccatc gggggttgcg
     2341 cagccgagtc ggtcggacga gctgctgacg cgcgacctga aggacgcgct ggcgctggtg
     2401 gaggtgaagg tgctcgacca tttcgtggtc gccggcaacc aggccctctc cttcgcagag
     2461 cgcggattgt tatagtaaaa tcgc
//
LOCUS       DASUNT010000355         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1278537, whole genome shotgun sequence.
ACCESSION   DASUNT010000355 DASUNT010000000
VERSION     DASUNT010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278537"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /gene="cgtA"
                     /locus_tag="VFX94_07205"
     CDS             <1..690
                     /gene="cgtA"
                     /locus_tag="VFX94_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HEX5768006.1"
                     /translation="ADGALVADLVQDGARALLARGGQGGLGNVHFKSSVNRAPRQFTR
                     GEPGESRSLSLELRVLADVGLLGLPNAGKSSFIRAVSSARPKVADYPFTTLNPSLGVA
                     RAGETSFVLADIPGLIEGASEGAGLGHQFLRHLARTRLLLHLVDVTERDPARDARVIG
                     AELKKYGQGLERKPRWLVFNKIDAVEDPDAVIRRALSRLRWKRPWFKVCALSGEGCAA
                     VVKAVARELSK"
     gene            687..1796
                     /gene="proB"
                     /locus_tag="VFX94_07210"
     CDS             687..1796
                     /gene="proB"
                     /locus_tag="VFX94_07210"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX5768007.1"
                     /translation="MIRDAKMLVVKVGSSLVTNEGRGLDAGAIGRWAAQVAQLNRLGK
                     KTVLVSSGAIAEGMQRLGWSRRPHAMHELQAAAAVGQMGLAQCYESCLRRHGLHSAQV
                     LLTNADMADRQRYLNARSTLRTLLQLNVIPVINENDTVATDEIKLGDNDTLAALVTNL
                     IEADALIILTDQAGLFDADPRRNQHARVVDEADATDPRLESLAGGAGSALGKGGMLTK
                     VLAARRAARSGAHTVIACGAEPDVLVRLAGGERIGTLLTAQTVPLAARKQWLADRLAL
                     SGRVRLDAGAVKALTRDGKSLLPVGVTEVGGEFRRGDVVACLDPAGREIARGLVNYSA
                     AEARQIMRKPSGEIEAILGYVDEPELIHRDNLVLL"
     gene            complement(1793..2293)
                     /locus_tag="VFX94_07215"
     CDS             complement(1793..2293)
                     /locus_tag="VFX94_07215"
                     /inference="COORDINATES: protein motif:HMM:NF020331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEX5768008.1"
                     /translation="MKRRAAAFCLAVASACGQTEEKSDWEREHEKRLPPSENEVSLPA
                     YPRERDLIEFSAGPTSEFRYFVDANSVSVGSDGIVRYTLVARSPAGVENVSYEGMRCT
                     SAELRVYALGRERSWRVTQSAWRPMEPRSMQAWHRVLYRDYFCRYREPVASREQALYV
                     LRGDRR"
BASE COUNT          384 a          818 c          830 g          356 t
ORIGIN      
        1 gctgacggcg cgctggtcgc cgacctggtg caagacggcg cgcgcgcgct gcttgcgcgc
       61 ggcggccagg gcgggctcgg aaatgtgcac ttcaaatcca gcgtgaaccg cgcgccgcgc
      121 cagttcacgc gcggcgagcc cggcgaaagc cgcagcctct cgctcgagct gcgagtgctc
      181 gccgacgtag gcctgctcgg cctgccgaac gccggcaagt cgagctttat ccgcgccgtc
      241 tccagcgcac gcccgaaggt ggctgactac ccgttcacga cgctgaaccc ctcgctcggc
      301 gtggcgcgcg caggcgagac gagcttcgtc ctcgccgata tcccggggct catcgagggc
      361 gcatccgaag gcgcgggtct cggccaccag ttcctgcggc atctcgcgcg cacgcggctc
      421 ctgctgcacc tcgtcgatgt caccgagcga gatccggcgc gcgacgcgcg ggtgatcggc
      481 gccgagctga agaagtacgg ccaggggctc gagcgcaagc cgcgttggct ggtgttcaac
      541 aagatcgacg cggtcgagga tccggatgcc gtcatccggc gcgcgctgtc ccggttgcga
      601 tggaagcgcc cctggttcaa agtttgcgcc ctcagcggcg aaggttgcgc cgcggtcgta
      661 aaggcggtcg cgcgggagct ttcgaaatga ttcgggacgc gaagatgctg gtagtaaagg
      721 tcggctcgtc gctcgtcacg aacgaggggc gcgggctcga tgcgggcgcc atcggccgct
      781 gggcggcgca ggttgcgcag ctcaaccggc tgggaaagaa gaccgtgctg gtatcgagcg
      841 gggcaatcgc cgaaggcatg cagcggctgg ggtggtcgcg gcgcccgcac gccatgcacg
      901 agctgcaggc tgcggccgcg gtcggccaga tgggtcttgc gcagtgctac gagagctgct
      961 tgcgccgcca cgggctgcac agcgcacagg tgctgctcac caacgccgac atggccgacc
     1021 ggcagagata tctcaatgcg cgctcgacgc ttcgcacgct cctccagctc aacgtgatcc
     1081 cggtcatcaa cgagaacgac accgtggcga ccgacgagat caagctcggc gacaacgaca
     1141 cgctggcggc cctcgttacc aacctgatcg aagcagacgc gctcatcatc ctgaccgatc
     1201 aggcggggtt gttcgacgcc gatccgcgca gaaatcagca tgcgagggtc gtcgacgaag
     1261 ccgacgccac cgatccgcga ctcgagagcc tggccggcgg tgcgggcagc gcgcttggca
     1321 agggcgggat gctcaccaaa gtgcttgcag cgcgacgcgc agcgcgcagc ggcgcccaca
     1381 ccgtgatcgc ctgcggcgcg gagcccgatg tcctcgttcg gcttgccggt ggcgagcgca
     1441 tcggcactct gctcaccgcg cagaccgtgc cgctcgcagc acgcaagcag tggctcgccg
     1501 accggctggc cctcagcggc cgcgtgcggc tcgatgccgg ggccgtaaag gcgctgacgc
     1561 gcgacggcaa aagcctgctg ccggtcggcg tcaccgaggt tggcggcgaa ttccggcgcg
     1621 gcgatgtggt cgcctgcctg gacccggccg ggcgcgagat cgcgcgcggg cttgtgaact
     1681 actcggcggc cgaagcgcga cagataatgc gcaagccatc aggcgagatc gaggcgatcc
     1741 tcggctacgt ggacgagccc gagctcatcc atcgagataa tctcgtcctg ctctagcgac
     1801 gatcaccgcg caggacgtag agcgcctgtt cgcggctggc gaccggctcg cgataccggc
     1861 agaagtagtc gcgataaagc accctgtgcc aggcctgcat gctgcgcggc tccatcggcc
     1921 gccatgcgct ttgcgtaacc ctccagctgc gctcccggcc gagcgcgtag acgcgcagct
     1981 ccgcgctcgt gcaacgcatg ccctcgtagc tcacgttctc caccccggca gggctgcgcg
     2041 cgaccagcgt gtagcgcacg atgccgtcgg atccgacgct gacggaattg gcgtcgacga
     2101 agtagcgaaa ctcgctcgtc ggcccggcgg agaactcgat caggtcgcgc tccctggggt
     2161 aggcgggaag cgaaacctcg ttctccgagg gcggcaggcg cttttcgtgc tcgcgttccc
     2221 agtcgctttt ttcctcggtc tgaccgcagg cgctcgccac ggcgaggcaa aacgcggcgg
     2281 cgcgccgctt caagcgagat cgctggcgag cgcggcgcgc acctcgggcg cgacctcgac
     2341 ggctgcgcgg tactgcgggt gttccacgcc gatcctcacc gccgcccc
//
LOCUS       DASUNT010000356         5202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_50267, whole genome shotgun sequence.
ACCESSION   DASUNT010000356 DASUNT010000000
VERSION     DASUNT010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50267"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VFX94_07220"
     CDS             complement(<1..458)
                     /locus_tag="VFX94_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5768009.1"
                     /translation="MSDLSSLRIQSMRERVSPDEWRTRIDLAACYRLVHHYGMDDLVY
                     NHISARVPGEEGHFLINAYGLMYDEITASSLVKVDFEGNVVLDSGTGYGINQAGFVIH
                     SAVHKGRPDVGCVIHTHSIAGMAVSALDCGLLPITQTAMYFGGVGIHEYE"
     gene            650..1255
                     /locus_tag="VFX94_07225"
     CDS             650..1255
                     /locus_tag="VFX94_07225"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX5768010.1"
                     /translation="MNALVQGWRKVVFAGFLVAAAYTARAQDLDRPVLLVAQPDLQGM
                     YSRTALLAVPTPGGQHVGFIINRATDVKLATLFPDHAPSGKVVNPVYFGGPEMLGAIF
                     AIVQRDPGVPSLRLFGDVFVTGNSDAVDRIIEQTPNDARYFAGFVGWQTGELAKELEA
                     GYWYVTDPDPALVFSDEAGGAMWEALLKRAGKPALPGTNSI"
     gene            1352..1912
                     /locus_tag="VFX94_07230"
     CDS             1352..1912
                     /locus_tag="VFX94_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5768011.1"
                     /translation="MAADFRAEVEAMRAYLLRYASLQLRNPDAAEDVVQETLLAALAA
                     ERSFEGRANLRTWVTGILKHKIIDTIRRESRGRPLDESDEERGASDFDGLFNQRGHWH
                     EHSEPWQDPDGALHQTQFLRALEACLAGLPARTSRVFLMREHMGMETAEICKELGLTS
                     THCWVLLYRARMALRECLEKTWFKAR"
     gene            1909..2091
                     /locus_tag="VFX94_07235"
     CDS             1909..2091
                     /locus_tag="VFX94_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006766719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5768012.1"
                     /translation="MNISCKEASRLMSQAQDRELSLGERASLKFHLVLCRGCRAFKGQ
                     LELLRRAMRELSAGRN"
     gene            complement(2301..2465)
                     /locus_tag="VFX94_07240"
     CDS             complement(2301..2465)
                     /locus_tag="VFX94_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768013.1"
                     /translation="MSRYIARWTKGRTPAQLAASAARATVIAVVPGVLWIWLAWCLVR
                     QLNKARLRAR"
     gene            2596..5040
                     /locus_tag="VFX94_07245"
     CDS             2596..5040
                     /locus_tag="VFX94_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5768014.1"
                     /translation="MASEAAVSAGPAALSARRGEAFYRALVEGMNDGVLTRDAAGVIT
                     YASPRFRDMLGYSAEELVGGKSQFVLPPEQRSRWERGLAAAAQGPQRFEHDLVCKDGR
                     RVAVAVSRRPVFDAEGVFQGSVALVSNITEERRASRLLQEVAEATAPHVGEEFFRAAV
                     RFLAQALRCKIVFITECVDFPTTRVRTVARWALDRLADNVEYDLADTACEETIKAGKV
                     FYCASGVESRFAAARGLGVDSYLGLPLFGTEDHVIGHLAFVHDAPIDRQLLENPVVRI
                     FASRAEAELRRKRADDTMLLIGRAVAPLSGEVFFRTLIEQLVRTFGFRQAFISECLDY
                     PPTRVRTVAYWDGNGLRENTEFDLAGLPCEITLGDARPYFVPERLEELYPAEAGRRRV
                     SYLGLPIFHADRKQVVGHLAFFDERPRPGNVLDSPAFRIIASRAGVELLRKRAEDELR
                     ESEAKYRLLVQNQTDLLVKLDRERRYIFVSPSFCKCFGKNERELLGRPFDLEVNPLDR
                     GAFEQAHAAVLAPPYRSRCEARVLSAKGWQWLAWECSAILDERGEVGEIIASGRDVTE
                     RRRAEEQARQHLQQLAHVTRVASMGEMASAIAHEINQPLAAITTYTQACVRLLRSGSA
                     SLDEIIETMERVGARAERASEVIRHLRSFVRKEDTQLIPVQINFLVGEIVRLVQPEAA
                     QSGVEITTDLGEGLPAVMADNIQIEQVLLNLVRNAIDAICAARMERREVRIVTRLSAD
                     GAVAASVHDSGPGFDGETAGRLFEPFFTTKVHGMGIGLSISQSIVEAHHGRIWATGEP
                     GRGASFYVALPAASAG"
BASE COUNT          815 a         1741 c         1804 g          842 t
ORIGIN      
        1 ctctcgtact cgtgaatgcc gacgccgccg aagtacatcg cggtctgcgt gatcggcaga
       61 agcccgcagt cgagtgcgga caccgccatg ccggcgatcg aatgggtatg gatcacgcag
      121 ccgacgtccg ggcgtccctt gtgcacggcg ctgtggatga cgaagccggc ctggttgatg
      181 ccgtagccgg tgccggagtc cagcaccacg ttgccttcga agtcgacctt caccaggctc
      241 gacgcggtga tctcgtcgta catcaggcca taggcgttga tgaggaaatg cccttcttcc
      301 cccggtacgc gggccgagat gtggttgtag acgaggtcgt ccatcccgta gtgatggacg
      361 agccggtaac aggcggcaag atcgatacgc gtgcgccatt cgtcgggcga gacgcgctcg
      421 cgcatcgact ggatcctgag ggaggagagg tcgctcatgt gcctattatg cggcgcgtca
      481 ctgccgccac gtgaattcgc gcaacattgc tgcaccgctc atctcgtcga gcgccccgtc
      541 cgtcagagcg ccttgcaata gcccgcgatg agcgcagttt ttcatagccc tctctacacg
      601 ccgcgcgcat ggtggaccca cgggcagcag gcccgccatg gaggtgcaca tgaacgcgct
      661 tgtgcaaggc tggagaaagg tcgtgttcgc aggctttctg gtcgccgccg cttacacggc
      721 gcgcgctcag gatctcgatc gtccggtgct cctcgtggcc cagccggacc tgcaggggat
      781 gtatagccgg accgcgctcc tcgcggttcc gacgccgggg gggcagcacg tcggcttcat
      841 catcaaccgc gccacggacg tgaagctcgc gacgcttttt cccgaccacg cgccctcggg
      901 caaggtggta aatcccgtgt acttcggcgg ccccgaaatg ctgggcgcca tcttcgccat
      961 cgtgcagcgc gatccgggtg tgccgtcgct gcgtcttttc ggcgacgttt tcgtcaccgg
     1021 caacagcgat gcggtcgacc gcatcatcga gcaaacgccg aacgacgcgc gctacttcgc
     1081 cggcttcgtc ggctggcaga cgggcgagct cgccaaggag ctggaggccg gctactggta
     1141 cgtgaccgac cccgacccgg cgctcgtttt cagcgacgag gcgggcggcg cgatgtggga
     1201 agcgctgctg aagcgcgcgg gcaagcccgc gctgccgggc acgaactcga tctaaaaaag
     1261 gcgctcgaga tccaggtgcc tccggtgctc ctcgcgagca ccgaccgcat caccgattag
     1321 tagcccacct gccggcctat acttggcgcg cgtggctgcc gacttccggg ccgaggtaga
     1381 agcaatgcgc gcctacctgc tgcgctacgc ctccttgcag cttcgcaatc cggacgcggc
     1441 ggaggacgtg gtgcaggaga cgctgctcgc cgcgctcgcg gcggagcgca gcttcgaagg
     1501 gcgcgcgaac ctgcgcacct gggtcaccgg gatcctgaag cacaagatca tcgacacgat
     1561 ccggcgggag agccgcgggc ggccgctgga cgagagcgac gaggagcgcg gcgcctccga
     1621 cttcgacggg ctcttcaacc agcgcgggca ctggcacgag cattccgagc cgtggcagga
     1681 ccccgatggc gcgctccacc agacgcagtt cctgcgggcg ctggaagcct gcctcgcggg
     1741 gctgccggcg cgcacctcgc gggtgttcct catgcgggag cacatgggca tggagacggc
     1801 tgaaatctgt aaggaattgg gtctgacgtc gacccattgc tgggtccttc tttaccgggc
     1861 gcgcatggcg ctgcgcgagt gcctggagaa gacctggttc aaagccagat gaacatctct
     1921 tgcaaggagg cgtcccgcct catgtcgcag gcgcaggatc gcgagctgtc gctgggcgag
     1981 cgtgcctcgc tcaagttcca cctcgtcctc tgccgcggct gccgtgcgtt caaagggcag
     2041 ctcgagctcc tgcgtcgggc gatgcgcgag ctctcggccg ggcgcaattg acgatcaagt
     2101 tggcgcgcgg ctatccggtc cgggcctgag ctacagcgtc cattcaggtt gcgcttagcc
     2161 cgcggccgcc gttcagcagc ggctcgacgg tgcggcttgc gcgacgctga catgggcatg
     2221 cgccaacccg ccgagcaatg cgaagcctgc agacagcgct ttgataagct tggtcatggt
     2281 cggtctccgt agcagcgggt tcaccgggcg cgcagccgag ccttgttcag ctggcgcacc
     2341 aggcaccagg cgagccaaat ccacagcacg cccggcacca ccgcgatcac ggtggcgcgc
     2401 gcggccgacg cggcgagctg cgccggggtg cggcccttgg tccagcgcgc gatgtatcga
     2461 ctcatgccga ttcgtcgccg gggccatggc gacctttcac cgggctgccc gtgcccaggt
     2521 acgtaccggc cgcaattccc gggtgtaagg agatcgcccg tcgaacgact gctctacgga
     2581 gaaggcgagg gaggcatggc aagcgaagct gccgtttccg ctgggcccgc cgcgctgagc
     2641 gcccgtcggg gcgaggcgtt ctaccgagcg ctggtggagg gcatgaatga cggggtgctg
     2701 acgcgcgatg cggccggggt gatcacctac gctagcccgc ggtttcgcga catgctcggc
     2761 tacagcgccg aggaactggt gggcgggaag tcgcagttcg tgctgccgcc cgagcagcgc
     2821 agtcgctggg agcgcggtct cgctgccgcc gcgcagggcc cgcagcgctt cgagcacgac
     2881 ctggtctgca aggacgggcg ccgggttgcg gtcgccgtgt cgcgccggcc ggtgttcgac
     2941 gccgaaggcg tgttccaggg ttcggtggcg ctcgtcagca acattaccga agagcgccgc
     3001 gcgagccggc tgctgcagga ggtagcggag gcgaccgcgc cccacgtcgg cgaggagttc
     3061 tttcgcgccg cggtacgctt cctggcgcag gcgctgcgct gcaagatcgt tttcatcacc
     3121 gaatgcgtcg acttcccgac cacgcgcgtg cgcacggtcg cgcgctgggc gctcgaccgg
     3181 ctggcggaca acgtcgagta cgaccttgcc gacaccgcat gcgaggagac gatcaaggcc
     3241 ggcaaagtgt tctactgcgc gagcggggtc gagagccgct tcgccgccgc gcgcgggctg
     3301 ggcgtcgaca gctacctcgg cctgccgctt ttcggcacgg aggatcacgt catcgggcat
     3361 ctggcgttcg tgcacgatgc gccgatcgac cggcagctgc tcgaaaaccc cgtggtgcga
     3421 atcttcgcct cgcgcgccga ggcggagctg cgccgcaagc gcgccgacga cacgatgctc
     3481 ctcatcggcc gggcggtggc gccgctctcc ggcgaggtct ttttccgcac gctgatcgag
     3541 cagctcgtgc gcaccttcgg tttccggcag gcgttcattt ccgagtgcct cgactacccg
     3601 ccgacgcgcg tgcgcacggt agcctactgg gacggcaacg gcctgcgcga gaacacggag
     3661 ttcgatctcg ccggcctgcc gtgcgagatc accctgggcg acgcgcgccc gtacttcgtt
     3721 cccgagcggc tcgaggagct ctaccctgcc gaggcggggc gccggcgcgt gagctacctc
     3781 ggattgccga tcttccatgc cgatcgcaag caggtcgtcg gccacctcgc gttcttcgac
     3841 gagcgcccgc ggccgggcaa cgtgctcgac agcccggcgt ttcgcatcat cgcttcgcgc
     3901 gccggcgtgg agctgctgcg caagcgcgcg gaggacgagc tgcgcgagag cgaggcgaag
     3961 taccggctgc tggtccagaa ccagaccgat ctcctggtca agctggaccg cgaacggcgc
     4021 tatatcttcg tcagcccgtc gttctgcaaa tgcttcggca agaatgagcg cgagctgctc
     4081 ggccggccgt tcgacctcga agtgaatccg ctcgaccgcg gggcgttcga gcaagcgcat
     4141 gccgcggtgc tcgcgccgcc gtatcgcagc cgctgcgagg cgcgggtgct ctcggccaag
     4201 ggatggcagt ggctcgcctg ggaatgctcc gctattctcg acgagcgcgg cgaggtgggc
     4261 gagatcatcg cctccggccg ggacgtcacc gagcgcaggc gcgcggagga gcaggcgcgg
     4321 cagcacctgc agcagcttgc tcacgtcacg cgcgtggcgt ccatgggcga gatggcgagc
     4381 gcgatcgcgc acgagatcaa ccagccgctc gcggcgatca ccacctacac ccaggcctgc
     4441 gtgcgcctgc tgcgctcggg tagcgcgtcg ctcgacgaga tcatcgagac gatggagcgg
     4501 gtcggcgcgc gcgccgagcg cgcgagcgag gtcatccggc acctgcgctc tttcgtgcgc
     4561 aaggaggata cgcagctgat cccggtgcag atcaacttcc tcgtgggcga gatcgtgcgg
     4621 ctggtgcagc ccgaagcggc gcaatccggg gtcgagatca cgaccgacct cggcgagggg
     4681 ttgccggccg tgatggccga caacatccag atcgagcagg tcctgctcaa cctggtgcgc
     4741 aacgcgatcg acgcgatctg cgcggcgcgc atggagcggc gcgaggtccg gatcgtcacg
     4801 cgcctcagcg ccgacggcgc ggtcgcggcc agcgtgcacg acagcggccc gggattcgac
     4861 ggcgagaccg cgggccggtt gttcgagccg tttttcacca ccaaggtcca tgggatgggc
     4921 atcggcctgt cgatcagcca atcgatcgtg gaggcgcacc acggccgcat ctgggccacc
     4981 ggcgagcccg gccgaggcgc gagcttctac gtcgcgctgc ccgcggcgag cgcaggctaa
     5041 gcatggcggc ccagtcgccc gagcccacgg tcttggtggt cgacgatgat gccgagatgc
     5101 gcggcgcgct gagcctcctc atgaagtcga tcgcgctgcg cacgctcacc tttccgaccc
     5161 cgcaggcgtt cctcgctgcc taccagcgcg accatctgcc gg
//
LOCUS       DASUNT010000357         2059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_664046, whole genome shotgun sequence.
ACCESSION   DASUNT010000357 DASUNT010000000
VERSION     DASUNT010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664046"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1477)
                     /locus_tag="VFX94_07250"
     CDS             complement(68..1477)
                     /locus_tag="VFX94_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX5768015.1"
                     /translation="MKPSRNKPKLKKLFVVDTNVLMHDPTSLFRFDEHDLYLPIATLE
                     ELDQHKRGLSDVSRNARQASRFLDEIVSGELESIKNGLALKIRDGQQSKGRLFLQTEA
                     INGELPATLASGRTDNQILAVVRALQEKHPEREVVLVSKDINMRIKARALGLAAEDYT
                     NDKVLEDADLLYTGVRALPRDFWDTHGKDMESWKKDGHTYYRVRGPLVPKLHVNEFVY
                     DESGERPLYALVKEAAGRMAVLETLRDYTHAKNSAWGITARNREQNFALNLLMNPAID
                     FVTLLGQAGTGKTLLALAAGLTQVLDEKRYSEIIMTRVTVPLGEDIGFLPGTEEEKMQ
                     PWMGALEDNLDVLNASDESGGEWGRAATRDLVRSRIKIKSLNFMRGRTFVNKWLIIDE
                     AQNLTPKQMKTLVTRAGPGTKVVCLGNIAQIDTPYLTEGSSGLTYVVDRMKDWGHAGH
                     VTLARGERSRLADHAADVL"
     gene            complement(1518..1970)
                     /locus_tag="VFX94_07255"
     CDS             complement(1518..1970)
                     /locus_tag="VFX94_07255"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5768016.1"
                     /translation="MLGKPVPDFSLPSTGGTFRLSSARGSKLVLYFYPKDDTPGCTSE
                     GADFRDSYSEFRKAGAALFGISRDSLGSHAAFKAKMKFPFELLSDADETACELFGVIK
                     LKNMYGKKVRGIERSTFVVDAQGVLAREWRGVKVPGHVQEVLNFVEAL"
BASE COUNT          324 a          672 c          662 g          401 t
ORIGIN      
        1 ggcagcgaaa tgtagcagaa acgcccggcc ggatggcgaa acaccatccg gcgcgggtta
       61 ttccgtgcta cagcacgtcg gccgcgtgat cggcgaggcg cgagcgctcg ccgcgcgcga
      121 gcgtgacgtg gccggcgtgc ccccagtcct tcatgcggtc caccacgtag gtgaggccgg
      181 aggagccttc ggtgaggtag ggcgtatcga tctgcgcgat gtttcccagg cacaccacct
      241 tggtgcccgg accggcgcgc gtgaccagcg tcttcatctg cttcggcgtc aggttctgcg
      301 cctcgtcgat gatcagccac ttgttgacga aggtgcggcc gcgcatgaag ttgagcgatt
      361 ttatttttat cctggatcgg accagatcgc gcgtcgctgc ccggccccat tcaccgcccg
      421 attcgtcgga agcgttgagg acatcgaggt tgtcctcgag cgcgcccatc cagggctgca
      481 tcttctcctc ctcggtgccc ggcagaaagc cgatgtcctc gccgagcggc acggtgacgc
      541 gcgtcatgat gatctcggaa tagcgcttct cgtccagcac ctgggtgagg ccggcggcga
      601 gcgcgagcag cgtcttgccg gtgccggcct ggccgagcag cgtgacgaag tcgatcgccg
      661 ggttcatgag caggttgagc gcgaagttct gctcgcgatt gcgcgcggtg atgccccacg
      721 ccgagttctt ggcgtgcgtg tagtcccgca acgtctcgag taccgccatg cggcccgccg
      781 cctccttcac cagcgcgtag agtggccgct cgcccgactc gtcgtacacg aactcgttca
      841 cgtgcagctt cggcacgagc ggcccgcgca cgcggtagta cgtatggccg tccttcttcc
      901 aggactccat gtccttgccg tgcgtgtccc agaagtcgcg cggcagcgcg cgcacgccgg
      961 tgtagaggag atcggcgtcc tcgagcacct tgtcgttggt gtaatcctcc gccgcgagcc
     1021 cgagcgcacg cgccttgatg cgcatgttga tgtccttcga caccagcacc acctcgcgct
     1081 cgggatgctt ctcctgcagc gcgcgcacca cggcgaggat ctggttgtcg gtgcggcccg
     1141 aagcgagcgt cgcgggcagc tcgccgttga tcgcctcggt ctgcaggaag agccggccct
     1201 tcgactgctg gccgtccctg atcttgagcg ccaggccgtt cttgatcgat tcgagctcgc
     1261 cgctcacgat ctcgtcgagg aagcggctcg cctgccgggc gttgcgcgac acatccgaca
     1321 gcccgcgctt gtgctggtcg agctcctcga gcgtcgcgat cggcaggtag aggtcatgct
     1381 cgtcgaagcg gaagaggctc gtcgggtcgt gcatcagcac gttcgtgtcg accacgaata
     1441 gcttcttgag cttgggcttg ttgcgggacg gcttcacggc ggcgacggac tctagctcta
     1501 ctgcgggcgg ctcgacatca tagggcttca acaaagttca atacctcctg gacgtggccc
     1561 ggcaccttga ccccgcgcca ctcgcgcgcg agcaccccct gcgcgtccac cacaaaggtg
     1621 ctgcgctcga tgccgcggac cttttttccg tacatgttct tcaacttgat caccccgaac
     1681 agctcgcagg cggtctcgtc cgcgtccgag agcagctcga acgggaactt catctttgcc
     1741 ttgaaggctg cgtgcgaccc gagcgagtcg cgcgagatgc cgaacagcgc cgcgcccgcc
     1801 ttcctgaact cgctgtacga atccctgaag tccgcgccct cgctggtgca gcccggggtg
     1861 tcgtccttcg gatagaagta aaggacgagc ttgctgccgc gagcgctgga gagcctgaag
     1921 gtgccgccgg tactgggaag gctgaagtcc gggaccggct tgccgagcat ttccagaggt
     1981 tttgagtacc cttcttcgta aagcgctgta acgcctgcat tgaacacaag tcgggtttgc
     2041 ggcgcaagcg cgatacgtg
//
LOCUS       DASUNT010000358         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_926747, whole genome shotgun sequence.
ACCESSION   DASUNT010000358 DASUNT010000000
VERSION     DASUNT010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926747"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VFX94_07260"
     CDS             <1..1071
                     /locus_tag="VFX94_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5768017.1"
                     /translation="AVRPQDLAYLLYTSGSTSLPKGVEIQHYALIENMWHIGERQKVT
                     ERDRLWLAVSLFWGLGCENALFNLFTHGGCVVLQEHFDAAEALELIERERCTVFYGTP
                     NMAQALYEHPDRGKRDLSSLRGGATIGTPEQIMRVVELGAREVCNIYGLTETYGNCNV
                     TDARDPLEKRLANVGRPLPGVEQRIVHPEAGKVLPPGEVGEIRVKGYVTTGYYKDPER
                     TREAFDEHGFFRTGDLGLHDAEGYLHFRGRIKEMVKTGGINVAPIEVEEVLLSHPAVQ
                     AAFVTGVPDAKHDELLAAVIVPKRGASLTEREVTDHCRASLAAYKIPRLIRFAAEEEL
                     PLTTTGKVQKNRLASTFFARGQ"
     gene            1256..1664
                     /locus_tag="VFX94_07265"
                     /pseudo
     CDS             1256..1664
                     /locus_tag="VFX94_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632246.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1679..>2264)
                     /locus_tag="VFX94_07270"
     CDS             complement(1679..>2264)
                     /locus_tag="VFX94_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX5768018.1"
                     /translation="PSEGLRALDIDPDRVEDVIITHMHYDHAGNHHLFQRARFHFQDR
                     EMFYVTGRCMTHAVLGHSYDVEDVVQMVRRVFSRRAVFHEGDEELAPGLSVHFVGGHT
                     MGLQMVRVWTQRGWVVLASDAAHYYANIEQSRPFPVVYNVGDMLEGHKRAYALADSPR
                     HLVPGHDPLVLKRYPAPRRGLEGIVARLDVEPRE"
BASE COUNT          416 a          758 c          722 g          368 t
ORIGIN      
        1 gccgtgcggc cgcaggactt ggcctacctc ctttacacct cgggctctac atcacttccc
       61 aagggcgtag agatccagca ctacgcgctc atcgaaaaca tgtggcacat cggcgagcgc
      121 cagaaagtga ccgagcgcga ccgtctctgg ctggcggtgt cgctcttctg gggtctcggc
      181 tgcgaaaacg cgcttttcaa cctcttcacc cacggcggct gcgtggtgct ccaggagcac
      241 ttcgacgctg cagaggcgct cgagctcatc gagcgcgagc gctgcaccgt cttctacggc
      301 accccgaaca tggcgcaggc gctctacgag catcccgacc ggggaaagcg cgacctttcg
      361 agcctgcgcg gcggcgcgac catcggaacc cccgagcaga tcatgcgtgt ggtggagctc
      421 ggcgcgcgcg aggtctgcaa catctacggc ctcaccgaga cctacggcaa ctgcaacgtc
      481 accgacgcgc gcgacccgct cgagaagcgc ctcgcgaacg tcggccgccc gctccccggc
      541 gtcgagcagc gcatcgtcca cccggaagcg ggaaaggtgc tgccgcccgg cgaagtcggc
      601 gagatccgcg tcaaaggcta cgtcaccacc ggctactaca aggaccccga gcgcacccgc
      661 gaggccttcg acgagcacgg cttcttccgc accggcgacc taggcctcca cgacgccgag
      721 ggctacctcc acttccgcgg ccgcatcaag gagatggtca agaccggcgg catcaacgtc
      781 gccccgatcg aggtggaaga ggtcctgctc tcccatcccg ccgttcaggc cgccttcgtc
      841 actggcgtcc cggacgcgaa gcacgacgag ctcctcgccg ccgtcatcgt ccccaagcgc
      901 ggcgcctccc tcaccgagcg cgaggtcacc gaccactgcc gcgcgtccct cgccgcctac
      961 aagatccccc gcctcatccg cttcgccgcc gaggaagagc tccccctcac caccaccggc
     1021 aaggttcaaa agaaccggct cgcgagcact ttttttgcaa gaggtcagta gagcccttat
     1081 accggcctcg gccagagcgc cgcttgcgcg tttgggcgcg cctgcttgct catgcagcgc
     1141 acgggcaagg gacttcgaag cgcgcctctt ttctgcaatt ggatgtcgat tccatatgcc
     1201 cctgttcgac aagatcaggc gggcaagtcc tcttaactct tataggagta tggaaatggc
     1261 caagaaaaac acgatctgcc tgtggtacga cggcaccgct ttggacgccg ctcggttcta
     1321 tgccgagacc ttccccgaca gcgccgtagg cggtaggcat tccctgcatc ggactcaacg
     1381 gcgggccgca gttcaagcac aacgaggcgt tctcattcca gatcgcgacc gacgatcagg
     1441 ccgaaaccga tcgcttgtgg cacgcgattg taggaaacgg gggcgaggaa agcgcgtgcg
     1501 ggtggtgtaa agacaagtgg ggcctgaact ggcagattac gccacgcgtc ctcatcgatg
     1561 cgatctccga ccctgatcgc gcggcggcca agcgtgcgtt tgaagcgatg atgaagatga
     1621 gaaaaatcga catcgccgcg atcgaggcgg cgcggcgggg ctgaaaagcg aggtggattc
     1681 actctcgcgg ctcgacgtca aggcgcgcga cgatgccttc gaggccgcgg cgtggcgcgg
     1741 ggtagcgctt gagcaccagc gggtcgtggc cagggactag gtggcgcgga gagtcggcga
     1801 gcgcgtaggc gcgcttgtgg ccttcgagca tgtcgccgac gttgtagacc accggaaacg
     1861 ggcgcgactg ttcgatgttg gcgtagtagt gcgccgcgtc ggaggcgagc accacccagc
     1921 cacgctgggt ccacacgcgc accatctgca gtcccatggt gtggccgccg acgaagtgca
     1981 cgctcaagcc tggcgcgagc tcctcgtcgc cctcgtggaa cactgcgcgg cggctgaaca
     2041 cgcggcgcac catctgcacc acgtcctcga cgtcgtagga gtgcccgagc accgcgtggg
     2101 tcatgcagcg gccggtgacg tagaacatct ccctgtcctg gaaatggaag cgcgcacgct
     2161 ggaagaggtg gtggttgccg gcgtggtcgt aatgcatgtg ggtgatgatc acgtcctcga
     2221 cccggtcggg gtcgatgtcg agggcgcgca ggccctcgct cggg
//
LOCUS       DASUNT010000359         2961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_402072, whole genome shotgun sequence.
ACCESSION   DASUNT010000359 DASUNT010000000
VERSION     DASUNT010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402072"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFX94_07275"
     CDS             <1..279
                     /locus_tag="VFX94_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX5768019.1"
                     /translation="AKDVEDVLYRHPQVKAAGVIGVPDPAVGQRIKAIVVLHGDARGK
                     LSEDEIKTYCRQSLAEYKVPHIVEFRGELPKTDVGKVSRRELRDETKV"
     gene            282..1055
                     /locus_tag="VFX94_07280"
     CDS             282..1055
                     /locus_tag="VFX94_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5768020.1"
                     /translation="MASAFFAVEALTKRFGGLTAVNKVSFSLARDEIVGLIGPNGAGK
                     TTLLRLITKILKPDSGRIVLNGEDITGLRPWDVVERGIAGTFQNTRPFRHLPIVANVM
                     VPLVAPRARRGGEWVKRIEAKAMDALEFVGISDMALEPASALSQGDLKRLEVARAIAT
                     EPELLLLDEPLGGLNPAESELLAKSIRRLHKGGRFGRLHSEGPAVLMIEHKLKELMSI
                     VDRVIVIDYGEIIADGPPAEVVKNPKVIEAYLGSEHAAA"
     gene            1042..1791
                     /locus_tag="VFX94_07285"
     CDS             1042..1791
                     /locus_tag="VFX94_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5768021.1"
                     /translation="MPLLEVRNLRVRYDKAQILNGVTLSVNEGELVGLVGPNGAGKST
                     LLRAISGLVRFEERMKRGTTGDIVLEGEVRFAGERIDGLPAHEIRMRGLVHCPERRRP
                     FRELTVQENLLAGAFLSRSKEETQRRLDHCFSLFPRLSERKTQIAGTLSGGEQQMLAT
                     ARALMFEAKLLVIDEPSLGLAPRIREELFAAVGKIHAEGVPVLLVEQEVAQVFRMARR
                     NYVLSQGRIIAEGSAQSLMADETLRAGYLGL"
     gene            complement(1744..2820)
                     /locus_tag="VFX94_07290"
     CDS             complement(1744..2820)
                     /locus_tag="VFX94_07290"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5768022.1"
                     /translation="MYWLKRGWAELRNEVLVLPSRTLVLLWVAALLLLPVVYSDAYVL
                     RIVTMTCIFAIFAASWDLLAGYTGQVNFGHALFFGAGAYTSALLSLKLGLSPWATVWA
                     GAAVALLFGFVVGYLCLRLRGSYLSLATLAFPLIALGVLFAFPDFSGGELGISGLRRL
                     AVTPTSNYYVAAISMLFIVFALWIFADSKFGIVLHAIRDDEVAARASGINTLRYKLIV
                     FAISGAAAGFAGALFAHYLRVAGPSTLEVALSFQVVIWGIFGGVATVYGPVAAVFILY
                     PLTEWLGSFRAFGELRLLIFAIIVLLVLLFMPRGLTPWVRDKIETQCPRCKQRNSSWK
                     KICRLCGAGLEAQVPGAQRLVGHQ"
BASE COUNT          579 a          953 c         1010 g          419 t
ORIGIN      
        1 gcgaaggacg tcgaggacgt gctctaccgc cacccgcagg tgaaggcggc gggcgtgatc
       61 ggcgtgcccg acccggcggt cgggcagcgc atcaaggcga tcgtggtcct gcacggcgac
      121 gcccgcggca agctctcgga ggacgagatc aagacgtatt gccggcagag ccttgccgag
      181 tacaaggtgc cgcacatcgt cgagtttcgc ggcgagctgc cgaagacgga tgtcggcaag
      241 gtctcgcgcc gcgagctgcg cgacgagacg aaggtctagg cttggcgtca gcgttcttcg
      301 ccgtcgaggc gctcaccaaa cgcttcggcg gccttaccgc agtcaacaag gtcagcttct
      361 cgcttgcgcg cgacgagatt gtgggcctca tcgggccgaa cggcgcgggc aagacgacgc
      421 tgctgcgcct gatcaccaag atcctcaagc cggactccgg gcgcatcgta ttgaacggcg
      481 aggacatcac cggtctgcgt ccctgggacg tggtcgagcg cggcatcgcg ggcacgttcc
      541 agaacacgcg gccgttccgc cacctgccga tcgtcgcgaa tgtgatggtg ccgctggtgg
      601 cgccgcgcgc gcgtcgcggc ggcgaatggg tcaagcgcat cgaggcgaaa gcgatggacg
      661 cgctcgagtt cgtcggcatc tcggacatgg cgctcgagcc ggcctcggcg ctatcgcaag
      721 gcgacttgaa gcgcctggag gtcgcgcgcg ccatcgccac cgagcccgag ctgctgctgc
      781 tcgacgagcc gctcggcggc ctcaacccgg ccgagtccga gctgctcgcg aaatcgatcc
      841 gccgcctgca caagggcgga cgcttcggca ggctgcattc cgagggcccg gcggtgctga
      901 tgatcgagca caagctgaag gagctgatgt cgatcgtcga ccgggtaatc gtgatcgact
      961 acggcgagat catcgccgac gggccgccgg ccgaggtggt gaaaaatccg aaagtaatcg
     1021 aagcctacct ggggagcgag catgccgctg cttgaggtga ggaacctgcg cgtgcgctat
     1081 gacaaagcgc agatcctgaa cggcgtgacg ctctcggtga acgaaggcga gctagtcggc
     1141 ctggtggggc cgaacggcgc cggcaagtcc acgctgctgc gcgcgatctc gggcctggtg
     1201 cgtttcgagg agcgcatgaa gcgcggcacc accggcgaca tcgtgctcga gggcgaagtg
     1261 cgcttcgccg gcgagcgtat cgacggcctc cccgcacacg agattcgcat gcgcggcctg
     1321 gtgcactgcc cggagcgccg gcgcccgttc cgcgagctta cggtccagga gaacctgctc
     1381 gccggcgcct ttctttcaag aagcaaggaa gagacgcaac gtcgtctcga tcactgcttt
     1441 tcgctcttcc ctcgtctgtc ggaaaggaag acgcagattg cgggaacgtt gtcggggggc
     1501 gaacagcaga tgctcgccac ggcacgggcg ctcatgttcg aggcgaagtt gctcgtgatc
     1561 gacgaaccct cgctcgggct cgcgccgcgc atccgcgagg agctgtttgc cgcggtcggc
     1621 aagatccacg ccgagggcgt gccggtcctt ctggtcgagc aagaagtcgc gcaggtgttc
     1681 cggatggcaa gacggaacta cgtgctgtcc cagggccgta tcattgccga gggctcggcg
     1741 cagtcactga tggccgacga gacgctgcgc gccgggtacc tgggcctcta gcccggcgcc
     1801 acacaatcgg caaatcttct tccacgaaga atttctttgt ttgcagcgcg ggcattgcgt
     1861 ctcgatcttg tcgcgcaccc acggcgtgag cccgcgcggc atgaaaagca gcaccagcag
     1921 gacgatgatg gcaaagatca gcaggcgcag ctcgccgaag gcccggaagc tgccgagcca
     1981 ctcggtcagc ggatagagga tgaacaccgc cgcgaccggg ccgtagacgg tcgcgacgcc
     2041 gccgaagata ccccagatca ccacctggaa ggaaagcgcg acctcgagcg ttgaaggacc
     2101 cgcgacccgc aggtaatgcg caaacagtgc gccggcgaac cccgccgccg cgcccgagat
     2161 cgcgaacacg atcagcttgt agcgcagggt gttgataccg gaggcgcgcg cggcgacttc
     2221 gtcgtcgcgg atggcgtgca acacgattcc gaacttggag tcggcgaaga tccacagcgc
     2281 aaagacgatg aagagcatcg agatcgccgc cacgtagtag ttgctggtcg gcgttacggc
     2341 aaggcggcgc aatccggaga tgccgagctc gcccccggag aagtccggga aggcgaacag
     2401 cacgccgagc gcgatcagcg ggaacgcgag cgtggcgagc gaaagatacg agccgcgcag
     2461 ccgcaggcac agatagccga cgacgaagcc gaacagaagc gcaaccgccg cccccgccca
     2521 gaccgtcgcc cacggcgaga gcccgagctt gagggagagc agcgccgagg tgtaggcgcc
     2581 ggcaccgaag aacagcgcgt ggccgaagtt cacctgcccg gtatagccgg cgagcaggtc
     2641 ccagctcgcc gcgaagatcg cgaagatgca cgtcatcgtg acgatgcgca gaacgtaggc
     2701 gtcgctatac accaccggca gcaggagcag cgccgcaacc cacaacagga cgagggtgcg
     2761 cgatggcagc acgaggactt cgttgcgcag ctcggcccac ccgcgcttaa gccaatacat
     2821 cagcgttcct cctcgaacgc caccccgaac aggccctcgg gcctggcgag aagcacggcg
     2881 accatgatca tcagggcgac ggcgcccttg aggtacgccc cggccggcac cgcaaaaacc
     2941 gtgatcgcct cgacgtagcc g
//
LOCUS       DASUNT010000360         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1279085, whole genome shotgun sequence.
ACCESSION   DASUNT010000360 DASUNT010000000
VERSION     DASUNT010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279085"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="VFX94_07295"
     CDS             complement(<1..1106)
                     /locus_tag="VFX94_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX5768023.1"
                     /translation="MLIGSVGLYLLLTIAIGLWAAQRVHNAKDYVVAGRSLPLYMSAA
                     TVFATWFGAETVLSVSATFTRDGLGGIIADPFGASFCLLFVALFFARAFYRMDLLTIG
                     DFYHKRYNKTVEVITSVAITASYLGWTSAQLTALGLVFYVLSGGAISLTTGILIGAGV
                     VVVYTIFGGMWSVALTDLFQSVVILIGLVAVAWLVGDMAGGAGKVIAAASEAGKFEFW
                     PKGGTKEWLAFLAAWMTLAIGSIPQQDVFQRVTSAKDEKTAVRGTLIGGAVYFCFAFV
                     PIFIAYSALVIDPGYEKLFANEDAREIQRILPDLILNKTPMWAQILFFGALLSAILST
                     ASGALLAPTALFTENVMRPFMRNVSDRQFLVLLRT"
     gene            complement(1117..1707)
                     /locus_tag="VFX94_07300"
     CDS             complement(1117..1707)
                     /locus_tag="VFX94_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5768024.1"
                     /translation="MPSDSSAPQFWDTRFRSGVTPWDAGAAPARLASWIRTRPSGSKV
                     LVPGCGTGYEVALFARHGDDVLGIDFSDAALEAARRYLGAQSRLVRQEDFFSFGAGPF
                     DLVYERAFLCALPRARWSNWARRMSELVRPGGELAGFFYLDDNERGPPFGTSREELHR
                     LLERAFVLREDEAIAPRDSLAVFAGKEIWQLRTRRL"
     gene            complement(1694..>2627)
                     /locus_tag="VFX94_07305"
     CDS             complement(1694..>2627)
                     /locus_tag="VFX94_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX5768025.1"
                     /translation="EELDLMREAGNASELRRNFEASPLLVVPEVHWDLCAQRVMVMER
                     MHGTPISQVQALREKGVDIPALARAGVEIFFTQVFRDGFFHADMHPGNILVTDDGRYV
                     ALDFGIMGTLTEVDKNYLAQNFLAFFNRDYRRVAQAHLDAGWVPAGTRIDAFEAAIRA
                     VCEPVFARPLKEIYFGKLLLRLFQTSRRFNVEVQPQLLMLQKTLLNIEGLGRELDPDL
                     DLWQTAKPFLERWMREQVGWRGLVRTIRHEAPFWAATLPQLPRLVHRALADDRLGGLQ
                     LALEHIARQNGRRNRLLAGLLAAIVLGFVVLALR"
BASE COUNT          495 a          869 c          898 g          365 t
ORIGIN      
        1 gtgcgcagca gcaccaggaa ctgccggtcg gacacgttgc gcatgaacgg ccgcatcacg
       61 ttctcggtga acagcgccgt cggcgcgagc agcgcgccgc tcgcggtcga gaggatggcc
      121 gagagcagcg cgccgaaaaa caggatctgc gcccacatcg gggtcttgtt gaggatcagg
      181 tcgggcagga tgcgctggat ctcgcgcgcg tcctcgttcg cgaagagctt ctcgtagccc
      241 gggtcgatca cgagcgccga gtaggcgatg aagatcggca cgaaggcgaa gcagaagtac
      301 accgcgccgc cgatcagcgt gccgcgcacc gcggtctttt cgtccttggc cgaagtgacg
      361 cgctggaaca cgtcctgctg cgggatggag ccgatggcga gcgtcatcca ggcggcaagg
      421 aatgcaagcc attccttggt gccgcctttc ggccagaact cgaacttgcc cgcctcggag
      481 gcggcggcga ttaccttgcc ggcaccgccc gccatgtcgc cgacgagcca tgccaccgcc
      541 accaggccga tcaggatgac aacggactgg aagaggtcgg tgagcgcgac cgaccacatg
      601 ccgccgaaga tcgtgtagac gaccaccacg ccggcgccga tgagaatgcc ggtggtgagg
      661 ctgatcgcgc cgccggaaag cacgtagaac accaggccga gcgcagtgag ctgggccgaa
      721 gtccagccca ggtacgaagc cgtgatcgcc acgctggtga tcacctcgac cgtcttgttg
      781 tagcgcttgt ggtagaagtc gccgatcgtg aggaggtcca tgcggtaaaa cgcacgggcg
      841 aagaagagcg ccacgaaaag caggcagaat gaggcgccga agggatcggc gatgatccct
      901 cccaggccgt cccgagtgaa tgtcgcggac accgagagca cggtttcggc gccgaaccag
      961 gtagcgaaca cggtggccgc gctcatgtac agcggcaggc tgcggccagc cacgacatag
     1021 tccttggcgt tgtggacccg ctgcgcggcc cacagcccaa tggcgatagt aagcagcagg
     1081 tagagcccga ccgaaccgat cagcatgcgc gcgccatcat agccgccgcg tccgaagctg
     1141 ccaaatctcc ttgccggcga aaaccgcgag cgagtcgcgg ggcgcaatcg cctcgtcctc
     1201 gcgcaagaca aacgcccgtt cgagcagccg atgcagttct tcccgcgagg tgccgaaagg
     1261 cgggccgcgc tcgttgtcgt cgaggtagaa aaagccggcg agctccccgc ccgggcggac
     1321 gagctcgctc atccggcgcg cccagttgga ccagcgcgcg cgcggcaatg cgcacaggaa
     1381 ggcgcgctca tacaccaggt cgaacggccc cgcgccgaac gaaaagaaat cctcctgcct
     1441 caccagccgc gattgcgctc cgaggtaacg gcgcgccgct tcgagcgcgg catccgagaa
     1501 atcgatgccg agcacatcgt cgccatggcg cgcgaagagc gcgacctcgt agccggtgcc
     1561 gcagccgggg acgagcacct tcgagcccga cggccgggtg cgtatccacg acgccaggcg
     1621 cgcgggcgcg gcgccggcat cccagggcgt cacgccgctt cggaaacgcg tgtcccagaa
     1681 ctgcggcgcg gagctatcgg agggcaagga cgacgaagcc gaggacgatg gccgcgagga
     1741 gccccgccag gaggcggttg cgccgcccgt tttgccgcgc gatgtgctcg agagcgagct
     1801 gcaggccgcc caggcgatcg tcggccagcg cgcgatgcac gagccgcggc agctgcggca
     1861 gcgtcgccgc ccagaacggc gcctcgtggc ggatggtgcg caccagcccg cgccagccga
     1921 cctgctcgcg catccagcgc tcgaggaagg gcttggcggt ctgccagaga tccaggtccg
     1981 ggtcgagctc gcgccccagt ccctcgatgt tgagcagggt cttctgcagc atcagaagct
     2041 gcggctgcac ctcgacgttg aagcgtcgcg aggtctggaa caggcgcaag agcagcttgc
     2101 cgaagtagat ctccttgagc ggccgggcga atacgggctc gcacaccgcg cggatcgcgg
     2161 cctcgaacgc gtcgatgcga gtgcccgcgg gcacccagcc cgcatcgagg tgcgcctgcg
     2221 ccacgcgccg gtagtcgcgg ttgaagaagg cgaggaagtt ctgcgcgagg tagttcttgt
     2281 ccacctcggt gagcgtgccc atgatgccga agtcgagcgc cacgtatctg ccgtcatccg
     2341 ttaccagaat attcccggga tgcatgtcgg catggaagaa gccgtcgcgg aatacctggg
     2401 tgaagaagat ctccacgccg gcgcgggcaa gggcggggat gtcgacgccc ttctcccgca
     2461 gcgcctgcac ctgcgagatc ggcgtgccgt gcatgcgctc catcaccatg acgcgctgcg
     2521 cgcaaaggtc ccagtgcact tcgggcacca cgagcagcgg cgaggcttcg aagttgcgcc
     2581 gcagctcact cgcgttgccg gcctcgcgca tcaggtccag ctcctct
//
LOCUS       DASUNT010000361         5108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1190796, whole genome shotgun sequence.
ACCESSION   DASUNT010000361 DASUNT010000000
VERSION     DASUNT010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190796"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1481)
                     /locus_tag="VFX94_07310"
     CDS             complement(<1..1481)
                     /locus_tag="VFX94_07310"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5768026.1"
                     /translation="MAANSTLRQANRLGRYEILRELGRGAIGVVYAARDKSTGAAVAL
                     KSCDAALLNLPKANFSDLFLKEVRAAWRLKHRNIVNIHDAGEASGTGYVAMELLEGET
                     LRKVLDAGPLSIARAIKIADEIASGLAYAHEQGVVHRGVKPSNIIVLRAGGVRICDFG
                     FGLLAEEAMLSGQREGCLSCMSPEQVGGDPVDARSDIFALGAVFYEMLARRPAFPGDS
                     PHEIMQKIVHAEPPPPSDANPHVPRALDRIVLGMLVKRPDDRLPGAQIVLRELQRLRH
                     ELGLGEQMQAANRLAPHDSAQPAALQPPRDHEPRLRTPDAGRVRGVAPTDHGPRLRDR
                     EAFDQRDDMLLMDHEPEFERSFASRSGVFAWAALVLAVLAIGLAVFLHYSPEPAGRRI
                     TALLQQATGTAPAPPPPSPPQRASETANGAAAQPPAPPPAPQPRAAEPPAPPPPAPEP
                     LPTAPSPTPQAESSVAAAAEPSAAKPSGAAGTTRTQEAPPATKP"
     gene            complement(1585..2418)
                     /locus_tag="VFX94_07315"
     CDS             complement(1585..2418)
                     /locus_tag="VFX94_07315"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX5768027.1"
                     /translation="MNGSTLVSTGELAANLTKWRVFDCRHDLGNPKLGEQQYREGHIP
                     GALFAHLERDLSGRKTGANGRHPLPEPQDFEKWLERTGLTPQDHAVCYDAGPGAMAAR
                     LWWMLRWVGHETVAVLDGGFAKWTKEGRPVSVDVPLFTRFNYPIRARSDMAADVGVVE
                     KSLGTALLLDARAPARYRGEHEPIDPVAGRIKGAKNRFNMDNVSAHGTFKPPQELKKE
                     FQSVLQGRSAAEVIHYCGSGVAACHNLLAMEIAGLAGGKLYAGSWSEWSADPRRPLER
                     G"
     gene            complement(2387..3484)
                     /locus_tag="VFX94_07320"
     CDS             complement(2387..3484)
                     /locus_tag="VFX94_07320"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006027818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX5768028.1"
                     /translation="MADVAKLKQLKPASCQLPVSWYFDPHVFEAEKRLVFQAGPTYVG
                     HELMVPNAGDWHTLAWMDHAKVLVRNASGVELLSNVCRHRQAIMLQGRGNAENIVCPV
                     HRWTYDMKGALLGAPRFADQPCVKLASTPLKSWKGLLFAGPRDPAADLSRITTLADWD
                     FSGFVLDSVRIDEYDINWKTFIETYLEVYHVDFFHPGLGNFTDCDNFTVDYGPDYSVQ
                     MVAAKSGLARPGTPTYKRWHEACLKYLDGKAPKYGALWMTYYPGLMLEWYPHVLVISH
                     LIPRTPTRTTNVVEFYYPEEIALFEREFIEAQQAAYVETAMEDNEICLRLDRGRRALY
                     EQGLDDAGPYQSPMEDAEVHFHEWLHARLDR"
     gene            3525..3773
                     /locus_tag="VFX94_07325"
     CDS             3525..3773
                     /locus_tag="VFX94_07325"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX5768029.1"
                     /translation="MSHPSAEPRQLDFEKALAELEKIVARMESGELSLEQALATHKRG
                     IELARFCQQKLEAAQQQVKVLEGEALKPLAAGEPDEDS"
     gene            3814..4650
                     /locus_tag="VFX94_07330"
                     /pseudo
     CDS             3814..4650
                     /locus_tag="VFX94_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
     gene            4662..>5108
                     /locus_tag="VFX94_07335"
     CDS             4662..>5108
                     /locus_tag="VFX94_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEX5768030.1"
                     /translation="MAAISHELPADGGEPASYALLRTIDDPAQLRALDRRQLGQLAEE
                     LRAFVLESVSKTGGHLSSNLGTVELTIALHYVFDTPEDRIVWDVGHQTYPHKILTGRR
                     DRMKTLRQYGGLSGFCLRSESEYDVVAAGHASTSISAALGLAVARDL"
BASE COUNT          829 a         1654 c         1772 g          853 t
ORIGIN      
        1 ggtttcgttg ctggcggagc ctcctgcgtt cgcgtcgtgc cggctgctcc ggatggcttg
       61 gcggcagagg gctcagccgc agcagcaacc gagctctcgg cttgcggggt tgggctgggc
      121 gccgtcggga gcggctccgg cgctgggggc ggcggcgcgg gcggctcagc ggcgcgcggc
      181 tgcggcgcgg gcggtggcgc gggaggctga gccgcagctc cgttcgccgt ctcggaagcg
      241 cgctgaggcg ggctcggggg aggcggcgcg ggtgcggtcc cggttgcttg ttgcagaagc
      301 gcggtgatgc gtcgcccggc cggctctggc gagtaatgca agaagacggc aagtccaatc
      361 gccagcacgg cgagcaccag agcggcccac gcgaacacgc cagaccgcga cgcaaaggac
      421 cgttcgaact cgggttcgtg gtccatcagg agcatatcgt cgcgctggtc gaacgcctcg
      481 cgatcgcgta accggggccc atggtctgtg ggcgcaacgc cccggacccg gcccgcgtcc
      541 ggcgtgcgca gccgcggctc gtgatcgcgc ggcggctgga gtgccgcagg ctgcgccgaa
      601 tcgtgtggcg ccaagcgatt tgctgcctgc atttgctccc ccaggccaag ctcgtgcctg
      661 aggcgctgca gctcgcgcag cacgatctgc gcgccgggca agcggtcatc cggacgcttg
      721 acgagcatgc cgagaacaat gcggtcgagc gcacgtggaa cgtgcggatt cgcatcgctg
      781 ggcggcggcg gctcggcatg aacaattttc tgcatgattt cgtgcggcga gtcgcccggg
      841 aaggctggcc gccgagcgag catctcgtag aaaaccgcgc cgagcgcgaa aatgtcggac
      901 cgggcgtcga ctggatcgcc gcccacctgc tcgggcgaca tgcagctcag acagccctcc
      961 cgctgtcccg aaagcatcgc ttcctcggca agcagcccga agccgaaatc gcagattctc
     1021 acgccgcccg cgcgcaggac gatgatgttg gagggcttga cgccccggtg cacgacgcct
     1081 tgttcgtgtg catacgcgag cccggatgcg atttcatcgg cgatcttaat cgcgcgcgcg
     1141 atggagagcg ggccggcgtc gaggaccttg cgcagggtct cgccctcgag gagctccatc
     1201 gccacgtagc ccgtgccgct cgcttcgccg gcatcgtgaa tgttcacgat attgcggtgc
     1261 ttgagccgcc acgccgcgcg cacctccttc aggaacaagt cggagaagtt ggccttcggc
     1321 aggttcaata acgcggcatc gcagctcttc aatgcaacgg ccgcgcccgt ggacttgtcg
     1381 cgcgccgcgt agaccacgcc gatcgcgccg cgtcccagct cgcgaagaat ttcgtagcgc
     1441 ccgagcctgt tcgcctgcct cagcgtcgaa ttagcggcca ttgcctggat ggaagtgtgg
     1501 gatacatgaa cctggacaag cttacgcgcc gcctatcgct tggttcaaga tgaagcagcg
     1561 agtcgtcgcg aatcgacgac aatcctagcc gcgctcaagc ggccgccgcg gatcggcgct
     1621 ccactcgctc cacgagccgg cgtagagctt gccgcccgcg agccccgcga tctccatcgc
     1681 gagcaggttg tggcacgccg ccacgcccga gccgcagtag tggatgactt ccgcggcaga
     1741 gcgaccttgc aggactgact ggaattcctt cttcagctct tgcgggggct tgaatgttcc
     1801 gtgcgcggaa acgttatcca tgttgaagcg atttttcgcc cccttgatgc ggcccgcgac
     1861 cgggtcgatc ggctcatgtt cgccgcggta gcgggcaggc gcgcgggcgt cgaggagcaa
     1921 cgccgtgccg agagatttct ccactacgcc gacatccgcg gccatgtcgc ttcttgcacg
     1981 aatcggataa ttgaatcgag tgaagagcgg cacgtcgacg ctgacaggcc ggccttcctt
     2041 cgtccacttg gcgaatccgc cgtcgagcac ggcgactgtc tcgtggccga cccagcgcag
     2101 catccaccac aggcgcgccg ccattgcgcc gggtccggcg tcgtagcaca ccgcatggtc
     2161 ctgcggcgtg aggcctgtcc tttccagcca cttctcgaag tcctgcggct cgggcagggg
     2221 atggcgtccg ttcgcgcccg tcttgcggcc cgagagatcg cgctcgaggt gcgcgaaaag
     2281 cgcgccggga atgtggccct cgcggtactg ctgctcgccg agtttggggt tgccgaggtc
     2341 gtggcggcag tcgaagaccc gccacttggt gaggttggcg gctagctcac cggtcgagac
     2401 gagcgtggag ccattcatgg aagtgcacct ccgcgtcctc catcggcgac tgataggggc
     2461 cggcgtcgtc gaggccctgc tcgtagaggg cgcggcggcc gcggtcgagc cgcaggcaga
     2521 tctcattgtc ctccatcgcc gtctcgacgt aggcggcctg ctgcgcctcg atgaactcgc
     2581 gctcgaagag cgcgatttcc tcgggatagt aaaactcgac cacgttcgtg gtgcgggtcg
     2641 gcgttcgcgg gatgaggtgc gaaatcacca gcacgtgcgg gtaccactcg agcatcaggc
     2701 ccgggtagta cgtcatccag agcgcgccgt acttgggcgc cttgccgtcg aggtacttta
     2761 ggcaggcctc gtgccagcgc ttgtaggtcg gcgtcccggg gcgcgccagg cccgacttgg
     2821 ccgcaaccat ctgcaccgag tagtcgggcc cgtagtcgac ggtgaagttg tcgcagtcgg
     2881 tgaagttgcc gagcccggga tggaagaagt ccacgtggta gacctcgagg tatgtctcga
     2941 tgaaggtctt ccagttgatg tcgtattcgt cgatacgtac cgagtcgagg acgaagccgc
     3001 tgaagtccca gtcggcaagg gtggtgatcc ttgacagatc ggctgccggg tcacgcggcc
     3061 cggcgaagag aaggcccttc cagctcttga gcggcgtgga agcaagcttc acgcacggct
     3121 ggtcggcgaa gcgcggggcg ccgagcagcg cacccttcat gtcgtaggtc cagcggtgca
     3181 ccgggcacac gatgttttcg gcgttgccgc gtccctgcag catgatggcc tggcgatggc
     3241 ggcagacgtt ggagagcagc tccacgccgc tcgcgtttcg cacgagcacc ttggcgtggt
     3301 ccatccatgc gagggtgtgc cagtcgcccg cgttgggcac catgagctcg tggccgacgt
     3361 aggtcggccc ggcctggaag acgagccgct tctccgcttc gaagacgtgc gggtcgaaat
     3421 accacgagac cggcagctga cagcttgcgg gctttagttg tttaagcttg gcaacatccg
     3481 ccatgcgttg taaagctcct agaaaaacag tattttagca aacgatgagc catccttcag
     3541 ccgagcctcg acagctcgac tttgaaaagg cgctcgccga actggaaaaa atcgtcgccc
     3601 gcatggaaag cggcgagctc tcgctcgagc aggcgctcgc tacgcataag cgcggcatcg
     3661 agctcgcgcg gttctgccag cagaagctgg aggcggcgca gcagcaagtc aaggtcctcg
     3721 aaggagaagc cctcaagccg ctcgcggcgg gcgagcccga tgaagattct tgacagcgag
     3781 ttccaggcct ggatgggcgg cgtccaggcg cgcatggaga cggtgctcgc gcgcgttctt
     3841 ccgtcgccgg gcgtggcgcc ggtgcggctg caccaggcga tgcgctatgc ggcgctcgga
     3901 ggcggcaagc gcgtgcgccc gttgctcgct tttgccgcgg gcgaggtgtc gcaggcgccg
     3961 cccgagcggc tcgagatcgc ggcggcggcg gtggagctcg ttcatgccta ttctcttgtg
     4021 cacgacgatc tgccttgcat ggacgacgac gtcctgcggc gcgggaagcc cacggtgcac
     4081 gtcgagtacg acgaagccac cgcattgctc gtgggtgatg cgctgcaatc gctcgccttc
     4141 cacttgctcg cggagcacgc gatcgcggat gatccgcgcc ggcagctcga gatggtgaag
     4201 accctggcac gcgccgcggg ctcgcgcggc atggcgggcg gacagcagac cgacctcgag
     4261 gcgaccggca ggacgctcga cctgccggag ctggagttca tgcatatcca caagaccggc
     4321 gcgctgatcc gggccgccgt gcggctcggc gcggcatgcg gaaaggagct cgacgcggaa
     4381 agccagaagc tcgacaggta cgccaaggcg atcgggctcg ccttccaggt ggtggatgac
     4441 gtgctcgact acgacgcgag cacggcgacg ctcggcaaga cggcgggcaa ggattcgaag
     4501 cacggcaagc cgacgtacgt atcggccatg ggcatcgcgc gcgcgcgcag cctggcggag
     4561 gagctgcgcg cggaggcgca cgcggcgctc gccggcatcg gcgcccgcgc gcggcgcctg
     4621 ggcgagcttg ccgacttcat cgtcctgaga aagttctcgc gatggccgcg atcagtcacg
     4681 agctgccggc tgacggcggc gagccggcca gctacgcgct gctgcgcacg atcgacgacc
     4741 cggcgcagct gcgcgcgctc gatcgcaggc agcttggtca gctcgccgag gagctgcgcg
     4801 cgttcgtgct cgaatcggtc tccaagacag gcggccactt gtcgtcgaat ctcggcaccg
     4861 tggagctcac gatcgcgctg cactacgtgt tcgacacgcc cgaggaccgc atcgtctggg
     4921 acgtcggcca ccagacctac ccgcacaaga tcctcactgg ccggcgcgac cggatgaaga
     4981 cgttgcgcca gtacggcggg ctctccggct tctgcctgcg cagcgaatcg gagtacgacg
     5041 tcgtcgccgc cggccacgcc tcgacctcga tctccgccgc cctcggcctc gcggtggcgc
     5101 gcgatctc
//
LOCUS       DASUNT010000362         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_402266, whole genome shotgun sequence.
ACCESSION   DASUNT010000362 DASUNT010000000
VERSION     DASUNT010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402266"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..578)
                     /locus_tag="VFX94_07340"
     CDS             complement(111..578)
                     /locus_tag="VFX94_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX5768031.1"
                     /translation="MKARGFTLIELVTAVAIVAVLASAVLPLNELVVQRAKEQDLRRA
                     LRDIRTAIDAYKQASDEGRIPKRVGESGYPKRLEDLVQGVEDEKSPKRERMYFLRRLP
                     RDPFAANEVSSAESWGKRSYASPPDDPREGDDVFDVYTLALGKGINGRPYREW"
     gene            complement(575..2425)
                     /locus_tag="VFX94_07345"
     CDS             complement(575..2425)
                     /locus_tag="VFX94_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HEX5768032.1"
                     /translation="MGARFFVLILLAAGCASMEDPVVRQAQELFSTGRGEQALALLEK
                     DPKHRGEYMRTRDLLAAQWLAQAEVLRINAQPEAAAELLRRVQKYDPASPRARAGLEQ
                     IELDARHRPIVANAERLVRDESYLEAQDLLRPVLIENPQSRDARRLQRLIEERTAKPA
                     IVTPRLKTSTTQPISLEMRDVALRAVFDMIARATGVSFVFDKDVRADQRTTIVVRNAS
                     PEELIRLVLATNQLEQKIVNETTVLVFPNTPQKLREYQELVVKSFYVANADVRQTANM
                     IRTMVKTRDIFVDEKVGLLVIKDTPNAVRLAERLIAAQDLAEPEVMLQVEVLEMSRNR
                     LLELGIKYPDSVAWSLVGAAGVPGVVSLPEWLNRSSDIVQLRFTDPLFLVSFLQQDGS
                     SNVLANPRIRVKNKEKARIHIGDRVPVITTTAAATGGFVSESVSYLDVGLKLEVEPII
                     YLEDEVGIRVGLEVSNIAREIRTVGSNTLTYQIGTRNATTMLRLRDGETQILAGLIND
                     QDRSTASRVPGIGDLPVLGRLFSHTRDEMLKTEIVLLITPRLVRTLARPDARSLEFAA
                     GTEASAGSPAGAVPLVLPPQPQPQPPAPAKPPAPPAGAEQVPFGGVKPPQ"
BASE COUNT          372 a          821 c          883 g          444 t
ORIGIN      
        1 gatggtgagc agcgtggcga tgatcgccag caccacgagg agctcgatga gggtgaaacc
       61 ccccgctcgc gtcgctcgcg acccccttgt cagggggtca tagaacgttc tcaccattcg
      121 cggtacgggc ggccgttgat acctttgccg agcgcgagcg tatagacgtc gaacacgtcg
      181 tcgccctcgc gcggatcgtc gggcgggctc gcgtaggagc gtttgcccca ggattcggcg
      241 gaggaaacct cgttcgctgc gaacggatcc cggggaagcc ggcgcagaaa gtacatgcgc
      301 tcgcgcttcg gactcttctc gtcctccact ccctgtacga ggtcctcgag gcgcttcggg
      361 tagcccgatt ccccgacgcg cttcggaatg cggccctcgt cggaggcttg cttgtaggcg
      421 tcgatcgcgg tgcggatatc gcgcagcgcg cgcctcaggt cctgctcctt tgcgcgctgc
      481 acgacgagct cgttgagcgg caagaccgcc gaggctagga cggccacgat cgccaccgcg
      541 gtgacgagct cgatcagcgt gaatccgcgc gccttcattg cggcggcttg actcctccga
      601 acggtacctg ctcggcgcca gcgggcgggg cgggcggttt ggcgggcgcc ggcggctggg
      661 gctgcggttg cggcggcagg accaggggca cggcgccggc cggcgagccg gcggacgcct
      721 cggtgccggc ggcgaactcc agcgagcgcg cgtcgggccg cgccagcgtc cgcaccaagc
      781 gcggcgtgat aagaagcacg atctcggtct tcaacatttc gtcccgcgtg tgcgagaaga
      841 gccgtccgag caccggaaga tcaccgatgc ccggaacgcg gctcgccgtg ctgcggtcct
      901 ggtcgttgat cagtccggca aggatctggg tctcgccgtc gcgtagccgc agcatggtgg
      961 tcgcattgcg cgtgccgatc tggtaggtga gcgtgttgct gcccacagtg cgaatctcgc
     1021 gcgcgatgtt gctgacctcc agccccaccc ggatgccgac ctcgtcttcg aggtagatga
     1081 tgggctcgac ctcgagcttc aagccgacgt ccagatagga caccgactcg gagacgaagc
     1141 cgccggtcgc ggcggcggta gtggtgatta ccggcacgcg gtcgccgatg tggatgcgcg
     1201 ccttctcctt gttcttgacc cggatgcgcg ggttggcgag cacgttgctc gagccgtcct
     1261 gctgcaggaa gctgacgagg aaaagcgggt cggtgaaccg caactgcacg atgtcggagc
     1321 tgcggttgag ccactcgggc aggctcacca cccccgggac gccggcggcg cccaccaggc
     1381 tccaagccac cgagtcgggg tacttgatgc cgagctcgag caggcggttc ctgctcatct
     1441 cgaggacctc gacctggagc atcacttcgg gctcggccag gtcctgcgcc gcgatcagcc
     1501 gctcggcgag gcgcaccgcg ttcggcgtgt ccttgatgac gagcagaccc accttctcgt
     1561 cgacgaagat gtcgcgcgtc ttgaccatgg tgcggatcat gttcgcggtc tggcgcacgt
     1621 cggcgttcgc gacgtagaag ctcttcacca cgagctcctg gtactcgcgc agcttctgcg
     1681 gcgtgttggg gaacacgagc accgtggtct cgttgacgat cttctgctcg agctggttcg
     1741 tggcgagcac caggcggatg agctcctcgg gcgaggcgtt gcgcaccacg atggtggtgc
     1801 gctggtcggc gcgcacgtcc ttgtcgaaca cgaagctcac accggtcgcg cgcgcgatca
     1861 tgtcgaatac cgcgcgcagc gcgacgtcgc gcatttcgag cgagatcggc tgggtggtcg
     1921 aggtcttgag acgcggggtg acgatggcgg gcttcgccgt gcgctcctcg atcaggcgct
     1981 gcagccgcct cgcgtcgcgg ctctgcgggt tctcgatcag cacgggacga agcaggtcct
     2041 gcgcctcgag gtagctctcg tcccgcacga gcctctcggc attggcgacg atcgggcggt
     2101 ggcgcgcgtc gagctcgatc tgctcgagcc cggcgcgcgc gcgcgggctg gcgggatcgt
     2161 atttctggac gcggcgcagg agctcggccg cggcctcggg ctgagcgttg atgcgcagca
     2221 cctccgcctg ggcgagccat tgcgcagcga gcaggtcgcg ggtgcgcatg tattcgccgc
     2281 gatgcttcgg gtccttctcg agaagcgcga gcgcctgctc gccgcggccc gtagagaaga
     2341 gctcctgcgc ctgcctgacc accgggtcct ccatcgaggc gcagcctgca gccagaagta
     2401 tcaatacgaa gaagcgcgct cccattacgt gccctttcgc aagtagatcg tgaggcggac
     2461 ctgcgcctcg agctgcgcgt ctgcggcttt tttccgctcg aagcgcagcg cgtcgagcga
//
LOCUS       DASUNT010000363         2213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_927305, whole genome shotgun sequence.
ACCESSION   DASUNT010000363 DASUNT010000000
VERSION     DASUNT010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927305"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFX94_07350"
     CDS             <1..274
                     /locus_tag="VFX94_07350"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344137.1"
                     /note="converts protoheme IX and farnesyl diphosphate to
                     heme O; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HEX5768033.1"
                     /translation="MLPVTHGKKYTRLQVLLYTLILFAVSLLPYVIRMAGVVYLAAAV
                     TLGALFVSYALKIYLAYTDAVAQKTFRYSIAYLAFLFAALLVDHYL"
     gene            231..815
                     /locus_tag="VFX94_07355"
     CDS             231..815
                     /locus_tag="VFX94_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX5768034.1"
                     /translation="MRSFSRLFWSIITCSLAAAAGCDSSPQFKSTDITGAPYGRSLEL
                     TDHTGKPRRLEDWRGKAVVLFFGFTRCPDVCPTTLADIAHALKELGPDAARVQVLMVS
                     VDPERDTPDALAKYVTAFDPAFVGLRGELEATRKVAQEFKIYFEKRKQGDTYTVDHSA
                     QSYVIDPQGRLRLLVRHDRIVQDLAHDLRALLRG"
     gene            complement(864..1118)
                     /locus_tag="VFX94_07360"
     CDS             complement(864..1118)
                     /locus_tag="VFX94_07360"
                     /inference="COORDINATES: protein motif:HMM:NF020434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HEX5768035.1"
                     /translation="MRIVLWPLPVAYACQGCPEFGQRARDFASALDHDGVAEMVWLGA
                     AKEINPTQRYPVFAVDGCSKGCALRWLAEHGITAERSYIV"
     gene            complement(1119..1241)
                     /locus_tag="VFX94_07365"
     CDS             complement(1119..1241)
                     /locus_tag="VFX94_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768036.1"
                     /translation="MGSTVSRQQISVVTLSTIAFTVCFAVWMMFAVIGIPIKKM"
     gene            complement(1326..1769)
                     /locus_tag="VFX94_07370"
     CDS             complement(1326..1769)
                     /locus_tag="VFX94_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5768037.1"
                     /translation="MYKHILIPTDGSEVAEKAIAQGIEFAREAKARVTLFTAVPEYEP
                     PSEAQVFARRVVSIAEHARECEQTAKQILERPAQLARAAGLQFDTDYEQSNQPWQAIV
                     DAAKRHGCDAIFMASHGRKGLSRLVHGSQTVDVLTHSDIPTLVVR"
     gene            complement(1791..>2213)
                     /locus_tag="VFX94_07375"
     CDS             complement(1791..>2213)
                     /locus_tag="VFX94_07375"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5768038.1"
                     /translation="RLHHPKENDHLFSRLVARDPGARLLVEELQAEHEEGARLIRELE
                     RALLFFEEGWPAGAREFQQTVDAYADFHWKHMRKEEQQLLPLAERALSAEDWKAINQA
                     FAENRDPVAGMKERDYEQLFSRIANLAPAPVGLGASWR"
BASE COUNT          355 a          762 c          694 g          402 t
ORIGIN      
        1 gatgctgccg gtcacgcacg gcaagaaata cacccggctg caggtccttc tctatacgct
       61 gattctcttc gccgtcagcc tgcttcccta cgtgatccgc atggccgggg tggtgtacct
      121 ggcggcggcc gtcacgctcg gcgcgctgtt cgtgtcgtat gcattgaaga tctacctcgc
      181 ctacaccgat gccgtggcgc aaaagacctt ccgctattcg atcgcttacc ttgcgttcct
      241 tttcgcggct cttctggtcg atcattacct gtagcctggc ggctgcagcc ggctgcgatt
      301 cgagcccgca gttcaagagc accgacatca ccggggcgcc gtacggccgc tcgctcgagc
      361 tcaccgacca cacgggcaag ccgcggcgcc tcgaggactg gcgcggcaag gcggtggtgc
      421 tgttcttcgg ctttacccgc tgccccgacg tctgtccgac cacgctcgcg gatattgcgc
      481 atgcgctaaa ggagctcggc cccgacgccg ctcgcgtcca ggtgctcatg gtgagcgtcg
      541 atcccgagcg cgacacgccc gatgcgctcg cgaagtacgt caccgccttc gatccggcat
      601 tcgtcggcct gcgcggcgag ctcgaagcga cccgcaaggt cgcgcaggag ttcaagatct
      661 acttcgagaa gcgcaagcag ggcgacacct acaccgtcga ccacagcgcg cagagctacg
      721 tcatcgatcc gcagggcagg ctgcggctcc tggtgcgcca cgaccgcatc gtccaggacc
      781 tcgcgcacga cctgcgggcg ctgctgcgcg gctgagaaaa gggggcagga cccttttcca
      841 taaaagggtc ctgccccctt ttttcagacg atgtagctgc gctccgcggt gatgccgtgc
      901 tcggcaagcc accgcagcgc gcaccctttg ctgcagccgt ctaccgcgaa caccgggtaa
      961 cgctgcgtcg ggtttatctc cttggctgcg ccgagccaca ccatctcggc tacgccgtcg
     1021 tggtcgagcg cgctcgcgaa gtcgcgggcg cgctgcccga actccggaca gccctggcag
     1081 gcgtaggcga ccggcagcgg ccacaggacg atacgcatct acatcttctt gatggggatg
     1141 ccgatcaccg cgaacatcat ccacaccgcg aagcacaccg tgaacgcgat cgtgctcagc
     1201 gtgacgacgg aaatttgctg ccggctcact gtagagccca ccgcgcgact ctccttctcg
     1261 aattcagact ttagagacgc gcacccaagt tcgcttgatc cacatcaagc gaaccgcgga
     1321 accggctaac gcacgaccag cgtgggtatg tcggaatgtg tcaggacgtc gacggtctgg
     1381 ctgccgtgca ccaggcgcga caggcccttg cgcccgtgcg aagccatgaa gatcgcgtcg
     1441 cagccgtgcc gcttggccgc gtccacgatc gcctgccacg gctggttgct ctgctcgtag
     1501 tcggtgtcga attggagtcc ggccgcgcgc gcgagctgcg cgggacgctc gaggatctgc
     1561 ttcgcggttt gctcgcactc gcgcgcatgc tcggcgatcg agaccacgcg gcgcgcgaac
     1621 acctgcgcct cgctcggcgg ctcgtattcg ggcaccgcgg tgaaaagcgt cacgcgcgcc
     1681 ttcgcttcac gcgcgaactc gatgccctgc gcgatcgcct tctcggcgac ttcagagccg
     1741 tccgtgggga tgaggatgtg cttgtacata aggccactcc tggagttggg tcatcgccag
     1801 ctcgcgccga ggccgaccgg cgccggggca aggttggcga tgcgggaaaa gagctgctcg
     1861 tagtcgcgct ccttcatccc ggccaccggg tcgcggtttt ccgcgaacgc ctggttgatc
     1921 gccttccagt cctcggcgct cagcgcgcgc tccgcgagcg gcagtagctg ctgctcctct
     1981 ttgcgcatgt gcttccagtg gaaatcggcg taggcatcga ccgtctgctg gaactcgcgt
     2041 gcaccggcgg gccagccctc ctcgaagaag agcagcgcgc gctcgagctc gcggatgagg
     2101 cgcgcgcctt cctcgtgctc ggcctgcagc tcctcgacga gaagccgcgc gcccgggtcg
     2161 cgcgccacca gccgcgagaa gaggtgatcg ttctccttcg gatggtgcaa ccg
//
LOCUS       DASUNT010000364         2729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_139894, whole genome shotgun sequence.
ACCESSION   DASUNT010000364 DASUNT010000000
VERSION     DASUNT010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139894"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="VFX94_07380"
     CDS             <1..1205
                     /locus_tag="VFX94_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768039.1"
                     /translation="EGWETKIRALLKGNDPGKVRHVNAVLDAMDQLASVRRKYGDVSL
                     ADRIEARTSKLRRTMGLANEVADASNRMKALGELIGGVPMGGAIAGGLAGGLPGAAIG
                     AALPGAVRGLVMGDLIEAFQRLSGASERAAQRGVDDWVRSSRVRGQGLKVPRVPPLTE
                     AGRQLRDVAARRGVTQSMALFMGDDESPHAAFVRWRDALMDQPAFFRGLGDDYAALKD
                     EAPEVFMAIGGRADLARRFLIERMPPNTGVSVANPEGYLPSRDSIEDWALYVNAVRFP
                     MRVLRNIGGAFMQEIETLRTVHPRVHEQAQQQVLRAMAKANTAGEALDDTFYARAQLL
                     FPDIDGLGSPIFSRDFGRFVAEYNAEQKELQMQKAGGGRPGKPRQPPLSPTQQTIQSG
                     ATYGSGLA"
     gene            complement(1191..1715)
                     /locus_tag="VFX94_07385"
     CDS             complement(1191..1715)
                     /locus_tag="VFX94_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768040.1"
                     /translation="MRPERIVIVGGPRCGKSQLSKALRAHGFPTFCGDPIDKVKEPEP
                     RVTYLAEGMGMDSASSRYIVDRWLPMPGPWVLEGHIMARVLRKWLDDDARGDEGTGSH
                     TWPCDRIVVFEEQHPDADLSARQVAHHKGVIGTTWRGIAEYFEGMTTTIRWSDYRFAD
                     VTPEELASQVLSQP"
     gene            complement(1712..1885)
                     /locus_tag="VFX94_07390"
     CDS             complement(1712..1885)
                     /locus_tag="VFX94_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768041.1"
                     /translation="MKLGLYEVLRARLKGKARRPTGYVQLELLTHPDDPNLVVGARLW
                     CTVKPWGKHPVKV"
     gene            complement(1921..2556)
                     /locus_tag="VFX94_07395"
     CDS             complement(1921..2556)
                     /locus_tag="VFX94_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768042.1"
                     /translation="MPTSQGPAKPKLKDDLGEPEILAITPVAAPNWALSTQPHSAGQA
                     FAIAKRLHQSGLWTQRFKTEAQVAAIILRGRELGIGMTTALEAFYIVRGRPFASAQLI
                     SGLAEQDKNHDYAMLKHLDTKRAVVEIKHKRQPKPVDWEYTIDEAEKLGLLAPSKETG
                     GLSQWVLRPKTMLAKTVRVIGERYMFPAATLGLHALEMEPEAQHLVKADDS"
BASE COUNT          411 a          898 c          951 g          469 t
ORIGIN      
        1 tcgaaggctg ggagacgaag atccgcgcgc tgctgaaagg caacgacccc ggcaaggtgc
       61 ggcacgtgaa cgcggtgctc gatgcgatgg accagctcgc cagcgtcagg cggaagtatg
      121 gcgacgtgtc tttggccgac cgcatcgagg cgcgcacgag caagctccgg cgcaccatgg
      181 ggctcgccaa cgaggtggcg gacgccagca accgcatgaa ggcgctcggc gaactcatcg
      241 gcggcgtgcc catgggcgga gccatcgccg gcggtctcgc gggcgggctg ccgggcgcag
      301 ccatcggcgc ggcgctgcct ggcgctgtgc gcgggctggt gatgggcgac ctgatcgagg
      361 ccttccagcg cctgagcgga gcgagcgagc gcgcggcgca gcggggcgtt gacgactggg
      421 tgcgctccag ccgggtgcgc gggcaaggcc tgaaggtgcc gagggtgccg ccgctcaccg
      481 aggcggggcg ccagctgcgc gacgtggctg cccgacgcgg cgtcacgcag tcgatggcgc
      541 tgttcatggg cgacgacgag agcccccacg ctgctttcgt gcgctggcgc gacgcgctga
      601 tggaccagcc cgctttcttc agggggctcg gcgacgatta cgccgcgttg aaggatgagg
      661 cgccagaggt gttcatggcg atcggcggcc gcgcggacct ggcgcggcgc ttcctgatcg
      721 agcgcatgcc gcccaacacg ggcgtttcgg tggccaaccc cgagggatac ctcccatcgc
      781 gcgactcgat cgaggattgg gcgctctacg tgaacgcggt tcgcttcccg atgcgggtgc
      841 ttcgcaacat cggcggcgcg ttcatgcagg agatcgagac gctgcgcacg gtgcacccgc
      901 gcgtgcacga gcaggcgcaa cagcaggtgc tacgggcgat ggcgaaggcg aacaccgccg
      961 gcgaggcgct cgatgatacc ttctacgcgc gcgcgcaact gctgttccct gacatcgacg
     1021 ggctgggttc tcccatcttc tcgcgcgact ttggccggtt cgtggccgag tacaacgccg
     1081 agcagaagga gcttcagatg cagaaggcag gcggcgggcg ccccggtaag ccgaggcagc
     1141 cgccgctttc tccaacgcag cagacgatcc agagcggcgc tacgtacggc tcagggctgg
     1201 cttagaacct gagacgcgag ctcctctgga gtgacgtcag cgaaccgata atcgctccac
     1261 cggatcgtag tggtcatccc ttcgaagtat tcagcgatcc cgcgccacgt cgttccgatc
     1321 actcccttgt ggtgcgcgac ctggcgcgcg ctgaggtcag cgtccgggtg ctgttcctcg
     1381 aacaccacga tccgatcgca gggccacgta tggctgcctg taccctcgtc tccacgcgcg
     1441 tcgtcgtcga gccacttgcg cagcacgcgc gccatgatgt ggccttcgag cacccacggc
     1501 cccggcatcg gcagccagcg atccacgatg tagcgcgagc tcgccgagtc catgcccatg
     1561 ccttcggcga ggtaggtcac ccggggctct ggctccttca ccttgtcgat cgggtccccg
     1621 cagaacgtgg ggaacccatg cgcgcgcagc gccttcgaga gctgcgactt cccgcagcgc
     1681 ggcccgccaa cgatcacgat tcgctctggt ctcatacctt caccgggtgc tttccccagg
     1741 gcttgaccgt gcaccacagc cgagccccga caacgagatt aggatcgtcg gggtgcgtca
     1801 gcagctcgag ctgaacgtac ccagtggggc gccgcgcctt acccttcagg cgcgcgcgca
     1861 ggacttcgta aaggccgagc ttcacatcct cgggtgacag ctcgtaccgc ttcccctcgc
     1921 tcacgagtcg tccgccttca ccaggtgttg cgcctcgggc tccatctcga gcgcgtggag
     1981 cccgagcgtt gcggcgggga acatgtagcg ctcaccgatg acgcgcaccg ttttggcgag
     2041 catggtcttc gggcgcagca cccactggct tagcccgccc gtctctttgc tcggtgcgag
     2101 aagcccgagc ttttcggcct cgtcgatcgt gtactcccaa tccacgggtt ttggctggcg
     2161 cttgtgcttg atctcgacca cggcccgctt cgtgtcgagg tgcttcagca tcgcataatc
     2221 gtgattcttg tcctgctcgg ccaggccact gatgagctgc gccgacgcga atgggcgccc
     2281 gcgcacgatg tagaacgctt cgagcgctgt cgtcatgccg atcccgagct cgcggccgcg
     2341 caggatgatc gcggcgactt gagcctccgt cttgaagcgc tgcgtccata ggccgctctg
     2401 atgcagtcgc ttcgcgatgg cgaaggcctg gccagcgctg tgcggctggg tcgagagcgc
     2461 ccagttgggc gccgctaccg gggtgatggc gaggatctca ggctcgccga gatcgtcttt
     2521 cagctttggt tttgcgggcc cttggcttgt cggcacgact tcgacgcgcc cgctcggctg
     2581 gggggaggag ctcgacggtg cagcggctgg ctgcgttgca gctggttggg gtgacgcaga
     2641 tgtgccagcg gcggaatggg caggctgggt accagcgggc tccgacttcg tggcagtcgg
     2701 ggcgctcggg cccgagccgg cggggggcg
//
LOCUS       DASUNT010000365         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_840735, whole genome shotgun sequence.
ACCESSION   DASUNT010000365 DASUNT010000000
VERSION     DASUNT010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840735"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..180
                     /locus_tag="VFX94_07400"
                     /pseudo
     CDS             1..180
                     /locus_tag="VFX94_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(177..1736)
                     /locus_tag="VFX94_07405"
                     /pseudo
     CDS             complement(177..1736)
                     /locus_tag="VFX94_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
BASE COUNT          328 a          685 c          684 g          390 t
ORIGIN      
        1 gtggccgacg acctgaccgg catctacgac gccatcaagg aaaacgcgct gctggccaag
       61 tacgccgggg gcctcggcaa cgactggacg ccggtccggg cgctcggctc gcacatcaag
      121 ggcaccaacg gcaagtcgca gggcgtagtg ccgttcctca aggtggtgaa cgacaccgcc
      181 gggatcttct tgaagagctt cagctcgccg cgcgccaccc tgtcctcgca gcgcaggtag
      241 gcttcctcga actccttgcc gtacttgtcg tgcaggtcgc gcgcgtcgga ggggctgaac
      301 agcgtccact ccgcgccctg catcacccgc ttcatgaaca gatcggggat ccagttggcg
      361 gtgttcatgt cgtgcgtgcg ccgccggtcg tcgccggtgt tcttgcgcag ctcgaggaac
      421 tcctcgatgt cgaggtgcca cacctcgagg taggagcaca ccgcaccctt gcgcttgccg
      481 ccctggttga ccgccacggc ggtgtcgttc accaccttca ggaacggcac cacgccctgc
      541 gacttgccgt tggtgccctt gatatgcgag ccgagcgcgc gcaccggcgt ccagtcgttg
      601 cccaggccgc cggcgtactt ggcgagcagc gcgttttcct tgatcgcgtc gtggatgccg
      661 gtgaggtcgt ccgacacggt ggtgaggtag cagctcgaca gctgcgagcg cagcgtgccg
      721 gagttgaaca gcgtcggcgt cgagctcatg aagtcgaagc tcgagagcgc gtcgtagaac
      781 tcgatggcgc gcgcttcgcg atgcttctcc tgcagggcca ggcccatcgc cacgcgcatg
      841 aagaacgcct gcggcagctc gatgcgctgg ccgtcgctgt gcaggaagta gcggtcgtac
      901 agggtctgca ggccgaggta gccgaattgc agatcccgcc ttgccttcag cgcttttccc
      961 aaccgatgca aatcgaactt ggccagctcg gggtccagca gctcggcctc gacgccgcgc
     1021 gcgatgaact gcgggaagta ctcggcgtag cgcgtctcca tctcggcctg gctcgcctcc
     1081 tcgcccagca cctccaggcg gatggtgtcg agcagcagcc gcgcggtgac gaagctgtag
     1141 gccgggtcct tctcgatcag cgcccgcgcc gcgagcaccg aggatttgcg cacctcctgc
     1201 atcggcacgc cgtcgtacag gtcgcgcagg gtggcttcga ggatcgcctg cggcgcggcg
     1261 tcggccagcc cttcgcacgc cgactcgacc aggcgctgca gccgcgcgcg gtcgagcggc
     1321 ttgcgcgcgc cgttctcgac cacgtggatc tcatccttct tcgagctctt ctcctgcgcg
     1381 cgcgccttgg cgcgctcctc gcggtacagc acgtaggcgc gggcgacgtc gtgctcgccc
     1441 gagcgcatca gcgcgagctc gacctggtcc tggatgtctt caatatggaa ggtgccgccc
     1501 tgcggctggc ggcgcagcag cgcgttgacc acgttgtcgg tcaggtcggc gaccatggtg
     1561 cggatgcggg cggaagcggc gccctgggcg ccgttcaccg ccaggaacgc cttggtcatc
     1621 gccaccgcga tcttgccggg ctcgaagccg accaccgcgc cgttgcggcg gatgatcttg
     1681 tagtccgcgt aagggctgcg cgccgcgctg ccggtctcag gaagggctac gcccagggtc
     1741 gaggcggcgg cggcgttttc ctgagtgatc tgcatatctc aacctctccc gtgtggtgcg
     1801 ttttgccggg accgtctgcc gcgttgttgt tgcggttctt gacgacctgc cgttcgctta
     1861 caacatctag ttgcgtcttc aagcttcaac acaacatgtg gtgcagcgta cccgctaatt
     1921 tttttctttg caagcgcgag ctactaaata tagtgtaaag tgcgacatta aaacctagat
     1981 gtaggacgaa tgcggcggcg cacaacaaca tctgggcgtg gcgcgcgcgc cacaactaca
     2041 tacagtgtga atttatgagc gcgcggcgag cgcggcgagc aggcgcg
//
LOCUS       DASUNT010000366         2590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_490360, whole genome shotgun sequence.
ACCESSION   DASUNT010000366 DASUNT010000000
VERSION     DASUNT010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490360"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..591)
                     /gene="metW"
                     /locus_tag="VFX94_07410"
     CDS             complement(13..591)
                     /gene="metW"
                     /locus_tag="VFX94_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX5768043.1"
                     /translation="MRPDLQAIVHWVPQRARVLDLGCGDGSLLRSLWQERQAPGYGVE
                     IEDQAVLACVENDVNVLQVDLESGLSLFADGSFDCVILSETLQTIHRTEFLLREMLRV
                     GREVIVSFPNFGHWRARLQIALGRMPVSETLPHQWYETPNVHHCTITDFEDLCRRIGI
                     RIRERLVLNDGKPVTLLPNFLGSVAVYRCTQS"
     gene            complement(588..1727)
                     /locus_tag="VFX94_07415"
     CDS             complement(588..1727)
                     /locus_tag="VFX94_07415"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX5768044.1"
                     /translation="MNAPHHDSGSIGAAKAQKAHFNEPLRLRGGCELPAYEIAYETYG
                     RLNEARSNAVLVCHALNASHHVAGYYEDDPQNIGWWDNLVGPGKPLDTNRFFVVGSNY
                     IGSCFGSTGPASINPATGKPWGADFPVVTVEDWVQAQARLAERLGIERFAAVIGGSLG
                     AMQALQWTLSYPERVRHSIVIAAAARLTPQNIAFNEVARQAIMTDPDFHGGHYYDKGV
                     VPTRGLRIARMIGHITYLSGEAMSEKFGRLLRRGAPGFDFDIDFEIESYLRYQGDKFS
                     SYFDANTYLRITKALDYFDPAADYGGDLAKALAQARAAFLVVSFKSDWRFPPARSRDI
                     VRALLDTRRIESYLELDAPGGHDAFLLEDPRYHSALRHYFANIDL"
     gene            complement(1797..>2590)
                     /locus_tag="VFX94_07420"
     CDS             complement(1797..>2590)
                     /locus_tag="VFX94_07420"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX5768045.1"
                     /translation="AYDDMIQGASGLPWLLQKQGSPEPRYAPMIVADRSVGQQVASAV
                     SAALYYREKTGKGQRVDVPMWEHLLQIVLGDHLGGYTFDPQHGEPGYARILTPDRRPY
                     QTADGYVCALIYTNKQWKSFLGIIGKPQMLERPEYATQEARSQNYRESYAMVAEEMKR
                     RTTAFWLEALERGDIPVQRMNTLDDIVADPHLNAIGYLKMVEHPSEGRIRSLAVPSEW
                     SQSAPEYRRHAPRLGEHTREILGEAGFSGESIDRLISEGAAKALD"
BASE COUNT          404 a          818 c          921 g          447 t
ORIGIN      
        1 tcgtaggcgc tttcagcttt gagtgcagcg gtacacggcc acgctgccga ggaagttcgg
       61 cagcagggtg accggcttgc cgtcgttaag cacgaggcgc tcgcggatgc ggatcccgat
      121 gcgccggcac aggtcctcga aatcggtgat cgtgcagtgg tggacgttcg gggtttcgta
      181 ccactgatgc ggaagcgtct cggagaccgg catgcggccg agcgcgatct gcaggcgcgc
      241 gcgccagtgg ccgaagttgg gaaagctcac gatgacctcg cggcccacgc ggagcatctc
      301 gcgcagcagg aactcggtgc ggtggatggt ctggagcgtc tcggaaagga tcacgcagtc
      361 gaacgagccg tcggcgaaga gcgaaagccc gctctccagg tcgacctgca gcacgttgac
      421 gtcgttctcg acgcaggcaa gcacggcctg gtcctcgatt tcgacgccgt agccgggcgc
      481 ctggcgctcc tgccagagcg agcgcaggag cgagccgtcg ccgcagccca ggtcgagcac
      541 gcgcgctcgc tgcggcaccc aatgcacgat ggcctgaagg tcgggtctca cagatcgatg
      601 ttcgcgaagt agtggcgaag cgcggagtga tagcgcgggt cttcgagcag gaacgcgtcg
      661 tgcccgcccg gcgcgtcgag ctcgaggtaa gactcgatgc gccgggtgtc tagcagcgcg
      721 cgcacgatgt cgcgcgagcg cgccggcggg aagcgccagt cggacttgaa cgacaccacg
      781 aggaacgctg cccttgcttg ggcgagcgcc ttggcaaggt cgccgccgta gtcggccgcc
      841 gggtcgaagt agtcgagcgc cttggtgatg cgcaggtacg tgttcgcgtc gaagtagctc
      901 gagaacttgt caccctggta gcgcaggtag gactcgatct cgaagtcgat gtcgaagtcg
      961 aagcccgggg cgccgcggcg cagcagccgg ccgaactttt cgctcatcgc ctcgccggag
     1021 aggtacgtga tgtggccgat catgcgcgcg atgcgcagtc cgcgcgtggg cacgacgccc
     1081 ttgtcgtagt agtggccgcc gtggaagtcg gggtcggtca tgatcgcctg gcgcgccacc
     1141 tcgttgaacg cgatgttctg aggcgtgagc cgggcggccg cggcgatcac gatcgagtgg
     1201 cggacgcgct ccgggtagga gagcgtccac tgcagggcct gcatggcgcc gagcgagcca
     1261 ccgatgacgg cggcgaagcg ctcgatgccc aggcgctccg cgagccgcgc ctgcgcctgc
     1321 acccagtcct ccaccgtgac caccgggaag tcggcgcccc acggcttgcc ggtcgcgggg
     1381 ttgatcgagg ccggtccggt ggagccgaag cagctgccga tgtagttgga gcccacgacg
     1441 aagaaccggt tggtgtcgag cggcttgccc gggccgacga ggttgtccca ccagccgatg
     1501 ttctgcggat cgtcctcgta atatcccgca acatggtgcg acgcattgag cgcgtggcac
     1561 acgaggaccg cgttcgagcg cgcctcgttg aggcgcccat aggtctcgta cgcgatttcg
     1621 taggcgggca gctcgcagcc gccgcgcagc cgcagcggct cgttgaaatg cgctttctgc
     1681 gccttggcgg cgccgatcga gccggaatcg tgatgaggtg cgttcatctc tttagcggta
     1741 tttataaggc gcccgcaagc tgatcatcaa atcggcgcgc cgcggattat agcgttctag
     1801 tccagggctt tcgccgcccc ctcggagatg agccggtcga tgctttcgcc cgagaagccc
     1861 gcttcgccga ggatctcgcg ggtgtgctcg cccaggcgcg gcgcgtggcg ccggtactcc
     1921 ggcgccgatt gcgaccactc cgacggtacg gcgagggagc ggatgcggcc ttcgctcggg
     1981 tgctccacca tcttcaggta accgatcgcg ttgaggtgcg gatcggccac gatgtcgtca
     2041 agcgtgttca tgcgctgcac ggggatgtcg ccgcgctcga gcgcctcgag ccagaacgcg
     2101 gtcgtgcgcc gcttcatttc ctccgccacc atggcatagg actcgcgata gttctggctg
     2161 cgggcctcct gcgtcgcata ttcgggccgc tccagcatct gcggcttgcc gatgatcccg
     2221 aggaagctct tccattgctt gttggtgtag atgagcgcgc acacgtagcc gtcggcggtc
     2281 tggtacgggc gccggtcggg cgtgaggatc cgcgcataac cgggctcgcc gtgctgcgga
     2341 tcgaacgtgt agcccccgag gtggtcgccg agcacgattt gcaggaggtg ctcccacatg
     2401 gggacgtcga cgcgctgtcc cttgccggtc ttctcgcggt agtagagggc ggcgctcacc
     2461 gcgctcgcca cctgctggcc gacggagcgg tccgcgacga tcatcggcgc gtagcgcggc
     2521 tcgggcgagc cctgcttctg caggagccat ggcagccccg aagcgccctg gatcatgtcg
     2581 tcgtacgcgg
//
LOCUS       DASUNT010000367         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_927909, whole genome shotgun sequence.
ACCESSION   DASUNT010000367 DASUNT010000000
VERSION     DASUNT010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927909"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VFX94_07425"
     CDS             complement(<1..927)
                     /locus_tag="VFX94_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HEX5768046.1"
                     /translation="MYEEVTVMRSNSGNNGRHEDVLKSLAQFSKEHRAARWAGTFASF
                     LETVFPADARGIARTSHQYTWDMMRAEGFEDANGRLRCQLFEGELYGINDTIERVVDY
                     FKAAAAGSEVGRRLLLLLGPPSGGKSTMVILLKRGLEEYSHTDAGALYGIQGCPVHES
                     PLHLVPHTLRGQFRSTYSVDITGELCPYCATRVQHDFGGDFMQVPVERIFISEAGRVG
                     VGTYAPHDPSTADIADLVGSVDLSKVAEFGDEGDPRAWSWSGAVFAASRGILEMIEIL
                     KVKREFLYLLLTLTQEKNVKVSRFPLIHLDETI"
     gene            1196..1546
                     /locus_tag="VFX94_07430"
     CDS             1196..1546
                     /locus_tag="VFX94_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768047.1"
                     /translation="MLKEILGVADDPPAKRRWFHDEYFDLFVWQADGEVTLFQLCYGG
                     DASERALVWDKQRGFFHDGPASRDVVALFEDAAAALPADLRAAIREKVHEFAGLQLSV
                     VSRRKKFRRASWQR"
     gene            1571..1978
                     /locus_tag="VFX94_07435"
     CDS             1571..1978
                     /locus_tag="VFX94_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX5768048.1"
                     /translation="MKAELFWLLCAVALTFVQMLLAVGGATLQVGLPALAGNREGLPA
                     FSGWAGRAQRAHRNMLENLVLFAALVLIAVVAGKTSSTTLLGAQLFFWARLAYAVVYI
                     AGVPWLRTLLWTVSVIGLALIFVPLALPSPVPR"
     gene            complement(1935..>2432)
                     /locus_tag="VFX94_07440"
     CDS             complement(1935..>2432)
                     /locus_tag="VFX94_07440"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5768049.1"
                     /translation="LVTAADLEGMLKAQGLSKRGVLPGDVVYVRTGWGEHWRDPDTEK
                     FYYSKAPGLSFDAAQWLAGRRIVLIALDTPFVDTVAEGLLQGKAGPAPGTPPGLPFAV
                     HHHMLAQAGIHLLENAKLDELAAERVWTSCTMILPLRERGAAGSAVRPVAIGAPGTAA
                     RAARK"
BASE COUNT          381 a          803 c          828 g          420 t
ORIGIN      
        1 gatggtctcg tcgaggtgga tcagcgggaa gcgcgacacc ttgacgttct tttcctgcgt
       61 gagcgtgagc agcaggtaca ggaactcgcg cttcaccttg aggatctcga tcatctcgag
      121 aatcccgcgg ctcgcggcga ataccgcgcc cgaccacgac caggcgcgcg ggtcgccctc
      181 gtcgccgaac tcggcaacct tggagaggtc caccgagccg accaggtcgg cgatatccgc
      241 ggtgctcggg tcgtgcggcg cgtacgtgcc cacgccgacg cgcccggcct cggagatgaa
      301 gatgcgctcg accggcacct gcatgaagtc gccgccgaag tcgtgctgca cgcgcgtggc
      361 gcaatacggg catagctcgc ccgtgatgtc cacgctgtag gtggagcgga actggccgcg
      421 cagcgtgtgc ggcacgaggt gcagcggcga ctcgtgcacc gggcagccct ggatgccgta
      481 gagcgcgccg gcgtccgtat ggctgtattc ctccagcccg cgcttgagga ggatcaccat
      541 ggtggacttg ccgcccgagg gcggcccgag cagcagcagc agccgccggc cgacttcgga
      601 tccggccgcg gccgccttga aatagtcgac cacgcgttcg atggtgtcgt tgatgccgta
      661 gagctcgccc tcgaagagct ggcagcgcag gcggccgttc gcgtcctcga agccctcggc
      721 gcgcatcatg tcccacgtgt actggtggct ggtgcgcgcg atgccgcgcg catccgcagg
      781 aaaaacggtc tcgaggaagc tcgcgaaagt gcctgcccag cgcgccgcgc gatgctcctt
      841 gctgaactgc gccagggact tgagcacgtc ctcgtggcgc ccgttgtttc cgctgttcga
      901 tcgcatcacg gtcacctctt catacatact gatcctctca acatcttaga gcaagcaaga
      961 atatgtatcc gcaactgcgt cgcctaccat cccgcggagc aagtaacgca ccgtccaacg
     1021 ggtctacgag aattcggttg acgtaataga atgggcgcgc gacgggaagt ttcccgacag
     1081 cagcgcgcgc ggtcggcgcc ggttcactgc gccgcgggaa agcggctgct accgccggtc
     1141 atgattcggt gacagaacgg agcaacgccg ccacggtcaa aacgttgacg aaaacatgct
     1201 taaagaaatc ctaggcgtcg ccgacgaccc gccggccaag cggcgctggt tccacgacga
     1261 atatttcgat ctcttcgtct ggcaggccga cggcgaggtg acgctgttcc agctctgcta
     1321 cggcggcgac gcaagcgagc gcgccctggt atgggacaag cagcggggct tcttccatga
     1381 cggtcccgca tcgcgggatg tggttgcgct atttgaggac gccgccgccg cgctgcccgc
     1441 cgacctgcgc gccgcgatcc gcgagaaagt tcacgaattc gccgggctgc agctctcggt
     1501 ggtgtcgcgc cgcaagaagt tccgccgggc gtcctggcag cgctaagctt ctccttcagg
     1561 agggtattcc atgaaagccg agctcttctg gctgctgtgc gcggtcgcgc ttacgttcgt
     1621 gcagatgctg ctcgccgtgg gcggcgcaac cctgcaagtg gggctgcccg cgctcgccgg
     1681 taaccgggag gggctgcccg cgttcagcgg ctgggccggc cgggcgcagc gggcgcaccg
     1741 caatatgctc gagaacctgg tgcttttcgc cgctttggtg cttatcgcgg tggtggcggg
     1801 caagacgagc tctaccacgc tgctcggggc gcagctcttc ttctgggcaa ggcttgccta
     1861 cgccgtggtt tatatcgccg gagttccctg gctgcgcacg ctgctctgga ccgtgtcggt
     1921 gatcggcctc gcgctcattt tcgtgccgct cgcgctgccg tccccggtgc cccgatagcc
     1981 acgggccgga ccgccgagcc ggcggcgcct ctttcgcgca gcggcaggat catggtgcac
     2041 gaggtccaca cgcgctcggc cgcgagctcg tcgagcttcg cgttctcgag gaggtggatc
     2101 ccggcctggg cgagcatgtg gtggtgcacg gcgaacggca gcccgggcgg cgtgccgggc
     2161 gccgggccgg ccttgccctg gagcaggccc tcggccacgg tgtcgacgaa cggcgtgtcg
     2221 agcgcgatca gcacgatgcg gcgcccggcg agccactgcg ccgcgtcgaa ggagaggccg
     2281 ggcgccttgg agtaatagaa cttttcggtg tcgggatcgc gccagtgctc gccccagccg
     2341 gtgcgcacat agaccacgtc gccgggcagc accccgcgct tcgagagccc ctgggccttg
     2401 agcatccctt cgaggtcggc cgcggtcacg ag
//
LOCUS       DASUNT010000368         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_51562, whole genome shotgun sequence.
ACCESSION   DASUNT010000368 DASUNT010000000
VERSION     DASUNT010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51562"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1965)
                     /gene="ligD"
                     /locus_tag="VFX94_07445"
     CDS             complement(<1..1965)
                     /gene="ligD"
                     /locus_tag="VFX94_07445"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX5768050.1"
                     /translation="MTDRLERYRGMRDFDVTPEPRGRKVAPRKKGLRYYIQRHDATRL
                     HYDLRLELEGVLKSWAVPKGPSLDPADKRLAMQTEDHPLEYGEFEGVIPEEQYGAGEV
                     LLWDTGVWTPEDKDPAAALAKGRLHFRLEGEKLRGSWILTRTSRAEERSSWLLIKRHD
                     DAARAGSDITVERPESVKRAPRSTRRARRSELPQFVTPQLATLVSQPPKSGDWVYEVK
                     HDGYRMLCRCSGDEVQLFTRSGRDWTGKLPHLARELRKLALEGAWLDGEIVVLRPDGR
                     SSFQALQNAFEGGADANIVYYVFDAPYLEGRDLRQLPLRERKARLKKSLKTGNTVRFS
                     EDLSGSAKEVLEQACRLGLEGLIGKQADSVYVAGRTRSWIKLKCRLTQDFVIAGYTRP
                     GGSRHGFGALVLGVYERPGRLVYAGKVGTGFDEERLRTLMRKFTAYKRPDSPLANPPR
                     ERDIQWLRPVLVAQVEFTERTDDGVIRQGSFMGLRDDIPAKSVGMEKPQAAPESEPAR
                     GIRITHPDRLIWPALGIRKIDLVRYVEEIGEWFLPHVANRPLTLVRCPDGVEKECFYQ
                     RHLNMGARPGDVLTFKRLRSSKGYYIYVNTLQGVTSVVQNGAVEFHTWGASVPDATHP
                     DRITIDLDPDPDLPWRQVMEGATLTRALIEG"
     gene            2097..>2611
                     /locus_tag="VFX94_07450"
     CDS             2097..>2611
                     /locus_tag="VFX94_07450"
                     /inference="COORDINATES: protein motif:HMM:NF016827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX5768051.1"
                     /translation="MGPAIALRLRQHLTLTPQVQQALRLLQMSAMEFAQEMEQAVATN
                     PFLEENPDAPAPSQREASVEEVVLPHEGSGSSAPAEREQDEWGGVSDTQPTLQEHLRE
                     QLRMSRVSDRDRALAEMVVDSLDEDGYFKATFEELAALLPKEHEVMPEEFAAALKLVQ
                     SLDPPGVAARTL"
BASE COUNT          398 a          915 c          862 g          436 t
ORIGIN      
        1 cccctcgatc agcgcgcgcg tgagcgtcgc gccttccatg acctggcgcc acggcaggtc
       61 ggggtccggg tcgaggtcga tggtgatgcg gtccgggtgc gtcgcatcgg gcacgctcgc
      121 gccccaggta tggaactcga ccgcgccgtt ctgcaccaca ctggtcacgc cctgcagcgt
      181 gttcacatag atgtagtagc ccttgctcga gcgcagccgc ttgaaggtca gcacgtcgcc
      241 cgggcgcgcg cccatgttga ggtggcgctg atagaagcac tccttctcga cgccgtccgg
      301 gcatcgcacc agcgtgagcg gccggttcgc cacgtggggc aggaaccact cgccgatctc
      361 ctcgacatag cgcaccaggt cgatcttgcg gatgccgagc gccggccaga tgagccggtc
      421 gggatgcgtg atcctgatgc ctctcgccgg ctcgctctcc ggcgcggctt gcggcttctc
      481 catgccgacc gacttggccg ggatgtcatc gcgcagcccc atgaacgagc cctggcggat
      541 cacgccgtcg tcggtgcgct cggtgaactc gacttgagcg accaggaccg gccgcagcca
      601 ctggatgtcg cgctcgcggg gaggattcgc cagcggcgag tccggcctct tgtaagccgt
      661 gaacttgcgc atcaatgttc ttagccgctc ctcatcgaag cccgtgccga ccttgccggc
      721 gtacacgagc cggcccgggc gctcgtacac gccgagcacg agcgccccga agccatggcg
      781 cgagccgccg ggcctggtgt agccggcgat cacgaagtcc tgggtcaggc ggcacttgag
      841 cttgatccag ctgcgcgtgc ggccggcaac gtacaccgaa tcggcctgct tgccgatcag
      901 gccttcgagc ccgagcctgc acgcctgctc gaggacttcc ttggcgctgc cgctgaggtc
      961 ctcgctaaac cgcacggtat tgccggtctt gagagacttc ttcagccggg ccttgcgctc
     1021 cctgagcgga agctggcgca agtcgcgccc ctccaggtac ggcgcgtcga acacgtagta
     1081 gacgatgttc gcatcggcgc cgccttcgaa cgcgttctgc agcgcctgga agctcgagcg
     1141 cccgtcgggg cgcagcacca cgatctcgcc gtcgagccac gcaccctcga gcgcgagctt
     1201 cctcagctcg cgcgcgagat gcggcagctt gccggtccag tcgcgtcccg aacgggtaaa
     1261 gagctgcacc tcatcgccgc tgcaacggca aagcatccgg tacccgtcgt gcttgacctc
     1321 gtagacccaa tcgcccgact tcggcggctg gctcacgagc gtcgcgagct gcggcgtcac
     1381 gaactgcggc agctcgctgc gccgggcgcg ccgcgtgctc cgcggcgcgc gtttcacgct
     1441 ctcgggacgc tcgacggtga tgtccgagcc tgcgcgcgcc gcgtcatcgt gccgctttat
     1501 gagcagccac gacgagcgct cctccgcccg gctggtgcgc gtcaggatcc atgagccgcg
     1561 cagcttctcg ccttcgaggc gaaagtgcag ccgtcccttc gcgagcgccg ccgccgggtc
     1621 cttgtcctcg ggcgtccaca cgcccgtgtc ccagagcagg acctcgcccg cgccgtattg
     1681 ctcttccggg atcacgcctt cgaattcgcc gtactcgagg ggatgatcct cggtctgcat
     1741 ggcgaggcgc ttgtcggcgg gatcgaggct cgggcccttg ggcaccgccc aactcttcag
     1801 gacgccctcg agctccaggc gcaggtcgta gtgcagacgc gtggcgtcgt ggcgctggat
     1861 gtaatagcga agccccttct tgcgcggcgc gactttcctg ccacgcggct ccggcgtgac
     1921 gtcgaagtca cgcatcccgc ggtagcgctc gagccggtct gtcatctgcc ggcaacctgc
     1981 aaggcccgag cccgtacggt aattgctacg ctgcgcggga attattacag cgcccgcccg
     2041 tgagcccttg agcgcaggcc gcttggcatg gcctttgcag atccttcggg caaaccatgg
     2101 gtccggcaat agcactaaga ctgcggcagc acctcaccct cacgccgcag gtccagcagg
     2161 cgctcaggct gctgcagatg tctgcgatgg agttcgcgca ggagatggag caggccgtcg
     2221 ccaccaatcc ctttctcgag gaaaacccgg acgctccggc cccctcgcag cgcgaagctt
     2281 cggtggaaga agtcgttctg ccgcatgaag gctcgggaag ctcggcgccg gcagagcgcg
     2341 agcaggacga atggggcggc gtgtcggaca cgcagcccac gctgcaggag cacctgcgcg
     2401 agcagctgcg catgtcgcgc gtcagcgatc gcgaccgcgc gctcgcggag atggtggtcg
     2461 acagcctcga cgaggacggc tacttcaagg cgaccttcga ggagctcgcc gcgctgctgc
     2521 ccaaggagca cgaggtgatg ccggaggagt tcgcggccgc gctcaagctc gtgcagagcc
     2581 tggacccgcc cggcgttgca gcccgcacgc t
//
LOCUS       DASUNT010000369         4700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1192511, whole genome shotgun sequence.
ACCESSION   DASUNT010000369 DASUNT010000000
VERSION     DASUNT010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192511"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1475
                     /locus_tag="VFX94_07455"
     CDS             183..1475
                     /locus_tag="VFX94_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5768052.1"
                     /translation="METGVLEHVANYPVIRKLGEGATSEVFLCQDPFRGRQVAVKRIF
                     PESLRDPARGRLFRKLFFTEASLAGKLDHPHIAQIYDAGVGDDAGYIVMEYVPGGTIE
                     RFCTPDTLLPVEQTVEIVFKCARALAYAHMQGVTHRDIKPGNLLYAAAPTDVRISDFG
                     LALNVGSETTQITGVGSPAYMSPEQIREELVDHRTDLYSLGVVMYQMLTGKLPFQGSN
                     KFSIIYQITQHEPPPPSSHRREVPEALDRIVRRAMQKDVTARYHSGDEFADELVGALR
                     STPGAARRESFGDADKFAALRALAFFRRFAEPELWEVVRIGQWQRVAAGTDIVREGEP
                     GDFFCVLLEGEARVTKNRKLLSVLGAGECFGEMAYLCAERRQRGATVTASKDAHIVRV
                     RTADLERASIGCRAHFDRAFVALLVERLNLANTRLTSA"
     gene            1535..2284
                     /locus_tag="VFX94_07460"
     CDS             1535..2284
                     /locus_tag="VFX94_07460"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEX5768053.1"
                     /translation="MHRCYAVVIAGRRRFLKANSPRQEDAFAAEADGLRALRNAGLRA
                     PEALAHGIAGDEAYLLLEYLELGSAGDFAALGRMLASAHRRPAPRFGWARDNYIGSTP
                     QKNAWCDDWAEFWLTRRWQPQLEWARGKGVNLPTIDPLPSLRNHKPQPSLLHGDLWSG
                     NAGFTKEGPVVFDPAVYYGDREADLAMTELFGGFPREFYQAYNETFPLDAGYEKRKHL
                     YNLYHLLNHLNLFGGGYLGQVKSTLSLLGAL"
     gene            complement(2281..2598)
                     /gene="grxD"
                     /locus_tag="VFX94_07465"
     CDS             complement(2281..2598)
                     /gene="grxD"
                     /locus_tag="VFX94_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX5768054.1"
                     /translation="MDTQDRIKQQVTGNRVVLYMKGTPQFPQCGFSALAVQVLNACGV
                     KDFHTVNVLADQEIREGIKQYAKWPTIPQLYVNGEFVGGSDIMREMYQSGELQKLLET
                     EKA"
     gene            complement(2608..3396)
                     /gene="prmC"
                     /locus_tag="VFX94_07470"
     CDS             complement(2608..3396)
                     /gene="prmC"
                     /locus_tag="VFX94_07470"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX5768055.1"
                     /translation="MTVQEALRSSGIDPREARLLLAAATGFSEASVLAFQERSLPGEA
                     ENRFTEFVSRRRTGDPVAYILGRKEFYGLDLAVNPAVLIPRPETELLVDLALQEAFSR
                     AVDLGTGCGAIALALKKHRPQASVIGVEASAAALVVARRNATKHSLEIDFRHGRWLEP
                     LGSEYCDLIVSNPPYVAAGDPHLAELRFEPAAALVGGPDGLDAIREIARAAPAHLAPG
                     GRLLIEHGMGQDRQVRELLSAAGLRGVQSWPDLAAIPRVSGGTR"
     gene            complement(3393..4478)
                     /gene="prfA"
                     /locus_tag="VFX94_07475"
     CDS             complement(3393..4478)
                     /gene="prfA"
                     /locus_tag="VFX94_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX5768056.1"
                     /translation="MNPRLRTKLDGMVGRLAELDGLLAAENATRDLEQFKRLSREHAE
                     ASSLVALFQSYLQVERDAAEAREMAADPAMKAYADEELKKARAAMERLEGELQTALLP
                     KDPNDERNLFIEVRAGTGGDESGLFAGDLFRMYTRYAERKGWQVEIISESASELGGYK
                     EVIARIAGQGAFSRLKFESGGHRVQRVPKTEAQGRIHTSACTVAVLPEADTIDEVVLN
                     PAELRIDTFRASGAGGQHVNKTESAIRVTHLPTGIVVECQDSRSQHKNREKALSVLAA
                     RIRDKQEREQQQKMASTRKSLIGSGDRSERIRTYNFPQGRVTDHRINLTLYKIDRIMD
                     GELDELIDALAHEHQAEQLASLTEEAA"
     gene            complement(4475..>4700)
                     /locus_tag="VFX94_07480"
     CDS             complement(4475..>4700)
                     /locus_tag="VFX94_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768057.1"
                     /translation="HWMRLREGVPLIRALRDEADAARREELERALKALERGENAAQVL
                     EALSRKLTNKLMHGPTQALTETLARLNRSK"
BASE COUNT          727 a         1614 c         1561 g          798 t
ORIGIN      
        1 actgtagccg gtcatccctc ctgccggtgg agcgcatcgt tcagatcatt ctcaaatgca
       61 cgcgggcact ccaattcgcg cacacgctcg gcgcgacgca ccgcgacagc tcgcccgccg
      121 ccgacgcagg catcatctcc atcccgacac gccggctcga ccaggcgtcg gagaattgag
      181 agttggaaac cggcgttttg gagcacgtcg ccaattaccc ggtgatccgc aagctgggcg
      241 aaggcgcaac ctccgaagtt ttcctctgcc aggacccgtt tcgcggccgc caggtggcag
      301 tgaagcgcat atttcccgag tcgctgcgcg accccgcccg cggccgactc ttcaggaagc
      361 ttttctttac cgaggcctcg ctcgcgggca agctcgacca tccgcacatc gcgcagatct
      421 acgacgccgg cgtgggggac gacgcgggct acatcgtcat ggagtatgtg ccgggcggga
      481 cgatcgagcg cttttgcact cccgacaccc tcctgccggt cgagcagacg gtggagatcg
      541 tcttcaagtg cgcgcgcgcc ctcgcctatg cgcacatgca gggcgtcacg caccgcgaca
      601 tcaagcccgg caacctgctc tacgccgccg cgccgaccga cgtgcgcatc agcgacttcg
      661 ggcttgcgct caacgtcggc tccgagacca cccagatcac cggcgtgggc tcgccggcct
      721 acatgtcgcc cgagcagatc cgcgaagagc tcgtcgatca ccgcaccgac ctctactcgc
      781 tcggcgtggt gatgtaccag atgctgaccg ggaagctccc attccagggc tcgaacaagt
      841 tcagcatcat ctaccagatc acccagcacg agccgccgcc gccgtcgtcg caccggcgcg
      901 aggtgcccga ggcgctcgac cggatcgtgc gccgtgccat gcaaaaagac gtgacggccc
      961 gctaccacag cggcgacgag ttcgccgacg agctggtcgg cgcgctgcgc agcacgcccg
     1021 gcgccgcgcg ccgcgaaagc ttcggcgatg cggacaagtt cgccgcgctg cgcgcgctgg
     1081 ccttcttcag gcgcttcgcc gagcccgagc tctgggaagt ggtgcgcatc gggcagtggc
     1141 agcgtgtcgc cgccggcacc gacatcgtgc gcgaggggga gccgggcgac ttcttttgcg
     1201 tgctcctcga gggcgaagcg cgcgtcacca agaatcgcaa gctcctcagc gtgctcggcg
     1261 ccggcgaatg cttcggcgag atggcctacc tctgcgccga gcggcgtcag cgcggcgcca
     1321 ctgtgaccgc ttcgaaagac gcacacatcg tgcgcgtgcg caccgccgac ctcgagcgcg
     1381 cctcgatcgg ctgccgcgcc cacttcgacc gcgccttcgt cgccctcctg gtcgagcggc
     1441 tcaacctcgc gaacacgagg ctgacatcgg cctaggcgcg gcgctgctgc acgagcttcg
     1501 gatcgagcag gccgccccgg catccggagg ctgcatccac cgctgctacg ccgtcgtcat
     1561 tgccggcagg cgccgctttc tcaaggcgaa ttcgccccgc caggaagacg cgttcgccgc
     1621 tgaagccgat gggctgcgcg ccctgcgcaa tgccggcttg cgcgcgccag aggcgctcgc
     1681 ccatggcatc gcgggtgacg aggcatacct gctgctggaa tatctcgagc tcggaagcgc
     1741 cggcgacttc gccgcgctcg gccgcatgct cgccagcgcg catcgcaggc cagccccgcg
     1801 cttcggctgg gcgcgcgaca actacatcgg ctcgacgccg cagaagaacg cctggtgcga
     1861 cgactgggcg gagttctggc tgacgcggcg atggcagccg cagttggaat gggcgcgcgg
     1921 gaagggcgtc aatctcccga cgatcgaccc gttgccttcc ctccgaaacc acaagcccca
     1981 accgtcgctg ctgcacggcg acctgtggag cggcaacgcg ggctttacga aggaagggcc
     2041 ggtggtgttc gacccggcgg tgtactacgg cgatcgcgag gcggatctcg cgatgaccga
     2101 gctattcggc ggatttccgc gcgagtttta ccaggcctac aacgaaacgt tcccgctgga
     2161 cgcgggatac gagaagcgca agcacctgta caacctctac cacctgctca accacctcaa
     2221 ccttttcggc ggtggctacc tggggcaggt gaagtcgacg ctcagccttc tcggggccct
     2281 ctaggccttt tccgtttcta gcagcttctg cagctcgccc gactggtaca tctcgcgcat
     2341 gatgtcggag ccgccgacga actcgccgtt cacgtagagt tgcgggatgg tcggccactt
     2401 ggcgtactgc ttgatgcctt cgcggatttc ctggtcggcg aggacgttca cggtgtggaa
     2461 gtccttcacg ccgcaggcgt tcagcacctg cacggcgagc gccgagaagc cgcattgcgg
     2521 aaactgcggc gtgcctttca tgtagagcac gacgcggttg ccggtgacct gctgcttgat
     2581 tctgtcttga gtgtccatgg cgcgatttta tcgcgttccc ccgctcaccc gcgggattgc
     2641 ggcgaggtcg ggccagctct gcacgcctcg aaggcctgcc gccgaaagga gctcgcgcac
     2701 ctgccggtcc tgccccatgc catgctcgat caggagccgc ccgccgggcg cgaggtgcgc
     2761 tggcgcggcc ctggctatct ccctgatggc atcgaggcca tcagggccgc cgacaagagc
     2821 ggctgccggc tcgaagcgaa gctcggcgag gtgcgggtcg ccggcggcga cgtaaggagg
     2881 gttcgagaca atgagatcgc aatactctga gccgagcggc tcgagccagc ggccgtggcg
     2941 aaaatcgatc tcgagcgaat gcttcgtcgc attgcgcctc gccaccacga gggctgccgc
     3001 gctcgcttcc accccgatca cgctcgcttg cgggcgatgc tttttcaagg cgagcgcgat
     3061 ggcgccgcag ccggtgccga gatccaccgc gcgcgagaaa gcctcttgca gcgcgagatc
     3121 gacgagcagc tcggtctcgg gacggggaat gaggaccgcg gggttcaccg cgagatcgag
     3181 cccgtagaac tccttgcgac cgaggatgta ggccaccggg tctccggttc gcctgcgcga
     3241 cacgaactcg gtgaatcgat tctcggcttc cccgggaagc gatcgttcct ggaacgcgag
     3301 cacgcttgct tcggagaatc cggttgccgc ggcaagcagc agccgcgcct cgcgcgggtc
     3361 gatgccgctc gagcgcagcg cctcttgaac ggtcaagcgg cttcttcggt caggctcgcg
     3421 agctgctcgg cctgatgctc gtgcgcgagc gcgtcgatca gctcgtcgag ctcgccgtcc
     3481 atgatgcggt cgatcttgta gagcgtgagg ttgatgcggt ggtcggtgac gcggccctgg
     3541 gggaagttgt aggtgcggat gcgctccgag cggtcgcccg agccgatcag ggacttgcgc
     3601 gtcgaggcca tcttctgctg ctgctcgcgc tcctgcttgt cgcggatgcg cgccgcgagc
     3661 acggagagcg ccttctcgcg gttcttgtgc tgcgagcgcg agtcctggca ctccaccacg
     3721 atgccggtcg gcaggtgggt aacgcggatg gcggactcgg tcttgttcac atgctggccg
     3781 ccggcaccgg aggcgcggaa ggtgtcgatg cgaagctcgg cgggattcag caccacctcg
     3841 tcgatcgtgt cggcttcggg aagcacggcc accgtgcagg cggaggtgtg gatgcgtccc
     3901 tgcgcttcgg tcttcggcac gcgctgcacg cggtggccgc ccgattcgaa cttgagccgg
     3961 gagaacgcgc cctggccggc gatgcgcgcg atgacctctt tgtagccgcc gagctcggag
     4021 gcgctctcgg agatgatctc cacctgccag cccttgcgct cggcatagcg cgtgtacatg
     4081 cggaaaaggt cgcccgcgaa aagcccggat tcatcaccgc cggtgccggc gcgcacctcg
     4141 atgaagaggt tgcgctcgtc gttcgggtcc ttgggcagga gcgcggtctg gagctcccct
     4201 tccagccgct ccatcgccgc gcgcgctttc ttcagctcct cgtcggcgta ggccttcatc
     4261 gccgggtcgg ccgccatctc gcgcgcctcg gcagcatcgc gctccacctg aaggtagctc
     4321 tggaaaagag cgaccagcga ggacgcttcg gcgtgctcgc gcgaaagccg cttgaactgc
     4381 tccaggtcgc gcgtggcgtt ctcggcggcg agcaggccgt cgagctcggc gagccgcccg
     4441 accatgccgt cgagcttggt gcgcaagcgg ggattcactt cgagcggttg aggcgcgcga
     4501 gagtctccgt gagcgcctgc gtcgggccgt gcatcagctt gttggtgagc ttgcgcgaca
     4561 gcgcctcgag cacctgcgcc gcgttttcgc cccgctcgag cgccttcaag gcgcgctcga
     4621 gctcctcgcg ccgcgccgcg tcggcctcgt cgcgcagcgc gcggatgagc ggcacgccct
     4681 cgcgcagccg catccagtgc
//
LOCUS       DASUNT010000370         6492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_928730, whole genome shotgun sequence.
ACCESSION   DASUNT010000370 DASUNT010000000
VERSION     DASUNT010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928730"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFX94_07485"
     CDS             <1..531
                     /locus_tag="VFX94_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX5768058.1"
                     /translation="TPVCTTELGYMARVKGEFDRRDTKIIGLSADSVEDHRRWAKDIE
                     ETQGAAVNYPMIGDEDLKVAKLYGMLPAEAAPGKRTPAENATVRTVFVIGPDKKIKLM
                     LFYPMTTGRNFDEIVRVLDSLQLTAYRKVATPVNWRQGGEVIIAGSVGDEEARKLFPQ
                     GWRAPRPYLRITRLAS"
     gene            complement(512..1537)
                     /locus_tag="VFX94_07490"
     CDS             complement(512..1537)
                     /locus_tag="VFX94_07490"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX5768059.1"
                     /translation="MLDHAPMLRSRSFDETRAYLKASHDIDMELVGRPGYDRSVDVRL
                     NALFLPAQWVAYLNYGAAARLRLSRDSSPWLARDIERKRLPAVYWVHFSLQGSLQAVI
                     RGQAFDGDQYRGLVLSPSGDKVLHTSPHNARLSLAVREETLNRHLAALLGREPCEPVE
                     FAPAISAASGHGRRLAGILHWAAAESERGAVLGHALVASRFEGFVLDALLLFQPHNYS
                     EALRCVERRLSPRDVRRARDYIHEHLASPITLADLVEASGVPGRTLLQHFRDAHGVSP
                     MRYVRNLRMERVRSELAGASVGCVAGCALRWGFAHAGRFAIEYRRRFGESPSRTLARA
                     RASLRAS"
     gene            complement(1651..2739)
                     /locus_tag="VFX94_07495"
     CDS             complement(1651..2739)
                     /locus_tag="VFX94_07495"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX5768060.1"
                     /translation="MLETTIAGSLPKPGWLAETNKLWPAWRAQGAELQRAKRDATLLW
                     LKEQEDAGVDIVTDGEQSRQHFVHGFLEFVEGIDFEHKVKMGIRNNRYDAMVPVVVGP
                     LRLRGRVHENEARLLRAHTRKKLKITMPGPMTIVDTIADRHYGDKVKLAMAFAALLNQ
                     EARALEKDGVDVIQFDEPAFNVYMDEVKRWGIEALHRAIEGLKCKTCVHICYGYGIPA
                     NIDWKKTLGDSWRQYEEIFPALAKSRIDQVSLECIHSKVPVELLRLLRGKDVLVGVID
                     VASDAVETAREVSEVIAKALKYVPARHLYPCTNCGMAPMDRDIAVHKLQALAAGAALA
                     RKRVAAAERTVGWGSGKLGKSTRNRGRR"
     gene            2787..3008
                     /locus_tag="VFX94_07500"
     CDS             2787..3008
                     /locus_tag="VFX94_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768061.1"
                     /translation="MLGMVVGSFCSFIAGLFNPYVANKAGPDWMWAGGRDDLVRGLYF
                     RPDGSFRRYGRIALLGTLLAGSATLYWIV"
     gene            3086..3316
                     /locus_tag="VFX94_07505"
     CDS             3086..3316
                     /locus_tag="VFX94_07505"
                     /inference="COORDINATES: protein motif:HMM:NF018369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HEX5768062.1"
                     /translation="MGKLIAAALVAGLVIAGPALAQRKDCGELRTEIEAKIKANGVEK
                     FSLDVVDKDAQAEGKVVGTCDGGTKKIVYKRA"
     gene            complement(3313..4536)
                     /locus_tag="VFX94_07510"
     CDS             complement(3313..4536)
                     /locus_tag="VFX94_07510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5768063.1"
                     /translation="MAASVPANAMVVVFCPFAAGYYLSYFYRYVNAVIAKDLVQDFGL
                     APSDLGLLTGAYFLSFAFAQLPLGVALDRFGPRRCTAALFCIAGAGAVTFALARDLAM
                     LSAGRALIGLGVSAGLMGSIKAFTLWFPRERLTSLSGWMIGIGALGALSATAPVEALL
                     GPYGWRALFLALGVLSVAAAALIFFVVPERALPGVGEPWREQLAVIGRIFARLDFWRL
                     AAPLVICQATFQALQGLWFAPWLSDVHGFGRGEAADYLFFSAAAYMLASFALGPFSEW
                     AGKRGVSQLRIYQAGMVLCSGAFAPLAFGAAGGVLFFLLAFAATSIAAIVAYTLLTQL
                     VPTAQTGRVTTASNLLLFATSFAFQWGVGAILGNWPSALGRYDPDGYRVAFGFLLAAQ
                     AAAAAWLLTARETQR"
     gene            4535..5125
                     /locus_tag="VFX94_07515"
     CDS             4535..5125
                     /locus_tag="VFX94_07515"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEX5768064.1"
                     /translation="MKKRLIVGITGATGAVYGVEILKTLRENPEWESHLVLSDAGALN
                     VWHELKLKRKELEKLADSAYHPKDIAATISSGSYLTEGMVIAPCSMKTLAAVAHAHAD
                     DLVARAADVVLKERRRLVLLPRETPLNLAHLRNMVAVTEMGGIVFPPLPAFYAAPKSL
                     EDLVAHTCARVLDLFGVHSARLARWQGMKEIPEERG"
     gene            complement(5073..5864)
                     /gene="pcaD"
                     /locus_tag="VFX94_07520"
     CDS             complement(5073..5864)
                     /gene="pcaD"
                     /locus_tag="VFX94_07520"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HEX5768065.1"
                     /translation="MKAKVNGIEIYYELHGKEDAPWLVLSHSLACSVRMWDPQIEAFK
                     ASHRILAYDMRGHGRTSAPAGAYTLDMLADDVLGLVASLAIKSAIYCGLSIGGMIGQT
                     LAIRGAAPFERMVLADTTHAQPPEALKQWEDRIRIAETKGMAALVESTLERWFTPPFR
                     TSAAARKIAEQIAATPVAGYVGCGRAIMALNTTAKLKSIKLPVLAITGEQDLAAPGTR
                     YIGENVPEAKLVVIPNASHIANIEQPEAFNRALREFLSSPASARA"
     gene            complement(5861..>6492)
                     /locus_tag="VFX94_07525"
     CDS             complement(5861..>6492)
                     /locus_tag="VFX94_07525"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5768066.1"
                     /translation="GAQRVAYETLDELVAQVEAAVKSGAERKFIYAYWPVYDMVSHRY
                     GAGSAEAFGEFSKMDAAFGELLARLAGTDSVVIATADHGFIDVAPEESLELPPSLASL
                     LRFPLCGERRVAYCHVHDPAAFIEKARDWLGERADVLPSRRLVDEGWLGPGAPHPRID
                     ERIGDVVLVMRGRYTVKDWTPGESRHLHIGNHGGTSEDEMLIPLILEAT"
BASE COUNT         1153 a         2129 c         2209 g         1001 t
ORIGIN      
        1 acgcccgtct gcaccaccga gctcggctac atggcgcgcg tcaagggcga gttcgaccgg
       61 cgggacacga agatcatcgg cctttcggcc gactcggtgg aggatcaccg gcgctgggcg
      121 aaagacatcg aggagacgca aggcgcggcg gtcaactacc cgatgatcgg tgacgaggac
      181 ctcaaggtcg cgaagctgta cggcatgctc cccgccgagg ccgcccctgg caagcgaacg
      241 ccggcggaga acgcgaccgt gcgcacggtg ttcgtgatcg gccccgacaa gaagatcaag
      301 ttgatgctgt tctacccgat gacgacggga cgcaatttcg acgagatcgt ccgggtgctc
      361 gactcgctgc agctcacggc ataccgcaag gtcgcgaccc cggtgaactg gcgccagggc
      421 ggcgaggtga tcatcgccgg ctcggtcggc gacgaagagg cgagaaagct ctttccgcag
      481 ggctggaggg cgccgcggcc ctacctgcgc attacgaggc tcgcaagcta gctctcgccc
      541 gggcgagggt gcgcgaagga ctctcgccga agcgcctgcg gtactcgatg gcgaagcggc
      601 cggcatgcgc gaagccccag cgcagcgcgc agccggccac gcacccgacg ctcgcgccgg
      661 cgagctccga gcgcacgcgc tccatccgga ggttgcgaac atagcgcatc ggggacacgc
      721 cgtgcgcgtc gcgaaaatgc tggagcagcg tgcgaccggg gacgccggag gcctccacca
      781 ggtcggcgag cgtgatcggg gaggcgagat gctcgtggat gtagtcgcgg gcgcgcctca
      841 cgtcgcgcgg cgaaaggcgg cgctcgacgc aacgcagcgc ctcgctatag ttgtggggct
      901 ggaaaaggag cagcgcatcg agcacgaaac cctcgaagcg cgaggcgacg agcgcgtggc
      961 ccaggaccgc gccgcgctcc gactcggcgg ccgcccagtg cagtatcccg gcgagccgcc
     1021 gcccatgtcc gctggcggcg ctgatggccg gtgcgaactc caccggctcg cacggctcgc
     1081 ggccaaggag cgcggcgaga tgccggttaa gcgtctcttc ccgcaccgcg agcgacagcc
     1141 gggcattgtg gggcgaggta tgcaggacct tgtccccgga aggcgacagc acgaggccgc
     1201 gatactggtc gccgtcgaac gcctgcccgc ggatcaccgc ctggaggctg ccctgcagcg
     1261 agaagtgaac ccagtagacc gccggcagcc gcttgcgctc gatgtcgcgc gcgagccagg
     1321 ggctcgagtc ccgggatagc ctcaggcggg cggccgcgcc gtagttcagg tacgccaccc
     1381 actgggcggg caggaaaagc gcgttcagac gcacgtcgac cgacctgtca tagccgggcc
     1441 gcccgacgag ctccatatcg atgtcgtggc tcgccttcag gtaggcgcgc gtctcgtcga
     1501 agctgcgcga gcgaagcatc ggcgcgtggt caagcattgc cccaacatag cagccgagcg
     1561 ttttcctccg gtttccgcca tgtttctgga acgtaacagg gcccagagct gcggtttccg
     1621 gacagccgcc cggtgcgccc ctcttgcctt ctagcggcga cccctgttgc gagtgctctt
     1681 gccgagcttg ccggagcccc accccaccgt gcgttccgcg gcggcaacgc gcttgcgcgc
     1741 gagcgcggcg cccgcggcga gggcttgcag cttgtgcacg gcgatgtcgc ggtccatcgg
     1801 cgccatgccg cagttggtgc aagggtagag atgccgggcg ggcacgtact tgagggcctt
     1861 ggcgatcacc tcggagactt cccgcgcagt ctcgactgca tcggaggcga cgtcgatcac
     1921 gccgaccagc acgtccttgc ccctgagaag cctcaggagc tcgaccggga ctttggagtg
     1981 gatgcattcg agggacacct ggtcgatcct gctcttcgca agcgcgggga agatttcttc
     2041 gtactggcgc cacgagtcgc caagggtctt cttccagtcg atgttcgccg ggatgccgta
     2101 cccgtagcag atgtgcacgc aggtcttgca cttcaggccc tcgatcgcgc ggtgcagggc
     2161 ttcgatgccc cagcgcttga cctcatccat gtagacgttg aatgccggct cgtcgaactg
     2221 gatgacgtcg acgccgtcct tctcgagcgc gcgtgcttcc tggttgagca gcgccgcgaa
     2281 cgccatggcg agctttacct tgtcgccgta gtgccgatcg gcgatggtgt cgacgatcgt
     2341 catcgggccg ggcatggtga ttttcagctt tttcctggta tgcgccctta gcagacgggc
     2401 ttcgttttcg tgcacgcgtc cgcggagtcg aaggggcccg acgaccaccg gcaccatggc
     2461 gtcgtagcgg ttgttgcgga tgcccatctt caccttgtgc tcgaagtcga tgccctcgac
     2521 gaactcgagg aacccatgta cgaagtgctg gcgcgactgc tcgccatcgg tgacgatatc
     2581 caccccggca tcttcctgct ccttaagcca aaggagcgtg gcgtcgcgct tcgcgcgctg
     2641 gagctcggcg ccctgggcac gccacgccgg ccacagcttg ttggtttcgg cgagccagcc
     2701 cggcttcgga aggcttccgg cgatcgtggt ttcaagcatg gtgacgcctc actataatcg
     2761 agcgcgcaac aagggaggga agacgcgtgc tcggcatggt ggtgggaagc ttttgctcgt
     2821 tcatcgcagg actattcaat ccgtacgtgg cgaacaaggc cggcccggac tggatgtggg
     2881 ccggcggcag ggacgacctg gtgcgcggcc tctacttccg ccccgacggc agtttccggc
     2941 gctacggccg catcgcgctt ctcgggacgc tccttgccgg gtcggcgacc ctctactgga
     3001 tcgtctgact gccaagcatt tcttttgcaa agtgtcagga atcaagtcat tctaggagtc
     3061 ccaagcacca aaaaagggag caaacatggg gaagctgatt gctgcggcgc tggtggcggg
     3121 tctcgtcatc gcgggaccgg ccttggctca gcgcaaggac tgcggcgagc tgaggaccga
     3181 aatcgaggcc aagatcaagg cgaacggcgt ggagaaattt tcgctcgacg tcgtggacaa
     3241 ggacgcccag gccgaaggta aggtggtcgg cacctgcgac ggcggcacga agaagatcgt
     3301 ctacaagcgc gcctagcgct gagtttcccg tgcagtaagc agccaggccg cagccgcggc
     3361 ctgcgctgca agcaggaatc cgaaggcgac gcgatagccg tcgggatcat accggccgag
     3421 cgcgctcggc cagttgccca gaattgcgcc gacaccccat tggaaggcga acgaggtggc
     3481 gaaaaggagg agattcgacg ccgtcgttac gcgcccggtc tgcgcggtcg ggacgagctg
     3541 cgtaagcagc gtgtaggcca cgatcgctgc gatgctggtc gccgcgaacg ccagcaggaa
     3601 gaacaggacc ccgccggcgg cgccgaaagc gagcggcgcg aaggcgccgc tgcagagcac
     3661 catgccggcc tggtagatcc ttagctgcga tacgcctcgc ttgccggccc attcggagaa
     3721 aggtcccagg gcaaaggagg cgagcatgta cgccgcggcg gagaagaaca ggtaatccgc
     3781 ggcctcaccc cggccgaaac cgtggacgtc cgagagccac ggcgcgaacc agagcccttg
     3841 cagcgcctgg aatgtcgcct ggcagatcac gagaggcgcc gcgagccgcc agaaatcgag
     3901 gcgcgcgaag atcctgccga tgacggcgag ctgctcgcgc cagggctcgc ccaccccggg
     3961 cagcgctcgc tcggggacga cgaagaagat gagcgccgct gccgcgacgc tcagcacgcc
     4021 gagcgcgagg aagagcgccc gccagccgta aggaccgagc aacgcctcga ccggcgcggt
     4081 ggccgatagc gcgccgagcg cgccgatgcc gatcatccag ccgctcagcg aagtgaggcg
     4141 ctcgcgcgga aaccacaggg tgaacgcctt gatggagccc atcagcccgg ccgatacgcc
     4201 aaggccgatg agcgcgcgcc cggcagagag catcgcaagg tcgcgcgcga gcgcgaacgt
     4261 caccgcgccc gcgccggcga tgcagaagag cgccgcagtg cagcgccgcg ggccaaaccg
     4321 atcgagcgca acgccgagcg ggagctgcgc aaaagcgaac gaaaggaaat acgcgcccgt
     4381 gagaaggccg aggtccgagg gcgcaagccc gaagtcctgc accaggtcct tcgcgatgac
     4441 cgcgttgacg tagcggtaga agtaggagag gtaatagccg gccgcgaacg ggcagaacac
     4501 cacaaccatg gcgttcgcgg gtacgctagc cgccatgaag aagcggctga tcgtcggaat
     4561 aacgggagcg accggggccg tctatggggt cgaaatactg aagaccctgc gcgaaaaccc
     4621 cgagtgggaa agccatctcg ttctctcgga cgccggagcg ctcaacgtct ggcacgagct
     4681 caagttgaag cgcaaggagc tcgagaagct tgccgactcc gcctaccacc cgaaagacat
     4741 cgccgcgacc atctcgagcg gctcttacct cacagagggc atggtcatcg cgccctgctc
     4801 catgaagacc ctcgccgccg tggcgcacgc gcatgccgac gatctcgtgg cgcgggccgc
     4861 cgacgtggtg ctgaaggagc ggcgccggct cgtgcttctg ccgcgcgaga cgcccctcaa
     4921 cctcgcgcac ctgcgcaaca tggtcgcggt caccgagatg ggcgggatcg tatttccgcc
     4981 gctgcccgcg ttctacgcgg cgccaaagtc gctcgaggac ctcgtcgcgc acacttgcgc
     5041 gcgcgtgctc gacctcttcg gcgtgcactc cgctaggctc gcgcgctggc aggggatgaa
     5101 agaaattccc gaagagcgcg gttgaaagcc tccggctgct cgatgttggc gatgtgcgag
     5161 gcgttcggga tcacgacgag cttcgcttcc gggacgttct ccccgatgta gcgcgtgccc
     5221 ggcgccgcca ggtcctgctc gccggtgatg gcgagcaccg gcagcttgat gctcttcagc
     5281 ttcgcagtcg tgttgagcgc catgatggca cgcccgcaac cgacgtagcc cgcgaccggc
     5341 gtggcggcga tctgctcggc gatcttccgg gccgcggcgc tcgtgcggaa gggcggcgtg
     5401 aaccagcgct ccagggtcga ctcgacaagc gcggccatgc ctttggtctc ggcgatgcgg
     5461 atgcgatcct cccactgctt gagcgcttcc ggcggctgcg cgtgcgtcgt gtcggcgagc
     5521 accatgcgct cgaagggcgc cgcgccgcgg atggcgaggg tctggccgat catgccgccg
     5581 atcgacagcc cgcagtagat ggcgctcttg atcgccagcg aagccacgag accgagaaca
     5641 tcgtcggcca gcatgtcgag cgtataagct cccgccggcg cgctcgtgcg cccatgcccg
     5701 cgcatgtcgt aggcgaggat gcggtggctt gccttgaacg cttcgatctg cgggtcccac
     5761 atgcgcacgc tgcacgcgag cgaatggctg agaaccagcc agggcgcgtc ttccttgcca
     5821 tgcagctcgt agtagatttc gatgccattg acctttgcct tcatgtcgcc tccaggatga
     5881 gcggaatcag catttcgtcc tcgctcgtgc cgccatggtt gccgatgtgc aggtggcgcg
     5941 attcgcccgg cgtccagtcc ttgaccgtgt agcggccgcg catgacgagc acgacgtcgc
     6001 cgatgcgctc gtcgatgcgc gggtgcgggg cacccggacc caaccagccc tcgtccacca
     6061 ggcggcggct cggcaggacg tcggcgcgct cgcccagcca gtcccgggct ttctcgatga
     6121 aagccgcagg atcatggacg tggcagtaag ccacgcgtcg ttcgccacac agaggaaacc
     6181 gcaagagaga agcaagcgat ggcggcagct cgagagattc ctcgggcgcg acgtcgatga
     6241 agccgtggtc cgcggtcgcg atcaccacgg agtcggtgcc ggcgaggcgc gcgaggagct
     6301 cgccgaacgc tgcatccatt ttgctgaact cgccgaacgc ttcggcgctg cccgcgccgt
     6361 agcggtgcga caccatgtcg tacaccggcc agtaggcgta gatgaacttg cgctccgcgc
     6421 ccgacttcac cgcggcctcg acctgcgcca ccagctcgtc gagcgtctca tacgccacgc
     6481 gctgtgcgcc gc
//
LOCUS       DASUNT010000371         2902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1192570, whole genome shotgun sequence.
ACCESSION   DASUNT010000371 DASUNT010000000
VERSION     DASUNT010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192570"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2566)
                     /gene="icmF"
                     /locus_tag="VFX94_07530"
     CDS             complement(<1..2566)
                     /gene="icmF"
                     /locus_tag="VFX94_07530"
                     /EC_number="3.6.5.-"
                     /EC_number="5.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0047727 - isobutyryl-CoA mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fused isobutyryl-CoA mutase/GTPase IcmF"
                     /protein_id="HEX5768067.1"
                     /translation="MTDLSDSSKLVYKAKQKIRFVTAASLFDGHDASINIMRRILQAS
                     GAEVIHLGHNRSVDEVVSAALQEDVQGIAVSSYQGGHLEYFKYMLDLLRAQGGEHIKV
                     FGGGGGVIVPAEIKELEARGVARIYSPEDGQRMGLQGMINDMLARCDFDPSRKDPKTL
                     AQVITALELGVFRDKTSLHEKAKANPAPVLGITGTGGAGKSSLTDELVRRFRLDQGNE
                     LKIAVLSVDPTRRKTGGALLGDRIRMNSIHGPSVFMRSLATRDTGTELSAALGDAIAA
                     CRAAGYGLVIVETAGIGQGDAAIVPHVDASLYVMTPEFGAASQLEKIDMLDFADFVAI
                     NKFDRRGADDALRDVRKQVQRNRELFARPPEDMPVFGTIAARFNDDGVTALYHAVADK
                     LGLRRQPGTLDRPKSKVSSAVHVVVPPKRQRYLAEIAETVRGYHQHAGEQSRLARERQ
                     QVESTRAMLGKGFPELEALLESRQLDAESKKLLETWPKTVKAYSGDEQVLKVRGKEVR
                     TRLTHTSLSGTHVPKVALPKYEDHGELLRWRLRENLPGEFPFTAGVFHFKRENEDPTR
                     MFAGEGDPFRTNRRFHLLSKDMPAKRLSTAFDSVTLYGFDPDERPDIYGKVGNSGVSI
                     ATLDDMKVLYSGFDLCDPSTSVSMTINGPAPTILAMFFNTAIDQQVEKLGREPTEKEL
                     AEIKAKTLSAVRGTVQADILKEDQGQNTCIFSTEFSLKVMGDIQQYFIDNQVKNFYSV
                     SISGYHIAEAGANPISQLAFTLANGFTFVEAYLARGMKVDDFAPNLSFFFSYGMDPEY
                     AVLGRVARRIWAVAMRERYGANERSQKLKFHSQTSGRSLHAQEIAFNDIRTTLQALI"
     gene            complement(2613..>2902)
                     /locus_tag="VFX94_07535"
     CDS             complement(2613..>2902)
                     /locus_tag="VFX94_07535"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5768068.1"
                     /translation="SPLLPDAPTMAEAGLKDLSVTPWGGVFGPKGLPRDVTDRVSREL
                     QAVLKRPEVREAFGKLAFEPRGSTPEELAAFTREQLAVWRRAIADAGIKPE"
BASE COUNT          465 a          939 c          964 g          534 t
ORIGIN      
        1 agatcagcgc ctgcagcgtg gtgcggatgt cgttgaaggc gatctcctgc gcgtgcagcg
       61 agcggccgga ggtctgcgag tggaacttga gcttctggct gcgctcgttg gcgccgtagc
      121 gctcgcgcat ggccaccgcc cagatgcgcc gcgccacgcg accgagcacc gcgtattccg
      181 ggtccatgcc gtacgagaag aagaacgaaa gattgggcgc gaagtcgtcg accttcatgc
      241 cgcgcgcgag gtaggcctcg acgaaggtga agccgttggc gagggtgaac gcgagctgcg
      301 agatcgggtt ggcgccggcc tcggcgatgt gatagcccga gatcgacacc gagtagaagt
      361 tcttcacctg gttgtcgatg aagtactgct ggatgtcgcc catcaccttg agcgagaact
      421 cggtggagaa gatgcaggtg ttctgcccct ggtcttcctt caggatgtcg gcctgcaccg
      481 tgccgcggac ggccgacagg gtcttggctt taatttctgc aagctctttt tccgtcggct
      541 cgcgaccgag cttctccacc tgctgatcga tcgcggtgtt gaagaacatc gccaggatcg
      601 tgggcgccgg cccgttgatg gtcatcgaca ccgaagtcga cggatcgcaa aggtcgaagc
      661 ccgagtacag cactttcatg tcgtcgagcg tggcgatcga cacgccgctg ttgccgacct
      721 tgccgtagat gtccggccgc tcgtccggat cgaagccgta gagcgtcacc gagtcgaagg
      781 cggtcgacag gcgcttcgcc ggcatgtcct tcgagaggag gtggaagcgc cggttggtgc
      841 ggaagggatc gccctcgccg gcgaacatgc gcgtcgggtc ctcgttttcg cgcttgaagt
      901 ggaacacccc ggcggtgaac gggaactcgc cgggcaggtt ctcgcgcagg cgccagcgca
      961 gcaattcgcc gtgatcctcg tacttcggca gcgccacctt ggggacgtgc gtgccggaga
     1021 gcgaggtatg ggtgagccgc gtacgcacct ccttgccgcg cactttcaac acctgctcgt
     1081 cgcccgaata ggccttcacc gtcttcggcc aggtctcaag gagctttttc gattccgcat
     1141 ccagctgcct cgattcgagc agggcttcga gctcggggaa gcccttgccg agcattgccc
     1201 gggtcgattc aacttgctgc cgctcgcgcg ccaacctcga ttgctctcca gcgtgttggt
     1261 ggtaaccccg caccgtttcc gcgatctcgg cgaggtagcg ctgccgcttc ggcggcacga
     1321 ccacgtgcac ggcggaagag actttgctct tgggtctgtc gagcgtccct ggctggcgtc
     1381 tcaaccccaa tttgtccgcg accgcgtggt agagcgccgt gaccccgtcg tcgttgaagc
     1441 gcgcggcgat ggtgccgaac accggcatgt cctcgggcgg gcgcgcgaac agctcccggt
     1501 tgcgctgcac ctgcttgcgc acgtcgcgca gcgcgtcgtc ggcgccgcgg cggtcgaact
     1561 tgttgatcgc gacgaagtcg gcgaagtcga gcatgtcgat cttctcgagc tggctcgccg
     1621 cgccgaactc gggcgtcatc acgtagaggg aggcgtccac gtgcggcacg atcgcggcgt
     1681 cgccctggcc gatgccggcg gtctcgacga tcacgagccc gtagcccgcg gcgcggcacg
     1741 ccgcgatcgc atcgccgagc gccgccgaga gctccgtgcc cgtgtcgcgc gtggcgagcg
     1801 aacgcatgaa gacgctcggc ccgtggatcg aattcatgcg gatgcggtcg ccgagaagcg
     1861 cgccgccggt cttgcggcgc gtcgggtcca cggaaaggac ggcgatcttg agctcgttgc
     1921 cctggtcgag gcgaaagcgc cgcacgagct cgtcggtgag cgagctctta cccgcgccac
     1981 cggtgccggt aatgcccagc acgggcgcag gattggcctt tgctttttcg tgaagcgacg
     2041 tcttgtcccg gaaaacccca agctcgagcg ccgtgattac ctgcgcgagc gtcttcgggt
     2101 ctttgcgcga aggatcgaaa tcacagcgcg ccagcatgtc gttgatcatg ccctgcaggc
     2161 ccatgcgctg cccgtcctcg ggcgaataga tgcgcgcgac gccgcgcgcc tcgagctcct
     2221 tgatctcggc cggcacgatc acgccgccgc cgccgccgaa caccttgatg tgctccccgc
     2281 cctgcgcccg caaaaggtcc agcatgtatt tgaagtactc gaggtggccg ccctggtagg
     2341 acgagaccgc gatgccctgc acgtcctcct gcagcgccgc gctcaccacc tcgtcgaccg
     2401 agcggttgtg accgaggtgg atcacctccg cccccgaagc ctgcaggatg cggcgcatga
     2461 tgttgatcga cgcgtcgtga ccgtcgaaca ggctcgccgc ggtgacgaag cggatctttt
     2521 gctttgcctt gtagacaagc ttgctcgagt ccgagaggtc ggtcacggaa ccggtagcct
     2581 agttaacgtt tacgttaacg tcaatcagcc attcactcgg gcttgatacc ggcgtccgcg
     2641 attgcgcggc gccagaccgc aagctgctcg cgggtaaacg cggcgagctc ctcaggggtg
     2701 gagccgcgcg gctcgaaggc gagcttgccg aaggcctcgc gcacttccgg gcgcttgagg
     2761 acggcctgca actcgcgcga gacgcgatcg gtgacatcgc gcggcaggcc tttggggccg
     2821 aatacgccgc cccacggcgt gacgctcaga tctttcaacc ccgcttccgc catcgtcggt
     2881 gcgtcgggca acagcgggct gc
//
LOCUS       DASUNT010000372         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_841827, whole genome shotgun sequence.
ACCESSION   DASUNT010000372 DASUNT010000000
VERSION     DASUNT010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841827"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VFX94_07540"
     CDS             <1..801
                     /locus_tag="VFX94_07540"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX5768069.1"
                     /translation="PPDAPARAFELHGGAGRVMLDPRWYQAAATFLASGFRHILEGAD
                     HLLFLLCLVLPYRRLGWSLVAVITSFTVAHSITLIAAARGMVPSGSWFPPLVEVLIAA
                     SILYMALENIVRPNLRSRWLITGLFGLVHGFGFAFLLREQFQFAGEHLLLSLLAFNGG
                     IELGQLVFIGVVIPVLFFLKDKLRLDARLTSIVVSAFIAHSAWHWLAERWEQLAKQPA
                     PQVEIAQLAPWLVLGTALALLGWALLGPRLGRMTVLRRSDPEAGVKPH"
     gene            complement(742..1638)
                     /locus_tag="VFX94_07545"
     CDS             complement(742..1638)
                     /locus_tag="VFX94_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX5768070.1"
                     /translation="MLGASADSLRGIPARYAVATGAQPEFLMEGAGGLFAAKWRVPPH
                     RVANLWAPDHVLGYRVSGIANIVRRCRGVIKRRVPPIGSVSFTPGDRPIEWTSDAPIE
                     TLHVYIDAQALRGFSEGHLDGAEVQIDDFFGIVEPWLSGFCGLLDSDARYDADSLFLD
                     QTQHLLLRHLVRHHSNAAPGLRRALEPRARVAPLGRPVVRRIEDYLERNMSVDVSLHT
                     LAALAHLSVDHFVRAFRAATGQTPHRFALERRLERAAAMLKGPATIAQIAHDCGFRSP
                     AHFSVRFHARFGVTPSQYRHSA"
     gene            complement(1790..>2151)
                     /locus_tag="VFX94_07550"
     CDS             complement(1790..>2151)
                     /locus_tag="VFX94_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX5768071.1"
                     /translation="RLPRLAVLGDLNIAPEPRDVHDPKRWEGKIHFSEPERAAFQEVV
                     QTGLKDAFRLFDQPEKQFTWWDYRLKAFERGWGLRIDHILLARELAVVCKACAIDTEP
                     RKRERPSDHAPVIAELH"
BASE COUNT          312 a          722 c          732 g          385 t
ORIGIN      
        1 ccgccggatg ctccggcgcg tgccttcgag ctgcacggcg gagcgggccg ggtcatgctc
       61 gacccgcgct ggtaccaggc tgccgccacg ttcctcgcgt cgggcttccg ccacatcctg
      121 gagggcgccg accacctgct cttcctgctg tgcctcgtgc tgccctatcg ccgcctcggc
      181 tggtcgctgg tcgcggtgat tacctcgttc accgtggcgc actcgatcac gctcatcgcc
      241 gcggcgcgcg gcatggtgcc ctccggcagc tggttcccgc cgctggtgga ggtgctgatc
      301 gccgcgtcga tcctctacat ggcgcttgag aacatcgtgc gcccgaacct gcgctcgcgc
      361 tggctgatca ccgggctgtt cggtctggtg cacggcttcg gattcgcgtt cctgctgcgc
      421 gagcagttcc agttcgccgg cgagcacctc ctgctctcgc tcctcgcctt taacgggggg
      481 atcgagcttg gccagctcgt cttcatcggc gtagtcatac cggtcctgtt ctttctcaag
      541 gataagttgc ggctcgacgc ccggctcacg agcatcgtgg tctcggcgtt catcgcgcac
      601 tcggcgtggc actggcttgc cgagcgctgg gaacagctgg cgaagcagcc cgcgccacaa
      661 gttgagatcg ctcagctcgc gccgtggctg gtccttggga cggcgctcgc attgctcggc
      721 tgggcgctgc tcggcccgcg tctaggcaga atgacggtat tgcgaaggag tgaccccgaa
      781 gcgggcgtga aaccgcactg agaaatgcgc cgggctgcgg aagccgcaat cgtgcgcgat
      841 ctgcgcgatc gtcgccgggc ccttgagcat cgccgcggcg cgctcgagcc gccgctcgag
      901 cgcgaagcgg tgcggcgttt ggccggtggc ggcgcggaaa gcgcgcacga agtggtcgac
      961 cgacaggtgg gcgagcgccg cgagcgtgtg gagcgagaca tcgacgctca tgttcctctc
     1021 gaggtagtcc tcaatgcgcc gcacgaccgg gcggccaagg ggcgcgacgc gcgcgcgcgg
     1081 ctcgagcgcg cggcgcagcc cgggcgcggc gttcgagtgg tggcgcacca agtgccgcag
     1141 gagcaggtgc tgcgtctggt cgaggaacag cgaatcggcg tcgtaccggg catcgctgtc
     1201 gagcagtccg cagaagcctg acagccaggg ctcgacgatg ccgaagaagt cgtcgatctg
     1261 gacctcagcg ccgtccagat gcccctcgga gaagccacgc agcgcctgcg cgtcgatgta
     1321 cacgtgcagc gtctcgatcg gcgcgtcgct cgtccactcg atcggccggt cgcccggggt
     1381 aaagctgacc gagccgatcg gcggcaccct gcgttttatg acgccccggc acctgcgcac
     1441 gatgttcgcg atcccgctca cgcggtagcc cagcacatgg tccggggccc acagattcgc
     1501 cacgcggtgc ggcggcactc gccacttggc cgcaaacagt ccgccggcgc cttccatcag
     1561 gaattccggc tgcgccccgg tcgccacggc gtaccgcgcc gggatgcccc tcaacgagtc
     1621 ggcgctagcg ccaagcatcg cgctctcttg aacggttgcg ggtgcgacgg cgcacgcgag
     1681 cgtgcggggg actttcactt tcgacttgcc ttgcgtgttg cagctgctat tgaaccgcaa
     1741 caggccaccc gtgcgctatt agcagtcttg ggtagactcc tgatgcagtc tagtgaagct
     1801 ctgcaatcac gggggcgtga tctgaagggc gttcccgctt tcgcggttcg gtgtcaatgg
     1861 cacaagcctt gcacacgacg gcaagctcgc gagcgagcag gatatggtcg atgcgcagcc
     1921 cccacccgcg ctcgaaggcc ttcaggcggt agtcccacca ggtgaactgc ttttcgggct
     1981 ggtcgaagag gcggaacgcg tcctttaggc ccgtttggac tacttcctgg aaggcggcgc
     2041 gctctggctc ggaaaagtgg atcttgcctt cccagcgctt ggggtcgtgg acgtcccgcg
     2101 gctcaggcgc gatgttcaag tctccgagaa ccgccagccg cggcaggcgc g
//
LOCUS       DASUNT010000373         2046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_841908, whole genome shotgun sequence.
ACCESSION   DASUNT010000373 DASUNT010000000
VERSION     DASUNT010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841908"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VFX94_07555"
     CDS             <1..332
                     /locus_tag="VFX94_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768072.1"
                     /translation="EHAPRMRAVLASLDEAAALAEKRAGLVVLMQANPFFTFPRDGFA
                     DLRNSLERLAARSPQKVVLIHGDTHVYRYDEPLPGLRRIEVWGSPIVSWIRASASPGA
                     LRIDAVR"
     gene            complement(343..1722)
                     /locus_tag="VFX94_07560"
     CDS             complement(343..1722)
                     /locus_tag="VFX94_07560"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX5768073.1"
                     /translation="MKQGLATLVSFIAIVVATLLALEPATSQDAARDPGLRPGPAGAG
                     GPIDGLTRNELRFFQHGKEDFEEVEEVDEGLGPRMNLDSCGGCHSQPAIGGSSPARNP
                     QVAFAQAANPSQYPAVQAFIKPDGPVREARFVRNADGTSDGGVHALFTITGRDGADGG
                     CSLRQPDFAGEIQRGNVIFRIPTAMFGAGLIEQIPDAAILDNMQANAAEKAALGIRGK
                     PNLAVSGRTLFGSTNRNDNDGTVARFGWKAQNQSLLIFSGEAYNVEMGITSEMFQSER
                     EQAKGCQYAQVPNDTQDFNAGDALTGTTAIQNFANFQRFLAPPKPSPDTPGGSASIAR
                     GRGAFASVGCALCHTPSLRTSDKATVFALRNREVKLFSDLVLHDMGARLADGVSQGSA
                     GPSEFRTPPLWGLGQRIFFLHDGRTSDLKVAIQEHASEGSEANGVVGRYNELREDQKQ
                     DLLNFLRSL"
     gene            1834..>2046
                     /locus_tag="VFX94_07565"
     CDS             1834..>2046
                     /locus_tag="VFX94_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5768074.1"
                     /translation="MDDWRRTATPRAFIDKPAAVNCDSNRKTRIDFGEGGDRLLHLRD
                     LHCFMQVYETRSFSKAADSLDTVQSLV"
BASE COUNT          331 a          679 c          675 g          361 t
ORIGIN      
        1 ccgagcatgc gccgcgcatg cgcgcagtgc tcgcgtcgct cgacgaggcg gccgcgcttg
       61 ccgagaagcg cgccggactc gtcgtcctga tgcaagcgaa cccgttcttt actttcccgc
      121 gcgacggctt cgccgatctt aggaacagcc tcgagcggct agccgcccga tccccgcaga
      181 aagtcgtcct gatccacggc gacacccacg tctaccgtta tgacgagccg ctccccggat
      241 tgaggcgcat cgaggtatgg ggctccccca tcgtgagctg gatccgcgcc tcggcatcgc
      301 ccggggcgct gcgcatcgac gccgtgcgct gagcctgcgc cgctacagcg agcgcaggaa
      361 gttgagcagg tcctgcttct ggtcctcgcg aagctcgttg tagcggccga ccacgccgtt
      421 cgcctcggag ccttcgctcg cgtgctcctg gatcgcgacc ttcaagtccg aagtacgccc
      481 gtcgtgcagg aagaagatcc gctgccccag cccccacagc ggcggggtgc ggaactcgct
      541 cgggcccgcg ctcccctggc tcacgccgtc ggcaagccgg gcgcccatgt cgtgcagcac
      601 gaggtcggaa aagagcttca cctcgcggtt gcgcagcgcg aagaccgtcg ccttgtccga
      661 tgtgcgcagg ctcggcgtat ggcacagcgc acagccgacg ctggcaaaag cgccgcgccc
      721 gcgcgcgatc gacgcggagc ctcccggcgt atcgggcgag ggctttggcg gcgcgaggaa
      781 gcgctggaag ttggcgaagt tctggatcgc cgtggtgccg gtgagggcgt cgccggcgtt
      841 gaaatcctgc gtgtcgttag ggacctgcgc gtactggcat cccttcgcct gctcacgctc
      901 cgactggaac atctcgctgg taatgcccat ttcgacgttg tacgcctcgc cggagaaaat
      961 aagcagcgac tggttctgcg ccttccagcc gaagcgcgcg acggtgccgt cgttgtcgtt
     1021 gcggttcgtc gagccgaaga gggtgcgtcc ggagaccgcc aggttgggct tgccgcggat
     1081 cccgagcgcg gccttttcgg ccgcgttcgc ctgcatgttg tcgaggatcg ccgcgtccgg
     1141 gatctgctcg atcagccccg cgccgaacat cgccgtcgga atgcggaaga tcacgttgcc
     1201 gcgctggatc tcgcccgcga aatccggctg ccgcaggctg catccgccgt cagcgccatc
     1261 gcggccggtg atcgtgaaaa gcgcgtgcac gccgccgtcg gacgttccgt cggcgttgcg
     1321 cacgaagcgc gcttcgcgca ccgggccgtc cggcttgatg aaggcttgaa cggcgggata
     1381 ctgcgatgga ttcgccgcct gggcgaacgc tacttgagga ttgcgcgcag ggctcgagcc
     1441 gccgatcgcg ggctgcgaat ggcagccgcc gcagctgtcg aggttcatcc tcggcccgag
     1501 gccttcgtcg acctcttcga cctcctcgaa atcctccttg ccgtgctgga agaacctaag
     1561 ctcgttccgc gtgaggccgt cgatcggccc gccggcgccg gccggcccgg gacgcaggcc
     1621 gggatcgcgc gctgcatcct ggctggtggc cggctcgagc gcgagcagcg tggcgacaac
     1681 gatggcgatg aaggaaacaa gtgtggccag gccttgcttc atggcgtccc gctccttccc
     1741 ctggaatagc tgtagtgccg gtggcgacgc gggttcatca cagcacagcg caaagccgcg
     1801 ctcaatcagc gaacactgat atctcggatt gcgatggacg actggcgccg cacggcgaca
     1861 cctcgcgctt tcattgacaa accggctgca gtgaattgtg atagcaatcg aaaaaccaga
     1921 attgacttcg gagaaggagg tgatcgattg cttcacctca gggatttgca ctgcttcatg
     1981 caggtgtatg agacgcgaag cttcagcaaa gccgctgaca gcctcgacac ggttcagtcg
     2041 ctggtc
//
LOCUS       DASUNT010000374         5504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1193211, whole genome shotgun sequence.
ACCESSION   DASUNT010000374 DASUNT010000000
VERSION     DASUNT010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193211"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..964
                     /locus_tag="VFX94_07570"
     CDS             95..964
                     /locus_tag="VFX94_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5768075.1"
                     /translation="MRVALFLILALAARAADATHEPWSTTRFPQDEDKWWWDADWWER
                     GEIAVPQNHTVRVRAASYRNGEVEIPVQIFSPGRFPVVVFLHGRRGLDELTRLVPLRL
                     AARGFTVVAPDLFGGRFIESMPVRHDPALEDDAALAIDFALALPEARGQAKTCVVSHT
                     RGGYYALKALVTKGRQAKAACYVSYYPHWQHPEAPEAMQIYQYAAEVDELRVPVLIFV
                     GEHEQYQRVRPILAGIDSLKARKRDARVVVYPGVGRGFDFRPRDVRTLADDLAAQDSL
                     TRAAAFIRLRLSP"
     gene            975..1406
                     /locus_tag="VFX94_07575"
     CDS             975..1406
                     /locus_tag="VFX94_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX5768076.1"
                     /translation="MRAKQVLAMLVVAIVAGCAGTPGSDKRDKVVYHVNEGMAQASSG
                     LRNINNHLEVNPEARIVVVTHAQGVDFLMKGAKDANGNKYEDLVERLKQRGVQFDVCD
                     ITLRNRKLSRDQFIEYATFVPSGVAEVTRLQQREGYAYLRP"
     gene            1455..1898
                     /gene="soxY"
                     /locus_tag="VFX94_07580"
     CDS             1455..1898
                     /gene="soxY"
                     /locus_tag="VFX94_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HEX5768077.1"
                     /translation="MKRRGLLKSAAVLIAAGLLKPLAALAAWNKAAFSSKTSADALKS
                     LGTPATEPSAGILIEAPQIAENGAVVPIEITSNIPGTTSIAVLVEKNPYPLVGKFDFS
                     EGALPFVKINAKMGETSDVRVVATAGGKHYQATREIKVTIGGCGG"
     gene            1900..2196
                     /gene="soxZ"
                     /locus_tag="VFX94_07585"
     CDS             1900..2196
                     /gene="soxZ"
                     /locus_tag="VFX94_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HEX5768078.1"
                     /translation="MSGPMKMRATLAGNYTDVRVLMTHPMSTEQQTKGQPHFIQNVTV
                     KLNGKTVIDGEISQAVSRNPVFSFRLKGGAKGDKIEVSWLDNKGDTNRTETAVA"
     gene            2196..2987
                     /gene="soxA"
                     /locus_tag="VFX94_07590"
     CDS             2196..2987
                     /gene="soxA"
                     /locus_tag="VFX94_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HEX5768079.1"
                     /translation="MRAAAGIVIAAFACAGASAQQESQFADYQRMTAEGSPVELFELA
                     GEELWKKPQGPKNVSLEKCDLGKGPGVLKGAYAALPRYFKDADRVMDLETRLLHCMTT
                     LQGRTTAEATKRLYGNADTPSEMEYLSAYVAGQSRGAKLAPGSHHPKEKESYQLGKAV
                     FFHRTGSWDFSCASCHGEQGKRIRMSELPVLSEPKAAQPLIATWPAYRVSNSQFKTMQ
                     WRINDCYRQMRMPEPNFASDLTIALNMYLATTARGAEYRGPGTKR"
     gene            2984..3580
                     /gene="soxX"
                     /locus_tag="VFX94_07595"
     CDS             2984..3580
                     /gene="soxX"
                     /locus_tag="VFX94_07595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HEX5768080.1"
                     /translation="MSRAAFAILLGFCAGSTLAEPTSRDVTEAMKRDFHPRGQATMAR
                     IPQDGVQRICTETRDNPPAALARQLEADQMKTIVFPSGSLMGDWKRAERVAQSGRGSM
                     WNDQPGVDVDGSCYNCHQISPQETSYGTIGPSLLGFGKKRGNNAETQKYVYGKIYNAK
                     AYNACSQMPRLGYAGTLSEEQIKDLVALLLDPASPVNQ"
     gene            3582..5261
                     /gene="soxB"
                     /locus_tag="VFX94_07600"
     CDS             3582..5261
                     /gene="soxB"
                     /locus_tag="VFX94_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HEX5768081.1"
                     /translation="MHRREFLHCLALAAAAGATLRSGQSCAQASAELYELPAFGNVSL
                     LHITDVHAQLLPVHFREPSVNLGNGRPPHLVGEALLREFSIEQRSPLAHAFTHLHFAE
                     SARRYGKLGGYAHLATLVKRLRASRPHALLLDGGDSWQGSATALWTKGADMIGAQKLL
                     GVAAMTAHWEFTYGAEYMKQAVKSLEPIELLAHNVRTVDFEDPVFKPYSLRSVNGVAL
                     AVIGQAFPYTPIAHPRHLVAEWSFGIQENRLQSLVDEVRAKGAQAVVLLSHNGMGVDL
                     KLASRLRGIDAILGGHTHDGVPAPVVVGKTLVTNAGSNGKFLAVLDLKVSAGGVEDYR
                     YRLLPVFSNLLPPDAAMAAYVEKARAPFAAKLSEKLALTEGLLYRRGNFNGSWDELIL
                     QALLEQKDAEIAFSPGFRWGTTLLPGDAITLEHVMDQTAITYPFSTLSELTGAQIKTI
                     LEEVADNLFHPDPYLQQGGDMVRVGGMSYACEPGARIGSRISRMKLGEKELQPEKPYK
                     VAGWAPVAEGASGEPIWDVVSRHLRAKKTIAVLEPRLPRLVGVSGNPGLAR"
     gene            complement(5314..>5504)
                     /locus_tag="VFX94_07605"
     CDS             complement(5314..>5504)
                     /locus_tag="VFX94_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HEX5768082.1"
                     /translation="EDTANHVLGGILMGFGGITALGCTIGQAISGFSTLALGSIMTFV
                     AIVAGSAAAMKYQYWRAG"
BASE COUNT         1022 a         1831 c         1811 g          840 t
ORIGIN      
        1 ccagtcgggc acttcgttcg cgtgcgcagg cagtcccgcg gccacgatga acgccgcgca
       61 cagcctgaga actgcatttg tgattgaatg ggccatgcga gtcgcgctgt tcctcattct
      121 agcgctggcc gcccgggcgg cagacgcgac gcacgagccg tggagcacca cgcgcttccc
      181 ccaggatgaa gacaaatggt ggtgggacgc cgactggtgg gagcgcggcg agatcgccgt
      241 gccgcagaac cacacggtgc gcgtgcgcgc ggcgagctat cgcaacggcg aggtcgaaat
      301 cccggtccag attttttcgc ctggcagatt tccggtggtg gttttcctgc acggacggcg
      361 cggcctggac gagctcacgc ggctcgtgcc gctgcgcctt gcggcgcgcg gcttcaccgt
      421 ggtcgcgccc gatctcttcg gcggccgctt catcgaaagc atgccggtgc gccatgaccc
      481 ggcgctcgag gacgatgcgg cgctcgccat cgacttcgcg ctcgcgctgc ccgaggcgcg
      541 cggccaggcg aagacctgcg tcgtctcgca cacgcgcggc ggctattacg cgctcaaggc
      601 gctcgtcacg aaaggcaggc aggcgaaggc cgcttgctac gtctcttact atccgcactg
      661 gcagcacccg gaggcgccgg aggcgatgca gatctaccag tacgccgcgg aggtggacga
      721 gctgcgcgtg ccggtgctca tctttgtcgg cgagcacgag cagtaccagc gcgtgcgccc
      781 gatcctggcc ggcatcgatt cgctgaaggc caggaagcgg gatgcaaggg tggtggtcta
      841 ccccggcgtc gggcgcggct tcgatttccg gccgcgcgac gtgcgcacgc ttgccgacga
      901 cctcgcggcc caggactcgc tcacccgcgc cgccgccttc atccggctta gactatcgcc
      961 gtgaggacat tcccatgaga gcgaaacagg ttttggcgat gcttgtcgtc gcaatcgtcg
     1021 cgggctgcgc ggggacgccg ggttccgaca agcgcgacaa ggtcgtctac cacgtcaacg
     1081 aaggcatggc gcaggcctcg agcgggctgc gcaacatcaa caatcatctc gaggtcaacc
     1141 ccgaggcgag gatcgtcgtc gtgacgcacg cgcagggcgt ggacttcctg atgaagggcg
     1201 cgaaggatgc gaacggcaac aagtacgaag acctggtcga gcgcctcaag cagcgcggcg
     1261 tgcagttcga cgtctgcgac atcacgctgc gcaaccgcaa gctgagccgc gaccagttca
     1321 tcgagtacgc cactttcgtg ccctccgggg tcgccgaagt gacacggctg cagcagcgcg
     1381 agggctatgc gtacctgcgg ccctgaatgc ggttaccggt taccattgcg ggcccactga
     1441 ctataggtga cccgatgaag cgtaggggat tgctgaaaag cgcggcggtg ctgatcgccg
     1501 ccgggctgct gaagccgctc gcggccctcg ccgcctggaa caaggcggcg ttctcgtcga
     1561 agacgagcgc cgatgcgctg aagagcctgg gcacgccggc gacggagccg agcgcaggca
     1621 tcctgatcga ggcgccgcag atcgcggaga acggcgcggt cgtgccgatc gagatcacga
     1681 gcaacatccc cggaacgact tcgatcgcgg tgctggtcga gaagaacccg tacccgctcg
     1741 tggggaagtt cgacttctcg gaaggcgcgc tgcctttcgt caagatcaac gccaagatgg
     1801 gcgagacttc ggacgtgcgc gtggtcgcca ccgcgggcgg caagcactac caggcgacca
     1861 gggagatcaa agtgaccatc ggcgggtgcg gaggctgaca tgtcgggacc gatgaagatg
     1921 agagccaccc tcgccggcaa ctacaccgac gtgcgcgtgc tgatgaccca cccgatgtcg
     1981 accgagcagc agaccaaggg acagccgcac ttcatccaga acgtgacggt gaagctcaac
     2041 ggcaagacgg tgatcgacgg cgagatcagc caagccgtgt cgcgcaaccc cgtgttctcc
     2101 ttccgcctga agggcggggc gaagggcgac aagatcgaag tgagctggct ggacaacaag
     2161 ggcgacacca acaggaccga gacggcggtc gcctgatgcg cgccgccgca ggcattgtca
     2221 tcgcggcgtt tgcgtgcgcg ggcgcgtctg cgcagcagga atcgcagttc gccgactacc
     2281 agcgcatgac ggccgagggc agcccggtcg agcttttcga gctcgccggc gaggagctgt
     2341 ggaagaagcc ccagggcccg aagaacgttt cgctggagaa atgcgatctc ggcaagggac
     2401 cgggggtgct gaaaggcgcc tacgccgccc tgccgcgcta cttcaaggac gccgaccgcg
     2461 tcatggatct cgagacgcgg ctcctgcact gcatgaccac gctgcagggg cgcaccaccg
     2521 cggaggcgac caagcgcctg tacggcaacg ccgacacgcc ctcggagatg gaatacctct
     2581 cggcctacgt cgctggccag tcgcgcggcg cgaagttggc gccgggcagc caccacccca
     2641 aggagaagga aagctatcag ctgggaaagg cggtcttctt ccatcgcacc gggtcgtggg
     2701 acttctcgtg cgcctcctgc catggcgagc aaggcaagcg catccgcatg tccgagctgc
     2761 cggtgctgag tgaaccgaaa gcggcgcagc cgctgatcgc cacctggccg gcgtaccgcg
     2821 tgtcgaacag ccagttcaag accatgcagt ggcgcatcaa cgactgctac cggcagatgc
     2881 gcatgccgga gccgaatttc gcctccgatc tgacgatcgc cctgaacatg tacctcgcca
     2941 ccaccgccag gggcgccgaa taccgcggcc cgggcaccaa gcgatgagcc gtgccgcgtt
     3001 tgccatcctg ctcggctttt gcgcgggctc gactcttgcg gagcccacgt cccgcgacgt
     3061 cacggaagcg atgaagcggg acttccatcc gcgcggccag gcgacgatgg cacgcattcc
     3121 ccaggacggc gtgcagcgca tctgcaccga gacgcgcgac aatccgcccg ccgcgctcgc
     3181 gaggcagctc gaagccgacc agatgaagac catcgtgttc ccgtcgggca gcctgatggg
     3241 cgactggaag cgcgccgaaa gggtggcgca gagcggccgc ggcagcatgt ggaacgacca
     3301 gccgggcgtg gacgtcgacg gcagctgcta caactgccac cagatctcgc cgcaggaaac
     3361 ctcgtacggc acgatcggcc cgagcctgct cggcttcggc aagaaacgcg gcaacaacgc
     3421 cgagacgcag aagtatgtgt acggcaagat ctacaacgcc aaggcctaca acgcctgctc
     3481 gcagatgccg cgcctcggct acgccggaac gctgagcgag gagcagatca aggacctggt
     3541 cgcgctgctg ctcgatcccg cctcgccggt gaaccagtag gttgcaccgg cgcgaattcc
     3601 ttcactgcct tgcgctcgcc gccgccgcgg gcgccacgct gcgcagcggg cagtcctgcg
     3661 cccaggcttc agccgagctc tacgagctgc cggcattcgg caacgtttcg ctgcttcaca
     3721 tcacggatgt ccacgcgcag cttctcccgg ttcacttccg cgaacctagc gtaaacctcg
     3781 gtaacggaag accacctcac ctcgtcgggg aagcgcttct aagggaattc tccatcgagc
     3841 agcgaagccc gcttgcgcac gccttcaccc acctccactt cgcggagtcg gcccgccgct
     3901 acggcaagct gggcggctac gctcatctcg caactctggt gaagcggctg cgcgcctcgc
     3961 ggccgcacgc gcttcttctg gacggtggcg acagctggca gggctcggcc accgcgctat
     4021 ggaccaaggg cgcggacatg atcggcgcgc agaagctgct cggcgtagcg gccatgaccg
     4081 cccactggga atttacctac ggcgcggaat acatgaagca ggcggtgaaa agccttgaac
     4141 cgatagagct cctcgcacac aacgtgcgga ccgtggattt cgaggacccg gtcttcaagc
     4201 cctacagcct gcgcagcgtg aacggtgtcg cgcttgcggt gatcggccag gccttcccat
     4261 acacgccgat cgcccacccc cgccacctcg tcgccgagtg gagcttcggc attcaggaga
     4321 accgcctcca gagccttgtc gacgaggttc gcgcgaaggg cgcgcaagcc gtagtactgc
     4381 tctcgcacaa cgggatgggc gtcgacctga agctcgcttc gcgcttgcgc ggcatcgacg
     4441 cgatcctcgg cggccatacc cacgatggcg tgccggcgcc ggtggtggtg ggcaagacgc
     4501 tcgtcaccaa cgccggctcg aacggcaaat tcctcgccgt gctcgacctg aaagtatccg
     4561 caggcggggt ggaggactac cgctaccgcc ttctgccggt gttttccaac ctgctgcccc
     4621 ccgacgccgc catggccgct tacgtggaaa aagcgcgggc gccctttgcc gcaaagttga
     4681 gcgaaaaact tgccctcacc gaagggctgc tctaccgccg cggcaacttc aacggcagct
     4741 gggacgagct catcctgcag gcgctgctcg agcagaagga cgccgagatc gcgttttcgc
     4801 ccggcttccg ctggggcacg acgctgctcc ccggcgacgc catcacgctc gagcacgtga
     4861 tggaccagac cgcaatcacg tatcccttct cgacgctgag cgagctcacc ggcgcgcaga
     4921 taaagacgat cctcgaggag gtggcggaca acctcttcca tcccgatccc tatctccagc
     4981 agggcggcga catggtgcgc gtaggcggga tgagttatgc gtgcgaaccc ggggccagga
     5041 tcggctcacg catttcccgg atgaagctgg gagaaaaaga actccaaccc gaaaagccct
     5101 acaaggtcgc cggttgggcg ccggtcgcgg agggcgcgag cggcgagccg atatgggacg
     5161 tggtctcgcg ccacctgcgt gcgaagaaga caatcgcggt gctcgagcca cgcctaccgc
     5221 ggcttgtcgg cgtgtcgggc aaccccgggc tcgcgcgcta gggaaactct ggccgatagt
     5281 cttccccgcg caggcgggga agacgtattc ctcctagccg gcgcgccagt actggtactt
     5341 catcgccgcc gctgatccgg cgacgattgc gacgaacgtc atgatcgagc cgagcgcgag
     5401 cgtcgagaag cccgaaatcg cctgcccgat cgtgcagccg agggcggtga tgccgccgaa
     5461 tcccatgagg atgccgccga gcacgtggtt cgcagtgtcc tcgg
//
LOCUS       DASUNT010000375         2413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_317446, whole genome shotgun sequence.
ACCESSION   DASUNT010000375 DASUNT010000000
VERSION     DASUNT010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317446"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VFX94_07610"
     CDS             complement(<1..674)
                     /locus_tag="VFX94_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX5768083.1"
                     /translation="MRRVVFNHKGGVGKSTIACNLAAVSAKGGARVLVVDLDPQGNST
                     QYLLGKHGRELEPTLAGFFEQVLSFPLRPKKPEDFVHGTLFERLDVLPSHPLLAQQQD
                     KLEARYKIFKLRDALAELDRQYDFIYVDTPPALNFFTRSALIATDCCLIPFDCDNFSR
                     RALYTLLEDIAEIRADHNAGLVVEGIVVNQFLPRARLPQKMVQELIAEGLPVLQPYLS
                     SSVKIR"
     gene            853..1221
                     /locus_tag="VFX94_07615"
     CDS             853..1221
                     /locus_tag="VFX94_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEX5768084.1"
                     /translation="MKLHASRPSGVNTITGYGAGYVMVNGERRASSVVVLADRVEDWT
                     VENFEALSAEHFEFLKTLDIEILLLGTGPRQRFPHPRLTAALAQAGIGLEVMDVQAAC
                     RTYNILVAEDRKVAAALLFE"
     gene            1218..2090
                     /locus_tag="VFX94_07620"
     CDS             1218..2090
                     /locus_tag="VFX94_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5768085.1"
                     /translation="MSLLPGFIQKRIRTSGASINLAVGGEGPPVLLLHGYPQTHVMWH
                     KVAPSLARDYTVVCADLRGYGDSSKPRGIPGHGNYAKRAMALDMVEAMEALGFVKFHV
                     VGHDRGGRVAHRMARDHGRRVQTLTVLDISPTLKMYQSTNMQFAKAYYHWFLLIQEAP
                     LPEQMLRNMGPGYILMRLGRGKTGLKVFDRRALAEYARCFDPHSTCEDYRAAATIDLV
                     HDRKDLHRKVRMPLLALWGRQGVIQALFDCLADWREVALDVRGRALQCGHFIPEERPK
                     ELLAELCRFLGRNL"
     gene            2209..>2413
                     /locus_tag="VFX94_07625"
     CDS             2209..>2413
                     /locus_tag="VFX94_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX5768086.1"
                     /translation="MLGKPVPDFSLPSTGGGTFKLSSARGAKLVLYFYPKDHTPGCTT
                     EGADFRDLHGEFRTAGAEVFGVSR"
BASE COUNT          435 a          778 c          781 g          419 t
ORIGIN      
        1 tcccggatct tgaccgatga ggagagatag ggctggagca ccggcagacc ctcggcgatc
       61 agctcttgca ccatcttctg cggcagacgg gcgcgtggga ggaattggtt gactacgatg
      121 ccttcgacga ccagccccgc gttgtgatct gcgcgaattt cggcaatgtc ctcgagcagc
      181 gtgtaaagcg cccggcgcga aaaattgtcg caatcgaacg gaatgaggca gcaatcggtt
      241 gcgatgagcg ccgagcgcgt gaagaaattg agggcgggcg gcgtgtccac gtagatgaaa
      301 tcgtactgcc tgtccagctc ggcgagcgcg tcgcgcaact tgaagatctt gtagcgcgcc
      361 tcgagcttgt cctgctgctg ggcgagcagc ggatgcgagg gcagcacgtc gagccgctcg
      421 aacagcgtgc cgtgcacgaa gtcctccggc ttcttgggcc gcagcgggaa gctcagtacc
      481 tgctcgaaga agccggcgag cgtcggctcg agttcccggc cgtgcttgcc gaggaggtac
      541 tgggtcgagt tgccctgcgg gtcgaggtcg acgaccagga cgcgggcccc gcctttcgcg
      601 ctgactgcgg ccagattaca ggcgatcgtg gacttgccga cgcctccctt gtggttgaag
      661 acaacacgac gcatgctccc tcccttgcgg actggtgacc tgggcatcat agtcaaacga
      721 gatagcgttg ccaatgaggc gcggtctact agatctagta gggtcgtgtg cgatcgcgac
      781 gaccggcgcc aaccgggtgg agcgatacgc aaagcgccct tcgggcctcg cttcccctag
      841 tagaattccg gcgtgaagct ccacgcctcc cgcccgagcg gcgtcaacac gatcacggga
      901 tacggcgcag gctacgtcat ggtgaacggc gagcggcgcg cctccagcgt cgtggtgctt
      961 gccgaccgcg tcgaagactg gacggtggag aacttcgagg cgctcagcgc ggagcatttc
     1021 gagttcctga agaccctcga catcgagatt ttgctgctcg gcaccggccc gcggcagcgc
     1081 ttcccgcatc cccggctcac cgccgcgctc gcgcaggcgg gcatcgggct cgaagtgatg
     1141 gacgtgcagg ccgcctgccg cacctacaac atcctggtgg ccgaggaccg caaagtcgct
     1201 gccgcgctcc ttttcgaatg agccttcttc cggggttcat ccagaaaaga atccgcacga
     1261 gcggcgcgag catcaacctt gcagtcggag gcgagggtcc tccggttctg ctgctccacg
     1321 gctatccgca gactcacgtc atgtggcaca aggtcgcgcc gtcgcttgcg cgcgactaca
     1381 ccgtggtgtg cgcggacctg cgcggctacg gcgactcctc caagcctcgc ggcatccccg
     1441 gccacgggaa ctacgccaag cgcgcgatgg cgctcgacat ggtcgaggcc atggaagcgc
     1501 tcggcttcgt caagttccac gtcgtgggcc acgaccgcgg cggccgcgtc gcgcatcgca
     1561 tggctcgcga ccatggccgg cgcgtacaga ccctcaccgt gctcgacatc tcgccgacct
     1621 tgaagatgta ccagagcacg aacatgcagt tcgcgaaggc gtactaccac tggttcctcc
     1681 tcatccagga ggccccgctg cccgagcaga tgctgcgcaa catggggccg ggctacatcc
     1741 tcatgcgcct cgggcgcggg aagaccggct tgaaggtttt cgaccggcgg gcgcttgccg
     1801 agtacgcgcg gtgcttcgat ccgcactcga cgtgcgagga ctaccgcgcg gcggccacca
     1861 tcgatctcgt gcacgacaga aaagacctgc accgcaaagt gaggatgccg ctgctcgcgc
     1921 tctggggccg gcagggagtg atccaggcgc tcttcgactg cctcgccgac tggcgcgagg
     1981 tggcgctcga cgtgcgcggc cgcgcgctgc aatgcggcca cttcatccct gaggaaaggc
     2041 cgaaggagct tctcgccgag ctgtgccgct tcctcggccg caacctctag ggcttgaaaa
     2101 acctttctaa tcagggagca aggcctcgcg cttgcgcccg tagcacgact tgtgttcaat
     2161 gaaggcgtta cagcgcatta ctaagaaggg tattcaaaac ctctggaaat gctcggcaag
     2221 ccggtcccgg acttcagcct tcccagtacc ggcggcggca ccttcaagct ctcgagcgcg
     2281 cgcggagcca agctcgtcct gtatttctat ccgaaggacc acacgccggg gtgcaccacc
     2341 gagggcgcgg atttcagaga cctgcacggg gaattccgca cggccggcgc cgaggtgttc
     2401 ggagtttccc gcg
//
LOCUS       DASUNT010000376         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_666870, whole genome shotgun sequence.
ACCESSION   DASUNT010000376 DASUNT010000000
VERSION     DASUNT010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666870"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VFX94_07630"
     CDS             complement(<1..398)
                     /locus_tag="VFX94_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5768087.1"
                     /translation="MYCIVTGAAGFIGSKLVEGLNRRGITEVIAVDNLAHADKFRNLA
                     GAEIADYLDQAEFIGDLERFDGAVEALFHQGACSDTMETDGRYMLDNNYAYSRRLLEW
                     CQEEEVPLLYASSASVYGAGPDFREERRCE"
     gene            complement(402..1322)
                     /gene="rfaE1"
                     /locus_tag="VFX94_07635"
     CDS             complement(402..1322)
                     /gene="rfaE1"
                     /locus_tag="VFX94_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEX5768088.1"
                     /translation="MDTAKARVLVAGDVMLDRYWFGDVSRISPEAPVPVVLIREEDQR
                     LGGAANVAWNCKELGARTRLLSVVGRDEPGQALERLLKARRIEASLHRDRGLSTTQKL
                     RVIGRKQQLLRIDFEKPPSTEVLASKLEEFQRALADCDVVLLSDYGKGGLAHIVEMIR
                     AARRAGKRVLVDPKGDDYSRYRNASIITPNLAELREVIGTWKDEKDLKSRAQALREKL
                     GLEALLLTRGEDGMTLFARNRATTIKAEAREVFDVTGAGDTVIATLAVMLAAGARLEA
                     AVRLANRAAGIVVGKLGTAAVRHQELFGAR"
     gene            complement(1319..>2264)
                     /locus_tag="VFX94_07640"
     CDS             complement(1319..>2264)
                     /locus_tag="VFX94_07640"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX5768089.1"
                     /translation="DKVQAAIAEVLAKRGVSLRFSMVSNPEFLKEGAAVEDFMRPDRV
                     IIGADDERAVALLRQLYAPFQRNHERIMVMSVRSAELTKYAANSMLATRISFMNELAN
                     LAEAIGADIEEVRRGVGSDPRIGYQFLYAGAGFGGSCFPKDVKALQRSAAEAGKPLRV
                     LAAVEAVNEAQKHVLAEKVKARLGKDLRGRTIALWGLAFKANTDDMREAPSLTVVADL
                     VAAGASVRGYDPAAAEQARKIFAGENRVAVLDSAMAALEGADVLAIATEWQEFRSPDF
                     AAIRERLKTPAIFDGRNLYDPAALKALGLEYYPIGRRT"
BASE COUNT          336 a          809 c          728 g          391 t
ORIGIN      
        1 ttctcgcagc gccgctcctc gcggaagtcg ggcccggcgc cgtaaaccga ggccgaggag
       61 gcgtagagga gcggcacttc ctcctcctgg caccactcaa gcaaacgcct ggagtaggca
      121 tagttgttgt cgagcatgta gcgcccgtcg gtctccatcg tgtccgagca cgcgccctgg
      181 tgaaagagcg cctcgaccgc gccatcgaag cgctcgaggt cgccgatgaa ctcggcctgg
      241 tcgaggtagt ccgcgatctc cgccccggcg aggttgcgga acttgtccgc gtgggcgaga
      301 ttgtccaccg cgatcacctc ggtgattccg cgccggttca ggccctcgac gagcttggag
      361 ccgatgaatc cggcggcgcc cgtgactatg cagtacatgg attagcgcgc cccgaagagt
      421 tcctggtgcc ttaccgcggc cgtgccgagc ttgccgacca cgatgccggc ggcgcggttg
      481 gccagacgca ctgccgcttc gagcctggcg cccgccgcca gcattaccgc aagcgtcgcg
      541 atcaccgtat cgcccgcgcc ggtgacgtcg aacacttcgc gcgcctcggc cttgattgtc
      601 gtcgcccggt tccttgcgaa taaggtcatg ccatcctcgc cgcgcgtgag aagcagcgct
      661 tccagcccaa gcttctcgcg cagcgcctgt gcgcggcttt tcagatcctt ctcgtccttc
      721 caggtcccga tcacctcgcg cagctccgcg aggttcggcg taatgatgct cgcgttcctg
      781 tagcgcgaat agtcgtcgcc cttcgggtcg accaggacac gcttccccgc gcggcgcgcg
      841 gcgcggatca tttccacgat atgcgcgagg ccgcccttgc cgtagtcgga gagcagcacc
      901 acgtcgcagt cggcgagcgc gcgctggaat tcctcgagct tggaggcgag cacctcggtc
      961 gagggcggct tctcgaagtc gatgcgcaag agctgctgct tgcggccgat gacgcgcagc
     1021 ttctgcgtcg tgctgagccc ccggtcccgg tgcaggctcg cttcgatgcg ccgcgccttg
     1081 agcagccttt cgagcgcctg ccccggctcg tcgcgtccca cgaccgaaag cagccgcgtg
     1141 cgcgcgccga gctccttgca gttccaggca acgttcgccg cgccgccgag ccgctggtcc
     1201 tcctcgcgga tcaggacgac ggggacgggc gcctcgggcg aaatgcgcga cacatcgccg
     1261 aaccagtagc ggtcgagcat cacgtcgccc gcgaccagga cgcgcgcctt ggccgtatct
     1321 atgttcttct cccgatcggg tagtactcga ggccgagcgc tttcagcgcg gccggatcgt
     1381 acaggttgcg cccgtcgaag atggccgggg tcttgagccg ctcgcggatc gccgcgaaat
     1441 cggggctgcg gaactcctgc cactcggtgg cgatggcgag cacgtcagcc ccctcgagcg
     1501 cagccattgc ggaatccagc accgcgacgc ggttctcgcc tgcgaaaatc ttccgcgctt
     1561 gctcggccgc ggcgggatcg tagccgcgca ccgacgcccc ggcggcgaca agatcggcga
     1621 ccacggtaag gctcggcgcc tcgcgcatgt cgtcggtatt cgccttgaac gcaaggcccc
     1681 aaagcgcaat tgtcctgccg cgcaggtcct tcccgagccg ggccttcact ttctctgcca
     1741 gcacgtgctt ctgcgcctcg ttcaccgctt ccaccgcggc gagcacgcgc agcggcttgc
     1801 ccgcctccgc ggcgctgcgc tgcagtgcct tcacatcctt cggaaagcag gagccgccga
     1861 acccggcgcc ggcgtacagg aactggtacc cgatgcgcgg gtcggagccg acgccccggc
     1921 gcacctcctc gatgtcggcg ccgatcgcct cggccaggtt cgcgagctcg ttcatgaacg
     1981 agatgcgcgt cgcgagcatc gagttcgccg cgtacttggt gagctccgcc gagcgcacgc
     2041 tcatcaccat gatgcgctcg tggttgcgct ggaatggcgc atagagctgg cgcagcagcg
     2101 ccaccgcgcg ctcgtcgtcg gcgccgatga tgacgcggtc cgggcgcatg aagtcctcga
     2161 ccgctgcgcc ttccttgagg aactccgggt tggagaccat cgagaagcgc aatgacaccc
     2221 cgcgcttggc gagcacctcc gcgatcgccg cctgcacctt gtcc
//
LOCUS       DASUNT010000377         4525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_53253, whole genome shotgun sequence.
ACCESSION   DASUNT010000377 DASUNT010000000
VERSION     DASUNT010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53253"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFX94_07645"
     CDS             <1..298
                     /locus_tag="VFX94_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEX5768090.1"
                     /translation="QKYGMLEGRVAQVSADATESPNPNTRSGGLAGRDRPAGALAFRT
                     LVDLAHQHLESDGNRYALAPGMQVLAEIHLGDRTVLEYLLSPVRKAFHEAGRER"
     gene            322..489
                     /locus_tag="VFX94_07650"
     CDS             322..489
                     /locus_tag="VFX94_07650"
                     /inference="COORDINATES: protein motif:HMM:NF023665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HEX5768091.1"
                     /translation="MDYVPPDPTQIEKKPEALDYWARTLETDPEKIRRAVDKVGPVLE
                     KVKQELGIAGV"
     gene            578..982
                     /gene="rpsF"
                     /locus_tag="VFX94_07655"
     CDS             578..982
                     /gene="rpsF"
                     /locus_tag="VFX94_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX5768092.1"
                     /translation="MRHYEIVFIVHPDQSEQVPQMVERYKGLIAQRNGTVHRLEDWGR
                     RQLAYPIAKVHKAHYVLMNIECDNETLKELEHSFKFSDAVLRHLIVAMPKAHTTPSPM
                     MREEKSKSLMERGAGAPGEAAPEPGRAERPAA"
     gene            993..1271
                     /gene="priB"
                     /locus_tag="VFX94_07660"
     CDS             993..1271
                     /gene="priB"
                     /locus_tag="VFX94_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEX5768093.1"
                     /translation="MRYTPAGIAAVEFKLAHQSQQDEAGGKRNVEAEVSAVAFETQAK
                     LLAARPLGSSLKVEGFLGAKSKRSKRLVLHVTDIEFIEGEQHATAPQR"
     gene            1249..1536
                     /gene="rpsR"
                     /locus_tag="VFX94_07665"
     CDS             1249..1536
                     /gene="rpsR"
                     /locus_tag="VFX94_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX5768094.1"
                     /translation="MPPPRRGKGKGKFGKDKKDRGQRALFRRRKFCRFTAEKVEWIDY
                     KDVETLKDFVNETGKIIPARITGTAAGYQRQLSEAIKRARFLALLPYTDLH"
     gene            1538..1987
                     /gene="rplI"
                     /locus_tag="VFX94_07670"
     CDS             1538..1987
                     /gene="rplI"
                     /locus_tag="VFX94_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX5768095.1"
                     /translation="MEVILLEKIANLGELGQVVKVKDGYARTCLIPQGKAKRATEENR
                     KAFEARRAELEQAQAAALAKAQERAGKLDGLTLQITQKAGPDGRLFGSVTNYDIVEAL
                     KKQGHEVERAQIRMPQGPLKQVGDFPIQIGLHTDVAVTIKVSVLGEQ"
     gene            2264..3688
                     /gene="dnaB"
                     /locus_tag="VFX94_07675"
     CDS             2264..3688
                     /gene="dnaB"
                     /locus_tag="VFX94_07675"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX5768096.1"
                     /translation="MPVAHPVARPDAAERELVALKLPPHSVEAEQSLLGALLLDNQAF
                     DRVADLVASQDFYRDDHRRIWRHIARLIEASRPADVVTVSESIEASEDKDKTGGAAYL
                     AALAQNTPSALNIRRYAELVRERAVQRRLAQVATEIAETALAPTGKDVGQLLDEAESK
                     IFQIAESGARKDQGLIGISPVLAKVFERIDHLHSQDNPSDVTGVPSGFVDVDRKTAGL
                     QPGDLIIVAGRPSMGKTAFALNIAEHVALHPSVGLPVAIFSMEMSSSQLAMRMLSSMA
                     RVDQSKLRTGRLDNEEWANLTDAIGKLNEARIHVDETAALNALELRARARRMKREYGK
                     LGLVVVDYLQLMSATSQGENRATEISEISRSLKALAKELDVPVIALSQLSRAVEQRND
                     RRPLMSDLRESGAIEQDADVILFIYREEVYAPEKEEARGRAEVIIGKQRNGPIGTIAL
                     TFMGQFTRFANALAFDGGGQWPNR"
     gene            3673..3918
                     /locus_tag="VFX94_07680"
     CDS             3673..3918
                     /locus_tag="VFX94_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX5768097.1"
                     /translation="MAEPLIAQRSPYAVELAPGDYWWCRCGQSRRQPFCDGSHKSTEF
                     TPVKFSMSEAQKVWLCGCKRSGNKPFCDGSHKKLPES"
     gene            complement(4000..>4525)
                     /locus_tag="VFX94_07685"
     CDS             complement(4000..>4525)
                     /locus_tag="VFX94_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5768098.1"
                     /translation="GVAGTGAGKAVIRHGDIFATDFTQASVITLYLLPALNMKLRPQL
                     LSMRPGTRIVSHSFSMEDWEADEISTLDGRRAYFWIVPANVMGNWTLDAGGQHHDMTL
                     EQTFQKVSGTLALGPIHAGLRDLKLRGTAISFSYVDQNKVRRDFTGRVNGARMEGTFR
                     DEKGAEGKWTATKK"
BASE COUNT          846 a         1478 c         1502 g          699 t
ORIGIN      
        1 ccagaagtac ggcatgctcg agggccgcgt ggcccaggtg agcgccgacg ccaccgagtc
       61 gcccaatccg aacacgcgct cgggcggtct tgcggggcgc gaccgccccg caggagcgct
      121 cgcgttccgg acgctggtcg acctcgcgca ccagcatctc gagagcgacg gcaaccgcta
      181 cgcgctggcg ccgggcatgc aagtgctcgc cgagatccat ctcggcgatc gcaccgtgct
      241 cgagtacctg ctctcgccgg tgcgcaaggc cttccacgag gccgggcgcg aacgttaggc
      301 gcgcgcggca gtcctatggc catggactac gttcccccgg acccgactca aatagagaag
      361 aagcccgagg cgctcgacta ctgggcacgc acgctcgaga ccgaccccga gaagatccgc
      421 cgcgccgtgg acaaggtcgg cccggtgctc gaaaaggtaa agcaggagct cggcatcgcc
      481 ggcgtgtagg gcttgttttt gttgttataa ttcgcgccct tccgggtcgc gctgctacgc
      541 atggcaagcc gaccaaaccc acaggaaagg agcacccatg cggcattacg agatcgtctt
      601 catcgtccat cccgaccaga gcgagcaggt gccgcagatg gtcgagcgct acaagggcct
      661 gatcgcccag cgcaacggca ccgtgcaccg cctcgaggac tggggccgcc ggcagctcgc
      721 ctacccgatc gccaaggtgc acaaggcgca ctacgtgctg atgaacatcg agtgcgacaa
      781 cgagacgctg aaagagctcg agcactcgtt caagttcagc gacgcggtgc tgcgccacct
      841 gatcgtggcg atgcccaagg cgcacaccac gccctcgccg atgatgcgcg aggagaagtc
      901 gaagtcgctg atggagcggg gcgccggcgc ccccggcgag gcggcgcctg aaccgggtcg
      961 agctgagcgg ccggctgctt gagctcggcg cgctgcgcta tacgccggca ggcatcgccg
     1021 cggtcgagtt caagctcgcg caccagtcgc agcaggacga ggcgggcggg aagcgaaacg
     1081 tcgaggccga ggtgagcgcc gtggctttcg aaacgcaggc gaagctgctg gcggcaaggc
     1141 cgctcggcag ctcgctcaaa gtggaaggct ttctcggagc gaagagcaag cgctcgaagc
     1201 gcctcgtgct ccacgtcacc gacattgaat tcatcgaagg agagcaacat gccaccgccc
     1261 cgcagaggta agggcaaagg gaagttcggc aaggacaaga aggatcgtgg ccagcgggcg
     1321 ctgttccgcc ggcgcaagtt ctgccgcttc accgccgaga aagtcgagtg gatcgactac
     1381 aaggacgtcg agacgctcaa ggatttcgtc aacgagacgg gcaagatcat cccggcgcgc
     1441 atcaccggga ccgcggcggg ctaccagcgc cagctgtcgg aggcgatcaa gcgggcgcgc
     1501 ttcctggcgc tgctgcccta cacggacctg cactgacatg gaagtcattc tcttggagaa
     1561 gatcgccaac ctgggcgagc tcggccaggt ggtgaaagtg aaggacggct acgcgcgcac
     1621 ctgcctcatc ccgcagggca aggcgaagcg cgcgaccgag gagaaccgca aggcattcga
     1681 ggcgcgccgc gccgagctcg agcaggcgca ggccgccgcg ctcgccaagg cgcaggagcg
     1741 cgccggcaag ctcgacgggc tgacgctcca gatcacgcag aaggccggcc ccgacgggcg
     1801 gctcttcggt tcggtcacca actacgacat cgtggaggcg ctcaagaagc agggccacga
     1861 ggtcgagcgc gcgcagatcc gcatgccgca ggggccgctc aagcaggtgg gcgatttccc
     1921 gatccagatc ggcctgcaca ccgacgtcgc cgttaccatc aaggtctcgg tactcggcga
     1981 gcagtagccg ctttcagcgg gctgacgaag aaagggctta cgcgcgccct ctctttgctt
     2041 cctctgtcaa gaaaaaaagt tttcggttgt gcaggtatca tgcacacgtt atcgacacgc
     2101 tgcgcacagc taatccacag gcgagagggc gcttcgtcct cggagcggcg gatttttcag
     2161 cagcggcacc cggcatttct ttccgcgagc cgcgcgtttc gacttatcaa atcgaaaacg
     2221 catgcgcgct tcctatgatg cgcgccgggg aggttcaaac acgatgcccg ttgcacatcc
     2281 ggtagcgcgg ccggacgccg ccgagcgcga gctcgttgcg ctgaagctgc cgccgcactc
     2341 ggtcgaggcc gagcagtccc tgctcggggc gctgctgctc gacaaccagg cgttcgaccg
     2401 cgtcgccgac ctcgtcgcgt cgcaggattt ctaccgcgac gatcatcgcc gcatctggcg
     2461 ccacatcgcg cgcctgatcg aggcgagccg gcccgccgac gtggtgacgg tgtcggagtc
     2521 gatcgaggcg agcgaggaca aggacaagac cggcggcgcc gcgtacctcg ccgcgcttgc
     2581 gcagaacacg ccgagcgccc tcaacatccg gcgctacgcg gagctcgtgc gcgagcgcgc
     2641 ggtgcagcgt cggctcgcgc aggttgccac cgaaatcgcc gaaaccgcgc tcgcccccac
     2701 cggcaaggac gtggggcagc tcctcgacga ggccgagtcg aagatcttcc agatcgccga
     2761 gtcgggggcc cgcaaggacc aggggctgat cgggatcagc ccggtgctcg ccaaggtttt
     2821 cgagcgcatc gaccacctcc atagccagga caacccctcc gacgtcaccg gggtgccgtc
     2881 gggattcgtg gatgtcgacc gcaagacggc gggcctgcag cccggggacc tgatcatcgt
     2941 cgccgggcgc ccgtcgatgg gcaagaccgc cttcgcgctc aatatcgccg agcacgtcgc
     3001 gcttcatccg agcgtcggcc tgccggtcgc aatcttcagc atggagatga gctcgagcca
     3061 gctcgccatg cggatgcttt cttccatggc aagggtcgac cagtccaagc ttcgtaccgg
     3121 aaggctcgac aacgaggaat gggcgaacct gaccgacgcg atcgggaagc tgaatgaggc
     3181 gcgcatccac gtggacgaga ccgcggccct caatgcgctc gagctgcgcg cccgggcgcg
     3241 gcgcatgaag cgtgagtacg gcaagctcgg cctggtagtg gtcgactacc tgcagctcat
     3301 gtcggccacc tcccagggag agaaccgcgc gaccgaaatc tcggaaatct cccgctcgct
     3361 caaggcgctt gccaaggagc tcgatgtgcc ggtgatcgcg ttgtcgcagc tttcccgggc
     3421 agtcgagcag cgaaacgacc gccggccgct catgtccgac ctgcgcgagt ccggcgccat
     3481 cgagcaggac gccgacgtca ttctttttat ctaccgcgaa gaggtgtatg ccccggagaa
     3541 ggaagaagcg cggggtaggg ccgaagtcat catcggaaag cagagaaacg ggccgatagg
     3601 taccattgcg ctgacgttca tggggcagtt cacccgcttc gcgaacgccc ttgctttcga
     3661 cggaggcgga caatggccga accgctgatc gcgcaaagat ctccctacgc cgtcgagctc
     3721 gcgcccggag actactggtg gtgccgctgc gggcagtcga ggcgccagcc gttttgcgac
     3781 ggctcgcaca agagcaccga gttcacgccg gtgaagttca gcatgagcga ggcgcagaaa
     3841 gtctggctgt gcggctgcaa gcgcagcggc aacaagccct tctgcgacgg gtcacacaag
     3901 aagctgcctg agtcttagga attccgggac ggagccctga tcacttcgcc tcgctacgaa
     3961 gttagggctc cgtccccgat ttaggcagtc cccgagctgc tacttcttgg tggcggtcca
     4021 cttcccttcc gcgcccttct cgtcgcgaaa ggtgccttcc atcctggcgc cgttgacgcg
     4081 gccggtgaag tcgcggcgca ccttgttctg gtcgacatag gagaagctga tcgccgtgcc
     4141 gcgcagcttc aggtcgcgca gccccgcgtg gatcgggccg agcgcgaggg tgccgctcac
     4201 cttctggaag gtctgctcga gcgtcatgtc gtggtgctga ccgcccgcgt cgagggtcca
     4261 gttccccatc acgttggcag gcacgatcca gaagtaggct cgccggccgt cgagcgtgga
     4321 gatctcgtcg gcttcccagt cctccatgct gaacgagtgc gacacgatgc gcgtgccggg
     4381 ccgcatcgac aggagctgcg gcctgagctt catgttgaga gccggtagga ggtacagcgt
     4441 gatgacgctg gcctgggtga agtcggtggc gaagatgtcg ccgtggcgga tcaccgcctt
     4501 gcccgcgccg gttcccgcga cgccc
//
LOCUS       DASUNT010000378         2890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_842673, whole genome shotgun sequence.
ACCESSION   DASUNT010000378 DASUNT010000000
VERSION     DASUNT010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842673"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="VFX94_07690"
     CDS             complement(<1..1166)
                     /locus_tag="VFX94_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5768099.1"
                     /translation="MAEALVRFLTNQELDGVPLFAGVWAIFGHGNVAGLGPALHAARR
                     KLPTLRAHNEQAMAHAAIAFAKAHARRRMMACTTSIGPGASNMLTAAALAHVNRLPVL
                     LLPGDVFASRRPDPVLQQLENFGDPGASVNDCFRPVSRYFDRITRPEQILVSLPAALR
                     VLLDPADCGPVTLALPQDVQTEVYDYPAEFFRRRVHRIKKPLPDAGEMEAVSKFLRGS
                     RKPLVIAGGGVKYALAEKRLAQFAQTHGVPVAETQAGKGALAWDHPCNVGAIGVTGSR
                     AANALAARADLVVAIGTRLGDFATGSRALFPKAKLVQLNVGAFDAGKHGALPLVADAD
                     AGLEALGRALEGWRAPAVWSAEAARLARRWNSEVERITAPGRGKPTDAQVLRMV"
     gene            1226..2215
                     /gene="iolG"
                     /locus_tag="VFX94_07695"
     CDS             1226..2215
                     /gene="iolG"
                     /locus_tag="VFX94_07695"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEX5768100.1"
                     /translation="MLEFAQFGAGRIGQIHAANIAAMKDVRLRYVVDVNADAAQELAE
                     RHGAKVASERDALADPEVGAVLIASSTDTHARLAIAAARAGKAIFCEKPIDLSLAKVD
                     ACLQEVEKAGVPMLVGFNRRFDPSFSALKKRVDAGEIGAVEQAVISSRDPGPPALSYL
                     RVSGGMFRDMTIHDFDMARWLLGEEPVEVFAMGECLVDRRIGAAGDIDSAMVLLRTAS
                     DRMCHINNSRRAAYGYDQRIEVFGAKGMLRAENIGATTVEHFGPRGTVADRPLDFFLE
                     RYADAYRAEMAAFVSSLRSNTRMPVGAADGRQALVLAEAALQSHRKGKPVKLG"
     gene            2250..>2890
                     /locus_tag="VFX94_07700"
     CDS             2250..>2890
                     /locus_tag="VFX94_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5768101.1"
                     /translation="MHAENLAQRRDARLVYVVDTKPEAARALAEPLGARQASAEEVFS
                     DPKVQAVLIASSTGSHADLSIAAAGAGKAIFCEKPVDLTLPKVDECIRAVRKAKVPMF
                     VGFNRRFDPSFRSLKKRLDAGAIGRLEQVVITNRDPGLPELRFLATSGGLFLDFTIHD
                     FDMARWLLGEEPVEVFAWGVVLVDPRVRTEGKDIDTAMLLLRTASGKMCHINNT"
BASE COUNT          482 a          979 c          982 g          447 t
ORIGIN      
        1 tttaccattc gcaggacttg cgcatcggta ggcttgccgc gcccgggggc ggtgatgcgc
       61 tcgacctcgc tgttccacct gcgcgcaagg cgcgcggctt cagcactcca cactgccgga
      121 gcgcgccagc cctccagcgc gcgtccaagc gcttcgagcc cggcatccgc gtccgccacg
      181 agcggcagcg cgccgtgttt gccggcgtcg aacgcgccga cattgagctg caccagcttc
      241 gctttgggga aaagcgcgcg cgagccggtg gcgaaatcgc cgaggcgcgt gccgatggcg
      301 acgacgaggt cggcccgcgc ggcaagcgcg ttggcagccc gcgagccggt gacaccgatc
      361 gcgccgacgt tgcacgggtg atcccaggca agagctccct tgcccgcctg ggtctcggct
      421 accggcacgc catgcgtctg cgcgaattgg gcgaggcgct tttccgcgag cgcgtacttg
      481 accccgcctc ccgcgatgac caggggcttc ctggaaccac gaaggaattt cgaaaccgcc
      541 tccatctcgc cggcatcggg caacggcttt ttgattcgat gcacccggcg ccggaagaac
      601 tcggcgggat agtcgtacac ctcggtctgc acgtcctgcg gcagcgcgag cgtgactggc
      661 ccgcaatcgg ccggatcgag cagcacccgc agagccgccg gcaggctgac caggatctgc
      721 tcgggacgcg taatgcgatc gaaatagcgc gataccggcc gaaagcaatc gtttaccgag
      781 gcgcccgggt cgccgaaatt ctccagttgc tgcagcaccg ggtcgggccg gcggcttgcg
      841 aatacgtcgc cgggaagaag cagcaccggc agccggttga catgcgcgag cgccgcggcg
      901 gtcagcatgt tgctcgcccc ggggccgatc gaggtggtgc aggccatcat gcgacggcgc
      961 gcgtgcgcct tggcgaacgc gatcgcggcg tgcgccatcg cctgctcgtt atgcgcgcgc
     1021 agggtcggca gcttgcgtcg cgcagcgtgc aaggcgggcc cgagaccggc gacgttgccg
     1081 tgcccgaaga tcgcccacac tccggcgaaa agaggaacgc cgtcaagctc ctggttcgtc
     1141 aggaaacgaa ccagcgcctc cgccatggtg atgcgaaccg ctgccacggg cctcccctct
     1201 ctggatttat catacacaaa caagaatgct ggaattcgcc cagttcgggg ccggacgcat
     1261 cggccagatc cacgccgcca acatcgctgc catgaaggac gtgcggctgc gctacgtcgt
     1321 ggacgtgaac gccgatgccg cgcaagagct tgccgagcgc catggcgcga aggtcgcgtc
     1381 cgagcgcgat gcgcttgccg acccggaagt cggcgccgtg ctcatcgcct cgtcgaccga
     1441 cacccatgcg cgcctcgcga tcgccgccgc ccgcgccggc aaggcgatct tctgcgagaa
     1501 gccgatcgac ctttcgctcg ccaaggtcga tgcctgcctg caggaagtgg aaaaagcggg
     1561 cgtgccgatg ctcgtcggct tcaaccgccg cttcgacccg agcttcagcg cgctgaaaaa
     1621 gcgcgtcgac gccggcgaga tcggcgcggt cgagcaggcg gtgatctcga gccgcgatcc
     1681 agggccgccg gcgctttcct acctcagggt gtcgggcggc atgttccgcg acatgacgat
     1741 ccacgatttc gacatggcgc gctggctgct cggcgaggag ccggtggagg tgttcgcgat
     1801 gggcgagtgc ctggtcgacc gcaggatcgg cgcggccggc gacatcgact ccgcgatggt
     1861 gctgctgcgc accgcttcgg accgcatgtg ccacatcaac aactcgcgcc gggccgccta
     1921 cggctacgac cagcgcatcg aggtgttcgg ggcaaagggc atgttgcgcg ccgagaatat
     1981 cggcgcgacc accgtggagc acttcgggcc gcgcggcacg gtcgcggacc ggccgctcga
     2041 cttcttcctc gagcgctacg cggacgccta tcgcgccgaa atggcggcat tcgtttcttc
     2101 attgagaagc aacacccgga tgccggtcgg cgccgccgac ggccgccagg cgctggtgct
     2161 ggccgaggcg gcgctccagt cgcacaggaa gggcaagccg gtcaagcttg gttaatttcg
     2221 cgctcttcgg cgccggacgg atcgggcgca tgcacgccga aaacctcgcg cagcggcgcg
     2281 acgcgaggct cgtctacgtc gtcgacacga agcccgaggc ggcgcgcgcg ctcgccgagc
     2341 cgctcggcgc gaggcaggca agcgcggaag aggtcttctc ggatccgaaa gtccaggcgg
     2401 tcctgatcgc ctcgtccacc ggcagccacg ccgacttgtc gatcgccgcg gcgggcgccg
     2461 gcaaggcgat cttctgcgag aagccggtgg atctcacact accgaaggtc gacgagtgca
     2521 tccgggcagt acgcaaagcg aaagtgccga tgttcgtggg gttcaaccgg cgcttcgatc
     2581 cgagcttccg cagcctgaag aagcgcctcg acgccggcgc gatcggcagg ctcgagcagg
     2641 tggtcatcac gaaccgcgat ccgggcctgc ccgagctgcg cttcctcgcg acctcgggcg
     2701 gcctttttct cgatttcacg atccacgact tcgacatggc gcgctggctc ctcggcgagg
     2761 agccggtcga ggtcttcgcg tggggcgtgg tgctcgtcga tccgcgcgtg cgaaccgagg
     2821 gcaaggacat cgataccgcg atgctgctgc tgcgcacggc atcgggaaag atgtgtcaca
     2881 tcaacaatac
//
LOCUS       DASUNT010000379         3490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1193519, whole genome shotgun sequence.
ACCESSION   DASUNT010000379 DASUNT010000000
VERSION     DASUNT010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193519"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1863)
                     /locus_tag="VFX94_07705"
     CDS             complement(139..1863)
                     /locus_tag="VFX94_07705"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5768102.1"
                     /translation="MSSRAYRITPTDSTTFYPRLIGRHGAVAANSYLSVNAGVDILKA
                     GGNAIDAACAATLVEGLVNPQMHTLGGECPILIRAAGSHVVCLNGNTAAPGAATPEAF
                     RKRGLSDVPEEGILAAGVPAAFSAVMTALTRWGTMSLREVSAHARELAARGFPVSAGL
                     RHQHKYGVLSLQERFRNEWPASGHLYLRNGRVPEVGEVMENRALARVLEHLAGAKDPH
                     AEFYRGDVAAEIAKFSKEKDGLLARADLERFETRIEEPASLCFGDIELFKSGFWSQGP
                     AELQTVALMWPYLKEVKPGSPDHCHLLIEAMKLAFADREQYYGDTLVPGEVLLSCEYT
                     GQRAGLIDMQKSNRELRPGDPRRNAALLPEDERFTPKDWGGGTVHVDAVDAKGNMASF
                     TPSGGWLMSSEVIPALGFPLSVRMMTFYLEPAHHPNVVAPHKRPRTTLTPSLAFKGGK
                     PWMTFGSMGGDNQGQWLLQFFVYRAAFGMTLQDAIEAPRLSTEHFPGFFAPHRGVPNR
                     VRIEPRFGAKVIDELRRRGHDVELAPDWSEGFVSGAQLDEASGLLEAGCDPRGSKSEC
                     FPAFAYCW"
     gene            1885..2193
                     /locus_tag="VFX94_07710"
     CDS             1885..2193
                     /locus_tag="VFX94_07710"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ family domain-containing protein"
                     /protein_id="HEX5768103.1"
                     /translation="MLGFLAEQKILEAISRGELDGLPGEGQPLELDDDKLVPEDLRLA
                     YRILKNAGFVPPEVETLNEMAALERLVSSDSKAARKLALLKTCIEARYYEKAIAKLNR
                     "
     gene            complement(2197..3459)
                     /locus_tag="VFX94_07715"
     CDS             complement(2197..3459)
                     /locus_tag="VFX94_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5768104.1"
                     /translation="MSRASRPSFVFILADDLGYADLGCYGGRGNCSPNLDRIAAEGLR
                     FTDGYANSPVCSPTRFALVTGRWQYRFRGGADEPIASRLRGSDVLGLPPEHPTLASLL
                     RDAGYATALVGKWHLGFAPHFGPLKSGYQEFFGVMSGGVDYFTHCDSAGVHDLWQGEK
                     EVHRNGYLTDLLTGEAIAFVRKAKRKPFLLSLHYTAPHWPWEARADAEESKTIAGISH
                     VDGGSVATYLEMIRAMDEGIGRLARELDDNTAVVFTSDNGGERFSDSWPLVGGKMDLL
                     EGGIRVPYIVRWPKGGVIRQLAITMDWVATFLDAADVAPHPDFPLDGVSLLGEHSRRE
                     LFWRMKFRSQKAMRSGSWKWLSIERDEFLFNLAQEQRERANLAKRQPGMLSELRRRYF
                     DWEASMPPIPQDARVSLVYGPSDIPKPS"
BASE COUNT          609 a         1151 c         1135 g          595 t
ORIGIN      
        1 tggaagtcgg gtgcgatgtc gccaatacga agtgccatgg atctcccttt caaaaagcac
       61 tcagtgtccg gcaagcacct gtccgaaaac cgcaagcggt gtgaaaaaag cgcatcaaaa
      121 gctgcgcact cgcctgcttt accagcagta agcgaaggcg ggaaaacact ccgatttcga
      181 gccgcgcggg tcgcacccgg cttccagcag gccgctcgcc tcgtcgagct gcgcgccgga
      241 gacgaagccc tcgctccagt cgggcgcgag ctcgacgtcg tggccgcggc ggcgcagctc
      301 gtcgatcacc ttcgccccga agcgcggctc gatgcgcacg cggttcggca cgccgcggtg
      361 cggggcgaag aagccgggga aatgctcggt ggaaagccgc ggcgcctcga tcgcgtcctg
      421 cagcgtcatg ccgaaagccg cacggtagac gaagaactgc agcagccatt gcccctggtt
      481 gtccccgccc atgctgccaa acgtcatcca tggcttgccg cccttgaacg caagcgacgg
      541 cgtgagggtg gtgcgcgggc gcttatgggg cgccacgacg ttcggatggt gcgcgggctc
      601 gagatagaac gtcatcatgc gcaccgagag cggaaagccg agcgcgggaa tcacctcgga
      661 ggacatgagc cagccgccgc tcggcgtgaa tgaggccatg ttgcctttgg cgtcgacggc
      721 atcgacatgg accgtgccgc cgccccagtc cttcggcgtg aagcgctcgt cctcgggcag
      781 cagcgcggca ttacgccgcg ggtctcccgg gcgaagctca cggttgcttt tctgcatgtc
      841 gatcaaccct gctcgttgcc cggtgtattc gcaggagagc aggacctcgc cgggcacgag
      901 cgtgtcgccg tagtactgct cgcggtcggc gaaggcgagt ttcatcgcct cgatcaggag
      961 atggcagtgg tcggggctcc cgggcttcac ctccttgagg tagggccaca tgagagcgac
     1021 cgtctgcaat tccgccggcc cctgcgacca gaagcccgac ttgaacagct cgatgtcgcc
     1081 aaagcacagg cttgccggtt cttcgatccg tgtttcgaac cgctccagat ctgcccgcgc
     1141 cagcagtccg tccttttctt tggaaaactt cgcgatctcc gcggcaacgt cgccgcgata
     1201 gaactcggcg tgcgggtctt tcgcaccggc caggtgctca agcacgcggg cgagcgcccg
     1261 gttctccatt acctcgccca cttccggaac ccgcccgttc ctgaggtaca ggtgcccgct
     1321 tgccggccat tcgttgcgaa atctttcctg caacgaaagc accccgtatt tgtgctggtg
     1381 gcgcaggccg gccgacacgg gaaagcccct ggcggcgagc tcgcgcgcgt gcgcggacac
     1441 ctcgcgcagg ctcatcgtcc cccagcgtgt aagcgccgtc attacggccg agaacgctgc
     1501 tggcacgccg gcagcgagga tcccctcttc ggggacatcc gataagccgc gtttcctgaa
     1561 cgcctcgggc gttgccgcac cgggcgcggc ggtgttgccg ttgaggcaca cgacgtgcga
     1621 gcccgccgcc cggataagaa tcgggcattc gccgccgagc gtgtgcatct gcggattgac
     1681 cagcccttcg accagggtcg cggcgcaggc ggcgtcgatc gcgttaccac cggctttcag
     1741 gatgtccacg ccggcgttga ccgaaaggta cgagttcgcc gcgacggcgc cgtgccggcc
     1801 gatcaagcgc ggatagaagg tggtcgaatc ggtgggtgtt atccggtatg cgcggctgct
     1861 cacgctacta gaataccttt ccggatgctg ggtttcctgg cggagcagaa gattctggaa
     1921 gctatttcgc gcggcgagct cgacggcctg cccggcgagg gacagccgct cgagctcgac
     1981 gacgacaagc tcgttcccga ggacctgcgc cttgcttacc gcatcctcaa gaacgccggg
     2041 ttcgtgcccc ccgaagtcga gacgctcaac gagatggccg cgctcgagcg cctggtcagc
     2101 agcgactcca aggcggcgcg caagctcgcg ctcctcaaga cctgcatcga ggcgcgctac
     2161 tacgaaaaag cgatcgccaa gctgaaccgc taggactcag gacggtttgg ggatgtcgga
     2221 cgggccgtag accagcgaaa cccgcgcgtc ctgcggaatg ggcggcatgg acgcttccca
     2281 gtcgaagtag cgtcttctca gctcgctcaa catgccgggc tggcgctttg ccaggttggc
     2341 gcgctcgcgc tgctcttgcg ccaggttgaa caggaactcg tcgcgttcga tcgagagcca
     2401 cttccagctt ccgcttcgca tcgccttctg gctgcggaat ttcatccgcc agaaaagctc
     2461 gcgccgcgaa tgctccccga ggaggcttac gccgtcgagg ggaaaatcgg gatgcggggc
     2521 gacatccgcc gcgtcgagga aagtcgccac ccaatccatc gtgatggcga gctgccggat
     2581 cacgccgcct ttcggccagc gcacgatgta cggcacgcgg atgccgccct ccaggaggtc
     2641 catcttgcct ccgacgagcg gccagctgtc ggagaagcgc tccccgccgt tgtcgctggt
     2701 gaataccacg gcggtgttat cgtcgagctc gcgcgcgagg cgaccgatgc cctcgtccat
     2761 ggcgcggatc atctcgaggt acgtcgctac cgagccgccg tcgacatggc tgatgcccgc
     2821 aatcgttttc gactcctcgg cgtcggcccg cgcttcccac ggccagtgag gcgcggtgta
     2881 gtgcagcgag agcaggaaag gctttcgctt tgcctttcgt acgaaggcga tcgcctctcc
     2941 ggtgagaagg tcggtgaggt agccgttgcg gtgcacttcc ttctcgccct gccaaaggtc
     3001 gtgcacgccc gccgagtcgc agtgcgtgaa gtaatccacc ccgccgctca tcacgccgaa
     3061 gaactcctgg tagccgcttt tcagcgggcc gaagtgcggc gcaaatccga ggtgccactt
     3121 gccgacgagc gcggtcgcgt agcccgcgtc gcgcagcagg gaggcgagcg tcggatgttc
     3181 agggggtaat cccaagacat cacttccacg caggcggctc gcaatcggct cgtcggcgcc
     3241 gccgcggaag cggtattgcc accgaccggt gacgagcgcg aagcgcgtcg gcgagcagac
     3301 cggcgaattg gcatagccgt cagtgaagcg cagcccctcg gcggcgatgc gatccagatt
     3361 cggagagcag ttgccgcgtc caccatagca accgaggtcg gcatagccga gatcgtcggc
     3421 gaggatgaag acgaagctcg gcctactcgc ccttgacacc ggtctcgcgg atcacgcggc
     3481 cccaggcggc
//
LOCUS       DASUNT010000380         2725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_317864, whole genome shotgun sequence.
ACCESSION   DASUNT010000380 DASUNT010000000
VERSION     DASUNT010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317864"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1077
                     /locus_tag="VFX94_07720"
     CDS             211..1077
                     /locus_tag="VFX94_07720"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX5768105.1"
                     /translation="MEEQLKLMDQVAAAAIDMAIRFGPKLLVALLILAVGFFAGRWAG
                     RATRRGLAKFHLEPPVLALIERAAHIVVLGLFVIMALQNLGVELLPLIAGLGIAGAGV
                     ALAMQGVLGNAVAGLTIIFTRPFRVGDYISIAKEEGEVLEIKLFNTTLGHADLSRVVI
                     PNRKIVGEILHNYGKIRQVPIEVGVAYDTDVAVALKAAEDVLRANRRVLKDPAPLVRV
                     LKFADSKLVILAAPWVSVPDYGPAGAELYPAILEEFRRRGIVIPFPQRDIRVVSSTAY
                     VGKKEGTYVGSA"
     gene            complement(1059..1442)
                     /locus_tag="VFX94_07725"
     CDS             complement(1059..1442)
                     /locus_tag="VFX94_07725"
                     /inference="COORDINATES: protein motif:HMM:NF040052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6306 domain-containing protein"
                     /protein_id="HEX5768106.1"
                     /translation="MLEAERAGAKALVVYMDDFPRNSEAWKILRQIQADEAHNCALIG
                     KLLEKSGTPYSHATGKVYDRAVAVKGPRARLEFLVRGLKWAVKRFEQALPQLDPSARD
                     VFIKMRDSHLRTIAACERLIQALPT"
     gene            complement(1456..>2725)
                     /gene="boxB"
                     /locus_tag="VFX94_07730"
     CDS             complement(1456..>2725)
                     /gene="boxB"
                     /locus_tag="VFX94_07730"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEX5768107.1"
                     /translation="DVYLRTAISVDAQGWAHYGAVKMPEYRWGIFLADPVPERKIGFG
                     DQMGEPAWQQVPGEYRSTLRRLIVTQGDTEPASVEQQRLLGHTCPSLYDLRNLFQVNV
                     EEGRHLWAMVYLLHAYFGRDGREEAEELLARHSGDADKPRILTTFNEPISDWLSFYCF
                     TYFTDRDGKYQLKSLAESAFDPLSRTCRFMLTEEAHHMFVGETGIGRVVKRTLEVMKE
                     LGTEDPDRIRAAGAVDLPTMQKYLNFWCSSSLDLFGSEISSNSAANFANGLKGRPDES
                     QYEDHVLRSSTLRLLTPEGEQEVAMLNALNEVMRESYLKDCEIGLKRWNRQIERAGFP
                     LRLTLPSVRFRRSIGPWAGQPVTPRGETTGRESFEKSLYDWIPSQSDRAFVHSLMHKV
                     AAPGKMAAWIAPPERGINNLPVDYEYVKLH"
BASE COUNT          449 a          886 c          907 g          483 t
ORIGIN      
        1 tctccgccag gaaaacccga tcgggcgcgc ggtcagacca ccggaggagc cattcggtga
       61 cacagcgcgc gtaggagccg agcttctgcg gcgagcgcag caccatgctg ccgtcggcgc
      121 gtttcaccac ctcgactttc ggcggcgcga accttagatt gcgctccatg ccaggttgga
      181 cattctaaaa gaagccggaa aggagctccg atggaagagc agcttaagct gatggaccag
      241 gtcgccgccg cggcgatcga catggcgata cgcttcgggc cgaaactgct ggtcgcgctg
      301 ctgatcctgg cggtgggctt ttttgccggg cgctgggccg gccgcgcgac gcggcgcggg
      361 ctcgccaagt tccacctcga gccgccggtg ctcgcgttga tcgaacgagc cgcgcacatc
      421 gtggtgctcg gcctcttcgt catcatggcg ctgcagaacc tcggcgtgga gctgctgccg
      481 ctgattgccg gcctgggcat cgccggcgcg ggagtcgccc tcgccatgca aggggtgctc
      541 ggcaatgcgg tggccgggct gacgatcatc ttcacgcgcc ccttccgggt cggcgactac
      601 atctcgatcg cgaaggagga gggcgaggtg ctggagatca agctcttcaa caccacgctg
      661 gggcacgcgg acctttcgcg cgtggtgatc ccgaaccgca agatcgtggg ggagatcctg
      721 cacaactacg gcaagatccg gcaggttccg atcgaggtcg gggtggccta cgacaccgat
      781 gtcgcggtcg cgctgaaggc ggcggaggac gtgctgcgcg ccaaccggcg cgtgctcaag
      841 gacccggctc ccctcgtccg ggtgctcaag tttgccgact cgaagctggt catcctggca
      901 gcgccctggg tcagcgtgcc cgactacggt ccggccggcg ccgagctgta cccggcgatc
      961 ctcgaagagt tccgccgccg cggcatcgtc atcccgtttc cccagcgcga catccgggtc
     1021 gtgtcgagca cggcctatgt cggcaagaag gagggaacct acgtcggcag cgcctgaatc
     1081 agccgctcgc aggctgcgat ggtgcgcagg tgcgagtcgc gcatcttgat gaagacgtca
     1141 cgcgctgacg gatcgagctg cggcagcgcc tgctcgaacc tcttcaccgc ccacttcaat
     1201 ccgcgcacga ggaactcgag gcgcgccctc ggccctttca ccgccaccgc cctgtcgtag
     1261 accttgccgg tggcatggct gtagggcgtg ccggatttct cgagcagctt gccgatgagc
     1321 gcgcagttgt gcgcttcgtc ggcctggatc tggcgcagga tcttccacgc ctcggagttg
     1381 cgcggaaagt cgtccatgta gacgacgagc gccttcgcgc ccgcgcgctc ggcctcgagc
     1441 atctcgttga agaagtcagt ggagcttgac gtattcgtaa tccaccggga ggttgttgat
     1501 gccgcgctcg ggcggcgcga tccacgccgc catcttgccc ggcgccgcga ctttgtgcat
     1561 cagcgagtgc acgaacgccc tgtcggactg cgacggaatc cagtcgtaca gggacttttc
     1621 aaaggattcg cgtcccgtgg tttctccccg gggcgtcacc ggctggccgg cccaggggcc
     1681 gatcgagcgg cggaagcgca ccgagggcag ggtaagccgc aacgggaagc ccgctcgctc
     1741 gatctggcgg ttccagcgct tgagcccgat ctcgcaatcc ttgagatagg actcgcgcat
     1801 cacctcgttc agcgcgttca gcatcgcaac ttcctgttct ccttccgggg taagaagcct
     1861 caaggtcgaa gaacgaagga catgatcctc gtactgtgat tcgtcgggcc tgcccttgag
     1921 tccgttggcg aagttggccg ccgagttgga cgaaatctcc gagccgaaga ggtcgagcga
     1981 gctcgagcac cagaagttca ggtacttctg catcgtcggc aggtccaccg cgccggcggc
     2041 gcgtatcctg tccggatcct cggtgccgag ctccttcatg acttcgagcg tgcgcttcac
     2101 cacgcgaccg atgccggtct cgcctacgaa catgtggtgc gcttcctcgg tgagcatgaa
     2161 gcggcaggtg cgcgagagcg gatcgaaagc gctctcggcg aggctcttca gctggtactt
     2221 gccgtcgcgg tcggtgaaat acgtaaagca gtagaacgac agccagtccg agatcggctc
     2281 gttgaacgtg gtcaggatgc gcggcttgtc ggcatcgccg gagtgacgcg caagcagctc
     2341 ctcggcctcc tcgcgcccgt cgcgcccgaa gtacgcgtga agcaggtaca ccatcgccca
     2401 gaggtggcgg ccttcctcga cgttcacctg gaagaggttc ctcaggtcgt acagcgacgg
     2461 gcaggtgtgc ccgagcaggc gctgctgctc gaccgaggcg ggctcggtgt cgccctgcgt
     2521 gacgatcagg cgccgcagcg tcgagcgata ctcgcccggg acctgctgcc acgcgggctc
     2581 gcccatctgg tcgccgaagc cgatcttgcg ctccggcacc gggtcggcga ggaagatacc
     2641 ccagcggtac tccggcatct tgaccgcgcc gtagtgcgcc cagccctggg catcgaccga
     2701 gatcgcggtg cgcaggtata catcg
//
LOCUS       DASUNT010000381         3166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1017841, whole genome shotgun sequence.
ACCESSION   DASUNT010000381 DASUNT010000000
VERSION     DASUNT010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017841"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VFX94_07735"
     CDS             <1..785
                     /locus_tag="VFX94_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HEX5768108.1"
                     /translation="KKVRLYAGEDVEGVSQSQSEVEKMRAKNPDEGLAGVSPRFVINA
                     LSNAIIQSQAKSLTTMNVLIALKEAIDSDVRIDGKKKRKWIDFLVTVRKDFYNRWVKE
                     DVHKALFVSFEDEAQQLLEKYLDEVEATLDNRSIKDPITNEERKPDERFLRSVEEKIK
                     ISESGKLSFRQEVVRKAMGAYKRGEKFTLDNHAPLHDAVEQYLFEQRRDVLRLVSSAS
                     RPDEETRQKISVVEKRLVDEYGYDSHSAREALNYVTTLLAQE"
     gene            795..1940
                     /locus_tag="VFX94_07740"
     CDS             795..1940
                     /locus_tag="VFX94_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HEX5768109.1"
                     /translation="MIRIAAETPWYELFSRGARDWLRHNQKVRAAVKESLPELLAGAD
                     LISNPGNRTVAVPVKFLEHARFRLLDQDTQTGAGQGKGAPGDVLRPAREEGDQRGQAG
                     TGDGEIRFVVELKVDEIVDWLWEELKLPELKPKRSAALDEPDYVREGWDKRGARSRLD
                     RRRTLKEAVKRHAVQEDGLPIINDDLRFRQLHRRSLPATNAAVVFALDVSGSMDEAQR
                     RLAKQFFFFALQGIRRQYTKVETLFLAHAAQAWEFEESQFFQASSSGGTVSSSVARLA
                     LETLQARYDPSRYNAYFFYASDGENAAEDREAARAALGELAPLLNYAGYVETGGVATF
                     RPRETQLAELFNELKTKGHPVGVSHLGSQDDIWRAIREFFTEQAADD"
     gene            1933..>3166
                     /locus_tag="VFX94_07745"
     CDS             1933..>3166
                     /locus_tag="VFX94_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEX5768110.1"
                     /translation="MTELDEYAARIESLAKRLGLDYYPVDFELAPQSLMTEIAVYGLP
                     VRMPHWSFGVRYIHQLVRQSMGHSKIFEVMFPGDPCRAFLMDSNSLAENALVTAHVIG
                     HADFAKNNQLFARFHEMAGGNIVEHAAAHAQRIQQAIELAGLERVESVLDAALALESH
                     LDVGGELHRARYPEMVAEKPAAKPSPFHKRFQALPGASEPEPPPAVPVRTRVPPHPEY
                     DLLWFIANYAPDLEQWERDIFLAVRAESLYFYPVFACQIMNEGWASYWHARLLREADF
                     LPQPTYVAAIKAHSDVVRPFASGEETALGINPYHLGFTLWETIVERHGFERAREVMRE
                     EDDFGFIRNWLDRELAEKSGLFVFETHPGDEIRVANRDVDAVREAILAPKFNFGAPRV
                     AAVEIKADGGLVLAHEHGS"
BASE COUNT          556 a         1087 c         1059 g          464 t
ORIGIN      
        1 tgaaaaaggt gcgcctgtac gccggcgagg acgtcgaagg cgtgtcgcag tcgcagtccg
       61 aagtggagaa gatgcgcgcc aagaaccccg acgagggcct cgccggggtg tcgccgcgct
      121 tcgtgatcaa cgcgctctcg aacgcgatca tccagtcgca ggcgaagagc ctcaccacca
      181 tgaacgtgct gatcgcgctc aaggaggcga tcgactcgga cgtgcgcatc gacggcaaga
      241 agaagcgcaa gtggatcgac ttcctcgtca cggtgcgcaa ggacttctac aaccgctggg
      301 tcaaggagga cgtgcacaag gcgctgttcg tgtcgttcga ggacgaggcg cagcagctgc
      361 tcgagaagta cctcgacgag gtcgaggcca ccctggacaa ccgcagcatc aaggacccga
      421 tcaccaacga ggagcgcaag cccgacgagc gcttcctgcg ctcggtggag gagaagatca
      481 agatctccga gtcgggcaag ctttccttcc gccaggaagt ggtgcgcaaa gcgatgggcg
      541 cctacaagcg cggcgagaag ttcaccctcg acaaccacgc gccgctgcac gacgcggtgg
      601 agcagtatct cttcgagcag cgccgcgacg tgctgcgcct ggtgtcctcg gcgtcgcgcc
      661 ccgacgagga gacgcggcag aagatctcgg tggtcgagaa gcgcctggtc gatgagtacg
      721 gctacgactc gcacagcgcc cgcgaggcgc tcaactacgt gactacgctg ctcgcgcagg
      781 aatgagctag ctccatgatc cgcatcgcgg ccgaaacccc ttggtacgag ctcttctcgc
      841 gaggtgccag ggactggctg cggcacaacc agaaggtgcg cgccgcagtg aaggagagcc
      901 tgcccgagct cctcgccggc gccgacttga tcagcaaccc cggcaaccgc acggtggcgg
      961 tgccggtaaa gttcctggag cacgcgcgct ttcgcctgct cgaccaggac acgcagactg
     1021 gcgccggcca gggcaagggg gcgccgggcg acgtgctgcg cccggcacgc gaggaaggcg
     1081 accagcgcgg ccaggcgggc accggcgacg gcgagatccg cttcgtggtg gagctcaaag
     1141 tcgacgagat cgtcgactgg ctgtgggagg agctgaagct tcccgagctc aagcccaagc
     1201 gctccgcggc gctggacgag cccgactatg tgcgcgaagg ctgggacaag cgcggggcgc
     1261 gctcgcggct ggaccgccgg cgcaccctga aggaggcggt caagcgccac gcggtgcagg
     1321 aggacgggct gccgatcatc aacgacgacc tgcgcttccg ccagctgcac cgccgctcgc
     1381 tcccggcgac caacgccgcg gtggtgttcg cgctcgacgt ctcgggcagc atggacgagg
     1441 cgcagcgccg cctcgctaag cagttcttct tcttcgcgct gcagggcatc cggcgccagt
     1501 acaccaaagt ggagacgctg ttcctcgccc acgcggccca ggcctgggaa ttcgaagaga
     1561 gccagttttt ccaggcctcc tcgagcggcg gcacggtgag ctcgagcgtc gcccgcctcg
     1621 ccctggaaac gctgcaggcg cggtacgacc cgagccgcta caacgcctac ttcttctatg
     1681 cctccgacgg tgagaatgcc gccgaggacc gggaggcggc gcgcgcggcg ctcggcgagc
     1741 tcgcccccct gctcaactac gcgggctacg tcgagacggg cggcgtggcg accttccgcc
     1801 cgcgcgagac gcagctcgct gagctcttca acgagctgaa gaccaagggc cacccggtgg
     1861 gcgtcagcca cctcggctcg caggacgaca tctggcgcgc aatcagggag ttcttcaccg
     1921 agcaagcggc ggatgactga gctggacgaa tacgccgcgc gcatcgagtc gctcgcgaaa
     1981 cgccttgggc tcgactacta cccggtcgac ttcgagctcg cgccgcagag cctgatgacc
     2041 gagattgcgg tctatgggct gccggtgcgc atgccgcact ggtccttcgg cgtgcgctac
     2101 atccaccagc tcgtgcgcca gagcatgggg cattcgaaga tcttcgaggt gatgttcccc
     2161 ggcgacccgt gccgcgcgtt cctgatggac tcgaactcgc tcgccgagaa cgcgctggtc
     2221 accgcgcacg tgatcggcca cgccgacttc gccaagaaca accagctctt cgcccgtttt
     2281 cacgagatgg cgggcggcaa catcgtcgag cacgccgccg cgcacgcgca gcgcatccag
     2341 caggcgatcg agctggcggg cctggagcgg gtcgaatcgg tgctcgacgc cgccctcgcg
     2401 ctcgagtcgc acctcgacgt cgggggcgag ctgcaccgcg cgcgttatcc ggagatggtg
     2461 gccgagaagc ctgccgccaa gcccagtcct ttccacaagc gcttccaggc gctgcccggc
     2521 gcgtccgagc ctgagccgcc gcctgcggtg ccggtccgca cccgcgtgcc gccgcacccg
     2581 gaatacgacc tgctctggtt catcgccaac tacgcccccg atctcgagca gtgggagcgc
     2641 gacatcttcc tcgcggtgcg cgccgagtcg ctctacttct acccggtatt cgcctgccag
     2701 atcatgaacg aaggctgggc gtcgtactgg catgcgcgcc tgctgcgcga ggccgacttc
     2761 ctgccgcagc ctacctacgt cgccgcgatc aaggcgcact ccgacgtggt gcggccgttc
     2821 gcctctggcg aagagaccgc gctcggcatc aacccctacc acctcggctt cacgctgtgg
     2881 gagacgatcg tcgagcgcca cggcttcgag cgcgcgcgcg aggtgatgcg cgaggaggac
     2941 gacttcggct tcatccgcaa ctggctcgac cgcgagctgg ccgagaagtc cggcctgttc
     3001 gtcttcgaga cccatcccgg cgacgaaatc cgcgtggcca accgcgacgt cgacgcggtg
     3061 cgcgaggcga tcctcgcgcc caagttcaac ttcggcgccc cgcgcgtcgc cgcggtggag
     3121 atcaaggccg acggcggcct ggtgctcgcg cacgagcacg gcagcg
//
LOCUS       DASUNT010000382         3170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_930136, whole genome shotgun sequence.
ACCESSION   DASUNT010000382 DASUNT010000000
VERSION     DASUNT010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930136"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFX94_07750"
     CDS             complement(<1..190)
                     /locus_tag="VFX94_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX5768111.1"
                     /translation="MDVRAAVAHKAGAPLTIETVQLQGPKAGEVLVEIKATGICHTDE
                     FTRSGADPEGLFPTILGHE"
     gene            complement(219..2027)
                     /locus_tag="VFX94_07755"
     CDS             complement(219..2027)
                     /locus_tag="VFX94_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEX5768112.1"
                     /translation="MRRSWQRCLDEYGLDPESSGQPALVSHQELMVRKEQSLELVAFA
                     EIEMAHLQHQLAGSGHSIILTDRDGVVLSYYGDPSFKHAASRAGLVPGGVWSERHGGT
                     NGMGTCLAERAALIVHREQHFLARNTGLTCCAAPIFDPGGELYAVLDASGESDRAQEH
                     TLVLVNLSAQAIEKRLFLHRFRDAFVVRFHSRPDLLGTSSEGMIAFSPAGTIAALDRN
                     ALFQLGCKQAAHLMSGAPLERVFNISLPALLGRSRRKLLHPLPIYEMRHGGRFFALAQ
                     EPDPAQRSSSAGQRASPMPEAASSSALDELGLGDPVMARNIRAAKRVASRAVPTLIVG
                     ETGTGKEVFARALHAASERATRIFVALNCAALPEASLQSALAQARGGTLFLDEIGELP
                     GALQARVLHLLERGEAVLRAGEAPTSIDVRVVAASACSLEDKIRRGQFREDLFYRLQG
                     LVVTLPRLHERADKRALIRHLFAQEAADAHCVSLSERLVDALCAHRWPGNLRQLRNAL
                     RAMIARRSSDRLDLADLPSDYSLGAHAGAAQAGADAADSLNALARAEREALLRELERE
                     RGNISHVARNLGVSRNAVYRKMHRLRIAWPAMEPRH"
     gene            complement(2146..3108)
                     /locus_tag="VFX94_07760"
     CDS             complement(2146..3108)
                     /locus_tag="VFX94_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5768113.1"
                     /translation="MLARALAALAAACLAASLGAAAPFPEPVAVGNGVHVFFGAQEEA
                     SRANWGHIANQGFIIAPRGVIVIDSGLSAAFAEHMLRAIRARTAQPLAILILTWPMDE
                     AIFGAAVFQDQGVPVYVHEAASRLIAERCQRCLESRTAALGADIMAGTRVPRPERLFN
                     GSQLLSVAGRRLELVDESGAAAPGSIAVWDRESGVLFAGGLASFGRVPETRDGNLAEW
                     IRALQRLSRIPARRVIPAHGWAGSPADLKAVAAYLGGLERHTQEAYAAGASLLEAPRN
                     VAVHEYRHWALYETIHPRNVHHAYVALERRELQNELPASRASPP"
BASE COUNT          483 a         1077 c         1120 g          490 t
ORIGIN      
        1 cctcgtggcc caggatggtc gggaacaggc cctccgggtc ggcgcccgag cgggtgaact
       61 cgtcggtatg gcagatgccg gtggccttga tttccaccag cacctcgccc gccttcgggc
      121 cctgcaactg caccgtttcg atggtcaagg gcgcgcccgc cttgtgcgcc accgctgcgc
      181 gaacatccat atgtcacccc ttcgaagagt cgatcgagtt agtgccgcgg ctccatggcc
      241 ggccacgcga tgcgaagccg gtgcatcttg cggtacacgg cattgcggct gacgccgaga
      301 ttgcgcgcga cgtggctgat attgccgcgc tcgcgctcga gctcgcgcag cagcgcctcg
      361 cgctccgccc gtgccagcgc gttgagcgag tccgcggcgt cggccccagc ctgggccgcg
      421 cctgcgtgcg cgccaaggct gtagtccgac ggcaggtctg cgaggtccag ccggtcgctg
      481 gagcggcgcg cgatcatcgc gcgcagcgcg ttgcgcagct gccgcaggtt gcccggccag
      541 cggtgcgcgc agagcgcgtc caccagccgc tcgcttagcg agacgcagtg ggcgtcggcc
      601 gcctcctgcg cgaacaggtg gcggatgagc gcgcgcttgt cggcgcgctc atgcaagcgc
      661 ggcagcgtta ccaccagccc ctgcagccgg tagaagaggt cttcgcgaaa ctggccgcgt
      721 cggatcttgt cttccaggct gcacgcactc gccgcgacca cccgcacgtc gatggaagtc
      781 ggcgcctcgc ctgcgcgcag tactgcctcg ccgcgctcga gcaggtggag cacgcgcgcc
      841 tgcaaggcgc cgggcagctc gccgatctcg tcgagaaaca gcgtcccgcc ccgggcctgc
      901 gcaagcgcgc tttgcaggct cgcttcgggc aaggcggcgc aattcagcgc gacgaaaatc
      961 ctggtggcgc gctcgctcgc cgcatgcagc gcgcgcgcga agacttcctt gcccgtgccg
     1021 gtctcgccga cgatcaaggt cggcacggcc cgtgaggcga ctcgcttggc cgcgcggatg
     1081 ttgcgcgcca tgaccggatc gcccagaccg agctcgtcga gcgcgctcga ggacgcggcc
     1141 tcgggcatcg ggctcgcgcg ctgtcccgca gagctcgagc gctgtgccgg gtccggctcc
     1201 tgggccagcg cgaaaaagcg gccgccgtgg cgcatttcgt agatcggcag cgggtggagg
     1261 agcttcctgc ggctgcgtcc gagcagcgcg ggcagcgaga tgttgaacac gcgctcgagc
     1321 ggtgcaccgc tcatcaggtg cgcggcctgc ttgcagccga gctggaacag cgcgttgcga
     1381 tcgagcgcgg caatcgtgcc ggcggggctg aatgcgatca tgccttcgct cgacgtgccg
     1441 agcaggtccg gccggctgtg gaagcgcacg acgaaggcgt cgcggaagcg gtggaggaac
     1501 aggcgcttct cgatcgcctg cgccgacagg ttcaccagca ccagcgtgtg ctcttgcgcg
     1561 cggtccgatt cccccgaagc gtcgagcacg gcatagagct cgccgccggg gtcgaatatc
     1621 ggagccgcgc agcacgtgag cccggtgttg cgcgcgagga aatgctgctc gcgatgcacg
     1681 atcagcgcgg cgcgctcggc gaggcaggtt cccatcccgt tggtgccgcc gtggcgctcg
     1741 ctccacacgc cgcccggcac cagcccggcg cgcgacgccg catgcttgaa gctcgggtcg
     1801 ccgtagtagg aaagcactac cccgtcgcga tcggtgagga tgatcgagtg gcccgagccc
     1861 gcgagctggt gctgcaggtg cgccatctcg atctcggcga acgcaacgag ctcgaggctc
     1921 tgctccttgc gcaccatgag ctcctggtgg gagacgagcg ccggctgtcc gctcgactcc
     1981 ggatcgagtc cgtattcatc caggcaacgc tgccacgagc gcctcacgtg ctcgggtgcg
     2041 gtcgcatcaa gcgaggtacc gcgaatcacg gagtgcacga ggcgtgcgtg actaccgctg
     2101 ggcagcatga tgctctcgat aaattatatg cccgcggcgg tacggtcacg gcggcgacgc
     2161 gcggctcgcg ggaagttcat tttgcagctc gcgtcgctcg agcgcgacgt aggcatggtg
     2221 cacgttgcgc ggatgaatcg tctcgtagag cgcccagtgg cggtactcat gcactgcgac
     2281 gtttcgcggt gcctccagca ggctcgcgcc tgccgcgtaa gcctcttgcg tgtgtcgttc
     2341 cagcccgccc agataagcgg ccacggcttt gagatcggcc ggggatcctg cccacccgtg
     2401 cgccggaatc acacggcgcg ctggaatgcg ggataagcgc tgcagcgccc ggatccattc
     2461 ggcgaggttg ccgtcccggg tttcgggaac ccggccgaag ctcgccaagc cgcctgcaaa
     2521 gagcacgccc gattcgcggt cccagaccgc gatgcttccg ggcgcggcgg cgccgctctc
     2581 atcgacgagc tcgagccgcc ggccagcgac cgagagcagc tgcgagccgt tgaagagccg
     2641 ctccggacgc ggcacgcggg tcccggccat gatgtctgcg ccaagcgcgg cggtgcggct
     2701 ctcgaggcag cgctggcagc gctcggcgat gagccgtgac gcggcctcgt gaacgtacac
     2761 cgggacaccc tggtcctgaa aaacggcagc gccgaaaatc gcctcgtcca tcggccacgt
     2821 gagaatgagg atggcgagcg gctgcgcggt gcgtgcgcga attgcgcgca gcatgtgctc
     2881 ggcgaaagcg gcggacaggc cgctgtcgat gacgatcacg ccgcgcgggg cgatgatgaa
     2941 cccctggttg gcgatgtggc cccaattcgc gcggcttgcc tcctcttgcg cgccgaaaaa
     3001 tacatgcacg ccgtttccga cggcgaccgg ctcgggaaag ggcgctgcgg cgccgagcga
     3061 tgcggcgagg caagcagcgg cgagcgcggc gagagcgcgt gctagcatga tccttcgcgc
     3121 ccatgtggag gaaacggatc atgccgccgg tccatcgggt cgtctttgcg
//
LOCUS       DASUNT010000383         3712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1105958, whole genome shotgun sequence.
ACCESSION   DASUNT010000383 DASUNT010000000
VERSION     DASUNT010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105958"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1035)
                     /gene="hemC"
                     /locus_tag="VFX94_07765"
     CDS             complement(124..1035)
                     /gene="hemC"
                     /locus_tag="VFX94_07765"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX5768114.1"
                     /translation="MRKLVIATRRSRLALWQAEHVRKRLEALHRGLAVELLALSTRGD
                     ELIDRRLDQAGGKGLFVKELEQAMAQGRAELAVHSIKDVPAELPPGFALAAITAREDP
                     RDAFLSQKFKSLGEMPAGASVGTSSLRRAAQITERHPRLEIRLLRGNVDTRLARLDRG
                     DYDAIVLAAAGLERLGLAARATAHLQVEEMLPAPGQGALGIECLAARAEVAELLARLA
                     DRDTTACVRAERAVSRALGGSCSLPLAAYAELRSEKLRLRALVASSDGRRVIRCDVED
                     DAGAPEALGERAAGELRRQGAEEILGL"
     gene            complement(1032..1787)
                     /locus_tag="VFX94_07770"
     CDS             complement(1032..1787)
                     /locus_tag="VFX94_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX5768115.1"
                     /translation="MSPPATPLKVFIVDDEAPARERLKELLGDITAEVPTLLVGEARH
                     GVEAVELIPGSDAQVVLLDIKMPAMGGLEVARHLGRLEQPPRIVFVTAHDRHAVEAFE
                     LNALDYLLKPVRAERLAAALRKASVPEGEKLAKAAEEPREYLSVPERHRIALVPVRDI
                     LFLRAEQKYVTVRTRSREYLIEEPLVALEREFATRLVRIHRNCLVARAAIRGFERAPG
                     EEDEAHWLVQLDGVEEKLPVSRRQWPLVRELVT"
     gene            complement(1784..2833)
                     /locus_tag="VFX94_07775"
     CDS             complement(1784..2833)
                     /locus_tag="VFX94_07775"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5768116.1"
                     /translation="MHKNTVEEPYVTMVSIRPNVHPDVLPDFRNVGVIARILLAVNTA
                     VLAGALFASADVAQALARFVEAAAFVEPLLLVIIVALFALCGTLRRLPYRAGCAAVIA
                     LVATLLAAYHAAIGMGAAPGRLLLLSMLVAAALLAYFRLLWKAHSPALAEARLQALQA
                     RIRPHFLFNSLNAVLALIRRDPQRAERALEDLADLFRTLMSDARQFVRLSDEIALLER
                     YADLEQLRLGERLRITWDLDGAPVDALLPPMVLQPLLENAVYHGVEPGTGPADVLVRI
                     ERRGNRVLARIENPYVVNAQGQPEVLRAGNRMALENIRERLQLFFDAEARIATQAAGG
                     RYRVEIEIPYRAKAV"
     gene            2828..>3712
                     /gene="argH"
                     /locus_tag="VFX94_07780"
     CDS             2828..>3712
                     /gene="argH"
                     /locus_tag="VFX94_07780"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX5768117.1"
                     /translation="MQRRRTRSPWSARFAEPVDERVKRFTASVGFDRRLAKYDIQASL
                     AHAEMLAAQGLLARRDLAAIRRGLARIEREIDAGRFRWSLDAEDVHLNIERRLTALVG
                     DAGKRLHTARSRNDQVATDVRLWLRAEIDALAGLIAGLQAALVDQADRHAALIMPGFT
                     HLQVAQPVTFGHHLLAYVEMLERDRQRLAQARSRVNRLPLGAAALAGTSFRIDRDRVA
                     RALGFEGLCENSLDAVSDRDFAIEFCACAALAMVHLSRLAEELVLWANPRFGFVRLPE
                     AYCTGSSIMPQKKNPDVPE"
BASE COUNT          563 a         1328 c         1275 g          546 t
ORIGIN      
        1 aggtcctcga tctcgatcgc cgggaagaga tgcgcgcggc cgcccgctcc ttcgatcagc
       61 cgggccagat tcgccgcacg ctcgcccggg cgtgtcacca ggatgccacg accctccagt
      121 gcttcagaga ccaagaattt cttccgcacc ctgacggcgc agctcgcctg cagcccgctc
      181 gccgagcgcc tctggtgcgc cggcatcgtc ctcgacgtca caacggatca cgcgccggcc
      241 atcgctcgag gcgaccagcg cccgcaaccg gagcttttcg gatctcaatt ccgcataagc
      301 cgcgagcggt agcgagcagc ttccgccgag ggcgcggctc accgcgcgct cggcgcgcac
      361 gcaagccgtg gtgtcgcgat ctgccagccg cgcgaggagc tcggcgactt ccgcgcgcgc
      421 cgcgagacac tcgatgccga gcgccccctg ccccggcgcc ggcagcatct cttcgacctg
      481 gagatgcgcg gttgcccgcg cagcgaggcc aaggcgctcg agtcccgcgg cggcgaggac
      541 gatggcgtcg tagtcgccgc ggtcgagcct ggcgagccgc gtgtccacgt tgccgcgcag
      601 caggcgaatc tccaaccggg gatggcgctc ggtgatctgc gccgcgcgcc gcaggctcga
      661 agtgcccacg ctcgccccgg ccggcatttc gccaagagac ttgaattttt gcgatagaaa
      721 agcgtcgcgc ggatcctcgc gcgcggtgat cgccgccaat gcaaaccccg gcggcagctc
      781 ggccgggacg tccttgattg aatgcaccgc gagctcggcg cggccctgcg ccatcgcctg
      841 ctcgagctcc ttgacgaaga gccccttgcc gcccgcctgg tcgagccgcc ggtcgatcag
      901 ctcgtcgccg cgcgtggaga gcgcgaggag ctccacggcg aggccgcgat gcagcgcttc
      961 gagccgcttt ctcacgtgtt ccgcctgcca cagcgcgagc cggctgcggc gtgtcgcgat
     1021 cacgagcttc ctcatgtgac gagctctcgc acgagcggcc actgccgccg gctcaccggc
     1081 agcttctcct ccacgccgtc gagctgcacc agccagtgcg cctcgtcctc ctcgccgggc
     1141 gcgcgctcga agccgcggat ggcggcgcgc gcgaccaggc agttgcgatg gatgcgcacc
     1201 aggcgcgtcg cgaactcgcg ctcgagggcg acgagcggct cctcgatcag gtactcccgg
     1261 ctgcgcgtgc gaaccgtgac gtatttctgc tcggcgcgca ggaacaggat gtcgcgcacc
     1321 ggcacgagcg cgatgcggtg gcgctcgggc accgagaggt actcgcgcgg ctcctcggcc
     1381 gctttggcga gcttttcgcc ctcggggacc gacgccttgc gcagcgccgc ggcgaggcgc
     1441 tcggcgcgca caggcttcag caggtaatcg agcgcgttca gctcgaacgc ctcgacggcg
     1501 tggcggtcgt gcgcggtgac gaacacgatg cgcggcggct gctcgagtcg gcccagatgc
     1561 cgcgcgacct cgagcccgcc catcgccggc atcttgatgt cgagcagcac gacctgcgcg
     1621 tcgctcccgg gaatcagctc gaccgcctcg acgccgtggc gcgcctcgcc caccaggagg
     1681 gtcggcacct cggcggtaat gtcgccgagc agctctttca ggcgctcgcg cgccggcgcc
     1741 tcgtcgtcga cgatgaagac cttgagcggg gtcgcgggcg gactcatacg gctttcgccc
     1801 ggtagggtat ctcgatctcg acgcggtagc ggccgccggc ggcctgcgtg gcgatccgcg
     1861 cctcggcgtc gaagaaaagc tgcagccgct cgcggatgtt ctcgagcgcc atgcggttcc
     1921 cggcgcgcag cacttcgggc tgcccctggg cgttcaccac gtaaggattc tcgatgcgcg
     1981 cgagcacgcg gttgccgcgg cgctcgatgc gcaccagcac gtcggccggg ccggtgcccg
     2041 gctccacgcc gtggtacacc gcgttctcga gcagcggctg cagcaccatg ggcggcagga
     2101 gcgcgtccac cggcgcgcca tcgagatccc acgtgatgcg cagccgctcg cccaggcgca
     2161 gctgctcgag atcggcgtaa cgctcgagca gcgcgatctc gtcggaaagc cgaacgaact
     2221 gccgcgcgtc cgacatcagg gtgcggaaga gatctgccaa gtcttcgagc gcgcgctcgg
     2281 cgcgctgcgg atcgcggcgg atgagcgcga gcaccgcgtt caggctgttg aaaaggaaat
     2341 gcgggcgaat gcgcgcctgc agcgcctgaa ggcgcgcctc ggcaagcgcg ggcgagtgcg
     2401 ccttccagag caggcgaaag taggcgagga gcgccgccgc cacgagcatc gatagaagga
     2461 gcaggcgccc cggcgccgcg cccatgccga tcgccgcgtg gtaggcggcg agaagcgttg
     2521 cgacgagcgc aatgacggcc gcgcatcccg cccggtaggg cagccggcgc agcgttccgc
     2581 aaagcgcgaa gagcgccacg atgatgacga gcagcagcgg ctcgacgaac gcggccgcct
     2641 ccacgaagcg cgcgagcgct tgcgcgacat cggccgaagc gaacagcgcg ccggcgagca
     2701 ccgccgtgtt caccgcgagc agaatgcgcg cgatgacgcc gacgttgcgg aagtccggca
     2761 acacgtccgg gtgaacgttt ggtcttatac tgaccatggt tacgtagggc tcttcaaccg
     2821 tattcttatg caaagaagaa gaactcgctc gccctggtcc gcccgcttcg ccgagcccgt
     2881 cgacgagcgc gtgaagcgtt tcacagcttc ggtcggcttc gaccggcgtc tcgccaaata
     2941 tgacatccag gcatcgcttg cccacgccga aatgctcgcg gcccagggcc tgctcgcgcg
     3001 ccgcgacctg gccgcgatcc ggcgcgggct cgcgcgcatc gagcgggaaa tcgacgccgg
     3061 ccgcttccgc tggtcgctcg acgcggaaga tgtgcacctc aatatcgagc ggcggctgac
     3121 cgcgctcgtg ggcgatgccg ggaagcgttt gcacaccgcg cgctcgcgca acgaccaggt
     3181 ggccaccgac gtgcgcctgt ggctgcgcgc cgagatcgac gcgctcgccg gcctgatcgc
     3241 cggtctgcag gccgcgctcg tggaccaggc ggaccggcat gccgctctca tcatgccggg
     3301 cttcacccac ctgcaggtgg cgcagccggt gacttttggc caccacctgc tcgcctacgt
     3361 cgagatgctg gaacgcgacc ggcaacgctt ggcccaggcg cgctcgaggg tgaaccggct
     3421 gccgctcggc gccgcggcgc ttgccggcac gagctttcgc atcgaccgcg accgcgtcgc
     3481 acgcgcgctc gggttcgaag ggctgtgcga gaactcgctc gatgcggtgt cggaccgcga
     3541 tttcgcgatc gagttctgcg cgtgcgccgc gctcgccatg gtgcacctct cgcgcctggc
     3601 ggaagagctc gtgctttggg caaacccgcg cttcggcttc gtgcggctgc ccgaggcgta
     3661 ttgcaccggc tcgtcgatca tgccgcaaaa gaagaacccg gacgtgcccg ag
//
LOCUS       DASUNT010000384         2917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1282762, whole genome shotgun sequence.
ACCESSION   DASUNT010000384 DASUNT010000000
VERSION     DASUNT010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282762"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VFX94_07785"
     CDS             complement(<1..343)
                     /locus_tag="VFX94_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5768118.1"
                     /translation="MLRALAPDVRVREVWAWSMYDFATSAYTTVVITAVFSAYFVAGV
                     AGNAAWATFAWTAALSVSYAAILVTGPLIGAWADAHAAKKRLLFATTLGCVLFTAALC
                     FAQPGAVALSLV"
     gene            complement(368..691)
                     /locus_tag="VFX94_07790"
     CDS             complement(368..691)
                     /locus_tag="VFX94_07790"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX5768119.1"
                     /translation="MAHYMFQASFTSEAWAKMVQEPQNREQSIRPMIEKLGGKLIGYW
                     FAFGESDAVVIVQMPDNVSAAAASLAAAAGGAVKSLKTTPLMSVEEGMQAMRRAGEAG
                     YRAPR"
     gene            complement(802..1059)
                     /locus_tag="VFX94_07795"
     CDS             complement(802..1059)
                     /locus_tag="VFX94_07795"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEX5768120.1"
                     /translation="MSRVASMPSFFGMGEDPMAGLARFAAEHKLSASSFTAIGAFSEA
                     LLGHFDWEKKDYERIPRADRGPRSRRRCHARRRWRLRDHLP"
     gene            1148..>2917
                     /locus_tag="VFX94_07800"
     CDS             1148..>2917
                     /locus_tag="VFX94_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX5768121.1"
                     /translation="MQQAVKALEGGQKHDGRLALAQVLDWLVADAMVAAEAAEQLKKE
                     RRYYRGTTHPLVIVAQQKWKKGATALTLGVLSEWLAKRVGMEYLHIDPLKIDFAGVTE
                     IMSSAYATRFRILPVALTAREVVIATAEPGVRDWEPELSRIIKREIKRVIANPLDIER
                     YQVEFYNLAKSIKGAGLKHAGTSGLSNFEQLVELGKTAGDKGQPLDANDAHVVRVVDW
                     LWQYAFEQRASDIHIEPRREQGIVRFRIDGVLHQVYQIPAPVLAAMTSRIKILARMDV
                     VEKRRPQDGRIKTRSGEQDVELRISTLPTAFGEKLVMRIFDPEVLVRDFSELGFSQED
                     QRRWKEMTDRPNGIVLVTGPTGSGKTTTLYSTLKSLATSEVNVCTIEDPIEMIEPAFN
                     QMQVVSAIELGFAEGVRALMRQDPDIIMVGEIRDLETAEMAVQAALTGHLVLSTLHTN
                     DAPSAIARLLELGVPAYLLNATLNGVMAQRLVRTLCPHCKQAIEMNRAEDHDAWNALV
                     SPWKSKQPARLFKPVGCLECRMTGYIGRVGIYEVLVFSQAIKGLVNQNADLVALREQA
                     FKEGMKPLRISGAMKVGAGLTTIE"
BASE COUNT          533 a          954 c          984 g          446 t
ORIGIN      
        1 aaaccagaga aagggcgacc gcgccgggtt gcgcgaagca gagagccgcg gtgaagagca
       61 cgcagccgag cgtggtggcg aagagcagcc gcttcttggc tgcgtgcgcg tccgcccacg
      121 ccccgatcag cggtccggta acaaggattg ccgcgtagga cacggagagc gccgcggtcc
      181 acgcgaacgt cgcccatgct gcgttaccgg cgacgccggc aacgaaatag gcgctgaaaa
      241 ccgccgtgat gacaacggtg gtgtaggccg aggtcgcgaa atcgtacatg ctccacgccc
      301 agacctcgcg cacgcgcaca tcgggggcca gggcgcggag cataacgaac ggggtggccg
      361 ccgcttttca gcggggcgcg cggtagccgg cctcgcccgc cctgcgcatc gcctgcatgc
      421 cttcttcgac cgacatgagc ggcgtcgtct tcagcgattt gaccgcgccg ccggccgctg
      481 cagcgagcga cgcggccgcc gccgacacgt tgtcgggcat ctgtacgatg acgactgcgt
      541 ccgattcgcc gaacgcgaac cagtagccga tgagcttgcc gccgagcttc tcgatcatcg
      601 gccggatcga ctgctcgcga ttctgtggct cctgcaccat cttcgcccac gcttcggacg
      661 taaaggacgc ctggaacatg tagtgcgcca tgactgcctc ctctgtcccc gggatgggga
      721 ttggcagtct atttcaaagc agcgaggtag gccgcgatgt cgcgtgcgtc gcgctcgctt
      781 acccgaggcc aggcattgcg actacggaag atgatctctt agtcgccagc gtcgtcgagc
      841 gtgacatcgc cggcgagagc gaggacctcg gtctgcccgc gggatgcgct cgtagtcctt
      901 cttctcccag tcgaagtggc cgagcagcgc ctcgctgaac gcgccgatcg ccgtaaagct
      961 cgatgcgctc agcttgtgct ccgcggcgaa gcgcgcgagc ccggccatcg ggtcctcgcc
     1021 cataccgaaa aacgagggca tagacgcgac cctgctcatt gatcaacttc acctgcatgc
     1081 cgcacagccc cccgcaacga acgtgccgcc ggagcggctt tgggctttgc ggtatagtct
     1141 ttcggctatg caacaagcag ttaaggcgct ggagggaggt caaaaacacg acgggcgcct
     1201 cgcgctcgcg caggtgctcg actggctggt cgcggatgcg atggtggccg ccgaggcggc
     1261 ggagcagctc aagaaggagc ggcgctacta ccgtggcacg acgcatccgc tggtgatcgt
     1321 cgcgcagcag aaatggaaga aaggcgccac agcgctaacg ctcggcgtgc tgagcgagtg
     1381 gctggcgaag cgcgtgggca tggaatacct gcacatcgat ccgctgaaga tcgacttcgc
     1441 cggcgtcacc gagatcatgt cctcggcgta cgcgacccgc ttccgcatcc tgccggtcgc
     1501 gctcacggcg agggaagtgg tgattgccac cgccgagccg ggcgtgcgcg actgggagcc
     1561 tgagctctcg cgcatcatca agcgcgagat caagcgtgtg atcgcgaacc cgctcgacat
     1621 cgagcgctac caggtcgagt tctacaacct cgccaagtcg atcaagggcg cgggcctgaa
     1681 gcacgccggt acctccgggc tgtccaactt cgagcagctg gtcgagctcg gcaagaccgc
     1741 gggcgacaaa gggcaaccgc tcgacgccaa cgacgcgcac gtggtgcgcg tggtcgactg
     1801 gctgtggcag tacgccttcg agcaacgcgc gagcgacatc cacatcgagc cgcggcgcga
     1861 gcagggcatc gtacgcttcc ggatcgacgg cgtgctgcac caggtgtacc agatcccggc
     1921 cccggtgctg gcggcgatga cgagccgcat caagatcctc gcgcgcatgg acgtggtcga
     1981 gaagcgccgt ccgcaggacg ggcgcatcaa gacgcgctcg ggcgagcagg acgtcgagct
     2041 gcgcatctcg acgctgccga ccgcgttcgg agagaagctc gtgatgcgca tcttcgaccc
     2101 cgaggtgctg gtgcgcgact tctccgagct cggcttctcg caggaggacc agcgccgctg
     2161 gaaggagatg accgaccggc cgaacggcat cgtgctcgtc accgggccga ccggctcggg
     2221 caagaccacg acgctctact cgacgctcaa gagcctcgcc acgagcgagg tcaacgtctg
     2281 cacgatcgag gacccgatcg agatgatcga gccggcgttc aaccagatgc aggtcgtgag
     2341 cgcgatcgag ctcggcttcg ccgagggcgt gcgcgcgctc atgcgccagg acccggacat
     2401 catcatggtg ggggagatcc gcgacctgga aaccgcggag atggcggtgc aggcggcgct
     2461 caccggccac ctcgtgctct ccacgctgca taccaacgac gcgccgagcg cgatcgcgcg
     2521 gttgctggag ctcggcgtgc ccgcctatct cctcaacgcg acgctcaacg gcgtgatggc
     2581 gcagcgcctg gtgcgcacgc tgtgcccgca ctgcaagcag gcgatcgaga tgaaccgcgc
     2641 cgaggaccac gacgcctgga acgcgctcgt gtcgccgtgg aagtccaagc agccggcgcg
     2701 gctcttcaag ccggtcggct gcctcgagtg ccgcatgacc ggctacatcg gccgggtcgg
     2761 catctacgag gtgctggtgt tctcgcaggc catcaaaggc ctcgtgaatc agaacgcgga
     2821 cctcgtggcg ctgcgcgagc aggcgttcaa ggaaggcatg aagccgctgc gcatctccgg
     2881 cgcgatgaag gtgggcgctg gattgacgac gatcgag
//
LOCUS       DASUNT010000385         2205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_54217, whole genome shotgun sequence.
ACCESSION   DASUNT010000385 DASUNT010000000
VERSION     DASUNT010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54217"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..810)
                     /locus_tag="VFX94_07805"
     CDS             complement(127..810)
                     /locus_tag="VFX94_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5768122.1"
                     /translation="MISLYELKGKGERRYSLFSWRTRMALKHKGLEFESCPVRMSDKA
                     AIAFSGGKTVPVIRDEDTVVRDSWKIAEYLESRYADAPPLFGGEIGRGVSAAFNTWVD
                     RAVVPAMLPVIAADIHERVDPDDESYFRQTMEKALKTTLEESRARRDAAVLNLGRVLS
                     PMQEACKRQPFISGASAAYADYILFSVFQWARVMSPQELLGPEDPLCRWRERMLELFG
                     GFARNVPTA"
     gene            complement(807..>2205)
                     /locus_tag="VFX94_07810"
     CDS             complement(807..>2205)
                     /locus_tag="VFX94_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX5768123.1"
                     /translation="RDFHQRALSLARGLAGLGVGKGDVVAVQLPNIPEYLLSYAAICA
                     LGATLQPVHMPYRRAELATLLKHSGTRVFLCLSRFKEESPAKEAASVVDQVVALGEPV
                     DGAKAFGSLTAGARLERVSEGSPEDRFLLLYTSGTTDEPKGVPHAQRGFLANAMASVP
                     EMEIAKDEVILSAAPLTHLYGLYAYHLCLCSGAAMSLLPAFTPPALANIIEKHRVDCV
                     FAGPAHFKPLLDGGLLERHDFSSLRFACLSGSPVPPELAAAVERKLPKGKTIQLWGMT
                     ELQAGSFSRPRDPADVRHGTAGAATPGTELRVVGERLQVQGISLFSGYLKNPKATREA
                     FSADGWFETGDTAELSAAGHLRFTGRVKEIINRGGVKYSPLDVETIIDRMPGIARSAI
                     VPFPDAVLGERACVFVQPALGAARPTLEAIIRALDSAGVAKFKWPERLELVEAMPITP
                     TQKVMRGRLRELLKR"
BASE COUNT          392 a          720 c          726 g          367 t
ORIGIN      
        1 ggcaatgcgc gctggggaag gcgcgcttga cgtcctgctc cttggcgccg agcgcgatgt
       61 ccttgatgtc gagcgctgcc agcgccggcg gtgtgaacac gagcagtgtg acaagaaaga
      121 gatttctcat gcagtcggta cgttgcgggc gaagccgccg aagagctcga gcatgcgctc
      181 gcgccagcgg cagagcgggt cctcgggccc cagcagctcc tgtgggctca tgacgcgcgc
      241 ccactggaac accgagaaga gaatgtagtc ggcgtacgcg gcgctcgcgc cggagatgaa
      301 aggctggcgc ttgcacgcct cctgcatagg cgagagcacc ctgccgaggt tgagcacggc
      361 cgcgtcgcgc cgcgcgcgcg actcttccag cgtggtcttc agcgccttct ccatcgtctg
      421 gcgaaaatag gactcgtcat cggggtcgac gcgctcatgg atgtcggccg cgatcaccgg
      481 caacatcgct ggcacgacgg cgcggtcgac ccaagtgttg aaggcggccg acacgccgcg
      541 gccgatttcg ccgccgaaaa gcggcggggc atcggcgtag cggctctcca ggtattccgc
      601 gatcttccag gagtcgcgca ctacggtgtc ctcatccctt atcaccggaa cggtctttcc
      661 gcccgagaaa gcgatcgccg ccttgtctga catgcgcacc gggcacgact cgaactccag
      721 gcccttgtgc ttgagcgcca tgcgtgtgcg ccaggagaag agactgtagc gtcgttcccc
      781 cttgcccttc agctcgtaca aggaaatcat cgcttcagca gctctcgcag ccggccccgc
      841 atgaccttct gcgtcggcgt gatgggcatc gcctcgacga gctccaggcg ctcgggccac
      901 ttgaacttcg ccacgccggc gctatcgagc gcgcggatta tcgcttccag cgtcggccgc
      961 gcggcgccaa gagccggctg cacgaacacg caggcgcgct cgccgagcac cgcgtccgga
     1021 aagggcacga tggctgagcg cgcaatgccc ggcatacggt cgatgatcgt ctccacatcg
     1081 agcggcgagt atttcacgcc gccgcggtta ataatctcct taacgcgccc ggtgaagcgc
     1141 agatgaccgg ccgcggacag ctccgcggtg tcgcccgtct cgaaccagcc gtcagcgcta
     1201 aaggcttcgc gcgtggcctt cgggtttttc agatagccgg aaaaaagaga aatcccctgc
     1261 acctgcagcc gttcgcccac gacgcgaagc tcggttccgg gggtggcggc gcccgccgtg
     1321 ccgtggcgca catcggcggg gtcgcgcggc cgcgagaagg agcccgcctg cagctctgtc
     1381 atgccccaga gctggatcgt cttgcctttg ggcagcttcc tctccaccgc cgcggcaagc
     1441 tcgggcggca ccggacttcc ggagaggcag gcgaagcgca gcgacgagaa gtcgtgccgt
     1501 tcgagcagcc cgccgtccag cagcggcttg aaatgcgccg gcccggcgaa cacgcagtcc
     1561 acccgatgct tttcaataat gttcgcaaga gcgggcggtg tgaatgcggg caacaacgac
     1621 atcgcggcac cggagcagag gcaaagatga tacgcgtaca gaccgtagag gtgagtgagg
     1681 ggcgccgcgg acagaatcac ctcgtctttc gctatttcca tctccggtac cgaggccatc
     1741 gcgttggcaa gaaagccgcg ctgcgcgtgc ggcacgcctt tcggttcatc cgtggtgccc
     1801 gacgtataga gaagtaggaa gcgatcttcg ggcgagcctt cgctcactcg ctcaagccgc
     1861 gcgcctgccg tgagcgatcc gaaggctttt gctccgtcga ccggctcgcc gagcgcgacg
     1921 acctggtcta ccaccgaagc ggcttccttg gctggagatt cttccttgaa gcgcgaaagg
     1981 cacaggaaga cgcgggttcc ggagtgcttg agcagggttg caagctcggc gcgccggtac
     2041 ggcatatgaa ccggttgcag cgtcgcgccg agcgcgcaga tcgcggcgta cgagagcagg
     2101 tattccggga tgttgggcag ctggaccgct acgacatcgc ccttgccaac gcccagcccg
     2161 gcgagccctc gggcaagact tagggcccgc tggtgaaagt ctcgg
//
LOCUS       DASUNT010000386         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_755819, whole genome shotgun sequence.
ACCESSION   DASUNT010000386 DASUNT010000000
VERSION     DASUNT010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755819"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /gene="iolD"
                     /locus_tag="VFX94_07815"
     CDS             complement(<1..1356)
                     /gene="iolD"
                     /locus_tag="VFX94_07815"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004531192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HEX5768124.1"
                     /translation="MAEALVRFLMNQAVEIPGDDPAPLFGGVWAIFGHGNVAGLGPAL
                     HAVRRKLPTLRAHNEQAMAHAAIAFAKAHARRRMMACTSSIGPGASNMVTAAAVAHVN
                     RLPVLFLPGDVFASRRPDPVLQQLEDFRDPGASVNDCFRPVSRYFDRIMRPEQLLVSL
                     PAALRVLLDPADCGPVTLALPQDVQTEAYDYPAEFFRRRVNRIRKPLPDASEAEAVSK
                     ALRAAKRPLVIAGGGVKYALAEKRLAAFAQAHGLPVAETQAGKGSLAWDHACNVGAIG
                     VTGSQAANALAARADLVLAIGTRLGDFATGSRTLFPAAKLVQLNVEAFDAAKHGALPL
                     VADAEAGLEALGRALEGWRAAAAWTAEAGLLSRRWNGEVERIIAPEGNRPKAGNPSDA
                     QVLGAVNSLLPKNSAVVCAAGGLPGELHKLWRATDSTSYHLEYGYSCMGYEIAGGLGV
                     KL"
     gene            1416..>2249
                     /gene="iolG"
                     /locus_tag="VFX94_07820"
     CDS             1416..>2249
                     /gene="iolG"
                     /locus_tag="VFX94_07820"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEX5768125.1"
                     /translation="MLEIAQFGAGRIGQIHAANIAAMKDVRLRYIVDVNPDAAQKLAA
                     LHAAKVASERDALADPGVSAVLIASSTDTHARLAIAAARAGKAIFCEKPIDLSLKRVD
                     GCLAEVEKAGVPMFVGFNRRFDPSFSALKARLDAGEIGALEQVVISSRDPGAPPLSYI
                     RVSGGMFRDMTIHDFDMARWLLGEEPVEVFAMGECLVDRKIGAAGDIDSAMVLLRTAS
                     DRMCHINNSRRAAYGYDQRIEVFGAKGMLRAENIGETTVEHFGPRGTVADRPLDFFLE
                     RY"
BASE COUNT          376 a          771 c          746 g          356 t
ORIGIN      
        1 gagcttgacg ccgagcccgc ccgcgatctc gtagcccatg caagagtagc cgtactccag
       61 gtggtagctc gtcgagtcgg ttgcgcgcca gagcttgtgc agctctcccg gcagtccgcc
      121 ggcggcacac accacggccg agttcttcgg aagaagcgag tttacggcgc ccaggacttg
      181 ggcatcggac ggatttcccg ccttcgggcg atttccttcc ggcgcgatga tgcgctccac
      241 ttcgccattc catctgcgcg acagaagccc ggcttccgcg gtccaggccg ccgcagcgcg
      301 ccagccctcg agcgcgcgcc caagcgcctc gagacccgct tccgcatccg cgacgagggg
      361 gagcgcgccg tgcttggccg catcgaaagc ttcgacattg agctgcacga gctttgccgc
      421 cgggaaaagc gtgcgcgagc cggtggcgaa atcgccgagg cgcgtgccga ttgcaagcac
      481 gagatccgcc cgcgcggcaa gcgcgttcgc cgcctgcgag ccggtgacgc cgatcgcgcc
      541 gacgttgcac gcgtgatccc acgcgaggct tccctttccc gcctgcgtct cggccaccgg
      601 cagcccgtgc gcttgcgcaa aagcggcaag ccgcttttct gcgagcgcgt acttgacccc
      661 gccgcccgcg atgacgagag gcctcttggc agcgcgaagc gctttggaga ccgcttcggc
      721 ttcgctcgcg tccggcaatg gcttcctgat tcgattcaca cggcggcgga agaattcggc
      781 cggatagtcg tacgcctcgg tctgcacgtc ctgcggcagc gcaagcgtga ccgggccgca
      841 gtcggccgga tcgagcagca cgcgaagcgc agccggcagg ctgacgagga gctgctccgg
      901 gcgcatgatg cggtcgaagt aacgcgagac cggccggaag cagtcgttca ccgaggcgcc
      961 cgggtcgcgg aagtcctcga gctgctggag caccgggtcc gggcggcggc tcgcaaagac
     1021 gtcgccggga agaaagagca cgggcaggcg gttcacatgc gccaccgccg ctgccgtcac
     1081 catgttgctc gcgcccgggc cgatcgagct cgtgcacgcc atcatgcgcc ggcgcgcatg
     1141 cgcctttgcg aacgcgatcg cggcatgcgc catcgcctgc tcgttatgtg cgcgcagcgt
     1201 cggcagcttt cgacgcacgg catgcagggc gggcccgagg ccggcgacgt tgccgtggcc
     1261 gaagatcgcc cagacgccgc cgaacaaggg cgcgggatcg tctccgggaa tttccaccgc
     1321 ctgattcatg agaaaccgaa ccagagcttc cgccatggtg atgcgaaccg ctgccacgtg
     1381 cctcccctct cccgatttat catacacaaa caagaatgct ggaaatcgcc cagttcgggg
     1441 ccggacgcat cggccagatc catgctgcca acatagccgc catgaaggac gtacggctgc
     1501 gatacatcgt ggacgtaaac cccgatgcag cgcaaaagct tgccgcgctt catgccgcta
     1561 aggtcgcgtc cgagcgcgat gcgcttgccg atcccggggt gagcgcggtg ctcatcgcct
     1621 cgtcgaccga cacgcatgcg cgcctcgcga tcgctgcggc gcgcgcaggc aaggcgatct
     1681 tctgcgaaaa gccgatcgat ctcagcctga aaagggtgga cgggtgcctg gctgaagtgg
     1741 aaaaggctgg cgtgccaatg ttcgtcggct tcaaccgccg cttcgacccg agcttcagcg
     1801 cgctcaaggc tcgcctcgac gccggcgaga tcggcgcgct cgagcaggtc gtgatctcga
     1861 gccgcgatcc cggggcgccg cccctttcct acatcagggt ctcgggcggc atgttccgcg
     1921 acatgacgat ccacgatttc gacatggcgc gctggctgct cggcgaggag ccggtcgagg
     1981 tgttcgcgat gggcgagtgc ctggtcgacc gcaagatcgg cgcggcgggc gacatcgact
     2041 cggcgatggt gctcttgcgc accgcctcgg accgcatgtg ccacatcaac aactcgcgcc
     2101 gcgccgccta tggctacgac cagcgcatcg aggtgttcgg ggcgaaaggc atgctgcgcg
     2161 ccgagaacat cggcgagacc acggtggagc acttcggccc gcgcggcacc gtggcggacc
     2221 ggccgctcga tttcttcctc gagcgctac
//
LOCUS       DASUNT010000387         2497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1018652, whole genome shotgun sequence.
ACCESSION   DASUNT010000387 DASUNT010000000
VERSION     DASUNT010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018652"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..286)
                     /locus_tag="VFX94_07825"
     CDS             complement(44..286)
                     /locus_tag="VFX94_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768126.1"
                     /translation="MSKADDMRQAYAALTRGDWQAAHRIVQSDEDSRLSCWAHGIVHV
                     MEGDLPNARYWYRKAGRAFTEDVAAEIAALGSELNA"
     gene            335..871
                     /locus_tag="VFX94_07830"
     CDS             335..871
                     /locus_tag="VFX94_07830"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5768127.1"
                     /translation="MARPRRKAPPLTVSRAALLARGSDAEFRRLIHDLIAYGHKLDAC
                     RDAFAAIAGISGAQYEILMLVSRADGLSIGEVAARMHRSGAFITIEVNKLVGAGILEK
                     CSDPNDGRRVLLRCSEKGVALLERMAPYQRRVNDVLFEPLDAKRFRALRALAAELVAC
                     GDRAVAMLELLLHEAEAA"
     gene            868..1236
                     /locus_tag="VFX94_07835"
     CDS             868..1236
                     /locus_tag="VFX94_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEX5768128.1"
                     /translation="MSISYFVRYDVAVSEVSAFLERYRSVHVPLVAAWPGLQRMLIHT
                     PVDWTDAFPVNRGRAVLMAQLEFESEEAMNRAFASRERAEAREDFKRFMTFEGTVIHQ
                     AMRTEEVWRSPWLDGNEEQA"
     gene            1233..1619
                     /locus_tag="VFX94_07840"
     CDS             1233..1619
                     /locus_tag="VFX94_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEX5768129.1"
                     /translation="MMKTKQHLEFVKVDLQSGWEVPPGYPSGFYQKVLASDLDEKNKR
                     GSRTRLLKIEPGAFSTEPFVHDHWEEVYVVQGDLVVGNDKQGKGGTQCFAPTYACRPP
                     GVHHGPFTSRGGCILHEIHYYAEDRK"
     gene            1600..>2497
                     /locus_tag="VFX94_07845"
     CDS             1600..>2497
                     /locus_tag="VFX94_07845"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5768130.1"
                     /translation="MLKTGNEHLESLRDGRVVYIGSERVADVTTHPAFRNAARTVASI
                     YDMKIAERDAMSFEEAGERFSIYFLQPKARDDLARRTRGHRMIADLTYGMFGRSPDHV
                     ASFVTGMAMNSAVFGTFEKNLLAYYEHLRREDAYAVYAVIPPQAARNPEFYVKQNIPV
                     PTLRVVKEDDDGVVISGMKMLATGAVFANEIWIGNLIPLAPNQLAESITCAIPVSAPG
                     VTLWARKPYERQAQSEFESPLAWRFDETDSMVMCDSVKVPWERVFVHMDAVLAREIYI
                     KTPGHCYGNHQSNVRFHAKMQLI"
BASE COUNT          441 a          819 c          836 g          401 t
ORIGIN      
        1 gagcctctgc cgcagcagga gcgctacgcc agcgagaggc tacctaggcg ttcagctccg
       61 acccgagggc cgcgatttcc gccgcgacat cctcggtaaa ggcgcggcct gccttgcggt
      121 accagtagcg cgcgttcggc aggtcgccct ccatcacatg cacgatgccg tgcgcccagc
      181 aggaaaggcg cgaatcttcg tcggactgca cgattctgtg tgccgcctgc cagtcacccc
      241 tggtgagcgc cgcgtacgcc tggcgcatgt cgtcagcttt ggacattagc taattgacaa
      301 aggtaattgc ccgggcggag aatcctcgcg ccccatggcg cgtccccgca ggaaggctcc
      361 accgctcacg gtatcacgcg cggcgctgct cgcgcgcggc tcggacgcgg agttccgccg
      421 cctgatccac gacctcatcg cctacggcca caagctcgac gcctgccgcg acgccttcgc
      481 cgcgatcgcc ggcatctcgg gggcgcagta cgagatcctc atgctggtaa gccgcgccga
      541 cggcctgtcg atcggggagg tggccgcacg catgcaccgc tccggcgcct tcatcaccat
      601 cgaggtgaac aagctggtcg gggcagggat cctcgaaaag tgctccgatc cgaacgatgg
      661 caggcgagtg ctcttgcgtt gcagcgaaaa gggcgttgca ttgctggaac gcatggcgcc
      721 ctatcagcgc cgcgtgaacg acgtgctgtt cgagccgctc gacgcgaagc gctttcgcgc
      781 gctgcgtgcg cttgccgccg agctcgtggc ctgcggcgac cgcgcggtgg cgatgctcga
      841 gttgctgctg cacgaggcag aggcggcgtg agtatttcgt acttcgtgcg ctatgacgtc
      901 gcggtttccg aggtgagcgc gttcctcgag cgctaccgca gcgtgcacgt tccgctggtc
      961 gcggcctggc ccggcctgca gcgcatgctg attcacacgc cggtggactg gacggacgcc
     1021 ttcccggtaa accgcggcag ggcggtgctc atggcgcagc tcgagttcga gagcgaggag
     1081 gcgatgaacc gggcgttcgc ttcgcgcgag cgcgccgagg cgcgcgagga cttcaagcgc
     1141 ttcatgacct tcgaaggaac ggtgatccac caggcgatga gaaccgagga agtctggcgc
     1201 tcgccctggc tggacgggaa cgaggagcaa gcgtgatgaa gacgaagcag catctcgagt
     1261 tcgtaaaggt cgacttgcag agcggctggg aagtgccgcc gggctatccg agcgggttct
     1321 accagaaggt cctcgcttcc gacctcgacg agaagaacaa gaggggaagc cgcacgcgcc
     1381 tgctcaagat cgagccgggc gcgttctcta ccgagccttt cgtccacgac cactgggaag
     1441 aggtgtacgt cgtgcaaggc gacctcgtgg tgggtaacga caagcaggga aagggcggca
     1501 cgcaatgctt cgccccgacc tacgcctgcc gcccgccggg cgtccaccac ggtccgttca
     1561 cttcccgcgg cggctgcatc ctccacgaga tccactacta tgctgaagac cggaaatgaa
     1621 catctagagt cgctgcgcga cggccgggtg gtctacatcg gctccgagcg cgtggccgac
     1681 gtcacgacgc acccggcgtt caggaacgct gcgcgcaccg tcgcctcgat ctacgacatg
     1741 aagatcgccg agcgcgacgc gatgagcttc gaggaggcgg gcgagcggtt ctcgatctac
     1801 ttcctgcagc cgaaagcgcg cgatgacctc gcgcggcgaa cgcgcggcca ccgcatgatc
     1861 gcggacctga cgtacggcat gttcggccgc tcgcccgacc acgtcgcgag cttcgtgacc
     1921 ggcatggcga tgaattccgc cgtgttcggc acgttcgaga agaacctgct ggcctactac
     1981 gagcacctgc gccgcgagga cgcctacgcg gtctacgccg tgatcccgcc gcaggcggcg
     2041 cgcaacccgg agttctacgt caagcagaac atcccggtgc cgacgctgcg cgtggtgaag
     2101 gaggacgacg acggcgtggt gatcagcggg atgaagatgc tcgccaccgg cgcggtgttc
     2161 gccaacgaga tctggatcgg caacctgatc ccgctcgcgc ccaaccagct cgccgagtcg
     2221 attacgtgcg cgatcccggt gagcgcgccg ggcgtgacgc tgtgggcgcg caagccctac
     2281 gagcgccagg cgcagagcga gttcgagagc ccgctcgcct ggcgcttcga cgagaccgac
     2341 tcgatggtga tgtgcgacag cgtcaaggtg ccgtgggagc gcgtgttcgt gcacatggac
     2401 gcggtgctgg cgcgcgagat ctatatcaag accccgggcc actgctacgg caaccaccag
     2461 tcgaacgtgc gcttccacgc caagatgcag ctcatcg
//
LOCUS       DASUNT010000388         2796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_756340, whole genome shotgun sequence.
ACCESSION   DASUNT010000388 DASUNT010000000
VERSION     DASUNT010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756340"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1773)
                     /locus_tag="VFX94_07850"
     CDS             complement(<1..1773)
                     /locus_tag="VFX94_07850"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEX5768131.1"
                     /translation="MKRTALVALGAVAVLVGLYAAAGYWLAPGYVRDALAALAAERAL
                     TLDVADVRARPFELRVELDDVALRGSQGRVFAQAQSASADLAWASLWRRGWIFEQVRV
                     IAPQVEVGMLPAFGKAREEAGAAAVTVRQLAVEGGVLRDSPRDVALEALTLRASDLST
                     LDAAAGNYEAAGRLAGGAGQFASRGTVALAPLAAEGTLTVAALGAGQLFAEAHGQLKG
                     SARYVYRSGELELRDVSITGNRLAYAGIELPEATVTAQSVSLPLQSPVQVTAQATVAP
                     QGKLSAQGSVAISPLRLDMKVEANALPLAQAQRWLPQSVALRIASGALSGSGTLQVRP
                     DTTEYTGSAAVQELRLEEHDSKALLLAWQSAETGTLRVALAPLRIEAGEIAARAPEGR
                     LVIEQDGSVNFAALFAREADKGEPMQASIERLSIEKGTLHFADRSLATPFEATVRELS
                     GTVTGFTTVAGEPARVRLDGRVQPFGQARIRGTIDLDSPAELADIRANFRNLRLQSFN
                     PYIAKFAGYRIESGRLSAELRYVVRDGELRGSNQLVFEQMQLGEKLERRAVVDVPLEL
                     LVALLADPSGRIDLAIPVSGNLHNP"
     gene            complement(1793..2176)
                     /locus_tag="VFX94_07855"
     CDS             complement(1793..2176)
                     /locus_tag="VFX94_07855"
                     /inference="COORDINATES: protein motif:HMM:NF017917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HEX5768132.1"
                     /translation="MPGRKSLPDTLKRSPAKAQRTWIKTHDNAIEQYGEGERAHRTAF
                     AALKHSFQKKGDRWVKKGSRGPSDPRARKGAGEARRGKGETFGGIDYYGSTREELYER
                     AKKLDVPGRSRMSKKDLARAIARKQ"
     gene            2463..>2796
                     /locus_tag="VFX94_07860"
     CDS             2463..>2796
                     /locus_tag="VFX94_07860"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX5768133.1"
                     /translation="MATALQQAVLWIWHTPSAIAAALERDAVHIAMHASLLAAALVFW
                     SAVLRPASGRYWGSIAALLVTFKISGIVCVGFLLQTQAVYEAYRNGVDDDLFFGPDAT
                     QGWFTRFKA"
BASE COUNT          386 a         1000 c          970 g          440 t
ORIGIN      
        1 cgggttgtgg agattgccgc tcaccgggat ggcgagatcg atgcggccgc tcgggtccgc
       61 cagcagcgcc accagaagct cgagcggcac gtcgaccacg gcgcggcgct ccagcttctc
      121 gccgagctgc atctgttcga acacgagctg gttcgagccg cgcagctcgc cgtcgcgcac
      181 gacgtagcgc agctcggccg acagccgccc cgactcgatg cggtagccgg cgaacttggc
      241 gatatacgga ttgaagctct gcagccgcag attgcggaag ttcgcgcgga tatcggcgag
      301 ctcggcgggc gaatcgaggt cgatcgtgcc gcggatccgt gcctggccaa atggctgcac
      361 gcggccgtcg aggcgcacgc gcgcgggctc gccggcgacg gtggtgaagc cggtgacggt
      421 gcctgaaagc tcccggacgg tggcctcgaa cggcgtggcg agcgagcggt cggcgaagtg
      481 cagcgtgccc ttctcgatgc tcaggcgctc gatgctcgcc tgcatgggct cgcctttgtc
      541 cgcctcgcgg gcaaacagcg ccgcgaaatt gacgcttcca tcctgctcga tgacgagtcg
      601 tccctccgga gcgcgcgcgg cgatctcgcc cgcctcgatc cgcagcggcg cgagcgcgac
      661 gcgaagcgtc ccggtttcgg cgctctgcca ggcgagcagg agcgccttgg agtcgtgctc
      721 ctccaggcga agctcctgca ccgcggcgct gccggtgtac tccgtggtgt ccgggcgaac
      781 ctgcagcgtg ccgctcccgg agagcgctcc cgatgcgatg cgcagcgcca cgctctgcgg
      841 gagccagcgc tgcgcctgtg cgagcggcaa ggcatttgcc tcgaccttca tgtccaggcg
      901 cagcggcgat atggccacgc tcccctgcgc cgagagcttg ccctgcggcg cgacggtggc
      961 ctgcgccgtg acctgcacgg gtgattggag cggaagggaa acgctctgcg cggtgaccgt
     1021 cgcctccggc agctcgatcc cggcataagc gagccggttg cccgtgatgg aaacatcgcg
     1081 cagctcaagc tcgcccgatc ggtagacgta gcgcgccgag cccttcagtt gcccatgcgc
     1141 ttccgcaaaa agctgcccgg cgcccagcgc cgcgacggta agcgtacctt cagcagcgag
     1201 cggcgcgagc gccaccgtgc cacgcgaagc gaactgccct gccccgccgg cgaggcgccc
     1261 ggcggcttcg tagttgccgg ccgcggcatc caacgtcgaa aggtcgctcg cgcgcagcgt
     1321 gagcgcctca agcgcgacgt cgcgtgggga gtcgcgcagc accccgccct cgaccgcaag
     1381 ctgccgcacg gttacggccg ccgcgccggc ctcttcgcgt gccttgccga acgcgggaag
     1441 catgcccacc tcgacctgtg gcgcgatcac gcgcacctgc tcgaagatcc agccccggcg
     1501 ccagagtgaa gcccaggcga gatcggccga tgcgctctgc gcctgggcga agacgcggcc
     1561 ctgcgagccg cgcagcgcca cgtcgtccaa ctccacgcgc agctcgaagg gccgggcgcg
     1621 gacgtcggcg acatcgagcg tgagggcgcg ttcggccgct agcgcggcga gggcgtcgcg
     1681 cacgtagccc ggcgcaagcc agtatcccgc cgccgcatac agcccgacga gtaccgccac
     1741 cgcgccgagg gcgacgaggg cggtgcgctt catgcgccgc cgagcgggcg gatcactgct
     1801 tgcgcgcgat ggcgcgcgcc aggtccttct tcgacatgcg cgagcgcccc gggacgtcga
     1861 gcttcttcgc ccgctcgtag agctcctccc tggtgctgcc gtagtagtcg atgccgccga
     1921 aggtctcgcc cttgccgcga cgcgcttcgc ccgcgccttt tctcgcgcgc gggtcggacg
     1981 gcccgcgcga gcccttcttc acccagcggt cgcccttctt ctggaacgaa tgcttcagcg
     2041 cagcgaacgc ggtacggtgc gcgcgctcgc cttcaccgta ctgctcgatg gcgttgtcat
     2101 gcgtcttgat ccaggtgcgc tgcgccttgg cgggcgagcg cttgagagtg tcgggaagac
     2161 tcttacggcc cggcatgcgt gcctcctttg gtagtgcgat ctgcaggcga ccgcccgcaa
     2221 atttcgcgcc gacccggcat ggacattgcg cccggcccag ccatgacgtg gctcgagtaa
     2281 cgggcgtgag ccttccgtgg acttcgccgc cgtgcgccca cgccgaggaa agctcgccgg
     2341 cgcatggaca ctcgatccat tggtcctcgc cccgctcgcc gcagcgctgt actggcgcgg
     2401 ctcgcgcgcc tcggcgcggc gcggggttgc gcgcaggctg gcgctggctc tcgggtctcg
     2461 ccgtggccac ggcgctgcag caggcggtgc tctggatctg gcatacgccg agcgcgatcg
     2521 ccgccgcgct cgagcgtgac gccgtgcaca tcgccatgca cgcgagcctg ctcgccgccg
     2581 cgctcgtctt ttggagcgcg gtgcttcggc cggcaagcgg caggtactgg ggctcgatcg
     2641 ccgcgctgct cgtcacgttc aagatctcgg gcatcgtgtg cgtcggcttt cttctgcaga
     2701 cccaggcggt ctacgaggct taccgcaacg gcgtggatga cgatctcttc ttcggcccgg
     2761 acgctacgca gggctggttc acccgcttca aggcgg
//
LOCUS       DASUNT010000389         3759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1195341, whole genome shotgun sequence.
ACCESSION   DASUNT010000389 DASUNT010000000
VERSION     DASUNT010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195341"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VFX94_07865"
     CDS             complement(<1..365)
                     /locus_tag="VFX94_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX5768134.1"
                     /translation="MKQTLTLLIALLAFAGGASAQIYRWVDEKGTVHYSNSAPPNGVK
                     ATVFDREAKAGPPSPDSAECHTVRCQGERLEQRLARQQELDARLAAERAAATPPRPRG
                     LDFRSYISLNRGMSEGELLV"
     gene            complement(362..1306)
                     /locus_tag="VFX94_07870"
     CDS             complement(362..1306)
                     /locus_tag="VFX94_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019452122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEX5768135.1"
                     /translation="MKYTIQITPPDIKRYRAANTGVDYVHLMDCGKPGPNVLVQALTH
                     GNEFSGAIALDFLFREEIQPRRGRLILAFANVAAFERFDFADPDRARCIDEDYNRVWA
                     DSVLLGPRDSVELRRARELRPFVDAADYLLDLHTMTEPCRPIMVCGMLDKGAQFARRV
                     GVPADLLIDTGHPAGLRMRDRGGFGDPTSPKNALLIEAGQHWEKSSVDVAIDTTLRFL
                     HVTGVVDEAFVRPRLRMTSPAKQRLVRVTEPVTALSAGFRFARPWKGLEVVPKAGTLI
                     ATDGDKAWHTPYDDCVMVMPSMAHVKPGNTMLRLGRYE"
     gene            complement(1303..2097)
                     /locus_tag="VFX94_07875"
     CDS             complement(1303..2097)
                     /locus_tag="VFX94_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX5768136.1"
                     /translation="MAPLVLDSPHSGHDFPPDFDAIVSESELRESEDCYVDELYAAAH
                     ELGVPLLAARFPRTYLDPNRNAGDVDLDLIEGKWPWEYRPSGKARIGKSLIWRTLEDG
                     RPIYARKLAPEAVRRRIDRVHAPYHRALQELLDKAFTTHGRVYHVNCHSMRSVAGKQS
                     EDGPGSVRPDFVLGDRDGSTCEPRFTELVRETLAGMGYRVTVNDPYKGVELIRAYSHP
                     ARRRHSLQIEVNKRLYMDERTLQKTPGFRKLQNDLAELLKVLLQTL"
     gene            2115..2777
                     /locus_tag="VFX94_07880"
     CDS             2115..2777
                     /locus_tag="VFX94_07880"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5768137.1"
                     /translation="METYYARRAAEYEKIYQKPERQADLARLRRDIPALFRGERVLEI
                     ACGTGYWTPLIAELAGSVLAVDYNDETLAIARTKEYPKRNVRFRRADAYALPDFEETF
                     SSSFAGFWWSHVPVSKLAAFIRGLHARLQPGARVAFLDNRYVEGSSTAISRTDAEGNT
                     YQCRRLADGSAHEVLKNFPTGPQLRERLAPFGTEVAFQQYEYYWLVTYRIMAATYPGG
                     KP"
     gene            2774..>3759
                     /locus_tag="VFX94_07885"
     CDS             2774..>3759
                     /locus_tag="VFX94_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5768138.1"
                     /translation="MIRLLLAALFAFTSLAAAQELRIGLASEPTAMDPHYHNLSPNNS
                     LLSHIFESLVGQDERQRLTPDLAESWKAIDPTTWEFRLRKNVRFHDGTPFTADDVIFS
                     FERAPNVEGSPSSFGIYARGKTFVKVDDHTLHVKTAAPYPLMPNDVSQVFIVSRKHGQ
                     GAKTPDYNSGKAAIGTGPYRFVEYTPGNRIVMQRNEQYWGDKPVWQRVIFRGIKSDPS
                     RVAALLAGDVDMIDEVPAVDMERLKKDPKLTIAQTVSNRIIYLHLDHFRDDSPFVRAK
                     DGGAIKNPLRDVRVRKAISMAIDRDAIVSRVMEGQAIKASQLLPEGFFGVSS"
BASE COUNT          625 a         1260 c         1238 g          636 t
ORIGIN      
        1 acgaggagct cgccttccga catcccgcgg ttgagcgaga tgtagctgcg gaaatccagg
       61 ccgcgcgggc gggggggcgt cgcggcggcg cgctcggcgg caaggcgtgc gtcgagctcc
      121 tgctggcgcg cgagccgctg ctcgagccgc tccccctggc agcgcaccgt gtggcactcg
      181 gcgctgtccg gcgagggagg cccggccttc gcttcgcgat cgaacaccgt cgccttcacg
      241 ccgttgggcg gcgctgaatt ggagtagtgc accgtgccct tctcgtccac ccagcgatag
      301 atctgcgcgg acgcgccgcc ggcgaacgca agcagggcaa tgaggagcgt aagggtttgt
      361 ttcactcgta gcggcccagg cgcagcatcg tattccccgg cttcacgtgc gccatggacg
      421 gcatcaccat gacgcagtcg tcgtaggggg tatgccacgc cttgtcgccg tcggtggcga
      481 tcagcgtgcc ggccttcggc accacttcga gccctttcca gggccgcgca aaccggaagc
      541 cggcgctgag cgcggtgacc ggctcggtga cacggaccag gcgctgcttc gcgggggacg
      601 tcatgcgcaa gcgcggcctc acgaaggctt cgtccacgac acccgtgacg tgcaggaacc
      661 gcagcgtcgt gtcgatcgcg acgtcgaccg agcttttctc ccaatgctgc ccggcttcga
      721 tgagcagcgc gttcttcggg ctcgtcgggt cgccgaaccc gccgcggtcg cgcatgcgca
      781 gccccgccgg gtgcccggtg tcgatcagca gatcggccgg aacaccgacg cggcgcgcga
      841 actgcgcgcc cttgtccagc atgccgcata ccatgatcgg ccggcacggc tcggtcatcg
      901 tatggaggtc gagcagatag tcggccgcgt ccacgaacgg ccgcagctcg cgcgcgcgcc
      961 tcagctccac cgagtcgcgc gggccgagca acaccgagtc ggcccacacc cggttgtagt
     1021 cctcgtcgat gcaacgcgcg cgatccgggt cggcgaaatc gaagcgctcg aacgcggcca
     1081 cattggcgaa ggcgaggatc agcctgcctc tgcgaggctg aatttcttcc ctgaacagaa
     1141 aatcgagcgc aatcgcgccg gaaaattcgt tcccgtgcgt gagcgcctgc accaggacgt
     1201 tcggcccggg cttgccgcaa tccatgaggt gcacatagtc cacgccggtg tttgccgccc
     1261 ggtagcgctt gatgtcgggc ggcgtgatct ggatcgtgta cttcacagcg tctggagcag
     1321 caccttgagc agctccgcga gatcgttctg cagcttcctg aaccccggtg ttttctgcag
     1381 ggtgcgctcg tccatgtaga ggcgtttatt gacctcgatc tgcaggctgt gccggcgtct
     1441 cgctgggtgc gagtatgcgc ggataagctc cactcccttg tatgggtcgt tcaccgtcac
     1501 ccggtagccc atgccggcga gcgtttcgcg cacgagctcg gtgaagcgcg gctcgcaggt
     1561 gctgccgtcg cggtccccga gcacgaagtc cggacgcacg ctgcccgggc cgtcctcgct
     1621 ctgcttgcct gcgaccgagc gcatcgaatg gcaattgacg tggtacaccc ggccgtgcgt
     1681 tgtgaacgcc ttgtcgagca gctcctggag cgcgcggtgg tacggcgcgt ggacgcgatc
     1741 gatgcgccgg cgtaccgctt ccggggcgag cttcctcgcg tagatgggcc ggccgtcctc
     1801 gagggtgcgc cagatgagcg acttgccgat gcgcgccttg ccgctcggcc ggtattccca
     1861 cggccactta ccctcgatga ggtcgaggtc gacgtcgccc gcgttgcggt tcggatcgag
     1921 gtaggtgcgg ggaaagcgtg cggcgagcag tggcacgccg agctcgtgcg ccgcagcgta
     1981 gagctcgtcc acgtagcagt cctccgactc gcgcagctcg ctctcgctca cgatggcgtc
     2041 gaagtcgggc ggaaagtcgt ggcccgagtg cggagaatcg agaacgagcg gcgccatcgc
     2101 tgtagcatac ttcagtggaa acctattacg cgcgccgcgc cgccgaatac gagaagatct
     2161 accaaaaacc ggagcgccag gcagacctcg cgcggcttcg ccgcgatatc ccggcccttt
     2221 tccggggcga gcgggtgctc gagatcgcgt gcggcaccgg ttactggacg ccgcttattg
     2281 cggagctggc cggctcggtg ctcgccgtcg actacaacga cgagacgctg gcgatcgccc
     2341 gaaccaagga gtatccgaaa cgcaatgtgc gcttccggcg cgccgatgcc tacgcgctgc
     2401 cggacttcga ggaaacgttc tcgtcctctt tcgccggctt ctggtggtcc catgtcccgg
     2461 tgtcgaagct tgcggccttc attcgaggct tgcatgcaag gctgcagccg ggcgcgcggg
     2521 tggcgttcct ggataaccgc tatgtcgagg gctccagcac ggccatttcg cggaccgacg
     2581 cggagggaaa cacctaccag tgccgtcgcc tcgccgacgg ctctgcccac gaggtgctga
     2641 agaacttccc caccgggccg cagctccgcg agcgcctggc acccttcggc acagaggtgg
     2701 cattccagca gtacgagtac tactggctcg tgacctatcg cataatggcc gcgacctatc
     2761 cgggaggaaa accatgatcc gcttgctgct tgccgctctg ttcgccttca cgagcctggc
     2821 cgcggcccag gagctgcgca tcggccttgc gtccgagccg acggcgatgg acccgcacta
     2881 ccacaatctc tcgccgaaca actcgcttct cagccacatc ttcgagagcc tggtaggaca
     2941 ggacgagcgc cagcggctca cgccggacct cgccgagtcg tggaaggcga tcgacccgac
     3001 gacctgggag ttcaggctgc gcaagaacgt ccgcttccac gacggcacgc cgttcaccgc
     3061 ggacgacgtg atcttcagct tcgagcgcgc gccgaacgtc gagggcagcc cgtcgagctt
     3121 cggcatctac gcgcgcggca agaccttcgt gaaggtcgat gaccacacgc tgcacgtcaa
     3181 gacggcggcg ccctatccgc tgatgccgaa cgacgtgtcc caggtcttca tcgtctcgcg
     3241 caagcacggc cagggcgcga agacgcccga ctacaactcg ggcaaggccg cgatcggcac
     3301 cggcccgtat cgcttcgtcg agtacacgcc gggcaaccgc atcgtgatgc agagaaacga
     3361 gcagtactgg ggcgacaagc ccgtttggca gcgcgtgatt ttccgcggca tcaagtccga
     3421 tccctcgcgc gtcgcggcgc tgctcgcggg cgacgtcgac atgattgacg aggtgccggc
     3481 agtggacatg gagcggctca agaaagaccc gaagctcacg atcgcgcaga ccgtctcgaa
     3541 ccgcatcatc tacctgcacc tcgatcattt ccgcgacgat tcgcctttcg tgcgggcgaa
     3601 ggacggcggc gcgatcaaga accccctgcg cgacgtgcgc gtgcgcaagg cgatttccat
     3661 ggcgatcgac cgcgacgcca tcgtgagccg ggtgatggaa ggccaggcga tcaaggccag
     3721 ccagctcctg ccggaaggct tcttcggcgt gagcagcaa
//
LOCUS       DASUNT010000390         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_581065, whole genome shotgun sequence.
ACCESSION   DASUNT010000390 DASUNT010000000
VERSION     DASUNT010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581065"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..549
                     /locus_tag="VFX94_07890"
     CDS             64..549
                     /locus_tag="VFX94_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX5768139.1"
                     /translation="MPSFDIVSQVDRQEVKNAVEQTNKEISNRFDFKGSDARVEQTDL
                     ALTVYADDEFKLGQVLDVLRQRLARRNVDVRSLEPGAVEKISGDKVKRPVSIKVGVPQ
                     EKAKHIQKLIKDSKLKVAASIQGDAVRVSGAKKDDLQAAIQLVKKSVIDLPLQYINFR
                     D"
     gene            complement(533..1237)
                     /locus_tag="VFX94_07895"
     CDS             complement(533..1237)
                     /locus_tag="VFX94_07895"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEX5768140.1"
                     /translation="MAILRTARTEGPGYFATYLERRSIPWQLIPLDEGDKVPADPRAY
                     SGLAVMGGPMSANDDLPWIPPLLELLRDAVRRDVPLIGHCLGGQLMSCAFGGAVRPNP
                     VREIGWGEVRVADNGVAREWLGELQAFETFQWHGETFSIPPGATRVLEGEHCLNQAFA
                     LGRHFAMQCHVEMTPELVRTWLSTGGEEIRSHRKSPAVQRAAEIEKDLEPRLERLHQI
                     ADRIYDRWSEGLSRGS"
     gene            1278..1880
                     /locus_tag="VFX94_07900"
     CDS             1278..1880
                     /locus_tag="VFX94_07900"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEX5768141.1"
                     /translation="MSTAVGATDIALIGVIGDKAAVLALDGGDPRTVKVGQKWSGITV
                     ISVERGEATVEIEGKRRVLKMGQHYRTAAAAADERQSVTLAADSRGHFITQGAINGNP
                     VRFLVDTGATVVALPAAEAVRLGIDYRKGQPGMSSTAGGVVPTYRVRFDTVRLGEIEV
                     NGVDGVVLEQGLDIALLGMSFLNRLEMKRDGHTMVLIRRF"
BASE COUNT          362 a          663 c          668 g          326 t
ORIGIN      
        1 gcccatcgag cggcagctgc ggcgcctgtg gacgcagatt tccccccgat aagcgagacc
       61 cgcatgccgt ccttcgacat cgtttcccag gtcgaccgcc aggaagtgaa gaacgcggtc
      121 gagcagacca acaaggaaat ctccaaccgc ttcgacttca agggctccga cgcccgcgtg
      181 gagcagacgg acctcgcgct taccgtatat gccgacgacg agttcaagct cggccaggtg
      241 ctcgacgtgc tgcgccagcg ccttgccagg cgcaacgtcg acgtgcgctc gctcgagccg
      301 ggggcggtcg agaagatctc gggcgacaag gtgaagcgcc cggtcagcat caaggtcggc
      361 gtgccccagg aaaaggcgaa gcacatccag aagctcatca aggattcgaa gctgaaggtc
      421 gccgcctcga tccagggcga cgcggtgcgc gtttcaggcg cgaagaaaga cgacctgcag
      481 gcggcgattc agctcgtgaa gaagtcggtc atcgaccttc cccttcagta catcaacttc
      541 cgcgactgag cccctcgctc cagcggtcgt aaattcggtc cgcgatctgg tgcaggcgtt
      601 ccaggcgcgg ctccaggtcc ttctcgatct ccgccgcacg ctgtacggcg gggcttttcc
      661 ggtggctgcg aatctcctcg ccgccggtgc tcagccacgt gcgcaccagc tcaggcgtca
      721 tctcgacgtg gcattgcatg gcgaagtggc gcccgagcgc gaatgcctgg tttaggcaat
      781 gctcgccctc gagcacgcgc gttgctcccg gcgggatgct gaaggtctcg ccgtgccact
      841 ggaacgtctc gaaggcctgc agctctccga gccactcgcg cgccacgccg ttatcggcga
      901 cgcgtacctc gccccagccg atctcgcgca ccgggttcgg gcgcaccgcg ccgccgaaag
      961 cgcatgacat gagctgcccg ccaaggcagt ggccgatgag cggaacatcg cggcgcaccg
     1021 cgtcgcgcag cagctcaagc agcggcggga tccagggcag gtcgtcgttc gcgctcatcg
     1081 gcccgcccat gacggcgagg ccggaatagg cgcgcgggtc ggcgggtact ttgtcacctt
     1141 cgtccagggg aatgagctgc cagggaatcg agcgacgctc gaggtaagtg gcaaaatatc
     1201 ctggaccttc cgtacgagcg gttctaagaa tagccacggg cttcatgcgc accgctattt
     1261 tgcttctact ttcgtccttg tccacggcag tgggcgcgac cgatatcgcg ctgatcggcg
     1321 tgatcggcga caaggccgca gtcctggcgc tggacggcgg cgaccccagg acggtcaagg
     1381 tcgggcagaa gtggagcggc atcacggtga tttcggtcga gcgcggcgaa gccacggtgg
     1441 agatcgaggg caagcgccgg gtgctgaaga tgggccagca ctaccgcacc gcggccgcgg
     1501 cggccgacga gcgccagagc gtgacactgg ccgcggactc gcgcgggcac ttcatcacgc
     1561 agggcgcgat caacggcaac cctgtgcgct tcctcgtcga caccggcgcc acggtggtcg
     1621 cgctgccggc ggcggaggcc gtacggctcg gcatcgacta ccgtaagggc cagccgggca
     1681 tgagcagcac cgcgggcggc gtagtgccga cataccgggt gcggttcgac accgtgcgcc
     1741 tcggcgagat cgaggttaac ggcgtcgacg gcgtggttct cgagcagggg ctcgacatcg
     1801 cgctgctcgg aatgagcttt ctcaaccggc tcgagatgaa gcgcgacggg cacacgatgg
     1861 tgctcatccg ccgcttctga aattagggga cggagcactt tagggctccg tccccgaatt
     1921 cccccgctta tttcccgggc accagcggca cgaaacgaac cggcaagacg cgcttttcct
     1981 cgaccccgcc gtcggcgcgt ttcgtcagaa gcttcagat
//
LOCUS       DASUNT010000391         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1195592, whole genome shotgun sequence.
ACCESSION   DASUNT010000391 DASUNT010000000
VERSION     DASUNT010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195592"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VFX94_07905"
     CDS             complement(<1..365)
                     /locus_tag="VFX94_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5768142.1"
                     /translation="MLTADRALSLLVVEDHAEVRVALRDWLLASPQAFNVREARSLEE
                     ALLCAAQGPLDLVLVNLELPGPNGLEATRALRKQLPHCRVIVMSVNDSEALRTAAIEA
                     GALAFVSKREMPHALLPLLG"
     gene            complement(425..1075)
                     /locus_tag="VFX94_07910"
     CDS             complement(425..1075)
                     /locus_tag="VFX94_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5768143.1"
                     /translation="MLIADDHAVVAEGLRHLVEAERDIEVVACAVDGREAVQQARETQ
                     PDVVLMDLSMPELNGADATRAILERDPRCRVIVLSMYAQREYVRRALKAGAAGYLVKR
                     SAAKELVEAIRAVHAGQRYLSPRVADVVLEDYTDGRQDDPLARLSAREREVLQLLAEG
                     RTGAQIAERLSLSQKTVETYRARLVEKLGIRDLAGLVRFAIQKGLVSLDEGPSGKS"
     gene            complement(1091..>2943)
                     /locus_tag="VFX94_07915"
     CDS             complement(1091..>2943)
                     /locus_tag="VFX94_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5768144.1"
                     /translation="GAAMRFVVQVEDITESRAVQARLRESERRFRLLVSSVTDYAIYV
                     LSPQGEISSWNTGAERIKGYCEREVLGRHFSIFFTAEERALGKPQRALDTVRASGRFA
                     DEGWRLRKDGSRFWALAALEAMRDEAGTLIGIVNVTRDLSERRTAEERMRQAQALIEA
                     FTDNSPSAMSLKDRDGRYRFANLRFLARHGLRREQVIGRHDAEIFSARQAAVMSLHDA
                     EVLMRGEPVQYEERVEIAGTKYVSLVVKFAAAGGVGMIAGDITDRRLTEQALREQRAL
                     LAEAQKLAGLGWWEWSPGSGQVAWSDQLYRIYGLSPDTFKPGFDAYLERVHPEDREHA
                     AALMARALMDGRGFTKHERIVRPGGEVRYLRTQVEVVRDEAGKPIKVLGASLDFTEQR
                     HSETALRQATHDLHGLTRRLVQAEEAERKRIARELHDRVGQALSALNINLDIVLRSAE
                     LAPGARQRLADSVSLVDSTLQSIEGVMAELRPPLLDEYGLAAALAWHAEEFSRRTGIR
                     VSIDDEAAEAVRSLRLEAALALFRIAQEALTNVLKHARAKAVRVEVALAGDEVVMSIQ
                     DDGAGFDASRAPRGRLGMTTMRERAEAAGGRLEIDSAPGRSTRVVASVPL"
BASE COUNT          431 a         1076 c          983 g          453 t
ORIGIN      
        1 ccgaggagcg gcagcagcgc gtgcggcatc tcgcgcttgg agacgaacgc gagcgcgccc
       61 gcctcgatcg ccgcggtgcg cagcgcttcc gaatcgttca ccgacatcac gatcacacgg
      121 cagtgcggca gctgcttgcg cagcgcgcgc gtcgcctcca acccgttcgg accggggagc
      181 tcgagattca cgagcacgag gtcgagcgga ccctgcgccg cgcacaatag cgcctcttca
      241 aggctgcgcg cctcgcgcac gttgaacgcc tgtggcgaag cgagcagcca gtcgcgcagc
      301 gcgacgcgca cttcggcgtg atcctcgact accagcagcg aaagagcgcg atccgcggtg
      361 agcacgcggc gaaatctagc caatccgcgg ggcgcgcgaa gtcgggagcg ccgtacagaa
      421 ggcgtcagga tttcccggac ggcccttcat ccagagagac gaggcctttc tggatcgcga
      481 agcgcacaag ccccgcgagg tcgcggatgc cgagcttctc cacgaggcgc gcgcggtaag
      541 tctcgaccgt cttttgcgaa agtgagagcc gctcggcgat ctgcgcgccg gtgcgtccct
      601 cggcgaggag ttgcagcacc tcgcgctcgc gcgccgagag ccgcgcgagc gggtcgtcct
      661 gccgcccgtc ggtatagtcc tccagaacta cgtcggctac ccgcgggctg aggtagcgct
      721 gcccggcgtg caccgcgcga atggcctcca ccagttcctt cgccgcggag cgcttcacca
      781 agtagccggc cgccccggcc ttgagtgcgc ggcgaacgta ttcgcgctgc gcgtacatcg
      841 agagcacgat gacccggcag cgcggatcgc gctcgaggat cgcgcgcgtg gcgtcggcgc
      901 cgttcaactc gggcatcgag agatccatga gcacgacgtc gggctgcgtt tcgcgcgcct
      961 gctgcacggc ctcgcggccg tcgaccgcgc acgcgaccac ctcgatgtcg cgctcggctt
     1021 ccaccaggtg gcgcagccct tccgccacca cggcatgatc atcggcgatg agcacgcgaa
     1081 tgggcatctt ctataacgga accgaggcaa cgacgcgggt gctgcgcccg ggcgcggagt
     1141 cgatctcgag cctcccgcca gccgcttcgg cgcgctcgcg catcgtggtc atgccgagcc
     1201 tcccgcgcgg cgcgcgggag gcgtcgaagc ccgcgccgtc gtcctggatg ctcatcacca
     1261 cctcgtcgcc ggcgagcgcc acttccacgc gcaccgcttt cgcgcgcgcg tgcttcagca
     1321 cgttcgtcag cgcctcctgc gcgatgcgga agagcgcgag cgcggcctcg agccgcaggc
     1381 tccgcaccgc ctcggccgcc tcgtcgtcga tgcttacgcg gattcccgtg cgccgcgaaa
     1441 actcctccgc atgccaggcg agggccgccg caagcccgta ctcgtcgaga agcggcgggc
     1501 gcagctccgc catcactccc tcgatcgact gcaacgtgct gtccaccagg ctcaccgagt
     1561 cggcaagcct ttgccttgcc cccggcgcga gctctgcgct gcgcagcacg atgtcgagat
     1621 tgatgttgag cgcggaaagc gcctggccga ctcggtcgtg cagctcgcgc gcgatgcgct
     1681 tgcgctccgc ctcctcggcc tgcaccaggc ggcgtgtcag gccgtgcagg tcgtgcgtcg
     1741 cctggcgcag cgccgtctcg gagtggcgct gctcggtgaa gtcgaggctc gcgccgagca
     1801 ccttgatcgg cttgccggct tcgtcgcgca ccacctcgac ctgcgtgcgc aggtagcgca
     1861 cctccccgcc cgggcgaacg atgcgctcat gcttggtgaa gccgcggcca tccatcagcg
     1921 cgcgggccat cagggcggcg gcatgctcgc ggtcctcggg atgcacgcgc tcgagatacg
     1981 cgtcgaagcc gggcttgaac gtatccggcg agaggccata aatgcgatag agctggtcgg
     2041 accatgcgac ctggccgctc ccggggctcc actcccacca gccgagcccg gcgagcttct
     2101 gcgcctccgc gagcagcgcg cgctgctcgc gcagcgcctg ctcggtgagg cgccggtcgg
     2161 tgatgtcgcc cgcgatcatg ccgactccgc cggcggcggc gaacttcacc accaggctga
     2221 cgtacttcgt tcccgcgatt tccaccctct cctcgtactg caccggctcg ccgcgcatga
     2281 gcacctcggc gtcgtgcagg ctcatcacgg cggcctgccg agccgagaag atctccgcgt
     2341 cgtggcggcc gatgacctgc tcgcggcgca gcccatgccg cgcaaggaag cgcaggttcg
     2401 cgaagcggta gcgcccgtcg cggtccttca agctcatggc cgaggggctg ttgtcggtga
     2461 aggcttcgat cagcgcctgc gcctggcgca tccgctcctc ggccgtgcgc cgctcgctca
     2521 agtcgcgcgt cacgttcacg atgccgatga gcgtacctgc ctcgtcgcgc atcgcctcga
     2581 gcgccgcgag cgcccagaac cggctgccgt ccttgcgcag gcgccagcct tcgtcggcga
     2641 agcggccgct cgcgcgcacg gtgtcgagcg cgcgctgcgg cttgcccaag gcgcgttctt
     2701 ccgcggtgaa gaagatcgag aaatgccggc cgaggacctc gcgctcgcag tagcccttga
     2761 tgcgctcggc gcccgtgttc cagctcgaga tctcgccctg cggcgagagc acgtagatcg
     2821 cgtagtcggt gacgctcgag acaaggaggc ggaagcgccg ctcgctttcg cgcaggcgcg
     2881 cttgcactgc gcgcgactcg gtgatgtcct ccacctggac gacgaaacgc atcgccgccc
     2941 cct
//
LOCUS       DASUNT010000392         3424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1284147, whole genome shotgun sequence.
ACCESSION   DASUNT010000392 DASUNT010000000
VERSION     DASUNT010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284147"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..680
                     /locus_tag="VFX94_07920"
     CDS             69..680
                     /locus_tag="VFX94_07920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX5768145.1"
                     /translation="MEGRHEVERVYGLAAELGIVGPIPPLEVVPDPILVAQARQAFAR
                     LREHALRVAVQISARRPAQRWPLEHFAALIGHLNALGAAPMLLWAPGPRDHPRHPGDD
                     DVAAALMQRLGDKAVAFRTGTLPELIGALAACDAVVTSDGGAMHLAAALRKPIVCFFG
                     DVSPEQWRPWGVPHRALRAASRRAADISVEETLAAFRDLMQLA"
     gene            complement(663..1880)
                     /locus_tag="VFX94_07925"
     CDS             complement(663..1880)
                     /locus_tag="VFX94_07925"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEX5768146.1"
                     /translation="MRALYTVVLRLALPLILVRLWWRGRREPGYRAHIEERFGIYAGE
                     KPAKALWVHAVSVGEARAAAPLVRELQRLAPDHTLVMTCTTAAGRETLGQVYGDSVRA
                     TFLPYDYPETVEAFLERFRPRLGVLMETELWPNLLAACQRRGVPVILANARMSEKSAR
                     GYRRWRSLAVPAIRSLAAVCAQSDADAARLRELGAPRVEVTGNLKFDVTLDEAQLERG
                     RNWKSRVARRVLLLASTRDGEEKLLLDAIGDADFLTVVVPRHPQRFAEVASLAHARKS
                     ATPVPAPAHRVFLGDTMGEMAFYYAAADVAIIGGSFMPLGGQNLIEALAAGVPVVLGP
                     SMYNFAEATRLALEAGAAVQAPDAAAAVREAVALLADAPRRAEMAAAGQKLCLAHRGA
                     TAKHLEVLLGKLH"
     gene            complement(1877..2794)
                     /gene="waaC"
                     /locus_tag="VFX94_07930"
     CDS             complement(1877..2794)
                     /gene="waaC"
                     /locus_tag="VFX94_07930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEX5768147.1"
                     /translation="MTTILFVKTSSLGDVVHNCPAVSDAARALPSAAIDWVVEEPFAA
                     IARMHRSVRRVIPVAVRRWRSRLWRRQVWREIGACKRELTAQRYDAVVDTQSLFKSAL
                     IARAACGTRHGLDRASAREWLAASFYDVRHAVPRDLHAVERNRRLAASALGFPLSGPP
                     EYGFQVPAAPRSGDVVFLTMTSRADKLWPEERWIELARGMRAPVVLPWGSEEERMRAQ
                     RIADAVGGTVLPRQSLEELAKVFVGARAVVGVDTGLTHLAAAVGAPTVGIYCASDPAR
                     TGIYGAPRAHNVGAHGRAPDVVEVLRLLE"
     gene            2836..>3424
                     /locus_tag="VFX94_07935"
     CDS             2836..>3424
                     /locus_tag="VFX94_07935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5768148.1"
                     /translation="MSSAARAPLSAVLITRNAAAVLEPCLDSLAFSDEIVVVDSHSSD
                     DTVELARRKGARVLQKEWLGFGRQKQFAVEQASHDWVLCLDADERVSPELARSIETAL
                     IAPVSPVYRMARRNRFLGRWLAHGEGYPDWSPRLFNRMNARWSDDLVHEKVLFAVTPG
                     TLRGDLMHDSFDDLAAYLERQNRYTTLAARQAYEQG"
BASE COUNT          537 a         1204 c         1153 g          530 t
ORIGIN      
        1 cgctgcgcct tgcgcgtctg ctcgccgccc ggcgcatcgt gtctccttcg ccaaagctcg
       61 tgcgcgagat ggaaggtcgg cacgaggtcg agcgcgtcta cggcctggcg gcggaactcg
      121 gcatcgtagg gcccatacct ccgctggaag tcgtcccaga cccgattctc gttgcgcaag
      181 ctcgccaggc gttcgctcgc ctgcgcgagc atgcattacg ggttgccgtg cagatcagcg
      241 cgcgccgccc tgctcagcgg tggccgctcg agcacttcgc cgcgttgatc ggacacctca
      301 acgccctggg ggcagcaccg atgctgctgt gggcgccggg ccctcgggat caccctcgcc
      361 acccgggaga cgatgatgtg gctgccgcgc tcatgcagcg gctcggcgac aaggcggtgg
      421 cctttcgtac cggcacgctg cccgaactga tcggcgcgct ggccgcctgc gacgctgtgg
      481 tcacgagcga cggcggcgcc atgcacctcg ccgccgcgtt gcgcaaaccc atcgtttgct
      541 tttttggcga cgtgtcgcca gagcaatggc gtccttgggg tgtgccgcac cgggcgctgc
      601 gcgccgcaag ccggagagcg gccgacattt cggtcgaaga gacgctggcc gcgtttcgcg
      661 acctaatgca gcttgcctaa gaggacctcg aggtgttttg ctgtcgcacc acggtgcgcg
      721 aggcagagtt tctggcccgc cgccgccatc tccgcccggc gcggcgcatc ggcaagcaat
      781 gccaccgcct cgcgcaccgc cgcagccgcg tcgggcgctt gtacggccgc tcctgcctcg
      841 agcgcgaggc gggtcgcctc ggcgaagttg tacatgctgg gacccagcac gaccgggacg
      901 ccggccgcga gcgcttctat gaggttctgg ccgccgagcg gcatgaagct gccgccgatg
      961 atcgcgacgt cggccgctgc gtaatagaaa gccatttctc ccattgtgtc gccgaggaag
     1021 acgcggtgcg cgggcgcagg caccggcgtg gcgctctttc gggcgtgcgc gagcgaggcg
     1081 acctcggcga agcgctgcgg atggcgcggc accaccacag tgaggaagtc ggcgtcgccg
     1141 atggcgtcca gtagaagctt ctcttcgccg tcccgggtgc tagccaggag cagcacccgg
     1201 cgcgccacgc ggctcttcca gttgcggccg cgctcgagct gcgcctcgtc gagggtgaca
     1261 tcgaacttga ggtttcccgt cacttcgacg cgcggcgcgc cgagctcgcg cagccgcgcc
     1321 gcatcggcgt cgctttgcgc gcacaccgcg gccaggctgc gaatcgctgg caccgcgagc
     1381 gagcgccagc gccgatagcc gcgagctgac ttctccgaca tgcgcgcgtt cgcgaggatg
     1441 accggcacgc cgcggcgttg gcacgcggcg aggaggtttg gccagagctc ggtttccatg
     1501 agcacgccca ggcgcgggcg gaagcgctcg agaaatgcct ccaccgtctc cggatagtcg
     1561 tacgggagga aagtcgcccg caccgaatcg ccgtagacct ggcccaacgt ctcgcgcccg
     1621 gccgctgtgg tgcatgtcat gacgagggtg tgatccggcg cgagccgctg cagctcgcgc
     1681 accaagggcg ccgcggcgcg cgcttcgccg accgagaccg cgtgcaccca gagcgctttc
     1741 gcgggtttct ccccggcgta tatgccaaag cgctcctcaa tgtgcgcgcg gtacccgggc
     1801 tctcgccggc cacgccacca gaggcgcacc aggatgagcg gtagcgcaag gcgaagcacg
     1861 acggtataca gggccctcat tcgaggaggc gcaacacctc gacgacgtcc ggcgcgcgac
     1921 cgtgcgcgcc gacgttatgc gcacgcggcg cgccgtagat accggtgcgc gcgggatcgg
     1981 acgcgcagta gatgcccacg gtgggcgcac cgaccgccgc cgccaagtgc gtgagcccgg
     2041 tgtccacgcc gaccacggcg cgcgcgccga cgaagacctt ggcgagctcc tcgaggcttt
     2101 gtcgcgggag gaccgtgccg ccgaccgcgt cggcgatccg ctgcgcgcgc attcgctcct
     2161 cttcgctgcc ccacggaagg accaccgggg ctcgcatgcc acgcgcaagc tcgatccagc
     2221 gctcttccgg ccagagcttg tcggcgcggc tcgtcatggt gagaaagacg acatcgcccg
     2281 atcgcggggc cgcgggcacc tgaaatccgt actccggcgg gccggaaagc ggaaatccca
     2341 acgccgatgc ggcgaggcgg cgattacgct cgaccgcgtg cagatcgcgc ggcactgcgt
     2401 gccgcacgtc gtagaacgat gccgcgagcc actcgcgcgc gctcgcccga tccagcccat
     2461 gccgcgttcc gcaggctgct ctggcgatga gcgcgctttt gaaaagcgat tgcgtgtcga
     2521 ccacggcgtc atagcgctgc gcggtgagct cgcgcttgca cgccccaatc tcgcgccaca
     2581 cttgacgccg ccaaagcctg gagcgccagc gtcgcacggc gacgggaatc acgcgccgca
     2641 ccgagcggtg cattcgcgcg atcgcggcga aaggctcctc gactacccaa tcgatcgcgg
     2701 cggaaggcag cgcgcgcgcc gcatcgctca ctgcaggaca gttatggacc acgtcgccaa
     2761 gagaggacgt cttgacgaag agtattgttg tcattggata gaatacggcc cgctgattct
     2821 aagggaattg cccatttgag ctccgcggcg cgtgcgcctc tctcggcggt cctcatcacc
     2881 cgcaacgccg ccgcggttct cgagccctgc ctggacagcc tcgccttctc cgacgaaatc
     2941 gtggtcgtcg actcgcattc gagcgacgac accgtggagc tcgcgcggcg caagggagcg
     3001 cgcgtcttgc agaaggaatg gcttggcttc ggccggcaga agcagtttgc ggtcgagcag
     3061 gcgagccacg actgggtcct ctgcctcgac gccgacgagc gcgtctcgcc ggaacttgcg
     3121 cgttcgatcg aaaccgcgct catcgcgccg gtctcacccg tgtaccgaat ggcgaggcgc
     3181 aaccgcttcc tcggccgctg gctcgcgcac ggcgaaggct atccggactg gagcccgcgc
     3241 ctcttcaacc gcatgaatgc acgctggagc gacgatctag tgcacgagaa ggtcctgttc
     3301 gccgtgacgc cgggaacgct gcgcggcgac ctgatgcacg actcgttcga cgaccttgcc
     3361 gcctatcttg agcgccaaaa ccgctatacc acgctcgccg cgcgccaggc ctacgaacag
     3421 ggcc
//
LOCUS       DASUNT010000393         2046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_757023, whole genome shotgun sequence.
ACCESSION   DASUNT010000393 DASUNT010000000
VERSION     DASUNT010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757023"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFX94_07940"
     CDS             complement(<1..194)
                     /locus_tag="VFX94_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5768149.1"
                     /translation="MNLGRENHPELRDAVRALCSQFDGAYWQDLDEKRAYPEAFVEAL
                     TKAGWMSALIPEEYGGSGLSL"
     gene            complement(191..895)
                     /locus_tag="VFX94_07945"
     CDS             complement(191..895)
                     /locus_tag="VFX94_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HEX5768150.1"
                     /translation="MSAIRSDLGWSTPERIVVRGKDLPREILGTLNLGDMAFFELTGR
                     FPDEKQSRLFNAMAVTLVEHGVTPSALAARLTYLGAPEAMQAAVAAGLCGLGSVFVGS
                     MEGCARLLSGGKNAEQIVAENKRIPGLGHPLHKPVDPRTVRLFEIARETGFYGKYCEL
                     IGQIGKMKGLPVNATGAIGALACELGLDWRAVRGIGVMARAVGLVGHLLEESRRPMAE
                     AVWHQVEEQASRHLKP"
     gene            complement(932..1843)
                     /locus_tag="VFX94_07950"
     CDS             complement(932..1843)
                     /locus_tag="VFX94_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5768151.1"
                     /translation="MKALEGKSVVVTGAGGGIGRDFALAMAAAGARVLVNDIGTSVKG
                     EGRDRAPAQLVVNEIRQQGGVAVANTDSVADWESANRIVQSAVDSFGRIDVVVNNAGI
                     LRDRFFFNMSVEEWRAVIDVHLNGSFYVSRAAAPHFKSQNSGCYVHMTSTSGLVGNLG
                     QANYSAAKLGIVGLSKSIALDMAKYNVRSNCISPFAWSRMIGSIPTETDEQRARVEKL
                     KSMETAKIAPLAVFLASDAAKDVNGQVFAVRANEIFLMSQSRPLRSVHRAEGWTGESI
                     ASHAIPALRAHFYPLDRSQDIFSWDPL"
     gene            complement(1840..>2046)
                     /locus_tag="VFX94_07955"
     CDS             complement(1840..>2046)
                     /locus_tag="VFX94_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5768152.1"
                     /translation="FTNLFWLERACKAQVAAMACNTKLHLPAQDVIEKTNHLYRPETR
                     RRWGPLEWPALLRLADRKYPGFRD"
BASE COUNT          353 a          672 c          661 g          360 t
ORIGIN      
        1 agggagaggc cggagccgcc atactcctcg gggatgagcg ccgacatcca gccagccttg
       61 gtcagcgcct cgacgaaggc ttcggggtag gcgcgcttct cgtcgaggtc ctgccagtag
      121 gcgccatcga actgactgca gagcgcgcgc accgcgtcgc gcagctcggg atggttttcg
      181 cgtccgaggt tcatggcttg agatgccggg aagcctgctc ctcgacctga tgccacaccg
      241 cctcggccat cggccggcgc gactcttcga gcaggtggcc gacgaggccg accgcgcgcg
      301 ccatgacgcc gattccccgc accgcgcgcc agtcgaggcc gagctcgcag gcgagggcgc
      361 cgatggcgcc ggttgcgttc accggcaggc ctttcatctt gccgatctga ccgatgagct
      421 cgcagtattt tccatagaac ccggtctcgc gcgcgatctc gaacaggcgc acggtgcgag
      481 ggtccaccgg cttgtggagc ggatgtccaa gtcccgggat acgcttgttc tcggcaacga
      541 tctgctcggc gttctttccc cccgaaagca gccgggcgca tccttccatc gagccgacaa
      601 aaacgctgcc caggccgcaa agcccggccg ccaccgcggc ctgcattgcc tcgggcgccc
      661 cgaggtaggt aaggcgcgcg gcgagcgcgc tcggcgtgac gccatgctcc accagcgtga
      721 cggccatcgc attgaagagc cgcgactgct tttcatcggg gaagcggccg gtcagctcga
      781 aaaatgccat gtcgcccagg ttcagcgtgc cgaggatctc gcgcggcaga tccttgccgc
      841 gcaccacgat gcgctcgggc gtcgaccagc caagatcgct gcgaatggcg ctcatgtcag
      901 ctctttccag tagttagctc tttgacgctc ttcacagcgg atcccaggag aaaatgtcct
      961 gcgagcgatc cagcggatag aaatgagctc tgagcgccgg gatggcgtga gaagcgatcg
     1021 attccccagt ccagccctcc gcgcggtgca ccgagcgcag cgggcggctc tgcgacatga
     1081 ggaagatctc gttcgcgcgc accgcgaaaa cctggccgtt gacgtccttc gcagcgtcgg
     1141 aagcgaggaa cacggcgagc ggtgcgatct tcgcggtttc cattgatttc agcttctcca
     1201 cgcgcgcacg ctgctcatcg gtctcggtcg ggatcgagcc gatcatgcgg ctccaggcaa
     1261 agggcgagat gcagttcgag cgcacgttgt acttcgccat gtcgagcgcg atggacttcg
     1321 aaagccccac gatgccgagc ttcgccgccg aatagtttgc ctggccgaga ttgcccacca
     1381 gcccggaggt ggaagtcatg tgcacataac aaccggaatt ctgcgacttg aagtgcggtg
     1441 cggcggcgcg cgagacatag aacgagccgt tcaggtgcac gtcgatcacc gcacgccatt
     1501 cctcgaccga catgttgaag aagaagcggt cgcgcaggat gccggcgttg ttgacgacga
     1561 cgtcgattct tccaaaagaa tctaccgcgg actgcacgat gcgattggct gattcccaat
     1621 ccgccacgct gtcggtattg gcgaccgcga ccccgccttg ctgtcttatc tcattgacga
     1681 caagctgggc cggagctcga tcgcgccctt cgcctttgac cgaggtgccg atgtcgttca
     1741 ccaggaccct ggcgccggcc gcggccatgg cgagcgcgaa gtcgcggccg atgccgccgc
     1801 ctgcgccggt gacgacgacg ctcttgcctt ccagggcctt caatcgcgga agcccgggta
     1861 cttcctgtcg gcgaggcgca gcagcgccgg ccactcgagc ggaccccagc gcctgcgggt
     1921 ctcgggccgg tagaggtggt tcgtcttctc gatcacgtcc tgggccggca ggtggagctt
     1981 cgtgttgcac gccatcgcgg cgacctgcgc cttgcaggcg cgctcgagcc agaagaggtt
     2041 ggtgaa
//
LOCUS       DASUNT010000394         2306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_144774, whole genome shotgun sequence.
ACCESSION   DASUNT010000394 DASUNT010000000
VERSION     DASUNT010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144774"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFX94_07960"
     CDS             <1..541
                     /locus_tag="VFX94_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HEX5768153.1"
                     /translation="AWSEPEQIAFLINAYNAYTVEKILTRYPDIGSIWDFGKLFGNPF
                     RDEFFALLGRKSSLDGIEHGILRKRYREPRIHYAVNCASIGCPMLREEAYVAGRLDAQ
                     LEGQARRFLSDRSRNRWHGGRLEVSKIFDWYKEDFEPREAYFARYAAILGMPPGATPR
                     LVFLDYDWSLNDSRSSSPR"
     gene            502..1173
                     /locus_tag="VFX94_07965"
     CDS             502..1173
                     /locus_tag="VFX94_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX5768154.1"
                     /translation="MVAQRLSVVVPALNEAAGIRAALEALRPLRARGHEVIMVDGGSS
                     DGTAELARALCDRVLSAPRGRALQMNAGARAASGTALLFLHADTRLPERADEIVFAAL
                     RSARWGRFDVEIEGSHPLLRLVARGMNLRSRLTGIATGDQAIFVRREAFAGFPEIELM
                     EDVAFSKAMKRLGAPASRRERVVTSGRRWESRGVLRTVLLMWRLRLMYFLGARPERLA
                     RIYHR"
     gene            1170..1760
                     /locus_tag="VFX94_07970"
     CDS             1170..1760
                     /locus_tag="VFX94_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX5768155.1"
                     /translation="MIIVFARAPVPGEAKTRLIPRLGAWHAAQLQLRLTLHALRIARA
                     ANCGPVQLHLARRHSRFRRARLQRGADLGARMQHAFCRARGAILIGTDCPVLTARDLR
                     RAARWLCGGYDAVLAPAEDGGYALIGLRKPIPDLFQGIDWGSERVYAQTVKKLAGYRW
                     RALRRVWDLDRPQDLERLRSLRFPLALRQGARQLKR"
     gene            complement(1807..2058)
                     /locus_tag="VFX94_07975"
     CDS             complement(1807..2058)
                     /locus_tag="VFX94_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768156.1"
                     /translation="MRLAGTIALAVALFGCSERPKLERLAPAERNVRDMVKLAQSRGV
                     AVLRIGTPEPGFTVSPPAFYAGSPDSTACPMKEPSSAKC"
     gene            2113..>2306
                     /locus_tag="VFX94_07980"
     CDS             2113..>2306
                     /locus_tag="VFX94_07980"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX5768157.1"
                     /translation="MKLRIRAPKDFWAGLMFIGFGLFFMAWALTHYQMGSAVRMGPAY
                     FPTVLGGLLAFLGAMVLIES"
BASE COUNT          334 a          764 c          803 g          405 t
ORIGIN      
        1 cgcctggagc gagcccgagc agatcgcctt cctcatcaat gcctacaacg cctacacagt
       61 cgagaagatc ctgacgcgct atccggacat cggctccatc tgggacttcg gcaagctctt
      121 cggcaacccc ttccgcgacg agttttttgc gctcctcggg cgaaagagca gcctcgacgg
      181 catcgagcac ggcatcctgc gcaagcgcta ccgcgagccg cgcatccact atgcggtgaa
      241 ctgcgcctcg atcggctgcc cgatgctgcg cgaggaagcg tacgtggccg ggcgcctgga
      301 tgcgcagctc gaagggcagg cgcggcgctt tctttccgac cgctcgcgca accgctggca
      361 tggcggccgc ctggaggtgt cgaagatctt cgactggtac aaggaggact tcgagccccg
      421 ggaagcgtat ttcgcccgct acgcggcgat cctcggcatg ccgccgggtg caaccccgcg
      481 cctcgtcttc ctcgactacg attggtcgct caacgactct cggtcgtcgt ccccgcgctg
      541 aacgaggcgg ccggcatccg cgcggcgctg gaagcgctgc ggccgctgcg cgcgcgcggc
      601 cacgaggtca tcatggtcga cggcggaagc agcgacggca cggccgagct cgcgcgcgcg
      661 ctgtgcgacc gggtgctcag cgctccgcgt ggacgcgcgt tgcagatgaa cgccggggcg
      721 cgcgccgcct cgggcaccgc cttgctgttc cttcatgccg acactcgatt gccggagcgc
      781 gccgacgaga tcgtgtttgc cgctttgcgt tctgcgcgtt ggggaaggtt cgacgtggag
      841 atcgagggaa gccacccgct gctgagactc gtggcgcgcg gcatgaacct gcgctcgcgc
      901 ctcaccggca tcgctaccgg cgatcaggcg atcttcgtgc ggcgcgaggc atttgcaggc
      961 tttcccgaga tcgagctgat ggaggatgtg gcgttctcca aggcgatgaa gcgtctcggg
     1021 gcgcccgcga gccggcgcga gcgggttgtt acttccggac ggcgctggga gtcgcgcggc
     1081 gtgctgcgca ccgtcctcct catgtggcgg ctgcgcttga tgtacttcct cggcgcacgc
     1141 cccgagcgcc tcgcgcgcat ctaccaccga tgatcatcgt attcgcgagg gccccggtgc
     1201 cgggcgaggc gaaaacgcgc ctgatcccgc gcctcggtgc ctggcacgcc gcgcaactgc
     1261 agctgcggct cacgttgcat gcgcttcgca ttgcgcgcgc ggcgaactgc gggccggtgc
     1321 agctgcatct cgcgcgccgg cactcgcgct tcaggcgagc gcggctgcag cgcggcgcgg
     1381 acctcggggc gaggatgcag cacgcttttt gcagggcacg aggggcaatc ctgatcggga
     1441 ccgactgccc ggtccttacc gcgcgcgatc tgcgccgcgc ggcgcgctgg ctgtgcggcg
     1501 gctacgacgc ggtgctcgcc ccggccgaag atggcggtta tgcgctgatc ggactgagga
     1561 agccgattcc cgatctcttc cagggcatcg attggggaag cgagcgcgtt tacgcgcaga
     1621 cggtgaaaaa gcttgccggc tatcgctggc gggcgctccg ccgcgtgtgg gacctcgacc
     1681 ggccgcagga cctggagcgc ctcagatcgc tccgctttcc cttagcgctt cggcaaggcg
     1741 ctcggcaatt gaagcggtag ccgcgcttgt tgggatgggt cggatctgcc ttcaggctgc
     1801 ggtccctcag cacttcgccg atgatggctc cttcataggg caggcggtgc tgtctggcga
     1861 tccggcgtaa aaggccggcg gtgaaacggt aaagcccggc tccggtgtgc cgatgcgcag
     1921 caccgcgacg cctcggctct gcgcgagctt caccatatcg cgcacgtttc tttccgcggg
     1981 cgcgagccgc tcgagcttcg ggcgctcgct gcagccgaag agagcgaccg cgagagcgat
     2041 cgttccggca aggcgcatcg ggggcgcatg ctacacttcg ccgcagaccg cgccagcggc
     2101 ggaggagagg ggatgaaact caggatcagg gctccgaagg atttctgggc cgggctgatg
     2161 ttcatcggct tcggcttgtt cttcatggcc tgggcgctca cgcattacca gatgggcagc
     2221 gcggtgcgga tgggaccggc gtactttccc accgtgctgg gcgggctgct cgcgttcctc
     2281 ggcgcgatgg tgctcatcga atcgtt
//
LOCUS       DASUNT010000395         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1020136, whole genome shotgun sequence.
ACCESSION   DASUNT010000395 DASUNT010000000
VERSION     DASUNT010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020136"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..744
                     /locus_tag="VFX94_07985"
     CDS             235..744
                     /locus_tag="VFX94_07985"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEX5768158.1"
                     /translation="MTTPIHTAPALYAHAIAIEREAVARYSEFAERMSDLGNDAAAEV
                     FATLARMECEHLEELNKRTAGLALPALGNGEYSWLDAAAPETAARELVFRLMTPRHAL
                     EIALAGEKRAAAFFEHVILTADDAMLRALAREMAADEGDHVTMLEKLLERTPSPIVDW
                     ASVYERQTA"
     gene            741..1322
                     /locus_tag="VFX94_07990"
     CDS             741..1322
                     /locus_tag="VFX94_07990"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5768159.1"
                     /translation="MKDAISILKSEHRSISAVLHGLKELARMAQDAKVRPRFQVLRSM
                     LRYMDEYPERLHHPKENDHIFSRLVAREPSARLLVEELQAEHEEGARLIRELERSLLF
                     FEEGWPAGAREFQQAVDAYADFHWKHMRKEEQQLMPLAERALTAEDWKAINAAFAENR
                     DPVAGLQERDFEQLFSRIANLAPAPVGLGASWR"
     gene            1344..1787
                     /locus_tag="VFX94_07995"
     CDS             1344..1787
                     /locus_tag="VFX94_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5768160.1"
                     /translation="MYKHILIPTDGSEVAEKAVAQGIEFAREAKARVTLFTAVPEYEP
                     PSEAQVFARRVVSIADHARESEETAMRILAHPSDLARAAGLQFDTDYEQSNQPWQAIV
                     NAAKRHGCDAIFMASHGRKGLSRLVHGSQTVDVLTHSDIPTLVVR"
     gene            1877..>2292
                     /locus_tag="VFX94_08000"
     CDS             1877..>2292
                     /locus_tag="VFX94_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5768161.1"
                     /translation="MGSTVSRQQVSVVTLSTIAFTVCFAVWMMFAVIGIPIKKMLDLN
                     ETQFGLLAATPVLTGSLIRLPLGMWTDKYGGRIVFFLLMLATVLPIWLIGEATEFWHY
                     LVLGLFVGLAGGSFSVGTPYVARWFPRSQQGFAMGV"
BASE COUNT          388 a          770 c          758 g          376 t
ORIGIN      
        1 ccgcaggctt cgcacgtacc gtagtcgggc gagtgcagcc gctccagcgc agccgccagc
       61 tgggtcggag ccgcgacctg gtcgcgcatc acgtgctcca ggtggtcacg ctgctcgcgg
      121 gtgaagtaac gatagtgcat caggcctcct tttcggatta atcctacggg ggcccctcga
      181 ttgggctttg accaagatca aagccgccct gcccggatgg cgcatcgtgt cgccatgacg
      241 accccgatcc acaccgctcc ggcgttgtac gcgcacgcca tcgccatcga acgcgaagcc
      301 gtcgcacgct actccgaatt cgccgagcgc atgtccgacc tcggaaacga cgccgcggcc
      361 gaggtgttcg cgacactcgc ccggatggag tgcgagcatc tcgaagagct gaataagcga
      421 accgcgggcc tggcactgcc ggcactcggc aacggggaat acagctggct ggacgccgcc
      481 gcccccgaga ccgcggcgcg cgagctcgtg ttccggctga tgacgccgcg ccacgcgctc
      541 gagatcgcgc tcgcgggcga aaagcgcgcc gccgcatttt tcgagcacgt catcctgacc
      601 gcggacgacg ccatgctgcg cgcgctggcg cgcgagatgg cggccgacga gggcgaccac
      661 gtcacgatgc tggagaagct gctggagcgc acgccgagcc cgatcgtcga ctgggcttcg
      721 gtctacgaaa ggcaaaccgc atgaaagacg ccatttcgat tttgaagagc gagcaccgct
      781 cgatctccgc ggtgctgcac gggctgaagg agctcgcgcg catggcgcag gacgccaagg
      841 tgcggccgcg cttccaggtg ctgcgcagca tgctgcgcta catggacgaa taccccgagc
      901 ggctgcacca cccgaaagag aacgaccaca ttttctcgcg cctcgtcgcg cgcgagccca
      961 gcgcacggct tctcgtcgag gagctgcagg ccgagcacga ggaaggcgcg cgcctcatcc
     1021 gcgagctcga gcgctcgctg ctcttcttcg aggagggctg gcccgctggg gcgcgcgagt
     1081 tccagcaggc ggtggacgcc tacgccgatt tccactggaa gcacatgcgc aaggaagagc
     1141 agcagctcat gccgcttgcc gagcgcgcgc tgaccgccga agactggaag gcgatcaacg
     1201 cggcgttcgc ggaaaaccgc gacccggtcg ccggcctgca ggagcgagac ttcgagcagc
     1261 tcttttcccg catcgccaac ctcgcgccgg cgccggtcgg tctcggcgcg agctggcgat
     1321 gacctacctt taggagtggc ctcatgtaca agcacatcct cattcccacc gacggctccg
     1381 aggtcgcgga gaaggccgtc gcccagggca tcgagttcgc gcgcgaggcg aaggcgcgcg
     1441 tgacgctctt caccgccgtg cccgagtacg agccgccgag cgaagcgcag gtgttcgcgc
     1501 gccgcgtggt ctcgatcgcg gaccacgcgc gcgagtcgga ggaaaccgcc atgcgcatcc
     1561 tggcgcatcc ctccgacctg gcgcgcgccg cggggctaca gttcgacacc gattacgaac
     1621 agagcaacca gccctggcag gcgatcgtga acgccgccaa gcgccacggc tgcgacgcga
     1681 tcttcatggc ttcgcacgga cgcaagggac tgtcgcgcct ggttcacggc agccagaccg
     1741 tcgacgtcct cacgcactcg gacattccca cgctcgtcgt gcgctaaagc ggcgattgcg
     1801 cctcggtttg cttgatgtga atcaagcgaa tttgcgtgcg cgtctctaga gttggatcgc
     1861 aaaggagatg cacaagatgg gctctacggt gagccggcag caggtttcgg tcgtgacgct
     1921 gagcacgatc gctttcacgg tgtgcttcgc ggtgtggatg atgttcgcgg tgatcggcat
     1981 cccgatcaag aagatgctcg atctcaacga gacgcagttc gggctgctcg cggcgacgcc
     2041 ggtgctcacc ggctcgctca tccgcctgcc gctcggcatg tggaccgaca agtacggcgg
     2101 ccgcattgtg ttcttcctgc tgatgctcgc cacggtgctg ccgatctggc tgatcggcga
     2161 ggcgaccgag ttctggcact acctcgtgct cggcctgttc gtcgggctgg ccggcggctc
     2221 gttctcggtg gggacgccct acgtcgcgcg gtggttcccc cgctcgcagc agggcttcgc
     2281 gatgggcgtc tt
//
LOCUS       DASUNT010000396         2802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_581853, whole genome shotgun sequence.
ACCESSION   DASUNT010000396 DASUNT010000000
VERSION     DASUNT010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581853"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VFX94_08005"
     CDS             complement(<1..437)
                     /locus_tag="VFX94_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX5768162.1"
                     /translation="MNELRLPGVLPAWFAEIEPGELRSIAQKAERLVALWASDQTTRF
                     CVHVALASRKGLLWLNVALARDHPRYPGIADIFPAAARMQRAAYDLVGVHAEGGADHR
                     KWLRHAAWPAGTFPLRKNFDSGARYESSLDHYPFVRVEGEGVH"
     gene            complement(434..1882)
                     /locus_tag="VFX94_08010"
     CDS             complement(434..1882)
                     /locus_tag="VFX94_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HEX5768163.1"
                     /translation="MEIALLLGMPLAGAALLALFGHRPWAPEMNSVTSLATLAAAAAL
                     TARVVADGPFSAFDEQVFVDPFNVFLVALTSFVAFTTSLFSRPYMRTELLHQRVNSAR
                     LRLYHSMYQLFSFTMLLTLVSNNVGMLWVAMEAATLSTVLLVSLYRTPESLEAAWKYF
                     ILCSVGIAQALFGTILLYFAAEKVLGAGGNALLWTHLHEVRSELEPTVLSLAFVFLLV
                     GYGTKVGLVPLHNWLPDAHAEGPTPISAVLSGLLLNVALYAVVRAKVLVDGALERNFS
                     GGLMMGFGLLSVVVAAFFLSRQKDIKRMFAYSSVEHMGIMTFAFGMGGTVAAFAGLLH
                     MTVHSLTKSAIFFTVGHASQACGTQDMASIRGLIQTSPVIGWGLALASLAILGMPPFG
                     VFTSEFMILTTAMHEHSWATPFLLLALGVAFAAVFGKVQPMVFGETSAQRMPHPPAVV
                     PIFVHLAIVLLLGLYIPPYLAGWYRQAAAFIG"
     gene            complement(1882..2475)
                     /locus_tag="VFX94_08015"
     CDS             complement(1882..2475)
                     /locus_tag="VFX94_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HEX5768164.1"
                     /translation="MLAQRRILTLIHLLAWQGAALAASTLVVAYATGQVHLYFSAALT
                     LVLKVIVIPWVLHRLIDRLYVRWDIETLLNIPTTMLAGIGLAIFAFALATPISEMAST
                     ITRSTLGIALASVLLSFLMMIVRRKAVPQVIGFLAMENGLLFAATSATYGMPLVVELG
                     IALDVLVATLIFGIFFFHIREQFDSLDIRHMEKLKED"
     gene            complement(2525..>2802)
                     /locus_tag="VFX94_08020"
     CDS             complement(2525..>2802)
                     /locus_tag="VFX94_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004554989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HEX5768165.1"
                     /translation="ALVEWAAAIKLVAYMTIGFALFVPWGIAAESWQAIPVALGALAL
                     KLAAAGAGLALIETLLAKMRIFMVPEFLSGAFLLAVLGMLTHFLVRG"
BASE COUNT          564 a          860 c          992 g          386 t
ORIGIN      
        1 tcgtgcacgc cttctccttc gacccggacg aacgggtaat ggtcgagcga gctttcgtac
       61 cgggctccag agtcgaagtt cttgcgcagc gggaaggtgc ccgcgggcca cgccgcatga
      121 cgcagccatt tgcgatggtc cgcgccgcct tcggcatgga cgcccacgag atcgtaggcc
      181 gcgcgctgca tgcgggccgc cgcggggaag atatcggcga tgccggggta gcggggatga
      241 tcgcgcgcga gcgcaacatt gagccagagc agccccttgc gcgaggcgag cgccacatgg
      301 acgcaaaatc gggtcgtctg gtcgcttgcc cacaaggcga cgaggcgctc ggctttttgc
      361 gcgatcgagc gcagctctcc cggctcgatc tcggcgaacc aggcggggag cacgccgggg
      421 aggcgcagct cattcatccg atgaaggccg ccgcctggcg gtaccagccg gcgagatacg
      481 gcggaatgta aagcccgagc agcaacacga tcgcgaggtg cacgaagatc ggcaccaccg
      541 ccggcggatg cggcatgcgc tgcgcgctcg tttccccaaa taccatgggc tgcaccttgc
      601 cgaacaccgc cgcgaaggcg acgccaagag cgaggagcag gaacggcgtc gcccacgaat
      661 gctcgtgcat cgcggtggtg aggatcatga actcgctcgt gaacacgccg aagggcggca
      721 tgccgaggat cgccaggctg gcgagcgcga ggccccagcc gatgaccggg ctggtttgga
      781 tcaggccccg gatcgaggcc atgtcctgcg ttccgcaggc ctgcgaggca tggccgacgg
      841 tgaaaaagat tgcgctcttg gtgagcgagt gcaccgtcat gtgcagcagg cccgcgaaag
      901 ccgcgaccgt gccgcccatg ccgaaagcga acgtcatgat gcccatgtgc tcgaccgagg
      961 agtaggcgaa catgcgcttg atgtccttct ggcgcgaaag gaagaacgcc gccacgacca
     1021 ccgagagcag gccaaacccc atcatcaggc cgcccgagaa gttgcgctcg agcgcgccgt
     1081 cgaccagcac cttggcgcgc accacggcgt agagtgcgac gttgagcagc agcccggaaa
     1141 gcactgccga gatcggcgtc gggccctccg cgtgcgcgtc gggcagccag ttgtgcagcg
     1201 gcacaaggcc cactttcgtg ccgtagccga cgagcaggaa cacgaaggcg agcgacagca
     1261 ccgtgggctc cagctcgctg cgcacttcgt gcaggtgggt ccacaggagc gcattgccgc
     1321 cggcgccgag caccttctcg gcggcgaagt agagcaggat cgtgccgaac agcgcctgcg
     1381 cgatgccgac cgagcacagg atgaagtact tccacgccgc ctccaggctt tctggcgtgc
     1441 gatagagcga cacgaggagc acggtggaga gcgtggccgc ttccatcgcc acccacagca
     1501 tgccgacgtt gttcgacacc agcgtcagta gcatggtgaa gctgaagagc tggtacatgc
     1561 tgtgatagag gcgcagccgc gcgctgttga cgcgctggtg caggagctcg gtacgcatgt
     1621 agggccgcga gaaaagcgac gtggtgaacg cgacgaagga ggtgagggcc accaggaaga
     1681 cgttgaacgg gtcgacgaat acctgctcgt cgaacgcgga gaacgggccg tccgcgacga
     1741 cgcgcgcggt cagcgcggcc gccgcagcga gggtggcgag gctcgtgacg gaattcattt
     1801 ccggggccca cggccgatgc ccgaagagcg cgagcagcgc cgcgccggcg agcggcatgc
     1861 cgaggagaag cgcgatctcc atcaatcctc tttcagcttt tccatgtggc ggatgtccag
     1921 gctgtcgaat tgctcgcgaa tgtggaaaaa gaagatgccg aagatcaagg tggcgacgag
     1981 cacgtcgagc gcgataccga gctccaccac gagcggcatg ccgtaggtgg cgctggtcgc
     2041 ggcgaagagc agcccgttct ccatcgcgag aaagccgatc acctgcggca ccgccttgcg
     2101 gcgcacgatc atcatgagga acgacagcag cacacttgcc aacgcgatgc cgagtgtcga
     2161 gcgggtgatc gtgctcgcca tctcggagat cggggtcgcc agggcgaagg cgaagatggc
     2221 gaggccgatg ccggcgagca tcgtggtggg gatgttgagc agcgtctcga tgtcccagcg
     2281 cacgtatagc cggtcgatca gccggtgcag cacccacggg atgacgatca ccttcagcac
     2341 gagggtgagc gcggcggaaa agtagaggtg cacctgtccg gtggcgtagg cgacgaccag
     2401 ggtggaagcc gcgagcgcgg caccctgcca ggcgagcaga tggatgagcg tgaggatcct
     2461 gcgctgcgcg agcatcgcga aggcaacgag caggagcagc gccgcgagca ggttgacgaa
     2521 ctggctaacc acggacgagg aagtgggtaa gcatgccgag gacggcgaga aggaaagcgc
     2581 ccgacaggaa ttcgggcacc atgaatatgc gcatcttggc gagcagtgtc tcgatcagcg
     2641 cgaggccggc gccggcggcg gccagcttga gcgcgagcgc gccgagcgcg accgggatcg
     2701 cctgccagct ttcggccgcg attccccacg ggacgaagag cgcgaagccg atcgtcatgt
     2761 aagcgacgag cttgatcgcg gcggcccatt ccaccagcgc ga
//
LOCUS       DASUNT010000397         2645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_669905, whole genome shotgun sequence.
ACCESSION   DASUNT010000397 DASUNT010000000
VERSION     DASUNT010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669905"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VFX94_08025"
     CDS             <1..801
                     /locus_tag="VFX94_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5768166.1"
                     /translation="AIIAGADIRSVRMTPGVDFFAELERAIRELTPKPKMLVIGFPSN
                     PTAQCVELGFFERIVSLARQHDILVVHDLAYADIVFDGYKAPSIMQVPGAREVAVEFF
                     TMSKSYNMAGWRIGFMVGNRELVNALARIKSYHDYGTFTPIQVASIVALEGPQDCVEE
                     IRSVYEKRRNVMVKGLHDIGWRVEVPKASMYVWARIPESYAALGSIEFTKRLLEAAHI
                     AVAPGIGFGDYGDEHVRIALIENEHRLRQALRGMRDMFRKDGLLRGAA"
     gene            798..1397
                     /locus_tag="VFX94_08030"
     CDS             798..1397
                     /locus_tag="VFX94_08030"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5768167.1"
                     /translation="MIHPKPAKLEGHGVRLEPLSLEHEKPLIEAAKDGRLWELFFTSV
                     PETQSTRKYIEEALKGQERGDMLPWAVRDLASGAIVGSTRYHDIIAAADRVEIGWTWY
                     AQRVQRSHVNTACKLLLFGYAFESLKCKVVGLRTDNFNFRSQRAIEALGAKKDGVIRH
                     HWPRRDGTIRDSVMYSVLVHEWPDVKRHLEFRLKRNEAN"
     gene            1384..>2645
                     /locus_tag="VFX94_08035"
     CDS             1384..>2645
                     /locus_tag="VFX94_08035"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX5768168.1"
                     /translation="MKPISVGLLGIGTVGRGTWDVLNRNADEIQRRAGRAIRIAVVAD
                     KELEKARSIVKEKASIVADAFSVVRGSEVEIVVELIGGYTVAKDLVLEAIAHGKHVVT
                     ANKALLATHGNEIFAAAQKKGVMVAFEAAVAGGVPIIKALREGLSANRIEWIAGIING
                     TSNFILSEMRDNGIAFAEALKDAQRRGYAEADPTFDIEGVDAAHKLTILSALAFGIPM
                     QLGKCFREGIQKLTRADIGYAEELGYRIKLLGITKKAASGIELRVHPTLVPARRLIAN
                     VEGVMNAILVKGDAVGPTLYYGAGAGAQPTASAVVADLVDVTRLITADPDQRVPHLAF
                     QPDQLSNERVLFIDEIETSYYLRMRVQDKPGVLADITRTLADSSISIEAMVQKEPGEG
                     ERQVDIVLLTNRALEADVNRAIGTIERLP"
BASE COUNT          497 a          846 c          899 g          403 t
ORIGIN      
        1 gcgatcatcg ccggcgccga catccgctcg gtccgcatga cgcccggcgt cgatttcttc
       61 gccgagctcg agcgcgcgat ccgcgagctc acccccaagc cgaagatgct ggtgatcggc
      121 tttccgtcca atcccaccgc gcaatgcgtg gagctcggtt ttttcgaaag aatagtttct
      181 ctggcgaggc agcacgacat cctcgtggtg cacgatctcg cgtacgccga catcgtgttc
      241 gacgggtaca aggcgccctc gatcatgcag gtacccgggg cgcgcgaggt ggcggtcgag
      301 ttcttcacga tgtcgaagag ctacaacatg gccggctggc gcatcgggtt catggtgggc
      361 aaccgcgagc tcgtcaacgc gctcgcgcgc atcaagagct accacgacta cggcactttc
      421 acgcccatcc aggtggcctc catcgtcgcg ctggaggggc cgcaggactg cgtcgaagag
      481 ataagaagcg tttacgaaaa aagacgcaat gtcatggtga aggggctgca cgacatcggc
      541 tggcgggtgg aggtgccgaa ggcctcgatg tacgtctggg cgaggatccc cgagtcctac
      601 gcggcgctcg gctcgatcga gttcaccaag cgcctgctcg aagccgcgca catcgcggtc
      661 gcgcccggca tcggcttcgg cgactacggc gacgagcacg tgcgcatcgc gctgatcgag
      721 aacgagcacc gcctgcgcca ggcgctgcgc ggcatgcgcg acatgttcag aaaggacggg
      781 ctgttgcggg gcgcggcgtg atccacccca aacccgcaaa gctggagggg cacggcgtgc
      841 gcctggagcc gctgtccctg gagcacgaga agcccttgat cgaggccgcg aaggacggca
      901 ggctgtggga gctgttcttt accagcgtgc ccgagacgca aagtaccagg aagtacatcg
      961 aagaggcgct gaagggccag gagcgcggcg acatgctgcc gtgggcggtg cgcgacctgg
     1021 cgagcggcgc gatcgtgggc tcgacgcgct atcacgacat catcgcggcg gccgaccggg
     1081 tcgagatcgg ctggacctgg tatgcgcagc gggttcagcg cagccacgtg aacacggcgt
     1141 gcaagctgct gctcttcggc tacgctttcg aatcgctcaa atgtaaggtc gtgggcttgc
     1201 gcaccgacaa tttcaatttc cgctcgcagc gcgcgatcga ggcgctcggc gcgaaaaagg
     1261 acggcgtcat ccgccatcac tggccgcggc gcgacggcac gatccgcgac agcgtgatgt
     1321 acagcgtgct cgtgcacgag tggcccgatg tgaagcggca cctggaattc cgcctgaagc
     1381 gaaatgaagc caattagcgt aggccttctc ggcatcggca ccgtgggccg cggcacgtgg
     1441 gatgtcctca accgcaacgc cgacgagatc cagcggcgcg ccggccgcgc gatccgcatt
     1501 gcggtggtgg cggacaaaga gctcgagaag gcaagatcga tcgtgaagga aaaagcgagc
     1561 atcgtggccg atgccttcag cgtggtgcgc ggcagcgagg tcgagatcgt ggtcgagctg
     1621 atcggcggct acaccgtggc gaaggacctc gtgctcgagg cgattgccca tggcaagcat
     1681 gtcgtcaccg cgaacaaggc gctcctcgcc acgcacggca acgagatctt cgccgccgcc
     1741 cagaagaagg gcgtgatggt ggcgttcgag gccgcggtgg cgggcggcgt gccgatcatc
     1801 aaggcgctgc gcgaggggct ctcggcgaac cgcatcgagt ggatcgccgg catcatcaac
     1861 ggcacctcga acttcatcct ctcggaaatg cgcgacaacg gcatcgcgtt cgccgaggcg
     1921 ctgaaggacg cgcagcgccg cggctacgcc gaggccgacc cgaccttcga catcgagggc
     1981 gtggacgcgg cgcacaagct gacgatcctc tcggcgctcg cgttcggcat cccgatgcag
     2041 ctcggcaagt gcttccgcga gggcatccag aagctcaccc gggccgacat cggctacgcc
     2101 gaggagctcg gctaccgcat caagctgctc ggcataacga aaaaagccgc cagcggcata
     2161 gagctgcggg tccatccgac gcttgtgccg gcgcggcggc tgatcgccaa cgtcgagggc
     2221 gtgatgaacg cgatcctggt gaagggcgac gcggtcgggc caacgctcta ctacggcgcc
     2281 ggcgcgggcg cacagccgac cgcgagcgcg gtggtcgccg acctggtcga cgtcacgcgc
     2341 ctcatcaccg ccgacccgga ccagcgcgtg ccgcacctcg cgtttcagcc cgaccagctc
     2401 tcgaacgaga gagtcctttt cattgatgag atcgaaacct cgtactacct gcgcatgcgc
     2461 gtgcaggaca agcccggcgt gctcgccgac atcacgcgca cgctcgccga ctcgagcatc
     2521 tcgatcgagg ccatggtgca gaaggagccg ggcgagggcg agcgtcaggt ggacatcgtg
     2581 cttctcacca accgcgcgct cgaggcggac gtgaaccggg cgatcggcac gatcgagcgg
     2641 ctgcc
//
LOCUS       DASUNT010000398         4405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_582122, whole genome shotgun sequence.
ACCESSION   DASUNT010000398 DASUNT010000000
VERSION     DASUNT010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582122"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..739)
                     /locus_tag="VFX94_08040"
     CDS             complement(53..739)
                     /locus_tag="VFX94_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5768169.1"
                     /translation="MLFALGLVLITPPYALIALATFPLPRMARYRIISGWSRLVVLMA
                     RTIVRIDWRVEGRENLPSRPSVILAKHQSAWETMAFQLIFPPQVLVLKRELLWIPFFG
                     WGLALMSPIAIDRSRGMAALRAIARRGGERLRQGFWVVVFPEGTRVSPGASRAYHPGG
                     AWLACAAGAPVVPVAHNAGLFWPRNAFLKRPGTVTVRIGPPIDAANRDAETVNKLAKT
                     WIEEQQKALT"
     gene            complement(754..1287)
                     /gene="gmhB"
                     /locus_tag="VFX94_08045"
     CDS             complement(754..1287)
                     /gene="gmhB"
                     /locus_tag="VFX94_08045"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEX5768170.1"
                     /translation="MKLVILDRDGTVNEDSDDYVKSPEEWRPIKGSLEAIARLTQADY
                     RVVVATNQSGIARGLFDTRTLFAIHDTMLRALAQHGGRIDAFFFCPHAPEAGCHCRKP
                     QPGMLLEVARRFNVPLDQAYMVGDTQRDLEAAAAAGARPVLVLTGKGAQARDAGKLPA
                     GTRVFPDLAAFAAHLAP"
     gene            complement(1284..3338)
                     /gene="glyS"
                     /locus_tag="VFX94_08050"
     CDS             complement(1284..3338)
                     /gene="glyS"
                     /locus_tag="VFX94_08050"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX5768171.1"
                     /translation="MALTLLVELLTEELPPKSLGALANAFSSALVAALKGDGFLSGNS
                     AATAFATPRRLAVSITDVHERSPDRQLQLTGPSVNAPPQAVAGFAKKNGVSVDELMKL
                     ETPKGEVFAYRRIAAGSHLAANLALRVEEALKRLPVAKLMRWGSGDAQFVRPVHGLVM
                     MHGRQVVPGRVLDLESTNRTQGHRFMGKGTITLADATTYETTLREEGMVIADFAARRD
                     EIERQLKAQAAREKGALGAYEDLLDEVTALVEHPSVYVGSFDAAFLAVPQECLILTMR
                     QNQKYFPLFDGAGKLVPRFLIVSNMKLADPQHIIAGNERVVRPRLEDARFFYEQDRKV
                     RLEARVPELAKVIFHNKLGSQLDRVMRIQMLSGHIARSLSADVAAAERAAWLSKADLL
                     TGMVGEFPELQGVMGRYYALNDGEPRPVADAIQGHYRPRFAGDRLPDDEVACAVALAD
                     KLDALAGMFGIGQQPTGEKDPFGLRRAALGVVRVLVERKLGLSWHALVDSAFNTYASK
                     VKDARAEMHAFVLERFAGYLKDQGFSTLQVDAVLSQNPARLDLVPQQLEAVRAFQELP
                     EAESLAAANKRVANILRQAAAKGESFKPVERKALKEPAELALFTALEMTRGNALPMFE
                     RGDYTGYLKSFAVLKKPVDAFFDSVMVMVDDAGLRQGRLALLNDLRDAMNRVADISKL
                     AQ"
     gene            complement(3339..4217)
                     /gene="glyQ"
                     /locus_tag="VFX94_08055"
     CDS             complement(3339..4217)
                     /gene="glyQ"
                     /locus_tag="VFX94_08055"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEX5768172.1"
                     /translation="MPTFQQLIGQLNAYWDRQGCALLQPYDMEVGAGTFHTATFLRAI
                     GPEPWSAAYVQPSRRPKDGRYGDNPNRLQHYYQYQVVLKPSPAEIQDKYLGSLKDLGI
                     DPAQHDIRFVEDDWESPTLGAWGLGWEVWLDGMEITQFTYFQEVGGLACKPVLGEITY
                     GLERLAMYLQGKDSVFDLIWTPGVAYRDVYHQNEVEQSKYNFELSNADMLLRHFGDFE
                     AEAKRLIAARCVLPAYEMVMKASHTFNLLDARGAISVTERQAYIGRVRTLAKEVAHGY
                     YESRERLGFPMLSKAT"
BASE COUNT          757 a         1435 c         1448 g          765 t
ORIGIN      
        1 cgccggcgcc ggcgcacgaa gcggtactcc acctcgcacc cgcccaggcg gatcaggtga
       61 gcgctttttg ctgttcttcg atccatgtct tcgccagctt gttgaccgtc tcggcgtcgc
      121 ggttggcggc gtcgatcggc ggcccgatgc gcacggtgac cgtgccgggc cgcttcagga
      181 aggcgttgcg tggccagaag agtcccgcgt tgtgcgccac ggggaccacc ggcgcgcctg
      241 ccgcgcacgc gagccaggcg ccgccgggat gataggcgcg cgaagcgccg ggtgagaccc
      301 gagttccttc cggaaagacc accacccaga agccctgccg cagccgctct ccgccgcgcc
      361 gggcgatcgc gcgcagcgcc gccatgccgc gcgagcggtc gatcgcgatc gggctcatca
      421 gggcgagtcc ccagccgaag aacgggatcc agaggagctc gcgcttgagc accagcacct
      481 gcggcggaaa gatgagctga aacgccatgg tttcccatgc cgactggtgc ttggcgagga
      541 tcacggaggg gcgcgagggc agattttcgc gcccctcgac ccgccagtcg atgcgcacga
      601 tcgttctcgc catgagcaca accaggcgcg accagccgga gatgatgcga tagcgcgcca
      661 tgcgtggcag cggaaaggtg gcgagcgcaa tcagcgcgta cggcggggtg atgagaacga
      721 gtcccagcgc gaacaacacc gaacgcacaa atctcacgga gcaaggtgcg cggcgaatgc
      781 cgcgagatcg ggaaacaccc gggttccggc cggcagcttc ccggcgtcgc gcgcctgcgc
      841 gcccttgccg gtgagcacga gcactggccg cgctccggcc gcggcggcgg cctcgaggtc
      901 gcgctgagta tcgcccacca tgtaggcctg gtcgagcgga acattgaagc ggcgcgccac
      961 ctcgagcagc atgcccggct gcggcttgcg gcaatggcag cccgcttccg gcgcgtgcgg
     1021 gcagaagaaa aacgcgtcga tgcgtccgcc gtgctgggcg agcgcacgca gcatcgtgtc
     1081 gtggatcgcg aagagcgtgc gtgtgtcgaa cagcccgcgc gcgattcccg actggttggt
     1141 cgcgacgacg acccggtagt cggcctgtgt gagccgcgcg atcgcttcca ggctgccctt
     1201 gatcgggcgc cactcttcgg gcgacttgac atagtcgtcg ctgtcttcgt tgaccgtacc
     1261 gtcgcggtcc aggatgacga gcttcattgc gctagcttgg agatgtcggc gacccgattc
     1321 atcgcatcgc gcaggtcgtt gagcagcgcg agacggcctt ggcgaagccc ggcatcgtcg
     1381 accatcacca tgacggagtc gaagaaggcg tccaccggct tcttcaggac ggcgaagctc
     1441 ttaagatagc cggtgtagtc gccgcgctcg aacataggca gcgcattgcc tcgggtcatc
     1501 tcgagcgcgg tgaagagcgc gagctcggcc ggctccttca gtgccttgcg ctccaccggc
     1561 ttaaacgatt ctcccttggc tgccgcctgt cgcaggatgt tagcgacgcg cttgttcgct
     1621 gcggcgaggc tttcggcttc gggcagctct tggaaagccc gtaccgcctc aagctgctgc
     1681 ggcacgagat ctaggcgggc cggattctgg ctcagcacgg cgtccacctg aagcgtcgaa
     1741 aatccttggt cctttaggta gccggcgaaa cgctcgagga cgaaagcatg catctccgcg
     1801 cgggcgtcct tgaccttgct cgcatatgtg ttgaacgccg agtcgaccag agcgtgccac
     1861 gataagccga gcttgcgctc gaccaggact cgaaccacgc cgagcgcggc gcggcgcagg
     1921 ccgaaagggt ctttctcgcc agtgggctgc tggccgatgc cgaacatgcc ggcgagcgcg
     1981 tcgagcttgt cggcaagcgc gaccgcgcag gccacttcgt cgtcgggaag ccgatcgccg
     2041 gcgaagcgcg ggcggtagtg cccctggatc gcatcggcga ccgggcgcgg ctcgccgtca
     2101 ttgagcgcgt agtagcggcc catcacgccc tgcagctccg ggaattcgcc aaccatgccg
     2161 gtgagaagat cggccttcga cagccatgcg gcgcgctccg ccgcggcaac gtcggcactc
     2221 aggcttctcg cgatatggcc cgaaagcatc tggatgcgca tgacgcggtc gagctggctg
     2281 ccgagcttgt tgtggaagat tactttggcg agctccggca cccgcgcttc cagcctgacc
     2341 ttgcggtcct gctcgtagaa gaagcgtgcg tcctcgaggc gcggccgcac gacgcgctcg
     2401 ttgccggcga tgatgtgctg cgggtcggcg agcttcatgt tggatacgat gaggaacctg
     2461 ggcacaagct tgccggcgcc atcgaagagc gggaagtact tctggttctg ccgcatcgtg
     2521 aggatcaggc attcctgcgg tacggcgagg aacgcggcgt cgaagctgcc gacatagacg
     2581 ctcggatgct cgacgagcgc ggtcacttcg tcgagcagat cttcgtaggc acctagcgct
     2641 cccttttcgc gtgccgcctg ggccttgagc tggcgctcga tctcatcgcg tcgggcggcg
     2701 aaatccgcga tcaccatgcc ttcctcgcgc agcgtcgtct cgtacgtcgt ggcatccgcg
     2761 agtgtaatcg tgcctttgcc catgaagcgg tggccctggg tgcgattggt ggactccagg
     2821 tccagcacgc gtccgggcac gacctgccgg ccgtgcatca tgacgaggcc gtgcaccggt
     2881 cggacgaact gcgcatcgcc gctcccccag cgcatgagct tcgcgaccgg aagccgcttc
     2941 agcgcctctt cgacccggag cgcaagatta gcggcgagat gagaccctgc cgcaatcctc
     3001 cggtatgcga aaacctctcc tttcggcgtc tcaagtttca tcaattcgtc gacagatacc
     3061 ccgtttttct ttgcgaaccc cgcgaccgct tgcggcggcg cgtttaccga agggcccgtc
     3121 agctgaagct gccgatccgg agatctctca tgcacatccg tgatcgaaac ggcaagccga
     3181 cgcggagtcg cgaacgccgt ggctgcgctg ttgccgctga gaaagccgtc ccctttcagc
     3241 gccgcgacca gcgcgcttga aaatgcattc gccaacgcgc ccagcgactt gggcggcaac
     3301 tcctcggtca gcagctcgac gagcagcgtg agcgccatct atgtcgcctt cgacagcatg
     3361 ggaaagccga ggcgttcgcg cgactcgtaa tacccatggg cgacttcctt cgcgagcgta
     3421 cgcacgcgac cgatataggc ctggcgctcg gtaaccgaga tggcgccgcg cgcatcgagc
     3481 aggttgaagg tgtgggaggc cttcatcacc atctcatagg ccggcaggac gcatcgcgcg
     3541 gcgatcaggc gttttgcctc agcctcgaag tcgccgaaat gccgcaacaa catatcggca
     3601 ttggagagct cgaagttgta ctttgactgc tccacttcgt tctggtggta cacgtcgcga
     3661 taagcgacac ccggcgtcca gatgaggtcg aacacgctgt ccttgccctg caggtacatg
     3721 gcgaggcgct ccaggccgta ggtgatctca ccgagcaccg gcttgcaggc gaggccgccg
     3781 acttcctgga aataagtgaa ctgcgtgatt tccatgccgt cgagccacac ttcccatccg
     3841 aggccccagg cgccaagagt cggcgattcc cagtcgtcct cgacaaagcg gatgtcgtgc
     3901 tgcgccgggt cgattcccaa gtccttcagc gagccgaggt acttgtcctg gatttccgcc
     3961 ggcgagggct tgagcacaac ctggtattgg tagtaatgct gtagacggtt ggggttatcg
     4021 ccatagcggc cgtccttggg tcggcgcgac ggctgcacgt acgccgcgct ccacggctcg
     4081 ggaccgatcg cgcgcaggaa ggtcgcggtg tggaacgtgc ccgcgccgac ctccatgtcg
     4141 tagggctgca gcagtgcgca tccttgccgg tcccagtatg cattgagctg cccgatgagc
     4201 tgctggaatg tcggcacgcg cggattctaa cggctgcgct tcgccctcac ggtgaacaac
     4261 gcgaaaatca gaaggcaggc gacgaccacc ggccagtcgc gcaggcgaac gtacggcgtg
     4321 gctcccgcat atccctgcgc cgggatctcg agccggccct cggtgaactg ctccaggcga
     4381 gcgagcacgc gcccgtcgcg gtcaa
//
LOCUS       DASUNT010000399         3730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_757863, whole genome shotgun sequence.
ACCESSION   DASUNT010000399 DASUNT010000000
VERSION     DASUNT010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757863"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VFX94_08060"
     CDS             complement(<1..483)
                     /locus_tag="VFX94_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768173.1"
                     /translation="MLHHERSGRRRDSMQGVEYIAQLDEAPQERSTIRSTCVAPKRSL
                     SPFSQPQKRRPGRVKDKTTRLAQGIRNQHMAMSVAAGLARAQLVPNPVGVYDARHLSD
                     MLQVMAAAIARVAPIHAMNARTGELRQLSTEELEAATFRHGATLLVLKDGTTISSVTI
                     T"
     gene            complement(682..3105)
                     /locus_tag="VFX94_08065"
     CDS             complement(682..3105)
                     /locus_tag="VFX94_08065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5768174.1"
                     /translation="MLHSSFAWPYVAAIAVPVALLAAAGWLNLRQIEDEAFRRIARAS
                     QALSEHAQRSFRTHEVIIDAVDRHIAGWSWEHILASRELHEVLAQLAKQAPDVASVFV
                     MHPGGGHFNSSRRFPMPAIDGSDRDYYKALREHDVVHVSEPMTGRLSNDRFFSIARRR
                     ALGASGFDGVISVSVNPEYFEKFYETMTETAQDSVVLVRRDGVVLARYPRAEREARIP
                     AANFEKLPAGGTTRVRSAIDGVERLAAFRPVGDYGVYAGYGLSKAAVWATWRKAMIPY
                     AVVTLLAAAALVFAARRRDTHHAETARRGEEERLRRWTDAVPALVSYIDRDLRYRFNN
                     RAYENWFGVRREAMVGRHVAEVLGEAAYEKLKPHFEAALAGRSVTYEALAPYRDGGPR
                     HIQASYIPDVGPDGVRGMFVLVLDITARKQAELALAESDRAKDEFLAMLAHELRNPLA
                     PIVTALEVMKRTAAGSPEALELRARLERQAAHLARLVDDLLDVSRISRGRLEVRRSPI
                     AVAELVAQALETCRPHLEAAGHHVELAMPSEPLYVDADRVRMVQALSNLINNAAKYTR
                     AGGLVSVSAGHAGERVEIRVRDTGIGIAPEMLPRVFDMFARAEHSYESAAGLGIGLTL
                     AKRIAELHDGTLSAHSEGLGRGAQFTLSLPLAPAPSAVAMPAPGPPHQPASGRRVLVI
                     DDNEDAAASLAALLRLSGNDAMVVHDGEEGVRAAISWRPEVILLDIGLPKLNGYEACR
                     RLRAHSATAGARIIALTGWGQEQDRKNSRDAGFDAHLVKPVDYDELDRLLGASPAASQ
                     AHARAAGRR"
     gene            3327..>3730
                     /locus_tag="VFX94_08070"
     CDS             3327..>3730
                     /locus_tag="VFX94_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5768175.1"
                     /translation="MRKLGPSATDIIRADHTRVVAAFHRYKADSSPARKKLIVNLIST
                     ALQAHARMEEEIFYPAMRAAGSTLLDELEPEHEEMRTLIATLSGMQPTDPQYDQTFME
                     LMRAVIHHVADEASRGLMIGAGALLAGLYLLR"
BASE COUNT          566 a         1268 c         1286 g          610 t
ORIGIN      
        1 cgtgatcgtg acgctcgata tcgttgtgcc gtccttcagt accagcaagg tggcgccgtg
       61 cctgaatgtt gcggcctcga gctcctcggt ggaaagctgt ctgagctccc cggtgcgggc
      121 attcatcgcg tggatcggcg cgacgcgcgc gatcgcggcc gccatgactt ggagcatgtc
      181 gctcaggtgg cgtgcgtcgt agacgcccac cggattggga acgagctgtg cacgcgcgag
      241 cccggccgcc accgacatcg ccatatgctg gttgcggatg ccctgggcaa gccgggtggt
      301 tttgtccttc actcgccccg gcctacgctt ttgcggctgc gagaagggtg agagcgatcg
      361 cttcggcgcg acgcatgtgg agcggatggt agagcgttcc tgcggcgcct cgtcaagctg
      421 cgcgatgtac tcgacgccct gcattgaatc gcgccggcgc ccgctgcgct cgtggtgcag
      481 catcgaggca acgatgcgaa ctttgaccgg tcgggaggtg cgactgctcg agctgcgcgc
      541 gcgccgcccg cccgttcgcg accgtgcgcc tggtgagcgg ctggtttgcc attgcacacc
      601 atagcacgcc cccgggcccg ttgtgcggcg caagttgccc ttttactgac tggctgacgc
      661 agcgccggga acgcgctacc gtcagcgcct cccggctgct ctagcgtgcg cctgcgaagc
      721 cgccgggctc gcgcccagca agcgatcgag ctcgtcatag tccaccggct tgacgaggtg
      781 cgcgtcgaag cctgcgtcgc gcgaattttt ccggtcctgc tcctgtcccc agccggtcag
      841 cgcgatgatg cgtgcgcccg ccgtcgcgct gtgcgcgcgc agacgccggc aagcttcgta
      901 gccgttcagc ttcggcagcc cgatgtcgag gaggatcact tcgggacgcc acgagatcgc
      961 tgcgcgcacg ccttcctccc cgtcgtggac caccatggcg tcgttgccgc tgaggcgaag
     1021 cagcgcagcg aggctcgccg cagcatcctc gttgtcgtcg atgaccagca cgcgccggcc
     1081 ggaggcaggc tggtggggcg gccccggtgc cggcatcgcg actgcgctcg gcgccggcgc
     1141 gaggggcagg gaaagcgtga actgcgcgcc gcgcccgagg ccctcgctgt gcgcgctcag
     1201 tgttccgtcg tgcaattcgg cgatgcgctt cgccagcgta agcccgatac cgagccctgc
     1261 cgcgctctca taggagtgct cggcgcgggc gaacatgtca aatacgcgcg gcagcatctc
     1321 cggggcgatg ccgatgccgg tgtcgcgcac acggatctcc acgcgctcgc cggcgtgccc
     1381 ggcgcttacc gagacgaggc cgccggcgcg cgtgtacttg gccgcgttgt tgatcaggtt
     1441 ggaaagcgcc tgcaccatgc gcacgcggtc ggcgtcgacg tagagcggct cggacggcat
     1501 ggcaagctcc acgtggtgtc ctgcggcctc gagatgcgga cggcaggtct cgagcgcctg
     1561 ggcgacaagc tcggcgaccg cgatcgggct gcgcctcacc tcgagccggc cgcgcgagat
     1621 gcggctcaca tcaaggagat cgtccaccag ccgcgcgagg tgcgcggcct ggcgctcgag
     1681 cctggcgcgc agctcgagcg cttccgggct gcccgcggcg gtgcgcttca tcacttcgag
     1741 cgccgtgacg atgggcgcga gcgggttcct caactcgtgc gccagcatgg cgagaaactc
     1801 gtccttggcg cggtcgctct cggcgagcgc gagctcggcc tgcttgcgcg cggtgatgtc
     1861 cagcaccagg acgaacatcc cgcgcacgcc atctggcccg acgtcgggaa tgtagctcgc
     1921 ctggatgtgg cgcggaccgc cgtcgcggta cggagcgagc gcctcgtagg ttacgctgcg
     1981 ccccgcgagc gcggcttcga aatggggctt cagcttctcg taggccgcct ctccgagcac
     2041 ctcggcaacg tggcgcccga ccatggcctc gcgccgcacg ccgaaccagt tctcgtacgc
     2101 cctgttgttg aagcggtagc gcaggtcgcg atcaatgtac gacacgagcg cgggcacggc
     2161 gtcggtccag cggcgcagcc gctcctcctc cccccgccgc gccgtctcgg cgtggtgcgt
     2221 atcgcgccgg cgtgccgcga agacgagcgc cgctgccgcg agcagcgtca ccacggcata
     2281 agggatcatc gctttgcgcc acgtggccca taccgccgcc ttggagagcc cgtatccggc
     2341 gtaaacgccg tagtcgccga ccggccgaaa tgcagcgagg cgctcgacgc cgtcgatcgc
     2401 ggagcgcacg cgcgtggtgc cgccggcggg caacttttcg aagttggctg ccggtatgcg
     2461 cgcttcgcgc tcggcgcgcg gatagcgcgc gagcacgacg ccgtcgcggc gcacgagcac
     2521 caccgagtcc tgcgcggtct cggtcatcgt ttcgtaaaac ttctcgaagt actccggatt
     2581 gaccgagacc gagatcacgc cgtcgaagcc gctggcgccg agggcgcgcc ggcgcgcgat
     2641 gctgaagaag cggtcgttgg agagccgccc ggtcatcggc tcgctgacgt gcacgacatc
     2701 gtgctcgcgc agtgccttgt agtaatcgcg gtcgctgcca tcgatggccg gcatgggaaa
     2761 gcgccggctc gagttgaagt ggccgccgcc agggtgcatc acgaatacgc tcgccacgtc
     2821 gggtgcctgc ttcgcgagct gcgcgagaac ctcgtgcagc tcccgcgagg cgaggatgtg
     2881 ctcccagctc cagccggcga tgtgccggtc cacggcgtcg atgatcacct cgtgcgtgcg
     2941 gaacgagcgc tgcgcgtgct cgctcagcgc ctggctcgcg cgcgcgatgc gccggaatgc
     3001 ctcgtcttcg atctggcgca ggttgagcca tcccgccgcg gcgaggagcg cgaccggcac
     3061 cgcgatcgcg gcgacgtagg gccatgcgaa cgaggaatga agcatgatct tcgcgctgcg
     3121 attttggcgt acccgttgct gaagtgacag tagtcggaaa cgctacgcct cgccggctgg
     3181 tatttcgaca gtggactcgc ggtacatagc ttgctgccac ccgtggtgaa aggagccggc
     3241 atggcagacg atctcaagga agtcgggagg gcggacgacc agcgcatcaa cctcgagcag
     3301 gaccacgagc ggccatgcgc gccttgatga gaaagctcgg gccgagcgcg accgacatca
     3361 ttcgcgccga ccacacccgc gtcgttgccg ccttccaccg ctacaaggca gattcgtcgc
     3421 cggcgcggaa gaagctgatc gtgaacctca tttccacggc gctgcaggcg cacgcacgca
     3481 tggaggagga gattttttat cccgcgatgc gcgcggcggg cagcacgctg ctcgacgagc
     3541 tcgagcccga gcacgaggaa atgcgcacgc tcatcgcaac gctctcgggc atgcagccga
     3601 ccgacccgca gtacgaccag acgttcatgg agctcatgcg cgcggtgatc caccacgtcg
     3661 ccgacgaggc gtccaggggc ctcatgatcg gcgccggcgc actgctcgcc gggctgtatc
     3721 ttctgcgaag
//
LOCUS       DASUNT010000400         4709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_932920, whole genome shotgun sequence.
ACCESSION   DASUNT010000400 DASUNT010000000
VERSION     DASUNT010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932920"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VFX94_08075"
     CDS             <1..398
                     /locus_tag="VFX94_08075"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX5768176.1"
                     /translation="PRVELGERLRGLARAAIDISDGLAGDLGHIAARSQVAATLDYER
                     IPRAPALRALGAAELERDCVLSGGDDYELLFTAPREQRPEIESLAAELGLAITRIGVI
                     EAGSGLRILDAEGMPIAHRGGFDHFGDRR"
     gene            395..880
                     /locus_tag="VFX94_08080"
     CDS             395..880
                     /locus_tag="VFX94_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEX5768177.1"
                     /translation="MIALRPRASFAFSHPAHAIAFGFGAGLAPVAPGTFGTALGWAIG
                     WALAALHPALFGSLIVVSFLVGLWACEVTGRDMGVADHGAIVWDEVVAFLLVLSVVPR
                     ELGWQAAAFAVFRFFDIAKPPPIRSLERRFGGGFGVMFDDIVAAAYTLLFLAAVKRAF
                     F"
     gene            880..1371
                     /locus_tag="VFX94_08085"
     CDS             880..1371
                     /locus_tag="VFX94_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX5768178.1"
                     /translation="MDELARRVGERLAASGQRLATAESCTGGWVAQAITAIAGSSDWF
                     DRGFITYSNEAKQEMLGVRADTLARHGAVSEDTAREMVQGALVRSKSTLALAVTGIAG
                     PGGGSPQKPVGMVCFAWASRQSIRAETKHFSGDRESVRRLSVIHALEGVLKTLTAAER
                     GHT"
     gene            1380..2726
                     /gene="amrB"
                     /locus_tag="VFX94_08090"
     CDS             1380..2726
                     /gene="amrB"
                     /locus_tag="VFX94_08090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX5768179.1"
                     /translation="MTSVRPAAVAGMFYPGDARALAAEVDDLLGGVSQPQPRLGHPKA
                     LIVPHAGYIYSGGIAARAYDELAAARGSVKRVVLLGPTHRVAVRGLAVPSAGGFATPL
                     GTVRVDTEALDSVRDLPQVVVNDACHAMEHSLEVQLPFLQSMLGEFALAPFAVGMASV
                     AEVAEVIARLWGGAETVVVVSTDMSHYHSYEQARAIDRATILRIASFATDIHHEEACG
                     ATLLNGLLSFSRQKNLSLKLLAACNSGDTAGGKGRVVGYSAFGIYEGGEVSLDEAGRT
                     LLSLARDTIEDSLISRTTSKSYSSWLSQTGATFVTLTKDGNLRGCIGSLQPARALHED
                     VAQNALAAAFRDPRFPAMTAAEWPQCRVEVSLLSTPKPVRFADEADLLEQIEAGKDGL
                     ILEADGKRATFLPQVWEDLAEKRVFLAHLLKKAGLPADTRLTRCRISRYRVAKWKE"
     gene            2714..3802
                     /gene="amrS"
                     /locus_tag="VFX94_08095"
     CDS             2714..3802
                     /gene="amrS"
                     /locus_tag="VFX94_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX5768180.1"
                     /translation="MERVDTSYPGRWWHTLPDGRVQCDLCPRDCKLHEGQRGLCFVRK
                     MEQGRMVLTTYGRSSGFCIDPVEKKPLNHFYPGTSILSFGTAGCNLACKFCQNWDISK
                     SREMDRLQDQASPQAIAAAALRLGCKSVAFTYNDPVIFAEYAMDIADACHAKGVKTVA
                     VTAGYIHTEPRREFYAKMDAANVDLKAFTDDFYWKICGGRLQPVLDTLVYLVKETHVW
                     TEITTLLIPGKNDSDEEIKAECKWIRANLGADVPLHFTAFHPDWKMTDIPRTPAVKLT
                     RAREIAQLAGLNYVYTGNVHDESGGSTYCPSCDKPVVRRDWYDIRGYALDASGACKHC
                     GTAIPGRFQEFGKAFGPRRIPVRLEQTA"
     gene            3860..4435
                     /locus_tag="VFX94_08100"
     CDS             3860..4435
                     /locus_tag="VFX94_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEX5768181.1"
                     /translation="MSRMKPFSEASERNRRPILAVLKRVFANSRFVLEIGSGTGQHAA
                     YFAPELPHLVWQASDVAQNLPGIREWVSAPRPIELDVEKEWPELEADAVFSANTCHIM
                     SWPQVERMFERIGRIPNLNIFCLYGPFNYGGRHTSESNARFDAMLRARDAQSGLRNFE
                     DMAALGKRAGLLLEEDNAMPANNRLLVFRRS"
     gene            4445..4588
                     /locus_tag="VFX94_08105"
     CDS             4445..4588
                     /locus_tag="VFX94_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768182.1"
                     /translation="MPPGQRATIVGANLLVPAGVLFFGWNAAAAAFPMDTELHENWLA
                     QIA"
BASE COUNT          767 a         1592 c         1555 g          795 t
ORIGIN      
        1 agccgcgcgt cgagctcggc gagcgtctgc gcgggcttgc gcgcgcggcg atcgacatct
       61 ccgacggcct tgcgggcgac cttggccaca tcgccgctcg gtcgcaagtc gctgcaacgc
      121 tcgactacga gcggattccc cgagcgccgg ctcttcgggc actgggcgcc gcggagctcg
      181 agcgcgactg tgtgctttcc ggcggcgacg actacgagct cctcttcacc gccccgcgcg
      241 agcagcgccc cgaaatcgag tcactcgccg cggagctcgg gctcgccatt acgcggatcg
      301 gcgtcatcga ggcaggctcg ggcttgcgca tcctcgatgc cgaaggcatg ccgattgccc
      361 accgcggcgg cttcgaccac ttcggggacc ggaggtgatc gccctgcgcc cgcgtgcttc
      421 gttcgccttc tcgcatcctg cgcacgcgat cgccttcggc ttcggcgccg ggcttgcgcc
      481 cgtcgcgccc gggactttcg gcaccgcgct cggctgggcg atcggctggg cgctggcagc
      541 gctgcatccg gcgctgttcg gctccctgat cgtggtgtct ttcctcgtcg gcctttgggc
      601 gtgcgaggtg accggccgcg atatgggcgt cgccgaccac ggcgcgatcg tatgggacga
      661 ggtggtcgcg tttctcctcg tgctttccgt cgtcccgcgc gagctcggct ggcaggcagc
      721 cgcgtttgcc gtcttccgct ttttcgatat cgcgaagccg ccgcccatcc ggagcctcga
      781 gcggcgattc ggcggcggct ttggtgtcat gttcgacgac atcgtcgctg ccgcgtacac
      841 gctgcttttt cttgcggcgg tgaaaagagc gttcttctga tggatgagct ggcgcggcgc
      901 gtcggcgagc ggctcgccgc ctccgggcaa aggctggcga cggcggagtc gtgtaccggc
      961 ggctgggtgg cgcaggctat cacggcgatc gccgggtcct ccgactggtt cgaccgcggc
     1021 ttcatcacct actccaacga ggcgaagcag gagatgctcg gcgtgcgcgc cgacacgctc
     1081 gcgcggcacg gcgcggtgag cgaagacacg gcacgcgaga tggtgcaggg cgcgcttgtg
     1141 cgcagcaaat ccacgttggc gctcgcggtc accggcatcg ccgggcccgg gggtgggagc
     1201 ccgcaaaagc cggtcggcat ggtgtgcttt gcctgggcga gccgccagag cattcgcgcc
     1261 gagacgaagc atttctccgg cgatcgggaa agcgtgcgcc ggctttcggt catccacgcg
     1321 ctcgagggcg tactgaagac cttgacggcc gctgagcgcg gccatacata gataagcgca
     1381 tgacttcggt tcgcccggca gccgtagcgg ggatgttcta cccgggggac gcgcgcgcgc
     1441 ttgcggctga agtggacgat ctgctcggcg gcgtaagcca gccgcagccg cgcctgggcc
     1501 atcccaaggc gctcatcgtg ccgcacgccg gctacatcta ttccggcggc atcgcggcgc
     1561 gcgcctacga cgagctcgct gccgcgcgcg ggagcgtaaa gcgcgtggtc ctgctcgggc
     1621 ccacgcaccg ggtggcggtg agagggctcg cggtgccgag cgccggcggc ttcgccacgc
     1681 cgctcggcac cgtgcgtgtc gacaccgagg cgctcgactc ggtgcgcgac ctgccgcagg
     1741 tcgtcgtaaa cgacgcgtgc catgccatgg agcactccct cgaggtccag ctgcctttcc
     1801 tgcagagcat gctcggcgag ttcgcgcttg cgccgttcgc cgtcggcatg gcaagcgtcg
     1861 ccgaggtcgc ggaggtgatc gctcggctct ggggcggcgc cgaaacggtg gtggtagtct
     1921 cgaccgacat gtcgcactac cactcctacg agcaggcacg cgccatcgac agggccacga
     1981 tcttgcgcat cgcttccttt gcgaccgata ttcatcacga ggaagcctgc ggcgcgacgc
     2041 tgctcaacgg gcttttgtcg ttttcaagac aaaagaatct gtccctgaag ctgctcgccg
     2101 cgtgcaactc cggcgatacc gcgggcggca agggcagggt ggtcggctat tcggccttcg
     2161 gcatctacga aggcggcgag gtttctctcg acgaagccgg gcgcacgctg ctttcccttg
     2221 cccgcgacac catcgaagat tcccttatca gcaggaccac ttcgaaaagc tattcttcct
     2281 ggctcagcca gaccggcgcc acgttcgtca cgctcaccaa ggacggaaac ctgcgcggct
     2341 gcatcggcag cctgcagccg gcgcgcgcgc ttcatgagga cgtggcgcag aacgcgcttg
     2401 ccgcggcatt ccgcgacccg cgctttccgg ccatgacggc cgccgaatgg ccgcaatgcc
     2461 gcgtcgaggt gtcgctgctt tccacgccga agcctgtgcg cttcgccgac gaggctgatc
     2521 tgctcgagca gatagaggcg ggcaaagacg ggttgatcct cgaggccgac ggaaagcgcg
     2581 ccacgttcct gccgcaggtg tgggaagacc tcgccgagaa gcgcgtcttc cttgcccacc
     2641 tgttgaaaaa agccggcttg ccggcggaca cgcgcctgac gcgctgcagg atatcgcgct
     2701 accgggtcgc taaatggaaa gagtagacac ttcctatccg ggccgctggt ggcacacgct
     2761 gcccgacggc cgcgtgcagt gcgacctgtg tcctcgggac tgcaagctgc acgagggtca
     2821 gcgcggcctt tgcttcgtgc gcaagatgga gcagggccgc atggtgctca ccacctacgg
     2881 ccgctcctcg ggtttctgca tcgaccccgt ggagaagaag ccgcttaacc atttctaccc
     2941 cggcacttcg atcctctcgt tcggcaccgc ggggtgcaac ctggcgtgca agttctgcca
     3001 gaactgggac atctccaagt cgcgcgagat ggatcggctg caggaccagg cgagcccgca
     3061 ggccatcgcc gcggcggcgc tccggctggg ctgcaagtca gtcgccttca cctacaacga
     3121 cccggtgatt ttcgccgagt acgcgatgga catcgccgat gcctgccacg ccaagggcgt
     3181 gaagaccgtg gccgttaccg caggctacat ccacaccgag ccgcggcgcg agttctacgc
     3241 gaagatggac gcggcgaacg tcgacctgaa agccttcacc gacgacttct actggaagat
     3301 ctgcggcggc cgcctgcagc cggtgctcga cacgctcgtc tacctggtga aggaaaccca
     3361 cgtctggacc gagatcacca cgctgctcat cccgggcaag aatgattccg acgaggagat
     3421 taaggccgag tgcaagtgga tccgcgcgaa cctgggggcc gacgtgccgc tgcacttcac
     3481 cgccttccat cccgactgga agatgaccga catcccgcgc acgccggcgg tcaagctcac
     3541 gcgcgcgcgc gagatcgccc agctcgccgg cctgaattac gtctataccg gcaacgtgca
     3601 cgacgaatcc ggcggctcca cgtactgtcc ttcgtgcgac aagccggtgg tccgccgcga
     3661 ctggtacgac atccgcggct acgcgctcga cgcctcgggc gcctgcaagc actgcggcac
     3721 ggcgattccc ggccgcttcc aggaattcgg caaggccttc gggccccgcc gaattcccgt
     3781 caggctcgaa cagaccgcct aagccagcag agaattgctc gcgccgcagc gacggcacgt
     3841 cggtgatcag cccggcagaa tgtcgcgcat gaagcccttc tcggaagcga gcgagcgcaa
     3901 ccgcaggccg atccttgcgg tcctgaaacg agtctttgca aacagccgct tcgtgctcga
     3961 gatcggatct ggcacgggcc agcacgccgc gtacttcgcg ccggagctgc cgcacctcgt
     4021 atggcaggcg agcgacgtag cgcagaacct gcccgggatc cgcgaatggg tgtccgcgcc
     4081 gcgaccgatc gagctcgacg tagagaagga gtggcccgag ctcgaagcgg acgccgtgtt
     4141 ctcggccaac acctgccaca tcatgtcctg gccgcaggtc gagcgaatgt tcgaaaggat
     4201 cggaaggatc ccgaatctga acatcttctg tctctacggc cccttcaact atggcggcag
     4261 gcacacctcc gagagcaacg cccgcttcga cgccatgctg cgcgcccgcg acgcgcagag
     4321 tggacttcgg aacttcgagg acatggcggc gctgggaaag cgcgccggcc ttttgctcga
     4381 ggaggacaat gcgatgccgg cgaacaaccg gctgctagta ttccggcgca gctaagggag
     4441 cctcttgccg ccaggccagc gcgcgacgat tgtcggagcc aaccttctcg ttcccgccgg
     4501 agtgctgttc ttcggctgga acgcggccgc cgcggcgttc cctatggata cggagctgca
     4561 cgagaactgg ctcgcgcaga tcgcttaggc gagcacaggc tgctcgagcg gcggcgagcg
     4621 cggcaaaatg ccgcgcatga agcccttctc tgaagcgagc gagcgcaacc gcgccccgat
     4681 cctcgcgatc ctgaaaagag tgtttgcca
//
LOCUS       DASUNT010000401         5966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_145414, whole genome shotgun sequence.
ACCESSION   DASUNT010000401 DASUNT010000000
VERSION     DASUNT010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145414"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..1759
                     /locus_tag="VFX94_08110"
     CDS             233..1759
                     /locus_tag="VFX94_08110"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX5768183.1"
                     /translation="MQLEREIKFSLPAAAARRVGRQVRRAGPWRRRMVFNAYYDTANG
                     RLRRAGVALRLRRDGKRQLQTLKVEADGAGLTTRAEWEMPAPGGRLHLEAFPREEILA
                     ATGLDMTRLVRQLRPCFETRFARRSAPVLVDGATRAEISIDRGYVAAGERREPISEVE
                     IELKAGDAASLLRYAGELAKPLGLTLEFESKAERGYRLLAGEAFPPPRKWRRPKLGEL
                     ATPSEAFRSIFGAALTQAGANARGVAHGRDPEYLHQMRVGLRRLRSALLAFRDLVPKK
                     AATPLVERLRGLMPDLGAARDWDVFSESLVRLGIQEPDRAPLLAPLLARARARRAAAR
                     RRARIAATSARLQSFLLRAMRWVNDEPWKRRAEDTEGSLGAFAAAALERLHGRALRDA
                     KDIDWADAERRHRLRIRMKRLRYACDFFAPSFAGAAAMPYIKRLAALQDILGDLNDIA
                     VARTLLAEIAPRGSARDVASAAAHVRHALAVRERMLVISLQEAWAAFEKRRPFWRARG
                     "
     gene            complement(1692..2540)
                     /gene="prmB"
                     /locus_tag="VFX94_08115"
     CDS             complement(1692..2540)
                     /gene="prmB"
                     /locus_tag="VFX94_08115"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: protein motif:HMM:TIGR03533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX5768184.1"
                     /translation="MTLEQLIRQTAKRLQAARLHYGHGTDNAHDEAAFLVLRGLGLPF
                     DADLHRNADARRIAPLVDRRIRQRIPAAYLLHEAWLDGVAFYVDRRVIIPRSHIAFLL
                     KDLRIAPRRILDLCTGSGCLAILAARAFPKARVDASDVSSAALEVAARNVGKHACRRR
                     IRLIRSDLFASIRGPYDLILSNPPYVSTPAMRRLPREYRYEPGIALAAGRLGLDYVAR
                     IIEESPPFLHPGGLLVCEVGDNQASLRRAYPRTPFIWPLPQVFTLAHARTAGVSRKRP
                     TPPAAR"
     gene            complement(2537..3664)
                     /gene="dapE"
                     /locus_tag="VFX94_08120"
     CDS             complement(2537..3664)
                     /gene="dapE"
                     /locus_tag="VFX94_08120"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEX5768185.1"
                     /translation="MPEALELAKELIARRSVTPEDGGCQDLIAKRLQRAGFRCEPMKF
                     GEVTNLWARRGTEHPVVCFAGHTDVVPTGPLGEWHSDPFVPTIRDGKLFGRGAADMKS
                     SIAAFVAATEMFVQERPRHSGSIAFLLTSDEEGPAVDGTVRVVEALKRRNELIDYCIV
                     GEPTSVDALGDMLKNGRRGSLSANLTVRGVQGHVAYPHLARNPVHELAPALAELARVQ
                     WDKGNEDFPPTTWQVSNLRAGTGAANVIPGTVEVDFNFRFSTESTDAALKARLESILK
                     KHGLDYSVRWTLGARPFLSKRGKLAQTVMAAGKKHTGRSAELSTTGGTSDARFIIDIC
                     PEIIELGPVNATIHKLNEHIAVEELDLLPRIYLDVLRALLP"
     gene            complement(3657..4844)
                     /locus_tag="VFX94_08125"
     CDS             complement(3657..4844)
                     /locus_tag="VFX94_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX5768186.1"
                     /translation="MSAMKRLFQIMADKKASDIFISVGAPINIKINGNAMPVNQTTMT
                     TETVQQLLYEVLNERQIREFEDEMELNTAYNLAGVGTFRISAFRQKGSPSVVVRYIPA
                     TIPPIDTLGLPEVLKEVIMGKRGLILMVGATGSGKSTSLAAMLDYRNERNTGHILTLE
                     DPVEFIFQNKKSIVNQREVGTDTKEFEIGLRNALRQAPDCILIGEIRDKPTMAMALAY
                     AQSGHLCLATLHANNSYHAMNRIISFYALENRPALLLDLAAALSAVISLRLVRTKTGM
                     RVPAVEVLLNTRHIAELIEKGEIGEIREALEKSMSPGSQTFEQALFKLFMDGKITQDE
                     AMANADSATNMLWLINQATAGEITGQSVPQAAAKPAEPKKEGLLSTGQGASFGNFKID
                     VNA"
     gene            complement(4877..5668)
                     /gene="dapD"
                     /locus_tag="VFX94_08130"
     CDS             complement(4877..5668)
                     /gene="dapD"
                     /locus_tag="VFX94_08130"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX5768187.1"
                     /translation="MSAREVVEAAWETRASLKPTAQLRAAVEEAIAGMDSGRLRVAEK
                     VGGEWVTHQWLKKAVLLSFRLQDNRVIDGRYYDKVDSKFDNFDFASAGFRVVPPAAAR
                     RGAYIAKNVVLMPSFVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEP
                     VQANPVIIEDNCFIGARSEVVEGVIVEENSVLSMGVYLGQSTRILERASGRILYGRVP
                     AGSVVVSGTLPSPDASHSLYCAVIVKTVDAKTRAKTSLNELLRGD"
     gene            complement(5665..>5966)
                     /locus_tag="VFX94_08135"
     CDS             complement(5665..>5966)
                     /locus_tag="VFX94_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEX5768188.1"
                     /translation="HVRENRRLYAEKFAAVLPLVEAKRPDGGFYLWLPTPIDDTEFVR
                     RLHAEYNVLVLPGSYLGRAVNGRNPGARHVRIALVAPLEECVQAMQSVSQLISRL"
BASE COUNT          933 a         1972 c         2027 g         1034 t
ORIGIN      
        1 tcacgatgtt gcgcatgatg acgtcgggcg tgccgccagg ccccgagccg accgtgatct
       61 ccaccggccc tttcggcgcc ttcatctgcg cgatcgcgcc cggggcgagc gcgatgccca
      121 acgcgactgc aacggctttc agcaccatga tcctcctcct gttgtggact tcattgctcg
      181 gggcgagcgt agtcaagcat tccggtaaga tcaagcactt gtctatcgcg acatgcaact
      241 cgagcgtgag atcaagttca gcctgcccgc ggcggcggcg cgccgcgtgg ggcggcaggt
      301 gcgccgcgcg ggtccgtggc gccgccgcat ggttttcaac gcctactacg acaccgcgaa
      361 cgggcggctg cggcgcgcgg gtgtcgcgct gcggctgcgc cgcgacggca agcgccagct
      421 ccagacgctg aaagtcgagg ccgacggcgc cgggctcacc acgcgcgcgg aatgggagat
      481 gccggcgccg ggcgggcggc tgcacctcga ggcgtttcca cgggaggaaa tcctcgccgc
      541 cacgggcctc gacatgacgc gcctcgtacg gcaactgcgc ccttgcttcg agacgcgctt
      601 tgcgcgccgg tcggcgccgg tgctcgtcga cggggctacg cgcgcggaga tttccatcga
      661 tcgcggctac gtcgcggccg gcgagcgccg cgagccgatc agcgaagtgg agatcgagct
      721 caaagccggc gacgcggcgt cgctgctgcg ctatgcgggc gagcttgcca agccgctcgg
      781 gctcacgctc gagttcgaaa gcaaggcgga gcgaggctat cgcctgctgg ccggcgaggc
      841 ttttccgccg ccgcgcaagt ggcgccgtcc gaagctgggc gaactcgcca ccccgagcga
      901 agcgttccgc tcgatcttcg gcgccgcgct cacgcaggcg ggcgccaatg cgcgcggcgt
      961 ggcccatggc cgcgaccccg agtaccttca ccagatgcga gtggggctgc ggcggctgcg
     1021 ctccgcgctg ctcgcgttcc gcgatctcgt gccgaagaaa gctgccacgc cgctggtcga
     1081 gcggctgcgc ggcctgatgc ccgatctcgg cgccgcgcgc gactgggacg tcttcagcga
     1141 aagcctggtg cgcctcggaa tccaggagcc ggaccgcgcg ccgctccttg cgccgttgct
     1201 tgcgcgcgcc cgcgcaaggc gcgcggcggc gcgtcggcgc gcgcgcatcg cggccacgtc
     1261 tgccaggctg cagtcattcc tgctgcgcgc gatgcgctgg gtgaacgacg agccctggaa
     1321 acgcagggcg gaggacaccg agggctcgct cggcgccttc gccgcggcgg cgctcgagcg
     1381 cctgcacggc agggcgttgc gcgatgccaa ggacatcgac tgggccgatg ccgagcggcg
     1441 ccaccgactg cgcatccgca tgaagcgcct gcgctatgcg tgcgatttct tcgccccctc
     1501 ctttgccggc gccgcggcaa tgccctacat caagcgcctc gctgcgctgc aggacatcct
     1561 gggagatctg aacgacatcg cggtggcacg caccctgctt gccgaaatcg ctccgcgcgg
     1621 cagcgcgcgc gacgtggcga gcgctgcggc tcatgtccga cacgcgctcg ccgtgcgcga
     1681 gcgcatgctc gtcatctcgc tgcaggaggc gtgggccgct ttcgagaaac gccggccgtt
     1741 ctggcgtgcg cgagggtgaa cacctggggc agcggccaga tgaacggcgt gcgcggatag
     1801 gcgcgccgca gcgacgcctg gttgtcgccg acctcgcaga cgaggagccc gcccggatgc
     1861 aggaacgggg gcgactcctc gatgatccgc gccacgtaat cgaggccgag cctgcctgcc
     1921 gcgagcgcga tgccgggctc gtagcgatac tctcgcggca gccggcgcat cgccggcgtg
     1981 ctgacatacg gcgggttgga gagaatgagg tcgtatgggc cgcggatgct ggcgaagaga
     2041 tccgagcgga taagacgaat tcgccttcga catgcatgtt tgccgacatt acgagctgcc
     2101 acttccagcg ccgcggagct gacatccgaa gcgtccaccc gggctttggg aaaagcgcgc
     2161 gcggcaagga tcgccaggca acccgacccg gtgcagaggt cgaggatgcg ccgcggcgcg
     2221 atgcgcaggt ccttcagcag gaacgcaatg tgggagcgcg gaatgatgac ccggcgatcg
     2281 acgtagaacg cgacgccgtc gagccacgct tcgtggagaa ggtacgcggc ggggattcgc
     2341 tgcctgattc ttcggtccac caggggcgcg atacggcgcg catcggcgtt gcgatgcaga
     2401 tctgcatcga acggcagacc gaggccgcgc aagacgagaa aagcggcttc gtcgtgcgcg
     2461 ttatcggtgc cgtgcccgta atgcaggcgg gccgcctgca gtcgcttcgc ggtttgtcgt
     2521 atcagctgct ctaacgtcaa ggcagcagag cgcgcagcac atcgagatag atgcgcggga
     2581 gaaggtcgag ctcttccacc gcgatgtgct cgttcagctt gtgaatcgtc gcgttcaccg
     2641 gcccgagctc gatgatctcg gggcagatgt cgatgatgaa gcgcgcatcc gaggttcccc
     2701 cggtggtgga aagctcggcg gagcgcccgg tgtgcttctt gccggcggcc atcaccgtct
     2761 gcgcgagctt gccgcgcttg ctgaggaagg gccgggcgcc aagagtccag cgaacgctgt
     2821 aatcgaggcc gtgcttcttg aggattgatt cgagccttgc cttcagggcg gcgtcggtgc
     2881 tttccgtgga gaagcgaaag ttgaagtcca cctcgaccgt gccgggaatc acgttcgccg
     2941 cgccggtgcc ggcgcgcagg ttcgacacct gccacgtcgt gggcggaaag tcctcgttgc
     3001 ccttgtccca ctgcacccgg gccagctcgg cgagcgcggg cgcgagctcg tgcaccgggt
     3061 tgcgcgcgag gtgagggtag gctacgtggc cttgaactcc cctgacggtc agatttgccg
     3121 aaagcgatcc gcgccggccg ttcttcagca tgtcgccgag cgcgtcgacc gaagtcggct
     3181 cgccgacgat gcagtaatca atcagctcgt tgcgtcgctt gagggcctcg acgacgcgca
     3241 cggtgccgtc caccgcaggc ccttcctcgt cggaggttag gagaaacgcg atcgagcccg
     3301 agtgccgcgg cctctcctgc acgaacattt cggtcgccgc cacgaaagcc gcgatggagg
     3361 atttcatgtc ggcggcgccg cggccgaaaa gcttgccgtc gcggatggtc gggacgaaag
     3421 gatcggagtg ccactccccg agcgggccgg tggggacgac gtcggtatgg cccgcgaagc
     3481 acactaccgg gtgctcggtc ccgcggcgcg cccagaggtt cgtgacctcg ccgaatttca
     3541 tcggctcgca cctgaacccg gcgcgctgca gccgctttgc gatgaggtcc tggcagccgc
     3601 cgtcttcggg ggtgaccgat ctgcgggcaa tgagctcctt ggcgagctct agcgcctcag
     3661 gcattcacgt cgatcttgaa attcccgaac gacgctccct ggccggtgga aagcaggcct
     3721 tccttcttgg gctcggccgg cttggcagcg gcctgcggca ccgactggcc ggtgatctcg
     3781 cctgcggttg cctggttgat gagccagagc atgttggtgg cggagtcggc gttcgccatc
     3841 gcctcgtcct gcgtgatctt gccgtccatg aagagcttga agagcgcctg ctcgaacgtc
     3901 tgcgagcccg gcgacatgct tttttccagc gcctcgcgga tctcgccgat ctcgcccttc
     3961 tcgatcagct cggcgatgtg acgcgtgttg agcagcactt cgaccgccgg cacgcgcatg
     4021 ccggtcttcg tgcgcaccag gcgcagcgag atcacggccg aaagcgccgc tgccaggtcg
     4081 agcagcagcg ccggccggtt ctccagcgcg tagaagctga tgatccggtt catcgcgtgg
     4141 tagctgttgt tcgcgtgcag cgtggcgagg cacaggtgcc ccgactgcgc gtacgcgagc
     4201 gccatcgcca tggtgggctt gtcgcggatc tcgccgatca ggatgcagtc gggggcctgc
     4261 cgcagcgcgt tcctgagccc gatctcgaac tccttggtgt cggtgccgac ctcgcgctgg
     4321 ttcacgatcg acttcttgtt ctggaagatg aactcgacgg ggtcttcgag cgtgaggatg
     4381 tggcccgtgt tgcgctcgtt gcggtagtcg agcatggcgg cgagcgaggt cgacttgccc
     4441 gacccggtcg cgccgaccat cagaatcagg ccgcgtttgc ccatgatgac ttccttcagg
     4501 acctcgggca acccgagcgt gtcgatcggc gggatggtgg cgggaatgta gcgcaccacc
     4561 accgacggcg aacccttctg ccggaaggcg ctgatgcgaa acgtccccac gccggccagg
     4621 ttataggcgg tgttgagttc catctcgtct tcgaactccc ggatctggcg ctcgttcagc
     4681 acctcgtaga ggagctgctg caccgtctcc gtggtcatgg tcgtctgatt caccggcatg
     4741 gcgttgccgt tgatcttgat attgatcggt gcccccaccg agatgaagat gtccgaggcc
     4801 ttcttgtcgg ccatgatctg gaacagccgc ttcatcgcag acatcgtgca gccctcccga
     4861 gctggtgccc gctagtttaa tcccccctga gaagctcgtt caggctggtc ttcgcgcggg
     4921 tcttggcatc aaccgttttt acaatcaccg cacagtacag gctgtggctt gcgtcggggg
     4981 agggcaaagt gcccgaaacc accaccgacc cagccggcac gcgcccatac aggatacgtc
     5041 cgctcgcgcg ctcgaggatg cgcgtgctct gccccagata gacgcccatc gaaaggacgc
     5101 tgttctcctc gacgatgacc ccttctacga cctccgagcg cgcgccgatg aagcagttgt
     5161 cctcgatgat gaccgggttt gcctgtaccg gctcgagcac gccgccgatg ccgacgcctc
     5221 ccgagaggtg cacgttcttc ccgatctgcg cgcacgagcc gacggtcgcc cacgtgtcca
     5281 ccatggtgcc ttcgtcgacg taggcgccga tgttcacgaa cgacggcatg agcaccacgt
     5341 tcttcgcgat gtaggcgccg cggcgcgcgg ccgcgggcgg cacgacgcgg aagcctgccg
     5401 aggcgaagtc gaagttatcg aacttcgagt cgaccttgtc gtaatacctg ccatcgatca
     5461 cccggttgtc ctgcaggcga aaggaaagca gcaccgcctt cttgagccat tggtgcgtga
     5521 cccattcgcc gcccaccttc tccgcgacgc gcagcctccc gctgtccatc ccggcgatcg
     5581 cctcttccac ggcagcgcgc agctgcgccg tgggcttgag cgaggcgcgc gtctcccatg
     5641 cggcttcgac gacttcgcgg gcgctcatag gcggctgatc agttgagaaa cgctttgcat
     5701 ggcttgcacg cactcctcga gaggggcaac cagggcgatg cgcacgtggc gcgcaccggg
     5761 attcctgccg ttcaccgcgc ggccgaggta gctgcccggc agcacgagca cattgtattc
     5821 ggcgtgcagg cgccgcacga attcggtatc gtcgatcggg gtgggcagcc agaggtagaa
     5881 gccgccgtcg ggccgtttcg cctcgacgag cggcagcacc gcggcgaact tctcggcgta
     5941 cagccggcgg ttttcgcgca cgtgct
//
LOCUS       DASUNT010000402         2705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1109111, whole genome shotgun sequence.
ACCESSION   DASUNT010000402 DASUNT010000000
VERSION     DASUNT010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109111"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VFX94_08140"
     CDS             <1..727
                     /locus_tag="VFX94_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899926.1"
                     /note="subunit D of antiporter complex involved in
                     resistance to high concentrations of Na+, K+, Li+ and/or
                     alkali; contains an oxidoreductase domain; catalyzes the
                     transfer of electrons from NADH to ubiquinone; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5768189.1"
                     /translation="LLLYGGMATIAYGISGMLASQTLGRLAGFNLLVSSGTLLAAIGA
                     GHAGATGAALYYLVVSTLGSAAFFLLIELVERARTPGADVLAVTAAAFGIGGEEPEPE
                     HETGVAIPASMALLGLSFVCCALLAAGLPPLPAFVAKFALLAALLGPDPVPGAAWALL
                     VLLTLSGLAAIIAMSRSGVRIFWGGTATPPRVRLIEMAPVAVLLGLCFALTVAAGPAM
                     EYLSRTAHALHAPGGYIESVLTP"
     gene            724..1209
                     /locus_tag="VFX94_08145"
     CDS             724..1209
                     /locus_tag="VFX94_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEX5768190.1"
                     /translation="MTGWLPYPLLSVALFAAWLLLNQSFAAGHVVLGIALGLAGGRIL
                     AGLQPPQRRPRRAAVALELFFLVLADIVRSNIAVARIVLNPGTKRTAGFLSLQLELRH
                     PAGLAVVACIITATPGTSWARYDAARNIVTIHVLDLLDEEEWVHIFKQRYERRLREIF
                     E"
     gene            1206..1487
                     /locus_tag="VFX94_08150"
     CDS             1206..1487
                     /locus_tag="VFX94_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HEX5768191.1"
                     /translation="MNAGLSGAILILAQVFIALAMACALYRLLRGPRAQDRVLALDTL
                     YVNAMLLVLTFGIREATTLYFEAALVIGVMGFVSTAALAKFLLRGEVIE"
     gene            1484..1783
                     /gene="mnhG"
                     /locus_tag="VFX94_08155"
     CDS             1484..1783
                     /gene="mnhG"
                     /locus_tag="VFX94_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HEX5768192.1"
                     /translation="MSTAAALLVLAGASLALIGSIGLLRMKTFYERVHAPTMGTTLGT
                     GLVLLGSMIFFTFHESRPVLHEILIGVFMTLNTPVTYMILVRAALHRDPIDIPRS"
     gene            1811..1990
                     /locus_tag="VFX94_08160"
                     /pseudo
     CDS             1811..1990
                     /locus_tag="VFX94_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520375.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
     gene            2000..2320
                     /gene="sugE"
                     /locus_tag="VFX94_08165"
     CDS             2000..2320
                     /gene="sugE"
                     /locus_tag="VFX94_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEX5768193.1"
                     /translation="MAWTILVIAGLFEVGWAIGLKYTEGFTRLWPTVWTVLAMIVSLW
                     LLGVAMKSLPVGTAYSVWVGVGAVGTVILGIVLLGEPANAARLISIAFIVAGIVGLKL
                     ATPG"
     gene            2459..>2705
                     /locus_tag="VFX94_08170"
     CDS             2459..>2705
                     /locus_tag="VFX94_08170"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX5768194.1"
                     /translation="MPAAAKEFGIVGLGRIGAGIAHHAIEKGMRVVGTSRSGAPRDLV
                     AAGLTEITGLSGFRAQLRPPRAILLYIPAGPAVDTTLD"
BASE COUNT          394 a          947 c          855 g          509 t
ORIGIN      
        1 gcttctcctt tacggcggca tggcgacgat cgcgtatggc ataagcggca tgctcgcctc
       61 ccagacgctc gggcgcctcg ccggcttcaa cctgctcgta tcctccggca ccctgctcgc
      121 ggccatcggc gccggccatg caggcgcgac gggcgcggcg ctgtactacc tcgtggtctc
      181 gaccctcggc agcgcggcgt tcttcctgct gatagagctc gtagagcgcg cgcgcacgcc
      241 gggtgcggac gtgctggcgg tgacggcggc ggcgttcggc ataggcggcg aagaaccgga
      301 accggagcac gaaaccggcg tcgccatccc cgcgagcatg gcgcttctcg ggcttagctt
      361 cgtctgctgc gcactccttg cggccggcct gccgccgctg ccggccttcg tcgcgaaatt
      421 cgcgctgctt gccgcgctcc tcggacccga tcccgtgccg ggagcggcgt gggcactgct
      481 cgtcctgctc acgctgtccg gcctcgccgc gatcatcgcc atgagccgct cgggagtgcg
      541 cattttctgg ggcgggacgg cgacaccgcc gcgtgtgcgc ctgatcgaaa tggcgccggt
      601 cgccgtgttg ctcgggctct gcttcgcctt gacggtggcc gcagggccag cgatggagta
      661 cctgagccgc accgcgcacg cgctgcacgc gcccggcggc tacatcgaaa gcgtgcttac
      721 gccatgacag gctggctacc ctatccgctt ctttcggtcg cgcttttcgc ggcctggctg
      781 ctgttgaatc aaagcttcgc cgccggtcac gtcgtccttg gcatcgcgct tggccttgcc
      841 ggcggtcgca tcctcgccgg gctccagccg ccacagcgcc gaccgcgccg cgccgccgtg
      901 gcgctcgagc ttttcttcct cgtgctcgcg gacatcgtgc gctccaacat cgcggtcgcc
      961 cgcatcgttc tcaatcccgg caccaagcgc accgccggct tcctgtcgct gcagctggag
     1021 ctacgccatc ccgccggcct tgcggtggtg gcgtgcatca tcaccgcgac gccggggaca
     1081 agctgggcgc gctacgacgc cgcgcgcaac atcgtcacca tccacgtgct cgaccttctc
     1141 gacgaggagg agtgggtcca cattttcaag cagcgctacg agcggcggct gcgggagatc
     1201 ttcgaatgaa cgccggtctg tcgggcgcca tactcattct cgcccaggtc ttcatcgcgc
     1261 tcgccatggc gtgcgcgctc taccgcctgc tgcgcggccc gcgcgcgcag gaccgcgtac
     1321 tcgcgctcga caccctctac gtgaacgcca tgctgctcgt gctgacgttc ggcatccgcg
     1381 aggccaccac cctctatttc gaggccgcgc tcgtcatcgg agtcatgggc ttcgtctcga
     1441 ccgcggcgct tgccaagttc ctgctccgcg gcgaggtgat cgaatgagca ccgccgccgc
     1501 cctcctcgtc cttgccggcg caagccttgc cctcatcggc tcgatcggcc tgctgcgcat
     1561 gaaaacgttc tacgaacgcg tgcatgcgcc caccatgggc accacgctcg gcacagggct
     1621 cgtgctcctc ggttcgatga ttttcttcac ctttcacgag tcgcgtccgg tgctgcacga
     1681 gattctcatc ggcgtcttca tgacgctcaa cacgccggtg acgtacatga ttctcgtgcg
     1741 cgccgcgctg catcgcgacc ctatcgacat cccaagatcc tgatgtatcg gcgcggcgat
     1801 ccgcgctaac atgcccgaag aggagcgcta ttacccgctg agcggaatgc tcatggtgct
     1861 gttcgggcgc ctgccgcaga cctccgatcg cgccgcctgg cagcactggc gtttgggggt
     1921 gctcgacatg gacggcaagc gggtggacaa agtgctcgcc acgcgccttc cgaacagccc
     1981 tccatcctga ggagcacaca tggcctggac cattctagtt atcgccggac tgttcgaggt
     2041 cggctgggcg atcgggttga agtacaccga agggttcacc cggttgtggc ccaccgtctg
     2101 gacggtcctc gcgatgatcg taagtctttg gttgctgggc gttgcgatga aatcgctgcc
     2161 cgtcggcacc gcttacagcg tttgggtggg cgttggtgcg gtcggcaccg taatactggg
     2221 catcgtgctg ctgggagagc ccgccaacgc agcgcggctg atcagcattg cattcatcgt
     2281 tgcgggcatc gtcggcttga agcttgcgac acccggctga cctacgaaga gcgagtgcca
     2341 gccaagtact ccgcgcttgt ggcgagcgtc tacttcaacc gacaaggatc gtggaagctc
     2401 gcgttccatc agcagacgcc ggggcgaact taacgccacg cgaagcgtcc gacaaatcat
     2461 gccagccgcg gccaaggaat tcggcatcgt cggcctgggc cgtatcggcg cgggcatcgc
     2521 ccatcatgcg atcgagaaag gcatgcgcgt ggtcggcacg agccgcagcg gcgcgccgcg
     2581 cgacctcgtc gcggcaggcc tcaccgagat caccgggctg agcggctttc gagcgcagct
     2641 gcgcccgccg agagcgattc tcctctacat acccgccggg cctgcggtcg atacgactct
     2701 cgatg
//
LOCUS       DASUNT010000403         5813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_933385, whole genome shotgun sequence.
ACCESSION   DASUNT010000403 DASUNT010000000
VERSION     DASUNT010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933385"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..635)
                     /gene="thiE"
                     /locus_tag="VFX94_08175"
     CDS             complement(36..635)
                     /gene="thiE"
                     /locus_tag="VFX94_08175"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX5768195.1"
                     /translation="MMRGLYPITPDTADTDALLRKVEAVLKAGAAMLQYRNKAISRDK
                     RLAQASELAGLMRRYGVPLIVNDDIDIALAVDASGAHIGRDDGDLAAARARLGGKILG
                     ASCYDSLESARAAVRAGADYVAFGSVFPSPTKPDARRAPLSLFRQELGVPLCAIGGIT
                     LDNAPAVIAAGASLLAVISDVFDAPDIGARAHQYGRLFS"
     gene            complement(632..1381)
                     /locus_tag="VFX94_08180"
     CDS             complement(632..1381)
                     /locus_tag="VFX94_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEX5768196.1"
                     /translation="MPPIVLTFAASDPTGGAGMQADLLTLAALGCHPLSVLTGLTVQD
                     TSGVELLEPVDAGLVTRQALCVLAESKVAAFKVGVLGSADNVRAVAQVVIAHAEVPLV
                     LDPVLASGRGDELASAAVRAALLECLVPRATVVTPNTLEAERLGGVTALLERGCRYVL
                     VTGSHEPGAEVTNTLYDPQGLVRADRWPRLHGSYHGSGCTLASAIAAQLAQGIAVSEA
                     VRAAQQFTWRALAAAFRSGGGQLMPNRFFAP"
     gene            1404..1589
                     /locus_tag="VFX94_08185"
     CDS             1404..1589
                     /locus_tag="VFX94_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX5768197.1"
                     /translation="MTENQVQYKTWMCLICGFVYDEAAGLPDEGIAPGTRWEDVPMNW
                     TCPECGARKEDFEMVEL"
     gene            1654..2214
                     /locus_tag="VFX94_08190"
     CDS             1654..2214
                     /locus_tag="VFX94_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX5768198.1"
                     /translation="MSSINLTHHFLIAMPNMADPYFAKTLTYICEHNDQGALGLVVNR
                     PIDMTLQALFERLSLTLRDAQRRDAPIYFGGPVQQDRGFVLHAPAGNWQATLRVRDVI
                     GLTTSKDILEAVGRGEGPARMLVTLGYAGWSPGQLEHELSQNAWLTVEARDAIIFDTP
                     AEERLPAAMELLGLDYARLQDAAGHA"
     gene            2207..2593
                     /gene="ruvX"
                     /locus_tag="VFX94_08195"
     CDS             2207..2593
                     /gene="ruvX"
                     /locus_tag="VFX94_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX5768199.1"
                     /translation="MPERGTVLAFDFGIKRIGVAVGEPELRTAHPLPAVSDFSQIQRL
                     VEQWKPASLVVGLPTSAQGEPHTMTRQAEGFARRLERRFKLPVARVDERYTSLEAAHR
                     LRGVKKKAIDSVAAQLILEQYFDQAA"
     gene            2577..3083
                     /gene="pyrR"
                     /locus_tag="VFX94_08200"
     CDS             2577..3083
                     /gene="pyrR"
                     /locus_tag="VFX94_08200"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX5768200.1"
                     /translation="MTRLPEAEELCAQLAAELGPRIGPNTAMVGLYTGGAWLAERLHA
                     MLGMESPLGLMDIAFYRDDYAARGLKHDPKRTRIPFDVSGRELLLVDDVLYSGRTVRA
                     AMNELFDYGRPASISLVVLVDRGGRQLPICAQHVGARVEVPAGMRLTLKRDASGKLAL
                     ALEPQRAA"
     gene            3049..4023
                     /locus_tag="VFX94_08205"
     CDS             3049..4023
                     /locus_tag="VFX94_08205"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX5768201.1"
                     /translation="MRLRWNRSVQRNPQLNEKGELQHLLSIEGLPREVLLQILDTAES
                     FVGVTEREVKKVPLLRGKSVFNLFFEPSTRTRTTFEIAAKRLSADVINLNITVSSATK
                     GESLLDTVANLSAMHADIFVVRHESSGAPFLIARHVKPHEHVINAGDGRHAHPTQGLL
                     DLFTIRHYKKDFTRLAVAIVGDILHSRVARSLIHGLTTLGVPEVRVIGPQTLLPTGVE
                     ALGVRVFHDMRAGLKDCDVVVMLRLQNERMRGALLPSAQEYFKHYGLTADKLSLARRD
                     AIVMHPGPMNRGVEIDSTVADGPHSVILPQVTFGIAVRMAVMSIVAGN"
     gene            4023..5282
                     /locus_tag="VFX94_08210"
     CDS             4023..5282
                     /locus_tag="VFX94_08210"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX5768202.1"
                     /translation="MRLHIRNARVVDPASGREGSGELFVVDGRISGEGGKPDRVIDAT
                     NLVVAPGFIDLSARLREPGYEYKATLESEMQAALAGGVTSLACPPDTDPPLDEPGLVD
                     MLRRRAKALSCARVYPIGALTMKLEGQALTEMGQLTDAGCVAFSQANAPLADTQVLWR
                     ALQYAATFGYPVWLRAEDAWLARGGVAHDGEVATRLGLAGIPPFAETIALATLLELVR
                     ATRARLHVCRLSTAGAVELMRRAKQEGLAVSCDVGVHHVHLCDMDLGYFDSQCRLEPP
                     LRAGRDRDALARALAEGVIDCLCSDHTPVDDDGKHLPFAQAEPGATGLELLLPLALKW
                     GEAQGLGLAATLARITSEPARILGVPSGRLTPGAPADLAIFDPAARLRIAPETLKSQG
                     KNTPFLGCELAGRVRYTIVAGQVVYES"
     gene            5279..>5813
                     /locus_tag="VFX94_08215"
     CDS             5279..>5813
                     /locus_tag="VFX94_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX5768203.1"
                     /translation="MSRVGLLALVLVLAGCGSTSRPGGYYLDDGPPERPPADLDRVAD
                     AVPRVEPLHKYANRPYNALGRDYVPMTSLQPFRQRGLASWYGKRYHGRNTSSGELYDM
                     YAMSAAHPTLPIPSYARVTNVANGRSVVVRINDRGPFRGQRIIDVSYVAAHKLGFIQA
                     GQAQVEVEAIVPGRTAGL"
BASE COUNT          947 a         1965 c         1988 g          913 t
ORIGIN      
        1 acgcccggcg cgctcgaaaa gatccttgct ttttgtcatg agaagagcct gccgtattgg
       61 tgcgcccgcg ccccgatgtc gggcgcgtcg aaaacgtcgc tgatgacagc aaggaggctt
      121 gcgccggccg cgattacagc cggcgcgttg tcgagcgtga tcccgccgat cgcgcaaagc
      181 ggcacgccga gctcctggcg aaaaagcgaa agcggcgcgc gcctcgcgtc gggcttggtg
      241 ggcgagggaa acacgctccc gaacgccaca taatcggcgc cggcgcgcac ggcggcccgg
      301 gcgctctcga ggctgtcgta gcaggaggcg ccaagaatct ttccgccgag ccgcgcgcga
      361 gcggccgcga gatcgccgtc atccctgcca atgtgcgcgc cgctcgcgtc cacggcgagg
      421 gcaatgtcga tgtcgtcgtt cacgatgagc ggcacgccat agcgacgcat gagcccggca
      481 agctcgcttg cctgcgcgag gcgcttgtcc ctggagatgg ccttgttccg gtactgcagc
      541 atggcggccc cggccttgag caccgcttcc actttgcgca gcagcgcatc ggtgtcggcc
      601 gtgtccggag taatcgggta gagcccgcgc atcatggtgc gaaaaagcgg ttcggcatga
      661 gctggccgcc gccgcttcgg aatgcggcgg cgagcgcccg ccacgtgaac tgctgcgccg
      721 cgcgcaccgc ttcagacacg gcgatgccct gcgcaagctg cgccgcgatc gcgctcgcga
      781 gggtgcatcc cgagccgtgg tagctgccgt ggagccgcgg ccaccggtcc gcgcgcacca
      841 gcccctgtgg atcgtaaagc gtattggtga cttccgcgcc cggctcgtgc gatccggtga
      901 caagcacgta gcgacagccg cgctcgagga gcgcggtcac cccgccaagg cgctcggctt
      961 ctagcgtgtt cggcgtgacg accgtcgccc gcggcacgag gcattccagc aatgcggcac
     1021 gcaccgcagc gctcgcgagc tcgtcgccgc gccccgaggc gagcaccggg tcgagcacca
     1081 agggcacctc tgcgtgcgcg atcacgacct gtgccaccgc gcggacattg tccgcagagc
     1141 cgagcacgcc gactttgaac gccgccacct tgctctcggc gagcacgcag agcgcctggc
     1201 gcgtgacgag gcctgcatcg accggctcga gcagctcgac cccgctcgtg tcctgaaccg
     1261 tgagcccggt cagcaccgaa agcggatgac agcccagggc ggcgagcgtc agcagatcgg
     1321 cctgcatccc cgcaccgccg gtgggatcgg acgcggcgaa cgtcagaacg atcggaggca
     1381 tgagttaata tccggcccat tttatgaccg agaaccaagt gcagtacaag acgtggatgt
     1441 gcctcatctg cggctttgtc tacgacgagg cggcgggact gcccgacgag ggcatcgcgc
     1501 cgggcacgcg ctgggaagac gtgcccatga actggacctg tcccgagtgc ggcgcgcgca
     1561 aggaagactt cgagatggtc gagctctgaa cggcgcaaac tttccgcgcg cggggcgatc
     1621 caagcccttt gacagtaaaa taagggcggg cggatgtcct cgatcaatct gacgcaccac
     1681 ttcctgatcg ccatgcccaa catggccgat ccgtatttcg ccaagacgct cacctacatc
     1741 tgcgagcaca acgaccaggg cgcgctcggc ctggtcgtaa accggccgat cgacatgacg
     1801 ctgcaggcgc tgttcgagcg gctatcgctt actttgcgcg atgcgcagcg cagggacgcg
     1861 ccgatctatt tcggcggccc ggtgcagcag gatcgcggct tcgtgctgca tgcgccggcg
     1921 ggcaactggc aggccacgct ccgcgtgcgc gacgtgatcg gcctcaccac gtccaaggac
     1981 atccttgaag cggtgggccg cggggagggc ccggcgcgca tgctggtcac gctcggctac
     2041 gcgggctggt cgcccggcca gctcgagcac gagctctcgc agaacgcctg gctcacggtg
     2101 gaggcgcgcg acgcgatcat cttcgatacc ccggcggagg agcgcctgcc ggcggcgatg
     2161 gaactcctcg gcctcgacta cgcgaggctg caggacgcgg cagggcatgc ctgagcgggg
     2221 cactgttctc gctttcgact tcggcataaa gcgtattggc gtagcggtag gggaaccgga
     2281 gctgcgcacg gcccacccgc ttccggcggt ttcggacttt tcgcagattc aaaggctggt
     2341 ggagcagtgg aagccggcga gcctcgtggt cggcttgccg acctccgcgc agggcgagcc
     2401 gcacacgatg acacgccagg ccgagggctt cgcgcggcgc ctggagcggc gcttcaagct
     2461 gccggtggcg cgggtggacg agcgatacac ctcgctcgag gcggcgcatc gcctgcgtgg
     2521 ggtgaaaaaa aaggcgatcg actcggtcgc ggcgcagctc attctggagc aatactttga
     2581 ccaggctgcc tgaagcggag gagctttgcg cgcagctcgc ggccgagctc gggccgcgca
     2641 tcgggccgaa caccgcgatg gtggggctct ataccggcgg cgcgtggctt gccgagcggc
     2701 tgcacgcgat gcttggcatg gaaagcccgc tcgggctgat ggacatcgct ttctaccgcg
     2761 acgactacgc ggcgcgcggc ttgaagcacg accccaagcg caccaggatc ccgttcgacg
     2821 tcagcggccg cgaacttctg ctggtggacg acgtgctcta cagcgggcgc acggtgcgcg
     2881 cggcgatgaa cgagctgttc gactacgggc gcccggcgag catatcgctc gtcgtccttg
     2941 tcgaccgcgg cggccggcag ctgcccatct gcgcgcagca cgtaggcgcg agggtagaag
     3001 tgccggccgg aatgcgcctc acgctgaagc gcgacgcgag cggaaagctt gcgcttgcgc
     3061 tggaaccgca gcgtgcagcg taatccccag ctcaacgaaa aaggcgagct gcagcacctt
     3121 ctttccatcg aagggctgcc gcgcgaggtc ctgctgcaga tcctcgatac cgcggagtcc
     3181 ttcgtcggtg tcaccgagcg cgaggtgaag aaggtgccgc tgctccgggg aaaaagcgtg
     3241 ttcaacctct ttttcgagcc gtccacgcgc acccgcacga cgttcgaaat cgccgcgaag
     3301 cgcctgtccg cggacgtcat caacctgaac atcaccgttt cttcggccac gaagggcgag
     3361 tcgttgctcg acaccgtggc gaacctctcg gcgatgcatg ccgacatctt cgtggtgcgg
     3421 catgagtcct ccggcgcccc gttcctgatc gcgcgccatg tgaagccgca cgagcatgtg
     3481 atcaacgccg gcgacgggcg ccacgcgcac ccgacgcagg ggctgctcga cctcttcacc
     3541 atccgccact acaagaagga cttcacgcgt cttgcggtgg caatcgtcgg cgacatcctg
     3601 cattcgcgcg tcgcgcgctc gctcatccac gggctcacta cgctcggcgt ccccgaggtg
     3661 cgggtgatcg gtccgcagac gctccttccc acgggcgtgg aagcgcttgg cgtgcgcgtc
     3721 ttccacgaca tgcgcgccgg gttgaaggac tgcgacgtcg tcgtcatgct gcgcctgcag
     3781 aacgaacgca tgcgcggtgc cttgcttcca tcggcgcagg aatacttcaa gcactatggg
     3841 ctcaccgcgg acaagctctc gctcgcgcgg cgcgacgcca tcgtcatgca cccggggccg
     3901 atgaaccgcg gcgtggaaat cgattccacc gtggcggacg ggccccactc ggtgatcctg
     3961 ccgcaagtga ccttcggcat cgcggtgcgg atggctgtga tgagcatcgt ggccggcaac
     4021 tgatgcggct tcatatcagg aacgcccggg tggtcgaccc cgcaagcggc agggaaggca
     4081 gcggagagct tttcgtcgtc gacggcagga tttccggcga gggcggaaaa cccgatcgcg
     4141 tcatcgatgc gacgaacctg gtcgtcgcgc ccgggttcat cgacctttcc gcgcggctgc
     4201 gcgagccggg ctacgagtac aaggcgacgc tcgagtcgga gatgcaggcc gcgctcgccg
     4261 gcggcgtgac gagccttgcc tgtccgccgg acaccgaccc gccgctcgac gagccgggcc
     4321 tcgtggacat gctgcgccgg cgagcgaaag cgctctcctg cgcgcgcgtc tacccgatcg
     4381 gggccctcac catgaagctc gagggccagg ccctcaccga gatgggccag ctcaccgacg
     4441 cgggctgcgt cgcgttttcg caggcgaatg cgccgctcgc cgatacgcag gtgctgtggc
     4501 gcgcgctgca atacgcggcg accttcggct atccggtgtg gctgcgtgcg gaggatgcgt
     4561 ggcttgcgcg gggcggcgtg gcgcacgacg gcgaggtggc aacgcggctc gggcttgccg
     4621 ggatcccgcc tttcgccgag acgatcgcgc tcgcgacact gctcgagttg gtacgcgcca
     4681 cgagggcgcg cttgcacgtg tgccggctat ccaccgcggg cgctgtggag ctcatgcgcc
     4741 gggcaaagca ggagggcctg gcggtgagtt gcgacgtcgg cgtccatcat gtgcatctgt
     4801 gcgacatgga cctcggctac tttgattcgc aatgccgcct tgagccaccg ctgcgcgccg
     4861 ggcgcgatcg cgatgccctt gcccgggcgc tggccgaagg cgtcatcgac tgcctttgct
     4921 cggatcacac gccggtcgac gacgacggca agcacctgcc gttcgcccag gcggagccgg
     4981 gcgcgaccgg gctcgagctc ctcctgccgc tcgcgctcaa gtggggcgag gcgcaagggc
     5041 tcggactggc ggcaacgctc gcccggatta cgtcggagcc ggcgcgcata ctcggcgtcc
     5101 cgtccggccg cctgaccccc ggcgcgcccg ccgatcttgc catcttcgac ccggcggccc
     5161 ggctgcgcat cgcgccggag acactgaaaa gccagggcaa gaacacgccg tttctcggct
     5221 gcgagctcgc cggccgggta cgctatacga tcgtggcagg ccaggtggtg tacgagtcat
     5281 gagcagagtt gggctcctcg cgctcgtgct cgttctcgcc ggttgcggct cgacctcgag
     5341 gcccggcggc tactacctcg acgacggccc gcccgagcgg ccgcccgccg acctcgaccg
     5401 cgtcgcggac gcggtgccgc gcgtcgagcc gctgcacaag tacgcgaacc ggccgtataa
     5461 cgcgctcggc cgcgactacg tgccgatgac ttcgctgcag ccgttccgcc agcgcgggct
     5521 cgcgagctgg tacggcaagc gctaccacgg caggaacact tccagtggcg agctctacga
     5581 catgtatgcg atgagcgcag cgcatccgac gctgccaatc ccgagctatg cccgcgtcac
     5641 gaacgtcgcg aatggcagga gcgtcgtggt gcgcatcaac gaccgcggcc cgttccgcgg
     5701 gcagcgcatc atcgatgttt cctacgtcgc cgcgcacaag ttgggcttca tccaggcggg
     5761 acaggcgcag gtcgaggtcg aagcgatcgt gccgggcaga acggcgggct tat
//
LOCUS       DASUNT010000404         3839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_408636, whole genome shotgun sequence.
ACCESSION   DASUNT010000404 DASUNT010000000
VERSION     DASUNT010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408636"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..555)
                     /locus_tag="VFX94_08220"
     CDS             complement(154..555)
                     /locus_tag="VFX94_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768204.1"
                     /translation="MDGAGTGLALARHNTRGEAIPMKLHTRERRSVFLVFLGACFVAL
                     MTALPIVTAQVEKEAMSKPSAAPAAAGGPSTDAPDFERADRNKDGIVDKSEAGVVPGL
                     SANFERADRNKDGKLDREEYAQGLRILQVRR"
     gene            complement(680..1711)
                     /locus_tag="VFX94_08225"
     CDS             complement(680..1711)
                     /locus_tag="VFX94_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5768205.1"
                     /translation="MVIDVHGHYTTEPAALLAFRDKQLAGLADPMRKPATNELNISDE
                     DLVRSVQPQIKLQKERGSDLTIFSPRAAGMAHHVGTEATGIEWSRMSNDLIQRICTLL
                     PANFAPVGQLPQHPGVAPKNCVPELERLAKLGFVGVNLNPDPAGGYWTDPPLTERWWY
                     PLYEKLCELDMTAMIHVTSSCNPAFHYTGAHYINGDTTAFMQLVQGDLFKDFPTLRFV
                     IPHGGGAVPFHWGRYRGLAQEMKKPLLRDHLLGNVFFDTCVYHLPGIELMLKVIPVDN
                     ILFASEMLGAVKGIDPETGHNYDDTKRYFDQVQGLSAEDRRKIFEGNARRVYPRLEAR
                     LRKTSLHAS"
     gene            complement(1782..2408)
                     /locus_tag="VFX94_08230"
     CDS             complement(1782..2408)
                     /locus_tag="VFX94_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX5768206.1"
                     /translation="MKRTALLAVLVLSLGVIGCSEMPSMPAWMPGSGWTTLIDGEKGL
                     ENFERVGDANWRAQDGAITADSGKGGFLLSKRSYKDFELRAEFWADHNANSGIYMRCS
                     DPKNLNDKSCYEANIFDQRPDQTYATGGIVHRGKVLKPVKAGGQWNVYEITAKGSRMT
                     VVLNGIKTAEIDNVESSSGPFALQYGTLPPKGAPGGAIRWRKVQVKEI"
     gene            2478..>3839
                     /locus_tag="VFX94_08235"
     CDS             2478..>3839
                     /locus_tag="VFX94_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5768207.1"
                     /translation="MNFRSWNRRAVAWVGGLFIAAIVALAAYDIVTSYQAAVATTGRE
                     LEAQARLIAEQTARSLQAVDLVLRHIGQQHQQGAFDRMSERQLHNYLREQSVGLVQIE
                     GLMLADSSGAPRASSLRYPLPETLVNVSGFGIFQALRQGHRGMYVDDAVKSPVDGIWN
                     VPLARRLDSADGRFAGAVGARGRIEYFEQFYRNAQLDPSTTIALMRDNGTLLARHPRV
                     ESAFGQRFALFDRFAAVNTGPIRDVSSIDGVERFAALKLVPDYPVAVVVTRDAAAALG
                     PWRAQSIGTALRTLALSALAALLLVVLLRQLGRVQASQERFALAVAGSNDGIVDWDVV
                     NDRMFSSRRAMQIVGIDSEVEVRTRAEWRDLVVYHPDDAPRMKRDLDDFLQGRTELRD
                     GEYRVLMPGGEYRWIRIRNTCVRDARGRALRVAGSVSDIDAYKRAEEALKLSEEQYRS
                     IFNA"
BASE COUNT          579 a         1281 c         1288 g          691 t
ORIGIN      
        1 ggcggcgtcg gtgccctcct cgatcagctg cggcagctgt ccctcgaggc tcggctcgtc
       61 gccgagctct cgcagccagc gcgcttcgcg cgcgaggcgc cgcgccgtgc gcggatcggt
      121 ggcggtcttc accaggtgta gctccaatgt catttatctg cgcacctgca atatccgcag
      181 gccctgggcg tattcctcgc ggtcgagctt gccgtccttg ttgcggtcgg cgcgctcgaa
      241 gttggcggaa agccccggca ccacgccggc ttccgacttg tcgacaatgc catccttgtt
      301 gcggtcggca cgctcgaaat cgggcgcgtc ggtcgatggt ccgcccgcgg ccgcaggggc
      361 tgccgaaggt ttactcatgg cttctttctc cacctgggct gtgacgatcg gcagcgccgt
      421 catcagcgcg acgaagcagg cgccgaggaa aaccaggaag acgctgcgcc gctcgcgagt
      481 atgcaatttc atgggaatgg cttcgccacg cgtgttgtgc cgagccagtg caagccccgt
      541 gccagcgcca tccaacaagc acttagcgcg cagccgtgcg cgccgatgcg tcgggtagcg
      601 ggaccacgtt taccgcgccg gcccgaagcg tcttgttttc agggctgttt cggcgtctcc
      661 gggccgcgac gtcgtcgcct tacgaggcgt gtaacgatgt cttcctcagc cgcgcctcga
      721 gccgcgggta gacgcgccgc gcgttgccct cgaaaatctt gcggcggtcc tcggcgctca
      781 gtccttgcac ctggtcgaag tagcgcttgg tgtcatcgta attgtgcccg gtctcggggt
      841 cgatgccctt caccgcgccg agcatctccg aggcgaacag gatgttatcc accgggatca
      901 ccttgagcat gagctcgatc ccgggcaggt ggtacacgca cgtgtcgaag aacacgttgc
      961 cgagcagatg atccctgagc agcggcttct tcatctcttg cgcgaggccg cggtacctgc
     1021 cccagtgaaa cggcaccgcg ccgccgccgt gcgggatgac gaagcgcagc gtcgggaaat
     1081 ccttgaagag gtccccctgg acgagctgca tgaaggcggt ggtgtcgccg ttgatgtaat
     1141 gcgcgccggt gtagtggaac gccggattgc acgacgaggt gacgtggatc atcgccgtca
     1201 tgtcgagctc gcagagcttc tcgtacagcg ggtaccacca gcgctcggtg agcggcgggt
     1261 cggtccagta gccgcccgcc ggatcgggat tgaggttcac gccgacgaag ccgagcttcg
     1321 cgaggcgctc cagttccggg acgcagttct tcggcgccac gcccgggtgc tgcggcagct
     1381 gccccaccgg cgcgaagttc gcgggcagca gcgtgcagat gcgctggatc aggtcgttcg
     1441 acatgcgcga ccactcgatc ccggtcgcct ccgtcccgac gtggtgcgcc atgccggcgg
     1501 cgcgcggcga gaagatggtg aggtcggagc cgcgctcctt ttgaagcttg atctgcggct
     1561 gaacgctcct gaccaggtct tcatccgaaa tgttgagctc attcgtcgcg ggcttgcgca
     1621 tcggatcggc aagcccggcg agctgcttgt cgcggaaggc gagcagcgcg gccggctcgg
     1681 tggtgtagtg gccgtggacg tctatcacca tggcagcggt agcaattcgg ggacggagcc
     1741 cgaatgagtt cgggctccgt ccccgaatct cgggtgccgt tctagatctc cttgacctgc
     1801 accttgcgcc agcggatcgc gccgcccggg gcgcctttcg gcggcagggt tccgtactgc
     1861 aacgcgaacg ggccgctcga agactcgaca ttgtcgatct ccgcagtctt gatgccgttc
     1921 agcaccaccg tcatcctgga tcccttggcc gtgatctcgt agacgttcca ctggcctccg
     1981 gccttcaccg gcttgagcac cttgccgcgg tgaacgatgc cgcccgtggc ataggtctgg
     2041 tcggggcgct ggtcgaagat gttcgcttcg tagcaggact tgtcgttgag gttcttcgga
     2101 tcggagcagc gcatgtagat gccgctgttc gcgttgtggt cggcccagaa ctccgcccgt
     2161 agctcgaaat ccttgtacga gcgcttcgaa agcaggaagc cgcccttgcc gctgtcggcc
     2221 gtgatcgcgc cgtcctgggc gcgccaattt gcgtcgccga cgcgctcgaa gttctcgagc
     2281 cccttctccc cgtcgatcag cgtggtccag ccgcttcccg gcatccaggc cggcatggat
     2341 ggcatctccg agcagccgat gacgccgagg ctcaacacaa ggactgcgag cagcgcggta
     2401 cgtttcatgg ttcctccggt cttggtgtgg cgacagtcta tttaaaacgg gcgccgtagt
     2461 gctagggtgc cgcctacatg aacttccgta gctggaaccg ccgcgcggtc gcctgggtgg
     2521 ggggcctgtt catcgccgcg atcgtggcgc ttgccgccta cgacatcgtg acgagctacc
     2581 aggcagcggt ggccaccacc gggcgggagc tcgaggcgca ggcgcgcctc atcgcggagc
     2641 agaccgcgcg cagcctccag gcggtcgatc ttgtgctgcg ccacatcggc cagcagcacc
     2701 agcagggcgc cttcgaccgg atgagcgagc ggcagctgca caactacctg cgcgagcagt
     2761 cggtcgggct ggtgcagatc gagggattga tgctggccga ttcgagtggc gcgccgcgcg
     2821 cctcgtcgct gcgctacccg ctacccgaaa cgctggtcaa cgtgtcgggc ttcggaattt
     2881 tccaggcgct ccgacagggc catcgcggca tgtacgtgga cgacgccgtg aagagcccgg
     2941 tggacgggat ttggaacgtg ccgctcgccc gacggctcga cagcgccgac gggcgcttcg
     3001 ccggagccgt aggcgcgcgc ggccggatcg agtacttcga gcagttctac cgcaacgcgc
     3061 agctcgaccc ctcgaccacc atcgcgctca tgcgcgacaa cggcacgctc ctcgcgcgcc
     3121 atccgcgtgt cgaatcggcc ttcggccagc gcttcgcgct cttcgaccgc ttcgcggcgg
     3181 tgaacaccgg gccgatccgc gatgtgagct cgatcgacgg cgtggagcgc ttcgcggcgc
     3241 tcaagctggt gcccgattac ccggtcgcgg tggtggtgac gcgcgacgcg gccgccgcgc
     3301 tcggcccctg gcgcgcgcag tcgatcggca cggcgctgcg cacgcttgca ctgagcgcgc
     3361 tcgccgccct tctgctggtc gttctgctgc gccagctcgg ccgcgtgcaa gcgagccagg
     3421 agcgcttcgc gctcgcggtc gcgggctcga acgacggcat cgtcgactgg gatgtcgtca
     3481 acgaccgcat gttttcgtcc aggcgcgcga tgcagatcgt cggcatcgat tccgaggtcg
     3541 aggtgcgcac gcgcgccgag tggcgcgacc tcgtcgtcta ccacccggac gatgcgccgc
     3601 gcatgaaacg cgacctcgac gacttcctcc agggacgcac cgagctgcgc gacggcgagt
     3661 accgcgtgct catgccgggc ggcgaatacc gctggatccg catccgcaac acctgcgtgc
     3721 gcgacgcgcg cggccgagcg ctgcgcgtcg cgggctcggt aagcgacatc gacgcctaca
     3781 agcgcgccga ggaggcgctc aagctgagcg aagagcagta ccgctccatc ttcaacgcc
//
LOCUS       DASUNT010000405         6292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_670889, whole genome shotgun sequence.
ACCESSION   DASUNT010000405 DASUNT010000000
VERSION     DASUNT010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670889"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFX94_08240"
     CDS             complement(<1..183)
                     /locus_tag="VFX94_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX5768208.1"
                     /translation="MLDTTRRVATPEGIELTLRLAGPVPRALAWSVDLALRLALVLLV
                     MTLAAPLRRAGWGVVLL"
     gene            complement(235..1494)
                     /gene="rho"
                     /locus_tag="VFX94_08245"
     CDS             complement(235..1494)
                     /gene="rho"
                     /locus_tag="VFX94_08245"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX5768209.1"
                     /translation="MHLSELKTNHVSQLVDMAVSNGIENASRMRKQDLIFALLKNQAK
                     KGESIYGDGTLEVLPDGFGFLRSPDTSYLASTDDIYVSPSQIRRFNLHTGDSIEGEIR
                     TPKDGERYFALVKVDKVNSEPPEAAKNKILFENLTPLHPDEMFKLERDIKAEENITGR
                     VLDIVAPIGKGQRGLIVSPPKAGKTVMLQHIAHALTANHPDVALIVLLIDERPEEVTE
                     MSRSVRGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALQKPKRFFGAARTVEEGGSLTIIATALIDTGSRMDDVIYEE
                     FKGTGNMEVHLDRRMHEKRIYPAINVNRSGTRREELLIDQPVLQKIWVLRKLLFPMDE
                     LEAAEFLIDKLKATKSNAEFFDSMRRG"
     gene            complement(1697..2032)
                     /gene="trxA"
                     /locus_tag="VFX94_08250"
     CDS             complement(1697..2032)
                     /gene="trxA"
                     /locus_tag="VFX94_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEX5768210.1"
                     /translation="MASTASSIQRVTDETFEPEVLKSETPVLVDYWAEWCGPCKVIAP
                     ILDEVAKEYEGRLKIAKLNVDENHVTAAKYLVKGIPTLMLFKNGNLEATRVGALSKSQ
                     LTAFLDSNI"
     gene            2121..2444
                     /gene="fdxA"
                     /locus_tag="VFX94_08255"
     CDS             2121..2444
                     /gene="fdxA"
                     /locus_tag="VFX94_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEX5768211.1"
                     /translation="MTYVVTETCIKCKYTDCVDVCPVDCFREGPNMLVIDPDECIDCT
                     LCVPECPVEAIFAEDDVPDSQKKFTALNAELSKIWKPIIERKPAPPDADAWAKAKDKK
                     HLLER"
     gene            2619..2861
                     /locus_tag="VFX94_08260"
     CDS             2619..2861
                     /locus_tag="VFX94_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX5768212.1"
                     /translation="MEQGRLAFTAVYLKSNHGYIGFIEELPGVNSHGRTLDEARETLQ
                     KLAAVVFDEERREAEELIAGKDVVRESFFVPIPRAA"
     gene            2964..4487
                     /locus_tag="VFX94_08265"
     CDS             2964..4487
                     /locus_tag="VFX94_08265"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEX5768213.1"
                     /translation="MAARSWRTEDWVAVYLGFFIIVLILAAYSGKWFDLAQLRPTFRW
                     TAEAQIASAAPGWRGQLQGIAADADAKGRKDLASAARNLDAALDKGERTAIDKAAGAL
                     AKAGGRNTVPGTLGSEIRAHASSTADKVFAAPNLTKVLIVGIAALVIGAAGFALIGGK
                     VGAFMVGFPVVFILAFLARVIAGNGFFVDYGVEYVIFALLLGLLISNTIGTPAWLRPS
                     VQTEYFIKTGLVILGAGLLFMEVIQAGVLGILQAVLVVLVVWYVCFWMARKLRVDDDF
                     AAMLSSSVAICGVSAAIATCGAIQGDKKKLSYVTSLVLIVAVPMMIVMPWIAKAFHFP
                     DMVAGAWLGGTLDTSASVVAAGALISDVAMKTGVIVKFSQNALIGVAAFILVVWWTYK
                     AGAQSKGQQPNAGIIWERFPKFVLGFLVASALFSFFLPAPLVEGTRSVLSELRTWWFA
                     LAFVCIGLETKFVDLATMEGGRPAVAFLGAQAVNIVWTLLLAYLLFGGVIFPLPDIK"
     gene            4495..5295
                     /locus_tag="VFX94_08270"
     CDS             4495..5295
                     /locus_tag="VFX94_08270"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX5768214.1"
                     /translation="MAWRVERQGRGSTPASTPGSVLIGAAHFFPRSFRRDLRRLIGEA
                     RIVLLEGPLDEEATRKVLAAGRGAGGGALYEAAKEEIDARLGIGRSPLDLHQLLKDLV
                     FGRQEQWLEEEMRALKPWAAFFGVWTRYRAREGWTHSLDLDAARIAAELAKETVYLET
                     IEEQIAALEAVPPERFVHFLAREDWSDYCARYERHYLAGDLDALVAAAQPFPTYCEPI
                     IGRRDPLLAARMAGELERGAACVVIGVAHCPGVLARLRELGYVYLPWP"
     gene            complement(5271..6221)
                     /locus_tag="VFX94_08275"
     CDS             complement(5271..6221)
                     /locus_tag="VFX94_08275"
                     /EC_number="7.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006119 - oxidative phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX5768215.1"
                     /translation="MGAAILAYVVLDGYDLGVGMLMPAATRDEQNRMVASIGPFWDAN
                     ETWLVLGIGLLLVAFPLAHGEILGALYLPVAAMLVGLMLRGVAFELRIKAEGWHRELW
                     NWLFWAGSALASLAQGFMLGRYITGFAPGMGYWLFALIVAASLCGGYVLLGATWLVLR
                     TDGPLQAKATVWARWGLVWVALGVALVSLATPLVSETVRAKWFDFPRTAALMVLPGGT
                     AAIWLWVWASLGRSDWKPFAGAVAIYVLAFMGLAYSLFPFVLIDRLTIWEAAAHPSAL
                     AFVLIGTLIVLPFIIGYTIYAYRVFRGKAQADDYGHGKYT"
BASE COUNT         1049 a         2004 c         2096 g         1143 t
ORIGIN      
        1 aaggagcacc acgccccagc cggcgcgcct gagcggcgcg gcgagcgtca tcaccaggag
       61 cacgagcgcc aggcgcagag cgaggtccac cgaccacgcg agcgcgcgcg gcaccggccc
      121 ggcgaggcgc agcgtcagct ctatgccttc gggtgtcgcg acacggcgcg tcgtgtcgag
      181 catcccttgt tcccccagaa caaatcgggg tcacaccccg attttctttt tacctcaacc
      241 tcgcctcatc gaatcgaaga actcggcgtt cgacttcgtc gctttcagct tgtcgataag
      301 gaattcggcg gcctcgagct cgtccatcgg gaagaggagc ttgcgcagga cccagatttt
      361 ctgcagcacc ggctggtcga tcagcagctc ctcgcgccgc gtgccggagc ggttcacgtt
      421 gatggcggga tagatgcgct tctcgtgcat gcgccggtca aggtggacct ccatgttgcc
      481 ggtgcccttg aattcctcgt agatcacgtc gtccatgcgg ctgccggtgt cgatgagcgc
      541 ggtggcgatg atggtgagcg agccgccctc ctcgacggtg cgcgcggcgc cgaagaagcg
      601 cttgggcttc tgcagcgcgt tggcgtcgac gccgccggtc agcacttttc cggacgccgg
      661 aacgaccgtg ttataggcgc gcgccagccg ggtgatggaa tccaggagaa tcaccacgtc
      721 cttcttgtgc tcgaccaggc gcttcgcctt ctcgatcacc atctccgcga cctgcacgtg
      781 gcgcgaggcg ggctcgtcga atgtggaggc gaccacttcg ccgcgcaccg agcgcgacat
      841 ctcggtcact tcctccggcc gctcgtcgat gaggagcacg atcagcgcca cgtcgggatg
      901 gttggcggtg agcgcgtgcg cgatgtgctg gagcatgacg gtcttgcccg ccttcggcgg
      961 gctgacgatg aggccgcgct ggcccttgcc gatcggggcc acgatgtcga gcacgcgccc
     1021 ggtgatgttt tcctcggcct tgatgtcgcg ctcgagcttg aacatctcgt ccgggtgcag
     1081 cggcgtgagg ttctcgaaca ggatcttgtt cttggccgct tccgggggct cgctgttgac
     1141 cttgtccacc ttgacgagcg cgaagtagcg ctcgccgtcc ttcggcgtgc ggatctcgcc
     1201 ttcgatcgag tcgccggtat gcaggttgaa gcgccggatc tgcgacggcg agacgtagat
     1261 gtcgtcggtg ctcgcgaggt aggaggtgtc cggcgagcgc aggaagccga acccgtcggg
     1321 cagcacttcc agcgtgccgt cgccgtagat cgactcgcct ttcttcgcct ggttcttgag
     1381 cagcgcgaag atcaggtcct gcttgcgcat gcggctcgcg ttctcgatgc cgttcgacac
     1441 ggccatgtcg acgagctggg agacgtggtt ggtcttcagt tctgagagat gcataggtat
     1501 gggaatcggc gaaggcgacg cggcgcccgc ctgcgtttcc gggctggggg atgaacgaac
     1561 ttgttccggc tggactgcct ggggcctgct gcgcattctc gggaagtgcg ccccggattt
     1621 tgtcttgggc gctggacaac ctgttggaaa ccggccccgc taaacgcagg gcctacggcg
     1681 gcaaagccta aaagcttcag atgttgctgt caaggaaagc ggtgagctgg gacttggaca
     1741 gcgcgccgac gcgcgtcgct tcgaggttac cgttcttgaa cagcatcagg gtcgggatac
     1801 ccttcacgag gtactttgcc gccgtgacgt ggttctcatc gacattgagc ttcgcgatct
     1861 tcaggcggcc ctcgtactcc ttcgccactt cgtcgaggat cggcgctatc accttgcacg
     1921 ggccgcacca ctcggcccaa taatcgacga gcaccggggt ctcggacttc agcacctcgg
     1981 gctcgaaggt ttcgtcggtt acgcgctgga tggaactcgc ggtgctcgcc atgggtcctc
     2041 ggggattaat gcacggaaga gctctatatt atgggggcgc ccccggcgct tcccaagtga
     2101 atgataaact cttgatttca atgacctacg tagtcaccga gacgtgcatc aagtgcaagt
     2161 acaccgactg cgtcgacgtg tgcccggtcg actgctttcg cgaggggccc aacatgctgg
     2221 tcatcgaccc cgacgagtgc atcgactgca cgctgtgcgt tcccgagtgc ccggtggagg
     2281 cgatcttcgc cgaggacgat gtgccggact ctcagaaaaa attcacggcg ctgaacgccg
     2341 agctctccaa gatctggaag ccgatcatcg agcgcaagcc ggcaccgccg gacgcagatg
     2401 cctgggcgaa agcaaaagat aaaaagcatc tgctcgagcg atgaactcgc acgcagtgca
     2461 gcaatcgcac tgcgcacgtt tgcgcgccgc aaaaagtgtc gctgagacga ccacagcggt
     2521 tttgaaatat caatccgcag gaattttcta aagcaaagtc atgttcttga aagcttgatc
     2581 gcgtaacctg tgcatgctca ggcaggaggt tttcgcgaat ggaacaagga aggctcgcct
     2641 ttaccgccgt gtacctcaag tcgaatcacg gctacatcgg gttcatcgag gaattgcccg
     2701 gcgtgaactc gcatggccgc acgctcgatg aagcgcgcga gacgttgcag aagctcgctg
     2761 cggtggtgtt cgacgaggag cgccgcgagg ccgaggaact gatcgccggc aaggacgtcg
     2821 tgcgagagtc gttcttcgtg ccgattcccc gggccgcctg aggttattcc cgaagcgtga
     2881 aagggcgcct gcgggcgccc tttttgtttg aggggtaaca tcgcgccagc tggttgaagg
     2941 cggctaccga cagggggcta ggcatggcag ccaggagctg gcgcacggaa gactgggtcg
     3001 cggtctatct cggtttcttc atcatcgttc tcattctcgc ggcgtacagc gggaagtggt
     3061 ttgacctggc gcagctgcgg ccgacgttcc gctggaccgc ggaggcgcag atcgcttccg
     3121 ccgcgcccgg ctggcgcggg caactccagg gcattgccgc cgacgcggac gcgaagggca
     3181 ggaaagatct ggcgagcgcg gcgcgcaatc tcgatgcggc gctcgacaag ggcgagcgca
     3241 cggcgatcga caaggcggcc ggcgcgctcg ccaaagcggg cggacgtaac accgtgccgg
     3301 gcacgctggg ctccgagatc cgcgcgcacg cctcgtccac cgccgacaag gtgttcgccg
     3361 cgccgaacct cacgaaggtg ctcatcgtcg gcatcgcggc gctggtgatc ggcgcggccg
     3421 gtttcgcgct catcggcggc aaggtgggcg cgttcatggt cggctttccg gtcgtgttca
     3481 tcctcgcatt tcttgcgcgc gtgatcgccg gcaacggctt ctttgtagac tatggcgtcg
     3541 agtacgtcat cttcgcgctg ctgctggggc tgctcatcag caacacgatc ggcacgccgg
     3601 cgtggctcag gccctcggtg cagaccgagt acttcatcaa gaccggcctc gtgatcctcg
     3661 gtgcgggcct gctcttcatg gaagtcatcc aggcgggcgt gctcggcatc ctgcaggccg
     3721 tgctcgtggt cctggtcgtc tggtacgtgt gcttctggat ggcgcgcaag cttcgcgtgg
     3781 atgacgactt cgccgcgatg ctgtcctcgt cggtcgcgat ctgcggcgtg tccgcagcga
     3841 tcgcaacctg cggcgccatc cagggcgaca agaagaagct ttcctacgtc acctcgctcg
     3901 tgctgatcgt cgccgtgccg atgatgatcg tgatgccgtg gatcgccaag gctttccatt
     3961 tcccggacat ggtcgccggc gcgtggctcg gcggcacgct cgacaccagc gcgtcggtgg
     4021 ttgcggcggg agcgctcatc agcgacgtcg cgatgaagac cggggtgatc gtcaagtttt
     4081 cgcagaacgc gctgatcggc gtagcggcct tcatcctggt cgtctggtgg acctacaagg
     4141 cgggggcaca gtccaaggga cagcagccga atgccggcat catctgggag cgcttcccca
     4201 agttcgtgct cggcttcctg gtcgcctcgg cgctcttctc gttcttcctg cccgccccgc
     4261 tggtggaggg cacgcgctcg gtgctctcgg agctgcgcac ctggtggttc gcgctcgcgt
     4321 tcgtgtgcat cggcctggag accaagttcg tcgacctcgc cacgatggag ggcgggcgcc
     4381 cggcagtcgc attcctgggc gcgcaggcgg tcaacatcgt gtggacgctg ctgctcgcct
     4441 acctgctctt cggcggcgtc attttcccgc tgcccgatat caagtgacct gaaaatggcg
     4501 tggagggtcg agcgccaggg ccgcggctcg actcctgctt cgacccccgg gtcggtcctc
     4561 attggggcgg cgcatttctt tccgcgcagc ttccgccggg acctgcgccg gctgatcggc
     4621 gaggcgcgca tcgtgctcct cgaaggcccg ctcgacgagg aggcgacccg aaaagtgctc
     4681 gccgccggcc gcggtgccgg cgggggcgcc ttgtatgagg cagcaaagga ggaaatcgac
     4741 gcgagactcg gtatcgggcg ctcgccgctt gacctgcacc agcttctgaa ggatctcgtc
     4801 ttcggccgcc aggagcaatg gctggaggag gagatgcgcg cgctcaaacc gtgggcggcc
     4861 ttcttcggtg tctggacacg ctaccgggcg cgggagggct ggacccattc gctcgacctg
     4921 gacgccgcgc gtatcgccgc cgagctcgcg aaggagaccg tctacctcga aacgatcgag
     4981 gagcagatcg ccgcgctcga agcggtgccg ccggagcggt tcgtgcattt cctggcacgc
     5041 gaggactggt ccgactattg cgcgcgctac gagcggcact acctggccgg ggatctggac
     5101 gcgctggtcg cggcagcgca gccctttccc acttactgcg agccgatcat cgggcggcgt
     5161 gacccgctgc ttgcagcgcg catggcggga gagctcgagc gcggcgccgc ctgcgtcgtg
     5221 atcggcgtgg cccactgtcc gggcgtgctg gcgcggctgc gtgagctcgg ctacgtatac
     5281 ttgccgtggc cgtagtcgtc ggcctgtgcc ttgccgcgga acacgcggta ggcgtaaatc
     5341 gtgtagccga tgatgaacgg caacacgatc agcgtgccga tcagcacgaa cgccagagcg
     5401 ctcgggtgcg ccgccgcctc ccagatcgtc aggcggtcaa tcagcacaaa cgggaacagg
     5461 ctgtaggcga gccccatgaa cgcaagcacg tagatcgcca ccgcgccggc gaagggcttc
     5521 cagtccgatc tgccgaggct tgcccacacc cagagccaga tcgcggccgt gccgccggga
     5581 agcaccatga gcgccgctgt gcgcgggaaa tcgaaccact tcgcccgcac ggtctcgctc
     5641 acgagcggcg tggcgaggct gacgagcgct acgccgagcg ctacccacac caggccccag
     5701 cgcgcccaga cagtggcttt ggcctggagg gggccgtcgg tgcgcaacac gagccaggtc
     5761 gcgccgagca gcacgtagcc gccgcacagg cttgcggcga cgatcagcgc gaacagccag
     5821 taccccatgc ccggagcgaa gccagtgatg tagcgcccga gcatgaagcc ctgcgcgaga
     5881 cttgcgagcg cggaccccgc ccagaagagc cagttccata gctcccggtg ccagccctcg
     5941 gccttgatgc gcagttcgaa ggccacgccg cgcagcatga gcccgaccag catcgccgcc
     6001 accggcagat agagcgcgcc gaggatctcc ccgtgcgcga gcggaaacgc gacgagcagc
     6061 agcccgatcc cgagcacgag ccaggtttcg ttcgcgtccc agaaggggcc gatcgaggcc
     6121 accatgcggt tctgctcgtc gcgggttgcc gccggcatca gcatgccgac gccgagatcg
     6181 tagccatcga gcaccacgta ggcgaggatc gccgcgccca tgaggatcgt gaacaccagc
     6241 ggcagatcga tcacgacgcg cctttccccg cgaggtgggt cagcaccacc at
//
LOCUS       DASUNT010000406         3819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_846707, whole genome shotgun sequence.
ACCESSION   DASUNT010000406 DASUNT010000000
VERSION     DASUNT010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846707"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..448)
                     /gene="apaG"
                     /locus_tag="VFX94_08280"
     CDS             complement(65..448)
                     /gene="apaG"
                     /locus_tag="VFX94_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX5768216.1"
                     /translation="MAREKLYEIEVNAATQYLADQSDESAGRYVFAYTITLHNTGAVA
                     AQLVSRHWVITDAQGLVQEVRGLGVVGAQPLLQPGERYEYTSGASIATPVGTMRGSYQ
                     MVATDGTRFEAPIPEFTLSVPRVLH"
     gene            475..1143
                     /gene="rpe"
                     /locus_tag="VFX94_08285"
     CDS             475..1143
                     /gene="rpe"
                     /locus_tag="VFX94_08285"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX5768217.1"
                     /translation="MTFRIAPSILAADFSRLGDEVRAVERAGADLIHFDVMDNHYVPN
                     LTVGPLVCAALKPHVRLPLDVHLMVKPVDALVPSFAQAGASIISFHPEASEHVDRTIA
                     LIKEQGCKAGLVLNPATPVSWLDHTLDKVDMVLLMSVNPGFGGQQFIASVLPKIAEVR
                     RRIDASRKDIWLEVDGGIKTDNIAEVARAGADTFVAGSAIFGSKDYAATIRDMRQRLA
                     SAKL"
     gene            1140..2615
                     /gene="trpE"
                     /locus_tag="VFX94_08290"
     CDS             1140..2615
                     /gene="trpE"
                     /locus_tag="VFX94_08290"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX5768218.1"
                     /translation="MTDYTRVPVVAEARADFYTPLAVYLKLANTPYSYLLESVVGGER
                     FGRYSFIGLACAERIEARGRTVRRLLRDARGADVCLESVEADAFEYARAWLARHRVAP
                     VPAALRFGGGLAGYFGYEAVRHIEKSALKGEKPDPLGTPDMLLLVSDELAVMDNVLGK
                     LYLVVYGDPREPDAMRNAKSRLEHLRSRLAGPLPDERFFEEGKPRPASLECSFTEAQF
                     LEAVRRCKDYILAGDVMQVQVSQRTSRAFDAPPLELYRALRGVNPSPYMYYFDFGDHH
                     VVGASPEILVRLAGGTVTLRPIAGTRPRGATPEADAASAEELLADPKERAEHVMLIDL
                     GRNDVGRVAATGTVKVTERMVIERYSHVMHIVSNVEGRLAPGLDAIDVLKASFPAGTV
                     TGAPKVRAMQIIDELEPVKRGVYSGAVGYLGFNGDMDVAIAIRTAVVKDRRLNVQAAA
                     GIVADSDPRSEWLETQHKARALLRAAEIAQFDTAAGQPTQN"
     gene            complement(2597..>3819)
                     /locus_tag="VFX94_08295"
     CDS             complement(2597..>3819)
                     /locus_tag="VFX94_08295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5768219.1"
                     /translation="LALSNPSGALAEPLLLRLLQDTLIRDAELERILVEARRRMLEAP
                     LPLAVMVAIAHQCFNTEYVFDETPEERRRVSELRPADPSSYAVYASYRPLASLEQVNR
                     SGIDSLARRQIDEPAEERRLARTIAALRGTEDPVSLEVKAQYEENPYPRWIRTQAVFD
                     PAAPAGSPRILVAGCGTGQHAVTTALRYPGGTVLAVDLSLASLAYAKRKSAELRVANI
                     EYRQADILTLGTLEERFDIIECSGVLHHMAQPFEGWRVLASLRKPGGRMRIGLYSEAG
                     RGQVVRARELIARHDFKPDPDGIRAARRLIRKDPLLAQLSRNEDFYSMSGCRDLLFHV
                     HEQRFTLLEVESMIARLGLKFLGFELADSGASARRYAERFRGDPGGLDLANWHRLEQD
                     FPDTFSRMYQFWVG"
BASE COUNT          625 a         1262 c         1292 g          640 t
ORIGIN      
        1 tcgccgcttt catcgtgtgg ttcacctggc cgcggaagcg caaatgaagg ctcgtcgttt
       61 cagttcagtg gagcacccgc ggcacggaaa gcgtgaactc cggaatgggc gcttcgaagc
      121 gtgttccgtc ggtcgccacc atctggtagc tgccgcgcat cgtgccgacc ggcgtggcga
      181 tcgaagcgcc gctcgtgtac tcgtagcgct cgccgggttg cagcagcggc tgcgcgccga
      241 cgacgccgag gccgcgcacc tcctgcacca agccctgcgc gtccgtgatg acccagtggc
      301 ggctcacgag ctgggcggcc actgcgcccg tgttgtgcag cgtgatcgta taggcgaaga
      361 cgtagcgccc ggcgctctcg tcggattgat cggcgagata ctgagtcgcc gcgtttacct
      421 cgatctcgta gagcttctcg cgcgccacgg cggcattgta aactccgcgc cgccatgacg
      481 ttccgcatcg cgccctccat cctggcggcc gatttttccc ggctggggga cgaggtgcgc
      541 gcagtggagc gcgccggcgc ggacctgatc cacttcgacg tcatggataa ccattacgtg
      601 ccgaacctga cggtgggccc gctcgtctgc gcggcgctca agccgcatgt acggctgccg
      661 ctcgacgtgc atctcatggt gaagccggtg gacgcgctcg tgccctcgtt cgcccaggcg
      721 ggtgcgagca tcatcagctt tcatcccgaa gcttcggagc acgtggaccg caccatcgcg
      781 ctcatcaagg agcagggctg caaggcaggg ctcgtgctca atcccgccac gcccgtgtcg
      841 tggctcgacc acaccctcga taaggtcgac atggtgctcc tcatgtcggt aaatcccggc
      901 ttcggcgggc agcagttcat cgcctcggtg ctgccgaaaa tcgcggaggt gcgccgccgg
      961 atcgatgctt cgcgcaagga tatctggctc gaggtcgacg gcgggatcaa gaccgacaac
     1021 atcgcggaag tcgctcgcgc cggcgccgat accttcgtcg ccgggtccgc aattttcggg
     1081 tccaaggact acgcggcgac catccgggat atgcgccagc gcctcgcatc ggcaaagctt
     1141 tgaccgacta cacgcgggtc ccggtggtgg cggaggcgcg ggcggatttc tacactccgc
     1201 tcgcggtcta cttgaagctc gcgaatacgc cgtactccta tctgctcgag tctgtggtcg
     1261 gcggcgagcg gttcgggcgc tactcgttca tcggccttgc atgcgccgaa cgcatcgagg
     1321 cacgcggccg gacggtgcga cggctactgc gcgatgcgcg cggcgccgac gtctgcctcg
     1381 aaagcgtgga ggccgacgcg ttcgagtacg cgcgcgcctg gctcgcgcgc catcgcgtcg
     1441 ctcccgtgcc ggcggcgctg cgcttcggcg gcgggctcgc cggctacttc ggctacgagg
     1501 cggttcgcca tatcgaaaaa agcgcgctca agggagaaaa acccgatccg ctcggcacgc
     1561 cggacatgct gcttctcgtc tccgacgagc tcgcggtgat ggacaacgtg ctcggcaagc
     1621 tttacctggt ggtatatggc gatccgcgcg agcccgatgc aatgcgcaat gcaaaatcgc
     1681 ggctcgagca cttgcgttcc cgtctcgccg gaccattgcc cgacgagcgc tttttcgaag
     1741 aaggaaaacc acgccccgcg agcctggaat gctcgttcac ggaagcacag tttctggaag
     1801 ccgtacgccg ctgcaaggac tacatcctcg ccggcgacgt catgcaggtg caggtctccc
     1861 agcgcacctc gcgggcgttc gatgcgccgc cgctcgagct ctaccgggcg ctgcgcggcg
     1921 tgaacccctc gccctacatg tactactttg acttcggcga tcaccacgtg gtcggggcat
     1981 cgcccgagat tcttgttcgg ctcgcgggcg gcacggtaac cctgcggccg atcgccggga
     2041 cgcggccccg cggcgcgact cccgaagcgg acgcggcgag cgccgaagag ctgctcgccg
     2101 atcccaagga gcgcgccgag cacgtgatgc tgatcgacct cgggcgcaac gacgtcggcc
     2161 gggtcgcggc gaccgggacc gtcaaggtta ccgagcgcat ggtgatcgag cgctactcgc
     2221 acgtcatgca catcgtctcg aatgtcgagg gtcggctcgc gcccgggctc gatgcgatcg
     2281 atgtcctcaa ggcaagcttc cccgcgggca cggtgaccgg ggcgccgaag gtgcgcgcca
     2341 tgcagatcat cgacgagttg gagccggtca agcgcggggt ctatagcggc gcggtcggct
     2401 acctcggctt caatggcgac atggacgtgg cgatcgcgat ccggaccgcg gtggtgaaag
     2461 accggcgctt gaacgtgcag gctgcggccg gaatcgtggc ggactccgat ccgcgctcgg
     2521 aatggctcga aacgcagcac aaggcgcgtg ccctgctgcg ggcggccgaa atcgctcagt
     2581 tcgacaccgc ggccggtcag ccgacccaga actgatacat gcgggagaac gtgtccggga
     2641 agtcctgctc aaggcggtgc cagttcgcga gatcgagccc gccgggatct ccgcgaaagc
     2701 gctcggcata gcggcgcgcg ctcgcgccgc tgtccgcaag ctcgaagccg aggaacttca
     2761 agccgagccg cgcgatcatc gactcgacct cgagaagagt gaagcgctgc tcgtgcacgt
     2821 ggaacagcaa gtcccggcag ccgctcatgc tgtagaagtc ctcgttacga gagagttgcg
     2881 cgaggagcgg gtccttgcgg atcagcctgc gcgcggcgcg gatgccatcc gggtcgggct
     2941 tgaagtcgtg cctggcgatc aactctcgcg cgcgcaccac ctgcccgcgc ccggcttcgc
     3001 tgtagaggcc gatgcgcatg cggccgcccg gcttgcgcag cgacgcgagc acgcgccagc
     3061 cctcgaaggg ctgcgccata tgatgcagca cgcctgagca ttcgatgatg tcgaagcgct
     3121 cctcgagcgt gccgagcgtc aggatgtccg cttgcctgta ttcgatgttt gccactctca
     3181 gctcggccga cttgcgcttc gcgtacgcga ggctcgcgag gctgaggtcg accgcgagca
     3241 cggtgcctcc cgggtagcgc agcgcagtgg tcacggcatg ctggcccgtg ccgcagccgg
     3301 cgaccaggat gcgcgggctg cctgccggcg cggccggatc gaacaccgcc tgcgtacgaa
     3361 tccagcgcgg gtaggggttc tcctcgtact gcgccttcac ctccagcgaa accgggtcct
     3421 cggtgccgcg aagggcggca atagtgcggg cgaggcgcct ctcctcggcg ggctcgtcga
     3481 tctgccggcg agcgagcgaa tcgatgcccg agcgattaac ctgctccagg ctggcgagcg
     3541 gccgatagga cgcatagacg gcataagagc tcggatcggc gggccgaagc tcggatacac
     3601 gccggcgttc ctccggcgtt tcgtcaaaca catactcggt gttgaagcac tggtgcgcga
     3661 tcgcgaccat cacggcgagc gggagcggtg cttccagcat tcgccgtcgc gcttcgacga
     3721 ggatgcgttc gagctcggca tcacggatca aggtgtcctg caggaggcgc aggagaagcg
     3781 gctcggcgag cgcgccgctc ggattggaaa gcgcgagcg
//
LOCUS       DASUNT010000407         3051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_583205, whole genome shotgun sequence.
ACCESSION   DASUNT010000407 DASUNT010000000
VERSION     DASUNT010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583205"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VFX94_08300"
     CDS             complement(<1..967)
                     /locus_tag="VFX94_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5768220.1"
                     /translation="MRLGIFMMPVHPLHRNHTETLKEDRETVIFADQLGFHDAFIGEH
                     LTDKAENITNSLLFLATLIHSTKRIKLASGTCNLSQIHPTVVAANAAMFDHLAEGRFI
                     LGVSPGALVSDAEALGIMDEDRNKMFAEAIDVILEIWSREPPYEIDLPGNRFKVTTKK
                     TMHLETGVGIMPKPFQKPRPEIVGTVVAPFSKGVVLMGERDFHPLSANFLLPHWLKTH
                     WANYAEGKTKAGVKPDPAEWRVARTVFVADDDKVAERYGRTDPNSPYRYYWKILLKKM
                     MLSKRHVIFKTHEAQDDRELTEDYMVERLVICGSVNKVVDDILRLR"
     gene            complement(1004..1327)
                     /locus_tag="VFX94_08305"
     CDS             complement(1004..1327)
                     /locus_tag="VFX94_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768221.1"
                     /translation="MTPALDPVVVMALGGIVFVVAAYCALETWLRAWEQQVPSPLVRT
                     LYRIGANPERLAKPEVRRVIALAERICPACTATGACRESLESGSRMAYRSFCPNARLV
                     ERLTR"
     gene            complement(1324..1635)
                     /locus_tag="VFX94_08310"
     CDS             complement(1324..1635)
                     /locus_tag="VFX94_08310"
                     /inference="COORDINATES: protein motif:HMM:NF040427.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6455 family protein"
                     /protein_id="HEX5768222.1"
                     /translation="METFIFLALFGLLVAAVLPALYALNRRMSARSGELEIWKVMHRA
                     GVSPADAAAEQPRALAAALRRCALCPSVDACHEWLASSSRAGLERFCPNAAYFRKLEE
                     R"
     gene            1701..>3051
                     /gene="mnmD"
                     /locus_tag="VFX94_08315"
     CDS             1701..>3051
                     /gene="mnmD"
                     /locus_tag="VFX94_08315"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HEX5768223.1"
                     /translation="MQQALVPAKLAFRDGTPYSAAYGDVYHSAAGGPAQARHVFLGGN
                     SLPGRWAGRSRFVILETGFGFGLNFLATWQAWRLDPRRCARLHYVSLEKHPFTQADLK
                     LAHDHYPEFALEAAQLQACWPVLVPGAHRLELDGGRVVLTLFFADVALARDLRLAADA
                     YYLDGFAPAKNPDMWSPQIMRALSRLAAPGATAATWSVASAVRASLEATGFAVHKRVG
                     FGDKREMLTATSIREGDGFAPLQRQAAVIGAGIAGAAVCERLCARGWEVELYERRTAP
                     AQEASGNHAGAFHPIVTPDDSVFARITRAAFLYSTRRWSLLENLRWDPCGVLQLARDA
                     KEEASQRASVGALALPPEYAQYVTREEASAHAGVPVAAPGLWFPQAGWTRPQSLVNAL
                     LAACGSRLNTSFATEVSRLPETPVVVLANSGEAPKLHAPPHLRLRKVRGQVTHVPASA
                     "
BASE COUNT          485 a         1008 c         1019 g          539 t
ORIGIN      
        1 cgcgcaggcg caggatatcg tccaccactt tgttgacgct gccgcagatc accaggcgct
       61 cgaccatgta gtcctcagtg agctcacgat cgtcctgcgc ttcgtgcgtc ttgaaaatga
      121 cgtggcgctt cgagagcatc attttcttca gcaggatttt ccagtagtag cgatacgggc
      181 tgttcgggtc cgttcggcca taacgctcgg cgactttgtc gtcgtccgcg acgaacacgg
      241 tgcgcgcgac gcgccattcc gccgggtcgg gcttcacgcc ggctttcgtc ttgccctccg
      301 cgtagttcgc ccagtgggtc ttcagccagt gcggcagcag gaagttcgcc gacagcgggt
      361 ggaaatcgcg ctcgcccatg agtaccacgc ccttggagaa cggcgcgacc acggtgccga
      421 cgatctcggg gcgcggcttc tggaagggct tgggcatgat gccgacgccg gtctcgaggt
      481 gcatggtctt cttcgtcgtc accttgaagc ggttccccgg caggtcgatc tcgtagggcg
      541 gctcgcgcga ccagatctcg aggattacgt cgatcgcctc ggcgaacatc ttgttgcggt
      601 cttcgtccat gatgccgagc gcctcggcgt cggacacgag cgccccggga ctcacgccga
      661 ggatgaagcg gccctcggcc aggtggtcga acatcgccgc gttggccgcc acgacagtcg
      721 ggtggatctg ggacagattg caggtgccgc tcgcgagttt gatgcgcttc gtcgaatgga
      781 tcagcgtcgc gaggaaaaga agcgagttcg tgatgttctc ggccttgtcg gtgaggtgct
      841 cgccgatgaa cgcgtcatgg aagccgagct ggtcggcgaa gatcaccgtc tcgcggtctt
      901 ccttgagcgt ttcggtgtgg ttccggtgca gggggtgcac cggcatcatg aagattccga
      961 gcctcatcga cggcctccgt ccacggaagc cgccaataat acttcagcga gtgaggcgct
     1021 cgacgaggcg ggcgttcggg cagaagctgc gataagccat acgcgatccc gattccagcg
     1081 actcgcggca ggcgccggtt gccgtgcagg ccgggcaaat gcgctcggca agcgcgatca
     1141 cgcgccttac ttctggtttg gcgagtcgct cgggattggc gccgatgcga tacagcgtgc
     1201 gcacgagcgg cgaaggtacc tgctgctccc aggcgcgcag ccaagtctca agcgcgcaat
     1261 acgcggcaac cacgaacaca atgccgccga gcgccatcac tacgaccggg tcgagcgccg
     1321 gggtcaccgc tcttcgagct tcctgaagta cgcggcgttg gggcagaagc gctcgagtcc
     1381 tgcacgcgag ctggaggcga gccattcatg gcacgcatcc acgctcgggc aaagcgcgca
     1441 gcgccgcagc gcagccgcaa gagcgcgcgg ctgttctgcc gcggcgtccg ccgggctgac
     1501 gcccgcgcgg tgcatgactt tccagatctc gagttctccg cttcgggcgg acatcctgcg
     1561 gttgagcgcg tagagcgcgg gcaacaccgc cgcgacgagg aggccgaaca gcgcgagaaa
     1621 gatgaaggtt tccatgaatg cacgctatac ccgcatcggg gccgcgcctt gacatgcgtc
     1681 aaaccctata ctggattgca atgcagcaag cgctcgtacc ggcgaagctc gctttccgcg
     1741 acggaacgcc ttactccgcc gcgtacggcg acgtgtatca cagcgctgcg ggcggcccgg
     1801 cgcaggcgcg gcacgtcttc ctcgggggca attcgcttcc cggccgctgg gcggggcgca
     1861 gccgcttcgt catcctcgag accggcttcg gcttcgggct caactttctt gctacttggc
     1921 aggcttggcg gctcgacccg cggcgctgcg cgcggctgca ctacgtttcg ctcgagaagc
     1981 acccgtttac gcaagccgat ctcaagctcg cgcacgatca ctaccccgaa ttcgcgctcg
     2041 aagcagcgca gttgcaagcg tgctggccgg tcctcgtgcc gggggcacac cgccttgagc
     2101 tcgacggcgg ccgggtggtt ctcacgcttt tcttcgccga tgtcgcgctc gcgcgcgatc
     2161 tgcgccttgc tgcggacgcg tactatctcg acggcttcgc gccggcgaag aaccccgaca
     2221 tgtggtcgcc gcaaataatg cgcgcgctct cccgcctcgc cgcgcccggc gcgacggccg
     2281 ccacctggag cgtcgcatcc gccgtgcgcg cctcgctcga ggcaaccggc tttgccgtgc
     2341 acaagcgcgt gggcttcggc gacaagcggg agatgctgac ggcaaccagc atccgcgaag
     2401 gcgatgggtt tgcgcctcta caaagacaag cggccgtgat aggcgccgga atcgcgggcg
     2461 cggcggtatg cgagcgattg tgcgcgcgcg gctgggaggt cgagctctac gagcggcgca
     2521 ccgcgccggc gcaggaagcg tccggcaatc atgcgggggc gttccatccg atcgtcacgc
     2581 cggacgacag cgtgtttgcg cgcatcacgc gcgcagcctt cctttactcc accaggcggt
     2641 ggtcgctact tgaaaacctg cgttgggatc cttgcggcgt tctccagctc gcgcgcgacg
     2701 cgaaggaaga agcctcgcag cgcgcgtcgg tcggcgcgct cgcgctgccg cccgagtacg
     2761 cgcaatacgt gacgcgcgag gaagcgtcgg cccatgccgg cgtgccggtc gcggcgccgg
     2821 gtctttggtt cccgcaggca ggatggacgc gcccgcagtc gctggtgaac gcgctcctcg
     2881 cggcctgcgg gagtcgattg aacacctcct tcgccaccga ggtgtcgcgt ctccccgaga
     2941 ctcctgtcgt cgttcttgcg aactccggcg aagcgccgaa gctgcatgcg ccgccccacc
     3001 ttcgcctgcg caaggtgcgc gggcaggtca cgcacgtgcc ggccagcgcg a
//
LOCUS       DASUNT010000408         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1109868, whole genome shotgun sequence.
ACCESSION   DASUNT010000408 DASUNT010000000
VERSION     DASUNT010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109868"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VFX94_08320"
     CDS             <1..226
                     /locus_tag="VFX94_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768224.1"
                     /translation="DRGPVGDVGERCFWRGDELVDHPSVNYGTRLFSALFSQSALNSM
                     YKPLKKKIVRIEFSSTRNCRKSLSRQQNHA"
     gene            287..733
                     /gene="mraZ"
                     /locus_tag="VFX94_08325"
     CDS             287..733
                     /gene="mraZ"
                     /locus_tag="VFX94_08325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX5768225.1"
                     /translation="MGAFHGAAVITLDAKGRVSVPTRHRTALLDGAKSLVLTAHPEGC
                     VLIYPESAWEPVRARVEGFPSFDPKASWWKRLLLGFEEHVVPDSSGRILLAPALRQHA
                     KLEREAMMVGQGHYFELWDSGVWSAKLNSALSGSATPPPGVEDFSL"
     gene            743..1693
                     /gene="rsmH"
                     /locus_tag="VFX94_08330"
     CDS             743..1693
                     /gene="rsmH"
                     /locus_tag="VFX94_08330"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX5768226.1"
                     /translation="MHEPVLAREAIEALALRPDGTYVDGTFGRGGHSRLILQRLGPAG
                     RLIALDRDPQAAEAAREIGDPRFSFFKAKFSELEMVPSGVQGMLFDLGVSSPQLDDPA
                     RGFSFRHDGPLDMRMDPEAGMSAAEWLATAEQEEIGRVIRDYGEERFAQQIAAAIVAA
                     RRSGPIARTRQLADLVARAVRTREPGQDPATRTFQAVRIHVNRELEEVSLMLPRAVAR
                     LAPQGRLALISFHSLEDRIVKRFMQSLARPEVPRGLPLRASEMPEPALRIVGRAQRAS
                     RHEITRNPRARSATLRVAERTAAPYSMPSSRIEPGARWRN"
     gene            1681..1953
                     /gene="ftsL"
                     /locus_tag="VFX94_08335"
     CDS             1681..1953
                     /gene="ftsL"
                     /locus_tag="VFX94_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEX5768227.1"
                     /translation="MAKLNLLLLAVLVACALGLVTSQHKARRAFSQLEREQALARELE
                     AEFGRLQLEASTWSLHSRVERLATRSLGLRPADPRRVRLIGRDAGR"
BASE COUNT          334 a          691 c          719 g          366 t
ORIGIN      
        1 tgatcgtggc cccgtcgggg acgtcggcga gcgctgcttc tggcgaggcg acgaacttgt
       61 tgatcatccg agcgtgaatt atggcacccg gctctttagc gccttgtttt cacaatcggc
      121 tttgaattct atgtataagc cactgaaaaa aaagatagtt cgcatcgagt tttcaagcac
      181 aagaaactgt cgtaagtcat tgtcgcgcca acaaaatcac gcttgaccgc gggctccttt
      241 gcccgtagag tggcaaaagg taggagaagg tggcagaaaa agccatatgg gagcgtttca
      301 cggggccgcg gtcatcacgc tggacgcgaa ggggcgcgtc tctgtcccca cgcgccatcg
      361 cacagcgctc ctcgacggtg ccaaatcgct tgttctcact gcgcaccccg agggctgcgt
      421 gctcatctat cccgagtcgg cctgggagcc ggtgcgtgct cgcgtggaag gctttccgag
      481 cttcgacccc aaggcgagct ggtggaagcg cctgcttctc ggcttcgagg agcacgtggt
      541 ccccgacagc tcgggccgca tccttcttgc gcccgcgctg cgccagcacg cgaagcttga
      601 gcgcgaggcg atgatggtcg gacagggcca ctacttcgag ctctgggact cgggcgtctg
      661 gtcggcaaag ctcaacagcg cgctctccgg ttcggcgacg ccgcctcccg gcgtcgagga
      721 tttctcgctg tgagcccctg tgatgcacga gccggtgctt gcgcgcgaag cgatcgaggc
      781 gcttgcgctg cggcccgacg ggacctacgt cgacggcact ttcggccgcg gcggccacag
      841 ccggctgatc ttgcagcggc tgggccccgc tggacggctg atcgccctcg atcgcgatcc
      901 gcaggcggcg gaagcggcac gcgagatcgg cgatccacgc ttcagcttct tcaaggcgaa
      961 gttctccgag ctcgagatgg tgccctcggg cgtgcagggg atgctgttcg acctgggggt
     1021 gtcgtcgccg cagctcgacg acccggcgcg cgggttctcg tttcgccatg acggcccgct
     1081 cgacatgcgc atggatccgg aggccggcat gagcgccgcc gagtggctcg cgaccgcaga
     1141 acaagaagaa atcgggaggg taatcaggga ctatggcgag gaacggtttg ctcaacagat
     1201 tgcagcggcg attgttgctg cgcgccgcag cggacccatc gcccgcacca ggcaactggc
     1261 cgatctcgtg gctcgcgcgg tccgcacacg ggagccgggc caggacccgg cgacgcgcac
     1321 ctttcaggct gtacggattc acgtcaatcg ggagcttgag gaagtgtcgc tgatgctgcc
     1381 ccgggcggtg gcgcggctcg cgccgcaggg ccgcctcgcg ctgatcagct tccactcgct
     1441 cgaggaccgc attgtgaagc gcttcatgca gtcgctggcg cgccccgaag tgccccgtgg
     1501 cttgccgctg cgcgccagcg aaatgccaga acctgcgttg agaatcgtgg gacgtgcgca
     1561 gcgcgcgtcc cgccacgaga taacaagaaa tccgcgggcg cgaagcgcca ccctccgggt
     1621 ggccgagcgc accgcggccc catattccat gccctcttca cggatcgagc ccggcgcgcg
     1681 gtggcgaaac tgaacctcct cctcctcgcg gttctcgttg cctgcgcgct cgggctcgtc
     1741 acttcgcagc acaaggcgcg ccgcgccttc tcgcagctcg agcgcgagca ggcgctcgcg
     1801 cgcgagctgg aggcggagtt cggccgcctg cagctcgagg cgagcacctg gagcctgcat
     1861 tcgcgagtcg aaaggctcgc cacgcgatcg ctcggcctgc gcccggccga cccgcggcgc
     1921 gtgcgcctca tcgggcgaga cgccgggcgg tgagcagggc ggcgcagggc ctggtgcgcc
     1981 tgccggtctg gcgtgcccgc ttggtgctga tggcgctggc gggtggcttc gcggtgctca
     2041 tggggcgcgc ggtctacctc caggccgtgc gcaccgattt cctgcaggaa aagggcgacg
     2101 ctcgctattc
//
LOCUS       DASUNT010000409         2078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1286349, whole genome shotgun sequence.
ACCESSION   DASUNT010000409 DASUNT010000000
VERSION     DASUNT010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286349"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="VFX94_08340"
     CDS             <1..1074
                     /locus_tag="VFX94_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5768228.1"
                     /translation="AEQLAPNAARWDREKTFPREALQGLARMGLYGVAIPEQWGGAGL
                     DYTSLAVACEEIAAGDCSTATIVAVNNLVAGILAGYASQKQKEAFLRPLAEGKLLGAF
                     ALTEPHVGSDAAAITTRAEKKEAHYVLNGVKQFITSGKNADVAVIFAVTDRGAGKKGI
                     SAFLVPTSTPGYVVARIEEKTGQRASDTAQIVLENCKAEHLLGEEGAGYRIALANLES
                     GRINVAAQATGVARAALEAAIAYGRERKSMGKTLIEHQAVNFRLADMATQVEAARQLY
                     LHAARLRDEGKPCLKEASMAKLFASEMAEKVCSDAIQIHGGYGYVCDFPVERLWRDVR
                     VTKIYEGANDIQRLVIGRALEEG"
     gene            1071..1646
                     /locus_tag="VFX94_08345"
     CDS             1071..1646
                     /locus_tag="VFX94_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX5768229.1"
                     /translation="MKTPIDFYFDFSSPYGYLASHKIETLGAKYGRSVTWRPMLLGAA
                     FKATGAAPLPSIPLKGDYAKRDFLRSARFHGVSFRMPDPFPVSTVSACRAFYGSPQDK
                     QVTLAKAIFRAYFVDNLNIGEAENVLKVAAGVGCKPDLNDQAVKDKTRAEVDAALAKG
                     VFGSPYIVVDGEPFWGVDRFDQIERWLREPF"
     gene            1700..>2078
                     /locus_tag="VFX94_08350"
     CDS             1700..>2078
                     /locus_tag="VFX94_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX5768230.1"
                     /translation="MRALVEDLKQKVSVVTQGGDKAARDKHTARGKMLPRERVAKLLD
                     PGSPFLEIGQLAAWGMYGGDVHSASIICGIGRVSGRECVIVANDATIKGGTYYPMTVK
                     KHLRAQEIAAQNRLPCIYLVDSGG"
BASE COUNT          395 a          663 c          697 g          323 t
ORIGIN      
        1 gccgagcagc tcgcgccgaa tgccgcgcgc tgggatcgcg aaaagacctt cccgcgggag
       61 gcgctgcaag gcctcgccag gatgggtctc tatggagtcg cgatcccgga gcaatggggc
      121 ggcgccgggc ttgactacac ctcgctcgcg gtcgcgtgcg aggaaatcgc ggcgggggat
      181 tgctccaccg cgactatcgt cgcggtgaat aacctggtcg ccggcattct cgcgggctat
      241 gcgagccaaa agcagaagga ggcgttcctc cggcccctcg cggaaggaaa gctcctcggc
      301 gccttcgcgc tcaccgagcc ccacgtcggc tcggatgccg ctgcgatcac cacgcgagcc
      361 gaaaaaaagg aggcgcacta cgtgctgaac ggcgtgaagc agttcatcac ttcgggcaag
      421 aacgccgatg tcgcagtgat ctttgccgtc accgacaggg gagcgggaaa gaaggggatc
      481 agcgcattcc tcgttccgac ctccacgccg ggatatgtcg ttgcgcgcat cgaggaaaag
      541 accgggcagc gcgcctccga caccgcgcag atcgtgctcg agaactgcaa ggcggagcac
      601 ctgctgggcg aggaaggggc cggttaccgc atcgctctcg ccaacctcga gtcgggacgg
      661 atcaacgtcg cggcacaggc gacaggcgtg gcacgcgcgg cgctggaagc ggcgatcgcg
      721 tacggccggg agcgcaagag catgggcaag acgttgatcg agcaccaggc ggtcaacttc
      781 aggctggccg acatggcgac gcaggtggag gcggcgcgcc agctttacct gcacgccgcg
      841 cggctgcgcg acgagggcaa gccctgcctt aaggaagcct ccatggccaa gcttttcgcc
      901 tcggagatgg cggagaaagt atgctcggac gcgatccaga tccacggcgg ctacggctac
      961 gtctgcgact tccccgtcga gcggctatgg cgcgatgtac gcgtgaccaa gatctacgag
     1021 ggcgccaacg acatccagcg cctcgtcatc ggaagagccc tggaggaagg atgaaaacgc
     1081 cgatcgattt ctatttcgac ttttcctccc cctacggcta tctcgcctcg cacaaaatcg
     1141 agacgctcgg cgcaaagtac ggccgcagcg tcacgtggcg cccgatgctg cttggggccg
     1201 ctttcaaagc gacgggcgcc gctccgctcc ccagcattcc gctcaagggc gactatgcga
     1261 agcgcgactt cctgcgctcg gcgcgcttcc acggcgtttc gttccgtatg ccggatccgt
     1321 ttccggtatc aaccgtgtcc gcatgccgag cgttctacgg gtcgccgcaa gacaagcaag
     1381 tgacgctcgc caaggcgata ttccgggcgt acttcgtcga caacctgaac atcggcgaag
     1441 ccgagaacgt gctaaaggtc gcggccggcg tcggctgcaa gccagatctc aatgatcagg
     1501 cggtgaagga caagacgcgc gcggaggtgg acgcggctct tgcgaagggt gtcttcggct
     1561 cgccttacat cgtggtcgac ggcgagccct tctggggcgt ggaccgcttc gaccagatcg
     1621 agcgctggct gcgcgagccg ttttgatcaa gagccagctc aagccacgct cggaggagtt
     1681 ccgcgagaac gcggcgcgca tgcgcgccct ggtggaggat ctcaagcaga aagtctcggt
     1741 cgtcacccag ggcggagaca aggccgcccg cgacaagcac acggcgcgcg gcaagatgct
     1801 gccccgcgag cgggtggcca agctgctcga cccgggctcg ccattccttg agatcgggca
     1861 gctcgcggcc tggggcatgt acggcgggga cgtgcactcg gcgtcgatca tctgcggcat
     1921 cggccgggta agcggccgcg aatgcgtgat cgtcgcgaac gacgcgacca tcaagggcgg
     1981 cacctactat cccatgacgg tgaagaagca cctgcgcgcg caggagattg ccgcccagaa
     2041 ccggctgccg tgcatctacc tcgtcgattc cggcggcg
//
LOCUS       DASUNT010000410         4821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_671714, whole genome shotgun sequence.
ACCESSION   DASUNT010000410 DASUNT010000000
VERSION     DASUNT010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671714"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..3273)
                     /gene="treS"
                     /locus_tag="VFX94_08355"
     CDS             complement(25..3273)
                     /gene="treS"
                     /locus_tag="VFX94_08355"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEX5768231.1"
                     /translation="MNERVTEVLQMPAAAEAPVEPLWYKDAVIYQLHVKAFYDSNNDG
                     IGDFKGLSSKLDYIQELGVNALWLLPFYPSPLKDDGYDVSDYHNVHPHYGTRADFQEF
                     VQEAHKRGLKVITELVVNHTSDQHPWFQAARQAPSGSAKRDYYVWSDTDTKYKGTRII
                     FTDTETSNWTWDPVAKAYFWHRFFSHQPDLNFDNPHVLEAVLRTMRFWLNIGVDGFRL
                     DAIPYLIEREGTTNENLPETHAILKQIRAVIDKEYAHRFLLAEANMWPEDVRPYFGEG
                     DECHMAFHFPLMPRMYMAIAQEDRHPVVEIMAQTPEIPDLCQWAIFVRNHDELTLEMV
                     TNRERDYMYRMYAADVQARINLGIRRRLAPLMENDPDRVKLMNSMLLSMPGSPILYYG
                     DEIGMGDNIYLGDRNGVRTPMQWSPDRNAGFSRADPQRLYLPPIMDAVYGYEAVNVEA
                     QLRDPASLLNWTRRMLATRKTSRAFGRGRLEFLRPGNRTVIAYLREFGDEAILCVANL
                     SRSAQPVELDLQRFKGRVPVEMLGRTAFPPIGERFYLFTLPAYGFYWFRLAADATPPD
                     WHTQVSPPQEQPMLVLFDSWTSFFREAVVPWRIGMAQTLRERLESQVLPAFLEAQRWY
                     AQKGEAVGRARIRDHVVWTEGAVSWLVALAEVKDSVYFLPLALAWEEEEEQMKRLGLA
                     TVARVRQQANVGVMADAMADEGFCRQVAKAIGEGRNVPTKSGALKFTPTSAYRELVPR
                     ELATLPVGRLQAQSTNTSVQLGDRLFLKCYRRVRAGVHAELEVGRFLTEVARFKNCVP
                     LAGSVEYVAPGAEPAALAMLQAYVPNQGDGWSYTAAYLERFLKAPRTDEPHGAYLTLI
                     QTLATRTAELHRALATPSGDPAFEPEPLTAQEVAAWKQRVRDEATATFARLDKAQQAA
                     LQQVLERIDTCAAPAKPTSRTRHHGDYHLGQVLLANNDFLIIDFEGEPSRPLAESRVK
                     RSPIRDVAGMLRSFAYARGAALLNAVQPAPERATADLEAWEAATRDAFLKAYADAMSG
                     SGIFDSFEDVRGLLELAEIEKILYEVRYELDNRPAWLRIPLQGLAALSPGPK"
     gene            complement(3270..>4821)
                     /locus_tag="VFX94_08360"
     CDS             complement(3270..>4821)
                     /locus_tag="VFX94_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5768232.1"
                     /translation="VARNTAQASVLRDAVTALRDTRRPPLARLHAGTDPTVRAELAAR
                     PLRDYVSPSTAYPLVVERVRALYGAWYEIFPRSEGAKHDPETDTWTSGTLRTAAQRLP
                     AIAGMGFDVVYLTPIHPIGTTARKGRNNAVVAQAGDVGSPWAIGSKDGGHKAIEKSLG
                     TLEDFDFLVREAEKRKIQIALDIAFQCSPDHPYVKEHPEWFRARPDGTIQYAENPPKK
                     YQDIYPFDFETPAWQSLWQELKSVFEFWIGHGVKVFRVDNPHTKTFAFWEWAISDLKR
                     KHPDLIFLSEAFTRPRVMHKLAKLGFTQSYTYFTWRNTKAELMEYFTELTQHASREYF
                     RPNVWPNTPDILHATLQTGGRPAFIARAVLAATAAANYGIYGPAFELQERTPREPGSE
                     EYRHSEKYEVKRWDLERSDSLAPLIARLNAIRKANRALQSDWSLRFHATDNDQLLAYS
                     KKDGDNEILVAVNLDPNDRQSGWVDFRPCEVDDLLSGGHYVWRANRNYIELNPHTLPA
                     HVFLVRRT"
BASE COUNT          725 a         1557 c         1665 g          874 t
ORIGIN      
        1 tccatgtcta ctccctccgg ttcgtcactt cggccctggg ctcagcgcgg cgagcccctg
       61 cagcgggatg cgcagccacg ccgggcggtt gtcgagctcg tagcgcacct cgtagaggat
      121 cttctcgatc tcggcgagct ccagcagccc gcgcacgtct tcgaatgaat cgaatatccc
      181 ggagccgctc atggcgtcgg cgtaggcctt gaggaacgcg tcgcgcgtgg cggcttccca
      241 cgcttcgaga tcggccgtgg cgcgctcggg ggcgggctgc acggcgttca ggagcgctgc
      301 tcctctcgcg tacgcgaagg aacgcagcat gcccgccacg tcgcggattg gcgagcgctt
      361 gacccggctc tcggcgagcg ggcgcgaagg ctcgccctcg aagtcgatga tgaggaagtc
      421 gttgttggcg aggagcacct gcccgaggtg atagtcgccg tggtgccgcg tcctggacgt
      481 cggcttcgcg ggcgcagcgc aggtatcgat ccgctcgagg acctgctgca gcgcggcctg
      541 ctgcgccttg tccagcctgg cgaaggtggc tgtcgcttcg tcacgcacgc gctgcttcca
      601 cgccgccact tcctgcgccg tgagcggctc gggctcgaac gccggatcgc cgctcggcgt
      661 cgccagcgcg cggtgcagct ccgcggtgcg cgtagcgagc gtctgaatca gcgtgagata
      721 cgcgccgtgc ggctcatcgg tgcgcggcgc tttgaggaag cgctcgaggt acgcggccgt
      781 gtagctccag ccgtcgccct ggttgggcac gtaggcctgc agcatggcca gcgcagccgg
      841 ctcggcgccc ggcgcgacgt attccaccga gcccgcgagc ggcacgcaat tcttgaagcg
      901 cgcgacttcg gtgaggaagc gcccgacctc gagctcggca tggacgcccg cgcgcacgcg
      961 gcggtagcac ttgaggaaga gtcggtcgcc gagctgcacc gacgtattgg tgctctgtgc
     1021 ctgcaggcgg ccgacgggca acgtggcgag ctcgcgcggg acgagctcgc ggtaggcgct
     1081 ggtcggcgtg aatttcagtg cgcccgactt ggtcggcacg ttgcgcccct cgccgatcgc
     1141 cttggcgacc tgccggcaga atccctcgtc ggccatcgcg tcggccatca cgccgacgtt
     1201 cgcctgctgc cgcacccgcg cgaccgtggc gagcccaagc cgcttcatct gctcctcttc
     1261 ctcctcccag gcgagcgcga gcggaaggaa gtagaccgag tccttcacct cggccagggc
     1321 gacgagccag ctcaccgcgc cctccgtcca caccacgtgg tcgcggatcc tcgcgcgccc
     1381 caccgcttcg cccttctgcg cataccagcg ctgcgcctcg aggaacgcgg gcagcacctg
     1441 gctttcgagc cgctcgcgca gcgtctgcgc catgccgatg cgccagggaa ccaccgcttc
     1501 gcggaaaaag ctggtccagc tgtcgaagag gacgagcatc ggttgctcct gtggcgggga
     1561 cacctgcgtg tgccagtcag gcggcgtggc gtcggcggcg aggcgaaacc agtagaagcc
     1621 gtacgcgggc aaagtgaaga gatagaagcg ctcgccgatg ggcgggaacg ccgtacggcc
     1681 gagcatctcg accggcacgc gccccttgaa gcgctgcagg tcgagctcga ccggctgcgc
     1741 gctgcgcgag aggttcgcga cacacaggat cgcttcgtcg ccgaactcgc gcaggtacgc
     1801 gatcactgtc ctgttgccgg ggcgaaggaa ctccagccgg ccgcggccga aggcgcggct
     1861 cgtcttgcgc gtcgcgagca tgcgcctggt ccagttgagc agcgaggccg ggtcgcgcaa
     1921 ctgcgcctcc acgttcaccg cttcgtagcc atagacggcg tccatgatcg gcggcaggta
     1981 cagccgctgc ggatcggcgc gggagaagcc ggcattgcgg tccgggctcc actgcattgg
     2041 cgtcctcacg ccgttacgat cgccgaggta gatgttgtcg cccatgccga tctcgtcgcc
     2101 gtagtacagg atcggcgagc ccggcatgga gagcagcatg ctgttcatca gcttgacgcg
     2161 gtcggggtcg ttctccatga gcggcgcgag gcgccggcga atgccgaggt tgatgcgcgc
     2221 ctggacgtcc gcggcgtaca tgcggtacat gtagtcgcgc tcgcggttgg tcaccatctc
     2281 gagcgtgagc tcgtcgtggt tgcgcacaaa aatcgcccac tggcacaggt ccgggatctc
     2341 gggcgtctgc gccatgatct cgaccaccgg gtggcggtct tcctgcgcga tcgccatgta
     2401 catgcgcggc atcagcggaa agtggaacgc catgtggcat tcgtcgcctt cgccgaaata
     2461 cggccgcacg tcctcgggcc acatgttggc ctcggcgagc aaaaagcggt gcgcgtactc
     2521 cttgtcgatc accgcgcgga tctgcttcag gatcgcgtgc gtttcgggca ggttctcgtt
     2581 cgtcgtgcct tcgcgctcga tgaggtacgg gatcgcgtcg aggcgaaagc cgtccactcc
     2641 tatgttgagc cagaagcgca tcgtgcgcag caccgcctcg agcacgtgcg ggttgtcgaa
     2701 gttgaggtcg ggctgatggc tgaagaagcg gtgccagaag tacgcttttg ccaccgggtc
     2761 ccacgtccag ttcgatgtct cggtgtcggt gaagatgatg cgcgtgccct tgtacttcgt
     2821 atcggtgtcg ctccatacgt agtagtcgcg cttcgccgag ccggacggcg cctggcgggc
     2881 cgcctggaac cagggatgct ggtcggaggt gtggttcacc acgagctcgg tgatcacctt
     2941 gagcccgcgc ttgtgcgcct cctgcacgaa ctcctggaaa tccgcgcgcg tgccgtagtg
     3001 cgggtgcacg ttgtggtaat cggacacgtc gtagccgtcg tccttgagcg gcgagggata
     3061 gaacggcagc agccacagcg cgttgacgcc aagctcctgg atgtagtcga gcttggagct
     3121 cagacccttg aagtcgccga tgccgtcgtt gttcgagtcg tagaacgcct tgacgtgcag
     3181 ctggtagatg accgcgtcct tgtaccagag gggctcgacc ggagcttcgg ccgccgccgg
     3241 catctgcagg acttcggtga cgcgctcgtt catgttctgc gcaccaggaa tacgtgggcc
     3301 ggaagcgtgt gcggattgag ctcgatgtag ttgcggttgg cgcgccacac gtagtgcccg
     3361 ccggaaagca gatcgtccac ctcgcagggc cggaaatcga cccatcccga ctgcctgtca
     3421 ttcggatcca ggttcaccgc aaccaggatc tcgttgtcgc cgtccttctt gctgtaggcg
     3481 agcagctggt cgttatccgt cgcgtggaag cgcaggctcc agtccgactg cagcgcacgg
     3541 ttcgccttgc ggatggcgtt cagcctcgcg atgagcgggg cgaggctgtc ggagcgctcg
     3601 aggtcccagc gcttcacctc gtatttctcg gagtgccggt attcctcgct cccgggctcg
     3661 cgcggcgtgc gctcctggag ctcgaacgcc ggcccgtaga tcccgtagtt ggccgccgcg
     3721 gtggctgcga gcaccgcgcg cgcgatgaag gccggccggc cgcccgtctg cagcgttgcg
     3781 tgcaggatat cgggtgtatt cggccacacg ttcggccgga aatactctct ggaggcatgt
     3841 tgcgtgagct cggtgaaata ctccatcaac tcggccttcg tgttgcgcca ggtgaagtac
     3901 gtgtaagact gggtaaagcc aagcttcgcg agcttgtgca tcacgcgcgg ccgggtgaaa
     3961 gcctcggaga ggaaaatgag atccgggtgc tttctcttca ggtcggagat cgcccattcc
     4021 cagaacgcga acgttttggt gtgcgggttg tcgactcgaa agaccttcac tccgtggccg
     4081 atccagaact cgaacaccga cttcagctcc tgccacagcg actgccacgc cggcgtttcg
     4141 aaatcgaagg gatagatgtc ctggtacttc ttcggcgggt tctccgcgta ctggatcgtg
     4201 ccgtcgggcc gcgcgcgaaa ccactcgggg tgctctttca cgtacggatg gtcgggcgag
     4261 cactggaatg cgatgtcgag cgcgatctgg atcttccgct tttcggcttc acgaaccagg
     4321 aagtcgaagt cctccagcgt gccaagcgat ttctctatgg ccttgtgacc gccgtccttc
     4381 gagccgatcg cccacgggct gccgacgtcg ccggcctgcg cgacgacggc gttgttcctg
     4441 cccttgcgcg ccgtcgtgcc gatcgggtgg atcggggtca ggtagacgac gtcgaagccc
     4501 atgccggcga tggcgggcag ccgctgggcg gcggtgcgca gggtgcccga cgtccaggtg
     4561 tcggtctcgg ggtcgtgctt cgccccctcc gagcggggga agatctcgta ccacgcgccg
     4621 tagagcgcgc gcacgcgctc gacgaccagc gggtatgccg tggacgggct cacgtagtcg
     4681 cgcaggggcc gcgcggcgag ctcggcccgg accgtcgggt cggtgccggc gtggaggcgt
     4741 gcgagcggcg ggcggcgggt gtcgcgcagc gcggtcacgg cgtcccggag cacggacgcc
     4801 tgggccgtgt tgcgggccac c
//
LOCUS       DASUNT010000411         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_847387, whole genome shotgun sequence.
ACCESSION   DASUNT010000411 DASUNT010000000
VERSION     DASUNT010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847387"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..761)
                     /gene="tpiA"
                     /locus_tag="VFX94_08365"
     CDS             complement(45..761)
                     /gene="tpiA"
                     /locus_tag="VFX94_08365"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX5768233.1"
                     /translation="MARARLVAGNWKMHGSRAWIAALLDALVEENPSSCAVCVPFPYL
                     AQVAERLRGSPIAWGAQSVSEHAAGAYTGEVSASMLAEFGCRYVIVGHSERRQLYGET
                     DGQVAAKFAAARSAGMIPILCVGETLAEREAGGTQAVVERQLLAVLERNAFQNSVLAY
                     EPVWAIGTGRNATPEQVQAVHEILRQRVGAETPILYGGSVKPQNAAAIFAMPDVDGGL
                     IGGASLVARDFLDIVKAAAK"
     gene            complement(773..1213)
                     /locus_tag="VFX94_08370"
     CDS             complement(773..1213)
                     /locus_tag="VFX94_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768234.1"
                     /translation="MRKDALAIRSGQGTGELLRAARQIDREFIARLERFPLVRLRIRY
                     EDIEPIRRERIERLLGATRALVAAPWRGIRAAARARYSADQLEALVRKHLRLYAAEVD
                     SLGACVRPRLLVAPFRSRLRALMDERAGALAREVAHLLYDRAMR"
     gene            complement(1345..>2146)
                     /locus_tag="VFX94_08375"
     CDS             complement(1345..>2146)
                     /locus_tag="VFX94_08375"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5768235.1"
                     /translation="GPQFTICSNYKEEGTMPPPPIAMREQLREERFNGVWTQELNRVF
                     ADVAPYYDQANVVASLGLWDFLLRGFMDTMQLRAGERVLDVCAGTNAVGIALLKREPG
                     LEVHAMDRSAEMQEVGRSRAQALGMRIHSVIGDVHKLPFPDNHFDIVTLQWATRHLRV
                     KQVFGEIRRVLKPGGRFYHCDMLRPANPIVETLYYTYLRLCLNFTALVFGSGASAHGL
                     KRYFINALEMFYSADELSELLRGLRFERVAARTVLAGMIGYHRAAKPA"
BASE COUNT          350 a          753 c          711 g          332 t
ORIGIN      
        1 gaccagcacg tgcaccacga tcaggatgtt ggtccagaag ctcgtcattt cgccgccgcc
       61 ttcacgatat ccagaaagtc cctggcgacg agcgatgcgc cgccgatcag gccgccgtcg
      121 acgtcgggca tcgcaaaaat cgccgccgca ttctgcggct tcacgcttcc cccatacaag
      181 atgggcgttt cggcgcccac tcgctgcctg aggatctcat gcaccgcctg cacctgctcg
      241 ggcgtcgcgt ttcgcccggt gccgattgcc cacaccggct cgtaggccag aaccgaattc
      301 tggaaagcat tccgttccag cactgcgagc aactgccgct ccaccactgc ttgcgtgccg
      361 ccggcctcgc gctcggccag cgtttcgccc acgcacagga tcgggatcat gcccgccgag
      421 cgtgccgcgg caaacttggc cgccacctgg ccgtcggtct caccgtagag ttgccgccgc
      481 tcggagtgcc cgacgatcac gtagcggcag ccgaattccg ccagcatcga cgccgacacc
      541 tctccggtat aggcgcccgc ggcgtgctcc gagacgctct gcgcccccca cgcgatcggg
      601 ctgccgcgca gccgttctgc cacctgtgcc agatacggaa acggcacgca taccgcacac
      661 gatgaggggt tttcctcgac aagcgcatcg agcagcgccg cgatccaggc ccggctgccg
      721 tgcattttcc agttgccggc gaccagccga gcgcgcgcca tgcaggagaa ttttagcgca
      781 ttgcacggtc ataaagcagg tgcgccacct cgcgcgcgag cgcgccggcc cgctcgtcca
      841 tgagcgcgcg cagtcggctg cgaaacggcg ccacgagaag ccgcgggcgc acgcaggcgc
      901 cgagcgagtc cacctcggct gcgtaaaggc gaagatgctt gcgcaccagt gcttcaagct
      961 ggtcagcgct gtagcgcgcg cgcgccgcag cccggatgcc gcgccatggc gctgcgacga
     1021 gcgcgcgggt ggcaccgagc aggcgctcga tgcgctcgcg gcgaatcggc tcgatgtcct
     1081 cgtagcggat acgcaggcga acgagcggga agcgctccag gcgcgcgatg aactcgcggt
     1141 cgatctggcg cgcggcgcgc agcagctcgc ccgtgccctg cccggaccgg atggcgagcg
     1201 cgtccttgcg cacttctttc tcgacattgg cggcgaggag cgcctcgagc gggccgagcg
     1261 ggagccgggc cgcggcgatg cagcggccgc tataggcgcg cagcagccgg ccattcagcg
     1321 acgcgagggc gcgcgcgcca tccttcaggc gggcttggcg gcgcggtggt agccgatcat
     1381 cccggcgagc acggtgcgcg ccgcgacgcg ctcgaagcga agcccgcgca gcagctcgga
     1441 gagctcgtcg gccgaataga acatctccag cgcgttgatg aagtagcgtt tgagcccgtg
     1501 cgcggaggcc ccgctgccga agacgagcgc ggtgaaattc aggcagagac gcaggtacgt
     1561 gtaatagagc gtctccacga tcgggttcgc gggccgcagc atgtcgcagt ggtagaagcg
     1621 cccgccgggc ttgagcaccc gccggatctc gccgaatacc tgcttcacgc gcaggtgacg
     1681 cgtcgcccac tgcagcgtca cgatgtcgaa gtggttatcc gggaagggga gcttgtgcac
     1741 gtcgccaatc acgctgtgga tgcgcatgcc gagcgcttgg gcgcggctgc ggcctacttc
     1801 ctgcatctcc gcgcttcggt ccatggcgtg cacctcgagt ccgggctcgc gcttgagcag
     1861 agcgatgccc accgcgttcg tgccggcgca cacgtcgagc acgcgctcgc ccgcgcgcag
     1921 ctgcatcgtg tccatgaagc cgcgcagcag gaagtcccac agtccgaggc tcgcgacgac
     1981 gttcgcctgg tcgtagtacg gcgccacgtc ggcgaacacc cggttcagct cctgcgtcca
     2041 gacaccgttg aagcgctcct cgcggagctg ctcgcgcatg gcgatgggcg gcggcggcat
     2101 ggtcccctcc tctttatagt ttgagcagat agtaaactgc ggcccc
//
LOCUS       DASUNT010000412         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_672394, whole genome shotgun sequence.
ACCESSION   DASUNT010000412 DASUNT010000000
VERSION     DASUNT010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672394"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="VFX94_08380"
     CDS             <1..1090
                     /locus_tag="VFX94_08380"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5768236.1"
                     /translation="TEREMKVFFYVQHLLGIGHLRRAAAIAHALRQSGIEVTLASGGA
                     PVEGIAVLQLPPAASDASFKLLDERGEPVDEAGKERRRARLLEAYRASKPDALVVELF
                     PFGRRQMRFELLPLLEEASARLAVCSVRDILQPRPEREEEALEQFERYFDRLLVHGDP
                     RVAPFERSFSRAARLAQRLHYTGYVVEEAPAAADTGAGEVLVSAGGGAVGRRLVETAI
                     RARPLTGQRGRTWRILAGVNAKDVETLQSDGVIVERSRADFTRRLRNCVLSVSQAGYN
                     TVVETLQAGARSVLVPFAGGGELEQTLRARLFAERGLVKVVEEAALSAQTLAAAIDRA
                     AAAPRPPPGAVDLGGAQRSALLIREWLA"
     gene            1087..1821
                     /locus_tag="VFX94_08385"
     CDS             1087..1821
                     /locus_tag="VFX94_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768237.1"
                     /translation="MSWMRLDAELARRCDAGRPARFWWRDDDAGAVQPEVKRLLRLAS
                     SSGVPVSLAVVPEAANAELFGLLHDGVTVLQHGTDHRNRAAAGEKKTEYPAAEPIEAA
                     LARASDGLGRLRAFAGARFCPVLAPPWNRVRSDLLDKLPAIGIRGLSMYGPGAKAEAA
                     PGLRQVNTHVDIVAWREGKRFIGEEHAIGATLKILEREEPVGLLTHHAVHDCATWSFL
                     ERFLKTKNIRWLSAAEVFSYTAPAHV"
     gene            1814..>2146
                     /locus_tag="VFX94_08390"
     CDS             1814..>2146
                     /locus_tag="VFX94_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5768238.1"
                     /translation="MSDLLNVRDLKVYLDVDAGTVKAVDGITFRVPAGRTVALVGESG
                     SGKSVVAQSIMSILPRIARIESGEIIFRDPERRPGTEIDISRLDPESREMQDIRGGRI
                     SIIFQEPMT"
BASE COUNT          335 a          700 c          780 g          331 t
ORIGIN      
        1 gactgaacgt gaaatgaagg tatttttcta cgttcagcat ctgctcggca tcgggcacct
       61 caggcgcgcg gcggcgatcg cgcacgcgct caggcagtcc ggaatcgaag ttaccctcgc
      121 gagcggcggc gcgccggtgg agggcatagc ggtactgcag ctcccgcccg cggcgagcga
      181 tgcttctttc aagctgctcg atgagcgcgg cgagccggtc gacgaggcgg ggaaggagcg
      241 ccgccgcgcc cgcctgctgg aggcataccg cgcaagcaag cccgacgccc tcgtggtgga
      301 gctctttccg ttcggccgcc ggcagatgcg cttcgagctg ctgccgctcc tcgaggaggc
      361 gagcgcgcgg ctcgccgtgt gctcggtgcg cgacatcctg cagcccaggc ccgagcgcga
      421 agaggaggcg ctcgagcagt tcgagcgcta cttcgaccgc ttgctggtgc acggcgaccc
      481 gcgcgtcgcg cccttcgagc gcagcttctc gcgcgcggcg cgcctggcgc aaaggctcca
      541 ctacaccggc tacgtggtcg aggaggcgcc ggcggcggcg gacaccggag ccggagaagt
      601 actcgtgtcg gcgggcgggg gcgcggtggg gcgaaggctt gtcgaaaccg cgatccgggc
      661 gcgtccgctt acgggccagc gcggccgtac atggcgcatc ctggccggcg tcaacgcgaa
      721 ggacgtagag accctgcaaa gcgacggggt aatagtggag cgctcgcgag cggacttcac
      781 ccggcgcctt cgaaactgcg tactctccgt aagccaggcc ggctacaaca cggtggtaga
      841 aaccctgcag gccggtgcgc gctcggtgct cgtgcccttc gccggcggcg gagagctcga
      901 gcagacgctg cgcgccaggc tgtttgcgga gagggggctc gtgaaggtgg tggaggaggc
      961 agcgctttcc gcgcagacgt tggcggcggc aatcgaccgc gccgcggccg ccccacgccc
     1021 cccgccgggc gcggtggacc ttggcggcgc gcagcgcagt gcgctgctca tccgcgagtg
     1081 gctggcatga gctggatgcg gctcgacgcc gagctcgccc gccggtgcga tgccggccgg
     1141 ccggcgcggt tctggtggcg cgacgacgat gcgggagcgg tgcagccgga ggtaaagcgc
     1201 ctgctgcgcc tcgcttcctc ttccggagtc ccggtctccc tggccgtcgt tcccgaggcg
     1261 gccaatgccg aactcttcgg tctcctgcat gacggcgtta ccgtgttgca gcacggaacg
     1321 gaccatcgca accgggcggc ggccggcgag aagaagaccg aatatccggc ggccgagccg
     1381 atcgaggcgg cccttgccag ggcatcggac ggcctcggcc ggctgcgtgc gttcgccggc
     1441 gcaaggtttt gcccggttct cgcaccgccg tggaaccgcg tacgaagcga cttgctggac
     1501 aagctgccgg cgatcggcat ccggggcttg agcatgtacg ggccgggcgc gaaagcggaa
     1561 gctgcgccgg ggctgcggca ggtaaacact cacgtggaca tcgtggcgtg gcgcgaaggc
     1621 aaacgcttca tcggcgaaga gcacgcgatc ggcgctaccc tgaagattct cgagcgcgaa
     1681 gagcccgtgg gcttgcttac gcatcacgcg gtgcacgact gcgcgacctg gagttttctg
     1741 gaacggttcc tcaagacgaa gaacatacgc tggctctctg ccgcagaagt gttctcgtat
     1801 actgcgccgg ctcatgtctg accttctgaa cgtccgcgac ttgaaggtct atctggacgt
     1861 ggacgccggg acggtaaagg cggtggacgg catcacgttc cgcgtcccgg ccggccgcac
     1921 cgttgcgctc gtgggcgagt ccggttcggg caagtccgtg gtcgcgcagt cgatcatgag
     1981 catcctgccg cgcatcgcgc gcatcgagtc cggggagatc atctttcgcg acccggagcg
     2041 ccggccgggg accgagatcg acatctcgcg gctcgacccg gagagccgcg agatgcagga
     2101 catccgcggc ggacgcatct cgatcatctt ccaggagccg atgacg
//
LOCUS       DASUNT010000413         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_498120, whole genome shotgun sequence.
ACCESSION   DASUNT010000413 DASUNT010000000
VERSION     DASUNT010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498120"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFX94_08395"
     CDS             complement(<1..252)
                     /locus_tag="VFX94_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5768239.1"
                     /translation="MDAEQQDALTKIRRFYDSVYYGRIAERRPVSSHLRRLARRIGIT
                     QGMRLLDVGCGTGEWLRAARERGAQPSGIDLSHKATDACK"
     gene            complement(254..1132)
                     /locus_tag="VFX94_08400"
     CDS             complement(254..1132)
                     /locus_tag="VFX94_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5768240.1"
                     /translation="MSKTLSLSVILPAKNEAHNLQALLPRVQAMLTGVNRDAEIIVID
                     DGSTDGTRQICEAHGVRVISHPYSSGNGAAVKTGARAARGEVLAFMDADGQHDPELIP
                     RLLDKLDEGYDMVVGARTAQAHAGAHRFAANAFYNWFASWMIGRPVPDLTSGLRVARS
                     ERFRRFLYLLPNGFSYPTTSTMSFFRSGYPVAYVPVPVQRRVGRSHIRLLRDGLRFLL
                     IIFKIGTLYSPLKLFVPISAAFFMTGLGYYLYTYVTTNRFTNMSALLFVTSILVFVVG
                     LLSEQITLLNFKDSDK"
     gene            complement(1129..>2019)
                     /locus_tag="VFX94_08405"
     CDS             complement(1129..>2019)
                     /locus_tag="VFX94_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768241.1"
                     /translation="IPLELYFEHRNYLPLLGPVLALVWGAKHLWDAASTPGMRRVYAS
                     LGIGVGVALAGVTWVEARTWADPVGQVAVWANERTGSQRAQYALGVQYLYAGRYADAS
                     DAWARAQSMAPQEGYFDLARLLLACLAEEVPVPEPSRVALRLAQAPLRSVTIELLDNL
                     ALQFERGKCRRVSVEGALAVAEGLAANPRAAGGQHEWAALYVKGRLHAVRGELDPAVR
                     ALEGADEVLPNLGVLNLQVSWLVSAELYKEALEFIEKGRRDPRWRPWQRVLYPSFFDS
                     WERQVREAARGKGVLIRAER"
BASE COUNT          365 a          677 c          653 g          324 t
ORIGIN      
        1 cttgcacgcg tcggtcgcct tgtgcgatag gtcgatgccg ctcggctgcg ccccgcgctc
       61 gcgcgccgcg cgcagccatt caccggtgcc gcagccgaca tcgagcaggc gcatgccttg
      121 ggtaataccg atgcgccgcg cgaggcgtcg gaggtgactc gaaaccggcc ggcgctcggc
      181 gatccggccg taatagaccg aatcgtagaa gcggcggatt ttcgttagcg cgtcctgctg
      241 ctcggcgtcc actctacttg tcgctgtcct tgaagttgag cagggtgatc tgctcggaca
      301 gcaagccgac gacgaaaacc agaatcgagg tgacgaaaag cagcgcgctc atgttggtaa
      361 agcggttcgt ggtcacgtat gtgtacaggt agtagccgag gccagtcatg aagaacgccg
      421 cgctgatcgg cacgaacagc ttgagcggcg agtacagcgt tccgatcttg aatatgatga
      481 gcaggaagcg aaggccgtcg cgtagcagcc ggatgtggct tctccccacc cggcgctgca
      541 cgggcaccgg cacgtacgcg accggatagc cgctgcggaa aaagctcatg gtgctggtgg
      601 ttgggtagga gaagccgttc ggcagcaggt acaggaagcg gcgaaagcgc tcgctcctcg
      661 ccacgcgcag cccggacgtg agatccggca ccggccgccc gatcatccag ctcgcgaacc
      721 agttgtaaaa cgcgttggcg gcaaagcggt gggcgcccgc atgcgcttgc gcagtgcggg
      781 cgcctacgac catgtcgtag ccctcatcga gcttgtcgag caggcgcgga atcagctcgg
      841 ggtcgtgctg gccgtcggcg tccatgaagg cgagcacttc gccgcgcgcg gcgcgcgcgc
      901 cagtcttcac ggccgcgccg ttgccgctgg aatagggatg cgagatcacg cgaacgccat
      961 gcgcctcgca tatctggcgc gtgccgtcgg tcgagccgtc gtcgatcacg attatttcgg
     1021 catcgcggtt cacgcccgtg agcatcgcct gcacacgggg aagcagcgcc tgaaggttat
     1081 gagcctcgtt cttcgccggc aggatgaccg acaggctcaa ggtcttgctc atcgctcggc
     1141 ccggatcaga acccctttgc cccgcgccgc ttcgcgtacc tgtcgctccc acgaatcgaa
     1201 aaaactcggg tacagcacgc gctgccacgg ccgccagcgc ggatcgcgcc ggcccttttc
     1261 gatgaattcg agcgcctctt tatagagctc ggcagagaca agccagctga cctgcagatt
     1321 gagcacgccc agattcggca gcacctcgtc agcgccctcc agggcgcgaa ccgccggatc
     1381 gagctcgccg cgcacggcgt gcaagcggcc cttcacatac agggccgccc attcatgctg
     1441 gccgccggcc gctctgggat tcgcggccag cccctcggcc acggcgagcg caccttcgac
     1501 gctgacccgg cgacacttgc cgcgttcgaa ctgaagcgcc aggttatcga gcagctcgat
     1561 cgtgaccgaa cgcagcggcg cttgcgcgag gcgcagcgcg actcggcttg gctcgggcac
     1621 cggcacctct tccgcaaggc aggcgagtaa caggcgcgcg agatcaaagt aaccttcctg
     1681 cggcgccatc gactgcgcgc gcgcccacgc atcgctcgca tcggcgtagc ggcccgcgta
     1741 gagatattgg acgccgagcg cgtattgagc gcgctgcgaa ccggttcgtt cgttcgccca
     1801 aaccgccact tggcccacag ggtcggccca tgtcctcgcc tcgacccacg tcacgcccgc
     1861 caacgccacg ccgacgccga tgccgagcga agcgtacacg cgtcgcatgc ccggcgtact
     1921 ggcggcgtcc cagaggtgct tcgcgcccca caccaaagcg aggaccgggc cgagcagcgg
     1981 caggtagttg cgatgctcga aataaagctc gagcggaat
//
LOCUS       DASUNT010000414         2427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_760393, whole genome shotgun sequence.
ACCESSION   DASUNT010000414 DASUNT010000000
VERSION     DASUNT010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760393"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VFX94_08410"
     CDS             <1..386
                     /locus_tag="VFX94_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5768242.1"
                     /translation="GNDSSVAQLLERVRLAHLAEQPVSSLSHGQQRQLEIGMALAGAP
                     RLILFDEPAAGLSPAERAELVALLTSLPPHIGFVLIEHDLDIALRVVGRVTVMHNGRI
                     LKEGTPQEIEEDPEVQAIYMGGAVH"
     gene            494..787
                     /locus_tag="VFX94_08415"
                     /pseudo
     CDS             494..787
                     /locus_tag="VFX94_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1295..>2427
                     /locus_tag="VFX94_08420"
     CDS             1295..>2427
                     /locus_tag="VFX94_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HEX5768243.1"
                     /translation="MAQTAGRSAYIAGTFDTKGRELFYLRSCLEKLGLRTVTVDLATS
                     GKPSPAMIHPREVARHHPKGERAIFTGDRGSAVAEMAVAFEHFVAGRRDLGGLISAGG
                     SGGTALATRAMRRLPIGIPKVMVSTVASGDVKPYVGPSDICMMYSVTDVSGINRISEK
                     VLANAAHALAGMIAYSGSRRDASESKPALGLTMFGLTTPCVQAVCKQLEERYDCIVFH
                     ATGTGGQSMEKLVDSGLLAGVLDVTTTEIADELVGGVLTAGPARLDVFSRKGIPYVAS
                     CGALDMVNFWAMDTVPAQFRSRLLHRHNPNVTLMRTTAEEMARIGRFIAEKLNRMQGP
                     VRFLIPEGGVSGIDAPGKPFWDPAADRALFEAISSSFRSGSNR"
BASE COUNT          427 a          805 c          800 g          395 t
ORIGIN      
        1 cggggaacga ctcgagtgtc gcccagctgc tcgagcgcgt gcgcctggcg cacctggccg
       61 agcagccggt gtcctcgctc tcgcacggcc agcagcgcca gctcgagatc ggcatggcgc
      121 tcgccggcgc gccgcgcctc atcctcttcg acgagccggc ggcgggtctc tcgccggcgg
      181 aacgcgcgga gctcgtcgcg ctcctcacct cgctgccgcc gcatatcggc ttcgtcctga
      241 tcgagcacga cctcgacatc gcgctgcgcg tggtcgggcg cgtcacggtc atgcacaacg
      301 gccgcatcct caaggaaggc acgccgcagg agatcgagga agaccccgag gtccaggcga
      361 tctatatggg aggagccgtt cattgatcct cgaggtgcgc aacctcgacg tgcactacgg
      421 ccgcgcccac gcgctgcaag gggtttcgct gtcgctcgac aagggaatcg tcgcggtggt
      481 cggccgcaat ggcatgggca agacgacgct ttgcaacgcg atcaccggcc tcgtgcccgc
      541 cgccggcagc gtcaagttca acggcgagga gatcctcggc cttccggcgc acgtgatcac
      601 cgagaaaggc atcggctacg tgccgcaggg gagacgcgtc tggccctcgc tttccgtgga
      661 cgagcatctc cgccttgcgg cccgaaaggg cgcgtggacc gtcgagcgga tctacaagac
      721 gtttcctcgt cttgcggaaa gaaagggcaa cggcggcgcg gagctctcag gcggcgagca
      781 gcagatgctt gcgatctctc gcgcgctgct cttcaacccg aagctcctcg tcatggacga
      841 gccgaccgag gggctcgcgc ccgtcatcgt ccagcacgtc tcgggcatgc tgaaggcgct
      901 cgccgccgag agtgagatct cgatgcttct catcgagcag aacctcggcg tggcgatcga
      961 cgtcgccgac accatcgacg tcatggtcaa cgggcggatc gcgcgctcca tgcccgcttc
     1021 tgagctcgcc accgaccgcg agctccagca gcggctcctt ggcgtcaaga tggcggaaga
     1081 agagatcccg gtcgttgagc aaccccagtc gccgacccag gaagtgcgcg tgcttaccgt
     1141 gaagcgcgcg agcgatgagc tcgttcccga gccgcatgac ggcgagcgca cgctcggcgg
     1201 gtacacccgc tggggtgtat caaggacaag cccccagatt gaagagcaaa gagacgaaga
     1261 acaaaggacc gaagcccgga tcgtggaatt cccggtggcg cagacggcgg gtcgcagcgc
     1321 gtatatcgcc ggtacgttcg acaccaaggg ccgggagctc ttctacttgc gcagctgcct
     1381 ggaaaagctc ggcttgcgaa cggtcacagt ggatctcgcc acttccggaa agccctcgcc
     1441 ggccatgatc cacccgcgcg aagtcgcccg gcaccatccg aagggcgagc gcgcgatatt
     1501 taccggcgat cgcggttcgg cggtggccga gatggcggtc gctttcgagc acttcgtcgc
     1561 aggcagacga gacctcggcg gcctcatctc cgccggaggc tcgggcggca ccgcgctcgc
     1621 cacgcgcgcg atgcgccgtc tgccgatcgg cattcccaag gtcatggtct cgacggtggc
     1681 atcgggcgac gtcaagcctt atgtcggacc ctccgacatc tgcatgatgt actcggtgac
     1741 cgacgtgtcg ggcatcaacc gcatctcgga aaaggtgctg gcaaacgccg cgcacgccct
     1801 tgctggaatg attgcttact cgggatccag gcgggacgcc tccgaatcga agcctgcttt
     1861 gggcctcacg atgttcgggc tgaccacgcc gtgcgtgcag gcggtgtgca agcagctcga
     1921 agagcgctac gactgcatcg tgttccacgc gaccggcacc ggcggccaat cgatggagaa
     1981 gctcgtcgac tcgggcctgc tcgccggcgt actcgacgtc accaccaccg aaattgccga
     2041 cgagctggtg ggcggcgtgc tgactgcagg gccggcacgc ctggatgtgt tttccagaaa
     2101 aggcattccg tacgtggcat cgtgcggcgc gctcgacatg gtgaacttct gggccatgga
     2161 cacggtgccg gcgcagttcc gcagccggct tctgcaccgg cacaatccga acgtcacgct
     2221 gatgcgcacc acggccgaag agatggcgcg catcgggcgc ttcatcgccg agaagctcaa
     2281 ccgcatgcag ggccccgtgc gcttcctgat tcccgagggc ggcgtgtcgg gcatcgacgc
     2341 gccgggcaag cctttctggg atcccgcggc ggacagagcg ctttttgaag cgatcagctc
     2401 gagcttccga tcgggttcga atcgaaa
//
LOCUS       DASUNT010000415         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_147935, whole genome shotgun sequence.
ACCESSION   DASUNT010000415 DASUNT010000000
VERSION     DASUNT010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147935"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="VFX94_08425"
     CDS             <1..166
                     /locus_tag="VFX94_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX5768244.1"
                     /translation="ESRPARARSALWEYVFFIDVEGHQKDEPVARALGELKRLAPYLK
                     VLGSYPVASN"
     gene            190..1293
                     /gene="hisC"
                     /locus_tag="VFX94_08430"
     CDS             190..1293
                     /gene="hisC"
                     /locus_tag="VFX94_08430"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX5768245.1"
                     /translation="MDLCELSPSYVRSIAPYQPGKPISELAREMGLDERSIVKLASNE
                     NPRGIGPRTRAAIEASISEIARYPDGNGFELKQALAQRYGVDMGSIVLGNGSNDVLEL
                     VALAFLGPRRAAVFSQHCFAVYPLATQARGARSIVVPAKDYGHDLEAIAKAIDDETYV
                     AWIANPNNPTGTFARYEEIEAFLKRVPERVLVVLDEAYNEYLAPELRGDTVKLIKRHP
                     NLVVTRTFSKAYGLAGLRVGYALAHASVADVMNRVRQPFNVNSVALAAAVAALDDMEF
                     VARSYAENLQGMRQLEDGAKRLRLDHIPSYGNFITLRVGKAAEIYKRLLRRGVIVRPV
                     GGGYGLPEHLRVTIGTARENERFLDALAVSLRE"
     gene            1303..2145
                     /locus_tag="VFX94_08435"
     CDS             1303..2145
                     /locus_tag="VFX94_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX5768246.1"
                     /translation="MRLAVIGVGLIGGSFALALKQAKAVSHVVGIGRNPANLKTALER
                     GIIDSIAPDVAAAARGADLVLVATPVGQYPAVLGSLRDTRALITDGGSTKRDAIAAAR
                     KTLGARISQFVPAHPIAGAEKRGAAAAAADLFKGRRVVLTPLGENSSKDISAVESAWK
                     SCGASVSRMNAEEHDAVLATVSHLPHLLAYALVHEVAGRDNSRQLFSFAAGGFRDFTR
                     IASSHPEMWRDICIANRDVLLKEVEKFSMKLEKIKKLLDSPAELERLFAEARDARDKW
                     IRSS"
     gene            2127..>2267
                     /locus_tag="VFX94_08440"
     CDS             2127..>2267
                     /locus_tag="VFX94_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX5768247.1"
                     /translation="MDSLELKPARRAAGTVRLPGSKSISNRVLLLAALARGETEVLGL
                     LDA"
BASE COUNT          389 a          776 c          744 g          358 t
ORIGIN      
        1 cgaatcgcgt ccggcgcgtg cgcgatccgc gctgtgggaa tacgtattct tcatcgacgt
       61 cgaaggccac cagaaggacg agccggtggc ccgggcgctc ggcgaactga agcggctcgc
      121 gccgtatctc aaggtcctgg ggtcgtaccc ggtggcctca aactgacaaa gggtggcgtt
      181 ttcatgctga tggatctctg cgaactttcg ccttcctacg tgcgctcgat tgcgccgtac
      241 cagcccggca agccgatctc cgagctggcg cgggagatgg gcctcgacga gcgctcgatc
      301 gtgaagcttg cgtccaacga gaacccgcgc ggcatcggcc cgcgcacgcg agccgcgatc
      361 gaggcgtcga tcagcgagat cgcgcgctac cccgacggca acggcttcga gctgaagcag
      421 gcgcttgcgc agcgctacgg cgtcgacatg ggctcaatcg tgctcggcaa cggctcgaac
      481 gacgtcctgg agctggtcgc gctcgccttc ctcggcccgc gccgggccgc ggtgttctcg
      541 cagcactgct tcgcggtgta tccgctcgcc acccaggcgc gcggcgcgcg ctcgatcgtc
      601 gtgccggcga aggactacgg ccacgatctg gaggcgatcg ccaaagcgat cgacgacgag
      661 acttatgtcg cctggatcgc gaatccgaat aacccgacgg gcaccttcgc ccgctacgag
      721 gagatcgagg cgttccttaa gcgcgtgccg gagcgcgtgc tggtagtgct cgacgaggcg
      781 tacaacgagt acctggcgcc cgagctgcgc ggcgacaccg tcaagctgat caagcgccac
      841 ccgaatctcg tcgtcacgcg cacgttctcg aaagcgtacg ggctcgctgg cctgcgcgtc
      901 ggctacgcgc tcgcgcacgc gtcggtggcc gacgtgatga accgcgtacg ccagccgttc
      961 aatgtgaaca gcgtggcgct cgcggcggcg gtcgccgcgc tcgacgacat ggaattcgtt
     1021 gcgcgcagtt atgccgagaa tctgcaaggc atgcgccagc tcgaggacgg cgcgaagcgc
     1081 ctcaggctcg accacattcc ctcgtacggc aacttcatca ccctccgcgt tggcaaggcc
     1141 gcggagatct acaagcggct gctgcgccgc ggggtcatcg tgcgcccagt cggcggcggc
     1201 tacggtttgc ccgagcacct gcgcgtgacg atcgggaccg ctcgggaaaa cgagcgcttc
     1261 cttgatgcgc tcgccgtctc actgcgggaa tgaaatgagg caatgagact cgccgtcatc
     1321 ggcgtcggcc tgatcggcgg ctcgttcgcg ctggcgctga agcaggcaaa agccgtctcg
     1381 cacgtcgtcg gcatcggccg caaccccgcc aatctcaaga ccgcgctcga gcgcggcatc
     1441 atcgattcca tcgcgccaga tgtcgccgcc gcagcacgcg gcgccgatct tgtcctggtg
     1501 gcgacgccgg tcgggcagta cccggcggta cttggctcgc tgcgcgacac gcgcgcgctc
     1561 atcacagatg gaggcagcac gaagcgcgac gcgatcgccg cggcgcgcaa aacgctcggc
     1621 gccaggatct cgcagttcgt gcccgcgcac ccgatcgccg gcgccgagaa acgcggcgcc
     1681 gcggccgcag ccgcggacct gttcaaaggc aggcgggtgg ttctgacgcc gctcggggaa
     1741 aactcgagta aagacatctc cgcggtcgag agcgcctgga agagctgcgg cgccagcgtg
     1801 agccgtatga acgccgaaga gcacgatgcg gtgctcgcca ccgtgagcca tctgccgcat
     1861 cttctcgcct acgcgctggt gcacgaggtc gccgggcgcg acaactcccg acagctcttc
     1921 tccttcgccg cgggcggctt ccgcgatttc acccgcatcg cttccagcca ccccgagatg
     1981 tggcgcgaca tctgcatcgc caaccgggat gtgcttctga aggaagtcga aaaattctca
     2041 atgaagcttg aaaagataaa gaagctgctc gacagcccgg cggagctcga gaggctgttt
     2101 gccgaagcgc gcgatgcgcg cgacaaatgg attcgctcga gctgaagccc gcgcgccgcg
     2161 ccgccggcac ggtgcggctc cccgggtcca agagcatctc caaccgcgtc ctcctgctcg
     2221 ccgcgctcgc acgcggcgag accgaggtcc tcggcctgct cgacgcc
//
LOCUS       DASUNT010000416         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_410635, whole genome shotgun sequence.
ACCESSION   DASUNT010000416 DASUNT010000000
VERSION     DASUNT010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410635"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..112
                     /locus_tag="VFX94_08445"
     CDS             <1..112
                     /locus_tag="VFX94_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX5768248.1"
                     /translation="MGQDRAVRELLSAAGLQGVASWRDLAGIPRVSGGTR"
     gene            123..440
                     /gene="grxD"
                     /locus_tag="VFX94_08450"
     CDS             123..440
                     /gene="grxD"
                     /locus_tag="VFX94_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX5768249.1"
                     /translation="MDTHERIKQQVTGNRVVLYMKGTPQFPQCGFSALAVQVLGACGV
                     RDFTAVNVLADQAIREGIKQYANWPTIPQLYVNGEFVGGSDIMREMYESGELQKLLET
                     EQA"
     gene            complement(420..1301)
                     /locus_tag="VFX94_08455"
     CDS             complement(420..1301)
                     /locus_tag="VFX94_08455"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEX5768250.1"
                     /translation="MPRALRPRFRQHPGRAAQPGQHEAHIGLGAALRQELRIESARPA
                     SGGCIHRCHAVVIGGKHRFLKTNSPDRQDLFAAEAQGLLALRNAGMRAPEPLAHGGAG
                     GEAYLLIEYLELQRPGDFAALGRMLAKAHRQPAPHFGWARDNYIGSTPQKNAWCDDWA
                     QFWRERRMRPQLEIAGLDVDVGALDQLLENHQPQPSLLHGDLWSGNVGFTQSAPVVFD
                     PAVYYGDREADLAMTELFGGFPREFYQAYAETFPLAEGYDKRKHLYNLYHLLNHLKLF
                     GRGYLGQVRSCLRLLGL"
     gene            complement(1217..>2445)
                     /locus_tag="VFX94_08460"
     CDS             complement(1217..>2445)
                     /locus_tag="VFX94_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5768251.1"
                     /translation="TSEVFLCQDPFRNRQVAVKRIFPESLRDPARGRLFRKLFFTEAS
                     LAGKLDHPHIAQIYDAGIGEDSGYIVMEYVPGGTMERYCAPEELLPIEQTVEIIFKCA
                     RALAYAHLQGVTHRDIKPGNLLYAAAPTDVRVGDFGLALNVGAETTQITGVGSPAYMS
                     PEQIREELVDHRTDLYSLGVVMYQMLCGKLPFHGSNKFSIIYQITQQEPAPPSSHRRG
                     VPEALDRIVRRAMQKDAAARYHSGDEFADDLVRALRNASGPARRDSLGDADKFAALRV
                     LGFFKRFAEAELWEVMRIGQWQRVAAGTEIVREGEPGDFFCVLLEGEARVTKNGKLIS
                     VLGPGECFGEMAYLSAERRERGATVTAARDAHIVRVPTAELERASVGCRAHFDRAFVS
                     ILVERLNRANMRLTSA"
BASE COUNT          424 a          780 c          838 g          403 t
ORIGIN      
        1 catgggacag gaccgggccg tgcgagagct gctttcggcg gcaggccttc aaggcgtggc
       61 gagctggcgg gatcttgcgg gcatcccgcg agtgagcggc ggaacgcgat aaaatccgca
      121 gcatggacac acatgagcgg atcaagcagc aggttaccgg caaccgcgtc gtgctttaca
      181 tgaagggcac gccgcagttc ccgcaatgcg gcttctcggc gcttgccgtg caggtactag
      241 gcgcctgcgg ggtgagggac ttcactgcgg tcaacgtgct cgccgatcag gcgatccgcg
      301 aaggcatcaa gcagtacgcc aactggccga ccatcccgca gctctacgtg aacggcgagt
      361 tcgtcggcgg ctcggatatc atgcgcgaga tgtacgagtc gggcgagctg cagaaactcc
      421 tagagaccga gcaggcgtaa gcacgacctg acctgcccga gatagccgcg gccgaacagc
      481 ttcaggtgat tgagcaggtg atagaggttg tagaggtgtt ttcgcttatc gtagccttcg
      541 gccagcggaa aagtttccgc gtaagcctgg tagaactcgc gcgggaatcc gccgaatagc
      601 tcggtcatcg cgagatccgc ctcgcgatca ccgtagtaca ccgccggatc gaacactacc
      661 ggcgcgctct gcgtaaagcc cacgttcccg ctccacaggt ccccgtgcag cagcgaaggc
      721 tgcggctggt ggttttccag caactggtcg agcgcgccga catcaacgtc gaggcctgcg
      781 atctcgagct gcggccgcat ccggcgctcg cgccagaact gcgcccagtc gtcgcaccag
      841 gcgttcttct gcggcgtcga gccgatgtag ttgtcgcgcg cccagccgaa gtgcggagcc
      901 ggttgtcgat gcgctttggc gagcatgcga ccgagggcgg caaagtcgcc cgggcgctgg
      961 agctcgagat attcaattag gaggtacgcc tcaccgccgg cgccgccatg cgcgagaggc
     1021 tcgggagcgc gcattccggc gttgcgcagc gcaaggagcc cctgcgcttc ggcggcgaac
     1081 aggtcttgcc ggtccggtga gttcgtcttg aggaaacggt gcttgccacc gattacgacg
     1141 gcatgacaac gatgaatgca gccgccggat gcgggccgcg ccgattcgat gcgaagctcc
     1201 tgccgcagcg ctgcgcctag gccgatgtga gcctcatgtt ggcccggttg agccgctcta
     1261 ccaggatgct gacgaaagcg cggtcgaagt gcgcgcggca gccgaccgag gcgcgctcca
     1321 gctcggcggt agggacgcgc acgatgtgcg cgtcccttgc ggcggtcacg gtcgcgccgc
     1381 gctcgcgccg ctcggccgag aggtaggcca tctcgccgaa gcattcgccc gggccgagca
     1441 cgctgatgag cttgccgttc ttggtgaccc gggcttcgcc ctcgagaagc acgcaaaaga
     1501 agtcgcccgg ctcgccttcg cgcacgatct cggtgcccgc agccacgcgc tgccactggc
     1561 cgatcctcat cacttcccaa agctccgcct cggcgaagcg cttgaaaaag ccgagcacgc
     1621 gcagtgcggc gaacttgtcg gcgtcaccca agctatcacg gcgggcgggc ccggacgcgt
     1681 tgcgtaaagc tcggaccaga tcgtcggcga actcgtcccc gctgtggtag cgcgcggccg
     1741 cgtccttttg catagcgcgg cgcacgatgc ggtcgagcgc ctctggcacg ccgcgccggt
     1801 gcgacgaagg cggcgccggc tcttgctgcg tgatctggta gatgatgctg aacttgttcg
     1861 aaccgtggaa cggcagcttg ccgcaaagca tctggtacat gactacgccg agcgagtaga
     1921 ggtcggtacg gtgatcgacg agctcctcgc ggatctgctc gggcgacatg tacgccggcg
     1981 agccgacccc ggtgatctgg gtggtctcgg cgccaacgtt gagcgcgagg ccaaagtcac
     2041 cgacgcgcac gtcggtcggc gccgcggcgt agagcaggtt gccgggcttg atgtcgcggt
     2101 gcgtgacgcc ctgcaagtgc gcgtaggcaa gggcgcgcgc gcacttgaag atgatctcga
     2161 ccgtctgctc gatcggcagc agctcctcgg gcgcgcagta gcgctccatc gtgccgccgg
     2221 gcacgtactc catcacgatg tagcccgaat cctcgccgat gccggcgtcg tagatctggg
     2281 cgatgtgcgg gtggtcgagc ttgccggcga gcgacgcctc ggtaaagaac agcttgcgga
     2341 acaggcggcc gcgcgccggg tcgcgcagcg actccgggaa aatgcgcttc accgccacct
     2401 gccggttgcg gaacgggtcc tggcagagga acacctccga ggtcg
//
LOCUS       DASUNT010000417         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_59208, whole genome shotgun sequence.
ACCESSION   DASUNT010000417 DASUNT010000000
VERSION     DASUNT010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59208"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VFX94_08465"
     CDS             complement(<1..614)
                     /locus_tag="VFX94_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX5768252.1"
                     /translation="MDRSLFRFILRYSRRDQLRIVPLVLLTMVVYYASLDLPKTIINE
                     AIQGGRFPQPDATTGLFGLQLGRLEYLLALSMVFLGLIVVNGWLKFRINTMKGWMGER
                     MLRRLRYALYDYILRFPLARFRRVKAAEMASMIKDEVEPLGGFIGEALITPLFLGGQA
                     LTAMFFILYQHWALGLIALGVVAVQAFMIPRLRKRLLVLGRERQL"
     gene            656..1504
                     /locus_tag="VFX94_08470"
     CDS             656..1504
                     /locus_tag="VFX94_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5768253.1"
                     /translation="MTFSVRFWGVRGSIACSGPRTARYGGNTSSLEVRCDERMLLFDA
                     GTGLRYLGNELIENEGVSRLASLPLEADLFLTHTHFDHVAGLPFFKPFFKSQNRFRLW
                     AGHLVEGMTLRRVLGEFMMSPLFPVPPQIFRARMEYREFKAGETLRPAEGIAVRTTLL
                     NHPDGATGYRVDYGGHSICYLTDTEHVPGAPDRNILGLIAGADLVIYDSMYTDAEYDA
                     YVGWGHSTWQEGVRLCRAAGAKRLAVFHHDPEHDDDMLDGIAREVEKELPGSIVARDG
                     LVVAVD"
     gene            1491..2147
                     /locus_tag="VFX94_08475"
     CDS             1491..2147
                     /locus_tag="VFX94_08475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5768254.1"
                     /translation="MQWIESYAAELEAIGAEPPPAPRWNQDWFPRLDAAAAYAMVRTV
                     KPRRIAEVGSGHSTRFLARAVADGGLDTRITAIDPKPRASLAGLDIEWIAAQVQRVAA
                     FPALGEGDILFIDSSHQLKPGSDVDFLLNAVLPLLPQGVRVHFHDIFLPDDYPEHWAW
                     RRYNEQPAVSALIKKNFLRVDFSSHATVKFHPEKIQGVLARVPLLPGAIESSLWLTRN
                     "
     gene            2129..>2469
                     /locus_tag="VFX94_08480"
     CDS             2129..>2469
                     /locus_tag="VFX94_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768255.1"
                     /translation="MADQELNIQPVLDWLDAGAPGARLPQDVLLHMCRRLVAQGLALV
                     RVAVFVRTLHPNVAGRGYFWREGCEEVEVDQAEHSFIGSEEHLTSPIYAVWSGNREVR
                     RRLADPTCPLD"
BASE COUNT          431 a          818 c          801 g          419 t
ORIGIN      
        1 agctggcgct cgcggccaag caccagaagg cgcttgcgca gccgcgggat catgaacgcc
       61 tgcaccgcca cgacgccgag cgcgatcagc ccgagggccc agtgctgata gaggatgaag
      121 aacatcgcgg tcagcgcctg accgccgagg aagagcggcg tgatgagcgc ctcgccgatg
      181 aagccgccga gcggctcgac ctcgtccttg atcatcgacg ccatctccgc ggccttgacg
      241 cggcggaagc gcgcgagcgg gaagcgcagg atgtagtcgt agagcgcgta gcgcagccgc
      301 cgcagcatgc gctcgcccat ccagcctttc atcgtgttga tgcggaactt gagccagccg
      361 ttcaccacga tgaggccgag gaacaccatc gacagcgcca agagatattc gagccgcccg
      421 agctgcagtc cgaataaccc tgtcgtcgcg tcgggctgcg ggaagcgtcc gccctggatc
      481 gcctcgttga tgatggtctt gggcagatcg agcgacgcgt aatagaccac catcgtcagc
      541 agcacgagcg gcacgatgcg cagctggtcg cgcctgctgt atctgaggat gaagcggaaa
      601 aggctgcggt ccacgggggt cccctagggc ggcatcttac aagctatagt tcagcatgac
      661 tttcagcgtt cgcttctggg gcgtacgcgg ctcgattgcc tgctccggcc cgcgcacagc
      721 gcgctacggc ggcaacactt cttcgctgga agtgcgctgc gacgagcgaa tgctgctctt
      781 cgacgcgggc acggggctgc gatacctcgg aaacgagctg attgagaacg aaggcgtatc
      841 ccggctcgca tctcttccac tggaggcgga tctcttcctc acgcacaccc atttcgacca
      901 cgtcgccggg ctgcctttct tcaagccgtt tttcaagtcg cagaaccgct tccggctctg
      961 ggcggggcac ctggtggagg ggatgacgct gcgccgagtg ctcggcgagt tcatgatgtc
     1021 gccgctcttt ccggtgccgc cgcagatatt ccgcgcgcgc atggagtacc gcgaattcaa
     1081 ggcgggcgag acgttgcgtc ccgccgaggg catcgcggtg cgcaccacgc ttctcaacca
     1141 tcccgacggc gccaccggct accgcgtcga ctacggcggg cactcgatct gctacctcac
     1201 cgacaccgag cacgtgcccg gcgcgccgga ccgcaacatt ctcggcctca tcgcgggcgc
     1261 cgacctcgtg atctacgaca gcatgtacac ggatgccgag tacgacgcgt acgtgggatg
     1321 gggacattcg acgtggcagg aaggcgtgcg gctgtgccgc gccgcgggcg cgaagcgcct
     1381 tgcggtgttc caccacgacc ccgagcacga cgacgatatg ctcgacggca tcgcgcgcga
     1441 ggtggagaag gagctgcccg ggtccatcgt cgcgagggac ggcctggttg ttgcagtgga
     1501 ttgaaagcta cgccgccgag ctcgaagcga taggcgcgga gccgccgccg gctccgcgct
     1561 ggaaccagga ctggtttccg cgcctcgacg ccgccgccgc ttatgcgatg gtgcgcaccg
     1621 tgaagccccg gcgcatcgcc gaggtcggct cggggcactc gacgcgcttc ctcgcgcgcg
     1681 cggtcgccga cggcggcctc gatacgcgca tcaccgccat cgatccgaaa ccaagagcca
     1741 gtctggcggg gctcgacatc gaatggattg cagcccaggt gcagcgcgtc gcggcgtttc
     1801 ctgcgctcgg cgaaggcgac atccttttca tagactcgag ccaccagctc aagcccggaa
     1861 gcgacgtcga ttttctgctc aacgcagtgc tgcctctcct gccgcagggc gtgcgcgtgc
     1921 atttccacga catcttcctg cccgacgact atcccgagca ctgggcctgg cgacgctaca
     1981 acgagcagcc tgcggtttct gccttgatca agaaaaactt tctgcgcgtt gatttctcga
     2041 gccatgccac cgtcaagttt catcccgaga aaatccaggg cgtcctcgcg cgcgtgccgc
     2101 tcctgcccgg cgccatcgag tcgagcctgt ggctgaccag gaactgaaca tccagccggt
     2161 gctcgactgg ctcgacgccg gagcgccggg ggcgcggctg ccgcaggacg tgctgctaca
     2221 catgtgcagg cgactcgtgg cgcaggggct tgcgctcgtg cgcgtcgccg tctttgtgcg
     2281 cacgctgcat ccgaacgtgg cgggacgagg ctacttctgg cgcgaagggt gcgaggaagt
     2341 agaggtcgac caagccgagc acagcttcat cggttccgaa gagcatctaa cgagcccaat
     2401 ctatgctgtc tggtcgggca atcgcgaggt gcgccgccgc ctggccgatc ccacctgccc
     2461 cttggacta
//
LOCUS       DASUNT010000418         3901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_673033, whole genome shotgun sequence.
ACCESSION   DASUNT010000418 DASUNT010000000
VERSION     DASUNT010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673033"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFX94_08485"
     CDS             <1..204
                     /locus_tag="VFX94_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768256.1"
                     /translation="GPQAPAQPAAGGPQPPASAAVGGPMGKRHGGGGRSAEYDQRLRA
                     LEADLLAAQQRFDAALRRYNSLR"
     gene            complement(186..1250)
                     /locus_tag="VFX94_08490"
     CDS             complement(186..1250)
                     /locus_tag="VFX94_08490"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX5768257.1"
                     /translation="MFRTPQIDDLNVTSQVRLVSPEALHDEIPLSDAARRNVLAGRDT
                     IKRILERSDPRRFVVVGPCSIHDPEAALDYGRRLAALTREVEDTFFLVMRVYFEKPRT
                     TVGWKGLINDPDLDDSFDIEKGLRIARKLLVDLNELGLPCGTEALDPITPQYIADLVS
                     WSAIGARTTESQTHREMASGLSMPVGIKNGTDGNRAIAINALLSVSKPHAFLGIDPEG
                     RCALTYTRGNRHAHIVLRGGTAPNYDTVSVALTEEELNKAGLPCAIMIDCSHGNSGKD
                     PARQPLVFGDVIHQIVEGNRSIIGAMIESNLEWGNQPLAERSKLRYGVSITDACIDWP
                     TTEKTLREARARLRSQRREL"
     gene            complement(1479..1928)
                     /locus_tag="VFX94_08495"
     CDS             complement(1479..1928)
                     /locus_tag="VFX94_08495"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX5768258.1"
                     /translation="MLKALKRALGAALSEGEPVPAQERRQLELAVAVLVHEARRADYV
                     EGDAKESDAGVSALCDILSLERAQAAELLAQGREKAQHLTSFYAPVTTIKREFSMPER
                     IRLVEHLWRVSFADGKLDYYEDHYVRKIAHLLYVPNTDSMLARNRAR"
     gene            complement(1995..2237)
                     /locus_tag="VFX94_08500"
     CDS             complement(1995..2237)
                     /locus_tag="VFX94_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEX5768259.1"
                     /translation="MALLINEACTLCDACVPVCPNEAIKAGEPYVIDPLKCTECVGAK
                     DEPQCKLVCPSDCIVKNPDFDETPEQLQEKYAALHP"
     gene            complement(2250..3080)
                     /gene="bcrD"
                     /locus_tag="VFX94_08505"
     CDS             complement(2250..3080)
                     /gene="bcrD"
                     /locus_tag="VFX94_08505"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit D"
                     /protein_id="HEX5768260.1"
                     /translation="MTYTVGIDIGSGAVKTVLFRDAEWLAKRVERIRRRDPMELARLG
                     FEEVIKEQDLEEKDVSYTATTGEGENVKFATGHFYSMTTHARGGVFLNPEARAVVDIG
                     ALNGRAIYIDERGKVLAYKMTSQCASGSGQFLENIARYLGVAVEEIGPLSKSSKSPEK
                     VSSICAVLAETDVINMVSRGIATPDILKGIHISMASRLVKLLKVTGITKGTVLLTGGL
                     ALDEGLLAALQEELVNEKISGLTAVSHPDSIYAGAIGAALWGAFRFRKLKELQERIAA
                     "
     gene            complement(3077..>3901)
                     /locus_tag="VFX94_08510"
     CDS             complement(3077..>3901)
                     /locus_tag="VFX94_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX5768261.1"
                     /translation="SIIEVENRVYGDSIRRHLIAALERTFAALPETAGRREAVEAPIQ
                     GILDTELEETYVVGTGYGRARLPFSKEHIRSEILCHGLGAHVMYPATATVLDIGGQDT
                     KAIQVDSHGIVESFQMNDRCAAGCGRYLGYIADEMNMGLHELGPLAMKATKTIRINST
                     CTVFAGAELRDRLALGEKREDIMAGLHRAIILRAMSILARSGGIRNEFTFTGGVAKNE
                     AAVKSLTGLVKENYGEMTININPDSIYTGALGAATFAWRAVVEEGKTAEHKERQAA"
BASE COUNT          591 a         1259 c         1331 g          720 t
ORIGIN      
        1 gggccgcagg ctccggcaca gccggccgcg gggggaccgc aaccgccggc gtctgcggcg
       61 gtcggcgggc cgatgggcaa gcgccatggc ggaggcggaa ggtccgcgga atacgaccag
      121 cgcctgaggg cgctggaagc cgacctgctc gcggcgcagc agcgctttga cgccgcgttg
      181 aggcgctaca actccctgcg ctgactgcgc agcctcgcgc gcgcctcgcg cagcgtcttt
      241 tcggtggtgg gccagtcgat gcaggcgtcg gtgatcgata cgccgtagcg cagcttcgag
      301 cgctcggcga gcggctggtt gccccactcg aggttcgatt cgatcatcgc gccgatgatg
      361 gagcggttgc cttcgacgat ctggtggatc acgtcgccga agaccagggg ttggcgcgcg
      421 gggtccttgc cgctattacc gtgactgcag tcgatcatga tggcgcacgg cagccctgcc
      481 ttgttcaatt cttcttcagt caaggccaca gaaacagtgt cgtagttcgg tgccgtgccg
      541 ccacgcagga cgatgtgggc gtgccggttg ccccgggtgt aggtgagcgc gcagcggccc
      601 tcggggtcga taccgaggaa ggcgtggggc ttggagaccg agaggagcgc gttgatggcg
      661 atggcgcggt tgccgtcggt gccgttcttg atgccgaccg gcatggacag gccgctcgcc
      721 atctcgcggt gggtctggga ctcggtggtg cgggcgccga tcgcgctcca ggaaacgagg
      781 tcggcgatgt actgcggcgt gatcggatcg agcgcttcgg tgccgcaggg caagccgagc
      841 tcgttcagat cgacgagcag cttgcgcgcg atgcgcaggc ctttctcgat gtcgaacgag
      901 tcatcgaggt cggggtcgtt gatcaggcct ttccagccga ccgtggtgcg cggcttctcg
      961 aagtaaacgc gcatcacgag gaagaaagtg tcctccacct cgcgcgtgag cgcggcgagg
     1021 cgccggccgt aatcgagcgc cgcctccggg tcgtggatcg agcacgggcc gaccacgacg
     1081 aaacgtcgcg ggtcgctgcg ttcgaggatc ctctttatcg tgtcgcggcc ggccaggacg
     1141 ttgcgacgcg ccgcatcgga cagcggaatc tcgtcgtgca gcgcctcagg cgaaacgagc
     1201 cggacctggc tcgtgacgtt gaggtcatcg atctgcgggg tgcgaaacat ggctccccgt
     1261 attctgtcac cgggaacagc ctagttccgc cgggcgcggc tcctgagata ctcgaaatag
     1321 cgcacgttgg cgcagcacgg gtggcgccca aacctgccgt gccacttcat ccagcggatg
     1381 caggcgtcgg tgtccccgca atggttgcag cgctgcgcgg cctgcgcgag ctccgcgaag
     1441 tcgccgccgg gcggcgcggc ttgatctagc gcaacccttt accgggcgcg gttgcgcgcg
     1501 agcatgctgt cggtgttcgg gacgtagagc agatgcgcga tcttgcgcac gtaatggtcc
     1561 tcgtaatagt cgagcttgcc gtcggcgaac gagacacgcc acaggtgctc gacgaggcga
     1621 atgcgctcgg gcatcgaaaa ctcacgcttg atggtcgtta cgggtgcgta gaagctggtg
     1681 aggtgctgcg ccttctcgcg cccctgcgcg aggagttctg cggcctgcgc gcgctccagg
     1741 ctgaggatgt cgcagagcgc cgacacaccc gcgtcggact ccttggcgtc gccttcgacg
     1801 tagtccgcgc ggcgcgcctc gtggaccagg acggcgacgg caagctcgag ctgccggcgc
     1861 tcttgcgccg gtaccggttc accttcgctg agtgccgcgc caagcgcgcg cttcagcgct
     1921 ttgagcatgg gacttcatac tacgtccccc gacgcgccga gcgcttgcga ttttcaggct
     1981 tgatgcaaat cggctcaagg atgaagcgcg gcgtactttt cctggagctg ctcgggcgtt
     2041 tcgtcgaagt ccggattctt gacgatgcag tccgacggac agaccagctt gcactgcggc
     2101 tcgtccttcg cgccgacgca ctcggtgcac ttcagcggat cgatcacgta aggctcgccc
     2161 gccttgattg cttcgttcgg gcacaccggg acgcaggcgt cgcacagcgt gcaggcttca
     2221 ttgatcagca gtgccatggg aggtcccttt caggcggcga tccgctcctg gagttccttc
     2281 agttttctga accggaacgc accccagagc gcggcgccga tggcgccggc gtagatcgag
     2341 tcggggtggc ttacggcggt aaggccgctg atcttctcgt tcacgagctc ctcctgcagc
     2401 gcggcgagca ggccctcgtc gagggcgagg cccccggtga ggaggaccgt acccttggtg
     2461 atgccggtga ccttcagcag cttcaccagc cgcgaagcca tcgagatgtg gatgcccttc
     2521 aggatgtcgg gcgtggcgat gccgcgcgac accatgttga tgacgtcggt ctcggcgagc
     2581 acggcgcaga tcgagcttac cttctccggg ctcttcgagc tcttcgagag cggcccgatc
     2641 tcctcgaccg cgacgccgag atagcgcgcg atgttctcga ggaactggcc cgagcccgag
     2701 gcgcactggc tggtcatctt gtaggcgagc accttgccgc gctcgtcgat gtagatcgcg
     2761 cggccgttca gcgcaccgat atcgaccacc gcgcgcgcct cgggattgag gaatacgccg
     2821 ccgcgcgcat gcgtggtcat cgagtagaag tgaccggtgg cgaacttgac gttctctccc
     2881 tccccggtcg tcgcggtgta cgagacgtcc ttctcctcca gatcctgctc cttgatgact
     2941 tcctcaaagc ccagccgcgc caattccatc ggatcgcggc ggcggatgcg ctcgacacgc
     3001 ttcgcaagcc attccgcgtc gcggaaaaga acggtcttga ccgcgcccga accgatatcg
     3061 atgccaacgg tgtatgtcat gccgcctgtc tttccttgtg ttccgcggtc ttgccttcct
     3121 ccacgaccgc gcgccacgcg aaggtggccg cgccgagcgc cccggtgtag atcgaatcgg
     3181 ggttgatgtt gatcgtcatt tcgccgtagt tttccttcac caggccggtg agcgacttca
     3241 ccgccgcctc gttcttcgcc acgccgccgg tgaaggtgaa ctcgtttcgg atcccgcccg
     3301 agcgcgccag gatcgacatc gcgcgcagga tgatggcgcg gtgcagcccg gccatgatgt
     3361 cctcgcgctt ctcgccgagc gcgaggcggt cgcggagctc ggcgccggcg aacacggtgc
     3421 aggtcgaatt gatgcggatg gtcttcgtcg ccttcatcgc gagcgggccg agctcgtgca
     3481 gccccatgtt catctcgtcg gcgatgtagc ccaggtagcg cccgcagccc gcggcgcagc
     3541 ggtcgttcat ctggaacgac tcgacgatgc cgtggctgtc gacctggatc gccttggtgt
     3601 cctggccgcc gatgtcgagc acggtggcgg tcgccgggta catcacgtgc gcgcccaggc
     3661 cgtggcacag gatctcggag cggatgtgct ccttcgagaa cggcagccgc gcgcgcccgt
     3721 agccggtgcc caccacgtac gtctcctcga gctcggtgtc gaggatcccc tggatcggcg
     3781 cctccaccgc ctcgcgccgg ccggcggtct cgggcagcgc ggcgaacgtg cgctcgagcg
     3841 cggcgatcaa gtgacggcgg atcgagtcgc cgtacacgcg gttctccacc tcgatgatcg
     3901 a
//
LOCUS       DASUNT010000419         3865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_148202, whole genome shotgun sequence.
ACCESSION   DASUNT010000419 DASUNT010000000
VERSION     DASUNT010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148202"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="VFX94_08515"
     CDS             <1..1121
                     /locus_tag="VFX94_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5768262.1"
                     /translation="KEMLRASLTPGPHLAGPDDLAVMPYTSGTTGRPKGCMHTHANVQ
                     ATTVPYLHWRGAQDHAVVLCAMPMFHVTGMQAGMNAPIHIGATLVVLSRWDRECAAMQ
                     IERARVTNWSAITTMLVDFLSNPKLSQYDLSSLRVLGGGGAAMPEALARRLEQVIGLP
                     FVEGYGLSETMAPTHINPPHRPKRQCGGIPIHNTDARVLDLQDSHELGPHEVGEIVVH
                     GPQVFRGYWNQPEATAQAFLEHDGKRFFRTGDLGYYDEEGYFFITDRLKRMINCSGFK
                     VWPAEVESMLYAHPAIQEACVIGTPDGYRGETVKAVVVLKSSFRSTSSKDIIEWARGR
                     MAAFKVPRVVEFVEELPKTATGKILWRRLQEDENRKAP"
     gene            1118..1576
                     /locus_tag="VFX94_08520"
     CDS             1118..1576
                     /locus_tag="VFX94_08520"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX5768263.1"
                     /translation="MRARLFALFLALGQAGLAWPHSHEKGEIQVRHPWSRATPPGAQV
                     AVGYMEIRNHGAQPDRLVAVSTPVAKRVEMHVTQREGEVMRMRQVKHFEIPARERVAL
                     RPGGSHLMLVDITQPLKKGERFTMRLHFERAGELDIELDVQEQGSRHARH"
     gene            complement(1583..2716)
                     /locus_tag="VFX94_08525"
     CDS             complement(1583..2716)
                     /locus_tag="VFX94_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX5768264.1"
                     /translation="MGPLQGIRVLEFESIGPAPFAGMLLADMGADVLVIDRPAETDLG
                     LKRERRHDVMMRGKRSVTLNLKGENAKAAVLELVCRADALIEGMRPGVMERLGVGPDE
                     ALRKNPTLVYGRMTGWGQDGPLAPRAGHDINYIALAGVLHAFGRRGEAPVPPLNVVGD
                     FGGGGMLLAFGVACALLEAARSGRGQVIDAAMVEGASLLAVMFAGFLGGGSWSEQRGT
                     NILDTGAPWYNVYETKDGKYVSIGSIETRFYQELLQKLGVPDLGQHERARWPEMHSLF
                     SNAFKSKTREEWCKLFEGSDACFAPVLPWSEARRDPHMAFRKNFIEAAGVEQPAPAPR
                     FSRTPGAVRRAPPERGEGGGAALREWGFAAADIARLRALGLGC"
     gene            2733..3548
                     /locus_tag="VFX94_08530"
     CDS             2733..3548
                     /locus_tag="VFX94_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX5768265.1"
                     /translation="MPARCPERIVCLTEEGTETLYLLGEERRIVGISGYTVRPPRARR
                     EKPRVSAFLSAKTDKILELKPDLVIGFSDLQADIARELAKAGLNVLIFNQRSVQEILD
                     MIGMLASIVGEPDKGRGLIALLEKNLADIRESARAFSRRPRVYFEEWDEPMISGIRWV
                     SELVDIAGGEDVFAEQSRSQAAAGRIVSDAKIVVDKDPEIILGSWCGKKFRPERVAAR
                     PGWERISAVKENQLFEVKSADILQPGPAALTDGVKCIHELLAGYLKRSSSRGS"
     gene            complement(3520..>3865)
                     /locus_tag="VFX94_08535"
     CDS             complement(3520..>3865)
                     /locus_tag="VFX94_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX5768266.1"
                     /translation="DGYVHFIPNGEIKTVSNRTRMYAFANVDVGVPDEADCDAVFKAM
                     RDVGAALRADTSFARLILDDTEVLGVERWELWGMMLRCRLKVLAHERDNVRREFSRRL
                     AIEFRTRGLKTA"
BASE COUNT          652 a         1308 c         1300 g          605 t
ORIGIN      
        1 ggaaggaaat gctgcgcgcg agccttacgc cgggcccgca cctcgccggc ccggacgatc
       61 tcgcggtcat gccgtatacg tcgggcacga ccggccggcc gaaaggctgc atgcataccc
      121 atgcaaatgt gcaggcaacg accgtgcctt accttcactg gcgcggcgcg caggaccacg
      181 cggtcgtgct ttgcgcgatg ccgatgttcc acgtcaccgg catgcaggcg ggcatgaacg
      241 cgccgatcca tatcggcgcc acgctggtgg tgctgtcgcg ctgggaccgc gagtgtgccg
      301 cgatgcagat cgagcgcgcg cgcgtcacca actggtcggc gatcaccacc atgctggtgg
      361 atttcctctc caatccgaaa ctcagccagt acgatttgtc gtcgctacgc gtgctcggag
      421 gcggaggagc ggcgatgccc gaagcgctcg cgcggcggct ggagcaggtc atcggactgc
      481 ccttcgtcga aggctacggt ctctccgaga ccatggcgcc gacgcatatc aatccgccgc
      541 atcggccgaa gcggcagtgc ggcggcatcc cgatccacaa caccgacgcg cgggtgctcg
      601 acctgcagga ttcccacgag ctcgggccgc acgaggtggg cgagatcgtg gtccacggac
      661 cgcaggtgtt ccgcggctac tggaaccagc cggaggccac cgcgcaggcg ttcctcgagc
      721 acgacggcaa gcgcttcttc cgcaccggcg acctcggcta ctacgacgag gagggctact
      781 tcttcatcac cgaccggctg aagcgcatga tcaactgctc gggcttcaag gtctggccgg
      841 ccgaggtgga gtcgatgctc tacgcgcacc cggcgatcca ggaggcctgc gtcatcggca
      901 cgcccgacgg ctaccgcggc gagacggtca aggccgtggt cgttctgaag agcagcttca
      961 gaagcacctc gtccaaggac atcatcgagt gggcgcgcgg ccgcatggcg gcgttcaagg
     1021 tgccccgggt ggtcgagttc gtcgaggagc tgcccaagac cgcgaccggc aagatcctgt
     1081 ggcgcaggct gcaggaggac gagaaccgca aggcgccatg agggcccggc tcttcgctct
     1141 attcctcgcg cttggacagg cggggctcgc atggccccat tcccatgaaa agggcgagat
     1201 ccaggtgcgc cacccctggt cgcgcgccac gccgcccggc gcccaggtcg cggtgggcta
     1261 catggaaatc cgcaaccacg gcgctcagcc cgaccggctg gtcgcggtat cgacaccggt
     1321 cgccaagcgc gtcgagatgc acgtcaccca gcgtgagggc gaggtcatgc gcatgcggca
     1381 ggtaaagcat ttcgagattc ctgcgcgcga gcgcgtcgcg ctgcgccctg gcggctcgca
     1441 cctgatgctc gtggacatca ctcagccgct caagaagggc gagcggttca cgatgcggct
     1501 gcacttcgag cgcgcaggcg agctcgacat cgagctcgac gtgcaggagc agggctcccg
     1561 gcacgcgcgt cactgaacgg cctcagcagc ccagtccgag cgcgcgcagc cgcgcgatgt
     1621 cggcggccgc gaatccccat tccctgagcg ctgctcctcc gccctcgccg cgctcgggcg
     1681 gcgcgcgccg caccgcgccc ggcgtgcgcg agaagcgcgg cgcgggcgcg ggctgctcca
     1741 cgccggcggc ttcgatgaaa ttctttctga aagccatgtg cggatcgcga cgcgcttcgg
     1801 accacggcag caccggcgcg aagcatgcgt ccgagccctc gaacagcttg caccattctt
     1861 cccgggtctt gcttttgaaa gcattggaaa acaaagaatg catctcgggc cagcgggcgc
     1921 gctcgtgctg gccgagatcg ggcacgccga gcttctgcag cagctcctgg tagaaccgcg
     1981 tttcgatcga gccgatcgag acgtacttgc cgtccttcgt ctcgtagacg ttgtaccagg
     2041 gcgcgccggt atcgaggatg ttggtgccgc gctgctccga ccagctgccg ccgccgagga
     2101 aaccggcgaa catcaccgcg agcagcgacg cgccttccac catcgcggcg tcgatcactt
     2161 ggccacggcc agagcgcgcc gcctcgagca gcgcgcaggc gacgccgaag gcgagcagca
     2221 tgccgccgcc gccgaagtcg ccgacgacgt tgagcggcgg caccggcgcc tcgcccctgc
     2281 gcccgaacgc gtgcagcacg ccggcgagcg cgatgtagtt gatgtcgtgc ccggcgcgcg
     2341 gcgcgagcgg cccgtcctgg ccccagccgg tcatccgccc gtagaccagc gtgggatttt
     2401 tcctgagcgc ctcgtcgggg ccgacgccga ggcgctccat gaccccgggt cgcatgcctt
     2461 cgatcaacgc atcggcccgg caaacgagct cgagaacggc cgcctttgcg ttctcgccct
     2521 tcagattcaa cgtcacggag cgcttgccgc gcatcatcac gtcgtggcgg cgttcgcgct
     2581 tgagcccgag atcggtctcg gcggggcggt cgatcaccag cacgtcggcg cccatgtcag
     2641 cgagcagcat gccggcgaag ggcgccggcc cgatcgactc gaactcgagc acgcggatgc
     2701 cctgcaaggg tcccatgatt cgataataca gaatgccggc gcgttgcccc gagcgcatcg
     2761 tgtgcctgac cgaggaaggc accgagacgc tgtacctcct cggcgaggag cgccgcatcg
     2821 tcggcatctc gggctatacg gtgcgcccgc cgcgcgcccg gcgcgagaag ccgcgcgttt
     2881 cggcctttct ctcggcgaag accgacaaga tcctcgagct caaaccggac ctcgtcatcg
     2941 gcttctccga tttgcaggcc gacattgcgc gcgagctcgc caaggcgggc ctgaacgtgc
     3001 tgatcttcaa ccagcgctcg gtccaggaga tcctcgacat gatcggcatg cttgcctcga
     3061 tcgtcggcga gccggacaag gggcgcgggc tgatcgcttt gctggaaaag aatcttgcgg
     3121 atatccggga gagcgcaaga gcgttctcca ggcggccgcg cgtctatttc gaggaatggg
     3181 acgagcccat gatcagcggc atccgctggg tgagcgagct cgtcgacatc gccggcggcg
     3241 aggatgtgtt cgcggagcag tcgcgctcgc aggccgcggc ggggcgcatc gtttccgatg
     3301 cgaaaatcgt ggtggacaaa gaccccgaga tcatcctcgg ctcctggtgc ggaaagaagt
     3361 tcaggccgga gcgcgtcgcg gcgcgccccg gctgggagcg tatttcagcc gtgaaggaga
     3421 atcagctgtt cgaggtgaag tcggccgaca tcctgcagcc cggaccggcg gcgctgaccg
     3481 acggcgtgaa gtgcatccac gagcttctcg ccgggtacct caagcggtct tcaagccgcg
     3541 ggtcctgaat tcgatcgcga gccggcggct gaattcgcgg cgcacgttgt cgcgctcgtg
     3601 cgccagtacc ttcaagcggc agcgcagcat catgccccag agctcccagc gctcgacgcc
     3661 gagcacctcg gtatcgtcca ggatcagccg ggcgaagctg gtgtcggcgc gcagcgccgc
     3721 gccgacatcg cgcatggcct tgaagaccgc gtcgcagtcg gcttcgtcgg gcacgccgac
     3781 gtcgacgttg gcgaaagcgt acatgcgcgt gcggttggag acagtcttga tctcgccgtt
     3841 cgggatgaaa tgcacgtagc cgtcg
//
LOCUS       DASUNT010000420         3259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_148666, whole genome shotgun sequence.
ACCESSION   DASUNT010000420 DASUNT010000000
VERSION     DASUNT010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148666"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="VFX94_08540"
     CDS             complement(<1..1537)
                     /locus_tag="VFX94_08540"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX5768267.1"
                     /translation="MNEPTLAARTVTELKEHIVEVISDSGEGAQRCGQSLGSIAARMG
                     IGIWTVEIIPAEIRPPARSVAGASGNRIRLGAGRVTNGGDLTNLVVAFNEQALLGRVR
                     AGELAPGCTILLESMWREHRDPAIRESYEKAVASLRSAKYDVIEIPMGRECNAVVPNA
                     EKGKNMFALGMLCSIYSLELALAREQIALTFGKKSQAVIDSNVKLLEAGYRWGEKNLD
                     FKYRIPAVRPKEPQVVVNGNTALALGVLASGMEICAMYPITPATSASHYLSDVFEKVG
                     GVVHQAEDEIAACAFAIGASYAGKCTVTITSGPGYSLKQEAIGLAVMSEIPLVVINVQ
                     RGGPSTGQPTKAEQGDLMSAIFGSHGDAPKVVMAASTIEDCFYSVITARRIAETFNMV
                     VVVLTDASLATSQQPFPRPQFDAAWMAPPADQSTVPEGLKPYDWDAATGLARRFIPGQ
                     PNGMHTVTGLAHDRDSHVAYDPEINEESIHFRSLKLAALQRTLEAPPFFGPPQGDLLV
                     IGWG"
     gene            complement(1537..3018)
                     /gene="gltA"
                     /locus_tag="VFX94_08545"
     CDS             complement(1537..3018)
                     /gene="gltA"
                     /locus_tag="VFX94_08545"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HEX5768268.1"
                     /translation="MAKKTIRTIPQERTPMPERPAKERAKSFQEVACGYTLENALREA
                     ERCLLCPDPKCIAGCPVNINIPEFIRRITVRDFRGAYDTIAETNLLPAICGRVCPQES
                     QCEGVCTVGDTLEPVAIGRLERWVGDKALAEGWSSTPYVEPKGFRIGIVGSGPAGVAC
                     AADMAKAGCEVTVYEAFQEPGGVLKYGIPDFRLPNPVIDAESENLRKLGVQFECNTLV
                     GRLFTIEQMIDEMGFHAVFIGTGAGYPAMMGIPGESLNGVLSANELLTRCNLMRARDF
                     PKFDTPLPAAKKVAVIGSGNTAMDALRVSLRLGAEKVHCIYRRSRAESPARAEELHHA
                     EEEGIEFHWLTNPLEILDDGKGNVRGMRCIRMKLGEPDSSGRPRPEPVPGSEFEFAAD
                     MVVYAIGTNANPIIGQTTKISLNKRGYLAVDDDLATSLAGVFAGGDIVTGAATVIEAM
                     GAGRKAARSMKKYLGLYEKGSKNEEKLFGIPVDQHNFVRLRAA"
     gene            complement(3011..>3259)
                     /locus_tag="VFX94_08550"
     CDS             complement(3011..>3259)
                     /locus_tag="VFX94_08550"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768269.1"
                     /translation="DGTGMCGGCRVKVGDRVKFACVDGPDFDGHQVDFDDLMARLKRF
                     DEQEKAAMKRFADSCRMKAPPQPESLDLPDPFEEVRGG"
BASE COUNT          487 a         1073 c         1088 g          611 t
ORIGIN      
        1 tgccccagcc gatcaccagg aggtcgcctt gcggcgggcc gaaaaacggc ggcgcctcta
       61 gcgtgcgctg cagcgcggcg agcttcaggc tccggaagtg gatcgattct tcgttgatct
      121 ccgggtcata ggcgacatgg ctgtcgcggt cgtgcgcgag gccggtaacc gtgtgcatgc
      181 cgttcggctg ccccgggatg aagcgccgcg caagcccggt agcggcgtcc cagtcgtagg
      241 gcttgagccc ctcgggaacc gtgctttggt cggccggcgg cgccatccac gcggcgtcga
      301 actgcgggcg cgggaagggc tgctgtgagg tggcgaggct cgcgtcggtc aggacgacca
      361 cgaccatgtt gaaggtctcg gcgatgcgcc tagccgtgat caccgaatag aagcagtcct
      421 cgatcgtgct cgccgccatc accaccttcg gcgcgtcgcc gtggctgccg aagatcgcgc
      481 tcatcaggtc gccttgctca gccttggtcg gctggccggt gctcgggcca ccgcgctgca
      541 cgttgatcac cacgagcggg atctcgctca tcaccgcgag cccgatcgct tcctgcttga
      601 gcgaatagcc cgggccggag gtgattgtca cggtgcactt gccggcgtag gaggcgccga
      661 tcgcgaacgc gcacgccgcg atctcgtctt cggcctggtg gaccacgccg cccactttct
      721 cgaacacgtc cgagagatag tgcgacgctg aagtcgccgg cgtaatcggg tacatcgcgc
      781 agatttccat ccccgaggcg agcacgccga gcgcgagagc ggtgttgccg ttcaccacga
      841 cctgcggttc cttcggccgc acggctggga tgcggtactt gaagtcgagg ttcttctcgc
      901 cccagcggta gcccgcctcg agcagcttga cgttggagtc gatgacggct tgagacttct
      961 ttccgaaggt gagcgcgatc tgctcgcgcg cgagggcgag ctcgagactg tagatgctgc
     1021 acagcatccc cagcgcgaac atgttcttgc ccttttccgc gttggggacg acggcattgc
     1081 attcgcgccc catggggatc tcgatgacgt cgtacttggc ggaacgtaac gaggcgaccg
     1141 ccttttcgta gctttccctt attgcggggt ccctgtgctc gcgccacatg ctttcgagca
     1201 ggatggtgca gcccggggcg agctcgcccg cgcgcacgcg gccgagcaac gcctgctcgt
     1261 tgaaggcgac tacgaggttg gtgaggtcgc cgccattggt gacgcgcccg gcaccgagcc
     1321 ggatgcggtt gccgctcgcg ccggcgaccg agcgcgcggg cgggcggatc tcggccggga
     1381 tgatctccac cgtccagatg ccgatgccca tgcgcgcggc gatcgagccg agcgactggc
     1441 cgcagcgctg cgccccttcg cccgaatcgc tgatcacctc gacgatgtgc tccttgagct
     1501 cggtgacggt cctcgcggcg agggtcggtt cgttcatcag gcggctcgca gtcgcacgaa
     1561 gttatgttgg tcgacgggaa taccgaacag cttttcttcg ttcttcgagc ctttttcgta
     1621 aagacccaga tacttcttca tgctgcgcgc ggccttgcgc ccggcgccca tcgcctcgat
     1681 caccgtggcg gcgccggtga cgatgtcccc tccggcgaaa acgcctgcaa gggaggtcgc
     1741 aagatcatca tcgaccgcca gatagcctct cttgttgaga gaaatcttcg tggtctggcc
     1801 gatgatcggg ttggcgttgg tgccgatcgc atacaccacc atgtcggcgg cgaactcgaa
     1861 ctcgctgccg gggacaggct cgggccgcgg gcgcccggag gagtcgggct cgccgagctt
     1921 catgcggatg cagcgcatgc cgcgcacgtt gcccttgccg tcgtccagga tctcgagcgg
     1981 gttggtgagc cagtggaact cgatgccttc ctcctcggcg tggtgcaact cttcggcgcg
     2041 cgccgggctc tcggcgcggc tgcgccggta gatgcagtgc accttctcgg cgccgaggcg
     2101 caggctcacg cgcagcgcgt ccatcgcggt attgcccgag ccgatgaccg cgactttttt
     2161 cgccgcgggg agcggtgtat cgaatttggg gaagtcgcgc gcgcgcatca ggttacagcg
     2221 cgtaaggagc tcgttcgcgg aaagcacgcc gttcagcgac tcgcccggga tccccatcat
     2281 cgcgggataa cccgcgccgg tgccgatgaa gaccgcgtgg aagcccatct cgtcgatcat
     2341 ctgctcgatg gtgaagagac ggcccaccag cgtgttgcac tcgaattgca cgccgagctt
     2401 gcgcaggttc tcgctctcgg catcgatcac cgggttcggc aggcggaagt cgggaatgcc
     2461 gtacttgagg acgccgccgg gctcctggaa cgcttcgtag accgtcacct cgcagcccgc
     2521 cttggccatg tcggcggcgc aggccacgcc tgccggtcct gagccgacga tgccgatccg
     2581 aaagcctttc ggctccacgt acggagtgct cgaccagccc tcggcgagcg ccttgtcgcc
     2641 cacccagcgc tcgagccggc cgattgccac cggctccagc gtatcgccca ccgtgcatac
     2701 cccttcgcat tggctttcct gcgggcacac gcgcccgcag atcgccggca gcaggttcgt
     2761 ctccgcgatg gtgtcgtagg cgccgcgaaa atcgcgcacg gtgatgcggc ggatgaattc
     2821 cgggatgttg atgttgaccg ggcagccggc gatgcacttc gggtcagggc agagaaggca
     2881 tcgctcggct tcgcgcagcg cgttctcgag cgtgtagccg caggcgactt cctggaagct
     2941 cttcgcgcgc tcctttgccg gccgctcagg catcggcgtg cgctcctgcg gaatggtgcg
     3001 gatggttttc ttagccaccg cgcacttctt cgaacgggtc gggcaggtcg agcgattcgg
     3061 gctgcggcgg cgctttcatg cggcagctgt ccgcaaagcg cttcatcgcg gctttctcct
     3121 gctcgtcgaa gcgcttgagg cgcgccatga ggtcatcgaa gtcgacctgg tggccgtcga
     3181 agtccgggcc gtcgacgcag gcgaacttca ccctgtcgcc gaccttgacc cggcagccgc
     3241 cgcacatgcc ggtgccgtc
//
LOCUS       DASUNT010000421         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_59893, whole genome shotgun sequence.
ACCESSION   DASUNT010000421 DASUNT010000000
VERSION     DASUNT010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59893"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1230)
                     /locus_tag="VFX94_08555"
     CDS             complement(52..1230)
                     /locus_tag="VFX94_08555"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5768270.1"
                     /translation="MRELPGAVRPLAIPAAILAIAAIAHLVGPPMPESLAGLFILGPY
                     VLLAIAVALAWGFNRGRAFVIAASLLCAFAAQQLYPGEVVYTALVCLVPFNALLALVL
                     PERGVRYRAAYVWVLLLVCEGLLIYGISRMEVALLDNMLLRSPPTPLAGRLFFAAAFA
                     AAVWRAWPEHTPLQVGNAGALVAFYIAAEWVGSAHVYASFMAAAGAILIVALVQESHR
                     LAFRDPLTGLPGRRALEERLSSLDGSYVISMVDVDHFKQFNDTHGHDIGDQVLKLVGA
                     RLADVGGGGTAFRYGGEEFCVVFPDAELEDVLLQLEAIRVSIERYRMAVRAPDRPKEL
                     EDGAKRRGEGVADKHLSVTVSIGACSPSSRLRTPAQVIKAADEALYRAKQGGRNRVST
                     "
     gene            complement(1297..>2345)
                     /locus_tag="VFX94_08560"
     CDS             complement(1297..>2345)
                     /locus_tag="VFX94_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX5768271.1"
                     /translation="IIKLIRASEDQAEAKEKLREKFRFTEKQAEDIVNLRLGQLTRLD
                     GVALNDERKALEAERKGLKDILGDEKELKKLVVAELVEDSKKFGDERRTLIKTAERAQ
                     VERTVVEEPVSVILSRKGWIRARTGHGLDVSQLSFKDGDALLQTLECKTTDPVTVLAA
                     SGKTFTVAAADIPSGRGDGAHVNTLANSGSDDIVWMGSGSPEQRLLMNSSAGLGFLCK
                     LGDLATKTRQGKDFLKVEEQARANAPLLITEGSSYLAALSSDARLLLFPLEEIPERPN
                     GGVGVQLLALPEKETLASVAASDGSSLVVSGIKRKNRAVETLDEKQLTLHIGKRAQRG
                     RLADVGLRPDRLGS"
BASE COUNT          393 a          791 c          770 g          391 t
ORIGIN      
        1 gttttcacgt acgtaaaaac gaattgcgac cctgaccccg aattcggagc atcatgtgct
       61 gaccctgttg cggccgccct gcttggcgcg gtagagggct tcgtcggcgg ccttgatgac
      121 ttgcgcgggg gtgcgcaagc gagaactcgg gctgcaggcg ccgatcgaga cggtgaccga
      181 caggtgcttg tcggcaacgc cctcgcccct gcgcttcgcg ccgtcctcga gctccttcgg
      241 acggtcgggg gcgcgcaccg ccatgcggta gcgctcgatc gagacgcgga tcgcctcgag
      301 ttgcagcaag acgtcctcga gctcggcgtc gggaaagacg acgcagaatt cctcgccccc
      361 gtagcgaaag gcggtgcccc cgccgccgac gtcggcgagc cgcgcgccca ccagcttcag
      421 gacctggtcg ccgatgtcgt ggccgtgggt gtcgttgaat tgcttgaagt ggtcgacgtc
      481 gaccatcgag atgacgtagc tgccgtcgag gctgctcagg cgctcttcca gcgcgcggcg
      541 gccgggaagc ccggtcagcg ggtcgcggaa cgcaagccgg tgggattcct gcaccagcgc
      601 gacgatcagg atcgcccccg cggccgccat gaacgaggca tacacgtgcg ccgagccgac
      661 ccattccgcg gcgatgtaga acgcgacgag cgcgcccgcg ttgcccacct gcagcggcgt
      721 gtgctcgggc cacgcgcgcc acaccgccgc ggcgaacgcg gcggcgaaga agagccggcc
      781 ggcgagcggc gtcggcggcg agcgcagcag catgttgtcg agcagcgcaa cttccatcct
      841 ggagataccg tagatcagca acccctcgca gacgagaagg agaacccaca cgtacgccgc
      901 gcggtagcgc acgccgcgct cgggcaggac cagcgcgaga agcgcgttga acggcacgag
      961 gcagacgagc gccgtgtaca ccacctcgcc cggatagagc tgctgcgcgg cgaacgcgca
     1021 gagcagcgac gccgcgatga cgaatgcgcg gccgcggttg aacccccacg cgagcgccac
     1081 cgcaatggcg agcaagacat acggcccgag gatgaaaagg ccggcgagcg attccggcat
     1141 cggcggcccc acgagatggg cgatggccgc gatcgcgaga attgccgccg ggattgccaa
     1201 cggccggacg gcgccgggaa gctcgcgcat cggcaaatcg taacggcttt tgcccggatt
     1261 ccctgtgacg acaggacagg aaacgtgcgg ttattctcag ctgccgagac gatcggggcg
     1321 caggccgacg tcggcgagcc tgccgcgctg tgcacgtttc ccgatatgca gcgtgagctg
     1381 tttctcgtcc agcgtctcca cggcgcgatt cttgcgtttg attccggaca ccacgagcga
     1441 gcttccgtcg ctcgccgcca ccgaagcgag cgtttccttt tccggcagcg cgaggagctg
     1501 tacgcccact cccccgttcg gccgctcggg aatctcctcg agcgggaaga gaagcaggcg
     1561 agcatccgac gacagggcag cgagataaga agacccctcg gtgatcaaca gcggggcatt
     1621 tgccctcgct tgctcttcca ccttcaggaa gtccttgccc tggcgggtct tggtcgcgag
     1681 atcgccgagc ttgcagagaa accccaggcc cgcggagcta ttcatcagca gtcgctgttc
     1741 cgggctgccc gagcccatcc atacgatgtc gtcggagccc gagttcgcca gcgtgttgac
     1801 gtgcgcgccg tcgccccgcc ccgaagggat gtcggcggcc gcaacggtga aggtcttgcc
     1861 ggaggcggcg agcacggtga ccgggtcggt ggtcttgcac tcgagcgtct gcagcagcgc
     1921 atcgccgtcc ttgaagctca actgcgacac gtcgagcccg tggccggtgc gcgcgcggat
     1981 ccagcccttg cgcgaaagga tcacgctcac cggctcctcg accacggtgc gctccacctg
     2041 cgcacgctcg gcggtcttga tgagcgtgcg cctctcgtct ccgaatttct tcgaatcctc
     2101 gaccaattcg gctacgacga gcttcttcag ctccttctcg tcgccgagga tgtccttcag
     2161 ccccttgcgc tcggcctcga gcgccttcct ctcgtcgttc agcgcgacgc cgtccagcct
     2221 ggtgagctgg ccgagccgga ggttcacgat gtcttcggcc tgcttttcgg tgaacctgaa
     2281 cttctcccga agtttctctt tcgcctcggc ttgatcttcg ctcgcgcgaa tgagcttgat
     2341 gatct
//
LOCUS       DASUNT010000422         3539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_411375, whole genome shotgun sequence.
ACCESSION   DASUNT010000422 DASUNT010000000
VERSION     DASUNT010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411375"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..147
                     /locus_tag="VFX94_08565"
     CDS             1..147
                     /locus_tag="VFX94_08565"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX5768272.1"
                     /translation="MEHPAIMTHASLPEDVRAGLGIDEGLVRLSVGIEAVDDLIAELK
                     YALE"
     gene            149..1096
                     /locus_tag="VFX94_08570"
     CDS             149..1096
                     /locus_tag="VFX94_08570"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX5768273.1"
                     /translation="MIGVVGALLALALGIAGVLHFMIRMPGTSHAGALPPLTGEARAL
                     SLRLRLHVAALAAAERNTDLETAARYIEQAFRAQGAAVQVQQFPSGGRTVRNIEAGKG
                     AIVVGAHYDTVPGSPGADDNASGIAVLLELAAHLKNESLPVRFVAFANEEPPYSFGPE
                     MGSWAWAKRARERGESIRAMYSLEMLGYYRDTPGSQHYPAPLGLFYPDRANFIAFVGD
                     LGARRLVRRSIDAFRKHAQFPSEGVAAPGFIPGVTWSDHWSFRQHGYPAMMITDTAFY
                     RYPHYHLPSDTPDKLDYERMARVTLGLAAMLRAISRQDE"
     gene            1104..1928
                     /locus_tag="VFX94_08575"
     CDS             1104..1928
                     /locus_tag="VFX94_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5768274.1"
                     /translation="MNDTIVLGELRVNRLGFGAMRVCGPSIWGPPKDRRRAHEVLRRA
                     IELGVNFFDTADSYGPYVDEELIAEALHPYPKDLVIATKGGIVRPSAARWDEDARPEH
                     LKRAIDGSLRRLKLERIDLYQLHAPDPRVPFMDSVGALADAQRAGKIRYIGLSNVDVT
                     RLEQARTLVNVVSVQNEYNLGNRESEPVLERCEKLGIAFLPWYPLGAGAVLRSARVKD
                     MAKKLQATPAQVAIAWLLARSPVMLPIPGTAAIKHLEENMRAAELRLAAEDVRALS"
     gene            1962..2918
                     /locus_tag="VFX94_08580"
     CDS             1962..2918
                     /locus_tag="VFX94_08580"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5768275.1"
                     /translation="MTRWIAVVACVALAVPAAAQEPYPSKPIRIIVGYSAGGGNDIIV
                     RVMQPELAKGLGQPVVVENRPGAQSIVAAELVAKAPADGYTLLMGPSGPMTINPATYS
                     KLPYHPQRDFAPVSMICSFPLLVVVSSSSAVKNIQDLVAYAKANPGKSNYASSAGIFQ
                     VASELFKQKTGTQFEMIPYKGSGESIQAVIAGQVTMTIVDPAPAAGPLKAGSIRGLAV
                     TSAQRHPNWPDVPTMAEAGVPDMEVPVWTAFFAPAKTPPAIVSRLQKEVARAVQTVEV
                     KERFATMGLVPVGSTEEELARVVARDIQKYTAVAKAANIKND"
     gene            complement(2942..>3539)
                     /locus_tag="VFX94_08585"
     CDS             complement(2942..>3539)
                     /locus_tag="VFX94_08585"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX5768276.1"
                     /translation="HSTFGTSDQPRAALISARKRCVAASSTDCAPALIALGAKVKLVS
                     SQGEREVPLEALYNNDGIDYLKRRPEEILTEIQVGNNLGSTYWKLRRRGAFDFPVLGV
                     AAAKTQEGVRIALGAVASRPSLVDKANAFLQGRELTDEVIDEAAKLVASRAKPMDNAD
                     LDLYWRKDVAAEFARYALRELRGDDMRETRLRVARQLV"
BASE COUNT          608 a         1194 c         1163 g          574 t
ORIGIN      
        1 atcgagcacc cggcgatcat gacgcatgcc tcgctgccgg aggacgtgcg cgcggggctg
       61 ggcatcgacg agggcctggt acggctctcg gttggcatcg aggccgtgga cgacctcatc
      121 gccgagctga aatacgcgct cgagtagggt gatcggcgtt gtcggcgcgc tccttgctct
      181 cgcgctcgga atagcggggg ttctccattt catgattcgt atgcccggca cctcacacgc
      241 cggtgcgctg ccgccgctca cgggagaggc gcgagcgctc tctttgaggt tgcgcctcca
      301 tgtggccgcg ctcgccgcgg ctgaacggaa caccgacctc gagactgccg cgcgctacat
      361 cgagcaggca ttccgcgcgc aaggcgccgc cgttcaggtt cagcagttcc cgagcggcgg
      421 gcgcacggtg cgcaacatcg aggccggcaa gggcgccatc gtggtcggcg cgcactacga
      481 caccgtaccg ggctcaccgg gggccgacga caatgcctcg ggcattgcgg tgctgctcga
      541 actggccgcc cacctcaaga acgaatcact tccggtccgg ttcgtagctt tcgcgaatga
      601 agagccgccc tactccttcg gcccggagat gggaagctgg gcctgggcaa agcgcgcgcg
      661 cgagcgcggc gagtcgattc gcgccatgta ctcgctggaa atgctcggct actaccgcga
      721 cacgccgggc agccagcact accccgcgcc gcttggcctg ttctatcccg accgcgcgaa
      781 cttcattgcc ttcgtgggcg acctcggcgc gcgccgtctc gtgcgccgct ccatcgacgc
      841 tttccgcaag cacgcgcagt ttccttcaga aggcgtcgcg gcgcccggct tcatcccggg
      901 cgttacgtgg tccgaccact ggtctttccg ccagcacggc tatcccgcaa tgatgatcac
      961 cgacaccgcg ttctaccgtt atccgcatta ccatctgccg agcgatacgc cggacaagct
     1021 cgattacgag cgcatggcgc gcgtcacgct cgggctcgcc gccatgctga gggcgatctc
     1081 gcggcaagat gaatgacacc aagatgaatg acaccatcgt gttgggtgaa ttgcgcgtaa
     1141 accggctcgg cttcggcgca atgcgcgtgt gcgggccgag catctggggc ccgcccaagg
     1201 accgcaggcg tgcgcacgag gtcttgcgcc gcgccatcga gctcggcgtg aatttcttcg
     1261 ataccgccga ctcgtacgga ccctatgtcg acgaggagct gattgccgag gcgctccatc
     1321 cctaccccaa ggacctcgtc atcgccacga aaggcggcat cgtgcggccg agtgctgcgc
     1381 gctgggacga ggacgccagg cccgagcacc tgaagcgcgc gatcgacggc agcctcaggc
     1441 gcttgaagct cgagcgcatc gacctctacc agctgcatgc gccggatccg cgggtgccgt
     1501 tcatggattc ggtcggagcg ctggccgacg cgcagcgcgc cggcaagatc cgctacatcg
     1561 ggttgtcgaa cgtcgacgtc acccgactgg agcaggcacg cacgctggta aacgtcgtct
     1621 cggtgcagaa cgaatacaac cttggcaacc gggaatcgga gcccgtgctc gagcgctgcg
     1681 aaaagcttgg tatcgcgttc ctgccgtggt acccgctcgg tgcgggggcg gtactgcgat
     1741 cagcccgggt gaaagacatg gcgaaaaagc tgcaagccac gccggcgcaa gtggcgatcg
     1801 cctggctgct tgccaggtcg ccggtcatgc tgccgatccc gggcaccgcc gcgatcaagc
     1861 acctggaaga aaatatgcgc gccgcggaac tgcggctcgc cgcggaggac gtgcgggcgc
     1921 tttcgtagtt aaactggcgc ccgacgaagg aggaggacgg aatgactcgt tggatcgcag
     1981 ttgtcgcgtg cgtcgcgctt gccgtgccgg cggcggcgca ggagccgtat ccgagcaagc
     2041 ctatccgcat catcgtcggc tactccgccg gcggaggcaa cgacatcatc gtgcgggtga
     2101 tgcaacccga gctcgccaag gggctcggcc agccggtcgt ggtggagaac cgtccgggcg
     2161 cgcagtcgat cgtcgccgct gagctcgtcg ccaaggcgcc ggccgacggc tacaccctcc
     2221 tcatgggccc gagcggcccg atgaccatca acccggcgac gtactccaag ctcccctatc
     2281 acccgcagcg ggatttcgcg cccgtctcga tgatctgctc gttcccgctg ctcgtggtgg
     2341 tgagctcgag ctccgcggtg aagaacatcc aggatctcgt cgcctacgcc aaggcgaacc
     2401 ccggcaagtc gaactacgcg tcgagcgcgg gcatcttcca ggtggcgagc gagctcttca
     2461 agcagaagac cggcacgcag ttcgagatga tcccctacaa gggaagcgga gagtcgatcc
     2521 aggcggtcat cgccgggcag gtgaccatga ccatcgtcga cccggcgccg gccgccgggc
     2581 cgctcaaggc gggctcgatc cgcggcctcg ccgtcacttc ggcgcagcgc catccgaact
     2641 ggcccgacgt gccaaccatg gccgaggcgg gcgtgcccga catggaagtc cccgtgtgga
     2701 ccgccttttt cgcgccggcg aagaccccgc cggcgatcgt ctcgcgcctg cagaaagaag
     2761 tggcgcgcgc ggtccagacc gtcgaagtaa aggagcgctt cgccaccatg gggctcgtcc
     2821 cggtcggctc gaccgaagaa gagctggcgc gcgtagtcgc tcgcgacatc cagaagtaca
     2881 ccgcggtcgc aaaagccgcc aacatcaaga acgactagaa aagggttagg acgcgctacg
     2941 gtcagaccag ctgccgcgcg acgcgcagcc gagtctcgcg catgtcgtcc ccgcgcagct
     3001 cgcgcagcgc ataacgtgca aactcggccg ctacgtcttt gcgccagtag agatccaggt
     3061 ccgcgttgtc catcggcttc gcgcgcgagg cgacgagctt cgccgcctcg tcgatcacct
     3121 catcggtgag ctccctgccc tgcaggaagg cattcgcttt gtcgacgagg gacggccggg
     3181 aagcgaccgc cccgagcgcg atccggacgc cttcttgagt cttggccgcc gcgaccccga
     3241 gcaccgggaa gtcgaacgcg ccgcggcggc gcagcttcca gtacgtactg cccaggttat
     3301 tgcctacttg aatctcggtg agaatctcct ccggccggcg cttgaggtaa tcgatgccgt
     3361 cgttgttgta gagcgcctcg agcggcactt cgcgctcacc ctggctggaa accagcttaa
     3421 ccttggcgcc gagggctatc agcgcgggcg cgcaatcggt cgacgaagcg gcgacgcagc
     3481 gcttgcgcgc cgagatcagg gcggcgcgtg gctggtccga ggtcccgaag gtgctgtgg
//
LOCUS       DASUNT010000423         4498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_673634, whole genome shotgun sequence.
ACCESSION   DASUNT010000423 DASUNT010000000
VERSION     DASUNT010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673634"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /gene="dctP"
                     /locus_tag="VFX94_08590"
     CDS             <1..672
                     /gene="dctP"
                     /locus_tag="VFX94_08590"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX5768277.1"
                     /translation="SRIHWKHFHKLGEYKGVKLLGVFTHGPGQMFTKKPVAHINDIKG
                     LKIRTGGGVAEQVANALGASAFVKPAPESYELLKSGVADGVFFPLESIVSFKLETVLE
                     QATLLPGGMYSSAFGFFMNEGKWNKLPKQDQAAIEKLGYEHVARTAGMAWDAGDKKGM
                     DVLRASGVKVISADKALQAEVQKRSAPLIEDWVKKASAKGVDAKAILEEFRAELKKVA
                     ASEKK"
     gene            751..1152
                     /locus_tag="VFX94_08595"
     CDS             751..1152
                     /locus_tag="VFX94_08595"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX5768278.1"
                     /translation="MMTLTFVDVVGRYVFNRPLRGGFEITELLLLVLIFAGLPLVSHA
                     DEHVTMDFIDRVAGPRATAIVKRAVHVLVAALFFLLTWLVWLRADRVSSYGDATDVLR
                     IAYGPFVYFMAVMIALTGLLHLYKAFVWPKG"
     gene            1137..2435
                     /locus_tag="VFX94_08600"
     CDS             1137..2435
                     /locus_tag="VFX94_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004567252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX5768279.1"
                     /translation="MAEGLIGLAAMLLLAFLRIPIALAMGIVGIVGYAYVRDWNWTVA
                     FAIAQTKIYETGRNYTLSVVPLFILMGNFVTRAGMSQELFRAAYGFIGHLRGGLAMAT
                     IIACAGFGAICGSSIATAATMAKVAYPSMKRFGYSDRLATGAIASGGTLGIMIPPSTI
                     MVIYGVFTETNIGMLFAAGVLPGILGAALRCAAVRYVTWRDPSAGPPGERSSWRERWR
                     AIRPIWAVAFLFLFVIGGIYGGLFTATEGAGMGAFGAMVFALWRRALDWKTLYAALVE
                     SARTTAMLFMILIGALMFADFVNITTMPSDLKAFVTQFQLHPIAVVAAICIIYVLLGT
                     AMEELSMVLLTLPVFFPVIVHLGYDPVWFGILIVVVVEIGLISPPVGMNLFVLKTLLP
                     SVSTGTVFRGVMPFMLADCIRFALLIAFPAISLYLPSLMK"
     gene            2432..3112
                     /locus_tag="VFX94_08605"
     CDS             2432..3112
                     /locus_tag="VFX94_08605"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF005484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEX5768280.1"
                     /translation="MKRQVLAVACRILAAEAHESGLAGQVTARADKPGTWWTLEFGYG
                     FDEATPGRMVLVDEDLKPLSGGRPNPSVRVHVWIYRRRPEVQAIVHTHAPHASALAST
                     GKALKTIHMDSAMLHGAAHLPEWPGVPLGDDEGRIISDALGDAKTILLANHGLLATGA
                     YVEEAAYLAVFFERAARMQLRAMAAGGYQEVKPELAEEARDFLLQPSIVRATFAYWAR
                     TLSARNAR"
     gene            complement(3073..4026)
                     /locus_tag="VFX94_08610"
     CDS             complement(3073..4026)
                     /locus_tag="VFX94_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HEX5768281.1"
                     /translation="MALIDLAQIAGGLIALYFGAGWLVGAATAVARGLGVSPLLVGLT
                     VVALGTSLPELVVGVRAALGGHPGISLGNVVGANAINISLILGAAALIRPLAVELRVL
                     KWDLPAMLAAAILMTAMAANGEVGRFEAAVLLACMAAYVGLNIVLARRERSTRALERF
                     AAGVEPTATPAWREAARGVAGLAALLVGAELLIAGALALAAALGASEGIVGLTIVAAG
                     TTLPELLTATVAAARGQGDIAFGNVIGSNIANALAVVGASAAIRPLPAGDLGWPAIGG
                     MLALSLLSVPILWRGFRVTRAEGVVLIAAYLAFLALNVRAQ"
     gene            complement(4013..>4498)
                     /locus_tag="VFX94_08615"
     CDS             complement(4013..>4498)
                     /locus_tag="VFX94_08615"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX5768282.1"
                     /translation="GLKSLVFEGPARVFDSEEACADVVKQRKYKEGEVLVIRYEGPKG
                     GPGMREMLGVTALIYGQGMGETVALVTDGRFSGATRGMMIGYVSPEAAAGGPLALLRD
                     GDRIRIDASAGSIEAFADLKKRKARPPKRAVSGVLEKYAALVGPANLGAVTHRGPRGV
                     D"
BASE COUNT          730 a         1554 c         1456 g          758 t
ORIGIN      
        1 tcgcgcatcc actggaagca ctttcacaag ctcggcgagt acaagggcgt caagctgctc
       61 ggcgtcttca cgcacggccc cggccaaatg ttcacgaaga agccggtcgc tcacatcaac
      121 gacatcaagg gtctgaagat ccgcaccggc ggcggcgtgg ccgagcaggt ggccaatgcc
      181 ctcggcgctt ccgcgttcgt gaagcccgcg cccgagtcat acgagctgct gaagtcgggg
      241 gtcgccgacg gcgtgttctt tccgctcgag tcgatcgtct cgttcaagct cgagacggtg
      301 ctcgagcagg cgacgctctt accgggcggc atgtacagct cggcgttcgg gttcttcatg
      361 aacgaaggca agtggaacaa gctgccgaag caggaccagg ccgcgatcga gaagctcggc
      421 tacgagcacg tcgcgcgcac cgccggcatg gcctgggacg cgggcgacaa gaaaggcatg
      481 gacgtgctga gagcctcggg cgtgaaggtg atcagcgcgg acaaggcgct gcaagccgaa
      541 gtgcagaagc gctcggcccc gctgatcgaa gactgggtca agaaagcgag cgccaagggc
      601 gtcgacgcca aggcgatcct ggaggagttc cgcgccgagc tgaagaaagt cgcggcctca
      661 gagaagaagt agcttacgaa gcaagagtct ttttgggaga ggcgggccga cgcggtcctg
      721 ggcgtcgccg cctcgatcat ccttttctgc atgatgacgc tcacgttcgt cgacgtggtg
      781 gggcgctacg tcttcaaccg gccgttgcgc ggcggattcg agatcaccga gctcctgctc
      841 ctcgtgctga tcttcgccgg actgccgctc gtgtcgcacg ccgacgagca cgtcaccatg
      901 gacttcatcg accgcgtcgc ggggccgcgc gcgaccgcga tcgtgaagcg cgcggtgcat
      961 gtcctcgtcg cggcgctatt cttcctcctc acctggctcg tgtggctgcg cgccgaccgc
     1021 gtctcctcgt acggcgacgc caccgacgtg ctgcgcatcg cctacggacc gttcgtgtac
     1081 ttcatggcgg tgatgatcgc cctcaccggg ctgctccatc tctacaaggc gttcgtatgg
     1141 ccgaagggct gatcggcctc gcggcgatgc tcctgctcgc gttcctgcgc atcccgatcg
     1201 cgctcgccat gggcatcgtg ggcatcgtcg gctacgccta cgtgcgcgac tggaactgga
     1261 ccgtcgcctt cgccatcgcg cagaccaaga tctacgagac cgggcgcaac tacacgttgt
     1321 cggtggtgcc gcttttcatc ctgatgggca acttcgtcac gcgcgccggc atgtcgcagg
     1381 agctcttccg cgccgcctac ggtttcatcg gccacctgcg cggcggcctt gccatggcga
     1441 cgatcatcgc ctgcgccggg ttcggcgcca tctgcggctc ctccatcgcc accgcggcga
     1501 ccatggcgaa ggtggcctac ccttcgatga agcgcttcgg gtactccgac cgcctcgcca
     1561 ccggcgccat cgcctcgggc ggcaccctcg gcatcatgat cccgccctcg accatcatgg
     1621 tgatctacgg tgtgtttacc gagaccaaca tcgggatgct cttcgccgcc ggcgtgctgc
     1681 cgggaatcct cggcgccgcg ctgcggtgcg ccgccgtgcg ctacgtcacc tggcgcgacc
     1741 cgagcgccgg gccgccgggc gagcgctcga gctggcgcga gcgctggcgg gcgatccggc
     1801 cgatctgggc ggtcgcgttc ctcttcctct tcgtgatcgg tggaatttac ggaggcctct
     1861 tcaccgcgac cgagggcgcc ggcatgggcg ccttcggcgc gatggtgttc gcgctctggc
     1921 ggcgcgcgct cgactggaag acgctgtacg cggcgctcgt cgagagcgcg cgcaccaccg
     1981 cgatgctctt catgatcctg atcggcgcgc tgatgttcgc cgacttcgtc aacatcacga
     2041 ccatgccttc ggacctcaag gcgttcgtga cgcagttcca gctccacccg atcgccgtcg
     2101 tagcggcgat ctgcatcatc tacgtgctgc tcggcacggc gatggaagag ctctcgatgg
     2161 tgctgctcac gctgccggtg ttcttcccgg tcatcgtgca cctcggctac gacccggtct
     2221 ggttcggcat cctgatcgta gtggtggtcg agatcggcct catcagcccg ccggtcggca
     2281 tgaacctctt cgtcctgaag acgctgcttc ccagcgtgtc caccggcacc gtgttccgcg
     2341 gcgtgatgcc gttcatgctc gccgactgca ttcgtttcgc gctgctgatc gcctttccgg
     2401 cgatttcgct ctacctgccc agcctgatga aatgaagcgg caagttctcg ccgtggcttg
     2461 ccgaatcctc gccgcggaag cacacgagtc gggtcttgcc ggacaggtca ctgcaagagc
     2521 agacaaaccg ggcacctggt ggacactgga attcggctac ggcttcgacg aggcgactcc
     2581 cggacgcatg gtgctcgtcg acgaggacct caagccgctt tccggcggcc ggccgaatcc
     2641 aagcgtgcgc gttcatgtat ggatctaccg caggcggccc gaggtgcaag caatagtcca
     2701 cacgcacgct ccgcacgctt cggctcttgc aagtaccggg aaggctttga agacgatcca
     2761 tatggacagc gcgatgctgc acggcgcagc gcacctgccc gaatggcccg gagtcccgct
     2821 gggcgacgat gaaggacgca tcatctcgga tgcgctgggc gacgcaaaga ccatcttgct
     2881 tgccaaccac ggcctgctcg ctacgggcgc ttatgtggaa gaggcagctt acctcgcggt
     2941 cttcttcgag cgtgcggcgc gcatgcagct tcgcgcgatg gcggccggcg gctaccagga
     3001 agtgaagccc gagctcgccg aggaagcgcg cgacttcctg ctgcagccct cgatcgtcag
     3061 ggcgactttc gcttactggg cacgaacgtt gagcgccagg aacgcgagat aggctgcgat
     3121 cagcaccacg ccttcggcgc gggtgacgcg aaagccgcgc cacaggatgg gcaccgagag
     3181 cagcgagagc gcgagcatcc cgccgatggc cggccatccc aggtcgcccg ccggcagcgg
     3241 acgaatcgcc gctgacgccc cgaccactgc gagcgcattg gcgatgttcg agccaatcac
     3301 atttccgaat gcgatatcgc cttggccgcg cgctgccgcg acggtggcgg tgagaagctc
     3361 gggaagcgtc gtgccggcgg cgacgatcgt gaggccgacg atgccttccg atgcgccgag
     3421 ggcggcggcg agcgcgagcg cgcccgcgat gagcaactcg gccccgacaa gcagcgccgc
     3481 cagtccggcc actccgcggg cggcttcgcg ccaggccggt gtcgcggtcg gctcgacgcc
     3541 ggctgcgaaa cgctcgagcg cccgcgtgct gcgctcgcgc cgcgcgagca cgatattcag
     3601 cccgacatac gccgccatgc acgcgagcag cacggcagcc tcgaaccggc cgacttcgcc
     3661 gttcgccgcc atcgcggtca tgagaattgc ggccgccagc atcgcgggca agtcccattt
     3721 gagcacgcgc agctcgaccg ccagcggccg gatcagcgcc gccgctccga gaatgagcga
     3781 gatgttgatc gcgttcgcgc cgacgacgtt gccgagcgag atgcccgggt ggccgccgag
     3841 cgccgcgcgc acgccgacga cgagctccgg caggctcgtg ccgagagcaa cgaccgtcag
     3901 cccgacgagc agcggggaga cgccgagccc gcgcgccacc gcggtcgccg cgcccaccag
     3961 ccagccggcg ccaaaataga gcgcgatcag tccgcccgcg atctgggcga ggtcaatcaa
     4021 cgccacgagg accgcggtgc gtcaccgcac cgaggttggc cgggccgacg agcgcggcgt
     4081 acttctcgag cactcccgag accgcgcgct tcggcggcct ggccttgcgt ttcttcaagt
     4141 ctgcaaacgc ttcgatactg cctgccgatg catcgatgcg gattcgatcg ccatcgcgca
     4201 gcagcgcgag cggcccgccg gccgcggcct cgggggacac gtagccgatc atcattccgc
     4261 gggtcgcgcc cgagaaacgc ccatcggtga cgagcgcaac cgtctcgccc atcccctggc
     4321 cgtaaatcag cgcggtgaca cccagcatct cgcgcatccc ggggccgcct ttcggtcctt
     4381 cgtagcggat gaccagcacc tcgccttcct tgtatttcct ttgtttcacg acgtcggcgc
     4441 aggcctcctc cgagtcgaac acgcgtgccg gcccctcgaa cacgagcgac ttcaaccc
//
LOCUS       DASUNT010000424         3854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1111957, whole genome shotgun sequence.
ACCESSION   DASUNT010000424 DASUNT010000000
VERSION     DASUNT010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111957"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VFX94_08620"
     CDS             <1..1149
                     /locus_tag="VFX94_08620"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX5768283.1"
                     /translation="TMNLDSCGSCHAQPATGGTSPPTNPQAAFANAARPSQYPAVQSF
                     IRPDGPVREARFVRNPDGTPDGGVHALFTISGREGADGGCSLQQPDFAGELERGNVIF
                     RIPTPVFGAGLIEQIPDSAILENMAANTGQKSALGIAGRPNVALSGRTVSGSPNRNGN
                     DGTIARLGWKAQNQSLLVFSGEAYNVEMGITSELFQSERHQDEACQYANVPNDMQNFD
                     ARDALSGTTAIQNFANFQRFLAPPKPSPDTPGGAASIKAGRALFASVGCDLCHTPTLH
                     TSTRSAVAALRNREANLYSDLLVHDMGEGLADGVSQGSAGPREFRTAPLWGLGQRIFF
                     LHDGRTSDLKVAIREHASHGSEANGVIRKYNHLSESEKQHLLNFLRSL"
     gene            complement(1146..1904)
                     /locus_tag="VFX94_08625"
     CDS             complement(1146..1904)
                     /locus_tag="VFX94_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5768284.1"
                     /translation="MLVAGCGPLSPLPAGEVGFGVLGDAPYSEEEVERLERLIDEINA
                     QALEFVVHLGDIGSSAQACGDAWLLERKKQFARIRHRFILLPGDNEWSNCKEPLSRLR
                     RWREIFCEKPGEFCEHQRWEAGGWVFVALNVPGHDNNLRHSEFTPRMRAVLAWLDAAA
                     TAAEERAGLVVLMQANPFLTFPRDGFAELRQALERLAAKSPGRVVLIHGDTHVYRQDE
                     PLPGLLRIEVWGSPIVSWMRASVSPQALRVDAVR"
     gene            complement(1910..3235)
                     /locus_tag="VFX94_08630"
     CDS             complement(1910..3235)
                     /locus_tag="VFX94_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX5768285.1"
                     /translation="MREGSLEAPTRHALAWTDPEFWDQAKAEKELGRIFDICHGCRRC
                     VSLCTTFPTLFDLVDESPTMEVDGVGKADYWKVVDQCYLCDLCYMTKCPYVPPHEWNV
                     DFPHVMLRAKAIKFRQGVKLRDRLLSSTDALGKLAAIPVVAQTANAVNRNRISRGILQ
                     SLLGVHSERELPPYAPRKFRSGAAASAPFPVKDGANTPGKVAVFATCYANYNEPVLGH
                     DLLAILAHNEIPYVMVESEVCCGMPKLELGDLDGVDALKNKNMTVLARYAREGYAILA
                     PVPSCALMFKQELPLMYPEDPEAIAVRDAMFDPFEYFVLRRKDGLLKTSFVNPLGKVA
                     YHIPCHSRVQNMGQKTREMLESIPGTEVTTVERCSGHDGTWGVKSEYFENSMKIGRPV
                     FRQMAEPGPKYVSSDCPIAARHILQGMEGAAKGVVRAHPLTLLRSAYGI"
     gene            complement(3244..3666)
                     /locus_tag="VFX94_08635"
     CDS             complement(3244..3666)
                     /locus_tag="VFX94_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX5768286.1"
                     /translation="MKALKGSKTHDNLKAAFAGESQANRRYLYFAAKADVEGQNDVSA
                     LFRSTAEGETGHAHGHLEYLEQVGDPATNLPIGRTRENLKAAVAGETHEYTDMYPGMA
                     KQARDEGFGEIADWFETLAKAERSHANRFQKALDSLSD"
BASE COUNT          635 a         1294 c         1265 g          660 t
ORIGIN      
        1 acgatgaacc tcgacagctg cgggagctgc cacgcgcagc ctgcgaccgg cggcacgagc
       61 ccgccaacga atccgcaagc cgcgttcgcc aacgcggcgc gtccgtcgca gtatcccgcg
      121 gtgcagagct ttatccggcc agacgggccg gtgcgcgaag cgcgcttcgt gcgcaatccc
      181 gacggcacgc ccgacggcgg cgtgcacgcg ctcttcacca tcagcggacg cgagggggcc
      241 gacggcggct gcagcctgca gcagccggac ttcgccggcg agctcgagcg cggcaacgtg
      301 atcttccgca ttccgacccc ggtatttggc gccgggctga tcgagcaaat cccggattcg
      361 gccatcctgg agaacatggc ggcgaacacg gggcagaaaa gcgcgctcgg gatcgctggc
      421 aggccgaacg tcgctctctc gggccggacc gtgtcggggt cgccgaaccg caacggcaac
      481 gatggcacca tcgcgcgcct gggctggaaa gcgcagaacc agtcgctgct cgtgttctcg
      541 ggcgaggcgt acaacgtcga gatgggcatc acgagcgagc tcttccagtc ggagcgccac
      601 caggacgagg catgccagta cgcgaacgtg cccaacgaca tgcagaattt cgacgcgcgc
      661 gatgcgctga gcggcaccac cgcgatccag aacttcgcca acttccagcg cttcctcgca
      721 ccgccaaagc cctcgccgga cacgccggga ggagcggcat cgatcaaggc ggggcgggcg
      781 ctcttcgcga gcgtgggctg cgacctttgc cataccccga cgcttcacac ttccacgcgc
      841 tccgcggtgg ctgcgctgcg caatcgcgag gcgaacttgt actccgatct cctggtgcac
      901 gacatgggag aagggctcgc cgacggcgtg agccagggca gcgctggccc gcgcgagttt
      961 cgcaccgcgc ccctgtgggg gctcgggcag cgcatcttct tcctgcacga cggccgcacg
     1021 tccgacctga aggtcgcgat ccgcgagcat gcgagccacg gctcggaagc gaacggcgtc
     1081 atccgcaagt acaaccactt gtcggaaagc gaaaagcagc acctgctcaa cttcctgcgc
     1141 tcgctttagc gcaccgcatc cacgcgcagc gcttgcggcg acaccgaagc gcgcatccag
     1201 ctcacgatgg gcgagcccca cacctcgatg cggagcagcc ccgggagcgg ctcgtcctga
     1261 cggtagacgt gagtgtcgcc gtggatcagg acgaccctgc cgggcgactt cgcggccagc
     1321 cgttcgagcg cctgcctgag ctccgcgaag ccgtcgcgcg ggaaagtgag gaacgggttc
     1381 gcctgcatca ggaccacgag gcccgcgcgc tcttccgcgg ccgtggccgc ggcatcgagc
     1441 caggcgagga ccgcccgcat gcgcggcgtg aactcggaat ggcgcaagtt gttgtcgtgg
     1501 cccgggacgt tgagggcgac gaagacccag ccgccggctt cccagcgctg gtgctcgcag
     1561 aattcgccgg gtttttcaca aaagatctcg cgccaccgcc gaagacgtga cagcggttcc
     1621 ttgcagttgc tccactcgtt atcgccgggc agcaggatga accgatggcg gatgcgcgcg
     1681 aactgcttct tgcgctcgag gagccaggcg tcgccgcagg cctgcgcgct cgagcctatg
     1741 tcgcccaggt gcacgacgaa ctcaagcgcc tgcgcgttga tctcgtcgat cagccgctcg
     1801 agccgctcga cctcttcctc gctataggga gcatcgccca ggacgccgaa ccccacctcg
     1861 ccggcaggca gcggggaaag cgggccgcag cccgcgacaa gcacgaaggc tagatgccgt
     1921 aggcgctgcg tagcagggta agcggatggg ccctgaccac gcccttcgcc gctccctcca
     1981 tgccctgcaa aatgtggcgc gcggcgatcg ggcagtcgga gctcacgtac ttcgggccgg
     2041 gctcggccat ctgcctgaac accgggcggc cgatcttcat cgagttttcg aagtactcgc
     2101 tcttcacgcc ccaggtgccg tcgtggccgg agcagcgttc caccgtggtc acctcggtgc
     2161 cgggaatcga ttccagcatc tcacgcgttt tctggcccat gttctgcacg cgcgagtggc
     2221 acgggatatg gtaggccacc ttgccgagcg ggttgacgaa gctggtcttc agtaatccgt
     2281 ccttcctgcg cagcacgaaa tattcgaacg ggtcgaacat cgcgtcgcgc accgcgatcg
     2341 cctccgggtc ctccggatac atgagcggca gctcctgctt gaacatgagc gcgcaggagg
     2401 gcaccggagc gaggatcgcg tagccttcgc gcgcgtagcg cgcgagcacg gtcatgttct
     2461 tgttcttgag cgcgtccacc ccgtcgagat cgcccagctc gagcttcggc atgccgcagc
     2521 acacctcgct ctcgaccatg acgtatggga tctcgttgtg cgccaggatc gcgagcaggt
     2581 cgtgtcccag gaccggctcg ttgtagttcg catagcaggt ggcgaagacg gccacctttc
     2641 ccggcgtgtt ggctccgtcc ttcaccggga aaggcgcgga agccgcggcg ccgctgcgga
     2701 acttcctcgg cgcgtacggc ggcagctcgc gctcgctgtg gaccccgagc agcgattgca
     2761 gaattccccg ggatatccgg tttcgattga ccgcattggc cgtctgcgcc accaccggaa
     2821 tcgcggcgag cttgccgagc gcgtcggtcg aggaaagcag gcggtcgcgc aacttgacgc
     2881 cctgccggaa cttgatcgcc ttcgcgcgca gcatgacgtg cggaaagtcc acgttccact
     2941 cgtgcggcgg gacgtacggg catttcgtca tgtagcagag gtcgcagagg tagcactggt
     3001 cgacgacctt ccagtaatcc gctttgccga ccccgtcgac ctccatcgtc ggcgattcgt
     3061 cgacgaggtc gaagagcgtg gggaacgtgg tgcacaggct gacgcagcgc cggcagccgt
     3121 ggcagatgtc gaagatgcgc ccgagctcct tctcggcctt cgcctggtcc cagaactcgg
     3181 ggtccgtcca ggcaagcgca tgccgcgtcg gagcttcgag cgatccttcc ctcatcgaat
     3241 cacttagtcg gaaagcgaat cgagcgcttt ctggaagcgg ttcgcgtggc tgcgctcggc
     3301 cttcgccagc gtctcgaacc agtcggcgat ctcgccgaaa ccttcgtcgc gcgcctgctt
     3361 cgccatgccc gggtacatgt cggtgtactc gtgggtctcg cccgcgaccg cggctttcag
     3421 gttctcgcgc gtacggccga tcgggaggtt ggtggccgga tcgccgacct gctcgaggta
     3481 ttcgaggtgg ccgtgcgcgt gcccggtctc gccttcggcc gtggagcgga agagcgccga
     3541 gacgtcgttt tggccttcga cgtccgcctt cgctgcgaaa taaagatagc gccggttcgc
     3601 ctgcgattcg ccggcgaagg cggccttgag gttgtcgtgg gtcttgctgc ctttgagcgc
     3661 tttcatggaa gtcctcctgt ggggaaaacg agcctacggc cgcgcgctca atagggcaaa
     3721 tttaatttgg ttatctggtt gatcgtctcc gtcaattact tgcgcgtgac ggcgtcacgc
     3781 acgtcgcgcc ccgcctcgtc gagctggcgg ccggcttttt ccgccgggcc ttcgcgttcg
     3841 cacgcggcga ggcc
//
LOCUS       DASUNT010000425         2226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_60133, whole genome shotgun sequence.
ACCESSION   DASUNT010000425 DASUNT010000000
VERSION     DASUNT010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60133"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VFX94_08640"
     CDS             complement(<1..862)
                     /locus_tag="VFX94_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5768287.1"
                     /translation="MNRLLEKLNPYPFERLRALIGGKPALINLSIGEPKHPTPRLIRD
                     ALAGALDGLATYPATAGTPELRAAISAWLGRRYGIPAPDPATQVLPVNGSREALFSFA
                     QALIDPRPGCKVVCPNPFYQIYEGAALLAGATVLYGEPESWAGVQLLYACSPANPSGR
                     VMGLEDWKRLFELSDRHGFVIASDECYSEIYFDSPPLGALEAACRLGRSDFRNLVVFS
                     SLSKRSNAPGMRSGFVAGDAALLKGFLLYRTYHGSAMSLAVQAASIAAWNDEQHVREN
                     RRLYAEKFSAV"
     gene            complement(837..1226)
                     /gene="queF"
                     /locus_tag="VFX94_08645"
     CDS             complement(837..1226)
                     /gene="queF"
                     /locus_tag="VFX94_08645"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX5768288.1"
                     /translation="MPSRPSKRLDTFPNPTPQRDYRIHMEIPEFTCLCPMSGQPDFAT
                     LVLDYVPDRLCVELKSLKLYVWSYRDEGAFHEALTNRILDDLVRATRPRYLKLEARFN
                     VRGGIYTSVIAEHRKKGWRGEPASRKA"
     gene            1274..>2226
                     /locus_tag="VFX94_08650"
     CDS             1274..>2226
                     /locus_tag="VFX94_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5768289.1"
                     /translation="MRLTQIKLSGFKSFVDPTAIHIPGQLVGVVGPNGCGKSNVIDAV
                     RWVLGESRASALRGDSMQDVIFNGSVNRKPLARASVELIFDNSQGRAAGQWSQYAEIS
                     VKRTLQRDAESSYYINGTHVRRRDVTDMFLGTGLGPRAYAIIEQGMISRVIEAKPEEL
                     RVFLEEAAGISRYKERRRETENRLADTRDNLARVTDIRAELGAQLEKLQAQARVATRY
                     KELQAELQLKQHLLWYLRRRDAAAERERHAQDIDKATHALESETAQLRNVEARVETAR
                     AAHYAAGDAMNAAQGALYAANAEVARFESELRHAEETRSRL"
BASE COUNT          412 a          721 c          766 g          327 t
ORIGIN      
        1 acacggcgct gaacttctcg gcatacaggc ggcggttctc gcgtacatgc tgctcgtcgt
       61 tccaggccgc gatcgaagcc gcctgcaccg cgaggctcat ggccgagccg tggtaggtgc
      121 ggtagaggag gaagcccttc agcagcgcgg catcgccggc gacgaagccc gaacgcatgc
      181 ctggcgcgtt ggagcgcttc gacaaactgg aaaaaacgac gaggttgcgg aagtcgctgc
      241 ggccgagccg gcaggcggct tccagcgcac cgagcggcgg ggaatcgaag taaatctcgg
      301 agtagcactc gtcggacgct atgacaaagc cgtgccggtc ggaaagctcg aagagccgct
      361 tccagtcctc gaggcccatc actcgtccgc tgggattggc gggcgagcag gcgtagagga
      421 gctgcacccc cgcccagctt tccggctcgc cgtagagcac agtcgccccc gcgagcaggg
      481 ccgcgccttc atagatctga tagaaaggat tcgggcagac caccttgcag ccgggccgag
      541 gatcgatgag cgcctgggca aaggaaaaaa gcgcctcacg cgacccgttg accggcagga
      601 cctgcgtggc cggatcgggc gccggaatgc cgtagcgacg accgagccag gccgagatcg
      661 cggcgcgcag ctcgggcgtg ccggcggtgg ccggataggt ggcaaggccg tcgagcgcgc
      721 ccgccagcgc atccctgatg agacgcgggg tcggatgctt cggctcgccg atcgagagat
      781 tgatgagcgc cggcttgccg ccgatcaggg cgcgcagcct ctcgaagggg taggggttaa
      841 gcttttcgag aagccggttc accccgccag ccctttttcc gatgctcggc gatgacgctc
      901 gtgtaaatgc cgccgcgaac gttgaaccgc gcttcgagct tcaggtagcg cggccgggtc
      961 gcccgcacga gatcgtccag gatgcggttg gtcagcgctt cgtgaaaggc gccttcgtcg
     1021 cgataggacc acacatagag cttgagcgat ttcagctcga cgcagagccg atcaggcacg
     1081 tagtcgagca cgagcgtggc gaaatccggc tggccgctca tcggacacag gcaggtgaac
     1141 tccgggatct ccatgtggat ccggtagtcg cgctgcggcg tcggattggg aaaagtgtcc
     1201 agccgcttgc tcggtcggga aggcaaatga aaaaccgggc ggagagaaag cgcggcgatg
     1261 ttatcatttt tgcgtgcgcc taactcaaat aaaactctcc ggattcaagt ccttcgtcga
     1321 tcccaccgcg atccacatcc ccggacagct cgtcggcgtg gtcggcccga atggctgcgg
     1381 caagtccaac gtcatcgatg cggtgcgctg ggtgctcggc gaatcgcgcg cctcggcgct
     1441 gcgcggggac tcgatgcagg acgtgatttt caacggctcg gtgaaccgca agccgctcgc
     1501 gcgcgcctcg gtcgagctca ttttcgacaa ctcgcagggc cgcgccgccg ggcagtggtc
     1561 gcagtacgcc gagatctccg taaagcgcac cctgcagcgc gacgccgagt cgagctacta
     1621 catcaacggc acgcacgtgc gccggcgcga tgtcaccgac atgttcctcg gcaccggcct
     1681 cggccctcgc gcctacgcga tcatcgagca gggcatgatc tcgcgcgtga tcgaggcgaa
     1741 gcccgaggag ctgcgcgtgt tcctcgagga ggcggcgggc atctcgcgct acaaggagcg
     1801 ccggcgcgag accgagaacc gcctcgccga cacgcgggac aacctggcgc gcgtgaccga
     1861 catccgcgcc gagctcggcg cccagctcga gaagctccag gcgcaggcga gggtggctac
     1921 gcgctacaag gagctccagg cggagctcca gctcaagcag caccttctct ggtatttgcg
     1981 gcggcgcgac gccgccgccg agcgcgagcg ccatgcgcag gacatcgaca aggccacgca
     2041 cgccctcgag tccgagacgg cgcagctgcg caacgttgaa gcgcgcgtgg agacggcgcg
     2101 cgcggcgcac tatgccgcgg gcgacgccat gaacgccgcc cagggcgcgc tctacgccgc
     2161 caatgcggag gtggcgcgct tcgaatccga gctgcggcac gccgaggaga cgcgcagccg
     2221 cctgga
//
LOCUS       DASUNT010000426         2562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_236285, whole genome shotgun sequence.
ACCESSION   DASUNT010000426 DASUNT010000000
VERSION     DASUNT010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236285"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFX94_08655"
     CDS             complement(<1..282)
                     /locus_tag="VFX94_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX5768290.1"
                     /translation="MRDFFNLYRHGFARMAVATPLVRVADPDYNRDATLELMREAARE
                     KAVLAVFPELGLSAYSCEDLFHQRALVDSAEDALAELLGKTRTLPLAALV"
     gene            325..504
                     /locus_tag="VFX94_08660"
     CDS             325..504
                     /locus_tag="VFX94_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768291.1"
                     /translation="MLLLRETVLTRAGTLEVELHDDLRYRLSWKGVVYDHKRRYDFRS
                     VEQLRYDFERDVEKA"
     gene            488..841
                     /locus_tag="VFX94_08665"
     CDS             488..841
                     /locus_tag="VFX94_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5768292.1"
                     /translation="MSRKLEIRIGAAADALDRFEAAWNRVREGRAQRPLEVLSFEDLP
                     LLTKTLSPARWTLLRDLREKGACSIYELAKRLGRDYKNVHTDVTQLAAIGLVERRADG
                     LAAVTWDLLRAELEL"
     gene            902..2236
                     /locus_tag="VFX94_08670"
     CDS             902..2236
                     /locus_tag="VFX94_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5768293.1"
                     /translation="MNRDLSATSALNLEPYWMPFTDNRRFKAKPKLVVAAKDMHFTTA
                     EGRQVLDGLATLWCVNAGHGRARIVEAIRRQAGELDFAASFSLGHPGAFRLAERVAAL
                     APSGLGHVFFTNSGSEAIDTAMKIAVGYHRLRGEGTRTRFVGRERSYHGVNIGGISLG
                     GVPANRKAYAGVLLAGVDHLRHTHLPENAFTKGQPARGAELADELERIVALHDASNIA
                     ALVVDPVSASGGVLIPPKGYLERLRELCTRHGILLVFDEVITGWGRLGTPFAAQHFGV
                     EPDLITFAKGITSGAVPLGGVIVKDSIYQTFTGAQGPGIEFFHGYTYSGHPLACAAGI
                     AALDTYEEEGLFKRGAALAKDFEDAIHSLKGLPNVVDCRNLGLIGAVELAQRPGAPYG
                     TRAFEVFEKCWDKGVFVRPVGEALAFCPPLIAQKKHLDLMFGVVGEVLKEIA"
     gene            complement(2238..>2562)
                     /locus_tag="VFX94_08675"
     CDS             complement(2238..>2562)
                     /locus_tag="VFX94_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5768294.1"
                     /translation="TAMADCARRANVDMRIVEEWTWNAELFDPECIALVRDTARRLGV
                     ASLDLPSQAGHDAYHVARVAPTAMMFSPCKDGITHNNKEHADLERTAPAVNVLANVVL
                     QRANR"
BASE COUNT          411 a          842 c          884 g          425 t
ORIGIN      
        1 gacgagcgct gccaatggaa gcgttctcgt cttcccgagc aattcggcga gcgcatcttc
       61 ggccgaatcc accagcgcgc gctggtggaa gagatcctcg cagctatagg cggacagtcc
      121 gagctcgggg aacaccgcga gcaccgcctt ctcgcgcgcc gcttcgcgca tgagctcgag
      181 ggtggcgtcg cggttgtagt ccgggtcggc gacgcgcacc agcggcgtgg cgaccgccat
      241 gcgggcgaag ccgtggcgat acaggttgaa gaagtctctc acgaggcaat tctacaggtg
      301 ccgctatatg gtacattaag ccatatgttg ctcctgcgcg aaaccgtgct gacgcgcgcc
      361 ggcacgctcg aggtcgagct gcacgacgac ttgcgctacc ggctgagctg gaaaggcgtg
      421 gtgtacgacc acaagcggcg ctacgacttc aggtcggtcg agcagctgcg ctacgacttc
      481 gagcgcgatg tcgagaaagc ttgagattcg catcggcgcc gccgccgatg cgctcgaccg
      541 cttcgaagcg gcgtggaacc gcgtgcgcga gggccgcgcc cagcggccgc tcgaagtgct
      601 gagcttcgag gatctgccgc tgcttacgaa gacgctttct ccggcgcgct ggacgctgct
      661 gcgggacctg agggaaaaag gcgcctgctc gatctacgag ctggcgaagc gcctggggcg
      721 cgactacaag aacgtgcaca ccgacgtgac gcagttggcg gccatcggcc tggtcgagcg
      781 ccgcgccgac ggcctcgccg cggtaacgtg ggatttgttg cgcgccgagc tcgaacttta
      841 gtccgactgt agcaaccttc cctaggcgca atccgtcaaa cgggcgcagt atcctgctcg
      901 catgaaccgc gatctctcgg ccaccagcgc gctcaacctc gagccgtact ggatgccgtt
      961 caccgacaac cggcgcttca aggcgaagcc caagctcgtc gtcgcggcga aggacatgca
     1021 cttcaccacc gccgaggggc ggcaggtgct cgacgggctg gcgacgctgt ggtgcgtgaa
     1081 cgccggacac gggcgagcgc gcatcgtgga ggcgatccgc cggcaggcgg gcgagctcga
     1141 cttcgcggcg tcgttctcgc tcgggcatcc aggtgcgttc cggctcgcag agcgcgtcgc
     1201 ggcgctcgcg ccgagcggcc tgggccacgt cttcttcacc aactcgggtt ccgaggcgat
     1261 cgacaccgcg atgaagatcg ccgtgggcta ccaccggctg cgcggcgaag gcacccggac
     1321 ccgcttcgtc ggccgcgagc gttcgtacca cggcgtcaac attggcggga tctcgcttgg
     1381 cggcgtgccg gcgaaccgca aagcctacgc cggggtgctc ctcgccggtg tggatcatct
     1441 gcgccacacg cacctcccgg aaaacgcttt cacgaaaggg cagccggcgc gcggcgccga
     1501 gctcgcggat gagctcgagc gcatcgtcgc gctgcacgac gcgtcgaaca tcgccgcgct
     1561 cgtggtggac ccggtatccg cctcgggcgg cgtgctgatt ccgcccaagg gctacctcga
     1621 gcgcctgcgc gagctctgca cccggcacgg gatccttctg gtgttcgacg aggtgatcac
     1681 cggctgggga cggctcggca cgccgttcgc cgcgcagcat ttcggtgtcg agcctgacct
     1741 catcaccttt gcgaagggca tcacttcggg cgcagtgccg ctcggcggcg tgatcgttaa
     1801 ggattcgatc taccagacct tcaccggggc gcaggggccc gggatcgagt ttttccacgg
     1861 ctacacctac tcggggcatc cgctcgcctg cgccgcgggc atcgcggccc tcgataccta
     1921 cgaggaagaa ggcctcttca agcgtggcgc cgccctcgcg aaggatttcg aagacgcaat
     1981 tcactcgctg aaggggctgc cgaacgtcgt cgactgccgc aacctcggcc tgatcggcgc
     2041 ggtggagctc gcgcagcgcc cgggcgcgcc ctacggcacg cgcgcgttcg aggtgttcga
     2101 gaagtgctgg gacaagggcg tgttcgtgcg cccggtgggc gaggcgctcg ccttctgccc
     2161 gccgctcatc gcgcagaaga agcacctcga cctcatgttc ggggtcgtgg gcgaggtgct
     2221 gaaggaaatc gcctgatcta gcggttggcc cgctggagca ccacgttggc aagcacgttg
     2281 accgcgggcg cggtgcgttc gaggtcggcg tgctccttgt tgttgtgtgt gatgccgtcc
     2341 ttgcagggcg agaacatcat cgccgtgggc gcgacgcgcg ccacgtgata ggcgtcatgg
     2401 ccggcctgcg aaggcagatc gagggaggcg acgccgagtc gccgcgcggt gtcgcgcacg
     2461 agcgcaatgc attccgggtc gaaaagctcg gcgttccagg tccactcctc gacgatgcgc
     2521 atgtcgacgt tcgcgcgccg cgcgcagtcg gccatggcgg tc
//
LOCUS       DASUNT010000427         2259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_761543, whole genome shotgun sequence.
ACCESSION   DASUNT010000427 DASUNT010000000
VERSION     DASUNT010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761543"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1628
                     /gene="argS"
                     /locus_tag="VFX94_08680"
     CDS             72..1628
                     /gene="argS"
                     /locus_tag="VFX94_08680"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX5768295.1"
                     /translation="MVFEKPRQPEHGDFACSIALQVAKRLKRNPRQLAEALTAQALAA
                     FPDFEKFFAGYEIAGPGFINFRLRAEVKLASIRAALAAGTSWGRATRDDPLSVQVEFV
                     SANPTGPLHVGHGRQAAIGDALAAILEARGDKVTREFYYNDAGAQIHNLALSVQARAR
                     GSKPGDAGWPADGYAGEYIEDIARQYRGSLDDVEAIRKFAVDYLRREQDIDLREFGVK
                     FDVYYLESSLYTEGKVDEVVKALMASGKTYEKDGALWLRSTEYGDDKDRVIRKSDGTY
                     TYFVPDVAYHLTKWRRGFAKVINVQGTDHMGTKARVRAGLQALNVGVPPGYPDYVMHS
                     MVRVMRGGEEVKISKRAGSYVTLRDLLDWAGRDAVRFFLLSRRADADFVFDIDLARAQ
                     TDENPVYYVQYAHARVCSVFAQWNGNPAGLAQAELSSLANEKEVSLAWRIGEFPDLVA
                     EAARELAPHAITFYLRSLAAEFHSYYNAERILVDDEALRTARLALCAAVRQTLESGLS
                     LIGVGAPEKM"
     gene            1647..2240
                     /locus_tag="VFX94_08685"
     CDS             1647..2240
                     /locus_tag="VFX94_08685"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX5768296.1"
                     /translation="MTHKPRKREAGNFLAGMGVGVLIGLAVSLGIAFYLNRTPIPFTS
                     AKPKQPERGAATEKPPAIAGLPQNAPPAPISAVEKPKFDFYRILPGSEEQVTEKDLRE
                     RERTRAKAGQQDSAKDVYFIQAGSFQNPAEADNQKARLAILGFESSVEPANLPDKGTW
                     YRVRMGPYAKLDDINKVRQALAQNGIQASLIKVKDAQ"
BASE COUNT          425 a          736 c          755 g          343 t
ORIGIN      
        1 cgaaagggcg gctcgccgaa gcgtatcggc aagcgcttgc gaaagaggcg ccgggcgagc
       61 ccgggtcgca gctggtgttc gaaaagccgc gccagccgga gcacggcgac ttcgcctgct
      121 cgattgcgct ccaggtcgcc aagcggctca agcgcaaccc gcgccagctc gcggaggccc
      181 ttaccgccca agcgctggcg gcgtttcccg acttcgagaa gttctttgcc ggctacgaga
      241 tcgcaggtcc gggcttcatt aatttccggc tgagagcaga ggtgaagctc gcgtcgatcc
      301 gggccgcact agccgcagga acgagctggg gacgggcaac cagggacgac ccgctttccg
      361 ttcaggtgga attcgtctca gccaatccca ccgggccctt gcacgtcggc cacgggcggc
      421 aggcggcgat cggcgacgcg ctcgccgcga tcctggaagc gcgcggcgac aaggtgacgc
      481 gcgagttcta ctacaacgac gccggcgcgc agatccacaa cctcgctctc tcggtgcagg
      541 cgcgcgcgcg cggctcgaag cccggcgatg ccggctggcc ggcggacgga tacgccggcg
      601 agtacataga ggacatcgcg cgccagtatc gcggcagcct ggacgacgtc gaggcgattc
      661 gaaagttcgc cgtggactac ctgcgcagag agcaggacat cgatctgcgg gaattcggcg
      721 tcaagttcga cgtctactac ctcgagtcca gcctctacac cgagggcaag gtcgacgaag
      781 tggtgaaggc gctgatggcg agcggcaaga cgtacgagaa ggacggcgcg ctgtggctgc
      841 gcagcaccga gtatggcgac gacaaggacc gtgtgatccg gaagtcggac gggacttata
      901 cctatttcgt ccccgacgtc gcctatcacc tcaccaagtg gcggcgcggc ttcgcgaagg
      961 tcatcaacgt gcagggcacc gaccatatgg gcaccaaggc gcgcgtgcgc gccggcctgc
     1021 aggcgctcaa cgtgggcgtg ccgccgggct acccggatta cgtgatgcac agcatggtgc
     1081 gcgtgatgcg cggcggggaa gaggtgaaga tctccaagcg cgccggctcc tacgtgacgc
     1141 tgcgcgacct gctcgattgg gcaggacgcg acgcggtgcg tttctttctc ctctcacggc
     1201 gcgccgatgc ggatttcgtg ttcgacatcg atctcgctcg cgcccagacc gacgagaacc
     1261 cggtctacta cgtgcaatac gcgcatgcgc gggtatgcag cgtttttgct caatggaacg
     1321 gaaatcccgc cgggctcgcg caggcggagc tctcgtccct cgcgaacgag aaggaagtct
     1381 cgctcgcgtg gcgtatcggc gagttcccgg atctcgtggc cgaagcggcg cgcgagctcg
     1441 cgccgcacgc catcaccttc tacctgcgca gcctcgcggc cgagtttcac agctactata
     1501 atgccgagcg catcctcgtc gacgacgagg cgctgcgcac ggcgcggctc gcgctctgcg
     1561 cggcagtccg ccagacgctg gaaagcgggt tgtcgctcat tggcgtcggc gccccggaga
     1621 agatgtaaat gcggatgtcg cgatcgatga cccacaagcc gcgcaagcgc gaggcaggca
     1681 acttcctcgc cggcatgggc gtcggggtgc tgatcggcct tgcggtgtcg ctcggtatcg
     1741 cgttctatct gaaccgcacg ccgattccgt tcacgagcgc gaagccgaag cagcccgaaa
     1801 ggggtgcggc caccgagaag ccgccggcga ttgccggcct gccgcagaac gcgccccctg
     1861 cgccgatctc cgcggtcgaa aagcccaagt tcgacttcta ccgcatcctg cccggctccg
     1921 aggagcaagt cacggaaaag gacctgcgcg agcgcgagcg aacgcgcgcg aaagccggtc
     1981 aacaggattc tgccaaggac gtttatttca tccaggcagg ctcgtttcag aaccccgccg
     2041 aggcggacaa ccagaaggcc cggctcgcca tcctcggctt cgagtcgagc gtcgagccgg
     2101 ccaacctgcc ggacaaggga acctggtacc gcgtgcggat ggggccgtac gccaagctcg
     2161 acgacatcaa caaggtgcgt caggcgctgg cgcagaacgg catccaagcg agcctgatca
     2221 aggtaaagga cgcgcaatga accgaacacg ccgcgcgct
//
LOCUS       DASUNT010000428         3119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_586004, whole genome shotgun sequence.
ACCESSION   DASUNT010000428 DASUNT010000000
VERSION     DASUNT010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586004"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VFX94_08690"
     CDS             complement(<1..575)
                     /locus_tag="VFX94_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX5768297.1"
                     /translation="MTLTELRYIVAVARERHFGRAAETCFVSQPTLSVAVKKLEEELG
                     VTLFERGPGEVSVTPAGEKIVEQAQRVLEEASRIREIAAAGKDPLAGPLRLGAIYTIG
                     PYLLPKLIPILRRTAPAMQLLIQENFTHRLAEELKSGEVDVILIALPFEAPGVVTRAV
                     YDEPFLVAVPKGHPWENRKRVTSDELASESLL"
     gene            complement(572..1912)
                     /locus_tag="VFX94_08695"
     CDS             complement(572..1912)
                     /locus_tag="VFX94_08695"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX5768298.1"
                     /translation="MDRNKSEVKPKELLSVVDGIFLTAGMIIGALIFKAPSTVAGATS
                     GSGMFLAAWLLGAVVSICGALVYAELASRHPDTGGEYAFLSRGWGKGVAFVFAWSRMS
                     VIQTGAIAAVAFVFGDYASEMLRLGGKSSAVWAALAVVVLTLLNIAGTMQSKTLQKVM
                     EVALIAGLVLFSVAALVTGGQPKPATGGGGSLASFGFAMIFVLLAYGGWNEAAYLAGE
                     VRNARRNMTRVLVGGVLIVAALYILVNLAYLAVLGHGGIKESKAVAADVMRAIAGDRA
                     AVLLALLVCVSVLTTMNAAIFTGARTTYAMGRDFGMFRRLGAWRERGSTPANALMLQG
                     VITLVLVAASAATPDGFSAMVAYTSPVFWTFFLLTGLTLFIFRRKGGEHPVFRVPLHP
                     VVPGLFVLMCAYMLYSSIDYVRNPDYGPKFGFAVLAGLVVMVAGIPLYFLSRKK"
     gene            complement(1888..2742)
                     /locus_tag="VFX94_08700"
     CDS             complement(1888..2742)
                     /locus_tag="VFX94_08700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5768299.1"
                     /translation="MKATVLIAACLAAPAMAQPQTWSWDDGTVPYVQTPMEIVERMLR
                     MAEVKRGDYLIDLGSGDGRIVIEAAKRGARALGVDIDPNLVRHATENAEKAGVADRTQ
                     FVVKDIFETDLSGASVVAFYLLPEFNAKLLPKLLALRPGTRIVSHDGGIGDWPPDERL
                     EMRAPEKPVGVGGVSRVELWIVPADARGTWTADMPGHGGSWTFRVKQQFQILDIDVAA
                     QGRDLLVRNSRLRGEELKIIVTGVVNKRAWHHYFVGELRGDRIAGEVTITDGNNKRSF
                     PWTATRAK"
     gene            2864..2939
                     /locus_tag="VFX94_08705"
     tRNA            2864..2939
                     /locus_tag="VFX94_08705"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2897..2899,aa:Arg,seq:cct)
BASE COUNT          548 a         1065 c         1006 g          500 t
ORIGIN      
        1 agcaggcttt cgctcgcgag ctcatcggag gtcacgcgct tgcggttctc ccacgggtgc
       61 cccttgggaa cggcgaccag gaacggctcg tcgtacaccg cgcgagtgac cacccccggc
      121 gcctcgaagg gtagcgcgat caggatcacg tcgacctcgc cgctcttcaa ctcttcggca
      181 aggcgatggg tgaaattttc ctggatgagg agctgcatcg cgggcgcggt gcgtcgcaga
      241 atcgggatca gcttgggcag caggtaaggg ccgatggtgt agatggcgcc caggcgcagc
      301 ggccccgcga gcgggtcctt gcccgccgcc gcgatttcgc ggatgcgcga tgcctcttcc
      361 agcacgcgct gcgcctgctc gacgatcttc tcgcccgccg gggtcacgct cacctcgccc
      421 ggcccgcgct cgaacagcgt caccccgagc tcctcctcga gcttctttac cgccaccgac
      481 agcgtcggct gcgacacgaa gcatgtttcc gctgcccggc cgaagtggcg ctcgcgcgcc
      541 accgcgacga tgtagcgcag ctcggtgagg gtcatttctt ccgggaaagg aagtacagcg
      601 gtattccggc caccatgacc acgaggccag ccaggaccgc gaagccgaac ttcgggccgt
      661 agtcggggtt gcgcacgtag tcgatgctcg aatagagcat gtaggcgcac atcagcacga
      721 ataggccggg cacgacggga tggagcggca cccggaacac ggggtgctcg ccgcccttgc
      781 gccgaaagat aaacagggtg aggccggtca gcagaaaaaa tgtccagaac acgggcgagg
      841 tataggccac catggcgctg aagccgtctg gcgtggcagc cgaagctgcg acgagcacga
      901 gcgtgattac tccctgcagc ataagcgcat tggccggggt gctgccgcgc tcgcgccacg
      961 ccccgagcct tcggaacatg ccaaagtcgc ggcccatcgc gtacgtggtg cgcgcgccgg
     1021 tgaagatcgc ggcgttcatg gtggtaagga cggagacgca cacgagcagg gcgagcagca
     1081 ccgcggcgcg atcgcccgcg atggcgcgca tcacgtccgc agccaccgct ttcgactcct
     1141 tgatgccccc gtgcccgagc acggcgagat aggcgaggtt caccaatatg taaagcgcgg
     1201 caacaatcaa cactccgccc accagaacgc gcgtcatgtt gcgccgcgcg ttgcgcactt
     1261 cgcccgccag gtaggcggcc tcattccagc cgccataggc gagcagcacg aagatcatgg
     1321 cgaagccgaa ggaagcgagc gaaccgccgc cgccggtcgc gggcttgggc tggccgccgg
     1381 tcacgagcgc ggcgaccgag aagagcacca gcccggcgat aagcgcgacc tccatcactt
     1441 tttgcagcgt cttcgactgc atggtgccgg cgatgttgag cagcgtgagc accacgaccg
     1501 cgagcgcagc ccatacggcg gagctttttc ctcccagccg cagcatctcg gatgcgtagt
     1561 cgccgaacac gaaagcgacc gcggcgatgg cgcccgtctg gatcacgctc atgcgcgacc
     1621 aggcgaacac gaacgcgacc cccttgcccc agccgcgtga aagaaacgcg tactcgcccc
     1681 cggtatcggg atggcgcgag gcgagctccg catacaccag cgcgccgcag atcgatacca
     1741 ccgcgccgag cagccacgca gcgaggaaca tcccgctgcc cgaggtggcc ccggcaaccg
     1801 tggagggcgc cttgaaaatc agcgcgccga tgatcatccc ggccgtcagg aagatgccgt
     1861 cgacgaccga aagcagctcc ttcggcttca cttcgctctt gttgcggtcc aagggaaact
     1921 cctcttgttg ttgccgtcgg taatcgtcac ctcgcccgcg atgcggtcgc ctctcagctc
     1981 gccgacgaag taatggtgcc aggcgcgttt gttcaccacg ccggtcacga tgatcttgag
     2041 ctcctcgccg cgcagccggc tgttccgcac aaggagatcc ctgccctgcg cggcgacgtc
     2101 gatgtcgagg atctggaact gctgcttgac gcggaacgtc caagagccgc cgtggcccgg
     2161 catgtcggcc gtccaggtgc cgcgcgcgtc cgccggcacg atccagagct ccacgcggga
     2221 cacgccgccc accccgaccg gcttttcggg cgcgcgcatc tcgaggcgct cgtcgggcgg
     2281 ccagtcgccg atgccgccgt cgtgcgacac gatgcgcgtg ccgggcctga gcgcgagcag
     2341 cttcggcagg agcttcgcgt tgaactcggg tagaaggtag aacgccacca cgctcgcccc
     2401 ggagagatcg gtctcgaaga tgtccttcac cacgaactgc gtgcggtccg ccaccccggc
     2461 cttttctgcg ttctcggtcg cgtggcgcac gaggttggga tcgatgtcca ccccgagcgc
     2521 gcgcgcgccg cgcttggccg cttcgatcac gatgcgcccg tcgcccgagc cgaggtcaat
     2581 gaggtagtcg cccctcttca cctcggccat gcgcagcatg cgctccacga tctccatcgg
     2641 cgtctgcacg tacggcaccg tgccgtcgtc ccacgaccag gtctgcggct gcgccatcgc
     2701 cggggccgcc aggcaggcgg ctatcaacac tgtcgctttc ataaagggag cctaagtcta
     2761 ccggcacacc gccccacgtt gcagagcgtt tcgcgtagtt tcgagagctg ggcgagcgcg
     2821 ctcaccgacg ggattggcac gcggccgcta gaatgacggc cacgccccgg tagctcagtg
     2881 gatagagcag ccccctccta aggggcaggt cggacgttcg attcgtctcc ggggtgccaa
     2941 ctgattcaaa cagttacgcg cctgtaggca agcgtgaaag ccgtgttcag cacgcatcta
     3001 gcacactgga ggtgtcatgg caccgcccca tctcggcctg ccagcgcgag gcgatggcgc
     3061 gcccttcttc gcggacgctg tgttcatgag ggctttgatt gcttcgtggg tggcttggt
//
LOCUS       DASUNT010000429         8007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_761765, whole genome shotgun sequence.
ACCESSION   DASUNT010000429 DASUNT010000000
VERSION     DASUNT010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8007
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761765"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VFX94_08710"
     CDS             complement(<1..512)
                     /locus_tag="VFX94_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5768300.1"
                     /translation="MKLKFLAAALSIAAPMAVQGQSWEPTKNVEFIIPAGTGGGADQM
                     ARAIQGIVAKHGLMKQSILPINKAGGAGAEGFLDVKNASGDPHKIIITLSNLFTTPLA
                     TGVPFNWRDLTPVQMLALDNFVLWVNAKTPYNSAKDYIEAAKKAGPGKMKMGGTGAKQ
                     EDQIITVAIE"
     gene            complement(615..1376)
                     /locus_tag="VFX94_08715"
     CDS             complement(615..1376)
                     /locus_tag="VFX94_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX5768301.1"
                     /translation="MLEELGTQAFWIGLAKIIGVNIVLSGDNAVVIALAARSLPAKQQ
                     KAAVLWGSGAAVVMRIILTVFAVALLTLPWLKLIGSLLLFWIGVKLLIPEESTEDVDA
                     SENLIAAIKTILIADLVMSIDNVIAVAAAAGGSLTLLILGLAISIPLVIFGSTLLLHL
                     MERWPIIITIGGGLLGFVAGEMLVTDPALKDWLAGLGVAFEGEKPKLAGISLELICGV
                     IGAVIVIVAGTLLGKRRAMKAQVHEAAAESEPPKT"
     gene            complement(1386..3056)
                     /locus_tag="VFX94_08720"
     CDS             complement(1386..3056)
                     /locus_tag="VFX94_08720"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX5768302.1"
                     /translation="MASDIEIAQAAKMQRIAAVAREKLGIAEEHLEPYGHYKAKVSLK
                     YLDTLKGKKDGKLILVTAMTPTPAGEGKTTTTVGLGDALNHIGKKAVICLREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFGAIQLANNLLAAMLDNHIHHGNELGLDVR
                     RIQWRRVLDMNDRALRDIVVSLGGPGNGYPREDGFDIVVASEVMAIFCLATSLEDLKK
                     RLGNIVVGYTREQKPVTAKDLNAHGAMTVLLRDALAPNLVQTLENNPAFIHGGPFANI
                     AHGSNSVIATQAALKLADYVVTEAGFGADLGAEKFLDIKCRKSGLRPNAAVLVATIRA
                     LKYHGGVDVKEVSKENLAALEKGLVNLERHVENVSRVYGIPCVVSFNRFTHDTTAEID
                     LVKSKVKNLGVPFVMATHWGEGGKGAADLARAVVELCDKSAKPAFVYEDSDPLWEKIR
                     KIATKVYRAADVSADAKVRAQIRRLQDDGYGHYPVCVAKTQYSFAADASLRAAPSGHV
                     VNVREVRLAAGAEFVVMICGDIMTMPGLPKVPSANKIDLVDGKVVGLF"
     gene            complement(3081..3509)
                     /locus_tag="VFX94_08725"
     CDS             complement(3081..3509)
                     /locus_tag="VFX94_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5768303.1"
                     /translation="MKILVAVDGSKPSLKAVQLLVEHSDWYRSPPEVELVTVHLPVPQ
                     LPRMGAAVGKAQIEKYYREEGEAMLASARRQLDSARIPYQSHVLVGPVAEAIVKHAKD
                     KRCDLIYVGTQGRSAIGKALIGSTATKVLHISDVPVLLVK"
     gene            complement(3542..6400)
                     /gene="fdhF"
                     /locus_tag="VFX94_08730"
     CDS             complement(3542..6400)
                     /gene="fdhF"
                     /locus_tag="VFX94_08730"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX5768304.1"
                     /translation="MERVKDSQLAMMPIEFKLNGETVVGRPDEPIITTAKKHGVDIPH
                     LCHGGNQRSDGNCRACVVEIKGERVLAPSCCRFPTQGMEVTTNSPRALASQKMVLELL
                     LSDMPQQEYTLDNKLKQWAGRLGVGRSRFRKRSQPKADLSHPAMAVNLDACIQCTRCV
                     RACREVQVNDVIGYAGRGDRSKIVFDFDDPMGESTCVACGECVQACPTGALMPAREVG
                     LKKIDKTVDSVCPFCGVGLQKADKTIDSVCPFCGVGCLLTYHVRQNTILFVNGKDGPA
                     NRNRLCVKGRYGFDYVHHPHRLTKPLIRKPGVKKSAHFVVDPGNWSEVFREASWEEAL
                     QQAADGLKRVRDEDRYALAGFGSAKGSNEEAYLFQKLVRTGFGTNNVDHCTRLCHASS
                     VAALLEGIGSGAVSNQVNDVANAELIFLIGANPTSNHPVAATWMKNAAKRGVKLVYAD
                     PRRSELSRHAWRMLQFKPDTDVALLNAMIHTIIDENLVNEAFVRDRTSGFDALKQNAR
                     NFSPEKMAPVCGIPAETIREVARAFASSKASMILWGMGISQHIHGTDNARCLIALTLM
                     TGQVGRPGTGLHPLRGQNNVQGASDSGLIPMMYPDYQRVSEPEAQNRFEKFWGSALDP
                     KPGLTVVEIMNAAYDGKIRGMYIMGENPAMSDPDLEHARGAMAKLEHLVVQDLFLTET
                     AYLADVVLPATAWPEKDGTVTNTDRMVQLGRKALQPPGEAREDLWIIVEIARRLGLPW
                     SYSKAKDVWNEMRQCMDSISGITWERLERDSSVTYPCENEGDPGQPVVFQTHFPTATG
                     RGKFVPADLIPAAERPDGEYPMVLITGRQLEHWHTGAMTRRSGALDAIEPEPVASLHP
                     LDLQELKAAPGDLVTVESRRGKISLYARADAGTPRGAVFIPFCYYEAAANLLTNPVLD
                     PFGKIPEFKYCAVKVLKGGARPGDPGYGKGRTLAELYR"
     gene            complement(6400..>8007)
                     /locus_tag="VFX94_08735"
     CDS             complement(6400..>8007)
                     /locus_tag="VFX94_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX5768305.1"
                     /translation="DPTALQEVQALLRDAPRRPDLLIEHLHRINDRYGCLGAAHLAAL
                     AQEMGLALAEVYEVATFYHHFEVVKEGSRAPAPLTVRVCDSIACELAGAQQLLKNLSF
                     GSEVRVIPAPCVGRCEVAPCAVVHQNPVGNATVESVKRAVAEKQLSCPQTGYVEYAQY
                     RKGGGYRLIAECLAGKRSREDITRIMEDSGLRGLGGAGFPAGRKWRLVAAEPEPRVMA
                     VNIDEGEPGTFKDRWYLERDPHRFLEGMLVAAWCAGVGEIWIYLRDEYAGCREILEKE
                     LPRLKKEAPCALPPIHLRRGAGAYICGEESAMIESIEGKRGMPRLRPPYVAQVGLFGR
                     PTLEHNMETVYWVREIVEKGAQWFAAQGRNGRKGLRSFSVSGRVKKPGVHIAPAGITL
                     KELIDEYCGGMLGGHELYAYLPGGASGGILPASKADIPLDFDTLQPHGCFIGSAAIVV
                     LSDKDKAARAAVNLMKFFHDESCGKCTPCRVGTAKAVTLMEERKWNQPLLQELSQAMM
                     DASICGLGQAAPNPALSVMKYFPHEVQ"
BASE COUNT         1245 a         2678 c         2582 g         1502 t
ORIGIN      
        1 tgctcgatcg ccacggtgat gatctggtct tcctgcttcg cccccgtgcc gcccatcttc
       61 atcttgcccg gccccgcctt cttcgcggcc tcgatgtagt ccttcgcgct gttgtacggc
      121 gtcttcgcgt tcacccacag aacaaagttg tcgagcgcca gcatctgcac cggagtgagg
      181 tcgcgccagt tgaacggcac accggtcgcg agcggcgtgg tgaagaggtt ggacagcgtg
      241 atgatgatct tgtgcgggtc gcccgaagcg ttcttcacgt cgagaaagcc ctcggcgccc
      301 gcgccgcccg ccttgttgat cggcaggatc gattgcttca tcaggccgtg cttggcgacg
      361 atgccctgga tcgcgcgcgc catctggtcg gcgccgcctc cggttcccgc ggggatgatg
      421 aactcgacgt tcttggtcgg ctcccaggat tgcccctgga ccgccatcgg cgctgcgatg
      481 gaaagtgctg cggcaaggaa cttcagtttc atggctcctc ctaaagttgc acccttcgtt
      541 gcgccgcacc attcgaacgt ggcgctggcg cctggattct catgctccct cttaagcccg
      601 ggccatgatc tccgtcaagt cttcggcggt tcgctttcgg ccgcggcctc gtgcacttgc
      661 gccttcatgg ccctgcgctt gccgagcaac gttccggcga cgattacgat gacagcgccg
      721 atcacgccgc agatgagctc gaggctgatg ccggcgagct tgggcttttc gccttcgaag
      781 gcgacgccca ggcccgccag ccagtccttg agcgccgggt cggtgacgag catctcgccc
      841 gcgacgaagc cgagaaggcc cccgccgatc gtgatgatga tcggccagcg ctccatgagg
      901 tgcagcagca gcgtcgagcc gaagatcacg agcgggatcg agatcgcgag cccgagaatg
      961 agcagcgtca ggctgccgcc ggcggccgcc gcgaccgcga tcacgttgtc gatgctcatc
     1021 acgagatcgg cgatgaggat cgtcttgatc gcggcgatca ggttctcgct cgcgtcgacg
     1081 tcctcggtgc tctcctccgg gatgaggagc tttacgccga tccagaaaag gagcagcgag
     1141 ccgatgagct tgagccacgg cagcgtgagc agcgccaccg cgaacaccgt gaggatgatc
     1201 cgcatcacca ccgcggcgcc cgagccccac agcaccgcgg ccttctgctg cttcgccggc
     1261 agcgagcgcg cggcgagcgc gatcaccacc gcgttgtccc cggagagaac gatattgacg
     1321 ccgatgattt tcgcaaggcc gatccagaac gcctgcgtgc cgagctcctc cagcatcggc
     1381 cgattctaga acagcccgac gactttcccg tccaccaggt cgatcttgtt ggccgagggc
     1441 accttgggaa gccccggcat ggtcatgatg tcaccgcaga tcatcacgac gaactccgcg
     1501 ccggcggcaa ggcgcacctc gcgcacgttg accacgtggc cgctcggcgc cgcgcgcagg
     1561 ctggcatcgg cggcaaagga atactgggtc ttcgccacac acacgggata gtggccgtag
     1621 ccgtcgtcct gcagcctgcg gatctgcgcc cgcaccttcg catccgcgct cacgtcggcg
     1681 gcgcggtaga ccttggtcgc gatcttcctg atcttctccc acagcgggtc ggagtcctcg
     1741 tacacgaacg ccggcttcgc ggacttgtcg cacagctcca ccacggcgcg cgccaggtcg
     1801 gccgcaccct tgccgccttc tccccaatgc gtggccatga cgaagggcac gccgaggttc
     1861 ttgaccttgg acttcacgag gtcgatctcg gccgtcgtgt cgtgggtgaa gcggttgaac
     1921 gacacgacgc acggaatgcc gtaaaccctg gagacgttct cgacgtggcg ctccaggttc
     1981 accagcccct tctcgagcgc cgcgaggttc tccttgctca cttccttcac gtccaccccg
     2041 ccgtggtact tgagcgcgcg gatcgtggcg acgagcaccg cggcgttcgg gcgcaggccg
     2101 ctcttgcggc acttgatatc gaggaatttc tcggcgccaa ggtcggcgcc gaagcccgcc
     2161 tcggtcacca cgtagtcggc gagcttgagc gcggcctgcg tggcgatcac cgagttgctc
     2221 ccgtgcgcga tgttggcgaa cggcccgccg tggatgaagg cggggttgtt ctcgagcgtc
     2281 tgcacgaggt tgggcgcgag cgcatccctg agcagcacgg tcatcgcgcc atgagcattc
     2341 agatctttcg ccgtcaccgg cttctgctca cgggtgtagc ccaccacgat gttccccagc
     2401 cgtttcttca gatcctcgag cgaggtggcg aggcagaaga tcgccatcac ttcggacgcg
     2461 acgacgatat cgaagccgtc ctcccgcggg tagccgttgc ccggcccgcc gagcgacacg
     2521 acgatgtcgc gcagggcgcg gtcgttcatg tccagcacgc gccgccactg gatgcggcgc
     2581 acgtcgaggc cgagctcgtt gccgtggtgg atgtgattgt cgagcatcgc ggcgagcagg
     2641 ttgttcgcga gctggatcgc gccgaagtcg ccggtgaagt gcaggttgat gtcctccatc
     2701 ggcaccacct gcgcgtagcc gccgccggcc gcgccgccct tcacgccgaa cacagggccg
     2761 agcgagggct cgcgcaggca gatcacggcc ttcttgccga tgtggttgag cgcgtcgccc
     2821 agtcccaccg tggtcgtcgt cttgccctcg ccggcgggcg tgggcgtcat cgcggtaacg
     2881 aggatcagct tgccgtcctt cttccccttc agcgtatcga ggtacttcag cgacactttc
     2941 gccttgtaat ggccgtaggg ctcgaggtgc tcttccgcga tgccgagctt ctcgcgcgcc
     3001 accgcggcga tgcgctgcat cttggctgcc tgtgctatct caatatccga ggccatgcgt
     3061 gagtctccag aaagcgcaaa ctatttgacg agcaggaccg ggacgtccga aatgtgcagc
     3121 accttcgtcg cggtggagcc gatgagcgcc ttgccgatcg cgcttctgcc ctgcgtgccg
     3181 acataaatga ggtcgcagcg cttgtccttc gcatgcttga cgatcgcctc ggcgaccggt
     3241 ccgaccagga catgcgactg gtatggaatg cgcgcggaat cgagctgccg gcgtgcgctc
     3301 gcgagcatcg cctcgccttc ctcgcggtag tacttttcga tctgcgcctt gccgaccgcg
     3361 gcgcccatgc gcgggagctg cggcaccggc aggtgcacgg taacgagctc cacctccggc
     3421 gggctgcgat accagtcgga gtgctcgacg agcagttgca cggctttcag cgacggcttg
     3481 gagccgtcga cggcgacgag aattttcatc ttgttgtggt tggggactgg tgcaagcgcc
     3541 cttaccggta aagctccgcg agtgtacgcc ctttgccgta gcccgggtct cccggccgcg
     3601 cgccgccctt cagcactttc acggcgcagt acttgaactc cgggatcttg ccgaacgggt
     3661 cgagcaccgg gttggtgagc agattcgcgg ccgcttcgta gtagcagaac gggatgaaca
     3721 ccgcgccgcg aggcgtgccg gcatcggcgc gcgcgtaaag cgaaattttc ccgcgccggg
     3781 attccacggt caccagatcg cccggggcgg ccttcagctc ctgcagatcc agcggatgca
     3841 gcgatgcgac cggctcgggc tcgatcgcgt cgagcgcgcc cgagcggcgc gtcatcgcgc
     3901 cggtgtgcca gtgctcgagc tgccggccgg tgatgagcac catgggatat tcgccatccg
     3961 gccgctccgc cgcggggatc aggtcggccg gaacgaactt gccgcgcccg gtcgcggtcg
     4021 ggaaatgcgt ctggaacacc accggctggc cggggtcgcc ttcgttctcg cacgggtagg
     4081 tgaccgacga gtcgcgctcg agccgctccc aggtaatgcc gctgatcgaa tccatgcact
     4141 ggcgcatctc gttccagacg tctttcgctt tcgaatacga ccagggcagg ccgaggcgcc
     4201 ttgcgatctc gacgatgatc cagaggtcct cgcgcgcctc gcccggcggc tgcagcgcct
     4261 tgcggccgag ctgcaccatg cggtcggtgt tggtcaccgt gccgtccttc tccggccatg
     4321 ccgtggcggg cagcaccacg tcggcaaggt aagcggtttc cgtgaggaag aggtcctgca
     4381 ccacgagatg ctcgagcttc gccatcgcgc cgcgcgcgtg ctcgagatcg ggatccgaca
     4441 tcgccgggtt ctcccccatg atgtacatgc cgcggatttt tccgtcgtac gcggcgttca
     4501 tgatctcgac cactgtcagc ccgggcttcg gatcgagcgc gctgccccag aatttctcga
     4561 aacgattctg cgcctcgggc tcggagacgc gctggtagtc ggggtacatc atcggaatca
     4621 ggcccgagtc ggacgcaccc tgcacgttgt tctggccgcg cagcgggtgc agccccgttc
     4681 ccggccggcc cacctgcccg gtcatcaagg tgagcgcgat caggcagcgc gcgttgtcgg
     4741 tgccgtggat gtgctgcgag attcccattc cccaaaggat catcgacgct ttggagctcg
     4801 cgaaggcgcg tgccacctcg cggatcgtct cggcgggaat gccgcagacg ggcgccatct
     4861 tctccggcga gaagttcctc gcgttttgct tcagcgcgtc gaatccggaa gttcggtctc
     4921 ttacgaacgc ctcgttgacc agattctcgt cgatgatcgt gtggatcatc gcgttcagga
     4981 gcgcgacgtc cgtgtcgggc ttgaactgca gcatgcgcca cgcgtggcgc gagagctcgc
     5041 tgcgccgcgg gtcggcgtac accagcttga ccccgcgctt cgccgcgttc ttcatccatg
     5101 ttgcggccac cgggtggttg gaggtcgggt tggcgccgat gagaaagatc agctcggcgt
     5161 tcgcgacgtc gttcacctgg ttcgacaccg cgcccgagcc gatcccctcg aggagcgccg
     5221 ctaccgacga agcgtggcaa agacgcgtgc aatggtccac gttgttcgtg ccgaagccgg
     5281 tgcgcacgag cttctggaag aggtacgctt cctcgttcga tccctttgcc gagccgaagc
     5341 cggcgagcgc atagcgatcc tcatccctga cgcgtttcaa cccgtccgcc gcttgttgca
     5401 gtgcctcttc ccagctcgct tcgcggaaca cttcggacca gttgccggga tcgacgacga
     5461 agtgtgccga tttcttcacg ccgggcttgc ggatcagcgg cttggtcagc cggtgcggat
     5521 ggtgcacgta gtcgaacccg tagcggcctt tcacgcaaag gcggttgcgg ttcgccggtc
     5581 cgtccttgcc gttcacgaac aggatcgtgt tctgcctgac gtggtaggtg agcaggcacc
     5641 cgaccccgca gaacgggcac accgagtcga tcgtcttgtc ggccttttgc aggcccacgc
     5701 cgcagaacgg gcaaaccgag tcgaccgtct tgtcgatctt tttcaggccg acctcgcgcg
     5761 ccggcatcag cgcgccggtc gggcaggcct gcacgcactc gccgcaggcg acgcaggtgg
     5821 attcgcccat cgggtcgtcg aagtcgaaca cgatcttgga tcgatcgccg cggccggcat
     5881 agccgatcac gtcgttcacc tgcacttcgc ggcaggcgcg cacgcaacgc gtgcactgga
     5941 tgcacgcatc gaggttcacc gccatggcgg ggtgcgaaag atcggccttg ggctggcttc
     6001 gcttgcggaa tctcgatctt ccaacgccaa ggcggcccgc ccactgcttc agcttgttgt
     6061 cgagcgtgta ttcctgctgc ggcatgtcgg agagcaggag ctcgagcacc atcttctgcg
     6121 aggcaagggc gcgcggcgaa ttggtggtca cctccatgcc ctgggtcggg aagcggcagc
     6181 atgagggcgc gagcacgcgc tcgcccttga tctcgaccac gcaggcacgg cagttgccgt
     6241 cggagcgctg gttgccgccg tggcacaggt gcgggatgtc gacgccgtgc ttcttcgcgg
     6301 tggtgatgat cggctcgtcc ggacggccga caacggtctc gccgttcagc ttgaactcga
     6361 tcggcatcat ggcgagctgc gagtccttca ctcgttccat cactggacct cgtgcgggaa
     6421 gtacttcatt acgctgaggg cgggattcgg cgcggcctgc cccaggccgc agatcgacgc
     6481 gtccatcatt gcctgtgaga gctcctgcag gagcggttgg ttccacttgc gctcttccat
     6541 aagcgttacc gccttggcgg tgccaacgcg gcaaggcgtg cacttgccgc aggactcgtc
     6601 gtggaagaac ttcatcaggt tgaccgccgc cctggctgcc ttgtccttgt cagacagcac
     6661 gacgatcgcc gccgagccga tgaagcagcc gtgcggctgc agcgtatcga agtcgagcgg
     6721 gatgtcggcc ttcgacgcgg gcaggatgcc gccggaggcg ccgccgggca ggtaagcgta
     6781 gagctcatgg ccgccgagca ttcctccgca gtactcatcg atgagttcct tgagagtgat
     6841 tcccgcgggg gcaatatgga ctccgggctt tttcactcgc ccggacaccg aaaacgagcg
     6901 cagccctttg cggccattgc ggccctgcgc ggcaaaccat tgcgcgccct tctccacgat
     6961 ctcgcgcacc cagtacaccg tctccatgtt gtgctcgagg gtgggccggc cgaaaagccc
     7021 gacctgcgcg acgtagggcg gccgcagccg cggcatgccg cgcttgccct cgatcgactc
     7081 gatcatcgcg gattcctcgc cgcagatgta agcccccgca ccgcggcgca ggtggatcgg
     7141 gggtaacgcg cagggcgctt ccttcttcag gcggggaagc tctttctcga ggatctcgcg
     7201 gcagccggcg tactcgtctc gcaggtagat ccagatctcg ccgacgcccg cgcaccaggc
     7261 ggcgacgagc atgccttcga ggaagcggtg cgggtcgcgc tcgagatacc agcgatcctt
     7321 gaacgtgccg ggctcgccct cgtcgatgtt caccgccatg acgcgtggct cgggctcggc
     7381 cgcgaccagg cgccacttgc gtccagccgg aaatccggcc ccgcccagcc cgcgcagccc
     7441 ggagtcctcc atgattctgg tgatgtcttc gcgtgagcgt tttccggcca ggcactcggc
     7501 gatcagcctg tagccaccgc cctttcggta ctgcgcgtac tccacgtatc cggtttgcgg
     7561 acacgacaat tgcttttccg cgaccgcgcg ctttaccgac tcgaccgtcg cgttgccgac
     7621 cggattttgg tgaacgacgg cgcagggcgc gacctcgcat cggccgacac agggtgcggg
     7681 aatgacgcgt acctcgcttc cgaaagacag attcttcaga agctgctggg ctcccgccag
     7741 ctcgcacgca atggagtcgc acacccgcac ggtgagtggc gcgggcgcgc ggctgccttc
     7801 cttcacgact tcgaagtggt gatagaacgt ggccacctcg tacacctccg cgagcgcgag
     7861 ccccatctcc tgcgcgagcg cggcgaggtg cgccgcgccc aggcagccgt agcggtcgtt
     7921 gatccggtgc aggtgctcga tcagcaggtc gggccggcgc ggcgcgtcgc ggagcagcgc
     7981 ctggacctcc tgcaacgccg tcgggtc
//
LOCUS       DASUNT010000430         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_236773, whole genome shotgun sequence.
ACCESSION   DASUNT010000430 DASUNT010000000
VERSION     DASUNT010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236773"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1463
                     /locus_tag="VFX94_08740"
     CDS             <1..1463
                     /locus_tag="VFX94_08740"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX5768306.1"
                     /translation="VAWLTPPEAAGEGEGERTPRAPLAQGDVALWLALSATGSVLLLA
                     VTNHLTQNVASMPLLWLAPLTLYLASFIVTFDGKGWYRPQWLWSVLVVWIAAMGWLIV
                     DLDFQFDLWLQLAIFLPGVFIGCLFCHGELYRLRPPPERLTAFYLIVAAGGALGGILV
                     AVVAPLVFSGYYELGAGLVALAALAAVCFGRLGRVPRYASLALLLGIAACATYDGMRY
                     QRDVRIATRSFYGVLRVKEYGVKGEESHLRRLVHGAIMHGEQYLHEKTRTMLTTYYHQ
                     DSGIGGAILSLGERPARVGIIGLGVGTIAAYGRAGDLYRFYEIDSRVMQVALHEFTYL
                     TDSKAKIELALGDARLALEREPPRNFDVLAVDAFSSDAIPVHLITREALGVYLRHVRP
                     DGIVAFHVSNRFLNLVPVVARLAKEHGAHVVLVRDDPDEEEDYRRSRTDWVLVSRDPA
                     ALAREAIVERGGAAPEDRPEWRTWTDDYSNLIQILK"
     gene            complement(1490..>2015)
                     /gene="secF"
                     /locus_tag="VFX94_08745"
     CDS             complement(1490..>2015)
                     /gene="secF"
                     /locus_tag="VFX94_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX5768307.1"
                     /translation="AVAIVFYLWARFVNWRFGLAAIIANLHDVVIILGFFAFFQWEFS
                     LAVLAAVLAVLGYSVNESVIVFDRVREYFRKMRKASTAEVIDAAITSTISRTIITHGS
                     TQVMATTMLLFGGPALHYFALALTIGICFGIYSSVLVASPLVMWFGARREQFIVAAKK
                     KTTGARDEQEGAVV"
BASE COUNT          308 a          681 c          685 g          341 t
ORIGIN      
        1 ccgtggcgtg gctcacgccg cccgaggccg cgggcgaggg cgagggggag cgcacgccgc
       61 gggcgccgct cgcccagggc gacgtcgccc tgtggcttgc tttgtccgcg acgggctcgg
      121 tgctgctgct tgcggtcacg aaccacctga cgcagaacgt ggcatcgatg ccgctcctgt
      181 ggcttgcgcc gctcacgctc tatctcgcga gcttcatcgt cactttcgac ggcaagggct
      241 ggtaccggcc gcagtggctg tggtccgttc tcgtcgtctg gattgcggcg atgggctggc
      301 tcatcgtcga cctggacttc cagttcgacc tgtggctcca gctggcgatc ttcctgccgg
      361 gcgttttcat cggctgcctg ttctgccatg gggagcttta ccgcctgcgt ccgccgcccg
      421 agcggctcac cgcgttctac ctgatcgtcg ccgccggcgg cgcgctcggc gggatcctgg
      481 tggccgtggt ggcgccgctc gtcttcagcg gctactacga gctcggcgcc ggcctggtgg
      541 cgctggcggc gctcgccgcc gtgtgcttcg ggcgccttgg ccgggtgcca cgctatgcga
      601 gcctggcgct gctgctcggc atcgctgcgt gcgccaccta cgatggcatg cgctaccagc
      661 gcgacgtgcg catcgccaca cgcagcttct acggcgtgct gcgcgtcaag gaatacgggg
      721 tgaagggcga ggaatcacac ctgcgccgcc tggtgcatgg cgcgatcatg cacggggagc
      781 agtacctgca cgagaagacc cgcaccatgc tcactacgta ctatcaccag gactcgggca
      841 tcggcggcgc gatcctctcg ctcggcgagc ggccggcgcg cgtcggcatc atcggcctgg
      901 gcgtggggac catcgccgcg tacgggcgcg cgggggactt gtaccgcttc tacgagatcg
      961 actcgcgcgt catgcaggtc gcgctgcacg agttcaccta cctcaccgac agcaaggcga
     1021 agatcgagct cgcgctcggc gacgcacgcc ttgcgctcga gcgcgagccg ccgcgcaact
     1081 tcgacgtgct cgcggtcgat gcgttttcga gcgatgccat tccggtacac ctcatcacgc
     1141 gggaggcgct cggcgtgtac ttgcgccatg tcaggcccga cggcatcgtc gccttccatg
     1201 tgtccaaccg cttcctcaac cttgtcccgg tcgtcgcgcg gcttgcgaaa gagcacggcg
     1261 cgcacgtggt tctggtccgc gatgaccccg acgaggaaga ggactaccgg cgctcgcgca
     1321 ccgactgggt gctcgtgtca cgcgaccccg cggcgctcgc ccgcgaggcc atcgtcgagc
     1381 gcggcggggc agcgccggag gaccgccccg agtggcggac ctggaccgac gactattcca
     1441 acctaataca gatcctcaag tagctcgaaa aaggggtcag gacccctttt cagacgaccg
     1501 cgccttcctg ctcgtcgcgg gctccggtgg ttttcttctt ggcggcgacg atgaactgct
     1561 cgcgcctcgc gccgaaccac atgacgagcg ggctcgccac gagcacggaa gagtagatgc
     1621 cgaagcagat gccaatggtg agggcgagcg cgaagtagtg cagcgccggg ccgccgaaga
     1681 gaagcatggt ggtcgccatc acctgggtgc tgccgtgggt gatgatggtg cgcgagatgg
     1741 tgctcgtgat ggcggcgtcg atcacctccg cggtggacgc cttgcgcatc ttgcggaagt
     1801 actcgcgcac ccggtcgaac acgatcaccg actcgttcac cgagtagccg agcacggcga
     1861 gcaccgccgc cagcaccgcg agcgagaact cccactggaa gaaggcgaag aagccgagga
     1921 tgatcaccac gtcgtgcagg ttggcgatga tcgcggccag cccgaagcgc cagttgacga
     1981 agcgcgccca caggtagaag acgatcgcca ccgcc
//
LOCUS       DASUNT010000431         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_586343, whole genome shotgun sequence.
ACCESSION   DASUNT010000431 DASUNT010000000
VERSION     DASUNT010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586343"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VFX94_08750"
     CDS             complement(<1..1015)
                     /locus_tag="VFX94_08750"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX5768308.1"
                     /translation="MHLAVVLGLCALLAAAPAPASDECLACHGQQGLSKTFPGGETLA
                     LHVDAAAFARSVHAPVGCAACHAQVDLKTHPAKTREFPSVRAYAIAAVEACRGCHEAI
                     YDAWLGSQHGKTRSRTSGLQGPICADCHRPHEVTPASVGTRLKDTCLTCHAGAPEAHA
                     AWLPNSKRHLAVVACAACHAPEAKRKVDLRFYDASGKNEVIGGYGELQPGMPLDEKRL
                     WALMHGAGGKVRLVGRIEVASADQAHAMADSGQALKECTTCHRKGAEAFQNVTVSVVG
                     PDGKRVHYEAQKEVLSAPSSVDTVRGFYAIGGTRVGALDVLLLLVLVGGMAAPFFHFV
                     LRRL"
     gene            complement(1058..1609)
                     /locus_tag="VFX94_08755"
     CDS             complement(1058..1609)
                     /locus_tag="VFX94_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5768309.1"
                     /translation="MNALKKILVVDDDPVVGRSFDRVLSPKGYAVISATSGAEALERL
                     EAEKYDMVYTDIKMPGMDGIEVAKRIKAGRPWLPVVIVTGYGTEANEAEARAAGVAEF
                     LHKPLSPETIEGSAHFAYSAVALPAPAAATSPMPARSVGRLLKNVGLFFAAPFIGLAY
                     VVLLPFLGIGVAGWAVYKAIKHR"
     gene            complement(1606..2019)
                     /locus_tag="VFX94_08760"
     CDS             complement(1606..2019)
                     /locus_tag="VFX94_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5768310.1"
                     /translation="MTNNNKARVLVVDDEPVVRMAYTRSLGAEFEPHTVSDGEEALRA
                     MERTPADIVLLDLRLPGAQGLEVLEAMKRKWPETEVVVITGYPSVDSAKQAVRLGAAD
                     YLAKPVGTDEVMSAARNALTRKLWTLRIQPERSIS"
BASE COUNT          296 a          733 c          732 g          344 t
ORIGIN      
        1 tgagcctgcg tagcacgaag tggaagaacg gtgccgccat gccgccgacg agcacgagaa
       61 gcagcagcac gtcgagcgcc ccgacgcgcg tgccgccgat ggcgtagaag ccgcgcaccg
      121 tgtcgaccga gctcggcgcg ctgagcacct ccttttgcgc ttcgtagtgc acgcgcttgc
      181 cgtccgggcc gacgaccgag acggtgacgt tctggaacgc ttcggcgccc ttgcggtggc
      241 aggtggtgca ttccttcagc gcctggccgc tgtcggccat ggcgtgcgcc tggtcggcgc
      301 tcgcgacttc gatgcggccg acgagccgca ccttgccgcc ggcgccgtgc atgagcgccc
      361 agaggcgctt ctcgtcgagc ggcatgccgg gctgcagctc gccgtagccg ccgatcacct
      421 cgttctttcc gctcgcgtcg tagaagcgca ggtccacctt gcgcttggct tccggcgcat
      481 ggcaggccgc gcaggcaacg accgcgagat ggcgcttgct gttcggcagc caggcggcgt
      541 gcgcctcggg cgcgcccgca tggcaggtga ggcaggtgtc tttgaggcgt gtgccgaccg
      601 acgcgggcgt cacttcgtgc gggcggtggc agtcggcgca gatcgggccc tgcagcccgc
      661 tcgtgcggct gcgcgttttt ccgtgctggc tgccgagcca ggcgtcgtag atcgcctcgt
      721 ggcagccgcg gcaggcttcg accgcggcga tcgcataggc gcgcacgctc gggaactcgc
      781 gcgtcttggc gggatgcgtc ttcaggtcga cctgcgcgtg gcaggcggcg cagcccaccg
      841 gggcgtgcac ggagcgggcg aacgccgcag cgtccacgtg cagcgctagc gtctcgccgc
      901 ccgggaaggt cttgctaagg ccctgctggc cgtggcaggc gagacactcg tcgctcgccg
      961 gcgcgggggc agcggcgagc agcgcgcaaa gcccgagtac gaccgcgagg tgcatcccgc
     1021 tagcttaccc aggcgtgccg ccggcatttg acgtggatca acgatgcttg atcgccttgt
     1081 acaccgccca gcccgccacg ccgatgccga ggaacggcag cagcaccacg tacgcaaggc
     1141 cgatgaaggg ggcggcgaaa aagagcccca cattcttcag cagccgcccg accgaccgcg
     1201 cgggcatggg ggaagtcgcc gctgccggtg cgggcagggc gaccgcggag taggcgaaat
     1261 gcgcgctgcc ttcgatcgtt tcgggcgaaa gcggcttgtg caggaactcg gctacgcccg
     1321 ccgcacgcgc ctcggcctcg ttcgcttcgg tgccgtaccc ggtgacgatc accaccggca
     1381 gccacgggcg gcccgccttg atgcgcttcg ccacctcgat gccgtccatg ccgggcatct
     1441 tgatgtcggt gtacaccatg tcgtacttct ccgcctcgag gcgctcgagc gcctcggcgc
     1501 cgctcgtggc gctgatcacc gcgtagcctt tcggcgaaag cacccggtcg aagctcctgc
     1561 cgaccaccgg gtcgtcgtcc actaccagga tctttttcaa cgcgttcatg aaatgctcct
     1621 ctcgggttga atgcgcagcg tccaaagctt gcgggtgagc gcgttgcgcg ccgcgctcat
     1681 cacttcgtcg gttccgacag gcttggcgag gtagtcggcc gcgccgaggc gcaccgcctg
     1741 cttcgccgaa tccacgctcg ggtaaccggt aatcaccacc acctcggtct cgggccattt
     1801 gcgcttcatg gcctcgagca cctcgaggcc ttgggcgccc ggcaggcgca gatcgagcag
     1861 cacgatatcg gccggtgtgc gctccatcgc gcgcagcgcc tcctcgccgt cggacacggt
     1921 gtgcggttcg aactctgcgc cgaggctgcg ggtgtaggcc atccgcacga ccggctcgtc
     1981 atcgacgacg agcacgcggg ccttgttgtt gttcgtcatg gcgcgctccg ctcaggcggt
     2041 tcccttgagg acggcgcgag cggccgtcca cgcgagcgcc ccgatgccga cgaagggcag
     2101 cgcgg
//
LOCUS       DASUNT010000432         4347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1112869, whole genome shotgun sequence.
ACCESSION   DASUNT010000432 DASUNT010000000
VERSION     DASUNT010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112869"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..1033
                     /locus_tag="VFX94_08765"
     CDS             251..1033
                     /locus_tag="VFX94_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX5768311.1"
                     /translation="MKAPSFAYAKPRTLAEAFELVERPGAKILAGGQSLIPALNMRLS
                     APELLVDITGLASLRGVTLEQGVVRIGALTTHAGIERSAEIPKHVPLLAEAVRHIAHP
                     AIRNRGTLGGSIALADPAAEYPACLVALEATIVLQSKAGERMVRAEEFFKGLFEVDLA
                     VGELVAAVEIPAQLKDERSVFLELARRHGDYAIVGLAAVTKLSGRRLVFFGLSNKPVL
                     VKPSSLEEVKAELPTPAADLYNSPSTKLHLAKVLLERAWNRL"
     gene            1018..1485
                     /locus_tag="VFX94_08770"
     CDS             1018..1485
                     /locus_tag="VFX94_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5768312.1"
                     /translation="MEQALTITVTVNGTQLTRSVAARQHLVDFLREELGLTGSHIGCE
                     HGVCGACTVRVNGDIVRGCLMLAVQANGCRVDTIEGLSDSKELAKLQKAFHEKNALQC
                     GFCTPGMLMAAQDLVRMNKKFSREEIRSHISGNYCRCTGYQAIVDAIDEVING"
     gene            1478..3865
                     /locus_tag="VFX94_08775"
     CDS             1478..3865
                     /locus_tag="VFX94_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5768313.1"
                     /translation="MGKAELPLHAPGTQLDKGYIGQSVPRANAQRLLQGRGAFTDDLR
                     FPRLAHVAFFRSPYAHARIAKLEFRNALSAPGVIAVVDGRAVAEYCKPWVAVLAHLKG
                     IKSPPQHAIAIERACWQGEACAAVVAESRSQAEDAVALIEAEWEELPVVVDMEAALQG
                     NVVIHEELKDNVCFKRELDTGGVDEAFASADMVVEDTFHFGRHTGVCPEGRAILADYN
                     AAEHSLTVYHATQAPHMMQDIFSRHLGIPEANVRVIAKDVGGSYGIKVHVYADEMATA
                     ALSVMLRRPVKFVADRLESFLSDIHAREHRVKVRLAVKSTGEILAFDLDDLTAIGPYS
                     VYPRTSGIEGNQVVNLTGGPYKHQKYKARMHVVFTNKNVTCQYRAVGHPIAVALTEGI
                     VDLAAQKLGMDPAEFRKKNLIPDEAYPYTFPSGIKFEKLSHHKALDKLLALMNYHKLR
                     EEQKQLRDRSVFRGIGLAAMIEVTNPSPAFYGVGGARISAQDGATLRFEPAGMVTVMC
                     SVTEQGQGTEGIFAQIAASAVGVSMDKVRVITGDTGVTPYGGGTWASRGAGIGGEAVL
                     QAGRALRSNMLDVASRILNLDKAELDIANNAVVSRLTQERKLDLAELGRIAYFRPDTL
                     PIDFQSELTVTRHYTPRQYGFTFTNGIQASLVELDPDTGLVKLLKHWCVEDSGTLINP
                     MLVDEQIRGGVVQGIGAALYEECLYSPDGQLLNGNMADYLVPMSGEMPDIVCAHIETP
                     TLQSQLGAKGVGEAGTAGAPAAVMNAINDALAPLNARVTRMPFTPERILQALGKV"
     gene            complement(3862..4020)
                     /locus_tag="VFX94_08780"
     CDS             complement(3862..4020)
                     /locus_tag="VFX94_08780"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HEX5768314.1"
                     /translation="MEFRRADVTETRDLDKNTQIDLDAAGKLCGLTLEHASERADISA
                     VDYQQVAA"
     gene            4019..>4347
                     /locus_tag="VFX94_08785"
     CDS             4019..>4347
                     /locus_tag="VFX94_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768315.1"
                     /translation="MYSVSVSWKYLIFAVQIFDRRKHYALSRRREHYALLSGRMTTKP
                     KDARVSSAISHWAPRFVSNGVLLADFEDVTGSLERWEDWCAAWSARAKVHEDLGREAL
                     TQEFRMTA"
BASE COUNT          791 a         1394 c         1420 g          742 t
ORIGIN      
        1 acgatcttgc cgccgagctg cgcggcaagc ggctcggcta tgaggcgcgc catgatgtcc
       61 acgctgccgc cgggcgggaa cggcgcgatc agccggaccg gctggctggg gtagggttgg
      121 gctgcagccg agaaggagaa agcaagcgcg gcaagcggca cgagtttcgg catctcttct
      181 ccttcgtagc gaaatcgggc tctgaccccg attctcttct tttatgatag cgacaggagg
      241 aggacccggc ttgaaggcgc cgtcgtttgc ctatgcgaaa ccgcgcacgc tggccgaggc
      301 gttcgagctc gtcgagcgcc cgggcgccaa gatcctcgcc ggcggccaga gcctcattcc
      361 ggcgctcaac atgcgccttt cggcgcccga gctcctcgtc gatatcacgg gactcgcttc
      421 gcttagaggc gtcacgctcg agcaaggcgt agtgcgcatc ggcgcgctca ccacgcatgc
      481 cggcatcgag cgctccgcgg aaatcccgaa gcatgtgccg cttcttgcgg aggcggtgcg
      541 gcacatcgct caccccgcga tccgcaaccg cggcacgctg ggcggcagca tcgcgctcgc
      601 cgaccccgcc gcggagtatc ccgcgtgcct ggtagcgctg gaagccacca tcgtgctgca
      661 gtccaaggcc ggcgagcgca tggtgcgggc ggaggagttc ttcaagggcc tattcgaggt
      721 cgacctcgct gtcggggagc tggtggccgc ggtagagatc ccggcgcagc tcaaggacga
      781 gcgctcggtg ttcctcgagc ttgcgcggcg ccacggcgat tacgcgatcg tcgggctagc
      841 cgcagtcacg aagctctccg gacgcaggct tgtctttttc gggctctcga acaaacccgt
      901 tcttgtgaag ccgagctcgc tcgaggaggt gaaggccgag ctgccgacgc ccgcggccga
      961 tctctacaac tctccttcga ccaagctgca cctcgcgaaa gtcctgctgg agcgcgcatg
     1021 gaacaggctc tgaccatcac cgtgacggtg aacggcacgc agcttacccg cagcgttgcc
     1081 gcgcgccagc atctggtcga tttcctgcgg gaagagctcg ggctcaccgg ctcgcatatc
     1141 ggctgcgagc acggcgtctg cggcgcgtgc acggtgcgcg tcaacggcga catcgtgcgc
     1201 ggctgcctga tgctcgcagt gcaggcgaat ggctgccgcg tggacacgat cgaaggactg
     1261 tcggattcca aggagctcgc gaagctgcag aaagcgttcc acgagaagaa cgcgctgcag
     1321 tgcggcttct gcactccggg aatgctgatg gccgcccagg acctcgtgcg catgaataag
     1381 aagttctccc gtgaagaaat acggagccac atctccggta attactgccg ctgcacgggc
     1441 taccaggcga tcgtcgacgc catcgacgag gtgatcaatg ggtaaggcag aactgccgct
     1501 ccacgcgccg ggcacgcagc tcgacaaggg ctatatcggc cagagcgtgc cgcgcgcgaa
     1561 cgcccagcgc ctgctccagg gccgcggcgc tttcaccgac gatctgcgct ttccgcgcct
     1621 ggcgcatgtg gcgttctttc gcagccccta cgcacacgca aggatcgcga agctggagtt
     1681 ccggaatgcg cttagcgcac ccggagtcat tgccgtggtc gatggcaggg cagtggcgga
     1741 atactgcaag ccctgggtcg ccgtgctcgc ccacctgaaa ggcatcaagt cgccgcccca
     1801 gcacgcgatc gccatcgagc gcgcgtgctg gcagggcgaa gcgtgcgccg ccgtggtcgc
     1861 cgaatcgcgt agccaggccg aagacgccgt agcgctgatc gaggccgaat gggaagagct
     1921 ccccgtcgtt gtcgacatgg aggccgcgct gcaaggaaat gtcgtcatcc acgaagagct
     1981 gaaagacaac gtctgcttca agcgcgagct ggacaccggc ggggtcgacg aggccttcgc
     2041 cagcgcagat atggtggtcg aggacacgtt ccacttcggc cgccacacgg gtgtctgccc
     2101 cgaggggcgc gcgatcctcg ccgactacaa cgccgccgag cactcgctca ccgtgtacca
     2161 cgcgacacag gcgccgcaca tgatgcagga catcttctcg cgccacctcg gcatcccgga
     2221 ggccaacgtg cgcgtgatcg cgaaggatgt cggcggctcc tacggcatca aggtgcacgt
     2281 gtacgcggac gagatggcca cggcggcgct ctccgtgatg ctgcgccggc cggtgaaatt
     2341 cgtcgccgac cggctcgagt catttctgtc cgatattcat gcacgcgaac accgtgtcaa
     2401 ggtcaggctg gcggtaaaga gcacgggcga aattctggcg ttcgacctgg acgatttgac
     2461 ggcgatcggc ccgtactcgg tgtacccgcg caccagcggc atcgagggca accaagtcgt
     2521 caacctcacc ggcggtccgt acaagcatca gaagtacaag gcgcgcatgc acgtcgtgtt
     2581 cacaaacaag aacgtgacgt gccagtaccg cgcggtcggc catccgatcg cggtcgcgct
     2641 gaccgaaggc atcgtggact tggcggcgca gaagctcggc atggatccgg ccgagttccg
     2701 caaaaagaac ctgatcccgg acgaggccta tccgtacacc tttccgtcgg gcatcaagtt
     2761 cgagaagctc tcgcaccaca aggcgctcga caagcttctc gctctcatga actaccacaa
     2821 gctccgtgag gagcagaagc agcttcgcga ccgctcggtt ttccgcggga tcggcctggc
     2881 ggcgatgatc gaggtgacca atccctcgcc cgccttctac ggcgtcggcg gcgcgcgcat
     2941 ctcggcgcag gacggggcga cgctgcgttt cgagcccgcc ggcatggtga ccgtgatgtg
     3001 cagcgtcacc gagcaagggc aggggaccga gggcatcttc gcgcagatcg cggcgagcgc
     3061 ggtcggcgtg tccatggaca aggtaagggt gatcaccggc gataccggcg tcacgcctta
     3121 cggcggcggc acctgggcat cgcgcggcgc cggcatcggc ggcgaagcgg tgctgcaggc
     3181 gggccgtgcg cttcgctcga acatgctcga cgtcgcttcg aggatcctca atctcgacaa
     3241 ggccgagctc gacatcgcga acaatgcggt cgtcagcagg ctgactcagg agaggaagct
     3301 cgatcttgcg gaactgggaa gaatcgccta cttccggccg gatacgttgc cgatcgactt
     3361 ccagtccgag ctcaccgtca cgcgtcacta cacgccgcgt cagtacggct tcacgttcac
     3421 caacggtatc caggcctcgc tggtggaact cgatccggac accggccttg tgaagctgct
     3481 caagcactgg tgcgtggagg attcgggcac gctgatcaac ccgatgctgg tcgacgagca
     3541 gatccggggc ggggtggtgc agggcatcgg cgctgcttta tatgaagagt gcctctatag
     3601 ccctgacggc cagctgctca acggcaacat ggccgattac ctcgtgccga tgtcgggcga
     3661 gatgcccgac atcgtgtgcg cgcacatcga gacgccgacg ctgcagtcac agcttggcgc
     3721 gaaaggcgtc ggcgaggccg ggaccgcggg tgcgcccgcc gcggtgatga acgccattaa
     3781 cgacgcgctc gccccgctca atgcgcgcgt cacccgcatg ccgttcacgc ccgaacgaat
     3841 cctgcaggcg ctgggtaagg tctaggctgc tacttgctga tagtcgaccg ccgagatatc
     3901 cgcgcgctcg ctcgcatgct ccagcgtcag gccacacaac ttgccggccg cgtccagatc
     3961 aatctgcgtg ttcttgtcga gatcgcgggt ttccgtcacg tccgcgcgcc ggaattccat
     4021 gtacagcgta tcggtttcgt ggaagtacct gatcttcgcg gttcaaatct tcgatcgaag
     4081 aaagcattat gcactgtctc gccgtcggga gcactacgct ttgcttagcg gtcggatgac
     4141 aacgaaaccg aaagatgcgc gcgttagctc cgccatctcg cattgggcgc cgcgcttcgt
     4201 ctccaatggg gtgctgctcg ccgacttcga ggacgtcacc gggtcgctcg agcgctggga
     4261 ggactggtgc gcggcctgga gcgcgcgcgc gaaagtgcat gaggatcttg gccgggaagc
     4321 gttgacgcag gaatttcgta tgactgc
//
LOCUS       DASUNT010000433         2118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1201015, whole genome shotgun sequence.
ACCESSION   DASUNT010000433 DASUNT010000000
VERSION     DASUNT010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201015"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VFX94_08790"
     CDS             <1..472
                     /locus_tag="VFX94_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768316.1"
                     /translation="KLVKRLSDLGFLFVLVAAVEAVYALTAILTPPGQIEAVTGWVLS
                     ADGKWLVKLLGLALASQAWVAWTLRNQPHLGVAKALALYQLGSATADWVMWIALADQN
                     VFSTTLGQLLVMVSIPTHYALGFLLVRAIRRASARQADSAPAGTFPPAVANPRA"
     gene            complement(647..>2118)
                     /locus_tag="VFX94_08795"
     CDS             complement(647..>2118)
                     /locus_tag="VFX94_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5768317.1"
                     /translation="EALRLLARAAAGPLDPLDRARLKFLHGQIEVDLSHGPAALPLLL
                     DAARQLEPLDVPLSRDAYLAALRAASVAGRLGPGMLEVARAALEAPRLPDERHAVDLL
                     VNGLAVRFTDGYAASAPALKRALAALREEGERKEVDVRWPWFARRVAGELFADDAWHY
                     LTTRSVQVARENGALAVLPLALNHRAHLCCLEGDLDGASALLDEADGIAVATGTEPLV
                     FGRLPLAGIRGIEAEAVVIFEAAVPAVIARGGEGVVLTFADHARAVLYNGLGRYEAAL
                     APAQEAANRDELLVSVWSLPELVEAATRCGQADVANAAIESLSERTRAAGTELALGIE
                     ARSRALLSEGALAERLYREAIDRLGRSRMAFELARAHLLYGEWLRRDRRRIDARDQLR
                     LARDAFTSMGAEAFAARAGRELLATGETARKRTAETRDDLTAQETQIAQFARDGLSNA
                     EIGARLFISPRTVEYHLHKVFLKLDITSREHLDRVLPGD"
BASE COUNT          315 a          731 c          715 g          357 t
ORIGIN      
        1 gaagctcgtg aagaggctct cggatctcgg tttcctattc gtcttggtcg ccgccgttga
       61 ggcggtctac gcgctcaccg ccatcctcac tcctcccggc cagatcgagg ccgtgaccgg
      121 ctgggttctg agcgcggacg gcaagtggct ggtgaagctg ctcggcctcg cgctcgcctc
      181 gcaggcctgg gtcgcctgga cgcttcgaaa ccaaccgcac ctcggcgtgg ccaaggcgct
      241 cgcgctctac caactcggca gcgccacggc cgactgggtg atgtggatcg cgctcgccga
      301 tcagaacgtg ttctcgacca ccctgggcca attgctggtg atggtctcga tcccaaccca
      361 ttacgcgctc ggctttctgc tcgtgcgagc gatccggcgt gcgtccgcgc gacaggccga
      421 ctccgctcct gcgggaacgt tcccgcccgc ggttgctaac ccgcgcgctt aggcggcgca
      481 gctcacccgt ctcatggagg aaggcccgtg accgcttggt cagccttgga ttccacgggt
      541 cggcgtcaac gccccagcgc tgccagcgag gggaccgccg gttcgaattc gcgcgaccga
      601 acagagagtt cttgcgagtt cgcatgcgca accgcgtcct ggggactcag tcgccgggaa
      661 gcacgcggtc caggtgctcg cgactggtga tgtccagctt gaggaacacc ttgtgcaggt
      721 ggtactcgac ggtgcggggg ctgatgaaga gacgcgcgcc gatctcggcg ttggagagcc
      781 cgtcgcgggc gaactgtgcg atctgcgtct cctgtgcggt gaggtcgtcc cgggtttcgg
      841 cggtgcgctt tcgagctgtt tcgcctgtcg ccagcagctc gcgtcccgcg cgtgcggcga
      901 acgcctcggc gcccatcgag gtgaacgcat cccgggcaag gcgtagctgg tcgcgagcgt
      961 cgattcggcg tctgtcccgt cgcagccatt cgccgtagag caggtgagcg cgagccagct
     1021 cgaacgccat gcgagatcgc ccgaggcgat cgatcgcctc cctgtagagc cgttctgcga
     1081 gcgcgccctc actcagcagc gctcgcgacc gtgcctcgat gccgagcgcc aactcggtgc
     1141 ccgccgcgcg tgtccgctct gataatgatt cgatcgcggc gttcgcgacg tcggcctggc
     1201 cgcagcgcgt cgcggcctcc acgagctcgg gcaacgacca caccgacacc agcagctcat
     1261 cccggttggc ggcttcttga gcgggcgcta gggcggcctc gtagcgaccg aggccgttgt
     1321 agaggactgc ccgtgcgtgg tctgcgaagg tgagcacaac tccctcgccg cctcgagcga
     1381 tcacagccgg cacagccgcc tcgaagataa caaccgcctc cgcttcgatt ccgcgaatgc
     1441 cggctaacgg aagtctcccg aagaccaatg gttcggtgcc tgtcgcgacc gcgatgccat
     1501 cggcctcgtc gagcagggcg ctcgctccat cgaggtcgcc ctccaagcag cacaggtgcg
     1561 cgcgatggtt gagcgcgagc gggaggacgg cgagggctcc gttctcacgt gcgacttgga
     1621 cgcttcgagt ggtgaggtag tgccaggcgt cgtcggcgaa cagctccccg gcaacgcgtc
     1681 gcgcgaacca cggccagcgg acatctactt ccttccgctc gccctcctcg cgaagcgcgg
     1741 cgagtgcacg tttcagcgcg ggcgcgctgg cggcgtaacc gtcggtgaac cgaaccgcca
     1801 gcccgttgac gagcaggtcg acggcgtgac gctcatccgg tagccgcggt gcctcgagag
     1861 ccgcgcgtgc gacctccagc atcccggggc cgagcctgcc ggcgacgctc gcggctcgta
     1921 gggcggccag gtacgcgtcg cgcgaaagtg gaacgtcaag cggttcgagc tgcctggccg
     1981 cgtcgagaag cagcggcagc gcagctgggc cgtggctcag atccacctcg atctggccgt
     2041 gcaggaactt cagcctggcg cgatccaacg ggtccagcgg cccggcggcc gccctcgcga
     2101 gcagccttag cgcttcct
//
LOCUS       DASUNT010000434         2515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_500179, whole genome shotgun sequence.
ACCESSION   DASUNT010000434 DASUNT010000000
VERSION     DASUNT010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500179"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VFX94_08800"
     CDS             <1..626
                     /locus_tag="VFX94_08800"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5768318.1"
                     /translation="RRAYLSLDARSREEVGALLGKLAAPQQARLVGALQTVEKLLASD
                     PSNGETIALRGHRPGDMGWVIHAHGRLYFEEYGWDERFEALVAGIAADFVKNFDIERE
                     RCWIAEMNGEPVGSVFVVKQSRTVAKLRLLIVEPKARGRGLGKRLVEECIAFARARGY
                     RKLVLWTQSDLAAARKIYARTGFQRVKQQPHREFGVRLLGEYWELEL"
     gene            623..1171
                     /locus_tag="VFX94_08805"
     CDS             623..1171
                     /locus_tag="VFX94_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX5768319.1"
                     /translation="MRLEEFVAELRRTRSECKDEREMLSRVRRLAPKLALSEGWVQRK
                     HYEADAEQGFGIHVLHEEADHTLAVFAVSWLPARGTPPHDHGTWAVIVGVDGAERNVF
                     WERADDRSRPGYAELRRIGDKVADRGDVVAMPTGAIHSVLNESDRVTLSLHVYGKHVN
                     HTERSQFDPARRTEAAFKLKLA"
     gene            complement(1152..2267)
                     /locus_tag="VFX94_08810"
     CDS             complement(1152..2267)
                     /locus_tag="VFX94_08810"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5768320.1"
                     /translation="MAEQERRRNLVSIEQEELRGISRTVAEIHWLLLILVLVYLVLGG
                     ERPDADAVSDAAVSAGLFFYAALVMCFRYANFYKRETRWKIAIETLGMISFITWVLWF
                     TERLEGPLINLYLLPVITSALTLGKLPTLSNVGLIAACYIFLGSASPDEMMSLRFAAG
                     FFAELAPVLLVAYITTMFSADIRYGLQHAKLLAETDDLTGLYNTRGFAIAANRLFGQA
                     TRYGRPASVLMIDSDNLKHVNDAHGHDAGNRLLRQLANAVQAELRATDVPARYGGDEF
                     IVLLPETPPKGSMDVAERIRNAIAARPLAIDGHQLTATVSIGVACYPEDGRTLDALAA
                     RADRALYQAKQEGRNRVVRYIAAGEPAVPAPAKPAST"
     gene            2306..>2515
                     /locus_tag="VFX94_08815"
     CDS             2306..>2515
                     /locus_tag="VFX94_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5768321.1"
                     /translation="MEFSAKGLRVLVTAGAAGIGRVIARTFVDHGARVHICDVDEKAL
                     ASTKKQLPTVTQTLADVAKLEDVDRL"
BASE COUNT          430 a          792 c          905 g          388 t
ORIGIN      
        1 ggcgccgcgc gtacctgagc ctggacgcgc gctcacgcga ggaagttggc gcactgctcg
       61 gcaagctggc agcgccgcag caggcgcggc tggtcggtgc gctgcagacc gttgaaaagc
      121 tcttggcgag cgatccgagc aacggggaaa caatcgctct gcgcggccat cggcccggcg
      181 acatgggatg ggtgatccat gcccacggcc ggctctattt cgaagaatac gggtgggacg
      241 agcgcttcga ggccctggtg gccgggatcg cggcggattt cgtgaagaac ttcgacattg
      301 aacgcgagcg ctgctggatc gccgagatga acggcgagcc ggtgggctcg gtattcgtcg
      361 tcaagcagtc gcggacggtc gcaaagctgc gattgctcat cgtggagccg aaagcccggg
      421 gccgcgggct ggggaagcgc ctggtggagg agtgcatcgc tttcgcacgc gccaggggct
      481 accgcaagct cgtgctctgg acgcagtcgg acctcgccgc ggcgcgaaaa atatatgcca
      541 gaaccggatt ccagcgcgtg aagcagcagc cgcaccgcga gttcggcgtt agactgctcg
      601 gcgagtactg ggagctcgag ctgtgagatt ggaagagttc gtcgccgagc tgcgccgcac
      661 gcgaagcgaa tgcaaggacg agcgggagat gctctcgcgc gtgcgccggc tcgcgccgaa
      721 gctcgcgctc tccgaaggct gggtacagcg caagcactac gaggcggacg cggagcaggg
      781 cttcggcatc catgtcctgc acgaggaggc cgaccacacg cttgcggtgt tcgcggtgag
      841 ctggctgcct gcgcgcggca cgccgccgca cgaccacggc acctgggcgg tcatcgtagg
      901 ggtggacggc gcggagagga acgtgttctg ggagcgcgcc gacgaccgct cgcgccccgg
      961 ctacgccgag ctacgccgga tcggcgacaa ggtcgcggac cgcggcgacg tggtcgccat
     1021 gcccacgggc gcgattcaca gcgtgctgaa cgaatcggac cgcgtgacgc tctcgctcca
     1081 cgtctacggc aagcacgtga accacaccga gcgctcgcag ttcgaccccg cgcggcgcac
     1141 cgaggcggcg ttcaagttga agctggctta gccggcgccg gcacggcggg ctcgccggcg
     1201 gcgatgtagc gcaccacgcg gtttcttccc tcctgcttcg cctggtagag cgcgcggtcg
     1261 gcgcgcgcgg cgagcgcatc gagcgtacgg ccgtcctcgg gatagcaggc gacgccgatg
     1321 ctgacggtgg cggtcagctg gtggccgtcg atggcgaggg ggcgcgcggc gatggcgttg
     1381 cggatgcgct cggccacgtc catggagcct ttcggcggcg tctcgggcag gagcacgatg
     1441 aactcgtcgc cgccgtagcg cgcgggcacg tcggtcgcgc gcagttccgc ctgcaccgcg
     1501 ttcgccagct ggcgcagcag ccggttgccg gcatcgtggc cgtgcgcgtc gttgacgtgc
     1561 ttgaggttat cagagtcgat catcagcacg ctcgcgggtc gaccgtagcg cgtcgcctgc
     1621 ccgaacaggc ggttagcggc aatcgcgaag ccgcgggtgt tgtagagccc ggtgaggtcg
     1681 tcggtttccg caagcagctt ggcgtgctgc aggccgtagc ggatgtcggc cgagaacatc
     1741 gtggtgatgt aggcgacgag cagcaccggc gcgagctcgg cgaagaaccc ggccgcgaag
     1801 cgcagcgaca tcatttcatc cggggatgcg ctgccgagga agatgtagca ggcggcgatc
     1861 aggccgacat tgctcagcgt gggcagcttg ccgagcgtga gcgcggaggt gatgaccggc
     1921 aggagataca ggttgatgag cggcccttcc aggcgctcgg tgaaccagag cacccaggtg
     1981 atgaagctga tcatgcccag cgtctcgatc gcgatcttcc agcgcgtctc gcgcttgtag
     2041 aagttcgcgt agcggaagca catcaccagc gcggcgtaga agaaaagccc cgccgagacc
     2101 gccgcgtccg acacggcatc ggcatcgggc cgctcgccgc ctaacaccag atagacaagc
     2161 accaggatga gcagcagcca gtggatctcc gccacggtgc gcgagatgcc ccgcagctct
     2221 tcctgctcga tcgaaaccag attgcgcctg cgttcttgtt ctgccaccgc tttccccgct
     2281 aacccggcgt gtattatgcg gccgcatgga attctctgcc aaaggcctgc gggtgctcgt
     2341 cacggcgggg gcggccggca tcggccgcgt gatcgcccgc acgttcgtcg accacggcgc
     2401 gcgggtccat atctgcgacg ttgatgaaaa agcccttgcc agcacgaaga agcagctgcc
     2461 gacggtgacg cagacgctcg ccgacgtggc gaagctcgag gacgtcgatc gcctg
//
LOCUS       DASUNT010000435         2853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_674848, whole genome shotgun sequence.
ACCESSION   DASUNT010000435 DASUNT010000000
VERSION     DASUNT010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674848"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="VFX94_08820"
     CDS             <1..1145
                     /locus_tag="VFX94_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5768322.1"
                     /translation="VVFKLKWPESSFLLNLSSPWNFIYRADTLAKDPKWYEKNINGTG
                     PFTFVEHVKGSHWVGKKNPNYWDKGKPYLDGFRAIFMSSSSAQVAAVRGERAHIQFRG
                     FTPKDRDTLKEALGNKITVQESPWDCILLVAINHEKKPFDDKRVRRALTLALDRHGGS
                     KALSQIAIVKEVAGVQVPGTPFATPSEELNKLAGYWTDAEKSRAEARRLLKEAGVPEG
                     FQFTFKNRGIPMPYEPLGVWMIDQWRRVGLNVKQEVIEAAAYFGVIRKGDFEAAMDFQ
                     CGYIVEPDLDMYKFLSTDRNPANYGRYKDPVLDQLYDQQSRETNVEKRKQIVRQFEKR
                     LLDEEAHYLMTLQWHRIIPHSSKVKGWTITPSHYLNNTLDTVWLSE"
     gene            1159..2115
                     /locus_tag="VFX94_08825"
     CDS             1159..2115
                     /locus_tag="VFX94_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5768323.1"
                     /translation="MGAYILKRLLAMIPTLLGVAVLIFILLRVVPGDVVEARFLTQGS
                     QFQSQELMDIERKKLGLDQPLWKQFTTWMAGLARLDFGLSMWTGSPITEEIKLRFALS
                     LQLAIMASTIATLLAIPLGILAAVKQDTWVDYAVRVFSIAGLATPSFWLGILMILTML
                     IFFKWIPPMVFTPFWVDPWQNLLQLIWPALAVGYRYSAVGTRMMRSAMLEVLREDYIR
                     TARAKGLWLKLILSRHAVKNAILPVLTIISLEFAFLLGGLVVTEQVFNLNGLGMLFVE
                     AIARRDYTLTQALVMLVATVFLLVNFVVDIAYAWLDPRIRYR"
     gene            2081..>2853
                     /locus_tag="VFX94_08830"
     CDS             2081..>2853
                     /locus_tag="VFX94_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5768324.1"
                     /translation="MPGSIPASATAEELARLDARRAGWFGVAVDFTRRRPLGAIGAAI
                     ILLMVVMAATAGLIAPYNPLENDYAAMLSAPNAEHWLGTDAHGRDVLTRIIYGSRTAL
                     LVGLGASLLGATFGSLIGVASAYFGGRVDLIVQRIMDVFFAFPVIILALAVVAVLGTG
                     PENVILAIAAPMVPRCARVVRASALAVREMPYVDAARAGGYGHGRIILRHMLPNVMAP
                     ILIMATAFLGEAILLEASLSFLGLGVQEPTAAWGLMLRGA"
BASE COUNT          467 a          940 c          937 g          509 t
ORIGIN      
        1 ccgtggtgtt caagctgaag tggccggagt cgtcgttcct gctgaacctg agctcgccgt
       61 ggaacttcat ctatcgtgcg gatacgctcg ccaaggatcc caagtggtac gagaagaaca
      121 tcaacgggac cgggccgttc acgttcgtcg agcacgtcaa aggctcgcac tgggtcggca
      181 agaagaaccc caactactgg gacaagggca agccctacct cgacggcttc cgcgccatct
      241 tcatgagctc ctcgtcggcg caggtcgccg cggtgcgcgg cgagcgcgcg cacatccagt
      301 tccgcggctt tacgcccaag gaccgcgaca cgctcaagga agcgctcggc aacaagatca
      361 cggtgcagga gtcgccgtgg gattgcatcc tgctagtggc gatcaaccac gagaagaagc
      421 ccttcgatga caagcgtgtg cgcagggcgc tgacgctcgc gctcgaccgc catggcggct
      481 ccaaggcgct gtcgcagatc gcgatcgtca aggaagtggc cggcgtgcag gtgccgggaa
      541 cgcctttcgc gacaccgtcg gaggagctga acaagctcgc cggctactgg accgacgccg
      601 agaagtcgcg cgccgaggcg cgccggctgc tgaaggaagc cggcgtcccc gagggcttcc
      661 agttcacgtt caagaaccgc ggcatcccga tgccgtacga gccgctgggc gtttggatga
      721 tcgaccagtg gcgccgcgtc ggcctgaacg tcaagcagga ggtgatcgag gcggccgcgt
      781 acttcggcgt catccgcaag ggcgacttcg aggcggccat ggacttccag tgcggctaca
      841 tcgtcgagcc cgacctcgac atgtacaagt tcctctcgac cgaccgcaac cccgcgaact
      901 acgggcgcta caaggacccg gtgctcgacc agctctacga ccagcagagc cgcgagacga
      961 acgtcgagaa gcgcaagcag atcgtgcgcc agttcgagaa gcggctgctc gacgaggagg
     1021 cgcactacct gatgacgctg cagtggcacc gcatcatccc gcactcgtcg aaggtgaaag
     1081 gctggacgat cacgccgagc cactacctca acaacacgct cgacacggtc tggctgtccg
     1141 agtgaggcga tagcggagat gggcgcctac atcctcaagc ggctgttggc gatgatccca
     1201 acgctgcttg gggttgcggt gctcatcttc atcctgctgc gcgtcgttcc cggcgacgtg
     1261 gtcgaggccc gcttcctgac acaaggaagc cagttccagt cgcaggagct gatggacatc
     1321 gagcgcaaga agctcggcct cgaccagccc ctgtggaagc agttcaccac ctggatggcg
     1381 gggctcgcgc ggctcgactt cgggctttcg atgtggaccg gctcgccgat caccgaggag
     1441 atcaagctgc gcttcgcgct ttcgctgcag cttgcgatca tggcgagcac catcgccacg
     1501 ctgctcgcga ttccgctcgg catcctggcc gcagtcaagc aggacacctg ggtggactac
     1561 gcggtgcgcg tgttctcgat cgccgggctc gccacgccgt cgttctggct cggcatcctc
     1621 atgatcctga cgatgctgat cttcttcaaa tggattccgc cgatggtgtt cacgcccttc
     1681 tgggtggatc cgtggcagaa cctgctgcag ctcatctggc cggcgctggc ggtgggctac
     1741 cgctattccg ccgtgggcac gcgcatgatg cgctcggcga tgctcgaggt gctgcgcgag
     1801 gactacatcc gcacggcgcg cgcgaagggc ttgtggctga agctgatcct ctcgcgccac
     1861 gccgtcaaga acgcgatcct gccggtgctc accatcatca gcctggagtt cgcgttcctc
     1921 ctcggcggcc tggtggtgac cgagcaggtt ttcaacctga acgggctggg aatgctgttc
     1981 gtcgaagcca tcgcgcggcg cgactacacg ctcacgcaag ccctggtgat gctggtggcg
     2041 acggtcttcc tgctggtcaa cttcgtggtc gatatcgcct atgcctggct cgatccccgc
     2101 atccgctacc gctgaggaac tggcccggct ggacgcgcgg cgcgccggct ggttcggcgt
     2161 ggccgtggat ttcacccgcc ggcgcccgct cggcgcgatc ggcgccgcga tcatcctgct
     2221 catggtggtg atggcggcga ccgccgggtt gatcgccccg tacaacccgc tcgagaacga
     2281 ctacgctgcg atgctgtcgg cgccgaacgc cgagcactgg ctcggcaccg atgcgcacgg
     2341 ccgcgacgtg ctcacgcgaa tcatctacgg ctcgcgcaca gcgctgctgg tcgggctggg
     2401 agcgtccctt ctgggagcga cgttcggctc gctgatcggg gtggcgagcg cgtacttcgg
     2461 cggccgcgtg gacctgatcg tgcagcggat catggacgtg ttcttcgcct tcccggtcat
     2521 tatcctcgcc ctcgccgtgg tggcggtgct cggaaccggc cccgagaacg taatcctcgc
     2581 tatcgctgcg cccatggtgc cgcgttgcgc gcgcgtggtg cgcgccagcg cgctcgcggt
     2641 gcgcgagatg ccgtacgtcg acgccgcgcg cgccgggggc tatggtcacg gtcgcatcat
     2701 cctgcgccac atgctgccca acgtgatggc gccgatcttg atcatggcca ccgcgtttct
     2761 cggcgaggcg atcctgctcg aggcctcgct ttcgttcctc ggcctgggcg tgcaggagcc
     2821 aaccgcagcg tgggggctca tgctgcgcgg cgc
//
LOCUS       DASUNT010000436         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_762394, whole genome shotgun sequence.
ACCESSION   DASUNT010000436 DASUNT010000000
VERSION     DASUNT010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762394"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFX94_08835"
     CDS             complement(<1..200)
                     /locus_tag="VFX94_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH dehydrogenase"
                     /protein_id="HEX5768325.1"
                     /translation="MKRENVIGRANVEDTPELEAYYRALEGEELGALWNVANEIEPWF
                     PQPKSVPIIWKWQRVEPFVRRAP"
     gene            complement(210..896)
                     /locus_tag="VFX94_08840"
     CDS             complement(210..896)
                     /locus_tag="VFX94_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5768326.1"
                     /translation="MLEVRNLETAYGRSQVLFGVSLEVGASQVVSLLGRNGMGKTTTV
                     RSIMGIARPKAGTITFDGKALNGLPPYRIAQAGFGLVPEGRQVFPNLTVRENLLATAR
                     KGPWTLERVLNLFRNLRERQGHYGNQLSGGEQQMLAIGRALMTNPRLLVLDEATEGLA
                     PLVRGEIYRSIERLKGDGLSILVIDKDVKSLTQVADRHYVLEKGRVVWSGSSAELAAD
                     QNVQHRYLGV"
     gene            complement(896..1627)
                     /locus_tag="VFX94_08845"
     CDS             complement(896..1627)
                     /locus_tag="VFX94_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5768327.1"
                     /translation="MLEVSGLTKTFGALRACDGIDFEVREGETHAVIGPNGAGKTTFI
                     SQLAGNLMPDAGTVRFAGEDITRLPAHRRARKGLARSFQITSVFLDFSALHNVALAVQ
                     SLSGRTGLWRPASADPALIEPARRLLEQVGLGARCDVPAVSMSHGEHRQLEVAMALAT
                     EPRLLLLDEPMAGMGVDESQRMIGLLGRLKQDKTIVLVEHDMDAVFRLADRISVLVYG
                     RVIATGVPDEIRANKEVRKAYLGEA"
     gene            complement(1620..>2438)
                     /locus_tag="VFX94_08850"
     CDS             complement(1620..>2438)
                     /locus_tag="VFX94_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5768328.1"
                     /translation="GYGGLVSFGHAAYLGIGAYAVGILAFYGITSGWLQWAVAIGASA
                     LVALAIGAVSIRTSGIYFIMITLAFTQMLYYLGISLEEFGGDDGMRLKAKSQFSRLVD
                     LNEPITFYYLVLVLMLACVYVVSRIVNSRFGMVLRAAKSNEARTRAIGLSPYPYRLAA
                     FVMAGAMCGLAGALYANHTNYIVPGLMSWQHSGDLMFMVILGGMASTAGPVLGAFAFI
                     FTEEFLKVLLNWIAPIFGKRDWAEYWAVLMGPLLVLSVIFFRRGLAGIFYKPDA"
BASE COUNT          412 a          789 c          841 g          396 t
ORIGIN      
        1 ggcgcgcggc gaacgaacgg ttcgacgcgc tgccacttcc agatgatcgg caccgacttc
       61 ggttgtggga accacggctc aatctcgttc gcgacgttcc acagcgcgcc cagctcttcg
      121 ccctcgagcg cgcggtagta cgcctcgagc tccggcgtgt cctcgacgtt ggcgcgtccg
      181 atgacgttct cgcgcttcat ccgaaaagtc tacaccccga ggtagcgatg ctggacgttc
      241 tggtctgccg cgagctcggc cgagctgccg ctccacacga cgcggccttt ttcgagcacg
      301 tagtggcggt ccgcgacctg ggtgagcgac ttgacgtcct tgtcgatgac gaggatggag
      361 agcccgtcgc ccttcaggcg ctcgatcgag cgatagatct ctccgcggac gagcggcgcc
      421 aggccctcgg tggcttcatc gaggacgagg aggcgggggt tggtcatgag cgcgcggccg
      481 atggcgagca tctgctgctc tccgccggag agctggttgc cgtagtggcc ctggcgctcg
      541 cgcaggttgc gaaagagatt cagcacgcgc tcgagcgtcc agggaccctt gcgcgccgtg
      601 gcgagcaagt tttcgcgcac cgtcaagttg gggaatacct gccggccttc gggaacgagg
      661 ccgaagccag cctgggcgat gcggtagggc ggcagcccgt tcagcgcttt gccgtcgaac
      721 gtgatcgtgc ctgccttcgg ccgcgcaatc cccatgatcg agcgcacggt ggtggtcttg
      781 cccatgccgt tgcggccgag caggctcacc acctggctcg cgccgacttc gagactcacg
      841 ccaaagagga cctggctgcg gccataggcg gtctcgaggt tgcgaacttc gagcatcagg
      901 cctcgccgag atacgctttg cgtacttctt tgttcgcgcg aatctcgtcg ggcacgccgg
      961 tcgcgatgac gcgcccgtag accagcaccg agatgcggtc ggcgagccgg aacaccgcgt
     1021 ccatgtcgtg ctcgaccagg acgatggtct tgtcctgctt gaggcgtccc agcaggccga
     1081 tcatgcgctg ggattcgtcg acgcccatgc cggccatcgg ctcgtccagc aggagcaggc
     1141 gcggctcggt ggcgagcgcc atggccacct cgagctggcg gtgctccccg tgcgacatgc
     1201 tcaccgccgg cacgtcgcag cgcgcgccca agccgacctg ctcgagcagg cgccgcgccg
     1261 gctcgatgag cgcggggtcg gcgctcgccg ggcgccataa gcccgtgcgc ccgctgagcg
     1321 attgcaccgc gagcgccacg ttgtggagcg cgctgaaatc gagaaagacg ctggtgatct
     1381 ggaacgagcg cgcgagtccc ttgcgcgcgc gccggtgcgc gggcaggcgc gtgatgtcct
     1441 cgccggcgaa gcgcaccgtg ccggcgtcgg gcatgaggtt gccggcgagc tggctgatga
     1501 acgtggtctt gccggcgccg ttcggcccga tgacggcgtg cgtctcgcct tcgcgcacct
     1561 cgaaatcgat gccgtcgcac gcgcgcagcg cgccgaaggt cttcgtcagc ccgctgactt
     1621 caagcatcgg gcttgtagaa gatccccgcc agcccgcggc ggaagaagat taccgaaagc
     1681 acgaggagcg ggcccatgag caccgcccag tattccgccc agtcgcgctt gccgaagatc
     1741 ggcgcgatcc agttgagcag caccttgagg aattcctccg tgaagatgaa ggcgaaggcg
     1801 ccaagcacgg ggccagcggt actcgccatg ccgcccagga ttaccatgaa catgaggtcg
     1861 cccgagtgct gccagctcat gaggcccggc acgatgtagt tggtgtggtt cgcgtagagc
     1921 gcgcccgcga gcccgcacat cgcacccgcc atgacgaagg cggccaggcg gtaagggtag
     1981 ggcgacaagc ctatggcgcg cgtgcgcgcc tcgttcgact tggctgcgcg cagcaccatg
     2041 ccgaagcgcg agttcacgat tcgggagacg acgtacacgc aggcgagcat gaggacgagc
     2101 acgaggtaat aaaacgtgat cggctcgttc aggtcgacga gtcgcgagaa ctggcttttg
     2161 gccttgaggc gcataccgtc gtcgccgccg aactcctcca gcgagatgcc gaggtagtag
     2221 agcatctgcg tgaaggcgag tgtgatcatg atgaagtaga tgccgctcgt gcggatagag
     2281 accgcgccga tggcgagggc gacgagcgcg gaggcgccga tcgccaccgc ccactgcagc
     2341 cagccgctcg tgattccgta gaaggcgagg atgcccaccg cataggcgcc gatgccgagg
     2401 taagcggcgt ggccgaacga gaccaggccg ccgtagcc
//
LOCUS       DASUNT010000437         2158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_850313, whole genome shotgun sequence.
ACCESSION   DASUNT010000437 DASUNT010000000
VERSION     DASUNT010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850313"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VFX94_08855"
     CDS             <1..703
                     /locus_tag="VFX94_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5768329.1"
                     /translation="GIRFLGVSRPGAYSTHLLVPDARYLVDSTGVDDAFAATLACSGL
                     TAYSAAAKLPQLRPQERVAVLGCGGLGLIGISVLRAKGVKHVVACDIDDAKLAAAAKL
                     GAKTTLNTKSPDATRELQGIAAAIDFVGSPATAALGIGALRKGGRYIICGLYGGELVH
                     PLPPIAQRAIGIVGSYVGSLQELKEVVSLAKRRKIRPAPVELRPADEAARALDDLKAG
                     RVLGRIVLDFENVAA"
     gene            717..>2158
                     /locus_tag="VFX94_08860"
     CDS             717..>2158
                     /locus_tag="VFX94_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX5768330.1"
                     /translation="MTAFAWNNPYPWPRKPLLAANVVATSQPLAAQAGLQMLADGGGA
                     VDAILSTAITLTLVEPVSNGIGSDAYAIVWDGERLHGLNASGRSPAGWTPEFFAGRSS
                     MPERGWNSVSVPGCVSAWVELHRRWGKLPFEKLFEKAIEYGRKGFLVSPTIAGQWEKQ
                     VPELKPQPGFADAFLPDGGPPRPGQLFRFTKHAETLERIRDTKGEAFYRGELAEKLEA
                     HAKKHGAAMRASDLAAHKSDWVEPLSMDYRGYTLHEIPPNGQGIVALMALGMLEHLDL
                     RSHPVDGADSVHLQIEAQKLAFADARRYVADIDYMTNVRPQALLDKEYLNSRAKLIDL
                     KRAQDFGHGAPPQSGTVYLAAADVSGLMVSFIQSNFMGFGSGVVVEGISLQNRGATFV
                     LQPGHPNCVGPRKRPYQTIIPGFVTRAGRPVMSFGVMGGTMQPQGHAQVMVRIADYGQ
                     SPQAACDGPRFRVVQGMDVSVESAFRPLVL"
BASE COUNT          351 a          713 c          744 g          350 t
ORIGIN      
        1 ggggatccgc tttctcggcg tgagccgccc gggcgcctat tcgacacact tgctggtacc
       61 ggacgccagg tatctggtcg attcgaccgg cgtcgacgac gcattcgcgg cgacgcttgc
      121 ctgctccggg ctcaccgcgt acagcgcggc ggccaagctg ccgcaactcc ggccgcagga
      181 gcgggtcgcg gtgctcggct gcggcgggct cggccttatc ggtatatcgg tgctgcgtgc
      241 caagggcgtg aaacacgtgg tcgcttgcga tatcgacgac gcgaagctcg cggccgcagc
      301 taagcttggg gccaagacga ctttgaacac gaaaagcccg gatgccacgc gcgagttgca
      361 gggcatcgcc gccgcaatcg atttcgtcgg cagcccggcc accgcggcgc tcggcatcgg
      421 cgcgttgcgc aagggcgggc gctacatcat ctgcggcctg tacggcggcg agctcgtcca
      481 tcctttgccg cccatcgccc agcgtgccat cggcatcgtg ggctcgtacg tcggaagctt
      541 gcaggagctg aaggaagtgg tctcgctcgc caagcggcgc aagatacgtc ccgcgccggt
      601 ggagctgcgc cccgcggacg aagcggcgcg cgcgctggac gacctgaagg ccggccgggt
      661 gctggggcgg attgtgcttg acttcgagaa cgtggcggcg tgaacaccaa tccccaatga
      721 cggctttcgc ctggaacaat ccctatccct ggccgcgcaa gccgctgctg gcggcgaacg
      781 tcgtcgccac ctcgcagccg ctcgcggcgc aggccgggct gcaaatgctc gccgatgggg
      841 gcggcgctgt ggacgcgatc ctgtccaccg cgatcacgct tacgctcgtc gagcccgtat
      901 cgaacggaat cggctcggac gcctatgcga tcgtctggga cggagagcgt cttcatgggc
      961 tgaacgcctc gggccgctcg ccggccgggt ggacgccgga gttcttcgcc ggtcgttcga
     1021 gcatgcccga gcgcggctgg aactcggtca gcgtgccggg gtgcgtctcc gcctgggtag
     1081 aactacaccg ccggtggggc aagctgccgt tcgaaaagct ctttgaaaag gcgatcgagt
     1141 acgggcgcaa gggcttcctc gtctcgccga ctatcgccgg gcagtgggag aagcaagttc
     1201 ccgagctcaa gccgcagccc ggcttcgccg acgccttcct gccggacggt ggccctcccc
     1261 gaccggggca gcttttccgg ttcaccaaac acgccgagac gctggagcgc atccgcgata
     1321 ccaagggcga agcgttttac cgcggcgagc tggcggagaa actggaggcg cacgcgaaaa
     1381 aacacggcgc cgcgatgcgc gcttccgatc tcgccgcgca caagtccgac tgggtcgagc
     1441 cgctttcgat ggactaccgc ggctacacgc tgcacgagat cccgccgaat ggccaaggca
     1501 tcgtcgcgct catggcgctc ggcatgctcg agcacctcga cttgcgctcg cacccggtgg
     1561 acggcgccga cagcgtgcac ttgcagatcg aggcgcagaa gttggcgttc gccgacgcgc
     1621 ggcgctacgt cgccgacatc gactacatga ctaacgtgcg gccgcaggcg ctgctcgaca
     1681 aggagtatct gaactcgaga gcgaagctga tcgaccttaa gcgcgcgcag gatttcggcc
     1741 acggcgcgcc gccgcagagc ggcacggtat acctcgcggc agccgacgtc tccgggctga
     1801 tggtgtcctt catccagtcg aacttcatgg gcttcggctc gggcgtggtg gtggagggta
     1861 tctcgctgca gaaccggggc gcgaccttcg tgctgcaacc gggccacccg aactgcgtcg
     1921 ggccgcgcaa gcggccgtac cagacgatca ttccgggctt cgtaacgcgc gcaggccggc
     1981 ccgtcatgag cttcggcgtc atgggcggca ccatgcagcc gcagggccat gcccaagtga
     2041 tggtgcgcat cgccgactac ggccagagtc cgcaagcggc gtgcgacggg ccgcgcttcc
     2101 gagttgtgca gggcatggac gtgagcgtgg aaagtgcctt tcggcccttg gtgctgga
//
LOCUS       DASUNT010000438         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_325795, whole genome shotgun sequence.
ACCESSION   DASUNT010000438 DASUNT010000000
VERSION     DASUNT010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325795"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VFX94_08865"
     CDS             complement(<1..543)
                     /locus_tag="VFX94_08865"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEX5768331.1"
                     /translation="MKHEAVGTVFIPGVVSLAASSRRTLKHGDSFAMFDEFGDVLEVQ
                     HSPQGVFHHDTRFLSRLHFMLEGRRPLMLSSTVQPDNVTLDVDLTNPDIFADGELRLA
                     KDTFHVARAKFLWQAGCYELFVVTSYAEQPTRLRLALEFDADFADLFEIRGYQRGRRG
                     AVRAEVRRPAEVRFVYDSLDG"
     gene            complement(540..1586)
                     /locus_tag="VFX94_08870"
     CDS             complement(540..1586)
                     /locus_tag="VFX94_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004527452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5768332.1"
                     /translation="MKIAQVAPLAESVPPRLYGGTERVVSYLTEELVRLGHEVTLFAS
                     GDSQTAARLVACAPRALRLDPEVTDALPHHLLMIERVRSRVHEFDVLHFHVEHVQLPL
                     FRPYAHKCVTTLHGRLDLPDLVPLYREFHEMPLVSISNTQRRPLAGANWVGTVYHGLP
                     DAVCPYNPAPRGQYLAFLGRISPEKGIEHAIEIARRAGQRLRIAAKIDRVDEAYWHSR
                     VKPLVSGPLIEFVGEVSEEDKPSFLGNASALLFPIDWPEPFGLVMIEAMSCGTPVIAW
                     PRGSVPEIVEPGVTGFITSSVDEAVAAAGAAVRLDRARVRQRFEESFSATRMALDYLA
                     LYRKVGVKKTELVP"
     gene            1654..>2024
                     /locus_tag="VFX94_08875"
     CDS             1654..>2024
                     /locus_tag="VFX94_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5768333.1"
                     /translation="MHIQAVKAAYRRYARIYDVIFGAVLQPGRRAVLEALNLKSGDRV
                     LEVGVGTGISLPLYPRNVRITGIDVSLEMLEKARARVERAKLSNVEALLEMDAETMAF
                     PDASFDKVVAMYVVSVVPRPG"
BASE COUNT          337 a          654 c          716 g          317 t
ORIGIN      
        1 gccgtcgagt gagtcgtaca cgaagcgaac ctcggccggg cggcgcacct cggcgcgcac
       61 cgcgccgcgc cggccgcgtt ggtagccgcg gatctcgaag agatcggcga agtcggcgtc
      121 gaactcgagc gcaagccgca gccgcgtggg ctgctcggcg tagctcgtca cgacgaagag
      181 ctcgtagcag cccgcttgcc aaaggaactt ggcgcgcgcg acgtggaacg tgtccttggc
      241 gagccgcagc tcgccgtcgg cgaagatgtc ggggttcgtg aggtcgacgt cgagcgtgac
      301 gttgtcgggc tgcacggtcg acgaaagcat cagcggccgg cgcccttcga gcatgaagtg
      361 caggcgagac aggaagcgcg tgtcgtgatg gaacaccccc tgggggctgt gctgcacctc
      421 gagcacgtcg ccgaactcgt caaacatggc gaagctgtcg ccgtgcttaa gcgtgcgccg
      481 gctcgacgcc gcaagcgaaa cgacgcccgg gatgaatacc gtgccgactg cctcgtgctt
      541 cacggaacga gctcggtctt cttcacgccc accttgcggt agagcgccag gtagtcgagc
      601 gccatgcgcg ttgcgctgaa gctttcctcg aagcgctggc gcacccgggc gcggtcgagg
      661 cgcacggcgg cgccggccgc ggcgaccgcc tcgtcgacgg agctcgtgat gaagccggtt
      721 acgccaggct cgacgatctc gggcaccgag ccgcgcggcc aggcgatcac cggcgtgccg
      781 caagacatcg cctcgatcat aaccaggccg aacggctcgg gccagtcgat ggggaagagg
      841 agcgccgagg cgttgccgag gaacgagggc ttgtcttcct cgctcacctc gccgacgaac
      901 tcgatcagcg ggccggagac gagcggcttc acgcggctat gccagtaggc ttcgtcgacg
      961 cggtcgatct tcgccgcgat gcgcagccgc tggccggcgc ggcgcgcgat ctcgattgcg
     1021 tgctcgattc ccttctcggg cgagatgcgg ccaaggaagg cgagatactg cccgcgcggc
     1081 gcggggttgt agggacacac ggcgtcgggc aggccgtggt aaacggtccc gacccagttc
     1141 gctcccgcga gcggccggcg ctgcgtgttc gagatcgaca ccagcggcat ctcgtggaac
     1201 tcccggtata gcggcacgag gtcgggcagg tcgagcctgc cgtgcagcgt ggtgacgcac
     1261 ttgtgcgcgt agggccggaa cagcggcagc tgcacgtgct cgacgtggaa gtgcagcacg
     1321 tcgaactcgt gcacgcggct acgcacgcgc tcgatcatga gcagatggtg gggcagggcg
     1381 tctgtcacct ccggatctag ccgcagcgcc cgcggggcgc aggccaccag gcgtgcggcg
     1441 gtctgcgaat ccccgcttgc gaagagcgtg acctcgtggc cgagccgcac cagttcctcg
     1501 gtcaggtagg acacgacccg ttcggtaccc ccgtacagcc tcggcggcac gctttccgcg
     1561 aggggtgcga cttgtgcgat tttcatattc ggtcgaaaca cgggtgcaat ccgcgtgccg
     1621 gaaatgccga ttgctggcag aatccgccaa ctcatgcaca ttcaagccgt caaggcggcc
     1681 taccggcgct atgcaaggat ctacgacgtc atcttcggcg ccgtgcttca gccgggccgc
     1741 cgggcggtgc tggaggcgct gaacctcaag tcgggcgacc gggtcctcga ggtcggcgtg
     1801 ggcaccggca tctcgcttcc gctctacccg cggaacgtgc gcatcaccgg gatcgacgtg
     1861 tcgctcgaga tgctggaaaa ggcacgcgcg cgcgttgagc gcgcgaagct ttccaacgtc
     1921 gaggcgctgc tcgagatgga cgccgaaacc atggccttcc ccgatgcgag cttcgacaag
     1981 gtcgtggcga tgtacgtcgt atcggtcgtg ccgcggccgg ggaa
//
LOCUS       DASUNT010000439         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_500531, whole genome shotgun sequence.
ACCESSION   DASUNT010000439 DASUNT010000000
VERSION     DASUNT010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500531"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VFX94_08880"
     CDS             <1..861
                     /locus_tag="VFX94_08880"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5768334.1"
                     /translation="VMVLADLDMPRLDGFGLLSRIRGCDDPRIRALPVVVISGNEDLE
                     AKQRARQLGANDFIGKSADAPEVLARLDSLLRLVRASQDATHDRRTGALTPHYLLTEG
                     RKHFGYARRHGSELSVMALRLDTYDDIVLAAGKEVADVVLARVAKLLMSKVRVEDSVA
                     RTAQGTFMVLAAATGAAQMLALAQRLHRELAEAKVTYQERPLRFAVSFGVASLPVDEV
                     GSIEELMRVALVRLHKQQAPPLAPRPAAALPAQLEAALRVLERADRAHLGPAAAELLK
                     RLKRIADALH"
     gene            866..>2166
                     /locus_tag="VFX94_08885"
     CDS             866..>2166
                     /locus_tag="VFX94_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5768335.1"
                     /translation="MPTYREFHRRSIEERERFWGEEAKLVHWQRPFQRVLEYARPPFA
                     RWFVRGETNLCYNAVDRHLASRGDQKALVWISTEVDQTRTFTFQELSREVNRVAAMMQ
                     SLGVKKGDRVIIYMPMIPEAAFAMLACARLGAIHSVVFGGFAAASLAARIDDARATLM
                     ITADGGSRMGKAVLYKSLVDESLKLAKHPPKKVLLFSRGLDPNMPVVKERDADWASLA
                     KDFPDPGVPCAWLESNEPSYILYTSGTTGRPKGVQRDVGGYAVALASSMKHIFCGEPG
                     ETMFTTSDIGWVVGHSYIIYGPLIAGMTTIMYEGVPIRPDAGIWWKIVQDYKVSVMFS
                     APTAIRVLKKQDPAYLKKYDVSSLKHLFLAGEPLDEPTHVWITEALGRPVIDHYWQTE
                     TGWPLLSAHPGVEQLKLKMGSPSFPVYGYDVQLLHEGTGKAV"
BASE COUNT          352 a          738 c          724 g          352 t
ORIGIN      
        1 gtcatggtgc tcgccgatct cgacatgccg cggctcgacg gattcgggct gctctcgcgc
       61 atccgcggct gcgacgaccc ccgcatccgc gccctgcccg tcgtggtgat ctcgggcaac
      121 gaggacctcg aagcgaagca gcgcgcgcgc cagctcggcg ccaacgattt catcggcaag
      181 tcggccgatg cgccggaagt cctcgcgcgc ctggacagcc tgcttcgcct ggtgcgcgcg
      241 agtcaggacg cgacccacga ccggcgtacg ggcgcgctga cgccccacta tctgctgacc
      301 gagggccgca agcatttcgg ctacgcgcgc cgccatggca gcgagctctc ggtcatggcg
      361 ctgcgcctgg acacctacga cgacatcgtg ctcgccgccg ggaaggaggt cgccgacgtg
      421 gtgctcgcgc gcgtcgcgaa gctcctgatg agcaaggtgc gagtcgaaga ctcggtggcg
      481 cgcaccgccc aggggacctt catggtgctc gccgcggcta ccggcgcggc gcagatgctc
      541 gccctggcgc agcgcctgca ccgcgagctc gccgaagcca aagtgaccta tcaggagcgg
      601 ccgctcaggt tcgcggtgag cttcggcgtg gcatcgctcc ccgtagacga ggttggctcc
      661 atcgaggagc tgatgcgggt cgcccttgtg cggttgcaca agcagcaggc gcccccgctt
      721 gcgccgcggc cagcggccgc tctgccggcg cagctcgagg cggccctgcg ggtgctggag
      781 cgcgcggacc gcgctcacct cgggcctgcc gccgctgagc tgctaaagag gctaaagcgc
      841 atcgccgacg cgctacacta attgcatgcc cacgtaccgc gaattccacc gccgctcgat
      901 cgaggagcgc gagcgcttct ggggcgagga agcgaagctc gtgcactggc agcggccgtt
      961 ccagagagtg ctcgaatatg cgcgcccgcc gttcgcccgc tggttcgtgc gcggcgagac
     1021 caacctctgc tacaacgcgg tcgaccgcca tctcgcgagc cgcggcgacc agaaggccct
     1081 ggtgtggatc tccaccgagg tcgaccagac caggacgttc actttccagg agttgtcccg
     1141 ggaagtgaac cgcgtggcag cgatgatgca gtccctcggc gtgaagaagg gcgaccgcgt
     1201 catcatctac atgcccatga ttcccgaggc ggcgttcgcg atgctcgcct gcgctcgcct
     1261 cggcgcaatc cattcggtgg tgttcggcgg cttcgccgcg gcgagccttg cggcgcgcat
     1321 cgacgacgcc cgcgcgacgc tgatgatcac cgcggacggc ggcagccgca tgggcaaggc
     1381 ggtgctctac aaatcgctgg tcgacgagtc gctcaagctc gccaagcatc cgccgaagaa
     1441 ggtgctgctc ttctcgcgcg gcctcgaccc gaacatgccg gtcgtgaaag aacgcgatgc
     1501 cgattgggct tcattggcaa aggattttcc cgatcctgga gttccctgtg cctggctcga
     1561 gtcgaacgag ccgtcctaca tcctctacac ctcgggcacc acggggcgcc cgaaaggagt
     1621 gcagcgcgac gtcggcggct acgccgtggc gctcgcctcg tcgatgaagc acatcttctg
     1681 cggcgagccg ggcgagacca tgttcacgac ctccgacatc ggctgggtgg tggggcactc
     1741 gtacatcatc tacggcccgc tcatcgccgg gatgacgacg atcatgtacg agggcgtgcc
     1801 gatccgcccg gacgcgggca tctggtggaa gatcgtgcag gactacaagg tctcggtgat
     1861 gttctcggcg ccgaccgcga tccgggtgct gaagaagcag gatcccgcgt acctgaagaa
     1921 gtacgatgtt tcttcgctca agcatctctt cctcgccggc gagccgctgg atgagccgac
     1981 ccacgtctgg attaccgagg ccctgggccg accggtgatc gaccactact ggcagaccga
     2041 gaccggctgg ccgctgctct cggcgcaccc cggggtggag cagctcaagc tcaagatggg
     2101 ctcgccgagc tttccggttt acggctacga cgtccagctg ctccacgagg gcacgggcaa
     2161 ggcggt
//
LOCUS       DASUNT010000440         4252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_587102, whole genome shotgun sequence.
ACCESSION   DASUNT010000440 DASUNT010000000
VERSION     DASUNT010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587102"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VFX94_08890"
     CDS             complement(<1..643)
                     /locus_tag="VFX94_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEX5768336.1"
                     /translation="MLVSEFDYELPGELIAQHPVAQRSESRLLHLDAPSGALRDMKFT
                     DFPQLLGPQDLVVLNDTRVVKARLAGRKASGGRIELFVERALGPREALALVRASHPPA
                     PGSELVIGDVRVRVERREGELYRVRFPEEVDSVLERHGAVPLPPYIRHAPQAQDAERY
                     QTVYAAVPGAVAAPTAGLHFDQDMLKKVEQTGASLARITLHVGFGTFQPVRAQT"
     gene            702..788
                     /locus_tag="VFX94_08895"
     tRNA            702..788
                     /locus_tag="VFX94_08895"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:736..738,aa:Leu,seq:caa)
     gene            886..1968
                     /locus_tag="VFX94_08900"
     CDS             886..1968
                     /locus_tag="VFX94_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX5768337.1"
                     /translation="MAKAQVRLLPSARNTVMVVDDQSTGRAILEQVIRSLDERVVVEG
                     FARPVDAVVWATRHVSDLVLVDYMMPDMDGIELVKRLRSLPGYEHVPIVMVTVHDDRK
                     VLYAALDAGITDFLTKPVDARECLARCRNLLTLRRQQLALEDRRRLLEHMVEDATREV
                     KEREKETLLRLARAGEFRDEETGNHLIRMSRYSRLIANALGIERDEAETIELAAPLHD
                     IGKIGIPDGILLKAHKLEQGEWEIMRRHPLIGHEILKGSGSKYVRMGALIALGHHEKY
                     DGSGYPNGLVGDHLPLCARIVAVADVYDALTSVRPYKSAWPSEQAFDYVRSQSGKHFD
                     PRMVDAFLSARKEVIQIQHEWSDGRI"
     gene            1955..>4252
                     /locus_tag="VFX94_08905"
     CDS             1955..>4252
                     /locus_tag="VFX94_08905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5768338.1"
                     /translation="MAGSSAAGIARTPGPIARLRARLKARPDTEHEQGILRLVITALG
                     ALYLLPDALAHQETWPLYVMLTHFVLSVIVFVRIAYSTEISPLRRVFAQFADVAAISW
                     YLGFFGEAGAFLFLLYIWVTLGSGFRFGARYLISELVMSVIGFGVVLYVNDFWHSHFE
                     LGVGLLVGMTALSLYVLSLVRRMFDALSRAEAANVAKRRFISMVSHELRTPLNAIIGM
                     ADLLRDTQLNREQADMLQTLRSSSRVMLGLIEDVLDFSKIEAGKVLLEKTDFDLHALV
                     NSTCRIVSAQAAVKGVDFVVSIMPEVPPAVRGDPHHLRQVLINLAGNAVKFTDRGSVT
                     VHVSVQAETETSVRLKFSLRDTGIGIAPEAQAKIFESFTQADESTTRRFGGSGLGTTI
                     AKQLVQLMGGRIGLESAIGLGSTFWVEIDLEKQPEQAGAAAGDLAGARVLLVGFPARE
                     RAALEAALAGWGAAPTAVANVEEGVARLVADISLAKPYHSALLYASGENRELAQRFRR
                     TAPNPAPPTILAVPREADVRRFEVLSAGFASVLELPFDKRRLFNVLHSVAAGEEVHEG
                     VVRLQDYARRSAAIKVRVLVADDNATNREVIGKILERSGHAITLVNDGEQTLDALERE
                     PHDIVILDRNMPGMGGMEALQAIRLMTRSRERLPVIMLSADVTPEVKREALEAGADSF
                     LPKPVAALRLLDEVERLAGKPEVQRKEAPAAAVPREAPSAPPIVNAETLGHLEELGSS
                     LAFVERLINVFVSDSSGILERIEKVL"
BASE COUNT          683 a         1433 c         1464 g          672 t
ORIGIN      
        1 ccgtttgtgc gcgcaccggc tggaaagtgc cgaagccgac gtgcagggtg atccgggcga
       61 gggacgcgcc cgtttgctct accttcttca gcatgtcctg gtcgaaatgc agccccgcgg
      121 tgggcgcggc gactgcgccc ggtacggcgg cgtacacggt ctggtagcgc tcggcgtctt
      181 gcgcctgcgg cgcgtgccgg atgtacggtg gcagcggcac ggcgccatgg cgctcgagga
      241 ccgagtcgac ctcctcgggg aagcgcacgc ggtagagctc gccttcgcgg cgctcgacgc
      301 gcactcgaac atcgccgatc acgagctcgg agcccggcgc gggcggatgg ctggcgcgga
      361 caagcgcgag cgcctcgcgc gggccgagcg cccgctcgac gaaaagctcg atcctgccgc
      421 cgctcgcctt gcggcccgcg agacgcgcct tcaccacgcg ggtgtcgttc aggacgacca
      481 ggtcctgggg cccgaggagt tgcgggaaat cggtgaattt catatcacgc agcgcccccg
      541 agggggcgtc gaggtggagc aggcggcttt cgctgcgctg cgcaacggga tgctgggcaa
      601 tgagttcgcc gggcagctcg tagtcgaact cggagaccag cattgacgcg aaagtatact
      661 tttcgcgcca agggctattc ttcgggcgag ttgccttcct ggccggaatg gcggaaccgg
      721 tagacgcagc ggactcaaaa tccgccgccc gcgagggcgt gagagttcga gtctctcttc
      781 cggcaccaca cagcgttaaa gaatgaccgc caaaacaacc ggttcgctgg acagggcggg
      841 gcggcggagg gtagtcttcc gagacaaaac accccgggga gggtcatggc caaggcacaa
      901 gtgcggctct tgccttcggc acgaaacacg gtgatggtcg tcgacgatca gtcgactggc
      961 cgtgccattc tcgagcaggt gatacgaagc ctcgacgaac gcgtggtggt cgagggcttc
     1021 gcgcgtccgg tggatgcagt ggtctgggcg acgcgccacg tgtcggacct cgtgctcgtc
     1081 gactacatga tgcccgacat ggacggcatc gagctcgtga agcgcctgcg ctcgctcccc
     1141 ggctacgagc acgtgccgat cgtgatggtc acggtgcacg acgatcgcaa ggtgctctac
     1201 gccgcgctcg acgcgggcat caccgacttc ctcaccaagc cggtcgacgc gagggagtgc
     1261 ctcgcgcgct gcaggaacct gctcacgttg cgccgccagc agctcgcgct cgaagaccgc
     1321 cgccgcctgc tcgagcacat ggtcgaggac gcgacgcgcg aggtgaagga gcgcgagaag
     1381 gagacgctgc tcaggctcgc gcgcgcgggc gaattccgcg acgaggagac cgggaaccac
     1441 ctgatccgca tgtcgcgtta ttcgcgtctc atcgccaacg cgctcggcat cgagcgcgac
     1501 gaagcggaga ccatcgagct cgccgcgccg ctgcacgata tcggcaagat cggcatcccg
     1561 gacgggatcc tgctcaaggc gcacaagctc gagcaaggcg aatgggagat catgcgtcgc
     1621 catccgctga tcggccacga gatcctgaag ggcagcgggt cgaaatacgt gcgcatgggg
     1681 gcgctgatcg cgctcggcca ccacgaaaag tacgacggct cggggtaccc caacggcctc
     1741 gtcggcgacc acctgccgct ctgcgcgcgc atcgtcgccg tcgccgacgt gtacgacgcg
     1801 ctcacctccg tgcgcccgta caagagcgcc tggccgagcg agcaggcgtt cgactacgtg
     1861 cgctcgcagt cgggcaagca cttcgacccg cgcatggtgg acgccttcct ttcggcacgc
     1921 aaggaagtga tccagatcca gcacgaatgg tctgatggcc ggatctagcg ccgccggcat
     1981 tgcgcggact cccgggccga tcgcgcgcct gcgcgcgcgc ctgaaggcgc gccccgacac
     2041 cgagcacgag cagggcatcc tgcggctggt gatcaccgcg ctcggcgcgc tctatcttct
     2101 gcccgatgcg ctcgcgcacc aggaaacttg gccgctgtac gtcatgctca cgcatttcgt
     2161 gctgtccgtg atcgtcttcg tgcgcatcgc gtactcgacc gagatctcgc cgctgcggcg
     2221 ggtgttcgcg cagttcgccg acgtcgccgc gatcagctgg tatctcggct tcttcggcga
     2281 ggccggggcg ttcctgttcc tgctctatat ctgggtgacg ctcggctccg gcttccgctt
     2341 cggcgcgcgc tatctcatct ccgagctggt gatgagcgtg atcggcttcg gcgtcgtgct
     2401 ctatgtcaac gacttctggc actctcattt cgagcttggc gtcggtctgc tcgtcggcat
     2461 gaccgcgctg agcctgtacg tgctctcgct cgtacgccgg atgttcgacg ccctctcgcg
     2521 cgctgaagcg gccaacgtgg cgaagcggcg cttcatctcg atggtgagcc acgagctgcg
     2581 cacgccgctg aacgccatca tcggcatggc cgacctgctg cgcgacaccc agctcaaccg
     2641 cgagcaggcc gacatgctgc agacgctgcg cagctcctcg cgcgtaatgc tcgggctgat
     2701 cgaggacgtg ctcgacttct ccaagatcga ggcgggcaag gtcctgctgg aaaagaccga
     2761 tttcgacctg cacgcgctcg tcaacagcac ctgccgcatc gtctcggcgc aggcggcggt
     2821 caagggcgtg gacttcgtcg tgtcgatcat gccggaagtg ccgcccgcgg tgcgcggcga
     2881 cccgcaccat ctgcgccagg tgctgatcaa cctcgccggc aatgcggtca agttcaccga
     2941 ccgcggtagc gtgacggtgc acgtgtcggt gcaggccgag accgagacca gcgtgcggct
     3001 caagttctcc ctccgcgaca ccggcatcgg catcgcgccc gaggcgcagg ccaagatctt
     3061 cgagagcttc acgcaggcag acgagtccac gacgcggcgc ttcggcggca gcggactcgg
     3121 caccacgatt gccaagcagc tggtgcagct gatgggcggg cgcatcggcc tggagagcgc
     3181 cattggcctg ggcagcacgt tctgggtgga gatcgacctc gagaagcagc ccgagcaggc
     3241 cggcgccgcg gccggcgacc tcgccggagc gcgcgtcctc ctcgtcggct ttccggcgcg
     3301 cgagcgcgcc gcgctcgaag cggcgctggc gggctggggc gcggcgccga ccgcggtggc
     3361 gaacgtggag gaaggcgtgg cgcggctcgt cgcggacatc agcctcgcca agccgtacca
     3421 cagcgcgctg ctctacgcct cgggcgagaa ccgcgagctc gcgcagcgct tccggcgcac
     3481 cgcgcccaac ccggcgccgc ccacgatcct tgcggtgccg cgcgaggccg atgtgcgacg
     3541 cttcgaggtg ctgtccgcgg ggttcgcctc ggtgctggag ctccccttcg acaagcgccg
     3601 gctcttcaac gtgctgcact cggtcgccgc cggcgaggag gtgcacgagg gggtggtgcg
     3661 gcttcaggac tatgcgcggc gcagcgccgc gatcaaggta cgcgtgctcg tggccgacga
     3721 caacgcgacc aaccgcgagg tgatcggcaa gatcctcgag cgcagcggcc acgccattac
     3781 gctggtgaac gacggcgagc agacgctgga cgccctggag cgcgagcccc acgacatcgt
     3841 gatcctcgac cgcaacatgc ccgggatggg cggcatggag gcgctgcagg cgattcgcct
     3901 catgacgcgt tcgcgcgagc ggctgccggt catcatgctc tcggccgacg tcacgccgga
     3961 ggtgaagcgc gaggcgctcg aggcgggtgc ggacagcttc ctgcccaagc ccgtcgcggc
     4021 attgcggctg ctcgacgagg tcgagcgcct cgccggcaag ccggaagttc agcgcaagga
     4081 ggcgcccgcg gcggcggtgc cgcgcgaagc ccccagcgcc cccccgatcg tcaacgccga
     4141 gacgctcggc cacctggagg agctcggctc ctcgcttgca ttcgtcgagc gcctgatcaa
     4201 cgtcttcgtg tccgattcga gcggcatcct tgagcggatc gaaaaggtgc tg
//
LOCUS       DASUNT010000441         3193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_326437, whole genome shotgun sequence.
ACCESSION   DASUNT010000441 DASUNT010000000
VERSION     DASUNT010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326437"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="VFX94_08910"
     CDS             complement(<1..1401)
                     /locus_tag="VFX94_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768339.1"
                     /translation="MTPPFWMLWPDNPALSILVVALIAMVFLYAARKPVHELLHALGA
                     LVGGPLRIAARWLVSTATEMKQRNKAVLLAHGRQEASQRIEREFERLGAVVRRELQGY
                     PTLQRKLLDEITRIEEDYRKCGEVPPPPPDWVEAVTAMANVKTGGGEMVQRILEEIKS
                     SITKIHDKAIGEYRRSYESRHKILEGFMPFWRSVDRTLTEVNKKLADLNESASAVDSL
                     MTKYEAINAKTDKAQHALTVSAFTQFAIAFLVMAVAAGGAFINYKLIALPMSEMVGAG
                     DYLTSSLRTSEVAALVIIFVEASMGLFLMEALRITHLFPRIATLNDRMRRRMMWISLL
                     LLVTLAGVEAALALMRDMLIADKMALIQSLATVQQAQVTDGWVSRIPTAGQMLLGFIL
                     PFALAFVAIPLESLIHSSRTVGGVLLAALVSVLALALRVLGNASRKMSRVLITLYDVA
                     IVIPLTVEHMAKSGISL"
     gene            complement(1525..2226)
                     /locus_tag="VFX94_08915"
     CDS             complement(1525..2226)
                     /locus_tag="VFX94_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5768340.1"
                     /translation="MQRFGVREVEKLLRLPRRTIHGLVRAGFVSPARGPRQAYLFSFQ
                     DLIVLRTAQALAVAKVPARRIAASLRELRRHLPEAMPLSGLAIGAEGDRVVVREGVSR
                     WQADSGQYLLAFEGDPGAGALRVIEKHVSLEDEIDQGYLLHESGRLKEAEAAYRAALD
                     QFGPDPLLLYNLGVLLEDLGRKTEAAAAYRAALQRDPAMADCHYNLALLCEQLRRPQE
                     AIRHMAQYRKLVAQK"
     gene            complement(2228..3067)
                     /locus_tag="VFX94_08920"
     CDS             complement(2228..3067)
                     /locus_tag="VFX94_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX5768341.1"
                     /translation="MAVRSIASLTISFGMVSIPVKLYSATEASRAMSFNLLHKTCGSR
                     LRQQYFCVKEDVPVGRGDMAKGYEFAKDQYVMFTSEELKALEEAGTHTADITEFVPAK
                     TIDPVYFDKAYYLGPDKGGAKPYALLARALKETGRCALGRWAARGKQYIVMIRPMDGG
                     LVEGLVMQQLLYAGEVRSINDIDIPKTDVRDAELKLAQQLIEQQTSEKFDPSQYTDAV
                     RARVEAAVQRKVEGKEITMAEAPQEGAQVIDLMEALRASLEKKPAKKPQPVKPVSKRA
                     AKK"
BASE COUNT          530 a         1070 c          997 g          596 t
ORIGIN      
        1 cagcgaaatg ccggacttcg ccatgtgctc gaccgtcagc gggatcacga tcgccacgtc
       61 gtagagcgta atcagcaccc tggacatctt gcgcgaggcg ttgcccagca cccgaagagc
      121 cagcgcgagc accgagacca gcgccgcgag caggacgccc ccgacggtgc gtgacgaatg
      181 gatgagcgac tcgagcggaa tggcgacgaa agcgagcgcg aacggcagga tgaagccgag
      241 cagcatctgt cccgcggtgg gaatgcggct cacccagccg tcggtgacct gcgcttgctg
      301 cacagtggcg agcgactgga tcagcgccat cttgtccgca atcagcatgt cgcgcatcag
      361 ggcgagcgcc gcctccacgc ccgccagcgt gacgagcagc agcagcgaga tccacatcat
      421 ccggcggcgc atccggtcgt tgagggtcgc gatgcgcggg aaaaggtgcg tgatgcgtag
      481 cgcctccatc aggaacaggc ccatcgacgc ctcgacgaag atgatcacca gcgcggccac
      541 ctccgaggtg cgcaggctgc tcgtcagata gtcccccgcg ccgaccattt ctgacatcgg
      601 cagcgcgatc agcttgtagt tgatgaaagc gccgccggcg gccaccgcca tcacgagaaa
      661 cgcgatggca aattgagtaa aggccgatac cgtcagcgca tgctgcgcct tgtcggtctt
      721 cgcgttgatc gcttcgtact tggtcatcag cgaatcgacc gcgctagcgc tttcgttcag
      781 gtcggcgagc ttcttgttga cctcggtcaa cgtgcggtcc acggagcgcc agaacggcat
      841 gaacccctcg aggatcttgt ggcgcgactc gtaggaccgg cgatactcgc cgatcgcctt
      901 gtcgtgaatc ttggtgatcg agcttttgat ctcctccagg atcctctgca ccatctcgcc
      961 gccgccggtc ttgacgttcg ccatggcggt gaccgcctcg acccagtccg gaggcggcgg
     1021 tggaacctcg ccgcacttgc ggtagtcctc ttcgatcctg gtgatctcgt cgagcagctt
     1081 tctctgcagc gtggggtagc cctgcagctc gcggcgcacg accgctccga gccgctcgaa
     1141 ttcgcgctcg atgcgctggc tcgcctcctg gcgtccgtgc gcgagcagta ccgccttgtt
     1201 ccgttgcttc atctccgtcg cggtgctcac gagccagcgc gccgcgatac gcagcgggcc
     1261 gccgaccagc gctccgagcg cgtggagcag ctcgtgcacc ggcttgcgcg cggcgtagag
     1321 aaagaccatc gcgatcaggg ctaccacgag gatcgagagc gcagggttgt ccggccacag
     1381 catccagaat ggaggcgtca tcgcgttctc cttcccttcg gaatttcaga gagggtagaa
     1441 caggatcggc gcgcgggggc gactacctcg cggcctttgc gcattaagtc tcgaatgctg
     1501 cgcccgcgcc gtgaaatgct ggcgttactt ttgcgcgacg agcttgcggt attgcgccat
     1561 gtggcgaatc gcctcctgcg gccggcgcag ctgctcgcag agaagcgcga ggttgtagtg
     1621 acaatcggcc atcgccggat cgcgctgcag cgcggcgcgg tacgctgcgg cggcttcggt
     1681 cttccggccg agatcctcga gcaggacgcc gaggttgtag aggagcagcg gatccgggcc
     1741 gaactgatcg agcgccgcac ggtaggccgc ctccgcttcc ttcagcctgc ccgactcgtg
     1801 cagcaggtag ccttgatcga tctcgtcctc gagcgagacg tgcttttcga tcactctgag
     1861 cgcgccggct cccgggtctc cttcgaaggc gagcagatac tgccccgagt cggcctgcca
     1921 gcgactcacg ccctccctca ccaccacgcg atcgccctcc gcgccaatgg cgagccccga
     1981 gagcggcatc gcctccggca ggtggcggcg caactcacgc agcgaggcgg cgatgcgccg
     2041 cgccgggact ttcgctaccg cgagcgcctg cgcggtgcgc agcacgataa ggtcctggaa
     2101 cgagaaaagg tacgcctggc gcgggccgcg ggcgggagat acgaatcccg cccggaccaa
     2161 tccgtgaatt gtcctgcgcg gcaggcgcag gagcttctcc acctcccgca ccccgaagcg
     2221 ttgcacctta cttcttcgcg gctctcttcg aaaccggttt cacgggctgg ggcttcttcg
     2281 cgggcttctt ttccaggctg gcgcgcagcg cctccatgag atcgatgacc tgtgcgcctt
     2341 cctgaggcgc ttcggccatg gtgatctcct tgccctccac ttttctctgc accgcggcct
     2401 ctacgcgcgc ccgcaccgcg tcggtgtatt ggctcgggtc gaacttctcg gaggtctgct
     2461 gctcgatgag ctgctgggcg agcttgagct ccgcgtccct cacatccgtc ttcgggatgt
     2521 cgatgtcatt gatcgagcgc acctcgccgg cgtacagcag ctgttgcatg acgaggccct
     2581 cgaccaggcc gccgtccatc ggccggatca tcacgatgta ctgcttgccc cgcgcagccc
     2641 agcggcccag cgcgcagcgc ccggtctcct tgagcgcccg ggcgagcagc gcgtacggtt
     2701 tcgcgccgcc cttgtcaggc ccgaggtaat aggccttgtc gaagtacacc ggatcgatcg
     2761 tcttcgccgg cacgaattcg gtgatgtcgg cggtatgggt tccggcctct tccagcgcct
     2821 tgagctcctc ggacgtgaac atcacgtact ggtccttcgc gaactcgtag cccttcgcca
     2881 tgtccccgcg gccgaccgga acgtcctcct ttacgcagaa gtactgctgc cgcaggcgcg
     2941 agccgcatgt tttgtggagc aggttgaacg acatggcacg actcgcctcg gtagccgagt
     3001 agagcttcac cgggatcgac accatgccga acgaaatcgt gagcgacgcg atcgatctga
     3061 ctgccatatt tgacctctac ctcaataact taacggtctg cttcacgcga aggctgcgaa
     3121 ggatagcgca ttatgccttc gcacgttcca gggcggcttc caggctctgc tttttcttca
     3181 gcgcgtcgcg cca
//
LOCUS       DASUNT010000442         3762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_413526, whole genome shotgun sequence.
ACCESSION   DASUNT010000442 DASUNT010000000
VERSION     DASUNT010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413526"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFX94_08925"
     CDS             complement(<1..215)
                     /locus_tag="VFX94_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768342.1"
                     /translation="MRKLLLAVALLVPVAAPAALDIKDVGLGANEQDIKRALPSAHCR
                     PLQWESKAADRRCDDSRVGFGGVEVQVT"
     gene            complement(212..2557)
                     /locus_tag="VFX94_08930"
     CDS             complement(212..2557)
                     /locus_tag="VFX94_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEX5768343.1"
                     /translation="MKVTVIGGGPGGLYFALLAKKAWPRWDITVYERNRPDDTFGFGV
                     VFSDQTLDTFKAYDLVTYERIRRRFAYWGDVDVVYKGQVMRSGGNGFCGCSRVALLNI
                     LQDRGRELGVHMRFQREVDSLEEFPDSDLIVVADGINSKIRARYAEHFQPAVDLRPNK
                     FTWLGSTRPLDAFKYFFRETPQGVLLAHCYQYEADRSTWVIELNEDTWRNFGFDRLGE
                     QEMIPVLEKIFAKELDGHPLIANRSLWRNFPNITNKTWVRGNAVLVGDAKATAHFSIG
                     SGTKLAMEDAIALFEAFRRNQDVATALQVYDTARREEVEKTQHAANVSLAWFEHMKRY
                     WGMEPLQFAFGVMSRSKQITWENLELRDPRFIREIHRWFAQKVKAQGFDVDLQNPPVP
                     MFTPFRLRDMVVENRVVVSPMDQYSAVDGVPGDWHLVHLGSRAIGGAGLVYVEMTCPS
                     PEARISPGDTGLWNETQRDAFKRIVDFCHASSRAKLCMQLGHSGRKGSTQLGWERMDY
                     PLDGGNWPVISASPIAYYEGISQVPREMTRADMEKVLHDFVVSTQYADQAGFDMLELH
                     MAHGYLLASFISPLTNQRNDSFGGSIENRMRFPLEVFRACREAWPKRKPMSVRISATD
                     WAPGGLTGEDLIALTRMLKEAGCDLIDCSTGQTVPHQKPVYGRMYQAPFADWVRNEVG
                     IATMTVGAVTTPDQVNTLLAAGKADLVALARPHLANPYFTLQASAWYQHMGQYWPPQY
                     LPGRDQALRNAARERAEQMDLRIKARPASHEVKEGPVKRAA"
     gene            complement(2554..2943)
                     /locus_tag="VFX94_08935"
     CDS             complement(2554..2943)
                     /locus_tag="VFX94_08935"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5768344.1"
                     /translation="MNILQPPGWARPKGFSNGIACNGTLVFIAGQIGWTGEGKWEARD
                     FAGQFRQALANILSILREAKGGPEHIVRLTWYVVDKKEYLNALKAVGEAYRDLMGRHY
                     PTMAVVQVSGLVEPEARLEIEATAVIP"
     gene            complement(2940..>3762)
                     /locus_tag="VFX94_08940"
     CDS             complement(2940..>3762)
                     /locus_tag="VFX94_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5768345.1"
                     /translation="SSVLLEKPTPESLLETIERFRASVCFAAPVMYRGMAALAGDFDL
                     SSLKKCVSAGEALPDATRQAFKQATGIEIIDGIGSTEMIHIFISHTPERVRRGATGYA
                     IPGYKAAVLDEDGRPCAAGKVGRLAVKGPTGCRYLADERQKSYVRNGWNITGDAYMAD
                     SDGYFFYQARTDDMIISAGYNIAGPEVESALLLHPAVAECGVIGWPDENRGTIVKAFV
                     VLKAGNQKSEDTKKLLQEHVKKVIAPYKYPRAIEFVDALPRTETGKLQRFKLRSA"
BASE COUNT          608 a         1248 c         1214 g          692 t
ORIGIN      
        1 gtcacctgca cttcgacgcc gccgaagccg acgcgcgagt cgtcgcagcg gcggtcggcg
       61 gccttgcttt cccactgcag cggccggcaa tgcgcgctcg ggagcgcgcg cttgatgtcc
      121 tgctcgttcg cgccgagccc gacgtccttg atgtccagcg ccgcgggcgc ggccaccggg
      181 acgaggagcg ccacggcaag aagaagtttc ctcatgcggc tcgcttgacc gggccttcct
      241 tcacctcgtg gctcgccggc cgcgccttga tgcgcaggtc catctgctcg gcgcgctcgc
      301 gcgcggcgtt gcgcaaggcc tggtcccggc cggggaggta ctgcggcggc cagtactggc
      361 ccatgtgctg gtaccaggcc gatgcctgca gcgtgaagta aggattggcg agatgcgggc
      421 gggcgagcgc gacaagatcg gccttgcccg cggcgaggag tgtgttcacc tggtcgggcg
      481 tggtcaccgc gccaaccgtc atcgtggcga tgccgacttc atttctcacc cagtcggcga
      541 agggcgcctg gtacatccgc ccgtacaccg gcttctggtg cgggaccgtc tggccggtcg
      601 agcaatcgat caggtcgcag cccgcctcct tcagcatgcg ggtgagcgcg atcaggtcct
      661 cgcccgtgag cccgcccggc gcccagtcgg tggcggaaat gcgtacggac atcggctttc
      721 tcttcggcca ggcctcgcga caggcgcgaa acacttccag ggggaagcgc atgcggttct
      781 ctatgcttcc gccgaaagaa tcgttcctct ggttggtcag gggggagatg aagcttgcga
      841 gcaggtagcc gtgcgccatg tggagctcga gcatgtcgaa gcccgcctgg tccgcatact
      901 gcgtcgaaac gacgaaatcg tgcagcacct tttccatgtc cgcgcgcgtc atctcccgcg
      961 gaacctgcga gatgccttcg tagtaggcaa tcggcgaagc cgatatcacc ggccagttgc
     1021 cgccgtcgag cggatagtcc atgcgctccc agccgagctg cgtcgagccc ttgcggcccg
     1081 aatggccgag ctgcatgcac agcttcgccc tggagcttgc atgacagaaa tcgacgatgc
     1141 gtttgaaggc gtcgcgctgg gtctcgttcc acaggcccgt gtctcccggc gagatgcgcg
     1201 cttccggaga ggggcaggtc atctccacat agaccaggcc cgcgccgccg atcgcgcggc
     1261 tgcccagatg cacgagatgc cagtcgcccg gcacgccgtc caccgcggag tactggtcca
     1321 tcggcgagac gacgacccgg ttctccacca ccatgtccct caggcgaaag ggcgtgaaca
     1381 tcggcaccgg cgggttctgc aggtcgacgt cgaagccttg cgctttcacc ttctgcgcga
     1441 accagcgatg gatttccctg atgaagcgcg gatcgcgcag ctccaggttt tcccaggtga
     1501 tctgcttgga gcgcgacatc acgccgaagg cgaattgcag cggctccatg ccccagtagc
     1561 gcttcatgtg ctcgaaccag gcgagcgaca cattggccgc atgctgcgtc ttttccacct
     1621 cctcgcgccg cgcggtgtca tacacctgca acgcggtagc gacgtcctgg ttacgccgga
     1681 atgcctcgaa caaggcgatg gcgtcttcca tggcgagctt ggtgcccgag ccgatggaga
     1741 aatgcgccgt cgccttggcg tcgccgacca gcacggcgtt gccccgcacc caggtcttgt
     1801 tggtgatgtt ggggaaattg cgccacagcg agcggttcgc gatcagcgga tggccgtcga
     1861 gctccttggc aaagatcttt tccaggaccg ggatcatttc ctgctcgccc agcctgtcga
     1921 agccgaaatt gcgccaggtg tcctcgttca gctcgatcac ccaggtggag cgatcggcct
     1981 cgtactgata gcagtgcgcg agcagcacgc cctgcggcgt ctcgcggaag aagtacttga
     2041 acgcgtcgag cggcctcgtc gatccgagcc aggtgaactt gttgggccgc aggtccaccg
     2101 caggctggaa atgctccgcg tagcgggccc ggatcttcga attgatgccg tccgcgacga
     2161 cgatgaggtc cgaatcggga aattcttcca gcgagtccac ctcgcgctgg aagcgcatgt
     2221 gcacaccgag ctcgcggccg cggtcctgca ggatgttcag cagcgccacg cgcgagcagc
     2281 cgcaaaaccc gttcccgccc gagcgcatca cctgcccctt gtagaccacg tccacgtcgc
     2341 cccagtaggc gaagcgccgc cggatgcgct cgtaggtcac gaggtcgtag gccttgaaag
     2401 tgtcgagcgt ctggtcggag aacaccacgc cgaagccgaa ggtgtcgtcg ggacggttgc
     2461 gctcgtacac cgtgatgtcc cagcgcggcc acgccttctt cgcgaggagg gcgaaataaa
     2521 ggccgccggg cccgcctccg ataacggtga ctttcatggg attaccgctg tcgcttcgat
     2581 ctcgaggcgc gcttcgggct cgaccaggcc gctcacctgc accaccgcca ttgtcgggta
     2641 gtgccggccc atgaggtcgc gatacgcttc tccgaccgcc ttcaacgcgt tcaggtattc
     2701 cttcttgtcg accacgtacc aggtgagccg cacgatgtgc tcggggccgc ccttcgcctc
     2761 cctgaggatc gagaggatgt tcgcgagcgc ctgtcggaac tgcccggcga aatcgcgcgc
     2821 ctcccatttg ccctcgccgg tccagccgat ctggccggcg atgaagacga gcgtgccgtt
     2881 gcacgcgatg ccgttggaga agcccttggg acgcgcccag ccgggcggtt gcaggatatt
     2941 catgcggacc tcagcttgaa gcgctgcagc ttgccggttt cggtgcgcgg cagcgcgtcg
     3001 acgaactcga tcgcgcgcgg gtacttgtac ggcgcgatca ctttcttcac gtgctcctgc
     3061 aggagcttct tggtgtcctc gctcttctgg tttccggcct tgagaacgac gaacgccttc
     3121 acgatggtcc cccggttctc atccggccat ccgatcaccc cgcactccgc gaccgcggga
     3181 tgcagcagca gcgcgctctc gacttccggg ccggcgatgt tgtagcccgc cgagatgatc
     3241 atgtcgtcgg tgcgcgcctg gtagaagaag tagccgtcgg agtcggccat gtacgcgtcg
     3301 ccggtgatgt tccagccgtt cctgacgtag ctcttctgcc gctcgtcggc gaggtagcgg
     3361 catccggtcg gtcccttgac cgcgagccgc ccgaccttgc ccgcagcaca cggtctgccg
     3421 tcctcatcca ataccgccgc cttgtagccg ggaatcgcat atccggtggc gccgcgccgc
     3481 acgcgctccg gggtgtgcga gatgaagata tggatcatct cggtcgagcc gatgccgtcg
     3541 atgatctcga tgccggtcgc ctgcttgaac gcctggcgcg tcgcgtcggg cagggcttcg
     3601 cccgcggaga cgcatttttt cagggaagaa agatcgaaat ctccggcgag cgccgccatg
     3661 ccgcgataca tcaccggcgc ggcgaagcaa accgaggccc tgaagcgctc gatggtctcc
     3721 agcagcgatt cgggcgtggg cttctcgagg agcacgctgg ag
//
LOCUS       DASUNT010000443         3107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_676030, whole genome shotgun sequence.
ACCESSION   DASUNT010000443 DASUNT010000000
VERSION     DASUNT010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676030"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1670
                     /locus_tag="VFX94_08945"
     CDS             <1..1670
                     /locus_tag="VFX94_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768346.1"
                     /translation="QPDAKEFVALAVCGALTFVWCRHIAGAPQGLAGEGVLAAWGDYF
                     VHGGAISSFGDPRAVGRQAIEFADFPARAYHHASYLLPAALAAPLDLPGLPLATSVWL
                     PLGFLTMCAGAYALGAVLAGAAGGLAALAAIALIPDASIYGLRNGVFSFHWNVVVHPG
                     AAYGTGLTLFAFALLRRWTANADARTLLVSAALSAGTGMFRVHAFLVGFPAWLASAAL
                     ATRFVQQRKLTCFSAAALAFAVFAGAYYAGSGSAPALPGFLRISHTEQEPTAYTGWYQ
                     ALLDAHGPAVTLPAGLLLVLAASLGGLLLVYPLVAALVRASGTAPALEAAPAAAAAAY
                     ALLMIAAPMPPFGYPHDLAYNSFALPYAVLAVWTAATLVAWVAARDERRMPPRLWRAL
                     VLSAVAALPLLWTGARTLAAPKFAWGLQFDSLRLAPGMPQAAAFLREHSRPGQIFAVQ
                     GLSLRWADVDAGTQLGALTGMPAYLARPAIQMSGSERASAAMARHGALRQVAQETSPA
                     AALRRLRELGIAWYVVADQTGPRWDPERRAAAFTSGSVAVYSAADSR"
     gene            complement(1767..2321)
                     /gene="slmA"
                     /locus_tag="VFX94_08950"
     CDS             complement(1767..2321)
                     /gene="slmA"
                     /locus_tag="VFX94_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HEX5768347.1"
                     /translation="MPRAKGERRLEILKALAQMLEAPKWEKITTAALAEKLGVSEAAL
                     YRHFASKAQMYEGLIEFIESSVFTLANLITADESDGHRQCEKLVEMLLAFAEKNPGMV
                     RVMTGDALVGEHERLQARMNQFFERLESTLKQSLRVASDDDAAGRAGALLRYVLGCLH
                     QYAKSGFAKKPTESFSSQRRYLST"
     gene            complement(2340..2999)
                     /locus_tag="VFX94_08955"
     CDS             complement(2340..2999)
                     /locus_tag="VFX94_08955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEX5768348.1"
                     /translation="MKIARLAPSRRVWIFDLDNTLHDASVHIFPSMNMQIHDFLKRQF
                     GVDDEGANRMRRDFWLRYGTTLRGLMRHHGTDPRHFLAETHVFPELADMVVHENAVRH
                     ALARLGGTRLVFSNAPRHYVEEVLRALRLRRFFDAVYAIEDTGYRGKPHAWGFHRLLR
                     NHGLDPHRCALVDDILDNLRAAHCLGMSTVWVAPGGRRVPRYVDVRVSSVTELPRRIG
                     E"
     gene            complement(3003..>3107)
                     /locus_tag="VFX94_08960"
     CDS             complement(3003..>3107)
                     /locus_tag="VFX94_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX5768349.1"
                     /translation="TGVRSVHIIDGRVPHALLLEVLTDEGVGTLIKSK"
BASE COUNT          416 a         1094 c         1067 g          530 t
ORIGIN      
        1 tgcagcccga cgctaaggag ttcgtcgccc tcgccgtgtg cggcgcgctg acattcgtct
       61 ggtgccgcca catcgccggc gccccgcagg gcctcgcggg cgagggcgtg cttgcggcct
      121 ggggagacta cttcgtccac ggcggcgcga tttcgtcgtt cggcgacccg cgtgccgtcg
      181 gccgtcaggc gatcgagttt gccgattttc cggcgcgcgc gtaccaccac gcgtcgtacc
      241 tcctccctgc tgcgctcgcc gcgccgctcg acctgcccgg cctgccgctc gccacctcgg
      301 tctggctgcc gctcggcttc ctgacgatgt gcgcgggcgc ctatgcgctc ggcgcggttc
      361 tcgccggtgc cgcgggcggt ctcgccgcgc tcgccgccat cgcgctcatc cccgacgcgt
      421 cgatatacgg gctgcgcaac ggagtgttca gctttcactg gaacgtcgtg gtccaccccg
      481 gtgcggccta cggcaccggc ttgacgctct tcgccttcgc gctgttacgg cgctggaccg
      541 ctaacgcaga tgcgcgcacg ctgcttgtga gcgcggcgct ctccgccggc acgggtatgt
      601 tccgcgtgca tgcctttctg gtcggatttc cggcctggct ggcgagcgcg gccctggcga
      661 cgcggttcgt gcagcaacgc aagctcacct gcttcagcgc ggccgcgctc gccttcgctg
      721 tgttcgccgg tgcgtactac gccggctccg ggtccgcgcc ggcgctcccg ggcttcctga
      781 gaatctcgca taccgagcaa gagccgaccg cctacaccgg gtggtatcaa gccctgctcg
      841 atgcgcacgg gccggccgta acgctgcccg cgggcctgct gctcgtactc gccgcgagcc
      901 tgggcggctt gctcctcgtc tatccgctag tggcggccct cgtgcgcgca tccgggacgg
      961 cgccggcgct cgaggccgcg ccggcagcag cggcagcggc gtacgcgctc ctcatgatcg
     1021 cggcgccgat gccgcccttc ggctatccgc acgacctcgc gtacaactcc ttcgcgctgc
     1081 cctacgccgt gctcgcggtg tggaccgcgg ccacgctcgt cgcctgggtc gcggcgcgcg
     1141 acgagcgccg gatgccgccg cgcctgtggc gggcgctggt gctgagcgca gtcgccgcgc
     1201 ttccgctcct gtggacaggc gcgcgcaccc tcgcagcgcc gaagttcgca tggggacttc
     1261 agttcgattc gctgcgcctc gcaccgggca tgccgcaggc ggcggcgttt ctgcgcgagc
     1321 attcacggcc cggacagatc ttcgcggtgc agggactgag cctcaggtgg gcggacgtcg
     1381 acgcgggcac gcagctcggc gcactcacgg gcatgccggc ttacctcgcc cgccccgcga
     1441 tccagatgtc cggcagcgag cgggcaagcg ccgcgatggc gcggcatggg gcgcttcggc
     1501 aggtcgcgca ggagacgagc cccgccgcag cgctacgccg cctgcgcgag ctcggcatcg
     1561 cgtggtacgt cgtggccgac cagaccgggc cgcgctggga ccccgagcgc cgcgcggccg
     1621 ctttcacttc gggcagcgtc gccgtctatt cagccgctga tagccggtag ccaggcgcag
     1681 aaacggcggg gaacccgcgc ggcgcgagcg ggggcaatcg gctcgctgcg ctgtccaaga
     1741 aggaacgtgg tccgccgttt atctcgtcag gtcgacagat agcgtctttg cgacgagaag
     1801 ctctcggtcg gcttcttagc gaagccgctc ttggcgtact ggtgcaggca gccgagcacg
     1861 tagcgcaaca gagcacccgc gcggccggcg gcgtcgtcgt cggacgcaac gcgcagcgac
     1921 tgcttgagcg tcgactccaa gcgctcgaag aactggttca tgcgcgcctg cagccgctcg
     1981 tgctccccga cgagcgcgtc gccggtcatg acgcgcacca ttccggggtt cttctcggcg
     2041 aacgcgagca gcatctcgac cagcttttcg cactggcggt ggccgtcgga ctcgtcggcg
     2101 gtgatcaggt tggcgagcgt gaagaccgag ctctcgatga actcgatcaa accctcgtac
     2161 atctgggcct tggaagcgaa atggcggtag agcgctgctt ccgagacgcc gagcttttcg
     2221 gcaagcgcgg cggtagtgat cttctcccac ttcggcgcct ccagcatctg ggcgagtgct
     2281 ttcaggatct cgagccggcg ttcgcctttg gcgcggggca tgtcagccga atccagtcct
     2341 cactctccga ttctacgcgg cagttcggtc acggaggaaa cgcgtacgtc gacgtaacgg
     2401 gggacgcgcc gcccgcccgg ggcgacccag accgttgaca tgccgaggca atgcgccgcg
     2461 cgcaggttgt cgaggatatc gtcgacgagc gcgcaccgat gcgggtcgag gccatggttg
     2521 cgcagcagcc ggtgaaagcc ccacgcgtgc ggcttgccgc ggtatccggt gtcctcgatg
     2581 gcgtacaccg cgtcgaagaa ccggcgcagc cgcagggcgc gcagcacctc ctccacgtag
     2641 tgccgcggcg cattggagaa gacgagccgc gtgccgccga gccgagcgag ggcgtggcgc
     2701 accgcgtttt cgtgcaccac catgtcggcg agctccggga agacgtgcgt ctcggccagg
     2761 aagtggcgcg ggtcggtgcc gtggtgccgc atgagcccgc gcagagtggt gccgtagcgc
     2821 agccagaagt cccgccgcat ccggttggcg ccttcgtcgt ccacgccgaa ctggcgcttg
     2881 agaaagtcgt ggatctgcat gttcatcgac gggaagatgt gcacgctcgc gtcgtgcagc
     2941 gtgttgtcga ggtcgaagat ccacacccgc cgcgatggcg ccagcctcgc gatcttcacg
     3001 acttacttgc tcttgatcag cgtgccgacc ccttcgtcgg taagcacttc caggagcagc
     3061 gcgtgcggca cgcgcccgtc gatgatgtga acgcttctca cgccggt
//
LOCUS       DASUNT010000444         2244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_764011, whole genome shotgun sequence.
ACCESSION   DASUNT010000444 DASUNT010000000
VERSION     DASUNT010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764011"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..928)
                     /locus_tag="VFX94_08965"
     CDS             complement(131..928)
                     /locus_tag="VFX94_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX5768350.1"
                     /translation="MRRVVFNHKGGVGKSTIACNLAAVSAKGGARVLVVDLDPQGNST
                     QYLLGKHGRELEPTLAGFFEQVLSFPLRPKKPDEFVHGTLFERLDVLPSHPLLAQQQD
                     KLEARYKIFKLRDALVELDKQYDFIYVDTPPALNFLTRSALIAADCCLIPFDCDDFSR
                     RALYTLLDDIAEIRADHNSGLVVEGIVVNQFLPRARLPQKMVQELIGEGLPVLQPYLS
                     SSVKVRESHECAKPLIYLDPHHKLTHEFIALHCGLEERRRGEQRLRA"
     gene            1059..1427
                     /locus_tag="VFX94_08970"
     CDS             1059..1427
                     /locus_tag="VFX94_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEX5768351.1"
                     /translation="MKLHASRPSGVNTITGYGEGYVMVNGERRASSVVVLADRIEAWS
                     VERFDALTAEHFRFLTDLEVDIVLLGTGPRQRFPHPRLTAALAQAGIGLEVMDLHAAC
                     RTYNILVAEERKVAAALLFE"
     gene            1424..>2244
                     /locus_tag="VFX94_08975"
     CDS             1424..>2244
                     /locus_tag="VFX94_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5768352.1"
                     /translation="MSLFPGFVRRRIRTSGATINLVAAGEGPPVLLLHGYPQTHVIWH
                     KIAPALARDYTVVCADLRGYGDSSKPRGIPGHANYSKRAMALDMVEAMEALGFVKFHL
                     VGHDRGGRVAHRMARDHGRRVQTLTVLDISPTLKMYQSTNVQFAKAYYHWFFLIQEAP
                     LPEQMLSNIGPAYILLRLGRGKSGLKVFDRRALAEYARCFDPHSTCEDYRAAATIDLV
                     HDRKDLHRKLKMPVLALWGRQGVIQALFDCLADWREVASDVRGRALQCGHFIPEE"
BASE COUNT          405 a          736 c          711 g          392 t
ORIGIN      
        1 atcttcagct cgttggtgat gttgaaaacg tacggcggca gccgcttgat cctggaaaac
       61 tcacgcattg cacgattata ggaccctctt gcgcaaggag gccttcacgg caatcttgcc
      121 gtcgcaaaag ctaggcgcgc aagcgctgct cgccgcgccg cctttcctcc agtccgcagt
      181 gcagcgcaat gaattcgtgc gtgagcttgt ggtgggggtc aagatagatg agcggtttcg
      241 cgcactcgtg tgattcccgc accttgaccg aggacgagag ataaggctga agcaccggca
      301 gaccctcgcc gatgagctcc tgcaccatct tctgcggcag acgtgcgcgc ggcaggaact
      361 ggttgaccac gataccttcg accaccagcc cggagttgtg atcggcacgg atttccgcaa
      421 tgtcgtcgag cagcgtgtag agcgcccggc gcgagaaatc gtcgcaatcg aagggaatga
      481 ggcagcaatc ggctgctatg agggcagacc gagtcaagaa attaagagcg ggcggcgtgt
      541 cgacgtagat gaaatcgtac tgcttgtcca gttcgaccag cgcatcgcgc aatttgaaga
      601 tcttgtagcg cgcctcgagc ttgtcctgct gctgcgcgag cagcggatgc gagggcagga
      661 cgtcgagccg ctcgaacagc gtgccgtgca cgaactcgtc gggcttcttc ggtcgcagcg
      721 ggaagctgag cacctgctcg aagaaaccgg cgagcgtcgg ctcgagttcc cggccatgct
      781 tgccgaggag gtactgagtc gagttcccct gcggatcgag gtcgaccacc agtacgcggg
      841 cgccgccctt tgcgctcact gcagccaggt tacaggcgat cgtcgatttg ccgacaccgc
      901 ctttgtgatt gaagacgact cgacgcatgg ttcctccctt agacgcctcg cagcatgacg
      961 tcggcatcat agtcgtccaa ccgggagttg ccaatcaccg cctggctact agatgtagta
     1021 tccccgccgg tccgaaggtc ggcgagtaga atcctggcgt gaagctccac gcctcgcgcc
     1081 cgagcggcgt caacaccatc accgggtatg gcgaaggcta cgtcatggtg aacggcgagc
     1141 ggcgcgcttc cagcgtcgtg gtgctcgccg atcgcatcga agcgtggtcc gtcgagcgct
     1201 tcgacgcgct caccgccgag catttccgct tcctgacgga cctcgaggtc gacatcgtgc
     1261 tgctcggcac cggcccgcgc cagcgcttcc cgcatccgcg gctcaccgcc gcgcttgccc
     1321 aggcgggcat tgggctcgag gtcatggacc tgcacgctgc ctgccggacc tacaacatcc
     1381 tcgtggccga agaacgcaaa gtcgccgcgg cgctgctctt cgaatgagcc tgttccccgg
     1441 gttcgtccga cgacgaatca gaacgagcgg cgcgaccatc aacctcgtcg ccgccggcga
     1501 gggcccgccc gtgctgctgc tccacggcta cccgcagacg cacgtcatct ggcacaagat
     1561 cgcgcccgcg ctcgcgcgcg actacaccgt ggtttgcgcc gacctgcgcg gctacggcga
     1621 ctcgtccaag ccgcgcggca ttccgggcca cgcgaattac tcgaagcgcg ccatggcgct
     1681 ggacatggtc gaggcgatgg aagcgctcgg cttcgtcaag ttccacctcg tcggccacga
     1741 ccggggcggg cgcgtcgccc accgcatggc gcgggaccac ggccggcgcg tgcagacgct
     1801 cacggtgctc gacatctcgc cgaccctcaa gatgtaccag agcaccaacg tgcagttcgc
     1861 caaggcgtac taccactggt tcttcctgat ccaggaggcg ccgctgcccg agcagatgct
     1921 gagcaacatc ggcccagcct acattctgct gcgcctcggc cggggaaaga gcggcctgaa
     1981 ggtcttcgac cggcgcgcgc tcgccgagta cgcgcgctgc ttcgacccgc attccacctg
     2041 cgaggactac cgcgcggcgg cgaccatcga cctcgtgcac gacaggaaag atctgcatcg
     2101 gaagctcaag atgccggtgc tcgcgctgtg ggggcggcag ggcgtcatcc aggcgctctt
     2161 cgattgcctt gccgattggc gcgaagtggc gagcgatgtg cgcggccggg cgctccagtg
     2221 cgggcatttc atccccgagg agaa
//
LOCUS       DASUNT010000445         2736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_939174, whole genome shotgun sequence.
ACCESSION   DASUNT010000445 DASUNT010000000
VERSION     DASUNT010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939174"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFX94_08980"
     CDS             <1..438
                     /locus_tag="VFX94_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA synthetase"
                     /protein_id="HEX5768353.1"
                     /translation="DGEIHVRTREAWHASGDLGRIDEEGYLYVDGRRKHVLITSYGRN
                     VSPEWPEAELLAGPAIAQAAVFGEAQPRLCALVVPAPGASAAAVEAQVRAANARLPDY
                     AQIAAWLRADAPFTSQNGYATANGRARRDALWQTYGKRLDGLL"
     gene            425..1069
                     /locus_tag="VFX94_08985"
     CDS             425..1069
                     /locus_tag="VFX94_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX5768354.1"
                     /translation="MAFFDELQSRTAAERADLLSVPAIAECLAGRVSHTRYVAFLREA
                     YHHVKHTVPLLMACGSRLGEERAALRDAIAEYIAEEKGHEAWILDDLRACGDDAEAVR
                     KSRPGRATELMVAYVYDYIARVNPAGFLGMVHVLEGTSSAIATPAAGVIARTLELPAA
                     AFTYLTSHGALDQEHVRFFENTVNGLRPEDQEHVVHVAKRVYRLYGDIFRGLPS"
     gene            1066..1842
                     /locus_tag="VFX94_08990"
     CDS             1066..1842
                     /locus_tag="VFX94_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5768355.1"
                     /translation="MNRYRAVLTGASGGIGREIAVALAPCCDELLLVGRNAERLAETA
                     AAAGPCARTLVADLATPQGRDAAVLPGINLLIHNAGAGEFAWLESQGDAALERIVATN
                     VLAPMQLTRRLLPELRRAPEAWIVNVGSIMGYLGYPGHAAYCASKFALRGFGEALRRE
                     LADSTVRVLYLAPRATRTAMNGAGVRALNAELGVAMDEPAAVARELLALLRRPARERL
                     LGMPEKLFARLNQILPGLVDRALLRQLATIRRHASQGGVK"
     gene            1839..2459
                     /locus_tag="VFX94_08995"
     CDS             1839..2459
                     /locus_tag="VFX94_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768356.1"
                     /translation="MRWLVLLTGLVFSLSALAGLEEDVGALQTEWARIKYERPASEQE
                     KAFAELTQNADAVRAKYAGRAEPQIWYGIIAASYAGARGGLGALSIVKDAKKALEQAI
                     EVDPKALDGSAYTSLGSLYYQVPGWPIGFGSDEKAREMLDKALTLNPDGIDPNYFMGD
                     FLYRNGDHAGAKQALDKALKAPARPGRELADQGRRKEIESLLAAMR"
     gene            2480..>2736
                     /locus_tag="VFX94_09000"
     CDS             2480..>2736
                     /locus_tag="VFX94_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5768357.1"
                     /translation="MRILLVEDDELLGAGIEDALTRAHFSLEWVRTGPLAAAALARDG
                     FDAAVLDLGLPGMDGLEVLSRARAAGNRTPVLILSARDAVA"
BASE COUNT          414 a          990 c          953 g          379 t
ORIGIN      
        1 gacggcgaaa ttcatgtgag gacgcgcgaa gcctggcatg cgagcggcga cctcgggcgc
       61 atcgacgagg agggctatct ctacgtcgac ggacgacgca agcatgtcct catcacatcc
      121 tacgggcgca atgtctcgcc cgaatggccg gaagccgagc tcctcgccgg tcccgccatc
      181 gcgcaggcgg cggtgttcgg ggaagcgcag ccgcggctct gcgcgctcgt cgtgccggcg
      241 cccggcgcct cggcggccgc ggtggaggcg caggtgcgcg cggcgaacgc gcgcctgccc
      301 gactacgcgc agatcgccgc ctggctccgc gcggacgcgc ctttcacctc gcaaaacggc
      361 tatgccaccg ccaacggtcg cgcgcggcgc gatgcgctat ggcaaacata cgggaagcgc
      421 ctcgatggcc ttctttgacg agctgcaaag ccgcaccgcg gcggagcgcg cggatctgct
      481 ctcggtcccg gcgatcgccg aatgcctcgc cggccgcgtg agccacacgc gctacgtggc
      541 atttctgcgc gaggcctacc accacgtcaa gcacacggtg ccgcttctca tggcgtgcgg
      601 cagccggctc ggcgaggagc gcgccgcgct gcgcgacgcg atcgccgagt acatcgccga
      661 ggaaaagggc cacgaggcct ggatcctcga cgacctgcgc gcctgcggcg acgatgccga
      721 ggcggtgcgc aaatcgcgac cgggccgcgc caccgagctc atggtcgctt acgtctacga
      781 ctacatcgcg cgcgtgaatc ccgccggctt cctcggcatg gtgcacgtcc tcgagggcac
      841 gagcagcgcc atcgccacgc ccgcggccgg cgtcattgcg cgcaccctcg agctgcctgc
      901 ggccgccttc acctatctca cgtcgcacgg cgcgctcgac caggaacacg tgcgtttctt
      961 cgaaaacacg gtgaacgggc tcaggcccga agaccaagag catgtcgtcc acgttgcgaa
     1021 gcgcgtctat cggctctacg gcgacatttt ccgcgggctg ccgtcatgaa ccgctatcgc
     1081 gccgttctca cgggggcgag cggcggaatc ggccgcgaga tcgccgtcgc gctcgcgcct
     1141 tgctgcgacg agctcctcct cgtcggccgc aacgccgagc gcctggccga gaccgccgcc
     1201 gcggccgggc cgtgcgcgcg cacgctggtc gcggacctcg cgacgccgca ggggcgcgac
     1261 gccgcggtgc tgccgggcat caacctgctc attcacaacg ccggcgcggg agagttcgcc
     1321 tggctcgaaa gccagggcga cgcggcgctc gagcgcatcg tggccaccaa cgtgctcgcg
     1381 ccgatgcagc tcacgcgccg gctgctgccc gagctgcggc gcgcgcccga ggcgtggatc
     1441 gtcaacgtgg gctcgatcat gggctacctc ggctatcccg gccacgccgc ctactgcgcg
     1501 agcaagttcg cgctgcgcgg cttcggcgag gcgctgcgcc gcgagctcgc cgacagcacg
     1561 gtgcgcgtgc tctacctcgc gccgcgcgcg acgcgcaccg cgatgaacgg ggcgggcgtg
     1621 cgcgcgctca acgccgagct cggcgtggcg atggacgagc ccgccgcggt ggcacgcgag
     1681 ctcctcgcgc tcctgcggcg tccggcgcgc gagcggcttc tcggcatgcc cgagaagctc
     1741 ttcgcgcgcc tcaaccagat cctgccggga ctcgtggacc gcgcgctcct gcgccagctg
     1801 gccacgatcc ggcgccatgc atcccaagga ggtgtcaaat gagatggctt gtgcttctta
     1861 cgggacttgt cttctcgctc agcgcgcttg ccggcctcga agaggacgtg ggcgcgttgc
     1921 aaaccgagtg ggcgcgcatc aagtacgagc gtcctgcgag cgagcaggag aaggccttcg
     1981 ccgagctcac ccagaacgcg gacgcggtgc gcgccaagta cgccggccgc gccgagccgc
     2041 agatctggta cggcatcatc gcggcgagct acgcgggcgc gcgcggcggg ctgggggcgc
     2101 tctcgatcgt caaggacgcg aagaaggcgc tcgagcaggc gatcgaggtc gaccccaagg
     2161 cgctcgacgg ctccgcctac acgagcctcg gctcgctcta ctaccaggta ccgggctggc
     2221 cgatcggctt cggcagcgat gaaaaagcgc gcgagatgct cgacaaggcg ctcacgctca
     2281 accccgacgg tatcgacccg aactacttca tgggcgattt cctgtaccgc aacggcgacc
     2341 acgccggcgc aaagcaggcg ctcgacaagg cgctcaaggc gccggcgcgg ccgggacgcg
     2401 agctcgccga tcaggggcgg cgcaaggaga tcgagtcgct cctcgcggcg atgcgctgaa
     2461 aagcgcgtat tctgccgccg tgcgcatcct tctcgtagag gacgacgagc tgctcggcgc
     2521 cggcatcgag gacgcgctca cgcgcgcgca cttctccctg gaatgggtgc gcaccgggcc
     2581 gctcgccgcg gcggcgctcg cgcgcgacgg cttcgacgcc gcggtgctcg acctcggcct
     2641 gccgggcatg gatggcctcg aggtgctctc ccgcgcgcgc gcggcgggca accggactcc
     2701 ggtgctgatc ctgagcgcac gcgacgcggt ggcgca
//
LOCUS       DASUNT010000446         8420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1115135, whole genome shotgun sequence.
ACCESSION   DASUNT010000446 DASUNT010000000
VERSION     DASUNT010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115135"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1955
                     /locus_tag="VFX94_09005"
     CDS             <1..1955
                     /locus_tag="VFX94_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5768358.1"
                     /translation="GYTEALVRALKGEEAIFDTEFRIRDARGDWKWLYARGRVTEHTA
                     GGRAKYVSGTVADIDEQKRAEAALAEREQRFRDVVDASGEYVWETDAQWRYIYLSERV
                     EAVLGYPRAELLGRKPREFMPLGEPKALDQWFAGRAADGQPFRDILHRMITKSGGVIW
                     LSVSGVPVRNAEGDLIGWRGTGADVTARKHAEARIEQLTTRDALTGLPNSTLLADRAS
                     QAILSAARSRSQFALFCIDLDRFTLVNDSLGHRAGDALLRSVAERLGNLVHGEDTLAR
                     LGGDDFVMLQAIRSKGDPAAEAAAAAQRVLGVLARPFTVQGKTLNVAATIGISVYPND
                     GRDFVELLKNADAALHHAKETGKGTWRMFAPALNARAAQRLRLENELRSALTRGELAL
                     YWQPVVRGWRPDRRSSPVHWRGRIVGAEALVRWQHPERGLLGPEHFVPLAEECGLIRA
                     VDEWALERALSQAGAWRRRYGKNLWYAVNVSAPGLAQGAEYLQRLEHALVANGLDGKS
                     VELEVTERVVMSGLAENVATLEAIGKLGVRFAIDDFGTGYSSLAYLRRLPIDKLKIDR
                     SFLGAIDSHGADAAIVRAITALAATLGISVAAEGVETPAQLERLLSLGCEEWQGHYFS
                     PPLDTAGLEELLSESLSARTARSSEA"
     gene            complement(1865..2866)
                     /locus_tag="VFX94_09010"
     CDS             complement(1865..2866)
                     /locus_tag="VFX94_09010"
                     /inference="COORDINATES: protein motif:HMM:NF021466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HEX5768359.1"
                     /translation="MPYLGYGTRERLVMCGRVLEDENFTPATDSDSRWRNLVRFWKRL
                     ESDEVPGARLRARFFGRETQGVTDQEGYFRLEIAPRGRLGPGAWQEVSLELIDRPGAA
                     PAVARIMVPSRRARFGVISDIDDTIVASQVGSKVRMILTAALTNARTRKPFPGVAAFY
                     RALHAGVNPFFYISKSAWNLYAPLLEYLEVQQLPIGPLLLRDFGLRPEKEHKRKAIEE
                     VLAAYPKLGFILSGDSGEQDPEIYSAVVQRHPERIRAIYIRSVNQDPARLLALESLIN
                     EVSKTGCQLVLAGEAEQAAAHAAAEGLIQASELRAVRADSDSDRSSSRPAVSSGGLK"
     gene            complement(2872..3255)
                     /locus_tag="VFX94_09015"
     CDS             complement(2872..3255)
                     /locus_tag="VFX94_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5768360.1"
                     /translation="MAAFGARPAGLGFRKALERHLAAIRDRDLAALAATVADDELVLI
                     GADGKLARTPREFLDAHRAWFDMPGWTLDVAPAVIYETAEMALAVLRLLYRDGESEQP
                     SLLTLVFRKKADRWLMVLDQNTPIK"
     gene            complement(3222..3563)
                     /locus_tag="VFX94_09020"
     CDS             complement(3222..3563)
                     /locus_tag="VFX94_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX5768361.1"
                     /translation="MKLLVVWLINAVALIAVAYLMPSISVASFGSALVAALILGLVNT
                     VVRPLLVLLTLPVTILTLGLFIFVLNGLLFWMVGNWLEGFHVGGFWAGVLGAIVFSLV
                     SWLLSALVLRA"
     gene            complement(3566..4615)
                     /locus_tag="VFX94_09025"
     CDS             complement(3566..4615)
                     /locus_tag="VFX94_09025"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5768362.1"
                     /translation="MGVDKDKARQALDKVFRDMAGAMAAGMVYVGVKTGLLREMAGKG
                     AMTASEVAKRSRLQPRYVEEWLKGMVAAGYLDYDPSAQTYSLPEEQAYFLASDETDHF
                     VGGLFAMVPPLLRVAPDVARAFAAGGGVGFEAFGPECVEALELINGGQYDARFADYWM
                     KSMPQAAERLRAGGRALDVGCGSGRVCIALARAFPQAEIIGIDPDAESIRRARASAGD
                     SRIEFVAGDVFSVDRQSGFDFVTLCDALHDLAEPEKTLERIRSLLKADGTLFIVEPKA
                     ADQLEDNRNSVAATFYGFSLFHCMTQSLARGGPGLGTCLGPAATEALVRRAGYTRFER
                     LDIRSLTNLFYCAQP"
     gene            complement(4606..5364)
                     /locus_tag="VFX94_09030"
     CDS             complement(4606..5364)
                     /locus_tag="VFX94_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEX5768363.1"
                     /translation="MNPELKVYWRPGCSSCVKVKEYLSQQGVPFESIDVSARPEAMRE
                     LLAFGVKTVPVVVRGGEFVFAQALEDVSRFIGREFLQDRLSPVELVAKWLVVLRAAQR
                     HARQLPAARLRERAVESRDRSIRDLAYHIYQIPDAFLQAVEDGVGDLTTIYNAPPPAD
                     VLSTEKIREYGRRIESRLERWWAALPDKSCRQTVNTYYGARPLHELLERCTWHSAQHA
                     RQIIAVLERLGIEPEAPLTEADYAGLPLPKGLWE"
     gene            complement(5361..6098)
                     /locus_tag="VFX94_09035"
     CDS             complement(5361..6098)
                     /locus_tag="VFX94_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX5768364.1"
                     /translation="MTAPSAIPAAARAELAPHGTLRVGLNYGNFLLVLKDAPDGTPRG
                     IAPDLGRALGSRLGVPVKFVKFAQAGQLADAVRDGKCDVGFLGAEPQRAADIAFTKAY
                     LEIPVTFLVPAGSPIRSIADVDREGVRIAVSARSAYDLYLSRTLKHAKLVRAEGIDAS
                     CELFLKETLEALAGLKPRLVSDAEKLPGSRVLEGQVSSVQQAAGTAKARTAGARYLSE
                     FIEDIKRSGLVASTIEKHGVKGVSVVP"
     gene            complement(6215..6565)
                     /locus_tag="VFX94_09040"
     CDS             complement(6215..6565)
                     /locus_tag="VFX94_09040"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5768365.1"
                     /translation="MNKAVKDDRPLYEVERRAEHAARPGFRITELQISKTQKVPWHYH
                     SNVQDTFYVLEGELRIFLQDPKEEVRLRPGDTYTARARRPHLVTNAGESSATFLVLQG
                     IGEYDFVPLVEPRR"
     gene            complement(6562..7365)
                     /locus_tag="VFX94_09045"
     CDS             complement(6562..7365)
                     /locus_tag="VFX94_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5768366.1"
                     /translation="MKREYVHGYDRRANVRLQDQADALVELLHSDSAYPDGSTVLEAG
                     CGVGAQTVTLARRSPGARIVSVDIAAASLTAAKAKVEAAGVANVRFQQADIFALPFEP
                     RSFDHVFVCFVLEHLSRPLAALERLKALVRPGGTVTVIEGDHGSTYFHPDSAAAYDAI
                     GCQAALQRAAGGNANIGRELYPLLVQAGLRNVRVSPRMVYVDSSRPQLVEGFTRKTFT
                     AMIEGVREKAIANGLISPARFDEGIRALYRTAEADGVFCYTFFKGLGEL"
     gene            complement(7443..8108)
                     /locus_tag="VFX94_09050"
     CDS             complement(7443..8108)
                     /locus_tag="VFX94_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX5768367.1"
                     /translation="MFDQYGTIVDMQKGLTEAVTAFLKRKGWDGEPSSFVTWWRRTHF
                     ENSMIDALCDRGHTPYREIGHRAVSYVMDRCHISYTEREVRWLVGEIEKLKPFPDVVA
                     ALEKLRSAGYRLAILSNGDRDMLEAAGPHIGFRFDHVISVQEAGYFKPHWKTYAKAEE
                     IIGEGRSSLLFVANHAFDCIGAKCYGMRTAFIDRRKRPFGQTPHQPDIVIADFSELAA
                     ELA"
     gene            complement(8191..>8420)
                     /locus_tag="VFX94_09055"
     CDS             complement(8191..>8420)
                     /locus_tag="VFX94_09055"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX5768368.1"
                     /translation="VALAMKERRIRIELGSGMESYISNAKAQSIIDTVMVPAFRKGDF
                     AGGLQLALEELMKEARRFVVSPVQVKPSPQQ"
BASE COUNT         1358 a         2833 c         2896 g         1333 t
ORIGIN      
        1 tcggctacac cgaggccctg gtgcgcgcgc tgaagggcga agaagcgatc ttcgacacgg
       61 agtttcgcat ccgtgacgcg aggggcgact ggaaatggct gtacgcgcgc ggccgcgtca
      121 ccgagcacac cgcgggcggc cgcgcgaagt acgtctcggg cacggtcgcc gacatcgacg
      181 agcaaaagcg cgccgaggcg gcgctcgccg agcgcgagca gcgcttccgc gacgtggtcg
      241 acgcctcggg cgagtacgtc tgggaaaccg atgcgcagtg gcgctacatc tacctctctg
      301 agcgcgtcga ggcggtgctc ggctatccgc gcgcggagct tctcggccgc aagccgcgcg
      361 agttcatgcc cctcggcgag ccgaaggcgc tcgatcagtg gttcgccggg cgtgccgccg
      421 acggccagcc cttccgcgat atcctgcacc ggatgatcac caagtcgggt ggcgtgatct
      481 ggctctcggt aagcggggtg ccggtgcgca atgccgaggg cgatctcatc ggctggcgcg
      541 gtaccggcgc cgacgtcacc gcgcgcaagc acgccgaggc gcgcatcgag cagctcacca
      601 cgcgcgacgc gctgaccggg ctgccgaact cgacgctcct cgccgaccgc gcgagccagg
      661 cgattctcag cgcggcgcgc agccgctcgc agttcgccct tttttgcatc gacctcgacc
      721 gattcacgct ggtgaacgac tcgctcggac accgggcggg cgatgccctg ctgcgctcgg
      781 tggccgagcg cctgggcaac ctggtgcacg gcgaagatac gctcgcccgc ctgggcggcg
      841 acgacttcgt gatgctgcag gcgatccgct cgaagggcga tcccgcggcg gaagcggccg
      901 ccgccgcgca gcgcgtcctc ggcgtgctcg cgcggccgtt cacggtgcaa ggcaagaccc
      961 tcaacgtcgc cgcgacgatc ggcatcagcg tctacccgaa cgacgggcgc gacttcgtcg
     1021 agctcctcaa gaacgccgac gcggcgctcc accacgccaa ggagaccgga aagggcacct
     1081 ggcgcatgtt cgcgccggcg ctcaacgccc gcgcggcgca gcggctgcgc ctggaaaacg
     1141 agctgcgctc ggcgctgaca cgcggcgaac tcgcgctgta ctggcagccc gttgtacgcg
     1201 gctggcggcc cgaccggcgc tcgagcccgg tgcactggcg cggccgcatc gtcggcgcgg
     1261 aagctctggt gcgctggcag catcccgagc ggggactcct cggccccgag cacttcgtgc
     1321 cgctcgcgga ggagtgcggc ctgatccgtg cggtcgacga atgggcgctc gagcgcgcgc
     1381 tgtcgcaggc gggagcctgg cggcggcgct acggaaagaa cctgtggtac gcggtcaacg
     1441 tgtcggcccc gggcctcgcc cagggcgccg aatacctgca gcggctcgag cacgcgctcg
     1501 tcgccaacgg cctcgacggc aagtcggtcg agctcgaagt caccgagcgc gtcgtcatgt
     1561 ccgggctggc ggagaacgtg gcgacgctcg aggcgatcgg caagctcggc gtgcgcttcg
     1621 ccatcgacga cttcggcacc ggctactcga gcctcgcgta cctgaggagg ctgccgatcg
     1681 acaagctgaa gatcgaccgc tcgttcctgg gggcgatcga ttcgcacggc gccgacgcgg
     1741 ccatcgtgcg cgccatcacg gcgctcgccg cgacgctcgg catctcggtc gccgccgagg
     1801 gggtcgagac gccggcgcag ctcgagcggc tgctctcgct cggctgcgag gaatggcagg
     1861 ggcactactt cagcccgccg ctcgacaccg cgggcctgga ggagctcctg tccgagtcgc
     1921 tgtcggcgcg caccgcgcgc agctcggaag cctgaatcag tccctcggcc gcggcgtgcg
     1981 ccgccgcctg ctcggcttcc ccggcgagca cgagctggca gccggttttt gaaacttcgt
     2041 tgatgagaga ttcgagcgcc agcaagcggg ccggatcctg gttgaccgat cttatgtaga
     2101 tggcgcggat gcgctcgggg tggcgctgca ccaccgcgct gtagatctcg ggatcctgct
     2161 cgccgctgtc gccgctcagg atgaagccga gcttcggata cgcggcgagc acctcttcga
     2221 tcgcctttcg cttgtgctcc ttctccgggc gcaggccgaa gtcgcgcagc agcagggggc
     2281 cgatagggag ctgctggacc tcaaggtact cgagcagggg cgcatacagg ttccacgcgc
     2341 tcttcgagat gtagaaaaaa ggattcacgc ccgcatgcag cgcgcggtag aaggccgcta
     2401 cgccgggaaa cggcttgcgc gtacgcgcgt tcgtcagcgc cgcggtaaga atcatgcgca
     2461 ccttgctgcc aacctgactc gcgacgatgg tgtcgtcgat gtcgctgatc acgccgaagc
     2521 gcgcgcgccg cgaaggaacc atgattcttg ccacggcggg cgcagccccc ggccggtcga
     2581 tcaactcgag gctgacttcc tgccatgcac cggggccgag gcggccgcgc ggggcgatct
     2641 cgagccggaa gtagccctcc tggtcggtga caccctgcgt ctcgcggccg aagaagcgcg
     2701 cgcgcagccg cgcgccgggc acctcgtcgg attccaggcg cttccagaag cgcacgaggt
     2761 tgcgccagcg gctgtcgctg tccgtggccg gcgtgaagtt ttcatcctcc agcacccggc
     2821 cgcacatcac gagtcgctcg cgcgtgccgt acccgaggta cggcatgatg attactttat
     2881 cggggtattc tgatcgagga ccatcagcca tcggtcggct tttttcctga acaccagcgt
     2941 gagaaggctc ggctgctccg attcaccgtc ccggtacagc aggcgcagca ccgccagcgc
     3001 catctcggcc gtttcgtaga tcaccgcagg cgccacgtcg agcgtccagc cgggcatgtc
     3061 gaaccaggcg cggtgcgcgt ccaggaattc gcgcggcgtg cgcgcgagct tgccgtcggc
     3121 gccgatgagc acgagctcgt cgtcggccac ggtggcggcg agcgcggcga ggtcgcggtc
     3181 gcggatcgcc gcgaggtggc gctcgagcgc cttgcggaac cctaggcccg caggacgagc
     3241 gccgaaagca gccacgacac gaggctgaac acgatggcgc cgagcacgcc ggcccagaag
     3301 ccgccgacgt ggaagccctc gagccagttg cccaccatcc agaagagcag cccgttcagc
     3361 acgaagatga aaaggccgag cgtgaggatc gtcaccggca gcgtgagcag cacgagcagc
     3421 gggcgcacca ccgtgttgac caggccgagg attagcgccg cgacgagcgc ggagccgaag
     3481 ctcgccacgc tgatcgacgg catgaggtag gcgaccgcga tcagcgcgac ggcgttgatc
     3541 agccagacga ccaggagctt caggatcacg gctgcgcgca gtagaacagg ttggtcaggc
     3601 tcctgatatc caggcgctcg aaccgggtgt agcccgcacg gcgcaccagg gcctcggtgg
     3661 ccgccgggcc gaggcaggtt ccgagtcccg gcccgccgcg cgcaagcgac tgcgtcatgc
     3721 agtggaagag gctgaagccg taaaacgtcg ctgccaccga gttacggttg tcctcgagct
     3781 ggtcggccgc cttcggctcc acgatgaaga gcgtgccgtc ggccttgagg agcgaacgaa
     3841 tcctttcgag ggtcttctcg ggctcggcca ggtcgtgcag cgcgtcgcag agcgtgacga
     3901 agtcgaaacc ggattgccta tcgacggaaa agacgtcgcc cgcgacgaac tcgatccgcg
     3961 aatctcccgc cgacgccctg gcgcggcgaa tcgactcggc gtcggggtcg atgccgatga
     4021 tctcggcctg cgggaacgcc cgggcgagtg cgatgcatac gcgccccgag ccgcagccca
     4081 cgtcgagcgc gcggccgccg gcgcgcaggc gctcggcagc ctgcggcatc gacttcatcc
     4141 agtagtcggc gaagcgcgcg tcgtactgcc cgccattgat gagctcgagc gcctccacgc
     4201 attccgggcc gaacgcttcg aagccgacgc cgccgccggc ggcgaaggcg cgcgccacat
     4261 cgggcgcgac gcgcaggagc ggcggcacca tggcgaacag gccgccgacg aaatggtccg
     4321 tttcgtccga ggcgagaaag taggcctgct cctcgggaag cgagtacgtt tgcgccgagg
     4381 ggtcgtagtc gaggtagccg gcggccacca ttcctttgag ccattcttcg acatagcggg
     4441 gctgcagcct ggagcgcttt gccacttcag acgccgtcat ggcgcccttt cccgccatct
     4501 cgcgcaacaa accggttttc acccccacgt acaccatgcc cgccgccatg gcgcccgcca
     4561 tgtcgcggaa taccttgtcg agcgcctgcc tcgccttgtc tttgtctact cccatagccc
     4621 cttcggcagc ggcaggccgg cgtagtcggc ctcggtcagc ggagcttcgg gctcgatgcc
     4681 aaggcgctcg agcaccgcga tgatctggcg tgcatgctgc gccgagtgcc aggtgcagcg
     4741 ctcgagcagc tcgtgcagcg gccgcgcgcc gtaataggta ttcaccgtct ggcggcagga
     4801 cttgtcggga agcgctgccc accagcgctc gagccggctc tcgatgcgcc tgccgtactc
     4861 gcggatcttc tcggtggaga ggacgtcggc gggcggcggc gcgttgtaga tcgttgtgag
     4921 atcgccgacg ccgtcctcga ccgcctgcag gaacgcgtcg gggatctggt agatgtggta
     4981 cgcgagatcg cggatgctgc ggtcgcggct ctcgaccgcg cgctcgcgca ggcgcgcagc
     5041 cggcagctgg cgcgcgtggc gctgcgccgc gcgcagcacg acgagccatt tggccacgag
     5101 ctcgacggga gacaggcgat cctgcaggaa ctccctgccg atgaagcgcg acacgtcctc
     5161 gagcgcctgc gcgaacacga actcgccgcc gcgcacgacc accgggacgg tcttgacgcc
     5221 gaacgcgagc agctcgcgca tcgcctcggg gcgcgccgag acgtcgatcg actcgaacgg
     5281 cacaccctgc tgcgaaaggt actccttcac cttgacgcag ctcgagcagc cgggacgcca
     5341 gtacaccttg agctcgggat tcatgggacg acgctgactc ctttcacgcc gtgcttctcg
     5401 atcgtgctgg cgacgaggcc cgatcgcttg atgtcctcga tgaactcgct caggtatcgt
     5461 gcacccgcgg tacgcgcctt tgcggtacct gcggcctgct gaacggagct cacctggccc
     5521 tcgagcacgc gcgagcccgg cagcttttcg gcgtcggaaa cgagacgcgg cttcaggccc
     5581 gccagagcct cgagtgtctc tttcaggaag agctcacagg aggcgtcgat gccttccgcg
     5641 cgaacgagct tcgcgtgctt cagcgtgcgg ctcaagtaga ggtcatacgc gctgcgcgcc
     5701 gagacggcga tgcgcacgcc ctcgcggtcc acgtcggcga tgctgcggat cggcgagccg
     5761 gccggcacga ggaaggtgac cgggatttcg agataagcct tggtgaaggc gatgtcggcc
     5821 gcgcgctgcg gctcggcgcc caggaagccg acgtcgcact tgccgtcgcg cacggcgtcg
     5881 gcgagctgcc cggcctgcgc gaacttcacg aacttgaccg gcacgccgag ccggctgccg
     5941 agcgcgcgcc cgaggtcggg ggcgatgccg cgcggcgtgc cgtcgggcgc gtccttcagc
     6001 acgaggagga agttgccgta gttgagcccc acgcgcagcg tgccatgcgg agcaagctcg
     6061 gcgcgcgcag cggccgggat ggcggagggt gcggtcattg ccagcgatag cggtgggtga
     6121 gctgggcgct catcgagcgc aggattctgc atgaatcata ggcgaacctg acctacatat
     6181 gcgctcgcgc cagaaattgc ggtcttgcgc gtgttcacct gcgcggctcg acgagcggca
     6241 cgaagtcgta ctcgccgatt ccctgcaaca cgaggaacgt cgccgagctc tcgccggcgt
     6301 tggtcacgag gtgcgggcgg cgggcgcgcg cggtgtaggt atcgccgggc ctgagccgca
     6361 cttcctcctt cgggtcctgg aggaagatgc gcagctcgcc ttccagcaca tagaacgtgt
     6421 cctgcacgtt gctgtggtag tgccacggga ctttctgcgt cttcgagatc tgcagctcgg
     6481 tgatgcgaaa gccggggcgc gccgcgtgct ccgcgcggcg ctccacctcg tagaggggac
     6541 ggtcgtcctt caccgccttg ttcatagctc gcccagtcct ttgaaaaacg tgtagcagaa
     6601 gacgccgtcg gcttcggcgg tgcggtagag cgcgcggatg ccttcgtcga agcgcgcggg
     6661 cgaaatgagg ccgttcgcga tcgccttttc gcgcacgccc tcgatcatcg cggtgaaggt
     6721 cttgcgcgtg aagccttcca cgagctgcgg gcggctcgag tccacgtaca ccatgcgcgg
     6781 cgagacgcgc acgttgcgca gtcctgcctg aacgaggagg ggatagagct cgcggccgat
     6841 gttcgcgttg ccgccggcgg cacgctggag cgccgcctgg cagccgatcg cgtcgtaggc
     6901 cgccgcgctg tccgggtgga agtaggtcga gccgtggtcg ccttcgatca ccgtcacggt
     6961 tcctcccggc ctcaccaggg ctttgaggcg ctcgagcgcc gcgagcggcc gcgaaaggtg
     7021 ctcgagcacg aagcatacga acacgtggtc gaacgagcgc ggctcgaagg gcagggcgaa
     7081 gatatcggcc tgctggaagc gcacgttcgc cacgccggcc gcttctacct tggccttcgc
     7141 cgcggtgagc gaggcggcgg cgatgtcgac cgacacgatg cgcgcgcccg ggctgcggcg
     7201 cgccagcgtg acggtctggg cgccgacgcc gcagcccgct tcgagcaccg tgcttccgtc
     7261 cgggtaggcg ctgtccgagt gcaagagctc caccagcgcg tccgcctggt cctgcaggcg
     7321 cacgttcgcg cgccggtcgt agccgtggac gtactcgcgc ttcaccaact gcagtctatc
     7381 gaaaacggcg tcattacctt tttcccgaga acggggccag gagtctcccc caagcgcctt
     7441 tttcaagcga gctctgccgc gagttcggag aaatccgcga tgacgatatc cggctggtgc
     7501 ggcgtctggc cgaagggtcg cttgcgccga tcgatgaacg cggtgcgcat gccgtagcac
     7561 ttcgcaccga tgcagtcgaa cgcatggttg gcgacgaaga gcaggctgga gcgcccctcg
     7621 ccgatgatct cctcggcctt cgcataggtc ttccagtgcg gcttgaaata ccccgcttcc
     7681 tgcaccgaga tgacgtggtc gaaccgaaag ccgatgtgcg gccccgcggc ctccagcatg
     7741 tcgcggtcgc cgttggacag gatggcgagc ctgtagcctg ccgagcgaag cttttcaaga
     7801 gcggcgacca cgtcgggaaa gggcttgagc ttctcgatct cgcccaccag ccagcgcacc
     7861 tcgcgctcgg tataggagat gtggcagcgg tccatgacgt acgaaacggc acgatgcccg
     7921 atctcgcggt acggcgtgtg cccgcgatcg cagagcgcgt cgatcatgga gttctcgaaa
     7981 tgcgtgcgcc gccaccaggt gacgaagctg ctcggctcgc cgtcccagcc ctttcgcttc
     8041 aagaaggcag tgacggcttc ggtcaggccc ttttgcatgt cgacgatcgt cccgtactgg
     8101 tcgaacacca gaaccttgat ttctcgcttg agaacgccgc ataacgggtg gatcacatgg
     8161 gccccggggc gtgaggaaga cgggtcgttg tcactgctgt ggcgagggct tcacctggac
     8221 ggggctgacc acgaacctgc gggcttcctt catgagctcc tccagcgcga gctgcagccc
     8281 gccggcgaag tctcccttgc ggaaggcggg caccatcaca gtatcgatga tcgactgcgc
     8341 cttggcattg gaaatgtagc tttccatccc cgaaccgagc tcgatgcgga tgcgcctttc
     8401 cttcatcgcc agggcgacca
//
LOCUS       DASUNT010000447         2476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_852195, whole genome shotgun sequence.
ACCESSION   DASUNT010000447 DASUNT010000000
VERSION     DASUNT010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852195"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VFX94_09060"
     CDS             <1..869
                     /locus_tag="VFX94_09060"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX5768369.1"
                     /translation="FKAGTLIDVAGPALGTLAVFGALLGATLAEADRQRRLLREAQAR
                     VAGELEAARRIQMGLLPSPRRLFAGEARFSLEATLEPARVVGGDFYDCFMVDAHRLFF
                     TVADVSGKGLPASLFMALAKSLLKSIALRTGADPGTILAQASAEIARDNPESFFVTAF
                     AGTLDARSGLLEFCNAGHEPPYLCRAAQRPERLEHQGGPPLCVIEGYAYSSARRTLGR
                     GESLCVLTDGVTEAMDRAGALYGRARLLQLLEKADDPLATLSAVREDVRRFTAGTEQV
                     DDLTLLCVRFNG"
     gene            complement(866..1489)
                     /locus_tag="VFX94_09065"
     CDS             complement(866..1489)
                     /locus_tag="VFX94_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5768370.1"
                     /translation="MVAVLAIIALIVVANQLSVPPAPAAAPVAPAAAAELVVVLPSAD
                     GHTGTVVVDSRGERTILNQPYAANRMGRNSGPTRLSEQEVKSAFGAALGSIPARPASF
                     LLYFVTGTDQLTEESGAELERMLMELRRRPAPDILVIGHTDTVGGDADNDRLSLLRAE
                     RVRASLVAQGIAPGRIHASGRGEREPIVQTGDGVDEPRNRRVEINVR"
     gene            complement(1493..1921)
                     /locus_tag="VFX94_09070"
     CDS             complement(1493..1921)
                     /locus_tag="VFX94_09070"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX5768371.1"
                     /translation="MVILAVAAFAAASHASDVGQIKNLSGAVHVERDGQRLAAAPGMG
                     VRQADVLITGADGAVGVTFLDNSLLSLGPSSTLAIDRYAFDSTTHDGQFDASLKRGTL
                     AVVSGRMVKRSPESMRVRTPSAIMGVRGTEFVVKVDEPVR"
     gene            2020..2412
                     /locus_tag="VFX94_09075"
     CDS             2020..2412
                     /locus_tag="VFX94_09075"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5768372.1"
                     /translation="MFPASLEQLNPLRAFLEGFCAQAGIEREACLRLNLLLEELFTNT
                     VRHGHRGNCDAPVWICLAADSDRRVTITYEDTAPPFNPYARSSPDTTQEIGKLRGRGV
                     LLLQRLAASGDYAYLYGRNRIRLAFNPQ"
BASE COUNT          382 a          873 c          828 g          393 t
ORIGIN      
        1 cgttcaaagc ggggaccttg atcgacgtgg cagggcctgc gctcggcacg ctggcggtat
       61 tcggcgcctt gctcggcgcc acgctggccg aagccgaccg gcagcggcgc ttgctgcgcg
      121 aagcgcaggc gcgcgtcgcc ggcgagctgg aggcggcgcg ccgcattcag atgggcctgc
      181 taccctcgcc ccggcgcctc ttcgccggcg aagcccgctt ctcgctggag gccacgctcg
      241 agccggcgcg cgtcgtgggg ggcgacttct acgattgctt catggtcgac gcgcaccgcc
      301 tgttcttcac ggtggcggat gtctccggca aggggctgcc ggcgagcctg ttcatggcgc
      361 tcgcaaagtc gcttttgaag agcatcgcac tgcgcaccgg cgcggacccg ggcacgatcc
      421 tcgcccaggc gagcgccgag atcgcgcgcg acaaccccga gagcttcttc gtcaccgcgt
      481 tcgccggcac cctcgacgcc cgctcgggct tgctcgagtt ctgcaacgcc gggcacgagc
      541 caccatacct ctgcagggcg gcgcagcgcc ccgagcgcct ggagcaccaa ggggggccgc
      601 cgctatgcgt gatcgaaggg tatgcgtact ccagcgcgcg ccgaacgctc gggcgcggcg
      661 agtcgctgtg cgtgctcacc gacggggtca ccgaggccat ggatcgcgcc ggggcgcttt
      721 acggccgggc gcgtctgctg cagctgctcg agaaggcgga cgatccgctc gccacgctca
      781 gcgccgtgcg cgaggatgtg cgccgcttca ccgcaggcac cgagcaggtc gacgacctga
      841 cgctcctctg cgtgcgattc aacggctagc gaacgttgat ctcgacacgg cggttgcgcg
      901 gctcgtctac gccgtcgccg gtctgcacga tcggctcgcg ctcgccgcgt cccgacgcat
      961 ggatccggcc gggagcaata ccctgggcga cgaggcttgc gcgcacgcgc tccgcccgca
     1021 gcagcgaaag ccggtcattg tcggcgtcgc cgccgacggt atcggtatgc ccgattacaa
     1081 gtatgtccgg cgcgggacgc cggcgcagct ccatgagcat gcgctcgagc tcggccccgg
     1141 actcctcggt gagctgatcg gtgccggtca cgaaatagag caggaaggag gccgggcgcg
     1201 ccggaatcga ccccagcgcc gcgccgaacg cgcttttgac ttcctgctcc gaaaggcgcg
     1261 tcggaccgct gttgcgcccc atgcggttcg ccgcgtacgg ctgattgaga atggtgcgct
     1321 cgcctcggct atccaccacc accgtcccgg tatggccgtc cgcgctgggc agcaccacca
     1381 caagctccgc cgcagcggcc ggggccactg gcgcagcggc aggtgccggc ggaacggaaa
     1441 gctggttcgc aacgacgatc agcgcaatga tggcgaggac cgccaccatg cgctaccgca
     1501 ccggctcgtc gaccttgact acgaattcgg taccgcgcac ccccatgatg gccgacggcg
     1561 tgcgaacgcg catcgattct ggcgagcgct tcaccatccg gccggagacc accgccagcg
     1621 ttccccgctt cagcgacgcg tcgaattggc cgtcgtgcgt ggtgctgtcg aaggcgtagc
     1681 ggtcgatggc cagcgtgctg ctcgggccga gcgatagcag gctgttgtcc aggaaggtga
     1741 cgccaacggc gccgtcagcg ccggtgatca gcacatcggc ctgcctcacg cccatgccgg
     1801 gcgcggcggc gaggcgctgc ccgtcgcgct cgacgtgcac ggcgccgctc aggttcttga
     1861 tctggccgac gtcgctcgca tgggatgctg ccgcgaatgc cgctaccgca aggatgacca
     1921 cccaacgcat cgtgacctcc ctcaggtccc ctgatgcgcg attctataat gcgctacccc
     1981 tgagctgtgt ggacaatgcc cgtggtctta cgaactacga tgttcccggc aagcctcgag
     2041 cagctgaatc ccctgcgcgc atttctcgag ggattctgcg cccaggcggg aatcgagcga
     2101 gaagcctgcc tgcggctgaa cctcctgctc gaggagttgt tcacgaacac ggtgcgtcac
     2161 ggccaccgcg gcaactgcga tgccccggtg tggatctgcc ttgccgcgga cagcgaccgg
     2221 cgggtgacga tcacgtacga ggacactgcg ccgccgttca atccctacgc gcgctcctcg
     2281 cccgatacca cgcaggagat cggaaagttg cgcgggcgcg gcgtgcttct cctgcagagg
     2341 ctggcggcga gcggcgatta cgcgtacctg tacggccgca atcgcatccg cctcgcattc
     2401 aatccgcaat agctcggcgc gttctacgca gcgcccctgg tgagcagacc gacgactttc
     2461 agcaaccgct cgacgc
//
LOCUS       DASUNT010000448         2067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_63285, whole genome shotgun sequence.
ACCESSION   DASUNT010000448 DASUNT010000000
VERSION     DASUNT010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63285"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VFX94_09080"
     CDS             <1..741
                     /locus_tag="VFX94_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase"
                     /protein_id="HEX5768373.1"
                     /translation="RVPQIEVAVGDNAAALVFRHLEALQDADRALLEQFAESHAVHVW
                     LQPGGPETAHPFHPAASELFYELPEFGARIAFRPTDFTQVNHAVNRALVARAVQLLDP
                     QPGERVADLFCGLGNFSLPLAVKGGSVVGFEGNAALVKRAQANAAANGLLAQFEVMNL
                     LQPNLDPFAPFEKLLLDPPREGAVEIVKSLPQHWPRRIVYVSCDPATLARDAGILVHT
                     KGFRLAAAGVVNMFPHTAHVESIAAFER"
     gene            complement(823..1557)
                     /locus_tag="VFX94_09085"
     CDS             complement(823..1557)
                     /locus_tag="VFX94_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEX5768374.1"
                     /translation="MQPELKPVRSDDRGGFGRGTTYQPAQDIAVSAEGRKVLRNTYML
                     LALTMVPTVIGAWIGMSTASIVTSSPIISTLVMLAAVIGLQFAIAANRNSGIGVALLL
                     LMTGLLGWWLGPILNVAFSMKNGMALVGYAAVGTGIIFFAMGAAAQAIKRDLSFMGKF
                     LFVGMIALLVAMIANMFRQIPALALTISTLVIVVFAMFLLYDLQRIMRGGETNYVMAT
                     TGVYMSLFNIFANLLHLLLALGGERE"
     gene            complement(1860..>2067)
                     /locus_tag="VFX94_09090"
     CDS             complement(1860..>2067)
                     /locus_tag="VFX94_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768375.1"
                     /translation="RSWRCCAATRRDRPKRAEAFSGVPINAVAARLGHSTPVITLKVY
                     GHVLRRAEDQAVSVAGKLLEGALP"
BASE COUNT          365 a          682 c          656 g          364 t
ORIGIN      
        1 cgcgtgccgc agatcgaggt cgcggtcggc gacaatgccg cggctcttgt tttccgccac
       61 cttgaagcgc tgcaggatgc cgatcgagcg cttctcgagc aattcgccga aagccatgcc
      121 gtccatgtgt ggctgcagcc cggcggcccg gaaaccgccc acccgttcca tccggcggcg
      181 agcgagctct tctacgagct gccggagttc ggcgcgcgca tcgcctttcg cccgacggac
      241 ttcacgcagg tgaaccacgc cgtgaaccgc gcgctcgtcg cgcgcgcggt gcagctcctc
      301 gacccgcagc ccggcgagcg cgtcgccgac cttttctgcg ggctgggtaa tttctcgttg
      361 ccgctcgccg tgaagggcgg ctcggtggtg ggattcgaag gcaatgccgc gctggtgaaa
      421 cgagcgcagg cgaatgccgc ggccaatggc ctcctcgcgc aattcgaggt gatgaacctc
      481 ctccagccca atctcgaccc cttcgcgccc ttcgaaaagc tgctgcttga cccgccgcgc
      541 gagggcgcgg tcgagatcgt caagtcgctg ccgcagcact ggccgcggcg catcgtctac
      601 gtgtcgtgcg acccggcgac gctcgcacgc gatgccggaa tcctcgttca caccaaagga
      661 ttccgccttg ccgcggccgg cgtcgtgaac atgtttccgc ataccgcgca cgtggaaagc
      721 attgcggcgt tcgagcgctg atgcgcgcgc gagtgttctc gagcggaaaa acaaagggcg
      781 ccgtaggcgc cctcgatttg cactactgcg gctgcttcgt ccttattctc tctcgccccc
      841 gagcgcgagg agcaggtgga gcaggttggc gaagatgttg aacaggctca tgtagacgcc
      901 ggtggtggcc atcacgtagt tcgtctcccc gccgcgcatg atgcgctgca ggtcgtagag
      961 caggaacatg gcaaacacca cgatcacgag cgtggatatc gtcagcgcga gcgctgggat
     1021 ctgtcggaac atgttcgcga tcatcgcgac aaggagcgca atcatgccga cgaagaggaa
     1081 cttccccatg aagctcagat cgcgcttgat ggcctgggca gccgcgccca tggcgaagaa
     1141 aatgatgcct gtgcccacgg cggcgtagcc gacgagcgcc atgccgttct tcatggagaa
     1201 cgcgacattc aggatcggcc cgagccacca gccgaggaga ccggtcatca gcagcagcag
     1261 cgcgacgccg atgccgctgt tgcggttggc cgcgatggcg aactgcaggc cgataaccgc
     1321 ggccagcatg accagcgtgc tgatgatcgg gctcgacgtg acgatgctcg ccgtcgacat
     1381 cccgatccag gcgccgatca ccgtcggcac catggtcagc gcgagcagca tgtaggtgtt
     1441 gcgcaggacc tttcggcctt ctgcgctgac agcgatgtcc tgggcaggct ggtacgtcgt
     1501 gccgcgcccg aagccaccac gatcgtcgga gcgcacgggt ttcagttccg gttgcatccc
     1561 aaatcctcct ttatgtagcc ggtaagacta ccaaagtgcg ccgaagtttc aacgcttccc
     1621 accgcgcacg aaggctaaaa tgcggccgtt acagggtttt ttcaagccgg agcgttggca
     1681 gagcgctcga atgcggcggt cttgaaatcc ttcagcggaa tgccttcgcg tgccgcccct
     1741 acggcagcgt gctgatttca ctctgagaac gggtagcagt cagggtgcgt cgtgctgcgc
     1801 cgttccgccc cgtaccgcgc tgttacaggc gcagcagaat ccaaaccgga atccaaggct
     1861 cacggcagcg ccccctcgag cagcttgcca gctacgctga cggcctgatc ctcagcgcgg
     1921 cgcaggacgt gcccgtagac cttcagcgtg atgacgggcg tcgagtgccc gaggcgcgcc
     1981 gccacagcgt tgatcggcac cccgctgaaa gcttcagccc gtttcggcct gtcgcgcctt
     2041 gtggccgcgc agcagcgcca cgagcgc
//
LOCUS       DASUNT010000449         2988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1027277, whole genome shotgun sequence.
ACCESSION   DASUNT010000449 DASUNT010000000
VERSION     DASUNT010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027277"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="VFX94_09095"
     CDS             complement(<1..877)
                     /locus_tag="VFX94_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5768376.1"
                     /translation="MKPLTSVKRSYFRQPVAIALGAASLAVLVGMNAMAFLRGLDAPW
                     LLGAVIISDMLVIGTGIVVAAREVLLAENRTEASEAQLAAIVDSAMDAIISVNESQNI
                     VLFNRAAEQVFGVRREQALGSPLDRFLPARFRTAHRGHIQHFGTTGVTSRRMGDITTL
                     WALRADGEEFPIEASISQSGAPGARHYTVILRDITRRKQFEDALKASQRELRELSARV
                     LEAREEEKAHIARELHDELGQLLTALKMDLGWLRERLPADTELTARAAEMGALLDRTV
                     GSTRRISADLRPLMLD"
     gene            902..2293
                     /gene="hemN"
                     /locus_tag="VFX94_09100"
     CDS             902..2293
                     /gene="hemN"
                     /locus_tag="VFX94_09100"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEX5768377.1"
                     /translation="MPYPASELVIDPVLIRKYDVSGPRYTSYPTADRFVEAFSEPELE
                     QWLGKRNIGGFSQPLALYVHLPFCDTLCYYCGCNKVVTRDHGRSAKYIKYLAKELTLL
                     APHLGSRSTIEQLHWGGGTPNFLSREQMSELVGVIEASFERGKKTEYAIEVDPRSAEP
                     GRMAFLASLGFTRLSIGVQDFDAAVQKAVHRIQSVETTRRVMEEARAHRFKSVNLDLI
                     YGLPRQSLDSFSRTLDEVLALEPERIALYNYAHLPKVFKPQRRIVEAELPSPELKLEL
                     MTLAIGRLTRAGYLYIGMDHFARPEDELAVAQNLGKLARSFQGYSTHSGDLIGLGLSS
                     IGQVGPTYYQNVKELERYYAALDAGRLPVLRGVELTTDDLARRAVIQALMCQFRVSIE
                     SIELSYLIDFQRHFARELHMLRKLAADGLVELEPDWIVVTPKGRLLVRAVAMIFDRYL
                     RESQERARYSKVI"
     gene            2290..2988
                     /locus_tag="VFX94_09105"
     CDS             2290..2988
                     /locus_tag="VFX94_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5768378.1"
                     /translation="MNELALPLALFAGGLASGLHCAGMCGGIAAGFSLLQKEAVWKRQ
                     LAFNAGRITSYAGAGAIAGALGSAAAYAAAALPAQSFLYFFSSAFVLLAGMHVWGLPL
                     PLSALETIGVPLWRRIQPLAARLLPARSVPQSYAAGLAWGWLPCGLVYGALAAAVFAG
                     GAAQGAVAMAAFGAGTLPWLFIAGVAAARLRRWSGLANLRRLGGALLIALGGFGVAQA
                     SGLGESLRQTLACF"
BASE COUNT          452 a         1023 c         1001 g          512 t
ORIGIN      
        1 catcgagcat gagcggccgc aggtcggccg aaatgcgccg cgtcgagccg accgtgcggt
       61 cgaggagcgc gcccatctcc gcggcgcgcg ccgtgagctc ggtatccgcg ggcagccgct
      121 cgcgcagcca cccgagatcc atcttgagcg cggtgaggag ctggccaagc tcgtcgtgca
      181 gctcgcgggc gatgtgcgct ttttcctcct cgcgggcttc gagcacgcgc gccgaaagct
      241 cgcgcagctc gcgctgcgag gccttgagcg cgtcctcgaa ctgcttgcgc cgggtgatgt
      301 cgcgcaggat cacggtgtag tggcgggcgc cgggcgcgcc cgactgcgaa atcgacgcct
      361 cgatgggaaa ttcctcgccg tccgcgcgca gcgcccacag cgtagtgatg tcgcccatgc
      421 gccggctggt gaccccggtg gtgccgaaat gctggatgtg gccgcgatga gccgtgcgaa
      481 agcgcgccgg caggaagcgg tcgagcgggc tgccgagcgc ctgctcgcgc cgcacgccga
      541 acacctgctc ggcggcgcgg ttgaagagca cgatgttctg gctctcgttg accgagatga
      601 tcgcgtccat cgccgagtcg acgatcgcag cgagttgcgc ctcgctcgct tcggtgcggt
      661 tctcggcgag caatacttcg cgcgcggcga ctacgatgcc ggtgccaatc accagcatgt
      721 cggaaatgat cacggctccg aggagccatg gcgcgtcgag cccgcgcagg aaagccatcg
      781 cattcatacc gaccaggacg gcaagcgatg ccgcgcccag cgcgatcgcc accggttggc
      841 ggaagtagct ccgtttgacc gaggtcaaag gtttcactcc ccctagcttc atagtccccc
      901 aatgccctat cccgcgtcgg aactggtcat cgacccggtg ctgattcgca agtacgacgt
      961 gagcggcccg cgctacacct cgtaccccac tgcggatcgc ttcgtcgagg cctttagcga
     1021 gccggagctg gagcaatggc tcggcaagcg caacatcggc ggcttcagcc agccgctcgc
     1081 gctctatgtc cacctgccgt tctgcgacac gctttgctac tactgcggct gcaacaaggt
     1141 cgtgacccgc gaccacggca gatcggccaa gtatatcaag tacttggcca aggaattgac
     1201 gctccttgcg ccgcatctgg gcagccgctc gacgatcgag cagctgcact ggggcggcgg
     1261 cacgccgaac ttcctttcgc gtgagcagat gagcgagctc gtcggcgtca ttgaagcgag
     1321 cttcgagcgc ggcaagaaga ccgagtacgc aatcgaggtc gacccccgca gcgccgagcc
     1381 gggccgcatg gccttccttg cctcgctcgg cttcacgcgg ctgtcgatcg gcgtgcagga
     1441 tttcgatgct gcggtacaga aggcggtgca ccgcatccag agcgtcgaga cgacccgccg
     1501 cgtcatggaa gaggcgcgcg cgcatcgttt caagtcggtc aacctcgacc tgatctacgg
     1561 gttgccgcgc cagagcctcg acagcttcag ccgcacgctc gacgaagtgc tcgcgctcga
     1621 gcccgagcgc atcgcgctct acaactatgc gcacctgccg aaggtcttca agccgcagcg
     1681 gcgcatcgtc gaagcggagc tgccctcgcc ggagctcaag ctcgagctca tgaccctcgc
     1741 catcgggcgc ctgacgcgcg cgggctatct gtacatcggc atggatcatt tcgcgcgccc
     1801 ggaggacgag ctcgccgtcg cgcagaacct gggcaagctc gcacgcagct ttcagggcta
     1861 ttcgacgcac tcgggcgatc tcatcggcct ggggctctcc tcgatcgggc aggtcggccc
     1921 gacctactat cagaacgtga aggagctcga gcgctactac gccgcgctcg atgccggccg
     1981 gctgcccgtg ctgcgcggcg tggagctcac gaccgacgac ctcgcgcggc gcgccgtgat
     2041 ccaggcgctc atgtgccagt tccgggtgtc gatcgagtcg atcgagctct cgtatctcat
     2101 cgacttccag cgccacttcg cccgcgagct gcacatgctg cgaaagctgg ccgcggacgg
     2161 gctggtcgag ctcgagcccg actggatcgt ggtgacgccc aagggccggc tcctggtgcg
     2221 cgcggttgcc atgatcttcg accgctacct gcgcgagtcg caggagcggg cgagatattc
     2281 gaaggtcata tgaacgagct cgcattgccg ctcgcgctct tcgccggcgg gctcgcctcg
     2341 ggcctgcact gcgccggcat gtgcggcggc attgccgccg ggttttcgct gctgcagaaa
     2401 gaggccgtct ggaaacgcca gctcgcgttc aatgcgggcc gcatcacgag ctacgccggc
     2461 gccggcgcca tcgcgggtgc gcttggcagc gcggccgcgt acgccgcggc ggcgctcccc
     2521 gcgcagagtt tcctgtattt cttctccagt gcattcgtcc tgcttgccgg aatgcacgtc
     2581 tggggattgc cgctgccgct ctcggcgctg gaaacaatcg gcgttccgct ctggcggcgg
     2641 atacagccgc tcgccgcgcg cttgcttccc gcacgctcgg tgccgcagtc gtacgccgcc
     2701 ggcctcgcct ggggctggct gccgtgcggc ctcgtctacg gcgcgcttgc cgccgcggtg
     2761 ttcgccggcg gcgcggcgca aggcgcggtt gcaatggcgg cgttcggcgc cgggacgctg
     2821 ccgtggctct tcattgccgg agtggccgcg gcgcggctac gacgatggag cgggctggcg
     2881 aatctgcgac gcctgggcgg agccctcctg atcgcgctcg gcggcttcgg cgttgcgcaa
     2941 gcgagcggcc ttggcgaaag tctccggcaa acgctcgcct gcttctag
//
LOCUS       DASUNT010000450         3294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1027287, whole genome shotgun sequence.
ACCESSION   DASUNT010000450 DASUNT010000000
VERSION     DASUNT010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027287"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VFX94_09110"
     CDS             <1..247
                     /locus_tag="VFX94_09110"
                     /inference="COORDINATES: protein motif:HMM:NF039524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768379.1"
                     /translation="QAGKIPSSVDEMPAHGDALQAARERLNQTLAGVGEQAKQAAQYA
                     DEAVYRNPWTSVGIGFGVGVLVGALIAIAAGSKSSRL"
     gene            288..794
                     /locus_tag="VFX94_09115"
     CDS             288..794
                     /locus_tag="VFX94_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEX5768380.1"
                     /translation="MNISMYQASAPRFANALKNLSAILGKAQAHAEAKNIDPAVMGSL
                     RLIADMFPLSRQVQIACDTAKGAVARLAGLDIPKHEDNEQTIAELKARIAKTLDFLGS
                     VPAGKIDGSEQREVVLKMRSGEHKYTGLQYLMGHAWPNFYFHVPTAYNILRANGVDIG
                     KRDFIGQP"
     gene            complement(873..1298)
                     /locus_tag="VFX94_09120"
     CDS             complement(873..1298)
                     /locus_tag="VFX94_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768381.1"
                     /translation="MRLAVLATIVSALLVACADPVTKEETAGLDYGPKPVHWRKEITS
                     YLSVRLTDPKAAIVEFRTDPQQMYQRKVAFRNMHYGWATCVWVNDKNTSGAYAGFYPM
                     TFFFRNEKIVQVNGGPDDFGIGAQYARSQCEQLGAPFKQ"
     gene            1408..1917
                     /locus_tag="VFX94_09125"
     CDS             1408..1917
                     /locus_tag="VFX94_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768382.1"
                     /translation="MMKQIIAASVLTVAASAAAAADVGVAGAFSKGRVHFGVTAGSGS
                     AFDDDYIVLGIGASYYLLDGLGVGIHYEWWSGGDPDITKLTGSVQYVFHQMQPVKPYL
                     GGFYRRTDIDGLPDLDSVGGRAGIYIGAARNAYIGVGVVYENYLDCNETVYRSCDSTY
                     AEVTFTFAF"
     gene            complement(1924..>3294)
                     /locus_tag="VFX94_09130"
     CDS             complement(1924..>3294)
                     /locus_tag="VFX94_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5768383.1"
                     /translation="SQLNSSRVWRIVQPDTLEGIRAAVRAAQNEDRAVCISGARHAMG
                     GQQFLADGLMIDTRRMSRLLNFDADKGHVEFEAGIQWPQVLAQLAAVQKGRERQWTFA
                     QKQGGADKLTLGGCLASNVHGRGLKLPPFIGDVESFRLLTAKGDVVNCSRTENAELFR
                     LAIGGYGLFGLITSVTLRLIERRKVERVVEVRSVEGLLKAFAERIGEGCLYGEFRYAI
                     DEASEDFLRRGVLACYRPVDPATPVPPGQKQLHERDWVELLTLAHHAKSEAFRRYAGH
                     YVATNGQVYWADEAQMSIYPENYHRALDQRVGATVKATETITEIYCEREALERFMTEV
                     RDYARRDKVDIIHATVRLIEQDRESFLAWARKPYACVLFNVHVEHTTSGLIKAGDLFR
                     RLIDIGLKHGGSYFPSYNRYALRRQVEACFPQMQEFLKLKRKYDPKELFQSEWYRHYK
                     RMFEKN"
BASE COUNT          558 a         1039 c         1110 g          587 t
ORIGIN      
        1 ccaggcaggg aagatcccga gcagcgtgga tgaaatgccg gcgcacggcg acgcgttgca
       61 ggcggcgcgc gagcgcctca accagacgct ggcgggcgtg ggcgagcagg ccaagcaggc
      121 ggcccagtat gccgacgagg cggtttacag gaacccctgg acgtcggtgg gcatcggctt
      181 cggcgtcggc gtgctggtcg gtgccctgat cgctatcgcc gcgggatcga agagctcgcg
      241 gctctagccg cggctacaat cgcgttcctc gaactgcggg gaatgcgatg aacatctcga
      301 tgtaccaggc gagtgcgccg cgcttcgcga acgcgctgaa aaacctttcg gccatcctcg
      361 gcaaagccca ggcgcacgcc gaagcgaaga atatcgaccc ggcggtgatg ggcagcctgc
      421 ggctgatcgc ggacatgttt ccgctgtcgc ggcaagtcca gatcgcctgc gacaccgcca
      481 agggcgcggt ggcccggctt gcggggctcg acattccgaa gcacgaggat aacgagcaaa
      541 cgatcgccga gctgaaggcg cgcatcgcga agacgctcga cttcctcggc tcggtgccgg
      601 cggggaaaat cgacggatcc gagcagcgcg aggtggtgct caagatgcgc tccggcgagc
      661 acaagtacac cgggctgcaa tacctgatgg gccacgcctg gcccaacttc tactttcacg
      721 tgccgaccgc ctacaacatc ctgcgcgcga acggcgtcga tatcgggaag cgcgacttca
      781 tcgggcagcc ttgacgagcg ggcaccgggt cggaaatcgg gtcggaaatc ggggtcagac
      841 cccaaccggc atcggggtgt gcccccagct actcactgtt tgaacggcgc gccgagctgc
      901 tcgcactggc tgcgagcgta ctgggcgccg atgccgaaat cgtcggggcc gccgttcacc
      961 tgcacgatct tctcgttgcg gaagaaaaac gtcatcggat agaaaccggc ataggccccc
     1021 gaggtgttct tgtcgttcac ccagacgcag gtggcccagc cgtaatgcat gttgcggaag
     1081 gcaactttgc gctggtacat ctgctgcggg tcggtgcgga actccacgat ggccgccttg
     1141 gggtcggtca gccgcaccga caggtagctc gtgatctcct ttctccagtg caccggcttc
     1201 gggccgtagt cgagccccgc ggtctcctcc ttggtcaccg ggtcggcgca cgcgacgagg
     1261 agggcggaaa caatcgtggc gaggaccgcg agtcgcattt ggagttctcc ctgtggcaaa
     1321 aaagcattat gcatcgcaat gcggccgggt cggtgcggcg tctcgcgccg cggtagacga
     1381 aggacatgcg tgcgacaatg ggacaccatg atgaaacaaa taattgcggc aagcgtgctc
     1441 acggtggcgg catcggcggc tgctgcggcg gatgtgggcg tggctggcgc cttcagcaag
     1501 ggccgcgtgc acttcggcgt gactgccggc agcggctcgg ctttcgacga cgactatatc
     1561 gtcttgggca tcggcgcgag ctactacctg ctggacggtt tgggcgtcgg tattcactac
     1621 gagtggtggt cgggcggcga tcccgatatc acaaagttga ccggctcggt gcagtatgtc
     1681 ttccaccaaa tgcagccggt caagccttac ctggggggat tttaccggcg caccgacatc
     1741 gacggcttgc cggatctcga ttcggtcgga ggccgggccg ggatctacat cggcgcggcg
     1801 cgcaatgcct acatcggcgt gggcgtggtg tacgagaact acctcgattg caacgagacc
     1861 gtttaccgtt cctgtgacag cacctatgcc gaagtgacgt tcaccttcgc gttctagcgc
     1921 ttcctagttc ttctcgaaca tcctcttgta gtgccggtac cactcgctct ggaagagctc
     1981 cttcgggtcg tatttccgct tgagcttgag aaactcctgc atctgcggaa agcacgcttc
     2041 gacctggcgc cgtagcgcgt agcggttgta gctcggaaag tagctgccgc cgtgcttgag
     2101 cccgatgtcg atcaggcggc gaaagaggtc gcccgccttg atcagcccgc tcgtggtgtg
     2161 ctcgacgtgg acgttgaaga ggacgcaggc gtagggcttg cgcgcccagg cgagaaagct
     2221 ctcccggtcc tgctcgatca gccgcaccgt ggcgtggatg atgtcgacct tgtcgcgccg
     2281 cgcgtagtcg cgcacctccg tcatgaagcg ctcgagcgct tcgcgctcgc agtagatctc
     2341 ggtgatcgtc tcggtcgcct tcacggtcgc gccgacgcgc tggtcgagcg cgcggtggta
     2401 gttctccggg tagatgctca tctgcgcctc gtcggcccag tacacctggc cgttggtggc
     2461 gacgtagtgc ccggcgtagc ggcgaaacgc ctcgctcttc gcgtggtggg cgagcgtgag
     2521 aagctcgacc cagtcgcgct cgtgcagctg cttctgcccc ggcgggaccg gcgtcgccgg
     2581 atcgaccggg cgatagcatg cgagcacgcc gcggcgcagg aagtcctccg aggcttcgtc
     2641 gatcgcgtac cggaactcgc cgtagaggca accttcgccg atccgctcgg cgaaggcctt
     2701 gagcaggcct tcgaccgagc gcacttcgac cacgcgctcg accttgcgcc gctcgatgag
     2761 gcgcaaggtc accgaggtga tcaggccgaa gagaccgtag ccgccgatcg cgaggcggaa
     2821 gagctccgcg ttctcggtgc ggctgcagtt gactacgtcg cccttcgcgg tgagcaggcg
     2881 gaacgattcc acatcgccga tgaacggcgg cagcttcagt ccgcgtccgt gcacgttcga
     2941 cgccaggcag ccgccgagag tcagcttgtc ggccccgccc tgcttctgcg cgaacgtcca
     3001 ctggcgctcg cggcctttct gcacagcggc gagttgcgcg agcacctgcg gccactggat
     3061 gccggcctcg aactcgacgt gtcccttgtc ggcgtcgaag ttgagcaggc ggctcatgcg
     3121 tcgcgtgtcg atcatgaggc cgtcggcgag gaactgctgg ccccccatgg catggcgcgc
     3181 gcccgagatg cacaccgccc ggtcctcgtt ctgcgccgcg cgcacggcgg cacggatgcc
     3241 ctccagggtg tcgggctgga cgatgcgcca gacgcgcgac gaattgagct gcga
//
LOCUS       DASUNT010000451         3444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_589018, whole genome shotgun sequence.
ACCESSION   DASUNT010000451 DASUNT010000000
VERSION     DASUNT010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589018"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VFX94_09135"
     CDS             <1..880
                     /locus_tag="VFX94_09135"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEX5768384.1"
                     /translation="ALSALLFYWRVVPAVVRAISYLLERTMRIGGVVGLSTAANVFVG
                     MVEAPLFVRPYLARVSRGELFAIMAGGMASIAGTVLFLYGSILSRVQPDAVAHLLIAS
                     ILSAPAALVVAFVMVPPVTTTGGDLELRSEASGAMDALTRGTLEGAQLLLNIVAMLVV
                     FAALVALVNLIIAPYTLQGALGWALAPLAWLAGIPWSEARAAGALLGTKTVINELVAY
                     LDLARMSDISERSRVLLTYALCGFANFGSLGIMIGGLATMSPERRAEIVELGIRSIVA
                     GTLATCLTAATVALIL"
     gene            1076..2446
                     /gene="glgA"
                     /locus_tag="VFX94_09140"
     CDS             1076..2446
                     /gene="glgA"
                     /locus_tag="VFX94_09140"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEX5768385.1"
                     /translation="MRVLYVTPECAPLAKAGGLGDVSAALPAALRALGHEVDVLLPAY
                     AGVLAKSRDAVEHDRVHELGFECRLLRHERFLLAECAALYERAGTPYQDAAGEDWPDN
                     ALRFGVFSKVASRLAAGYDVVHCNDWPTALAPVFARGEKTLVTIHNLAFQGNFERAWL
                     ARLELPEELFSMRELEFHGRISFLKGGLVHAGAINTVSTTYAREIQTEEFGCGLDGLL
                     RSRRGALTGILNGIDTAVWDPATDPHLAARYDATSLDRKSANKRELQKRLDLQPGDDP
                     LIGFVGRLTHQKGADLLAAAASEIAALPAQIALLGRGERSIEAALAAAAARHPGRIAV
                     AIRFDEALAHLIEAGADLFVMPSRFEPCGLNQMYSQRYGTPPVVRATGGLADTVQEGE
                     TGFLFESAEASALAAAVRRALAARRDPDRWREIQRAGMQRDFSWSAAARRYAALYSGL
                     SMARRT"
     gene            complement(2376..3353)
                     /locus_tag="VFX94_09145"
     CDS             complement(2376..3353)
                     /locus_tag="VFX94_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX5768386.1"
                     /translation="MNIVAMVLAGGEGKRLYPLTAEEAKPALAFANGYRLVDFVLSNL
                     VNSGISTIYVLAQYKPESLIRHIAAAWSPWSADGEGSVKLLLPRSNTLGGQFKGTADA
                     VYQYLDVVQAHSPDVVAVFAADHVYRMDVRQMVAAHCGRKADVTVSAVRVPLRQASGF
                     GILATTADGRVREFREKPRNAAPLPSDPTRAYASMGNYLFEPQVLEQELHEARRAGRI
                     DFGRDLLPRLCASRGVYAYDFAENRVPGVQQYEEPAYWRDVGTLAALAAARQDAMASP
                     PRFNLVNRRWPIRGGRDAARRPGGQSLKSAAPWKAPSRERHSGERLPTS"
BASE COUNT          507 a         1160 c         1174 g          603 t
ORIGIN      
        1 cgcgctttcg gcgctgcttt tctactggcg cgttgtgccg gcggtggtgc gggcgatttc
       61 atacctgctc gagcgcacga tgcgaatcgg cggcgtggtg gggctctcca ccgcggcgaa
      121 cgtgttcgtg gggatggtgg aggcgccgct tttcgtgcgg ccttaccttg cgcgcgtgtc
      181 gcgcggcgag ctcttcgcca tcatggcggg cggcatggcg tcgatcgccg gcacggtcct
      241 cttcctctat ggcagcattc tcagccgcgt gcagcccgac gcggtggcgc atctgctcat
      301 cgcctcgatc ctctcggcgc ccgctgcgct cgtcgtggcg ttcgtcatgg taccgcccgt
      361 aaccacgacc gggggagatc tcgagctcag atccgaagcc tccggtgcga tggacgcgct
      421 gacccgtggg acgctggaag gcgcgcagct gcttctcaac atcgtcgcga tgctcgtggt
      481 gttcgcagcg cttgtcgcgc tcgtgaacct catcatcgcg ccgtacacgc tgcaaggcgc
      541 gctcggctgg gcgctcgcgc cgcttgcatg gctcgccggc attccgtgga gcgaggcgcg
      601 cgccgccgga gcgctgctcg gcaccaagac cgtaatcaac gagcttgtcg cctacctgga
      661 tctcgcgcgg atgagcgata tttccgagcg cagccgggtg ctgctcactt atgcgctctg
      721 cggcttcgcc aatttcggca gcctcggcat catgatcggc ggcctcgcca ccatgtcgcc
      781 ggagcgtcgc gccgagatcg tcgagctcgg aataaggtcg atcgtggccg gaacgcttgc
      841 tacctgcctg acggcggcaa ccgtggcact catcctttaa tgcccgtgta gtggactgtc
      901 gacaacaggt tcatccgttt ggcgatagtt cggctagggc gcgcgcccta ggatcatgcg
      961 cgaggaagca cgcagcaccg cgttcccccc ttgcatacgc acctccctcc cttcgtgcgt
     1021 atgtaggcga tgaccccggc accccccggg gtcttttttt atctgcagga gaagaatgcg
     1081 cgttctctac gtgacgccgg aatgcgcgcc gctcgcgaag gcgggcgggc tcggcgatgt
     1141 gagcgccgca ctaccggcgg cgctgcgcgc gctcgggcac gaggtcgacg tgctgcttcc
     1201 cgcgtatgcg ggggtgctgg caaagtcgcg tgatgcggtg gagcacgacc gcgtccacga
     1261 acttggcttt gaatgccggc tgctgcggca cgagcggttc ctgctcgcgg agtgcgcggc
     1321 gctctacgag cgcgcgggca cgccttacca ggatgccgca ggcgaggact ggccggacaa
     1381 cgcgctgcgc ttcggcgtct tttcgaaagt tgcgagccgg ctcgccgcgg gctacgacgt
     1441 cgttcactgc aacgactggc ccaccgcgct tgccccggtc ttcgcgcgag gcgaaaagac
     1501 gctcgtcacg atccacaatc tcgccttcca gggcaatttc gagcgcgcgt ggctcgcccg
     1561 gctcgagctg ccggaggagc tcttctcgat gcgcgagctc gagtttcacg gccggatctc
     1621 gttcctcaaa gggggtctcg ttcacgcggg cgcgattaac acggtgagca cgacctacgc
     1681 gcgcgagatc cagaccgagg agttcggctg cgggctggac gggctgcttc gcagccgacg
     1741 cggcgcgctc accggcatct tgaatggcat agacaccgcg gtgtgggatc cggcgacgga
     1801 tccgcatctc gccgcgcgct acgacgcaac gtcgctggac agaaaaagtg cgaacaagcg
     1861 cgaattgcag aagcggctcg accttcagcc gggcgacgat cctctcatcg gcttcgtcgg
     1921 ccgcctgacg caccagaagg gtgcggatct gctcgccgcc gccgcatcag agattgccgc
     1981 gctgccggcg cagatcgcgc tccttggacg cggcgagcgc tcgatcgagg ccgcgctcgc
     2041 cgcggcggcc gcgcgccacc cgggcaggat cgcggtggcc atcaggttcg acgaggcgct
     2101 tgcccatctc atcgaggcgg gcgcggatct ctttgtcatg ccctcgcgct tcgagccctg
     2161 cggactcaac cagatgtaca gccagcgcta cggcacgccg cccgtggtcc gcgccaccgg
     2221 tgggctggca gatacggtgc aggagggcga gactgggttt cttttcgaga gcgccgaggc
     2281 ttccgcgctg gctgcggcag tgcgccgagc gctcgcggcg cggcgcgatc cggaccgatg
     2341 gcgggagatt cagcgcgcgg gaatgcagcg ggacttcagc tggtcggcag ccgctcgccg
     2401 ctatgccgct ctctactcgg ggctttccat ggcgcggcgg acttgagcga ctggccaccc
     2461 gggcgccgcg ccgcgtcgcg cccgccgcgg atcggccagc ggcgatttac caggttgaag
     2521 cgcggcgggc tcgccatcgc gtcctgccgc gcggccgcga gcgcggcgag cgtgccgacg
     2581 tcgcgccagt acgcgggctc ctcgtactgt tgcacgcccg gcacgcggtt ttcggcgaaa
     2641 tcgtaggcgt acaccccccg gcttgcgcac aggcgcggca agaggtcacg cccgaaatcg
     2701 atgcgccccg cgcgccgcgc ctcgtgcagc tcctgctcca gcacctgtgg ctcgaacagg
     2761 tagtttccca tcgaggcata ggcgcgcgtc ggatccgagg gcagcggcgc cgcgttgcgc
     2821 ggcttctcgc ggaactcacg cacccggccg tccgccgtcg tggcgaggat gccgaacccc
     2881 gacgcctgcc tgagcggcac gcggactgcc gagacggtca cgtccgcctt gcgcccgcaa
     2941 tgtgcggcga ccatctggcg cacgtccatg cggtagacat ggtcggcggc gaacaccgcg
     3001 acgacgtcgg gcgaatgcgc ctggacgaca tcgaggtact gatataccgc gtctgcggtc
     3061 cccttgaact gcccgccgag cgtgttcgag cgcggcagca gcagtttgac cgagccctcg
     3121 ccgtcggcgg accacgggga ccacgccgcc gcgatgtgcc ggatcagaga ttcgggcttg
     3181 tactgcgcga gcacgtagat cgtggagatg cccgagttca cgaggttgct cagcacgaaa
     3241 tcgacgaggc gatagccgtt cgcgaaggcg agcgcgggct ttgcctcctc ggcggtgagc
     3301 gggtaaagac gcttgccctc gccgcctgcg agaaccatcg cgacgatgtt cattcctttg
     3361 ggggcctttc tcggtggaat tgctccgaca aacgacgccg caatcatatt tgagcagtag
     3421 cgatcgtcac tatcgcggcg gacc
//
LOCUS       DASUNT010000452         3045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1292100, whole genome shotgun sequence.
ACCESSION   DASUNT010000452 DASUNT010000000
VERSION     DASUNT010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292100"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="VFX94_09150"
     CDS             complement(<1..1099)
                     /locus_tag="VFX94_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HEX5768387.1"
                     /translation="MQVAQDSATALSSLSVGVEERGTSARGAYADYKVIRRNGAVVAF
                     EPAKISVAMTKAFLAVNGSQGAASARIRELVAALTDNVVAALLRRQPQGGTFQIEDIQ
                     DQVELALMRSGEHDVARAYVLYREAWAKARAQEKTAAKGASEIHVVDGSERKPLDRVR
                     LRRLVESACEGLADASAPAILEATLRDLYDGVPMQEVRKSAVLAARALIEKDPSYSYV
                     TARLLLDTIRLEVLGEEASQADMDTRYAGYFPGFIERGIAAELLDPELARFDLERLGR
                     ALKAGRDLHFGYLGLQTLYDRYFLHVGGRRIELPQAFFMRVAMGLALREKDREARAIE
                     FYDVLSSFDFMSSTPTLFNSGTLRSQLSSCYL"
     gene            complement(1427..1942)
                     /gene="ampD"
                     /locus_tag="VFX94_09155"
     CDS             complement(1427..1942)
                     /gene="ampD"
                     /locus_tag="VFX94_09155"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEX5768388.1"
                     /translation="MKIVESPNCDERPPNTEITLVILHSISLPPGNYGGDGIERLFTN
                     RLDPRAHPYFAEIAHLKVSSHYLIRRDGERVQFVPAEKRAWHAGLSSWRGRGRCNDFS
                     VGIELEGTEEDVFTAPQYRALAGLLRALQLRFPLRDIAAHSDVAPGRKTDPGARFDWT
                     RLLAALARARA"
     gene            complement(1929..>3045)
                     /locus_tag="VFX94_09160"
     CDS             complement(1929..>3045)
                     /locus_tag="VFX94_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5768389.1"
                     /translation="ENAVAALKAGAFDYVSKPVGLEQLRALVKSALSLPDSAEPAASG
                     AGRQLLGESAPLAHVRGLIAKLARTQAPVHISGESGSGKELAARLIHENGARREKPFV
                     PVNCGAIPETLVESELFGYRKGAFTGATEDRDGVFQAAHGGTLFLDEIAELPLHTQVL
                     LLRAIQERRVRKVGSTQEEPVDVRIICATNQSLAGMVEAGRFRRDLFYRLNVVDLTMP
                     PLRECREDIPLIAAHILQRLAAQNGVAPPRLSAGALQELMAYDFPGNVRELENILERA
                     LALSPGEELGSEALALHRIAQEDEAEESDKQAHSSRSELIEEPLPTYLDRLEREAILQ
                     ALGKTGFNRTAAARLLGITFRQLRYRMQRLNIRDENR"
BASE COUNT          478 a         1032 c         1024 g          511 t
ORIGIN      
        1 tcagatagca gctcgaaagc tgcgagcgca gcgtgccgga gttgaagagc gtcggcgtcg
       61 agctcatgaa gtcgaacgag gaaagaacgt cgtagaactc gatcgccctc gcttcccggt
      121 ccttctccct cagcgcgagc cccatggcga cccgcatgaa gaacgcctgc ggcagctcga
      181 tcctgcgccc gccaacgtgg agaaaatagc ggtcgtagag cgtctgcagg ccgagatagc
      241 cgaaatgcag gtcgcgcccg gccttcaacg ccctgccaag acgctccagg tcgaagcggg
      301 caagctcggg gtcgagcagc tccgccgcga tgccgcgctc gatgaagccc gggaagtagc
      361 ccgcgtagcg ggtgtccatg tccgcctggc ttgcctcctc gccgaggact tccaggcgga
      421 tcgtgtcgag cagcagccgc gcggtgacat agctgtagga ggggtctttc tcgatgagcg
      481 cgcgcgccgc gagcaccgcc gacttgcgca cctcctgcat cggcacgccg tcgtacaggt
      541 cgcgcagcgt cgcctcgagg attgccggcg ccgaggcgtc ggcgaggccc tcgcaggccg
      601 actccaccag acggcgcagg cgaacgcggt cgagcggctt gcgctcgctg ccgtccacca
      661 cgtggatctc cgaagcgccc ttggcggcgg ttttctcctg cgcgcgcgcc ttggcccacg
      721 cctcccggta cagcacgtac gcccgggcca cgtcgtgctc gcccgagcgc atcagcgcga
      781 gctcgacctg gtcctggatg tcctcaatct ggaatgttcc gccttgcggc tggcggcgca
      841 gcagggcggc caccacgttg tcggtcagcg cggccacgag ctcgcggatg cgcgccgaag
      901 ccgcgccctg cgatccgttc acggcgagga aagccttggt catcgcgacc gagatcttgg
      961 ccggctcgaa cgcgaccacc gcgccgttgc gccggatcac tttgtagtcg gcatacgcgc
     1021 cgcgcgccga ggtgccgcgc tcttcaaccc ccacgctcaa agacgaaagt gccgttgcgc
     1081 tgtcctgagc gacttgcatg ccgcctccca tccggtgttt gctctgtccc gggccgtcag
     1141 ccgtgacgcc ccccgcccct gccgtctgcc tactactttt agtgtccgac aaccgttctc
     1201 tgcctatatc tagtgattcc tcaccagaga ggtggagcct acaccagagt cttttttcct
     1261 ggcaagcgtg acgtgcgctt gttgcggcgc aatgctgcgc aatatgtcgc gtttgtagag
     1321 agacttcgat actagatcta gtagtgataa cgcgaggcac tacgcccgga ggtgcacgcg
     1381 ggcagaacag ctacatgtag tggctagccg cgggcaaaaa taaaattcag gcgcgtgcgc
     1441 gcgccagcgc ggcgagcagc cgcgtccagt cgaagcgcgc gcccgggtcg gtcttgcggc
     1501 cgggcgccac atcgctgtgc gcggcgatgt cgcgcagcgg aaatcgcaac tgcagcgcgc
     1561 gcagcaggcc cgcgagcgcg cggtactgcg gcgcggtgaa aacgtcctcc tcggtgcctt
     1621 cgagctcgat gccgacggag aagtcgttgc agcggccgcg gccgcgccag gacgagaggc
     1681 ccgcatgcca ggcgcgcttc tcggccggca cgaactgcac ccgctcgccg tcgcgccgga
     1741 tcaggtagtg gctggagacc ttgaggtggg cgatttcggc gaaataggga tgcgcccgcg
     1801 ggtcgagccg gttggtaaag agccgctcga tgccgtcgcc gccgtagttg cccggcggca
     1861 ggctgatcga gtgcaggatg accagggtga tttcggtatt cggcggccgc tcatcgcagt
     1921 tcggcgactc aacgattttc atctctgata ttcagtcgct gcatccggta gcgcagctgc
     1981 ctgaaggtga tgcccaggag gcgcgccgcc gcggtacggt tgaagccggt cttgccgagc
     2041 gcctgcagga tcgcctcgcg ctcgaggcgg tcgaggtagg ttggcagcgg ctcctcgatg
     2101 agctcggatc tggacgagtg cgcctgcttg tccgattcct ccgcctcgtc ctcctgggca
     2161 atccgatgca gggcgagcgc ctcggatccg agctcctctc cgggcgagag cgcgagcgcg
     2221 cgctcgagga tgttttccag ctcgcgcacg ttgccgggaa agtcgtacgc catgagctcc
     2281 tgcagcgcgc ccgccgagag ccgcggcggc gcaactccgt tctgggccgc gaggcgctgc
     2341 aggatgtgcg cggcaatgag cgggatgtcc tcgcgacatt cgcgcagcgg cggcatggtc
     2401 aggtccacca cattgagccg gtagaacaga tcgcgccgaa agcggccggc ttctaccatt
     2461 ccggcgaggc tctggttggt ggcgcagatg atgcgcacgt cgaccggctc ctcctgggtc
     2521 gagcccacct tgcgcacgcg ccgctcctgg atcgcgcgca gcagcagcac ctgggtatgc
     2581 agcggcagct cggcgatctc atcgaggaac agtgtccccc cgtgcgcggc ctggaaaacg
     2641 ccgtcgcgat cctcggtcgc cccggtgaag gcgcccttgc ggtagccgaa gagctcgctt
     2701 tccaccagcg tctcgggaat cgcgccgcag ttcacgggaa cgaagggctt ctcgcgacgc
     2761 gcgccgtttt cgtggatcag gcgcgcggcg agctccttgc cgctgcccga ttcccctgaa
     2821 atgtgcaccg gcgcctgcgt gcgcgccagc ttggcgatga ggccgcgcac atgggcgagc
     2881 ggcgcgcttt cgccgagaag ctggcggccg gctccgctcg ccgccggctc ggcgctgtcg
     2941 ggcagcgaaa gcgcggactt caccagcgcg cgcagctgct cgaggcccac cggcttggac
     3001 acgtagtcga acgccccggc cttgagcgcg gccaccgcgt tctcc
//
LOCUS       DASUNT010000453         4396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_852549, whole genome shotgun sequence.
ACCESSION   DASUNT010000453 DASUNT010000000
VERSION     DASUNT010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852549"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFX94_09165"
     CDS             complement(<1..267)
                     /locus_tag="VFX94_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5768390.1"
                     /translation="MIDLYTWPTPNGHKIHIMLEETGTPYRVIPVDIGAGDQFKAEFL
                     EISPNNKMPAMVDHDGPEGKGKPFALAESGAMLLYLASKTGKFLP"
     gene            complement(268..672)
                     /locus_tag="VFX94_09170"
     CDS             complement(268..672)
                     /locus_tag="VFX94_09170"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX5768391.1"
                     /translation="MASSWSSSPDYAGFWQRAVALLIDALIVVVTLAPLVVVLGPGGW
                     DLLGFLAAALAVIGFWGYCGATPGKIAVGLKIVDAASGGAPPLWRLVLRFFAYWVSAL
                     PLYLGFLWAAVDRRKQGWHDKIARTVVINSED"
     gene            complement(644..1624)
                     /locus_tag="VFX94_09175"
     CDS             complement(644..1624)
                     /locus_tag="VFX94_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX5768392.1"
                     /translation="MENQRIVLASRPKGWVTPDNFRLEKAEAPAPADGEVLVRNLWLS
                     LDPYMRGRISEAKSYVKGVEIGEVMVGQTVGEVLESKHPALKRGDHVLTQTGWQLYGT
                     TKEATKVDGTRAPLSYYLGVLGMPGMTAYFGLKDIGQPKAGETVVVSAASGAVGSVVG
                     QLAKLWGCRAIGIAGGREKCDYVTRELGFDACIDYKSANLREELKALGVDVYFDNVGG
                     EMLDILLARMKLFGRIVVCGMIADYNALEPYRVKNLRALLVNRLKVQGMIVFDSKERY
                     GEALKALGGYYAAGKLKTRESVVEGLANAPQGLVSLLKGGNFGKQLVKLA"
     gene            complement(1635..2219)
                     /locus_tag="VFX94_09180"
     CDS             complement(1635..2219)
                     /locus_tag="VFX94_09180"
                     /inference="COORDINATES: protein motif:HMM:NF028494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768393.1"
                     /translation="MPRSSSWRQAALVAVLLAACAHEPAPRKEAPSAAPVPLPALPAT
                     FMDEPDCPGCLGATITLRPDGSFLLREELGASEFYDFGRWRYDAGTLVLAGDRDTRNY
                     PLKDLRQAPQVEPLRGPFRMVGSYDGASFTECRTGLAWKLAQTRAAQSLSEEYAKQGK
                     ERVLVALDAQLEGSPETLRVFRPATVLDAPGCPG"
     gene            complement(2206..3192)
                     /locus_tag="VFX94_09185"
     CDS             complement(2206..3192)
                     /locus_tag="VFX94_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5768394.1"
                     /translation="MNFDYNEEQQLLADSVRRYLQKDYDFEARKKILASAGGWSEAVW
                     STFAEMGLTGLPLSPDYGGFGGGAVDLMSVMEAFGEALVVEPYVPTILGALAIAKGGD
                     EKLKREILPQVAQGTMRLALATGPRGVIGAPMADRIVFGSEGRLRIVAREAIEVRAYR
                     TVDDLAAGDVRLDGRGEPLAGGVLDEVLDFGAALSCAEAVGAMKFACDTTLEYLKTRR
                     QFGVPIGTFQALQHRMVDMVISYEQAKSMACLACSKLDQAESPRERARSVSAARIRIA
                     DAARHISQESVQLHGGMGMSEELKVSHTFRRLTALGRRFGDADQHLARFAAL"
     gene            complement(3189..4313)
                     /locus_tag="VFX94_09190"
     CDS             complement(3189..4313)
                     /locus_tag="VFX94_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5768395.1"
                     /translation="MDLNYSPEELAFRDEARSWLEANLPQDLRRKMADYAHLSKDDLV
                     RWHKILAKQGWVAPAWPKEWGGTGWNVVQRYIFEEELGYVGSPPLIPFGLTMCAPVLL
                     QFGTEAQKKRFLPRIYNGDDFWCQGYSEPGSGSDLASLKTKAVRDGDHYVVNGQKTWT
                     SYAHYADWIFCLVRTDPTVEKRQEGISFLLMDMRSPGITVRPLILMDGAHAVNEVFFD
                     DVKVPVENLVHKENQGWTVAKYLLGYERMNTGRIGESKRELRKLKENFSSKLSDVRFR
                     DRLTRVEVELMALEITNLRFLDRMRRTGQPPGADVSMLKIKGTEIQQALTELAMQAAD
                     PNARETQPLERRYLDIRKTTIYAGSNEIQRNIIAKMTLGL"
BASE COUNT          714 a         1488 c         1419 g          775 t
ORIGIN      
        1 aggcaggaac ttccccgtct tggatgcgag gtacagcagc atcgcgcccg actcggcgag
       61 cgcgaagggc ttgcctttgc cttccggtcc gtcgtggtcg accatcgccg gcatcttgtt
      121 gttcggcgag atctcgagga actcggcctt gaactggtcg cccgccccga tgtcgaccgg
      181 gatgacgcgg tacggcgtgc cggtctcctc cagcatgatg tggatcttgt ggccgttggg
      241 tgtcggccac gtatagaggt ctatcaatca atcctccgag ttgatgacga cagtgcgcgc
      301 gatcttatcg tgccagccct gcttgcgccg gtcgaccgcc gcccagagga agccgaggta
      361 gagcggcagc gcggaaaccc aataggcgaa gaagcgaagc acgaggcgcc agagcggcgg
      421 cgcgccgccg ctcgcggcat cgacgatttt cagcccgacg gcgatcttcc cgggcgtcgc
      481 gccgcaatag ccccagaagc cgattacggc gagggccgcg gcgaggaaac ccaacaagtc
      541 ccagccgccc gggcccagca ccaccacgag cggcgcgaga gtcaccacga cgatcagcgc
      601 gtcgatgagc aaggccaccg cgcgctgcca gaacccggcg tagtcaggcg agcttgacca
      661 gctgcttgcc aaaatttccg cccttgagga gcgagacgag tccctgcggc gcgttcgcca
      721 ggccctcgac cacggattcg cgcgtcttga gcttgcccgc agcgtagtag ccgccgagcg
      781 ccttcagcgc ctcgccgtag cgctccttcg agtcgaacac gatcatgccc tggaccttga
      841 gccggttgac gagcagcgcg cgcaggttct tcacgcggta aggctcgagc gcgttgtagt
      901 cggcaatcat gccgcacact acgatgcggc cgaagagctt catgcgcgcg agcaggatgt
      961 cgagcatctc gccgccgacg ttgtcgaagt agacgtcgac gccgagagcc ttcagctcct
     1021 cgcgcagatt tgccgacttg tagtcgatgc aggcgtcgaa gccgagctcg cgcgtcacgt
     1081 agtcgcactt ctctcgcccg ccggcgatgc cgatcgcgcg gcagccccac agcttcgcga
     1141 gctggccgac gacgcttccc accgcgcccg aagcagccga tacgaccacg gtctcgccgg
     1201 ccttcggctg cccgatgtcc ttcaggccga aatacgctgt catccccggc atgccaagca
     1261 cgccgaggta gtagctcagc ggtgcgcgcg tgccatcgac tttggtcgcc tcctttgtcg
     1321 ttccgtacaa ctgccagccc gtctgggtga gcacatgatc gccgcgcttg agcgcaggat
     1381 gctttgattc gagcacctcc ccgaccgtct ggccgaccat gacctcgccg atctctacgc
     1441 ccttcacgta gcttttcgcc tcgctgatgc gcccacgcat gtacggatcg agcgaaagcc
     1501 agaggttcct gaccagcacc tctccatccg cgggcgccgg agcttctgct ttctccagtc
     1561 gaaaattgtc gggcgtcacc catcccttgg ggcggctggc gaggacgatg cgctggtttt
     1621 ccatgcgccg aagtctagcc cgggcagccg ggcgcatcga gcactgtcgc ggggcgaaac
     1681 acgcgcagcg tctccggcga gccttcgagc tgcgcgtcga gcgcgaccag gactctttct
     1741 ttcccttgct tcgcgtattc ctcgctcagg ctctgcgcag cgcgcgtctg ggcgagcttc
     1801 caggcgaggc cagtgcggca ttccgtgaag ctcgcgccgt cgtagctccc caccatgcga
     1861 aacgggccgc gcagcggctc cacttgcggg gcttgcctga ggtccttcag cgggtagttg
     1921 cgggtatccc gatcgcccgc aagcaccagg gtgcccgcgt cgtaacgcca gcgcccgaag
     1981 tcgtagaact ccgaggcgcc gagctcctcg cgcagcagga aagatccgtc ggggcgtagc
     2041 gtaatggtgg cgccgaggca gcccgggcag tcgggctcgt ccatgaacgt ggcaggcagc
     2101 gcggggagcg gcaccggtgc tgcggacggg gcttccttcc tcggggcggg ctcgtgcgcg
     2161 caggcggcaa gcaacacggc cacgagcgcc gcctggcgcc agctgctaga gcgcggcaaa
     2221 gcgcgcgaga tgctggtccg cgtctccgaa gcgccggccg agcgcggtga ggcgtctgaa
     2281 cgtatggctt accttgagct cctcgctcat gcccatgccg ccgtgcagct gcaccgactc
     2341 ctggctgatg tggcgcgcgg cgtcggcgat cctgattctt gccgccgaca ccgaacgcgc
     2401 ccgctcgcgt ggactctcgg cctgatccag cttcgagcag gcgaggcagg ccatcgactt
     2461 cgcctgctcg tacgagatca ccatgtcgac catgcggtgc tgcagcgcct ggaaggtgcc
     2521 gatcggcacg ccgaactgcc ggcgcgtctt gaggtattcg agcgtggtgt cgcacgcgaa
     2581 cttcatcgcc ccgaccgcct cggcgcagga aagcgcggcg ccgaagtcga gcacctcgtc
     2641 aagcacgccg ccggcgagcg gctcgcccct gccgtcgagg cgaacgtcgc ccgccgcgag
     2701 atcgtccacg gtgcggtagg cgcgcacctc gatcgcttcg cgcgccacga tgcgcagcct
     2761 cccctccgat ccgaagacga tccggtcggc catgggcgcg ccgatgacgc cgcgcggccc
     2821 cgtggcgagc gcaagccgca tcgtgccctg cgcgacctgc ggcaggattt ctcgcttcag
     2881 cttctcgtcg ccgcccttgg cgatcgcgag cgcgccgagg atggtgggaa cgtacggctc
     2941 gaccacgagc gcctcgccga aggcctccat cacgctcatg aggtcgacag ccccgccgcc
     3001 gaagccgccg tagtccggcg agagcggcag gccggttaga cccatttccg cgaacgtcga
     3061 ccacacagcc tcgctccagc cgccggcgga ggcgagaatc ttcttcctcg cctcgaaatc
     3121 gtagtccttc tgcaggtagc ggcgaaccga gtctgcgaga agctgctgct cttcgttgta
     3181 atcgaagttc atagacccaa cgtcatcttg gcgatgatgt tcctctggat ctcgttggag
     3241 cccgcgtaga tcgtggtctt gcgtatgtcg aggtagcggc gctcgagcgg ttgcgtctcg
     3301 cgggcgttcg ggtcggccgc ctgcatggcg agctcggtga gcgcctgctg aatctcggtg
     3361 cccttgatct tgagcatcga tacgtcggcg cccggcggct ggccggtgcg gcgcatgcgg
     3421 tccaggaagc gcaggttggt gatctcgagt gccatcagct cgacctccac gcgcgtcagg
     3481 cgatcgcgaa agcggacgtc acttaatttt gaggaaaaat tctctttcag cttccgcagc
     3541 tcccgtttcg attcgccgat ccggccggta ttcatgcgct cgtagccgag caggtacttc
     3601 gccaccgtcc agccctggtt ttccttgtgc acgaggtttt cgaccgggac tttcacgtcg
     3661 tcgaagaaga cctcgttcac cgcgtgagca ccgtccatca ggatgagagg gcgcacggtg
     3721 attccgggcg agcgcatatc catgagcagg aacgagatcc cttcctggcg cttctcgacc
     3781 gtgggatcgg tgcgcaccag gcagaagatc cagtcggcgt agtgcgcgta ggacgtccag
     3841 gtcttctggc cgttgacgac gtagtggtcg ccgtcgcgga ccgccttcgt cttcagcgag
     3901 gcgaggtcgc ttcccgagcc cggctccgag tacccctggc accagaagtc gtcgccattg
     3961 tagatgcgcg gcaggaagcg cttcttctgc gcctcggtgc cgaactgcag cagcaccggc
     4021 gcgcacatcg tcaggccgaa gggaatgagc ggcgggctgc cgacgtagcc gagctcctcc
     4081 tcgaagatgt agcgctgcac gacgttccag ccggtgccgc cccactcctt cggccaggcc
     4141 ggcgcgaccc agccctgctt ggcgaggatc ttgtgccagc gcaccaggtc gtccttcgac
     4201 aggtgcgcgt agtcggccat cttccggcgc aggtcctgcg gcaggttcgc ttccagccag
     4261 gagcgcgcct catcgcggaa cgccagctct tcgggggaat agttcagatc cattagagaa
     4321 tggtaactcc gccgtcggcg acgatgcact gtccggtgat gaaggccgca gcgtcggagg
     4381 cgaggaatac cgcgat
//
LOCUS       DASUNT010000454         5340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_764813, whole genome shotgun sequence.
ACCESSION   DASUNT010000454 DASUNT010000000
VERSION     DASUNT010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764813"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2588
                     /locus_tag="VFX94_09195"
     CDS             <1..2588
                     /locus_tag="VFX94_09195"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5768396.1"
                     /translation="KVFPLPVDANSGSGLAVVERRVVHNPDSEADGVPEAARRGNRAR
                     GNRASLYAPMMSRDKAIGVIWVAREQPGPFSETEIALLKTFADQAVIAIENARLFNET
                     KDALEQQTATAEILKVISGSPTDVQPVFDAIARSALRIFGGRDVGVGFVEGDAIQIRA
                     GTLSALGKATPPRIPLNRDTYAGRAVLDGTEFNVADTEAPGTPPLTRERARLAGWRAI
                     ALVPMLREGVPIGHISVHRAEPVAFDDKQFALLRTFAAQAVIAIENVRLFNETKEALE
                     RQTATAEILKVMSASLTDVQPVFDAVALNAARVCGVDDVAILQTEGANLRRVSHYGNV
                     PVSPDSLVPVPPGSVNERVLSDHRTLHIPDTLAPSFTREFPGSQFPAMGVRALLATPL
                     VRDGVAVGVIHLRRSEARPFTDKQIGMLETFADQAVIAIENVRLFKELQERTEALTRS
                     VSQLTALGEVGQAIGSTLELDEVLRTIVQRAVELAGMDAGSIYEYNAREERFYLRAAQ
                     NVAEDILRVYRDTPLRLGEGSIGRAGSTREPVMIEDVLDDSYRSRARQALIRSGVRAL
                     LTVPLLREDLLLGAIVVSRKTPGRFEREVIELLKTFATQSAVAIQNARLFRELAEKSR
                     ELEEASRHKSQFLASMSHELRTPLNAILGFNEMILGQVYGEVPPDMKEPLEDIQKSGK
                     HLLRLINNVLDLAKIEAGKMELALADYSVQDTVESVRSTLRPLAEAKGLELVVRVPED
                     LPLAYGDGGRLTQCLMNLAGNSLKFTKEGRVEIAVEAENGHLRWRVSDTGIGIPPDKI
                     PTLFSEFKQTDATIASEYGGTGLGLSITKKFVEMHGGRIWVESEPGKGSVFTLEVPLR
                     VTARA"
     gene            2585..2959
                     /locus_tag="VFX94_09200"
     CDS             2585..2959
                     /locus_tag="VFX94_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5768397.1"
                     /translation="MSAKTILYIEDNEMNRKIVRDLLKRTKYTLIEANDGEAGVAKAL
                     ETHPDLILMDIQLPKISGLEATKRLRADAATANTPIIAITSFALSGDDVRAKEAGATA
                     YLAKPYSPFDLLNLIRKILPET"
     gene            2956..>5340
                     /locus_tag="VFX94_09205"
     CDS             2956..>5340
                     /locus_tag="VFX94_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5768398.1"
                     /translation="MSERTRKRATRRSGKRKAGKRDTLQHRLDEALAREAASAEILAS
                     IRSSRESPQAALDAIVRNLQRLFGTAFAAVFLVRDGKIEIAAAAGKARFVQGMRAGGP
                     MRLADEELLAVRAMRRAQVLRLCPVIGNPDAPPKTERLARKTGFDSIIAAPMLRDGDA
                     IGAVGTARTEAVPFDDAQAALIQSFADQAVIAIENARLFNELKTTNSSLREALEQQTA
                     TSEILGVISSSPTAVQPVFKTIGENARLLCRADVSVISHFDGELLQLTELHLAGEGAE
                     AVQGVFPMRPDAATVTARTFRERAIVHVEDVLAEPGYANKELARIARWRGALGVPMMR
                     EGQVAGVIFVARERAGLFSESQVALLKTFADQAAIAIENVRLFNETKEALEHQKASTG
                     ILRVVSSSIADAAPVFEAIADSCAQIFQSEQIGINLVGEDGAIHLGAYRGRNGEQYIE
                     WMRTGPTREGGKVLNLRRAVAHFPDVEHGADVPEAIRQGCRITGSKSIIFAPMISGGR
                     GIGAVFVGRDRVGLFSEKEIALLTTFCEQAVIAIQNARLFNQTRQSLERQTATAEILK
                     VIAASPSDTQPVFEAIARSAQQLFAAHSAAVARRIEDMLHLAAHTAFSEAGAESLRKL
                     FPTEITGQGAFGKAVLTRKPAWIADVETDPTYSEEFRRGARERGSRSLLAVPMLREGA
                     AIGVIGVTRREPGTFSDHQVQLLQTFADQAVIAIENVRLFNETKEALEKQTATSEILG
                     VISGTPTDTQPVFEAIVRRATKLCEAVSANVFRYDGEQLHWVASVGWPPDILSAA"
BASE COUNT          963 a         1806 c         1771 g          800 t
ORIGIN      
        1 agaaggtgtt ccccctgcca gtggacgcca attccggctc ggggctcgcc gtcgtcgagc
       61 gccgggtcgt ccataacccc gactcggaag ccgacggcgt gcccgaggcc gcgcgccgcg
      121 gaaaccgcgc ccggggaaac cgggcgtcgc tctatgcgcc gatgatgtcg cgcgacaagg
      181 cgatcggcgt gatctgggtg gcacgcgagc agcccggtcc gttcagcgaa acggaaatcg
      241 cgcttctcaa gactttcgcc gatcaggccg tgatcgcaat cgagaacgcc cgcctgttca
      301 acgaaacgaa agacgcgctc gagcagcaga ccgcgaccgc cgagatcctg aaggtcatca
      361 gcggatctcc caccgacgtg cagccggtct tcgacgcaat cgcccggagc gcgctgcgga
      421 ttttcggcgg tagggacgtg ggtgtcgggt tcgtggaggg cgatgcgatc cagatccgcg
      481 cggggacgct tagcgccctc ggcaaggcta ctccgccacg catcccgctc aaccgggaca
      541 cctacgccgg gcgcgcagtg ctcgatggaa cggagttcaa tgtcgccgac accgaagcgc
      601 ccggcacgcc gccgctcact cgcgagcgcg cccgcctcgc cggctggcgc gcgatcgcgc
      661 tggttccgat gctgcgcgaa ggagtgccga tcggccacat ttccgtccac cgcgccgagc
      721 ccgtggcgtt cgacgacaag cagttcgcgc tgctcagaac cttcgccgcg caggcagtga
      781 tcgcgatcga gaacgtccgc ctgttcaacg agaccaagga ggcgctcgag cgccagacgg
      841 ccaccgcgga gatcctgaag gtgatgagcg cctcgctcac ggatgtgcag ccagtgtttg
      901 acgcggtcgc cctgaacgcc gctcgcgtgt gcggcgtaga cgatgtggcc attctgcaaa
      961 ccgagggtgc gaacctgcgg cgcgtctcgc actacggcaa cgtgccggtg tccccggaca
     1021 gcctcgtgcc cgtgccaccc gggagcgtca acgagcgcgt tctgtccgac caccgcaccc
     1081 tgcatatccc cgatacgctg gcgccgtcct tcacacggga gtttccgggc tcgcaattcc
     1141 cggccatggg tgttcgcgcg cttcttgcga caccgctggt tcgcgacggc gtcgcggtcg
     1201 gcgtgatcca cctgcggcgc tcggaggcgc ggccgttcac cgacaagcag atcgggatgc
     1261 tcgagacctt cgccgaccag gcggtgatcg cgatcgagaa cgtgcgcctg ttcaaggagc
     1321 ttcaggagcg caccgaggcg ctcaccaggt cagtaagcca gctcacggcg cttggcgagg
     1381 tcggccaggc gatcggctcg accctcgagc tcgacgaggt gctgcgcact atcgtgcagc
     1441 gcgcggtgga gcttgccggc atggacgccg gctcgatcta cgagtacaac gcccgcgagg
     1501 agcgcttcta cctgcgcgcg gcgcagaacg tcgccgagga catcctgcgg gtgtaccgcg
     1561 acacgccgtt acggctgggc gaaggctcca tcgggcgcgc cggaagtacg cgcgagccgg
     1621 tgatgatcga agacgtgctc gacgacagct accgcagccg cgcacgccag gcgctcatca
     1681 ggtcgggggt ccgggcgctg ctcacggtgc cgctgctgcg cgaggacctg ctgctcggcg
     1741 cgatcgtggt gagccgcaag acgcccgggc ggttcgagcg cgaggtgatc gagctcctca
     1801 agaccttcgc cacgcagtcg gcggtggcga tccagaacgc gcgcctgttc cgcgagctcg
     1861 ccgagaagag ccgcgagctc gaggaggcga gccggcacaa gtcgcagttc ctcgccagca
     1921 tgagccacga gctgcgcacg ccgctcaatg ccatcctcgg cttcaacgag atgatcctcg
     1981 gacaggtcta cggcgaggtg ccgcccgaca tgaaggagcc gctcgaagac atccagaaaa
     2041 gcggcaagca cctgctgcgg ctcatcaaca acgtgctcga tctcgccaag atcgaggcgg
     2101 gaaagatgga gctcgcgctc gccgactact cggtgcagga cacggtggag agcgtgcgct
     2161 ccacgctgcg cccgctggcg gaggcgaaag gactcgagct cgtcgtgcgc gtgcccgagg
     2221 acctgccgct cgcctacggc gacggcggcc ggctcacgca gtgcctcatg aacctcgccg
     2281 gcaactcgct caagttcacc aaggagggcc gagtggagat cgcggtcgag gccgagaacg
     2341 gccacctgcg ctggcgcgtg tccgacaccg gcatcgggat cccgcccgac aagatcccga
     2401 cgctcttctc cgagttcaag cagaccgacg ccaccatcgc gagcgagtac ggcggcacgg
     2461 ggctcgggct ttcgatcacc aagaagttcg tcgagatgca tggcggccgc atctgggtcg
     2521 agagcgagcc cggcaagggt tcggtcttca ccttggaggt cccgctcagg gtgacggcgc
     2581 gggcatgagc gcgaagacca tcctttacat cgaagacaac gagatgaacc gcaagatcgt
     2641 gcgcgatctg ctgaagcgca cgaagtacac gctcatcgaa gcgaatgacg gcgaggcagg
     2701 ggtggccaag gcgctggaaa cacaccccga cctgattctc atggacattc agctccccaa
     2761 gatctcgggg ctcgaggcga cgaaacggct gcgtgccgac gccgccaccg cgaacacgcc
     2821 catcatcgcg atcacttcct tcgcgctttc cggcgacgac gtcagagcaa aggaagcggg
     2881 ggccaccgct tacctcgcca agccctacag cccgttcgac ctcctcaacc tcatccgcaa
     2941 gatccttccc gagacatgag cgagcgcacc cgcaagcgcg ccacaagaag aagcggcaag
     3001 cgcaaggcgg gcaagcgcga cacgcttcag cacaggctgg atgaggccct cgcgcgcgag
     3061 gccgcgagcg ccgagatcct cgcctccatc cgcagctcac gggagagtcc gcaggccgcc
     3121 ctggatgcca tcgtgcgcaa cctgcagcgg cttttcggaa ccgcgttcgc ggccgtgttc
     3181 ctcgtccgcg acggcaagat cgagatcgcc gcggcagccg gcaaagcgcg cttcgtccag
     3241 ggcatgcgcg cgggcggtcc catgcggctc gcggacgagg agctgctcgc ggtgcgcgcc
     3301 atgcgccgcg cgcaggttct gcgcttgtgt ccggtcatcg gcaacccgga tgcgccgccg
     3361 aagacagagc gcctggcgag gaagaccggg ttcgattcca tcatcgccgc gccaatgctg
     3421 cgcgacggcg acgcgatcgg cgccgtcggt accgcgagga ccgaggcggt tccgttcgac
     3481 gacgcgcaag ccgccctcat ccagtctttc gccgaccagg cggtgatcgc gatcgagaac
     3541 gcgcgcctgt tcaacgagtt gaaaacgacg aacagcagcc tgcgcgaggc gctcgagcag
     3601 cagactgcga ccagcgagat ccttggggtg atcagcagct cgccgaccgc cgtgcagccg
     3661 gtgtttaaga ccatcggcga aaacgcgcgg ctcttgtgcc gggccgacgt cagcgtcatt
     3721 tcgcatttcg acggcgagct gctgcagctc acggagcttc accttgccgg ggaaggggct
     3781 gaggcggtgc agggcgtgtt tcccatgcgc cccgacgccg ctacggtcac tgcgcgcact
     3841 ttccgcgagc gcgccatcgt ccacgtcgaa gacgttctcg ccgaaccggg ctacgccaac
     3901 aaggaacttg cgcgcatcgc gcgctggcgc ggtgcgctgg gagtcccgat gatgcgggaa
     3961 gggcaggtgg ccggcgtgat cttcgtcgcg cgggagcgag cgggactatt ctccgaatcg
     4021 caggtcgcgc tgctcaaaac cttcgccgac caggcggcaa tcgcgatcga gaacgtgcgc
     4081 ctgttcaacg agaccaagga ggcgctcgag caccagaagg cctcgaccgg gatcctgcgc
     4141 gtggtgagca gctcgatcgc cgacgcggct ccggtgttcg aggcgatcgc cgacagttgc
     4201 gcccagatct tccagtccga gcagatcggc attaacctgg tcggcgagga tggcgcgatc
     4261 cacctcgggg cctatagggg gcgcaacggc gagcagtaca tcgagtggat gcgcaccggt
     4321 ccgacgcgtg agggcgggaa agtgctcaac ctgcggcgcg cagtggctca ctttcccgac
     4381 gtcgagcatg gcgcggacgt tccggaggcg attcgccagg gctgccggat caccggctcg
     4441 aagtcgatta tcttcgcgcc catgatctcc ggcggccgcg ggatcggcgc ggtgttcgtc
     4501 ggacgcgacc gggtcgggct cttctcggag aaagaaatcg cgctgctcac caccttctgc
     4561 gagcaggcgg tgatcgcgat ccagaacgcg cggctcttca accagaccag gcaatccctc
     4621 gaacggcaga ccgccaccgc cgaaatcctc aaagtcatcg ccgcttcgcc ctccgacacg
     4681 cagccggtgt tcgaggccat cgcgagaagt gcgcagcagc ttttcgccgc ccactccgcc
     4741 gcggtcgcgc ggcgaatcga ggacatgctc cacctcgccg cgcacaccgc attcagcgag
     4801 gccggagccg aatcgctcag gaagcttttc cccacagaga tcacgggcca aggtgcgttc
     4861 ggcaaggccg tgctcacccg caagccggcc tggatcgccg acgtggaaac cgacccgacc
     4921 tactccgaag aattccgcag aggcgcgcgc gagcgcggct ctcgcagcct gctcgctgtg
     4981 ccgatgttgc gcgagggtgc tgccatcggc gtcatcggcg tcacgcgccg cgagccggga
     5041 acattcagcg accaccaagt tcagctcttg cagaccttcg ccgaccaggc ggtgatcgcg
     5101 atcgagaacg tgcgcctgtt caacgagacc aaggaggcgc tcgagaagca gaccgcgacg
     5161 tccgagattc tgggcgtgat ctccggcacg ccgaccgaca cacagccggt gttcgaggct
     5221 atcgtgcgca gagcgaccaa gctgtgcgaa gcagtgtctg cgaacgtttt ccgctatgac
     5281 ggcgagcagc tgcattgggt agcctcggtc ggctggcctc ccgatattct ttcagcggcc
//
LOCUS       DASUNT010000455         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_765091, whole genome shotgun sequence.
ACCESSION   DASUNT010000455 DASUNT010000000
VERSION     DASUNT010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765091"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VFX94_09210"
     CDS             complement(<1..878)
                     /locus_tag="VFX94_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEX5768399.1"
                     /translation="MSEASPAALVPYVTGGAFALAFIFGAVGNKTDFCTMGAVSDWVN
                     MGETSRMRMWLLAMAVALLGATALEVAGLVDLSKSIYPGPNFTWLSYLVGGFLFGFGM
                     TLGSGCGSKTLIRVGTGNLKSLVVYVFLGIAAYMTLRGLFGAFRVGVLEKATIVLPQG
                     QDLPSLLGVSPAPLAALIGGGMIAFVYASKAFRSSFHYTLGGVVTGLVVVGGWYVSGV
                     IGHVDEDPNTLQEAFVATNTGRMESFSFVAPLAYTLEYLMLWTDKSKIVTYGIASAAG
                     VIAGSAAYALATRTFR"
     gene            complement(891..1118)
                     /locus_tag="VFX94_09215"
     CDS             complement(891..1118)
                     /locus_tag="VFX94_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX5768400.1"
                     /translation="MNFDRELDARGLNCPLPELRAKKALNEMSSGQVPKIVATDPGSV
                     KDFQAFSKQTGNELLAHDVSQKDFTFFLRRK"
     gene            1214..2107
                     /locus_tag="VFX94_09220"
     CDS             1214..2107
                     /locus_tag="VFX94_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX5768401.1"
                     /translation="MKYKDYYKVLGVERGASEEDIKKAYRKLARKYHPDVSKDTNAKE
                     RFQEVSEAYETLRDKEKRAAYDSLGSFRPGQDFRPPPDWFERFGAGRTEDLHGVDLGE
                     LFEQMGLFGRAGRRRSRSVFPGEDYEVPVRLTLEEAFRGTERTVQVDGRSFTARIPRG
                     ATDGQRLRLRGKGGPGMNGGPPGDLYLQIALEPHPLYRVSAHDLEIEVPLAPWEAALG
                     AEVDVPTLEGRVTMKVPPGSRPGQKLRLGGKGLPKPGGGAGDLYAVLTVAVPATLTER
                     EKALYEELRDNSRFNPRLRFG"
     gene            2141..2533
                     /locus_tag="VFX94_09225"
     CDS             2141..2533
                     /locus_tag="VFX94_09225"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEX5768402.1"
                     /translation="MALTEQEKQELRGDIERRRDTLITELHADAERVRGDRFADLAGP
                     APDPGDESVATLIADLGHADMARDLAELRALEAARTRLADGSYGTCAECGGDIGIERL
                     RANPAALRCVDCQRMHEKTFAGPNTSSL"
     gene            complement(2546..>2990)
                     /locus_tag="VFX94_09230"
     CDS             complement(2546..>2990)
                     /locus_tag="VFX94_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX5768403.1"
                     /translation="TRKLERVLGLHSESVIQDVDIPISRAAEFLDFYSREIGLWPQWI
                     CPIGSQSPSGRFVLYPVKSTWYVNFGFWDVKRTREAHPPGHFNRLIEDKVSELGGIKS
                     LYSDSFFTERDFHDRYGGTAYDALKKKYDPARVFPRLYDKCVLKA"
BASE COUNT          517 a          998 c         1035 g          440 t
ORIGIN      
        1 cagcggaagg tacgggtggc gagcgcgtag gcagccgagc cggcgatgac gccggcggcc
       61 gaggcgatgc cgtaggtaac gatcttcgat ttgtcggtcc acagcatcaa atactcgagc
      121 gtgtaggcga gcggcgcgac gaaggagaac gactccatgc gcccggtatt cgtggcgacg
      181 aatgcttcct gcagcgtgtt gggatcttcg tcgacgtggc cgatgacccc gctcacgtac
      241 cagccgccca cgaccacgag cccggtaacg acgccgccga gggtgtagtg gaagctcgag
      301 cgaaacgcct tgctcgcgta gacgaacgcg atcatgccgc cgccgatgag cgcggcgagc
      361 ggcgccgggc tcacgccgag caggctcggc aggtcctggc cctgcggcag cacgatcgtc
      421 gccttctcga gcacgccgac gcgaaaggcg ccgaacagtc cgcgcagcgt catgtaggcg
      481 gcgatgccga ggaacacgta caccacgagc gacttgaggt tccccgtgcc gacgcggatc
      541 agagtcttcg agccgcagcc cgagccgagc gtcatgccga agccgaacag aaatccgccg
      601 acgaggtagg agagccaggt gaagttgggg ccgggataga tcgacttcga caggtccacc
      661 agccccgcga cctcgagcgc ggtcgcgccg agcagcgcca ccgccatcgc gagcagccac
      721 atgcgcatgc ggctggtctc ccccatgttt acccagtcgg agaccgcgcc catggtgcag
      781 aagtcggtct tgttgccgac ggcaccgaaa atgaacgcga gcgcgaacgc cccgccggtg
      841 acgtagggga cgagcgcggc ggggctcgct tcgctcactg cgggaagcgc ctacttgcgc
      901 ctgaggaaga aggtaaagtc cttctgcgac acgtcgtgcg cgagcagctc gtttccggtc
      961 tgcttcgaga atgcctggaa gtccttcacc gacccggggt cggtggcgac gatcttgggc
     1021 acctgcccgg agctcatctc gttcagcgcc ttcttcgcgc gcagctccgg gagcgggcag
     1081 ttgagcccgc gggcgtcgag ttctcggtcg aagttcatgg ctgcgcctaa gtgtatatcc
     1141 tccacgacat ggagtttcgc gccgcatctg ccggcacatt gcgcgctgac cgggccgccc
     1201 ccatattggc gggatgaagt acaaggacta ctacaaggtc ctcggcgtcg agcgcggggc
     1261 ttccgaggag gacatcaaga aggcctaccg caagctcgcg cgcaaatacc accccgacgt
     1321 ctcgaaggac acgaacgcga aggagcggtt ccaggaggtg tcggaggctt acgagacgct
     1381 gcgcgacaag gaaaaacggg cggcctacga cagcctgggg agcttccgtc ccggccagga
     1441 tttccggccc ccgcccgact ggttcgagcg cttcggcgcc gggcgcacgg aggacctgca
     1501 cggcgtggac ctgggggagc tcttcgagca gatgggcctt ttcggccgcg ccgggcggcg
     1561 gcgctcgcgc agcgtctttc ccggcgagga ctacgaggtg ccggtccggc tcacgctgga
     1621 agaggccttc cggggaaccg agcgcacggt ccaggtcgac ggccgcagct tcaccgcccg
     1681 catcccgcgc ggcgcgaccg acgggcagcg gctgcggctg cgcggcaagg gcggcccggg
     1741 catgaacggc gggccgccgg gggacttgta cctccagatc gcgctcgagc cgcatccgct
     1801 gtaccgcgtg agcgcgcacg acctcgagat cgaggtgccc ctcgctccct gggaagccgc
     1861 gctcggcgcg gaagtcgacg tgccgaccct ggaaggccgc gtcacgatga aggtcccgcc
     1921 gggctcgcgc cccggacaga agcttcgcct gggcggcaag ggactgccca agcccggcgg
     1981 cggcgccggc gatctctacg ccgttctcac cgtcgccgtg cccgcgacgc tcaccgagcg
     2041 cgagaaggcg ctgtacgagg aattgcgcga caactcgcgc ttcaaccccc gcctgcgctt
     2101 cggctagagc gccggcacgg cgcttgcaac cggcggccgc atggcgctta cggaacagga
     2161 gaagcaggag ctgcgcggcg atatcgagcg ccggcgcgac acgctgatta cggagctgca
     2221 cgcggacgcg gagcgcgtgc gcggcgaccg cttcgcggat cttgccggcc cggcgccgga
     2281 cccgggcgac gaatcggtcg cgacgctcat cgccgacctc ggccacgccg acatggcgcg
     2341 cgatctggcg gagctgcgcg cgctcgaggc cgcgcgcacc cgtctcgccg atggcagcta
     2401 tggcacctgc gccgaatgcg gcggcgatat cggcatcgag cgcctgcgcg ccaacccggc
     2461 tgcgctgcgc tgcgtcgact gccagcgcat gcacgaaaag accttcgccg ggccgaacac
     2521 ctcgagcctt tagccgaatt ccgccttatg ctttgagcac gcatttgtcg tagagccgcg
     2581 ggaagacgcg cgccgggtcg tattttttct tgagggcgtc gtaggcggtg ccgccgtagc
     2641 ggtcgtggaa gtcgcgctcg gtgaagaagc tgtcggagta cagcgacttg atgccgccga
     2701 gctcgctcac cttgtcctcg atcaggcggt tgaagtgccc gggcgggtgc gcctcgcgcg
     2761 tacgcttcac gtcccagaag ccgaagttca cgtaccacgt gctcttcacc gggtagagca
     2821 cgaaccggcc ggagggggat tgcgacccga tcggacagat ccactgcggc cacaagccga
     2881 tctcgcgcga gtagaagtcg aggaattcgg ccgcgcgcga gatcgggata tcgacatcct
     2941 ggatcaccga ttccgaatgc agcccgagca cgcgctcgag ctttcgggtg
//
LOCUS       DASUNT010000456         2828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_852974, whole genome shotgun sequence.
ACCESSION   DASUNT010000456 DASUNT010000000
VERSION     DASUNT010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852974"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2251)
                     /locus_tag="VFX94_09235"
     CDS             complement(<1..2251)
                     /locus_tag="VFX94_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5768404.1"
                     /translation="MNAPLIGARVQRKEDYRFLTGSGQFTDDVALPNQTYAVFVRSPH
                     AHAKIRNISSETARTSSGVLAVYTGEDLAAAKVGGLPCGWLITDVNGKPMKEPPYPPL
                     AQGKVRHVGERVAVVVAQTPEQARDAAEKIQVDYEELPAVADVGKARNGPAIHELAPD
                     NTCYVWALGDKAAVEAAFAKAAHVTKLDFWNNRLVPNAIEPRSANAVYSRADDGYTLY
                     VASQNPHVERLLMTAFVLGLPESKVRVVAPDVGGGFGSKIYLYGEDVVVTWAAKQLNR
                     PVKWTAERSESYVSDAHGRDHLTTAELALDKDGKFLAMRVRTTANLGAYLSTFASCIP
                     TILYATLLAGQYTTPLIYCEVTAVFTNTAPVDAYRGAGRPEATYVVERLLMTAFVLGL
                     PESKVRVVSPDVGGGFGSKIYLYGEDVVVTWAAKQLNRPVKWTAERSESYVSDAHGRD
                     HLTSAELALDKDGKFLAMRVRTTANLGAYLSTFASCIPTILYATLLAGQYTTPHIYCE
                     VTAVFTNTTPVDAYRGAGRPEATYVVERLVETAAREMKMSPVEIRKQNFIREFPYQTP
                     VALCYDTGNYQATLDAALKLADVQNFSKRKEESAKHGKLRGIGYACYIEACGIAPSNV
                     AGSLGARAGLFEAGEIRVHPTGTVTVFTGSHSHGQGHETSFAQVVADRLGLPIEAVEV
                     VHGDTSKILFGMGTYGSRSIAVGGTAIVKALDKIVAKGKKIAAHLLEASEADIEYDKG
                     EFKVAGTDKK"
     gene            complement(2248..2727)
                     /locus_tag="VFX94_09240"
     CDS             complement(2248..2727)
                     /locus_tag="VFX94_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5768405.1"
                     /translation="MTINVSMKVNGKPVSAELEARTLLVTYIRENLRLTGTHVGCDTA
                     QCGACTVLMNGRAIKSCSIVAMQAEGADIMTIEGVAKPDGTLHPMQAAFKENHGLQCG
                     FCTPGMVMNALDFAKSHPNPTEQEVREALEGNLCRCTGYHNIVKSIIAGARAMGGKI"
BASE COUNT          420 a          917 c          964 g          527 t
ORIGIN      
        1 tcttcttgtc ggtgcccgcg accttgaact cgcccttgtc gtactcgatg tcggcttccg
       61 acgcttccag caggtgcgcc gcaatcttct tgcccttggc gacgatcttg tcgagcgcct
      121 tgacaatcgc ggtgccaccc acggcgatgg agcgcgagcc gtaggtgccc atgccgaaga
      181 ggatcttgga agtgtcgccg tgcaccacct cgaccgcctc gatcggcagg ccgagccggt
      241 cggcgaccac ctgcgcaaag ctcgtctcgt ggccctggcc atgcgaatgc gagccggtga
      301 acacggtgac ggtgcctgtc ggatgcacgc ggatctcgcc cgcctcgaac aggcccgccc
      361 gcgcgccgag ggagccggcg acgttggagg gcgcgatgcc gcaggcttcg atgtaacagg
      421 catagccgat gccgcgcagc ttgccgtgct ttgccgactc ttcttttctc ttgctgaagt
      481 tctgaacgtc cgcgagcttg agcgctgcat ccagggtggc ctggtagttc ccggtgtcgt
      541 agcagagcgc gaccggcgtc tggtacggga actcgcgaat gaagttttgc tttcggatct
      601 cgaccgggct catcttcatc tcgcgcgccg cagtctcgac caggcgctcg accacgtagg
      661 tcgcttccgg gcggccggcg ccgcgatagg cgtcgacggg cgtggtgttg gtgaacaccg
      721 cggtcacctc gcagtagatg tgcggcgtcg tgtactgccc ggcgagcaag gtcgcgtaca
      781 ggatggtcgg gatgcacgag gcgaaggtcg agaggtacgc gccgaggttc gcggtcgtgc
      841 gcacgcgcat cgcgaggaac ttgccgtcct tgtcgagcgc gagctcggcg ctcgtcaggt
      901 ggtcgcgccc gtgcgcgtcg gagacgtagg actccgagcg ctcggcggtc cacttcaccg
      961 ggcggttcag ttgtttcgcc gcccaggtga ccaccacgtc ctcgccgtag aggtagatct
     1021 tcgagccgaa gccgccgccg acgtcgggcg aaaccacgcg caccttgctc tcgggcagcc
     1081 cgagcacgaa cgcggtcatc agcagccgct cgaccacgta ggtggcctcc ggccgcccgg
     1141 cgccgcggta cgcgtccacc ggcgcggtgt tggtgaacac cgcggtgacc tcgcaataga
     1201 tgagcggggt ggtgtactgg ccggcgagca gcgtcgcgta gaggatggtc gggatgcacg
     1261 aggcgaacgt cgaaaggtac gcgccgaggt tcgccgtggt gcgcacgcgc atcgccagga
     1321 acttgccgtc cttgtcgagc gcaagctccg cggtggtgag gtggtcgcgc ccatgcgcgt
     1381 cggagacata cgactccgag cgctcggcgg tccacttcac cgggcggttc agctgcttcg
     1441 ccgcccaggt gacgaccacg tcctcgccgt agagatagat cttcgagccg aagccgccgc
     1501 cgacatcggg cgccaccacg cgcaccttgc tctcggggag cccgagcacg aacgccgtca
     1561 tgagcagccg ctcgacgtgc gggttctggc tcgcgacgta gagcgtgtag ccgtcgtcgg
     1621 cgcgcgagta caccgcgttc gccgagcgcg gctcgatcgc gttcggcacc aggcggttgt
     1681 tccagaaatc gagcttggtc acatgggctg cttttgcgaa cgcagcctcg accgcggctt
     1741 tgtcgcccag cgcccagacg tagcaggtgt tgtccggcgc gagctcgtga atcgcgggac
     1801 cgttcctcgc cttgcccacg tcggcgactg cgggaagctc ctcgtagtcg acttgtatct
     1861 tctccgcggc gtcgcgcgcc tgctcgggcg tctgggcaac caccacggcg acgcgctcgc
     1921 cgacatggcg caccttgccc tgcgcgagcg gcgggtaggg cggctccttc atcggcttgc
     1981 cgttgacgtc cgtgatgagc cagccgcacg gcaggccgcc gaccttggcg gccgccaggt
     2041 cttcgccggt gtacacggcg agaacaccgg aggatgttct cgctgtctcg gacgagatgt
     2101 tcctgatctt cgcgtgcgcg tgcggcgaac gcacgaatac ggcgtaggtc tggttgggca
     2161 gggcaacgtc gtcggtgaat tgcccgctcc ccgtgaggaa acgatagtcc tccttgcgct
     2221 ggacgcgcgc gccgatcagg ggggcgttca tatcttgcct cccattgcgc gcgcgccggc
     2281 gatgatgctt ttcacgatgt tgtggtagcc ggtgcagcgg cacaggttgc cctcgagcgc
     2341 ctcgcgcacc tcctgctcgg tggggttggg atggcttttc gcgaagtcga gcgcgttcat
     2401 caccatgccc ggggtgcaga aaccgcactg caggccgtgg ttctccttga acgcggcctg
     2461 catcgggtgc agggtgccgt cgggcttcgc cacgccctcg atggtcatga tgtcggcgcc
     2521 ctcggcctgc atcgcaacaa tcgagcagct cttgatcgcg cggccgttca tcagcaccgt
     2581 acaggcgccg cactgcgccg tgtcgcagcc gacgtgggtg ccggtcagcc gcaggttctc
     2641 gcggatatag gtgaccagca gcgtgcgcgc ctcgagctcg gcggaaacag gcttgccatt
     2701 gaccttcatg gatacgttga ttgtcatgcg gggctcctcc gggggagaaa aatatcgtag
     2761 cgcggcggta tgatttatgc cacacccatt tctcgccggg cggccatgcg attgctgatc
     2821 gtggagga
//
LOCUS       DASUNT010000457         5767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_415894, whole genome shotgun sequence.
ACCESSION   DASUNT010000457 DASUNT010000000
VERSION     DASUNT010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415894"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VFX94_09245"
     CDS             <1..413
                     /locus_tag="VFX94_09245"
                     /inference="COORDINATES: protein motif:HMM:NF013177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEX5768406.1"
                     /translation="FFFMHAPRLVEAGHLYVAQPPLYKVEVPSQGKGRPARRIYCLDD
                     DELEERLASLKKEKVKEGSWEISRFKGLGEMSPEQLWETTMNPDARRLIRMEFSAKNF
                     SKAKETFEKLMDAGEAEERRNWMREHWKSVEADV"
     gene            397..633
                     /locus_tag="VFX94_09250"
     CDS             397..633
                     /locus_tag="VFX94_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768407.1"
                     /translation="MKRTSRKNKPFWERAYRGHAYWLGKHKLGKVTLLAEHPKGKYQW
                     EAGGRAGFSDELEKAKAAVERAALNADKQLDLFK"
     gene            638..2932
                     /gene="parC"
                     /locus_tag="VFX94_09255"
     CDS             638..2932
                     /gene="parC"
                     /locus_tag="VFX94_09255"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX5768408.1"
                     /translation="MDDRTGDLFTSNGDDAAPIEEHASHAYLGYAVSTVKARALPEIA
                     DGLKPVQRRILYAMNDAGAQGFAKCARYVGEVLGKYHPHGDSSTYEAMVHLAQPFSMR
                     YPLIDGQGNFGSRDGDAPAAYRYTEARLSRFAGLLLSEIGEGTVDFIRNYDGKFDEPV
                     LLPARLPFGLLNGSFGIPVGFSTRIPSHNLKEVAAAAAHLIRHPRARLEDVLDIMPGP
                     DFPGGGQIISPKEEISEAYASGRGSLLVRCRWEKEPLARGQWRVVVTELPHGVSVKQV
                     MEEIEALANPKAQGSRKEVSQEQRRMKQFILDQVESVRDESDRRSKLRLVIEPRSSRQ
                     DENELMAALMVHTALETRYPVNLTWLGLDGLPETKGLVEILREWGEFRVRTVRRRTEF
                     RLAKCESRLHIVEGRLKAFARIDDIIKLIRASEDQAEAKEKLREKFKFTEKQAEDVVN
                     LRLGQLTRLDGVALNDERKALEAERKGLKGILGDEKELKKLVVKELEEDAKKFGDERR
                     TLIKTAERAQVERTVVEEPVSVILSRKGWIRARTGHGLDVTQLSFKDGDALLQTLECK
                     TTDPVTVLAASGKTFTVAAADIPSGRGDGAHVNTLVNSGSDDIVWMGSGSAQTRLLMN
                     SSAGLGFLCRLGDLATKTRQGKDFMKIEEGARANAPAAIAEGSAYVAALSSDSRLLLF
                     PLDEVPERPNGGVGVQLLALPEKQTLASVAATDGKSLVVSGIKRKNRTFEALDAKQLA
                     AHVGKRAQRGRLADIGLRADRLGS"
     gene            2997..4175
                     /locus_tag="VFX94_09260"
     CDS             2997..4175
                     /locus_tag="VFX94_09260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5768409.1"
                     /translation="MRELQRAVRPFAIPAAILGIAGVGHLVGPPMPESLEGLWILGPY
                     ALLATAVVLSWVFNRGRAWVIAASLLCAFAVQQLYAGRVVYTVLVVLVPFNALLAMVL
                     AERGARYRAAYGWLLLLACEAALIVGISTVEAAALDNVLLRSPPTPLAGRLAFAAAFA
                     AAVWRAWPEHTPLQVGNAGALVAFYIAAEWVGAPQVYSTFMACAGVILIVALVQESHR
                     LAFRDTLTGLPGRRALDERLRSLDRDYVLAMVDVDHFKEFNDTHGHDIGDQVLKLVGA
                     RLAEVGGGGIAFRYGGEEFCVVFPDAELEDVLLQLEAIRVSIEGYRMAVRAPDRPKEL
                     ELGAKRRGEGASDKHLSVTVSIGACRPSASLRNPAQVIKGADEALYRAKQAGRNRVST
                     "
     gene            4479..4886
                     /gene="arfB"
                     /locus_tag="VFX94_09265"
     CDS             4479..4886
                     /gene="arfB"
                     /locus_tag="VFX94_09265"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX5768410.1"
                     /translation="MAAPSLVVPEHEVELTAVRAQGAGGQNVNKVSNAVHLRFDIRAS
                     SLPQHVKERLLRTGDQRVSRTGVVVIKAQTHRSLERNRAEALARLQSLVTAAAHLPKP
                     RKPTRPTRASKVARVEAKKRHSRLKALRGKVLD"
     gene            4883..>5767
                     /locus_tag="VFX94_09270"
     CDS             4883..>5767
                     /locus_tag="VFX94_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX5768411.1"
                     /translation="MKDILAAVIAAVEAEGERLAAEFMRADGPRGRRGSAPIDREMEE
                     RLRETLLRLLSCAFVGEETGVSGNVSDSVWLVDPHDGTFEFTGGKRGSAVSVALLKKS
                     VPVLGVVHSPLSPDCGRDTIAWAQGAGPIRRNGKALEVDLSHRRLAAGEFVFATGSSA
                     LRPGAWSRACAPARYIALPSIAYRLARVAAGDGVATVSIHGVNEYDVAAGIALVRAAG
                     GVVLDAQGKEVALTGEAGRRLSGCVAGAPAAAAQLSAHDWKSLEDEPQQDPRVALGFP
                     RTTPPARLDRAAACLLGQV"
BASE COUNT          986 a         1932 c         2001 g          848 t
ORIGIN      
        1 cctttttctt catgcacgcg ccgcgcctcg tcgaagcggg ccacctctac gtcgcgcagc
       61 cgccgctcta caaggtcgag gtgccgagcc agggcaaggg caggcccgcg cggcgcatct
      121 attgcctgga cgacgacgag ctcgaagaaa ggctcgcctc gctcaagaag gaaaaagtga
      181 aggagggcag ctgggagatc tcgcgcttca agggcctggg ggagatgagc cccgagcagc
      241 tctgggaaac caccatgaac cccgatgcgc ggcggctcat acggatggag ttttctgcga
      301 agaatttctc caaggcaaag gagacattcg agaagctgat ggacgccggc gaggccgagg
      361 agcggcgcaa ctggatgcgg gagcactgga agagcgttga agcggacgtc taggaagaac
      421 aagcccttct gggagagggc gtatcgcgga catgcttact ggctcggaaa gcacaagctc
      481 gggaaggtga cgcttctcgc ggagcacccc aaagggaaat atcagtggga agccggcggc
      541 agggcgggct tctccgatga gctggaaaaa gcaaaggcag cagtcgagcg cgcggcattg
      601 aacgcggaca aacaactgga cctcttcaag taacgccatg gacgacagaa ccggagatct
      661 cttcaccagc aacggcgacg acgcggcgcc catcgaggag cacgcctcgc acgcctacct
      721 cggctacgcc gtctcgacgg taaaggcgcg tgcgctgccc gagatcgccg acgggctgaa
      781 gccggtgcag cggcgcatcc tgtacgcgat gaacgacgcg ggcgcgcaag gcttcgccaa
      841 atgcgcccgc tacgtcggcg aggtgctcgg caagtaccac ccgcacggcg acagctccac
      901 ctacgaggcg atggtgcacc tcgcgcagcc cttctcgatg cgctacccgc tgatcgacgg
      961 ccaggggaac ttcggttccc gtgatggcga tgcgcccgcg gcgtatcgct atacggaagc
     1021 aaggctgtcc cgattcgccg ggctcctgtt gagcgaaatc ggggagggca ccgtcgattt
     1081 catcaggaac tacgacggga aattcgacga gcccgtgctc ctgcccgcgc ggctgccgtt
     1141 cgggctgttg aacggcagct tcggcatccc ggtcggcttc tccacgcgca tcccgtcgca
     1201 caacctgaag gaagtcgccg ccgctgccgc gcacctcatc aggcacccgc gcgccaggct
     1261 cgaggacgtg ctcgacatca tgcccggtcc ggatttcccc ggcggcgggc agatcatctc
     1321 gccgaaggaa gagatcagcg aagcgtacgc gagcggtcgc ggctcgctcc tcgtgcgctg
     1381 cagatgggag aaggagccgc tcgcccgcgg tcagtggcgc gtcgtcgtga ccgagttgcc
     1441 gcacggcgtg tcggtaaagc aggtgatgga agaaatcgag gccctcgcca acccgaaagc
     1501 ccaggggagc aggaaggaag tctcgcagga gcagcgcagg atgaagcagt tcatcctcga
     1561 ccaggtcgag tcggtgcgtg acgagagcga ccgcaggtcg aagctgcgcc tcgtgatcga
     1621 gccgaggagc tcgcggcagg acgagaacga gctgatggcc gcgctcatgg tgcacaccgc
     1681 gctcgagacg cgctatccgg tgaacctcac ctggctcggc ctcgacggcc tgcccgagac
     1741 caagggcctc gtcgagatcc tgcgcgagtg gggcgagttc cgcgtgcgca cggtgcgccg
     1801 gcgcacggag tttcgcctgg cgaagtgcga gtcgcgcctg cacatcgtcg agggccggct
     1861 caaggccttc gccaggatcg acgacatcat caagctcatc cgggcgagcg aagaccaggc
     1921 cgaggcaaag gaaaagctcc gcgagaagtt caagttcacc gaaaagcagg ccgaagacgt
     1981 cgtcaacctg cgcctgggcc agctcaccag gctcgacggc gtcgcgctga acgacgagcg
     2041 caaggcgctc gaagccgagc gcaagggact aaagggcatc ctcggcgacg agaaggagct
     2101 gaagaagctc gtcgtcaagg agctcgagga ggatgcgaag aagttcggcg acgaaaggcg
     2161 cacgctgatc aagaccgcgg agcgcgcgca ggtggagcgc acggtggtgg aggagccggt
     2221 cagcgtgatc ctgtcgcgca aaggctggat ccgcgcgcgc accggccatg ggctcgacgt
     2281 cacccagctc tcgttcaagg acggcgatgc gctgctgcag acgctcgaat gcaagaccac
     2341 cgaccccgtg acggtgctcg ccgcgtcggg aaagacgttc accgtcgccg ccgccgacat
     2401 cccgtccggg cggggcgatg gagcgcacgt gaatacgctc gtcaactccg gctccgacga
     2461 catcgtgtgg atgggctcgg gcagcgcgca gacgcgcctt ctcatgaaca gctccgcggg
     2521 cctgggcttc ctctgcaggc tcggcgacct cgcgaccaag acgcgccagg gcaaggactt
     2581 catgaagatc gaagagggcg cgagggccaa tgctccggcc gccattgcgg agggctcggc
     2641 ctatgtcgcc gccctgtcgt ccgattcccg cctgcttctc ttccccctcg atgaagtgcc
     2701 cgagcgcccc aacggcggcg tgggcgtgca gctcctcgcc ctgccggaaa aacagacgct
     2761 cgcatcggtg gccgctacgg acggaaagtc gctcgtggtg tcgggaatca agcggaagaa
     2821 ccgcacgttc gaagcgctgg acgcgaaaca gctcgcggca catgttggca agcgcgcgca
     2881 gcgcggcagg ctggccgaca tcggcttgcg tgccgatcgc ctgggaagct gaagaactgc
     2941 acgtttctta tcccgccgtc tcggggaaat cgggcaaaag ccgttacgat ttgccgatgc
     3001 gcgagcttca gcgcgccgtg cggccgtttg ccattccggc ggcgattcta gggatcgcgg
     3061 gagtcggcca ccttgtcgga ccgccaatgc ccgaatcgct cgagggcctc tggatcctcg
     3121 ggccgtacgc gctgctcgcg accgcggtgg tgctctcctg ggtgttcaac cgcggccgcg
     3181 cctgggtcat cgcggcctcg ctcctttgcg cgttcgccgt gcagcaactc tatgcgggca
     3241 gggttgttta tacggtgctc gtggtgctcg tgcccttcaa cgcgctgctc gccatggtgc
     3301 tcgccgagcg cggcgcacgc taccgcgccg cgtacggctg gctgctgctc ctcgcgtgcg
     3361 aggcggcgct gatcgtcggc atttcgacgg tggaagccgc ggcgctcgat aacgtgctgt
     3421 tgcgctcgcc gccgacgccg ctcgccggcc ggctcgcctt cgcggctgcg tttgccgctg
     3481 cggtgtggcg cgcgtggccg gagcacacgc cgctgcaggt cggcaacgcg ggcgcgctcg
     3541 tcgccttcta catcgccgcc gaatgggtcg gcgcgccgca ggtgtattcc accttcatgg
     3601 catgcgccgg tgtgatcctg atcgtcgcgc tggtgcagga gtcccaccgg ctcgcgttcc
     3661 gcgacacgct taccggactc cccggccgcc gcgcgctgga cgagcgcctg cgcagcctcg
     3721 accgcgacta cgtcctcgcg atggtcgacg tcgaccactt caaggaattc aacgacaccc
     3781 acggccacga catcggcgac caggtgctga agctggtcgg cgcgcggctt gccgaggtgg
     3841 gcggcggcgg catcgcgttt cgctatggcg gcgaggagtt ctgcgtcgtg tttcccgatg
     3901 cggagctcga ggacgtcctg ctgcagctcg aggccatccg cgtctcgatc gaaggctacc
     3961 gcatggcggt gcgcgcgccc gaccgcccga aggagctcga gctcggcgcc aagcgccggg
     4021 gcgagggcgc ctccgacaag cacctttcgg tcaccgtctc catcggcgcg tgccgcccga
     4081 gtgcgagcct gcgcaacccc gcacaggtca tcaaaggcgc cgacgaggcc ctctaccgcg
     4141 caaagcaggc cggccgcaac cgggtcagca cataacttgc ttttcttgaa tagtcatgcg
     4201 cacgctctca gcaatggttt tgatgtcgtt ggccttcgcc gccgcagcgc aggaccagcg
     4261 gcaaagcgac aaggacacgc aagctcgcat gcggagcgaa ggctcggccg gcggcctggg
     4321 aaacgtaacg cccgagcaga aggctggcgc cgacgtgggt gccgggccgc acctcgagcg
     4381 caagcccggc ggcgagtcga aacgcgagca ccaggagaag tcctccgagc gtgaagcagg
     4441 ccgaggcgcg actcgcggcg aggcgctaca gtccgggaat ggccgcgccg agcctcgtcg
     4501 tccctgagca cgaagtcgag ttgaccgccg tgcgcgcgca gggcgcaggc gggcagaacg
     4561 tcaacaaggt ttcgaacgcg gtccacctgc gcttcgacat ccgtgcctcg tcgctgccgc
     4621 agcacgtgaa ggagcggctc ctgcgcaccg gcgaccagcg cgtgagccgc accggggtgg
     4681 tcgtgatcaa ggcgcagacg caccgcagcc tcgagcgcaa ccgcgccgag gcgctcgcgc
     4741 ggctgcaatc gctcgtcacc gcggccgcgc acctgccgaa gccgcgcaag cccacgcgcc
     4801 ccacgcgcgc ctccaaggtc gcgcgcgtgg aagcgaagaa gcggcattcg cgcctcaagg
     4861 cgctgcgcgg caaggtcctc gattgaaaga catcctcgct gcggtgatcg ccgcggtcga
     4921 ggcggagggc gagcgcctcg ccgcggagtt catgcgcgcc gacggcccgc gtggccggcg
     4981 cggctccgcg ccgatcgacc gggagatgga agagcggctg cgcgagacgc tgctgcgcct
     5041 cttgtcgtgc gccttcgtgg gcgaagagac gggggtatct gggaacgtgt ccgatagcgt
     5101 ctggctcgtc gacccgcacg acggcacctt cgagttcacc ggcggcaagc gcggctcggc
     5161 cgtttccgtc gctctgctca agaaaagcgt accggttctc ggcgtggtgc acagcccgct
     5221 ttcgcccgac tgcggccgcg acacgatagc ctgggcgcag ggggccgggc cgatccgccg
     5281 caacggcaag gcgctcgagg tggatctttc gcacaggcgt cttgccgccg gcgagttcgt
     5341 gttcgcgacc ggctcctcgg cgctgcgccc cggcgcgtgg tcgcgcgcct gcgcgcccgc
     5401 gcgctacatc gcgctgccga gcatcgccta ccgcctcgcg cgcgtcgctg cgggcgacgg
     5461 cgtcgccacg gtttcgatcc acggcgtgaa cgagtacgac gttgccgcgg gcatcgccct
     5521 cgtacgggcg gcgggcgggg tggtgctcga cgcgcagggc aaggaagtcg cgctcaccgg
     5581 cgaggccggc cgccggctct ccggctgtgt cgccggggcg cccgccgccg cggcgcagct
     5641 ctccgcgcac gactggaagt ctctcgagga cgagccgcag caggatccgc gcgtcgcgct
     5701 cggctttccg cgcaccacgc cgcccgcgcg cctggaccgc gcggcggcct gcctgctcgg
     5761 ccaggtg
//
LOCUS       DASUNT010000458         2430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_589871, whole genome shotgun sequence.
ACCESSION   DASUNT010000458 DASUNT010000000
VERSION     DASUNT010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589871"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFX94_09275"
     CDS             <1..249
                     /locus_tag="VFX94_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768412.1"
                     /translation="RIRVKVNAADQRRPDFKLTLGLRGLLLLVDEDDFVVAALLFVHR
                     LPAASRASSSVIVCHSDRNQHGSAASFALMSASNVFFS"
     gene            514..612
                     /gene="ffs"
                     /locus_tag="VFX94_09280"
     ncRNA           514..612
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFX94_09280"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            629..2056
                     /gene="dnaX"
                     /locus_tag="VFX94_09285"
                     /pseudo
     CDS             629..2056
                     /gene="dnaX"
                     /locus_tag="VFX94_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749005.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
     gene            2145..>2430
                     /locus_tag="VFX94_09290"
     CDS             2145..>2430
                     /locus_tag="VFX94_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX5768413.1"
                     /translation="MKGNIGQLMKQAQQMQENMRRMQESLATTEVEGQSGAGMVKVQM
                     TCKYEVKRVSIDPSLVGDDREMLEDLVAAAFNDAARHVEATIAEKMSGMTA"
BASE COUNT          431 a          766 c          833 g          400 t
ORIGIN      
        1 aggatacgag taaaggttaa cgccgccgac caacggcgtc ctgacttcaa gctgacgctt
       61 ggcttacgag ggctgcttct tctcgtagac gaggacgatt tcgtggtcgc cgcgcttctc
      121 ttcgtgcacc ggttgccggc agcctcgcgc gcctcctcca gcgtcattgt ttgccacagc
      181 gatcgtaatc agcacggcag cgcggcgtcc ttcgcgctca tgtcggcgtc gaatgttttc
      241 ttttcgtaga cgagaacggt ctcgccgggc gtgcgcgctt cctggcgcgc gagcttgcct
      301 gacgcggcga tcgcttcgtc gagactcttg ccggcaacct tgcgctcgaa cgagcctcgg
      361 gtcgggccgt aggcgccggc ggcaagtgtc agcgtcgcga ttgcgatgaa aatggtctgt
      421 ttccagttca gcagccgtct ggcgcgaagg gttttccggt caggggctca ctgcgccctg
      481 acgcagcctt acggatgact tacaatcgca ggcggcgggc ctccccgcat tgcagtctgg
      541 tgaacctggt caggtccgga aggaagcagc cacagccggt cgctgcgagt gccgggggtc
      601 gggctcgcca ccttactcga ttcgagcgat gagctatcaa gtccttgccc gcaaatggcg
      661 gccgcgggat ttcggcagcc ttgtggggca ggagcacgtg gtgcgcgccc tgcggcacgc
      721 gctcgaacac aagcgcctgc atcatgccta cctctttacc ggcacacgtg gcgtgggcaa
      781 gaccacgctt gcgcgaattc tcgccaagtg cctcaactgc gagacgggca tcacgccgca
      841 gccgtgcaac gcctgcccgg cgtgcgtgga aatcgacgcc gggcgattcg tcgaccttct
      901 ggaagtcgac gcggccacca acacgaaggt ggacgagatg cgccagctgc tcgacaccgc
      961 gcagtacgcg ccgacccgcg cgcgtttcaa ggtctacgtg atcgacgagg tgcaccagct
     1021 ctcgggacac gccttcaacg cgatgttgaa gacgctggag gaaccgcccg agcacatgaa
     1081 gttcatcctg gccacgaccg atccgcagaa gatcccggtg accgtgctca gccgctgcct
     1141 ccagttcaac ttgaagcaga tgccgcgcga ggcgatcgcc tctcacttga agctggtctt
     1201 ggaaaaggaa caaatcgcat cggagccgga ggcgctttcg ctgctcgccc gcgcggctgc
     1261 gggcagcatg cgcgacgcgc tgtcgctgct cgaccaggcg atcgcacacg gcggcggccg
     1321 cgtgacggcg gcgagcgcgg ccgacatgct cggcgccatt gaccagacgc acctggtacg
     1381 gctcgtggag gcgattgccg caggcgatgc ggccgccgcg gtaaagaccg ccgacgagat
     1441 gcaggcgcgt agcctttctt tcgacgctgc gctgaccgag ctggcgagcc tgctgctgca
     1501 gcttgcgctg gcgcagtcgg tgcccggtgc ggtcgatgat gggcgggtca aggagcttgc
     1561 ggcccggctc gacccggaat ccgtgcagct ctggtaccag atcgcgctcc agggcaggga
     1621 agacctgccg ctcgcgccgg acgagcatgg gggcttcgta atgacggtcc tcagaatgct
     1681 tgcgtttcgt ccggaagggg cgggcaagga gctgcccgcg cccaagaaga gcgcgagcgt
     1741 tccccgcagc ccgcgcagcg gcgattggcc cgagctggta cagcagctgc ctgtcagcgg
     1801 agcggcgcgc gagctcgcgc gcaacgccga gctcaggcgc cgcgagggcg cgacgtttga
     1861 gctcgtcgtt ccgaaagcga aggcctatct cgcggagcgc agctacgtcg acaagctcaa
     1921 ggccgcgctc gagcagcagc tcggcgcgcc ggtaatcctc aaggtatcgg cgggggagat
     1981 cgccggcgcg agcgtcgcgt cgctcgaggc gggcgagcgc gaagcgcgcc gcgccgccgc
     2041 ggcgcaggcg gtggcgagcg acggtttcgt gcaggatctc gtcaacctgt tcgacggcaa
     2101 ggtcgtcgat tccaccatcc gggaagatcg aaagtgagcg gcttatgaaa ggcaacatcg
     2161 gccagctgat gaagcaggct cagcagatgc aggagaacat gcgccgcatg caggagagcc
     2221 tcgccaccac cgaagtggaa ggccagtccg gcgccggcat ggtgaaggtg cagatgacct
     2281 gcaagtatga agtgaaacgg gtgagcatcg atccttcgct ggttggcgac gaccgcgaga
     2341 tgctggaaga cctggttgcc gcggccttca acgacgcggc acgccatgtc gaggcgacga
     2401 tcgcggaaaa gatgagcggg atgacggccg
//
LOCUS       DASUNT010000459         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_503544, whole genome shotgun sequence.
ACCESSION   DASUNT010000459 DASUNT010000000
VERSION     DASUNT010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503544"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VFX94_09295"
     CDS             <1..245
                     /locus_tag="VFX94_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009931004.1"
                     /note="catalyzes the reversible formation of glyoxylate
                     and succinate from isocitrate; glyoxylate bypass pathway;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEX5768414.1"
                     /translation="LARGYRERGMAAYSELQQAEFASEQYGYTATRHQREVGTGYFDE
                     ITQVVTAGKASTTALHGSTEDEQFFDSQKKPKLAAA"
     gene            260..1969
                     /gene="aceK"
                     /locus_tag="VFX94_09300"
     CDS             260..1969
                     /gene="aceK"
                     /locus_tag="VFX94_09300"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HEX5768415.1"
                     /translation="MARTILEGFDRHYRLFREAAIEARRLFERAAWREMQALARSRIQ
                     MYDTRVDEAVRALTDRYPEAETDESLWPAIKAAYIDLIQDHKQPECAETFYNSVACAV
                     LHRRYYKNEFIFWRPAIATEHLEGEAPSYRCHYPLRGGLRTTLRDIATGFGLANPWED
                     LSRDVRNIVRALRQHFPRPARAQPDLQIQVLASLFFRNKAAYIIGRAVNAHRELPFAV
                     PVLQNERGELYLDTLLVGEDQLLVLFSFARAYFLVDMEVPAAYVSFLRWLMPRKPRAE
                     MYMAVGLAKQGKTLFYRDLHYHLKHSSDRFTVAPGIRGMVMLVFTLPSFPYVFKVIRD
                     RFAPPKDVDRQTVLEKYLMVKLHDRVGRMADTLEYSLVALPLDRFDPALLEELRRECV
                     STVEVDGESLVLGHVYIERRMQPLNLHVEECRRDGDEARLRYALREYGNAIKELAGAG
                     IFPGDMLLKNFGVTRHDRVVFYDYDEIQPITDVTFRRVPAASSYEEELAAEPYWRVGE
                     GDVFPEQFEYFLVSNPRAREIFYEYHRDLLDPQFWTTKQQRFQDGVLEDVFPYPEEIR
                     FRR"
     gene            complement(1953..>2260)
                     /locus_tag="VFX94_09305"
     CDS             complement(1953..>2260)
                     /locus_tag="VFX94_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX5768416.1"
                     /translation="PAYPSSNPVRGAHRGEPPARCGGPGLILELHVQPGAASTGFAGK
                     HGERLKVRLQARAVDGKANEALIDFLAGHFGVPRRSVRILSGLKSRQKRVEILSAGT"
BASE COUNT          397 a          778 c          705 g          380 t
ORIGIN      
        1 agctggcgcg cggataccgc gagcgcggca tggcggccta ctccgagcta cagcaggccg
       61 agttcgccag cgagcaatat ggctataccg ccacccggca ccagcgcgag gtcggcaccg
      121 gctacttcga cgagatcacg caggtcgtga ccgcgggcaa ggcctccact acggcgctgc
      181 acggctcgac cgaggacgag caattcttcg actcgcagaa aaagccgaag ctcgccgcgg
      241 cgtagatcac gcctcgagga tcgcccgcac gatcctcgaa ggcttcgacc ggcactatcg
      301 tctgttccgc gaggcggcga tcgaggcgcg ccgcctcttc gagcgcgcag catggcgcga
      361 gatgcaggcg ctcgcgcgca gccgcatcca gatgtacgac acgcgcgtcg acgaagcggt
      421 gcgcgcgctt accgaccgct atcccgaggc ggagaccgac gagtcgctgt ggccggcgat
      481 caaggcggcc tacatcgacc tcatccagga ccacaagcag cccgagtgcg ccgagacctt
      541 ctacaactcg gtcgcgtgcg ccgtgctgca ccggcgctat tacaagaacg agttcatctt
      601 ctggcggccc gcgatcgcca ccgagcacct cgaaggcgaa gcgccgagct accgctgcca
      661 ttacccgctg cgcggcggct tgcgcaccac gctgcgcgac atcgcgaccg gcttcggcct
      721 cgccaacccc tgggaggacc tttcgaggga tgttcgcaat atcgtgcgcg ccctgcgcca
      781 gcatttcccg cggcccgcgc gcgcgcagcc cgacctccag atccaggtgc tcgcgtcgct
      841 tttcttccgc aacaaggcgg cgtacatcat cggccgggca gtcaacgcgc atcgcgagct
      901 gccgttcgcg gtgccggtcc tgcagaacga gcgcggcgag ctctacctcg acacgctgct
      961 ggtcggcgag gaccagctcc tcgtgctctt ctcgtttgcg cgcgcctact ttcttgtcga
     1021 catggaggtt ccggcggcct acgtctcgtt cctgcgctgg ctcatgccgc gcaagccgcg
     1081 cgccgagatg tacatggcgg tcgggctggc gaagcagggc aagacgctct tctaccgcga
     1141 tctgcattac cacctcaagc attcgagcga ccgcttcacg gtggcacccg ggatcagggg
     1201 gatggtgatg ctcgtcttca cgctgccgtc tttcccctac gtgttcaagg tgatccgcga
     1261 tcgcttcgcg ccgcccaagg acgtcgaccg gcagacggtg ctcgaaaagt accttatggt
     1321 gaagctccac gaccgcgtcg ggcgcatggc cgataccctg gagtactcgc tcgtcgccct
     1381 gcctttggac cgtttcgatc ccgcactgct ggaggagctt cgccgcgagt gcgtttccac
     1441 cgtcgaagtc gacggcgaga gcctggtcct cgggcatgtg tacatcgagc ggcgcatgca
     1501 gccgctcaac ctgcacgtcg aggaatgccg tcgcgacggc gacgaagcgc ggctgcgcta
     1561 tgccctgcgc gagtacggca atgcgatcaa ggagctcgcc ggggccggca tcttccccgg
     1621 cgacatgctg ctcaagaact tcggcgtgac gcgccacgat cgcgtcgtct tctacgacta
     1681 cgacgagatc cagccgatca cggatgtgac attccgccgc gtgccggccg cttcgagcta
     1741 cgaagaggag cttgccgccg agccgtattg gcgcgtgggc gaaggcgacg tgtttcccga
     1801 gcagttcgag tacttcctcg tgagcaaccc gcgcgcccgc gagatcttct acgaatacca
     1861 ccgcgacctg ctcgatccgc agttctggac cacgaagcag cagcgcttcc aggacggcgt
     1921 gctggaagac gtttttccct atcccgagga aatcaggttc cggcgctgag gatctcgacg
     1981 cgcttctggc gcgacttcag tcccgagaga atacgcacgc tgcgccgcgg cacgccgaaa
     2041 tgcccggcga gaaagtcaat cagagcctca ttggccttgc catcgaccgc gcgcgcctga
     2101 agcctgacct tgaggcgctc gccgtgcttg ccggcgaacc cggtgctcgc ggcccccggc
     2161 tggacatgca gctccaggat caaacctggg ccgccgcaac gcgcaggtgg ttcaccacga
     2221 tgagcaccac gtacaggatt actagaagga taagcggggt
//
LOCUS       DASUNT010000460         3894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_240519, whole genome shotgun sequence.
ACCESSION   DASUNT010000460 DASUNT010000000
VERSION     DASUNT010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240519"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="VFX94_09310"
     CDS             complement(<1..1383)
                     /locus_tag="VFX94_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX5768417.1"
                     /translation="MGKVTGFLEHERLEEPHEPVEARKKHYREFYLRLSDEAAGIQGA
                     RCMDCGIPFCMSGCPVNNIIPDWNDLVYRQDWKAAIEVLHSTNNFPEFTGRICPAPCE
                     EACVLRINADPVGIKSIEHAIIDKAWEEGWVQPQPAAHKSGKQVAIVGSGGAGLACAQ
                     QLARAGHAVTVFEKSDRIGGLLRYGIPDFKLEKAVIDRRLEQLRAEGVKFRTAVFVGV
                     QRPGSAIPNDAHQNIAPQKLLAEFDAIVLAGGAEQPRDLPIPGRGLDGVHFAMEFLPL
                     QNKRVAGDAGIRDLSAAGKHVVIIGGGDTGSDCVGTSNRHGARSITQFELLPMPPDVE
                     RNPVWPYWPTRLRTSSSHEEGVNRDWSIATKEFLGKNAKVEALRAVRLEWKDGKPHEV
                     PGSAFEMPAELVLLAMGFVSPLQSLLEEFGLQRDERGNARAPTEGPGAYATSVPKVFA
                     AGDMRRGQSLV"
     gene            complement(1397..>3894)
                     /locus_tag="VFX94_09315"
     CDS             complement(1397..>3894)
                     /locus_tag="VFX94_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEX5768418.1"
                     /translation="ELAEGGKLGAALDGGKACKNFIKAIGKGLRKVMSKMGISTYMSY
                     TGSQIFEAVGLAKSLVDKYFTGTASNVEGIDLFDLAEEAIRIHRAAFEERDPVLASML
                     DAGGEYAWRVRGEDHTWTPDAIAKLQHASRANSYATYKEYAAIINDQSRRHMTFRGLF
                     EFRSGLCPPVPIEEVEPAAEIVKRFSTGAMSLGSISTEAHTTLAIAMNRIGGRSNTGE
                     GGEDRRRYAPVKAGESLQGRLGKSRVEVDIPLREGDSLKSKIKQVASGRFGVTAEYLA
                     SAEMLQIKIAQGAKPGEGGQLPGRKVSEYIAELRYSTPGVELISPPPHHDIYSIEDLA
                     QLIHDLKNSNPNAAISVKLVSEVGVGTIAAGVSKGKSDHVTIAGHDGGTGASPWSSIK
                     HAGTPWELGLAETQQTLVLNRLRGRIAVQVDGQLKTGRDVVIGAILGADEFGFATAPL
                     VVEGCIMMRKCHLNTCPVGVATQDPVLRRKFEGKPEHVVNYFFHVAEEAREIMAQLGV
                     RRLNELIGRTEFLDTKNGIEHWKAKGLDFSRVFAMPKMPPEVSRFHDEVQHHGLEKAL
                     DNRLIELARPALERGEKVAIETPIRNINRTVGTMLSYEIAKRYGHDGLPDDTVHIRLA
                     GSAGQSFGAFLAKGVTLDLVGDTNDYCGKGLSGGRISVQPSPKFRGEPTENIITGNVV
                     LYGAIAGEAYFRGVAGERFAVRNSGARAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAA
                     GMSGGFAYVLDADGEFARRCNSAMVDLEPLVEPEDEARVKRLIENHHKYTSSKRAAEI
                     LDKWPQYRARFVKVFPKEYRRALAELAHKKVAA"
BASE COUNT          580 a         1327 c         1282 g          705 t
ORIGIN      
        1 gacgagcgac tgtccgcggc gcatgtcgcc cgcggcgaac accttcggga ccgacgtggc
       61 gtaggcgccg gggccctcgg tcggcgccct ggcgttgccg cgctcgtccc gctgcaaacc
      121 aaactcctcg agcaggctct gcagcggcga cacgaagccc atcgcgagta gcacgagctc
      181 ggccggcatc tcgaaagccg agccggggac ctcgtgcggc ttgccgtcct tccattccag
      241 gcgcaccgcc cgcagcgcct ccactttggc attcttcccc aggaactcct tggtggcgat
      301 ggaccagtcg cggttcacgc cttcctcgtg agacgacgag gtgcgtagcc gcgtaggcca
      361 gtacggccat accgggtttc gctccacatc gggaggcatc ggcagcagct cgaactgggt
      421 gatcgatctc gcgccgtgcc ggttagacgt gccgacgcag tcggatcccg tgtcgccgcc
      481 gccgatgatc acgacatgct tgccggcggc ggagaggtcg cggatcccgg cgtcaccggc
      541 gacgcgcttg ttctgcagcg gcaagaactc catggcgaaa tgcacgccgt cgagcccgcg
      601 gcccgggatc ggcaggtcgc gcggctgctc ggcgccgccg gcgaggacta tggcatcgaa
      661 ctcggcgagc agcttctgcg gcgcgatgtt ctggtgcgcg tcgttcggga tcgcgctccc
      721 cggccgctgc acgccgacga ataccgcggt gcggaacttc acgccttcgg cgcgcagctg
      781 ctccaggcgc cggtcgatga ctgccttctc aagcttgaag tccgggatgc cgtagcgcag
      841 caggcccccg atgcggtcgc tcttctcgaa cactgtgacc gcgtgcccag cgcgcgcgag
      901 ctgctgcgcg cacgcaagcc ctgcgccgcc cgaaccgacg attgccactt gcttgccgct
      961 cttgtgcgcg gccggctgcg gctgcaccca gccttcttcc caggccttgt cgataatcgc
     1021 gtgctcgatc gacttgatgc cgaccgggtc ggcgttgatg cgcagcacgc acgcttcctc
     1081 gcacggcgcg gggcagatgc ggccggtgaa ctcggggaag ttgttggtgg agtgcagcac
     1141 ctcgatcgcg gctttccagt cctggcgata tacgaggtcg ttccagtccg gaatgatgtt
     1201 gttcaccggg cagccggaca tgcagaacgg gatgccgcag tccatgcagc gcgcgccctg
     1261 aatcccggct gcctcgtcgg acagccgcag gtagaactcg cggtaatgct tcttgcgcgc
     1321 ttcgaccggc tcgtgcggct cctcgagccg ctcgtgctcg aggaacccgg tgaccttccc
     1381 cattatgcgg cgaccgtcac gcggccacct tcttgtgcgc gagctccgcc aaggcccggc
     1441 ggtactcctt cgggaatacc ttgacgaagc gcgcccggta ctgcggccac ttgtccagga
     1501 tctcggccgc gcgtttggag ctggtgtact tgtggtgatt ctcgatgagg cgcttgacac
     1561 gcgcctcgtc ctcgggctcg acgagcggct cgaggtcgac catcgcgctg ttgcagcgcc
     1621 tggcgaattc gccgtcggca tcgagcacgt acgcgaagcc tcccgacatg ccggccgcga
     1681 agttccttcc ggtgctgccc aagacgacta cggtgccgcc ggtcatgtat tcgcagccgt
     1741 ggtcgcccac gccctccacg acggcgcgcg cacccgagtt gcgaaccgcg aagcgctccc
     1801 cggcgacgcc gcggaagtac gcctcgcccg cgatcgcgcc gtagagcacc acgttgccgg
     1861 tgatgatgtt ctcggtcggc tcgccgcgga acttgggcga gggctgcacc gagatccggc
     1921 cgccggagag ccctttgccg cagtaatcgt tggtgtcgcc caccagatcg agcgtcacgc
     1981 ccttcgcgag gaaggcgccg aagctctggc cggccgagcc cgcgaggcgg atatggacgg
     2041 tgtcgtcggg cagcccgtcg tgcccgtagc gcttcgcgat ctcgtaggaa agcatcgtgc
     2101 ctacggtgcg attgatgttg cggatcgggg tctcgatcgc gaccttttcg ccgcgctcga
     2161 gcgccggccg cgcgagctcg atcagccgat tgtcgagcgc cttttccagg ccgtggtgct
     2221 gcacctcatc atggaaacgc gacacttcgg gcggcatctt cggcatggcg aagacgcgcg
     2281 agaagtcgag gcccttcgcc ttccagtgct cgatgccgtt ctttgtgtcg aggaattccg
     2341 tgcggccgat caattcattg agccgacgaa cgccgagctg cgccattatt tcgcgcgcct
     2401 cttcggcgac gtggaagaag tagttgacca cgtgctcggg cttgccctcg aacttgcgcc
     2461 gcagcaccgg gtcctgcgtc gctacgccga ccggacacgt gttcagatgg cacttgcgca
     2521 tcatgatgca gccctcgacg acgagcggcg cggtggcgaa gccgaactcg tcggcgccga
     2581 gaatcgcgcc gatcaccacg tcgcggccgg tcttcaactg gccgtcgacc tggaccgcga
     2641 ttcgtcccct cagccgattc agcaccagcg tctgctgcgt ctcggcgagg cccagctccc
     2701 acggcgtgcc cgcatgcttg atcgacgacc acggtgaggc gccagtaccg ccgtcgtggc
     2761 cggcaatcgt cacgtgatcc gacttgccct tggacacccc ggccgcgatg gtgccgacgc
     2821 cgacctcgga tacgagcttc accgaaatcg cggcgttcgg gttggaattc ttcaggtcgt
     2881 ggatgagctg cgccaggtcc tcgatcgaat agatgtcgtg gtgcggcggc ggcgagatga
     2941 gctccacgcc gggggtcgaa tagcgcagct cggcgatgta ctccgagacc ttgcgtccgg
     3001 ggagctgacc gccctcgccg ggcttggcgc cctgcgcgat cttgatctgc agcatctccg
     3061 cgctcgccag gtactcggcg gtgacgccga aacgccccga ggccacctgc ttgatcttcg
     3121 acttgagcga atcgccctcc cgcagcggga tgtcgacctc gacgcggctc ttgcccagcc
     3181 tgccttgcag cgattcgccg gccttcaccg gcgcatagcg cctgcggtcc tcgccgcctt
     3241 cgccggtgtt cgagcgtccg ccgatgcggt tcatggcgat ggcgagcgtg gtgtgcgcct
     3301 cggtcgagat cgagccgagc gacatcgcgc cggtcgagaa gcgcttcacg atctcggcgg
     3361 cgggctcgac ctcctcgatc ggcacgggcg ggcacagccc cgagcggaac tcgaacaggc
     3421 cgcggaaggt catgtgacgg cgcgactggt cattgatgat cgccgcgtat tccttgtacg
     3481 tggcatagga gttggcgcgc gaggcgtgct gcagcttggc gatcgcgtcg ggggtccagg
     3541 tgtggtcctc gccgcgcacg cgccaggcgt actcgccgcc ggcgtcgagc atgctggcaa
     3601 gcaccgggtc gcgctcttcg aacgcggcgc gatggatgcg aatcgcctct tccgcgaggt
     3661 cgaaaaggtc gatgccctcg acgttggacg ccgtgccggt gaagtacttg tcgaccagcg
     3721 actttgccag gcccactgcc tcgaagatct gcgagccggt gtaggacatg taggtcgaga
     3781 tgcccatctt ggacatgacc ttgcgtaagc ccttgccgat cgccttgatg aagttcttgc
     3841 acgccttgcc gccatcgagc gccgcaccga gcttgccgcc ttcggcgagc tcta
//
LOCUS       DASUNT010000461         3926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1204583, whole genome shotgun sequence.
ACCESSION   DASUNT010000461 DASUNT010000000
VERSION     DASUNT010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204583"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="VFX94_09320"
     CDS             <1..140
                     /locus_tag="VFX94_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEX5768419.1"
                     /translation="LFGVMAGGEPGARRALEILSDELQRAMKLCGVRSIAEIGPHLLF
                     R"
     gene            complement(172..1338)
                     /locus_tag="VFX94_09325"
                     /pseudo
     CDS             complement(172..1338)
                     /locus_tag="VFX94_09325"
                     /inference="COORDINATES: protein motif:HMM:NF006021.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
     gene            complement(1335..2141)
                     /locus_tag="VFX94_09330"
     CDS             complement(1335..2141)
                     /locus_tag="VFX94_09330"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5768420.1"
                     /translation="MQEKVFRDYDQKALDDQYEQRVWVAHADEIIKRYAQKSDAVRKR
                     LGEPRVARYGPTPPETLDIYGTGKRAFVFVHGGAWKRQSSREQGFLAEPVLQAGAAYI
                     ALNFANLPAVTLPEMAAQVCRALEWIRKELTDHIVLCGHSSGAHLAACALMKTNLVRK
                     AMVVSGIYDLLPVRLSARNDYVRLDERLEHEYSPIRHVGDIECRVTVGWAEKESAEFL
                     RQSREFAAALENAGKLTCSLEGKSLNHFEILETMADPASPLGRAALNMLQ"
     gene            complement(2098..2358)
                     /locus_tag="VFX94_09335"
     CDS             complement(2098..2358)
                     /locus_tag="VFX94_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEX5768421.1"
                     /translation="MALGVRGPYIGPMVIAAKSASQVLLHMAVAFAVMYAFTGSLAFG
                     GIAAIVEPLCNVLLLPLHDRLWRRIARRRARKGLPRLRPEGT"
     gene            complement(2394..2852)
                     /locus_tag="VFX94_09340"
     CDS             complement(2394..2852)
                     /locus_tag="VFX94_09340"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEX5768422.1"
                     /translation="MNVAQFLYHEARLLDTGQLEAWLDLFTEDATYWVPLERGQKDPY
                     ETSSIIHDDRTLLGLRVKQARHPRAHARQPLARTVHQVGNVMVLSQSDSELAVASTLH
                     LVEFRNARQRHYGALVEHRLRPAGERYRIAHKRVDLVNSEGELEGIAVLF"
     gene            complement(2849..>3926)
                     /locus_tag="VFX94_09345"
     CDS             complement(2849..>3926)
                     /locus_tag="VFX94_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5768423.1"
                     /translation="VVNRCMHRGAMVCAEGRGNTERFVCPYHGWSYDRSGALRAVPFA
                     SGYDKAKLPTGLKAVPRVKSYRGFIVASLARHGDDLQSFLGPARASFDDFVDRAPGGE
                     LEVAGGVFKHGYNGNWKLMLENHLDGAHPAWVHASSVAVARNAPEPGKPGEERYSDIA
                     VRQMRQNGAPDAVWEAIGIWTTPHGHGYMGDYHDDSRLVAGMGNPVFEDYRRNLIERV
                     GEKEADRILRVTLWNTIIYPNCSFMSQFRQLRIVHPVAVDRSVVTTYSLRMKNAPAQM
                     FRDTIAFSNVVNGTGSWVLTDDLEVYERIQKGFASGAVEWAYIGRGHGRDVMEPNGAA
                     RGATGTSEIFIRAQCAAWLRYMSQ"
BASE COUNT          626 a         1302 c         1303 g          695 t
ORIGIN      
        1 cgctgttcgg cgtgatggcg ggcggcgagc cgggagcgcg gcgcgcgctg gagatcctct
       61 ccgacgagct gcagcgcgcg atgaagctgt gcggggtccg ctcgatcgcc gagatcgggc
      121 cgcacctcct ctttcgttga aattacgcac gtaatcggga gcgctgaaca cccactcgag
      181 cgtgtcgtag aaccgctcga ccggccggcc acggtacatg tcgttgcgca ccaggcgctc
      241 gagcccgtcg ggcacatgaa aaatcatgcc gacgagccgg ttcgcggaaa tctgcacgcg
      301 ggtggcacgg accaggcgct ttccctgata gttcttgaat gcggcagcaa aatctccgtc
      361 ggtctcgtcg gcgcagagcc cgaggcacac cgcgtcctcc atcgccatcg cggcgccttg
      421 cgccatgtac tgcagcatga agtgcgccgc gtcgccgagg agagtcacgc gcccacggct
      481 ccaattgttc accggctcac ggtgcagtag catccagcgg cggaactctt tcggcgtgcg
      541 catgagcttg agcggctttt cgcagtagtg cgcgaagaag gggagcacct cgtcgggctg
      601 cgcaagctcg ttgtgcccgc cgctcgtgtg cttgccgatc acggtggcga ccaggttgaa
      661 gagcttccag ccgcgcagcg ggtagtggac gatgtgcgtg ttgtggccgg cccagagcgt
      721 ggccgccggc agccgcaggt ccttcggcac ctcgtcgatc ttcagcaccg cgcggtagca
      781 catgtgcccg gtggcgggcg gcagcggatc gccgacgatc ttttcgcgga tcaccgagcg
      841 cccgccgtcc gcggcgacga gtgccgcgcc ctcgatgggc tctttctcgg tgagaacgcg
      901 cacggaagac ccatcctgct gaaacccggt cacccgcgtg ctggtgcgca gctcgatcag
      961 ctccggcagc gccttgcagc cgtcgagcag cgagccgtgg atatcggcgc ggtgcgtcac
     1021 cgcgtacgga ttgccgaagc gcttggcaaa gcgcttgtcg agcgggatgt cgattacctt
     1081 ctcgccgctc accccgtcga acatgagcag gtgctcgatg aacaccgcct ccttcttcac
     1141 cagagcgcct acgccgagcg cgtccagggc gtgccacgcg ttgggcgcga tctggatgcc
     1201 ggcgccgatc tcgccgaact gggaggcctg ctcgagcaca accgagcgga accctttgcg
     1261 cgcgagcgcg agcgccgagg cgaggccgcc gatgccgccg ccggcgacga tgacgggggc
     1321 ggcgttgcgg gacctcattg cagcatgtta agcgctgccc ggccgagcgg ggaggccggg
     1381 tcggccatcg tttcgaggat ttcgaagtgg ttgagggatt tgccttccag cgagcaggtc
     1441 agcttgccgg cgttctccag cgcggcggcg aattcccgcg attggcggag gaattcagcc
     1501 gactccttct cggcccagcc gaccgttacg cggcattcga tgtcgcccac gtggcggatc
     1561 ggcgagtact cgtgctccag gcgctcgtcc agccgcacat agtcgttgcg ggcactaagg
     1621 cgtaccggca gcaggtcata gatgccggag acaaccatgg cttttcggac gaggttcgtc
     1681 ttcatcaggg cgcatgccgc caggtgggct cccgacgaat ggccgcacag gacgatgtga
     1741 tcggtgagct cctttcttat ccactccagc gcacggcaaa cctgggcggc catttcgggc
     1801 agcgtcacgg cgggcagatt ggcgaagttg agcgcgatgt aagcggcgcc ggcctggagg
     1861 accggctcgg caaggaatcc ctgctcgcgg ctcgactggc gcttccaggc tccaccgtgc
     1921 acgaagacga atgccctctt tcccgtgccg tagatgtcga gcgtttcggg cggcgtcggc
     1981 ccatagcgcg ccactcgcgg ctcgcccagc cgcttcctta cggcgtcgct cttctgggca
     2041 tagcgcttga tgatctcgtc ggcgtgcgcg acccagacgc gctgctcgta ctggtcgtca
     2101 agtgccttct ggtcgtagtc gcggaagacc ttttcttgca cggcgccgcg ctatccgacg
     2161 ccagagccgg tcgtgcagcg ggagcaacaa tacgttgcaa agcggctcca cgatcgccgc
     2221 gatgccgcca aacgccagcg agccggtgaa ggcgtacatg accgcgaagg cgaccgccat
     2281 gtgcaggagc acctggctcg cgcttttggc tgcaatgacc atggggccaa tataggggcc
     2341 gcgcacgccg agcgccaatt ggatgtcgca atgtcagcct tcaatgccgt ctatcagaaa
     2401 aggaccgcga tgccttcgag ctcgccttcg gagttgacca ggtcgacgcg cttgtgcgcg
     2461 atgcgatagc gctcgccggc gggacgcagg cggtgctcca ccagcgcgcc gtagtgcctt
     2521 tgccttgcgt tcctgaattc caccaggtgc agcgtcgaag ctacggcgag ctccgagtcg
     2581 ctctgcgaga ggaccatcac gttaccgacc tggtgcaccg tgcgcgcgag cggctgccgc
     2641 gcatgcgcgc ggggatggcg cgcctgcttc acgcgcaggc cgagcagcgt gcggtcgtcg
     2701 tgaatgatgg aggaggtctc gtagggatcc ttctggccgc gctcgagcgg gacccagtag
     2761 gtcgcgtcct cggtgaaaag atcgagccat gcctcgagct gcccggtgtc gagcagcctc
     2821 gcttcgtgat agaggaattg cgcgacattc attgcgacat gtagcgcagc cacgccgcgc
     2881 actgtgcgcg gatgaatatc tcgctcgtgc cggtggcgcc gcgcgccgcg ccgttcggtt
     2941 ccattacgtc ccggccgtgg ccgcggccga tgtacgccca ctcgaccgcg cccgaggcga
     3001 agcccttctg gatgcgctcg tagacctcca ggtcgtcggt cagcacccac gagccggtgc
     3061 cgttcacgac gttggagaac gcgatcgtgt cgcggaacat ctgcgccggc gcgttcttca
     3121 tgcgcaacga gtacgtcgtg accacgcttc ggtccaccgc gacgggatgc acgatgcgca
     3181 gctgccggaa ctggctcatg aacgagcagt tcggatagat gatcgtgttc cagagcgtga
     3241 cgcggaggat ccggtcggcc tccttttcgc ccacacgctc gataaggttc cttcgatagt
     3301 cttcaaagac cgggttcccc atgccggcca ccaggcggct gtcgtcgtgg tagtcgccca
     3361 tgtagccgtg gccgtgcggc gtggtccata tgccgatcgc ttcccatacc gcgtccggcg
     3421 cgccgttctg gcgcatctgc cgcaccgcga tgtcggagta gcgctcttcg cccggcttgc
     3481 ccggctcggg cgcgttgcgc gccaccgcga ccgacgaggc gtgcacccac gccgggtgcg
     3541 cgccgtcgag gtggttctcg agcatcagct tccagttgcc gttgtagccg tgcttgaaca
     3601 ccccgcccgc gacttccagc tcgccgcccg gcgcgcggtc gacgaagtcg tcgaacgacg
     3661 cccgcgcggg cccgaggaag ctctggaggt catcgccgtg gcgagcgagc gaggcgacga
     3721 tgaatccccg atagctcttc acgcggggca ccgccttcag cccggtcggc agctttgcct
     3781 tgtcgtagcc gctcgcgaac ggcacagccc tcagcgcgcc ggaacggtca tagctccagc
     3841 cgtggtaagg acacacgaag cgctcggtgt tcccgcgtcc ttctgcgcac accatcgcgc
     3901 cccggtgcat acagcgattt acaaca
//
LOCUS       DASUNT010000462         3265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1116566, whole genome shotgun sequence.
ACCESSION   DASUNT010000462 DASUNT010000000
VERSION     DASUNT010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116566"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1396)
                     /locus_tag="VFX94_09350"
     CDS             complement(<1..1396)
                     /locus_tag="VFX94_09350"
                     /inference="COORDINATES: protein motif:HMM:NF006124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX5768424.1"
                     /translation="MLDPKRSDLVAGVAGAGTMGRGIAQVLAQCGARTLVYDAQPGAA
                     RRGKDSIAQSLVRLVEKGRLRAAEADNALGRIEIVESPSAFQPCHVVVEAIAEDLDAK
                     RKLFSSLEKHVSGECILASNTSSLSVTAMAAACQRPGRVAGYHFFNPVPVMKIVEVVD
                     AVLTEAWVTEALAGLAQRFGHTPVRCKDTPGFIVNHAGRAFVPEALRVLSEGVADFAT
                     IDRILVDAAGFRLGPFALMDLVGLDVSHAVMKSMYEQYFEEPKYRPSFLAEPRVAAGL
                     LGRKTARGWYAYDRDGAAPRTSEPPAPSLRPASVWAVPELKELLAQLGTKPELKPTPD
                     ALCFVAPLGADATTCALELGLDPQRTVAVDPLFGFAKRRTLMRTPVTRKGMLEAAHGL
                     LAADGVPVSLINDSPGFVAQRVVATIVNIGCDIAQQRVASPEDIDCAVTLGLGYPKGP
                     LAMGDALGAATVLTI"
     gene            1453..2397
                     /locus_tag="VFX94_09355"
     CDS             1453..2397
                     /locus_tag="VFX94_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5768425.1"
                     /translation="MRRILTLAGGLLLAAAVQAQQFPSKPVTLIVPWPAGGSTDLYFR
                     KLGEITARHLGQPLVIENRPGGSGMNGPATMAKTAKPDGYTISQLAISAFRMPHMQQV
                     DWDPIRDFTYIIGLAGYTFGIVVKADSPFKTFQDLIAYARANPGRLSYATPGTGTSLH
                     LAMEEIAAKAGVQFLHVPFKGYADGAIALMGGHVMVQVDSTGWAKQVDAGAQRLLATL
                     GDKRTRWNAPTVKELGVDTVSNSPFGLVGPKGMARDVVKVLHDAFKKSLDDPDYLKML
                     AQLDQPAWYMSSEDYARWAVDMLKAERATIERAGLLLK"
     gene            2410..2850
                     /locus_tag="VFX94_09360"
     CDS             2410..2850
                     /locus_tag="VFX94_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5768426.1"
                     /translation="MKYRKILLAYNGSQEGKRALLECADLAGFLQAETHLLAVASMPP
                     SLFLTEGFVPEELLEEEKKRTQTILDEGIRTLRDRGFNATGQLAVGEPVEEICRLART
                     LGVDLIVIGHNQNTSFAARWWKGSVGASLLDYAPCSILIAMSKA"
     gene            2906..>3265
                     /locus_tag="VFX94_09365"
     CDS             2906..>3265
                     /locus_tag="VFX94_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5768427.1"
                     /translation="MLKGRLEDDRMLTGRGRYVSDWNLPGQAYAQFVRSDRAHAEILA
                     IGKDEALGAPGVIGVFTGDDLDLKSLPAALPAKGRGGMELINPGRPALAKGRVRFTGD
                     AVALVVAESPAAAQDAAE"
BASE COUNT          559 a         1057 c         1119 g          530 t
ORIGIN      
        1 ggatcgtcag cacggtcgcc gcgccgagag cgtcgcccat cgcgagcggg cctttcggat
       61 agccgaggcc gagcgtgacc gcgcagtcga tgtcctcggg cgacgcgacg cgctgctgcg
      121 cgatgtcgca gccgatgttg acgatcgtcg cgacgacgcg ctgggcgacg aagccgggcg
      181 agtcgttgat gagtgagacc ggcacgccgt cggcggcgag tagcccgtgc gccgcttcga
      241 gcattccttt tcgggtgacg ggcgtgcgca tcaaggtgcg gcgcttcgcg aacccgaaaa
      301 gcgggtcgac cgccacggtg cgctgcggat cgagacccag ctcgagcgcg catgtcgtcg
      361 cgtcggcgcc gagcggcgca acgaagcaca aggcgtcggg cgtgggcttg agctcgggct
      421 tcgtgccgag ctgtgccagc aattccttga gctccggcac ggcccatacg gaggccggcc
      481 gcaaggaggg ggctggaggc tcggaggtgc ggggcgccgc accgtccctg tcgtacgcgt
      541 accagcctcg cgcggttttt ctgccgagta gtcccgcggc gacccggggc tcggcgagga
      601 aggacggccg gtacttgggc tcttcgaagt attgctcgta catcgacttc atcaccgcgt
      661 gcgagacgtc gaggcccacg agatccatga gcgcgaaggg cccgagccgg aacccggccg
      721 cgtcgacgag aatgcggtcg atggtggcga agtcggcgac gccctccgac agtacgcgca
      781 gcgcttcggg cacgaatgcc cgccccgcgt ggttcacgat gaacccgggc gtgtccttgc
      841 agcgcaccgg cgtgtggccg aagcgttgcg cgaggccggc gagcgcttcg gtgacccacg
      901 cctcggtgag gactgcgtcg accacctcga cgatcttcat taccggcacc gggttgaaga
      961 agtggtagcc ggcgactcgg ccgggacgct ggcacgccgc cgccatcgcg gtcacggaca
     1021 gcgaggaagt attggaggca agaatgcatt cgccgctcac gtgcttttcg aggctcgaga
     1081 acagctttcg ctttgcgtcc aggtcctcgg cgatcgcttc aaccacgacg tggcagggct
     1141 ggaaggcgct tggggactcg acgatttcga tccggccgag cgcattgtcg gcctcggcgg
     1201 cgcgcagccg gcctttttcg accagcctga cgagcgattg cgcgatggag tccttccccc
     1261 tgcgggccgc gccgggctgg gcgtcgtaga cgagcgtccg cgcgccgcac tgggcaagaa
     1321 cctgcgcgat gccgcggccc atagtccccg caccggccac gccggcgacg agatcgctgc
     1381 gcttgggatc tagcaagccg ttattttagc ggcgcaggcg gcggctataa tcgccgagag
     1441 gaggaagcaa gggtgagaag gatactgacg ctggcgggag ggctgctcct tgcggccgcg
     1501 gtgcaggcgc agcagttccc gtcgaagccg gtcacgctca tcgtgccctg gccggccgga
     1561 ggcagcaccg acctttattt ccgcaagctg ggcgagatca ccgcccggca tctcggccag
     1621 ccgctggtga tagaaaaccg tcccggtggc agcggcatga acggccctgc caccatggca
     1681 aagacggcga agccagacgg ctacaccatc tcgcagcttg ccatctcggc tttccgcatg
     1741 ccgcacatgc aacaggtcga ctgggacccg attcgcgatt ttacctacat catcggtctt
     1801 gccggctata cgttcggcat cgtggtgaag gccgactcgc ctttcaagac attccaggat
     1861 ctcattgcct acgcgcgtgc gaaccccggc aggctttcct atgccacgcc ggggacgggc
     1921 acttcgctcc atctcgccat ggaggagatc gcggcgaaag cgggcgtgca gttcctccac
     1981 gtgccgttca agggctatgc cgacggcgca atcgcgctga tgggcggcca cgtcatggtg
     2041 caggtcgatt ccacgggctg ggcgaagcag gtggacgccg gcgctcagcg cctgctcgcg
     2101 acgctcggcg acaagcgcac gcgctggaac gcccccacgg tgaaggagct cggcgtggac
     2161 accgtttcca actcgccctt cggcctcgtg ggccccaagg gaatggcgcg cgacgtggtg
     2221 aaggtgctgc acgatgcatt caagaagtcg ctggacgatc ccgattacct gaagatgctg
     2281 gcgcagctcg atcagccggc ctggtacatg tcgagcgagg attacgcccg ctgggccgtc
     2341 gatatgctca aggccgagcg cgccaccatc gagcgcgcgg ggttgctact caagtaaagg
     2401 agggtggcca tgaagtatcg caagattctt ctcgcctaca acgggtcgca ggaaggcaag
     2461 cgcgcgctgc tcgagtgcgc agacctggcg ggcttcctcc aggccgagac gcacctgctc
     2521 gccgtggcga gcatgccacc gagccttttt ctcaccgagg gcttcgtgcc cgaggagctg
     2581 ctcgaggaag agaagaagcg cacccagacg atcctcgacg aaggcatccg tacgctgcgc
     2641 gaccgcggct tcaacgcgac ggggcaactc gccgtaggcg agccggtcga ggagatctgc
     2701 cggctcgcgc gcacgctcgg cgtagacctg atcgtcatcg gccacaacca gaacacctcg
     2761 ttcgcggcgc gctggtggaa gggctcggtg ggagcatcgc tgctcgacta cgcgccctgc
     2821 agcatcctca tcgcgatgtc gaaagcatag attcgggccg cgtcgccgaa ttggttcggg
     2881 ctgcgtcccc gaatacaatt cgggcatgct gaaaggcagg ctcgaagatg accgaatgct
     2941 caccggtcgg ggacgctatg tctccgactg gaatctcccc ggccaggcgt acgcgcagtt
     3001 cgtgcgctcg gaccgcgccc atgcagaaat tctcgcgatc ggaaaagatg aggcgctagg
     3061 agcaccgggc gtcatcggag tctttaccgg cgacgatctc gatctcaagt ccctgcctgc
     3121 cgcgcttccc gccaaaggcc gcggcggcat ggagctcatc aatcccggaa ggcccgcgct
     3181 cgccaagggt cgcgtgcgct tcaccggcga cgcggtggcg ctcgtcgttg ccgagagtcc
     3241 ggctgccgcg caggatgcgg ccgag
//
LOCUS       DASUNT010000463         2679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_765816, whole genome shotgun sequence.
ACCESSION   DASUNT010000463 DASUNT010000000
VERSION     DASUNT010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765816"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="VFX94_09370"
     CDS             <1..1192
                     /locus_tag="VFX94_09370"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5768428.1"
                     /translation="FEFFAMRDPTINAFAMPGGFVGVHTGLLLAAESESEVASVLAHE
                     IAHVTQRHIARMLGQQQQMQLPLLAAMAAAILLGGSRPDLAAGAITATQAGAVQTQLA
                     YSRDFEREADRIGFQALEGAGFDVNAMGAFFEKMQRSQRVADDGSLPGYLRTHPVTTE
                     RIADAQNRAASRSYKQHLDSPEFHLVRAKLRAGSGDARDTVEFFQSSVREKRYASELA
                     ARYGLAASLLRAQRAKEAQAELDRARAAGAAGPMVETLAARIRLALGDRAGAAALLGD
                     AHTRFPHSRPLLYAHAEALQEAGRNERAQGLLTDALRAHPRDPRLHQLQAKNYAALGK
                     RLLQHQSQAEVYALHGSLPAAIEQLQIARAAGDGDFYQLSVVDARLKELRAQHAQELK
                     DQKR"
     gene            complement(1189..2151)
                     /locus_tag="VFX94_09375"
     CDS             complement(1189..2151)
                     /locus_tag="VFX94_09375"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEX5768429.1"
                     /translation="MEEREKRPAAPSAWLATITQLPTEDPAARMQVLRTLESLGAAVM
                     REGVYLLPDTPANRQSLEALADYIGKIGGSANMLHVQAASAAQQELFERLFDRSARYE
                     ALIKTVESLRVGYGTSDPSAISRVVHKQRREFESIAALDFFPNAARRRAEQALAIAEA
                     EVRRLLFPAQAQPALAPGALLGRTWVTRKPLWADRLACSWLIRRFVDPQATLTWLEKS
                     EALPGDSIGFGFEGAHFGNSQTRVTFEEMLLKLDLGRNPALAKIGSIVHFLEIRGTPV
                     AEAAGVQTLLQGAQRRSNSEKELLAEAEKTFDLLYEAYCPAPKN"
     gene            2169..2660
                     /locus_tag="VFX94_09380"
     CDS             2169..2660
                     /locus_tag="VFX94_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX5768430.1"
                     /translation="MQRALLALVGLAAAFGARAADGTFSVRMLTPETALKASQAALRS
                     CRDRGFQVAVAVVDRMGVVQALLRDRYAGPHTPDMASAKAYTAVSFRTNTTELAEASQ
                     PGRPASGIRHRPRIAAVGGGMMIEAGGSLLGAIGVSGAPGAREDDACAADGIAAIRED
                     IEL"
BASE COUNT          394 a          954 c          939 g          392 t
ORIGIN      
        1 cttcgagttc ttcgcgatgc gcgacccgac catcaacgcg ttcgcgatgc cgggcgggtt
       61 cgtcggcgtg cacaccgggc tgctgctcgc ggccgagagc gaatccgagg tggcctccgt
      121 gctggcgcac gaaatcgcgc acgtcaccca gcggcacatc gcgcgcatgc tcggccagca
      181 gcagcagatg cagctgccgc tgctcgcggc gatggcggcg gccatcctgc tcggcggctc
      241 gcgccccgac ctcgccgcgg gcgccatcac ggcgacccag gcgggcgcgg tgcagaccca
      301 gctcgcctac tcgcgagact tcgagcgcga ggccgaccgc atcggcttcc aggcgctcga
      361 gggtgcgggc ttcgacgtga acgcgatggg cgccttcttc gagaagatgc agcgcagcca
      421 gcgggtggcc gacgacggct cgctgccggg gtacctgcgc acccacccgg tcaccacgga
      481 gcgcatcgcc gacgcgcaga accgcgccgc cagccgctcg tacaagcagc acctcgacag
      541 cccggagttc cacctggtac gcgcgaagct gcgcgccggc tcgggggatg cgcgcgacac
      601 ggtggaattc ttccaaagct cggtacgcga gaagcgctat gcctccgagc tcgcggcgcg
      661 ctacggcctc gctgcctcgc tgctgcgcgc gcagcgggcc aaagaggcgc aggcggagct
      721 cgaccgcgcc cgcgcggcgg gcgccgccgg accgatggtc gagacgctcg cggcgcgcat
      781 ccgcctcgcg ctcggcgatc gcgccggcgc ggcggcgttg ctcggcgacg cgcacacgcg
      841 ctttccgcat tcgcgcccgc tgctctacgc gcacgccgaa gcgctgcagg aagccggccg
      901 caacgagcgg gcgcaggggc tgctcaccga cgcgctgcgg gcgcatccgc gcgatccgcg
      961 cctgcatcag ctccaggcca aaaactacgc tgcgttgggc aagcgcctgc tgcagcacca
     1021 gtcgcaggcg gaggtctatg cgctgcacgg gagcctgccc gcggcgatcg agcagctgca
     1081 gatcgcgcgc gccgccggcg acggcgactt ctaccagctc tccgtcgtcg atgcgcgcct
     1141 caaggagctt cgcgcgcagc acgcgcagga gctcaaggac cagaagcgct agttcttcgg
     1201 cgccgggcaa tacgcttcgt agagcagatc gaacgttttt tcggcttctg cgagcagctc
     1261 tttctcgctg ttcgagcggc gttgcgcgcc ctgcagcagc gtttgcacgc cggccgcctc
     1321 ggcgaccggc gtgccacgga tctcgaggaa gtgcacgatg ctgccgatct tggcgagcgc
     1381 gggattcctg ccgaggtcga gcttgagcag catctcctcg aaggtgacgc gcgtctggct
     1441 gttgccgaag tgcgcgcctt cgaagccgaa gccgatgctg tccccgggca gcgcctcgct
     1501 cttctcgagc caggtgagcg tcgcctgcgg gtcgacgaag cgccggatga gccacgagca
     1561 ggcgaggcgg tcggcccaca gcggcttgcg cgtcacccag gtgcgcccga gcagcgctcc
     1621 gggcgcgagc gcgggttgcg cctgcgccgg aaagagaagc ctgcgcacct cggcttcggc
     1681 gatcgcgagc gcctgctccg cgcgcctgcg cgctgcgttc gggaagaagt cgagcgccgc
     1741 gatcgactcg aattcgcgcc gctgcttgtg caccacccgc gaaatcgcgc tcgggtcgga
     1801 tgtgccgtag cccacgcgca ggctctccac cgtcttgatc aaggcctcgt agcgcgccga
     1861 gcggtcgaac aggcgctcaa acagctcctg ctgcgcggcc gaggccgctt gcacatgcag
     1921 catgttcgcc gaaccgccga tcttgccgat gtagtcagcg agggcttcga gcgattgccg
     1981 gttagccggt gtgtcgggca gcaggtaaac gccttcgcgc atcaccgccg cgccgagcga
     2041 ctccagcgtg cgcagcacct gcatgcgggc ggccgggtcc tcggtgggca attgggtaat
     2101 cgtcgcgagc catgccgacg gcgctgccgg ccgcttctcc ctttcctcca tgcggcataa
     2161 taactttcat gcagcgtgcg cttctcgcgc ttgtcggtct tgcggctgcg ttcggcgcgc
     2221 gcgcggccga cggcacgttc agcgtcagga tgctcacacc ggaaaccgcg ctcaaggctt
     2281 cgcaggcggc gctcaggagc tgccgcgatc gcggcttcca ggtcgcggtc gctgtggtgg
     2341 atcgcatggg cgtggtgcag gcgctgctgc gcgaccgcta cgccggcccg cacacgccgg
     2401 acatggcgag cgccaaggcc tataccgcgg tgagcttccg taccaacacc acggagctgg
     2461 ccgaagcgtc gcagcccggc cggccggcga gcggcatccg ccaccgaccc cgcatcgccg
     2521 cggtgggcgg cgggatgatg atcgaggcgg gcggctcgct tctcggcgcg atcggggtct
     2581 cgggcgcgcc gggcgcgcgc gaggacgacg cctgcgccgc ggacggcatc gcagcgattc
     2641 gcgaagacat cgagctgtga aacaatccgg cgcatgaaa
//
LOCUS       DASUNT010000464         3075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_64598, whole genome shotgun sequence.
ACCESSION   DASUNT010000464 DASUNT010000000
VERSION     DASUNT010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64598"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1096)
                     /locus_tag="VFX94_09385"
     CDS             complement(119..1096)
                     /locus_tag="VFX94_09385"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX5768431.1"
                     /translation="MTRIAAALAALLFTFAVSAQEFTLRAGHDQPVGSMYDEGHQMFK
                     KLVEERSKGRVRVNIFPAAQLGAEVAMVEGLRLGSIDVICANAPNAAAFVPELGLFSV
                     AYLFKDIAHFERVVNDPAFAKRVDEIIAAKNIGIRRIGFYAAGVRNIYSRKGSVGGPE
                     DLKGVKIRVQNNPVEVKVWRAFGAIPTPMNFGEVYQALQSGVLDAAENGLAVIESNKH
                     YEAAKFITQSEHQRNLSALYINEKKLASMPADLQKIVLDAASEAAVHERKRDAELVAA
                     AANSLKTKGAVLTVPDKSKFIALVAPIQDEVARDLKASDLLQIIRKHAQ"
     gene            1166..1891
                     /locus_tag="VFX94_09390"
     CDS             1166..1891
                     /locus_tag="VFX94_09390"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX5768432.1"
                     /translation="MIFNGLKPRKRNLFGQAVEELGSRIVCGELQPGQLLLESDVCRE
                     LGTSRTVVREALKSLSAKGLVDTRTRTGTRVLEATHWNLLDLDVLGWRYAAMPRIQFF
                     RELFELRRMIEPSAAALAAERATKADIEAIARAYADMQSAPHESAAAIDADLRFHRAL
                     LAAAHNELLAQMAGVIGAGLLTSFRISSSSFDVFVPQHGEVLEAIRYGRAPAARAAME
                     KLLVSTREFLERELEEPASAATQ"
     gene            complement(1830..3053)
                     /locus_tag="VFX94_09395"
     CDS             complement(1830..3053)
                     /locus_tag="VFX94_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5768433.1"
                     /translation="MKSEPESGYAWLRLCASLALMTVGGVGMYAIPVALPPVQAGFQA
                     GRGDASLPYTLTMIGFGIGGILMGKAADRFGVVVPVIVGAFALGSGFALAGSAPNLLT
                     FSLVHGLLIGLLGCSATFAPLLADTSLWFNRRRGIAVAICASGNYLAGALWPPVLQYS
                     FDTMGWRATYLGIGVFCTASILLLAFALRRRPPALEAATQIPMRVFGDRPLGLSANAL
                     MALLVVAGLACCVAMSMPQVHIVAYCGDLGYGAARGAEMLSLMFGFGVASRVAFGFIT
                     DRLGGLRTLLLASILQAIAIALFLPFDSLVSLYVVSALFGIFQGGLVPQYAIVVREYF
                     PPREAGVRVGIVLMSTLLGMALGGWMSGAIFDLTGSYRAAFFNGFAWNVLNIAIIAWL
                     LMRALPARARETPAY"
BASE COUNT          568 a          995 c         1044 g          468 t
ORIGIN      
        1 acacggcgaa gacatagcgc agcacgacct ggatgaacac cccgatcgtg atgaacgcca
       61 gcagcccggc gagcgccggc tcgagcacgc gctcgagccg ggcgaagaag cgatccacct
      121 actgcgcgtg cttgcggatg atctgcagca ggtcgctcgc cttgaggtcg cgggccacct
      181 catcctggat cggcgcgacc agagcgatga actttgattt gtcgggcacc gtgagcaccg
      241 cgcccttcgt cttgaggcta ttcgccgccg ccgcgacgag ctcggcgtcg cgcttacgct
      301 cgtgcacggc ggcttcgctc gccgcgtcga gcacgatctt ctgcaggtcg gccggcatcg
      361 aggcgagctt cttctcgttg atgtaaagcg cggacaggtt gcgctggtgc tccgactggg
      421 tgatgaactt cgccgcttcg tagtgcttgt tcgactcgat caccgcgagc ccgttctcgg
      481 cagcgtcgag cacgcccgac tgcagcgcct ggtagacctc gccgaagttc atcggcgtcg
      541 gaatcgcgcc gaaggcgcgc cacaccttca cttcgaccgg gttgttctgc acgcggatct
      601 tcacgccctt caggtcctcc ggcccgccga ccgagccttt gcggctgtag atgttgcgca
      661 cgccggcggc gtagaagccg atgcgcctga tgccgatgtt cttggccgcg atgatctcgt
      721 ccacgcgctt ggcgaacgcg gggtcgttga ccacgcgctc gaagtgcgcg atgtccttga
      781 agagatacgc cacgctgaag aggcccagct ccgggacgaa ggcggcggcg ttaggcgcgt
      841 tggcgcagat gacgtcgatc gagcccaggc gcaggccctc gaccatggcg acttcagcgc
      901 cgagttgggc cgccgggaag atattgaccc gcacgcggcc cttcgagcgc tcctcgacca
      961 gcttcttgaa catctggtgg ccttcgtcgt acatgctgcc gaccggctgg tcgtggccgg
     1021 cgcgcagggt gaactcctgg gcagaaactg cgaacgtgaa caacagcgcc gcgagcgcgg
     1081 cggcaatccg ggtcatggcg tcctcctctt gaacggtacc cctgcaattt aatttatcat
     1141 atgaaatgag gaatgtggct accacatgat tttcaacggc ttaaagccta gaaagcgcaa
     1201 cctgttcggc caggcggtgg aggagctggg gagccgcatc gtgtgcggcg agctacagcc
     1261 cgggcagctg ctgcttgaat cggatgtgtg ccgcgagctc ggaacgagcc gcaccgtggt
     1321 gcgcgaagcc ctcaagtccc tgtcggcgaa aggactggtc gacacgcgca cccgtaccgg
     1381 cacccgcgtg ctggaagcca cgcattggaa cctgctcgat ctggatgtgc tcggctggcg
     1441 ctacgctgcc atgccgcgca tccagttctt tcgcgagctg ttcgagctac ggcgcatgat
     1501 cgagcccagt gcggcggcgc ttgcggcgga acgggcaaca aaggcggata tcgaagccat
     1561 cgcgcgggcg tacgcggaca tgcagagcgc gccgcacgaa agcgcggccg cgatcgacgc
     1621 cgacctgcgt ttccaccgcg cgctgctcgc cgccgcgcac aacgagctgc tcgcgcagat
     1681 ggccggggtc attggcgccg ggctgctcac cagctttcgc atctcgtcca gctcattcga
     1741 cgtgttcgtg ccgcagcacg gagaggtgct ggaggcgatc cgctacgggc gggcgccagc
     1801 ggcgcgcgcc gcgatggaga aactgctggt cagtacgcgg gagtttctcg agcgcgagct
     1861 ggaagagccc gcatcagcag ccacgcaatg atggcgatgt tgagcacatt ccaggcgaag
     1921 ccgttgaaga acgccgcgcg gtaggagccg gtcaggtcga agatcgcgcc cgacatccat
     1981 ccgccgagcg ccatgccgag cagggtcgac atcagcacga tgccgacccg cacgccggcc
     2041 tcgcgcggcg ggaagtactc gcgtaccacg atggcgtact ggggcacgag cccgccctgg
     2101 aagattccga acagcgctga aacgacgtag agcgatacga gcgagtcgaa aggcaggaag
     2161 agcgcaatcg cgatcgcctg caggatcgag gcgaggagca gcgtgcgcag cccgccaagc
     2221 cggtcggtga tgaagccgaa ggcgacccgg ctcgcgacgc cgaagccgaa catgagcgat
     2281 agcatctccg cgccgcgcgc cgcgccgtag ccgagatcgc cgcagtaggc gacgatgtgg
     2341 acctgcggca tcgacatcgc gacgcagcag gccaggccgg ctaccacgag caatgccatc
     2401 agggcgttgg ccgacaagcc cagcggcctg tcgccgaaga cgcgcatcgg aatttgcgtt
     2461 gcagcttcca gcgcgggcgg ccggcgccgc agcgcgaagg cgagcaggag gatcgaggcg
     2521 gtgcagaaga cgccgattcc gaggtaggtc gcgcgccagc ccatcgtgtc gaaggagtac
     2581 tggaggaccg gcggccagag ggcgccggca aggtagttgc cgctggcgca gatcgcgacc
     2641 gcgatgccac ggcggcggtt gaaccagagg gacgtatcgg cgaggagcgg cgcgaaagtc
     2701 gcggagcagc cgagcagccc gatgaggagg ccgtgcacca ggctgaaagt cagcaggttc
     2761 ggcgccgagc cggcgagcgc gaagccgctg cccagcgcaa atgcgcccac gatcaccggc
     2821 accactacgc cgaaacggtc cgcagccttg cccatcagga tgccgccgat gccgaagccg
     2881 atcatcgtca gcgtataggg aagcgaggca tcgcctctgc ccgcttggaa ccccgcctgc
     2941 accggcggaa gcgccaccgg aatcgcatac atgccgacgc cgccgaccgt catcagcgcg
     3001 agcgaggcgc agaggcgcag ccaggcgtag cctgattcgg gttcgctctt caccggttca
     3061 tagtatcgtc gggct
//
LOCUS       DASUNT010000465         2046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_590230, whole genome shotgun sequence.
ACCESSION   DASUNT010000465 DASUNT010000000
VERSION     DASUNT010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590230"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="VFX94_09400"
     CDS             complement(<1..168)
                     /locus_tag="VFX94_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5768434.1"
                     /translation="MSVKSLLDLSGRVALITGGSRGLGLQIAEALAEMGAEVAITARK
                     KDELEQAVKHLG"
     gene            201..824
                     /locus_tag="VFX94_09405"
     CDS             201..824
                     /locus_tag="VFX94_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768435.1"
                     /translation="MTTLVMFSGGIDSTAMLVKLLTEGTDELRVHHIHMQNREGRDAA
                     EQRAVDSILSWCTEHYRPFRYSESGLDFRALEAIPIDYLSIAFVACQVAIDTPRCSRI
                     AVGALAADTDIENRSARQRRVFDAMYECYRARKLGEPRVEWIYPVYSLPKAEIAAALP
                     AGLLDLTWSCRRPVGGSRPCLVCKACKARQFAQGLRTPAEAAQNQIS"
     gene            841..>2046
                     /locus_tag="VFX94_09410"
     CDS             841..>2046
                     /locus_tag="VFX94_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768436.1"
                     /translation="MRTRRGDGRILIYSHDTFGLGHLRRCRTIAHALVERFKQLSVLI
                     LSGSPIIGSFDFKARVDFVRVPGVVKLRNGEYTPLNLHLDIRETMAMRASIIQHTAEA
                     FGPDMLIVDKEPVGLRGELTQTLKMLKRDGTRLVLGLRDVMDEPRLLEPEWQRKKAIP
                     ALRDLYDEIWVYGLPQICDPLEGIALPARVRQKMVYTGYLHREVPTHGAPPRLPEMCN
                     RPFLLVTTGGGGDGDGLIDWVLRAYEHDPLLPYPALLVLGPFMQSERQAEFMDRAAKL
                     KRVDAITFHGQLEALVDRAAGVVAMGGYNTFCEVLSLDKRALIVPRTEPRLEQLIRAS
                     RAAELGLVSMLSDDGTYDPAVMAAALRALPRQNLPSEVVVPGLLEGLKNVSRLVAPSI
                     EPREVEVARR"
BASE COUNT          320 a          722 c          684 g          320 t
ORIGIN      
        1 gcccaggtgt tttaccgcct gttccagctc gtccttcttg cgcgccgtga tggcgacctc
       61 ggcccccatc tccgcgagcg cctccgcaat ctgcaggccg agcccgcgcg agccgccggt
      121 gatgagcgcc acgcgcccgg agagatccag cagggatttc acgctcatgg gccgcatcgt
      181 agtacaagta gaatcgaggc gtgaccaccc tagtcatgtt ttccggcggc atcgacagca
      241 cggcgatgct ggtgaagctg ctcaccgaag gcacggacga gctgcgcgtg caccacatcc
      301 acatgcagaa tcgcgagggc agggatgcgg ccgagcagcg tgcggtggat tcaattctct
      361 cctggtgcac ggagcattac cgccccttcc gctactccga atccggcctc gacttccgcg
      421 cgctggaagc gatcccgatc gattatctgt cgatcgcctt cgtcgcctgc caggtggcga
      481 tcgatacgcc gcgctgcagc cgcatcgcgg tcggcgcgct cgccgcggac accgacatcg
      541 agaaccgcag cgcgcgccag cgccgggtgt tcgacgcgat gtacgagtgc taccgcgcgc
      601 gcaagctggg cgagccgcgc gtcgagtgga tttaccccgt ctacagcctg ccgaaggccg
      661 agattgctgc agcgctccca gccggcctgc tcgacctgac ctggtcctgc cggcgtcccg
      721 tgggcggttc gcgcccctgc ctcgtctgca aagcatgcaa agcgaggcag ttcgcacagg
      781 gcttgcgtac ccccgccgag gcagcccaaa atcagatctc ctaagggggg agggggcgtc
      841 atgaggacca ggcggggcga cggccgcata ctcatctata gccacgacac cttcggcctc
      901 ggccacctgc gccgctgccg cacgatcgcg catgcgctgg tcgagcgctt caagcagctc
      961 tcggtcctga tcctgtcggg ctcgccgatc atcggcagct tcgatttcaa ggcgcgcgtc
     1021 gacttcgtgc gcgtgcccgg cgtggtcaag ctgcgcaacg gcgagtacac gccgctcaac
     1081 ctgcacctcg acatccgcga gacgatggcg atgcgcgcct cgatcatcca gcacaccgcc
     1141 gaggcgttcg gcccggacat gctgatcgtc gacaaggagc cggtcggcct gcgcggcgag
     1201 ctgacgcaga ccctgaagat gctcaagcgc gacggcacgc gcctggtgct cgggctgcgc
     1261 gacgtgatgg acgagccgcg cctgctcgag ccggagtggc agcgcaagaa ggcgatcccg
     1321 gcgctgcgcg acctgtacga cgagatctgg gtctacggcc tgccgcagat ctgcgacccg
     1381 ctcgagggca tcgcgctgcc ggcgcgcgtg cgccagaaga tggtgtacac cggctacctg
     1441 caccgcgagg tgccgacgca cggcgcgccg ccgcgcctgc cggagatgtg caaccggccg
     1501 ttcctgctgg tcaccaccgg cggcggcggc gacggcgacg ggctgatcga ctgggtgctg
     1561 cgcgcctacg agcacgatcc gctgctgccg taccccgcgc tgctcgtgct cgggccgttc
     1621 atgcagtccg agcgccaggc ggaattcatg gaccgcgcgg cgaagttgaa gcgcgtcgac
     1681 gcgatcacct tccacggcca gctcgaggcg ctggtggacc gcgccgcggg cgtggtggcg
     1741 atgggcggct acaacacctt ctgcgaggtg ctctcgctcg acaagcgcgc gctcatcgtg
     1801 ccgcgcaccg agccgcgcct ggagcaattg atccgcgcct cgcgcgccgc cgagctcggc
     1861 ctggtgagca tgctttccga cgacggcacc tacgaccccg cggtgatggc cgccgcgctg
     1921 cgcgcgctgc cgcgccagaa cctcccgtcc gaggtcgtgg tgcctgggct gctcgaggga
     1981 ctcaagaacg tctcgcggct ggtcgcgcct tcgatcgagc cgcgcgaagt cgaagtggca
     2041 cgcaga
//
LOCUS       DASUNT010000466         2434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_678577, whole genome shotgun sequence.
ACCESSION   DASUNT010000466 DASUNT010000000
VERSION     DASUNT010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678577"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..480
                     /locus_tag="VFX94_09415"
     CDS             49..480
                     /locus_tag="VFX94_09415"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX5768437.1"
                     /translation="MRLRADRGELALALVFATVGALWMAKSARMSLWEGFAPDSGFLP
                     LVYGVLLFFLAAAVAIQLIAEKDERPGEVIRKPLVVLGALIAAVASLPLAGFAISVFA
                     LLLFVYAAVERLRLLPSVLVSGATTGILYVVFKSWLGVPLP"
     gene            477..1955
                     /locus_tag="VFX94_09420"
     CDS             477..1955
                     /locus_tag="VFX94_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5768438.1"
                     /translation="MNELALLASGFAAAFTFKHLVMCFLGVLMGQMVGVLPGIGPSAA
                     IALLLPLTFGADPTSAIILFAGIYYGSQYGGTLTSVLVSVPGESSTVMTSIDGYQLAL
                     QGRAGPALGIAAIGSFIAGTLGTVGLMLLAPALARAALAFGPPEYFALVVLGLTALAA
                     VGSSLLKGLVTGVAGLLLGTIGIDPQNGIARFEFGQTWLLDGVEFIVLTVALFGVGEI
                     IASSVLGVTRPVAEVKSALPTRADWRACRGSILRGTGIGFVIGVLPATGATIASFLAY
                     IVEKKIARDPSRFGKGAIEGVAAPEASNNAAAAGAMVPMLSLGVPGSGTTAIILGALI
                     MFGIRPGPELFTKNADLVWTVIASMYIGNLILLILNLPLAGLFARLLRVPYRWLYPPI
                     LALCIAGVYSQSNSVEDCWLLAGFGALGWLMKRYDWPAAPMILGLVLGPHFETALRQS
                     LTISHVSGVIFVTRPISATLLALALVAVTAPLWMRFVREKPA"
     gene            1939..>2434
                     /locus_tag="VFX94_09425"
     CDS             1939..>2434
                     /locus_tag="VFX94_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX5768439.1"
                     /translation="MKSLLERIGVDINQKLALEAALDWALEQEVPYIDLCLDQTPELL
                     QKDSARLKKARSRLEKAGTRLGLHTLSAVNVAETSPFVAAAADQYLEAYIAAARALGA
                     GWIVVHGGYHFGADKRARMDAAVARIGRACKKAERAGVRLLLENLNPEPDEAEVHYLA
                     ADLDE"
BASE COUNT          322 a          848 c          815 g          449 t
ORIGIN      
        1 ctggcgcgcc aggagaagct cttccgcgag ctgctcggca agtagccgat gcgtcttcgc
       61 gccgacaggg gcgagctcgc gctcgccctt gtttttgcga ccgtcggcgc gctctggatg
      121 gcgaaaagcg cgcgcatgag cctatgggaa ggcttcgcgc ccgactcggg gttccttccg
      181 ctcgtctacg gcgtgctgct tttcttcctc gccgcggcgg tcgcgatcca gctcattgcc
      241 gaaaaggacg aacgccccgg cgaggtcatc cgcaagccgc tggtggtgct cggcgcgctc
      301 atcgccgcgg tcgcgtccct ccccctcgcc gggttcgcga tctcggtgtt cgcgctcctt
      361 ctcttcgttt atgcggcggt cgagcggctg cggctcctcc cgagcgttct ggtttccggc
      421 gcgacgaccg ggatcctgta tgtcgtcttc aagagctggc ttggggtgcc gctgccgtga
      481 acgagctcgc gctcctcgcg agcggcttcg ccgccgcgtt caccttcaag cacctcgtga
      541 tgtgcttcct cggcgtgctg atggggcaga tggtcggcgt gcttccgggg atcgggccct
      601 cggcggccat cgcgcttctc ctgccgctca cgttcggcgc cgatcccacc tcggccatca
      661 tcctttttgc cggcatctac tacggctcgc aatacggcgg gacgcttact tcggtgctgg
      721 tgagcgtgcc cggcgagtcg agcacggtga tgacgagcat cgacggctac cagctcgcgc
      781 tccagggccg cgccgggcct gcgctcggca tcgcggccat cggctccttc atcgccggaa
      841 cgctcgggac ggtggggctg atgctcctcg cgcccgccct ggcgagagca gcgctcgcgt
      901 tcgggccccc ggaatacttc gcgctggtgg tgctcggcct caccgcgctc gcggcggtcg
      961 gctcttccct gctaaaaggg ctggtgaccg gggtcgccgg gctgctcctc ggcacgatcg
     1021 gtatcgatcc gcagaacggc atcgcgcgct tcgagttcgg ccagacctgg ctgctggacg
     1081 gcgtggagtt catcgtcctg acggtcgccc ttttcggcgt gggcgaaatc atcgcgagct
     1141 cggttctcgg cgtaacccgc ccggtcgcgg aggtgaaaag cgcgttgccg acgcgcgccg
     1201 actggcgcgc ctgccgcggc tcgatcctgc gcggcacggg gatcggcttc gtgatcggcg
     1261 tgctgccggc gaccggggcg accatcgcgt ctttcctcgc ctacatcgtt gagaagaaga
     1321 tcgcccggga cccctcgcgc ttcggcaagg gcgcgatcga aggcgtggcg gcgcccgagg
     1381 cgtcgaacaa cgccgccgcg gccggcgcca tggtgccgat gctctcgctc ggcgtgccgg
     1441 gctccggcac caccgccatc atccttggcg cgctcatcat gttcggcatc cggccggggc
     1501 ccgagctctt caccaagaac gccgacctgg tctggacggt gattgcgagc atgtacatcg
     1561 gcaaccttat ccttctgatc ctcaacctgc cgctggcggg gcttttcgcc cggctgctca
     1621 gggtgcccta ccgctggctc tatccgccga tcctcgcgct gtgcatcgcc ggcgtgtatt
     1681 cgcagtcgaa cagcgtcgag gactgctggc tgctcgcagg attcggcgcg ctcggctggc
     1741 tgatgaagcg ctacgactgg cccgcggcgc cgatgatcct cgggctcgtg ctcgggccgc
     1801 acttcgagac ggcgctgcgc caatcgctca ccatctcgca cgtcagcggc gtgattttcg
     1861 tcacgcggcc gatatcggcc acgctgctcg cgctcgccct cgtagccgtc accgccccac
     1921 tatggatgag gttcgtgcgt gaaaagcctg cttgagcgga tcggggtcga catcaaccag
     1981 aagcttgcgc tcgaagccgc gctcgactgg gcgctcgagc aggaggtccc gtacatcgac
     2041 ctttgcctcg accagactcc cgagctcctg caaaaggact ctgcgcggct caagaaagcg
     2101 cgcagccggc tcgagaaagc cgggacccgg ctcggtctgc atacgctttc ggcggtgaac
     2161 gtggcggaaa cctcgccgtt cgtcgccgcg gcggccgacc agtacctcga ggcctacatc
     2221 gccgcggcgc gcgcgctcgg cgctgggtgg atcgtggtgc acggcggcta ccacttcggc
     2281 gccgacaagc gcgcgcgcat ggacgccgcg gtggcgcgga tcgggcgggc ttgcaagaaa
     2341 gcagagcgcg caggcgtgcg gcttctgctt gaaaacctga accccgagcc ggacgaggcg
     2401 gaggtgcact acctcgccgc cgacctcgac gaat
//
LOCUS       DASUNT010000467         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_64960, whole genome shotgun sequence.
ACCESSION   DASUNT010000467 DASUNT010000000
VERSION     DASUNT010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64960"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1720)
                     /gene="argS"
                     /locus_tag="VFX94_09430"
     CDS             complement(80..1720)
                     /gene="argS"
                     /locus_tag="VFX94_09430"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX5768440.1"
                     /translation="MTSDPKGRLAEAYRQALAQEAPEHAGLEVVFEKPRQAEHGDFAC
                     SVALQLAKRLKRNPRQLAAALSARALAAFPDFDNIFAGYDIAGPGFINFRLRPQVKLA
                     AIHEALAAGAGWGRATKQKPLSVQVEFVSANPTGPLHVGHGRQAAIGDALAAILEARG
                     DKVTREFYYNDAGAQINNLALSVQARARGLKPGDAGWPADGYAGEYIEEIARQYRGSL
                     DDLDAIRRFAVDYLRKEQDVDLREFGVKFDVYYLESSLYTEGRVDQVVKALVASGKTY
                     EKDGALWLRSTEYGDDKDRVIRKSDGTYTYFVPDVAYHLTKWRRGFAKVINVQGTDHM
                     GTKARVRAGLQALCAGVPPGYPDYVMHSMVRVMRGGEEVKISKRAGSYVTLRDLLDWV
                     GRDAVRFFLLSRRADADFVFDIDLARAQTEENPVYYVQYAHARVCSVFAQWQGNPGEL
                     AGADLSRLTNEKEFALAQRVGEFPELVAEAARELAPHSLTFYLRSLAADFHSYYNAER
                     ILVDDEALRTARLALCAAVRRTLASGLALIGVSAPEKM"
     gene            complement(1791..>2137)
                     /locus_tag="VFX94_09435"
     CDS             complement(1791..>2137)
                     /locus_tag="VFX94_09435"
                     /inference="COORDINATES: protein motif:HMM:NF037182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX5768441.1"
                     /translation="AGFAQSQLSERRVAGFPPFVFEAALRAEAPKLETALGFLKSAAA
                     GVPAPAEVRVFDPVPHVLTRRAGLERAQLVMHSASRPALQEFLAAVSRKLFEGAPRRV
                     RWHLDVDPIELD"
BASE COUNT          362 a          706 c          702 g          367 t
ORIGIN      
        1 accccgacgc ccatgccggc gaggaaattg ccagcctcgc gcttgcgcgg cttgtgggtc
       61 atcgatcgag acattggcat tacatcttct ccggggcgct gacgccaatg agcgccagcc
      121 cgctggccag cgtccggcgc accgccgcgc agagcgcgag cctcgccgtg cgcagcgcct
      181 cgtcgtcgac gaggatgcgc tcggcattat agtagctgtg aaaatcggcc gcgaggctgc
      241 gcaggtagaa ggtaagcgag tgcggcgcca gctcgcgcgc tgcctcggcg acgagctcgg
      301 gaaactcgcc gacgcgctgt gccagtgcga attccttctc gttcgtgagc cgcgagaggt
      361 ccgcgccggc gagctcgccc ggatttcctt gccactgcgc gaacacgctg cacacgcggg
      421 cgtgcgcgta ctgaacgtaa tagaccgggt tttcctcggt ctgcgcgcgg gcgaggtcga
      481 tgtcgaacac gaagtcggcg tcggcgcgtc gtgagagaag gaagaaccgc accgcgtcgc
      541 gccccaccca gtcgagcagg tcgcgcagcg tgacgtagga gccggcgcgc ttggaaatct
      601 tcacctcttc gccgccgcgc atcacgcgca ccatgctgtg catcacgtag tccggatagc
      661 cgggaggcac gccggcgcag agggcctgca gcccggcgcg cacgcgggcc ttggtgccca
      721 tgtggtcggt gccctgcacg ttgatgacct tcgcgaagcc gcgccgccac ttcgtcaggt
      781 gataggcgac gtccggcacg aaatacgtgt acgtgccgtc cgacttgcgg atgacgcggt
      841 ccttgtcatc gccatactcg gtgctgcgca gccacagcgc gccgtccttc tcgtaggtct
      901 tgccgcttgc gaccagcgcc ttgactacct ggtcgaccct gccttcggta tagaggctcg
      961 actcaaggta gtagacgtcg aacttgacgc cgaattcccg caaatcgacg tcctgttcct
     1021 tgcgcagata gtcgaccgcg aaccggcgta ttgcgtccag gtcgtccagg ctgccgcgat
     1081 actgccttgc gatttcctcg atgtactcgc cggcatatcc atcggccggc cagccggcat
     1141 cgcccggctt gaggccgcgc gcacgggcct gaaccgagag tgcaaggttg ttgatctgcg
     1201 cgccggcgtc gttgtagtag aactcgcgcg tcaccttgtc gccgcgcgcc tccaggatcg
     1261 ccgcgagcgc gtcgccgatc gccgcctgcc ggccgtgtcc gacatgtagc ggcccggtgg
     1321 gattggcgga aacgaactcc acctgaaccg agagcggctt ctgcttcgtc gcacgccccc
     1381 aacccgcgcc ggcagcgagc gcctcgtgga ttgcggcgag cttgacttgc ggtctcagcc
     1441 gaaagttgat gaagccgggt ccggcaatat cgtagcctgc gaaaatattg tcgaaatcgg
     1501 ggaatgccgc gagcgctctg gcgctaagag ctgcggcgag ctgacgtggg ttgcgcttta
     1561 gtcgcttggc gagctgcagc gcgaccgagc aggcgaagtc gccgtgctcg gcctggcggg
     1621 gcttttcgaa cacgacctcc aagccagcat gctcgggcgc ttcttgcgcg agcgcttgcc
     1681 ggtacgcctc ggcaagccgc cctttcggat cggaagtcac tgaaaagtca ggaagtctaa
     1741 tcgaaaaccc attcgggcac ggagccctaa gggctccgtc cccgaacgga tcagtcgagc
     1801 tcgatcgggt caacgtcgag atgccagcgc acccggcgtg gtgccccctc gaacagcttc
     1861 ctcgaaactg cagcaagaaa ttcctgcagc gcgggccgcg aagccgagtg catgacaagc
     1921 tgcgcgcgct cgaggcctgc gcggcgggta aggacatgcg gaacgggatc gaatactcgc
     1981 acctctgcgg gcgccggcac gcccgccgcg gcgcttttca ggaatcccag cgcggtttcg
     2041 agcttcggtg cttcggcacg cagcgccgcc tcgaacacga aaggcgggaa gccggccacg
     2101 cggcgctcgg acagttgcga ttgtgcgaag ccggcat
//
LOCUS       DASUNT010000468         3571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_65215, whole genome shotgun sequence.
ACCESSION   DASUNT010000468 DASUNT010000000
VERSION     DASUNT010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65215"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VFX94_09440"
     CDS             complement(<1..919)
                     /locus_tag="VFX94_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768442.1"
                     /translation="MSRALRRGTPLRSPVDSAEVKRRAKELGADLCGIASAATLNAFP
                     PDPRWPQTPERISPYCKSVVVIVQRIPVGAFRCKTNVPVQYLDMLVLRRMDRIALKLA
                     QELERAGHPSFTLAAQETVWDMKRASYGQLSTRHLGVEAGLGTLGLEVNILTPEFGPR
                     LYLTGILTELELEADRRMEEQVCIGESCSRCLHSCPPDAVRQWGLDKAGCATEAQEFG
                     YMQITKFFGRFIEMNKSEKTSAIRSRDVFGFWQGLLRVVGSFGDCPRCLAVCPVGNDY
                     HAHLADIQKHIPEKTPEKVAKAAKFKQARK"
     gene            complement(932..1360)
                     /locus_tag="VFX94_09445"
     CDS             complement(932..1360)
                     /locus_tag="VFX94_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_267470936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HEX5768443.1"
                     /translation="MRALLASLLLALACAVQAQNNVDASGLALKGYDPVAYFTEGKPV
                     RGKPEYTAAHDGATYRFTSAASRDAFLAAPAKYAPQYGGYCAFGMASGYKAPIEPDAW
                     TIVDGKLYLNYNQSVRTRWSADIPGFIRKADANWPSVRSR"
     gene            1408..2694
                     /locus_tag="VFX94_09450"
     CDS             1408..2694
                     /locus_tag="VFX94_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5768444.1"
                     /translation="MPPPLPLPRSRQEIRRIQSYRKRTAVEQALRSAFWKRRLPKNIS
                     LERLDDADVWRSIPILDKDTLRALTDAEFYGEFCVSGHDGVAEYWRSGSVTGQPLFYP
                     RSHRDIEYGLEAFARTFDCAGARHGDRAHVSFPLGIHPVGQIFARCAASRGITINWAG
                     AGTSTPSALQVELIRRLKPTIWLGMSSYALHLANLAEARGLELRVEKLMTSAEPVSQA
                     KRSKIERSWGARLTDTFGMTEAGMMGAEDRPGAGFRIWTDMYYIEVLDPNTLEPAKEG
                     EIGTLVVTALWSNNITPFLRWSSGDLVTYHEADDGDGAYAVFPRIKHAHRTAGFFKIR
                     GVNVGHQDLEDFMFRHLEIGDFRAEAVNEGGNDILKLSIEIRRGGNPEALVKALIAQV
                     KQTFELMPQIVVLESGTLAREFEANVKAARFVDRRG"
     gene            complement(2701..3069)
                     /locus_tag="VFX94_09455"
     CDS             complement(2701..3069)
                     /locus_tag="VFX94_09455"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX5768445.1"
                     /translation="MITHTGGCHCGRVRFEVQAPAKLDVLECNCSVCSKAGYLHLIVP
                     AERFKLLSGREVLTAYGFNTHTAKHLFCAVCGIKSFYVPGADPNTRSVNVRCLDPGTV
                     ESMRITPYDGRNLKRRRYSP"
     gene            complement(3094..>3571)
                     /locus_tag="VFX94_09460"
     CDS             complement(3094..>3571)
                     /locus_tag="VFX94_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEX5768446.1"
                     /translation="RHRGVTIVWVAHENRADGRDWAGFFDAFVAPGRRAEAAAALAAG
                     SKLQQLWPELDVAAGDLRVAKSRYSAFITGSLKKELEQRGIDTLLLAGTKTTVCVECT
                     ARDAMMLDFKVVLLSDCTAALSDEEHRATLENVIQQFGDVRTVEEALALLEAGGAR"
BASE COUNT          584 a         1193 c         1210 g          584 t
ORIGIN      
        1 tcttgcgcgc ctgcttgaac ttcgccgcct tggcaacctt ttccggcgtc ttctccggaa
       61 tgtgcttctg gatatcggcg aggtgagcgt gatagtcgtt gcccaccggg cacaccgcga
      121 ggcagcgcgg acagtcgccg aaggagccga cgacgcgcag cagtccctgc cagaacccga
      181 acacgtcccg cgagcgaatg gccgaagtct tttcgctctt attcatttcg ataaaccggc
      241 cgaaaaactt ggtgatttgc atgtagccaa actcctgcgc ctcggtggcg cagcccgcct
      301 tgtcgaggcc ccactggcgc accgcgtcgg gcgggcagga gtgcaggcag cggctgcacg
      361 attcgccgat gcacacctgc tcctccatgc gccggtcggc ctcgagctcg agctcggtga
      421 ggatgccggt gaggtagagc cgcggcccga actccggggt gaggatgttc acctcgagcc
      481 cgagcgtccc gaggcccgcc tcgacgccga gatggcgcgt cgagagctgg ccgtagctcg
      541 cgcgcttcat gtcccagacc gtctcctgcg ccgcgagcgt gaaggacggg tggccggcgc
      601 gctcgagctc ctgcgcgagc ttgagcgcga tgcggtccat gcgccgtaac accagcatgt
      661 cgagatactg gaccgggacg ttggtcttgc agcggaacgc accgaccggg atgcgctgca
      721 cgatcaccac cacgctcttg cagtacggcg agatgcgctc cggcgtctgc ggccagcgcg
      781 gatcgggcgg aaacgcgttg agcgtcgcgg ccgaagcgat cccgcaaaga tcggcgccga
      841 gctcctttgc gcggcgcttt acctctgcgg agtcaacagg ggaccgcagc ggggtgccgc
      901 ggcgcagggc acgggacatc cccgccgtgt ctcacctgga acgaaccgat ggccagttgg
      961 cgtccgcctt gcggatgaac cccggaatgt cggccgacca gcgggtgcgc accgactggt
     1021 tgtagttgag gtagagcttg ccatccacga tggtccaggc atcgggttcg atcggcgcct
     1081 tgtagcccga ggccatgccg aaggcgcagt aaccgccgta ttgcggcgcg tacttggccg
     1141 gagcggcgag gaaggcgtcg cggctcgcgg cgctcgtgaa tcggtacgtc gcgccgtcat
     1201 gcgccgcggt gtattcgggc ttgccgcgga ccggcttgcc ctcggtgaag taggcgaccg
     1261 ggtcgtagcc cttcagcgcc agacccgacg catcgacgtt gttctgtgcc tggacggcgc
     1321 aggccagcgc caggagcaag gaagccagga gcgctctcat gcccgttggt cgaggcgggc
     1381 gcgccaaact tacggcagaa tcgggcggtg ccgccgccgc ttccgctccc caggtcccgg
     1441 caggagatcc gccgcatcca gagctatcgc aagcgcaccg cggtcgagca ggcgctgcgc
     1501 tccgcgttct ggaaaaggcg cctcccgaag aatatctcgc tcgagcggct ggacgatgcg
     1561 gacgtctggc gcagcattcc gatcctggac aaggatacgc tgcgcgcgct gaccgacgcc
     1621 gagttctacg gcgagttctg cgtttccggc cacgacggcg tcgccgagta ctggcgctcg
     1681 ggcagcgtca ccggccagcc gctcttctac ccgcgcagcc accgcgacat cgagtacggg
     1741 ctggaagcgt tcgcccgcac tttcgattgc gcgggggcga ggcacggcga ccgcgcgcac
     1801 gtgtcgttcc cgttgggcat ccacccggtg gggcagattt tcgcgcgctg tgccgcctcg
     1861 cgcggcatca cgatcaactg ggccggcgcc ggcacctcga cgccctcggc gctccaggtc
     1921 gagctcatcc gccgcctcaa gcccacgatc tggctcggca tgtcgagcta cgcgctgcac
     1981 ctcgccaacc tcgccgaagc gcgcggcctc gagctgcgcg tggagaagct gatgacgtcc
     2041 gccgagccgg tctcccaggc gaagcgctcc aagatcgagc gcagctgggg cgcgaggctc
     2101 accgacacct tcggcatgac cgaggccggc atgatgggcg cggaggaccg gccgggtgcc
     2161 ggcttccgca tctggaccga catgtactac atcgaggtgc tcgatcccaa taccttggag
     2221 ccggcgaagg agggcgagat cggcacgctg gtcgtcacgg cgctctggtc caacaacatc
     2281 acgccgttcc tgcgctggag ctcgggcgac ctcgtcacct atcacgaggc cgacgacggc
     2341 gacggcgctt acgcggtctt cccgcgcatc aagcacgccc accgcaccgc cggcttcttc
     2401 aagatccgcg gcgtcaacgt cggccaccag gacctcgagg acttcatgtt ccgccacctc
     2461 gagatcggcg acttccgcgc cgaggcggtg aacgagggcg gaaacgacat cctgaagctg
     2521 tcgatagaga tccggcgcgg cggcaacccg gaggctctcg tgaaagcctt gatcgcgcag
     2581 gtcaagcaga ccttcgagct catgccccag atcgtcgtgc tggaatcggg gacgctcgcg
     2641 agggagttcg aggccaacgt taaggccgct cggttcgtcg atcgccgcgg ttgagcgccg
     2701 tcacggcgaa tatctgcgtc gtttcaagtt gcgcccgtcg tagggcgtga tcctcatcga
     2761 ctcgaccgtg ccgggatcga ggcagcgaac gttcacgctg cgcgtgttcg gatccgcacc
     2821 cggcacgtag aaggacttga tgccgcacac cgcgcagaag aggtgcttgg cggtgtgagt
     2881 gttgaagccg taggccgtga gtacctctct gccggaaagc agcttgaagc gctcggccgg
     2941 gacgatgaga tgcagatagc ccgccttgct gcataccgag caattgcatt cgagcacgtc
     3001 gagcttcgcc ggcgcctgga cctcgaacct gacgcggccg caatggcagc cgccggtgtg
     3061 cgtgatcatg ctcctatcgc gggccttgcc acctcaacgc gcaccgccgg cctcgagcaa
     3121 ggcaagcgcc tcttcgacgg tgcgcacgtc gccgaactgc tggatcacgt tctcgagcgt
     3181 cgcgcggtgc tcttcgtcgg agagcgcggc cgtgcagtcg gagagcagaa ccactttgaa
     3241 gtcgagcatc atcgcgtcgc gcgcggtgca ctcgacgcag acggtggtct tggtaccggc
     3301 gaggaggagc gtgtcgatgc ctctctgttc gagctctttc ttcaaggaac cagtgataaa
     3361 ggcgctgtag cggctcttgg cgacgcgcag gtcgccggcc gccacgtcga gctccggcca
     3421 gagctgctgg agcttcgagc cggcggcgag cgcggcggca gcctcggcac gccggccggg
     3481 ggcgacgaag gcgtcgaaga agccggccca gtcgcggccg tcggcgcggt tctcgtgcgc
     3541 cacccagacg atggtcacgc cccggtgccg g
//
LOCUS       DASUNT010000469         2648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_241353, whole genome shotgun sequence.
ACCESSION   DASUNT010000469 DASUNT010000000
VERSION     DASUNT010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241353"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFX94_09465"
     CDS             <1..309
                     /locus_tag="VFX94_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3641 domain-containing protein"
                     /protein_id="HEX5768447.1"
                     /translation="GSMLVSKGKFGAYMELLHAAHREENLASVMCRALVSVDWQGYVY
                     DCDFNQMLGLPLVVDARRRVRLTDLLARELAGNPIAVRDHCFGCTAGQGSSCGGALAA
                     "
     gene            306..2432
                     /locus_tag="VFX94_09470"
     CDS             306..2432
                     /locus_tag="VFX94_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5768448.1"
                     /translation="MSRTRLAVAVLFVLAVAAFFAAGGARYFTFENLKAQQATLEAWR
                     ALHPWAAALGFLALYIAFTSLSLPAASLLTILAGAIFGLALGTVLVSFASAIGATIAM
                     LASRFVLREWVQARFAAPMKGVNRGVAREGAFYLFTLRLIPAVPYFLINLAMGLTAMR
                     TWTFYWVSQVAMLPATMLYVNAGTQLARLESLRGILSWQLIGAFALLGIFPLAAKKAL
                     DFVAARRVYARWRKPRRFDRNLVVIGGGSAGLVAAYIAAAVRAKVTLVEKHRMGGDCL
                     NTGCVPSKALIKTARVLYLLRTSESFGIRKATAEFDFAEAMERVQRVIRAIEPHDSAE
                     RYTALGVECLKGEARMTNPWTVEVDGKALTTRSIVIAAGAEPFIPPIPGIDTVECLTS
                     DTVWALRALPRRLLVLGGGPIGCELAQAFARLGSNVTQVEMLPRLLPREDPEFSEMVA
                     SRMRQDGVNVLVGHKAKQFFPEKILVCEHHGSEVRIAFDALLCAVGRVARTKGYGLEE
                     LGIPLTKARTVETNEFLQTLYPNIYACGDVAGPYQFTHVGAHQAWYAAVNALFGWLKK
                     SRADYSVIPWATFTDPEVARVGLNEAEAKERNIAYEVTVYDLGDLDRAIADGTAHGRV
                     KVLTVPGTDRILGATIAGEHAGDLLSEFVSAMRHGLGLNKILGTIHIYPTLAEANKYA
                     AGAWKKAHAPQRVLAAMERLHAWRRG"
     gene            2429..>2648
                     /locus_tag="VFX94_09475"
     CDS             2429..>2648
                     /locus_tag="VFX94_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768449.1"
                     /translation="MKKICLAALLMLALPAWAFDRSHKSWDAMLKKHVVLLEGGKASQ
                     VNYQGMATERAALTEYLHSLSRVGEQEFE"
BASE COUNT          411 a          898 c          877 g          462 t
ORIGIN      
        1 ggctcgatgc tcgtgtcgaa gggcaagttc ggcgcctaca tggagctgct gcacgccgcg
       61 caccgcgagg agaacctcgc ctcggtgatg tgccgcgcgc tcgtttcggt cgactggcag
      121 ggctacgtgt acgactgcga tttcaaccag atgctcggat tgccgctcgt agtagacgca
      181 cgccggcgcg tgcggctcac cgatctcctc gcccgcgagc tcgccggcaa cccgatcgcc
      241 gtgcgcgatc actgtttcgg ctgcaccgcc ggccagggct cttcctgcgg cggcgcgctg
      301 gcggcttgag ccgcacccgg ctcgccgtcg cggttctctt cgtcctcgcc gtcgccgcct
      361 tcttcgcggc cgggggcgcg cgctatttca ccttcgagaa cctgaaggcc cagcaggcga
      421 cgctggaggc ctggcgcgcg ctgcatccct gggcggccgc cctcggcttt cttgcgctct
      481 acatcgcgtt cacctctctc tccttgcccg ccgcctcgct gctgacgatt ctcgccggcg
      541 cgatcttcgg cctggcgctc ggcaccgtgc tcgtctcgtt tgcctccgcc atcggcgcga
      601 cgattgccat gctggcgtcg cggttcgtgc tgcgcgaatg ggtgcaggcc cgcttcgccg
      661 ccccgatgaa gggcgtgaac cgcggcgtgg cgcgcgaggg agcgttctat cttttcacgc
      721 tgaggctcat tccggcggtg ccttactttc tcatcaacct cgcgatgggc ctcactgcga
      781 tgcgcacctg gaccttctac tgggtgagcc aggtcgccat gctgcccgcg acgatgctct
      841 acgtgaacgc gggaacgcag cttgcgcgcc tggagtcgct gcgcggcatc ctgtcctggc
      901 agctcatcgg cgcgttcgcg ctcctcggca ttttcccgct cgcggcgaaa aaggcgctcg
      961 atttcgtcgc ggcacgccgc gtgtacgcgc gctggcgaaa accgcgccgc ttcgaccgca
     1021 atttggtcgt catcggcggc ggatcggcgg gcctggtcgc cgcgtacatc gctgccgcgg
     1081 tgcgcgccaa agtgaccctc gtcgagaagc accgcatggg cggggactgc ctgaacacgg
     1141 ggtgcgtgcc ctccaaagcg ctgatcaaga cggcaagggt gctgtatctg ttacgaacct
     1201 ccgaatcatt cgggatccgc aaagcgaccg cggaattcga cttcgccgag gcgatggagc
     1261 gcgtgcagcg ggtgatccgc gcgatcgagc cgcacgactc ggccgaacgc tacaccgccc
     1321 tgggtgtcga atgcctcaag ggcgaagcgc gcatgaccaa cccctggacc gtggaagtgg
     1381 acggcaaggc tctcaccacc cgctcgatcg tgattgcggc cggcgcggag cctttcattc
     1441 cgcctattcc gggcatcgat acggtcgaat gcctaacctc ggacaccgtc tgggcactgc
     1501 gcgcgctgcc gcggcggctg ctcgtgctcg gcggcgggcc gatcggctgc gagctcgcgc
     1561 aggcgtttgc ccgcctgggc tcgaacgtca cgcaggtcga gatgctgccg cgcctcctgc
     1621 cgcgcgaaga cccggagttt tcggaaatgg tggcaagccg catgcgccaa gacggcgtca
     1681 acgtgctcgt cggccacaag gcgaagcagt tcttcccaga aaagatcctg gtatgcgagc
     1741 accatggcag cgaggtgcgc atcgcgttcg atgcgctgct ctgcgccgtc ggccgcgtgg
     1801 cgcgaaccaa gggctacggc ctcgaagagc tcgggatccc gctcaccaag gcgcgcacgg
     1861 tggagaccaa cgagttcctg cagacgctct atcccaacat ctatgcgtgc ggcgacgtcg
     1921 cgggccctta ccagttcacg cacgtaggcg cgcaccaggc gtggtacgca gcggtgaatg
     1981 cgctcttcgg ctggctgaag aagtcccgcg ccgattactc ggtgatcccg tgggcgacgt
     2041 tcaccgatcc ggaagtggcg cgcgtcgggc tgaacgaggc cgaggcgaag gagcgcaaca
     2101 tcgcgtatga agtcacggtc tacgatctcg gcgatctcga ccgcgccatc gccgacggca
     2161 ccgcacacgg acgcgtgaag gtgctcaccg tgccggggac cgaccgcatc ctgggcgcca
     2221 cgatcgccgg cgagcacgcc ggcgatcttc tcagcgagtt tgtctcggcg atgcgccacg
     2281 gtctgggcct gaacaagatc ctcgggacga tccacatcta tccgacgctc gccgaggcga
     2341 acaagtacgc cgcgggcgcg tggaagaaag cgcacgcccc gcagcgggta ctcgccgcga
     2401 tggaacggct gcatgcgtgg cggcggggat gaaaaagatc tgtctcgccg ccttgctcat
     2461 gctcgcccta ccggcatggg cgttcgaccg gtcgcacaag tcgtgggacg cgatgctcaa
     2521 gaagcacgtg gttctgctcg agggcggcaa ggcttcgcag gtgaactacc agggcatggc
     2581 aacagagcgc gccgccctga ccgaatacct gcactccttg tcgcgcgtgg gcgagcagga
     2641 gttcgagg
//
LOCUS       DASUNT010000470         2317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_417071, whole genome shotgun sequence.
ACCESSION   DASUNT010000470 DASUNT010000000
VERSION     DASUNT010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417071"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VFX94_09480"
     CDS             complement(<1..893)
                     /locus_tag="VFX94_09480"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX5768450.1"
                     /translation="MLLGYLTVRFPSLTAIAGTVSLETMTVSAGITALTIEATRRTIG
                     WSLVIILLALAGYVLIGHLVPGQLQTRKVDLAQLLGYLNLDNNGLLGLVLQITVIVII
                     PFVLMGQLLMRSGGSNFFNDAAIALMGRYRGGAAKMAVVGSSLFGMISGIAAANTVAV
                     GIVTIPLMKRSGMSPRLAGAVEACASNGGQLMPPVMGAVAFVMADFLQIPYKDVAIAA
                     ILPSVMYYAALFIQTDLEAARYGFGRVEQDKIPRIGSVLAKGWLFLVPFAVLIYTMFW
                     LNLEPEYSAMAASLAIIVLGFA"
     gene            complement(1082..2041)
                     /locus_tag="VFX94_09485"
     CDS             complement(1082..2041)
                     /locus_tag="VFX94_09485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX5768451.1"
                     /translation="MLKAARSIACSAMLCCASFAWAQTVGFATLPPGSILHAQASVIA
                     KVVQDNAKLQVRVIGYGGDTGILEAVSSKKDDFWLLDVGESADALHGRGTWKGNAKPN
                     LRTAITMYGFQMGFWVRRDSSINSIADLKGKRVPSEWVQQTGVLPHTTAVLAAGGLTY
                     NDVVKVPEVNVVRAADDFKAGKLDLLFFAVGAPKVQEVAAAVGGLRLLPMNPLPDSEK
                     RMKAVRPEYYFSSVNPAPHIAGIEKPSQVQTIDVVIGVGAHVSDEVVYQFVKAMRENK
                     KALAAGHPNFNRFDEAEAGKAQPSLPHHPAAIKYFKEVGVWRG"
     gene            2084..>2317
                     /locus_tag="VFX94_09490"
     CDS             2084..>2317
                     /locus_tag="VFX94_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX5768452.1"
                     /translation="MKLPKDRVPYSAIVDRPALKLPNDARIAVWTIVNVEEWSIERAM
                     PRMVLSPPQGQPLLPDLPNWAWHEYGMRVGFWRF"
BASE COUNT          413 a          766 c          760 g          378 t
ORIGIN      
        1 gcgaagccga gcacgatgat cgccaggctc gcggccatcg ccgagtactc gggctcgagg
       61 ttcagccaga acatggtgta gatcagcacc gcgaacggca cgaggaagag ccagcccttc
      121 gccagcacgc ttccgatgcg cgggatcttg tcctgctcca ccctgccgaa gccgtagcgc
      181 gcggcctcga ggtccgtctg gatgaagagc gctgcgtagt acatcaccga gggcaggatc
      241 gccgcgatcg ccacgtcctt gtagggaatc tgcaggaagt cggccatcac gaaagcgacg
      301 gcccccatca ccggcggcat gagctgcccg ccgttggagg cgcacgcttc caccgcgccg
      361 gcgagccgcg gcgacatgcc ggagcgcttc atgagcggaa tggtgacgat gcccacggca
      421 acggtgttcg ccgccgcgat gccggagatc atgccgaaca ggctcgagcc cacgaccgcc
      481 atcttcgccg cgcctccgcg gtagcgtccc atgagcgcga tcgcggcgtc gttgaagaag
      541 ttggagccgc ccgagcgcat gaggagctgt cccatcagca cgaacgggat gatcacgatc
      601 acggtgatct gcagcaccag gccgagcagc ccgttgttgt cgaggttgag gtagccgagg
      661 agctgcgcga ggtccacctt gcgcgtctgg agctgtcccg gcaccaggtg gccgatgagc
      721 acgtagcccg ccagcgcgag caggatgatg acgagcgacc agccgatcgt gcgccgcgtc
      781 gcctctatcg tgagcgccgt gatgccggcg gaaacggtca tcgtttccag gctcaccgtg
      841 cccgcgatcg cggtgaggct cggaaaccgg acggtgaggt aaccgagcag caccagcgct
      901 gcgacggcga gcagccagtc gagcttccac gccctgaagc gcagaaaggt gagcgcgagg
      961 ccgatgccgt acgcggcggc ggcgaactgc tcggcgaaga ggaccagccc gagcggccgg
     1021 aaaaggtcga gcgaccagcc gatcgagcct gcgataagga acgcggctag aacactttgc
     1081 gctacccgcg ccagacgccg acttccttga agtacttgat ggccgcggga tggtgcggca
     1141 ggctgggctg cgccttgccg gcctccgcct cgtcgaagcg gttgaagttc gggtggccgg
     1201 cggcgagcgc cttcttgttc tcgcgcatcg ccttgacgaa ctgatagacc acttcatcgg
     1261 acacgtgggc gccgacgccg atcaccacgt cgatcgtctg cacctgcgag ggcttctcga
     1321 tgcccgcgat atgcggcgca gggttcacgc tggaaaagta gtactcggga cgcaccgcct
     1381 tcatgcgctt ttcggagtcg ggcagcgggt tcatcggcag caggcgaagg cctcccaccg
     1441 cagcggcgac ttcctgcacc ttcggcgcgc cgaccgcgaa gaagagaagg tcgagcttgc
     1501 ccgccttgaa atcgtcggcg gcgcgcacga cgttcacttc gggaaccttg acgacgtcgt
     1561 tataggtcag cccgcccgcg gcaaggaccg cggtggtatg cggcaggacg cccgtctgct
     1621 gcacccactc ggagggcacg cgctttccct tcaggtccgc gatcgaattg atgctcgagt
     1681 cccttcgaac ccagaatccc atctggaacc cgtacatggt gatcgcggtg cgcaggttcg
     1741 gcttcgcgtt gcctttccac gtgccccggc cgtgcagcgc gtcggcgctc tcgcccacgt
     1801 cgaggagcca gaagtcgtcc ttcttcgagc tcaccgcctc caggatcccc gtatcgccgc
     1861 cgtagccgat gacgcgcacc tgcagcttcg cattgtcctg gacgaccttt gcgatcacgg
     1921 aggcctgcgc atgcaggatc gatcccggcg gcagcgtggc gaagccgacg gtctgcgccc
     1981 acgcgaacga ggcgcagcag agcattgccg agcaagcgat cgacctcgcg gctttcaaca
     2041 tgttgtcctc ctccaaggaa cttagtcggt atattagccg aggatgaagc ttcccaagga
     2101 ccgcgtgccg tactcggcga tcgtcgaccg gccagcgctc aagcttccca acgacgcgcg
     2161 catcgcggtc tggacgatcg tcaatgtcga agagtggtcc atcgagcgcg ccatgccgcg
     2221 catggtcctc tcgccaccgc agggacagcc gctgctcccc gacctgccga actgggcctg
     2281 gcatgagtac gggatgcggg tgggtttctg gcggttt
//
LOCUS       DASUNT010000471         3814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_330127, whole genome shotgun sequence.
ACCESSION   DASUNT010000471 DASUNT010000000
VERSION     DASUNT010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330127"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VFX94_09495"
     CDS             <1..521
                     /locus_tag="VFX94_09495"
                     /inference="COORDINATES: protein motif:HMM:NF014755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX5768453.1"
                     /translation="DIAPQYFDTPYYLVPGKRGEKAYALLRDTLSKAGKAGIASVVIR
                     TKQYLAALVAQDALLVLNTLRYHDELKDPSELEVPEAKVSAKELDMAMRLVEDMADEW
                     EPGRYRDTFRDDLLKRIEEKVKAGQTDEITEPEKERTAAKGAEVIDLMELLKKSIEKK
                     PAKRPARKRRAA"
     gene            539..745
                     /locus_tag="VFX94_09500"
     CDS             539..745
                     /locus_tag="VFX94_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768454.1"
                     /translation="MATCAVCGNSYDKAFSVRMPDGAAHTFDSFECAIAKLAPTCAHC
                     NCRVIGHGVERDGKVFCCEHCAHA"
     gene            738..1829
                     /locus_tag="VFX94_09505"
     CDS             738..1829
                     /locus_tag="VFX94_09505"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5768455.1"
                     /translation="MPKRQIRYAVVGLGHIAQVAVLPAFANARRNSRLAALVSGNAAK
                     REQLGARYQVEKSWSYAEYDACLKSVDAVYIALPNHMHCEYAVRAARAGVHILVEKPM
                     ALTEEECGRMIGAAREAGVKLMVAYRLHFERANLEAGEIARSGRIGEPRLFNSTFCTP
                     VAPGNIRVRRETGGGVLYDIGIYCINAARSLFRDEPVEVRAILSGRLDEVEESASAVL
                     RFPNDRIAGFTASFGTEKVSEYRLAGTKGDLAVEPAYDYAKPLKHRLTLEGEMRERRF
                     AKRDQFAPELLYFSDCILQNGTPEPGGDEGLADVRVIRALYRSAQSGQPVALEPFDKR
                     ARPSLEQEIRRPPVEKPEVIHAAAPSGPS"
     gene            1860..2222
                     /locus_tag="VFX94_09510"
     CDS             1860..2222
                     /locus_tag="VFX94_09510"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5768456.1"
                     /translation="MKALIAVAVAAALVLTACASQERSGTSSSGQTSGSSAGSGQSSG
                     ASASSNAMLTGKVKSALAADVGLGTLTSIDVDSDNGVVTLKGHVDSADKKAKAEEVAK
                     KVEGVKSVKNELRVEKSS"
     gene            2225..2455
                     /locus_tag="VFX94_09515"
     CDS             2225..2455
                     /locus_tag="VFX94_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768457.1"
                     /translation="MDDSLTPLLSVTQTRDGVWSVNEREPTRVAIAYFPTKWGAMRHA
                     VRVARARHRCRVALVDRGRGLLLSHDYSQDSP"
     gene            2452..2961
                     /locus_tag="VFX94_09520"
     CDS             2452..2961
                     /locus_tag="VFX94_09520"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEX5768458.1"
                     /translation="MRTSTFALVIGIVYLAVAVLGLVPALLMPPPADAPPTAFTVLYG
                     YLIGLFPLNVLHTAVHFAIGVWGIAAGRGAASAKVYARSLAILYGVLAVMGLIPGLNT
                     VFGLVPLHGHDVWLHAATAAIAAYFGWRKTTDLERRAASERRQRMIPVARERRLGATE
                     RRQVSYNGA"
     gene            3456..>3814
                     /locus_tag="VFX94_09525"
     CDS             3456..>3814
                     /locus_tag="VFX94_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEX5768459.1"
                     /translation="MAAPPFRADHVGSLLRPQELRQAREAFMAGKLTREALTAVEDDS
                     IRKVIALQEAAGLQSVTDGEYRRAFWHVDFLTGFDGIVATQGQYALKFHGEGGAQSET
                     RSMMAVSGKVRRSRPIAL"
BASE COUNT          669 a         1299 c         1294 g          552 t
ORIGIN      
        1 ccgacatcgc gccgcagtac tttgacactc cctattacct cgtcccgggc aagcgcggcg
       61 agaaagcata tgcgctgctg cgcgacacgc tgagcaaggc gggcaaggcc ggcatcgcga
      121 gcgtcgtgat ccgcaccaag cagtacctgg cggcgctggt ggcgcaggac gccctcctcg
      181 tgctgaacac gctgcgctac cacgacgagc tcaaggatcc ctcggagctc gaggtccccg
      241 aggcgaaggt ctccgcgaag gagctcgaca tggcgatgcg cctggtcgag gacatggccg
      301 acgagtggga acccggccgc taccgcgaca ctttccggga cgatctcctg aagaggatcg
      361 aggaaaaggt gaaggccggc cagaccgacg agatcaccga gccggagaag gagaggacgg
      421 ccgcgaaagg cgccgaggtg atcgatctca tggagctgct caagaagagc atcgagaaga
      481 aacccgcgaa gcgccccgca cgaaagcggc gcgccgcatg acccggaaag gaaccgacat
      541 ggcaacgtgc gcagtgtgcg gcaacagcta tgacaaggcg ttctcggtgc gcatgcccga
      601 cggcgccgcg cacaccttcg acagcttcga atgcgcgatc gcgaagctcg cgccaacctg
      661 tgcccactgc aactgccggg taataggcca cggcgtagaa cgcgacggca aggtgttctg
      721 ctgcgagcac tgcgcgcatg cctaagcggc agattcgcta cgcggtggtc gggctcgggc
      781 acatcgccca ggtggcggtg ctgccggcgt tcgccaacgc ccggcgcaat tcccggctgg
      841 ccgcgctcgt atccgggaac gcggcgaagc gcgagcagct cggcgcgaga taccaggtgg
      901 aaaagagctg gtcctacgcg gagtacgacg cctgcctgaa gagcgtggac gcggtgtaca
      961 tcgcgctgcc caaccacatg cattgcgagt acgcggtgcg cgctgcgcgc gccggggtcc
     1021 acatcctcgt cgagaagccg atggcgctca cggaggagga gtgcgggcga atgatcggcg
     1081 cggcgcgcga ggccggcgta aagctcatgg tggcctaccg gctgcatttc gagcgggcga
     1141 acctcgaggc gggcgagatc gcgcgctcgg ggcgcatcgg cgagccgcgc ctcttcaact
     1201 ccaccttctg caccccggtg gcgccgggca acatccgcgt gcgccgcgag accggcggcg
     1261 gcgtgctcta cgacatcggg atctattgca tcaacgccgc gcgctcgctc ttccgcgacg
     1321 agccggtgga ggtgcgcgcg atattgtccg ggcggctcga cgaggtcgag gagtcggcct
     1381 cggcggtgct gcgcttcccg aacgaccgca tcgccgggtt taccgcgtcc ttcggcacgg
     1441 aaaaggtctc ggaataccgc ctcgccggca cgaagggcga cctcgcggtc gagccggcgt
     1501 acgactacgc caagccgctc aagcaccgcc tgaccctcga gggcgagatg cgcgagcggc
     1561 gcttcgccaa gcgcgaccag ttcgcccccg agctgctcta tttctcggac tgcatcctgc
     1621 agaacggcac gcccgagccc ggcggcgacg aaggcctcgc cgatgtgcgg gtgatccgcg
     1681 cgctctaccg ctcggcgcaa agcgggcagc cggttgcgct cgagccgttc gacaagcgcg
     1741 cgcggccgag cctcgagcag gagatccgtc gccccccggt ggagaaaccc gaggtgatcc
     1801 acgccgcggc gccgtcaggc ccaagctaga ttttcttccc taacgaaagg agaaagacca
     1861 tgaaagccct catcgccgtc gcagttgcag cagcgctggt tctgaccgcg tgcgcctctc
     1921 aggagagaag cggcaccagc agcagcggac agacttccgg cagcagcgcc ggcagcggac
     1981 agagctccgg cgccagcgcc tccagcaacg ccatgcttac cggcaaggta aagagcgcgc
     2041 tcgccgccga cgtcggcctg ggcaccttga ccagcatcga cgtcgacagc gacaacggcg
     2101 tggtgacctt gaagggccac gtcgactcgg ccgacaagaa ggccaaggcc gaggaggtcg
     2161 cgaagaaggt cgaaggcgtg aagtcggtga agaacgagct acgcgtcgag aagagctcgt
     2221 aagcgtggac gactccttga cgccgttgct ttcggtcacg cagacgcgcg acggcgtctg
     2281 gagcgtcaac gagcgcgagc cgacgcgcgt ggcgatcgcg tatttcccca ccaagtgggg
     2341 cgcgatgcgc cacgcggtgc gcgtcgcccg cgcccggcac cgctgccgcg tggccctcgt
     2401 ggaccgcggc cgcggcctgc ttctctcgca cgactactcg caggactcgc catgagaacc
     2461 tcaacgttcg ctctcgtcat cggcatcgtg tacctcgcgg tcgcagtact ggggctggtt
     2521 ccggcgcttc tcatgccgcc tcccgccgac gcgcctccga ccgccttcac ggtgctgtac
     2581 ggttacctga tcgggctctt cccgctgaac gtcctgcata cagcggttca cttcgcgatc
     2641 ggcgtgtggg gtatcgccgc cggacggggt gccgcgagcg cgaaggtcta cgcgcgctcg
     2701 cttgcgatcc tgtacggcgt gctggccgtg atggggctca tacccggact caacaccgtg
     2761 ttcggccttg tcccgctgca cggccacgat gtgtggctgc atgccgccac ggcggccatt
     2821 gccgcgtact tcggctggcg caagacgacc gatttggaac gcagagcggc aagcgagcgc
     2881 cgtcagcgca tgatccccgt cgcgcgtgag cgccggctcg gcgccacgga gcgccgccag
     2941 gtttcgtaca acggcgcatg agaacgggca agacgctccg cagctgggcc tccggccggt
     3001 cgcggcgcga ggtcacccgg cgcgccgccg cggacctgga gcgcgggctc gtggacaccg
     3061 accgccgcgg gaaaccggtc ccggcaccga agcggcggaa gaagtagtgc ttccggtgca
     3121 cgatgtcgat tgcccccggt gcgggcagcg cctgtacacg gccatttccc tcgaggatgt
     3181 cgtccctgcc gacgcgccca ccgcgccgaa ggtggtaagt gatgcgcgcg gctactacct
     3241 gcgctgcccg cactgccagg cccgaatcga aatgaagcgt atcgaaggcc ccggcgggat
     3301 cggcttccgc gtcgcataga cacggctcgt cgtccccgga aggctgtcgt tcccgcgacg
     3361 gcgggaaccc aatttgatcc ccgccccgct ttcgcgggga agacagctca gcgctccctc
     3421 gcccttgggg cgcttcccac tgtagtctgc gcccaatggc ggccccgccc tttcgcgccg
     3481 accacgtcgg aagcctcctg cgcccgcagg aactcaggca ggcgcgcgaa gcgttcatgg
     3541 caggcaagct cacgcgcgag gcgctcactg ccgtggaaga cgattccata agaaaagtga
     3601 tcgcgctgca ggaagccgcc gggctgcagt cggtcaccga cggggaatac cggcgcgcgt
     3661 tctggcacgt cgacttcctc accggcttcg acggaatcgt ggcgacccag ggacagtacg
     3721 cgctcaagtt ccatggcgag ggcggcgcgc agtccgagac gcgctccatg atggcggtga
     3781 gcggcaaggt ccgccgctcc cgcccgattg cgct
//
LOCUS       DASUNT010000472         3881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1029612, whole genome shotgun sequence.
ACCESSION   DASUNT010000472 DASUNT010000000
VERSION     DASUNT010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029612"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1421
                     /gene="glp"
                     /locus_tag="VFX94_09530"
     CDS             171..1421
                     /gene="glp"
                     /locus_tag="VFX94_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX5768460.1"
                     /translation="MSITQTLSCLDGYDPDALRVDKAREAIRSCLIQIQQIEKVTIRE
                     ALGRVLAEDIVPQINVPAHDNSAMDGYAVRFSDLEKSASLKEVGTALAGKPFAGKLGA
                     GECVRIMTGAVLPEGADTVVIQEVVKKAGDRVTIPPGQKKAQNVRYAGEDLRVGKSVL
                     GTGKLLTPADIGLVASLGIGEVDVKRRLRVAFFATGDELASIGAPLKEGEVYDSNRYT
                     LHGMLSRLGVELIDMGVVRDAPHLLEHAFRHASEQAHAVITTGGVSVGEADFVRQLMN
                     RLGEVLFWKIAMRPGRPMAFGRIGNAFLFGLPGNPVAVMVTFYQFVRDALLFMSGRES
                     GEMPLLSAIAAENLRKVPGRTEYQRGVIFKDQDEWKVRTTGQQGSGVLRSMSEANCFI
                     VLEHERANVKAGELVSVQPFEGLA"
     gene            1421..2044
                     /locus_tag="VFX94_09535"
     CDS             1421..2044
                     /locus_tag="VFX94_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5768461.1"
                     /translation="MALVVLSSLALKGVLEKSRAQLEESLGTRLELHFDATQAIVPRL
                     AAGFRADLVILTREAIEELHTAGRVTQVRDVGSSGVGVAVRAGARKPDIGSVEALKHA
                     LVSANSVAHSKVGASGIYFVELLERLDIRLKKRVVVDKGPVGLAVASGEAELGVQQLC
                     ELEPVPGIEIVGPLPDAVQKITYFSAGIPVHAAHAASARALMEALQL"
     gene            complement(2041..3015)
                     /locus_tag="VFX94_09540"
     CDS             complement(2041..3015)
                     /locus_tag="VFX94_09540"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX5768462.1"
                     /translation="MLKKIAVLTSGGDAPGMNAAIRAVTRGALARGWQVAGVRNGYAG
                     LLGGHFEPLAARDVGGIMQSGGTVLGSARCPEFAEPAGRRQAIANLKKEDVEALVVIG
                     GNGSQTGSAALTRDGFAVVGVPSTIDNDLYGNDVSIGCDTAINITLEAIDRLRTTASS
                     HQRAFAVETMGRDCGYIALIAGIAGGAEVIAIPEVDITPAHIAERLRDAYRRGKTHAL
                     AVIAEKARCGVHELMEYYKTNRALIGFDLRVTRLGHVVRGGTPGAADRVLATRLGAAA
                     IDTLAAGGHGVLVGIVRGDIVTTPLSEIDGRTRPADASLLELARVMAI"
     gene            3033..3626
                     /locus_tag="VFX94_09545"
     CDS             3033..3626
                     /locus_tag="VFX94_09545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX5768463.1"
                     /translation="MGKPLDELRTAARGCQDCPLWANATQTVFGAGDPHARVMLVGEQ
                     PGDEEDKKGLPFVGPAGRLLDRALEAAGVEREHLYVTNAVKHFKWQLRGKRRLHKTPA
                     QREIEACHQWLEREIESIEPHVIVALGSTAAKAVIARDFKVSVQRGQFVDSPLAPYVF
                     ATFHPSALLRLREDEEREVAFKQLVTDLKLIKKALAR"
     gene            complement(3623..>3881)
                     /locus_tag="VFX94_09550"
     CDS             complement(3623..>3881)
                     /locus_tag="VFX94_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX5768464.1"
                     /translation="LLGEEAAMARFEDRLARAFSSVINILDPDVIVVGGGMSKHESIY
                     TNVAPLLRKYVYHREPRTPIVRALHGDASGVRGAALLWPLS"
BASE COUNT          665 a         1238 c         1346 g          632 t
ORIGIN      
        1 gtgttcgcgc tcatgaagcg cgaggtgcgc gaaagcctgg aagcttttct cacccagggc
       61 ggccggaaaa tcgacggctg gtacgccgcg ctcaaggtcg tggaagtgag cttcgacgac
      121 gaagcacaag cgtttcgcaa catcaatacg ctggacgagc taaatcgcga atgagcatca
      181 cgcaaacgct gagctgcctc gacggctacg atcccgacgc gctgcgcgtc gacaaggcgc
      241 gcgaagcgat aagaagttgt ttgattcaga ttcaacaaat cgagaaggtg acgatccgcg
      301 aggcgctggg ccgcgtgctg gccgaggaca tcgtgccgca aatcaacgtc ccggcgcacg
      361 acaactcggc gatggacggc tatgccgtgc gcttttccga ccttgaaaaa agcgcttcat
      421 tgaaggaggt cggaaccgcg cttgccggca agccgttcgc gggaaagctc ggagcgggcg
      481 agtgcgtgcg catcatgacc ggcgcggtgt tgcccgaggg cgcggatacc gtcgtgatcc
      541 aggaggttgt gaagaaggca ggcgaccgcg tcaccatccc gcccggacaa aagaaggcgc
      601 agaacgtgcg ctacgccggc gaagacctca gggtcggcaa gtcagtgctc ggaaccggaa
      661 aactgctcac acccgccgac atcggactag ttgcttcgct cggcatcggc gaagtcgatg
      721 taaagcgcag gctgcgagtg gcgttcttcg ctaccggcga tgagctcgcc tcgatcggcg
      781 cgcccctcaa ggaaggcgag gtctacgact ccaaccgcta tacgctccac ggaatgctgt
      841 cccggctcgg cgtggagctc atcgacatgg gcgttgtgcg cgatgcgccg cacctgctgg
      901 aacacgcttt ccgccacgcc tccgagcagg cgcacgccgt aattacgact ggaggcgtct
      961 ccgtagggga agccgacttt gtccgccagc tcatgaacag gctcggcgag gtgctgttct
     1021 ggaagatcgc catgcgcccc ggccggccga tggccttcgg caggatcggc aacgcgttcc
     1081 tcttcggcct gcccggaaac ccggtggcgg tgatggtgac cttctaccag ttcgtgcgcg
     1141 atgcgctgct cttcatgtcg ggccgggaat cgggcgagat gccgctcctc agtgcaatcg
     1201 ccgccgaaaa cctgcgcaag gtgccgggcc ggaccgaata tcagcggggc gtcattttca
     1261 aggatcagga tgagtggaaa gtacgcacca ccggccagca gggctcgggc gtgctgcgct
     1321 cgatgtcgga ggcgaactgc ttcatcgtgc tcgagcacga gcgcgcgaac gtcaaggcgg
     1381 gtgagctcgt gagcgtgcag cctttcgagg gacttgcgta gtggcactcg ttgtcctcag
     1441 ctcgctcgcc ctcaaaggcg tgctggagaa atcgagagcg caactggagg aatcgctggg
     1501 cacgcggctc gagctgcact tcgacgccac gcaggcgatc gtgccgcggc tcgccgctgg
     1561 cttccgcgcc gatctggtga tcctcacgcg cgaggcgatc gaggagctgc acacggcggg
     1621 ccgcgtcacg caggttcggg atgtcggcag ctccggcgtc ggcgttgccg tgcgcgccgg
     1681 cgcgcgcaag ccggacatcg gctcggtgga agcgctcaaa catgccctcg tgtcggcgaa
     1741 ctcggtggcg cactccaaag tcggggcgag cggcatctat ttcgtcgagc tactcgagcg
     1801 tctcgatatc agattgaaga agcgcgtggt cgtcgataaa gggccggtcg ggcttgccgt
     1861 ggcgtccggc gaggcggagc tcggcgtgca gcagctttgc gagctcgagc ccgtgccggg
     1921 catcgagatc gtcggcccgc tgcccgatgc ggtgcagaaa atcacgtact tctcggccgg
     1981 tatcccggtg cacgccgcgc acgcggccag cgcgcgcgcc ttgatggagg cgctgcagct
     2041 ctagatcgcc atcacgcgcg cgagctcgag caggctcgcg tccgcgggac gcgtgcgccc
     2101 gtcgatctcg gagagcggcg tggtgacgat gtcgccgcgc acgatgccga ccagcacgcc
     2161 atggcctccg gcggcgagcg tatcgatcgc ggcggcgcca aggcgcgtgg cgagcacgcg
     2221 gtcggcggcg cccggcgtgc cgccgcgcac gacgtggccg aggcgcgtca cgcgaaggtc
     2281 gaacccgatg agggcgcggt ttgtcttgta gtactccatc agctcgtgca cgccgcagcg
     2341 cgccttctcg gcgattaccg cgagcgcgtg cgttttcccg cggcggtacg cgtcgcgcag
     2401 ccgctcggcg atgtgcgccg gcgtgatgtc gacctcggga atcgcaatca cctccgcccc
     2461 gccggcgatg ccggcgatga gtgcgatgta gccgcaatcg cgccccatgg tctccaccgc
     2521 gaaggcgcgc tggtgcgatg acgccgtggt gcgcagccgg tcgatcgcct cgagggtgat
     2581 gttgatcgcg gtgtcgcagc cgatgctcac gtcgttgccg tagagatcgt tgtcgatggt
     2641 ggagggcacc ccgaccaccg cgaagccgtc gcgggtcagc gccgccgagc cggtctgcga
     2701 gccgttgccg ccgatgacga cgagggcttc gacgtcctct ttcttcagat tggcgatcgc
     2761 ttgcctgcgg ccggcgggct cggcgaactc cgggcagcgc gcgctgccga gcacggtgcc
     2821 gcccgactgc atgatgccgc cgacgtcgcg cgccgccagg ggctcgaaat gcccgccgag
     2881 caggccggcg tagccgttgc gcacgccggc cacctgccag ccgcgcgcca gcgccccgcg
     2941 cgtcacggcg cgaatggcag cgttcatgcc cggggcgtcg ccgccgctcg tcaggactgc
     3001 aattttcttc aacatgacga ttctaaaggg ggatgggtaa gcctctggat gaacttcgaa
     3061 cagcggcgcg cggctgccag gactgcccgc tctgggccaa cgcgacgcag acggtgttcg
     3121 gcgcgggcga cccgcacgct cgcgtgatgc tggtaggcga gcagcccggt gacgaggagg
     3181 acaagaaggg attgccgttc gtcgggccgg cgggacgcct cctcgaccgc gcgctcgagg
     3241 cggccggcgt ggaacgcgag cacctgtacg tcaccaacgc ggtgaagcat ttcaagtggc
     3301 agctgcgcgg caagcggcgg ctgcacaaga ccccggcgca gcgcgagatc gaagcctgcc
     3361 accagtggct ggaaagggaa attgaaagca tcgaacccca tgtgatcgtg gcgctgggat
     3421 ccaccgcggc caaggcggtg atcgcgcgcg acttcaaggt ctcggtacag cgtgggcagt
     3481 tcgtcgacag cccgctcgcg ccttacgtgt tcgcaacctt tcatccctcg gccctgctgc
     3541 ggctgcggga agacgaagag cgcgaagtgg ctttcaagca attggtcacg gatctgaaat
     3601 tgattaaaaa agccttagct cgttaactga gcggccatag cagcgcggcc ccgcgtaccc
     3661 cgctcgcgtc gccgtgcagc gcgcgcacga tgggcgtgcg cggttcgcgg tggtagacgt
     3721 acttgcgcag cagcggcgcc acgttggtgt agatgctctc gtgcttcgac atgccgccgc
     3781 cgaccacgat tacgtcgggg tcgaggatgt tgatgacgga gctgaatgcg cgcgccaggc
     3841 ggtcctcgaa gcgcgccatc gccgcctcct cgccgagcag g
//
LOCUS       DASUNT010000473         2833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_854947, whole genome shotgun sequence.
ACCESSION   DASUNT010000473 DASUNT010000000
VERSION     DASUNT010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854947"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2457
                     /locus_tag="VFX94_09555"
     CDS             <1..2457
                     /locus_tag="VFX94_09555"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5768465.1"
                     /translation="HWRRMLGAPMLRGGKPLGALVVAWREPGKAPPRQVELLQTFADQ
                     AAIAIENVRLFNETNEALEQQTAISEILRVISGSPADVQPVLEAVAERAAKICDAVDA
                     QIFLREDNEMRHVARFGTMPIGVELGHARPLNRGWAAGRAVLEARAIHIEDLLAVPRE
                     EYPVSHELRDHAGHRTLLSVPLMRERRALGAISLRRMEVRPFTEKQIALLKTFADQAA
                     IAIENVRLFNETRESLERQTATAEVLKTISRSTFDLDTVLQALLDNAARLGGAEQGVM
                     LRPDAAGNYLPAFAYNWSETVLERLRERPIRPGRDSTNGRVLLEGKPIHIPDVLADPE
                     YGRKDLVSIGGYRAVLSVPMLRDGEAIGLISLSSKSPFTEKQMEVVTSFADQAVIAIE
                     NVRLFNETKEALDQQKASADVLGVISSSIADTTPVFDKILDSCQRLFAGRTVGISVAG
                     DDGRIHLGAYKGSGRAEFEAHFPVPLSMESGSGAAILQRQVVHYPDCEAPDVPEFARV
                     AAMLTGNKSLFFAPMLWEGKGVGAIFVGRPVTGPLTEKEIALLRTFADQAAIAIQNAK
                     LFREIAEKSAQLEVANKHKSEFLANMSHELRTPLNAIIGFSEALIDRLFGELNEKQAE
                     YLKDIHESGRHLLSLINDILDLSKIEAGRMELDVSTFDLPAALSNAITLVRERAQRHA
                     IALALEVDKSLGAFSGDERKFKQIMLNLLSNAVKFTPDGGRVAVSARKDTTHVEISVQ
                     DTGIGIAPEDQAAVFEEFRQVGRDHMRKAEGTGLGLSLVKRFVELHGGEIRLQSEPGK
                     GSTFSFSLPLARAGSSASSA"
     gene            complement(2411..>2833)
                     /locus_tag="VFX94_09560"
     CDS             complement(2411..>2833)
                     /locus_tag="VFX94_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5768466.1"
                     /translation="GAVQERRPDGTPAKANASWKPHPMQGWSPDFVPKLTGDAVDMKV
                     IHKVLTITGPEAMQCAKDLAQKEGIFVGITAGGTLAAALKVCKEARKGSTLLAMLPDT
                     GERYLSTALFSDIPADMTEEELAIMRSTPSCQLAPKAG"
BASE COUNT          469 a          937 c          969 g          458 t
ORIGIN      
        1 cactggcgcc ggatgctcgg ggcgccgatg ctgcgcggcg gcaagccgct cggcgcgctg
       61 gtggtcgcgt ggcgcgagcc gggcaaggcg ccgccgcggc aggtggagct gctgcagacc
      121 ttcgccgacc aggccgcgat cgcgatcgag aacgtgcgcc tgttcaacga aaccaacgag
      181 gcgctcgagc agcagactgc gatcagcgag atcctgcgcg tgatctcggg gtctccggcc
      241 gacgtccagc cggtgctcga ggcggtggcc gagcgcgccg cgaagatctg cgatgccgtc
      301 gatgcccaga tcttcctgcg cgaggacaac gagatgcggc atgtcgcccg cttcggcacc
      361 atgccgatcg gcgtcgagct ggggcatgcg cggccgctca accgtggctg ggccgccggc
      421 cgcgcggtgc tcgaggcaag ggcgatccat atcgaggacc tgctggccgt gccgcgcgag
      481 gaatatcccg tgtcccacga attgcgcgac cacgccggcc atcgcacgct tctctccgtg
      541 ccgctgatgc gcgaacgacg agctcttggc gcaatctcgc tgcgcaggat ggaagtgcgg
      601 ccgttcacgg agaagcagat cgcgctcctc aagaccttcg ccgaccaggc ggcgatcgcc
      661 atcgagaacg tgcgcctgtt caacgagacg cgggagtcgc tcgagcgcca gaccgcgacc
      721 gccgaggtgc tgaagaccat cagccgttcc actttcgact tggataccgt gctgcaggcg
      781 ctgctcgaca acgcggcgcg cctgggcggg gcggaacagg gcgtcatgct ccgacccgat
      841 gcggccggaa actatctgcc ggctttcgcc tacaactgga gcgagacggt gctcgagcgc
      901 ctgcgcgagc ggccgatccg gccggggcgc gattcgacca acggccgggt gctgctcgag
      961 ggcaagccga tccacattcc cgacgtgctc gccgatcccg agtacgggcg caaggacctc
     1021 gtcagcatcg gcgggtatcg cgccgtgctt tcggtgccga tgctgcgcga cggcgaggcg
     1081 atcgggctca tctccctcag cagcaaatcg ccgttcaccg agaagcagat ggaggtggtc
     1141 acgagcttcg ccgatcaggc ggtcatcgcg atcgagaacg tgcgcctgtt caacgagacc
     1201 aaggaggccc tcgaccagca gaaggcgtcg gccgacgtgc tcggcgtgat ctcgagctcg
     1261 atcgcggaca cgacgccggt attcgacaag attctcgaca gctgccagcg cctttttgcc
     1321 gggcgcaccg tcggcatcag cgtcgcaggc gacgacgggc ggatccacct cggcgcctac
     1381 aagggctctg gccgcgccga gttcgaggcc cacttcccgg tgccgctcag catggagtcg
     1441 ggctccggtg ccgcgatcct gcagcgccag gtggtccatt atccggactg cgaggcgccg
     1501 gatgttccgg aattcgcgcg cgtcgccgcc atgctcaccg gcaacaagtc gctgttcttc
     1561 gctccgatgc tgtgggaagg caagggcgta ggggcgattt tcgtcggccg tccggtgacc
     1621 ggccctctta ccgagaagga gatcgcgctc ctcaggacgt tcgccgacca ggcggcgatc
     1681 gcgatccaga acgcgaagct gttccgcgag atcgcggaga agagcgcgca gctcgaggtc
     1741 gcgaacaagc acaagtcgga gttcctcgcc aacatgtcgc acgagcttag gacgccgctc
     1801 aacgcgatca tcggcttcag cgaggcgctg atcgatcggc tcttcggcga gctcaacgag
     1861 aagcaggccg agtatctcaa ggacatccac gagtccgggc ggcacctgct ctcgctcatc
     1921 aacgacatcc tcgatctctc gaagatcgaa gccgggcgca tggagctcga cgtgtcgacc
     1981 ttcgacctgc cggcggcgct ttccaacgcc atcacgctcg tgcgcgagcg cgcgcagcgc
     2041 cacgcgatcg ccctcgcgct cgaggtcgac aagagcctgg gcgcgttctc gggcgacgag
     2101 cgcaagttca agcagatcat gctgaatttg ctctcgaacg cggtgaagtt cacgcccgac
     2161 ggcgggcgtg tcgccgtttc tgcccgaaaa gacactaccc atgtggaaat ctcggtgcag
     2221 gacaccggga tcggcatcgc gccggaggac caggcggcgg tgttcgagga gttcaggcag
     2281 gtggggcgcg accacatgcg caaggccgag ggcacggggc tcggcctctc gctcgtcaag
     2341 cgcttcgtcg agctgcacgg cggcgagatc cgcctgcaga gcgagcccgg caagggctcg
     2401 actttctcct tcagcctgcc tttggcgcga gctggcagct cggcgtcgag cgcatgatgg
     2461 ccagctcttc ctcggtcatg tcggccggga tatcggagaa aagcgcagtg gagaggtagc
     2521 gctcgccggt gtccggcagc atggcgagga gcgtggatcc cttgcgcgcc tccttgcata
     2581 ccttgagcgc cgcggcgagc gtgccgccgg ccgtgatgcc gacgaagatg ccttccttct
     2641 gcgccaggtc cttggcgcat tgcatggcct cgggaccggt gatggtcagc accttgtgga
     2701 tcaccttcat gtcgaccgcg tcgccggtga gcttgggcac gaagtcgggg ctccagccct
     2761 gcattgggtg gggcttccac gaagcgttcg ccttcgccgg cgtgccgtcg ggccggcgct
     2821 cctgcacggc gcc
//
LOCUS       DASUNT010000474         2768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1118103, whole genome shotgun sequence.
ACCESSION   DASUNT010000474 DASUNT010000000
VERSION     DASUNT010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118103"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VFX94_09565"
     CDS             complement(<1..743)
                     /locus_tag="VFX94_09565"
                     /inference="COORDINATES: protein motif:HMM:NF027838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX5768467.1"
                     /translation="MTVFARSFLLIAALIVTAVLASFQIYLQYEREPRSRELAQQTVS
                     TVNLTRAALVSADPFLRRQLLVDLNETEGLRVFPATASEKLEPLPDDALLNLVTRKVR
                     ETTGPETRFAYARDGEEGFLVSFFIDEDEFWVMLPRERFELELGLQWLGWGLALLALA
                     LAGAWLIASNLARPLAALTRAAERIGRGKPHHPIAEEGPRELRTLGSAFNRMARDLES
                     MERERAMVLAGISHDLRSPLSRLRLALEIA"
     gene            complement(740..1474)
                     /gene="ompR"
                     /locus_tag="VFX94_09570"
     CDS             complement(740..1474)
                     /gene="ompR"
                     /locus_tag="VFX94_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HEX5768468.1"
                     /translation="MPDLKTRILVVDDDQRLRELLVRYLGGEGHEVKAVPDAAAMDKQ
                     LARERYDLVVLDLMLPGEDGLAICRRLRAQETAPAIIMLTAKGDDVDRIVGLEMGADD
                     YLPKPFNPRELLARINAVLRRRVPAGPPGAPAAGGVYAFGEFSLNLATRTLTRAGKQV
                     ALTTGEFSVLKVLVQHPRQPLSRDKLMELARGREYEVFDRSIDVQISRLRKIVEEDPS
                     HPKHIQTVWGFGYVFVPDVESPAATQ"
     gene            1571..1822
                     /locus_tag="VFX94_09575"
     CDS             1571..1822
                     /locus_tag="VFX94_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768469.1"
                     /translation="MMLRAALAFVLVVAATTAGAQSGKRPDGRERQMSPEQRERMRDD
                     MRDAYRPRDERPQKTRQMSPEERGQLRRDIEDANRNLKR"
     gene            complement(1845..>2768)
                     /locus_tag="VFX94_09580"
     CDS             complement(1845..>2768)
                     /locus_tag="VFX94_09580"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX5768470.1"
                     /translation="RFRSLTQMSSDFFWETDEAHRFTQLVHGPDYLPPEMSRGVIGKA
                     PWELPNESPDEAGWAAQRARLEEHLPFRDFEFARRMPDGVVRYLSVSGDPRFTPQGAF
                     IGYRGVGRDITEIALARKHIASLAYSDPLTGLANRTSLMPALDQAVQRARRRNAKLAV
                     VFLDLDGFKAINDAYGHDAGDALLVELAGRLRDNLRASDLIARLGGDEFLVVLEEVQD
                     TEPVAVVARKLLAETVRPYTLPGARASVTASIGISIFPDDAADASALMKHADIAMYAA
                     KQAGKNTCRFYSSGPAANDPSQATASSGTQP"
BASE COUNT          444 a          928 c          981 g          415 t
ORIGIN      
        1 gcgatctcga gcgcgaggcg caggcgcgag agcggcgagc gcaggtcgtg cgagatcccg
       61 gcgagcacca tggcgcgctc gcgctccatg ctttccagat cgcgcgccat gcggttgaag
      121 gcggagccga gggtgcgcag ctcgcgcggc ccttcctcgg cgatcggatg gtgcggcttg
      181 ccgcgcccga tacgttccgc ggcgcgcgtc agcgcggcga gcggacgcgc gaggttggag
      241 gcgatgagcc aggcaccggc gagggcgagc gcgagcagcg cgagccccca gccgagccac
      301 tgcaggccga gctcgagctc gaagcgctcg cgcggcagca tgacccagaa ttcatcctcg
      361 tcgatgaaga agctgaccag gaagccttcc tcgccgtcgc gcgcgtaggc gaagcgggtc
      421 tccggtccgg ttgtttcccg cacttttctg gtcaccaggt tcaacagcgc gtcgtcgggc
      481 aggggctcga gcttctcgct ggcggtcgcc gggaacacgc gcaggccttc cgtctcgttc
      541 aggtccacga ggagctggcg ccgcaggaaa gggtccgcgc tcaccagcgc ggcgcgcgtc
      601 aggttcaccg tgctcacggt ctgctgcgcg agctcgcggg agcgcggctc gcgctcgtac
      661 tgcaggtaga tctggaaaga ggcgaggacc gcggtgacga tgagcgccgc gatcagcagg
      721 aacgaccggg cgaagaccgt cactgggtgg cggccgggct ttccacgtcc gggacgaaca
      781 cgtagccgaa gccccacacg gtctggatat gcttcggatg cgaaggatcc tcctccacga
      841 tcttgcgcag gcgcgagatc tgcacgtcga tcgagcggtc gaacacctcg tactcgcgcc
      901 cgcgcgcgag ctccatcagc ttgtcgcgcg aaagcggctg gcgcgggtgc tgcaccagca
      961 ccttgagcac cgagaactcg ccggtcgtga gcgcgacctg cttgccggcg cgcgtcaggg
     1021 tgcgcgtggc aaggttgagc gagaattcgc cgaacgcgta cacgccgccc gcggcgggtg
     1081 cgccgggcgg gccggccggc accctgcgcc gcaggaccgc attgatgcgc gccagaagct
     1141 cgcgcgggtt gaatggcttg ggcaaatagt cgtccgcgcc catctccagg ccgacgatgc
     1201 ggtccacgtc gtcgcccttg gcggtcagca tgatgatcgc gggcgcggtt tcctgcgcac
     1261 gcagcctgcg gcagatggcg agaccatcct ccccgggcag catgaggtcg agcaccacga
     1321 ggtcgtagcg ctcgcgcgcg agctgcttgt ccatggccgc agcgtccggc actgccttga
     1381 cctcgtggcc ctcgccgccg aggtagcgca ctaatagctc gcgcaagcgt tgatcgtcgt
     1441 cgacgaccag aatccgggtc ttcaggtcgg gcataggaaa ggcaaagaag ctttattgaa
     1501 gcttcatcgg gtaatagatt gttacaaaag gacgcccggc cgcaaggcgg gccgcgtaac
     1561 attcgcgttc atgatgctac gcgctgcgct cgctttcgtc ctcgtcgtcg ccgcaacgac
     1621 cgctggcgcg cagtcgggca agcggcctga cggccgcgag cgccagatga gcccggagca
     1681 gcgcgagcgc atgcgcgacg acatgcgcga cgcctaccgc ccgcgcgacg agcggccgca
     1741 aaaaacccgc cagatgtcgc cggaggagcg cggccagctg cgccgcgata tcgaagacgc
     1801 caaccggaac ctgaagcgct agcgagttcg gggacgcagc tcggttaggg ctgcgtccca
     1861 gagctggccg tagcctgcga aggatcgttg gccgccggcc cggacgagta gaagcggcag
     1921 gtattcttgc ccgcctgctt cgcggcgtac atcgcgatgt cggcgtgctt catcaacgcg
     1981 ctcgcgtccg cggcgtcgtc gggaaagatg ctgatgccga tgctcgcggt cacgctcgct
     2041 cgcgcgccgg ggagggtgta ggggcgcacc gtctcggcga gcagcttcct cgccaccacc
     2101 gcgaccggct cggtgtcctg cacttcctcg agcaccacga ggaactcgtc gccgccgagg
     2161 cgcgcgatca ggtcgctcgc ccgcaggttg tcgcgcagcc gcccggccag ctcgacgagc
     2221 agcgcgtcgc ccgcatcgtg gccgtacgcg tcgttgatcg ccttgaagcc gtcgaggtcc
     2281 aggaacacca ccgcgagctt ggcgttccgg cgccgcgcgc gctgcaccgc ctggtcgagc
     2341 gcgggcatga ggctcgtgcg gttggcgagc ccggtcagcg gatcgctgta ggcgagcgag
     2401 gcgatgtgct tgcgcgcgag cgcgatctcg gtgatgtcgc ggcccacgcc gcggtagccg
     2461 atgaacgcgc cttgcggggt gaagcgcggg tcgccgctca cggagagata ccgcaccacg
     2521 ccgtcgggca tgcgccgcgc gaactcgaag tcacggaacg gcaggtgctc ctcgagccgg
     2581 gcacgctgcg ccgcccagcc cgcctcgtca gggctctcgt tcggaagctc ccagggcgcc
     2641 ttgccgatta caccgcggct catttcggga ggcaggtaat cagggccgtg cacgagctgg
     2701 gtgaagcgat gcgcctcgtc agtctcccag aagaagtccg acgacatctg cgtcaggctg
     2761 cgaaagcg
//
LOCUS       DASUNT010000475         3629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1030047, whole genome shotgun sequence.
ACCESSION   DASUNT010000475 DASUNT010000000
VERSION     DASUNT010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030047"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VFX94_09585"
     CDS             complement(<1..688)
                     /locus_tag="VFX94_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5768471.1"
                     /translation="MRLWPIAAAVALAAAPVYAQQKIVKIDGSSTVFPVTEAVAEDFQ
                     KAKKQQVKVTVGISGTGGGFKKFCRAETDVSNASRPILKAEMTDCAKAGVQYIELPVA
                     FDALTVVINPKNGFIKQLTVAEMKKMWEPAAQGKVTRWNQVNPSWPDAPMKLFGPGAD
                     SGTFDYFTEAVVGKSKSSRGDFTASEDDNVLVQGVSRDVNGLGYFGYAYYIENKDKLK
                     AVPIVNEKGEP"
     gene            complement(776..2104)
                     /locus_tag="VFX94_09590"
     CDS             complement(776..2104)
                     /locus_tag="VFX94_09590"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5768472.1"
                     /translation="MPRWLDAAAVYLQRWLELQMRLHEQPGCVVALAHRGRVVCEATF
                     GYADLVTRAPLTPRHRFRVASHSKSFTAAGIMKLQEQKKLRLHDPVGRYVEGLHPALA
                     RATLEQLLSHASGVVRDGPDSGQFTDRRPFLDEAELRAQLKAAPILRRGTRFKYSNHG
                     YGLLGLVIESVTGEPYRAWIAREVVAAAGLAETVPDMPLAKGTPFARGHTGQLPAGRR
                     LVIPGDFPTNAIAAAGGFVSTAGDLARFFSQLSPSAHRSVISIRSRRAMVRRRWRDPH
                     SSIERYYGLGIISGTLAGWSWFGHSGGLQGYITRTAVLPGETSVAVLTNAIDGLAHFW
                     LDGAIHILRTLERHGAPARRPAPWSGRWWNMWNAVDVVPVAAKVLVAMPALSNPFMDA
                     AEIRRGRIALAGGFASHGEPARLGRRELWLGGARYVRETALARELIARYP"
     gene            complement(2104..3264)
                     /locus_tag="VFX94_09595"
     CDS             complement(2104..3264)
                     /locus_tag="VFX94_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HEX5768473.1"
                     /translation="MKTPVAIVGAGPAGLLLSHLLHLDGVDSVVLESRTRQEIEATIR
                     AGVLEQGTVDLLAASGVGERMKREGFVHEGIVLRFANASHRIALTELTGRSIMVYAQH
                     EVIKDLVKARLDAGGKLFFGVKDAALYDLDSASPKVRFHREQSVHEIHAQFVAGCDGF
                     HGVSRPSIPSRHRHEFTRTYPFGWFGILVEAPASTAELIYAHHERGFALVSTRSPQIQ
                     RLYFQCDPEDDLKSWPDTRIWEELHARLATREGWKLTEGRIFQRGIIAMRSFVVEPMR
                     YGRLFLAGDAAHIVPPTGAKGLNLAVNDVRVLARAFAEYFKGGSEDGLNAYSASALKR
                     VWRAEHFSWWMTSMLHRFHDDTPFQHRLQLAELDYVVSSKAKAITLAENYVG"
     gene            complement(3261..>3629)
                     /locus_tag="VFX94_09600"
     CDS             complement(3261..>3629)
                     /locus_tag="VFX94_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX5768474.1"
                     /translation="LFALSFAAATLVPLPSEAALFAYIHMYPDRAALAVAIATAGNTA
                     GGMTSYALGRLIPERKIDLKAVRWLRRYGAPATLLAWLPVVGDALCVAAGWLRIHWAA
                     ALGFMATGRLARYIAVASLA"
BASE COUNT          603 a         1210 c         1230 g          586 t
ORIGIN      
        1 cgggctcgcc cttctcgttc acgatcggca cggccttcag cttgtccttg ttctcgatgt
       61 agtaggcgta gccgaagtag cccaggccgt tcacgtcgcg cgaaacgccc tgaacgagga
      121 cgttgtcgtc ctcggaagcg gtgaagtcgc cgcgcgagga cttggacttg ccgacgacgg
      181 cctcggtgaa atagtcgaac gtgcccgagt cggcgcccgg cccgaaaagc ttcatcggtg
      241 catccggcca cgaggggttg acctggttcc agcgcgtcac tttgccctgg gccgcgggtt
      301 cccacatctt cttcatctcc gcgacggtaa gctgcttgat gaagccgttc ttggggttga
      361 tcaccacggt gagcgcgtcg aaggccaccg gcagctcgat gtactggacg cccgccttgg
      421 cgcagtcggt catctcggcc ttgaggatcg ggcgcgaggc attcgaaacg tcggtctcgg
      481 cgcggcagaa cttcttgaac ccgccgcccg tgccggagat gccgacggtc accttcactt
      541 gctgcttctt cgccttctgg aagtcttcgg ccaccgcctc ggtgaccggg aagacggtgc
      601 tcgagccgtc tatcttgacg atcttctgct gcgcataaac cggtgcggcc gccagtgcaa
      661 cggcggcggc aatgggccat agcctcatgg ggtctcctgt gggatgtgga ctataggccg
      721 gcagggtacg cagtccgcat taccgattta tgaccccggc aagcccgcct tcgtattagg
      781 gatagcgcgc tatcagctcg cgcgcgagcg ccgtctcccg cacgtagcgc gccccgccca
      841 accatagctc cctgcgacca aggcgcgcgg gctcgccatg gctggcgaaa cctccggcga
      901 gcgcgatgcg cccgcggcgg atctccgccg cgtccatgaa cgggttggac aaagctggca
      961 tggcaaccag caccttcgcc gcgaccggca cgacatcgac cgcattccac atgttccacc
     1021 accgtccgct ccagggagcc ggccgccgtg ctggcgcgcc atggcgctca agcgtgcgca
     1081 ggatatggat cgcgccgtcc agccagaaat gggcgaggcc gtcgatcgcg ttggtgagga
     1141 cggccaccga ggtttcgccg ggcagcaccg cggtacgcgt gatgtatccc tgcaggccgc
     1201 ccgaatggcc gaaccagctc cagccagcga gcgtgccgct gatgatccca agcccgtagt
     1261 agcgctcgat gctcgagtgc gggtcgcgcc agcggcggcg aaccatggcc cttcggcttc
     1321 ggatcgaaat gacgcttcgg tgcgccgacg gcgagagctg gctgaaaaac cgcgcgaggt
     1381 cacccgctgt actgacaaag ccgcccgcgg ccgcgatggc gttggtcggg aagtcgcccg
     1441 ggatcacgag ccgccggccc gcgggcagct ggccggtgtg accgcgcgcg aacggcgtgc
     1501 ccttcgcgag cggcatgtcc gggaccgttt ctgcgagccc ggcggctgcc acgacctcgc
     1561 gcgcaatcca ggcacggtat ggctccccag tgacgctttc gatgaccagg ccgagaaggc
     1621 catagccgtg gttggagtat ttgaaccgcg tgccgcggcg caggatcggc gctgccttga
     1681 gctgcgcgcg aagctccgct tcgtcgagaa acggcctgcg gtcagtgaac tgcccggaat
     1741 caggcccgtc gcgcacgacg ccggatgcgt gcgaaagcag ctgctctagc gtcgcgcggg
     1801 cgagcgccgg atgcaggcct tcgacatagc gcccgaccgg atcgtgcagc ctcagtttct
     1861 tctgctcctg caacttcatg attccggctg cggtaaagct cttggaatgc gaggcgacgc
     1921 gaaagcggtg gcgcggggta agcggcgcgc gcgtcaccag gtcggcgtag ccgaacgtcg
     1981 cctcgcaaac gacccggccc cggtgggcca gcgcaacgac gcagcccggc tgctcgtgca
     2041 accgcatctg gagctcgagc cagcgctgca gatagaccgc cgccgcgtcc agccaccggg
     2101 gcatcagccg acgtagttct cggccagcgt gattgccttt gctttcgagc tcaccacgta
     2161 atcgagctcg gcgagctgca gccggtgctg gaacggcgtg tcgtcgtgga aacggtgaag
     2221 catggaggtc atccaccacg agaaatgctc ggcgcgccag acgcgcttca gcgcgctcgc
     2281 cgaataggcg ttcagaccgt cctcactgcc gcccttgaag tactcggcga atgcccgcgc
     2341 gagcacgcgc acgtcgttca cggcgagatt gaggcccttc gcgccggtgg gcgggacgat
     2401 gtgggcagcg tcgccggcga gaaaaagccg gccgtagcgc atcggctcga ccacgaagct
     2461 gcgcatggcg atgatgccgc gctggaagat gcggccttcg gtcagcttcc agccctcgcg
     2521 agtggcgagg cgggcgtgca gctcctccca gatgcgcgtg tcgggccagc tcttcaggtc
     2581 gtcttccggg tcgcactgga agtaaaggcg ctggatctgc ggcgagcgcg tactgacgag
     2641 ggcgaagccg cgctcgtggt gcgcgtagat gagctccgcg gtcgaggcag gcgcttccac
     2701 caggatgccg aaccagccga acgggtaggt gcgcgtgaac tcatgccggt gccgcgacgg
     2761 gatcgacggg cgcgatacgc cgtgaaagcc gtcgcagccg gcgacgaact gcgcgtggat
     2821 ctcgtgcacg ctctgctcgc ggtggaagcg caccttcggc gaggcggagt ccaggtcgta
     2881 gagcgccgcg tccttcacgc cgaagaagag cttgccgccc gcgtcgagcc gcgccttgac
     2941 cagatccttg atcacctcgt gctgcgcgta caccatgatc gagcgcccgg tgagctcggt
     3001 gagcgcgatg cggtggctcg catttgcgaa gcgcaggaca atcccctcgt gcacgaagcc
     3061 ttcgcgcttc atgcgctctc ccactccgct cgccgcgagc aggtccaccg tgccctgctc
     3121 gaggaccccg gcgcgaatgg tggcctcgat ttcctggcgg gtgcgcgact cgagcacgac
     3181 cgagtccact ccgtcgaggt ggagcaggtg cgaaagcaga agtcccgccg gcccggcgcc
     3241 gacgatcgcc acgggtgttt tcatgcgagg ctcgccaccg caatgtagcg cgccaggcgg
     3301 ccggtcgcca tgaagcccaa ggcggcggcc cagtggatcc tcagccagcc cgcggcgacg
     3361 cagagcgcgt cgcccacgac gggcagccag gcgagaagcg tcgccggcgc cccgtagcgg
     3421 cgaagccacc gcacggcctt caggtcgatc tttctctccg gaatcaatct tcccaaagcg
     3481 taggaggtca tgccgccggc ggtgttgccc gcggtggcga tcgcgaccgc cagcgctgcg
     3541 cggtcggggt acatgtggat gtaggcgaag agcgccgctt cggaaggcag cggaacaagg
     3601 gtcgccgcgg cgaagctcaa ggcgaacaa
//
LOCUS       DASUNT010000476         3534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1294593, whole genome shotgun sequence.
ACCESSION   DASUNT010000476 DASUNT010000000
VERSION     DASUNT010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294593"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..2373)
                     /locus_tag="VFX94_09605"
     CDS             complement(121..2373)
                     /locus_tag="VFX94_09605"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEX5768475.1"
                     /translation="MKILTPRKKNPGAMAMRVAMLAAALAAAAPAAAQSSKPKAEADR
                     TIIEAEAIEGVSDLEVSARGNAEIRREDMSIFGESLRYNREFGRAEGEGGVRLQRGPD
                     RFFGPRLYYNTLDDTGEFESPAYLIERDRTARGNAERIEFLGPERYRLINANYTTCRP
                     GQEDWRVEASELELDFEAEEGTAVHPRLRFFDTTVLAAPYAGFPIGERRKSGLLTPYY
                     AQTSQRGFEFGIPYYWNIAPEYDATLTPVVMGKRGYQLKTEARYLGRPYAGNLKVEYL
                     PEDRLTDTSREGVSWQHLHNFPRNVVAQVDYNRVSDDFYFVDLASTVKQVSVGNLPQE
                     AHVTHSGSLGRAPYSLQVRLQGFQTLQDPLAPIVPPYERMPQISFSGAYNDLAGALDA
                     ALPVEYVRFEHPTLVEGTRTSMTPTLAAPAVAPGWFFTPKLGLRYASYDLLLDEPAKT
                     PEVAIPWGSLDTGLVFERESGLFRAATQTLEPRLFYVYVPYRNQDAIPIFDTALADFN
                     YAQLFTENRFVGGDRFGDANQLTAALTTRFLNASGQENFRATIGQRYYFENERVGLTP
                     TSPLRTSTESDILGSIGGRLAANWTFDMTAQYNRVLQRGEKFNTAVSYRPATAKVLNA
                     SYRFQRDVLEQVDFSAQWPLRPGWYGVGRYNYSIRDDKLLEGLAGIEYNAGCWVFRAV
                     GARIQAAGNVSSTAFIFQIEFNGIGALGTDEVVSILKRNVPGYSVTNPADSQFAPPAG
                     RQRLPFEQTY"
     gene            2373..3347
                     /locus_tag="VFX94_09610"
     CDS             2373..3347
                     /locus_tag="VFX94_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5768476.1"
                     /translation="MIHGDARRAALERWLSPRLRIAFTLTPASEDASFRRYWRARLED
                     GTSYVAMDAPPDKEDCRAFVRVAGMLAKAGVHAPRVIAQELDQGFLLLTDLGSRTYLD
                     TLNEVNAGELFADATEALLRWQLATRPGELPPYDEALLRREMVLFPQWYVGRHLKAEL
                     SGPQKERLEALFALLVKSALAQPAVYVHRDYMPRNLMVCEPNPGVLDFQDAVLGPITY
                     DMVSLVRDAFVSWDEERVLDWVVRYWQKAKSAGLPVDRDFGEFWRALEWMGLQRHLKV
                     LGIFARINYRDGKPKYLHDTPRFITYARAVSQRYRELAPLARLLDELE"
     gene            3344..>3534
                     /locus_tag="VFX94_09615"
     CDS             3344..>3534
                     /locus_tag="VFX94_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX5768477.1"
                     /translation="MKAMLLAAGRGERLRPLTDEVPKALVEAAGKPLIAWHLERLARA
                     GFREAVINVSHLGERIIER"
BASE COUNT          578 a         1126 c         1214 g          616 t
ORIGIN      
        1 ggtggcggtg atgacgtcct tgttcaccac cgccacgatc cggtcgaccg gcgtgacgcc
       61 ctgcgcggcg agctccaatg gaaggacgag caaaaggatc gggaagatgc gtttcatctc
      121 tcagtacgtc tgctcgaagg gaagccgctg gcgccctgcg ggcggggcga actgcgagtc
      181 ggcgggattg gtcaccgagt agccggggac gttgcgtttc agtatgctga ccacctcgtc
      241 ggtgccgagc gcgccgatgc cgttgaactc gatctggaaa atgaacgccg tagagctcac
      301 gtttcctgct gcctggatcc gggcaccaac ggcacggaaa acccagcatc cggcgttgta
      361 ctcgatgcct gccaggccct ccagcaactt gtcgtcccgg atcgagtaat tgtagcgccc
      421 gacgccgtac cagccgggcc tgagcggcca ctgggccgaa aagtccacct gctcgagcac
      481 gtcgcgctgg aagcggtagc tcgcattcag gactttggcg gtcgcggggc ggtaactgac
      541 cgcggtattg aacttctcgc cgcgttgcag tacgcggttg tactgcgccg tcatgtcgaa
      601 ggtccagttc gcggcgagcc ggcctccgat cgagccaagg atgtccgact cggtcgaggt
      661 gcgcagcggc gaggtcggcg tcaggccgac gcgctcgttc tcgaagtaat agcgctgccc
      721 gatggtagcg cggaaattct cctggccgga cgcgttcaga aagcgggtgg tcagcgccgc
      781 cgtgagctgg ttggcgtcgc cgaaacgatc gccgccgacg aagcggtttt cggtaaagag
      841 ctgcgcatag ttgaagtcgg cgagcgcggt gtcgaagatc gggatggcgt cctggttgcg
      901 gtagggcacg tacacgtaga aaagccgcgg ctcgagcgtc tgcgtcgccg cgcgaaagag
      961 gcccgattcg cgctcgaaga cgagcccggt gtcgaggctc ccccagggaa tcgccacttc
     1021 gggcgtcttc gccggctcat cgagcaagag gtcatagctc gcgtagcgca gcccgagctt
     1081 cggggtgaag aaccagcccg gcgcgacagc cggcgccgcg agcgtcggcg tcatcgaagt
     1141 gcgcgtgcct tccacgagcg tcggatgttc gaagcgcacg tattcaaccg ggagcgcggc
     1201 gtctaacgcg cccgccaagt cgttgtaggc gccgctgaaa ctgatctgcg gcatgcgttc
     1261 gtagggcggc acgatcggcg cgagcgggtc ctgcagcgtc tggaaaccct gcaggcgcac
     1321 ctgcagcgaa tagggcgcgc gcccgagcga gccgctgtgc gtcacgtgcg cctcctgcgg
     1381 caggttgccg accgaaacct gcttcaccgt gctcgccagg tcaacgaagt agaaatcgtc
     1441 cgacacccgg ttgtagtcga cctgcgccac cacgttgcgg ggaaagttat ggaggtgctg
     1501 ccacgagacg ccttcgcgcg acgtatcggt caggcggtct tccggcaggt attcgacctt
     1561 gaggttgccg gcgtagggac gccccaggta gcgcgcctcg gtcttgagct ggtagccgcg
     1621 cttgcccatg actaccggag taagggtcgc gtcgtactcg ggggcgatgt tccagtaata
     1681 gggaattccg aattcgaagc cgcgctggct ggtctgcgca tagtacggcg tgagcagccc
     1741 gctcttgcgc cgctcgccga tggggaagcc ggcgtaaggc gcggcaagga cggtggtgtc
     1801 gaaaaagcgc agccgcgggt ggactgcggt tccctcttcg gcctcgaagt cgagctcgag
     1861 ctcgctcgcc tcgacgcgcc agtcctcctg gccgggacgg caggtggtgt agttggcgtt
     1921 gatcaggcga tagcgctcgg ggccgagaaa ctcgatgcgc tcggcgttgc cgcgcgccgt
     1981 gcggtcgcgc tcgatgagat aggccgggct ctcgaattct ccggtgtcgt cgagcgtgtt
     2041 gtagtacagg cgcgggccga agaaacggtc cgggccgcgc tgcagccgca cgccgccctc
     2101 gccctcggca cggccgaact cgcggttgta gcgcaggctc tcgccgaaaa tgctcatgtc
     2161 ttcgcggcgg atctccgcgt tgccgcgcgc gctcacctcg aggtcgctca cgccctcgat
     2221 cgcttcggct tcgatgatgg tgcggtccgc ctcggccttc ggcttcgagc tctgcgcggc
     2281 tgccggcgcg gcggcggcaa gcgctgcggc gagcatcgcg acgcgcatcg ccatggcgcc
     2341 ggggttcttt ttccttggtg ttagaatttt caatgataca cggcgatgcg cgccgcgcag
     2401 cgctcgagcg ctggctttcc ccgcggctgc gcattgcgtt cacgctcacg cccgcatcgg
     2461 aggacgcaag cttccggcgc tactggcgcg cgaggctcga agacggcacg agctatgtcg
     2521 cgatggacgc gccacccgac aaggaggact gccgggcgtt cgtgcgtgtg gcgggcatgc
     2581 ttgcgaaagc cggcgtgcat gcgccgcgcg tcatcgctca ggagctcgat cagggattcc
     2641 tgctgctcac ggacctcggc tcgcgcacct acctcgatac gctcaatgaa gtcaacgcgg
     2701 gcgagctctt cgccgatgcc accgaagcgc tgctgcgctg gcagctcgcg acgcggccgg
     2761 gggagctgcc gccgtacgac gaagccctgc tacggcgcga gatggttctt tttccgcagt
     2821 ggtatgtggg ccggcacctg aaggcagagc tgtccgggcc gcaaaaggag cgtctggaag
     2881 cgctcttcgc cctccttgtg aagagcgcgc tcgcgcagcc cgcggtttac gtccaccgtg
     2941 actacatgcc gcgcaacctc atggtttgcg aaccgaaccc cggagtgctc gatttccagg
     3001 acgccgtgct cggcccgatc acctacgaca tggtgtcgct cgtgcgcgac gcattcgtga
     3061 gctgggacga agagcgcgtg ctcgactggg tcgtgcgcta ctggcagaag gcgaaaagcg
     3121 cgggcctgcc ggtggaccgc gacttcggcg agttctggcg cgcgctcgaa tggatgggat
     3181 tgcagcgcca cctcaaggtg ctcggcatct tcgcgcgcat caattatcgc gacggcaagc
     3241 ctaagtacct gcacgacacg ccgcgcttca tcacatacgc gcgcgcggta tcgcaaagat
     3301 accgcgagct cgcaccgctc gcgcggctcc tcgacgagct cgaatgaagg ccatgcttct
     3361 cgccgccggg cgcggcgagc ggctgcggcc gctcaccgac gaagtgccca aggcgctggt
     3421 cgaggcggca ggcaagccgc tcatcgcctg gcacctcgag cgcctggcgc gagccggttt
     3481 ccgcgaggcg gtcatcaacg tatcgcacct gggcgagcgc atcatcgagc gcat
//
LOCUS       DASUNT010000477         2876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1030109, whole genome shotgun sequence.
ACCESSION   DASUNT010000477 DASUNT010000000
VERSION     DASUNT010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030109"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFX94_09620"
     CDS             <1..627
                     /locus_tag="VFX94_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5768478.1"
                     /translation="QSAFFSTGQRCTAASRLIVTEGIHNRFVSALTEKMRGLKVDDAL
                     KDGTDIGPVVDPKQLEQDLHYIELGRKEGARLAAGGNRLERAKQGYYLEPALFVDTTP
                     QMTINREEIFGPVAAVIRVKDYDEALAVANDTPFGLSSGIATTSLKHAEHFKRNSAAG
                     MVMVNLPTAGVDYHVPFGGRKASSYGPREQGRYAVEFYTTVKTSYINP"
     gene            627..1901
                     /locus_tag="VFX94_09625"
     CDS             627..1901
                     /locus_tag="VFX94_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5768479.1"
                     /translation="MELSLQVGKYDIQKLLGKGASGTVYLAKDTFTGKEVALKTIEPE
                     VFRDPQFGPVYRAQFVNEASLAGKLRHPHIVGILDAVVGEDSGHIAMELVTGGDLTKH
                     CSPESLLPVADVLQIGFKCCGALDYAAKEGIVHRDIKPANIMLAGGTDVKIADFGAAF
                     LKKTQIVQTAALGSPYYMSPEQIDGSRPLTFHTDMYSLAVVLYELLTGKKPFVGDNLQ
                     DLMNRILRFEPLAPSAARPELPKSLDAVLLRALRKDPAKRHASWSDFALELSKVGELV
                     LRPGAIPDSEKYVTLKKVSMLSTLADAELWELARAGHWRRVPKGSAIVQEDQSGKSFF
                     FLAKGEAKVTKNRKLLNMMSSGECFGEMGYIGSDSPRRHATVEAMSELLVAEFEPAAL
                     ERMSLGAQLQLTRAMMRNVTDRLALANVRLIR"
     gene            1930..>2876
                     /locus_tag="VFX94_09630"
     CDS             1930..>2876
                     /locus_tag="VFX94_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5768480.1"
                     /translation="MKLLLVFLFFAGQALAQPYPSKPIRLVVGFPPGGAADFVSRAFQ
                     EPLGRALGQPIVVENRPGAGSSIAAEHVAKSAPDGYTVLIASPSSILVNPIITPKAGF
                     QPLKDLIAVSKVSSSPLILAVNPALPAASVRELIAHAKQNPGKLNFATSGNGSAPHLA
                     AVLFVRLAGVDMVHVPYKGGAPAVQSVLAGDTQLSFATPPSVLPLVQAGRLRALATTS
                     RNATPLIPGVPGMGEAGLPDYEIGFWYGFFVPAGTPQDIVRRLFEATSQVLKAPETGI
                     MLAREGTETAGSASPEEFADFLTEDAKLWARLVKESGAKA"
BASE COUNT          506 a          981 c          934 g          455 t
ORIGIN      
        1 cagagcgcgt tcttctccac cgggcagcgc tgcaccgcgg cgagccggct catcgtgacc
       61 gaaggaatac acaacaggtt tgtctcggcg ctgacggaga agatgagagg cctgaaagtc
      121 gatgacgcct tgaaagacgg caccgacatc ggcccggtcg tcgatccgaa gcagctcgag
      181 caggatcttc actacatcga gctcggccgc aaggaaggcg ctcggctcgc cgccgggggc
      241 aaccgcctcg agcgcgcgaa gcagggctac tacctcgagc ccgcgctctt cgtcgacact
      301 acgccgcaga tgaccatcaa ccgggaggaa atcttcggcc cggtcgccgc cgtcatccgg
      361 gtcaaggatt acgacgaggc gctcgcggtg gcgaacgaca cgccgttcgg cctttcctcg
      421 gggatcgcca cgacctcgct caagcacgcc gagcacttca agcgcaactc cgccgccggc
      481 atggtcatgg tcaacctgcc gaccgccggc gtcgactacc acgtgccctt cggcgggcgc
      541 aaggcttcga gctacggacc gcgcgagcag ggccgctacg cagtcgagtt ctacactacg
      601 gtcaagacga gctacatcaa cccgtaatgg agttgtccct ccaggtcggg aaatacgata
      661 tccagaagct gctcggcaag ggcgcttcgg gaaccgtcta tctcgccaag gacaccttca
      721 ccggcaagga agtcgcgctg aagaccatcg agcccgaggt gtttcgcgat ccgcagttcg
      781 gcccggtgta ccgggcgcaa ttcgtgaacg aggcttcgct cgccgggaaa ctgcgccatc
      841 cgcacatcgt cggcatcctc gacgcggtgg tcggcgagga ttccgggcac atcgcgatgg
      901 agctcgtcac gggcggggat ctcacgaagc actgctcgcc ggagagcctg ctgccggtcg
      961 cggacgtgct tcagatcggt ttcaagtgtt gcggcgccct cgactacgcg gccaaggagg
     1021 gcatagtcca ccgcgacatc aagccggcga atatcatgct cgccggcggc accgacgtga
     1081 agatcgccga tttcggcgca gccttcctga agaagacgca gatcgtgcag accgcggcgc
     1141 tcggctcgcc ctactacatg tcgcctgagc agatcgacgg atccagaccg ctcaccttcc
     1201 acaccgacat gtactcgctc gccgtcgtgc tgtacgagct cctcaccggc aagaagccct
     1261 tcgtcggcga caacctgcag gacctcatga accgcatcct caggttcgag ccgctcgcgc
     1321 cgagcgccgc gcgccccgag ctgccgaagt cgctcgacgc ggtgctgctg cgggcgctga
     1381 ggaaggaccc ggccaagcgc cacgcgagct ggagcgactt cgcgctcgag ctctcgaagg
     1441 tgggagagct cgtgctgcgg ccgggcgcga tcccggacag cgagaagtac gtgacgctga
     1501 agaaggtgtc gatgctctcg acgctggcgg atgccgagct ctgggagctc gcccgcgccg
     1561 gtcactggcg gcgcgtgccc aaggggagcg ccatcgtgca ggaggatcag tcgggcaaga
     1621 gcttcttctt tctcgccaag ggcgaggcga aggtcacgaa gaaccgcaag ctcctcaaca
     1681 tgatgagcag cggggagtgc ttcggcgaga tgggctacat cggcagcgac agcccgcggc
     1741 gccacgctac cgtcgaggcg atgagcgagc tgctcgtcgc agagttcgag cccgccgcgc
     1801 tcgagaggat gagcctcggc gcgcagctcc agctcacgcg cgcgatgatg cgaaacgtta
     1861 ccgaccggct tgccctcgca aacgtccggc tcatccgcta ggccgactca cgaactcgcc
     1921 gcggccatca tgaaacttct gcttgtcttc ctcttcttcg cgggccaggc gctcgcgcag
     1981 ccttatcctt cgaagccgat ccggctcgtg gtcggcttcc cgcccggcgg cgcggccgat
     2041 ttcgtctcgc gcgcgttcca ggagccgctc ggccgggcgc tcggccagcc gatcgtggtg
     2101 gagaaccgcc cgggcgcggg ctcgagcatc gcggccgagc acgtcgcgaa gagcgcgccc
     2161 gacggctata ccgtgttgat cgcgagcccg tccagcattc tcgtcaaccc catcatcacg
     2221 cccaaggcgg gctttcagcc cttgaaagac ctcatcgcgg taagcaaggt gagctcatca
     2281 ccgctcatcc ttgccgtgaa cccggcgctg ccggccgcat cggtgaggga gctgatcgcc
     2341 catgcgaagc aaaacccggg caagctcaac ttcgcgacct cgggcaacgg ctcggcgccg
     2401 catctcgcgg cagtgctctt cgttcggctc gccggcgtcg acatggtgca cgtcccttac
     2461 aagggcggcg cgcccgcggt gcagtcggtg ctcgcgggcg acacgcagct ttctttcgcc
     2521 acgccgccgt cggtattgcc gctggtgcag gcgggcaggc tgcgtgcgct cgcgacgacc
     2581 agccgcaacg ccacgccgct cattcccggt gtgccgggga tgggagaggc ggggctgccg
     2641 gactacgaga tcgggttctg gtacggcttc ttcgttcccg cgggcacgcc gcaggacatc
     2701 gtgcgccggc tcttcgaggc cacaagccag gtcctgaaag ccccggagac cggcatcatg
     2761 ctcgcccgcg aaggcaccga gaccgcgggc tcggcctcgc ccgaggagtt cgcggatttc
     2821 ctcaccgagg atgcgaagct gtgggcgcgc ctcgtgaagg agtccggtgc caaagc
//
LOCUS       DASUNT010000478         3190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1030433, whole genome shotgun sequence.
ACCESSION   DASUNT010000478 DASUNT010000000
VERSION     DASUNT010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030433"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VFX94_09635"
     CDS             <1..594
                     /locus_tag="VFX94_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768481.1"
                     /translation="DFRAPPPPPYGSDAFRAQVRHVREVSDTRTNSQVRIAQYWENLT
                     GSFAAGAWNAVARSAMVARGFDEAAMARTLAMMHMVAFDAVLACHDSKFAYWVPRPTQ
                     VDPQITLPIGVPNFPAYPSNHACISGAVGRVLDAVLPNTNGVYEAMGRQAGESRIYAG
                     IHYQMDLDAGYEIARKVAARAVEVGVPKDKPFVPMGR"
     gene            675..1025
                     /locus_tag="VFX94_09640"
     CDS             675..1025
                     /locus_tag="VFX94_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768482.1"
                     /translation="MDDLARLVNGDPAIVRWGRGMNETFMVEVGGEQYLLKVSEGKIA
                     IEKGPFVMRPWKFAIRASRESWEKFWQKPPPPGWHDLFALLRRGEVKFEGDQRVLMAY
                     LLYVKLVLAAPRNA"
     gene            1022..1849
                     /locus_tag="VFX94_09645"
     CDS             1022..1849
                     /locus_tag="VFX94_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5768483.1"
                     /translation="MRLEPIVGRYAHIDNCRIYFEEAGQGIPLLCLHTAGSDGRQWRA
                     VLSDAEITRHFRVIAFDMPWHGKSSPPAGWQNEDYRLTTASYTGLVMSVARALGLERP
                     VVMGCSIGGRIVLELALKHASELRALIGLQSGAYVERYYDPSWLDHPEVHGGRACGAV
                     AYGLMSPLSPEKERWETVWHYMQGGPGVFTGDLYFYQVDGDVRERIGAIDTSMCPLYL
                     LTGEYDYSASPRDTEAVVARVKGAKMTVMKGLGHFPMSENPEAFLGYLRPVLKEIGT"
     gene            1846..>3190
                     /locus_tag="VFX94_09650"
     CDS             1846..>3190
                     /locus_tag="VFX94_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX5768484.1"
                     /translation="MKRLLATLAAAASLGHAHADDALLEAMARPWTGDLDGMIERRAV
                     RALVAPSRMQYWIDRGRQSGAEYELLRKFEEVLNNEYKSKRKHIHINVHFIPTARDEL
                     IPALLQGRGDIAAGILTVTPERLRQVDFGAPFFRNVAEIVVTGPASPQLASIEELAGH
                     KLFVRRSSSYWTHLEELNARLGQQGRAPFELKAAPEDLQDDDLLEMLNAGLVGLAVVD
                     RYKATTWARVFKRIRPRDDLKVHEGGDIAWMIRKESPRLKAEISAFAEKHGQDSAFGK
                     NLVKKYTGSTRFVRNATSKEEMRKFDQTVEIFRKYSVTYDMDYLLMMAQGYQESRLDQ
                     NAKSPVGAIGVMQVMPDTGQDMKTGDITQLEPNIHAGVKYIRYVQNTFFESQPMDAVN
                     KTLFSFAAYNAGPGRVQDLRARAEKRGLNPNVWFNNVELIAAERIGAETVTYVSSI"
BASE COUNT          577 a         1013 c         1088 g          512 t
ORIGIN      
        1 gacttccgcg cgccgccgcc gccgccgtac ggctcagacg ccttccgcgc tcaggtccgc
       61 catgtccgcg aggtgtcgga cacgcgcacc aattcgcagg tgcgcatcgc gcagtactgg
      121 gagaacctca ccggctcgtt cgccgcgggc gcatggaatg cagtggcccg ctccgcgatg
      181 gtcgcgcgcg gcttcgacga ggccgccatg gcgcgcacgc tggcgatgat gcacatggtg
      241 gcgttcgacg cggtgcttgc gtgccacgac tccaagttcg cgtactgggt gccgcggccc
      301 acgcaggtgg acccacagat cacgctgccg atcggcgtgc ccaatttccc ggcgtatccc
      361 agcaaccacg cctgcatctc gggcgccgtg ggtcgggtgc tcgatgcggt cctgccgaac
      421 accaacggcg tttacgaggc gatgggccga caggccggcg agtcccgcat ttatgccggc
      481 atccactacc agatggacct ggacgcgggc tacgagatcg cgcgcaaggt ggcggcgcgc
      541 gccgtcgagg tgggcgtgcc gaaggacaag ccgttcgtgc ctatggggcg ctagcgcgcc
      601 tcggccgcgc acgtaattag ctgaattcgg gctctgaccc cgattatcgg gtcagcggga
      661 gggaggaaga cgcgatggac gatcttgccc gactcgtgaa cggggatccg gcgatcgtgc
      721 gctggggacg gggcatgaac gagacgttca tggtggaagt gggcggcgag caatatcttc
      781 tgaaggtatc cgaaggaaag attgctatcg agaaaggccc gttcgtgatg cggccctgga
      841 agttcgccat tcgcgcaagc cgcgaatcgt gggagaagtt ctggcaaaag ccgccgccac
      901 ccgggtggca tgatctcttc gcgctgctca ggcgcggcga ggtgaaattc gaaggcgatc
      961 agcgcgtgct gatggcgtat ctgctctatg tgaagctcgt ccttgccgcg ccgaggaatg
     1021 catgaggctc gagccgatcg tcgggcgtta cgcccacatc gataactgcc gcatttactt
     1081 cgaggaagcg gggcagggga ttccgctcct gtgcctgcac actgccggct cggacggccg
     1141 acagtggcgc gcggtgctga gcgacgcgga aatcacccgg cactttcgcg tcattgcatt
     1201 cgacatgcca tggcatggca agtcatcgcc gcccgccgga tggcagaacg aggactaccg
     1261 gctgaccacc gcgtcgtaca ccgggctcgt catgagcgtg gcccgcgcgc tcgggctcga
     1321 gcgcccggtg gtgatgggct gctcgatcgg cgggcgtatc gtgctggagc tcgcgctgaa
     1381 gcacgcgagc gagctgcgcg cgctgatcgg gttgcagagc ggcgcctacg tggagcgcta
     1441 ctacgacccc tcctggctcg accatcccga ggtccatggc ggccgcgcgt gcggcgcggt
     1501 ggcctacggg ctcatgtcgc cgctcagccc cgagaaggag cgctgggaaa cggtgtggca
     1561 ctacatgcaa ggcggaccgg gcgtcttcac cggcgatctg tacttctacc aggtggatgg
     1621 cgatgtccgc gagcgcatcg gcgcgatcga caccagcatg tgcccgctct acttgcttac
     1681 cggcgagtac gattactctg catcgccgcg tgacaccgag gcggtcgttg cccgagtgaa
     1741 gggagcgaag atgacggtaa tgaaaggtct gggtcacttt cccatgagcg agaaccccga
     1801 agcgttcctc ggctacctgc gcccggtgct caaggagatc ggcacatgaa acgcctgctc
     1861 gcgacgctgg cggcggcggc cagcctcggc cacgcccacg ccgatgacgc gctgctggag
     1921 gcgatggcgc ggccgtggac cggcgacctc gacggcatga tcgagcggcg tgcggtgcgc
     1981 gcgctggtgg cgccttcgcg catgcagtac tggatcgacc gcggccgcca gagcggcgcc
     2041 gagtacgagc tgctcaggaa gttcgaggaa gtgctgaaca acgagtacaa gtccaagcgc
     2101 aagcacatcc acattaacgt gcatttcatc ccgacggcgc gcgacgagct catccctgcg
     2161 ctcctgcagg gccgcggcga catcgccgcc ggcatcctca ccgtcacgcc ggagcggctg
     2221 aggcaggtgg acttcggcgc gccgttcttc cgcaacgtcg ccgagattgt ggtgaccggc
     2281 ccggcctccc cgcagctcgc atcgatcgag gagctcgccg ggcacaagct cttcgtgcgt
     2341 cgctcgtcga gctactggac gcacctcgag gagctgaatg cgcgcctggg tcagcagggc
     2401 cgcgcaccgt tcgagctgaa ggctgcgccc gaggacctgc aggacgacga cctgctcgag
     2461 atgctgaacg ccggcctggt cgggctcgcg gtggtggatc gatacaaggc caccacatgg
     2521 gcgcgcgtgt tcaagcgcat caggccgcgc gatgacctga aggtgcacga aggcggcgac
     2581 atcgcctgga tgatccggaa ggagagcccg cgcctcaaag cggaaatctc cgcgttcgcc
     2641 gaaaagcacg ggcaggacag cgccttcggc aagaacctgg tcaagaagta caccggcagc
     2701 acgcgcttcg ttcgcaacgc gacgtccaag gaggagatgc gcaagtttga ccagacggtg
     2761 gagatcttcc gcaagtacag cgtcacgtac gacatggact atctcctgat gatggcgcag
     2821 ggctaccagg agtcgcgcct cgaccagaac gcgaagagcc cggtcggcgc catcggcgtc
     2881 atgcaggtga tgcccgacac cggccaggac atgaagaccg gcgacatcac gcagcttgag
     2941 ccgaacatcc acgccggggt gaagtacatc cgctatgttc agaacacgtt cttcgagagc
     3001 cagccgatgg acgcggtcaa caagacgctg ttctcgtttg cggcctacaa cgccgggccg
     3061 ggccgggtgc aggacctgcg cgctcgcgcc gagaagcgcg ggctgaatcc gaacgtctgg
     3121 ttcaacaacg tcgagctgat cgccgcagag cggatcggcg cggagaccgt gacctacgtt
     3181 tcgagcatct
//
LOCUS       DASUNT010000479         4056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_680285, whole genome shotgun sequence.
ACCESSION   DASUNT010000479 DASUNT010000000
VERSION     DASUNT010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680285"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFX94_09655"
     CDS             <1..200
                     /locus_tag="VFX94_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768485.1"
                     /translation="LRDAVVDNGRALELANVRFRIGSGDLRAVQQKSLALHAARTALL
                     RVRVERLVQRVNLHLALGGSF"
     gene            complement(171..2102)
                     /locus_tag="VFX94_09660"
     CDS             complement(171..2102)
                     /locus_tag="VFX94_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5768486.1"
                     /translation="MDLLAFECLLAEIASRFADVPPAAVTAEIRAALSRLVDLFGYER
                     CTYSEFSVDGTLNIVASAATPGVKALRLGPFREGLDWFLAELRAGRPLVFPELPNGLP
                     PHATAEAEFVKQHGLRAHLSIPVRMRGRTSGVLSFAGLRKVRDWPKSLIVRVTIIAEV
                     FASAMARARAEEEAHHLRSRLWHADRVAQVSALTAALAHDINQPLTAILSNAQAGLAN
                     LERGEAPPEEMRAILEAVVRDNKRAADTIRTMRAFLRQEEKGHEPIDLGAALHEVLGL
                     LATELGRKAIHIEARLQPGCWVSADKTQIEQVAVNLILNAAQAVEGCPPDERLISLHA
                     GPTGDGRVAVEVRDAGAGIEAQDLQAVFRPFWTTRKKGLGLGLAICRSIVEAHGGEIR
                     AQPNAERGVTFRFELPAEIDSTGQPARAAEQAQLHQASAQPEMHSVGPVVCVIDDDHA
                     VRESLARLLAAQGWPTKSYASAKDFLERQPLAAVGCILLDYQMPGMSGLELQQHLASY
                     GESPPVVFLTGRGDVAAGVQAMKLGAVDFLIKPPDASLLCEAVRKALARQASEKARTR
                     ERAESLHRLGRLSAREREVMAQVVRGRLNKQIAADLDIALQTVKQHRGRVMEKLEVRS
                     VAELVQVYSTLKAAAERQM"
     gene            complement(2219..2593)
                     /locus_tag="VFX94_09665"
     CDS             complement(2219..2593)
                     /locus_tag="VFX94_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5768487.1"
                     /translation="MARALYIVETDVSVREGLTRLAASAGFEAKPCASAEAFLLEVAS
                     GTAACALVDISDAGLRDATLRARLTVLALHLPIIALSCRDDPQAQRTARELGARAFFR
                     KPVDAAALLDSIHWVMRSDSPA"
     gene            complement(2787..3602)
                     /locus_tag="VFX94_09670"
     CDS             complement(2787..3602)
                     /locus_tag="VFX94_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768488.1"
                     /translation="MRYTVHTLLGIALICAATAAAAAEGDIPDHPVLSAKWYFGAGLF
                     IPKTTTEARLTSSTLGAGAIIDFEDSLGVDDEKEVPGFMARWRISERWRIEAEYFQLN
                     RGATRTIDRQIEWGGAVFPINAQVSSNFDFSDLRISVGYSFFRTKDKELGVGLGFHVA
                     EYNVGLSSATTGAQSEDVTAPLPVFNIYGTFALTDRWAVSARLDRLSLNYDKYEGSIG
                     ALGVDLMYQPFRNFGLGIGFRSMLFDLTVEEEAAEFQARQTFSGPILFLTANF"
BASE COUNT          649 a         1377 c         1352 g          678 t
ORIGIN      
        1 tcctgcgcga cgcggtggtc gacaacggcc gcgcgctcga gctcgccaac gtgcgcttcc
       61 gcatcggctc cggcgacctg cgcgcggtgc agcagaagag cctggcgctg cacgccgcgc
      121 gcaccgcgct gctgcgcgtc cgggtcgagc gcctggtgca gcgcgtgaac ctacatctgg
      181 cgctcggcgg cagcttttag ggtcgagtag acctgcacca gctccgcgac cgagcgcacc
      241 tcgagctttt ccatgacgcg gccgcggtgc tgcttgaccg tctgcagcgc gatgtcgagg
      301 tcggcggcga tctgcttgtt gagccggccg cgcaccacct gggccatcac ctcgcgctcg
      361 cgcgccgaca gccggccgag gcggtgcaac gactccgcgc gctcgcgcgt gcgtgctttc
      421 tcgcttgcct gacgtgcgag cgccttgcgc accgcctcgc acagcaggct cgcgtcgggc
      481 ggcttgatga ggaagtccac ggcgccgagc ttcatcgcct gcacaccggc cgcgacgtcg
      541 ccgcggccgg tgaggaacac caccggcggc gattcgccgt agctcgcaag gtgctgctgg
      601 agctccagcc ccgacatgcc cggcatctgg taatcgagca ggatgcagcc gaccgcggcg
      661 agcggctggc gctcgaggaa gtcctttgcg ctcgcatagc ttttcgtggg ccacccctgc
      721 gccgcgagca gccgcgctag gctctcgcgc accgcgtggt catcgtcgat gacgcacacg
      781 accggcccga cggaatgcat ctcgggttgc gcagaagcct gatgcagctg cgcctgctcc
      841 gccgcacgcg ccggctgccc ggtgctgtcg atctcggccg gaagctcgaa ccggaacgtg
      901 acgccgcgct ccgcattggg ctgcgcgcgg atttcgccgc cgtgcgcctc cacgatggag
      961 cggcagatcg caagcccgag gcccaggccc ttcttgcgcg tggtccagaa cggcctgaat
     1021 accgcctgca ggtcctgcgc ttcgatgccg gcgcccgcgt cgcgcacctc cacggcgaca
     1081 cgcccgtcgc ccgtaggccc ggcgtgcagg ctgatcagcc gctcgtcggg cgggcagcct
     1141 tccaccgctt gcgccgcgtt caggatgaga ttcaccgcga cctgctcgat ctgcgtcttg
     1201 tccgcgctca cccagcatcc cggctgcagg cgcgcttcga tgtggatcgc cttgcggccg
     1261 agctccgtgg cgagcagccc gagaacctca tgcagcgccg cgccgaggtc gatgggctcg
     1321 tgcccctttt cctcctggcg caggaaagcg cgcatcgtcc tgatggtgtc ggcggcgcgc
     1381 ttgttgtcgc gtaccacggc ttcgaggatg gcgcgcatct cctccggcgg cgcttcgccg
     1441 cgctcgaggt tcgcaagccc ggcctgcgcg ttgctcagga tcgcggtaag cggctgattg
     1501 atgtcgtgcg cgagggccgc ggtgagcgcg ctcacctgtg ccaccctgtc ggcgtgccac
     1561 agccggctgc gcagatggtg cgcttcctcc tcggcgcgcg cgcgcgccat cgcgctcgcg
     1621 aatacctcgg cgatgatggt gacccgcacg atcaggctct tgggccagtc gcgcaccttt
     1681 cgcaagcccg cgaaagacag cacgccgctc gtgcggccgc gcatgcgcac cgggatggaa
     1741 aggtgcgcgc gcagcccatg ctgcttcacg aactcggctt cggccgtggc gtgcggcggc
     1801 agaccgttcg gcagctcggg gaacaccagc ggccggccgg cgcgcagctc ggcgaggaac
     1861 cagtcgagac cttcccggaa cggccccaga cgcagcgcct tgacgcccgg cgttgcagcc
     1921 gaggcaacga tgttcagcgt accgtcgacg gagaactcgc tgtaggtgca gcgttcgtag
     1981 ccaaataaat cgaccagccg gctgagcgcc gcgcggatct ccgccgttac ggcggcgggc
     2041 gggacgtcgg cgaagcgcga ggcgatctcg gcgagcaggc actcgaaggc gaggaggtcc
     2101 atcggggcaa gctcggcccc ctcgcgaacc tgcccccagc ctgtattgcg ccgccgcatt
     2161 gcgcaaacct aaccgcaagt gaacccgagc gcaacgtccg ggccttgact tggcttaact
     2221 aggcggggct gtcactccgc atcacccagt ggatggaatc aagcagggca gccgcatcga
     2281 ccggcttgcg gaagaaggcg cgcgccccga gctcccgggc ggtacgctgc gcctgcggat
     2341 cgtcccggca cgacagcgcg atgataggca ggtgcagcgc gagaaccgtc agacgcgcgc
     2401 gaagcgtggc gtcgcgcaag cctgcgtccg aaatgtcgac cagcgcgcaa gccgccgttc
     2461 ccgaagctac ctcgaggagg aaagcttcgg cggaagcaca cggcttcgct tcaaagccgg
     2521 ccgacgcggc aagccgagtc agcccctcgc gcaccgacac gtctgtttcg acgatgtaca
     2581 aggcgcgcgc cacggtgtgc ccgttcttgg cgcggatcga atgcatggtc gccgggatgc
     2641 agcgaccaca ttacgcacgc ttcgcgggcc gcgaaatcct accttagtgg tatggcgcgt
     2701 tcttggcgca gcgggccggc ctatttcgtg gtctcgaccg tgctgtacat tccgacggta
     2761 gcccggcagc gactccgcgc gctaaatcag aagttggcgg tgaggaagag gatgggaccc
     2821 gagaacgttt gtctggcctg gaactcggcc gcctcctcct ctaccgtgag atcgaacagc
     2881 attgagcgga agccgatgcc caatccgaag tttcggaacg gctggtacat gaggtcgacc
     2941 ccgagtgcgc cgatgctgcc ttcgtacttg tcgtaattca gcgaaagccg gtcgaggcgc
     3001 gcactcaccg cccagcggtc ggtcagggcg aatgttccgt aaatgttgaa caccggcagc
     3061 ggcgccgtga catcctccga ttgcgcgccg gtcgtggccg agctcaggcc gacgttgtac
     3121 tcggcgacgt gaaaaccgag gcccacgccg agctccttgt ccttggtacg gaagaacgaa
     3181 tagccgacgg agatgcgaag gtcggaaaaa tcgaagttgc tcgacacctg cgcattgatc
     3241 gggaagacgg cgccgcccca ctcgatctgg cgatcgatgg tccgcgtggc gccgcgattg
     3301 agctggaaat actcggcctc gatgcgccag cgctcgctga ttctccagcg cgccatgaag
     3361 cccggcactt ccttttcgtc atcgacgccg aggctatcct cgaagtcgat gatcgcgccg
     3421 gcaccgagcg tgctcgaagt gaggcgcgcc tcggtggtgg ttttcgggat gaagagcccg
     3481 gcgccgaagt accatttggc cgagagcaca gggtgatcgg gaatgtcgcc ttctgctgcg
     3541 gccgctgccg tggcggcgca gatgagtgcg atgccgagca atgtgtgtac ggtgtatcgc
     3601 ataggtgcgc cctcaaaaaa accatgctag tcgaaaggcg catggcatgc ctatagatcg
     3661 cgagttgctg ttccctcgcc gccgttatgc taccgcagtc gtctgccggt tgatgggcgt
     3721 tttgcgcccg cctcgaggat gctttcgcta ccgtcgtgga cccgcgactt ggaggctttc
     3781 atagagcgcc ctacctttgc gggtggccgt accctacaga aggaaggtgt actgcggcga
     3841 gaaagcccgg gcagccgggc tgagaagccc gcccattatc gtcgcagtcc ccctgcgctc
     3901 gagagagaat aagacatgta cctcacccgc ctggtgatcg cgctcgctgc cgctctgccc
     3961 cttgccgccg gcgcgcagac tctgcgctcg gaggagcact cgttccgcgt cgtgaagctg
     4021 gtcgaagggc tcgagcagcc atgggccctc gcgttc
//
LOCUS       DASUNT010000480         2915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_155424, whole genome shotgun sequence.
ACCESSION   DASUNT010000480 DASUNT010000000
VERSION     DASUNT010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155424"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /locus_tag="VFX94_09675"
     CDS             <1..1421
                     /locus_tag="VFX94_09675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5768489.1"
                     /translation="TIHASGKHLLALINDILDLSKVEAGKLEVERIACSPHVIVRQAL
                     LELEVKAREKGIGLEFAATGPVPQSVRSDPLRLRQIVLNLANNAIKFTDRGAVRIEAS
                     LLAGRPARYSVKVIDTGIGIPPDKIGSLFEAFAQADTSITRRYGGTGLGLAISRKLAR
                     ALGGDVTVESEPGKGSLFTVTFETGPLEGVPILSPAEVREDHGESAIVAAARWSIPSA
                     RVLVVDDAAENRELVSLVLAQNGLWVEEADDGRVAVEMALKGGFDVIFMDMQMPVMDG
                     YSATRAIRAAGLKIPIVALTANAMRGHEVQMKKAGCDLFLTKPIDIDELVSAVASLLG
                     GRKLDPTAPAPRPASSPAAAQAGSAVVLTAPAEPPITSRLAGDKRLVPIVHKFLRRLQ
                     ERLAEVRQLEARSDYREIADFAHWLRGSAGSMGYDAFTTPAAELEAAAKDAKAEAVAT
                     LLNEVREMARRVVAPDETSFAA"
     gene            1442..2545
                     /locus_tag="VFX94_09680"
     CDS             1442..2545
                     /locus_tag="VFX94_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5768490.1"
                     /translation="MSAMTETSPARRAVRDIALGELSDALVVIIDDEPLVIELTEAFL
                     QGAGYRRFASSTDPASAIASLLRERPDVVLLDINMPKVSGFDLLARMQADPVLRHIPA
                     IVLTSADDADTKLKALELGASDFLRKPVDPSELALRVRNTLAANAHREALRKTFARYI
                     SPRLAERILDGSAAPFASPPQRADVVALFADLRGFTSITESIDVQEVVRMLNEFFSAL
                     TEATYRHEGTIFNMAGDSLLLGFNVPFPQPDAAERAWRTAAEVLSLVGDAAARWRVRH
                     GISTGVGIGICLGHAVIGNVGSPHYMSYTMIGNAVNAAARLMQIAQAGEALVSGEFYA
                     SIRHLVPPTRVESLGLVTLRGRSEPMPVFSIRA"
     gene            complement(2542..>2915)
                     /locus_tag="VFX94_09685"
     CDS             complement(2542..>2915)
                     /locus_tag="VFX94_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5768491.1"
                     /translation="LLERVGIPEQAEKYPGQLSGGQQQRVAIARALAMEPKIMLFDEP
                     TSALDPEMIKEVLDVMQELARSGMTMIVVTHEMGFARTVADRVVFMDGGRIVEAAPPA
                     QFFGAPKTDRAKLFLSQVLGH"
BASE COUNT          479 a         1011 c          971 g          454 t
ORIGIN      
        1 acacgatcca cgcgagcggc aagcacctcc tcgcgctcat caacgacatc ctggacctgt
       61 caaaggtcga ggccggcaag ctcgaggtcg agcgcatcgc ctgctcgccg catgtcatcg
      121 tgcgccaggc gctgctcgag ctcgaggtca aagcgcgcga gaagggtatc ggcctggagt
      181 tcgccgccac cgggccggtt ccgcagagcg tgcgcagcga cccgctgcgg ctgcgccaga
      241 tcgtcctcaa cctcgccaac aacgcgatca agttcaccga tcgcggcgca gtgcggattg
      301 aagcgtctct ccttgccgga aggcccgcgc gctattcggt gaaggtgatc gacaccggca
      361 tcggcatccc gcccgacaag atcggctcgc tgttcgaggc cttcgcccag gcggacacct
      421 cgatcacgcg cagatacggc ggcacgggcc tcggcctcgc catcagccgc aagctcgcgc
      481 gcgcgctggg cggcgacgtg accgtcgaga gcgagccggg caagggcagc ctcttcaccg
      541 taacgttcga gaccggcccg ctcgaaggcg tgccgatcct ctccccggcc gaggtacgcg
      601 aagaccacgg cgaaagcgcg atcgtggcgg cggcgcgctg gagcattccg tcggcccgcg
      661 tgctagtggt cgacgacgcg gcggagaacc gcgagctcgt ctcgctcgtg ctcgcccaga
      721 acggcctgtg ggtcgaggag gcggacgacg gccgcgtcgc ggtggagatg gcactgaagg
      781 gcggattcga cgtcatcttc atggacatgc agatgcccgt gatggacggc tacagcgcca
      841 cgcgcgcgat tcgcgccgcc gggctcaaga tcccgatcgt cgcgctcacc gcaaacgcga
      901 tgcgcgggca cgaggtgcag atgaagaagg cgggatgcga cctgttcctc accaagccga
      961 tcgacatcga cgagctggtg agcgcggtcg cctcgctgct cggcgggcgc aagctcgatc
     1021 cgacggcccc ggcgccgagg ccggcaagtt ccccggccgc ggcgcaagca ggttctgcgg
     1081 tcgtcttgac ggcgcccgcg gaaccgccga taacgtcgcg cctcgccggg gacaagcgcc
     1141 tggttccgat cgtgcacaag ttcctgcgca ggctgcagga gcgcctggcc gaggtgcgcc
     1201 agctggaagc gcgcagcgat tatcgcgaga ttgccgattt tgcccactgg ctgcgcggct
     1261 cggccggctc aatgggatac gacgcgttca ccacgccggc ggccgagctc gaagccgcgg
     1321 ccaaggacgc gaaggcggag gcggtggcga cgctcctgaa cgaagtacgc gaaatggccc
     1381 ggcgtgtcgt cgcgccggat gaaacgtcgt tcgccgcatg agcacgcatg atgatgcgct
     1441 gatgagcgca atgacagaga cgtccccggc caggcgcgcc gtgcgcgata tagcgctcgg
     1501 cgagctaagc gacgcgctgg tggtgatcat cgacgacgag ccgctcgtga tcgagctcac
     1561 cgaggcgttc ctccaggggg ccggctaccg gcgcttcgcc tcgagcacgg atcccgcgtc
     1621 cgcgatcgca tcgctcctgc gcgagcgccc ggacgtggtg ctgctggaca tcaacatgcc
     1681 gaaagtcagc ggcttcgacc tgctcgcaag gatgcaggcc gacccggtgc tcaggcacat
     1741 tccggcgatc gtgcttactt cggccgacga cgcggacacg aagttgaagg cgctcgagct
     1801 cggggcgagc gatttcctgc gcaagccggt ggacccgagc gagctggcgc tgcgcgtgcg
     1861 caacactctg gccgcgaatg cgcaccgcga agcgctgcgc aagaccttcg cacgctacat
     1921 ttcgccgcgc ctcgccgagc gcatcctcga cggctcggcc gcgccgttcg cctcgccgcc
     1981 ccagcgggcc gacgtcgttg cgctctttgc cgacctgcgc ggcttcacca gcatcaccga
     2041 gtcgatcgac gtgcaggaag tggtgcgcat gctgaacgag ttcttctcgg ctctcaccga
     2101 ggcgacctac cggcacgagg ggacgatctt caacatggca ggcgacagcc tgcttctcgg
     2161 gttcaacgtg ccgtttccgc agcccgatgc ggccgagcgc gcctggcgca ccgccgcgga
     2221 ggtcctttcg ctcgtgggcg acgcagccgc acgctggcgc gtccgccacg gcatctccac
     2281 cggcgtgggc atcggcatct gcctcgggca cgcggtgatc ggtaacgtgg gctccccgca
     2341 ctacatgagc tacaccatga tcggcaacgc ggtaaatgcc gccgcgcgcc tcatgcagat
     2401 agcgcaggcc ggcgaggcgc tggtttcagg cgagttctat gcttccatcc gccatctcgt
     2461 gccgccgacg cgagtggagt cgcttggcct cgtgaccctg cgcggcaggt ccgagccgat
     2521 gccggtcttc tcgatccggg cctagtggcc tagcacctgg ctcaagaaga gctttgcccg
     2581 gtcggtcttc ggcgcgccga agaactgcgc gggcggggcg gcctcgacga tgcggccgcc
     2641 gtccatgaac accacccggt cggcgaccgt gcgcgcgaac cccatctcgt gcgtgacgac
     2701 aatcatcgtc atgccggaac gcgccagttc ctgcatcacg tccagcacct ctttgatcat
     2761 ctccgggtcg agcgcggaag tcggctcgtc aaacagcatg atcttcggct ccatcgccag
     2821 cgcgcgggca atcgctacgc gctgctgctg cccacccgaa agctgccccg gatatttctc
     2881 cgcctgttcg gggataccga cccgttccag cagtt
//
LOCUS       DASUNT010000481         3898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1030618, whole genome shotgun sequence.
ACCESSION   DASUNT010000481 DASUNT010000000
VERSION     DASUNT010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030618"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VFX94_09690"
     CDS             complement(<1..925)
                     /locus_tag="VFX94_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5768492.1"
                     /translation="MAERILVVDDQRTNAEMVSGLLRNLGYEVDLALDGAEALELVHT
                     RGPDLVISDILMPRLDGFELCRRLRANAATALLPVILVTGAEQGERIKGVEAGADDFL
                     AKPVNWPELFARVKRLLQVKALQDEIKELNTRLEQRVREQVAQLERLSRMKRFFSRAV
                     AEAIVAGGEELLEPHRREITAVFLDLRGFTSFTDRADPDEVLELLRAYHATLGRTVDE
                     FGGTLEHFAGDGVMIFFNDPIEVDNPAERAIRMALALQRAFNPIAEAWLKLGHEVGLG
                     IGIAQGDTTLGVIGFEQRWEYAAIGNVPNLAA"
     gene            complement(938..2044)
                     /locus_tag="VFX94_09695"
     CDS             complement(938..2044)
                     /locus_tag="VFX94_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEX5768493.1"
                     /translation="MPRNAKAALCREHHKPVVVETIAVDPPKRGEATVRLAACGVCHS
                     DLSAVTGTIALPLPLVLGHEGAGVVEEVGEGVSGLAKGDHVIFSFIYMCGKCRFCVAG
                     RPVLCLEQSKALTTPLEGTPRVHDARGAPLGIFSGCGAMAEYATVSAENLIRIDPKIP
                     LDCAALVGCGVTTGVGAVFNTAKVQPGSSVAVFGCGGVGLSVVQGARIAGAERIIAVD
                     TLEPKLEMARNFGATETFLSGKEDVVKFLKKATSGGPDYAFECVGSGELAGAAYRAIR
                     RGGLAVVVGVAKPSDSTSVRTMTLPFEEKTLTGSYFGSCVPRVDFPRMLALYMAGRLK
                     LEELITQRYPIDEAPRAFADMEAGRNARGVIVFQ"
     gene            2071..3498
                     /locus_tag="VFX94_09700"
     CDS             2071..3498
                     /locus_tag="VFX94_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5768494.1"
                     /translation="MLSRDRFFIGGEWVAPSARQTIDVYNAGNGERLGKVPLGDEKDI
                     DAAVAAARGAFERWSGGSAEWRAGFLDKISAGLKARGEELARLIAQEVGMPLKLAARI
                     QVALPIANFANYAKLAREFSFEQRVGNSLVVREPVGVVGAITPWNYPLHQITLKVAPA
                     LAAGCTVVLKPSEIAPLNAFVLAEVAETAGLPKGVLNVVTGTGPKAGEALVRHPGVDM
                     ISFTGSTRAGKRISELASSSVKRVALELGGKSASVILEDADLAAAVKGTLNGCYLNSG
                     QTCTALTRMLVPQSKYEEAAKLAAELAKGFTVGDPLAESTRLGPLTSLTQLERVRGYI
                     KKGVEEGAELLAGGAEPPEGAPAGGYYVRPTVFGKVDNRMTIAQEEIFGPVLSIIAYK
                     DEEDAIRIANDSPYGLAGAVWSKDDAHAQRVARRIRAGQVDVNGGAFNLNAPFGGFKQ
                     SGHGREAGVYGLEEFLEYKSLQLKG"
     gene            3495..>3898
                     /locus_tag="VFX94_09705"
     CDS             3495..>3898
                     /locus_tag="VFX94_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5768495.1"
                     /translation="MRLLRLATGLVVAGFVVGHFLNHSLGVVSIEAMDRLRFVLASWW
                     RSPVGTVLLYGSLLTHFLLALASLYRRSTLRMPLWEAAQLVVGLAIPPLLIGHIVGTR
                     LAWTLLGHSVDYERVVGLLWSSEGSAIRQSLLL"
BASE COUNT          620 a         1346 c         1314 g          618 t
ORIGIN      
        1 gcgcagcgag gttcggaacg ttgccgatcg ccgcatactc ccagcgctgc tcgaagccga
       61 tcacgccgag cgtggtgtcg ccctgcgcga tgccgatgcc gaggcccacc tcgtggccga
      121 gcttgagcca cgcctcggcg atcgggttga acgcgcgctg cagcgcgagc gccatgcgga
      181 tcgcgcgctc ggcgggattg tcgacctcga tcggatcatt gaagaagatc atcacgccgt
      241 cgccggcgaa gtgctcgagc gtgccgccga actcgtcgac cgtgcgcccg agcgtggcgt
      301 ggtaggcgcg cagcagctcg agcacctcgt cgggatcggc acggtcggtg aacgaggtga
      361 agccgcgcag atcgaggaac accgcggtga tctcgcgccg gtgcggctcg agcagctcct
      421 cgccgccggc gacgatcgcc tccgccacgg cgcgcgagaa gaagcgcttc atgcgcgaga
      481 gccgctcgag ctgcgccacc tgctcgcgca cgcgttgctc gagcctggta ttgagctcct
      541 tgatctcgtc ctgcagcgcc tttacctgca acaggcgctt cacgcgcgcg aacagctcgg
      601 gccagttgac cggcttcgcg aggaaatcgt cggcgcccgc ctcgacgccc ttgatgcgct
      661 cgccctgctc cgcgccggtg accaggatca ccggcagcaa cgcggtcgcc gcgttcgcgc
      721 gcagccgccg gcacagctcg aagccgtcga ggcgcggcat caggatgtcc gagatcacga
      781 ggtccggtcc gcgggtatga acgagctcga gcgcctcggc gccgtcgagc gcgaggtcga
      841 cctcgtagcc gaggttgcgc agcagccccg acaccatttc cgcgttggtt cgctggtcgt
      901 ctacgaccag gatgcgctcg gccatccctc tttgacctta ttggaaaacg attacgccgc
      961 gggcattcct gccagcctcc atgtcggcga aggcccgcgg cgcttcgtcg atcgggtagc
     1021 gctgcgtgat gagctcctcg agcttgagcc gccccgccat gtaaagcgcg agcatgcgcg
     1081 ggaagtccac gcgcggcacg cacgagccga aatagctccc ggtcagcgtc ttttcctcga
     1141 acggcagcgt catcgtgcgc actgaggtcg agtcgctcgg cttcgccacc ccgacgacca
     1201 ccgcgagccc gccgcggcgg atggcgcggt aggcggcgcc cgcgagctcg cccgagccga
     1261 cgcactcgaa cgcgtagtcc gggccgccgg aggtggcttt cttcaagaat ttgactacgt
     1321 cttcttttcc actcagaaag gtctctgtcg cgccgaagtt ccgtgccatc tcgagcttcg
     1381 gctccagcgt gtcgacggcg atgatgcgct cggcgcccgc gatgcgcgcg ccctgcacga
     1441 ctgaaagccc gaccccgccg cagccgaaca cggccaccga ggagccgggc tggactttcg
     1501 cggtgttgaa caccgcgccc acgccggtgg tgacgccgca gccgacgagc gccgcgcagt
     1561 cgagcggaat cttcgggtcg atcctgatca ggttctcggc cgagacggtg gcgtattccg
     1621 ccatcgcgcc gcagcccgaa aagatgccga gcggcgcgcc gcgcgcgtcg tgtacgcgcg
     1681 gcgtgccctc gagcggagtg gtgagcgcct tcgactgctc taggcacagc accggccgcc
     1741 cggcgacaca gaagcggcac ttgccgcaca tgtagatgaa ggaaaaaatg acgtggtcgc
     1801 ccttagcgag cccgctcacg ccctcgccca cttcctccac gacccccgcg ccctcgtgtc
     1861 cgagcaccag cggcagcggc agcgcgatcg tgccggtaac cgcggacagg tccgagtggc
     1921 acacgccgca agcggcaagc ctgacggtcg cctcgccgcg cttcggcgga tcgaccgcga
     1981 tggtttcgac cacgacgggc ttgtggtgtt cgcggcacag cgccgctttg gcgttccggg
     2041 gcacgcggtc agtatagcta gaatatcgcc atgctctcga gagaccgctt cttcatcggc
     2101 ggcgagtggg tcgcgccgtc ggccaggcaa accatagatg tctacaacgc gggcaacggc
     2161 gagcgcctcg gcaaagtgcc gctcggcgac gagaaggaca tcgacgccgc ggtcgccgcg
     2221 gcgcgcggcg cattcgagcg atggagcggc ggctcggccg agtggcgcgc cggcttcctc
     2281 gacaagatct ccgccggcct gaaagcgcgc ggcgaagagc tcgcgcggct catcgcgcag
     2341 gaggtcggca tgccgctcaa gctcgcggcg cgcatccagg tcgctctgcc gatcgccaac
     2401 ttcgcaaact atgcgaagct ggcgcgcgag ttttccttcg agcagcgggt cggcaattcg
     2461 ctcgtggtaa gagagccggt cggcgtggtg ggcgccatca cgccctggaa ttacccgctg
     2521 caccagatca cgctcaaggt cgcgccggcg ctcgccgcgg gctgcaccgt ggtgctcaag
     2581 ccctcggaga tcgcgccgct gaatgccttc gtgctcgccg aagtggccga gaccgcgggc
     2641 ctgccgaagg gcgtgctcaa cgtcgtgacc ggcaccgggc ccaaggcggg cgaggcgctc
     2701 gtcaggcacc ccggcgtcga catgatctcg ttcaccggct ccacccgcgc cggcaagcgg
     2761 atctccgaat tggcttcttc ttcagtaaaa cgggtggcgc tggagctcgg cggaaagagc
     2821 gcctcggtca tcctcgagga cgcggacctg gccgcagcgg tgaaaggaac gcttaacggc
     2881 tgctacctca actccggcca gacctgcacg gcgctgaccc ggatgctggt tccgcaatcg
     2941 aaatacgaag aggcggccaa gctggccgca gagctcgcca agggcttcac cgtgggcgac
     3001 ccgctcgcgg agtcgacgcg gctcgggccg ctcacctcgc tcacgcagct cgagcgcgtg
     3061 cgcggctaca tcaagaaggg cgtcgaggaa ggcgccgagc tcctcgcggg cggcgccgag
     3121 ccgccggaag gcgcacccgc gggcggctac tacgtgcgcc cgacggtgtt cggcaaagtc
     3181 gacaaccgca tgaccatcgc gcaggaagag atcttcggcc cggtgctctc gatcatcgcg
     3241 tacaaggacg aggaggatgc gatccgcatc gcgaacgaca gtccctacgg cctcgcgggc
     3301 gcggtgtggt cgaaggacga cgcgcacgcg cagcgcgtcg cgcgccgcat ccgcgccggc
     3361 caggtggacg tcaacggcgg cgcattcaat ctcaatgcgc ccttcggcgg cttcaagcag
     3421 tccggtcacg gccgggaagc cggcgtctac ggcctggagg aattcctcga atataagtcc
     3481 ctgcaattga agggatgaga cttcttcgcc tcgcgacggg gctggtggtc gccggcttcg
     3541 tcgtcgggca tttcctcaac cactcgctcg gcgtggtctc gatcgaggcg atggaccggc
     3601 tgcgcttcgt gctcgcctcg tggtggcgca gcccggtagg cactgtgctt ctgtacggct
     3661 cgctgctcac gcattttctg ctggctctgg cgagcctcta ccgccgaagc acgctgcgca
     3721 tgccgttgtg ggaggcggca cagcttgtcg tcggtctggc gatcccgccg ctgctcatcg
     3781 ggcacatcgt cggcacgcgc ctggcctgga cgctgctcgg ccacagcgtc gactacgagc
     3841 gcgtggtggg acttctctgg tcgagcgagg gatcggcgat ccgccagtcg ctgctcct
//
LOCUS       DASUNT010000482         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_242735, whole genome shotgun sequence.
ACCESSION   DASUNT010000482 DASUNT010000000
VERSION     DASUNT010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242735"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..392)
                     /locus_tag="VFX94_09710"
     CDS             complement(195..392)
                     /locus_tag="VFX94_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768496.1"
                     /translation="MRKQFVTSFWQRAYEKMPEHVRGQYLTQMQSAERWELFVDDLIE
                     FWSRGKNAAVKLFHSPRAKRA"
     gene            complement(385..549)
                     /locus_tag="VFX94_09715"
     CDS             complement(385..549)
                     /locus_tag="VFX94_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768497.1"
                     /translation="MENRYPHYPTTAELYAFEREARRLRAEEMARLIRAATDAVRSVF
                     RTKSKRLQHA"
     gene            629..1573
                     /gene="gcvA"
                     /locus_tag="VFX94_09720"
     CDS             629..1573
                     /gene="gcvA"
                     /locus_tag="VFX94_09720"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HEX5768498.1"
                     /translation="MIYRLPPLNALRVFEAAARHLSFKEAAAELHITQAAVSHQVKSL
                     EEYLGVQLFRRSARGVQLTDAARACLPKLREGFDALAAAVETIRERGEESELAITAPP
                     VFTARWLMPRLAEFSQREPKIELHVFASSKMVDAGALDSSTLVGDLDLRTESAAVQIH
                     LGAGHYPGHRADRLFGVSMAAVANPDLKPSLEVPEHLKHHTLLHDDAMDIVAGGNAWR
                     KWLEAAGVADRVDADRGPHFSSNILSLEAASQKLGVALALRPLVNADIASGRLAAPFD
                     MEVKPGSAYYLVCPEVIADRPAVAAFRKWLLEQAKNSA"
     gene            1605..2015
                     /locus_tag="VFX94_09725"
     CDS             1605..2015
                     /locus_tag="VFX94_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX5768499.1"
                     /translation="MRSRDLSSWMWGEALSMLDRAQRLQRQFFTHANVAWEPPVDIVE
                     TGDTLEMHVALPGVGADAITVAIDSAGITISALRALPCRGEESQIHRLEIPYGRFERF
                     IGLPLADPYSPLELAAKSLQDGILTLTFRRKESP"
     gene            2012..>2656
                     /locus_tag="VFX94_09730"
     CDS             2012..>2656
                     /locus_tag="VFX94_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX5768500.1"
                     /translation="MNDKVAGATAAKAQPMKPLPEDALIIIPMRNTVLFPGVISPVTV
                     GRASSVAAAQEAVRSEKKVGFLLQRNPQTNDVKPEDVYWVGTAGQVVRYITGAESTHH
                     LVVQGQSRFRVLEFLAGWPFLVARVQFIEQKEEMNPQLEARFLQLKAQAVEAISLLPS
                     APEELSVVVQGMTSPALLADMVANLLDIKNEDKQDLLETFDLLRRLDKVLEHLSE"
BASE COUNT          460 a          885 c          864 g          447 t
ORIGIN      
        1 cagttcttgt aggcgcgcag caggagcagg cggatgccgc gatcgctgcg ccggaagacg
       61 accgcgcctg cggcgcgggg gacgggtttc accccgccat tctatcgatc agatgctcgc
      121 cgcccgagcg ggccctagcc agaggaggag gaggaggctt cggatttgct cggacggcga
      181 ggcaggccgg aatgctaagc gcgtttcgcg cgcggggagt ggaacagttt cacagccgcg
      241 ttcttgccgc gcgaccagaa ttcgatcagg tcgtcgacga aaagttccca gcgctcggcg
      301 ctctgcatct gcgtgagata ttgcccacgc acgtgctcgg gcatcttctc gtaggcgcgc
      361 tgccagaagg aggttacgaa ttgcttacgc atgctggagc ctcttcgact tcgtcctgaa
      421 cacggaacgc accgcgtccg tggccgcgcg gatcagccgc gccatctctt cggctcgcag
      481 gcggcgagct tcgcgctcga acgcgtacaa ttccgcggtc gtcgggtagt gcgggtaacg
      541 gttttccatg catgcaatat aggcgccgaa acccggccgt acaaacgatc aaaactcacc
      601 ccttgcataa gaaaaacttg ctcattccgt gatttatcgc ctgccgccgc tgaacgccct
      661 gcgcgtattc gaggccgccg cgcgccacct ttccttcaag gaagcggccg cggagctgca
      721 catcacgcag gcggcggtga gccaccaggt gaagagcctc gaggagtacc tcggggttca
      781 gcttttccgc cgctcggcgc gcggcgtgca gctcaccgat gcggcgcgcg cctgcctgcc
      841 aaagctgcgc gaaggtttcg acgcgctcgc ggccgcggtg gagacgatcc gcgagcgggg
      901 cgaggagtcc gagctcgcga tcaccgcgcc gcccgtattc accgcgcgct ggctcatgcc
      961 gcggctcgcc gagttctcgc agcgcgaacc gaagatcgag ctgcatgtgt tcgcctcgag
     1021 caagatggtg gatgcgggag cgctcgattc gagcacgctg gtcggcgacc tcgacctgcg
     1081 caccgagtcc gcggccgtgc agatccatct gggcgccggc cactatcccg gccaccgcgc
     1141 cgatcgcctc ttcggcgtgt cgatggccgc ggtggcgaat ccggacctga aaccttcact
     1201 ggaggtcccc gagcacctga aacaccacac gctgctgcac gacgatgcga tggacatcgt
     1261 ggcgggcggg aacgcctggc gcaaatggct cgaggcggca ggcgttgccg accgcgtcga
     1321 cgccgaccgg gggccgcact tttcgtccaa catcctctcg ctcgaggcgg cttcgcagaa
     1381 gctcggcgtc gcgctcgcgc ttcggccgct cgtgaacgcc gacatcgcct ccggcaggct
     1441 cgctgcgcca ttcgacatgg aggtgaagcc cgggtcagcc tactacctcg tgtgcccgga
     1501 agtgatcgcc gaccggccgg cggtcgccgc cttccgcaag tggttattgg agcaggctaa
     1561 aaactccgct tgaacccggg ggtattcgcc gcaatctaag cgcggtgaga tcccgcgacc
     1621 tttccagctg gatgtggggc gaagccctgt cgatgctcga ccgggcccag cgcctgcagc
     1681 gccagttctt cacccacgcc aacgtggcgt gggagccgcc cgtggatatc gtcgagaccg
     1741 gagatacgct cgagatgcat gtcgcgctac cgggcgtggg cgcagacgcc atcacggttg
     1801 cgatcgactc cgccggaatc accatctcgg cgctgcgcgc gcttccgtgc cggggcgaag
     1861 agagccagat ccatcgactc gagatcccct atgggcgctt cgagcgcttc atcggcctgc
     1921 cgctcgccga tccctactcg ccgctcgagc tcgcggcgaa gtcattgcaa gacggcatcc
     1981 tcaccctcac cttccgcagg aaggagagtc catgaacgac aaggttgctg gagctacggc
     2041 tgcaaaagcc cagccgatga agccgctgcc cgaggacgcg ctcatcatta ttccgatgcg
     2101 gaacacggtg ctgttcccgg gcgtgatctc gcccgtgacc gtgggacgcg cgagctcggt
     2161 tgccgccgca caggaggccg tgcgcagcga gaagaaggtc ggctttctcc tgcagcgcaa
     2221 tccgcagacc aacgacgtca agccggagga cgtctactgg gtgggcacgg cgggccaggt
     2281 cgtgcgctac atcaccgggg cggaaagcac gcatcacctc gtggtgcagg gccaaagccg
     2341 ctttcgcgtg ctcgagttcc ttgcgggctg gcctttcctc gtggcccggg tgcagttcat
     2401 cgagcagaag gaagaaatga acccgcagct cgaggcccgc ttcctgcagc tcaaggccca
     2461 ggcggtggag gcgatctcgc tcctgccgag cgcgcccgag gagctcagcg tggtcgtgca
     2521 gggcatgacc tcgccggcgc ttctcgccga catggtcgcg aacctgctcg acatcaagaa
     2581 cgaggacaag caggacctcc tcgaaacctt cgacctcctg cgccggctcg acaaggtgct
     2641 cgagcatctt tcggag
//
LOCUS       DASUNT010000483         4323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_767986, whole genome shotgun sequence.
ACCESSION   DASUNT010000483 DASUNT010000000
VERSION     DASUNT010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767986"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1091)
                     /locus_tag="VFX94_09735"
     CDS             complement(117..1091)
                     /locus_tag="VFX94_09735"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX5768501.1"
                     /translation="MSEVLLEVENLVKHFPVGGGMFSPPVAAVRAIDGVSFTLNRGET
                     LGLVGESGCGKTTTGRCILQLERPTSGRIVFEGVDLTTLDEAALRKVRRRVQVIFQDP
                     YSSLNPRMTIGQILAEPLKVHGIVRDAAAREARVQELLQQVGLLPQHAKRYPHQLSGG
                     QRQRVGIARALAMEPSLIICDEPVSALDVSIQAQIINLLEDLQKRLGLTYLFIAHDLS
                     VVRHISDRVAVMYLGKIVEITDRTALYEAPRHPYTRALLSAVPIPDPAVEQTRERRVL
                     RGEVPSPLNPPSGCVFHPRCPIAVGRCSAEVPALREIRRTHWGACHLA"
     gene            complement(1088..2077)
                     /locus_tag="VFX94_09740"
     CDS             complement(1088..2077)
                     /locus_tag="VFX94_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5768502.1"
                     /translation="MAALLEVRNLRTTFTTAAGPVRAVDGVSWDIAEGETVALVGESG
                     CGKSVSALSIMRLVAEPAGRIEAGEILYKGRDLLKLSEPEMRHVRGREIAMVFQEPMT
                     SLNPVLSIGRQLTEGLEIHMNMAPAQAQARAVELLAMVGIPDPQRRLAQYPHHFSGGM
                     RQRMMIAMALACNPSLILADEPTTALDVTIQAQILELMRDLARRLGVAMLIITHNLGV
                     VARYADRVNVMYAGRIIEQAAAREIYANPRHPYTLGLLRSVPRLDEPRRARLDPIHGQ
                     PPDLSRLPPGCAFAPRCAYRVERCSEAPALVEVGRGHTSACWEATRLVKVLAA"
     gene            complement(2082..3272)
                     /locus_tag="VFX94_09745"
     CDS             complement(2082..3272)
                     /locus_tag="VFX94_09745"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5768503.1"
                     /translation="MPGPLAGVKVLELAQIMAGPTCGIMLADLGADVIKVERIPGGDD
                     TRHMDRPSVRGESASFMAMNRNKRGIALNLKLPAAQEALKRMAVRADVLTENYRKGTM
                     EKLGLGYQTLKNVNPALIYCSISGYGRTGPYADKGGYDLIAQGMSGLMSVTGEPGRAP
                     AKSGGPVCDINAGLLGALGVVSAYVHRLKTGEGQLVDTSLFEAGVQQMYWQAAIYFAT
                     GEPPGPTGSAHILSAPYQAFRTADGWVTIGGANQANWERLVRVLNAPQLLQDARFASN
                     AERMKHLQALTESLNAILRAWKSKDLIKALEAAGVPCGPINSIADVAADPHALAREMV
                     VELEHPRAGRTRALGLPIKLSRTPGKVTRPAPTFGQHTREVLAEFGFSAAEIDALYDA
                     KAVA"
     gene            complement(3277..>4323)
                     /locus_tag="VFX94_09750"
     CDS             complement(3277..>4323)
                     /locus_tag="VFX94_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5768504.1"
                     /translation="VFKTTQGEVVIYPASGTGAWEAALVNTLSPGDRVLMVETGHFAT
                     LWQKMATKLGLEVDFLPGNWRYGADPDAIEKRLRADSAKKLKALCVVHNETSTGVASR
                     IAEVRQALDAANHPALFMVDTISSLASIDYRHDEWRVDVTVAGSQKGLMLPPGLSFNC
                     ISPKALAASQSARLTRSYWAWDEMLANGKNGYFPYTPATNLLYGLREALRMLLEEEGL
                     DAVFARHQRHAEATRRAVRAWGLEIFALDPREYSASLTGVLMPAGHDADRVRKAILDA
                     FDLSLGTGLTKLGGKMFRIGHLGDFNDLMLMGTLAGVEMGLGLAGVPVKKEGVQAAME
                     YLAGCAQAPARKVA"
BASE COUNT          683 a         1442 c         1486 g          712 t
ORIGIN      
        1 cgcgacggcc agtgcccgta cgagagtgct cattacgcct cctcctagtt tgagataccg
       61 ccccctgatg ggggtcttgt cctccaagcc tagttaatgg cgatcagggc ctggtgtcaa
      121 gccagatggc aggcgcccca gtgcgtgcgc cggatctccc gcagcgcggg cacttccgca
      181 ctgcagcgac ccaccgcgat ggggcagcgg ggatgaaaaa cgcagcccga gggcggattg
      241 agcgggctgg ggacctcacc gcgcagcacc ctgcgctcgc gcgtctgctc gacggcgggg
      301 tcaggaatcg gcaccgccga gagcagcgcc cgcgtatagg ggtggcgggg cgcctcgtag
      361 agcgcggtgc gatcggtgat ctcgacgatc ttgccgaggt acatgaccgc cacccggtcc
      421 gagatatggc gcaccaccga gaggtcgtgc gcgatgaaga ggtaggtgag ccccaggcgc
      481 ttctgcaggt cctcgagcag gttgatgatc tgcgcctgga tcgacacgtc gagcgccgac
      541 accggctcgt cgcagatgat gagcgagggc tccatggcaa gcgcgcgcgc gatgcccacg
      601 cgctggcgct ggccgccgga gagctggtgc gggtagcgct tcgcatgctg tggcagcaag
      661 ccgacctgtt ggagcagctc ctgtacgcgc gcttcgcgtg cggccgcgtc gcggacgatg
      721 ccgtgcacct tgagcggctc ggcgaggatc tggccgatcg tcatgcgcgg attgagcgag
      781 gagtaggggt cctggaagat cacctggacg cggcggcgca ccttgcgcag cgccgcctcg
      841 tcgagcgtcg tgaggtccac gccctcgaac acgatgcgcc cgctcgtcgg ccgctcgagc
      901 tgcaggatgc aacggccggt ggtggtcttg ccgcagcccg actcgccgac caggccgagc
      961 gtttccccgc ggttgagggt aaagctcact ccgtcgatcg cgcgcaccgc cgccaccggg
     1021 ggcgagaaca tcccgccgcc gaccggaaaa tgcttgacga gattctcgac ttcgaggagc
     1081 acttcgctca cgccgcgagc accttgacga gccgcgtcgc ttcccagcac gcggaggtgt
     1141 ggccccggcc gacttcgaca agcgcaggcg cctcgctgca gcgctccacg cgataggcgc
     1201 agcgtggcgc aaaagcgcag cccggcggca ggcggctcag gtcgggcggt tgtccgtgga
     1261 tcgggtcgag gcgtgcgcgc ctcggctcgt ccagacgggg caccgagcgc aagaggccca
     1321 gcgtataagg atggcgcggg ttggcgtaga tctcgcgcgc cgccgcctgc tcgatgatgc
     1381 ggcccgcgta catcacgttc acacggtcgg cgtagcgcgc caccacgccg agattgtgcg
     1441 tgatgatgag catcgccacg ccgagccggc gcgccaggtc gcgcatgagc tcgaggatct
     1501 gcgcctggat cgtgacatcg agggccgtgg tcggctcgtc ggcaagaatg agcgacggat
     1561 tacaggcgag cgccatcgcg atcatcatgc gttggcgcat gccgccgctg aagtggtgcg
     1621 gatactgcgc gagccggcgc tgcgggtcgg ggatgcccac catggcgaga agctcgaccg
     1681 cgcgcgcttg cgcctgcgcc ggggccatgt tcatgtggat ctcgagcccc tcggtgagct
     1741 ggcggccgat cgacagcacg gggttgagcg acgtcatcgg ctcctggaac accatcgcga
     1801 tctcgcgccc gcgcacgtgc cgcatctcgg gctccgagag cttgagcaga tcgcgcccct
     1861 tgtagaggat ctcgcccgcc tcgatgcggc ccgcgggctc ggccaccagg cgcatgatgc
     1921 tgagcgccga gacggacttg ccgcagcccg actcgcccac gagcgcgacg gtctcgccct
     1981 cggcgatgtc ccacgacact ccatcgaccg cgcgcaccgg cccggcggcc gtggtgaacg
     2041 tggttctcag gttccgcact tcgagaagag cggccatggt cctaggctac cgcctttgcg
     2101 tcatatagag catcgatctc ggcggcggag aagccgaact cggcgagcac ctcgcgcgtg
     2161 tgctggccga aagtcggcgc cggccgcgtg acctttccgg gcgtgcggga aagcttgatc
     2221 ggcaatccca gggcgcgcgt tcgtccggcg cgcggatgct cgagttcgac caccatctcg
     2281 cgcgcgagcg cgtgcgggtc ggccgccacg tcggcgatgg agttgatcgg gccgcacggc
     2341 acgccggcgg cctcgagcgc tttgatcagg tctttgctct tccaggcgcg cagaatggcg
     2401 ttcagggatt cggtcagcgc ctgcaggtgc ttcattcgct ccgcgttgct cgcgaaacgc
     2461 gcatcctgaa gcaattgcgg agcattcagc acccgcacca gccgctccca gttcgcctgg
     2521 ttcgcgccgc cgatcgtgac ccagccgtcg gctgtgcgga acgcctggta gggcgccgaa
     2581 agaatatgcg ccgagccggt cgggcccggc ggttcgccgg tcgcaaagta gatcgccgcc
     2641 tgccagtaca tctgctggac gcccgcctcg aagagcgacg tatcgacaag ctggccttcc
     2701 ccggttttca gtcggtggac gtaggccgaa actacgccga gcgcgcccag gagcccggcg
     2761 ttgatgtcgc acaccgggcc gccggacttg gccggtgcgc ggccgggctc gccggtgacc
     2821 gacatcaagc ccgacatgcc ctgggcgatg aggtcgtacc cgcccttgtc ggcgtaaggg
     2881 ccggtgcgcc cgtagcccga gatcgaacag tagatcagcg ccgggtttac gttcttcaaa
     2941 gtctggtaac ccaggccgag cttttccatc gtgcccttgc ggtagttctc ggtgagcacg
     3001 tcggcgcgca cggccatgcg cttcagcgcc tcctgcgccg ccggcagctt gaggttgagc
     3061 gcgatgccgc gcttgttgcg gttcatcgcc atgaaggacg ccgactcgcc gcgcaccgac
     3121 gggcggtcca tgtgtcgcgt gtcgtcgccg ccggggatgc gctcgacctt gattacgtcc
     3181 gcgccgagat cggcgagcat gatgccgcac gtgggcccgg ccatgatttg ggcgagctcc
     3241 aggaccttca cgccggcgag cgggcccggc atggcgctag gcgactttgc gcgcgggggc
     3301 ctgcgcgcag ccggcgaggt actccatcgc cgcctgcacg ccttccttct tcaccggcac
     3361 gccggcgagc ccaaggccca tttccactcc tgcaagcgtg cccatcagca tgaggtcgtt
     3421 gaaatcgcca aggtgcccga tgcggaacat cttgccgccg agcttcgtaa gcccggtgcc
     3481 cagtgaaaga tcgaaggcat caaggatcgc cttgcgtacg cgatcggcgt cgtgcccggc
     3541 gggcatgagt accccggtga gcgaggccga atactcgcgc gggtcgagcg cgaagatctc
     3601 gagcccccag gcgcgcaccg cacggcgcgt ggcttccgca tggcgctggt ggcgcgcgaa
     3661 gaccgcatcc agtccttctt cctccagcag catcctgagc gcttcgcgca ggccataaag
     3721 aaggttcgtc gccggggtat acgggaagta gccgttcttg ccgttggcga gcatctcgtc
     3781 ccaggcccag tagctgcgag tcaatctggc cgactgggaa gccgcgagtg ctttcggcga
     3841 gatgcaattg aacgacagcc ccggcgggag catgagcccc ttttgcgagc ccgcaacggt
     3901 cacgtcgacg cgccattcgt cgtggcggta gtcgatggag gcgagcgagg agatcgtgtc
     3961 caccatgaag agcgcgggat gattcgccgc gtccagcgct tgcctcactt cggcgatgcg
     4021 cgaggcgacg ccggtggaag tttcgttgtg caccacgcag agggctttca gcttcttcgc
     4081 gctgtccgcg cgcagccgct tctcgatcgc gtctggatcg gcgccgtaac gccagttgcc
     4141 cggcaggaaa tcgacctcga gcccgagctt cgtggccatt ttctgccaca gcgtggcgaa
     4201 atgcccggtc tcgaccatga gcacgcgatc gccgggagaa agcgtgttca cgagcgccgc
     4261 ctcccatgcg ccggttcccg aggcgggata aatcacgacc tcgccctgcg tggtcttgaa
     4321 gac
//
LOCUS       DASUNT010000484         4521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1030727, whole genome shotgun sequence.
ACCESSION   DASUNT010000484 DASUNT010000000
VERSION     DASUNT010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030727"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFX94_09755"
     CDS             complement(<1..225)
                     /locus_tag="VFX94_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX5768505.1"
                     /translation="MEPLATLFAMSFAAATLVPLPSEAALFAYVHIYPERAALAVAIA
                     TAGNTAGGMTSYALGRLIPEKKVDLKAVQWL"
     gene            243..1754
                     /gene="ilvA"
                     /locus_tag="VFX94_09760"
     CDS             243..1754
                     /gene="ilvA"
                     /locus_tag="VFX94_09760"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEX5768506.1"
                     /translation="MTKGYLERILKARVYDVASESPLEPAPALSRRLGNHLLLKREDL
                     QPIFSFKLRGAYNKMAGLPRRRLARGVIAASAGNHAQGVALAAQRLRCRAVIVMPVTT
                     PRIKVNAVAALGAEVVLHGDSYAEAYKKSLYLKSKRGLSFVHPYDDPDVIAGQGTIGM
                     EILRQHPRPIDAIFVPVGGGGLIAGIAAYVKTINPKIRIVGVEPVDAAAMQQSLRMKR
                     RIRLDHVGLFADGVAVKEVGRETFRLCKALVDEMVLANTDAMCAAIKDVFEDTRVVLE
                     PAGALAIAGAKAWVERRGVRGQTLVAIASGANTNFDRLRFIAEEAELGEHREAILAVT
                     IPERPGSFRKFCALLGARNVTEFNYRIADSKDAHIFVGIEVQGRNETARIVRNLRRHG
                     LTTLDLSDNEMAKLHARHMVGGRAPFAKNELLYRFEFPERPGALMRFLEAMRGGWNIS
                     LFHYRNQGADYGRVLVGIQVPRNELGQFRTFLKKLGYPYADETRNPAYKLFLL"
     gene            1757..2404
                     /locus_tag="VFX94_09765"
     CDS             1757..2404
                     /locus_tag="VFX94_09765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5768507.1"
                     /translation="MAGRAPFLPKKISRYIHEHAVREPEILRELRAATASLPNAGMQI
                     GADQGQFMALLVRAIGARRCLEIGTFTGYSALAVALALPEDGRIVCCDLSEEWTAIGK
                     PFWQKAGVDRKIDLRIGPALGILKDLSGPFDFVFIDADKTNYENYYEACLPMVRRGGI
                     IALDNTLWSGWVADRGHDDADTAALRTFNDKLHRDERVEMSLLPLGDGVTLALKK"
     gene            complement(2439..3134)
                     /locus_tag="VFX94_09770"
     CDS             complement(2439..3134)
                     /locus_tag="VFX94_09770"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX5768508.1"
                     /translation="MKLHVPYMDPFKLKTWRRAERERLLAERAALDPATLDAWRRRID
                     GYLERSFPGLARRRLAFCWPIKGEYDARHLARTLRARGALTALPVVVAPKSPLVFREW
                     HPGIVLARGPLDIPYPADSQQLTPEAVLLPMNGWDPQGYRLGYGGGFFDRTLAALPKK
                     PLTIGISYEMAKLDTIHPQAWDIPMDYVVTERGVYRRDPEGLAFLGEPDPGSGTLASP
                     VCFANEDYFGGKK"
     gene            3145..>4521
                     /locus_tag="VFX94_09775"
     CDS             3145..>4521
                     /locus_tag="VFX94_09775"
                     /inference="COORDINATES: protein motif:HMM:NF026068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768509.1"
                     /translation="MFRKLAAGVLCGLLAHTAPAKDAKHSADEAFLTAYDGYRAGDPI
                     KFARAAKNLESHLLAPWLDYWRLSMRLEDADNREVREFFATHPNQFVAERMRADWLRV
                     LGKRGDWPEFDRQAARYSREDLEVGCYRSLSRLEQGDDSVLNEASAMWLEPAELPEGC
                     QRLSNVLWSRGRLSVTDVWRRVRVLFEFGQTSAAKTALAFLPKNEAPDERMLAEAARQ
                     PRRFIERLPRYLESRAAHEVAVLGAMRYARNDAAAAAAALEGALAERLSDADLGFLWG
                     IVGYEAAREHHNDALKWFARAGDTALDDRHLVWKVRAALRDGEWQVVRDSIERMSFGV
                     AHQPAWVYWYGRALAALGDEMGSRAYFLRIAGQTDFYGLLAAEELGYVLALPEIAHVP
                     TEADVAAAAADPGLKRALELIRLGLRVEGVREWGFTVRSFDDERLLAAAELARRNGVY
                     DRAIATADR"
BASE COUNT          764 a         1477 c         1519 g          761 t
ORIGIN      
        1 aagccactgc acggctttca ggtcgacctt tttctccgga atcaaccttc ccagggcgta
       61 cgacgtcatg ccgccggcgg tgttgcctgc ggtggcgatt gctaccgcga gcgccgcgcg
      121 ctcggggtag atatgaacgt aggcgaacag cgccgcctcg gaaggcagcg gaacgagcgt
      181 cgcggcggcg aagctcatgg cgaacaaagt cgcgagtggc tccatgctaa attccggcgg
      241 ctttgaccaa gggctacctc gagcgaatcc tgaaggcgcg ggtctacgac gtggcgagcg
      301 agtcgccgct cgagccggcg ccggcccttt ccaggcgcct cggcaatcac ctgctcctca
      361 agcgcgaaga cctgcagccg atattctcgt tcaagctgcg cggcgcctac aacaagatgg
      421 ccggattgcc gcgccgccgc ctcgcgcgcg gcgtgatcgc ggcgagcgcg ggaaaccatg
      481 cgcaaggcgt cgcgctcgcc gcccagcgcc tgcgctgccg cgcggtgatc gtgatgcccg
      541 tcacgacgcc gcgcatcaag gtgaacgcgg tcgctgcgct cggcgccgag gtcgttttgc
      601 atggcgactc ctacgcagag gcttataaga aatcgcttta tctcaaaagc aagcgcggct
      661 tgagcttcgt ccatccttac gacgaccccg acgtcatcgc gggccagggt acgatcggca
      721 tggagatcct gcgccagcat ccgcggccga tcgacgcgat cttcgtcccg gtcggcggcg
      781 gcggcctcat cgcgggcatc gccgcgtacg tgaagaccat caatccgaaa atccgcatcg
      841 tcggcgtcga gcctgtcgac gcagcggcca tgcaacaatc cttgcgaatg aagaggcgca
      901 tccgcctcga tcacgtcggc ctcttcgccg acggggttgc ggtgaaggag gtcggccgcg
      961 aaaccttccg gctctgcaag gcgctggtgg acgaaatggt cctcgccaac accgacgcga
     1021 tgtgcgcggc catcaaggac gtgttcgagg acacgcgcgt ggtgctcgag ccggcaggtg
     1081 cgctcgcgat cgccggcgcc aaggcgtggg tcgagcgacg cggcgtgcgc ggacagacgc
     1141 tcgtcgcgat cgcctcgggc gcgaacacca acttcgatcg cctgcgcttc atcgccgagg
     1201 aagccgagct cggggagcac cgggaggcga ttcttgccgt gacgattccc gagcgcccgg
     1261 gcagcttccg caagttctgc gcgctgctcg gcgcgcgcaa cgtgaccgag ttcaattacc
     1321 gcatcgccga ctcgaaggac gcgcatatct tcgtcggcat cgaggtgcag gggcgcaacg
     1381 agacggcgcg catcgtgcgc aacctgcgcc gccacgggct gacgacgctc gacctttccg
     1441 acaacgagat ggcgaagctg catgcccgcc acatggtcgg cgggcgcgcg ccgtttgcca
     1501 agaacgagct tctgtatcgc ttcgaatttc ccgagcggcc gggtgcgctg atgcgcttcc
     1561 tggaggcgat gcgcggcggc tggaacatca gccttttcca ctaccgcaac cagggcgcgg
     1621 actacggccg cgtgctggtg ggaatacagg tgcctcgcaa cgagctcggt cagttccgca
     1681 cctttctcaa gaagctcggt tacccgtacg cggacgagac gcgcaacccc gcgtacaagc
     1741 tgtttctcct ctagccatgg ccggccgcgc acccttcctg ccgaagaaga tcagccgcta
     1801 tatccacgag cacgcggtgc gcgagcccga gatcctgcgc gagctgcgcg ctgcgacagc
     1861 gtcgctcccg aacgccggca tgcaaatcgg cgcggaccag ggacagttca tggcgctgct
     1921 cgtgcgggcg atcggcgcgc gccgctgcct ggaaatcggc accttcactg gttacagcgc
     1981 gctcgccgta gcgctcgcac tcccggaaga cggccgcatc gtgtgctgcg acctgagcga
     2041 ggaatggact gcgatcggca agccgttctg gcaaaaggcg ggcgtcgata gaaagatcga
     2101 cctaaggatc ggtcctgcac ttggaatcct gaaagacctg agcggtcctt tcgactttgt
     2161 cttcatcgac gccgacaaga ccaactacga gaactactac gaggcttgcc tgccgatggt
     2221 ccgccgcggc ggcatcatcg cgctcgacaa cacgctctgg tcgggctggg tggccgaccg
     2281 cgggcatgac gatgccgata ccgcggcgct gcggacgttc aacgacaagc tgcatcgcga
     2341 cgagcgagtc gagatgtcgc ttctgccgct cggcgacggc gtgacgctcg ccctcaagaa
     2401 gtgattcggg tgacgggtcg aattggctcc gtcccggatc acttctttcc gccgaagtag
     2461 tcctcgttgg cgaagcatac cggcgaggcg agcgtgcccg accccgggtc cggttctcca
     2521 agaaaagcga gcccctcggg atcgcgccgg tacaccccac gttcggtcac cacatagtcc
     2581 atggggatgt cccaggcctg cgggtggatg gtgtcgagct tcgccatttc gtaggagatt
     2641 ccgatcgtga gcggtttctt cggcaaggct gcgagcgtgc ggtcgaaaaa tccgccgccg
     2701 tagccgagcc ggtaaccctg cggatcccac ccgttcatgg gcagaagcac cgcttccggg
     2761 gtcaattgtt gtgaatcggc cggatatggg atgtcgagcg ggcccctcgc gagcacgatg
     2821 ccggggtgcc attcgcggaa cacaagtgga ctttttggcg ccacgacgac cggcagcgcg
     2881 gtcaacgcac cccgggcccg cagcgttctg gcaagatgac gggcgtcgta ttcgcccttg
     2941 attggccagc agaaagccag ccggcggcga gcgagaccgg ggaaagagcg ctcgaggtag
     3001 ccgtcgatgc ggcggcgcca ggcatcgagc gttgcggggt caagggcggc acgctcggcg
     3061 agcagccgct cgcgctccgc ccggcgccaa gttttaagct taaacggatc catgtagggg
     3121 acgtgtagtt tcatgattcg taaggtgttc cgcaagctcg cggccggcgt tttgtgcggc
     3181 ctcctggcgc atacggcgcc cgccaaggac gcgaagcatt cggccgatga ggcgttcctt
     3241 accgcctatg acggctaccg cgccggcgat cccatcaagt ttgctcgtgc ggcgaagaac
     3301 ctcgaaagcc acctgctcgc gccatggctg gattactggc ggctttccat gcggctcgag
     3361 gacgcagaca accgcgaagt gcgcgagttc ttcgccaccc atcccaacca gttcgtcgcg
     3421 gaacgcatgc gcgccgactg gctgcgtgtg ctgggcaagc ggggcgactg gcccgagttc
     3481 gaccgccagg ccgcgcgcta tagccgcgaa gacctggaag taggttgcta ccgctcgctc
     3541 tcgcgcctgg agcaggggga cgattcggtg ctcaacgagg cgagcgcgat gtggctcgag
     3601 cccgccgagc tgccagaggg ctgccagcgc ctgagcaacg ttctgtggtc ccgcggccgc
     3661 ctttcggtca ccgacgtgtg gcggcgcgtt cgagtcctgt tcgagtttgg acagacaagc
     3721 gcagccaaga ccgcgcttgc tttcctgccg aagaacgagg cgcccgatga gcgaatgctt
     3781 gcggaggccg cccgccagcc caggcgcttc atcgagcgcc tgccgaggta cctggaatcg
     3841 cgcgcggcgc acgaggtggc ggtgctcggc gcgatgcgct acgcgcgcaa cgacgcggcg
     3901 gctgccgccg cggcgctcga gggagcgctt gcggagcggc tttccgatgc cgacctcggg
     3961 tttctgtggg gaatcgtcgg ctacgaagcc gcgcgcgagc atcataacga cgcgctgaag
     4021 tggtttgcac gcgccgggga cactgcgctc gatgaccgcc acctcgtctg gaaagtgcgc
     4081 gccgcgctgc gcgatggcga atggcaggtg gtgcgcgatt cgatcgagcg catgtccttc
     4141 ggcgtcgcgc accagccggc ctgggtgtac tggtacgggc gcgctctcgc cgcccttggc
     4201 gatgagatgg gcagccgcgc ctacttcctg cgcatagctg ggcagaccga cttctacggc
     4261 ctgctcgccg cggaagagct cggctatgtc cttgcgctgc ccgaaatcgc ccatgtgccg
     4321 accgaggccg atgtcgctgc ggccgccgcc gatccgggtc tcaagcgcgc gctcgagctg
     4381 atccgtcttg gcctgcgcgt ggaaggggtg cgtgaatggg gcttcaccgt gcgttcgttc
     4441 gacgacgagc gcttgctggc ggccgccgag cttgcgcgcc gcaacggggt ctacgaccgg
     4501 gcgattgcca ccgcggaccg t
//
LOCUS       DASUNT010000485         2765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_418460, whole genome shotgun sequence.
ACCESSION   DASUNT010000485 DASUNT010000000
VERSION     DASUNT010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418460"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1555)
                     /locus_tag="VFX94_09780"
     CDS             complement(<1..1555)
                     /locus_tag="VFX94_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5768510.1"
                     /translation="MVKSLDRKLLRDLWSLKTQVVSIALVIACGIGGFIGSFSTYGSL
                     LWSRDNYYATARFPHIFATAKRAPLSLLERIRNIPGAVEVEARVVRDAQLSIPGVVPP
                     MIARLIGHDFEHPGGMNRLTLKSGRWPAPNARGEVVVNQRFLEARSLTLGDRVNVLLN
                     GKLERVTLVGTALSPEYIFATRGGGMPDDEWFAVLWIDARTLAAAFNLEGAFNSVLLR
                     IENSASPEAMLDTLDKLLEPYGGFGAVTRDEQVSHKIITQEISQQRIFGTVLPAIFLA
                     VAAFILNVVLHRQVHAQRGEIAALKALGYEDRWIAWHYLKFASVIVLVGTLIGIGLGW
                     WLGQGMTGLYTDLFHFPQFNFFMPGWLALAGGGAALAAAFGGAFAATRGVLKLHAAEA
                     LRPPAPAEFRPLLLERLGYVELLTSAQRMIMRNLERRPVRAATTVAGIAGSVAILIAG
                     TFWGDALEWFIDVQFNQVTRADVNVGFAEPVSRNVRRELERLPGVAQVEVSRAIPVRL
                     RAGYRSYRTG"
     gene            complement(1549..2253)
                     /locus_tag="VFX94_09785"
     CDS             complement(1549..2253)
                     /locus_tag="VFX94_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5768511.1"
                     /translation="MRVFAARGLAKTYRMGETEIHALRGVDLEISAGEFIVLLGPSGS
                     GKSTLLNILGGLDTPTSGQIVFKEEEELSKADEAALTRYRRQHVGFVFQFYNLIPSLT
                     ARENVALVTDIAAKPMPAAEALAWVGLGERLDHFPSQLSGGEQQRVAIARAIVKRPDV
                     LLCDEPTGALDYETGKLVLEVIARINAELGTTAMVITHNAAIAAIADRVLRLGDGRIV
                     GVEKPARKLTPAEISW"
     gene            2308..>2765
                     /locus_tag="VFX94_09790"
     CDS             2308..>2765
                     /locus_tag="VFX94_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5768512.1"
                     /translation="MPHREIVASHLVERAARARGHETAGGVLARLAKEKPASVELVLV
                     VDGHDRLQGVVPLAKLFAAAAQAKLKDIVAHGFPIVRPDTDQEHAASLALHHGVDALP
                     VVDAKARALGVMPSQALLQVLRREHVEDLHRLAGIEREAAQARHAIEDPPL"
BASE COUNT          458 a          934 c          960 g          413 t
ORIGIN      
        1 tgccggtgcg gtagctgcgg tagccggcgc gcaggcgcac cggtatcgcg cggctcacct
       61 cgacctgcgc cacgcccggc agccgctcga gctcgcggcg cacattgcgc gataccggct
      121 cggcgaagcc gacgttcaca tcggcgcgcg tcacctggtt gaactgcacg tcgatgaacc
      181 actcgagcgc gtccccccag aaggtgccgg cgatgaggat cgcgaccgag ccggcgatcc
      241 ccgccaccgt ggtggcggcg cgcaccggcc ggcgctcgag attgcgcatg atcatgcgct
      301 gcgcggaggt gaggagctcg acatagccga ggcgctcgag gaggagcggc cgaaactcgg
      361 ccggcgccgg cgggcgcagc gcctcggcgg cgtgcagctt gagcacgccg cgcgtggcgg
      421 cgaaagcgcc gccgaacgcg gcggcgagcg cggcgccgcc gccggcgagc gccagccacc
      481 ccggcatgaa gaagttgaat tgcgggaagt gaaagagatc agtgtaaaga cccgtcattc
      541 cctgccccag ccaccagccc agcccgattc cgatcagcgt gccgacgagc acgatgaccg
      601 aagcgaactt gaggtagtgc caggcgatcc agcggtcctc gtagccgagc gccttgagcg
      661 ccgcgatctc gccgcgctgc gcgtgcacct gccggtggag caccacgttc aggatgaagg
      721 cggcgaccgc gaggaagatc gccggcagca ccgtgccgaa gatgcgctgc tggctgatct
      781 cctgcgtgat gatcttgtgc gacacctgct cgtcgcgtgt caccgcgccg aagccgccgt
      841 agggctcgag cagcttgtcg agcgtatcga gcattgcctc gggcgaggcg ctgttctcga
      901 tcctgagcag caccgagttg aacgcgcctt cgaggttgaa tgcggcggcg agcgtgcgcg
      961 cgtcgatcca gagcaccgcg aaccactcgt cgtccggcat gccgccgccg cgcgtagcga
     1021 agatgtactc ggggctgagc gccgtgccga ccagcgtgac gcgctcgagc ttgccgttga
     1081 ggagcacgtt cacgcggtcg ccgagcgtga ggctgcgcgc ctcgaggaag cgctggttca
     1141 ccaccacctc gccgcgcgca ttcggcgccg gccagcggcc gctcttgagc gtaagccggt
     1201 tcatgccgcc gggatgctcg aagtcatgac cgatcaggcg cgcgatcatg ggcggcacca
     1261 ccccgggaat ggaaagctgc gcatcgcgca ccacgcgcgc ctcgacctcg acggcgcccg
     1321 gaatattgcg gattcgctcg agaagcgaca acggcgcgcg cttcgccgtg gcgaagatgt
     1381 gcgggaagcg cgcggtggcg taatagttgt cgcgtgacca cagcagcgac ccgtacgtcg
     1441 agaacgaccc gatgaagccg ccgatgccgc aggcgatcac gagcgcaatc gagaccacct
     1501 gcgtcttgag cgaccacagg tcgcgcagca gcttgcggtc gaggctcttc accatgagat
     1561 ctccgcgggc gtcaacttgc gcgcgggctt ctccacgccg acgatcctgc cgtcgccgag
     1621 gcgcagcacc cggtccgcaa tcgcggcgat cgcggcgttg tgggtgatca ccatggccgt
     1681 cgtgccgagc tctgcgttga tgcgggcgat cacctcgagc acgagcttgc cggtttcgta
     1741 gtcgagcgcg ccggtcggct cgtcgcagag gagcacgtcc gggcgcttga cgatcgcgcg
     1801 cgcgatcgcg acgcgctgct gctcgccgcc cgagagctgc gacggaaagt gatccagccg
     1861 ctcgccgagg cccacccaag cgagcgcctc cgcggccggc atgggcttcg cggcgatatc
     1921 ggtgacgagc gcaacgttct cgcgcgcggt gaggctcggg atcaggttgt agaactggaa
     1981 cacgaagccc acgtgctgcc ggcggtagcg cgtcagcgcc gcctcgtctg ccttggagag
     2041 ctcctcctcc tccttgaaaa cgatctgccc cgaagtcggg gtgtccagac cgccgagaat
     2101 gttgaggagc gtggatttgc cgctgcccga aggaccgagg agcacgatga actcgccagc
     2161 cgagatctcg agatctaccc cgcgcagcgc gtgaatctcg gtctcgccca tgcggtaggt
     2221 cttggcaagg ccgcgggcgg caaagacgcg catttgacca ttgtcgctca gcgtgccggc
     2281 aggctcttat tagggtgaaa taacgggatg cctcaccgcg aaatcgtcgc gtcgcacctg
     2341 gtggagcgcg ctgcgcgcgc gcgcggccac gagacggcgg gcggcgtgct cgcgcgcctc
     2401 gcaaaagaaa aaccggcgag cgtcgagctt gtcctcgtcg tggacggcca cgaccgtttg
     2461 cagggcgtcg tgccgttggc gaagcttttc gccgccgccg cgcaagcgaa actgaaggac
     2521 attgttgcgc atggctttcc catcgttcgc ccggacacgg accaggagca cgccgcttcg
     2581 cttgcgctcc accacggcgt agacgcactg cccgtggtcg atgcaaaggc ccgggcgctc
     2641 ggcgtgatgc cttcgcaggc gctccttcag gtgctgcgcc gcgagcacgt ggaggacctt
     2701 caccggcttg ccggcatcga gcgcgaggcc gcgcaggcgc ggcacgcgat cgaggacccg
     2761 ccgct
//
LOCUS       DASUNT010000486         3574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1118952, whole genome shotgun sequence.
ACCESSION   DASUNT010000486 DASUNT010000000
VERSION     DASUNT010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118952"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFX94_09795"
     CDS             <1..180
                     /locus_tag="VFX94_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768513.1"
                     /translation="DLLCRFMHWLAPLSQITREEQQVLGVRAGGKVLADGEEGADEIE
                     DASLRDPGPGSQIAA"
     gene            complement(177..557)
                     /locus_tag="VFX94_09800"
     CDS             complement(177..557)
                     /locus_tag="VFX94_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768514.1"
                     /translation="MTFTRVVLYTGADGRARWREEPIELPEGTPQAMLSRVFPSRGYQ
                     LRTSPVGFRSDWHCTAQPQWVFILSGEMEIGLQDGSSRVFRPGEHFFSADTLPEGASF
                     DPKVHGHWSAQRGPGPLVTLFVKT"
     gene            610..1821
                     /locus_tag="VFX94_09805"
     CDS             610..1821
                     /locus_tag="VFX94_09805"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEX5768515.1"
                     /translation="MKWIATGLLVLAAVVYVISEKLDYYYLAAFSEAAMVGALADWFA
                     VVALFRRPLNLPIPHTAIIPRNKERIARGLSQFIQQNFLSSAAVVQRIAEFRPADTLC
                     RWLLKAENVETVAHYATRFAAYALSAVDDERVRRFLQRNLAGLLRSADFASAAAQILD
                     ILTENRRHHALLDAALNALDDVLSREETRRYIAVEVGKSAPLLKRISDWFQLQLDERA
                     ALKIAELAIAKVHEVREDRHHELRRRFDEFVARFIEKLKTDPSLRGKVETLQKEILES
                     PALADYIGGLWQEFRAWLGEDLKRASSVTHERIAGMVRAFGQRLEADREIRQWIDEQI
                     LTAIPPLVDEHRAKIGRFIEDQINGWQEEKLVDELEHHIGADLQYIRINGTLVGGLAG
                     LLIAAFTDLAR"
     gene            complement(1818..2126)
                     /locus_tag="VFX94_09810"
     CDS             complement(1818..2126)
                     /locus_tag="VFX94_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768516.1"
                     /translation="MRIASSIVFSLLCSAVLAAPPPLEIDPNTRIQGGADQRGSGAAA
                     GAGMRRDNKMDSEPRLEAPGNNAQRPDRVDPDRDKPFSERKPGERDQAEERKKSQPNP
                     "
     gene            complement(2360..3436)
                     /locus_tag="VFX94_09815"
     CDS             complement(2360..3436)
                     /locus_tag="VFX94_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX5768517.1"
                     /translation="MKVTAVDTCVLTVPTPKPMALQYPQHKLVVAQIATDEGLHGLGY
                     SLVFGGGGAEAVHAYVDRLKPALIGEDPTYVGYLWEKMFRVDMGMKKQGAAAYALSAL
                     DIGLWDLTGKAFGKPLFKLWGAVRHRIPCYGSGGWAKYTVEDLVREAEKYAGYGCKYY
                     KMKIHDPDPKVNRARVEAVRRALGAGVRLMVDVNQRLDVLGNQRQAALLEEFDLVWYE
                     EPVLADDFESLEQVAASIRIPVATGENNYTRYEFRDIIQRKAARYLMPDVCRANGFSE
                     TLKIGHLAAAHGVAVSPHVVHEISLHVVGALANGFLVEFMDWAPPDLFEEVPACEGGR
                     FRIPDRPGHGMSLARGAIEKYRSR"
BASE COUNT          563 a         1205 c         1196 g          610 t
ORIGIN      
        1 gatcttcttt gcaggttcat gcactggctg gcgcccctca gccagatcac gcgcgaggag
       61 cagcaggtcc ttggcgtgcg cgccggcggc aaggtgctgg ccgatggcga ggaaggcgcc
      121 gacgagatcg aagacgcttc gctgcgcgac ccggggcccg gaagccagat cgccgcctag
      181 gtcttaacga acaacgtcac gagcgggccg gggccgcgct gcgcgctcca gtgcccgtgc
      241 accttcggat cgaagctcgc gccctcgggc agcgtgtcgg cggaaaagaa atgctcgccg
      301 ggacggaaca cgcgcgagct gccgtcctgc agtccgatct ccatctcgcc cgagagtatg
      361 aaaacccatt gcggctgcgc cgtgcaatgc cagtcgctgc ggaatccgac cgggctcgtc
      421 cgcagctgat agccgcgcga aggaaacacg cgcgacagca tcgcctgcgg cgtgccctcg
      481 ggcagctcga tcggctcctc gcgccagcgc gcgcgcccgt cggcgccggt gtacagcacc
      541 accctggtaa acgtcatggc gcgtcccccc gcattaggat agcagcgccg aggaggacga
      601 cgcatgcgca tgaaatggat cgcgaccggg ctgctcgtgc tggccgccgt ggtgtacgtg
      661 atatccgaga agctcgacta ttactacctt gcggcgttca gcgaggccgc gatggtgggc
      721 gcgctcgccg actggttcgc cgtagtcgcg ctttttcgcc ggccgctcaa tctgccgatc
      781 ccgcataccg cgatcattcc gcgcaacaag gagcgcatcg cgcgcggtct gtcgcagttc
      841 atccagcaga acttcctgtc ctccgccgca gtagtgcagc gcatcgccga gttcaggccc
      901 gccgacacgc tgtgccgctg gctgctgaag gccgagaacg tcgagacggt cgcgcactac
      961 gcgacgcgct tcgcggctta cgcgctcagc gcggtcgacg atgagcgcgt gcggcgcttt
     1021 ctccagcgca acctcgccgg cctgctgcgc agcgcggact tcgcctcggc ggccgcacag
     1081 atcctcgaca tcctgaccga gaaccggcgc caccacgcgc tgctcgacgc cgcgctcaac
     1141 gcgctcgacg acgtgctctc ccgggaggag acgcggcgct acatcgccgt cgaggtcggc
     1201 aagagcgcgc cgctgctcaa gaggatcagc gactggttcc agctccagct cgacgagcgc
     1261 gccgcgctga agatcgccga gctcgcgatc gccaaggtcc acgaggtgcg cgaagaccgc
     1321 caccacgagc tgcggcggcg cttcgacgag ttcgtcgcgc ggttcatcga gaagctgaag
     1381 accgatccat cgctgcgcgg gaaagtcgaa accctgcaga aagagatcct ggaaagcccg
     1441 gcgctcgccg attacatcgg cgggctgtgg caggagttcc gcgcctggct cggcgaggac
     1501 ctgaagcgag cgtcctcggt gacccacgag cgcatcgccg gcatggtgcg cgccttcggc
     1561 cagcgcctgg aggccgaccg cgagatccgc cagtggatcg acgagcagat tctcaccgcg
     1621 ataccgccgc tcgtcgacga gcaccgggcg aagatcgggc gcttcattga ggatcaaatc
     1681 aacggctggc aggaggagaa gctggtcgac gagctcgagc accacatcgg cgccgacctg
     1741 cagtacatcc gcatcaacgg cacgctcgtc ggcgggctcg ccgggctgct catcgccgcg
     1801 ttcaccgacc tcgcgcgcta agggttgggc tggcttttct ttctttcttc ggcttggtcc
     1861 cgctcaccgg gcttgcgctc gctgaacggc ttgtcccggt ccggatcgac tctgtcgggg
     1921 cgctgagcgt tgttgccggg ggcttcgagc cgcggctcgg aatccatctt gttgtctcgg
     1981 cgcattcccg ctccggcggc ggcgccgctt ccgcgctgat cggcgccgcc ctggatcctg
     2041 gtgttcgggt cgatttcgag cggcggcggc gccgcaagga ccgccgagca cagcaggctg
     2101 aagacaatcg atgaagcgat tcgcatggcc cccaaattgc tgcaatcgcc atgccatgaa
     2161 gctgcttcta tccattctgc tcgtgttggg tattacggca tgcgaccgcg atgtaccggg
     2221 gagcggcagc gcgaacgagg gcaaggcggc ccagtccgct ggcggcgtga gctccgatga
     2281 ggggaaggcg gcgcgcagcg ccggccgtta agaattcggg acggagcccg aatccattag
     2341 cgctccgtcc ccgaattccc tagcggctgc ggtatttctc gatcgcgccg cgcgcgagcg
     2401 acatgccgtg cccgggccga tcggggatgc ggaagcgccc gccctcgcac gcgggcacct
     2461 cctcgaagag gtccggcggg gcccagtcca tgaactcgac gaggaagccg ttggcgagcg
     2521 cgccaaccac gtgcagcgag atctcgtgca cgacgtgcgg cgagaccgcg acgccgtgcg
     2581 cggcggcgag gtggccgatc ttcagcgtct cgctgaaacc gttggcgcgg cacacgtcgg
     2641 gcatgagata gcgcgccgcc tttctctgga tgatgtcccg gaattcgtag cgggtgtagt
     2701 tgttttcgcc ggtggccact ggaatgcgga tgctcgcggc aacttgctcc agcgattcga
     2761 aatcgtcggc gagcaccggc tcctcgtacc acacgaggtc gaactcctcg agcagcgccg
     2821 cctggcgctg gttgccgagc acgtcgagcc gctggttgac gtcgaccatc aggcgcacgc
     2881 cggcgccgag cgcgcggcgc accgcttcga cgcgcgcgcg gtttactttc ggatccgggt
     2941 cgtggatctt catcttgtag tacttacagc cgtagccggc gtacttctcg gcttcgcgca
     3001 ccaggtcctc caccgtgtac ttcgcccagc cgccgctgcc gtagcagggg atgcgatggc
     3061 gcaccgcgcc ccagagcttg aagaggggct tgccgaacgc cttgccggtg aggtcccaca
     3121 ggccgatgtc gagcgcggag agggcgtagg cggccgcgcc ctgcttcttc atccccatgt
     3181 cgacccggaa catcttctcc cagaggtagc cgacgtaggt cgggtcctcg ccgatcagcg
     3241 cgggcttcag gcgatcgacg taggcgtgca ccgcctcggc cccgccgccg ccgaacacca
     3301 ggctgtagcc caggccgtgg aggccttcgt cggtggcgat ctgggcgacg acgagcttgt
     3361 gctgcgggta ctgcaacgcc atcggcttgg gcgttgggac ggtgaggacg caggtgtcga
     3421 cggcggtgac cttcatgccg tcgcgatgct agctcacttc ttcggctttt ccttcttttc
     3481 gtctgtcttc ggcgctttct ccggcgcgct gctgccgccc gcggcggtgg gcttgatctt
     3541 gatccgcgta ttcgcgtcga tccagatgaa gccg
//
LOCUS       DASUNT010000487         2712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_943049, whole genome shotgun sequence.
ACCESSION   DASUNT010000487 DASUNT010000000
VERSION     DASUNT010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943049"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2712
                     /locus_tag="VFX94_09820"
     CDS             <1..>2712
                     /locus_tag="VFX94_09820"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX5768518.1"
                     /translation="AVSIIASKLKRRLAWTLGGIVVAAAALIALAPLLIDARAVQAEL
                     QRRLSDALGGQVSWQELDVRLLPAPRGELRALRIDVPGKLEASADAVDVHLRLWPLLR
                     GQPEISSITVERPRIRVAPRGDAPEEQKPIDVMALYRAVMEPAANALQDFAPRMELQL
                     NEAVVAVGEQFMLRDLRARARTDSKGVELEGSSAATLWRRLTVQARFEYADLSARASA
                     VVEGLSFDKDIPAAALRADVRTDGRSALECDFDGSVGTLVPAVKGKLLAAAGKPLELA
                     ADLGGMDMPQALALARAKHLPLDLMESAEGKISLAATAVVQEPWRADVSLVKSDAAIK
                     LAALPWKISPRAARVAVTRERVTVSGLEGFIEKSAFSAVQADIDLETNRLASASGRAR
                     LELAQWLPWLKKTLPLEEIASLSGEADVALDRLALAFDRPAAADFDVLVTPRKVRAAV
                     KALPVPVSVTSGSVRAASRRILLQNLAGSLGNSPFSSLTAQIDLGKTPRVSSASARAS
                     LRLDQWFPWLREKLPLQDVASVSGQADVTLNRLALRFDRPAEAEFDAVVTPRKVSAAV
                     KALPVPVRVTGGSVRAGRSRVLLRDLEGSLGASAFGNLNAQIELGKTPLVSSASGRAS
                     VQLEQWFPWLKGALLFEDVAALSGSVDVTLHRLALRFDRPAELDYEADATLRHVSMTL
                     KALPGALGIASGSMRFGRERARYEKLAVAMLDARALLSGTATIQGPRIELALDQGELG
                     EKAMRWALELAKAPPRLEPRTPLRFAARRIAWSPDEGLEADARIHFGGGPALGIALAW
                     RPELLDVRRLTIKDARSDALFSSRVADKRIEASFTGTLHGGSIAAMLKSTPADSGIAQ
                     GELRLTMDRKAPQNSIAQGKIQLQALDLTWLTGRKVQIERVSLSAAG"
BASE COUNT          411 a          937 c          948 g          416 t
ORIGIN      
        1 tgctgtcagc attatcgcct cgaaactgaa acggcgtctc gcgtggacgc tcggcggcat
       61 cgttgtggcc gccgccgctc tcatcgcgct cgcgccgctg ctcatcgatg cgcgcgccgt
      121 acaggccgag ctccagcgcc ggctctcgga cgcgctcggc ggccaggtga gctggcagga
      181 gctcgacgta cgcctgcttc ccgcgccgcg cggcgagctg cgcgcgcttc gcatagacgt
      241 ccccggaaag ctcgaagcgt ccgccgatgc ggtcgacgtg cacttgcgcc tgtggccgct
      301 gctgcgcggt caaccggaaa tctcctcgat cactgtggag cggccgcgca tccgggtcgc
      361 gcctcgcggt gatgcgccgg aggagcaaaa acccatcgac gtcatggcgc tttaccgcgc
      421 ggtgatggaa cccgcggcga acgcgctgca ggatttcgcg ccccggatgg agctccagtt
      481 gaacgaggcc gtcgtagcgg tgggcgagca gttcatgctg cgcgacctgc gcgcccgcgc
      541 gcgcaccgac agcaaaggcg tagagctcga gggttccagc gccgccacgc tttggcggcg
      601 gctgacggtc caggcgcgtt tcgaatacgc cgatctctcg gcgcgagcct ctgcggtggt
      661 cgagggcctc tcgttcgaca aggacattcc ggcggcggcg ctgcgcgccg atgtccgcac
      721 cgacggcagg agcgcgctcg aatgcgactt cgacggcagc gtcggaacgc tcgtccctgc
      781 ggtgaaaggc aagcttcttg ccgcagcggg caagccgctt gagctggcgg ccgacctcgg
      841 cggaatggac atgccacagg cgctcgcgct cgcgcgggca aagcaccttc ccctcgactt
      901 gatggagtcg gccgaaggaa agatctcgct cgcggcgact gcggtcgtgc aagagccctg
      961 gcgcgcggat gtcagccttg tgaaatccga tgccgccata aagctcgcag cgctgccgtg
     1021 gaagatttcg ccgcgcgcag cccgggtcgc ggttacgcgc gagcgggtaa ccgtgtcggg
     1081 actggaaggc ttcatcgaga agtccgcctt tagcgccgtg caggccgaca tcgacctcga
     1141 aacgaaccga ctcgcttcgg cctccggccg cgcgcggctc gagctcgcgc aatggctccc
     1201 gtggctgaag aagacgctgc cgctcgagga aatcgcttcg ctttccggcg aggccgatgt
     1261 ggcgctcgac cggctcgcac ttgccttcga tcgccccgcc gccgccgact tcgatgtgct
     1321 cgtcacgccg cgcaaggtgc gcgcggcggt caaggccctg cccgtaccgg tcagcgtaac
     1381 cagcggctcg gtgcgagccg caagcaggcg catcctgctc cagaacctcg cgggttcgct
     1441 cggcaactcg ccgttctcca gcctcactgc gcagatcgac ctcggcaaaa cgccgcgcgt
     1501 gtcttcggct tccgcgcgcg caagcctccg gcttgatcag tggttcccct ggctccggga
     1561 aaagctgccg ctccaggatg tcgcttcggt ctcggggcag gccgacgtca cgctcaaccg
     1621 gctcgcgctg cgcttcgatc ggcccgccga ggccgagttc gacgcggtcg tcacgccgcg
     1681 caaggtgagc gcggccgtca aggcgctgcc cgtcccggtg cgcgtgacgg gcggctcggt
     1741 ccgcgccggg cgctcgcgcg tgctgctccg tgacctcgaa ggttccctgg gagcttccgc
     1801 gttcggcaat ctcaacgcgc agatcgagct cgggaagacg ccgctcgtgt cgtcggcgtc
     1861 ggggcgtgcg agcgtccagc tcgagcagtg gtttccatgg ctgaaggggg cgttgctgtt
     1921 cgaggacgtg gcggcgctct cgggcagcgt cgacgtgacg ctgcaccgcc tggcgctgcg
     1981 cttcgatcgc ccggccgagc tcgactacga ggccgacgcc acgctgcgcc acgtgagcat
     2041 gacgctcaaa gcgctgcccg gcgcgctcgg catcgcgagc ggctcaatgc gcttcggacg
     2101 ggagcgcgcg cgctatgaaa agctggcggt ggcgatgctg gacgcccggg cgctgctctc
     2161 cggaacagcc acgatccagg ggccgcgaat cgagcttgcc ctcgaccagg gcgagctcgg
     2221 cgagaaggcc atgcgctggg cgctcgagct cgcgaaagcg ccgccgcgcc tcgagccgag
     2281 aacgccgctg cgcttcgcgg cgcggcgcat cgcgtggtcg ccggacgagg ggctggaagc
     2341 cgacgcgcgc atccacttcg gcggcggtcc cgcgctcggg atcgcgctag cgtggcggcc
     2401 cgagctgctg gatgtgcgcc gcctgacgat caaggacgcg cgcagcgatg cgctgttctc
     2461 gagcagggtc gccgacaagc gcatcgaggc aagcttcacc ggcacgctgc acggcggcag
     2521 catcgcagcg atgctgaaaa gcaccccggc ggactcgggc atcgcccagg gcgagctgcg
     2581 cttgacgatg gaccggaaag cgccgcagaa cagcatcgcg cagggaaaaa ttcagcttca
     2641 agccctcgac cttacgtggc tcaccggcag gaaagtgcag atcgagcgcg tgagcctcag
     2701 cgctgcgggc ga
//
LOCUS       DASUNT010000488         5103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_592452, whole genome shotgun sequence.
ACCESSION   DASUNT010000488 DASUNT010000000
VERSION     DASUNT010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592452"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="VFX94_09825"
     CDS             <1..863
                     /locus_tag="VFX94_09825"
                     /inference="COORDINATES: protein motif:HMM:NF024041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HEX5768519.1"
                     /translation="ALARTERKSGRGYKGFTIYASPEVTLEEDLKRRDLTINAMAKSE
                     DGTLIDPFGGKKDLDAGVLRHVSEAFAEDPVRILRVARFAARFGFSVHPSTLKLMQKM
                     VQAGEADFLVPERVWQEFSRGLAERRPELMFEVLQRAGLLAKAFPEIKAWPRQFSGSL
                     PVRFAVLAWPLGEEEVQALSERLRAPSEVRDVALAAVRSKEGLKQSTPDALLGVLKRA
                     DAFRRPERFAQLLEAARLADRGIDTARVERARAAAAAVDASAIAPSAHGADIGRLIDE
                     ARLRVIRELG"
     gene            complement(847..1032)
                     /locus_tag="VFX94_09830"
     CDS             complement(847..1032)
                     /locus_tag="VFX94_09830"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 family protein"
                     /protein_id="HEX5768520.1"
                     /translation="MLKWLVTVLVVLVIFAVVAPRFGAWRLPGDFSVPVRGRIYYIPL
                     ASTLVFSLLVWLISRVL"
     gene            1031..1648
                     /locus_tag="VFX94_09835"
     CDS             1031..1648
                     /locus_tag="VFX94_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5768521.1"
                     /translation="MIELYTWSTPNGRKVSIMLEECGLPYNVHKINIGTNREQFAPEY
                     LKINPNGKIPSIVDPDGPDGKPIAMMESGAILIYLADKTGTFFPKKDKYEVLQWLMFQ
                     MGGVGPMFGQAHHFMRAKKDEIAYGSERYGNEAKRLYGVMDKRLSENDYFAGEYSIAD
                     IAIYPWVARHEWHRVDLSAFPSVRRWYDKVGARPGVVKGMAVPQV"
     gene            complement(1658..2308)
                     /locus_tag="VFX94_09840"
     CDS             complement(1658..2308)
                     /locus_tag="VFX94_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768522.1"
                     /translation="MRALPAAILLFAFPAFADELSQAKRRWEASPHGPMLERILPPTF
                     DVARLPERESPAARLVATYCVQCHNLPNPAMHHAGKWPGIVQRMVLRMKGKGNMGTLM
                     AEMMAGVKAPGAEDTKAIVTYLQRHSQKPLDPARYPEVNQRSGEAFRLACNQCHVLPD
                     PQRYTAAEWPSVVARMQENMEWMNRVVGSKPVPGEPQLAIREINAFLARHARKPGG"
     gene            complement(2312..3343)
                     /locus_tag="VFX94_09845"
     CDS             complement(2312..3343)
                     /locus_tag="VFX94_09845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5768523.1"
                     /translation="MLQELRAHIAAAGGWITFARYMELALRAYYGRGERQFGAQGDFV
                     TAPELGKLFGRTLARQLAQLEGPILEFGAGSGALAEVLTSEHDGEYLILETSAALRSR
                     QAARLGGKVKFLDRLPDIFRGVVIANEVLDAMPVHAVGWRKEGIVERGVAFSDELVWE
                     EKPATGAVLEAAKEIRVPKPYDSEIGLAARAWMRSVGERLESGAIFVIDYGFPSAEYY
                     HPQRSMGTLMCHARHRTHGDPFEHPGEQDITAHVDFSALAQAANEAGLDLLGYTAQAQ
                     FLVNCGITEVLAQANVENALRYAPIAAEAQVLLSPNEMGELFKVLAVGRGIGSALLGF
                     SHGDRSHRL"
     gene            3375..4121
                     /locus_tag="VFX94_09850"
     CDS             3375..4121
                     /locus_tag="VFX94_09850"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF006598.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HEX5768524.1"
                     /translation="MDDSLAGKVALVTGGGRRVGAAIARRLHAAGASVLLHYRDSEAD
                     ATRLAGELNATRPKSAAKVKAELLAPIAPRALVSAALESFGRLDLLVNNASTFFPVAV
                     GAMEASHWEELIGSNLRAPLFISQQAADELAKNEGSIVNIVDIHAERPLKGYALYSIA
                     KAGLAAMTRSLARELAPRVRVNGVAPGAIAWPEDGQFADDERARILATTPLARVGSPQ
                     DVARAVHFLACAAYVTGEIIAVDGGRSIFI"
     gene            4131..4448
                     /gene="sugE"
                     /locus_tag="VFX94_09855"
     CDS             4131..4448
                     /gene="sugE"
                     /locus_tag="VFX94_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEX5768525.1"
                     /translation="MAWLWLTLAGLLEVVWAIGLKYTDGFTRLTPSVITVAAMIGSVY
                     FLALAVRAIPIGTGYAVWTGIGAVGVAILGMVLFGEPKTALRIGSILLIVMGIAGLKL
                     VTP"
     gene            4466..>5103
                     /gene="ttcA"
                     /locus_tag="VFX94_09860"
     CDS             4466..>5103
                     /gene="ttcA"
                     /locus_tag="VFX94_09860"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEX5768526.1"
                     /translation="MAAQPIRLHRDPRKAEYELGKLAKRLRRQAGEAIADFKMIEAGD
                     RVMVCLSGGKDSHGLLDVLLSLQEKAPLAFELIAVNLDQKQPGFPARVLPEYLRSRGV
                     EFRIVEQDTYSVVKRVIPEGQTMCSLCSRLRRGVLYRVAAELGATKIALGHHRDDILA
                     TFFLNLFHGGQLKAMPAKLRTDDGRNVVIRPLAYVAEADLAAYAEAKRFPIIP"
BASE COUNT          861 a         1704 c         1685 g          853 t
ORIGIN      
        1 acgcgctcgc gcgtacggag cgcaaaagcg gccgcggcta taagggcttc acgatctacg
       61 cctcgcctga agtgacgctg gaagaagacc taaagcgccg cgatctcacc atcaacgcga
      121 tggcgaagtc cgaagacggc acgctgatcg atcccttcgg cggcaagaag gacctggacg
      181 cgggcgtgct gcgacacgtg agcgaggcgt tcgccgaaga cccggtacgg attcttcgcg
      241 tcgcgcgctt cgcggcgcgc ttcggttttt ccgtccatcc ctcgaccctg aagctcatgc
      301 agaagatggt gcaggcgggc gaagccgatt tcctggtgcc cgagcgggta tggcaggagt
      361 tttcgcgggg gctcgccgag cggcgccccg agctcatgtt cgaggtactg cagcgcgccg
      421 ggcttcttgc caaggcgttt cccgaaatta aggcttggcc gcggcagttc tccggctcgc
      481 ttccggtgcg cttcgccgtg ctcgcatggc cgctcgggga agaagaagtg caggcgttgt
      541 ccgagcggct gcgcgcgccg agcgaagtgc gggatgtcgc ccttgccgca gttcgcagca
      601 aagaagggct gaagcaatcg acgcccgacg cgcttctcgg cgtgcttaaa cgcgccgatg
      661 ccttccgccg cccggagcgg ttcgcgcagt tgctcgaggc ggcgcggctc gcagaccgcg
      721 gcatcgacac cgcgcgcgtc gagcgcgctc gtgcggcggc agccgccgtg gatgcgtccg
      781 cgatcgcccc ctcggctcac ggcgcggaca tcggccggct gatcgacgag gcgcggctca
      841 gggtgatcag agaactcggc tgatgagcca gaccagcagc gaaaagacga gcgtgctggc
      901 gagcggaatg tagtagattc gtccgcgcac cgggacgctg aaatcccccg gcaggcgcca
      961 ggcgccgaag cgcggcgcga ccacggcgaa aatcaccaac accaccagca ccgtcacgag
     1021 ccacttcaac atgatcgaac tctatacctg gagcacacca aacggacgca aggtttccat
     1081 catgctcgag gagtgcggcc tgccgtacaa cgtgcacaag atcaacatcg gcaccaaccg
     1141 cgagcaattc gcgcccgagt acctcaagat caacccgaac ggcaagattc cgtcgatcgt
     1201 cgacccggac ggtcccgacg gcaagccgat cgcgatgatg gagtctggcg cgatcctcat
     1261 ctacctcgcg gacaagaccg ggacgttctt tccgaagaag gacaagtacg aagtcctgca
     1321 gtggctcatg ttccagatgg gcggggttgg gccgatgttc gggcaggcgc accacttcat
     1381 gcgggccaag aaggatgaga ttgcctatgg aagcgagcgc tacgggaacg aagccaaacg
     1441 gctgtacggc gtcatggaca agcggctttc cgaaaacgac tactttgccg gcgagtattc
     1501 cattgcggat atcgcgatct atccctgggt cgcgcgccac gagtggcacc gtgtggacct
     1561 ctccgcgttc ccaagcgtga ggcgctggta cgacaaggtc ggcgcgcgtc cgggcgtcgt
     1621 caagggcatg gcggtgcccc aggtctgagc cgcagcgtca gccgcccggc ttgcgcgcgt
     1681 gcctcgccag gaaggcgttg atctcgcgga tcgcgagctg cggctcgccc gggacgggct
     1741 tcgatccgac cacgcggttc atccactcca tgttttcctg catgcgcgcg accacgctcg
     1801 gccactccgc tgcggtgtag cgctgcgggt cgggaagcac gtggcattgg ttgcaggcga
     1861 ggcggaaagc ctcgccggaa cgctggttca cctcggggta gcgcgccggg tcaagcggct
     1921 tctgtgaatg gcgctgcagg taggtgacga tcgccttggt gtcctcggcg cctggcgcct
     1981 tgactccggc catcatctcg gccatcagcg ttcccatgtt ccccttgccc ttcatgcgga
     2041 gcaccatgcg ctgcacgatg cccggccact tcccggcgtg gtgcatcgcg gggttgggga
     2101 ggttgtggca ctgcacgcag tacgtcgcca ccagcctcgc tgccggtgat tcccgctccg
     2161 gcagacgcgc gacatcgaaa gtcggcggca ggatgcgctc gagcatcggc ccgtgcgggc
     2221 ttgcttccca gcgcctcttt gcttgggaga gttcgtccgc gaacgcgggg aatgcgaaga
     2281 gcagaattgc ggctggcagg gcgcgcatgc cctacagcct atgggaacga tccccgtgtg
     2341 aaaaccccaa aagcgcagac cctatgccgc ggccgacggc gaggaccttg aaaagctcgc
     2401 ccatctcgtt tggcgagagc aggacctgcg cctcagcggc gatcggcgca tagcgtagcg
     2461 cgttctccac gttcgcctgc gccagcacct cggtgatgcc gcagttcaca aggaactgcg
     2521 cttgcgccgt gtagccgagc aggtcgagcc ccgcctcgtt cgccgcctgc gcgagcgcgc
     2581 tgaagtcgac gtgcgcagta atgtcctgct cgcccggatg ctcgaacggg tcgccgtgtg
     2641 tgcgatggcg cgcgtggcac atcaacgtgc ccatcgagcg ctgcggatgg tagtactcgg
     2701 ccgacggaaa tccgtagtcg atgacgaaga tcgccccgga ctcgagccgc tcgccaacgc
     2761 tgcgcatcca ggcccgggcg gccagcccga tctcgctgtc gtacggtttg ggaacgcgga
     2821 tctctttcgc ggcctcgagc accgcgccgg tggccggctt ctcctcccac acaagctcgt
     2881 cggagaaggc cacgccgcgc tccacgattc cttctttgcg ccaaccgacc gcgtgcaccg
     2941 gcatggcgtc cagaacttcg ttcgcgatga cgacgccgcg aaaaatgtcc ggcagccggt
     3001 ccaggaattt cactttgccg ccgagccgcg ccgcctggcg cgagcgcagc gcagcgctcg
     3061 tctcgaggat caggtattcc ccgtcgtgct cgctcgtgag cacctcggcg agcgcgccac
     3121 tgccggcgcc gaactcgaga atcggccctt cgagctgcgc gagctgccgg gcgagcgtgc
     3181 ggccgaagag cttccccagc tcgggagcgg tgacgaaatc gccctgcgcg ccgaactggc
     3241 gctcgccgcg gccgtagtac gcgcgaagcg cgagctccat gtagcgggcg aacgtgatcc
     3301 acccgccggc ggccgcgatg tgcgcgcgca gctcctgaag caccggcgca atctaaccta
     3361 gaatcagcgc cttcatggac gattcgctgg ctggcaaggt ggccctcgtc accggcggcg
     3421 ggcggcgcgt cggcgcggcg atcgcgcggc ggctgcacgc cgcgggagcg agcgtcttgc
     3481 tgcattaccg cgactcggag gccgacgcca cccgcctcgc aggcgagctc aatgcaacgc
     3541 ggccgaaaag cgccgcgaag gtgaaggctg agctgcttgc cccgatcgcc ccgcgggcgc
     3601 tcgtcagcgc agcgctcgag agcttcggac ggctcgatct tctcgtgaac aacgcctcga
     3661 ccttcttccc ggtagcggtg ggtgctatgg aagcgtctca ctgggaggag ctcatcggct
     3721 cgaacctgcg cgcgccgctt ttcatctcgc aacaggcggc cgacgagctc gcgaagaacg
     3781 aaggctccat cgtgaacatc gtggacatcc atgccgaacg gcccctcaag ggctatgcgc
     3841 tctacagcat cgcaaaggcg ggcctcgctg ccatgacgcg ctcactcgcg cgcgagctcg
     3901 cgccgcgcgt gcgcgtgaac ggcgtcgccc ccggcgccat tgcctggccc gaggacgggc
     3961 agtttgcgga cgacgaacgc gcccgcatac tcgccacgac accgcttgcg cgcgtcggat
     4021 cgccgcaaga cgtggcgcgc gcggtgcact tcctcgcctg tgccgcctac gtgaccggag
     4081 agatcatcgc cgtggacggc ggccggtcca ttttcatctg aggaaaccac atggcctggc
     4141 tgtggctcac gctcgccggt ctgctcgaag tcgtctgggc gatcggcttg aagtacaccg
     4201 acggcttcac gcggctcacg ccttcggtca tcaccgtggc ggcgatgatc ggcagcgtct
     4261 actttctcgc gctcgcggtg cgcgccatcc cgatcggcac cggctacgcg gtctggaccg
     4321 gtatcggcgc ggttggtgtc gcgatcctcg gcatggtgct cttcggcgag ccgaagacgg
     4381 cgcttcgcat cggttcgatc ctcctcatcg taatgggcat cgccgggctc aagctggtaa
     4441 cgccttaggt aaactcgcgt tccttatggc cgcccaaccg atccgcctgc atcgcgaccc
     4501 gcgcaaggcc gagtacgagc tcggcaagct ggcgaagcgg ctgcgccgcc aggcgggcga
     4561 ggcgatcgcg gacttcaaga tgattgaggc gggcgaccgg gtaatggtct gcctgtccgg
     4621 cggcaaggac agccatgggc tcctcgatgt gctgctctcg ctgcaggaaa aggcgccctt
     4681 agcgttcgag ctcattgcgg tcaatctgga ccagaagcag cccgggttcc cggcgcgggt
     4741 gctacccgaa tacctgcgct cgcgaggcgt cgagttccgt atcgtcgagc aggacaccta
     4801 ctcggtggtg aagcgggtga tcccggaggg gcaaaccatg tgctcgctct gctcgcgctt
     4861 gcgacgtggc gtgttatacc gcgtggccgc cgagctcggc gcgacgaaga tcgcgctcgg
     4921 ccatcaccgc gacgacattc tcgccacctt tttcctcaac ctcttccacg gcgggcagct
     4981 gaaagcgatg ccggcgaagc tgcgcacgga tgacggccgg aatgtcgtaa tccggcccct
     5041 ggcgtacgtc gccgaggccg atctcgcggc ctacgccgaa gcgaagcgtt tcccgatcat
     5101 tcc
//
LOCUS       DASUNT010000489         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1207391, whole genome shotgun sequence.
ACCESSION   DASUNT010000489 DASUNT010000000
VERSION     DASUNT010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207391"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VFX94_09865"
     CDS             complement(<1..557)
                     /locus_tag="VFX94_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5768527.1"
                     /translation="MTAVALTPPAAASDALPAILLVEDEPTTRLMTARQLKRAGYEVE
                     AVANGVEALARLRDRFFPLLLTDWEMPEMDGLALCRAVRESSLEGYVYTILLTARDSK
                     AHIIEGLQAGADDYLTKPVDDSELMARLNTGRRILELERSLKAANHEKHLLSITDPLT
                     ATFNRRYLMEQLQKEIERARRYSRPL"
     gene            complement(688..1581)
                     /locus_tag="VFX94_09870"
     CDS             complement(688..1581)
                     /locus_tag="VFX94_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX5768528.1"
                     /translation="MKAACAALLAATLFATQAVADDEEMIDKRLAADPAGEVEVSNVA
                     GSVNVSGWDRAEVHVSGELGSGVERLVFESSGKRTVVKVMLKKGYHRGGNGETDLNIR
                     VPKGSRLDVNTVSAEIIAQDVTGAQRLQSVSGDISTETSAEADLKTVSGTISLRGNGQ
                     SSQLDLTTVSGDAVVTRIAGEVTVSTVSGSLELSLDKLTRAKLRTTSGDLSVSARLDP
                     NARIDAETISGQLTLDFKGGVDAQIEAESFSGDIESCSGPKAKSTQEYGPGSELRYTE
                     GKGTGRISVSSLSGDINLCTR"
     gene            complement(1578..>2236)
                     /locus_tag="VFX94_09875"
     CDS             complement(1578..>2236)
                     /locus_tag="VFX94_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768529.1"
                     /translation="EETDVSNDMTGKNGHDDALLRAVRRMPQDIAPRRDLWSGIEAAI
                     TAAPSGVASLDAARAKKAWTSRLSWPYALAAGVGCMALGALLTFGLLRGQAPISQQAT
                     NLPAPAPPLEQQPAVRYSDASFGGYEALGPEYEQARAQLAIGLAQRLDRLPPEAQQKV
                     ERNLAEIRRSLREINLALALDPDSTLLQQLLLSTYQSELAMLANVNQMAGEIPARSET
                     "
BASE COUNT          314 a          763 c          746 g          413 t
ORIGIN      
        1 agaggtcgcg agtagcggcg cgcgcgctcg atctccttct gcagctgctc catcaggtag
       61 cggcggttga aggtcgccgt gagcggatcg gtgatggaga gcaggtgctt ctcgtggttc
      121 gcggccttga gcgaacgctc cagctcgaga atgcgccgcc ccgtgttgag gcgcgccatc
      181 agctcgctgt catcgacggg cttggtgaga tagtcgtccg cgcccgcctg caggccctcg
      241 atgatgtgcg ccttggaatc gcgcgctgtg agcaggatcg tatagacgta gccttccagg
      301 ctgctctcgc ggaccgcgcg gcacagcgcc aggccgtcca tctcgggcat ttcccaatca
      361 gtgagcagca gcgggaagaa gcgatcgcgc agacgtgcga gcgcctcgac accgttcgcc
      421 acggcctcga cctcgtagcc cgcgcgcttg agctgacgcg ccgtcatcag gcgcgtggtc
      481 ggctcgtcct cgaccagcag gatggcgggg agcgcgtcgg atgccgcggc gggcggcgtg
      541 agggcaacgg ctgtcacgga acgggcctga agcgagccga ccctgtcggc tcttcgtggc
      601 ccctctatcg gcacgacgcc gcggaacttg agtccgcggc gttcggcgat cggcttacaa
      661 gcccttgtta ctcaaggctt gtgcgcgtca gcgggtgcag agattgatgt ccccgctcag
      721 gctggagacc gagatccggc cggtgccctt gccctccgtg tagcgcagct cgcttcccgg
      781 gccatattcc tgggtgctct tggccttcgg acccgagcag ctctcgatgt cgcccgagaa
      841 gctctcggcc tcgatctgcg cgtccacgcc acccttgaag tcgagcgtca gctggccgct
      901 gatggtctcg gcgtcgatgc gcgcgttggg gtccaggcgc gcgctcacgc tgagatcccc
      961 ggaagtcgtg cgcagcttgg cgcgcgtcag cttgtcgagc gacagctcca gcgagccgct
     1021 gaccgtggac accgtgactt cgccggcgat gcgcgtcacc accgcatcgc cgctcaccgt
     1081 cgtgaggtcg agctgcgagg actggccgtt gccgcgcagc gaaatcgtgc cgctcaccgt
     1141 cttcagatcc gcttcggcgg aggtctcggt ggagatgtct cccgacaccg attgcaggcg
     1201 ctgcgcgccg gtcacgtcct gggcgatgat ctcggcgctc accgtgttca catccaaccg
     1261 gctgcccttg ggcacgcgaa tgttcagatc cgtttcgccg ttgccgccgc ggtggtagcc
     1321 cttcttgagc atcaccttca ccaccgtgcg cttgccgctg ctctcgaaca ccaggcgctc
     1381 cacgcccgat ccgagctcgc cgctgacgtg cacttcggcg cggtcccagc cactgacgtt
     1441 gacgcttccc gcgacgttgg acacttcgac ttctcccgcg ggatccgcgg ccagtcgctt
     1501 gtcgatcatt tcttcatcgt ccgccacggc ttgcgtggca aacagggtgg ccgccagcag
     1561 cgcggcgcat gcagctttca tgtttccgac ctcgcgggaa tctcgcccgc catctgattg
     1621 acgttggcga gcatggcgag ctcgctctgg taagtgctca gcaacagctg ctgcaacagg
     1681 gtgctgtccg gatcgagcgc gagcgcaagg ttgatctcgc gcagggagcg gcgaatctcg
     1741 gcgagattgc gctccacttt ctgttgggcc tcgggcggca ggcggtcgag ccgctgcgcg
     1801 aggccgatcg cgagctgcgc ccgcgcctgt tcgtattcgg gccccagggc ctcgtagccg
     1861 ccgaagctcg catcggagta acgcacggct ggctgctgct cgagcggcgg cgccggtgcg
     1921 ggcagattcg tggcctgctg cgagatcgga gcctggccgc gcagcagccc gaacgtgagc
     1981 agcgcgccga gcgccatgca acctacgccc gcggcgagcg catagggcca gctcagccgg
     2041 ctcgtccacg ctttcttcgc acgcgcggca tcgagcgagg cgacgccact cggcgccgcg
     2101 gtgatggcag cctcgatccc gctccacaga tcgcggcgcg gggcgatgtc ctgcggcata
     2161 cggcgcaccg cgcgcagcag cgcgtcgtcg tgtccgttct tgcctgtcat gtcgttgctc
     2221 acgtcagttt cttccg
//
LOCUS       DASUNT010000490         3139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_768450, whole genome shotgun sequence.
ACCESSION   DASUNT010000490 DASUNT010000000
VERSION     DASUNT010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768450"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFX94_09880"
     CDS             complement(<1..261)
                     /locus_tag="VFX94_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5768530.1"
                     /translation="MNRRHFLAAVIAASLSSAALAQDYGKPGGPVTVVVGYQPYYTQS
                     WSGVVMRGKKFYEKYLPKGSNVEFQIGLQGAIIVNNMLAGKQH"
     gene            complement(290..1018)
                     /locus_tag="VFX94_09885"
     CDS             complement(290..1018)
                     /locus_tag="VFX94_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5768531.1"
                     /translation="MAKRRVCRVADVPENTLKEFEAEGLKLVVANAGGEFVGFQAMCP
                     HQDVPLCEGLFDGQTLTCHMHLWQWDVRTGAPQGIAETPLERYPLVREGDALYLGGES
                     GALDVGELFNGLNSSLIEKLSALARKESHAAGATLYRPGDAADDFFVLDSGRIEFLLG
                     RGDRAAPGGFMLKKGETFGWAALLDATPARIASARCLEDSSVLRINGRAALRVLEGDP
                     AAGFTVMRRLAALIARYLASSGAK"
     gene            complement(1018..2022)
                     /locus_tag="VFX94_09890"
     CDS             complement(1018..2022)
                     /locus_tag="VFX94_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5768532.1"
                     /translation="MNARNRGFEFDASPGESVLYAGLRAGIDLPYECATGTCGTCKAK
                     LLSGQVSDRWPGAPGKKYVKQAGEFLMCQCAADGPLTLEVSKFVYPVEPGACVPQPCS
                     GTVRKVRVLTHDVMGFALELERPRDFDAGQFALLAAGGVEGYRAYSMCNYERAARKLD
                     FVVKKKPDGGFSDWLFGGNREGARLEAFGPLGKATFDPALGKNLLCIAGGSGIAGMMS
                     ILARAEAADYFSRHKAWVFFGVRTMKDAFFLEQLARSKAQVTIALSEEEVPSRHEWPN
                     LRFEKGLVHEVAKRAMAGKYQNVRAYVAGPPPAVDAAIRVLLLDARLTTDNIRYDKFS
                     "
     gene            complement(2038..2499)
                     /locus_tag="VFX94_09895"
     CDS             complement(2038..2499)
                     /locus_tag="VFX94_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEX5768533.1"
                     /translation="MNIEQRKAVEEVVYRSCLALDARDYKSFLELCDPEFRYTISTYS
                     PEIRREMVWLDHDRKGMETLFTNLPRHNSDHSPLTRHATVYTVTENGASAEVVSAIQV
                     FKTELDGGATELFAVGRLHDTVKLDGANPRLAKRVVRLETRLLGYGYHIPF"
     gene            complement(2501..>3139)
                     /locus_tag="VFX94_09900"
     CDS             complement(2501..>3139)
                     /locus_tag="VFX94_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RHO alpha subunit C-terminal catalytic
                     domain-containing protein"
                     /protein_id="HEX5768534.1"
                     /translation="KLWHDTNSEFYHDYLHYFNRITGMMQPGYFYRKYTGFPNGHATV
                     GSMQIQYDKYEGSKERAVGWPGLARGGWYMVDVFPGMTYNLRTSVLRIDTAIPLGPNE
                     VLIEFRGLGLKSDSAEDRRQRVFDHNMIWGPFGRNLHEDLLGVHGQGRAMRRGTDSRW
                     VLQGREENNTIHDEVGMRHYYAEWSRRMGRPAYDPYREGRPERTLQNARAAA"
BASE COUNT          495 a         1064 c         1024 g          556 t
ORIGIN      
        1 gtgctgcttg ccggccagca tgttgttgac gatgatcgcg ccctgcaggc cgatctggaa
       61 ctcgacgttc gagcccttcg gcaggtactt ctcgtagaac ttcttgccgc gcatcaccac
      121 gccggaccac gactgggtgt agtacggctg gtagccgacc acgacggtga ccggtccgcc
      181 cggcttgccg tagtcctgcg cgagtgcagc gctactcagc gatgccgcta ttaccgctgc
      241 caggaaatgc cgcctgttca tgactgtctc ctctctcctc ggttgtcgtt cactttgctc
      301 cggatgatgc gagataacgc gcgatcagcg ccgccagccg gcgcatgacc gtaaagccgg
      361 ccgccgggtc gccctccagc acccgcaggg ccgcccggcc gttgatgcgc agcaccgacg
      421 aatcctccag gcagcgcgcg ctcgcgatgc gcgccggcgt agcgtcgagc agcgccgccc
      481 agccgaatgt ttcgcccttc ttcagcatga agccgccggg cgcggcgcgg tcgccgcgcc
      541 cgagcaggaa ctcgatgcgc ccggaatcga gcacgaagaa atcgtccgcc gcatcgccgg
      601 gacggtagag cgtcgcgccg gcggcatggc tttcctttct tgccagcgcg gaaagctttt
      661 cgatcaggga agaattcaag ccgttgaaca gctcgccgac gtccagcgcg cccgactcgc
      721 cgcccaggta aagcgcgtcg ccttcgcgca ccagcgggta gcgctcgagc ggcgtctcgg
      781 cgatgccttg cggcgcgccg gtgcgcacgt cccactgcca gaggtgcatg tggcaggtga
      841 gcgtctggcc gtcgaacagc ccttcgcaga gcggcacgtc ctggtgcggg cacatcgcct
      901 ggaagccgac gaactcgccg cccgcgttcg ccaccacgag cttgaggccc tcggcctcga
      961 actccttgag ggtattctcg ggaacgtcgg cgacccggca gaccctgcgt ttcgccatca
     1021 gctgaatttg tcgtagcgga tgttgtcggt ggtgagccgc gcgtcgagca gcagcacgcg
     1081 gatcgcggcg tccaccgcgg gcggcggccc ggccacgtag gcgcgcacgt tctggtactt
     1141 gccggccatg gcgcgcttcg ccacctcgtg caccaggccc ttctcgaacc gcaggttcgg
     1201 ccactcgtgg cgcgatggca cctcttcctc ggagagcgcg atcgtcactt gcgccttgga
     1261 tcgagccagc tgctcgagaa aaaaagcatc cttcatcgtg cgcacgccga agaacaccca
     1321 ggccttgtgc cgggaaaagt agtccgcggc ctcggcgcgc gcgagaatcg acatcatgcc
     1381 ggcaatgccg gagccgccgg cgatgcacag taggttcttg cccagcgccg ggtcgaacgt
     1441 cgccttgccg agcggcccga aagcttccaa acgcgccccc tcgcggttgc cgccgaacag
     1501 ccagtccgag aagccgccgt cgggtttttt cttcaccacg aagtcgagct tgcgcgcggc
     1561 gcgctcatag ttgcacatcg agtaggcgcg gtagccctcg acgccgccgg cggcgagcag
     1621 cgcgaactgg ccggcgtcga aatcgcgcgg ccgctcgagc tccagcgcaa agcccattac
     1681 gtcgtgcgtc agcacgcgca ccttgcgcac cgtgccgctg cagggttgcg gcacgcaggc
     1741 accgggctct acggggtaga cgaacttcga gacttcgagg gtcaacggcc catccgccgc
     1801 gcactggcac atcaggaact cccccgcctg cttcacgtac ttcttccccg gcgcgccggg
     1861 ccagcggtcg ctcacctggc ccgacagcag cttggccttg cacgtgccgc aggtgccggt
     1921 tgcgcactcg taaggcaggt cgatgccggc gcgcaggccg gcgtagagca cgctctcgcc
     1981 gggcgaggcg tcgaactcga agccgcggtt gcgcgcgttc acctggatgc gcattcgcta
     2041 gaacgggatg tggtagccat agccgagaag ccgcgtttcc aggcgcacga cgcgctttgc
     2101 gagccgcggg ttggcgccat cgagcttgac cgtgtcgtgc aagcggccga cggcaaatag
     2161 ctcggtcgcg ccgccgtcga gctctgtctt gaagacctgg atggcggaaa cgacctcggc
     2221 cgaggcgccg ttttcggtca cggtgtacac cgtcgcgtgc cgggtgagcg gcgagtggtc
     2281 gctgttgtgg cgcggcaagt tcgtgaacag cgtctccatt cccttgcggt catggtcgag
     2341 ccataccatc tcgcgccgga tctcgggact gtaggtggag atcgtgtagc ggaattccgg
     2401 atcgcagagc tccagaaacg acttgtagtc cctcgcgtcg agcgcgaggc acgagcggta
     2461 gacgacctcc tccaccgcct ttctttgctc gatgttcatc tcaggcggcg gcgcgcgcgt
     2521 tctggagcgt gcgctccggc ctgccctcgc ggtaagggtc gtacgccggg cgtcccatgc
     2581 gccgcgacca ctcggcgtag tagtggcgca tgccgacttc atcgtgaatg gtgttgttct
     2641 cctcgcgccc ctggagcacc cagcggctgt cggtgccgcg gcgcatcgcg cggccctggc
     2701 cgtgcacgcc gagcaggtcc tcgtgcaggt tgcggccgaa cggaccccag atcatgttgt
     2761 ggtcgaacac gcgctggcga cgatcttccg cgctgtcgct cttgagcccc aggccacgga
     2821 attcgatcag cacctcgttc ggcccgaggg gaatcgcggt gtcgatgcgc agcaccgagg
     2881 tgcgcaggtt gtaggtcatc cccggaaata catccaccat gtaccagccg ccgcgcgcaa
     2941 ggcccggcca gccgaccgcg cgctccttcg agccttcgta cttgtcgtat tgaatctgca
     3001 tcgagccgac cgtggcgtgg ccgttcggga agccggtgta cttccggtaa aagtagccgg
     3061 gctgcatcat gccggtgatg cggttaaagt agtgcaggta gtcgtggtag aactcgctgt
     3121 tcgtgtcgtg ccacagctt
//
LOCUS       DASUNT010000491         4965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_943354, whole genome shotgun sequence.
ACCESSION   DASUNT010000491 DASUNT010000000
VERSION     DASUNT010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943354"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /gene="otnC"
                     /locus_tag="VFX94_09905"
     CDS             complement(<1..536)
                     /gene="otnC"
                     /locus_tag="VFX94_09905"
                     /EC_number="4.1.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate 4-phosphate decarboxylase"
                     /protein_id="HEX5768535.1"
                     /translation="MNEAILREQLVMFGESLHARGYAHGSSGNLSARLPDGLLITPTN
                     SCLGRLDPARISKLNGKGEHLSGDAPSKEAFLHLAMYQERPSAQAIVHLHCTHCVAVS
                     CLHGFAGLPPITAYYVMRIRELALVPYYRPGDRALAEAVREKAKKHHAVLLANHGPVV
                     AGKSLEDAVVNAEELEETA"
     gene            complement(533..1729)
                     /gene="otnK"
                     /locus_tag="VFX94_09910"
     CDS             complement(533..1729)
                     /gene="otnK"
                     /locus_tag="VFX94_09910"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HEX5768536.1"
                     /translation="MRLACIADDLTGATDLGVTLVREGLSVIQVNGVPDRSLAVPPAD
                     CVVVALKSRTNPAAEAVAWSLASLDWLRAHKAERIYFKYCSTFDSTARGNIGPVADAL
                     LDALAERFSVATPAYPRNGRTVYKGNLFVGDVPLAESGMKDHPLTPMRDSNLVRVLAA
                     QTPHGVGLVPFEVMDAGPRAIRKKFEELQKTARYAILDATREEHLVAAGEACRDMKLT
                     TGGAGLAMGIARALGKRGPAAQALPRLGGPAVVLAGSCSQATLGQIAAWTAPALRLDP
                     HKLDAAAAVDWAARNLDSTPLLYASAPAAEIAQGRGEAIEQAFRDIARALAAKGVRKF
                     VVAGGETSGAVVEALGVRALAIGPEIDPGVPWTAAVGGEPYLLALKSGNFGSREFFRK
                     ALEMIR"
     gene            1824..3089
                     /locus_tag="VFX94_09915"
     CDS             1824..3089
                     /locus_tag="VFX94_09915"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5768537.1"
                     /translation="MRFVKFLIATAALVLCVQAQAQVKLTLWSHWAAEKIKRDYVEDA
                     IKRFEAANPGVKVEAQWYEKTALYAALKTALRAGTAPDIFYAEPDQVEYMENALLLDL
                     SALNWAAVEPWAKEIWSYKGKPYGLPLEAWTVEVYYNKKTLSDLGVEVPGNLQLDAAT
                     LGDVAKKARAKNMTPMSLGVGDRPFPGAHLTHEALLKKLGVQEYDNLLKGKLSWSDPR
                     VVETLRVVRGWVEAGLLPTTFTTLKLGEAHTYFHTNPGAVMFLNGSWYTSRAFNPPDK
                     GGQPPNFPLGVMKFPTVPGAACNECRTIAVGGSYVANAATRNPKQAIAFLNSFATPEM
                     ANRWLENVLVQTGIKADYSKIAGPHAGYFKELGATSQGAKYYFGLPIQVMQGKQKEVF
                     TQVFNNAFPAGTISVDEAVKQMNAAYGKH"
     gene            3086..3985
                     /locus_tag="VFX94_09920"
     CDS             3086..3985
                     /locus_tag="VFX94_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX5768538.1"
                     /translation="MTARASQDRQPWGAIVAFLLPAFTVYVALTAYPVLRTFWNSFHK
                     VLPRSEEYIGAANYAALVQDELFWRAVRNTFTWACISPLVEVTIALLLALALYARVPG
                     MRFFRIAWFTPVLMSYVVVGIIWVWIYNYDWGAANALLRLLGLGAFVQAWIGDPKLAL
                     PSLIFVTTWMWTGFNMVVLLAALHSLPSEVIEAAELDNCGWGAKLWYVIVPLIKPTIL
                     NLLVLSFIGKMKIFDLVWITTKGGPLWSTETVSTYLYKRAFEWSTFDLGYPSTIAAVW
                     FVIVLAAVLGLTWLLRTRGRLEY"
     gene            4004..4795
                     /locus_tag="VFX94_09925"
     CDS             4004..4795
                     /locus_tag="VFX94_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX5768539.1"
                     /translation="MIILALYTAFAAGPFLWVATMSVRTTSEIALDHYAWPEQWHWEK
                     FPQAWFNSNYSVYFENSLLVVLAAVAVLTVVGAMAAHCLARYRWRTNRLFYFILFSAI
                     IFPPQITIIALFQILVQYGLYNSLLGLTAVYIAVQLPLTVYILESFFARIPSDLFDAA
                     RIDGYSEWQIFWKVSLPIALPAIATTVILNTILLWNEFLYAVVLITDEAKRTLPLGIQ
                     RFMGDQLEDVGMIATGLMIAIIPVVLIYAFFSEKLIQGMTAGAVK"
     gene            4795..>4965
                     /locus_tag="VFX94_09930"
     CDS             4795..>4965
                     /locus_tag="VFX94_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011356658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5768540.1"
                     /translation="MATVTLHALSKRYPGSAHLAVDAVDLDVAHGEFMVLLGPSGCGK
                     STTLRMVAGLESI"
BASE COUNT          842 a         1632 c         1606 g          885 t
ORIGIN      
        1 gccgtctctt ccagctcctc ggcgttgacc accgcatcct cgagcgactt cccggcgacg
       61 acgggaccgt ggttcgcgag cagcacggcg tggtgcttct tcgccttctc gcgcaccgcc
      121 tcggcgagcg cgcggtcgcc cgggcggtag tacggaacga gcgccagctc gcggatgcgc
      181 ataacgtagt acgcggtgat gggcggcagc ccggcgaagc cgtgcaggca cgacaccgcg
      241 acgcagtgcg tgcagtgcag gtgcacgatg gcttgcgcgg agggccgttc ctggtacatc
      301 gccagatgca ggaacgcttc cttggagggc gcgtcgcccg agaggtgctc accttttcca
      361 ttcagcttgg aaatgcgcgc cggatcaagg cgtccaaggc aggaattcgt cggcgtgatg
      421 agcagaccgt cgggcagccg cgcgctaagg ttgcccgagc tgccgtgcgc atagccgcgc
      481 gcatgaagcg attcgccgaa catcacgagc tgctcgcgca aaatggcttc gttcaccgga
      541 tcatctccaa cgccttgcgg aagaattcgc ggctgccgaa gttgcccgac ttgagcgcaa
      601 gcaggtacgg ctcgccgccg accgcggcgg tccagggcac gcctgggtcg atctcggggc
      661 cgatggcaag cgcgcgcacg ccaagcgcct cgaccaccgc gccggaggtc tcgccgcccg
      721 cgaccacgaa tttgcgcacg cctttcgccg cgagcgcgcg cgcgatgtcg cgaaacgcct
      781 gctcgatcgc ctcgccgcgg ccctgcgcga tctccgcggc aggcgcgctt gcgtagagga
      841 gcggcgtgga gtccaggttg cgcgccgccc agtccacggc agctgcggca tccaatttgt
      901 gcggatcgag cctgagcgcg ggcgcggtcc aggccgcgat ctgtccaagc gtcgcctgcg
      961 agcagcttcc cgcgagcacg acggccggcc cgccgagccg cggcagggcc tgcgcggccg
     1021 ggcctcgctt tccgagcgcg cgcgcgatgc ccatggcgag ccccgcgccg cccgtggtga
     1081 gcttcatgtc gcggcacgcc tcgccggccg cgacgagatg ctcctcgcga gtggcgtcga
     1141 ggatcgcgta gcgcgccgtt ttctggagct cttcgaactt cttccggatg gcgcgtggtc
     1201 ctgcgtccat gacttcgaaa ggaaccaggc cgaccccgtg tggagtctgc gccgcgagca
     1261 cgcgcaccag gttcgagtcg cgcatgggcg tgagcggatg gtccttcatg cccgattcgg
     1321 cgaggggcac gtcgccgacg aagagattgc ctttgtagac cgtgcggcca ttgcgcggat
     1381 aggcgggcgt ggcgacgctg aagcgctcgg cgagcgcgtc gagcagcgca tcggcgaccg
     1441 gaccgatgtt gccgcgcgcg gtcgagtcga aggtcgagca gtacttgaag taaatgcgct
     1501 cggccttatg ggctcgtagc cagtccagag aagcgagaga ccaggcgacc gcctcggcgg
     1561 ccgggttggt gcgcgacttg agggcgacga cgacgcaatc ggccggcgga acggcgagcg
     1621 agcggtccgg cacgccgttc acctggatca ccgacaagcc ttcgcgcacg agcgtcacgc
     1681 cgaggtcggt ggcgcccgtc aggtcgtccg cgatgcaggc caggcgcatt gtcgattaga
     1741 ttgttgacaa tcctgtcgat gatactgaga atcggccgcc gaggagatac ttgtaccgca
     1801 ttgtcgacta acgaggagga gtcatgagat tcgtgaagtt cctgatcgcc actgcggcgc
     1861 tcgtgctgtg cgtgcaagcc caggcccagg taaagctcac gctgtggagc cattgggcgg
     1921 cggagaagat caagcgcgac tacgtcgagg acgccatcaa gcgcttcgag gcggcgaacc
     1981 cgggcgtgaa agtggaggcg cagtggtatg aaaagaccgc gctctacgcc gcgctgaaga
     2041 cggcgctgcg cgccggcacc gcgcccgaca tcttctacgc agagcccgac caggtggagt
     2101 acatggaaaa cgccctgctg ctcgatcttt ctgcgctcaa ctgggcggcg gtcgagccgt
     2161 gggcgaagga gatctggtcc tacaagggca agccgtacgg cctgccgctc gaggcctgga
     2221 cggtcgaggt gtactacaac aagaaaacct tgtccgacct cggcgtcgag gtgcccggca
     2281 accttcaact cgacgccgct accctcggcg atgtggcgaa aaaggcgcgc gcgaagaaca
     2341 tgacgccgat gtcgctcggc gtcggcgacc gcccgttccc cggtgcccac ctcacgcatg
     2401 aggcgctgct gaagaagctg ggcgtgcagg agtacgacaa cctgctcaaa ggcaagcttt
     2461 cctggagcga cccgcgggtg gtggaaacgc tgcgcgtggt gcgcggctgg gtcgaggcgg
     2521 gactgctgcc aacgaccttc accacgctca agctcggcga ggcgcatacc tacttccata
     2581 ccaaccccgg cgccgtcatg ttcctgaacg gaagctggta cacctcgcgt gcgttcaacc
     2641 cgccggacaa gggcggccag ccgcccaatt tcccgctcgg cgtgatgaag ttcccgaccg
     2701 tgccgggcgc ggcgtgcaac gagtgccgga ccatagcggt cggcggaagc tacgtcgcga
     2761 acgccgccac caggaacccg aagcaggcga tcgcgttcct gaactccttc gctacgcccg
     2821 agatggcgaa ccgctggctc gagaacgtgc tcgtgcagac ggggatcaag gccgactact
     2881 cgaagatcgc ggggccgcac gccggctatt tcaaggagct cggcgccacc agccagggcg
     2941 cgaagtacta cttcggcctg ccgatccagg tgatgcaggg caagcagaag gaggtcttca
     3001 cgcaggtgtt caacaacgcg tttccggccg gcactatttc cgtggacgaa gccgtcaagc
     3061 aaatgaacgc cgcgtacggc aagcattgac agcaagagcc agtcaggacc ggcagccgtg
     3121 gggcgccatc gtcgccttct tgctgccggc atttaccgtc tacgtcgcgc tcaccgccta
     3181 cccggtgctg cgcacgttct ggaacagctt ccacaaggtc cttccgcgca gcgaggaata
     3241 catcggcgcg gccaattacg cggccctggt ccaggacgag ctcttctggc gcgcagtgcg
     3301 caataccttc acctgggcgt gcatctcgcc cctggtggag gtgacgatcg cgctgctgct
     3361 cgcgctcgcc ctttacgccc gcgtaccggg catgcgtttc tttcgcattg cctggttcac
     3421 gccggtgctc atgtcctacg tcgtcgtcgg gatcatctgg gtctggatct acaactacga
     3481 ctggggcgcc gcgaacgcgc tgcttcgcct cctcgggttg ggagcgttcg tacaagcctg
     3541 gatcggcgac ccgaaactcg cgctgccctc gctcatcttc gtcaccacct ggatgtggac
     3601 cggcttcaac atggtggtcc ttctcgccgc gctgcactcg cttccctcgg aagtaatcga
     3661 ggcggccgag ctcgacaact gcggatgggg ggcgaagctc tggtacgtga tcgtgccgct
     3721 catcaagccc acgatcctca acctgctcgt gctttccttc atcggcaaga tgaagatctt
     3781 cgacctggtg tggatcacga cgaagggcgg accgctctgg tccaccgaaa ccgtgtccac
     3841 ctacctctac aagcgcgcct tcgagtggag cacgttcgat ctgggctatc cctccaccat
     3901 cgccgcagtg tggttcgtga tcgtgctcgc cgcggtgctc ggacttacct ggctgctgcg
     3961 cacgcgcggc cggctcgagt attgaatgaa aagaattttc ttgttgatca ttctggctct
     4021 gtacaccgcg tttgccgccg gcccgttcct ttgggtcgcg acgatgtcgg tgcgcactac
     4081 gagcgagatc gcgctcgacc actacgcctg gccggaacaa tggcactggg agaagttccc
     4141 gcaggcctgg ttcaactcca actacagcgt ctacttcgag aacagcctgc tcgtggtgct
     4201 cgcggcggtc gcggtgctga ccgtcgtcgg cgccatggcg gcgcactgcc tcgcacgcta
     4261 ccgctggcgc acgaaccggc tcttctactt catcctcttt tcggcaatca tcttcccgcc
     4321 gcagatcacg atcattgccc tcttccagat cctggtgcag tacgggctgt acaacagcct
     4381 gctcggcctc accgccgtgt acatcgccgt gcagctgccg ctcaccgtct acatcctcga
     4441 gagcttcttt gcgcgcattc cgtcggatct cttcgatgcg gcgcgcatcg acggctactc
     4501 ggagtggcag atcttctgga aggtgagcct gccgatcgcg ctgccggcca tcgccaccac
     4561 ggtgatcctg aacacgatcc tgctctggaa cgagtttctg tacgcggtcg tcctcatcac
     4621 cgacgaggcg aagcgcacgt tgccgctcgg catccagcgc ttcatgggcg accagctcga
     4681 ggacgtcggc atgatcgcga cagggttgat gatcgcgatc atcccagtcg tgctgatcta
     4741 cgcgttcttc tccgagaagc tgattcaggg catgaccgcg ggcgccgtca agtaatggcc
     4801 acggtgacgc tgcacgcgtt gtcgaagcgc tacccgggat cggcgcatct tgccgtcgac
     4861 gccgtggacc tcgacgttgc gcacggcgag ttcatggtgc tcctcggccc ttccggatgc
     4921 ggcaagtcga cgacgctgcg catggtggcc gggctcgaat cgatc
//
LOCUS       DASUNT010000492         2192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1207546, whole genome shotgun sequence.
ACCESSION   DASUNT010000492 DASUNT010000000
VERSION     DASUNT010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207546"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VFX94_09935"
     CDS             complement(<1..563)
                     /locus_tag="VFX94_09935"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5768541.1"
                     /translation="MSSSRLPPGLRLYLEPAPLAALFLGISSGFAFAMIGATLSTRLA
                     QHGITKSAVTAFALTFLAYNFKFLWAPIVDHVRLPLIGRFGQRRSWLWFVGVLVMGAV
                     AFLGLADPQEALYTVAVAAIVVGVAGATFDIIIDAYRIELLEPRQLGAGSGMSQYGWR
                     IGAAAAGALALVLAHRLGWAAAYIACAA"
     gene            complement(553..1326)
                     /locus_tag="VFX94_09940"
     CDS             complement(553..1326)
                     /locus_tag="VFX94_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX5768542.1"
                     /translation="MRVISLNVNGIRSAASKGLFRWLAGQRADVVCLQEMKCQEIDLD
                     AKLHGLKAYQSCHAFAKNKGYSGVSLYSRKTPDEMRRGFASREFDAEGRYVEARFGKL
                     WVVSMYMPSGSAGAHRQASKFRFLKAFLVHLKKIRSRGDEMILCGDWNIAHKEIDLRN
                     WRSNQKNSGFLPQERAWLSRVFDELGFVDVFRKLNDKPDQYTWWSNRGQAWKKNVGWR
                     IDYQIATPGIASRARRESIYKAKRFSDHAPLIIDYEHEL"
     gene            complement(1323..2012)
                     /locus_tag="VFX94_09945"
     CDS             complement(1323..2012)
                     /locus_tag="VFX94_09945"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX5768543.1"
                     /translation="MHFADQAFIAETTAKMLLEVKAVLFFSDKPFIFTSGWASPVYID
                     CRKLIFYPRIRTQLVDFAAGMLARNVGFEQFDVVAGGETAGIPYAAWIAERLGLPMQY
                     VRKKPKGFGRHAQIEGDIREGARTLLVEDLTTDGRSKINFCQALRAAGAIVEHVFVNF
                     YYDIFPESKKILADLNVRLHYLATWWDVLEVVKKGGYLPPKQVAEVESFLHAPAQWSA
                     AHGGVSSFPAS"
BASE COUNT          402 a          692 c          728 g          370 t
ORIGIN      
        1 gccgcgcagg cgatgtaggc cgcagcccag cccaggcggt gggcgagcac cagcgccagc
       61 gcgcctgcgg cggcggcgcc gatgcgccag ccatattgcg acatgccgga acccgcaccg
      121 agctggcgcg gctcgaggag ctcgatgcgg taggcgtcga tgatgatgtc gaaggtggcg
      181 cccgccacgc cgaccacgat ggcggcgacc gccaccgtat agagcgcctc ctgcgggtcg
      241 gcgaggccga ggaacgcgac cgcgcccatc accagcacgc cgacgaacca gagccagctt
      301 cgccgctggc cgaaccggcc gatgagcggc aggcgcacgt ggtcgacgat cggcgcccac
      361 aggaacttga agttgtaggc gaggaaggta agcgcgaagg cggtcaccgc gctcttggtg
      421 atgccgtgct gcgcaaggcg cgtgctgagc gtcgcgccaa tcatggcgaa ggcgaacccg
      481 gaggagatgc ccaggaaaag cgcggcgagc ggcgccggtt ccaagtatag gcgcaggccc
      541 ggcggcagcc tgctagagct catgctcgta atcgatgatg agcggtgcgt ggtcggagaa
      601 gcgctttgcc ttgtagatgg attccctgcg cgcgcgcgac gcaatgccgg gcgtggcgat
      661 ctggtagtcg atgcgccagc cgacgttctt cttccacgcc tggccgcggt tcgaccacca
      721 ggtgtactgg tcgggcttgt cgttcagctt gcgaaatacg tcgacgaagc cgagctcgtc
      781 gaacacgcgg ctgagccagg cacgctcctg gggtaggaag ccggaattct tctggttgga
      841 gcgccagttc ctgaggtcga tctccttgtg cgcgatgttc cagtcgccgc agaggatcat
      901 ctcgtcgccg cggctgcgga tcttcttcag gtgaaccaga aacgccttca ggaagcggaa
      961 cttcgaagcc tgccggtgcg ccccggcaga gccggagggc atgtacatgg ataccaccca
     1021 caacttgccg aagcgcgctt ccacgtagcg gccttcggcg tcgaactcgc ggctcgcgaa
     1081 gccgcgacgc atctcgtccg gcgtcttgcg gctatagagc gagacgccgc tgtagccctt
     1141 gttcttcgcg aatgcgtggc agctctggta ggccttgagc ccgtgcagct tcgcgtcaag
     1201 gtcgatctcc tggcacttca tttcctgcag gcacacgacg tcggcgcgct gcccggccag
     1261 ccaccggaac agtcccttgc ttgcggcgga gcggatgccg ttgacgttga gactgattac
     1321 tctcaagaag cgggaaacga agaaaccccg ccgtgagccg cggaccactg cgcgggtgcg
     1381 tgaaggaagc tttccacctc ggccacctgc ttcggcggga ggtaaccgcc cttcttcacc
     1441 acctcgagaa cgtcccacca ggtggccagg tagtggagcc tgacgttcag gtcggcgagg
     1501 atctttttcg actcggggaa gatgtcgtag tagaagttga cgaacacgtg ctcgacgatc
     1561 gcgccggctg cgcgcagcgc ctggcagaag ttgatcttcg agcgcccgtc ggtcgtgagg
     1621 tcctcgacca gcagcgtgcg tgcgccttcg cggatgtcgc cttcgatctg cgcgtggcgc
     1681 ccgaacccct tgggcttctt gcgcacgtac tgcatcggca gcccgagtcg ctcggcgatc
     1741 cacgccgcat agggaattcc tgccgtctcg ccgcccgcca cgacgtcgaa ctgctcgaag
     1801 ccgacgttgc gcgcaagcat gccggcggcg aagtcgacca gttgcgtgcg gatgcgtgga
     1861 tagaaaatca gcttgcggca gtcgatatac accgggcttg cccagccgga ggtaaaaatg
     1921 aacggcttgt ccgagaaaaa aagcacagcc ttcacctcga gcagcatctt ggccgtcgtc
     1981 tcggcaatga acgcctggtc ggcgaagtgc atgggcggcg cggattctaa caggtagaat
     2041 gccccgcatt cgcgctctgg gagggttgcg atgactataa gaactgcaat tttcgccgcg
     2101 gctgcgctga cgctcgcgcc gctcgccgcg cacacgcagc agcagagcga gcaactcacc
     2161 taccgctgca ccggcaagga tggcaagaag ta
//
LOCUS       DASUNT010000493         3319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1295926, whole genome shotgun sequence.
ACCESSION   DASUNT010000493 DASUNT010000000
VERSION     DASUNT010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295926"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFX94_09950"
     CDS             <1..363
                     /locus_tag="VFX94_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase"
                     /protein_id="HEX5768544.1"
                     /translation="ESADLYAFGPQHWARLVGLLGKVDRVMLDPPREGALEVARTLSG
                     ADAVPERLVYVSCNPATLARDCAVLVHEGNWCLRAAGVVNMFPHTTHVESIAVLEPAP
                     GRAVEARRASTSDARLAS"
     gene            468..893
                     /gene="ndk"
                     /locus_tag="VFX94_09955"
     CDS             468..893
                     /gene="ndk"
                     /locus_tag="VFX94_09955"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX5768545.1"
                     /translation="MAIERTLSIIKPDAVKKNVIGQILARFEAAGLRIVAARMMHLSR
                     AEAEGFYAVHRGRPFFGALVEFMTSGPVLVQVLEGDNAIARNRELMGATDPKKAAKGT
                     IRADFAGSIDANAVHGSDAPETARKEIAYFFPLSEVYSR"
     gene            890..1951
                     /gene="rlmN"
                     /locus_tag="VFX94_09960"
     CDS             890..1951
                     /gene="rlmN"
                     /locus_tag="VFX94_09960"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX5768546.1"
                     /translation="MTNLLGLDAAGLERFLHDEKAFRARQVLRWVHQRGEPDFDRMTD
                     IARELRAKLAAAACVEAPRIVGDTVAADGTRKWLLKVDGANAVEAVFIPEAKRGTLCV
                     SSQAGCVLDCAFCSTGKQGFNRNLSTAEVVGQLWLANQLLEGERPVTNVVLMGMGEPL
                     LNLDNLIPALRLMLDDNAYGLSRRRVTVSTAGVVPGMDRLREECPVALAVSLHAPNDA
                     LRDRLVPVNRKYPLAQLIAACNRYLEKAPRDFITFEYVMLGGVNDAEGHARELVGLAA
                     RVRSKFNLIPFNAFANSGFKRSSPERIRRFAEVLQRAGLTVTTRKTRGDDIAAACGQL
                     AGDVADRTRRTLLLKEARA"
     gene            1948..2724
                     /gene="pilW"
                     /locus_tag="VFX94_09965"
     CDS             1948..2724
                     /gene="pilW"
                     /locus_tag="VFX94_09965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEX5768547.1"
                     /translation="MSVRVAILVAMLAGCAGQTPHPVDDSAATLKGEAADKRNRARLH
                     TELASLYYTNGNMAIALEELRAATTADAGYGPAHGMYGVVYMRLNETAKAAESFERAL
                     RLSPSDPDINHNYGWFLCQTGREDASMKHFQTAVRNPLYPAPWRSYAAAGVCSLKMNQ
                     VKEAEAFFERALKLEPDEPSSLLQLGQIRYKQGNIGEARKLVTRHNKVVTPSAESLWL
                     AVRIERRVGDRLQEQAYANQLRRRYPASPEYEAMQRGLYD"
     gene            2717..>3319
                     /locus_tag="VFX94_09970"
     CDS             2717..>3319
                     /locus_tag="VFX94_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5768548.1"
                     /translation="MTEVTGVGQELSAAREARGLAISDVAHRLKFAPRQVEALEQEQF
                     DALPGATFTRGMVRNYARLLQLDPEPLVQRVAVRCQVPDSRQLAVRYQQPVPFSDSTR
                     RSSFTYLVVSLGVLGLVAGVAYQWQQERAAAARKTATVVAKAQPAQRSIQPAPLAEAT
                     AAPAPVPAAAPSAPAEPVVQEKVIENVPAEPKALVPAAPAA"
BASE COUNT          546 a         1134 c         1122 g          517 t
ORIGIN      
        1 gaaagcgccg acctgtatgc gttcgggccg cagcattggg cacgcctggt cggcttgctg
       61 gggaaggtgg atcgcgtgat gctcgatccg ccaagagagg gcgcactcga agtggcccgt
      121 acgctatccg gcgccgatgc ggtgcccgag cggctcgtct acgtctcgtg caatccggcc
      181 acgctggccc gcgactgcgc ggtgctggtc cacgaaggga actggtgcct gcgggcggcc
      241 ggtgtggtca acatgttccc gcacaccacc cacgtcgaat cgatcgccgt cctcgaacct
      301 gcgcccggcc gcgcggtcga agcgcgccgc gccagcactt ccgatgctag actggcgagc
      361 taagttgtgc tcgcaaagcc ttcatttctc gggacttttt gtcggaccgg gcggtgggct
      421 gcgtatcccg gcacatcgcc ctagtccaac tcgcagaaac gatccacatg gccatcgaac
      481 ggaccctttc catcatcaag cccgacgccg tcaagaagaa cgtgatcggg cagatcctcg
      541 cgcgcttcga agcggcgggc ttgcgcatcg tcgcggcgcg catgatgcac ctttcgcgcg
      601 ccgaggctga aggcttctac gccgttcatc gcgggcggcc tttctttggc gcgctcgtgg
      661 agttcatgac ctcggggccg gtgctggtac aggtgctcga aggggacaat gctatcgcgc
      721 gcaaccgtga gctcatgggc gcgaccgacc cgaagaaggc cgccaaaggc accatccgcg
      781 cggatttcgc cggctcgatc gatgccaacg cggtgcacgg ctcggatgcg cctgagacgg
      841 cgcgcaagga gatcgcctac ttttttccgc tgtccgaggt ctactcgcga tgacgaacct
      901 gctcggcctc gacgcggccg gcctggagcg cttcctccac gacgaaaagg ccttccgggc
      961 gcgccaggtc ctgcgctggg tgcaccagcg cggggaaccc gacttcgacc gtatgaccga
     1021 catcgcgcgc gagctgcgcg cgaagctggc agccgctgcg tgcgtcgaag cgccgcgcat
     1081 cgtgggcgac accgttgccg cggacggcac gcgcaaatgg ctgctcaaag tggacggtgc
     1141 gaacgccgtc gaggcggtgt tcattcccga ggcgaagcgc ggcacgctgt gcgtttcgag
     1201 ccaggcgggc tgcgtgctcg actgcgcgtt ctgttccacc ggcaagcagg gtttcaaccg
     1261 caacctgagc acggccgagg tcgtgggcca gctatggctc gccaaccagc tgctggaagg
     1321 cgagcgcccc gtcaccaatg tggtcctgat gggcatggga gagccgctgc tcaatctcga
     1381 caacctgatt ccggcgctgc gtctgatgct cgatgacaac gcctatggcc tttcgcggcg
     1441 gcgtgtcacc gtgtcgactg cgggagtggt gcccggcatg gaccggctgc gcgaggagtg
     1501 cccggtggcg ctcgcggtat ccctgcacgc gcccaacgac gcgctgcgcg accgccttgt
     1561 gcccgtcaac cgcaagtacc cgctcgcgca gctcatcgcg gcgtgcaacc gctacctgga
     1621 aaaggcgccg cgcgacttca tcaccttcga atacgtgatg ctcggcggcg tgaacgatgc
     1681 ggaaggccac gcgcgcgagc tggtcgggct cgcggcccgg gtgcgcagca agttcaacct
     1741 gatcccgttc aacgcgtttg ccaattcggg cttcaagcgc tcatcgccgg agcgcatccg
     1801 gcgctttgcc gaggtgctgc agcgggcggg gctcaccgtg acgacccgca agacgcgcgg
     1861 cgacgacatc gccgccgcgt gcggccagct tgcgggcgac gttgccgacc gtacccggcg
     1921 cacactcctc ctcaaggaag cacgagcatg agcgtccgcg tagcgatcct cgtcgcaatg
     1981 cttgccggct gtgcgggaca gactccccat ccggtcgacg acagcgcggc gacgttgaaa
     2041 ggcgaggccg ccgacaagcg caaccgggca cggctgcata ccgagcttgc ctccctctat
     2101 tacaccaacg gcaacatggc gatcgcgctg gaggagctgc gcgccgcgac gacggcggat
     2161 gccggctatg gtccggcgca cggcatgtac ggggtggtgt acatgcggct caacgaaacg
     2221 gcgaaggccg cagagagctt cgagcgcgcg ctgcgccttt cgccgagcga ccccgacatc
     2281 aaccacaact acggctggtt cctgtgccag acgggccgcg aggacgcatc gatgaagcat
     2341 ttccagactg cggtgcgcaa tccgctctac ccggcgccgt ggcgctcgta cgcggcagcg
     2401 ggtgtgtgct cgctgaagat gaaccaggtg aaagaagctg aggcgttctt cgagcgcgcg
     2461 ctgaagctcg agcccgacga gccttcctcg ctgctgcagc tcggccagat ccgctacaag
     2521 cagggcaaca tcggcgaggc gcgcaagctg gtcacgcgcc acaacaaggt agtgacgccg
     2581 agcgccgaat cgctctggct tgcggtgcgc atcgagcgcc gcgtcggcga ccggctgcag
     2641 gagcaggcct acgccaacca gctgcgccgg cgctatccgg cgtcgccgga atacgaggcc
     2701 atgcagcgcg gactgtatga ctgaggtcac gggggtcgga caggagctca gcgcggcgcg
     2761 cgaggcgcgc ggcctggcga tatccgacgt cgcgcatcga ctcaagttcg cgccgcgcca
     2821 ggtcgaggcg ctcgagcagg agcagttcga cgcgctgcca ggcgccacct tcacgcgcgg
     2881 catggtgcgc aactacgcgc ggctcctcca actcgacccg gagccgctag tgcagcgcgt
     2941 cgccgtgcgc tgccaggtcc cggattcccg gcagcttgcc gtgcgctacc agcagccggt
     3001 cccgttttcc gacagcaccc ggcgctcgag tttcacctac ctcgtcgttt cgctcggtgt
     3061 gctcgggctt gtcgcgggcg tggcgtacca gtggcaacag gagcgcgcag ccgcagcgag
     3121 gaagacggcg acggttgtgg ccaaggccca gccggcccag cgctccatcc agcccgcccc
     3181 gctcgccgag gccacggcgg cgccggcacc cgtcccggca gcggcgccct ctgcgcccgc
     3241 tgaacccgtg gtccaggaaa aagtgatcga gaacgtcccg gccgagccga aggcgctcgt
     3301 gcctgcggcg ccggcggca
//
LOCUS       DASUNT010000494         2640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_593057, whole genome shotgun sequence.
ACCESSION   DASUNT010000494 DASUNT010000000
VERSION     DASUNT010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593057"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VFX94_09975"
     CDS             <1..715
                     /locus_tag="VFX94_09975"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5768549.1"
                     /translation="TPDDVVERMLVMAQVTPNDFVWDLGAGDGKIAIMAAKKFGARAI
                     GIEYNADMVKHANENAQKAGVAGTGQGKATIRHGDIFATDFTQATVVTLYLLPALNMK
                     LRPQLLSMRPGTRIVSHSFSMEDWEADEISTLDGRRAYFWIVPANVMGGWNLEAGGQR
                     QEMTLEQTFQKVSGTLALGPIHAGLRDVKLRGTSISFAYVDQNKVRRDFTGRIDGAKM
                     EGTFRDEQGAEGKWTATKK"
     gene            complement(740..985)
                     /locus_tag="VFX94_09980"
     CDS             complement(740..985)
                     /locus_tag="VFX94_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX5768550.1"
                     /translation="MAEPLIAQRSPYAVELAPGDYWWCRCGQSRRQPFCDGSHKSTEF
                     TPVKFSMSEAQKVWLCGCKRSGNKPFCDGSHKKLPES"
     gene            complement(970..2394)
                     /gene="dnaB"
                     /locus_tag="VFX94_09985"
     CDS             complement(970..2394)
                     /gene="dnaB"
                     /locus_tag="VFX94_09985"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX5768551.1"
                     /translation="MPVAQPVARSDAADRELVALRLPPHSVEAEQSLLGALLLDNQAF
                     DRVADIVSSQDFYRDDHRRIWRHVARLIEATRPADVVTVSESIEASEDKDKTGGASYL
                     AALAQNTPSALNIRRYAELVRERAVQRRLAQVATEIAESALAPTGKDVGQLLDEAESR
                     IFQIAESGARKDQGLVGISPVLARVFERIDQLHSQENPSDVTGVPSGFVDVDRKTAGL
                     QPGDLIIVAGRPSMGKTAFALNIAEHVALHPSVSMPVAIFSMEMSASQLAMRMLSSMA
                     RVDQSKLRTGRLDNEEWANLTDAIGKLNEARIHVDETAALNALELRARARRMKREYGK
                     LGLVVVDYLQLMSATSQGENRATEISEISRSLKALAKELDVPVIALSQLSRAVEQRND
                     RRPLMSDLRESGAIEQDADVILFIYREEVYAPEKEEARGRAEVIIGKQRNGPIGTIGL
                     TFMGQFTRFANALAFDGGGQWPNR"
BASE COUNT          437 a          878 c          867 g          458 t
ORIGIN      
        1 gacccccgac gacgtggtcg agcgcatgct cgtgatggcg caggtcacgc ccaacgactt
       61 cgtctgggac ctgggggcgg gcgacggcaa gatcgcgatc atggcggcga agaagttcgg
      121 tgcgcgcgcc atcggcatcg agtacaacgc ggacatggtg aagcacgcga acgagaacgc
      181 gcagaaggcg ggcgtggcag ggaccggaca gggcaaggcg acgatccgcc acggcgacat
      241 cttcgccacc gacttcaccc aggccaccgt ggtcacgctg tacctgctgc ccgcgctcaa
      301 catgaagctc aggccccagc tgctgtcgat gcggcccggc acgcgtatcg tgtcgcactc
      361 cttcagcatg gaggactggg aagccgacga aatctccacc ctggacggcc gccgcgccta
      421 cttttggatc gtgcccgcca acgtgatggg tggctggaac ctcgaggccg gcgggcagcg
      481 ccaggagatg acgctcgagc agaccttcca gaaggtaagc gggacgctcg ccctgggccc
      541 gatccacgcc gggctgcgcg acgtcaagct gcgcggcacg tcgatcagct tcgcctacgt
      601 cgaccagaac aaggtgcgcc gcgacttcac cggccgcatc gacggcgcca agatggaagg
      661 caccttccgc gacgagcaag gcgcggaagg aaagtggacc gccaccaaga agtagccgtt
      721 cggggacgga gcccgattct taggactcag gcagcttctt gtgtgatccg tcgcagaaag
      781 gcttgttgcc gctgcgcttg cagccgcaca gccagacctt ctgcgcctcg ctcatgctga
      841 acttcaccgg cgtgaactcg gtgctcttgt gcgagccgtc gcagaacggc tggcgcctcg
      901 attgcccgca gcggcaccac cagtagtctc cgggcgcgag ctcgacggcg tagggggatc
      961 tttgcgcgat cagcggttcg gccattgtcc gcctccgtca aaagcgaggg cgttcgcgaa
     1021 gcgggtgaac tgacccatga acgtcagccc aatggtacct atcggcccgt ttctttgctt
     1081 gccgatgatc acctcggccc tgcccctcgc ctcctccttc tccggtgcat acacctcctc
     1141 gcggtagatg aagaggatga cgtctgcgtc ctgctcgatc gcgcccgact cgcgcaggtc
     1201 ggacatgagc ggccggcgat cgtttcgctg ctcgaccgcg cgcgagagct gagacagcgc
     1261 gatcaccggc acgtcgagct ccttcgcgag cgccttgagc gaacgcgaga tctcggagat
     1321 ctcggtggcg cggttttcgc cttgcgaggt cgccgacatg agctgcaggt aatcgaccac
     1381 cacgaggccg agcttgccgt attcgcgctt catgcgccgc gcccgcgcgc gcagctcgag
     1441 cgcattcagc gccgcggtct cgtccacgtg gatgcgcgcc tcgttcagct tgccaatggc
     1501 gtcggtgagg ttggcccatt cctcgttgtc gagccttccg gtgcgcagct tggactggtc
     1561 cacccgcgcc atcgacgaaa gcatccgcat cgcgagctgg ctcgcgctca tttccatgct
     1621 gaagatcgcg accggcatgc tgacgctcgg atgaagcgcg acgtgctctg cgatattgag
     1681 cgcgaacgcg gtctttccca tcgacgggcg ccccgcgacg atgatcaggt cgccgggctg
     1741 cagcccggcg gtcttgcggt cgacgtcgac gaagcccgac ggcacgccgg tgacgtcgga
     1801 gggattttcc tggctgtgga gctggtcgat gcgctcgaac accctcgcga gcaccgggct
     1861 gatgcccacc aggccctggt ccttgcgcgc gcccgactcg gcgatctgga agatcctgga
     1921 ctccgcctca tcgaggagct gccccacgtc cttgccggtc ggggcgagcg cgctctcggc
     1981 gatctcggtg gcgacctgcg cgaggcgccg ctgcacggcg cgctcgcgca cgagctccgc
     2041 gtagcgccgg atgttgagcg cgctcggggt gttctgcgcg agcgccgcga ggtaggacgc
     2101 gccgccggtc ttgtccttgt cctcgctcgc ctcgatcgac tccgacaccg tcaccacgtc
     2161 ggccggccgg gtcgcctcga tcaggcgcgc gacgtgccgc cagatgcggc ggtggtcgtc
     2221 gcggtagaaa tcttgcgacg aaacgatgtc cgcgacgcgg tcgaaggcct ggttgtcgag
     2281 gagcagcgct ccaagaagcg actgctcggc ttccaccgag tgcggcggaa gcctgagcgc
     2341 gacgagctcg cgatcggcgg cgtccgatcg cgctaccggc tgtgcaacgg gcatcttgtc
     2401 tgaccctccc cggcgcgcat cataggagcg cggcggcgat ttcgatttga taagtcgaaa
     2461 cgtgcgcgct agccacgctc tcgcgccgcc gtgctgctga aaatttgacc acccggggct
     2521 agccggccgc gacggctgtg gacagcctgt gcgcaggctg tcggtagttt gtggatgacg
     2581 ctgtacaact ccaaattaaa gttcttgaca cgccgcatca ctctattact gtataaacgt
//
LOCUS       DASUNT010000495         3265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_156480, whole genome shotgun sequence.
ACCESSION   DASUNT010000495 DASUNT010000000
VERSION     DASUNT010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156480"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VFX94_09990"
     CDS             <1..587
                     /locus_tag="VFX94_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX5768552.1"
                     /translation="DGRSYALRPKVLGLGHAYLSSTPLVIAAQPFLDRVSEAVNESCS
                     LATLDGEDILYLARSVTSRIISVTLNVGSRLPAHCTSIGYVLLANLPDKELDAFLAKA
                     ELKAYTERTLTSPEKLREQLEQVREADYAIADRLMELAVRSIAVPVRNAAGTVVAGMN
                     VIVEAGRVSLRDMRSLYLPHLQSAAAELGAQLAP"
     gene            584..1405
                     /locus_tag="VFX94_09995"
     CDS             584..1405
                     /locus_tag="VFX94_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX5768553.1"
                     /translation="MSQPCIITVAITGSLPGKKDNPAVPISVEEQIESTHEAFEAGAS
                     LVHLHVRDDEGRPTSDPKRFARVLEGIRRHCPGMITQLSTGGRSGTGRERGGMLALKP
                     DMASLATGSVNFPTRVYENSPELIDWLAAEMIKYGVKPEVEAFDLSMIFQAAAMQKAG
                     KIKGPLHVQFVMGVKNAMPVDRAAFEFFVQTLKRLDPDATWTGAGIGKDQVTVSRWAA
                     ELGGHCRTGLEDNVRMSREKLAPSNAALVRQTVALCAEYGRPPATAAEARSMLSL"
     gene            1402..2256
                     /locus_tag="VFX94_10000"
     CDS             1402..2256
                     /locus_tag="VFX94_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX5768554.1"
                     /translation="MTVQERLPEPSNPVGIDGLEFIEFATSQPQALGAVLQKMGFEPV
                     ARHRSREVMLYRQGTMNLIVNAHDAKEPAPEIKAIALRVRDAGQAWAHALALGAWEMP
                     TRASAMELNIPGIHGVGDSLIYFVDRYRDFSIYDVDFVYEKNVERNPPALAGLHYFGV
                     VQSIYGERTRDWVDFYRNLFGFSVLPEGKYFGVLPKGTLLESPCHKFYLQLIEPPPGS
                     EDIHWEEALVRVGLGASDVPAATRALQGRGVVFVDRGPVQPSEKGALTQVYLGGVTFE
                     LVVSHLQP"
     gene            2253..3122
                     /locus_tag="VFX94_10005"
     CDS             2253..3122
                     /locus_tag="VFX94_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX5768555.1"
                     /translation="MNLDHFGMDTITLAGPLEGKLRAMRAAGFTQVMLNANDLAGHPG
                     GPDAAIDTVRASGLRVTGFQVLRDFEGLSGHLHAYKVDIAKAMLSLCRALGSRVLLAC
                     SSTSSHATGEFDSLTRDLQKLAMLAVPYGIRIAYEALSWGRHVNEYPQAWQIVGDADR
                     ANRGLALDSFHRLATGTGREALEEIDPSKIFLVQLADFMWREIRSREERIDTARHFRV
                     FPGEGVHGAEVSALVRTLDRLGYRGDYSFEVFNDDYTQLPHAVVAERARRSVKWLTDQ
                     VSRRSLPLRGATR"
BASE COUNT          532 a         1136 c         1078 g          519 t
ORIGIN      
        1 acgacggccg cagctacgcc ctgcggccga aagtgctcgg cctcggccac gcctaccttt
       61 cctccacgcc gctcgtcatc gcggcacagc cttttctcga ccgggtgagc gaggcggtga
      121 acgagtcctg ctcgctcgcc acgctggacg gcgaggacat cctgtacctc gcccgctcgg
      181 tcacctcgcg catcatctcg gtcacgctga acgtcggcag ccggctgccc gcgcattgca
      241 cctcgatcgg gtacgtgctg cttgcgaatc tgccggacaa ggagctcgat gcctttctcg
      301 ccaaggccga gctcaaggcc tacaccgagc gcaccctcac ctcgccggag aagctgcgcg
      361 agcagctcga gcaggtccgc gaggcagatt acgccatcgc cgaccggctg atggagctcg
      421 ccgtgcgctc gatcgccgtg ccggtgcgca atgccgcggg cacggtggtt gcggggatga
      481 acgtcatcgt cgaggccgga cgggtgtcgc tgcgcgacat gcgcagcctg taccttcccc
      541 atcttcagtc cgccgccgcc gaactcggag cccagctcgc gccatgagcc agccgtgcat
      601 cattaccgta gcgatcaccg gctcgctccc gggaaagaag gacaacccgg ccgtgccgat
      661 ctcggtcgag gagcagatcg agtcgacgca cgaagcgttc gaagccggcg cgagcctcgt
      721 gcatctgcac gtgcgcgacg acgaaggcag gccgacttcc gacccgaaac gcttcgcgcg
      781 cgtgctggaa ggcatccgca ggcactgccc tggaatgatt actcagcttt ccaccggcgg
      841 gcgctccgga accgggcgcg agcgcggcgg gatgcttgcg ctcaagcccg acatggcgtc
      901 gctcgccacc ggctcggtga actttccgac gcgcgtctac gagaactcgc ccgagctcat
      961 cgactggctc gcagccgaga tgatcaagta cggcgtcaag ccggaagtcg aggcgttcga
     1021 tctgtcgatg atcttccagg cggccgcaat gcagaaagcc ggcaagatca agggcccgct
     1081 gcacgtccag ttcgtcatgg gcgtgaaaaa cgcgatgccc gtggaccggg ccgcattcga
     1141 gttcttcgtg caaacgctca agaggcttga tcctgatgcc acgtggaccg gcgccgggat
     1201 cggcaaggac caggttacgg tctcccgctg ggcggccgag cttggcggcc attgccgcac
     1261 cggcctcgag gacaacgtgc gcatgagccg cgagaagctc gcgccttcca acgccgcgct
     1321 cgtgcggcag acggtggcgc tgtgcgcgga atacggccgc ccgcccgcaa cggcggccga
     1381 ggcgcgtagc atgttgtcgc tatgaccgtg caggagcgcc tgcccgagcc gtcgaacccg
     1441 gtcggcatcg acgggctgga attcatcgag ttcgccacgt cgcagccgca ggcgctcggc
     1501 gctgttttgc agaagatggg cttcgagccg gtggcgcggc atcgctcgcg cgaggtgatg
     1561 ctttatcgcc agggcacgat gaacctgatc gtcaacgcgc acgacgccaa ggagccggcg
     1621 cccgaaatca aggcgatcgc gctcagggtg cgcgacgccg ggcaggcgtg ggcgcacgcg
     1681 ctcgcgctcg gcgcatggga gatgcccact cgcgcatcgg ccatggagct caacatcccg
     1741 ggcatccacg gcgtgggcga cagcctgatt tacttcgtgg accgctaccg ggacttctcg
     1801 atctacgacg tcgacttcgt ctacgagaag aatgtggagc gcaatccgcc ggcgctcgcc
     1861 gggctgcact acttcggcgt ggtgcaaagc atctacggcg agcgcacgcg cgactgggtc
     1921 gatttctacc gcaatctctt cggcttctcg gtgcttcccg agggcaagta tttcggcgtg
     1981 ctgccgaaag gcacgctgct tgaaagcccg tgccacaagt tctacctcca gttgatcgag
     2041 ccgccgcccg gctcggagga catccactgg gaggaggcgc tggtgcgcgt cggcctgggc
     2101 gcgtccgacg tgccggccgc gacgcgtgcg ctgcagggcc gcggcgtggt gttcgtcgac
     2161 cgcgggccgg tgcagccgag cgagaaaggc gcgctcaccc aggtctacct gggcggcgtg
     2221 accttcgagc tggtggtcag ccacctgcag ccatgaatct cgatcacttc gggatggata
     2281 ccatcacgct ggccgggccg ctcgagggca agctgcgcgc gatgcgcgcc gccgggttca
     2341 cgcaggtgat gctgaacgcc aacgacctcg ccgggcatcc cggcggcccg gacgccgcca
     2401 tagacacggt gcgcgcgagc gggctgcggg tcaccggctt ccaggtgctg cgcgacttcg
     2461 aaggactgtc gggtcacctg cacgcctaca aggtggacat cgccaaggcg atgctctcgc
     2521 tgtgccgcgc cctcggctcg cgcgtcctgc tcgcatgctc gtcgacctcc tcgcatgcca
     2581 ccggcgagtt cgattccttg acgagggacc tgcagaagct cgccatgctc gccgtgccgt
     2641 acggcatccg catcgcctat gaagccctct cctggggaag gcacgtcaac gagtacccgc
     2701 aagcctggca gatcgtgggc gatgccgacc gcgcgaaccg cggcctcgcg ctcgattcgt
     2761 tccacaggct cgccaccggg accggccgcg aggcgctgga ggagatcgac ccgtccaaga
     2821 tctttctcgt ccagctggcc gacttcatgt ggcgcgagat ccgctcgcgc gaggagcgca
     2881 tcgacaccgc gcgccatttc cgcgtgttcc cgggcgaagg cgtgcacggc gccgaagtct
     2941 ccgccctggt gcgcaccctc gaccggctgg gctaccgcgg cgattacagc ttcgaggtgt
     3001 tcaacgacga ctacacccag ctgccgcacg cggtggtggc cgagcgcgcc cgccgctcgg
     3061 taaaatggct gaccgaccag gtgtcgcggc gcagcctgcc gctgcgcgga gcgacccgtt
     3121 gaagcaaccg ctacatgcaa tcgctacgcc tctacgacga gctcaccgcc gcgcgcacgc
     3181 gcttcctgcg cgtcgacgag ctttgccgcc gcgccgctga gaaaggcgcc cttccctccg
     3241 ccgaagcgct ggcggccgac gcgcg
//
LOCUS       DASUNT010000496         3826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_856839, whole genome shotgun sequence.
ACCESSION   DASUNT010000496 DASUNT010000000
VERSION     DASUNT010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856839"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFX94_10010"
     CDS             <1..229
                     /locus_tag="VFX94_10010"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEX5768556.1"
                     /translation="ITTDPNPVVRAVHDRMPLMVPRSLYQAWLHGDETILRSAPEIEL
                     RAHPVSMALNQAANDSPEMIEPVELARGLFD"
     gene            complement(206..1246)
                     /gene="dinB"
                     /locus_tag="VFX94_10015"
     CDS             complement(206..1246)
                     /gene="dinB"
                     /locus_tag="VFX94_10015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX5768557.1"
                     /translation="MRRVVHLDMDAFYASVEQRDHPELRGKPVAVGGRPQARGVVAAA
                     SYEARAFGVRSAMPMARALRLCPELLIVSPDFERYRRVSSQVMAILRACTPLVEPLSL
                     DEAYLDVTENAWGEEFGSRVARRLKQQIVEETGLTASAGVAPNKFLAKIASGYRKPDG
                     LTVISPERVESFLQELPVEALWGVGPVTARKLRGLGIERLVQIRTADVQLLRDTVGSL
                     ADWLRQLSQGDDPRPVVPDRESKSSSTENTYAHDLVDADAIRAELDRMARENADWLAR
                     RRLAARTVTIKVRYSDFKTVTRSHTLSRPTSDADLIASWAVELAARTQAGTKAVRLLG
                     VRVQGLVEQAAG"
     gene            1266..1895
                     /gene="lexA"
                     /locus_tag="VFX94_10020"
     CDS             1266..1895
                     /gene="lexA"
                     /locus_tag="VFX94_10020"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX5768558.1"
                     /translation="MDGLTERQAQILRLVRELTEVSGFPPTRAEIAERMGFRSVNAAE
                     QHLRALEKKGAIEISSGASRGIRVRDGRPAARLGRLLELPLVGRVAAGSPILAEENLQ
                     GRYQVDPNLFTPRADYLLRVRGMSMRDAGILEGDLLAVHKTSEARSGQIVVARLADEV
                     TVKRYRRRGHTVELEAENPDYRPIEIDLRREPLTIEGTAVGVIRNGKAL"
     gene            1933..3741
                     /locus_tag="VFX94_10025"
     CDS             1933..3741
                     /locus_tag="VFX94_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5768559.1"
                     /translation="MNHTTTADGSAARLEVSTNAVEAEIRDRADPRQVLADRVAQLYR
                     QMPIAIGATFIAGAIATFELQGRWLKELVLIWWAIVVTFTAAASLLLYAYYRSPDKVA
                     SAPQWLRWLGIAALGNGASWGLAGGVFFRSLSDEQQVFLAFLFAGMASVGIPVYAASW
                     PIFALYAAGILGPFFYVLLTFGNRLFVEIALLVPLFYAINVVIAYRRTQVFHSGYRLR
                     HAYGRLTRDHRQLNQRLERQVVELEEARRQVEASGRKLALFAERAPIAVLELEPDGTV
                     VEVNHAAEILFGYAAAELVGGPVKKLVLPKFHEDFDHTWQQLVTSRESVAGLKIRNPR
                     RDGAEIVCEWTVTPLVNFQREVIAVIAQGRDITAQLEAERMKKEFTSTLSHELRTPLT
                     SIIGSLQLINGGVMGEVDKEVAELTMVAERNGQRLLDLINDILDIEKIESGKLTLAPE
                     LIALDELVRESMVLNKAFGERFQVRFEAHGRMSNTRVHADRKRLLQVMTNLLSNAAKF
                     SPEGGVVEISTADVGPSVRVSVHDRGSGIPEAFRQRIFGRFTQADSTATRQKGGTGLG
                     LAICRRLLELMHGRIGFDDRPGGGTTFWFELPRHSA"
BASE COUNT          634 a         1329 c         1241 g          622 t
ORIGIN      
        1 cattactacg gatccgaacc cggtagtacg cgccgttcac gatcgcatgc ccctcatggt
       61 gccaaggagc ctttaccagg cgtggctgca cggcgacgaa accatcctcc ggtcagcgcc
      121 cgagatcgag ctgcgcgctc atccggtcag catggcgcta aaccaggcgg cgaacgattc
      181 tccggagatg atcgagccgg tcgagctagc ccgcggcctg ttcgactaat ccctgcacgc
      241 gcacaccgag gagccgcacc gccttcgttc ccgcctgggt gcgcgcggcg agctccaccg
      301 cccacgaggc aatgaggtcc gcatccgacg tagggcgcga cagcgtatgg ctgcgcgtca
      361 cggtcttgaa atcggaatag cgcaccttga tcgtcacggt gcgcgctgcg agccgccgcc
      421 gcgcgagcca gtcggcgttc tcgcgtgcca tgcgatcgag ctcggcgcgg atggcatccg
      481 cgtcgacgag atcgtgcgca tacgtattct ccgtgctgga agacttcgac tcgcggtccg
      541 gtactaccgg ccgcggatcg tcgccttgcg agagctggcg cagccagtcg gcgaggctgc
      601 ccacggtgtc gcgcaggagc tgcacatccg cggtgcggat ctgcaccagc cgttctatcc
      661 cgagcccgcg cagcttgcgg gcggtcaccg gccctactcc ccacagcgct tccaccggca
      721 gctcctggag gaagctctcc acgcgctcag gcgagatcac ggtaagcccg tcgggcttgc
      781 ggtagccgga ggcgatcttg gccaggaact tgttcggcgc gacgcccgcc gaagcggtga
      841 gccccgtctc ctcgacgatt tgctgcttca gccgtcgggc gacgcggcta ccgaactcct
      901 cgccccaggc gttctcggtg acgtccagat aagcctcgtc gagcgaaagc ggctccacca
      961 gcggcgtgca agctcgcagg atggccatca cctggctcga cacgcgccgg tagcgctcga
     1021 agtccggaga cacgatcagg agctcagggc agagtctcag cgcgcgcgcc atcggcatcg
     1081 ccgagcgcac gccgaacgcg cgcgcctcat agctcgcggc cgccaccacg ccgcgcgcct
     1141 gcggccggcc cccgaccgcc accggcttcc cgcgcagttc cgggtggtcg cgttgctcga
     1201 ccgaggcgta gaacgcgtcc atgtccagat ggacaacccg ccgcatactg tatatattgc
     1261 cactaatgga cggacttaca gagcggcaag cgcagatcct acgcctcgtc cgggagctga
     1321 ccgaggtctc gggttttccg ccgacccgcg ccgagatcgc cgagcgcatg ggctttcgct
     1381 ctgtgaacgc tgccgagcaa cacctgcgcg cgctggagaa aaagggcgcg atcgaaatct
     1441 cttccggagc ctcgcgcggc atccgggtgc gcgatggccg ccctgccgcc cgcctcgggc
     1501 ggctgctcga gctgccattg gtcggccgcg tcgccgcggg cagcccgatc ctcgccgagg
     1561 agaacctgca gggccgctac caggtcgatc ccaatctgtt cacgccgcgc gccgactacc
     1621 tgttgcgcgt gcgcggcatg tcgatgcgcg acgccggaat cctcgagggc gacctgctcg
     1681 ccgtgcacaa gacgagcgaa gcgcgcagcg ggcagatcgt ggtcgcccgg ctcgccgacg
     1741 aggtcaccgt gaagcgctat cgccggcgcg gacatacggt ggagctcgaa gcggagaatc
     1801 cggattacag gccaatcgag atcgacctgc ggcgtgagcc gctcaccatt gaaggaaccg
     1861 ccgttggcgt aatacgcaac ggcaaggccc tctaagacat cttagaatgg cgctggcccc
     1921 aggcccgcgc cgatgaatca cactacaacc gcagacggca gcgccgcccg actggaggtc
     1981 tcgaccaatg cggtcgaggc cgagattcgc gatcgcgccg acccgcggca ggttctcgcc
     2041 gaccgcgtcg cccagctcta ccgccagatg ccgatcgcca tcggcgcgac cttcatcgcc
     2101 ggcgcgatcg ccaccttcga gctgcaaggc cggtggctga aggagcttgt gctgatatgg
     2161 tgggcgatcg tcgtcacctt cactgcagcg gcgagcctgc tgctctatgc gtattaccga
     2221 agcccggaca aggtcgcgag cgcgccgcag tggctgcgct ggctcgggat cgcggcgctc
     2281 ggcaacggcg cgagttgggg gctcgcgggc ggtgtgttct tccgctcgct gtcggacgag
     2341 cagcaggtgt ttcttgcgtt tctcttcgcc gggatggctt cggtgggcat cccggtgtac
     2401 gccgcgtcgt ggccgatctt cgctctctac gcggccggca ttctgggccc gtttttctac
     2461 gtgctgctca ccttcggcaa tcgcctgttc gtagagatcg cgctgctcgt gccgctcttc
     2521 tatgcgatca acgtcgtcat tgcttaccgg cgaacgcagg tgttccactc cggctaccgg
     2581 ttgcggcacg cctacggcag gctcacccgc gaccaccgcc agctcaacca gcgcctcgag
     2641 cggcaggtgg tcgagctcga ggaggcgcgg cgccaggtcg aggcgagcgg ccgcaagctc
     2701 gcgctcttcg ccgagcgcgc gccgattgcg gtgctcgagc tcgagcccga cggcacggtg
     2761 gtggaggtga accacgccgc tgaaattctg ttcggctacg cggccgccga gctcgtcggc
     2821 ggccccgtga agaagctcgt gctgcccaag tttcacgagg acttcgacca tacgtggcag
     2881 cagctcgtca cttcgcgcga atcggtggcc gggctcaaga tccgcaaccc gcgccgcgac
     2941 ggcgccgaga tcgtctgcga atggaccgtc accccgctcg tcaacttcca gcgggaggtc
     3001 attgcggtga tcgcccaggg gcgcgacatt accgcgcagc tcgaggccga gcgcatgaag
     3061 aaggagttca cctcgacgct ttcccacgag ctgcgcacgc cgctcacctc gatcatcggc
     3121 tcgctacagc tcatcaatgg cggcgtgatg ggcgaggtgg acaaggaggt ggccgagctc
     3181 accatggtcg ccgagcgcaa cggtcagcgc ctgctcgacc tcatcaacga catcctggac
     3241 atcgagaaga tcgaatcggg caagctcacc ctggcgcctg agctcatcgc gctcgacgag
     3301 ctggtgcgcg agtcgatggt gctgaacaag gccttcggcg agcgcttcca ggtgcgcttc
     3361 gaggcgcacg gcaggatgtc caacacccgg gtgcacgccg accgcaagcg cctgctccaa
     3421 gtgatgacca acctgctctc gaacgcggcc aagttctcgc ccgagggcgg cgtggtagag
     3481 atcagcaccg cggacgttgg gccgagcgtg cgcgtttcgg ttcacgaccg cggcagcggc
     3541 atcccggaag cgttccgcca gcgcattttc ggtcgcttca cccaggccga ttcgaccgcc
     3601 acgcggcaga agggcggcac cggcctcggc ctcgccattt gcaggcggct gctcgagctg
     3661 atgcacggcc gcatcggctt tgacgaccgc ccgggcggcg gcaccacatt ctggttcgag
     3721 ctgccgcgcc acagcgcctg agatcgaaga ccccatgcca gaccggcctc tcaaccgcat
     3781 ctgctacgtc gaggacgacg aagacatcca gcgcatcgtg cgcatg
//
LOCUS       DASUNT010000497         2513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_156644, whole genome shotgun sequence.
ACCESSION   DASUNT010000497 DASUNT010000000
VERSION     DASUNT010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156644"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFX94_10030"
     CDS             <1..264
                     /locus_tag="VFX94_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX5768560.1"
                     /translation="EEVIAELVQVRGIGRWTAEMFLIFNLLRPDVLPLDDLGLQKAIR
                     LRYFRGRKVSPARMRRLGEQWQPWRSVATWYLWRSLDPVPVEY"
     gene            272..1240
                     /locus_tag="VFX94_10035"
     CDS             272..1240
                     /locus_tag="VFX94_10035"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX5768561.1"
                     /translation="MKTTFLEFEQPIAELENRIEELRYVQDDSALDISEEIERLTKKS
                     QALTKEIYAKLTPWQVAQVARHPQRPFTLDYIQGLFTHFEELHGDRTFSDDASIVGGL
                     ARFNGAPCVVLGHQKGRDTKEKIARNFGMPKPEGYRKALRLMKLAEKFGLPVFTFVDT
                     PGAYPGIDAEERGQSEAIGRNLFEMARLRTAIIVSIIGEGGSGGALAIAVGDVILMMQ
                     YATYSVISPEGCASILWRSAEMAPDAADALGITASRLKTLGLVDKIVPEPLGGAHRDP
                     QAAAQALKKALAEALRQVQDKKPKELVETRLERLMAHGKFKEATER"
     gene            1209..2378
                     /gene="tilS"
                     /locus_tag="VFX94_10040"
     CDS             1209..2378
                     /gene="tilS"
                     /locus_tag="VFX94_10040"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX5768562.1"
                     /translation="MASSRKRPSGSARIAVGLSGGIDSVVLLHRLRDVPGISAIHVHH
                     GLSPNADAWAAFCRRLCKRLDVPLTVRKVKVVKRGGGLEAAARAARYAAFRKIPVNVV
                     ALAHNLDDQAETVLMNLLRGAGLRGASGMARESRFEGKKLLRPLLDTSRNEIEAYARR
                     HGLEWIEDESNADESLTRNFIRRRVGPLLEERYPRWRENLARSARHFSRKEAQAQYLL
                     RKYLSAKGLRAPTEAKLVEMLKQLTGAGSRTLIEHDGARLRVYRGKILHDRAAHSFSS
                     IPWKGEARLPIPELGGELRFRTARGKGIAASARPLRVRLRSGGERLQPDARRPRRTLK
                     NLFQEAGVPPWERDRLPLLFSGEDLVWAPGLGIDARYQAGKNAFGVLPEWRRIAP"
     gene            2409..>2513
                     /locus_tag="VFX94_10045"
     CDS             2409..>2513
                     /locus_tag="VFX94_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX5768563.1"
                     /translation="MAVEQTLSIIKPDAVKKNVIGQILARFETAGLRIV"
BASE COUNT          443 a          800 c          876 g          394 t
ORIGIN      
        1 gaggaggtga tcgcggagct cgtccaggtg cgtggaatcg gtcgctggac cgcagagatg
       61 ttcctgatct tcaacctgct gcggccggac gtgctgccgc tcgacgacct cgggctgcag
      121 aaggcgattc gcctgcgcta cttccgcggc cgcaaggtgt cgccggcgcg catgcggcgg
      181 ctcggggagc agtggcagcc gtggcgctcg gtggcgacct ggtacctttg gcgctcgctc
      241 gatccggtgc cggtagaata ctgatccggg gatgaagacc acgttcctcg aattcgagca
      301 gccgatcgcg gagctcgaga atcgcatcga ggaattgcgc tacgtccagg acgattccgc
      361 cctcgacatc tccgaggaga tcgagcggct gacgaagaag agccaggcgc tcaccaagga
      421 gatctacgcc aagctcacgc cctggcaggt ggcgcaggtc gcgcgccatc cgcagcggcc
      481 cttcacgctc gactacatcc agggactgtt cacccatttc gaggaactgc acggcgaccg
      541 cacgttttcc gacgacgctt cgatcgtcgg cgggctcgcg cgcttcaacg gcgcgccctg
      601 cgtggtgctc ggtcatcaga aagggcgcga caccaaggag aagatcgcgc gcaatttcgg
      661 tatgcccaag cccgagggct accgcaaggc gctgcgtctc atgaagctcg ccgagaagtt
      721 cggcctgccg gtgttcacct tcgtcgatac gccgggcgcc tatcccggca tcgacgccga
      781 ggagcgcggc cagtccgagg ccatcgggcg caacctcttc gagatggcgc gcctgcgcac
      841 ggcgatcatc gtatcgatca tcggcgaggg cggctcgggc ggcgcgctcg ccatcgcggt
      901 cggcgacgtc atcctgatga tgcagtacgc cacctactcg gtgatctcgc ccgagggctg
      961 cgcttcgatc ctctggcgca gcgccgagat ggcgcccgac gccgcggacg cgctcggcat
     1021 cacggcgagc cgcctcaaga cgctcggcct ggtggacaag atcgttcccg agccgctcgg
     1081 cggtgcgcac cgcgatccgc aggccgcggc gcaggcgctg aagaaggcgc ttgccgaggc
     1141 gctcaggcag gtgcaggaca agaagccgaa agaactcgtc gagacccggc tcgagcgcct
     1201 gatggcccat ggcaagttca aggaagcgac cgagcggtag cgcgcgcatc gccgtcgggc
     1261 tgtcgggcgg cattgactcg gtggtattgc tgcacaggct gcgcgatgtc ccggggattt
     1321 ccgcgatcca tgtccatcac gggctgagcc cgaacgcgga cgcctgggcg gctttctgcc
     1381 gccggctgtg caagcgcctg gacgtgccgc tcacggtgcg aaaggtgaag gtggtaaagc
     1441 gcggcggcgg actcgaagcg gcggcgcgcg ccgcccgcta tgcagccttc agaaaaattc
     1501 cagtcaacgt cgtagcgctg gcgcacaacc tggacgacca ggccgagacc gtgctcatga
     1561 acctgctgcg cggcgccggg ttgcgcgggg cgagcggtat ggcacgcgag tcgcggttcg
     1621 agggaaagaa gctgctgcgg ccgctgctcg atacttcgcg caacgagatc gaagcttatg
     1681 cgcgccgcca cgggctcgag tggatcgagg acgagagcaa cgcggacgag tcgctgacgc
     1741 gaaacttcat ccggcggcgc gtcggccctc tgctggaaga aagatatccg cgatggcgcg
     1801 agaacctggc gcgctcggcg cggcatttct cgcgaaagga agcgcaggcg cagtatctgc
     1861 tgagaaaata cctgagcgcg aagggactgc gtgcgccgac cgaggcaaag ctggtcgaga
     1921 tgctgaagca gctcactggc gcaggcagcc gcacgctgat cgagcacgat ggcgcgcggc
     1981 ttcgcgtcta ccggggaaaa atcctccacg accgcgccgc gcactcgttc agctcgattc
     2041 cgtggaaggg cgaagcgcgg ctgccgatcc cggagctcgg cggggagctt cgctttcgga
     2101 ccgcccgcgg caagggcatc gcggcgagcg cgcggccgct gcgagtgcga ctgcgctccg
     2161 gcggcgagcg cctgcagccc gacgcgcgcc ggccgcggcg cacgctgaag aaccttttcc
     2221 aggaagcggg cgtgccgccg tgggagcgcg accgcctgcc gctccttttc agcggcgagg
     2281 atctcgtctg ggcgcccggc cttggcatcg acgcacgcta tcaggcgggc aaaaatgctt
     2341 tcggcgtgct gccggaatgg cgtagaatcg cgccttaatt tcttgaattt acaaggagaa
     2401 tgtagttcat ggcggtggag cagacgctct cgatcatcaa gccggacgcg gtgaagaaga
     2461 atgtgatcgg gcagattctc gcgcgcttcg agaccgcagg cctgcgcatc gtg
//
LOCUS       DASUNT010000498         3263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_244047, whole genome shotgun sequence.
ACCESSION   DASUNT010000498 DASUNT010000000
VERSION     DASUNT010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244047"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VFX94_10050"
     CDS             complement(<1..543)
                     /locus_tag="VFX94_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5768564.1"
                     /translation="MPVDLQGIPLFQGVSEADLRALADRTAVRSYPKQAIILSEGDES
                     DSLYLILAGRVKIYLADEHGKELILAIKGPGQYFGEMVLDGDARSASVMTLEPAQFAI
                     LSRADFRAFLLGHAAVALQLVENLIRVARGLNRSVRNLAMLDVYGRVARILLELAVER
                     DGKLVIPERMTQKDIAARVGA"
     gene            591..1202
                     /locus_tag="VFX94_10055"
     CDS             591..1202
                     /locus_tag="VFX94_10055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5768565.1"
                     /translation="MPVHRLTRALAHQLARRGRLHILRVFSRPLRVPARPPAPPPGLE
                     LRSVSAAELRRHSADPALELREPMIADVLGRGDLCLGALEGGILAGYVWLAYETAPHV
                     HGIRVQVPPRAVYLFKSFIRPSHRGRGIAAALYRAADALAARPGRELVVSCVEAHNHP
                     SIALSLRDGSRPLGALAYWRAGPWYAAFHSAAVRRLGLRFYLA"
     gene            complement(1533..2153)
                     /locus_tag="VFX94_10060"
     CDS             complement(1533..2153)
                     /locus_tag="VFX94_10060"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5768566.1"
                     /translation="MHIEAVKAAYRRYARIYDVVFGAVLQPGRRAVLEALNLSPGDRV
                     LEVGVGTGISLPLYPRDVRITGIDVSREMLEKARARVARARLTNVDALLEMDAEAMAF
                     PDASFNKVVAMYVVSVVPRPAKLLEELHRVCRPDGEIFIVNHFQSENPIIGRVERALA
                     GFSSQIGFNPDLDLRELVPAAQNGEVSRVNFFWRMVRLRNGVSHKL"
     gene            2219..>3263
                     /locus_tag="VFX94_10065"
     CDS             2219..>3263
                     /locus_tag="VFX94_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006415729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5768567.1"
                     /translation="MKIAQVAPLAESVPPRLYGGTERVVSYLTEELVRLGHDVTLFAT
                     ADSQTAARLVACAPRALRLDPEATDALPHHLLMIERVRSLAHEFDVLHFHVEHVHLPL
                     FRPFAHKCVTTLHGRLDLPDLVPLYREFREMPLVSISNAQRRPLAGANWVGTVYHGLP
                     DAVCPYNPAPRGDYLAFLGRISPEKGIEHAIEVAHRAGRRLRIAAKIDSVDEAYWHSR
                     VKPLIGGPIEFVGEVSEEDKPSFLGNATALLFPIDWPEPFGLVMIEAMSCGTPVIAWP
                     RGSVPEIVDHGVTGFITRSVDEAVAEVGRAARLDRARVRRRLEERFSATRMAVDYLAV
                     YRSLGVKQRTELVP"
BASE COUNT          493 a         1141 c         1077 g          552 t
ORIGIN      
        1 ggcgccgacg cgggccgcga tgtccttctg cgtcatgcgc tcggggatca ccagcttgcc
       61 gtcgcgctcg accgcgagct cgagcaggat gcgcgcgacc cgcccgtaca cgtccagcat
      121 ggcgaggttg cgcacgctgc ggttcagccc gcgcgccacc cgtatcaggt tctcgacgag
      181 ctgcagcgcg accgcggcgt gcccgagaag gaaggccctg aagtcggcgc gtgacaagat
      241 cgcgaactgc gccggctcga gcgtcatcac cgaggcggac cgggcgtcgc cgtcgagcac
      301 catctcgccg aagtattgcc ccggcccctt gatcgcgagg atcagctcct tgccgtgctc
      361 gtcggcgaga tagatcttca cccggccggc cagaatcagg tacagcgagt cgctctcgtc
      421 gccctcgctc aggatgatgg cctgcttcgg atagctgcgc accgcggtgc ggtcggcgag
      481 cgcgcgcaag tcggcttccg aaacgccctg gaaaagcggt atgccctgga ggtcaaccgg
      541 catggggtgg cgcgggggat tctagggttg gccgcggcta ggctacaagg gtgcctgttc
      601 accggctgac ccgggcgctc gcccatcaac tcgcccgccg cggccgcctg cacatcctgc
      661 gcgtcttctc ccggccgctg cgtgtgccgg cgcgtccgcc tgcccctccg ccggggctcg
      721 agctgcgctc ggtctcggcg gctgagctgc gccgtcacag cgccgacccc gcgcttgagc
      781 tgcgcgagcc gatgatcgcc gatgtcctcg ggcgcggcga cctgtgcctc ggcgcgctcg
      841 agggcggcat ccttgccggt tatgtctggc tcgcctacga aaccgcgccg cacgtgcacg
      901 gtatacgggt ccaggtgcca ccccgggccg tgtatctctt caagagcttt atccgcccgt
      961 cccatcgcgg gcgcgggatc gcggctgcgc tttaccgggc cgccgacgct ctcgcggcgc
     1021 ggccgggccg cgagctggtg gtgagctgcg tcgaagccca caaccatcct tccattgcgc
     1081 tctcgctgag ggacggcagc cgaccgctcg gcgcgctcgc gtactggcgc gccggcccgt
     1141 ggtacgccgc cttccattct gcggccgtgc gccgcctggg gctgcgcttc tacctcgcct
     1201 gattggtttt tttcagctgg cgctcgagcg cgccgaaggc gaggttgatg agcgccggca
     1261 gctccgtcgg catgtcaccg atgaccttgg tcttgcgccc gcaagatcct tgttgggcgg
     1321 catgcgagcc tcgaccagca tgctgtagcg gttgcccttg tgctgcgagg cctgctcctg
     1381 cgtaagcgaa tcccggcacg acgtgatacc gttcgcgaac ttctccagcc gctacaagcg
     1441 ctcgagaatg tagttctcga cacaacgggg acggagccct aattccgtag ctccgcgaag
     1501 tgaattaggg ctccgtcccc cgaatttcgc gtttacaact tgtgcgaaac tccgttccgc
     1561 aggcgcacca tccgccagaa gaagttgacg cgcgagacct cgccgttctg cgccgcaggc
     1621 acgagctcgc gcaggtccag gtcggggttg aagccgatct gggaggaaaa gcccgccagc
     1681 gcgcgctcga ctctcccaat gatcgggttc tcggactgaa aatggttgac gatgaagatc
     1741 tcgccgtccg gccggcagac gcgatgcagc tcttccagca gcttggccgg ccgcggcacg
     1801 accgagacca cgtacatcgc caccaccttg ttgaagctcg cgtcggggaa cgccatcgcc
     1861 tcggcgtcca tctcgagcag cgcgtcgacg ttggtgagcc ttgcgcgcgc gacgcgggcg
     1921 cgggctttct ccagcatctc gcgcgacacg tcgatcccgg tgatgcgcac gtcgcgggga
     1981 tagagcggca acgaaatgcc ggttccgacg ccaacctcca gaacgcggtc gcccgggctg
     2041 agattcaggg cctccagcac tgcgcggcgg cccggctgca gcacggcgcc gaacaccacg
     2101 tcgtagatcc tcgcatagcg ccggtaggcc gccttgacgg cttcgatatg catgagttgg
     2161 ccgattctgc cagcaatcgg cattcccggc acgcgcattg caaccgtgtt tcgaggacat
     2221 gaagatcgca caggtagcac ccttggcgga gagcgtgccg ccgaggctct acgggggtac
     2281 cgagcgggtc gtctcctacc tcaccgaaga gctggtaagg ctggggcacg acgtgaccct
     2341 gttcgcgacc gccgactcgc agacggctgc gcgccttgtc gcctgcgcgc cgcgcgcgct
     2401 gcgcttagac ccggaagcca ccgacgccct gccgcaccac ctgctcatga tcgagcgggt
     2461 ccgcagcctc gcgcacgaat tcgacgtgct gcacttccac gtggagcacg tgcatctgcc
     2521 gttgttcagg cccttcgcgc acaagtgcgt caccacgctg cacggcaggc tggacctgcc
     2581 cgacctcgtg cccctgtacc gggagttccg cgagatgccg ctggtgtcga tctccaacgc
     2641 gcagcgccgg ccgctcgccg gcgcaaactg ggttggcacc gtgtaccacg ggctgcccga
     2701 cgccgtgtgc ccgtacaacc cggcgccacg cggggactac ctcgcgtttc tcggccgcat
     2761 ttcgcccgag aaaggaatcg agcacgcgat cgaggtcgcg caccgcgccg gccggcggct
     2821 gcgcatcgcg gcgaagatcg acagcgtcga tgaagcttac tggcacagcc gtgtcaagcc
     2881 gctcatcggc ggcccgatcg agttcgtggg cgaagtgagc gaggaagaca agccttcgtt
     2941 cctcggcaat gcgacggcgc tgctcttccc gatcgactgg cccgagccct tcggcctggt
     3001 gatgatcgag gcgatgtcct gcggcacgcc cgtgattgcc tggccgcgcg gctcggtgcc
     3061 cgagatcgtg gaccacggcg tcaccggctt catcacgcgc tcggtcgacg aggcggtcgc
     3121 cgaggtcggc agggctgctc gcctggaccg tgcccgcgtg cgccggcgcc tggaggagcg
     3181 cttcagcgcg acacggatgg cggtcgacta cctggcggtg taccgaagcc tcggcgtcaa
     3241 gcaaagaacc gaactcgttc cgt
//
LOCUS       DASUNT010000499         2259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_332622, whole genome shotgun sequence.
ACCESSION   DASUNT010000499 DASUNT010000000
VERSION     DASUNT010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332622"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VFX94_10070"
     CDS             <1..861
                     /locus_tag="VFX94_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5768568.1"
                     /translation="LIPFGLAMCGPVLLKFGSEAQKKRFLPRIYNGDDFWCQGYSEPG
                     SGSDLASLKTKAEKKENHYVVNGQKTWTSYAHYADWIFCLVRTDPTVEKRQEGISFLL
                     MDMRSPGITVRPLILMDGYHAVNEVFFDDVKVPLENLVHKENQGWTVAKYLLGYERMN
                     TGRIGESKRELRKIKEMNNEKTRDLRFRDRLTRVEVELMALEITNLRFLDRMRRTGQP
                     PGADVSMLKIKGTEIQQALTELAIQAADPNARETQALERRYLDMRKTTIYAGSNEIQR
                     NIIAKMTLGL"
     gene            858..1844
                     /locus_tag="VFX94_10075"
     CDS             858..1844
                     /locus_tag="VFX94_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5768569.1"
                     /translation="MNFDYNEEQQLLADSVRRYLQKDYDFEARKKILASAEGWSEPVW
                     STFAEMGLTGLPLSPDYGGFGGGAVDLMSVMEAFGEALVVEPYLPTVLAALAIAKGGE
                     ARQKQEILPLVAQGKMRLALATGPRGVIGAPMADRIVFGSEGRLRIVARDAIEVRAYR
                     TVDDLAAGDIRLEGKGEPLAGGVLDEVLDFGAALSCAEAVGAMKFACDTTLEYLKTRR
                     QFGVPIGAFQALQHRMVDMFMSYEQAKSMACLACSKVDQAESARDRARSVSAARIRIA
                     DAARHISQESVQLHGGMGMSEELKVSHTFRRLTALGRRFGDADHHLARFASL"
     gene            1831..>2259
                     /locus_tag="VFX94_10080"
     CDS             1831..>2259
                     /locus_tag="VFX94_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768570.1"
                     /translation="MPRYKGWIAAALFVAACAEQPAPKEEVTPPPPDLPPLPASFVHE
                     PECPGCLAVTLTLRPDGAYLLRDQLGASEFYDFGRWRYAEGTLELAGDRDTRRYAFKD
                     LRHASRVEPLRGPFRMVGLYDGTSFKECRTGLAWSLADTRA"
BASE COUNT          407 a          740 c          750 g          362 t
ORIGIN      
        1 ctgattccgt tcggcctcgc catgtgcggc ccggtgctgc tcaagttcgg cagcgaggcg
       61 cagaagaagc gcttcctgcc gcgcatctac aacggcgacg atttctggtg ccaggggtac
      121 tccgagccgg gctccggcag cgatctcgct tcgctcaaga caaaggcaga gaaaaaagag
      181 aaccattacg tcgtcaacgg ccagaagacc tggacctcct acgcccacta cgccgactgg
      241 atcttctgcc tggtgcggac cgatccgaca gtcgagaagc gccaggaagg gatctcgttc
      301 cttctcatgg atatgcgctc gcccgggatc acggtgcggc cgctcatcct catggatggg
      361 taccacgcgg tgaacgaggt gttcttcgat gacgtgaagg ttccgctcga gaacctcgtg
      421 cacaaggaga accagggctg gacggtggcg aagtacctgc tcggctacga gcgcatgaac
      481 accggccgca tcggcgaatc gaagcgcgaa ctgcgcaaga tcaaggaaat gaacaatgaa
      541 aagacgagag accttcgctt ccgggatcgc cttacgcgcg tcgaagtgga gctgatggcg
      601 ctcgagatca cgaacctgcg cttcctcgac cgcatgcgcc gtaccggcca gccgccgggc
      661 gcggacgtat cgatgctgaa gatcaaaggc acggagatcc agcaggcgct caccgagctc
      721 gcgatacagg ctgccgatcc gaacgcgcgc gagactcagg cgctcgagcg gcgctatctc
      781 gacatgcgaa agaccacgat ctatgccggc tcgaacgaga tccagcgcaa catcatcgcc
      841 aaaatgacgc tgggtctatg aacttcgatt acaacgaaga gcagcagctt ctcgccgact
      901 cggtgcgccg ctacctgcag aaggactacg atttcgaggc gcggaagaaa attctcgcct
      961 cggccgaggg ctggagcgag cccgtgtggt cgacgttcgc ggaaatgggc cttaccggcc
     1021 tgccgctctc gcccgactat ggcgggttcg gcggcggcgc ggtcgacctg atgagcgtaa
     1081 tggaagcgtt cggcgaggcg ctcgtggtcg agccgtatct tccgaccgtg ctcgccgccc
     1141 tcgcgatcgc gaagggcggc gaggcgaggc aaaagcagga gatcctgccg ctcgtggcgc
     1201 agggcaagat gcggcttgcg ctcgccaccg gcccgcgcgg cgtgatcggc gcgccgatgg
     1261 ccgatcggat cgtgttcggc tccgagggga ggctgcgcat cgtcgcgcgc gacgcgatcg
     1321 aagtgcgcgc ttaccggacc gtcgacgacc tcgcggcggg cgacatccgc ctggagggca
     1381 agggcgagcc gctcgccggc ggcgtactcg atgaggtgct cgattttggc gccgcactct
     1441 cctgcgcgga ggcggtgggg gcgatgaagt tcgcatgcga caccaccctc gaatacctga
     1501 agacgcgccg gcagttcggc gtgccgatcg gcgcgttcca ggcgctgcag caccggatgg
     1561 tggacatgtt catgtcctac gagcaggcga agtccatggc ctgcctcgcg tgctcgaaag
     1621 tggaccaggc cgaaagcgcg cgcgaccgcg cccgctcggt ctcggcggca agaatcagga
     1681 tcgcggacgc agcgcgccac atcagccagg aatccgtgca gctccacggc ggcatgggca
     1741 tgagcgagga gctgaaggtc agccatacct tccggcgcct caccgcgctc ggccgccgct
     1801 tcggcgacgc cgatcaccac ctcgcgcgtt ttgcctcgct ataaaggctg gatcgccgcg
     1861 gcgcttttcg tcgccgcgtg cgccgagcag cctgctccaa aggaagaggt gacgccgccg
     1921 ccgccggatt tgccgccgct gccggcgagc ttcgtccatg agccggagtg ccccggctgc
     1981 ctcgcggtta cgctgaccct gcgccccgac ggcgcttacc tgctgcgcga ccagctcggc
     2041 gcctcggagt tctacgactt cggacgctgg cgctacgccg agggcacgct cgagctcgcg
     2101 ggcgatcgcg atacgcgccg ctacgctttc aaggatctca ggcacgcctc gcgggtcgag
     2161 ccgctgcgcg gcccgtttcg catggtcggc ctctacgacg gcacgtcgtt caaggagtgc
     2221 cgcaccggcc tcgcctggag cctcgccgat acgcgtgcg
//
LOCUS       DASUNT010000500         2344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1120232, whole genome shotgun sequence.
ACCESSION   DASUNT010000500 DASUNT010000000
VERSION     DASUNT010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120232"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="VFX94_10085"
     CDS             complement(<1..1132)
                     /locus_tag="VFX94_10085"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5768571.1"
                     /translation="MTPSAAHAAREIREGRLTSEELVHACLERIRALEPKVQAWQFLD
                     EEHALAQARTLDERKRSGEPIGPLHGVPVGIKDIIDTADMPTENGTVLHKGRTPREDA
                     AVVKMLRAAGAVILGKTVTTECAYFNPGKTRNPHNPEHTPGGSSSGSAAAVGAGMVPL
                     ALGSQTAGSVIRPAAFCGAYGFKPTHGLIPRTGILALSRTLDHVGLFARSVEDLALLL
                     EQLQGYDEQDADTSPRARIPFSMVCGEEPPLEPMFAFIKTPHWERTDPDTKEAYAELQ
                     ETLGKQVEEVELFPSAAEAWDWHRTIMECEMAANLEPLWLAGREKLSDKLRALLERGR
                     EARALDYQRALRRIAPAVESLDELFQQRYDAILTPPALGTAPK"
     gene            complement(1129..>2344)
                     /locus_tag="VFX94_10090"
     CDS             complement(1129..>2344)
                     /locus_tag="VFX94_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX5768572.1"
                     /translation="NGLLRAALGGSGVRIPTVYTQLLITALPILAIAGILLVTYRSST
                     APVVEASAAGLIEAGGAFAAEAGSQEQSAGLIGAPESEEETKEEEEKPQELSAPPGAE
                     EQKPAAEAQPAAEVKPAAAPAAAEPERRPLPTWYYVVLAIALALALVLYWAWTWERLQ
                     VFKMLLASFFPLALLILAVLGSIVLGLATPAEAAAVGAFGAFILTAVYRFVAQWGERA
                     QTRASPGEVVWRTLKQFGSIIKESSFLTAKTSAMVCWLFVGSGIFSAAFALLGGQELL
                     EKWVLSLNLSPIQFMILAQIIIFILGWPLEWTEIIVIFMPIFIPLLPHFGIDPLFFGL
                     LVALNLQTAFLSPPVAMAAFYLKGVSPPHVSLNAIFAGMLPFMGIQVIALILLYTFPG
                     IGLWLPNVFYGR"
BASE COUNT          401 a          780 c          800 g          363 t
ORIGIN      
        1 ccttcggcgc ggtgccgagc gcgggcggcg taaggatggc gtcgtagcgc tgctggaaaa
       61 gctcatcgag gctctccacc gcgggcgcga tgcgccgtag cgcgcgctgg tagtcgagcg
      121 cgcgcgcttc gcggccgcgc tcgagcagcg ccctcagctt gtcggaaagc ttctcgcgcc
      181 ccgccaacca gagcggctcg aggttcgccg ccatctcgca ttccatgatc gtcctgtgcc
      241 agtcccacgc ttcagcggcg gaaggaaaga gctcgacctc ctccacctgc ttgccgagcg
      301 tttcctgcag ctctgcgtac gcttccttgg tatccgggtc ggtgcgctcc cagtgcggcg
      361 tcttgatgaa ggcaaacatc ggctcgagcg gcggttcttc accgcagacc atggagaaag
      421 gaattcttgc cctgggactc gtatcggcat cctgctcgtc gtagccctga agctgctcga
      481 gcagtagcgc gagatcctcg acgctgcgcg cgaaaagacc cacatggtcg agcgtgcggg
      541 agagcgccag gatgcccgtg cgtggaatga ggccgtgcgt aggcttgaac ccgtaggcgc
      601 cgcagaaggc cgccggccgg atcaccgagc ccgcggtctg gctgccgagc gcgagcggca
      661 ccatgccggc cccgactgcg gcggccgagc cgctcgagga gccgccgggc gtgtgctcgg
      721 ggttgtgcgg attgcgggtt ttccccggat tgaagtaggc gcactcggtg gtcacggtct
      781 tgccgaggat caccgcgccc gcggcgcgca gcatcttgac caccgccgcg tcctcgcgcg
      841 gcgtgcggcc cttgtgcagg accgtgccgt tctcggtcgg catgtccgcg gtgtcgatga
      901 tgtccttaat cccgaccggc acgccgtgca acggcccgat cggctccccc gagcgcttgc
      961 gttcgtcgag cgtccgagcc tgggcgagcg catgctcttc gtcgaggaat tgccaggcct
     1021 ggaccttggg ctcgagcgcg cgaatgcgct cgaggcaggc gtggaccagc tcttcggagg
     1081 tcagcctgcc ttcacgaatc tcgcgcgcgg catgcgcggc ggacggcgtc atcggccgta
     1141 gaacacgttc ggcagccaga gcccgatgcc ggggaaggtg tagagcaaaa taagcgcgat
     1201 gacctggatg cccatgaacg gcagcatgcc ggcaaaaatt gcgttgagcg agacatgcgg
     1261 cggcgacacc cccttgagat agaacgccgc catcgccacc ggcggcgaca ggaacgcggt
     1321 ctgcaggttc agtgcgacga gcaggccgaa gaacagcgga tcgatgccga agtgcggcag
     1381 cagcggtatg aagatcggca tgaagatcac gatgatctcg gtccactcga gcggccaccc
     1441 caggatgaag atgatgatct gggcgaggat catgaactgg atcggcgaga gattcagcga
     1501 cagcacccac ttttcgagca gctcctgccc gccgagcagc gcgaacgccg ccgagaagat
     1561 gcccgagccg acgaagagcc agcacaccat cgcgctcgtc ttggcggtaa ggaacgatga
     1621 ttccttgatg atcgagccga actgcttcag agtgcgccag acaacttcgc ccggcgaggc
     1681 tcgcgtttgg gcgcgctcgc cccactgcgc gacgaagcgg tagacggcgg tcagtatgaa
     1741 ggcgccgaaa gcgcccaccg cggcggcctc ggccggcgtc gccaggccga ggacgatgga
     1801 gccgagcacc gcgaggatga gaagggcgag cgggaaaaag gaggcgagca gcatcttgaa
     1861 gacctggagc cgctcccagg tccaggccca gtacaggaca agcgccagcg ccagcgcgat
     1921 cgccagcacg acgtaatacc acgtgggcag ggggcggcgc tcgggctcgg cggcggctgg
     1981 cgccgcggcc ggcttgacct cggccgccgg ctgggcttcg gcggcgggct tctgctcctc
     2041 cgcgccaggc ggcgctgaca gctcctgcgg cttttcctct tcctccttcg tttcctcctc
     2101 gctctcgggc gcgccgatca gcccggcaga ctgctcctgc gagcccgctt ccgccgcgaa
     2161 cgccccgccc gcctcgatca ggccggctgc actcgcctcc accaccggcg cagtcgagct
     2221 tcgataggtc accaacagaa tgcccgcgat ggcgagtatc ggcagcgcgg tgatcaagag
     2281 ctgcgtgtaa accgtcggta tgcgcacgcc cgagccgccg agcgcggccc gcaagagacc
     2341 gttg
//
LOCUS       DASUNT010000501         2539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1120248, whole genome shotgun sequence.
ACCESSION   DASUNT010000501 DASUNT010000000
VERSION     DASUNT010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120248"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /gene="rfbB"
                     /locus_tag="VFX94_10095"
     CDS             complement(<1..829)
                     /gene="rfbB"
                     /locus_tag="VFX94_10095"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX5768573.1"
                     /translation="MILVTGGAGFIGSNFVLSTLKERDEPILNLDKLTYAGRLSNLEL
                     LRDDARHIFVQGDICDRGLVRELLQRHKPRAIVHFAAESHVDRSISGPAPFIETNVTG
                     TFCLLEEARSYSRDIRFLHVSSDEVYGSLRPDDPPFTEDTPYAPNSPYAASKAASDHL
                     VRAYHQTYGLPTLTTNCSNNYGPRQFPEKLIPLTIANALSGKPLPVYGDGRNVRDWLY
                     VEDHCEAVRLVLERGRPGETYNIGGSCEKRNIDVVRTICALLDDAKPRKAGKYAALIS
                     "
     gene            862..2265
                     /locus_tag="VFX94_10100"
     CDS             862..2265
                     /locus_tag="VFX94_10100"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEX5768574.1"
                     /translation="MPFVPVILCGGAGTRLWPLSRSHLPKQFLPLVSERTMLQETVAR
                     LQGLEGALSPIAIANDEHRFLAAEQLREVSCAPQALILEPAGRGTAAAAAAAALLLPD
                     RESVLLVLAADHAISNLPQFHAAIGSATRLAGEGALVVFGIAPASPHTGYGYIKQGAR
                     RADAYIVERFVEKPDVETAKGFLASGGYFWNSGMFAFRADRYLAELERYRPDIVAGVK
                     RAMATAKRDMDFVRPDPPAFLAVPDDSIDRAVMEKTRDAVVVPAKFDWSDVGSWSALW
                     ELGAKDALGNVSQGDVMARDSHGSYLRSEGRLVAAVGVENLVVIETSDALLVSTRERA
                     DDVKLAVEQLKRGRRTEHAIHRRVYRPWGYYESIDSGESFQVKRLMVKPGARLSLQKH
                     KRRAEHWVVVSGEALVTRGEEELRLRANQSTYIPTGTKHRLENVGADPLFVIEVQSGD
                     YFGEDDIERFADDYRRA"
     gene            complement(2250..>2539)
                     /locus_tag="VFX94_10105"
     CDS             complement(2250..>2539)
                     /locus_tag="VFX94_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="heptosyltransferase"
                     /protein_id="HEX5768575.1"
                     /translation="IAHLARLVGVPTVALYGPGSPVSTGPGRFWRTSPFRALWDPEVA
                     CRDQDRLFERRLVWLRQCWRSVEECGDPICIRRVAVAQVVAAIEQLTQARR"
BASE COUNT          444 a          790 c          863 g          442 t
ORIGIN      
        1 agctgatgag cgcggcgtac ttgccagctt tgcggggctt tgcgtcgtcg agaagcgcgc
       61 aaatggtacg taccacgtcg atgttccgct tctcgcagct gccgccaatg ttgtacgttt
      121 caccgggtcg tccgcgctcg agcaccaggc gtacagcttc gcaatggtct tcgacataga
      181 gccagtcgcg cacgttccgg ccgtcgccgt acaccggaag cggcttgccg gacagcgcgt
      241 tggcaatggt gagcgggatc agcttttcgg gaaactggcg ggggccatag ttgttcgagc
      301 agttcgtggt cagcgtcggc aagccgtagg tttggtggta ggcccgcacc aggtggtctg
      361 acgcggcctt ggacgctgca tacgggctgt tcggcgcgta cggagtgtcc tcggtgaagg
      421 gtggatcgtc aggacggagg gagccgtaga cctcgtccga ggagacatgc aggaatcgga
      481 tgtcgcgcga atatgagcgg gcttcctcga ggaggcaaaa ggtcccggta acgttggtct
      541 cgatgaacgg cgccgggccc gaaatggagc ggtcgacgtg gctctcggcc gcgaagtgca
      601 cgatcgctct tggcttgtgt ctctgcagta gctctctgac gagcccgcgg tcacaaatgt
      661 cgccctgcac gaagatgtgg cgcgcatcgt cgcgcaacag ctccaggttg ctcagcctcc
      721 ccgcgtaggt cagcttgtcg agattcaaga tcggctcgtc gcgctctttg agcgtcgaaa
      781 gcacgaaatt cgagccgatg aagcccgccc cacccgtaac caggatcatt ggattcaggg
      841 caattctata attgcccgca catgccattc gtgccggtga ttctgtgcgg gggcgcggga
      901 actcgcctgt ggcctctgtc ccgctcgcat ctgccaaagc agttcctgcc gctcgtctcc
      961 gagcgcacta tgctgcagga gactgtggcg cgtttgcagg gcctcgaggg cgcactgtcg
     1021 ccgatcgcga tcgcgaacga cgagcatcgc ttcctcgccg ccgagcagtt gcgcgaggtt
     1081 tcatgcgcgc cgcaggcgct gatcctcgag cccgccggca ggggtaccgc agcggccgcg
     1141 gccgccgccg ccttgttgct gcccgacagg gagagcgtgc tgctagtgct ggccgcggac
     1201 cacgctatct ccaacctgcc gcagttccat gcggcgatcg gctcggccac gcgcctggcc
     1261 ggtgaagggg cgctcgtggt attcggcatt gcgccagcga gcccacatac cggctacggc
     1321 tacatcaagc agggagcacg gcgcgccgac gcgtacatcg tcgagcgctt cgtcgaaaag
     1381 cccgatgtcg agaccgcgaa gggctttcta gcatccggcg gctacttctg gaacagcggc
     1441 atgttcgcgt ttcgtgccga ccgctacctc gccgagctgg agcgctatcg gcccgacatc
     1501 gttgccggcg tcaaacgggc aatggcgacg gcaaagcgcg acatggactt cgtgcgcccg
     1561 gaccccccgg cgttcctcgc agtgcctgac gactcgatcg atcgcgccgt gatggagaag
     1621 acgcgcgacg cggtggtagt accggcgaag ttcgactgga gcgacgtcgg ctcgtggagc
     1681 gcgctgtggg agctcggcgc caaggatgcg cttggcaatg tgtcgcaggg ggacgtgatg
     1741 gcacgcgaca gccacggctc gtacctgcgc agcgaagggc ggctcgtcgc cgcggtcggc
     1801 gttgaaaatc tcgtcgtcat cgagacgagc gacgcgctgc tcgtttccac gcgcgagcgg
     1861 gcggatgacg tcaagctcgc agtcgagcag ctaaagcgcg gcaggcgcac cgagcatgcc
     1921 attcataggc gcgtgtatcg gccgtggggc tactacgagt cgatcgactc cggcgaaagc
     1981 ttccaagtta agcggctcat ggtgaaaccc ggtgcccggc tatcgctgca gaagcacaaa
     2041 aggagagcgg agcactgggt agtggtttcc ggagaagcgt tggtcacgcg aggggaggaa
     2101 gaactgcgcc tgcgcgccaa ccagtcgacg tatatcccca cgggcaccaa gcaccggctc
     2161 gagaacgtcg gggccgatcc gctcttcgtg atcgaagtgc agtccggcga ctacttcggc
     2221 gaagacgaca tcgagcgctt cgccgacgat tatcgacggg cctgagtcag ctgctcgata
     2281 gccgcgacaa cctgcgccac cgccaccctg cggatgcata tggggtcgcc gcattcctcc
     2341 acgctgcgcc agcactggcg cagccagacg agccgccgct cgaacagacg gtcctggtcc
     2401 cggcaggcga cttccgggtc ccatagggcg cggaacgggc tcgttctcca gaagcgtccg
     2461 gggccggtgc tcaccggaga gccgggcccg tagagggcga cggtcggcac gccgaccagc
     2521 cgggcgaggt gagcgatgc
//
LOCUS       DASUNT010000502         2672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_332883, whole genome shotgun sequence.
ACCESSION   DASUNT010000502 DASUNT010000000
VERSION     DASUNT010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332883"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFX94_10110"
     CDS             <1..606
                     /locus_tag="VFX94_10110"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5768576.1"
                     /translation="NPPPAQAREVALRFDELRAAARENRPQLAAQQRMMDRNAKAVEL
                     ARKDYYPDFDVRVSYGQRDNFQDMRREDMISFTVAINLPVWRESKRDPRVAEAEAMRG
                     QASTMYQARLNELDAMLRQQVAAAEQSLKSVRLYETGLLPQARLTADASLAAYRVGRV
                     GFFSLLDSRMTVFNAEVGYAASLAAYNKALAEIEFLVGKSL"
     gene            603..2165
                     /locus_tag="VFX94_10115"
     CDS             603..2165
                     /locus_tag="VFX94_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5768577.1"
                     /translation="MNRTIGIGAAALAAAAALGTGYWLGMRSHSAPEAAPAAGASAAK
                     SERKVLYYRNPMGLPDTSPVPKKDPMGMDYIPVYEGEETPAKSGTVKISLDKVQLLGV
                     RTEPAAERELRRAVQAVGTIQANERLLYKVTSRFEGWIEKLHVNTTGQAVARGQPLME
                     VYSPELVTAQEEYLIALRAIEETRASGSEAQAVMQRMAEGVLRRLHNWGIAESELDML
                     RKEGKARQLLTYRSPAAGVVLQKPSVQGMRFMPGETLYEIADLSSVWLVAEVFERDLG
                     LVKLGQEAKLRILAYPEREFAGKVVFVSPTLEPDTRTARVRIELANPGRRLKPSMYGS
                     LELFAGHPRGKVLAVPDSAVLDSGSRQVVLVQRGEGLFEPRSVKLGMRADGYVEVLDG
                     VAAGDEVVVRANFLIDAESNLKAALETFGGHGGHGAAAKPAASKPAQAHRGQGTVESV
                     DPRAGSIEINHGPIASLKWPAMTMEFKVKDRAMLQGLAKGQAVQFEISEEAAGEFVIE
                     RVAPADHAAHKG"
     gene            2169..>2672
                     /locus_tag="VFX94_10120"
     CDS             2169..>2672
                     /locus_tag="VFX94_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5768578.1"
                     /translation="MLSRIIEWSARNVFVVLLATFFVVAWGVYAVIKTPVDAIPDLSD
                     VQVIIYTEYPGQAPQVVEDQVTYPLTTAMLAVPKSKVVRGLSVFGASFVYIIFEDGTD
                     IYWARSRVLEYLNYVSGRLPRGVTASLGPDATGVGWVFQYAVLAKNRTLEELRSIQDW
                     FVRYQLTK"
BASE COUNT          466 a          849 c          954 g          403 t
ORIGIN      
        1 aacccgccgc cggcgcaggc gcgcgaagta gcgctgcggt tcgacgagct gcgagccgcg
       61 gcgcgcgaga accgcccgca gctcgcggcg cagcagcgca tgatggatcg caacgcgaag
      121 gcggtcgagc tggcgcggaa ggactattac ccggacttcg acgtgcgggt gtcctacggc
      181 cagcgcgaca acttccagga catgcgccgc gaggacatga tcagtttcac cgtggcgatc
      241 aacctgccgg tgtggcgcga gtccaagcgc gatccgcggg tcgccgaggc tgaagcgatg
      301 cgcggccagg cttcgacaat gtaccaggcg cgcctgaacg agcttgacgc catgctgcgc
      361 cagcaagtgg cggccgccga gcagtcgctg aaatcggtgc gtctctacga gaccgggctc
      421 ctgccgcagg cgaggctcac cgccgatgcc tcgctcgcgg cctatcgagt cggtcgcgtc
      481 ggtttcttca gtctcctcga cagccgcatg acggtgttca acgcagaggt cggctatgcc
      541 gccagcctcg ccgcgtacaa caaggccctc gccgagatcg agttcctggt ggggaaatca
      601 ctatgaaccg aacgatagga ataggagcgg cagcactggc cgccgccgca gcgctcggca
      661 cgggctactg gctgggcatg cgcagccact ccgcccccga agccgcaccc gccgcgggcg
      721 cgagcgcagc caagagcgag cgcaaggtcc tctattaccg caatcccatg gggctgcccg
      781 acacctcgcc cgtgccgaaa aaggacccga tggggatgga ttacatcccg gtttacgagg
      841 gcgaagagac cccggccaag tccggcacag tcaagatcag cctcgacaag gtgcagctgc
      901 tcggcgtcag gaccgagccc gccgcggagc gcgagttgcg gcgcgcagtg caggcggtcg
      961 ggaccatcca ggcaaacgag cgcctgctct acaaggtgac gtcgcgcttc gagggctgga
     1021 tcgagaagct gcacgtgaat acgaccgggc aggcggttgc acgcggccag ccgctgatgg
     1081 aggtgtacag ccccgagctc gtcacggcac aggaggagta cctgatcgcg ctgcgcgcga
     1141 tcgaggaaac ccgcgccagc ggctccgagg cgcaagcggt gatgcaacgc atggccgaag
     1201 gggtgctgcg ccgcctgcac aactggggta tcgccgagag cgagctggac atgctgcgca
     1261 aggaaggcaa ggcgcgacag ctgctcacgt accgctcgcc ggctgccggc gtggtgctgc
     1321 aaaagccgag cgtgcagggc atgcgcttca tgccgggcga gacgctgtac gagatcgccg
     1381 atctttcctc ggtctggctg gtcgccgaag tgttcgagcg cgatctcggc ctggtgaagt
     1441 tggggcagga ggcgaagctg cgcatcctcg cctacccgga gcgggaattc gccgggaaag
     1501 tggtgttcgt ttctccgacc ctcgagcccg acacgcgcac cgcccgggtg cgcatcgagc
     1561 ttgcgaaccc cggcaggcgg ttgaagccgt cgatgtacgg gagcctggag ctcttcgccg
     1621 gccatccgcg cggcaaggtc ctggcggtcc cggattcggc ggtgctcgac agcggcagcc
     1681 gtcaggtggt tctcgtgcag cgcggcgaag ggctgttcga gccgcgcagc gtgaagctcg
     1741 ggatgcgcgc cgacggctac gtggaagtgc tcgacggcgt ggccgcgggc gacgaggtcg
     1801 tggtgcgcgc caacttcctg atcgacgcgg aaagcaactt gaaggcggcg ctggaaacct
     1861 tcggcgggca tggcgggcac ggcgccgcgg cgaagccggc ggcatcgaag ccggcgcagg
     1921 cgcaccgcgg ccaaggcacg gtggagtcgg tggacccgcg ggcgggcagc atcgagatca
     1981 accatggccc gatcgcgagc ctgaagtggc cggcgatgac catggagttc aaggtgaagg
     2041 accgggcgat gctacagggc ctggcgaagg gccaggcggt gcaattcgag atctccgagg
     2101 aggcggcggg cgagttcgtg atcgagcgcg tcgcgcctgc cgatcacgcg gcgcacaaag
     2161 gataggccat gctgagccgc atcatcgagt ggtcggcgcg caacgtcttc gtggtgctgc
     2221 tcgccacgtt cttcgtcgtc gcctggggcg tctatgcggt gatcaagacg ccggtggacg
     2281 ccattcccga tctctcggat gtgcaggtca tcatctatac cgagtacccc ggccaggcgc
     2341 cgcaggtggt cgaggaccag gtgacctatc cgctcaccac cgcgatgctc gcggtgccga
     2401 aatcgaaggt ggtgcgcggg ctctcagtgt tcggtgcgtc cttcgtctac atcatcttcg
     2461 aggacggcac cgacatctac tgggcgcgct cgcgggtgct cgagtacctt aactatgttt
     2521 cagggcggct gccgcgcggg gtcaccgcgt cgctcggccc ggacgcgacc ggcgtgggct
     2581 gggtgttcca gtacgcggtg ctcgcgaaga accgcacgct ggaggagctg cgctcgatcc
     2641 aggactggtt cgtgcgctac cagctcacca ag
//
LOCUS       DASUNT010000503         3057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_420174, whole genome shotgun sequence.
ACCESSION   DASUNT010000503 DASUNT010000000
VERSION     DASUNT010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420174"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3057
                     /gene="treY"
                     /locus_tag="VFX94_10125"
     CDS             <1..>3057
                     /gene="treY"
                     /locus_tag="VFX94_10125"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX5768579.1"
                     /translation="PDRGGYVSYPFRELLAILAAESRRARCLVIGEDLGTVAPELRAA
                     LNEAGVLSYRPLLFEKDGSGEYSPPGAYPREALVCVSTHDLPTWRGYWEAHDLNLRQR
                     LGRSPDFKKELASREEEKKKLARALEREGLDLSARSAHAFIARTPCKLALLQPEDVFG
                     MLDQANLPGTIAEHPNWRRKLPLELERWRAHPEVVALREEMAERRVPVAAPLATYRLQ
                     FHKAFRFADATRLVPYLARLGVSHVYASPFLKARPGSTHGYDVIDHNDVNPEIGSRRD
                     LDDFVDALKKHGMGLVVDLVPNHMGVLHADNPWWLDVLENGPRSRYARFFDIDWSPGK
                     LLLPVLGKHYGEALEELKVERAGGRWYVRYFDHRFPLRQKNLQPPKDSLELHRLLETQ
                     HYRLAYWRVASDEINYRRFFEITDLAGLRVEDPDVFTATHGLAARLARRGGIDGLRVD
                     HPDGLADPRQYFERLARLFPRRWIVVEKILAQDEKLPDDWPVHGETGYRFANLLTGVF
                     IDASAEARFDRIYRRFTGEQRSFDEIARESRVLIMGTTLYAELYTLSNRLARIAAGNR
                     YTRDYTASVLRKTLVEIAARFPVYRTYISASGVSGRDREVIDAAVNAAKQATRAADPS
                     VFDFVRSVLLLELAPAGKRREEMLRFAMRFQQFTAPVVAKGIEDTAFYRYTRLIALNE
                     VGGDPSRFGCPLAEFHAAAAQRARLWPSTMLGSSTHDTKRSEDVRARLGVLTELASGW
                     RMALRRWSLMNRAAISRSDEYHFYQALIGIWPGQVSPELTERLKAYLLKAAREAKERT
                     SWINPDAGYEQALERFVVQSLQNEVFVRDVTDLVARVAHLGLLVALSQALVKVASPGV
                     PDYYQGTELWDFSLVDPDNRRPVDYAARMKSLSSLEENPLRLLHDLHDGKAKMHVIRK
                     GLEVRRKFRVLFHGGKYTPLYADGGREENIVAFSLSDGARTLVAIAPRLFTRLMEAHD
                     VAPLGEKVWGNAQISLPGKYVDVMTGRRHEGSALRIAEVLGTFPVAL"
BASE COUNT          512 a         1054 c          998 g          493 t
ORIGIN      
        1 ccccgatcgc ggcggctacg tctcctatcc gttccgcgag ctgctcgcga tcctcgccgc
       61 ggagagccgg cgcgcccgct gcctggtgat cggcgaggac ctcggcacgg tggcgccgga
      121 gctgcgcgcg gcgctcaacg aggcgggcgt tctctcctac cgcccgctgc ttttcgaaaa
      181 agatggctcg ggcgagtact ccccgcccgg cgcgtacccg cgcgaagcgc tggtgtgcgt
      241 aagcacgcac gacctgccga cgtggcgcgg ctactgggaa gcgcacgacc tcaacttgcg
      301 tcaacggctc ggccgcagcc cggatttcaa aaaggaactc gcgtcccggg aagaagagaa
      361 aaagaagctc gccagggccc tcgagcgcga aggcctcgat ctctcggcgc gctcggcgca
      421 cgccttcatc gcgcgcacgc cgtgcaagct cgctttgctg cagcccgagg acgtgttcgg
      481 gatgctcgac caggccaacc tgcccggcac gattgccgag cacccgaact ggcggcgcaa
      541 gcttccgctc gagctcgagc gctggcgtgc gcaccccgaa gtcgttgcgc tgcgcgagga
      601 gatggccgaa aggcgcgtgc cagtggccgc gccgctcgcc acgtatcggc tacagttcca
      661 caaagccttc aggttcgccg atgccacgcg ccttgtaccc tatctcgccc ggctcggcgt
      721 aagccacgtc tacgcctcac cgttcctgaa ggcgcgtccc ggaagcacgc acgggtacga
      781 cgtcatcgac cataacgatg tcaacccgga gatcggtagc cgcagggacc tggatgactt
      841 cgtcgatgcg ctgaaaaagc atggcatggg cctcgtggtc gacctcgtgc cgaaccacat
      901 gggcgtgctg cacgctgaca atccctggtg gctcgatgtg ctggagaacg ggccgcgctc
      961 gcgctatgcg cggttcttcg acatcgactg gtcgccgggc aagctgctgc tacctgtcct
     1021 cggcaagcac tatggcgagg cgctcgaaga gctgaaagtc gagcgtgcgg gcgggaggtg
     1081 gtacgtgcgc tacttcgacc accgctttcc cctcagacag aaaaacctgc agccgcccaa
     1141 ggacagcctc gagcttcacc gcctgctcga gacccagcac taccggctcg cgtactggcg
     1201 cgtcgcctcc gacgagatca attaccggcg cttcttcgag atcaccgacc tggccgggct
     1261 gcgggtcgag gatccggacg tctttacggc gacgcacggg ctcgcggcgc ggctcgcccg
     1321 gcgcggcggc atcgacggct tgcgcgtcga ccaccccgac gggcttgccg atccaaggca
     1381 gtacttcgag cgcctcgcgc ggctcttccc gcggcgctgg atcgtggtgg agaagatcct
     1441 cgcccaggac gaaaagctgc ccgacgactg gccggtgcac ggcgagaccg gctatcggtt
     1501 cgccaacctg ctgacaggcg tcttcatcga cgcctccgcg gaagcgcgct tcgaccgcat
     1561 ctaccggcgc tttaccggcg agcagcgcag cttcgatgag atcgcacggg agagccgcgt
     1621 cctcatcatg gggacgaccc tctatgccga gctttacacg ctctccaacc ggctggcgcg
     1681 catcgcggcc gggaaccgct acacgcggga ctacacggcg agcgtgctgc gcaagacgct
     1741 tgtggagatc gcggcgcggt ttcccgtata ccgcacctat atctcggcga gcggcgtgtc
     1801 cgggcgcgac cgcgaggtca tcgacgcggc ggtgaatgcg gccaagcagg ccacgcgcgc
     1861 ggccgacccg agcgtgttcg acttcgtgcg cagcgtgctc ctcctcgagc tcgcgcccgc
     1921 gggcaagcgc cgcgaggaga tgctgcggtt tgcgatgcga ttccagcagt tcacggcgcc
     1981 ggtggtcgca aagggaatcg aggatacggc gttctaccgc tatacccgcc tcattgcgct
     2041 gaacgaggtc ggtggagacc cctcgcgctt cggttgcccg ctcgccgagt ttcacgccgc
     2101 ggccgcgcag cgcgccaggc tctggccgag caccatgctc ggcagctcca cgcacgacac
     2161 caagcgctcg gaagacgtgc gtgcccgcct gggcgttctg acggagctcg cctccggctg
     2221 gcgcatggcg ctgaggcgct ggagcctgat gaaccgcgcc gccatctcgc gcagcgacga
     2281 gtaccacttc tatcaggcgc tcatcggaat ctggcccggc caggtttcgc ccgagctcac
     2341 cgagcggctc aaggcctact tgctcaaggc cgcgcgcgag gcaaaagagc gcacgagctg
     2401 gatcaacccc gacgccggat acgagcaggc gctcgagcgc ttcgtggtgc agtcgctcca
     2461 gaacgaggtc ttcgtgcgcg atgtgaccga cctggtggcg cgcgttgcgc acctgggact
     2521 gctcgtcgcg ctttcccagg cgctcgtcaa agtggcctcg cccggcgtgc cggactatta
     2581 tcagggcacc gaactctggg atttcagcct cgtcgatccc gacaaccgcc ggccggtgga
     2641 ctacgcggct cgaatgaaat cgctgtcgag cctcgaggag aacccgctcc gcctgctgca
     2701 cgatctgcac gatggaaagg cgaagatgca cgtcatccgc aagggcctcg aggtgaggcg
     2761 caaattccgc gtgctcttcc acggcggaaa gtacacgccg ctctacgccg acggcggccg
     2821 cgaggaaaac atcgtcgcgt tttcgctctc cgatggggcg cgcacgctcg tcgccattgc
     2881 accgcgcctc ttcacgcgcc tgatggaagc gcacgacgtc gcgccgctcg gcgaaaaggt
     2941 gtggggaaat gcgcagattt ccctgccggg gaaatatgtc gacgtgatga ccggccggcg
     3001 gcacgaaggc agcgcgctgc gcatcgccga ggtgctcggc acttttccgg tcgcgct
//
LOCUS       DASUNT010000504         2246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_333151, whole genome shotgun sequence.
ACCESSION   DASUNT010000504 DASUNT010000000
VERSION     DASUNT010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333151"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VFX94_10130"
     CDS             complement(<1..696)
                     /locus_tag="VFX94_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX5768580.1"
                     /translation="MNLPDRPITILIAALGGEGGGVMADWLMEAATQTGYPAQATSIP
                     GVAQRTGATTYYLEIFPVARNALNGKEPVLSLTPSPGNVDVMVASELIEAGRAMLNGF
                     VSPERTALIASTHRIYATVEKMQMADGRFDSDRVLEAAGELAKRAVLFDMRRLAQESG
                     TVINAVLFGAMAGSGVLPLSREACEQAIRRGGRGAEASLRGFAAGYEVAARERPAPAA
                     EPAPRRATDLQEIL"
     gene            complement(693..>2246)
                     /locus_tag="VFX94_10135"
     CDS             complement(693..>2246)
                     /locus_tag="VFX94_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5768581.1"
                     /translation="CHVRGAFKSKKNLAPAVSTRHKLDGPPKEFIYDRLAHPPATFRQ
                     EKLKSDVRLPAARRFISEKGLNERFEGTETQLGIVVQGGLYNSLQRALRILGLADAFG
                     SSRLPILALNVVYPLVPEEIVEFARDKAAVLVLEEGQPEFIEQEIATFLHRADVPTKL
                     HGKDCMHMAGEYNVESLVRGLARFIGLHAPHLDTSPGAAWLSSIQESRDKASSFLGAL
                     PARPPTFCVGCPERPVFSAMKLVAKDIGRPHVSMDVGCHAFASFEPFSQGNTLLGYGM
                     SLAAAAGVAPMQAKRPVAVMGDGGFWHNGLLSGVASNLLNRGDGVLVVMKNGYASATG
                     TQELLSSPPEDARMVAVGQSAAGADRTIENTLKGLGVKWLRTVNNYDVATMAKTYREA
                     MTTPEKGLKVIVAEGECQLERQRRLKPETAKRLSRGERTVRVKYGVDEDTCTGDHSCI
                     RLSGCPTLTVKDNPDPLRRDPVATVIDGCVGCGLCGENAHAAVLCPSFYRAEVVQNPS
                     LWDRLLQRL"
BASE COUNT          338 a          770 c          745 g          393 t
ORIGIN      
        1 taatatctct tgcaggtctg tcgcccgcct gggtgcgggc tcggccgcgg gggcgggacg
       61 ctcgcgtgcc gcaacctcgt agcccgcggc gaagccgcgc aggctcgctt ccgcgccgcg
      121 gccgccgcgg cggatcgcct gctcgcaggc ctcgcgcgag agcggtagca cgccgcttcc
      181 cgccatggcg ccgaacagca ccgcgttgat cacggtgccg ctttcctgcg cgagcctgcg
      241 catgtcgaag agcaccgcgc gcttcgcgag ctcgcccgcg gcctcgagca cccggtccga
      301 gtcgaagcgg ccgtcggcca tctgcatctt ctccacggta gcgtagatgc ggtgggtcga
      361 ggcgatcagc gcggtccgct cggggctcac gaacccgttc agcatcgctc gccccgcctc
      421 gatcagctcg gaagcgacca tcacgtcgac attccccggg ctcggcgtga gcgagagcac
      481 cggctccttc ccgttcaggg cgttcctcgc gaccgggaag atctcgaggt aataggtggt
      541 cgcgcccgtg cgctgcgcga ccccggggat cgaggtggcc tgcgccgggt agccggtctg
      601 cgtggccgct tccatcagcc agtcggccat cacgccgccg ccttcgccgc cgagcgcggc
      661 gatgaggatc gtaatagggc ggtccggaag attcatagcc tttgcagcaa cctgtcccag
      721 agggaaggat tctggacgac ctccgcgcgg tagaacgaag ggcagagcac cgcggcgtgc
      781 gcgttctcac cgcatagccc gcaacccacg cagccgtcga tcacggtcgc gaccgggtcg
      841 cgccgcagcg gatccggatt gtccttcacc gtgagcgtcg ggcagcccga gagccggatg
      901 cacgagtgat cgcccgtgca cgtgtcctcg tccacgccgt acttgactct tacggtacgt
      961 tcgccccggg aaagccgctt tgccgtttct ggtttcaatc ttctctggcg ctcgagctgg
     1021 cattcgccct cggcgacgat cacttttagc cccttctcgg gcgtcgtcat cgcctcgcga
     1081 taggtcttcg ccatcgtggc aacgtcgtag ttgttcactg tgcgcagcca cttcacgccc
     1141 aggcccttca gcgtgttctc gatcgtgcgg tcggcgccgg ccgcgctctg cccgacggcg
     1201 accatgcgcg cgtcctccgg cggcgaggag agcagctcct gcgtgccggt ggccgaggca
     1261 tagccgttct tcatcacgac cagcacgccg tcaccgcggt tgaggaggtt cgacgctacg
     1321 cccgaaagca ggccgttgtg ccagaagccg ccgtcgccca tcaccgcaac cgggcgcttg
     1381 gcctgcatcg gcgcgacgcc cgcggccgcg gcgaggctca tgccgtagcc gaggagcgtg
     1441 ttgccctgcg agaacggctc gaagctcgcg aacgcgtggc agccgacgtc catcgatacg
     1501 tgcggccggc cgatgtcctt cgccaccagc ttcatcgccg agaacaccgg gcgctccggg
     1561 cagccgacgc agaaggtggg cggacgggca ggcagggcgc cgaggaatga cgatgctttg
     1621 tctcttgatt cctgaatgga gcttagccac gcagcaccgg gagaagtgtc caggtgaggg
     1681 gcatgcaggc cgataaatcg cgccagcccg cgcaccagcg actcgacgtt gtactcgccc
     1741 gccatgtgca tgcagtcctt gccgtgcagc ttcgtcggta cgtcggcgcg gtgcaggaag
     1801 gtcgcgatct cctgttcgat gaactcgggt tggccttcct cgagcacgag gacggccgcc
     1861 ttgtctctcg cgaactcgac gatttcttcg ggcaccagcg ggtagacgac gttcagcgcg
     1921 aggatcggca ggcggctcga accgaacgcg tcggccaggc cgagaatgcg cagcgcgcgc
     1981 tgcaggctgt tgtagagccc gccctggacg actatgccaa gctgggtctc agtgccttcg
     2041 aatctctcat tgagtccttt ttctgaaatg aaacggcggg ccgcgggcag gcgcacgtcc
     2101 gacttcagct tctcctgccg gaacgtggcc ggcgggtgcg ccaggcggtc gtagataaat
     2161 tccttcggcg gcccgtccag cttgtggcgc gtggaaaccg cgggcgccag gttcttcttg
     2221 ctcttgaacg cgccgcgcac gtggca
//
LOCUS       DASUNT010000505         3034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_420287, whole genome shotgun sequence.
ACCESSION   DASUNT010000505 DASUNT010000000
VERSION     DASUNT010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420287"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VFX94_10140"
     CDS             <1..593
                     /locus_tag="VFX94_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5768582.1"
                     /translation="VADPVEWAAVFKMPDAGPPGLWRLAFPCDPGLPEDALLEPRSVQ
                     ARMQAFLPLERDYEIRYQSIYRVHQRVAAEWRRGRVLLAGDAAHLNNPLGGFGLNSGI
                     HDAVNLAEKLAKVWLGEADDSLLDHYVRQRRAATVEHVQAMSIRNKRLLEERDPRIHR
                     SRMEELVAIAEDPRRARAHLLESSMIAGLRKAAAIQ"
     gene            678..1121
                     /locus_tag="VFX94_10145"
     CDS             678..1121
                     /locus_tag="VFX94_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768583.1"
                     /translation="MDGSLVALELELDPAPVVPLEGGVLLEPPAALPEGEVSVVPLAE
                     PLAEPLVEPLVVEPPPAESFLAGSDDGDAGLEDGDGELGVVLLPEAEPEAEPDGELGL
                     VVAEPEDAPEPGAARRSPELSPQAVSMPAPSAMEIATAKAESLIL"
     gene            1190..2212
                     /locus_tag="VFX94_10150"
     CDS             1190..2212
                     /locus_tag="VFX94_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX5768584.1"
                     /translation="MDYLLPTTVVGSYPQPGWLIDRAKLGSKVPRVRAPEIWRVPKEL
                     LEQAHDDATVLAIRDMERAGIDIITDGEMRRESYSNRFATELDGIDIRNPGTTINRSG
                     VPIPVPRVAGPIRRRGPVEVRDVVFLKSNTSRRTKITLPGPFTMAQQAQDDYYKDEEA
                     LALAFAAAVNEELRELKEAGADVVQIDEPWLQARPERAARYGVKAINRALEGIRGTTV
                     VHLCFGYAAAVKDKPTGYSFLPQLEDTIASQISIEAAQPKLDLAVLRGLSKTLMVGVI
                     DLGSNDVETPEQVAGRIRAALKHVPPERLVLAPDCGMKYLTREAAFGKLQALAAGAGI
                     VRKELA"
     gene            complement(2255..2569)
                     /locus_tag="VFX94_10155"
     CDS             complement(2255..2569)
                     /locus_tag="VFX94_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768585.1"
                     /translation="MNATTMLTLAAALALAAACDGNRERAENTRPAQAGAAGSSAPSQ
                     PQADTPTTPANLPPPQREAEKRESTSPVQQQVDPKQGEQERDFRSPGEKTGPRGGAKD
                     KD"
     gene            complement(2604..>3034)
                     /locus_tag="VFX94_10160"
     CDS             complement(2604..>3034)
                     /locus_tag="VFX94_10160"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX5768586.1"
                     /translation="CYSAAGKLVQLADCIPSPNGIVVDATVVYVAATRANAVWRLPLA
                     PDGTVVRVGVFLHLSGGRGPDGMAMDESGGLAVAHIDFGAVWIFSRRGEPLYRVQSCR
                     TDMVTNVAYKGDEIYITDSGSGCVLRAKVPTAGKTLYSHS"
BASE COUNT          501 a         1035 c         1022 g          476 t
ORIGIN      
        1 atgtggccga tccggtggaa tgggcggcgg tgttcaagat gcccgatgcc gggccgccgg
       61 ggctctggcg gctcgccttc ccttgcgatc cggggctgcc ggaggacgcg cttctcgagc
      121 cgcgcagcgt ccaggcgcgg atgcaggcct ttctgccgct agagcgcgac tacgagatcc
      181 gctaccagag catctaccgc gtgcaccagc gcgtcgcagc cgaatggcgc cgcggacggg
      241 tgctcctcgc cggcgacgcc gcgcacctca acaacccgct cggcggcttc gggttgaata
      301 gcggcattca cgacgcggtc aacctcgcgg aaaagctcgc caaggtgtgg ctgggcgaag
      361 ccgacgactc gctactcgac cactacgtgc gccagcgccg cgccgccacg gtcgagcatg
      421 tgcaggcgat gtcgattcgc aacaagcggc tgctggaaga gcgcgacccg cgtattcatc
      481 gcagccgcat ggaggagctg gtagcgatcg ccgaggaccc gcgccgcgct cgcgcgcacc
      541 tgctcgagtc ctccatgatc gccgggctgc gcaaggcagc cgcgattcaa tgagccaggc
      601 aaaaaaaggg tcctgacccc cctttttcaa caagagggtc ctgacccttt tcggctcgct
      661 gctactgttt cttctctttg gacggatcgc tcgttgcgct tgagctggag ctcgacccgg
      721 cgcccgtcgt gccgctcgaa ggcggcgtgc tgctggagcc gcccgctgcg ctgcccgagg
      781 gtgaggtatc cgtcgtgccg ctcgcggaac cgcttgccga gccgctcgta gagccgctgg
      841 tcgtggaacc gccgccggcg gaatccttct tggccgggtc ggacgatggg gacgcggggc
      901 tcgaggacgg ggacggcgag ctcggggtag tgctgctgcc cgaggccgaa cccgaagccg
      961 agccggacgg cgagctgggg ctcgtggtgg ccgagcccga ggacgcgccc gagccaggcg
     1021 ctgcccggcg ctcgcccgag ttgtcgccgc aagcggtgag catgccggcg ccgagcgcca
     1081 tggaaattgc tactgccaaa gctgaaagtc tcatactcta gggcctccgt ggttggggta
     1141 aagcatacgg agcaagcttc gggcccgtgg cggtaagatc gaagctcttt tggactatct
     1201 ccttcccacg acagtcgtcg gcagctatcc gcagccggga tggctgatcg accgcgcgaa
     1261 gctcggctcc aaggtgccgc gcgtccgggc gcctgaaata tggcgggtcc cgaaggagct
     1321 cctcgagcag gcgcacgacg acgcgacagt cctcgccatc cgggacatgg agcgcgccgg
     1381 catcgacatc atcaccgacg gcgagatgcg gcgcgaaagc tactccaacc gcttcgcgac
     1441 cgagctcgat ggaatcgaca tccgcaatcc cggtactacg atcaaccgaa gcggcgtgcc
     1501 gatcccggta ccacgcgtcg ccgggccgat acgccgcagg ggaccggtcg aggtgcgcga
     1561 cgtggtgttc ctgaaaagca acacctccag aagaaccaag atcacccttc ccggcccgtt
     1621 caccatggcg cagcaggcgc aggacgacta ctacaaggac gaggaagcgc tcgcgctcgc
     1681 cttcgccgcg gcggtcaacg aggagctgcg cgagctcaag gaagcgggcg ccgacgtcgt
     1741 gcagatcgac gagccgtggc tgcaggcgcg cccggagcgt gccgcgcgct acggcgtgaa
     1801 ggcgatcaac cgtgccctcg aaggaattcg cggaacgacg gtcgtgcacc tgtgcttcgg
     1861 gtacgcggcg gcggtgaagg acaagccgac cggctattcc ttcctgccgc aactcgaaga
     1921 caccatcgca agtcagatct cgatcgaggc cgcccagccg aagctcgacc tcgccgtgct
     1981 gcgcgggctc tccaagacgc tgatggtggg cgtgatcgat cttggatcga acgacgtcga
     2041 gacgcccgag caggtcgccg gccgcatccg cgcggcgctc aagcacgtgc cgcccgagcg
     2101 cctggtgctc gcgcccgact gcgggatgaa atacctgacg cgcgaggccg cgttcggcaa
     2161 gctgcaagcc ctcgccgccg gcgcgggcat cgtgcgcaaa gagctggcct agaccggcga
     2221 acatgggtcc ccgcctgcgc ggggaccacg gacttcagtc cttatcctta gcgccgcccc
     2281 gcggcccggt tttttcgccg gggctgcgga agtcgcgctc ctgctcgccc tgtttgggat
     2341 ccacctgctg ctgaaccggg ctcgtgcttt cgcgcttctc cgcctctctc tgcggcggcg
     2401 gcaggtttgc cggggtagtc ggcgtgtcgg cttgcggctg gctcggcgcg gaagaacctg
     2461 cggcaccagc ctgcgcgggc ctggtgtttt cggcgcgttc tcgattgcca tcgcaggcgg
     2521 cagcgagtgc gagcgcggcg gcgagggtca gcatggtcgt ggcgttcatg acccccgccg
     2581 cgcaacacac gtgcctacac acctcaagaa tgcgagtaaa gcgtctttcc cgcggtgggg
     2641 accttcgccc gcagtacgca gccactcccg gagtcggtga tgtagatctc atcgcccttg
     2701 taggccacgt tggtcaccat gtcggtcctg caactctgca cgcggtacag cggctcgccg
     2761 cgccggctga agatccacac cgcgccgaaa tcgatgtgcg cgaccgcgag cccgcccgac
     2821 tcgtccatcg ccatgccgtc cgggccgcgg ccgcccgaaa ggtgcagaaa aacgcccacg
     2881 cgcaccaccg tgccgtccgg cgcgagcggt agccgccaca cggcattcgc gcgtgtggcc
     2941 gccacgtaaa cgacggtggc atctaccacg atgccgttcg ggctcggaat gcagtcggcc
     3001 agctggacca gcttgccggc cgcgctatag caat
//
LOCUS       DASUNT010000506         2753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_244633, whole genome shotgun sequence.
ACCESSION   DASUNT010000506 DASUNT010000000
VERSION     DASUNT010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244633"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..802
                     /gene="tilS"
                     /locus_tag="VFX94_10165"
     CDS             11..802
                     /gene="tilS"
                     /locus_tag="VFX94_10165"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX5768587.1"
                     /translation="MRGARGMLARAKFSEKTLLRPLLDMTRSEIEAYARRQRLRWIED
                     ESNADESLTRNFIRRRVGPLLEQRFPRWRQSLARAARQFARSDARAHELLREFLRARG
                     LRAPSEAKLVEMLKQLTSGGSRTLIEHDGARLRVYRGQIIEDRRAGTFTSIAWQGESR
                     LAIPELGGELRFRRARGKGIAAGARPLRVRLRAGGERLQPDPRRPRRTLKNLFQEAGV
                     PPWQRDRLPLLFRGEDLVWVPGLGIDARFQASAGQPGVVPAWISK"
     gene            878..1303
                     /gene="ndk"
                     /locus_tag="VFX94_10170"
     CDS             878..1303
                     /gene="ndk"
                     /locus_tag="VFX94_10170"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX5768588.1"
                     /translation="MAVEQTLSVIKPDAVKKNVIGQILARFEGAGLRIVAARMMHLSR
                     AEAEGFYAVHRGRPFFAALVEFMSSGPVLVQVLEGENAIARNRELMGATDPRKAAKGT
                     IRADFAASIDANAVHGSDAPETARNEIAYFFPLSEVYSR"
     gene            1300..2364
                     /gene="rlmN"
                     /locus_tag="VFX94_10175"
     CDS             1300..2364
                     /gene="rlmN"
                     /locus_tag="VFX94_10175"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX5768589.1"
                     /translation="MKQNLLGLDAARLERFLQNEKPFRARQVLRWVHQRGEADFERMT
                     DLARELREKLAAGARVEPPLIVGDTVAADGTRKWLLKVDGANAVEAVFIPETGRGTLC
                     VSSQAGCVLDCAFCSTGKQGFNRNLTTAEIIGQLWLANRLLEGERPVTNVVMMGMGEP
                     LLNLDNVLPALRLMLEDNAYGLSRRRVTVSTAGVVPHMDRLRDECPVALAVSLHAPND
                     ALRDRLVPVNRKYPLVELIAACNRYLEKAPRDFITFEYVMLEGVNDAADQARELVALA
                     ARVRCKFNLIPCNPLSNSGFKRSSPERIRRFSEVLQRAGLTVTTRKTRGDDIAAACGQ
                     LAGNVADRTRRKLVLKEARV"
     gene            2361..>2753
                     /locus_tag="VFX94_10180"
     CDS             2361..>2753
                     /locus_tag="VFX94_10180"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5768590.1"
                     /translation="MNGRTAILLAMLAGCAAQTPDPVHDTATLPGEAGERRNRARLHT
                     ELASLYYTNGNMAVALDELRAAAAADSSYGPAHGMYGVVYMRLNEPARAGESFERALR
                     LAPNDPDINHNYGWFLCQTGREEASIKHF"
BASE COUNT          466 a          896 c          948 g          443 t
ORIGIN      
        1 cggcgctggg ctgcgaggtg cacgcggcat gcttgcaaga gccaaattca gtgagaagac
       61 tctactgcgt ccgttgctcg acatgacacg cagtgagatc gaagcgtatg cgcgccgcca
      121 acggctcaga tggatcgagg acgagagcaa cgcagacgag tcgctgacgc gcaactttat
      181 ccggcggcgg gtcggccctc tgctcgagca gagatttccc cgatggcggc agagcctcgc
      241 gcgggcggcg cggcagttcg cgcgcagcga tgcccgggcg cacgagctgc tgcgcgaatt
      301 tctccgtgca agggggctgc gcgctccgag cgaagcgaag ctcgtcgaga tgctgaagca
      361 gctcacctcc ggcggcagcc ggacgctgat cgagcatgac ggcgcgcgcc tgcgggttta
      421 ccggggccaa atcatcgaag accggcgcgc cggcacgttc acgtcgattg catggcaagg
      481 cgagagcagg ctcgcgatcc ccgagctcgg cggcgagctg cggttccggc gcgcgcgcgg
      541 caagggcatc gcggcaggtg ccaggccgct gcgcgtgcgg ctgcgcgcgg gtggcgagcg
      601 cctccagccc gacccgcgcc ggccgcggcg cacgctgaag aaccttttcc aggaagcggg
      661 cgtgccgccg tggcagcgcg accgcctgcc gctgctcttc cgcggcgaag atctggtctg
      721 ggtacctggc ctggggatcg atgcgcgctt tcaggcgtcg gcagggcagc ccggcgtcgt
      781 gccggcatgg atctccaagt gacgcgcagc atcggaattg cgggaatcgc gtaaaatgac
      841 cggttaattt cttgatttag caaggagaat gcagtcaatg gcggtggagc aaacgctctc
      901 ggtcatcaag ccggacgcgg tgaaaaagaa cgtgatcggg cagatcctcg cgcgcttcga
      961 gggcgcgggc ttgcgtatcg tggcggcgcg catgatgcat ctttcgcgcg ccgaggcgga
     1021 gggtttctac gccgtgcacc gcggacggcc gttcttcgcc gcgctggtgg agttcatgag
     1081 ttcggggccg gtactggtac aggtgctcga aggcgagaac gccattgcgc gcaatcgcga
     1141 gctgatgggc gcgaccgatc cgcggaaggc ggccaaaggg acaattcgcg ccgatttcgc
     1201 cgcgtccatc gatgcgaacg cggtgcacgg ctcggacgcg ccggaaaccg cgcgcaacga
     1261 aatcgcctac ttcttcccgc tttccgaggt ttactcgcgg tgaagcagaa tctcctcggt
     1321 ctggacgctg ctcgcctgga acggtttctt cagaatgaaa aaccattccg ggcgcgccag
     1381 gtcctgcgct gggtgcacca gcgcggcgaa gccgacttcg agcgcatgac cgacctcgcg
     1441 cgcgagctgc gcgagaagct ggcagccggc gcgcgcgtcg agcctccgct catcgtcggc
     1501 gatacggtgg ccgcggacgg cacgcgcaaa tggctgctca aggtggacgg cgccaacgcg
     1561 gtcgaggcgg tattcattcc cgagacgggg cgcggcaccc tttgcgtttc gagccaggcg
     1621 ggctgcgtgc tcgactgcgc cttctgctcg accggcaagc agggattcaa tcgcaacctg
     1681 acgacggcgg agatcatcgg ccagctgtgg ctcgccaacc ggctgctgga aggggagaga
     1741 cccgttacga acgtcgtaat gatgggcatg ggcgagccgc tgctcaacct cgacaacgtc
     1801 ctcccggcgc tgcgcctcat gctggaggac aatgcctacg gactttcccg ccgacgggtc
     1861 accgtgtcca cggccggtgt ggttccccac atggaccgcc ttcgcgacga atgcccggtg
     1921 gcgcttgccg tatctctgca cgctccgaac gacgctctgc gcgaccggct ggtgccggtg
     1981 aaccgcaagt acccgctggt cgagctgatc gccgcgtgca accgctatct tgagaaggcg
     2041 ccgcgcgact tcatcacctt cgagtacgta atgctcgaag gcgtgaacga cgcggccgac
     2101 caggcgcgcg agctggtcgc gcttgcggcc agggtacgct gcaagttcaa cctcatcccg
     2161 tgtaatccgt tgtccaattc cggcttcaag cgctcctcgc ccgagcgcat ccggcgcttc
     2221 agcgaggtgc tgcagcgcgc cgggctcacc gtgacgacgc gcaagacccg cggcgacgac
     2281 atcgccgccg cgtgcggaca gctcgcaggc aacgtggccg accgcacgcg ccgcaagctc
     2341 gtgctgaagg aggcccgggt atgaacggcc gcacagccat cctgctcgcg atgctcgcgg
     2401 gctgcgctgc gcagaccccc gatcctgtgc acgataccgc caccctgccg ggcgaggcgg
     2461 gcgagcggcg caaccgcgcg cgtctgcata ccgagctcgc ttctctttat tacaccaacg
     2521 gcaacatggc ggtggcgctg gacgagctgc gcgcggctgc ggcggccgac tccagttatg
     2581 gcccggcgca cggcatgtac ggggtcgtgt atatgcgcct caacgagccc gcgcgggcgg
     2641 gggagagctt cgagcgcgcc ctgcgccttg cgccgaacga tcctgatatc aaccacaact
     2701 atggctggtt tctgtgccag acgggccgcg aggaggcgtc gatcaagcat ttc
//
LOCUS       DASUNT010000507         2859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_857801, whole genome shotgun sequence.
ACCESSION   DASUNT010000507 DASUNT010000000
VERSION     DASUNT010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857801"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..343
                     /locus_tag="VFX94_10185"
     CDS             98..343
                     /locus_tag="VFX94_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768591.1"
                     /translation="MLRSCWQRRHSRSTADRLPERLERGGDDRHDGARAKAATRLEIG
                     DQHIGVPWKDVRIGEKVQYVRVPLREVRSGTSRAPGA"
     gene            340..630
                     /locus_tag="VFX94_10190"
     CDS             340..630
                     /locus_tag="VFX94_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768592.1"
                     /translation="MNELIGDYVSLKDVPRYGIVTDVIFSDQGKARAVVVHRGAADFG
                     APGPFAYPYPHGGFDPRAYDYPLPYSSAEIGRFGRFDYVKLGELSRFASRAK"
     gene            complement(602..>2859)
                     /locus_tag="VFX94_10195"
     CDS             complement(602..>2859)
                     /locus_tag="VFX94_10195"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEX5768593.1"
                     /translation="QSLVVENGTLRYAPRDFALEALTLHARELSTLDAAAGTYEAAAR
                     LADGAGQFAARGAVALAPFAAEGTLSVAALRAAQLLPEAAGQLQGEAMYTYRDGEFTF
                     HDVSIAGSGLAYAGIELPQATLRAGKVPIPPRQPFQAAGEASVAPHGKIAAQGRVGLS
                     PLELHLNLTAEALPLAQAQRWLPQGLGVDIASGSVSGTGRLLFENAISYQGSAAIRDL
                     RLEERGSEALLLGWKAGETQSLRFTSSPLAIEADEIALRAPEGRLVIEENGGVNFAAV
                     FPHSEGTGGKGLQASFERLRIEEGVLHFADRSLATPFQATIRELSGTVLGFSTEKNDP
                     ARVRLNGRVQPYGVARIRGTINLNAPTTLADITAVFRNLPLEAFNPYVAKFAGYRIES
                     GRLSAELRYEVRDGRLSGTNQLAFQNMQLGEKLEQKGLLDLPFELAVALLADSKGRID
                     LTIPVRGNLNDPQFDMGAIVSRALGNVVRKVVTAPFRALAALFGGKDGDEHPGEIGFA
                     PGSAVLSPPAEEDVARVAQALGERPRLGVEVQGAYDAQRDLQALRMRAAREAVASAAG
                     AELPLDLSNPKVRETAEKLHLSRGGERQVLDALRKSEADYGRLLLERLAAMAPVDENA
                     AQELARERAESVHAALLEHGVDPARVGFGAISGEKQPAATLARKPAEEVSAAAGAGGA
                     SSSYVEDPVRAAQERLNAAGFDAGPVDGILGPRTKRALIHYQAVSGLELTGELDRATR
                     ERLISPDSRSG"
BASE COUNT          421 a          998 c         1009 g          431 t
ORIGIN      
        1 tctgacggtt ttgcagagcg cccgctgtag tccttgcgaa cgttgcgccc gcgttacagc
       61 gggcgctgat ggaggatgcc tatgagattc caaacccttg ctgcgctcct gctggcagcg
      121 ccggcattcg cgcagcacag cagatcgcct accagaacgc ctggagcgcg gaggagatga
      181 tcggcacgac ggtgcgcggg cgaaagcggc gacgagactc gagatcggcg accagcacat
      241 cggcgtgccg tggaaggacg tgcgcatcgg cgagaaggtg cagtacgtgc gggtgccgtt
      301 gcgcgaagtg cgttcgggca cctcgagagc tcctggcgcg tgaacgagct gatcggcgat
      361 tacgtgagcc tgaaggacgt gccgcgctat ggcatcgtga ccgacgtgat cttcagcgac
      421 caaggcaagg cgcgggcggt cgtcgtgcac aggggcgccg ccgatttcgg cgcgccgggg
      481 ccgttcgcct acccctaccc gcacggcggc ttcgacccgc gcgcctacga ctatccgctg
      541 ccctactcga gcgcggagat cgggcgcttc ggccgctttg actacgtaaa gctcggcgag
      601 ctcagccgct tcgcgagtcg ggcgaaataa gccgctcgcg cgtcgcgcgg tcgagctcgc
      661 ccgtgagctc gagcccgctt acggcctgat agtgtatgag cgcgcgcttg gtgcgcgggc
      721 cgaggatgcc gtcgaccggg ccggcgtcga agcccgcggc attcagcctc tcctgagcgg
      781 cgcgcaccgg gtcttcgacg taagaagaac tggcgccgcc ggcgccggcg gcggcgctca
      841 cctcttccgc cggcttacgt gcaagcgtgg ccgcaggctg cttctcgccg gaaatcgcgc
      901 cgaaccctac gcgcgccggg tcgacgccgt gctcgaggag cgcggcgtgc acgctttcgg
      961 cgcgctcgcg cgcgagctcc tgcgcggcgt tctcatcgac cggcgccatg gcggcgagtc
     1021 gctcgaggag aagccggccg taatcggcct cgctcttgcg gagcgcgtcg agcacctgac
     1081 gctcgccgcc gcgcgaaagg tggagcttct cggccgtctc gcgcaccttc ggattggaaa
     1141 gatcaagagg caactctgcg cccgccgcgc tcgccacggc ctcacgcgcg gcgcgcatgc
     1201 gcagcgcctg caagtcgcgt tgggcatcgt aggcgccctg cacttcgacg ccgagccgcg
     1261 gacgctcgcc gagcgcctgc gccacgcgcg ccacatcctc ctccgccggc ggcgacagca
     1321 ccgccgagcc gggcgcgaag ccgatctcgc ccgggtgctc gtcgccgtcc tttccgccga
     1381 agagcgcggc gagcgcgcgg aagggcgcgg tgacgacctt ccgcaccacg ttgccgagcg
     1441 cgcgcgagac gatcgcgccc atgtcgaact gcgggtcgtt caggttgccg cgcaccggga
     1501 tcgtgagatc gatgcggccc ttcgagtcgg cgagcagcgc gaccgccagc tcaaacggga
     1561 ggtcgagcag gcccttctgc tcgagcttct cgccgagctg catgttctgg aaggcgagct
     1621 ggttggtgcc ggagagcctt ccgtcgcgca cctcgtagcg cagctcggcc gacaggcgcc
     1681 cggactcgat gcggtagccg gcgaacttcg ccacgtacgg attgaacgct tcgagcggca
     1741 gattgcggaa caccgccgtg atgtcggcga gcgtggtggg cgcgttcagg ttgatcgtgc
     1801 cgcggatgcg cgccacgccg tagggctgca cgcgcccgtt caggcgcacg cgcgccgggt
     1861 cgttcttctc ggtgctgaag ccgagcaccg tgccggagag ctcgcggatc gtggcctgga
     1921 acggcgtggc gagcgagcgg tcggcgaagt gcagcacgcc ctcctcgatg cgcagacgct
     1981 cgaagcttgc ttgcaggccc ttgccgccag tgccttcgct gtggggaaat accgcggcga
     2041 aattcacccc accgttctcc tcgatgacga gccgcccctc gggcgcgcgc aacgcgatct
     2101 cgtccgcctc gatcgcaagt ggcgaagacg tgaaccgaag cgattgcgtt tccccggctt
     2161 tccagccgag gagcagcgcc tcgctgccgc gctcctccag gcgcaggtcg cggatcgcgg
     2221 cactgccctg gtaggagatc gcgttttcga acagcagccg cccagtgccg gatacgctgc
     2281 cagatgcgat gtcgaccccg agcccttgcg gaagccagcg ctgcgcctgc gcgagcggca
     2341 atgcctcggc agtgaggttg agatgcagct cgagcggcga gagaccgacg cgcccctgcg
     2401 ccgcgatctt cccgtgcggc gccaccgagg cctcgcccgc cgcctggaat ggctggcgcg
     2461 gcgggatcgg cactttcccg gcgcgcagcg tcgcctgcgg cagctcgatg ccagcatagg
     2521 cgagcccgct gcccgcgatc gacacgtcat gaaacgtgaa ctcgccgtcg cggtaggtgt
     2581 acatcgcctc gccttgtagc tgaccggcgg cctcgggcag gagctgcgcc gcgcgcagcg
     2641 ccgcgacgct gagcgtgcct tcggccgcga acggcgcgag ggcgaccgcg cctcgcgcag
     2701 cgaactgtcc cgcgccgtcg gcgagacgcg ccgcggcctc gtacgtgccc gcggccgcgt
     2761 cgagcgtcga gagctcgcgc gcgtgcagcg tcagcgcctc gagcgcaaaa tcgcgcggcg
     2821 cgtagcgcag cgtgccgttc tcgacgacga gactctgga
//
LOCUS       DASUNT010000508         2967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_594209, whole genome shotgun sequence.
ACCESSION   DASUNT010000508 DASUNT010000000
VERSION     DASUNT010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594209"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VFX94_10200"
     CDS             <1..365
                     /locus_tag="VFX94_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768594.1"
                     /translation="QAQATDGWVGRIPTAGQMLLGFILPFALAFIAIPLESLIHSSRT
                     VGGVLLAALVSTLALALRVLGNAARKMSKVLVTLYDVAIVIPLTVEHLAKSGMSMRRP
                     AADPDDSAVHPVRARGSR"
     gene            362..1021
                     /locus_tag="VFX94_10205"
     CDS             362..1021
                     /locus_tag="VFX94_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5768595.1"
                     /translation="MIKRLLLLGLALFAAGPAGCGDPRSHAQAVYVLIDTSGTYAQEA
                     AKAQLIVNYLLGTLQPGDSLAVARVESRSFTEKDIIAKATFDQRPSQANAQKRAFREA
                     TDNFLRTVKSSKHTDITGGLLQGAEFLNETGAGTKTIVVFSDMQEELDKITVRNIPMN
                     LKRIRVIAVNVVKLNTDNIDPRRYFGRLEAWERRVVSAGAAEWRVINDLERLDAIFSR
                     S"
     gene            1095..2105
                     /locus_tag="VFX94_10210"
     CDS             1095..2105
                     /locus_tag="VFX94_10210"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5768596.1"
                     /translation="MRYPNPTRRRIVAGAGAALALGPLNAHAQPYPSKNIRVVIPTGQ
                     GGGADRLARTFDDFWGPLLKTNFEYGFFPGAAGQVGYELYISKRERDGYNLLFGNMGP
                     EMIMYALQKPPYRFPEDYQYFCRLDVDDSIVFVRAESRIKRIEDLIAEGKKRAVNVAV
                     SRLPHPASIGLLALGAATKSRFNLVPYGGGNPTTVAVMNGEADCGALPIAGVAALGDK
                     MRILGIFNDEAEKMAKYSGNAPSVNKVFGTKIPSLYSSRAWAIHTEVIEKFPDRFALL
                     EKTARQVFDNPKYREEYAKTGAPIETVQYGDRKLCTEYAKGMVELTNEYRSLLSAKDK
                     KG"
     gene            2113..2580
                     /locus_tag="VFX94_10215"
     CDS             2113..2580
                     /locus_tag="VFX94_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768597.1"
                     /translation="MKSPRGADLVIPALALGFAAYFFYSIVDLAWEAKANGVVIGGVL
                     VLLIAIQAVRTALEVARGRANLRLDPVVSPRDVLGKRLGLVAITIAFIATLHWLGLTL
                     GLFIAMVAALWVMGARGKVLALLPLAVAAAAYLLFVALLKSDIPHGPIERLLS"
     gene            2577..>2967
                     /locus_tag="VFX94_10220"
     CDS             2577..>2967
                     /locus_tag="VFX94_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5768598.1"
                     /translation="MSWDFLLAQFAAQFGLWWVVLPGIRLGIVVGILPGFSPQNTLIM
                     LLPLTLAVPVDQAMAFMISLYCANHLAGGIPAILVKIPGSGGAAATTLDGYPMTQKGK
                     AQQALVLCFTASVFGGLITTLATIALLP"
BASE COUNT          494 a          979 c          980 g          514 t
ORIGIN      
        1 agcaggcgca ggcgaccgac ggctgggtcg gccggattcc gaccgcggga cagatgctgc
       61 tcggcttcat tctgccgttc gcgctcgcat tcatcgccat tccgctcgag tcgctcatcc
      121 attcgtcgcg taccgtcggc ggcgtgctgc tcgccgcgct ggtgtcgacg ctcgcccttg
      181 cgctgcgggt gctcggcaac gcggcgcgca agatgtccaa ggtactcgtc acgctctacg
      241 acgtagcgat cgtgatcccg ctaacggtgg agcatttggc gaagtcgggc atgtcgatgc
      301 gccgacccgc ggcggacccg gacgactccg cggttcaccc cgtgagagcg aggggcagcc
      361 gatgatcaag cgactgctgc tgctcggcct cgcgttattc gcggcggggc ccgccggttg
      421 cggcgatccg cgcagccatg cccaggcggt gtacgtgctg atcgatacgt cgggcaccta
      481 cgcccaggag gcggccaagg cgcagctcat cgtcaactac ctgctcggga ccctgcagcc
      541 cggcgactcg ctcgcggtgg cgcgcgtcga aagccgcagc ttcactgaaa aggacatcat
      601 cgcgaaagcg accttcgacc agcggccctc gcaggccaac gcgcaaaagc gcgcgttccg
      661 cgaggcaacc gacaatttcc tgaggaccgt gaagtcgagc aagcataccg acatcaccgg
      721 cgggctgctg cagggcgcgg aattcctgaa cgagaccggt gccggcacca agaccatcgt
      781 ggtgttctcc gacatgcagg aagagctcga caagatcacc gtgcgcaaca tcccgatgaa
      841 cctgaagcgc atccgggtga tcgccgtgaa cgtggtcaag ctgaacaccg acaacatcga
      901 cccgcggcgc tatttcgggc ggctcgaggc gtgggagcgg cgcgtggtga gcgccggggc
      961 ggccgagtgg cgcgtcatca acgacctgga gcggctggac gccatctttt ccaggagcta
     1021 gttcaaactg cggttcctcc ccgggggcgg gtcattctaa tatccgtacc cctgactacc
     1081 tggaggagga gtccatgagg tatcccaatc cgacgcgccg gcgcatcgtg gccggcgccg
     1141 gcgccgcgct tgcgctcggc cccctgaacg cgcatgcgca gccctatcca tccaagaaca
     1201 tccgcgtcgt cattccgacc gggcagggcg gcggcgccga tcgcctggcg cgcacgttcg
     1261 acgacttctg ggggccgctg ctcaagacga acttcgagta cggcttcttc cccggcgcgg
     1321 ccgggcaggt cgggtacgag ctctacatca gcaagcgcga gcgcgacggc tacaacctgc
     1381 ttttcggcaa catggggccg gagatgatca tgtacgcgct gcagaagccg ccgtaccgct
     1441 ttcccgagga ctatcagtac ttctgccgtc tcgacgtcga cgacagcatc gtgttcgtga
     1501 gagccgaaag ccgcatcaag cgcatcgagg acctgatcgc cgagggcaag aaacgcgcgg
     1561 tcaacgtggc ggtgagccgc ctgccgcacc ccgcctcgat cggcctgctt gccctgggcg
     1621 cggccaccaa gtcgcgcttc aacctcgtgc cctatggcgg cggcaatccg accaccgtgg
     1681 cggtgatgaa cggcgaggcc gactgcggcg cgctgccgat cgccggcgtc gcggcgctcg
     1741 gcgacaagat gcgcatcctc ggcatcttca acgacgaggc cgagaagatg gccaagtaca
     1801 gcggcaacgc gccatcggtc aacaaggtgt tcggcaccaa gatcccgtcg ctttactcct
     1861 cgcgcgcctg ggcgatccac accgaggtga tcgagaagtt tcccgaccgc tttgcgctgc
     1921 tcgagaagac cgcgcggcag gtgttcgaca atccgaaata ccgcgaggag tatgcgaaga
     1981 ccggcgcgcc gatcgagacc gtccagtacg gcgaccgcaa gctgtgcacc gagtacgcca
     2041 agggaatggt ggagctcacc aacgagtatc gctccctcct gagcgccaaa gacaagaaag
     2101 gctagacgcc gcgtgaagtc tccgcgcggt gcggacctgg tgatcccggc gctcgcgctc
     2161 gggttcgcgg cctacttctt ctactcgatc gtcgatctcg cctgggaggc aaaggcgaac
     2221 ggcgtggtga tcggtggcgt gctcgtgctg ttgatcgcca tccaggcggt gcgcaccgcc
     2281 ctggaagtgg cgcgggggag ggcgaatctg cggctcgacc ccgtggtgtc gccgcgcgat
     2341 gtgctcggca agcgtctcgg cctggtggcg atcacgatcg cgttcatcgc gacgctgcac
     2401 tggctggggc tcacgctcgg attgttcatc gccatggtgg cggcgctgtg ggtgatgggc
     2461 gcgcgcggca aggtgcttgc cctcctgccg ctcgcggtag ccgccgccgc gtatctgttg
     2521 ttcgtcgcgc tgctcaagtc cgacattccg cacgggccga tcgagaggct cctttcttga
     2581 gttgggattt cctgctcgcg caatttgcgg cgcagttcgg cttgtggtgg gtcgtgctgc
     2641 ccgggatccg gctcggtatc gtggtcggga tcctgccggg attcagcccg cagaacacgc
     2701 tgatcatgct gctgccgctc acgctcgcag tgccggtcga ccaggccatg gcgttcatga
     2761 tctcgcttta ctgcgccaat cacctggccg gcggaattcc ggccatcctg gtgaagatcc
     2821 cgggttccgg cggcgcggct gcgaccacgc tcgacggcta cccgatgacg cagaagggca
     2881 aggcgcagca ggcgctggtg ctttgcttca ccgcctcggt gttcggcggg ctcattacca
     2941 cgctcgcgac gatagcgctg ctgccct
//
LOCUS       DASUNT010000509         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_333637, whole genome shotgun sequence.
ACCESSION   DASUNT010000509 DASUNT010000000
VERSION     DASUNT010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333637"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VFX94_10225"
     CDS             <1..558
                     /locus_tag="VFX94_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEX5768599.1"
                     /translation="QLPLPRHIDTARVLESIAPAKAVDGFHEANLGALLAGRPGVVPC
                     PPLGCMRLIEHAGVQIAGRHAVVVGRSNIVGKPVAHLLLQKDATVTVCHSKSSDLSQI
                     ARQADILVAAVGRAKLVTAAMVKPGACVIDVGVNRLPDGKLAGDVDFESVSHVAGWIT
                     PVPGGVGPMTIAMLLENCLRAASRN"
     gene            539..2572
                     /locus_tag="VFX94_10230"
     CDS             539..2572
                     /locus_tag="VFX94_10230"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX5768600.1"
                     /translation="MLPQETNPLLDFSGLPRFAEIRPEHVTPAIELLLSDARSTVKAS
                     ENAAAEWDAFVAPLEDANERLGRAWGQVAHLHAVMDSPELREVYNANLPKITKYWTEL
                     GQNLHLFEKYKALKSSPEFEGLTAARRRIIENAIRDFRLGGAELAPEKKKRYAEIQEE
                     LARLSAKFSENLLDATNAFSVYVTKAQTSGIPEDVLEAAHEAAQKDGKDGWKLTLHMP
                     SYLPVMQYADERSLREKMYYASSTRASEFGNSQWDNAPLIAKIVPLRRELAQLLGYAN
                     FAEVSLVPKMAQSASQVLAFLDDLARRARPFAERDVAELKEFSRTELGIAELQAWDVA
                     YASEKLRLKRYSFSDQEVKQYFPEDVVVRGLFRVAETLYQVQIRQSNAAGWHPDVRFF
                     EVRDANGTAIGQFYMDLYARETKRGGAWMDDAISRRRKGDRIQIPVAYLNCNFSRPVG
                     GKPALFTHDEVLTLFHEFGHGLHHLLTRVEDLGVSGISGVEWDAVELPSQFMENFCWE
                     WDVLCEMTRHVDTGLPIPRALFDRMLAAKNFQSGLAMLRQIEFAVFDMRLHSDFDPAS
                     GRTALELLEEVRRQVAVLFPPKYNRFPNSFSHIFAGGYAAGYYSYKWAEVLSADAYSL
                     FEEKGVLSPEAGGRFRDEILAVGGSRPAAESFKAFRGREPNVDALLRHNGMTV"
BASE COUNT          461 a          850 c          856 g          441 t
ORIGIN      
        1 cagctcccgc tgcccaggca catcgacacc gcccgcgtgc tcgaatccat cgcgcccgcc
       61 aaggccgtcg acggcttcca cgaagccaac ctcggcgcgc tcctcgccgg gcgtccgggc
      121 gtcgtgccgt gtccgccgct cggctgcatg cggctgatcg agcacgcggg cgttcagatc
      181 gccggccggc acgcggtggt ggtgggccgc tccaacatcg tcggcaagcc ggtggcgcat
      241 ctgctcttgc aaaaggacgc gacggtgacg gtatgccatt cgaagtcaag cgatctttcg
      301 cagatcgcgc gacaggccga catcctggtc gcggccgtgg gccgggcgaa actcgtgacg
      361 gcggcgatgg tcaaaccggg agcctgcgtg atcgacgtcg gcgtgaaccg cctgcccgac
      421 ggcaagcttg caggcgacgt ggatttcgaa tccgtcagtc acgttgcggg ctggatcacg
      481 ccggtgcccg gcggcgtggg cccgatgacg atcgcaatgc tcctggagaa ctgcctgcgt
      541 gctgcctcaa gaaactaacc cgctgctcga cttctcgggc ctgccgcgct ttgcggagat
      601 aaggcccgag cacgtcacgc ccgcgatcga gctcctgctc tcggacgcgc gctcgaccgt
      661 gaaagcctcg gagaacgccg cggccgagtg ggacgcgttt gtcgcgccgc tcgaggacgc
      721 gaacgagcgc ctcggccgcg cctggggcca ggtcgcgcac ctgcatgccg tgatggacag
      781 ccccgagctg cgcgaggtct acaacgccaa cctgccgaag attaccaagt actggaccga
      841 gctcggccag aacctgcatc tcttcgagaa gtacaaggcg ctgaaaagct cgccggaatt
      901 cgaaggactc actgccgcga gaaggcgcat cattgagaac gcgatccgcg atttccgcct
      961 cggcggcgcc gagctcgcgc cggagaagaa aaagcgctac gccgagatcc aggaggagct
     1021 cgcgcggctc tcggcgaagt tctccgagaa cctgctcgac gccacgaacg ctttttccgt
     1081 ctacgtcacg aaggcgcaga cctcgggcat ccccgaggat gtgctcgaag ccgcgcacga
     1141 agcggcgcag aaggacggca aggacgggtg gaagctcacg ctccacatgc cctcctacct
     1201 gccggtgatg caatacgccg atgagcgctc gctgcgcgag aagatgtact acgcgagcag
     1261 cacgcgcgcc tcggagttcg gcaactcgca gtgggacaac gcacccctca tcgcgaagat
     1321 cgttcccctg cgccgcgagc tcgcgcagct cctcggttac gcaaacttcg cggaagtctc
     1381 gctcgtgccg aagatggcgc agtccgcgag ccaggtgctc gccttcctcg acgacctcgc
     1441 gcgccgcgcg cggcctttcg cagagcgcga tgtcgcagag ctgaaggagt tctcgagaac
     1501 tgaacttggt attgccgagc tgcaggcctg ggatgtggcc tacgcttccg agaagcttcg
     1561 cctcaagcgc tactcgttct ccgaccagga agtgaagcag tatttccccg aggacgtggt
     1621 cgtgcgcggt ctcttccgcg tagcggagac tctttaccag gtgcagattc ggcagagcaa
     1681 tgctgcgggc tggcatcccg acgtgcgctt cttcgaagtg cgcgatgcga acgggaccgc
     1741 gattggccag ttctacatgg acctttatgc gcgcgagacc aagcgcggcg gcgcatggat
     1801 ggacgacgcg atctcgcgcc ggcgcaaggg cgaccgcatc cagatcccgg tggcgtatct
     1861 caattgcaac ttctcgcgcc cggtaggggg aaaaccggcg cttttcacgc acgacgaggt
     1921 gctcacgctg ttccacgagt tcgggcacgg gctgcaccat ctccttaccc gcgtcgagga
     1981 tctcggcgta tcgggcatca gcggcgtgga gtgggacgcg gtcgagctgc cgagccagtt
     2041 catggagaac ttctgctggg agtgggacgt gctgtgcgag atgacgcggc acgtggatac
     2101 gggcttgccc attccgcgag cgctctttga ccgcatgctc gccgcgaaga acttccagag
     2161 cggcctcgcg atgctgcgcc agatcgagtt cgcggtgttc gacatgcggc tgcattcgga
     2221 tttcgacccg gcgagcggac gcaccgcgct cgagctcctg gaggaagtgc gcaggcaggt
     2281 cgcggtcctc ttcccgccga agtacaaccg cttcccgaac agcttctcgc acatcttcgc
     2341 cggcggctat gcggcgggct actacagcta caagtgggcc gaggtgctct ccgcggatgc
     2401 ttacagtctg ttcgaggaaa aaggcgttct cagtcccgaa gcgggcgggc gtttccgcga
     2461 cgagatcctt gccgtcggcg gcagccggcc ggcggcggaa tcgttcaagg cattccgcgg
     2521 ccgcgagccg aatgtcgacg cgttgctgcg ccataacggc atgacggtct gaagctcgcg
     2581 acctggaacg tcaactcgtt gcgggtgc
//
LOCUS       DASUNT010000510         3934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1121085, whole genome shotgun sequence.
ACCESSION   DASUNT010000510 DASUNT010000000
VERSION     DASUNT010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121085"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /gene="ssb"
                     /locus_tag="VFX94_10235"
     CDS             <1..349
                     /gene="ssb"
                     /locus_tag="VFX94_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX5768601.1"
                     /translation="KDKQSGELKEATEWHRIAFFGRLATIAGEYLRKGSQVYVEGSLR
                     TRKWQDKDGQDRYSTEIRGDVMQMLGRREGGGEPRVEAAAESKSGAARSPAPAAAKKP
                     AGKFDDLEDDIPF"
     gene            372..677
                     /locus_tag="VFX94_10240"
     CDS             372..677
                     /locus_tag="VFX94_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX5768602.1"
                     /translation="MPIYPYRCSACGQEHDVLQKVSEPPLSECPSCGKPGMQKLLTAA
                     GFQLKGSGWYATDFKGGAKKPEDKKQPAEPKPADSKSDSKTDTKTSPKSETKTEAAA"
     gene            737..1324
                     /locus_tag="VFX94_10245"
     CDS             737..1324
                     /locus_tag="VFX94_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX5768603.1"
                     /translation="MKKYLITGLLIWIPLVITLWVLKLVVDVLDQSLLLLPEAWQTKH
                     WLGVHVPGLGAILTVFIVFLTGLFATNFFGAQLVELWHEILHRIPVVNSIYSSVKQIS
                     DTLFSSSGQAFRKALLVQWPQEGMWTIAFLTGAPGGGVAEHLPPDCVAVYVPTTPNPT
                     GGYFVIVARKNVVELDMSVDQALKYIISMGVVPPQ"
     gene            1326..3101
                     /gene="aspS"
                     /locus_tag="VFX94_10250"
     CDS             1326..3101
                     /gene="aspS"
                     /locus_tag="VFX94_10250"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX5768604.1"
                     /translation="MRSHYCGELRATLVDRTVTLCGWAHRRRDHGGVIFIDLRDREGL
                     AQVVCDPDAQEAFRTAERVRSEYVLKVTGKVRRRPAGTINSELPTGEVEVLARNVEIL
                     NASAPPPFQLDEDKLAEETRLQHRVLDLRREPMQRNLRLRHRAAMAVRRFLDEHGFVD
                     IETPVLYKSTPEGAREFLVPSRLHEGHFYALPQSPQLFKQLLMVAGFDRYYQIVKCFR
                     DEDLRADRQPEFTQVDIETSFLSEDDIRGLMESLIRFVFRETIGVELPAPFPVLTYED
                     AMRDYGSDKPDLRIPLRLAEITEAVKDVEFKVFSAPANAPGGRVSALRVPGGGAFTRS
                     ELDAYGEFAKSLGAKGLAWIKVNERAKGAEGLQSPIVKNLHTRAISAILDKTLSENGD
                     VILFGAGEADTVAGYMGALRDKVGHDRGLAVPGWRTLWVVDFPMFELDPETRQWKARH
                     HPFTAPKDDHEQVLEKDPGNALAKAYDVVLNGWEIGGGSVRIHRQEVQQTVFRALRMS
                     PEEQRAKFGFLLEALQYGAPPHGGIAFGFDRLVTLMAGAPAIRDVIAFPKTQRGQDLL
                     TGAPTPVTEKQLRELHLRLRKPGQP"
     gene            3098..3412
                     /locus_tag="VFX94_10255"
     CDS             3098..3412
                     /locus_tag="VFX94_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HEX5768605.1"
                     /translation="MSWLAVAVLAVALSSEIPVGKLPPEARQTLVLVKAGGPFPYQRD
                     GAVFYNREGRLPKRSRDYYREYTVKTPGANDRGARRIVAGQGGEFYYTDDHYRSFRRI
                     IE"
     gene            3409..3825
                     /locus_tag="VFX94_10260"
     CDS             3409..3825
                     /locus_tag="VFX94_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEX5768606.1"
                     /translation="MMSELLRFFQDASRSGVYRTARADDIRQAASGSGNRVATIAYGA
                     KPVLLKNIATALEFPDWFGENWDALEDCLSDLSWRKATGHSLLFEGAEVDDDFGILVE
                     VLRSVAGSWAARGTPFFAVFIDPAGALPLPEPGGPK"
     gene            3822..>3934
                     /locus_tag="VFX94_10265"
     CDS             3822..>3934
                     /locus_tag="VFX94_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEX5768607.1"
                     /translation="MKLPYSVLVVVHTEMEVLLLERAARPGFWQSVTGSLDR"
BASE COUNT          695 a         1268 c         1326 g          645 t
ORIGIN      
        1 gaaggacaag caaagcggcg agttgaagga agcgaccgag tggcatcgaa ttgcgttttt
       61 cggccggctt gccaccatcg caggcgaata cttgcgcaag ggctcgcagg tctacgtcga
      121 aggaagcctg cgcacgcgca agtggcagga caaggacggt caggaccgct actcgaccga
      181 gattcgcggc gatgtgatgc agatgctcgg ccggcgcgaa ggcggcggcg agccgcgcgt
      241 cgaagcggcg gcggagtcca agtccggcgc cgcgcgcagc ccggcaccgg ctgcggcgaa
      301 gaagccggca gggaagttcg acgacctgga agacgatatt cctttctgaa atcagcgata
      361 taatcgcttc catgccgatt tatccgtatc gctgtagcgc ctgcgggcag gagcacgatg
      421 tgctccagaa ggtgagcgag ccgccgctct ccgagtgccc ttcgtgcggc aaacccggga
      481 tgcagaagct cctcaccgcg gcggggttcc agttgaaggg aagcggctgg tacgcaaccg
      541 acttcaaggg cggggcgaaa aagcccgagg acaagaagca gcccgctgag ccgaagccgg
      601 ccgacagcaa gagcgatagc aagaccgaca ccaagacgag ccccaagagc gaaaccaaga
      661 ccgaggccgc ggcctgagcc gcggagcgta acgacgccgg cccggcgacg cgccgggcct
      721 ttttgtttcc ggagccgtga agaaatatct catcaccggg ctgcttatct ggatcccgct
      781 ggtcatcacc ctctgggtgc tgaagctcgt cgtggacgtg ctcgaccagt cgctcctgct
      841 gctgcccgag gcgtggcaga ccaagcactg gctcggcgtg cacgtcccgg gactgggcgc
      901 catcctcacc gtgttcatag tgttcctgac cggccttttc gccaccaact tcttcggcgc
      961 gcagctggtc gagctgtggc acgagatcct gcatcggatc ccggtggtga actccatcta
     1021 ctccagcgtg aagcagatct ccgacaccct gttctcctcg agcgggcagg cgttccgcaa
     1081 ggcgctgctg gtgcagtggc cgcaggaagg aatgtggacc atcgcttttc tcaccggtgc
     1141 gcccggcggc ggcgtggcgg agcacctgcc gcccgattgc gtcgcggtgt acgtgcctac
     1201 cactccgaac ccgacgggcg gctatttcgt catcgtggcg cgcaagaacg tcgtcgagct
     1261 cgacatgagc gtcgaccagg ccctgaaata catcatctcg atgggcgtcg tgccgccgca
     1321 atagcatgcg cagccactac tgcggcgagc tccgcgcgac cctcgttgac cgcaccgtta
     1381 cgctctgcgg ctgggcgcac cggcggcgcg accacggcgg ggtgatcttc atcgacctgc
     1441 gcgaccgcga gggtctcgcc caggtggtgt gcgatccgga cgcgcaagag gccttccgta
     1501 ccgcggagcg ggtacgctcc gagtatgtcc tgaaagtcac ggggaaggtg cggcgcaggc
     1561 ccgccggcac gatcaattcc gaactgccga ccggcgaggt agaagtgctc gcccgcaacg
     1621 tggagatcct caatgcctcg gcgccgcccc cgttccagct cgacgaggac aagctggcgg
     1681 aggaaacccg gctgcagcac cgcgtgctcg acctgcggcg cgagccgatg cagcgcaacc
     1741 tgcgcctgcg ccaccgcgcg gcgatggcgg tgcgccgctt cctcgacgag catggcttcg
     1801 tcgacatcga gacgccggtg ctttacaagt ccaccccgga aggcgcgcgc gaatttctgg
     1861 tcccttcgcg cctgcacgaa gggcatttct acgccttgcc ccaatcgccg cagcttttca
     1921 agcaactcct gatggtggcg ggcttcgacc ggtactacca gatcgtgaaa tgcttccgcg
     1981 acgaggacct gcgcgcagac cggcagccgg agttcacgca agtcgacatc gagacttcgt
     2041 ttctgtccga ggacgacatc cgcgggctca tggagtcgct gatccgtttc gtgttccggg
     2101 agacgatcgg cgtggagctg ccggcgccgt ttccggtgct gacgtacgag gatgcgatgc
     2161 gcgactacgg cagcgataag cccgacctgc gcatcccgct caggcttgcc gagatcaccg
     2221 aggcggtgaa ggacgtcgag ttcaaggtgt tctcggcgcc tgcgaacgcg cccgggggcc
     2281 gcgtcagcgc gctgcgcgtg ccgggcggcg gcgccttcac gcgcagcgag ctcgacgcct
     2341 atggcgaatt cgccaagagc ctgggcgcga aggggctggc gtggatcaag gtcaacgagc
     2401 gcgcgaaggg agcggagggg ctgcaatctc ccatcgtgaa gaaccttcac acgcgcgcga
     2461 tttcggcgat cctcgacaag acgctttccg agaatggcga cgtgatcctc ttcggcgcgg
     2521 gtgaagcgga caccgtcgcc ggatacatgg gagcgctgcg cgacaaggtc ggccacgacc
     2581 gcggccttgc cgtgcccggc tggcgaacgc tctgggtggt cgacttcccg atgttcgagc
     2641 tggaccccga gacccggcag tggaaggcga ggcatcatcc gttcaccgcg ccgaaggacg
     2701 accacgagca ggtgctcgag aaagaccccg gcaatgcgtt agccaaggct tatgacgtgg
     2761 tgctgaacgg ctgggagatc ggcggcggct cggtgcggat tcaccgccag gaggtgcagc
     2821 agacggtatt ccgcgcgctg cgcatgtcgc ccgaggagca gcgcgcgaag ttcggcttcc
     2881 tgctcgaagc gctgcagtac ggcgcgccgc cccacggcgg cattgccttc ggcttcgatc
     2941 gcctggtgac gctgatggcc ggcgcgccgg cgatccggga cgtcatcgcg tttcccaaga
     3001 cgcagcgcgg ccaggacctc ctcaccggcg cgcccacccc ggtgaccgag aagcagctgc
     3061 gcgagctgca tcttcgcctg aggaagcccg ggcagccgtg agctggcttg cggttgcggt
     3121 actcgctgtc gcgctctcca gtgagatccc cgtcggcaag ctgcctcccg aagcgcgcca
     3181 gaccctcgtg ctcgtcaagg cgggcggtcc gtttccttac cagagggacg gagccgtgtt
     3241 ctacaaccgc gaaggccggc tgcccaagcg cagtcgggac tactaccgcg aatacacggt
     3301 gaagacgccc ggggcgaacg atcgcggtgc gcgccgcatc gtcgccgggc agggcggcga
     3361 gttctattac accgacgacc attatcgaag cttcaggcgc atcatcgaat gatgagcgag
     3421 ctgctccgct ttttccagga tgcgtcgcgc tcgggcgttt accggaccgc gcgggccgac
     3481 gatatcaggc aggcggcaag cggcagcggc aaccgggtcg caacaattgc ctatggggca
     3541 aaacctgttc ttttgaaaaa cattgcaacg gcgctcgagt tcccggactg gttcggcgag
     3601 aactgggatg cgctcgagga ttgcctgtcc gacctctcct ggcgcaaggc aacgggccac
     3661 tccctgctct tcgagggggc agaagtggac gacgacttcg gcatcctggt cgaggtgctg
     3721 cgctcggtag ccggatcttg ggcggcgcgc ggcacgccgt tcttcgcggt gttcatcgat
     3781 ccggccggcg cgctgccgct gccggagccc ggcggcccga aatgaagctt ccgtattcgg
     3841 tgctggtggt ggtgcacacc gaaatggagg tactgcttct cgagcgcgcc gcgcggcccg
     3901 ggttctggca atcggtcacc ggctcactcg accg
//
LOCUS       DASUNT010000511         9502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_945255, whole genome shotgun sequence.
ACCESSION   DASUNT010000511 DASUNT010000000
VERSION     DASUNT010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9502
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945255"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VFX94_10270"
     CDS             <1..525
                     /locus_tag="VFX94_10270"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5768608.1"
                     /translation="APELSAVACAPAQAEPLPEAQREPLDGTQQALVGHLSKRFYIAP
                     AATERMVLAAYRAAAEVGLDPLVVLAVISIESRFNPIAESVMGAKGLMQIIPRYHLDK
                     LREAGGEEAVFDPESNIHIGARILQEYVYRTGTLEAGLQFYNGAFRDGSAQYAYKVLA
                     ERTRLEQVLRATSG"
     gene            522..947
                     /locus_tag="VFX94_10275"
     CDS             522..947
                     /locus_tag="VFX94_10275"
                     /inference="COORDINATES: protein motif:HMM:NF027482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
                     /protein_id="HEX5768609.1"
                     /translation="MKALLLALAFAVPAGTFAVETRLSGADVDEIRSVIHRQIDAFGR
                     DDAQGAFALVSPRVQQSFGSPERFLETMRTAYRPVYRPKGVSFLDLVVLSGEVVQRLQ
                     VTDRSGAVWVAYYVMQRQKDGSWRTHGCRLVPPTRTISA"
     gene            958..3015
                     /gene="glgX"
                     /locus_tag="VFX94_10280"
     CDS             958..3015
                     /gene="glgX"
                     /locus_tag="VFX94_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX5768610.1"
                     /translation="MEAPARAAVAPSAVWRGRPYPLGATWDGAGVNFALFSKHAEAVE
                     LCLLDPKGRREIERIAMRERTDFVWHCYLPEARPGQLYGYRVHGPHDPHRGHRFDHSK
                     LLLDPYARLIRGKVAAGLGKCEVVDTAFTWGDDRPPRTPWQDTVLYEMHVKGFTQLHP
                     QLPPQLRGTYAGLCSSTVLEYLKNLGVTAVELLPIHTIADERRLLHHGLRNYWGYNSI
                     GYFAPEMRYMSRGASRDPLGEFKSMVKTLHAAGIEVILDVVYNHTGEGDEHGPTLSFR
                     GIDNAIYYRLDPAHPRRYLNYTGTGNSFNLAHRVVLALVMDSLRYWVEEMHVDGFRFD
                     LAATLARNAHGAFDRNAAFVAAMRQDPVLSKVKLIAEPWDLAEGGYQLGNFPPGWAEW
                     NDKYRDAVRSYWKGDEGMLGKLASRLSGSQDIFQPSGRAPTASINFITTHDGFTLQDL
                     VSYERKRNDANLEGSRDGSDNNLSWNCGVEGPTDQVSIRNLRDQQKRNFLATLCFSQG
                     VPLLLAGDEMGRTQLGNNNAYCHDGALSWLDWELDADARSLADFTRKVLSLRKRHPLL
                     RRRTYPKPENTAWLSPEGREMTEEDWKLPFARCLGCLMVGERLAELDERGAPIVDDDL
                     LLLLNAHHDAVRFALPAGPWQIMLDTGRAQPAVEENSYPLDARSLALLARPRRTSPSR
                     TDG"
     gene            complement(2975..5512)
                     /gene="glgP"
                     /locus_tag="VFX94_10285"
     CDS             complement(2975..5512)
                     /gene="glgP"
                     /locus_tag="VFX94_10285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX5768611.1"
                     /translation="MQPPTSYALEVNPRIPKRLERLEELANNLWYSWNRPTRSLFARL
                     NPALWDAVGHTPKAMLKRIDEQRLIDAAADPAFLDSLSRVLAAFDAYHAEPPFKQNAG
                     GFRPEDLVAYFCAEFGMHESLPMYSGGLGILAGDHCKAASDFQLPFIAVGLLYRQGYF
                     TQTVDADGRQRAEYHDNDFDDMPIAPVRRADGAEITVELQFPGRKLMVKVWQARVGHV
                     TLYLLDTDLADNSERDRDIAHRLYGGDRVTRLEQELVLGVGGVRALAAIGHKPTVWHI
                     NEGHAAFLVLERVRVLTQEGLGVDAAVEAVASNTVFTTHTPVPAGHDHFSDAAVLPYL
                     KACLGEEKLTALGRPPAGGEFNMTALAIRGSRYHNGVSRMHGGVSARLLKEYWPDIAP
                     EENPVGYVTNGVHVPTFLAPEWSEVLDRFLGVGWMHRLGHPGLWEKIRGIPDHIFWSV
                     RQDIKARLFHMIRHRMSRQHARNHGSESHLERLLKHAHPANPNVLTVGFGRRFATYKR
                     ATLLFNDLDNLRRLFADRSRQVLFLFAGKAHPADEPGQEVIRTLVHLSGQEEFSGRVL
                     FIEGYDLHIARRMVSGVDVWLNNPVHPLEASGTSGMKAGMNGVINLSILDGWWDEGYD
                     GQNGWAIKPASPELDQHRRDREEARTLYEILQDRVIPLYYERTEAGFSPGWVRMAKRS
                     MASLLPRYDASRMLGEYVSKYYIPASRQGYRYTQNGNEAANSVAAWKAKVRAAWNGVA
                     ARRLDTPKPRIRFGESLPVEVAVKLNGLAPDDVCVELLLARGARQGAPVRHSHELKES
                     GPLGEGEHSFKSDLKPGLAGRLDYRIRVYPRHELLTHPFEMGLCAWV"
     gene            complement(5564..7399)
                     /locus_tag="VFX94_10290"
     CDS             complement(5564..7399)
                     /locus_tag="VFX94_10290"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX5768612.1"
                     /translation="MPAWASGVLVVSALLFALGYWTYSQVERSLREIRANTLQSLLDS
                     GVNALRVWINEEIADAQRLAREARVRAGIAALARSPECSGAARAGLEEVMRPLLREAG
                     DATFNVVARGGRLLATRFPGYCGREVSAAFLAELEPVFEGKPRFIRPFGDAERIANPP
                     ERLRSGAPFAWVYAPVRDNGRVIAALGIAQPVDAVFGSIIASARPGPRGEAFAFDRDA
                     KMLTAERFGESAPLARHPEGVALDPYESHSGVESIGAWRWLPEHAMGVAIEIGATEAY
                     APLRYLNIAFGVVFGALVLAVLAAAASALSVVRLRSELGGARKLGAYVLKKVIGEGGM
                     ATVYLARHDLLRRPAAVKLLKPARATDEMIARFEREVQMASSLAHPNTVEIFDFGRTR
                     DGLFYYAMEYLDGLTVGDIITRHGHVTVARTIHVLRQVCAALAEAHAKGVVHRDIKPE
                     NIMVCRYGGAYDHVKILDFGLVKHIAEKHSRDLTRTLRILGTPLYMAPERLRNPADVD
                     AHVDIYALGAVAFLMLTGRKLFESADDLQLTSKILNEEPPRASSVAPQPIPVELDLLV
                     LSCVEKRREDRPQRIADLVEAFDALALEHRWTQREAESWWKEKSP"
     gene            complement(7453..8400)
                     /locus_tag="VFX94_10295"
     CDS             complement(7453..8400)
                     /locus_tag="VFX94_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5768613.1"
                     /translation="MLVKADFQHPAVAASPIDRVVGAASGIILGKETQVRLALACLLA
                     RGHLLIEDLPGVGKTTLAHVLARALGLHFQRIQFTSDMLPADILGVSIYERDSGGFKF
                     HPGPIFSQVILADEVNRATPKTQSALLEAMEEHQVTAEGETRKLSEPFFVIATQNPSE
                     QVGTFPLPESQLDRFMMRIELGYPDRDAERALLCGADRRDLLATLQPCMTPAELVELQ
                     ENVQQIHVSPALLDYVQAIVEHTRRSPEYLTGLSPRAALALVHSAKAWALLEGRDKVI
                     PEDVQAIVPGVAAHRLRPAHDAGRRVDVAAQLLSAVPIP"
     gene            8439..9365
                     /locus_tag="VFX94_10300"
     CDS             8439..9365
                     /locus_tag="VFX94_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX5768614.1"
                     /translation="MPAAFVTHPDCLRHEMGVHHPERPERLSAIEDQLIASGLGQYLA
                     RYDAPLASDEQLGRVHPLEYVKAIRESAPSAGTVHLDPDTAMNPFSLQAALRAAGAAV
                     LATDLVLTRKHNSAFCAVRPPGHHACRARPMGFCIFNNVAVAARHALHSHGLERVAII
                     DFDVHHGNGTEDIFEGDEQVLMASIFQHPFYPYSGTDSPARNMHNVPLAAGSGSKELR
                     KAVIEEWLPALEEFRPELIAFSAGFDAHIEDDMAMLRFTDADYAWVTEQVKGVADRHA
                     KGRMVSMLEGGYALSALGRSAVQHIKVLAGLN"
BASE COUNT         1544 a         3214 c         3229 g         1515 t
ORIGIN      
        1 gcgcccgagc tctccgcagt cgcatgcgcg ccggcgcagg ccgagccgct gccggaagcg
       61 cagcgcgagc cgctcgacgg cacgcagcag gcgctcgtcg gacatctttc gaagcgcttt
      121 tacatcgctc cggcggccac cgagcgcatg gtgctcgccg cgtaccgggc ggctgcggag
      181 gttggcctcg atccgctggt ggttctcgcc gtgatctcga tcgagtcgcg cttcaacccg
      241 attgcagaga gcgtcatggg cgccaagggc ctgatgcaga tcattccccg ctaccacctc
      301 gacaagctgc gcgaggcggg tggcgaggag gccgttttcg acccggagag caacatccac
      361 atcggcgccc gcatcctgca ggaatacgtc taccgcaccg gcacgctgga ggcggggctg
      421 cagttctaca acggcgcctt ccgcgacggc tcggcgcagt acgcgtacaa ggtgctcgcg
      481 gagcgcaccc ggctcgagca ggtgctgcgc gcgacaagcg gatgaaggcc ttgctgcttg
      541 cgctggcgtt cgccgttccg gcggggacct tcgcggtcga gacccgcctg agcggcgccg
      601 acgtggacga gatcaggtct gtcatccacc ggcagatcga cgcttttggg cgcgacgacg
      661 cccagggcgc cttcgcgctc gtgtcgccgc gtgtgcagca gagcttcggc tcgcccgagc
      721 gcttcctcga aacgatgcgc acggcgtacc ggccggtgta ccgcccgaaa ggcgtgtcgt
      781 tcctcgacct tgtggtgctg agcggcgaag tggtccagcg gctgcaggtc accgaccgct
      841 ccggcgcggt ctgggtggcg tattacgtga tgcaacggca aaaggatggc agctggcgca
      901 cccacggctg ccggctcgtt ccgcccaccc gcacgatttc tgcataatcg ggcgcgagtg
      961 gaagcgcccg cacgagccgc ggtcgcgccg agcgcggtct ggcgcggccg cccctatccc
     1021 ctgggagcga cctgggacgg ggccggcgtg aacttcgcgc tgttctccaa gcacgccgag
     1081 gcggtggagc tgtgcctgct cgacccgaag ggccggcgcg agatcgaacg catcgcgatg
     1141 cgtgagcgca ccgatttcgt ctggcactgc tacctccccg aggcgcggcc ggggcagctc
     1201 tatggctacc gcgtgcacgg gccgcacgac ccccaccgcg gccatcgctt cgatcacagc
     1261 aagctcctcc tcgacccgta tgcccgcctg atccggggaa aggttgccgc cgggctcggc
     1321 aaatgcgagg tcgtcgacac ggcgttcacc tggggcgacg accgcccgcc gcgcacgccg
     1381 tggcaggaca cggtgctcta cgagatgcac gtcaaggggt tcacccagct ccatccccag
     1441 ctgccgccgc agctgcgcgg tacctatgca ggactttgca gttcgacggt cctcgagtac
     1501 ttgaagaacc tcggggtcac ggcggtggag ctccttccga tccatacgat cgcggacgag
     1561 cgccggctgc tgcaccacgg cctgcgcaac tactggggct ataactccat cggctacttc
     1621 gccccggaga tgcgatacat gtcgagaggc gcttcgcgcg acccgctcgg cgaattcaag
     1681 agcatggtga agacgctgca cgccgccggg atcgaggtga tcctcgacgt cgtctacaac
     1741 cacaccggcg agggtgacga acacgggccg acactcagct ttcgcggcat cgacaacgcc
     1801 atctactacc gcctcgaccc ggcgcacccg cgccgctatc tcaactacac gggcaccggc
     1861 aacagcttca acctggcgca ccgggtggtg ctcgcgctcg tcatggacag cctgcgctac
     1921 tgggtcgagg agatgcacgt cgacggcttc cgctttgacc tggccgccac gctcgcgcgc
     1981 aacgcgcacg gcgcgttcga ccgcaacgcc gccttcgtcg ccgcgatgcg ccaggatccg
     2041 gtgctgtcca aggtcaagct gatcgccgag ccgtgggacc tcgccgaagg cggctaccag
     2101 ctcggcaact tccccccggg ctgggcggag tggaacgaca agtaccgcga cgccgtgcgt
     2161 tcctactgga aaggcgacga aggcatgctc ggcaagctcg cctcgcggct ctccggctcg
     2221 caggacatct tccagccgag cgggcgcgcg cccacggcga gcatcaactt catcacaacg
     2281 cacgacggct tcacgctgca ggacctggtg agctacgagc gcaagcgcaa cgacgccaac
     2341 ctcgagggca gccgcgacgg ctcggacaac aacctcagct ggaactgcgg cgtcgagggg
     2401 cccacggacc aggtttcgat aagaaacctg agagatcaac aaaagcgtaa tttcctcgcg
     2461 acgttgtgct tttcgcaggg cgtgccgctg ctcctcgcgg gcgacgaaat ggggcgcacg
     2521 cagctcggca acaacaacgc ctactgccac gacggcgcgc tctcctggct cgactgggag
     2581 ctcgacgccg acgcgcgctc gctcgccgac tttacgagga aggttctttc actgcgaaag
     2641 cgccatccgc tccttcgccg ccgcacctac ccgaagcccg agaacaccgc ctggctctcg
     2701 cccgagggac gcgagatgac cgaggaggac tggaagcttc ccttcgcgcg ctgcctcggc
     2761 tgcctcatgg tgggcgagcg cctcgcggag ctcgacgagc ggggcgcacc gatcgtcgat
     2821 gacgatctgc tgctcctcct gaacgcacat cacgacgcag tgcgcttcgc ccttcccgca
     2881 gggccgtggc agatcatgct cgacaccggg cgagcgcagc ccgcggtcga ggagaacagc
     2941 tatcctcttg atgcgcgctc gcttgcgctg ctcgctagac ccaggcgcac aagcccatct
     3001 cgaacggatg ggtgaggagc tcgtggcgcg ggtagacgcg gatgcggtag tcgagccggc
     3061 cggcgaggcc gggcttcagg tcgcttttga acgaatgctc cccctcgccg agcgggcctg
     3121 attctttcaa ttcatgcgaa tgccgtaccg gcgcgccctg gcgcgcgccg cgggcgagga
     3181 ggagctcgac gcagacatcg tcgggtgcca ggccgttcag cttgacggcg acttcgacgg
     3241 gaagcgattc gccgaaccgg atgcggggct tcggcgtatc gagccgccgg gcggccacgc
     3301 cgttccaggc cgcgcgcacc ttcgctttcc acgccgcgac cgagttcgcg gcctcgttgc
     3361 cgttctgcgt gtagcggtag ccctggcgcg aggccgggat gtagtacttc gagacgtact
     3421 cgcccagcat gcgagaggcg tcatagcgcg gtagcagcga tgccatcgag cgcttggcca
     3481 tccttaccca gcccggcgag aagccggcct cggtacgctc gtagtagagc gggatgacgc
     3541 gatcctggag gatctcgtag agagtgcgtg cctcctcgcg gtcgcgtcgg tgctggtcga
     3601 gctcaggcga ggcgggcttg atcgcccagc cgttctggcc gtcgtagccc tcgtcccacc
     3661 agccgtcgag tatcgagagg ttgataacgc cgttcatgcc cgccttcatt cccgaagtgc
     3721 cagaggcttc gagcgggtgc accgggttgt tgagccacac gtctacgccg gacaccatgc
     3781 gccgcgcgat gtgcaggtca tagccttcga tgaagagcac gcggccggag aactcctcct
     3841 ggcctgagag gtgcaccagc gtgcggatca cctcctgccc gggctcgtcg gccgggtgtg
     3901 ccttgccggc gaagaggaag agtacctggc gcgagcgatc ggcgaagagc cggcgcaggt
     3961 tgtcgaggtc gttgaagagc agcgtcgcgc gcttgtaggt cgcgaagcgc cggccgaagc
     4021 ccacggtgag cacgttcggg tttgccggat gggcgtgctt cagcaggcgc tcgaggtgcg
     4081 attcgctgcc gtggttgcgc gcgtgctgcc gcgacatgcg gtgccggatc atgtgaaaca
     4141 ggcgcgcctt gatgtcctgg cgcacgctcc agaagatgtg gtcgggtatg ccgcggattt
     4201 tttcccagag tcccgggtgc ccgaggcggt gcatccagcc cacgccgagg aagcggtcga
     4261 gcacctcgct ccattcgggc gcgaggaagg tcggtacatg cacgccgttc gtcacgtagc
     4321 cgaccgggtt ctcctccggg gcaatgtccg gccagtactc cttgagcagc cgcgccgaca
     4381 cgccaccgtg catgcgcgac acgccgttgt ggtaacgcga gccgcggatc gcgagcgcgg
     4441 tcatgttgaa ttcgccgccc gccggcggtc ggccgagtgc cgtgagcttc tcctcgccca
     4501 ggcacgcctt gaggtacggc agcacggcgg cgtcggaaaa atggtcgtgc ccggcaggga
     4561 ccggggtgtg agtcgtgaac acggtgttcg aagccaccgc ctcgaccgcg gcgtccacgc
     4621 cgagtccttc ctgcgtcagc acacgcacgc gctccagcac gaggaacgcg gcgtgccctt
     4681 cgttgatgtg ccatacggtc ggcttgtggc cgatcgccgc gagggcgcgc acgccgccca
     4741 cccccagcac gagctcctgc tcgaggcgcg tgacgcggtc tccgccgtag agacgatgag
     4801 cgatatcgcg atcgcgctcg ctgttgtccg cgaggtcggt gtcgagaagg tagagcgtga
     4861 cgtggccgac gcgcgcctgc caaaccttca ccatcagctt cctgccggga aactgcagct
     4921 cgaccgtgat ttccgcgccg tcggcgcggc gcaccggcgc gatgggcatg tcatcaaagt
     4981 cgttgtcgtg gtattccgcg cgctggcgcc cgtcggcgtc aacggtctgc gtgaagtagc
     5041 cctggcggta cagcagcccg accgcgatga acgggagctg aaagtcgctc gccgccttgc
     5101 aatggtcgcc ggcgaggatg ccgagcccgc ccgagtacat cggcaggctc tcgtgcatgc
     5161 cgaattcggc gcagaagtac gccacgaggt cctccggccg gaacccgccc gcgttctgct
     5221 tgaacggcgg ctcggcgtgg taggcgtcga acgcggcgag cacgcgagaa aggctgtcca
     5281 gaaatgcggg gtcggcggcc gcgtcgatca ggcgctgctc gtcgatgcgc ttgagcatcg
     5341 ccttgggcgt atgcccgacg gcgtcccaca gcgcggggtt cagccgagcg aaaagcgagc
     5401 gcgtcgggcg gttccagctg taccagaggt tgttcgcgag ctcctccagc cgctcgaggc
     5461 gtttcgggat gcgcgggttg acttcgagcg catagctggt cggcggctgc acggagccgg
     5521 gtagtctacc gaatggcgac agaaaaaggg gtcaggactt ttttcacgga gatttttcct
     5581 tccaccacga ttccgcctcg cgctgcgtcc agcgatgctc gagcgcgagc gcgtcgaatg
     5641 cctcgaccag atccgctatg cgctgcggcc ggtcttcgcg ccgcttctcg acgcacgaaa
     5701 gcacgagcag gtcgagctcc acggggatcg gctgcggcgc cacgctggaa gcgcgcggcg
     5761 gctcctcgtt caggatcttg ctcgtgagct ggaggtcgtc cgcgctttcg aaaagctttc
     5821 ggccggtcag catgagaaag gcgaccgcgc ccagtgcgta gatgtcgacg tgcgcgtcga
     5881 cgtcggccgg attgcgcagc cgctccggcg ccatgtagag cggggtgccc aggatgcgca
     5941 gggtgcgggt gaggtcgcgg ctgtgcttct cggcgatgtg cttcaccagc ccgaaatcga
     6001 ggatcttcac gtggtcgtac gccccgccgt agcggcacac catgatgttt tcgggcttga
     6061 tgtcccggtg caccacgcct ttggcgtggg cttcggcgag cgccgcgcac acctggcgca
     6121 ggacatgaat ggttctggcc acggtcacat gaccgtggcg ggtgatgata tcccccacgg
     6181 tcagaccatc gaggtactcc atcgcgtagt agaacagtcc gtcgcgcgtg cggccgaaat
     6241 cgaagatctc caccgtattg ggatgcgcca ggctggaggc catctgcacc tcacgctcga
     6301 agcgcgcgat catctcgtcg gtggcgcgcg cgggcttgag cagcttcacc gcggccggcc
     6361 gcctcagcag atcgtggcgc gcgaggtaga cggtggccat cccgccctcg ccgatgactt
     6421 tcttcagcac gtaggcgccg agcttgcgcg ccccgccgag ctcgctgcgc aggcgcacca
     6481 ccgagagcgc gctcgccgcg gcggcgagca ccgcgagcac gagcgcgccg aataccacgc
     6541 cgaacgcgat attgaggtag cgaagcggcg cataagcctc ggtggcgccg atctcgatcg
     6601 cgacgcccat tgcgtgctcg ggcagccagc gccaggcgcc gatcgactcg acgccgctgt
     6661 ggctctcata agggtcgagc gccacgcctt cgggatgccg ggcgagcggc gcgctctcgc
     6721 cgaagcgctc ggcggtcagc atctttgcgt cgcggtcgaa ggcgaatgcc tcgccgcgcg
     6781 gaccgggccg cgcggaagcg ataatcgagc cgaacaccgc gtccaccggc tgcgcgatgc
     6841 cgagggccgc aatcactctg ccgttgtcgc gcaccggcgc gtacacccag gcgaacgggg
     6901 cgcccgagcg aaggcgctcc ggcggattgg cgatgcgctc ggcgtccccg aacggccgga
     6961 tgaagcgcgg cttgccttcg aacaccggct cgagctcggc gagaaacgcc gcgctcacct
     7021 cccggccgca gtagcccggg aagcgcgtgg cgaggagccg cccgccgcgc gccaccacgt
     7081 tgaacgtcgc gtcgccggct tcgcgcagaa gcgggcgcat gacttcctcg aggcccgcgc
     7141 gcgccgcacc ggagcattcg ggactgcgcg caagcgcggc gatgccggcg cgcacgcgcg
     7201 cttcgcgtgc gaggcgctgc gcatcggcga tttcctcgtt gatccagaca cgcaatgcgt
     7261 tcacgccgga atcgagcagg ctctgcagcg tgttggcgcg gatctcgcgc agcgagcgct
     7321 cgacctggct gtaggtccag tagccgagcg cgaagagcag cgccgagacg acgagcacgc
     7381 cgctcgccca ggcgggcacg gaaatcagcc gcttttgcgg ctcttccggc gccgggtgaa
     7441 gggtatcttc ggctatggga tcggaaccgc cgacagcaac tgcgccgcca cgtcgacgcg
     7501 gcggccggcg tcgtgcgccg ggcgcaggcg gtgcgcggcg acgccgggca cgatcgcctg
     7561 cacgtcctcg gggatcacct tgtcgcgtcc ctcgagcagc gcccaggcct tcgccgagtg
     7621 aacgagcgcg agcgccgcgc gcggcgagag gccggtcagg tattccggcg agcgccgggt
     7681 atgctctacg attgcctgca cgtagtcgag cagcgccggc gacacgtgga tctgctgcac
     7741 gttctcctgc agctcgacga gctcggccgg cgtcatgcac ggctgcagcg tggcgagcag
     7801 atcacggcgg tcggcgccgc agaggagcgc gcgctcggcg tcgcggtccg ggtagccgag
     7861 ctcgatcctc atcatgaagc ggtcgagctg cgactcgggc agcgggaagg tgccgacctg
     7921 ctccgacggg ttctgggtgg cgatgacgaa gaagggctcg gagagcttgc gcgtctcgcc
     7981 ctcggccgtc acctggtgct cctccatcgc ctcgagcagc gcggactgcg tcttcggcgt
     8041 cgcgcggttc acctcgtcgg cgagaatgac ttgcgaaaaa atcggcccgg ggtggaactt
     8101 gaagccgccc gagtcgcgct cgtagatcga cacgcccagg atgtcggcgg gcagcatgtc
     8161 gctcgtgaac tggatgcgct ggaagtgcag cccgagcgcg cgggcgagca cgtgagcgag
     8221 ggtggtcttg ccgacgccgg gcagatcctc gatgagcagg tgcccgcgcg cgagcaggca
     8281 cgcgagcgcg aggcgcacct gcgtctcctt gccaaggatg atgccgctcg cagcgccgac
     8341 tactcggtcg atcggcgatg ccgcgacagc cggatgctgg aagtcggcct tgacaagcat
     8401 gcgacgattg tactcgatgc gttaagatgg aaattcgcat gcctgccgca tttgtcacac
     8461 atccggattg cctacgccac gaaatgggcg tgcatcatcc cgagcgtccc gagcgcctct
     8521 cggccatcga ggaccagctc atcgcttccg gcctcggcca gtacctcgcg cgctacgacg
     8581 cgccgctcgc gagcgacgag cagcttggcc gcgtgcatcc gctggagtac gtgaaagcga
     8641 tccgcgagtc cgcgccgagc gcggggacgg tgcatctcga tccggacacc gcgatgaacc
     8701 cgttcagcct gcaggccgcc ctgcgcgccg ccggcgccgc ggtcctcgct accgacctcg
     8761 tgctgacaag aaagcacaat tccgccttct gcgcggtgcg gccgccggga catcacgcct
     8821 gccgcgcgcg gccgatggga ttctgcatct tcaataacgt agcggttgcc gcgcgccacg
     8881 cgctccactc gcacggcctc gagcgcgtcg cgatcatcga cttcgacgtg caccacggca
     8941 acggcacgga ggacatcttc gagggcgatg agcaggtgct gatggcctcg atcttccagc
     9001 acccgttcta tccttacagc ggcaccgaca gcccggcgcg caacatgcac aacgtgccgc
     9061 tcgcggcagg atcgggctcc aaggagctca ggaaagccgt gatcgaggaa tggctgcctg
     9121 cgctggagga gttcaggccc gagctgattg ccttctccgc cgggttcgat gcgcacatcg
     9181 aagacgacat ggcgatgctg cgcttcaccg atgccgatta cgcctgggtc accgagcagg
     9241 tgaaaggcgt ggccgatcgc cacgccaagg gaagaatggt ctcgatgctc gagggcggct
     9301 acgccctctc ggcgctcggc cgcagcgccg tgcagcacat caaagtcctt gcgggactca
     9361 actaagtttc ccgcacgcgc aagacccgga acaccgccat ggggcgatgg aagcccttga
     9421 gagcgagctc gcccagcggc tgggtctccg ccagctcgga aaccgccgcc tggacgcgct
     9481 ggctgatcag gatttctccc cc
//
LOCUS       DASUNT010000512         3177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_858323, whole genome shotgun sequence.
ACCESSION   DASUNT010000512 DASUNT010000000
VERSION     DASUNT010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858323"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VFX94_10305"
     CDS             complement(<1..268)
                     /locus_tag="VFX94_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5768615.1"
                     /translation="MLLEVQKLRHVYGDHEVIAGLSFSLERGAIGCLLGPSGGGKTTV
                     LRCIAGFEAVQAGEIRLAGRVVSAPGKTVAPERRRIGMVFQDYAL"
     gene            315..1937
                     /locus_tag="VFX94_10310"
     CDS             315..1937
                     /locus_tag="VFX94_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5768616.1"
                     /translation="MNYKNLGLLGSIATLIAAIVALPVVLVVLSVFSGAGETWRHLAA
                     TALPRYVLNTGLLLALVSFGVVSIGVVSAWLVTAYRFPGRALLEWALVLPLAMPAYVM
                     AYAYTDWLQFTGEVQTALRELTGWRARDYWFPEVRSLPGAAVMLSFALYPYVYLVART
                     AFLDLSRSAIEAGRLAGYGPLGAFTRVAVPLARPAIVAGSALALMETLADFGTVSYFG
                     VEVFTTGIFKAWLSMGDSVAAAQLSACLLAFVVLLLALERVNRGRAAYHGPSPRRTRL
                     HQLRGVGAVAAFVACAAPVVFGFAFPALLLARLSWQETTSAARLAGLVGNSFSIAALT
                     AFAAVAAALIMAYAARLTRSPLVAFANRVAGLGYAVPGAVIAVGILIPLGRLDNALAG
                     WLESSFGIKAGLLLTGTIAALVYAYLVRLLAVSLQTTDAGLAKITPSMEDAARSLGAA
                     PAATLARVHAPLLAPSLATAALLVFVDVLKELPATFALRPFNFDTLAVEAYNLAKDER
                     LAEAAAPSLAIVLAGLLPLIIVSKRYFQATRS"
     gene            complement(1913..2944)
                     /locus_tag="VFX94_10315"
     CDS             complement(1913..2944)
                     /locus_tag="VFX94_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5768617.1"
                     /translation="MTVSLTKKIAAGLFSLAVAGAATSQQNVVNVYSGRHYQTDEALY
                     AGFIKATGIKVNRIEASEDAIIERIRNEGERSPADVMITVVAGRLWRADQLALFQPAK
                     SATLEKRIPESLRHPEGHWFGFSTRARVIAYNKERVKPGEISTYEELADPKWKGRVCM
                     RSSTSIYNLSLMGALIDHLGESRAEAWAKGVRENLAQDPKGGDTDQLKAVAAGLCDVT
                     VSNQYYYARLARSAKADEKQVADKLGIVFPNQASWGTHVNISGAGVLKHAPHRAAAVR
                     FLEYLASDEAQRYFADGNNEWPAVRGVKVDNPVLNALGDFKADRINVAVLGRNQASSQ
                     KIYDRVAWK"
BASE COUNT          468 a         1064 c         1019 g          626 t
ORIGIN      
        1 agagcgcata gtcctggaac accatgccga tgcgccgccg ctcgggcgca accgttttgc
       61 cgggcgccga caccacgcgt cccgcaaggc ggatttcgcc cgcctgaacc gcttcgaacc
      121 cggctatgca gcgaaggact gtagtcttcc cgccgccgct cggtccgagg aggcagccga
      181 tcgcgccgcg ctctagagaa aagctgagtc ccgcgatcac ctcatgatcg ccgtagacgt
      241 ggcgcagctt ttgaacttcg aggagcacag ggcattatag taataattct cattaacgtt
      301 aaaatcgcag cgccttgaat tacaagaact tgggcctgct ggggagcatc gccactctga
      361 tcgccgcaat cgtggcgctc cccgtcgtcc ttgtggtcct cagcgtgttc agcggcgccg
      421 gcgagacgtg gcgacatctc gcggccaccg cgcttccgcg ttacgtgctc aacacggggc
      481 tacttctcgc gctggtgagc ttcggcgtgg tgtcgatcgg cgtggtgagc gcctggctcg
      541 tcaccgcgta ccgctttccg ggccgagcgc tgctcgaatg ggcgctcgtc ctgccgctcg
      601 ccatgcccgc ttacgtgatg gcttatgcct acaccgactg gcttcagttc accggagagg
      661 tgcagacggc gctgcgcgag ctcaccggct ggcgtgcgcg tgactactgg tttcccgaag
      721 tgcgctcact gccgggcgcc gccgtcatgc tgtccttcgc gctttacccg tacgtatatc
      781 tcgtggcgcg caccgcgttt ctcgacctct cgcgcagcgc catcgaggcg gggcggctcg
      841 cgggctacgg cccgctcgga gctttcaccc gcgtcgcggt gccgctcgcg cggccggcca
      901 tcgtcgcggg cagcgcactc gcgctcatgg aaacgctcgc cgacttcggc accgtgtcgt
      961 atttcggcgt ggaggtcttc accaccggca tcttcaaggc atggctctcg atgggcgaca
     1021 gcgtagcggc cgcgcagctc tcggcatgcc ttctcgcctt cgtcgtgctg ctcctcgcgc
     1081 tcgagcgcgt gaatcggggg cgcgccgcgt accacggccc ctcgccgcgc cgaacacggc
     1141 ttcatcagtt gcgcggagtg ggggctgtcg ctgcattcgt cgcctgcgca gcgccggtgg
     1201 tgttcggctt tgcatttccc gcgctgctgc tcgcacggct gagctggcag gagacgactt
     1261 cggcagcccg gctcgccggg ctggtcggga acagcttcag catcgccgcg ctcaccgcgt
     1321 tcgccgctgt ggcggcggcg ctcatcatgg cctacgccgc gcgtcttacg cgaagccctc
     1381 tcgtcgcatt cgcgaaccgg gtggcaggac ttggctatgc ggtgcccgga gcggtgatcg
     1441 cggtcggcat cctcattccg ctcgggcggc tcgacaacgc gctcgccggc tggctcgaga
     1501 gcagcttcgg aatcaaggcg gggcttcttc tcaccggaac gatcgccgcg ctcgtctacg
     1561 cgtacctggt gcggttgctt gcggtgtcgc tccagacgac cgacgccggg ctcgcgaaaa
     1621 tcacgccgag catggaggat gccgcgcgct cgctcggcgc ggcgcccgcc gcgacgctcg
     1681 cccgggtgca tgcgccgctt ctcgcgccca gcctcgcgac ggcggcgctc ctcgtattcg
     1741 tcgatgtgct taaggagctg ccggcgacct tcgcgctgcg gccgttcaac ttcgatacgc
     1801 tcgcggtgga ggcctacaac cttgcgaagg acgagcgcct ggcggaggcc gccgcgccgt
     1861 cgcttgcgat cgtactggcg ggattacttc cgctcatcat cgtgtcgaag cgctatttcc
     1921 aggcgacgcg atcgtagatt ttctgcgagc tggcctggtt cctgccgagc accgcgacgt
     1981 tgatgcggtc ggccttgaaa tcgccgagcg cattgagcac cgggttgtcg accttcacgc
     2041 cgcgcaccgc gggccactcg ttgttgccgt cggcgaagta gcgctgcgcc tcgtcgctcg
     2101 cgaggtactc caggaatctg accgcggccg cgcggtgcgg cgcgtgcttc agcaccccgg
     2161 cgcccgaaat gttgacgtgc gtgccccagc tcgcctggtt gggaaacacg atgccgagct
     2221 tgtcggcgac ctgcttctca tcggccttgg ccgagcgcgc aaggcgcgcg tagtaatact
     2281 ggttcgagac cgtgacgtcg cacagtcctg cggcaaccgc cttgagctga tcggtatcgc
     2341 cgcctttagg atcctgcgcg aggttctcgc gcacaccttt cgcccacgcc tcggcgcggg
     2401 attcgcccag gtgatcgatc agcgcgccca tgagcgaaag gttgtagatg ctggtcgagg
     2461 agcgcatgca aacgcggcct ttccatttcg ggtcggcgag ctcctcatag gtgctgatct
     2521 ctcccggctt cacgcgctcc ttgttgtagg cgatgacccg ggcacgcgtc gaaaagccga
     2581 accagtgccc ctcgggatgc cgaaggctct cgggaatgcg cttctcgagc gtcgccgatt
     2641 tggccggctg gaagagggcg agctgatcgg cacgccacag gcgccctgcg acgacggtaa
     2701 tcatgacgtc ggccggactg cgctcgcctt cgttccggat tcgctcgatg atcgcgtctt
     2761 cgctcgcctc gatccggttc accttgatcc cggtcgcctt gatgaacccg gcgtacagcg
     2821 cttcgtcggt ctggtagtgg cgcccggagt aaacgttaac tacgttttgc tggctggtgg
     2881 ccgctccagc aacggcgagg ctgaacagcc cggccgcaat ctttttagtc agcgaaacag
     2941 tcatgtggca cccgggtgag aatgcacaac aaatagtaat tactctcatt tacgccgtca
     3001 acgagaatta ctatcatttg ccatgaaggc cgtgggaatc tcgcgcctcg ttcgcagaga
     3061 cggcgcgccc gctgggccct gttgggagat gaagtgcgca gccgcgtgaa tccggctggc
     3121 acacgccatt gaacgccgtg ggttagccta gagcggctga ctcgtgcacc ctctggc
//
LOCUS       DASUNT010000513         5442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_683275, whole genome shotgun sequence.
ACCESSION   DASUNT010000513 DASUNT010000000
VERSION     DASUNT010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683275"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VFX94_10320"
     CDS             <1..419
                     /locus_tag="VFX94_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5768618.1"
                     /translation="ITFITFLGAFYTIVINVLGGARSIDVRYFQAASSMGSSRWDIFR
                     RIILPGTLPSIVVGSAVGMGITWEVVVAAEMISGGGSQQAGVGGGLGFFIWNSYVGGS
                     YEQIVVGMISIGIAGFISSELLRWGGRYATPWLKLR"
     gene            401..1270
                     /locus_tag="VFX94_10325"
     CDS             401..1270
                     /locus_tag="VFX94_10325"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5768619.1"
                     /translation="MAETAVRPKIVPGEITVDAVTKSYGALQFRKDVVQECSFTIERA
                     KLTVMVGPSGCGKSTLIRLLAGFEQPTAGRILLDGAPVAAPSRDRLVVFQETALFPWM
                     TTWNNILYGPRARGELTRETQQFGEFLLAKVGLQEFRDKYPGQLSGGMQRRAELARAM
                     INNPKVMILDEPFRGLDAMTKELMWEYYSRLYEENRRTNFFVTTDIDEAVFLADRILV
                     MSNIPTQVRATLEVDLPRPRKLLDIFESDRANDIKKQALSILHAEAMRSFSRGSKAAA
                     DFVEAYLKRASRA"
     gene            1267..1470
                     /locus_tag="VFX94_10330"
     CDS             1267..1470
                     /locus_tag="VFX94_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768620.1"
                     /translation="MMEIVLTKPAVVALAVLGALLCTGASFLQMRGYVSEKAGKALNW
                     AGYGVMGASILLFVLAGLRGVQP"
     gene            complement(1477..2355)
                     /locus_tag="VFX94_10335"
     CDS             complement(1477..2355)
                     /locus_tag="VFX94_10335"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEX5768621.1"
                     /translation="MSALEEHLATRRSAGLFDFSFMGLYEFDDKAELQKLQTRELDAL
                     VPGQIAYTLLLKQDGAVLIDATVWRLDERRYWLFSGKRARYGGRDRSGEYAILALQGP
                     ASGRILARLIGAQAVRALNYFRFIQTPLLVGRLGYSGELGYELLVPDADAAALGRRLL
                     DAGRSDDLRDCTWAAANSLRIEAGYVLFDREVDGRANPRELGLERLAPGQAFPMTRRF
                     VGLEIFDEAVDAEYPVAQLTSECYSPTLAKRIALGFVAPGIAEGAGVRAGARLARVAR
                     LPFYDAERRRPRAAPL"
     gene            complement(2352..3098)
                     /locus_tag="VFX94_10340"
     CDS             complement(2352..3098)
                     /locus_tag="VFX94_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5768622.1"
                     /translation="MTASLLLAAVAAFVGLLIGTVGVGGVLMVSYLALFAGLTIHQAA
                     ATSLFSFLFTGILGTWLYQRRGSIDWRIAAPVCGAALVCGFLGAALAARLDPRPLAIA
                     IALLIVLAGVYVLVPQRASAQPRDGRRRGEAWLLAGVGAASGFGSGFSGAGGPLFSVP
                     LMVILRYVPLTAVATSQALQIVAATSGSLENLRNGFIDWDTAVLVTVFELAGVLVGVR
                     LAHRASALALRRLAGALCVITGALLLARSL"
     gene            complement(3095..3547)
                     /locus_tag="VFX94_10345"
     CDS             complement(3095..3547)
                     /locus_tag="VFX94_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5768623.1"
                     /translation="MSGALSLEPARVLEQARFFSGLDRAQRERVAALGRARSFSQDTR
                     IYTVGDRVDDFYVLADGMVRFTLGLGKRDTAAGDIIRRGDVFGWAPLVEGHARRIATA
                     YCLTPCEVIAIDGGALTRLMAADTGVGYALMTKLAVLLTSELVAFAAG"
     gene            complement(3544..4437)
                     /gene="panB"
                     /locus_tag="VFX94_10350"
     CDS             complement(3544..4437)
                     /gene="panB"
                     /locus_tag="VFX94_10350"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX5768624.1"
                     /translation="MADAKPAQPARKKVTIPMLVEKMKKGEPIVQLAVYDYETAIIAD
                     RVGIDILCVSDTGGMVLFGHETTTSVSFEEVMFMAQAVKRGSRFGLRMVDMPYMSFHL
                     SAQQAVDNAAKYVSQGGAEVMKCEGNQHHAKYIEAIVKAGIPVQGHIGITPMRMPQLG
                     GFFAQGKTAERAKELIDDAWAMVDAGCFSIMCEVTTSEVCEYLAETLPVPVISLGAGN
                     RAHGVHIIGSDLFHLYEAHVPRHSKIYVDLVPILEKGLSDYRDDVRARRYPGPEHTVF
                     MKEDELAKFKQMMGKQQRKTG"
     gene            complement(4509..5051)
                     /locus_tag="VFX94_10355"
     CDS             complement(4509..5051)
                     /locus_tag="VFX94_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5768625.1"
                     /translation="MSERILVVDDDPGVSEVIGLLLEREGYAVQRAGSVKQGIARVEA
                     SELDLVVTDLKLPDGTGLDVIAGVRARRPRLPIIMITSYSSMESAIDALRAGANDYVI
                     KPFNNDEFLRAVARGLNDRRVSRGARPLAIEEYIREVVERFQDSYSETELARMLGIGR
                     KALWMRRRQWGLKRQRKSVS"
     gene            complement(5048..>5442)
                     /locus_tag="VFX94_10360"
     CDS             complement(5048..>5442)
                     /locus_tag="VFX94_10360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5768626.1"
                     /translation="LRHSASGERVRVEYRGAPGVKVRARAGELEQVVFNIFLNAAQAM
                     PAGGSIEAHAHAGAHEIVLSIRDSGAGIAAEHLARVFEPFFTTKPPGEGTGLGLAISY
                     EIVHELGGAIRAGNDPRGGACFQLTLPR"
BASE COUNT          889 a         1827 c         1877 g          849 t
ORIGIN      
        1 cgattacctt catcaccttc ctcggcgcct tctacaccat cgtgatcaac gtgctgggcg
       61 gcgcgcgctc gatcgacgtg cgctacttcc aggccgccag ctcgatgggc tcgtcgcgct
      121 gggacatctt ccgccgcatc atcctgcccg gcacgctgcc gtcgatcgtg gtcggctcgg
      181 cggtcggcat gggcatcacc tgggaagtgg tggtggcggc cgagatgatc tcgggcggcg
      241 gcagccagca ggccggcgtc ggcggcggcc tgggcttctt catctggaac tcctacgtcg
      301 gcggctccta cgagcagatc gtggtcggca tgatctcgat cggcatcgcc ggcttcatct
      361 ccagcgagct gctgcgctgg ggcggacgat acgccacccc atggctgaaa ctgcggtaag
      421 gccaaagatc gttccaggcg agatcaccgt cgacgcggtg accaagtcct atggcgcgct
      481 gcagttccgc aaggacgtgg tgcaggagtg ctcgttcacg atcgagcgcg ccaagctcac
      541 cgtgatggtc ggcccgtcgg gctgcggcaa gagcacgctg atccgcctgc tggccggctt
      601 cgagcagccg accgcgggcc gcatcctgct cgatggcgcg ccggtcgccg cgccgagccg
      661 cgaccggctg gtggtgttcc aggagacggc gctgttcccc tggatgacga cctggaacaa
      721 catcttgtac ggcccgcgcg cgcgcggcga gctcacgcgc gagacgcagc agttcggcga
      781 gttcctgctc gccaaggtcg ggctgcagga attccgcgac aagtaccccg gccagctctc
      841 gggcggcatg cagcgccgcg ccgagctcgc gcgagcgatg atcaacaacc cgaaggtcat
      901 gatcctcgat gagccgtttc gcggcctcga cgccatgacc aaggagctga tgtgggagta
      961 ctactcgcgc ctgtacgagg agaaccggcg caccaacttt ttcgtcacca cggacatcga
     1021 cgaggcggtg ttcctcgccg accgcatcct ggtgatgtcg aacatcccga cccaggtgcg
     1081 cgccacgctc gaggtcgacc tgccgcgccc gcgcaagctg ctcgacatct tcgagagcga
     1141 ccgcgccaac gacatcaaga agcaggcgct ctcgatcctg cacgcggagg cgatgcgctc
     1201 gttctcgcgc ggttccaagg cggccgcgga cttcgttgag gcgtacctga agcgcgcgtc
     1261 gcgcgcgtga tggagatcgt gctgacgaag ccggcggtgg tggcgcttgc ggtcctcggc
     1321 gcgctgcttt gcaccggcgc gtccttcctg cagatgcgcg gctacgtcag tgagaaggcc
     1381 ggcaaggcgt tgaactgggc gggctacggc gtcatgggcg cgagcattct gctgttcgtg
     1441 ctcgccggcc tgcgcggggt ccagccctga tcatcgtcag aggggcgcgg cgcgcggccg
     1501 gcggcgctcg gcgtcgtaga acggcagccg cgcaacgcgc gccaaccgcg cgccggcgcg
     1561 aacgcccgcg ccctccgcga tgcccggagc gacgaagccc agtgcgatcc gcttggcaag
     1621 cgtcggcgaa tagcattcgc tagtgagctg tgccaccggg tattcggcgt cgacggcctc
     1681 gtcgaaaatt tccaggccca cgaagcgccg cgtcatcggg aatgcctgcc ctggtgcgag
     1741 ccgttccaga ccgagctcgc ggggattggc gcggccgtcg acttcgcggt cgaaaagcac
     1801 gtaaccggct tcgatgcgca ggctgttcgc cgctgcccat gtgcaatcac gcaagtcgtc
     1861 gctgcgtccg gcatcgagga gccgtcggcc cagcgcggcg gcatctgcgt cgggaaccag
     1921 aagctcgtag ccgagctcgc cgctataacc gagacggccg accaggagcg gcgtctggat
     1981 gaagcggaaa taattgagcg cgcgcacggc ctgggcgccg atgaggcgcg ccaggatgcg
     2041 gccgcttgcc gggccttgga gcgcgaggat ggcgtattcg ccggagcggt cgcggccgcc
     2101 atagcgcgcg cgcttgccgg aaaacaacca gtaacgccgc tcgtccaggc gccagaccgt
     2161 ggcgtcgatc agcacggcgc cgtcctgctt gagcagcaac gtataggcaa tctgccctgg
     2221 gacgagcgca tccagctcgc gcgtctgcag cttctgcagc tcggccttgt cgtcgaactc
     2281 gtagagtccc atgaaggaga agtcgaacag ccccgcgctg cggcgcgtcg cgagatgttc
     2341 ttcgagagca ctcacaggct tcgcgccagg agcagcgcgc cggtgatcac gcagagcgca
     2401 ccggcgagcc ggcgcagcgc gagcgcgctc gcccggtggg cgaggcgaac gccgacgagc
     2461 acgccggcga gctcgaacac ggtgaccaag acagcggtgt cccagtcgat gaagccgttg
     2521 cgcaggttct cgagcgagcc gctcgtcgcg gcgacaatct gcagcgcctg gctggtggcc
     2581 acggcggtca gcggcacata gcgcaggatg accatgagcg gcacggagaa gagcggtccg
     2641 cccgcgccgg agaacccgga gccgaagccg gaagcggcgc ctacgccggc gagcagccac
     2701 gcctctccgc gccgccggcc gtcgcgcggc tgcgccgaag cgcgctgcgg cacgaggacg
     2761 tagacaccgg cgagcacgat gaggagggcg atcgcgatcg cgagcgggcg cggatccagg
     2821 cgcgcggcga gcgcagcgcc gaggaagccg caaacgagcg ccgcgccgca caccggagcc
     2881 gcgatgcgcc agtcgatgct gccgcggcgc tggtagagcc aggtgccgag aatgccggtg
     2941 aagagaaagc tgaagagcga cgtggccgcg gcctggtgga tcgtcaggcc ggcgaacagc
     3001 gcaagatagg acaccatgag cacgccgccg acgcccaccg ttccaatgag caggcccacg
     3061 aacgcggcaa ccgccgcgag aaggaggctg gcggtcagcc ggcggcgaag gcgacgagct
     3121 cgctcgtgag caggaccgcg agcttcgtca tgagcgcgta gcccacgccg gtgtcggccg
     3181 ccatgagccg ggtgagcgcg ccgccatcga tggcgatcac ctcgcacggc gtaaggcagt
     3241 acgcggtggc gatgcggcgc gcgtggccct ccaccagcgg tgcccagccg aacacgtcgc
     3301 cgcggcggat gatgtcgccg gccgcggtgt cgcgcttacc gagcccgagg gtgaagcgca
     3361 ccatgccgtc ggcgagcacg tagaagtcgt ccacccggtc gccgacggta tagatgcgcg
     3421 tgtcctgcga gaacgagcgc gcgcgcccga gcgccgcgac gcgctcccgc tgcgctctgt
     3481 cgaggccgga aaaaaagcgc gcctgctcca gcacgcgcgc cggctcgagg gagagcgcgc
     3541 cgctcacccg gtttttcgct gctgcttgcc catcatctgc ttgaacttgg cgagctcgtc
     3601 ttccttcatg aacacggtgt gctcggggcc gggatagcgg cgcgcgcgca catcgtcgcg
     3661 gtagtccgac agccccttct cgaggatcgg cacgaggtcc acgtagatct tggagtggcg
     3721 cggcacgtgc gcttcgtaca ggtggaacag gtccgagccg atgatgtgca cgccatgcgc
     3781 gcggttgccg gcgccgagcg agatcaccgg cacgggcagc gtctcggcga ggtattcgca
     3841 gacctccgaa gtggtgacct cgcacatgat cgaaaagcag ccggcgtcga ccatcgccca
     3901 ggcgtcgtcg atcagctcct tggcgcgctc ggcggtcttg ccctgggcga agaagccgcc
     3961 gagctgcggc atgcgcatcg gcgtgatgcc gatgtggccc tgcaccggga tgccggcctt
     4021 gacgatcgcc tcgatgtact tggcgtggtg ctggttgccc tcgcacttca tcacctcggc
     4081 gccgccctgc gagacgtact tcgcggcgtt gtcgaccgcc tgctgcgccg agaggtggaa
     4141 gctcatgtag ggcatgtcga ccatgcgcag tccgaatctg gatccgcgct tcaccgcctg
     4201 cgccatgaac atcacttcct cgaaggacac cgaggtcgtg gtttcgtgcc cgaagagcac
     4261 catgccgccg gtatcggaca cgcacaggat gtcaattccg acgcggtcgg cgatgatcgc
     4321 ggtctcgtag tcgtacaccg cgagctgaac gatcggctcg cccttcttca tcttttccac
     4381 cagcatcggg atagtgacct tcttgcgcgc gggctgcgcg ggcttggcgt cggccatggc
     4441 tgcgattcct cctctaagga atgattgaga taacaagagc gcggccggaa tgcaagatgc
     4501 ctgtttccct atgaaacgct tttccgctgt ctcttcaaac cccattggcg gcggcgcatc
     4561 cagagggcct tgcggccgat gccgagcatg cgcgcgagct cggtttcgct gtaggaatcc
     4621 tggaagcgct cgaccacctc gcggatgtac tcttcgatgg cgagcggccg cgcgccgcgc
     4681 gatacgcgcc ggtcgttcag cccgcgcgcc accgcgcgca ggaattcatc attgttgaat
     4741 ggcttgatca cgtagtcgtt ggcgccggcg cggagcgcgt cgatggcgct ctccatggac
     4801 gagtagctcg tgatcatgat gatcggcagg cgcggccggc gcgcgcgcac gccggcgatc
     4861 acgtcgagcc ccgtgccgtc gggaagcttc aggtccgtga cgaccaggtc gagctcggaa
     4921 gcctccactc gcgcaatgcc ctgctttacg ctgcccgcac gctgcacggc gtagccttcg
     4981 cgctcgagca ggaggccgat gacctcggac acccccggat cgtcgtcgac gacgaggatg
     5041 cgctcgctca tcgcggcagt gtgagctgga agcacgcgcc gccgcgcggg tcgttgccgg
     5101 cgcggatcgc cccgcccagc tcgtgaacga tctcgtagct gatggcgagg ccgagcccgg
     5161 tgccttcgcc gggcggtttg gtggtaaaga acggttcgaa cacgcgggca agatgctcgg
     5221 cggcgatgcc ggcgccgctg tcgcggatcg aaagcacgat ctcgtgcgcg cctgcgtgcg
     5281 cgtgcgcctc gatgctcccg cccgccggca tcgcctgcgc cgcgttgaga aaaatattga
     5341 acaccacctg ctcaagctcg ccggcgcgcg cccgcacctt aacgcctggc gcgccgcggt
     5401 attccacgcg cacgcgttcg cccgaggcgc tgtggcgcag ga
//
LOCUS       DASUNT010000514         3254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1033607, whole genome shotgun sequence.
ACCESSION   DASUNT010000514 DASUNT010000000
VERSION     DASUNT010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033607"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFX94_10365"
     CDS             <1..378
                     /locus_tag="VFX94_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768627.1"
                     /translation="TVNILGDPDNGVIAGGYIGSFLLSGAYLAVSCMTSAMTRNQVVA
                     FILAVVICLFLVLAGFNPVTDLLTRWASPGFVDTVAAFSVVTHFDAFQRGVVDSRDLL
                     FFLSVIGFALFATGVIIRGHRSG"
     gene            381..2213
                     /locus_tag="VFX94_10370"
     CDS             381..2213
                     /locus_tag="VFX94_10370"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HEX5768628.1"
                     /translation="MKKYEHIVYSAIGLVALFLLLVAVNFLVSRAPLRLDLTQGDLYT
                     LSPGTRKILANLQSPVRVKLYASQGESVPVQLRSFTQRVEDMVREFKSVAGSNLIVER
                     YNPKPDSEEEDAAQLDGIEPQQLFTGEQFYLGLAVTQLERKQTIPTISPQRERLLEYD
                     LVRAIARVGSTERPKIGLMAGLPVLGERFNPFTRQASEPWVLANELKREFDVKEVPLT
                     TKEIDKDLNVILVIHPRDVQPATEYALDQFVMRGGKLIAFVDPYAYFDQMPTMPGMPQ
                     MPSGSNLPTLFKAWGVTMDPGKVVADVVFGSAGGARYTPTVLSLNRTAFSRDDVVTGQ
                     IETLLYAFGGAFEIKNTDLKVVELVKSSPNSMLVDSMGAAKSGDEATRGFKPSGTAMP
                     LAVRLTGKFKTAFPEGLKEDEKKPAAAGTPALRESPENSVIVVGDVDLLADGAAVDVQ
                     EVFGRKIVVPSNGNLAFALGMVEQFAAGDDLISLRSRAAAFRPLTVVRELEADAQKEY
                     FGRIQALEEEKQKTQARLQELQKAQGGAGKSGQILSAEQQAELERFKKTYIETNLALK
                     ELRKNLRQDAEALVFWTKVANIALMPLLVALLGLLVALFRRRYA"
     gene            2210..3235
                     /locus_tag="VFX94_10375"
     CDS             2210..3235
                     /locus_tag="VFX94_10375"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEX5768629.1"
                     /translation="MNARVVAILVVLLVVLGGGALLYQQQERSRRPENVGALGRTLFK
                     DLKAADIAALKVLEPNATLTVQRKEERWTIAERADFPADLSKVREFVLKVIGLKVGQS
                     EPLGDKDRSRLNLDASGTRVEFLGADGKPLGAFIVGKKHFKREVENPDKAIPDGRFVV
                     LPGEAGTAYLVADPLTQATVKTSEWIERSSFQVEKVKTLEVRYPGGGGYRIERAADNA
                     DWKLTGAKPGEKLDIPRANAASYSLQMLELADVAPKEARDTGLDKPISVDATTLEGAS
                     YAIKVGKLEGDNYYVSFTASGVKPEAKDAERVKIQSQHVLLIPKPKLEDTLKQRADLL
                     EKKEDKK"
BASE COUNT          589 a         1097 c         1048 g          520 t
ORIGIN      
        1 acggtcaaca tcctgggcga cccggacaac ggcgtcatcg ccggcggcta catcggaagc
       61 ttcctgctct cgggcgccta cctcgccgtg agctgcatga cttccgcgat gacgcgcaac
      121 caggtggtgg cgttcatcct cgcggtcgtg atctgcctgt tcctcgtgct cgccgggttc
      181 aatccggtca ccgacctcct gacgcgctgg gcgagccccg ggttcgtcga tacggtggcg
      241 gctttttcgg tggtgacgca cttcgacgcc ttccagcgcg gggtggtgga cagccgcgat
      301 ctcttgttct ttctctccgt catcgggttc gcgctcttcg ccaccggcgt catcatccgc
      361 ggccaccgtt ccggctagcc atgaagaaat acgagcacat cgtctattcg gccatcggtc
      421 tcgtcgcgct tttcctgctc ctcgtcgcgg tgaacttcct cgtgtcgcgc gcgccgctgc
      481 gcctggacct tacgcagggc gacctttaca cgctctcccc gggcacccgc aagatcctgg
      541 cgaacctgca gtcccccgtg agagtgaagc tttacgcctc gcaaggcgag agcgtgccgg
      601 tgcagctgcg cagcttcacg cagcgcgtcg aggacatggt gcgcgagttc aagagcgtcg
      661 cggggtcgaa cctgatcgtc gagcgctaca acccgaagcc cgattccgag gaggaggacg
      721 cggcccagct cgacggcatc gagccgcagc agctcttcac cggcgagcag ttctacctcg
      781 gcctggccgt cacgcagctc gagcgcaagc agacgattcc caccatttcg ccgcaacgcg
      841 agcgcctgct cgaatacgac ctcgtgcgcg cgatcgcccg ggtgggctcc accgagcggc
      901 caaagatcgg cctcatggcc gggctgccgg tgctcggcga gcgcttcaat cccttcaccc
      961 gccaggcctc ggagccctgg gtgctcgcga acgagctcaa gcgcgagttc gacgtcaagg
     1021 aggtgccgct caccacgaag gagatcgaca aggacctcaa cgtcatcctc gtgatccacc
     1081 cgcgcgacgt ccagcccgcg acggaatacg cgctcgatca attcgtgatg cgcggcggga
     1141 aactcatcgc gttcgtggat ccctacgcct acttcgacca gatgcctacc atgcccggta
     1201 tgccgcaaat gccgtcggga tcgaacctgc ccacgctctt caaggcctgg ggagtcacga
     1261 tggacccggg caaggtggtg gccgacgtgg tgttcggctc cgccggcggc gcgcgctaca
     1321 cgccgacggt gctttcgcta aaccgcaccg cgttcagccg cgacgacgtc gtcacgggcc
     1381 agatcgagac gctgctgtac gcattcggcg gcgccttcga gatcaagaac accgatctga
     1441 aagtcgtcga gctggtgaag agctctccca attcgatgct cgtcgacagc atgggcgccg
     1501 ccaagtccgg cgacgaggcg acgcgcggct tcaagccctc cggtaccgcc atgccgctcg
     1561 cggtgcggct caccggcaag ttcaagaccg cttttcccga agggctgaag gaggacgaga
     1621 agaaaccggc tgcggccggc accccggcgc tgcgcgagtc gccggagaac tcggtgatcg
     1681 tggtgggcga cgtcgatctt ctcgccgacg gcgcggcggt ggacgtgcag gaggttttcg
     1741 ggcgcaagat cgtcgtgccg tcgaacggca acctcgcctt cgcgctcggc atggtggagc
     1801 agttcgccgc gggcgacgac ctgatctcgc tcagaagccg cgcggcggcg ttccgcccgc
     1861 ttaccgtagt gcgcgagctc gaggccgacg cgcagaagga gtatttcggc cgcatccagg
     1921 cgctcgaaga ggagaagcag aagacccagg cgcggctgca ggagctgcag aaggcgcagg
     1981 gcggcgcggg caagtcgggc cagatcctca gcgccgagca gcaggccgag ctcgagcgct
     2041 tcaagaagac ctacatcgag accaacctcg cgctcaagga gctgcgcaag aacctgcgcc
     2101 aggacgccga ggcgctcgtg ttctggacaa aggtggccaa tatcgcgctc atgccgctcc
     2161 ttgttgcgct gctcgggctg ctcgtcgcgc tgttccgcag aaggtacgca tgaacgcccg
     2221 cgtcgtcgcc attctggtcg tcctcctcgt tgtccttggg ggcggagctc tgctctacca
     2281 gcagcaggag cgctcgcgcc ggccggaaaa cgtcggcgct ctggggcgca cgctgttcaa
     2341 ggacctgaag gccgccgaca tcgcggcgct caaggtgctc gagccgaacg ccacgcttac
     2401 cgtgcagcgc aaagaagagc gttggacgat cgccgagcgc gccgattttc ccgccgactt
     2461 gtccaaggtg cgcgagttcg tgctcaaggt gatcggcctg aaggtcggac agagcgagcc
     2521 gctcggcgat aaggaccgct cgcgcctgaa tctcgacgcc tcggggacca gagtggaatt
     2581 cctcggcgcc gacggcaagc cgctcggcgc gttcatcgtc ggcaagaagc acttcaagcg
     2641 cgaggtggag aaccccgaca aggcgatccc ggacgggcgc ttcgtcgtac tgcccggcga
     2701 ggccgggacc gcctacctcg tcgccgaccc gctcacccag gccaccgtca agacgtccga
     2761 atggatcgag cgcagctcct tccaggtgga aaaagtgaag acgctggaag tgcgctaccc
     2821 cggcggcggc ggctaccgca tcgagcgcgc ggccgacaac gccgactgga aactcaccgg
     2881 cgctaagccc ggcgagaagc tcgacatccc gcgggcgaat gcagcgtcgt actcgctgca
     2941 aatgctcgag cttgccgacg tcgcgcccaa ggaggcgagg gacaccggcc tcgacaagcc
     3001 gatcagcgtg gacgcaacca cgctcgaggg cgcgtcgtac gcgatcaagg tcggcaagct
     3061 cgagggcgac aactactatg tgagcttcac cgcctcgggc gtgaagcccg aagcgaaaga
     3121 cgccgagcgc gtgaagatcc agtcgcagca cgtcctcctc atcccgaagc ccaagctcga
     3181 ggacaccctc aagcagcgcg ccgacctgct cgaaaagaag gaagacaaga agtagtggcg
     3241 ttggacccgc atct
//
LOCUS       DASUNT010000515         3053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_595249, whole genome shotgun sequence.
ACCESSION   DASUNT010000515 DASUNT010000000
VERSION     DASUNT010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595249"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFX94_10380"
     CDS             complement(<1..282)
                     /locus_tag="VFX94_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX5768630.1"
                     /translation="MPEPKTIEVTLLGRSYRVACEDNEREALMQAVAYLDAKMNEIRK
                     AGRVMGAERIAVMAALNVAHELLNMKLGAGFDLGQAKRRLVAIESQIDAA"
     gene            complement(287..547)
                     /locus_tag="VFX94_10385"
     CDS             complement(287..547)
                     /locus_tag="VFX94_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768631.1"
                     /translation="MNYMQNATLLVDAFRLLPHSAGCVDNEFTSLEAKIARFVSLCER
                     LRAENVELRQQLAAARSDAKRLHEKIDGAKARLEGLLARLPG"
     gene            complement(544..1380)
                     /locus_tag="VFX94_10390"
     CDS             complement(544..1380)
                     /locus_tag="VFX94_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011325252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX5768632.1"
                     /translation="MRWAGMVLALVVCSAAADVSVKDDYGNLLRLPSPAARIVSLAPH
                     LTELVYAAGAGERMVGAVEYSDFPPAARALARVGNDAHISLESVLALRPDLIVAWPNP
                     GSVRAINRLAELGIPVFRSEPRELEHIATTLERLGTLAGTNAPAMREARLFREKKAEL
                     EKRYAGRPKVRVFYQVWDRPLVTVNGDHVISKVIALCGGENVFAALPLIAPEVDAEAV
                     LRAAPEVILTSRPGAQPPRGLPGSRLAAVPADLLQRHTPRLLEGAEQVCRALEDARSA
                     RS"
     regulatory      1389..1554
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1591..>3053
                     /locus_tag="VFX94_10395"
     CDS             1591..>3053
                     /locus_tag="VFX94_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5768633.1"
                     /translation="MRFTLAGLVFCTSCLAQESSVVITAPRFPEEIRRLPASVTVITE
                     EDIRRSAARTLPELLSSEVGITMKDFYGNNAAYTSIDLRGFGATGSQNTLILLDGRRL
                     NDFDLSGVQWPAIPLASIERVEIVRGAGGVLYGDGATAGIINIVTRSPLTPGKSLELL
                     GRVASYDTREGQLYASYGTPRFGINGSVYGYESDGYRENNRNEQQNNTLNLRWALGDG
                     ALDLRVATDSQDIRLPGGRFVQPSIGLDEYTTDRRGAATPLDYASRDGTRGRLSFMQH
                     LGEAEFSISLDTRDKDQRSYFDQAGFPTYRADDLELRSLTPRVKLPLGRQTFVFGVDL
                     HRWRYRSSRSDRPENIERPTNRVSVDQDTAGVYVLDTIALTPATVAVAGWRSERAKYD
                     ATDEVDPGAPGCFFCSAAPAVQETQNEHAWELGLRHAFGPQWSAFARAGRAFRFANAE
                     EIYESDVFFAPQFQILRPQHSRTHEAGAEWRGVRHAL"
BASE COUNT          512 a         1068 c          985 g          488 t
ORIGIN      
        1 cgccgcgtcg atctgcgatt cgatggcgac caggcggcgc ttcgcctggc cgagatcgaa
       61 gcccgcgccg agcttcatgt taagcagttc gtgcgccacg ttcagcgccg ccatcaccgc
      121 gatgcgctcc gcgcccatca ccctgcccgc cttgcggatc tcgttcatct tggcatcgag
      181 ataagcgacc gcctgcatca gcgcttcgcg ctcattgtcc tcgcacgcca cgcgatagct
      241 gcggccgagg agcgtgactt cgatcgtttt tggctcgggc acggcgtcag cccggcaggc
      301 gcgcaagcag gccctcgagg cgtgccttcg cgccgtcgat tttctcgtgc aggcgcttcg
      361 cgtcgctgcg cgccgcggcg agctgctggc gcagctcgac gttctccgcg cgcagccgct
      421 cgcaaagcga tacgaaccgc gcaatcttgg cttccagcga agtgaactcg ttgtccacgc
      481 agccggcact atgtggcaaa agcctaaacg cgtcaactaa taaggtcgcg ttctgcatgt
      541 agttcatgat cttgccgagc gcgcgtcctc cagcgcccgg cagacctgct cggcgccttc
      601 cagcagccgg ggcgtatggc gctggaggag gtccgccggc accgcggcga ggcggctccc
      661 gggcagtccc cttggaggct gcgccccagg gcggctcgtc aagatgacct cgggagcagc
      721 gcgcaagacc gcctcggcgt ccacttcagg cgcgataagc ggcagcgcag caaagacgtt
      781 ctcgccgccg caaagtgcga tcaccttgct gatcacgtga tcgccgttca cggtgacgag
      841 cggccggtcc cacacctggt agaacacgcg cactttcggc cggccggcgt agcgcttctc
      901 gagctcggct ttcttctctc gaaacaggcg cgcttcccgc atggcgggtg cgttggtgcc
      961 tgccagcgtg cccagccgtt ccagcgtcgt cgcgatgtgc tcgagctcgc gcggttcgga
     1021 acgaaatacc gggatgccga gctccgccag ccggttgatt gcgcgcacgc tccccggatt
     1081 gggccacgcg acgatgagat cgggccggag cgcgagcacg gattcgaggc ttatatgcgc
     1141 gtcgtttccc actcgcgcca gggcgcgcgc cgcgggcgga aagtcgctgt actcgacggc
     1201 tcccaccatg cgctcgcccg cgcctgcggc atagacgagc tcggtgaggt gcggcgcgag
     1261 actcacgatg cgcgcggcgg gcgaaggcag gcgcaagagg ttgccgtaat cgtccttgac
     1321 cgagacatcc gcggccgcgc tgcagacaac aagggccaac accatgcccg cccatcgcat
     1381 gcggctatca tatcggcacg ctcccgggtg cacgggaaag aggtgagaag cctccactgc
     1441 ccccgcaacg gtaagcgaga gagacgctct tgaagtgcca ctgcgcgtag cgcgggaagg
     1501 cgagagcgtt atgcctcgcg agtccggaga ccggcctgga agcgcctttc aaccatttca
     1561 ggcgtcgggg aagacgccag ggaggtttcg atgcgcttca cccttgcagg tctggttttc
     1621 tgcacgagtt gtctcgcgca ggaaagctcg gtcgtcatca cggcgccgcg ctttcccgaa
     1681 gagatacgcc gcctgccggc gagcgtcacg gtcatcaccg aggaggacat ccggcgcagc
     1741 gccgcccgca cgctgcccga gctcctgtcg agcgaggtcg gcatcacgat gaaggatttc
     1801 tacggcaaca acgccgccta cacctcgatc gacctgcgcg ggttcggcgc gaccggctcg
     1861 cagaacacgc tcatcctgct cgacggacgg cggctgaacg acttcgacct gtcgggggtg
     1921 caatggccgg cgatcccgct cgcgtccatc gagcgcgtcg agatcgtacg cggcgccggc
     1981 ggcgtgctct acggcgacgg cgcgaccgcg ggaatcatca acatcgtcac gcgctccccc
     2041 cttactcccg ggaagagcct cgagctcctc ggccgtgtcg cgagctacga cacgcgcgaa
     2101 ggccagctct acgcgagcta cggtaccccg cgcttcggca tcaacggctc ggtatacggc
     2161 tacgagtccg acggctaccg tgagaacaat cgcaacgagc agcagaacaa cacgctcaac
     2221 ttgcgctggg cgctgggcga cggggcgctc gaccttcgcg tggccaccga cagccaggac
     2281 atccggctgc ccggaggccg cttcgtgcag ccctcgatcg ggctcgacga gtacacgacc
     2341 gaccgccgcg gcgcagcgac gccgctcgat tacgcaagcc gcgacgggac gcgcggtcgc
     2401 ctgtctttca tgcagcacct cggcgaagcc gagttctcga taagcctgga tacgcgcgac
     2461 aaggaccagc gctcctattt cgaccaggca ggcttcccga cctaccgggc ggacgacctg
     2521 gagctgaggt cgctcactcc ccgggtgaag ctgcccttgg gccggcaaac tttcgttttc
     2581 ggagtcgacc tgcaccggtg gcgctaccgc tcctcgcgca gcgatcggcc ggagaacatc
     2641 gagcgcccga ccaaccgggt gtccgtggac caggacaccg ccggggtcta cgtgctcgac
     2701 accatcgctc tcaccccggc caccgttgcc gtagccggct ggcgtagcga acgggcgaaa
     2761 tacgacgcga ccgacgaagt cgatcccggc gcgcccggct gctttttctg cagcgcagcc
     2821 cccgccgtgc aggaaacgca gaacgagcac gcgtgggaac tgggcctgcg gcatgccttc
     2881 ggcccccagt ggtcggcgtt cgcgcgcgcc ggccgcgcgt tccgcttcgc gaacgccgaa
     2941 gagatctacg agagcgacgt cttcttcgcg ccgcaattcc agatcctgcg cccgcagcat
     3001 tcgcgcacgc acgaagccgg cgcggaatgg cgcggcgttc gccatgccct gag
//
LOCUS       DASUNT010000516         2797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_421573, whole genome shotgun sequence.
ACCESSION   DASUNT010000516 DASUNT010000000
VERSION     DASUNT010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421573"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..220
                     /gene="hfq"
                     /locus_tag="VFX94_10400"
     CDS             2..220
                     /gene="hfq"
                     /locus_tag="VFX94_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX5768634.1"
                     /translation="MLQDPFLNTLRKEHVPVSIYLVNGIKLQGQIESFDQYVVLLRNS
                     VTQMVYKHAISTVVPSRPISLGPDKDSE"
     gene            240..1406
                     /gene="hflX"
                     /locus_tag="VFX94_10405"
     CDS             240..1406
                     /gene="hflX"
                     /locus_tag="VFX94_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX5768635.1"
                     /translation="MERSAAAADPQAAVIVCLDFGEPHYEESVEEVVRLVQSAGVRRY
                     RVVKGRRTRPDARLYAGAGKVEEIGRAAEQINAMYVVFNHALSPAQERNVERAIVRRV
                     MDRAELILEIFAQRAQTAEGKLQVELAQLEHLSTRLVRGWTHLERQRGGLGKTGGPGE
                     TQLELDRRYIANRVRVLKKKLETLKRQRGVQRRARARGEVLSVSIVGYTNAGKSTLFN
                     ALSGAKAYEADQLFATLDTTTRRIWLPDAGNVVVSDTVGFIRDLPHGLVAAFRATLEE
                     TVHADLLLHVVDASSSTREEQVQAVDKVLAEIPAGAAPRLAVWNKIDATWHNPAKAGL
                     EPAVERDQYGRISRVFLSARTGVGMELLRGAIAEFAKARAAQRSATALPLQLTR"
     gene            1407..2597
                     /gene="hflK"
                     /locus_tag="VFX94_10410"
     CDS             1407..2597
                     /gene="hflK"
                     /locus_tag="VFX94_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEX5768636.1"
                     /translation="MSLNDPNWGRGSRGPQGPGGGNQGPPDLDELWRNFNRRLSELFG
                     RRRGGPDEPPRPPSGRGLGGGAGVLIALVLVIWLASGFYIVVEGQRGVVLTFGRYSQE
                     TMPGLRWRLPWPIQSNEIVNLAQVRTLEVGYRNNVRTKVLKESLMLTDDENIVDLQFA
                     VQYLVKDARDFVFNVRRPDESAMQIAETAMREVIGKSRMDAILYETQIDVANRARDLM
                     QVIHDRYGTGITISTVTIQNAQPPEQVQAAFDDAVKAGQDRERQKNEGQAYANDVIPR
                     ARGTAARLAEEAQGYRQRVIANAEGDASRFKQVLAEYAKAPAVTRERIYIETMQAVLT
                     STSKIMMDYRGSGNLLYLPLEKIMQSAGAQEGAPQTAAPPAPPAEAGPRSRETLRNRE
                     RGDR"
     gene            2597..>2797
                     /locus_tag="VFX94_10415"
     CDS             2597..>2797
                     /locus_tag="VFX94_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX5768637.1"
                     /translation="MKALGPVVAILAVLALIASSAMFTVDQRQNAIVFQLGEVKEVVN
                     RSGLHFKWPLLQNVRQFDMRILT"
BASE COUNT          509 a          928 c          950 g          410 t
ORIGIN      
        1 gttgctacaa gacccgtttt tgaacaccct gcgcaaagag cacgttccgg tctcgatcta
       61 cctggtgaac gggatcaagc tgcagggcca gatcgagtcg ttcgaccagt acgtcgtgct
      121 gctgcgcaac tccgtcaccc agatggtgta caagcacgcc atctccactg tcgtgccttc
      181 gcgaccgatc agcctgggtc ccgacaagga ctcggaataa ggctgatttt cgtaccctga
      241 tcgaaagatc cgccgcagcc gccgatcccc aggcggcggt catcgtctgc ctcgacttcg
      301 gcgagcctca ttacgaggag tcggtggagg aagtggtgcg tctggtgcaa agcgcgggcg
      361 tgcgccgcta ccgggtagtc aagggaagaa ggacccggcc cgatgcccgg ctctacgccg
      421 gtgccggcaa ggtggaggaa atcggccgtg ccgccgagca gatcaacgcg atgtacgtgg
      481 tgttcaacca cgcgctctcg ccggcgcagg agcgcaacgt cgagcgcgcg atcgtgcgcc
      541 gcgtgatgga ccgcgccgag ctgattctcg aaatcttcgc gcagcgcgcg cagaccgccg
      601 agggcaagct gcaggtcgag cttgcgcagc tcgagcatct ctcgactcgc ctcgtgcgcg
      661 gctggacgca cctcgagcgc cagcgtggcg gactgggcaa gaccggcggg ccgggcgaga
      721 cgcagctcga gctcgaccgg cgctacatcg ccaaccgggt ccgggtgctg aagaaaaagc
      781 tcgagaccct gaaaaggcaa agaggcgttc agcgccgtgc gcgcgcgcgc ggcgaggtgc
      841 tttcggtatc gatcgtgggc tacaccaacg cgggcaagtc gacgcttttc aatgcgctga
      901 gcggcgcgaa agcctatgag gccgatcagc ttttcgccac gctcgacacc acgacgcgcc
      961 gcatctggct cccggacgcc ggcaatgtag tggtctcgga cacggtgggc ttcatccgcg
     1021 acctgccgca cgggctggtc gccgcgtttc gcgcgacgct ggaggagacc gtgcatgccg
     1081 atctgctgct ccacgtcgtc gacgcctcgt ccagcacgcg cgaggagcag gtgcaggccg
     1141 tggacaaggt cctcgcggag atacctgcgg gcgcggcacc gcgcctcgcc gtctggaaca
     1201 agatcgacgc gacctggcac aaccccgcaa aggcagggct cgagcccgcg gtcgagcgcg
     1261 accagtatgg taggatttcc cgcgtattct tgagcgcgcg cacaggcgtc gggatggagt
     1321 tgttgcgcgg cgccatcgca gaattcgcaa aggcccgggc cgcgcagaga agcgctaccg
     1381 cactccccct gcaactcact agataaatgt ccttgaacga ccccaactgg ggacgcggct
     1441 cgcgcggccc gcagggcccg ggcggcggca accagggccc gccggacctc gacgagctgt
     1501 ggcgcaattt caaccgcagg ctctcggagc tcttcggccg ccgccgcggc gggcccgacg
     1561 agccgccgcg gccgccgagc gggcgcgggc tgggcggcgg cgccggcgtg ctgatcgcgc
     1621 tcgtgctcgt gatctggctc gcgagcggct tctacatcgt ggtggaaggc cagcgcggcg
     1681 tggtgctcac cttcgggcgc tattcgcagg agaccatgcc gggcttgcgc tggcgcctgc
     1741 cctggccgat ccagtcgaac gagatcgtga acctggcgca agtgcgcacg ctcgaagtgg
     1801 gctaccgcaa caacgtgcgc accaaggtgc tcaaggaatc gctcatgctc accgacgacg
     1861 agaacatcgt cgacctgcag ttcgcggtgc agtacctggt gaaggacgcg cgcgacttcg
     1921 tcttcaacgt gcgccggccc gacgagtcgg cgatgcagat cgccgagacg gcgatgcgcg
     1981 aggtgatcgg caagagtcgc atggacgcga tcctgtacga gacccagatc gacgttgcga
     2041 acagggcgcg cgacctgatg caggttatcc acgaccgcta cggcaccggc atcaccatca
     2101 gcacggtgac gatccagaac gcgcagccgc ccgagcaggt gcaggcggcc ttcgacgacg
     2161 cggtcaaggc gggccaggac cgcgagcggc aaaaaaacga aggccaggcc tatgccaacg
     2221 acgtcatccc gcgcgcgcgt ggcacggcgg cacgcctggc cgaggaggcc cagggctacc
     2281 gccagcgcgt gatcgccaac gccgaaggcg acgcctcgcg cttcaagcag gtgctcgccg
     2341 agtacgccaa ggcgccggcg gtgacgcgcg agcgcatcta catcgagacc atgcaggcgg
     2401 tcctcacctc gaccagcaag atcatgatgg actaccgcgg ctcggggaac ctgctctacc
     2461 tgccgctcga gaagatcatg cagagcgccg gcgcgcagga aggcgcgccg cagaccgcgg
     2521 ctccgccggc gccgccggcc gaggcggggc cgcgctcgcg cgagacattg cgcaaccgcg
     2581 aaaggggcga tcgctagtga aagcgctcgg gcccgtcgtc gccatcctcg ccgtgctcgc
     2641 gctgatcgcc tcgagcgcga tgtttaccgt ggatcagcgc cagaacgcga tcgtgttcca
     2701 gctgggcgag gtgaaggaag tcgtcaaccg ctcggggctg catttcaagt ggccgctgct
     2761 gcagaacgtg cgccagttcg acatgcgcat actgacc
//
LOCUS       DASUNT010000517         3094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_945794, whole genome shotgun sequence.
ACCESSION   DASUNT010000517 DASUNT010000000
VERSION     DASUNT010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945794"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1451)
                     /locus_tag="VFX94_10420"
     CDS             complement(<1..1451)
                     /locus_tag="VFX94_10420"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5768638.1"
                     /translation="MDALQLLGQGFATCFTPLNLSFIFLGVLIGQIIGALPGIGPAAG
                     MALLLPLTYGVPPVTAIMMLAGIMYGGQYGGTLTSVLVNVPGEAASVMTTIDGHQMAR
                     QGRAGAALSIAAIGSFLAGIAAVVAVVFLTPPLAELALKFNAPEYFLLALLGITATAS
                     LGTDSPTKALLAALLGLMIAMVGVDPIQGTPRLVFGQQSLIEGIDFLPVAIGIFGIAE
                     VLASLERSEHVAPMRTRLRDMWLTAKDWAESRMAILRGALIGFVVGVMPGAGPTIASM
                     LAYIAEKRLSRRPERFGRGAIDGVAAPESANNAASHAACVPMLALGIPGSASTAVLLA
                     ALILHGIRPGPGLMTEQSTLVWALIASMFIGNVVLLVLNLPLAPLFAALLRVRYAYLA
                     PGILAISLVGAYANSLGFTNMWIALAFGFVGWFMIKLDFPRAPLVLALVLAPLLESSL
                     RQSLLLSFGSLKIFVERPLAAALLACVVVSLLIP"
     gene            complement(1454..1906)
                     /locus_tag="VFX94_10425"
     CDS             complement(1454..1906)
                     /locus_tag="VFX94_10425"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX5768639.1"
                     /translation="MRPSDVVPALLMLALSAAIAVDTRALSFWADTTPGPAFLPVWLA
                     IAGAVLFVLRLAEARRGGGGPPVQWPERAAVARVALVFAALVAVPLVSPVVGLAPALA
                     LLVAFVLLFVLRQRLWPSLAAVGITLGLIYAVFVGWLGVPLPKGPLGL"
     gene            complement(1911..2876)
                     /locus_tag="VFX94_10430"
     CDS             complement(1911..2876)
                     /locus_tag="VFX94_10430"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5768640.1"
                     /translation="MVLKAVAVALGIALTPGAIAQMKAPKGPVEITVGSGPGGTPDVI
                     MRNIVKIMTEEKIVTIPVVVQNRPGGSHSNAYNYVLGKKGDENLLLTLASPVFTTPIM
                     QGTPSVVQQVVPIAGFIQSELVFLVQPNSPYKTLKDFVEAAKKEPKKLRIAGAASGGT
                     DHLATAQVEKAAGIRLTYIPYESGSKALASFLGGNVEATFATLEEGLPVFKENKARPL
                     AILSEKRRPEAAYKDVPTAKELGYDIVFGQFWGVAAPPETDPAIARWWEDKFRKAVAT
                     KAWQEGLKEKFQRSDFYGLEKAGEYFKREEAKFRSLMEDVGLAKK"
BASE COUNT          560 a         1035 c         1051 g          448 t
ORIGIN      
        1 gggattagca acgaaaccac tacgcacgcg agcagcgccg cggcgagcgg ccgctcgacg
       61 aagatcttca ggcttccgaa tgacagcagc agcgactggc gcagcgagct ctcgagcagc
      121 ggcgcgagca ccagcgcaag caccagcggc gcgcgcggga aatcgagctt gatcatgaac
      181 cagccgacga agccgaaggc gagcgcgatc cacatgttgg tgaagccgag ggaattggcg
      241 taagcgccga cgagcgagat ggcgaggatg ccgggcgcga gataggcgta gcgcacgcgc
      301 agcagcgcgg cgaacagcgg cgcgagcggc agattgagca cgagcagcac cacgttgccg
      361 atgaacatgc tggcgatgag cgcccacacc agcgtcgatt gctccgtcat caagcccggc
      421 cccgggcgga tgccgtgcag gatcagcgcg gcgagcagca ccgcggtgct cgccgagccc
      481 gggatgccga gcgcgagcat cggcacgcac gcggcgtgcg aggcagcgtt gttcgccgat
      541 tccggcgcag ccacgccgtc gatcgcaccg cggccgaagc gctccgggcg cctgctcaag
      601 cgcttttcgg cgatgtacgc cagcatggat gcgatggtgg gcccggcgcc gggcatgacg
      661 ccgacgacga atccgatcag agcgccgcgc aggatggcca tgcggctctc ggcccagtct
      721 ttcgcggtaa gccacatgtc gcgcagccgc gtgcgcatgg gcgcgacgtg ctccgagcgc
      781 tcgagcgagg cgagcacctc cgcgatgccg aaaatgccga tggcgaccgg caggaaatcg
      841 attccctcga tcaggctctg ctggccgaac accaggcgcg gcgtcccctg gatcgggtcc
      901 acgcccacca tggcgatcat caggccgagc agggcggcga gcagcgcctt ggtcggggag
      961 tcggtaccaa ggctcgcggt cgctgtgatg ccgaggagcg cgagcaggaa atactccggc
     1021 gcattgaact tgagggcgag ttcggcgagc ggtggagtca ggaaaaccac ggcgacgacc
     1081 gcagcgatac cggcaaggaa agagccgatc gccgcgatgc tcaaagcagc gccggcgcgc
     1141 ccctgtcgcg ccatctggtg accgtcgatc gtcgtcatga cgctcgccgc ttcgcccggc
     1201 acgttgacga gcaccgaggt aagcgtgccg ccgtactgac cgccgtacat gatgccggcg
     1261 agcatcatga tcgccgtgac cggcggaacg ccgtaggtga gcggaaggag cagcgccatg
     1321 cccgcggccg gcccgatccc gggcagggcg ccgatgatct gcccgataag gacaccgagg
     1381 aagatgaaag acaggttgag cggcgtgaag cacgtcgcga atccctgccc gaggagctgt
     1441 agcgcatcca ttgttacagt ccgagcgggc ccttgggcag cggcacgccc agccagccga
     1501 cgaaaactgc gtagatcagt cccagcgtaa tacccacggc agccaggctt ggccacagcc
     1561 gctggcgcag gacgaacaga agaacaaacg ccacgaggag cgcaagggcc ggcgcgaggc
     1621 ccacgacggg cgaaacgagc ggcacggcga cgagtgccgc aaagaccagc gcgacccgcg
     1681 cgacggcggc gcgctccggc cattgcaccg gcgggccccc gccgccgcgg cgcgcctccg
     1741 cgaggcgcag cacgaagagc accgcgcccg cgatggcgag ccacaccggc aggaatgccg
     1801 gccccggcgt cgtgtcggcc cagaagctca gcgcgcgcgt atcgaccgcg atcgcggccg
     1861 acagcgcgag catgagcaac gccgggacga cgtcgctcgg ccgcatgcgg ctacttcttc
     1921 gcgagcccga cgtcttccat cagcgagcgg aacttcgcct cttcacgctt gaagtactcg
     1981 ccggcctttt ccaggccgta gaaatcgctg cgctggaatt tctccttcag cccctcctgc
     2041 catgccttgg tcgccaccgc cttgcggaac ttgtcttccc accagcgcgc gatcgccggg
     2101 tcggtttccg ggggcgcggc gacgccccag aactggccga acacgatgtc gtagccgagc
     2161 tccttcgcgg tcggcacgtc cttgtacgcg gcctccggcc ggcgcttctc ggacaggatg
     2221 gcgagcgggc gcgccttgtt ctccttgaat accggcaggc cctcctcgag cgtcgcgaac
     2281 gtcgcttcga cgttgccgcc caggaaagaa gcgagcgcct tggaaccgct ctcgtagggg
     2341 atgtaggtga gacggatacc ggccgccttc tcgacctgcg cggtggcgag gtggtccgtg
     2401 ccgccggagg ccgcgccggc gatgcgcagc ttcttcggct ccttcttcgc cgcttcgacg
     2461 aaatccttga gggtcttgta gggcgagttc ggctgtacca ggaacacgag ctccgactgg
     2521 atgaacccgg cgatcggcac cacctgctga acgactgacg gcgtgccctg catgatcggc
     2581 gtcgtgaaca ccggcgaagc gagggtgagg agcaggttct cgtcgccttt cttgccgagc
     2641 acgtagttgt acgcgttcga atgcgatccg cccggacggt tctggaccac cactggaatg
     2701 gtgacgatct tctcctcggt catgatcttc acgatgttgc gcatgatgac gtcgggcgtg
     2761 ccgccaggcc ccgagccgac cgtgatctcc accggcccct tcggcgcctt catctgcgcg
     2821 atcgcgcctg gggtgagcgc gatgcccaac gctactgcaa cggccttcag caccatgatc
     2881 ctcctcctgt gtggacttca ttgctcgggc gagcgtagtc aagcattccg gtaagatcaa
     2941 gcagttgtct atcgcgacat gcaactcgag cgtgagatca agttcagcct gcccgcggcg
     3001 gcggcgcgcc gggtggcgcg gcaggtacgc cgcgcgggtc cgtggcgccg ccgcatggtt
     3061 ttcaacgcct actacgacac cgcgaacggg cggc
//
LOCUS       DASUNT010000518         2898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_158645, whole genome shotgun sequence.
ACCESSION   DASUNT010000518 DASUNT010000000
VERSION     DASUNT010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158645"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VFX94_10435"
     CDS             complement(<1..288)
                     /locus_tag="VFX94_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5768641.1"
                     /translation="MEVTRILVVDDDPAVRRMLVEYLSAHGYEVREAASGAAMRSELE
                     RATPTVVLLDIGLPGEDGLSLARYLRERHDVGIIMVTGAGALLAGAHESIDG"
     gene            395..571
                     /locus_tag="VFX94_10440"
     CDS             395..571
                     /locus_tag="VFX94_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEX5768642.1"
                     /translation="MKRIFRLAIDAFVSAREERARARLVSQLDERTLRDIGLEQEANH
                     ARERNRLAMRFGAY"
     gene            573..974
                     /locus_tag="VFX94_10445"
     CDS             573..974
                     /locus_tag="VFX94_10445"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HEX5768643.1"
                     /translation="MKRDIDLASEYVVHGSLGMTRGSVLRIEDGRDVALYVWEGGLWL
                     TQEGDRRDRYLGPGDCFRLERDGVTLAQAIEPSTVSLTAPQPESYAGRIALTKAGTGV
                     QVELSAGRKRDALGFWTRLFAPDARPTTASL"
     gene            971..1429
                     /locus_tag="VFX94_10450"
     CDS             971..1429
                     /locus_tag="VFX94_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5768644.1"
                     /translation="MTPRLRPLTRQDAARVQAFVRGLSSQSRYERFLAPIAELSPRQL
                     ERITAGTGLSLGAFDAAGRLVGLAEYARSGNAEAEFAIVIADEFQGQGLGAELVGALL
                     EHAKRSGILRLAGVTHTRNRAMRVLARRLGFGVQPDEDPDLVRMELALAA"
     gene            1426..1935
                     /locus_tag="VFX94_10455"
     CDS             1426..1935
                     /locus_tag="VFX94_10455"
                     /inference="COORDINATES: protein motif:HMM:NF015551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HEX5768645.1"
                     /translation="MNAAVLCAGATVFLWYAFGALPLHLSVVEELGLDPARASSWISI
                     VWMSSAVTSIAISLRYRQPIPITWTIPSLVLLGAVGGNFSAPELAGACLAAGVALLVL
                     GMAGAGADALERSFGGEMKFGALVALAVAATPFTVLGVGPALWALLCGLAASAAAERT
                     QLTAFWKAA"
     gene            1938..>2898
                     /locus_tag="VFX94_10460"
     CDS             1938..>2898
                     /locus_tag="VFX94_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5768646.1"
                     /translation="MGTNLYERAMASLWLQRGDARALAAAAVAEEPDAAAPRLLEAAL
                     LVTSRYVKDFEAAGWAYARIRGMPMTERERAHTSALEAAVDGDYERACLTYDRILLGT
                     PTDALALWTAQLMEYYLGNSAAMRERSGRVLARWSADTPGYHAVMSMHAFALQECGDY
                     GAAEEMALHALELEPRDLRAQHSLMHVYEMLGKPEEGMRWAARQAQQWCGDAAVAHHL
                     WWHTALFLTALERRHGALAVYDLRLQQDTIAGMIDASALLWRLHLEGVDVGARFATLA
                     GRWAAHAADAYCAFNDLHAMMAFAGAARWDLAQQLLAAQARRIE"
BASE COUNT          418 a         1023 c         1016 g          441 t
ORIGIN      
        1 gccatcgatg ctttcgtgag cgcccgcgag gagcgcgccc gcgcccgtga ccatgatgat
       61 tcccacgtcg tggcgctcgc ggaggtagcg cgcgagcgag aggccgtcct cgcccggcaa
      121 gccgatgtcg aggagcacca ccgtgggcgt cgcgcgctcg agttcgctgc gcatcgccgc
      181 gccgctcgcc gcctcgcgca cctcgtagcc atgcgcggac aggtactcga ccagcatcct
      241 ccgtaccgcc ggatcgtcgt cgaccaccag gatgcgcgtg acttccatgc gcgcagtata
      301 caaaacgaca acaaagtgtt tcgcgaacga aatcgcgcgg ataccccggc gccctacgct
      361 gcctcccatc gcaaccccac caaggaagca gcacatgaaa cggatcttca ggcttgccat
      421 cgatgctttc gtgagcgccc gcgaggagcg cgcccgcgcc cggctcgtct cgcagctcga
      481 cgagcgcacg ttgcgggaca tcggcctcga gcaggaggcg aaccacgcac gcgagcgcaa
      541 ccgcctcgcc atgcgattcg gagcgtactg acatgaaaag agacatcgat ctcgcgagcg
      601 agtacgtggt gcacggctcg ctcggcatga cgcgcggcag cgtgctgcgc atcgaggacg
      661 ggcgcgacgt ggcgctttac gtctgggaag gcgggctgtg gctaacacag gaaggggatc
      721 gccgcgaccg ctacctcggg ccgggcgact gcttcaggct cgagcgcgac ggcgtcacgc
      781 ttgcgcaggc catcgagccc agcacggtct cgctgaccgc gccgcagccc gagtcctacg
      841 ccgggcgcat cgcgcttacg aaagccggca ccggcgtgca ggttgagttg agcgccggcc
      901 ggaaaaggga cgcgctcggc ttctggacgc ggctcttcgc gcccgacgcg cggccgacga
      961 ccgcatcgct atgacgccgc ggcttcggcc acttacgcga caggacgcgg cgcgggtcca
     1021 ggcgttcgtg cgcgggttgt cttcgcagtc gcgctacgaa cgcttcctgg ctccgatcgc
     1081 cgagctatcc ccgcggcagc tcgagcgaat cactgccggg acggggctca gcctcggagc
     1141 tttcgacgca gccggcaggc tggtcggcct ggccgagtac gcccgctcgg gcaatgccga
     1201 ggcggagttc gccatcgtga tcgcggacga gttccagggg cagggactcg gcgccgagct
     1261 tgtcggggcg ctgctcgagc acgctaagcg ctccggcatc ctgcggctcg ccggcgtgac
     1321 ccacacgcgc aaccgtgcga tgcgcgtcct cgcgcgccgg ctgggctttg gcgtgcagcc
     1381 cgacgaggac ccggacctcg tgcgcatgga gctcgcgctc gccgcatgaa cgccgccgtg
     1441 ctctgcgctg gcgccaccgt gttcctctgg tacgcgttcg gtgcgctgcc gctgcatctg
     1501 tcggtcgtag aggagctcgg cctggatccc gcgcgcgcct cgagctggat ctcgatcgtg
     1561 tggatgagca gcgcagtgac gtcgatcgcc atatcgctgc gttatcggca gcccatcccg
     1621 atcacctgga ctatcccgag cctcgtgctg ctcggagctg tgggcggaaa cttcagcgcg
     1681 ccggagctcg cgggcgcctg cctcgccgcc ggggtcgcgc tcctcgtgct cgggatggcg
     1741 ggcgccggcg cggacgcgct cgagcgctcg ttcggcggcg agatgaagtt cggcgcgttg
     1801 gtcgcgcttg cggtggcggc tacgccgttc accgtgctcg gcgtcggtcc ggcgctctgg
     1861 gcgctgctgt gcggccttgc cgcttccgcg gccgcggagc gcacgcagct caccgctttc
     1921 tggaaggcag cttgaccatg ggaacgaacc tgtacgaacg cgcgatggca agcctgtggc
     1981 tgcagcgcgg cgacgcgcgc gctctggccg ccgccgcggt ggcggaggag ccggatgctg
     2041 ccgcaccacg actgctcgag gcggcgctgc tcgtgacgag ccggtacgtg aaggacttcg
     2101 aggccgcggg atgggcctac gcccggattc gcggcatgcc gatgaccgaa cgcgagcgcg
     2161 cgcatacctc ggcgctcgag gcggccgtgg acggcgacta tgagcgcgcc tgccttacct
     2221 atgaccgcat cctgctcggt acgccgaccg acgcgctcgc gctctggacc gcgcagctca
     2281 tggagtatta cctgggcaac tccgccgcga tgcgcgagcg ctccgggcga gtgctcgcgc
     2341 gctggtcggc cgacacgcct ggctaccacg ccgtcatgtc gatgcacgcc ttcgcgttgc
     2401 aggaatgcgg cgactacggc gccgcggaag aaatggcgct gcacgcgctc gagctcgagc
     2461 cgcgcgacct gcgcgcccag cactcgctga tgcatgtcta cgagatgctc ggcaagcccg
     2521 aggagggcat gcgctgggcc gcacgtcagg cgcagcagtg gtgcggcgat gccgccgtcg
     2581 cgcaccacct gtggtggcac accgcgctat tcctgacggc gctcgagcgc cggcacggcg
     2641 ccctggcggt ctacgacctg cgcctgcagc aggacaccat agccggaatg atcgacgcct
     2701 ccgcgctcct gtggcggctt catctcgagg gcgtcgacgt cggcgcgcgc ttcgccacgc
     2761 tcgccggccg ctgggcggcg cacgccgcgg acgcgtactg cgcgttcaac gacctgcatg
     2821 cgatgatggc cttcgccggc gccgcgcgct gggacctcgc gcagcagctg ctcgcggcgc
     2881 aggcgcgcag gatcgagc
//
LOCUS       DASUNT010000519         5516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_70011, whole genome shotgun sequence.
ACCESSION   DASUNT010000519 DASUNT010000000
VERSION     DASUNT010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70011"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VFX94_10465"
     CDS             complement(<1..757)
                     /locus_tag="VFX94_10465"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX5768647.1"
                     /translation="MPARSIPRRVAAGFFAAAAPDLDFIIGFIGPVEYLATHRGVTHS
                     LLLLPAWALLLSWILAKIVREPGGWRALYGVSALCLLVHIVGDLITSFGTMILAPLSD
                     WRAAIGTTFIIDLWFSGIIVAGLIASAVFYRSKIPAVAASVVLAGYVGFQYMQKQRAA
                     ELGERHATALGLRDAKVSVHPRPVSPFNWSVFVSDQESHRYAHFNLVRKEPRRYAPGD
                     GFIARIDSPYMPLEQAIWVTRPRYGASEQALIRE"
     gene            complement(830..2326)
                     /locus_tag="VFX94_10470"
     CDS             complement(830..2326)
                     /locus_tag="VFX94_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5768648.1"
                     /translation="MNCAERLVEIFGSQAEVARAFQLDRAVIHNWVKAGYVPARWAGE
                     VERVTQGRISALQIIDEASVKNPVRVKSRPENAPFGIYSEDNMTQYAPAKRIQSFHPP
                     QRTLMGPGPTEIHPRVLTTMSQPAIGYLDPIFVDMMEELKALLRYVYQTKNPLTFPLS
                     GPGSVGMEYCFVNMVAPGDKVIVCQNGVFGGRMLENAIRCGGNPVVVEDKWGEPVDPQ
                     KLEDALKKNKDAKLVAFVHAETSTGCQSDARLLTQIAHEHGALVIVDAVTSLGGSPVK
                     VDAWEIDAIYSASQKCLSCTPGLSPVSFSERVVEHVKKRTDKIHSWFMDMNLLLGYWN
                     ATTRTYHHTAPTNSLFALHEALLLIREEGLENSWARHLRHHVALKAGLEAMGLRFLVK
                     EQHQLPQMNAVVCPDAVNEEEVRRTLLGEFGLEIGAGLGPLKGKIWRFGLMGYSCRPD
                     NVMLCLSALGSVLADMGLPVHVGDAEAAAHGAYAQLHVKDAQRKKRAA"
     gene            2475..2810
                     /locus_tag="VFX94_10475"
     CDS             2475..2810
                     /locus_tag="VFX94_10475"
                     /inference="COORDINATES: protein motif:HMM:NF042861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HEX5768649.1"
                     /translation="MLAGLLDHWQVPLNDQAALLGLSAQSRSTIARYRRGEPLADSAD
                     LLARAGHLLGIHKALRIMFPHDRDLAYRWISTPNRRFAGARPLDIMKRQGYEGILAVR
                     RYLDFERGR"
     gene            2810..3496
                     /locus_tag="VFX94_10480"
     CDS             2810..3496
                     /locus_tag="VFX94_10480"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX5768650.1"
                     /translation="MPEGLVREVADYRGDLVRNIKGIRVSQDLFDDLARDAADREIAI
                     AAESATRIPSPAPLITRPFDYGTVITYPFAPHNWHATRYSNGLEYGVWYGALEIETTV
                     YETLYHWHRFVTDSFDLNREIVGERRVFRVHCEAILIDLRKSAEPRLIDRNDYAFTQQ
                     LGRYLWKRAQSGLLAPSARGPGTCAAIFRAEALSDVRDVCFLTYRLRANTATVERSPG
                     DTWLRITIPA"
     gene            3493..5226
                     /locus_tag="VFX94_10485"
     CDS             3493..5226
                     /locus_tag="VFX94_10485"
                     /inference="COORDINATES: protein motif:HMM:NF006203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX5768651.1"
                     /translation="MKSDHPVAFKPETAADAWLGLLAPRGIEYLFANGGTDFAPLVEG
                     YAKGRSLGWRLPQIVTVPHENMGIAMAHGYAMVTGRPQAMMVHVGVGTANSLNGLINA
                     SRQNVPILFTAGRTPLTESGAVPAARNNYIHWAQEHFDQGGMLREFVKWDYELRHAEQ
                     VETVLDRALAIAKSEPQGPVYVTLPREVLASPFPSSFSEAATIVPASPPAADPEALEE
                     AARLLGGAQHPLLVTASGGRSAASAHAIEQLARLLAVPVVHYRPRLLALSTEHPMHCG
                     WDPHPLLKEADVVLVVECDVPWIPKEGKPRTDAKVIHIGPDPLFASYPLRGFRADLAL
                     TGAVAPTLQALCRAAQKQAVSLQRIEERRKAVSLLSDEMRTKARAGVDAMPASITGKW
                     LSACVNKVLDDRTILVNEYPIVLEEMVIREPGRYFGNPSAGGLGWGMGAALGAKLASP
                     DKTVICAIGDGSYMFGNPTAAHYVSEAMRLPVLFVIANNARWAAVHRSTLSAYPKGFA
                     AAEQQPPFATLEPSPCFEHVVQASGGHGERVSRPHELIPALERALKIVKSEKRQAVLN
                     VCLEVSYVKTS"
     gene            5247..>5516
                     /locus_tag="VFX94_10490"
     CDS             5247..>5516
                     /locus_tag="VFX94_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768652.1"
                     /translation="MKFRALVAASVVLLAGFASQAGAQSRNLAPGFSALSLGSRVAVM
                     PTDIELFSVSAGGVLEPKADWTEAASRHFKNALIQKKTQALKLDVV"
BASE COUNT          958 a         1860 c         1761 g          937 t
ORIGIN      
        1 cctcgcggat cagcgcctgc tcgctcgctc cgtagcgcgg ccgggtaacc cagatcgcct
       61 gctcgagcgg catgtagggc gagtcgattc gcgcgatgaa gccatcgccc ggtgcatagc
      121 gccgcggctc cttgcgcacg aggttgaagt gcgcatagcg gtggctctcc tggtcgctca
      181 cgaagaccga ccagttgaac ggcgacaccg gacgagggtg cacgctcact ttcgcgtccc
      241 tcaagccaag cgctgtggcg tgccgctcgc caagttccgc cgcgcgctgc ttctgcatgt
      301 actggaagcc gacataaccc gcgagcacga cgctcgccgc cacggcggga atcttcgagc
      361 ggtagaacac cgccgaggcg atcaggccgg caacgatgat gccgctgaac cacaggtcga
      421 tgatgaaagt cgtgccgatc gctgctcgcc agtcggaaag gggcgcgagg atcatggtgc
      481 cgaagcttgt gattaggtcg cccacgatgt ggacaagcag gcacagcgcg ctcacgccat
      541 agagcgcacg ccagccgccg ggctcgcgca cgatcttcgc caggatccag gacaagagca
      601 gcgcccacgc cggtagtaga agcagcgaat gggttacgcc gcggtgcgtg gcgaggtact
      661 cgaccggtcc aatgaagccg atgatgaagt cgaggtcggg cgccgccgcg gcgaaaaacc
      721 cggccgccac gcgccgcggt atcgaccgcg ccggcacccc ggccggcgcg gtcgcgcgcg
      781 ccgccagcgc gcccgacagc gcgtgcgtca gcgtgtctat ttctctattc taggcggcgc
      841 gctttttccg ctgcgcatcc ttgacgtgca gctgcgcata cgcgccgtgc gcggccgcct
      901 cggcgtcgcc cacgtgaacc ggcagcccca tatccgcgag caccgagccg agcgccgaaa
      961 gacacagcat gacgttgtcc gggcggcagc tgtagcccat caggccgaag cgccagatct
     1021 ttcccttgag cggcccgagc ccggcaccga tctcgaggcc gaattcgccg agcagcgtac
     1081 ggcgaacttc ttcctcgttc accgcgtcgg ggcataccac cgcgttcatt tgcggtaact
     1141 gatgctgctc tttcacgagg aacctcaagc ccatcgcctc gaggccggcc ttgagcgcga
     1201 catgatggcg cagatggcgg gcccacgagt tctcgagtcc ctcttcgcgg atgagcagca
     1261 gcgcctcgtg cagcgcaaag agcgaattcg tcggcgccgt gtggtggtaa gtgcgcgtcg
     1321 tggcgttcca gtagccgagc agcaggttca tgtccatgaa ccagctgtgg atcttgtccg
     1381 tgcgcttctt cacgtgctcg acgacgcgct cggaaaacga gacgggcgac aggcccggcg
     1441 tgcacgagag gcatttctgg ctcgcggagt agatcgcgtc gatttcccac gcgtcgacct
     1501 tgaccgggga gccgccgagc gaggtcaccg catcgacgat cacgagcgcg ccgtgctcgt
     1561 gcgcgatctg ggtgaggagc ctcgcgtcgg actggcagcc cgtcgaggtc tcggcatgga
     1621 cgaaggcgac gagcttcgca tccttgttct tcttcaaagc gtcctcgagc ttctgcggat
     1681 cgaccggctc accccacttg tcttcgacca cgaccgggtt gccgccgcag cggatcgcgt
     1741 tttcgagcat gcgcccgccg aagacgccgt tctggcacac gatgaccttg tcgcccgggg
     1801 ccaccatgtt gacgaagcag tattccatcc ccaccgagcc gggcccggac agagggaagg
     1861 taagcgggtt tttcgtctgg tagacgtagc gcagcagcgc tttcagctcc tccatcatgt
     1921 cgacgaagat cgggtccagg tagccgatcg cgggctggct catcgtggtc agcacgcgcg
     1981 ggtggatctc cgtcggaccc gggcccatca acgtgcgctg cggcgggtgg aaggactgga
     2041 tgcgcttcgc gggggcgtac tgggtcatgt tgtcctcgga ataaatcccg aacggcgcgt
     2101 tctccggacg cgacttcacg cgtaccgggt tcttcacgct cgcttcgtcg atgatctgga
     2161 gcgcgctgat gcgtccctgg gtgacacgct ccacttcgcc cgcccagcgc gcgggcacgt
     2221 agcccgcctt tacccagttg tggatcaccg cgcggtccag ctgaaacgcg cgtgcgactt
     2281 cggcctggct gccgaaaatc tcgaccagtc gctcagcgca attcattgac ccaagcttat
     2341 tgtgtcaacc ttgtcttgac aatacgccga cagtctgtac ttattggtac gtagttggta
     2401 cgctttgaga catgcgcgaa ccagcgccgc aagtcgacct tcattcgcgg gcgagccggg
     2461 agcggctggc aaggatgctg gccggactgc tggaccattg gcaagtgccg cttaatgacc
     2521 aggcggcgct gctcgggctg tcggcccagt cgcgctcgac cattgcgcgc taccggcgcg
     2581 gcgagccgct cgccgacagc gccgatctgc tcgcccgcgc aggacatctg ctcggcatcc
     2641 acaaggcgct gcgcatcatg ttcccgcatg atcgcgatct cgcctaccgc tggatcagca
     2701 cgccgaaccg gcgatttgcg ggcgcgcggc cgctcgacat catgaaacgc cagggctacg
     2761 aggggatcct cgcggtaagg cgctacctgg acttcgagcg cggccggtag tgcccgaggg
     2821 tctggtccgc gaggtcgccg actaccgcgg cgacctggtg cgcaacatca agggcatccg
     2881 ggtctcccag gatcttttcg acgacttggc gcgcgacgcc gccgacaggg agatcgcgat
     2941 tgcggcggaa agcgcgaccc gaatcccgag cccggcgccg ctgattaccc gccctttcga
     3001 ctacggcacg gtaatcacgt atcccttcgc gccgcacaac tggcatgcca cgcgctactc
     3061 caacggcctc gagtacggcg tgtggtacgg cgcgctcgaa atcgaaacca cggtatacga
     3121 aacgctctac cactggcacc ggttcgtaac agacagtttc gatctgaacc gggaaatcgt
     3181 gggggagcgg cgcgtgttcc gggtgcattg cgaagccatc ctgatcgacc tgcgcaaaag
     3241 cgccgagcca cgcctgatcg accgcaacga ctacgccttc acgcagcagc tcgggcggta
     3301 tctctggaag cgcgcgcaga gcgggctgct tgccccctca gcccgcggcc cgggcacttg
     3361 tgcggcgatt ttccgcgccg aagcgctgtc ggacgtgcgc gatgtctgct tcctcaccta
     3421 ccgcctgcga gccaacaccg ccaccgtgga gcgctcgccc ggggatactt ggctcaggat
     3481 tactattccc gcatgaaatc cgaccacccc gtcgccttca agcccgaaac cgcagccgac
     3541 gcctggctgg gactgctggc gccgcgcggc atcgaatacc ttttcgcgaa cggcgggacg
     3601 gacttcgcgc cgctcgtcga gggctacgcc aagggacgca gcctcggctg gcgcctgccg
     3661 cagatcgtca ccgtcccgca cgagaacatg ggcatcgcca tggcgcacgg ctatgccatg
     3721 gtcaccggcc ggccgcaggc gatgatggtg cacgtcggcg tcggcaccgc gaactcgctg
     3781 aacggactga tcaacgcctc gcgccagaac gtgccgatcc tcttcaccgc cggacgcacg
     3841 ccgctcaccg agtcgggcgc tgtgccggcg gcgcgcaaca actatatcca ctgggcgcag
     3901 gagcacttcg accagggcgg catgctgcgc gagttcgtga aatgggacta cgagctgcgc
     3961 cacgccgagc aggtcgagac ggtgctcgac cgcgcgctcg cgatcgcgaa aagcgagccg
     4021 caaggacctg tctacgtcac cttgcctcgg gaagtcctcg cttcaccgtt cccgagctcg
     4081 ttctccgaag cagcgaccat cgtcccggcg tctcccccgg ccgccgaccc agaggcgctc
     4141 gaagaagcgg ccaggcttct cggcggcgcg cagcatcccc tgctcgtcac ggcgagcggc
     4201 gggcgcagcg ccgcttccgc gcacgcgatc gagcagctcg cccggctgct cgcggtacct
     4261 gtcgtgcact accgcccgcg gctcctcgcg ctctccaccg agcacccgat gcattgcggc
     4321 tgggatccgc atccgctgct gaaggaagcc gacgtcgtgc tagtggtgga gtgcgacgtg
     4381 ccctggatcc cgaaggaagg aaagcccagg acggacgcca aggtcatcca tattggaccc
     4441 gatccgctct tcgcctccta cccgctgcgc ggctttcgcg ccgacctggc gctcaccggc
     4501 gcagtggcgc ccacactgca agcgctctgt cgcgctgccc aaaagcaagc cgtgtcgctc
     4561 cagcgaatcg aggagcgtcg caaggccgtt tcgctcctct ccgacgagat gaggacaaag
     4621 gcgagagcgg gggtcgatgc catgccggcg agcatcacgg ggaaatggct ctccgcctgc
     4681 gtgaacaagg tgctcgacga cagaaccatt ctcgtcaacg agtatcccat cgtgctcgag
     4741 gagatggtga tccgcgagcc gggccggtat ttcggcaacc cctcggcggg cggcctgggc
     4801 tggggcatgg gtgccgcgct cggcgcgaag ctggcctcac cggacaagac cgtgatctgc
     4861 gcgatcggcg acgggtctta catgttcggc aaccctaccg ctgcgcatta cgtgtccgag
     4921 gcaatgcggc tgccggtgct cttcgtgatc gccaacaacg cacgctgggc cgcggtgcac
     4981 cgatctacgc tctccgctta cccaaaaggc ttcgctgcgg ctgagcagca accgcctttc
     5041 gcgacccttg agccatcgcc gtgcttcgag cacgtggtgc aggcgagcgg cggccatggc
     5101 gagcgcgtaa gccggccgca cgagctcatt ccagccctcg agcgcgcgtt aaagattgta
     5161 aagagcgaaa agcgccaggc agttctgaat gtctgcctgg aggtaagcta tgtgaagacc
     5221 agctagggga gaagcaaagg gggtccatga agtttcgtgc actggtcgcg gcgtcggtcg
     5281 tgctgctcgc cggtttcgcc tctcaggcgg gcgctcaatc aagaaatctt gcgccggggt
     5341 tctccgcgct ctcgctcgga tccagggtgg ccgtcatgcc caccgacatc gagctctttt
     5401 ctgtcagcgc gggcggcgtg ctcgagccca aggccgactg gactgaagcc gcgtcgcgcc
     5461 acttcaagaa tgctctcatt cagaagaaaa cccaggctct gaagctcgac gtcgtc
//
LOCUS       DASUNT010000520         5663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1121910, whole genome shotgun sequence.
ACCESSION   DASUNT010000520 DASUNT010000000
VERSION     DASUNT010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121910"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFX94_10495"
     CDS             complement(<1..334)
                     /locus_tag="VFX94_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX5768653.1"
                     /translation="MNPARPANPRTERLLAAPIVPALARLSAPGLLLVAFQSMVSVGD
                     TYFVGRLGTAPLAGLALVFPLLMLLQMTSAGAMGGGVSSAIARALGSGDAATARRLVV
                     HALVIAAAM"
     gene            387..1145
                     /locus_tag="VFX94_10500"
     CDS             387..1145
                     /locus_tag="VFX94_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX5768654.1"
                     /translation="MELLQDPQVWIAFVTLTALELVLGIDNIIFITILVDKLPPAQRE
                     RARRIGLGAAMVMRIGLLLVLAWIIGLTEPLFTFFNERISGRDLILISGGLFLLWKSV
                     VEIHQLLEGEEGERSAAVGASFSAVIGQIMVIDLVFSLDSIITAVGMVPWIEVMIAAV
                     IASVGLMMAFAGPIGRFVSAHPTIKMLALSFLVLIGVLLIAGGFDHDVPKGYVYFALA
                     FSVGVEMLNIRLRKRSKPVHLHEPYSRGEPPARD"
     gene            complement(1112..1492)
                     /locus_tag="VFX94_10505"
     CDS             complement(1112..1492)
                     /locus_tag="VFX94_10505"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX5768655.1"
                     /translation="MRRMLVAAGWAWASAIVWLSLTPSPPELELAHGDKLGHLAAYGL
                     LMFWFAQLHAARASRVAHASAFVAMGVALEFAQGALGYRNYDIADMYANTLGVGLGWV
                     LGAMLPPLLPRAGAQSRAGGSPRL"
     gene            complement(1492..2568)
                     /locus_tag="VFX94_10510"
     CDS             complement(1492..2568)
                     /locus_tag="VFX94_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX5768656.1"
                     /translation="MKDDPRYLRGARSFVQGRFETVAREAFDDGWTPEEADTRELKTS
                     VAIERARSIVSHNDSPDVGFSQSINPYRGCEHGCVYCYARPSHAYLELSPGLDFETKL
                     FAKTNAAELLRAELARPGYKPSPIALGANTDCYQPIERKYRITRQILEVLAGCEHPVT
                     MVTKSALIERDLDILGPMGRKNLVKVLVSIGTLDRELARRLEPRAASPQRRLDVVRKL
                     NESGVPCGVMIAALIPALNDKTMENVVEAAAAAGAKEAAYVILRLPYELKEVFKEWLA
                     EHYPQRAQHVMSIVRQMRGGRDNDPCFGTRMSGTGNYAELIEKRFELACRRFGLNRRE
                     APDLDCSRFSPPAPSAAGGQLQLL"
     gene            2567..3769
                     /locus_tag="VFX94_10515"
     CDS             2567..3769
                     /locus_tag="VFX94_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5768657.1"
                     /translation="MPDTVYTNSTRRNVALLAACQALLFTNNSTLIAINGLAGLALAP
                     YVGLATLPVTGWVLGGAIGTMPASLHMRRVGRQRGLTSGTLWGVVGALICAAAIWLQS
                     FWLLCFGTLVFGVYNAYGQYYRFAAADVATGDFKAVAISLVLAGGLVGGILGPTTSRF
                     TIELLAPKFMGAYFVLIAFALATMILLRFVRIPTQGAAERAGPGRSLREIAAQPKYLV
                     AVAAGAIGYGVMNFLMTSTPIAMQVCGHPYGDAAFVISSHVIGMFAPSFVTGPMIKRV
                     GALPVMLAGALLNFAAIGIALAGIAVANFWWSLVILGVGWNCLYIGGTTLLTETYRPE
                     ERARAQGMNEQAIFTMMAISSLTSGLTVTTAGWERVNVLALPLVALVAVAIVWFALRE
                     RARSAASA"
     gene            3766..4860
                     /locus_tag="VFX94_10520"
     CDS             3766..4860
                     /locus_tag="VFX94_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEX5768658.1"
                     /translation="MSQPAPAAVGARLEDVDTPALILDLDAFERNQRTLFDLARGRVR
                     VRPHAKTHKCPEVARRQVAMGAVGMCCQKVSEAEAMVDGGIADVLVSNEIVGGRKLER
                     LAGLSRRAKIGVCVDNAENLQAVAATGAKLDVYIEIDVGMRRCGVPPGRPAVELAKAI
                     SETKHLRFAGLQAYHGRAQHIRSMEERHRAIELAAQHVEHTKRLLAEAGIPCPRVTGA
                     GSGTFMLEVEAGVWDEIQPGSYAFMDADYAKNEWAAPLPRFEHALFVLTTVMSRPSPA
                     MAIVDAGLKASSVDSGMPVVWQRPGLNYTRASDEHGWVEGDPTPALGERLLLVPGHCD
                     PTINLYDWYVVFKEGVVVDLWPITARGAVL"
BASE COUNT          909 a         1874 c         1888 g          992 t
ORIGIN      
        1 ccatcgcggc cgcgatgacg agcgcatgca ccacgaggcg gcgcgccgtg gcggcatcgc
       61 cggagccgag cgcgcgcgca atggcggagg aaaccccgcc tcccatcgcc ccggcggagg
      121 tcatctgcag cagcatgaga agcgggaaga cgagcgcgag gccggcgagc ggcgcggtgc
      181 cgaggcggcc gacgaaatag gtgtcgccga ccgagaccat cgactggaag gcgacgagca
      241 gtagtccggg cgccgacagt cgggcgagcg ccggcacgat cggcgccgcg agcagccgct
      301 cggtgcgcgg gttcgcgggg cgcgcaggat tcatggggcg cgccattgta tgatcgcgcg
      361 ccggcccgca agaggaggac gccccgatgg agctgcttca ggacccccag gtctggatcg
      421 ctttcgtcac gctgaccgcg ctcgagctcg tactcgggat cgacaacatc atcttcatca
      481 cgatcctggt ggacaagctg ccgcccgcgc agcgcgagcg cgcccgccgc atcggcctcg
      541 gcgccgcgat ggtaatgcgc atcgggctgc tactcgtgct cgcttggatc atcggcctca
      601 cggagccgct cttcacgttc ttcaacgaaa ggatctccgg ccgcgacctc atcctcatca
      661 gcgggggcct cttcctgctc tggaagagcg tggtggagat ccaccagctc ctcgagggcg
      721 aagagggcga gcgctccgcc gccgtgggtg cgagcttctc cgcggtgatc ggccagatca
      781 tggtgatcga cctcgtcttc tccctcgact cgatcatcac cgcggtcgga atggtgccct
      841 ggatcgaggt gatgatcgcg gcggtgatcg cgtcggtcgg gctcatgatg gcgttcgccg
      901 ggccgatcgg gcgcttcgtt tcggcgcacc cgacgatcaa gatgctcgcg ctctcgttcc
      961 tggtgctgat cggcgtgctg ctgatcgcgg gcggcttcga ccatgacgtg ccgaagggct
     1021 acgtctattt cgcgctcgct ttctccgtcg gcgtggagat gctcaacatc cggctgcgca
     1081 agcgctcgaa gcccgtgcat ctgcacgagc cctacagccg cggcgagccc cctgcccggg
     1141 actgagctcc ggcgcgcggc agcagcggcg gcagcatcgc gccgagcacc cagccgaggc
     1201 cgacacccag cgtattggcg tacatgtccg cgatgtcgta gttgcggtag ccgagcgcgc
     1261 cctgcgcgaa ttccagggcg acccccatcg cgacgaacgc gcttgcgtgc gcgacgcggc
     1321 tcgcgcgcgc ggcatgaagc tgcgcgaacc agaacatgag cagcccatag gcggccaggt
     1381 gcccgagctt gtcgccgtgg gcgagctcga gctccggcgg cgacggcgtg agcgacaacc
     1441 atacgatcgc actcgcccac gcccagcccg cggcgaccag catccttctc attacagcaa
     1501 ctgtagctga cctccggccg cggacggcgc cggcgggcta aatcggctgc aatcgagatc
     1561 cggcgcctcc ctgcggttca atccgaagcg gcggcacgcc agttcgaagc gcttctcgat
     1621 cagttcggcg taatttcccg taccggacat ccgcgtgccg aagcaaggat cgttgtcgcg
     1681 gccgccgcgc atctggcgca cgatgctcat gacgtgctgc gcgcgttgcg gatagtgctc
     1741 ggcaagccac tccttgaaca cctccttcag ctcgtagggc aggcgcagga tgacgtaggc
     1801 ggcctctttg gcgcccgctg cggcggccgc ctcgaccacg ttctccatcg tcttgtcgtt
     1861 cagcgccggg ataagcgcgg cgatcattac cccgcacggc accccgctct cattcagctt
     1921 tctcaccacg tcgaggcgcc gctgagggct cgcggcgcgc ggctcgagcc tgcgcgcgag
     1981 ctcccggtcg agggtgccga tcgagacgag caccttcacc aggttcttcc tccccatcgg
     2041 cccgagaata tcgagatccc tttcgatcag cgccgacttg gtgaccatcg tcaccgggtg
     2101 ctcgcatccg gccagcactt ccaggatttg ccgggtaatg cggtacttgc gctcgatcgg
     2161 ctggtagcag tcggtgttcg cgccgagcgc aatcgggctc ggcttgtagc cgggcctggc
     2221 gagctcggcg cgcagcagtt ccgccgcgtt cgtcttggcg aagagcttcg tttcgaagtc
     2281 gaggccgggc gaaagctcga gatacgcatg gctcggcctc gcgtagcagt acacgcagcc
     2341 atgctcgcat ccccgatagg gatttatcga ctgcgagaag ccgacgtcgg gcgaatcgtt
     2401 gtgcgagacg atggagcgcg cgcgttcgat ggcgacgctc gtcttcagct cgcgcgtatc
     2461 ggcttcctcc ggcgtccagc cgtcgtcgaa agcctcgcga gccacggttt cgaaacgacc
     2521 ctgcacgaag gagcgcgctc cacgaagata gcgggggtcg tccttcatgc ccgatactgt
     2581 atatacgaac agtaccaggc gcaatgtcgc gcttctggcg gcgtgccagg cgctgctatt
     2641 cacgaacaat tcgacgctga tcgccatcaa cggcctggcc ggcctcgccc tcgctcctta
     2701 tgtgggactg gccacgctac cggtgaccgg gtgggtcctc ggtggcgcga tcggcaccat
     2761 gccggcctcc ctccacatga ggcgcgtcgg gcgtcagcga gggctcacca gcggcaccct
     2821 gtggggcgtc gtgggcgcgc tcatctgcgc ggcggcgatc tggctgcaga gtttctggct
     2881 gctgtgcttc ggcacgctcg tgttcggcgt ttacaacgcg tacgggcagt actaccgctt
     2941 cgccgcggcc gacgtggcga caggcgattt caaggcggtc gcaatttcgc tcgtgctggc
     3001 aggcggcctg gtcggcggca tcctgggtcc gaccacgagc cgcttcacga tcgagctgct
     3061 cgcccccaaa ttcatgggcg cgtatttcgt gctgatcgcc tttgcgctgg cgacgatgat
     3121 cctgctgcgg ttcgtccgca tcccaacaca gggcgctgcc gagcgcgcgg gacccggccg
     3181 ctcgctgcgc gagatcgcgg cgcagccgaa gtacctcgtg gcagtcgccg ccggcgcgat
     3241 cggctacggg gtcatgaatt ttctgatgac ttccacgccg atcgcgatgc aggtctgcgg
     3301 ccacccttac ggcgacgcgg cgttcgtcat ctcgtcgcac gtcatcggca tgttcgcgcc
     3361 gtccttcgtc accgggccga tgatcaagcg cgtcggcgcg ctgccggtca tgttggcggg
     3421 cgcgctgcta aacttcgcgg ccatcggcat tgctctagcc ggtatcgcgg tggcgaactt
     3481 ctggtggtcg ctcgtgatcc tcggcgtggg gtggaactgc ctctatatcg gcggcacgac
     3541 tctgctcacc gaaacctacc gcccggagga gcgggcgagg gcgcagggca tgaacgaaca
     3601 ggcgatcttc accatgatgg cgatctcttc gctcacctcc ggccttaccg tgacgacggc
     3661 cggctgggag cgcgtcaacg tcctcgcgct ccccctcgtc gccctggtcg ccgtggcgat
     3721 cgtgtggttc gcgctgcgcg aacgcgcgcg cagcgcggcg tcggcatgag ccagcccgca
     3781 ccggcggccg tgggcgcacg gctggaagac gtcgacacgc cggcgctgat cctcgatctc
     3841 gatgctttcg agcgaaacca gcgcacgctg ttcgacctcg cgagaggtcg cgtgcgagtg
     3901 cggccgcatg ccaagaccca caagtgcccg gaggtcgccc ggcggcaggt cgcgatgggc
     3961 gcagtcggca tgtgctgcca gaaggtgtcg gaagccgagg cgatggtcga cggcggcatc
     4021 gccgacgtgc tggtctcgaa cgagatcgtg ggcgggcgca agctcgagcg tctcgcgggg
     4081 ctttcgcgcc gggcgaagat cggggtttgc gtggacaacg ccgagaacct gcaagccgta
     4141 gccgcaaccg gcgcgaagct tgacgtctac atcgagatcg atgtcggcat gcgtcgctgc
     4201 ggtgtgccgc ccggacgacc cgccgtcgag ctcgcgaagg cgatttcaga gacaaagcat
     4261 ctgcgcttcg cgggattgca ggcctatcac ggccgcgcgc agcacatccg cagcatggaa
     4321 gagcgccacc gcgccatcga gctcgcggcg cagcacgtgg agcacacgaa aaggcttctc
     4381 gccgaagcgg gcattccgtg tccgcgggtc accggcgcgg gcagcggcac cttcatgctc
     4441 gaggtcgaag cgggcgtctg ggacgagatc cagcccgggt cctatgcctt catggatgcc
     4501 gactacgcga aaaacgagtg ggccgcgccg ttgccgcgct tcgagcacgc gcttttcgtg
     4561 ctcacgaccg taatgagccg cccttcgccc gccatggcca tcgtcgatgc ggggctcaag
     4621 gcctccagcg tcgactcggg catgccggtg gtgtggcaac ggccggggct gaactacacc
     4681 cgcgcctcgg acgagcacgg ctgggtcgaa ggcgacccga cccccgcgct cggcgagagg
     4741 ctgcttctcg tgccggggca ttgcgacccg accatcaacc tctacgactg gtacgttgtc
     4801 ttcaaagaag gtgtcgtagt cgacctctgg ccaatcaccg cccgaggggc cgtgctttag
     4861 ctcccacact acagggtcca gcgccgttct tgaatcgatg acggaagagc cacagcagca
     4921 gcgcgatgac ggagccgccc ggaagcagca gctcaatggc gagatacggg ctggcattgc
     4981 gtgcgacctg aattagtgcg ctcatgatgc cgttaccctc tgccattcag gcgcgccgga
     5041 gatcggtgca tcctggtgag aggaatgcta cgaggaaagc gcgacataga cagcgaacaa
     5101 cagcgctggc caagtgtgac cgattggccc gcgggaggcg catctccggc tgtctatcga
     5161 tcttgttgaa cagcggaaat gcttgttcgg gggccctggc atcgattgcc gtgctgactc
     5221 aacgtcctgt ctgcgggtcc catacgccgg ccgacccgag ccggctggga ttgtgagccg
     5281 cagcgtcgat tggtccaatt cgtccgtggc ttcaaccgct acggattcga gcttcaccga
     5341 cttgatgagt tggccaagtt ggtcgccgag atggatctgt tagccgagcc ggccaaattg
     5401 ctcgacgaga tggaggcact cttggaccgg gcaaaatccc tagctccagt gacgcgcgaa
     5461 tgagacgagg cgcacagcga acgcggtctc gctagttcga aggcctcgcg gccttcggca
     5521 gttcctgttt cggctgagca gacccaaaaa gcccgcattc gcgggctttt caggcagagc
     5581 aaggctgcgc tactgtttct ttggcggctc gctttggccc gtggcgcctg cgccggcgcc
     5641 ctgaggcgtt atctcgtctc ggg
//
LOCUS       DASUNT010000521         2367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1033969, whole genome shotgun sequence.
ACCESSION   DASUNT010000521 DASUNT010000000
VERSION     DASUNT010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033969"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFX94_10525"
     CDS             complement(<1..264)
                     /locus_tag="VFX94_10525"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEX5768659.1"
                     /translation="MALLLPLSVAAAEVAGVKLEDKAQVGNAEIALNGAGLRKRLVFQ
                     VYVVGLYLPQKSSTAEAVLAQPGPKRIAIHMLRDVSADDFTDAL"
     gene            complement(275..1147)
                     /locus_tag="VFX94_10530"
     CDS             complement(275..1147)
                     /locus_tag="VFX94_10530"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX5768660.1"
                     /translation="MKVDLAHVEEVAKELYIRALKALPPDIKRGFDTLAAAESDAGAK
                     RMLGTMIRNIAVAEATDNLLCQDTGIPVYNVWIGRGVEVDGVGLKEAIRRGCARSTRE
                     YAFRSSIVHPTTRVNEQNSTGRGVPVINIDFSPEESLVIEMIPKGSGSENNSWLRMAI
                     PAEGVDAIKTFVVDCVLEAGGKTCPPTVVGVGVGGTADLCVHLAKVAATRALGSRCED
                     PEGAKLEAALTEAVNMLGVGPQGLGGDSTAFAVHVETAATHITMNPVAVNMQCHSARR
                     ARATISSSGVTYGF"
     gene            complement(1144..2100)
                     /locus_tag="VFX94_10535"
     CDS             complement(1144..2100)
                     /locus_tag="VFX94_10535"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5768661.1"
                     /translation="MKKAVACLLAALFALSAQAQSWPQKPVRFIVPFPPGGATDISAR
                     LLGEKLTQLWGQTVVIENRGGAGGAVGAAEAARAAPDGYTLFFPSGSVVTANQHVYAK
                     LAYDPEKDFIPVTKVVSGPQVLTVPANSPFKTVKDLIDHARANPGKLTFGHAGIGSQT
                     HLAAENFVWQAKIDAVAVPYKGEGPGLAAAVAGETSFFLGNVAAAIGHITSGRLRALG
                     VTSKDEAAQLRGVPPIGKTIPGFENTGWFGIVAPAGTPKQIVQKVYLDTRKALEASDL
                     RGRLFVQGLAPVGNTPEQMAKEMKEESALWARVVKERNIQVK"
     gene            complement(2122..>2367)
                     /locus_tag="VFX94_10540"
     CDS             complement(2122..>2367)
                     /locus_tag="VFX94_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEX5768662.1"
                     /translation="LFLPLHFWTLGNALQGSLAWTDQPLVKLGEWLIVLALAAHLGGG
                     LRVLALEFLPWRDWQKSLTAAAAALALAVGLALALAL"
BASE COUNT          378 a          827 c          761 g          401 t
ORIGIN      
        1 caatgcatcc gtgaagtcgt cggcgctcac gtcgcgcagc atgtgaatgg cgatgcgctt
       61 gggtccgggc tgggcgagca ccgcctccgc agtggagctt ttctgcggaa gataaaggcc
      121 gaccacgtag acctggaaga ccaggcgttt gcgcaagccg gcgccgttca acgcgatctc
      181 ggcgttcccc acctgcgcct tgtcttcgag ctttaccccc gccacttccg ccgcggcaac
      241 ggataacggc aggagcaacg ccaccagaag aaacctaaaa gccatatgtc actccgctgg
      301 agctgatggt cgcgcgcgcc cggcgcgccg agtggcattg catgttgacc gcgaccggat
      361 tcatggtgat gtgcgtcgcc gctgtctcga cgtgcacggc gaacgcggtc gagtcgccgc
      421 caagaccctg cgggccgacc ccgagcatgt tgaccgcctc ggtgagcgcg gcctcgagct
      481 tcgcgccctc gggatcctcg cagcgcgagc cgagcgcccg cgtcgccgcc accttcgcca
      541 ggtgaacgca caggtccgcc gtgccgccaa cacccacgcc gacgacggtg ggcgggcacg
      601 tttttccgcc ggcttcgagc acgcagtcga cgacgaaggt cttgatcgcg tcgacgccct
      661 cggcgggaat ggccatgcga agccacgagt tgttctccga ccccgagcct ttcgggatca
      721 tctctatgac gagagattct tcaggcgaaa aatcgatgtt gatcaccggc acaccgcgcc
      781 cggtggagtt ctgctcgttc acgcgcgtgg tcgggtgcac gatcgaggag cggaaagcgt
      841 actcgcgcgt cgagcgcgcg cagccgcggc ggatcgcttc cttcagcccc acaccgtcca
      901 cctccacgcc gcggccgatc cacacgttgt acaccgggat cccggtgtcc tggcacaaca
      961 ggttgtcggt ggcctcggcg acggcgatgt tgcggatcat ggtgccgagc atgcgcttcg
     1021 cgccggcgtc gctctcggcc gcggcgagcg tgtcgaagcc gcgcttgatg tccgggggca
     1081 gcgccttcag cgcccggatg taaagctcct tggcgacttc ctctacgtgt gcaaggtcaa
     1141 ccttcacttg acctggatgt tgcgctcctt caccaccctc gcccagagcg cgctctcttc
     1201 cttcatctcc ttcgccatct gctccggcgt gttgccgacc ggggcgaggc cctggacgaa
     1261 aagtcgccca cgcaggtcgc tcgcctcgag cgccttgcgc gtgtcgaggt agaccttctg
     1321 cacgatttgc ttcggtgttc cggccggcgc cacgatgccg aaccatcccg tgttctcgaa
     1381 ccccgggatc gtctttccta tcggcggcac gccgcgaagc tgcgccgctt cgtccttgct
     1441 cgtcacgcca agcgcgcgca gccggccgct ggtgatgtgg ccgatcgccg ccgccacgtt
     1501 gccgaggaag aagctcgtct cgcccgccac ggccgccgcc aggcccgggc cctcgccctt
     1561 gtagggcacc gcgaccgcat cgattttcgc ctgccacacg aagttctcgg ccgcgaggtg
     1621 ggtctgcgag ccgatgccgg cgtggccgaa cgtcagcttg ccgggattgg cgcgcgcgtg
     1681 gtcgatcagg tccttcacgg tcttgaacgg agagttggcc ggcacggtga gcacctgcgg
     1741 ccccgagacc actttggtga ccggaatgaa atccttctcc gggtcgtacg cgagcttcgc
     1801 gtagacatgc tggttcgccg tgacaaccga tccggacgga aagaagagcg tgtacccgtc
     1861 aggcgccgcg cgcgccgctt cggcagcgcc cacggcgccg ccagcaccgc cccggttctc
     1921 gatcaccacc gtctggcccc agagctgcgt cagcttctcg ccgagcagtc gcgccgagat
     1981 gtcggtagcg ccgcccggcg gaaacggcac gatgaagcgc acgggtttct gcggccagga
     2041 ctgcgcctgc gcgcttagcg caaagagcgc agccaaaaga catgcgacgg cctttttcat
     2101 ttgatcctcc tcgcgaggat tctacagcgc cagcgcgagc gcgagcccga ccgcgagcgc
     2161 aagcgcagcc gcggcggccg tcaggctctt ttgccagtcg cgccagggca ggaactccag
     2221 cgcgagcacg cgcagcccgc cgccgaggtg cgccgcgagc gcaagcacga tgagccattc
     2281 gccgagcttg acgagcggct ggtcggtcca ggcaagcgag ccttgcagcg cgttgccgag
     2341 tgtccagaag tgcagcggca ggaacag
//
LOCUS       DASUNT010000522         2391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1034108, whole genome shotgun sequence.
ACCESSION   DASUNT010000522 DASUNT010000000
VERSION     DASUNT010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034108"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VFX94_10545"
     CDS             <1..393
                     /locus_tag="VFX94_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5768663.1"
                     /translation="IDVDNLEKGTRFYCDGLGLRVGRRFDGWVELLGADASIYLLPKP
                     AGTEAFPKGSTRSYVRHWTPVHLDFVVPHIGEAVKRAVAAGATLEREVTEHAYGKLAL
                     LADPFGNGFCLLQFTDRGYDAIALSGRQ"
     gene            complement(379..933)
                     /locus_tag="VFX94_10550"
     CDS             complement(379..933)
                     /locus_tag="VFX94_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5768664.1"
                     /translation="MPPLDPATMTPAQAKAAQALTDGPRGGVRGPFIPLLRSPELMDR
                     LQKLGEYLRFHSALPARISELVILAVARQWTQQFEWSVHVALAREAGVKPQTLASLSE
                     GRRPDAMAIDEEIAYDFCAELVHNKGVADATYRRAVQQFGEQGVIDMLGLIGYFTTVS
                     MVMNVACTPAPKNDGASMLPSLPT"
     gene            complement(944..2116)
                     /locus_tag="VFX94_10555"
     CDS             complement(944..2116)
                     /locus_tag="VFX94_10555"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEX5768665.1"
                     /translation="MRPALDSWQPRESAASVAAAGLIALAVAMGIGRFAFTPILPMMQ
                     DDAGLSIARGSWLASANYVGYFLGALWATVHRVRSPLAIRAALLVIGLATLAMGLVEQ
                     FAAWLVLRMLAGVASAWALIHVSAWCLERLTSLRRPLLIGVVFTGVGSGIALAGGMCL
                     ALMSIRASSWLAWASLGMVSLVVAALIWPVVDEGAAPTKRGRTGAGRRQWTPRMLQLV
                     FCYGAFGFGYIIPATFVPVMAKQAIADPALFGWAWPVFGLTAAASTLLSAVWSRAGAY
                     RRVWIVASAAMAAGVAAPVALPGLPGVLMAAVGVGGTFMVITMAGMQEAGRVAGAAAP
                     ALIGAMTAAFALGQIIGPLTAGLASDLWISHLIASALLVLSAVVLAIEPDERSSRG"
     gene            complement(2082..>2391)
                     /locus_tag="VFX94_10560"
     CDS             complement(2082..>2391)
                     /locus_tag="VFX94_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX5768666.1"
                     /translation="RSIVFDISRRNVWPAPFTGRCMRNPYVERWYGREVELMQRVDQE
                     AARLAEARRKHDFDIAPVIAGEACGLVRDILPAREIVDRIMREAEGLLASGARQLAAA
                     "
BASE COUNT          451 a          817 c          774 g          349 t
ORIGIN      
        1 atcgacgtgg acaacctcga gaagggcacc cgcttctatt gcgacgggct cgggctccgc
       61 gtgggcaggc gcttcgacgg ctgggtggag ctcctgggcg ccgacgcgtc gatttatctg
      121 cttccgaaac cggctggaac agaggccttc ccgaaaggct cgacgcggag ctacgtccgc
      181 cactggacgc cggtacatct ggacttcgtc gtcccccaca tcggcgaggc tgtgaagcgc
      241 gcggtggccg ccggggcgac gctcgaacgc gaggtaacgg agcacgcgta cggcaagctc
      301 gcgcttcttg ccgatccttt cggaaacggc ttctgcctgc tgcaattcac cgaccgcggc
      361 tacgacgcga tcgcgctctc aggtcggcaa tgagggcagc atgcttgcgc cgtcgttttt
      421 gggcgcaggc gtgcaagcaa cattcatcac catcgaaacc gtggtgaagt agccgataag
      481 cccgagcata tcgatcacgc cctgctcgcc gaactgctgc accgcccgcc gataagtcgc
      541 gtcggctacg cccttgttat gcacaagctc ggcgcagaag tcataggcga tctcttcgtc
      601 aatcgccatg gcgtcgggcc tgcgcccttc gcttaggctc gccagcgttt gcggctttac
      661 ccctgcctcg cgtgcgagcg ccacgtgtac ggaccattcg aactgctgtg tccattggcg
      721 tgcgaccgcc agaatcacga gctcgctgat ccgcgccggc agcgcgctgt gaaaacgcag
      781 gtattcgccg agcttttgca gccggtccat gagctcggga ctgcgcagca ggggaatgaa
      841 cggccctctc acgccgccgc gcgggccgtc ggtaagcgcc tgcgcagcct tggcttgcgc
      901 aggcgtcatg gttgcgggat cgagcggcgg catccggtcc gggctagcca cggctactcc
      961 tttcatcagg ttctattgcg agcactaccg cgctaagcac tagcagcgcg ctagcgatga
     1021 gatgcgaaat ccaaaggtca ctagcgaggc cggcggtcag cgggccgatg atctggccca
     1081 aggcaaacgc cgccgtcatg gcgccgatga gcgccggagc agccgcgccc gccacgcgtc
     1141 ctgcctcctg catgccggcc atggtgatga ccatgaacgt tcccccgacg ccaacggctg
     1201 ccatcagcac gcccggcaga ccgggaagcg cgaccggcgc agccaccccg gcagccatcg
     1261 ccgccgaggc gacaatccag acgcggcggt aggcgccggc ccgactccac acggcgctca
     1321 gcagcgtgga ggcggccgcg gtcaagccga acaccggcca ggcccagccg aagagcgcgg
     1381 ggtcggcgat cgcctgctta gccatcaccg gtacgaaggt cgcgggaatg atgtaaccga
     1441 agccgaatgc gccatagcag aaaacgagct gcaacatccg cggagtccat tgccggcggc
     1501 ccgcgccggt gcgcccgcgt ttggtcggcg ccgcgccttc gtccactaca ggccatatca
     1561 gcgctgcaac cacgagggaa accatgccaa ggctcgccca ggccagccac gaacttgccc
     1621 gtatgctcat gagagcgagg cacatgccgc cggcaagggc gataccggag cccacgccgg
     1681 tgaagaccac gccaatcagg agcggcctgc gcagcgaggt gagccgctcc aaacaccagg
     1741 ccgacacatg gatcagcgcc cacgcgctcg cgaccccggc gagcatcctg aggacaagcc
     1801 aggcggcgaa ctgctcgacg agacccatgg cgagcgtggc gagcccgatg accagcaatg
     1861 cagcgcggat agcgagtggc gagcgcacac gatgcacggt cgcccaaagt gcaccgagga
     1921 agtagccaac gtagttcgcc gatgcgagcc agcttccccg ggcaatggac agcccggcat
     1981 cgtcctgcat catcggcaga atcggagtga aggcgaagcg gccgatgccc atggcgaccg
     2041 cgagcgcgat caaaccggcc gcggcaacgc tcgccgcaga ttcacgcggc tgccagctgt
     2101 cgagcgccgg acgcaaggag gccttcggcc tcgcgcatga tgcggtccac gatctcgcgt
     2161 gcgggaagga tgtcgcgcac caacccgcaa gcctcgccgg cgatgaccgg cgcaatgtcg
     2221 aagtcgtgct tgcggcgggc ttcggcgaga cgcgcggctt cctgatccac acgttgcatg
     2281 agctcgacct cgcgcccata ccagcgctcg acataggggt tgcgcatgca ccgcccggta
     2341 aacggcgccg gccagacgtt gcggcgcgaa atgtcgaaca cgatgctgcg c
//
LOCUS       DASUNT010000523         3559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1298888, whole genome shotgun sequence.
ACCESSION   DASUNT010000523 DASUNT010000000
VERSION     DASUNT010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298888"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VFX94_10565"
     CDS             <1..502
                     /locus_tag="VFX94_10565"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX5768667.1"
                     /translation="LWGVGPVTAGKLRKVGIERLVQIRDADIELLRDTVGSLAGWLRQ
                     LSHGDDPRPVVPDRESKSSSTENTYAQDLLDAGAIRAELDRMARENARWLVRRQLAAR
                     TVTIKVRYSDFKTVTRSHTLSRHTADADLIASWAVELAARTEAGAKPVRLLGVRVQGL
                     VEQAAG"
     gene            complement(479..1060)
                     /locus_tag="VFX94_10570"
     CDS             complement(479..1060)
                     /locus_tag="VFX94_10570"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX5768668.1"
                     /translation="MCGRYALHANPEVVALQFGLQSVPAFQPSYNIAPAAEILVVRPG
                     AAARARWGLRGRFVNLRAQTVLARFKASGRCLVPASGFYEWKAEGRRKQPYYFSPRSD
                     ALLALAAVWERDTCSLVTTEPDRVVGAVHERMPLIVPRSLYQAWLHGDEALLESPPHI
                     ELRAHPVGMAVNQAANDSPRLIEPVELARGLFD"
     gene            1087..2271
                     /gene="pncB"
                     /locus_tag="VFX94_10575"
     CDS             1087..2271
                     /gene="pncB"
                     /locus_tag="VFX94_10575"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX5768669.1"
                     /translation="MIIQSLLDTDLYKFTMMQVVLHHFPGAQVEYRFRCRTEGADLRP
                     NLDGIERELAELCRLRFRDEELAYLRSLRFMKSDFVDFLALFQFNEKYVHTGLGEKPG
                     EIDITIHGPWLHTILYEIPVLAIVSELYFRRTRPRPNLDEGRRRLKAKIDLVRTVEPE
                     LDFKISDFGTRRRFSLAWHEEVVQTLKREVPQFFAGTSNVWFAMRNNLTPLGTMAHEY
                     MQACQALGPRLRDSQTFAFDKWAQEYRGDLGIAIADTYGLEAFLRDFDMYFCKLFDGA
                     RHDSGDPFEWGEKMIAHYRANRVDPRTKTLIFSDQLSFPLAIEIARRFHGRARTSFGI
                     GTNLTNDLGYEPINIVLKMTECNGQPVAKVSDSPGKTISKDPGYLRYLRQVFGLEPAE
                     KI"
     gene            2285..3469
                     /locus_tag="VFX94_10580"
     CDS             2285..3469
                     /locus_tag="VFX94_10580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5768670.1"
                     /translation="MLAACFALNMFGRGLGDTYAVFLLPLEREFGWTRSQLTSVYSIY
                     LLVNGCTAPLVGLVFDRLGPRWVYGAGMTSLGAAFFLASGLTALWQFYLFIGVLVGIG
                     VSMNGMVPASALLGRWYRERLSSAIGIAYAAFGLGAVVFVPLVQYLVGEFGWRASYRI
                     MGSVLLALAPLVVIALPWRAFAAGHPASRRQARGKQAVGWTLAAAVRTPVFRGLVQVF
                     ACTAAAMFSIVVQLVAFLVDAGFSPLTAASAFGVVGLLSAASVTGSGFASDRFGYRQT
                     VTASFAGTAAGMLALLALTAVPSSALLIVFVAVFGLCMGVRGPIVSSVSARYFAGPRV
                     ATIYGLIYASNAIGAAVGSFAGGLLHDLTGGYRAGLAMALALIALAALPFWTVPGLRN
                     FR"
BASE COUNT          522 a         1230 c         1178 g          629 t
ORIGIN      
        1 gctctggggc gtggggccgg tgaccgccgg caagctgcgc aaagtcggaa tcgagcgcct
       61 cgtgcagatc cgcgacgccg acatcgaact tctgcgcgat accgtgggca gcctcgccgg
      121 ctggctgcgt cagctttccc acggcgacga tccccggccg gtcgtgccgg accgcgagtc
      181 caagtcttcc agcaccgaga atacctacgc gcaggacctc ctcgacgcgg gcgccattcg
      241 ggccgagctc gatcgcatgg cgcgcgagaa tgcccggtgg ctcgtgcgcc ggcagctcgc
      301 ggcgcgcacg gtgaccatca aggtgcgcta ttcggatttc aagacagtga cgcgcagcca
      361 tacgctgtcg cggcacacgg cggacgccga tctgatcgcc tcgtgggcgg tggagctggc
      421 ggcgcgcacc gaggccggag cgaagccggt gcggctcctc ggcgtgcggg tgcagggact
      481 agtcgaacag gccgcgggct agctcgacgg gctcgatcag gcgcggcgaa tcgttcgccg
      541 cctggttcac cgccatgccg accggatgcg cgcgcagctc gatgtgcggg ggtgactcga
      601 gcagcgcttc atcaccgtgc agccacgcct ggtagagtga cctgggcacg atgagcggca
      661 tgcgctcgtg cactgcgccc accacgcggt cgggctcggt agtgacgagc gagcaggtat
      721 cgcgctccca caccgcggcg agcgcgagca gagcatcgct gcgcggcgag aagtaataag
      781 gctgcttgcg ccggccttcg gccttccatt cgtagaagcc gctcgccgga acaaggcagc
      841 ggccgctcgc cttgaaccgc gcaagcacgg tctgcgcgcg caggttcacg aaccggccgc
      901 gcaggcccca gcgcgcgcgc gccgccgcgc cgggtctcac gacgaggatt tcggcggcgg
      961 gcgcaatgtt gtagctcggc tggaatgcgg ggaccgattg gaggccgaac tgcagcgcca
     1021 ccacctcggg attcgcatgc agcgcgtaac ggccgcacat gagcccaaga gtacaatttc
     1081 cgcgacatga tcatccagtc gctgctcgac accgacctgt acaagttcac catgatgcag
     1141 gtcgtgctgc accattttcc gggcgcccag gtggagtacc ggttcaggtg ccgcaccgag
     1201 ggcgcagacc tgcgtcccaa cctcgacggc atcgagcgcg agctcgcgga gctctgtcgc
     1261 ctgcgcttcc gcgacgaaga gctcgcgtac ctgcgctcgc tgcgcttcat gaagtcggac
     1321 tttgtcgact tcctcgcgct tttccagttc aacgaaaaat acgtgcacac gggcctgggc
     1381 gaaaagccgg gcgagatcga catcaccatc cacggcccgt ggctgcatac catcctctac
     1441 gagatcccgg tgctcgcgat cgtgtcggag ctttacttcc ggcgcacccg gccgcgcccg
     1501 aacctcgacg aggggcggcg gcgcctgaag gcgaagatcg acctcgtgcg caccgtagag
     1561 cccgagctcg acttcaagat ttccgacttc ggcacgcgcc ggcgcttctc gctcgcctgg
     1621 cacgaggagg tcgtgcagac gctcaagcgc gaggtgccgc agttcttcgc cggcacctcg
     1681 aacgtgtggt tcgccatgcg caacaacctc actccgctcg gcaccatggc gcacgagtac
     1741 atgcaggcgt gccaggccct cggcccgcgg ctgcgcgact cgcagacttt cgccttcgac
     1801 aagtgggcgc aggaataccg cggcgacctc ggcatcgcca tcgccgacac ctacgggctg
     1861 gaggcgttcc tgcgggattt cgacatgtac ttctgcaagc tgttcgacgg ggcgcggcac
     1921 gactcgggcg atcccttcga gtggggcgag aaaatgatcg cgcattaccg cgcgaaccgg
     1981 gtcgacccgc gcaccaagac gctcatcttc tccgaccagc tgtccttccc gctcgcgatc
     2041 gagatcgcgc ggcgattcca cggccgcgcg cgcacctcct tcggcatcgg caccaacctc
     2101 accaacgacc tcggctacga gcccatcaac atcgtgctca agatgaccga gtgcaacggc
     2161 cagccggtcg ccaaggtttc cgactctccc ggcaagacca tcagcaagga cccgggctac
     2221 ctgcgctacc tgcggcaggt cttcggcctc gagcctgccg agaagatcta gcaccgcggc
     2281 cgcggtgctc gcggcctgct tcgccctcaa catgttcgga cgcgggctcg gcgataccta
     2341 cgccgtgttc ctgctcccgc tcgagcgcga gttcgggtgg acgcgctcgc agctcaccag
     2401 cgtctattcg atctaccttc tcgtgaacgg ctgcacggcg ccgctcgtgg gcctcgtgtt
     2461 cgaccggctc gggccgcgct gggtctacgg cgccggaatg acgagcctcg gcgcggcctt
     2521 cttcctcgcg agcgggctca ccgcgctgtg gcagttctat ctcttcatcg gcgtgctggt
     2581 gggcatcggg gtcagcatga acggcatggt tccggcctcg gcgctcctcg gccgctggta
     2641 tcgcgagcgg ctctcgagcg cgatcgggat cgcgtatgcc gccttcgggc tcggcgcggt
     2701 ggttttcgtc cctctggtgc agtacctcgt gggcgagttc gggtggcgcg caagctaccg
     2761 gatcatgggc tcggtactgc tcgctcttgc gccgcttgtc gtgatcgcgc tcccctggcg
     2821 cgccttcgcc gcgggacacc cggcgtcgcg ccgccaggcg agaggaaagc aagccgtggg
     2881 atggacgctc gcggcggcgg tgcgcacccc ggtgttccgg gggctcgtcc aggtgttcgc
     2941 ctgcaccgcg gccgccatgt tctcgatcgt ggttcagctg gtcgcgtttc tcgtcgatgc
     3001 ggggttttca ccgctcactg cggcgagcgc gttcggcgtc gtcgggctgc tctcggcggc
     3061 gagcgtgacg ggaagcggct tcgcctcgga tcgcttcggc taccggcaga ccgtcaccgc
     3121 cagctttgcc ggcacggcag cgggaatgct cgcgctcctt gcgctgacgg cggtcccttc
     3181 gagcgccttg ctgatcgtct tcgtggcggt cttcgggctt tgcatgggag tgcgcgggcc
     3241 gatcgtctct tccgtatcgg cccgctattt cgccggcccg cgggtggcga ccatctatgg
     3301 tttgatctat gccagcaacg cgatcggcgc cgccgtcggc tcgttcgccg gcggcctgct
     3361 gcacgatctc accggcggct atcgcgccgg cctcgccatg gcgctcgcgc tgatcgcgct
     3421 cgctgcgttg ccgttctgga cggtgccggg gctcaggaat ttccgctgag ccgagactgt
     3481 ccccggttct cgagcgccgt ctccaagctt gtcaagggcg cctcgggagt cctaaactcg
     3541 gcttctggca atcttcgac
//
LOCUS       DASUNT010000524         2473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_335086, whole genome shotgun sequence.
ACCESSION   DASUNT010000524 DASUNT010000000
VERSION     DASUNT010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335086"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VFX94_10585"
     CDS             complement(<1..469)
                     /locus_tag="VFX94_10585"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX5768671.1"
                     /translation="MKIIILGAGQVGRTAAHHLSREEANEVTVVDTNDEALRDLQDRL
                     DIRTVSGNAASPSVLEAAGVADADIIIALTSSDEVNMLACQVAYVLYRTPTKIARIRS
                     ADYYGREGLFAESGLAVDTWISPEQLVTDYIARLIRLPGALQVLDFADGKVRLV"
     gene            complement(466..>2473)
                     /gene="uvrD"
                     /locus_tag="VFX94_10590"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(466..>2473)
                     /gene="uvrD"
                     /locus_tag="VFX94_10590"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEX5768672.1"
                     /translation="GASPHSILAVTFTNKAAGEMRGRIESLLGIPGSALWVGTFHGLA
                     HRLLRLQWREAGLKQDFQILDAEDQVRTVRKVVKALDLDENRWVPREIAWFINAQKDE
                     GHRPKNLKDNGDPTRREYIRLYQAYEDACARAGVVDFAELLLRAYEVWRDWPELLAHY
                     RRRFRHILVDEFQDTNAIQYAWLRLLAGDEGLPFVVGDDDQSIYRWRGARVENLQQYQ
                     RDFAGAKLYRLEQNYRSTGTILKAANALIANNSGRLGKNLWTSGEQGAQIRLYAAFNE
                     RDEADFVVNRIREWVRRGNARREIAILYRSNAQSRVFEEAFLSAAIPYRVYGGLRFFE
                     RAEIKDALAYLRLTQSRDDDSSFERVVNLPARGIGAKSLDAVREYARANASSLWVAAG
                     ACMKDSLGPKAGAAMHAFMVLIERLAHEIRALELHEQVDHVINSSGLLDHHKREKGDR
                     GETRVDNLLELVSAARGFTQEPGSELAPLNAFLAHAALEAGEGQAQAWEDCVQMMTLH
                     SAKGLEFPVVFLCGMEDGLFPHQRSLNDLQGLEEERRLCYVGMTRAMQELVLTYAEQR
                     RLHGTDSFGQVSRFVNEVPEELIEEVRPRIQISRPVHFNRGGRLVEESDSGMRLGQRV
                     RHGKFGEGVVMNIEGQGANARIWVNFEALGTKCLMAGYAKLEPM"
BASE COUNT          385 a          845 c          811 g          432 t
ORIGIN      
        1 cgaccaggcg gaccttgccg tcggcgaaat ccagcacctg cagggcgccg ggcaggcgga
       61 tgaggcgtgc aatgtagtcg gtaaccagct gttcgggact gatccacgtg tcgaccgcca
      121 ggccgctctc cgcgaacagg ccctcgcgcc cgtaatagtc ggcggagcgg atgcgcgcga
      181 tcttcgtggg cgtgcggtag agcacgtagg cgacctggca ggccagcatg ttcacttcgt
      241 cgctggaggt gagcgcgatg atgatgtcgg catccgcgac gcccgcggcc tcgagcaccg
      301 agggactggc tgcgttgccg ctcacggtgc ggatgtccag gcggtcctgc aggtcgcgca
      361 gcgcctcgtc gttcgtatcg acgaccgtga cctcattcgc ctcctcgcgc gaaaggtggt
      421 gcgcagcggt gcgcccgacc tggcccgcgc cgaggatgat gatcttcaca tgggctccag
      481 cttggcgtag cccgccatca ggcacttggt gccgagcgcc tcgaaattga cccagatgcg
      541 cgcgttcgcc ccctggcctt cgatgttcat gaccacgcct tctccgaatt tgccgtggcg
      601 cacgcgctgc ccgaggcgca tgccggagtc gctttcttcg accaggcgcc cgccgcgatt
      661 gaaatgcacc gggcgcgaga tctggatgcg cgggcgcact tcctcgatca gctcctcggg
      721 aacctcgttc acgaaacgcg aaacctggcc gaacgaatcc gtgccgtgca ggcgccgctg
      781 ctcggcgtag gtgagcacga gctcctgcat cgcacgcgtc atgcccacgt agcacaggcg
      841 ccgctcttcc tcgaggccct gcaggtcgtt gagcgagcgc tggtgcggga aaaggccgtc
      901 ttccatgccg cacaggaaca cgacagggaa ttccaggccc ttggccgaat gcagcgtcat
      961 catctgcacg cagtcttccc aggcctgcgc ctggccttcg ccggcctcga gcgctgcatg
     1021 cgccaggaag gcgttcagcg gcgcgagctc gctgcccggc tcctgcgtga agccgcgcgc
     1081 ggcgctcacg agctcgagca ggttgtcgac gcgcgtctcg ccgcgatcgc ccttctcgcg
     1141 cttgtggtga tcgagcagac cactcgagtt gatgacgtga tcgacctgct cgtgcagctc
     1201 gagcgcgcgg atctcgtgcg ccagccgctc gatgagcacc atgaatgcgt gcatcgccgc
     1261 accggccttg gggccgagcg aatccttcat gcaggcgccg gccgccaccc agagcgagct
     1321 ggcgttggcg cgcgcgtact cgcgcacggc atccaggctc ttcgcgccga tgccgcgcgc
     1381 gggcaggttc accacgcgct cgaacgagct gtcgtcgtcg cggctctgcg tgaggcgcag
     1441 gtaggcgagc gcgtccttga tttcggcgcg ctcgaagaat cgcaggccgc cgtacacgcg
     1501 atacggaatg gcggccgaca ggaacgcctc ttcgaacaca cgcgactgcg cgttggagcg
     1561 atacaggatc gcgatctcgc gccgcgcgtt gccgcgcctc acccattcgc gaatgcggtt
     1621 gacgacgaag tccgcctcgt cgcgctcgtt gaacgccgcg tacagacgga tctgcgcgcc
     1681 ctgctcgccg ctggtccaca gattcttgcc gagccggccg ctgttgttcg cgatcagcgc
     1741 gttcgcggcc ttgaggatcg tgccggtgga gcggtagttc tgctccaggc gatacagctt
     1801 cgcgccggca aaatcgcgct ggtattgctg caggttctcg acgcgagcgc ctcgccagcg
     1861 gtagatgctc tgatcgtcgt cgcccacgac gaagggcagg ccttcgtcgc ccgcgagcag
     1921 cctgagccac gcatactgga tcgcattggt gtcctggaac tcatccacca ggatgtggcg
     1981 gaagcgcctg cgatagtgag cgagcaactc cggccagtcg cgccacactt cgtacgcgcg
     2041 cagcagcagc tcggcgaaat ccaccacacc ggcgcgcgcg caggcgtcct cgtaggcctg
     2101 gtaaagccgg atgtactccc gccgcgtggg atcgccgttg tccttgaggt tcttcggcct
     2161 gtggccctcg tccttctgcg cgttgatgaa ccacgcgatc tcgcgcggca cccagcggtt
     2221 ctcgtccagg tcgagcgcct tgaccacctt gcgcacggtg cgcacctggt cctcggcgtc
     2281 gaggatctga aaatcctgtt tcaggcccgc ctcgcgccac tgcagccgca gcaggcggtg
     2341 cgcgaggcca tggaacgtgc ccacccacag tgccgagccc ggaatgccga gcagcgattc
     2401 gatgcgcccg cgcatttcac ccgccgcctt gttggtgaaa gtgacggcga gaatgctgtg
     2461 cggcgaggcg cct
//
LOCUS       DASUNT010000525         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_946430, whole genome shotgun sequence.
ACCESSION   DASUNT010000525 DASUNT010000000
VERSION     DASUNT010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946430"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1162
                     /locus_tag="VFX94_10595"
     CDS             173..1162
                     /locus_tag="VFX94_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5768673.1"
                     /translation="MNGRSTSYVVSGVVLLILIALAFAVGRYPVSLSELFSVLSGQPV
                     AANVETVVLQVRGPRVLAALLVGAALAAAGTAYQGMFRNPLVSPDILGVSTGAALGAV
                     LAIFLSQNVFMTQMLAFGGGLAAVGLVYGVGSRLRGHDPLLALVLTGVVIGTLLGSAI
                     ALLKYLADPYNQLPAITFWLLGSLASIAPKDLLVAAPLAAAGLVPMLLLRWRMNLLAL
                     PDDEARALGVDTRRLRTLVVACATLTTASVVAISGIIGWVGLLIPHAARLLVGPDFGR
                     LLPLAILMGAAFMLAVDTLCRTIASIEVPPGVLTALIGTPFFLWLFALARRTW"
     gene            1162..1932
                     /locus_tag="VFX94_10600"
     CDS             1162..1932
                     /locus_tag="VFX94_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5768674.1"
                     /translation="MLEVRELAFGFPGRTVGRDVSFTLAAGEVMCVLGPNGGGKTTLF
                     RTLLRLLEPHAGKIFVDSFSLESLSRAEIARRIGYVPQGHSAYFAFTVREFVLMGRTA
                     HLGAFASPAKSDVRVAERSLESLGIAHLADKPVTEISGGERQLALVARALAQEPNLMV
                     LDEPTASLDFGNQVRVLEKISALARSGIAVLFSSHDPDHAFLCANRALLLAEGRVLEA
                     GTPREVIRADTLERMYRVKVRVVPLPGGMHTCLPGMRP"
BASE COUNT          284 a          768 c          703 g          396 t
ORIGIN      
        1 cagcgcgctg aaggtcctgc tcgagatgct gcagggagcc gggtttttgg cagccgcggc
       61 gcgcttgccg ggttacgacg cctcgcgtgg cggccaactc gagccactgg acgcggcgct
      121 cacctgggta gaacagccgc gccgcgccac gcatggctga gagcacgccg aagtgaacgg
      181 ccgcagcact tcctatgtcg tctcaggcgt cgtgctgctg atcctgattg cgctcgcctt
      241 cgcggtcggc cgctatccgg tgagcctcag cgagcttttc tcggtccttt caggccagcc
      301 ggtggcggcc aatgtcgaga cggtggtcct gcaggtgcgc gggccgcgcg tgcttgccgc
      361 tcttctcgtc ggagccgcgc tcgccgcggc cggcaccgcc tatcagggga tgttccgcaa
      421 ccccctcgtc tcgcccgaca tcctcggcgt gtccaccggc gccgcgctcg gcgcggtgct
      481 ggcgatcttt ctttcccaga acgtcttcat gacccagatg ctcgcattcg ggggcggcct
      541 ggccgccgtc ggcctcgtct acggcgtcgg ctcgagactg cgcggccatg acccgctcct
      601 tgcactggtg ctcaccggcg tcgtcatcgg caccctgctc ggctcggcga tcgcgcttct
      661 caagtacctt gcggacccct acaaccagct acccgcgatc acgttctggc tgctcggcag
      721 cctcgcctcc atcgcgccga aggacctgct cgtggccgcg ccgctcgcgg ctgccgggct
      781 cgtaccgatg ctgcttctgc gctggcgcat gaacctgctc gcgctgcccg acgacgaagc
      841 gcgcgcgctc ggcgtggaca cgcggcgcct gcgcacgctg gtcgtggcct gcgccacgct
      901 cacgaccgcg tccgtggtgg cgatcagcgg catcatcggc tgggtaggcc tcctcatccc
      961 gcacgccgcg cgcctcctcg tcggtcccga ctttggccgg ctgctgccgc tggcgatcct
     1021 gatgggcgcg gcgttcatgc tcgcggtcga tacgctatgc cgcaccatcg cctcgatcga
     1081 ggtcccgccg ggcgtgctca cggcgctcat cggcacgccg ttcttcctct ggctcttcgc
     1141 actggcgcga agaacgtggt gatgctcgaa gtgcgcgagc tcgctttcgg ctttcccggc
     1201 cgcacggtcg gccgcgacgt gagcttcacg ctcgccgccg gcgaggtgat gtgcgtgctc
     1261 ggccccaacg gcggcggcaa gactacgctt ttcaggaccc tcttgcggtt gctcgagccg
     1321 cacgccggaa agattttcgt tgattccttt tctttggaaa gcctctcccg agccgagatt
     1381 gccaggcgca tcgggtacgt gccgcaggga cattcggcct acttcgcctt cacggtgcgg
     1441 gaattcgtgc tgatgggccg aaccgcgcac ctcggggcgt tcgcttcccc cgcgaagagc
     1501 gacgtgcggg tcgcggagcg ctcgctcgaa tcgctcggca tcgcgcatct cgcggacaag
     1561 ccggtgaccg agatctcggg cggcgagcgc cagctcgcgc tcgtggcgcg cgccctggcg
     1621 caggagccga atctgatggt cctggacgag cccaccgcga gcctcgactt cggcaaccag
     1681 gtgcgcgtgc tggagaagat ctcggcgctc gcgcgctccg gcatcgcggt gcttttctcg
     1741 agccacgacc cggaccatgc gttcctctgc gcgaaccgcg cgctgctcct cgcggaaggc
     1801 cgcgtgctgg aagcgggcac gccgcgcgaa gtcatacgcg ctgacacgct tgagcgcatg
     1861 taccgcgtca aggtgcgcgt ggtcccgctt cccggcggca tgcatacgtg cttgccgggc
     1921 atgcggcctt gatctagact tttcgcacgt cggatgaaac gacaccgtcg gggctgcaga
     1981 ctcccggcga tccaaggcgc ctcgcgtcca agctctgcgg catgctcttg aaaggagacg
     2041 ccatggacgc gagcatcagc gcagcattcc tcaggcaatc catcgacggc accgagccgc
     2101 cgctcgtcat tgacgtgcgg cgcaacgccc ggtttttcga agccggagag c
//
LOCUS       DASUNT010000526         3920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1034394, whole genome shotgun sequence.
ACCESSION   DASUNT010000526 DASUNT010000000
VERSION     DASUNT010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034394"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2159)
                     /locus_tag="VFX94_10605"
     CDS             complement(<1..2159)
                     /locus_tag="VFX94_10605"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5768675.1"
                     /translation="MPTARLRSALSRIPLGRALIALGLVLVGINIGSAIWDVRSAYDR
                     TERRATRYYANMTRLLAEQTAASMEAVDLILRDAARTGRSHAVADGLMRVGEELLRVP
                     QIGAMLVLDSKGEVLARSSQTPAFDPDLAERPFFAAHRDGAPDLLHLSAPYRVAPGGQ
                     WRFVLSRRLSNEGAFDGVIAAAIEVEAFDRLYRTIDLDEGGFITLIAINGTLLTRIPD
                     PGIARGRRLPGGAIMAAVDREGRFEGWTTSPISGERVLLAAHAVRGFPLLVASGSNEA
                     AVFAPWREEAWLVFDRTLLTSAAMLALIGLAAWGLARRERALARSWRRYQSMIEHSSD
                     ALILSRPTKGGILYASPAIERLLGYRRDELQGREVMDYIHAEHLEAAMKARADLLRAP
                     GKVAVDEVKMVHKDGSWRWIEITRSNLLEEPSVRAVVFNFRDITERKQAEAERARLES
                     RLRQAEKLEAVGRLAGGIAHDFNNILGGILGYAEMLAENAPEGSALKRYADNVLSGAN
                     RASGLVEQILSYSRSQRGKRAPVELGRIVAETLELVRGSLPRGITLEVESPREELFVV
                     GDATQLHQVTMNLCTNACHAMEKGGVLKVVLDHESLAADRTLAHSTLHAGNYARLTVS
                     DTGSGMDEATLARIFEPFFTTKPVGQGTGLGLSVVHGIVRDHGGVVRATSTLGAGSMF
                     EVYFPAADGEAKREPIPVVSALPKSKRCLLFVDDEDSL"
     gene            complement(2170..2562)
                     /locus_tag="VFX94_10610"
     CDS             complement(2170..2562)
                     /locus_tag="VFX94_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5768676.1"
                     /translation="MSDKEKPREILVIDDDRIMRDLMTDWLEAAGYTVHKATDCNAAC
                     VAMDRAPALVVSDMWMPGPCGTAAIAFLKNKCSETKLIAVSGHFGSGQGTSEQDALAA
                     GAARALAKPVKRAELLAAVAELIGPPAK"
     gene            2677..3426
                     /locus_tag="VFX94_10615"
     CDS             2677..3426
                     /locus_tag="VFX94_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5768677.1"
                     /translation="MSVQPPASPQVALPHVLAVDDDPTIRGLIAEYLGQHDLRVTTVA
                     DGSAMREVMSQQVVDLIVLDLKLRTEDGMALARALRDESAIPIIMLTGRSEEADRVMG
                     LELGADDYLTKPFSPRELLARIRTILRRRRAEVRQGRPEGIRAYRFDGWELNLNTRRL
                     AAPDGRNVPLSNGEFSLLVVLLGSPQRILTRDQLIDLSRLHNDEVYNRSVDVQIMRLR
                     RKIEKDPAEPRYIRTERGAGYLFGMPVETVY"
     gene            3423..3797
                     /locus_tag="VFX94_10620"
     CDS             3423..3797
                     /locus_tag="VFX94_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006414039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5768678.1"
                     /translation="MIALNHTIVSARDKRASAAFLTEILGLQKPPVAFGPFMCVQLGN
                     DVTLDFCDDEGEIVSQHYAFLVSEKEFDQIFGRIQQRKLDYWGDPAREKPGEVYTWNG
                     GRGVYFLDPSGHFLEIHTRPYF"
BASE COUNT          607 a         1376 c         1305 g          632 t
ORIGIN      
        1 agcgagtctt cgtcgtcgac gaacagaagg cagcgcttgc tcttcggcag agcgctcacg
       61 accgggatgg gctcgcgctt ggcttcgccg tcggcggccg gaaagtacac ctcgaacatc
      121 gagcccgcgc cgagcgtact ggtcgcgcgc acgacgccgc cgtgatcgcg cacgatgccg
      181 tgcacgaccg aaaggccgag gcccgtgccc tgaccgacgg gcttggtagt gaagaacggc
      241 tcgaagatgc gcgcgagcgt tgcctcgtcc atcccgctgc ccgtgtcgct caccgtgagg
      301 cgcgcgtagt ttcccgcgtg cagcgtgctg tgggcgagcg tgcggtccgc cgccaggctc
      361 tcgtggtcga ggactacctt cagcacgccg cctttctcca tggcgtggca ggcgttggtg
      421 cagaggttca tcgtgacctg gtgcagctgc gtggcatcgc cgacaacgaa aagctcctcg
      481 cgcggcgact cgacttcgag cgtaatgccg cgcggcagcg agccgcgcac cagctcgagc
      541 gtttcagcga cgatgcggcc gagctccacc ggcgcgcgct tgccgcgctg gctgcggcta
      601 taggagagga tctgctccac cagcccgctc gcgcggttgg cgcccgaaag cacgttgtcc
      661 gcgtagcgct tgagcgccga gccttcgggc gcgttctcgg cgagcatctc ggcatagccg
      721 aggatcccgc ccaggatgtt gttgaagtcg tgcgcgatgc cgcccgcgag ccgcccgacc
      781 gcctcgagct tctccgcctg gcgcagccgg ctctcgagcc gcgcgcgctc ggcctcggcc
      841 tgcttgcgct cggtgatgtc gcggaagttg aacaccacgg cgcgcacgct gggctcctcg
      901 agcaggttgg accgcgtgat ctcgatccag cgccacgagc cgtccttgtg caccatcttc
      961 acttcgtcca ccgccacctt gccgggcgcg cgcagcaggt cggcgcgcgc cttcatcgcc
     1021 gcctcgagat gctccgcgtg gatgtagtcc atcacttcgc gaccctgcag ctcgtcccgg
     1081 cggtagccga gcagtcgctc gatcgcggga ctcgcgtaga ggatgccgcc cttggtaggg
     1141 cgcgacagga tgagcgcatc cgagctgtgc tcgatcatcg actggtagcg ccgccagctg
     1201 cgcgcgagcg cgcgctcgcg gcgcgcgagt ccccaggccg cgagaccgat gagcgccagc
     1261 atcgccgccg aggtaagcag cgttcggtcg aacacgagcc acgcctcctc gcgccacggc
     1321 gcgaacaccg cggcctcgtt gctgccgctc gccacgagca gcgggaagcc gcgcaccgcg
     1381 tgcgccgcga ggagcacccg ctcgccgctg atcgggctcg tggtccaccc ttcgaagcgg
     1441 ccctcgcggt ccaccgcggc catgatcgcg ccgcctggaa gcctgcggcc gcgggcgatg
     1501 ccggggtcgg ggatgcgtgt gagcagcgtg ccgttaatcg cgatgagcgt gataaagccg
     1561 ccttcgtcga ggtcgatcgt gcggtagagg cggtcgaagg cctcgacctc gatcgcggcg
     1621 gcgatgacgc cgtcgaacgc tccttcgttg ctcaggcgcc gcgacagcac gaagcgccat
     1681 tgcccgccgg gggccacgcg gtacggcgcg ctgagatgga gcaggtccgg cgcgccgtcg
     1741 cggtgcgcgg cgaaaaacgg ccgctcggcg agatccggat cgaacgccgg cgtctggctc
     1801 gagcgcgcga gcacctcgcc cttcgagtcg agcaccagca tggcgccgat ctgcggcacg
     1861 cgcagcagct cctcgccgac tcgcatcagc ccgtcggcga ccgcgtgcga acggccggtg
     1921 cgcgccgcat cgcgcagtat caggtccacg gcttccatgg aggccgcggt ctgctcggcg
     1981 agcagccgcg tcatgttggc gtagtagcgc gtcgcgcggc gctcggtgcg gtcgtaggcc
     2041 gagcgcacgt cccagatggc cgagccgatg ttgatgccga cgagcaccag gccgagggcg
     2101 atcagcgcgc ggccaagggg tatccgggag agtgccgagc ggaggcgggc cgtcggcatg
     2161 gcgtccttct tacttcgcag gcggcccaat cagctcggcg acggccgcga ggagctcggc
     2221 gcgcttgacc ggcttggcga gcgcccgcgc ggcgccggcg gcgagcgcgt cctgctcgct
     2281 cgtgccctga cccgagccga aatgcccgga cacggcaatc aactttgttt cagaacactt
     2341 gttcttcaga aaagcgatgg ctgccgtgcc gcaagggccg ggcatccaca tgtcgctcac
     2401 gacgagggcc ggcgcgcgat ccatggctac gcaggcggcg ttgcagtcgg tggccttatg
     2461 gacggtgtag cctgccgcct ccagccagtc ggtcatcagg tcgcgcatga tccggtcgtc
     2521 gtcgatgacc aggatctcgc ggggtttttc cttgtcgctc aagcgcccat ttcacccgat
     2581 gccaattgcg tgtaatttac atccggcgct gccgcaggtt acagatgtaa cggcgcacgg
     2641 cggcgcctga ctagacttga cggcttgcgc gaaacgatga gtgttcaacc cccggcatcc
     2701 ccgcaagtgg cgctcccgca cgtgctcgcg gtggacgacg acccgaccat ccgcggcctg
     2761 atcgcggaat acctcgggca gcacgacttg cgggtcacca cggtcgccga cggatcggcc
     2821 atgcgcgagg tcatgtcgca gcaggtggtc gatctgatcg tcctcgacct caagctcagg
     2881 accgaggatg gcatggcgct cgcgcgcgcg ctgcgcgacg agtcggcgat tccgatcatc
     2941 atgctcaccg gccgctcgga ggaagccgac cgggtaatgg gcctcgagct cggcgccgac
     3001 gactacctca ccaagccctt cagcccacgc gagcttctcg cccgcatccg caccatcctg
     3061 cgccgccgcc gcgccgaggt tcgccagggg cggcccgagg gcatccgcgc ctaccgcttc
     3121 gacggctggg agctcaacct gaacacccgt cgcctcgccg cgcccgacgg ccgcaacgtg
     3181 ccgctctcga acggcgaatt cagcctgctc gtcgtgctgc tcggctcgcc gcagcgcatc
     3241 ctcacgcgcg accagctgat cgacctctcg cgcctgcaca acgacgaggt ctacaaccgc
     3301 tcggtcgacg tgcagatcat gcgcctgcgc cgcaagattg aaaaagaccc ggccgagccg
     3361 cgctacatcc gcaccgagcg gggcgcgggc tacctcttcg gcatgccggt ggaaacggtc
     3421 tattgatcgc tctcaaccac actatagtct ccgcgcgcga caagcgcgcc tcggcggcct
     3481 tccttacgga aatcctcggc ctgcaaaagc cgccggtcgc tttcggcccg ttcatgtgcg
     3541 tgcagctcgg caacgacgtg acgctcgact tctgcgacga cgaaggagag atcgtgtcgc
     3601 agcactacgc gttcctggta agcgaaaagg aattcgacca gatcttcggc cgcatacagc
     3661 agcgcaagct cgactactgg ggcgacccgg cgcgcgagaa gccgggcgag gtgtacacct
     3721 ggaacggcgg ccggggcgtc tactttctgg atccgtcagg ccacttcctc gagatccaca
     3781 cccgccccta cttctagcct tcggcccact cccgtcccac ccgggctctt cattttttcg
     3841 tcccaccggc gtatagaggg tgggttattt ctggaggagg ccaagaatga ggcgcttctc
     3901 gctggcggcg ggcgcgatcg
//
LOCUS       DASUNT010000527         3108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_859562, whole genome shotgun sequence.
ACCESSION   DASUNT010000527 DASUNT010000000
VERSION     DASUNT010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859562"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VFX94_10625"
     CDS             complement(<1..506)
                     /locus_tag="VFX94_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5768679.1"
                     /translation="MLAYVVRRVLYAVPILIGVNLITFVLFFVVNTPDDMARMHLGAK
                     RVTPEAIEKWKRERGYEKPLLYNRSGSGLARITDTIFFDKSLKLFAFDFGRAEDQRDI
                     AHEIRSRMGPSLALAIPVFLVGLAVNITFALLMAFFRATYVDFWGVVLCVAMMSISTL
                     FYIIGGQYL"
     gene            complement(506..2605)
                     /locus_tag="VFX94_10630"
     CDS             complement(506..2605)
                     /locus_tag="VFX94_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5768680.1"
                     /translation="MRPIILSGVRILLMLVVCVAGCGEVWNDPYPRSERGANILYAAF
                     VQRPKHLDPVQSYTVDEALFTRQILEPPLQYHYLKRPYELIALTAAELPKPKEIEGGK
                     FTVYEIRIRPGIHYQPHPAFLEKNHGLTAEQIKTLETPYALPLGTRELVAADYVYQIK
                     RLAHPRLHSPIFGLMAEYIVGLPEFAEQLKKLNSDGWIDLRSHRLEGVEDVDRYTYRI
                     KLKGRYPQFVYWLAMPFFSPIPWEAEKFFHQPGMELRNFTLDWWPVGTGPYMLTENDP
                     NARMVLGRNPNYRGMAYPSEGEPGDAEAGLLRDAGNTMPFIERVVFSREKESVPYWNK
                     FIQGYYDSAGISAENFDQAVRVAVDGAAEPSPEMVERGIELTTSVAATVYYFGFNMKD
                     PVVGGDSERARKLRQAISIAVDMEEYLSIFLNGRGIVAHSPLPPGIFGAREGREALNP
                     VTHVWREGRESRRPLPEAKKLLAEAGYPDGRDAKTGAPLVLYFDTTARGPGDKARLDW
                     WRKQFDKLSIQLEVRETDWNRFQEKIRKGTQQVYGLGWHADYPDPENFLFLLHGPQAR
                     ADGAGSNSSNYTSPEFDALFERMKSMPNSPERQALIERMVDIARRDAPWIFGFHPKDY
                     TLRHRWLANVKPNDMAGNTMQYLRLDPTLRAELRAQWNRPVVWPALLLLAVLIASAVP
                     AFLTYRRRERMAARPAA"
     gene            2604..>3108
                     /locus_tag="VFX94_10635"
     CDS             2604..>3108
                     /locus_tag="VFX94_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5768681.1"
                     /translation="MALLAGKKIMVTGVLSNRSIAYGVAKVARREGAEIALTYQNERF
                     KDRVTEMAKELGADIALPCDVSVDREIQDLFSRLAENWQRLDGLVHAIAFAPREAIAG
                     DFLEGMSREAFRQAHDISSYSFAALAKGAMPLLQESKASLLTLTYLGAERVVPNYNTM
                     GLAKASLE"
BASE COUNT          525 a          978 c         1054 g          551 t
ORIGIN      
        1 aggtactgcc cgccgatgat gtagaacagc gtcgaaatgg acatcatcgc gacgcacagc
       61 accacgcccc agaaatcgac gtaggtggcg cggaagaacg ccatcagcag cgcgaacgtg
      121 atgttcaccg cgagcccgac caggaacacc gggatcgcca gcgcgaggct cgggcccatg
      181 cggctgcgga tctcgtgcgc gatgtcgcgc tggtcctcgg cgcggccgaa gtcgaaggcg
      241 aagagcttca gcgacttgtc gaagaagatg gtgtcggtga tcctggcaag gcccgatccg
      301 gaccggttat ataggagcgg cttttcatat cccctttccc gcttccattt ctcgatcgcc
      361 tcgggcgtga cgcgcttcgc gcccaggtgc atgcgcgcca tgtcgtccgg cgtgttcacc
      421 acgaagaaca gcacgaacgt aatgaggttc accccgatca ggatcgggac cgcgtagagc
      481 acgcgccgga cgacgtaggc gagcatcagg ccgcgggccg cgcggccatg cgttcacgcc
      541 gccggtaagt caggaatgcc ggaacggcgg aggcgatcaa tacggcgaga agcaggagcg
      601 caggccacac caccggccgg ttccactggg cgcgcagctc ggcgcgcagc gtcgggtcga
      661 ggcgcaggta ctgcatcgtg ttgccggcca tgtcgttcgg cttcacgttg gcgagccagc
      721 ggtggcgcag ggtgtagtcc ttagggtgga agccgaagat ccacggcgcg tcgcgccgcg
      781 cgatgtcgac catgcgctcg atgagcgcct gccgctcggg cgaattgggc atgctcttca
      841 tgcgctcgaa gagcgcgtcg aactccgggc tcgtgtagtt ggagctgttc gagcccgcgc
      901 cgtcggcgcg cgcctgcggg ccgtgcagga ggaacaggaa gttctccggg tcgggatagt
      961 cggcgtgcca gcccaggccg tagacctgct gcgtgccctt gcggatcttc tcctggaagc
     1021 ggttccagtc ggtctcgcgc acctcgagct ggatcgagag cttgtcgaac tgcttgcgcc
     1081 accagtctag gcgcgccttg tcgcccgggc cgcgcgcggt ggtgtcgaaa tacaggacga
     1141 gcggcgcgcc ggtcttggca tccctgccgt cggggtagcc ggcctcggcg agaagcttct
     1201 tggcctcggg aagcggcctg cggctctcgc ggccttcgcg ccacacgtgg gtgaccgggt
     1261 tcagggcctc gcgcccctcg cgcgcgccga agatgcccgg cggcagcggg ctgtgcgcca
     1321 cgatgccgcg gccgttcagg aagatcgaca ggtactcctc catgtcgacc gcgatcgaga
     1381 tcgcctggcg cagcttgcgc gcgcgttccg agtcgcctcc caccaccggg tccttcatgt
     1441 tgaacccgaa gtagtacacc gtcgcggcga ccgaggtggt gagctcgatg ccgcgctcga
     1501 ccatctccgg cgaaggctcg gcggcgccgt cgaccgcgac gcgcaccgcc tggtcgaagt
     1561 tttcggcgct gatgccggcg ctgtcgtagt agccctggat gaatttattc cagtacggga
     1621 cgctttcctt ttcgcggctg aaaaccacgc gctcgatgaa cggcatggtg ttccccgcgt
     1681 ctctcagcaa gccggcttcc gcgtcgccgg gctctccttc cgagggataa gccatgccgc
     1741 gatagttggg gttgcggccg agcaccatgc gcgcattggg atcgttctcg gtgagcatgt
     1801 acggaccggt tccgaccggc caccagtcga gggtgaagtt cctgagctcc atcccgggct
     1861 ggtgaaaaaa cttttccgct tcccatggaa tcggcgagaa gaaaggcatc gccagccagt
     1921 agacgaactg cgggtagcgg cccttgagct tgatgcggta ggtgtagcgg tcgacgtcct
     1981 cgacgccctc gagccggtgg ctgcgcaggt cgatccatcc gtcggagtta agcttcttca
     2041 gctgctccgc gaattccgga agaccgacga tgtactccgc catgagcccg aagatcggcg
     2101 agtgcagccg cgggtgcgcg aggcgcttta tctggtagac gtaatccgcc gcaaccagtt
     2161 cgcgcgtgcc cagaggcagg gcgtagggcg tttcgagcgt cttgatctgc tcggcggtca
     2221 atccatggtt cttttccaag aacgctgggt ggggctggta gtgaatgccc gggcggatgc
     2281 ggatttcgta gacggtgaac ttgccgcctt cgatttcctt cggcttcggc agctcggctg
     2341 cggtcagcgc gatcagctcg tagggccgct tcaagtagtg atactgcagc ggcggctcga
     2401 ggatctgacg ggtgaagagc gcctcgtcga cggtgtagga ctgcaccggg tcgaggtgct
     2461 tgggccgctg cacgaacgcc gcgtacagga tgttcgcgcc tcgctccgag cgcgggtaag
     2521 ggtcgttcca gacctcgccg cagcccgcga cgcataccac gagcatcagg aggatgcgca
     2581 cgccgctcag tataatcggc cgcatggcgc ttctcgccgg caagaagatc atggtcacgg
     2641 gggtgctctc caaccgctcg atcgcgtacg gcgtggcgaa ggttgcgcgt cgtgaaggcg
     2701 ccgaaatcgc gctcacttac cagaacgagc gcttcaagga ccgcgtcacc gagatggcga
     2761 aggagctcgg cgccgacatc gcgctcccct gcgacgtgag cgttgatcgt gagatccagg
     2821 atcttttctc gcgcctcgcg gaaaactggc agcgcctcga tggcctggtg cacgcgattg
     2881 cgttcgcgcc gcgcgaggcg atcgcgggtg acttcctcga agggatgagc cgcgaggcgt
     2941 tccgccaggc gcacgacatc tcgtcgtaca gcttcgcggc gctggcgaag ggcgcgatgc
     3001 cgctcctgca ggaaagcaaa gcctcgctcc tcacgctcac ttacctcggc gccgagcgcg
     3061 tggtcccgaa ctacaacacg atgggactag ccaaggcgag cctggaag
//
LOCUS       DASUNT010000528         3014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1034672, whole genome shotgun sequence.
ACCESSION   DASUNT010000528 DASUNT010000000
VERSION     DASUNT010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034672"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFX94_10640"
     CDS             complement(<1..450)
                     /locus_tag="VFX94_10640"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HEX5768682.1"
                     /translation="MYEILVFLFENCQQAELAYDRERVAKKLSAAGFEDSDITEALHW
                     LAGMALTPCRAGKPLPDAHSSFRAYAPRELAKLDAECRGFLLTLEHCGVLSAETRELV
                     LERALAASGNTLNLEQLKLVVLMVLWNRQTPTSRLVAEELFSAPQARL"
     gene            complement(491..1591)
                     /gene="dprA"
                     /locus_tag="VFX94_10645"
     CDS             complement(491..1591)
                     /gene="dprA"
                     /locus_tag="VFX94_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX5768683.1"
                     /translation="MNPDPGLAGWLQLALTPGLGPASFQRLLKQFGLPQAVLARKRAE
                     LASFITPTGLAALDSEAVAQAVARALAWAAQPGCSIVTLADDVYPRLLLEISDPPPLL
                     YAQGRIELLARPTLAIVGSRNATQQGEANAGSFAKTLSDAGLTIVSGLALGIDAAAHR
                     GGIAGASSTVAVLGTGADVIYPSRNAALAAQIAERGLLITEFPLGTAPAAHNFPRRNR
                     LISGLARGCLVVEAALASGSLITARAAADQGREVFAIPGSIHSPLSKGCHALIKSGAK
                     LVESAEDVLSELSGFRPTASASTTQDPPSANDSGLLAHMGHDPVDVDALCSRAGMSAE
                     QVCSELLRLELHGRVAALPGGLYQRLEKGQRR"
     gene            complement(1604..2752)
                     /locus_tag="VFX94_10650"
     CDS             complement(1604..2752)
                     /locus_tag="VFX94_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX5768684.1"
                     /translation="MVQQVGKSIIALAFFVSSSAFTQAPGTSAPLTIKPDAPDRYVVV
                     PGDTLWGISERYTDSPWRWPELWNMNREQIRNPHLIYPGYVILVDRDKGRLTIAPPGT
                     GTDAKPGVPADGATPGVAPDGAPTAASGSVKLSPRVRAESLAKQEIPSIPASAIEPFL
                     SRPLVIEPDGLEKAPTIIATQQDRVVLAAGNAAYVRGMGGSKDDTWYVYRKGTALVDP
                     DTNKTLGYEAIYLGTAQLTRPGEPATVLLTSAVQEVGAGDKLVAAGRPQPVNYAPHAP
                     GTQIKGRVIAIYGGVANVGEAGPQSIISINRGKADGLEIGHVVALYNRGATVRDTSKP
                     RKAPDARIQLPDERAGLAFVFRVFDRISYALIMQVTRPVRPLDVVQTP"
     gene            2883..>3014
                     /locus_tag="VFX94_10655"
     CDS             2883..>3014
                     /locus_tag="VFX94_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX5768685.1"
                     /translation="MARLNILRYPDARLHKVAAPVTVFDETLKRLVADMAESMYAAPG
                     "
BASE COUNT          514 a          959 c          994 g          547 t
ORIGIN      
        1 cagacgagct tgcggggcgg aaaagagctc ctccgccacg agccggctgg tcggcgtttg
       61 ccggttccac agcaccatca gcaccaccag cttgagctgc tcgaggttga gcgtgtttcc
      121 cgacgcggcg agcgcgcgct cgagcacgag ctcacgcgtc tcggcgctaa ggacaccgca
      181 atgctctagc gttaggagaa agccgcggca ctcggcatca agtttcgcca gctcgcgcgg
      241 cgcgtacgcg cggaagctcg agtgcgcgtc gggcaggggc ttgcccgcgc gacacggcgt
      301 gagcgccatc ccggcgagcc agtgcagcgc ctcggtaatg tcggaatcct cgaatccggc
      361 cgccgacagc tttttcgcca cccgttcccg gtcgtaggcg agctcggcct gctggcagtt
      421 ctcgaacagg aaaacgagga tctcgtacat ggtttttccc ttcttattaa cgcgacgatg
      481 gggattgcgt ttacctgcgt tgtcccttct ccagccgctg gtaaagtccg cccggaagcg
      541 cggccacgcg gccgtggagc tccaggcgca gaagctcaga acatacctgc tcggccgaca
      601 tgccagcgcg cgagcagagc gcgtccacgt cgaccggatc gtgccccatg tgagcgagaa
      661 gcccggaatc gttcgccgac gggggatcct gggtggtgga tgcagatgcc gtcgggcgga
      721 atccgcttag ctcactcaac acgtcttcgg ccgattcgac cagcttcgcg cctgacttga
      781 tgagggcgtg gcaacctttc gaaagcggcg agtggatcga gcccggaatc gcgaatacct
      841 cgcgaccttg atctgccgcg gcgcgggcgg tgatcagaga acccgacgcg agcgccgctt
      901 cgacgaccag gcaaccgcgc gcaaggccgc tgatcagccg gttacggcgc ggaaagttgt
      961 gtgccgccgg tgccgtgcca agcgggaatt cggtaatgag aagcccgcgc tcggcgatct
     1021 gcgcggcgag ggcggcgttg cggctcgggt aaatcacgtc cgcgccggtt ccgagcacag
     1081 cgacggtgga tgaggcgccg gcaatgccgc cgcgatgtgc tgcggcatcg atgccgagcg
     1141 cgagcccgga aacgatcgtc agccccgcgt cgctcaatgt ctttgcgaac gatcccgcgt
     1201 tggcctcgcc ctgttgcgtg gcgttgcggc tgcccacaat ggcgagcgtc ggccgcgcga
     1261 gtaattcgat gcggccttgc gcatagagca acggcggcgg atccgaaatt tcgagcagga
     1321 ggcgtggata gacgtcgtct gccagcgtga ctatcgagca gcctggctgc gcggcccacg
     1381 ctagtgcgcg cgcaacggcc tgcgccacgg cttcggagtc gagcgccgca agcccggtcg
     1441 gggtgatgaa cgaagcgagt tcggcgcgct tcctcgcgag aaccgcctgc ggcagcccga
     1501 attgcttgag gagtcgctgg aaacttgccg gaccaaggcc cggggtcagc gccagctgca
     1561 gccagcctgc cagccccggg tcgggattca cgtcgtgctc ttcttacggc gtctgcacca
     1621 catcgagcgg acggaccggc ctcgtgacct gcatgataag cgcataggat atccggtcga
     1681 atacccggaa cacaaacgcc agtcccgcgc gctcgtcggg caattggatg cgcgcgtccg
     1741 gcgccttgcg cggcttgctc gtatcgcgca ccgttgcgcc acgattgtag agcgcgacca
     1801 catggccgat ttcgaggccg tcggcctttc cgcggttgat gctgatgatg gactgcggac
     1861 cggcttcgcc tacgttggct acgccgccat agattgcgat aacgcggccc ttgatctgcg
     1921 taccgggcgc gtgcggagca tagttcaccg gctgaggacg tccggcagcc accagcttgt
     1981 ctccggctcc cacctcctgc acggcactgg tgagcagcac cgtcgccggc tcaccggggc
     2041 gcgtgagctg cgccgtgccg agatagatcg cctcatagcc gagcgtcttg ttggtatcgg
     2101 ggtctaccag cgcggtgccc ttgcgataca cataccaagt gtcgtccttc gagccgccca
     2161 tgccacgcac gtatgcagcg ttgccggcgg cgagcactac tcggtcctgc tgtgttgcga
     2221 tgatggtggg ggccttttca agaccatcgg gctcgatcac tagcgggcgc gacagaaagg
     2281 gttcgattgc cgaagctgga atgctcggga tctcttgctt cgcgagcgac tcggcgcgca
     2341 cgcgcgggct gagcttgacg ctacccgaag ccgcggtcgg cgcgccatcc ggcgctacgc
     2401 ccggagttgc gccgtccgcc ggtacgccgg gcttggcgtc cgtcccggtt cccggaggcg
     2461 ctatcgtcag ccggcccttg tcgcggtcga ccagaatcac gtagcccggg tagataaggt
     2521 gcggattgcg gatctgttcc cggttcatgt tccacagctc cggccagcgc cagggcgagt
     2581 cggtgtagcg ctcagagatg ccccacaggg tgtcacccgg aaccacgacg taccggtcgg
     2641 gcgcatcggg ctttattgtg agcggcgccg acgtgccggg cgcctgcgtg aaagcgctcg
     2701 aggacacgaa gaaagcgagc gctatgatag acttaccgac ttgctggacc atggcgatcc
     2761 gtccctttcg cggttcctgg accggctgga aattcgattt ttgaccccaa attagcacaa
     2821 aggctgggtt tcttatgcgc cgcgtgagat tgtccagata atcctttcaa ttgcaagtga
     2881 aaatggcccg cttgaatata cttcgttacc ccgatgcccg gctgcacaag gtggcggctc
     2941 cagtaaccgt tttcgatgag actctcaagc gactggtcgc cgacatggcg gagtctatgt
     3001 atgcggcccc cggg
//
LOCUS       DASUNT010000529         4687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1299283, whole genome shotgun sequence.
ACCESSION   DASUNT010000529 DASUNT010000000
VERSION     DASUNT010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299283"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VFX94_10660"
     CDS             complement(<1..469)
                     /locus_tag="VFX94_10660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5768686.1"
                     /translation="MREAIAKLCERFPDEYWLKRDREGGFPADFHQAFARDGWLGIAM
                     PEAYGGAGLGITEAAVMMQAIAESGAGFSGASAVHMNIFGLNPVVVFGSQEQKKSWLP
                     PLIAGKERACFAVTEPNTGLNTRQIKVKAEKKDGKYLVSGQKVWISTAQVAEKV"
     gene            complement(499..1254)
                     /locus_tag="VFX94_10665"
     CDS             complement(499..1254)
                     /locus_tag="VFX94_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5768687.1"
                     /translation="MAAGCAATPEPEALKDLAPNGTLRAAINVGNAVLAQRGPAGGEP
                     TGVSVDMARELARRLGVPVHLVVYDAAGKVVEAGRAGAWDIAFVALDPARAAEIAQSP
                     PYVQIEGAYVVRQSSPLRSNDEVDRQGHRVVVARNSAYDLFLSRALKEAEIVRTPTSA
                     TVVDVFMKENYEVAAGVKQQLEADARRVPGLRLLPGRFMVINQAMASQKGREAGARYV
                     REFVEDVKASGFVANALERHKQTAAVAPPGDPK"
     gene            complement(1297..2001)
                     /locus_tag="VFX94_10670"
     CDS             complement(1297..2001)
                     /locus_tag="VFX94_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5768688.1"
                     /translation="MPSRLLEGDSALVTGAARGIGRGIADALEAEGARVFRADVDQAD
                     LSKPGEAASLADEAIRSLGSVSILVHCASPRRQENQTVLQATWEQWREMLSINLDAAF
                     VLTQKIGRHMKDNGIKGRMLIVTSLHAESPRNLPHYSAAKAGQTMLVKELARALGPHG
                     IRVNAIAPGAIPGGGFQAPPGMVEKIPLGRFGTPADVAGMALAVLSERFGAYVTGASI
                     VVDGGIALHNWIEPAE"
     gene            complement(1982..>4687)
                     /locus_tag="VFX94_10675"
     CDS             complement(1982..>4687)
                     /locus_tag="VFX94_10675"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5768689.1"
                     /translation="DSVVSKIEADAQAIIWLGLSSDKHSSLELSDYADRYIADRLKTL
                     PGVASVTIGGGRRYAMRIWLDRERLAAYSLTPQDVENALKRQNVEIPAGRIESARREF
                     TVLAEADLRTAEQFNNMIIVENRGYPVRLRDVGSARLGALDERSVIRRNGQPGVGLGV
                     VKQSTANTLSVAQAAKAEMARIQPTLPEGMQLQISFDSSLFIERSIDSVYRAMGEAVL
                     LVMAVIFVFLRSLRATLIPFVTIPVSLVGSFFFLYLMGFTINVLTLLGVVLAIGLVVD
                     DAIVVLENCHRHVEMGKSPVQASADGSREIAFAVVAMSLTLTAVFAPVAFVPGNTGKL
                     FAEFALTVAAAVAVSGFVALTLTPMMCSKILKEHEKHGGIYLAMERFFHGMTQAYRRA
                     VGFTLRKRFIAVAAFAVVVAGTVFVYQKLRHELSPLEDRGYFLTLINAPEGASMEYTD
                     SYVRAVERMYADVPEIRSLFAVVAPGLERPNPVNFGVTFAQLAPWEERDRRTQQITAS
                     MAPRLSASLPGVLAFPVNPNSLGQGFRTPQVQFVIQDNTYEDLDVSVNKILEKTRASG
                     SIVNADSDLKLNKPQLSVDIDRDKAAALGIDVEVIGRTLETLLGGRQVTRFKREGKQY
                     DVMVQLEAKDRSTPADLTAIYVRARDGTLVQISNLVRVRETVAAKELNHFNRARAAII
                     TANVAPGSTLGEALDFLDETAKQVLPATARTDLDGVSREFRESGAGLAFVILFAALFI
                     YLMLSAQFESFVSPFVIMLTVPLAGLGALAAIWLTGGTLNVYSKVGLVMLIGLITKHG
                     ILIVEFANQLRDRGLPAAEAVAEAATLRLRPILMTTGAMVLGALPLALAAGAGAEARQ
                     AIGWVIVGGLTVGTLFTLFVIPTAYLLLVRSRKAPVKVTSAVPAA"
BASE COUNT          755 a         1570 c         1594 g          768 t
ORIGIN      
        1 gaaccttctc ggcgacctgc gcggtcgaga tccagacttt ctggccggaa acgaggtatt
       61 tcccgtcttt cttctccgcc tttaccttga tctgtcttgt gttcagtccg gtattcggct
      121 cggtgacggc aaagcacgcc cgctccttgc ccgcgatgag cggcggcagc cagcttttct
      181 tttgttcttg acttccaaac accacaacag ggttcaggcc gaagatgttc atatggacgg
      241 cggaagcgcc ggagaagccg gcgcccgact cggcgatcgc ctgcatcatc acggcggcct
      301 ccgttatgcc gagtcctgcg ccgccgtagg cctccggcat cgcgatgccg agccagccgt
      361 cgcgggcgaa cgcctggtgg aagtctgccg ggaagccccc ttcacggtca cgcttgagcc
      421 agtactcgtc ggggaagcgc tcgcagagct tcgcgatcgc ttcgcggatc ctttcctgct
      481 ccggggagag cgagaaatct actttgggtc tcccggcggg gcgaccgcag cggtctgctt
      541 gtgtcgctcg agcgcgttgg cgacgaagcc ggaagccttc acgtcctcga cgaactcgcg
      601 cacgtagcgt gcgccggcct cgcggccctt ctggcttgcc atcgcctggt tgatcaccat
      661 gaagcggccg ggtagcaggc gcagccccgg cacgcggcgg gcgtcggctt cgagctgttg
      721 cttcacgccg gcggcgacct cgtagttctc cttcatgaag acgtcgacca ccgtcgccga
      781 agtcggcgtg cgcacgatct cggcttcttt cagggcgcgc gagagaaaga gatcgtaggc
      841 gctgttgcgc gcgacgacca cgcggtgtcc ctggcggtcg acttcgtcgt tggaacgcag
      901 cggagagctt tgccttacga cgtacgcacc ctcgatctgc acgtaaggcg gcgactgcgc
      961 gatctcggcg gcgcgcgcgg gatcgagggc gacgaaggcg atgtcccacg ccccggcgcg
     1021 ccccgcttcg accaccttgc ccgccgcgtc gtacacgacg aggtgcaccg gcacgccaag
     1081 ccgccgcgca agctcccgcg ccatgtctac cgacacgccg gtcggctcgc cgcccgccgg
     1141 ccctcgctgc gcgaggacgg cgttgccgac gttgatcgcc gcgcgcagcg tgccgttggg
     1201 cgcgagatcc ttgagcgctt caggctcggg agtcgccgcg cagcctgcag ccagaagcgc
     1261 tatccccgga agaaatgacc gcactcagcg ccctccctat tcggctggct cgatccagtt
     1321 gtgcagagcg atgccgccat cgaccacgat cgacgcgccg gtgacgtagg cgccgaagcg
     1381 ctccgagagc accgcgagcg ccatgccggc cacgtcggcg ggcgtgccga agcggccgag
     1441 cggaattttc tcgaccatgc cgggcggcgc ctggaagccg ccgccgggaa tcgcgcccgg
     1501 cgcgatggcg ttcacgcgga tgccgtgcgg cccgagcgcg cgcgcgagct ccttcacgag
     1561 cattgtctga cccgccttgg cggcgctgta gtgcggcagg ttgcgcggcg actcggcgtg
     1621 gagcgacgtc acgatcagca tccggccctt gatcccgttg tccttcatgt gccggccgat
     1681 tttctgcgtc agcacgaaag ccgcatcgag gttgatggag agcatctcgc gccactgctc
     1741 ccacgttgcc tggagaaccg tctggttttc ctgccgccgc gggctcgcgc agtgcacgag
     1801 aatcgacacg cttccgaggg agcgaatcgc ttcatcggcc aaactggcgg cttcgccggg
     1861 cttggagaga tcggcctggt ccacatcggc gcgaaatacg cgcgcgccct cggcctcgag
     1921 cgcatcggcg atgccgcggc cgatgccgcg ggcggcgccg gtcacgagtg cgctgtcgcc
     1981 ctcaagcagc cgggacggca ctggtcacct tgactggcgc tttcctgctc ctgacgagaa
     2041 gcaggtacgc ggtcgggatg acgaacagcg tgaagagcgt gcccacggta agcccgccga
     2101 cgatgaccca gccgatcgcc tggcgcgcct cggcgccggc gccggcggcg agcgcgaggg
     2161 gcagcgcgcc aagcaccatc gcgccggtcg tcatcaatat cggccgcagg cgcagcgtcg
     2221 ctgcttcggc caccgcctcg gccgccggca gcccccggtc gcgcagctgg ttggcgaact
     2281 ccacgatcag gatcccgtgc ttggtgatga ggccgatcag catgaccagt cccaccttgg
     2341 aatagacgtt gagcgtgccg ccggtgagcc agattgcggc gagcgcgccg aggcccgcga
     2401 ggggcacggt cagcatgatg acgaagggcg agacgaagct ctcgaactgc gccgacagca
     2461 tcaaatagat gaagagggcc gcgaacagga tgacgaacgc gagacccgcg ccggactcgc
     2521 ggaactcgcg cgacactccg tcgaggtcgg tgcgggcggt cgcggggaga acctgctttg
     2581 ccgtctcgtc caggaaatcg agcgcctcgc cgagcgtcga cccgggcgcg acgttggccg
     2641 tgatgatggc tgcgcgcgcg cggttgaagt ggttcagctc cttggccgcc accgtctcgc
     2701 gcacgcgcac gaggttcgag atctgcacga gcgtgccgtc gcgcgcgcgg acgtagatgg
     2761 cggtgaggtc cgccggcgtg ctgcggtcct tcgcctcgag ctgcaccatc acgtcgtact
     2821 gcttgccttc gcgcttgaag cgcgtcacct ggcgcccgcc gagcagcgtc tcgagcgtgc
     2881 gcccgatcac ctcgacgtcg atgccgagcg cggccgcctt gtcgcggtcg atgtcgaccg
     2941 agagctgcgg cttgttcagc ttcaggtcgg agtcggcgtt cacgatcgat cccgaggcgc
     3001 gcgtcttctc gaggatcttg ttgaccgaga catcgaggtc ttcgtaagtg ttgtcctgga
     3061 tcacgaactg cacctgcggc gtgcggaagc cctggccgag cgagttcggg ttcacgggga
     3121 aggcgagcac tccgggtagc gacgccgaga gccgcggcgc catcgaggcg gtaatctgct
     3181 gggttctgcg gtcgcgctct tcccacggcg cgagctgcgc gaaggtgacg ccgaagttga
     3241 ccgggttggg gcgctccagg cccggcgcca ccaccgcgaa caggctgcgg atctccggca
     3301 cgtcggcgta catgcgctcc accgcgcgca cgtagctgtc ggtgtactcc atcgatgcgc
     3361 cctcgggcgc gttgatgagc gtgagaaagt agccccggtc ttcgagcggc gagagctcat
     3421 gcctgagctt ctggtagacg aatacggttc ccgccacgac aacggcgaac gccgcaaccg
     3481 cgatgaaacg tttcctcaac gtgaacccta cggcgcggcg atacgcctgc gtcatgccgt
     3541 ggaagaaacg ctccatggcg aggtagatgc cgccgtgctt ctcgtgctcc ttgaggatct
     3601 tggagcacat catcggcgtg agggtgagcg cgacgaagcc cgagacggcg accgccgccg
     3661 ccaccgtaag cgcgaattcc gcgaacagct tgccggtgtt gcccggaacg aaggcgaccg
     3721 gggcgaacac cgcggtcagc gtgagcgaca tcgccaccac cgcgaacgcg atctcgcgcg
     3781 agccgtcggc cgaggcctgc acggggcttt tgcccatctc tacatggcgg tggcagttct
     3841 ccagcaccac gatggcgtcg tcgaccacga ggccgatcgc gagcaccacg ccgagcagcg
     3901 tgagcacgtt gatcgtgaac cccatgaggt agaggaagaa aaacgagccc acgagcgaca
     3961 ccgggatggt gacgaagggg atgagcgtcg cgcgcagcga gcgcaggaaa acgaagatca
     4021 ccgccatcac gagcagcacc gcctcgccca tcgcgcggta aaccgaatcg atcgagcgct
     4081 cgatgaagag cgatgagtcg aaggagatct gcagctgcat gccttcgggc agcgtgggct
     4141 ggatgcgcgc catctccgcc ttggccgcct gcgccacgga gagcgtgttg gcggtggact
     4201 gcttcacgac gccgagcccg acgcccggct gcccgttgcg gcggatgacg ctgcgctcat
     4261 ccagggcgcc gaggcgcgcc gagcccacgt cgcgcagcct caccgggtag ccgcggtttt
     4321 ccacgatgat catgttgttg aactgctcgg cggtgcgcag gtcggcttcg gcgagcacgg
     4381 tgaactcgcg gcgcgccgac tcgatgcgcc ccgccgggat ctcgacgttc tggcgcttga
     4441 gcgcgttctc cacgtcctgc ggcgtgagcg agtaggcggc gagcctttcg cggtccagcc
     4501 agatgcgcat cgcgtagcgc cggccgccgc cgatcgtcac cgaggcgact ccgggcagcg
     4561 tcttcagacg gtcggcgatg tagcggtcgg cgtagtcgga gagctcgagc gacgaatgct
     4621 tgtcgctcga caggcccagc cagatgatgg cctgcgcgtc ggcctcgatc ttggagacga
     4681 ccgagtc
//
LOCUS       DASUNT010000530         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_71009, whole genome shotgun sequence.
ACCESSION   DASUNT010000530 DASUNT010000000
VERSION     DASUNT010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71009"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="VFX94_10680"
     CDS             <1..1249
                     /locus_tag="VFX94_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5768690.1"
                     /translation="REARAGMPFRRAGQGSRTGGLSVQGRLPENVMHFARLLRAAGLR
                     VGSDRVIDCVRALEIAGAHQFPLRRDDWYWTMSAVLLSRQEQRPIFDQAFRIFWRDPK
                     LAERMMSLLLPQAHGRAAKPEEQQSQRLTDALFSQKEEAQETEKLELEARLTFSSREV
                     LSRMDFDTMSAAELAEAKKMLAQLRLPLPLIRTRRKRMSPKGKAIDLRATLRESLREG
                     GEVIALVRTAPRTLHPPLVVLCDISGSMNPYSRMFLHFLHAITNDRDRVSVFVFGTRL
                     TNITRQLRHRDVDVAMTKVADAIKDWSGGTRIGASLREFNYKWGRRVLAQNASVLLVS
                     DGLDREAGEGLAEEMERLAKSCRQLVWLNPLLRYDKFEARPAGVRAMLPHVDLFLPVH
                     NLKSLVDLARTLSAPIKEKPQWR"
     gene            1246..1839
                     /locus_tag="VFX94_10685"
     CDS             1246..1839
                     /locus_tag="VFX94_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEX5768691.1"
                     /translation="MTGEQLVPASQQATWKALNDPEVLKACVPGCEQVTPVTENEYQV
                     QMTARVGPVSAKFRGRLSIFDVKAPNSYSLAFEGQGGAAGFAKGAAQVKLSAEGAQTR
                     LTYAVKANVGGKLAQIGSRLVDAAAKKVADEFFRNFNDKMSAPAEETVPAHAQAGGHG
                     AAPAEDAAMPKISNTALMFFAAGALVVFIVALVAFFG"
     gene            1832..2533
                     /locus_tag="VFX94_10690"
     CDS             1832..2533
                     /locus_tag="VFX94_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004526445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEX5768692.1"
                     /translation="MAELLFRDDAYLAACEATVVSVHENAVELDRTVFYPLGGGQAGD
                     TGRIGGWRVLDTRKSANSVLHLLEPGAKPSPGEKLEVRIDWERRYRLMRLHTALHVLG
                     AVVKAPVTGGRIAEDKAHLDFDIEMDKLVKDEIEARVNELIAAGRATSSYWISDAELD
                     ARPELVRTMSVVPPRGEGRVRLLEIPGIDLQACGGTHVRNISEIGRLAVARIRSEGKR
                     NKRVTVILADHGAKA"
     gene            2517..>2717
                     /locus_tag="VFX94_10695"
     CDS             2517..>2717
                     /locus_tag="VFX94_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768693.1"
                     /translation="MAQKPERRPAAILFTELRNFTRLSEVLQPDKVLELANEFFSLAA
                     RAIKDHTGKVLSVHNDGLVAAFV"
BASE COUNT          475 a          893 c          922 g          427 t
ORIGIN      
        1 tcgagaagct cgcgcgggaa tgccttttcg ccgcgccggc caaggcagca ggaccggcgg
       61 cttgagcgtg caaggccgcc tgcccgaaaa cgtgatgcac ttcgcgcgcc tgctgcgcgc
      121 ggcgggactg cgggtgggct ccgaccgcgt catcgactgc gtgcgcgcgc tggagatcgc
      181 gggcgcgcac cagtttccgc tcaggcgcga cgactggtac tggaccatgt cggcggtgct
      241 gctctcgcgc caggagcagc gcccgatctt cgaccaggcg ttccgcatct tctggcgcga
      301 ccccaagctc gccgagcgca tgatgagcct gctgctgcca caggcgcacg gccgcgcggc
      361 gaagccggag gagcagcaga gccagcgcct caccgatgcg ctgttctccc agaaggagga
      421 agcgcaggaa accgagaagc ttgagctcga ggcgcgcctc actttctcct cgcgcgaggt
      481 gctctcgcgc atggacttcg acaccatgtc ggcggccgag ctcgccgagg cgaagaagat
      541 gctggcgcag ttgcgactgc ctttgcctct gataagaacc cgaagaaaaa ggatgtctcc
      601 gaaaggaaaa gctatcgacc ttcgtgcgac tttgcgggag agcctgcgcg aaggcggcga
      661 ggtgattgcg ctggtgcgca cggcgccgag aacgctgcac ccgccgctcg tcgttctgtg
      721 cgacatctcg ggttcgatga atccgtactc gcgcatgttc ctgcatttcc tgcacgccat
      781 caccaacgac cgcgatcggg tctcggtgtt cgtgttcggc acgcgcctca ccaacatcac
      841 gcgccagctg cgccaccgcg acgtggacgt cgccatgacc aaggtggccg acgccatcaa
      901 ggactggtcg ggcggcacgc gcatcggcgc gagcctgcgc gagttcaatt acaagtgggg
      961 ccggcgggtg ctcgcgcaga atgcgagcgt gctgctcgtt tccgacggcc tcgaccgcga
     1021 ggcgggcgag gggctcgccg aggaaatgga gcggctcgcc aagtcgtgcc gccagctcgt
     1081 gtggctgaac ccgctgctgc gctacgacaa gttcgaagcc cgcccggcag gcgtgcgcgc
     1141 catgctgccg cacgtcgatc ttttcctccc ggtgcataat ctcaagagcc tcgtggacct
     1201 cgcgagaacg ctaagcgctc cgatcaagga gaaaccccag tggagatgac cggcgagcag
     1261 ctcgtccccg cttcacagca ggcgacctgg aaggcattga acgacccgga ggtgctcaag
     1321 gcctgcgtgc ccggctgcga gcaggtcacg ccggtgaccg agaacgagta ccaggtgcag
     1381 atgaccgccc gggtggggcc ggtgagcgcc aagttccgcg gtcgcctttc gatcttcgat
     1441 gtgaaggcgc ccaattccta ctcgctcgcc ttcgagggcc agggcggcgc cgcgggcttc
     1501 gccaaagggg cggcccaggt gaagctttct gcggaaggcg cgcagaccag gctcacgtat
     1561 gcggtgaagg ccaacgtggg cggcaagctc gcgcagatcg gctcgcgact ggtggacgcc
     1621 gcggccaaga aagtggccga cgagttcttc cgcaacttca acgacaagat gagcgcgccg
     1681 gccgaggaaa ccgtgcccgc gcacgcgcag gccggagggc atggcgccgc gccggccgaa
     1741 gatgccgcga tgccgaaaat ctccaacacc gcgctgatgt tcttcgccgc gggcgcgctc
     1801 gtcgtgttca tcgtcgccct cgtcgccttc tttggctgag cttcttttca gggacgacgc
     1861 ctacctcgcc gcctgcgagg cgacggtcgt gtcggtgcat gaaaacgcgg tcgagctcga
     1921 tcgcacggtg ttctacccgc tcggcggcgg ccaggcgggc gacaccgggc gcatcggcgg
     1981 ctggcgggtg ctcgatacga gaaaaagcgc aaattccgtc ctgcacctgc tcgagccggg
     2041 agctaagccc tcccccgggg aaaagctcga ggtccgcatc gactgggagc ggcgctaccg
     2101 gctgatgcgg ctgcacaccg cgctgcacgt tctcggcgcg gtggtaaagg cgccggtaac
     2161 cggcgggcgc atcgccgaag acaaggcgca cctcgacttc gacatcgaga tggacaagct
     2221 ggtgaaggac gaaatcgaag cgcgagtcaa tgaattgatc gcggcgggcc gtgccacgtc
     2281 ttcgtactgg atcagcgacg ccgagctcga tgcgcggccg gagctggtgc ggaccatgtc
     2341 ggtcgtgccg ccgcggggcg aggggcgggt gcgcctcctg gaaatccccg gcatcgacct
     2401 gcaggcctgc ggcggcaccc atgtgagaaa tatttccgag atcggccgcc tcgcggtggc
     2461 gcgcatccgg tccgagggca agcgcaacaa gcgcgttacg gttatcctcg ccgaccatgg
     2521 cgcaaaagcc tgaacgccga cccgccgcca tccttttcac cgagctgcgc aacttcacgc
     2581 ggctgtccga agtgctgcag cccgacaagg tgctcgagct cgcgaacgag ttcttctcgc
     2641 tcgccgcgcg cgccatcaag gaccacaccg gcaaggtgct ctcggtgcac aacgacgggc
     2701 tggtggcggc gttcgtg
//
LOCUS       DASUNT010000531         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1034740, whole genome shotgun sequence.
ACCESSION   DASUNT010000531 DASUNT010000000
VERSION     DASUNT010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034740"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VFX94_10700"
     CDS             complement(<1..539)
                     /locus_tag="VFX94_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5768694.1"
                     /translation="MLRVQDLVTAYGKIEALKGVSLEVAQGAITCLLGPNGAGKTTLM
                     MTIAGILRPRAGSISFLGQALSGLAPHAIVARGIALVPENRLVFPSMTVLENLHAGAY
                     QRNDAFEEDIERLFRRFPRLRERAAQAAGTLSGGEQQMLAVARALRSRPKLLLMDEPS
                     LGLAPLVVEEIFAIIAELHR"
     gene            complement(539..1282)
                     /locus_tag="VFX94_10705"
     CDS             complement(539..1282)
                     /locus_tag="VFX94_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5768695.1"
                     /translation="MLELRALSRHFGGVKAIDKLDLTVRQGEIFGLIGPNGSGKSTTV
                     NVIAGVFPPTAGEIRFEGESLDKLPTHRRVQHGIARTFQNIRLFGQLSVWQNLWVAQN
                     APGERKRRGFLSRWLGGQAGVRAEISELLEFSGLSHKQDELAANLAFGEQRRLELARA
                     LAARPKLLLLDEPAAGMNNEEIGQLRDRIFKLRAQGITIVLIEHVMELVMGVTDRIAV
                     LNFGQKIAEGTPAEIQADALVRKAYLGGV"
     gene            complement(1275..2138)
                     /locus_tag="VFX94_10710"
     CDS             complement(1275..2138)
                     /locus_tag="VFX94_10710"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5768696.1"
                     /translation="MDFYYGLAQTIGIHTLLGLSAYVVLLTGQVTMAQAAFFAVGAYA
                     AGMLTVLGGWPILPALGAGGVLGAATACAVGFPALRVKGLMLVVATLAFGEGVRLFFF
                     NFDYQVAKGDIKVGPLGGEGFRQIRYFQENGWSTGEVMLFIWVFVLAAMGLLWWFDRS
                     RYGAVLRAVGEDELAAQSVGVNLTAVKVSAMSAGGAIAGIGGGLYAHYTTHIEHANFN
                     VLLATFAIAYPIVGGLKNVFGTLAAVIFVQAFLIEGLRFLGDWRNLLFGALIVAAMNL
                     RPYGFLGGRRA"
     gene            complement(2138..>2459)
                     /locus_tag="VFX94_10715"
     CDS             complement(2138..>2459)
                     /locus_tag="VFX94_10715"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5768697.1"
                     /translation="CGIRVGRVNGTTFVITGLLGGIAGALIGASVGALSPLLTAPLTV
                     KGLIVTVIGGLGSIPGAIIAGFLVGAFENVFQQFRGVTERDLYVMLLLFAFLVFRPAG
                     LFKS"
BASE COUNT          451 a          837 c          803 g          368 t
ORIGIN      
        1 cggtgcagct ccgcgatgat ggcgaagatc tcttcgacca ccagcggcgc gagcccgaga
       61 gagggctcgt ccatcagcag cagcttcggc cgcgacctca gcgcacgcgc caccgccagc
      121 atctgctgct cgccgcccga gagagtgccc gcggcctgcg cggcgcgctc tctaaggcgg
      181 ggaaagcggc gaaacaggcg ctcgatgtcc tcttcgaacg catcgttcct ctgataggcg
      241 cccgcgtgca ggttctcgag caccgtcatg ctgggaaaga cgagccggtt ttccggcacg
      301 agcgcgatac cccgggcgac gatggcgtgc ggcgcgaggc ccgaaagtgc ctggccgagg
      361 aagctgatcg aacccgcccg gggcctcagg atgcccgcga tcgtcatcat gagcgtcgtc
      421 ttgcctgcgc cgtttggccc gaggaggcag gtgatcgctc cttgcgccac ttcgagcgac
      481 acgcccttca acgcctcgat cttgccgtac gcggtcacca gatcctggac gcgaagcatc
      541 agacgccgcc gaggtaggcc ttgcgcacga gcgcatcggc ctggatttcg gccggtgtgc
      601 cttcagcgat cttctgtccg aagttgagca ccgcgatacg atcggtgacg cccatcacca
      661 gctccatgac gtgctcgatc agcacgatcg tgatgccttg cgcgcgcagc ttgaaaatcc
      721 ggtcgcgcag ctggccaatc tcctcgttgt tcatccccgc ggccggctcg tccagcagga
      781 gcagcttcgg gcgcgcggcg agagcgcgcg cgagctccag ccggcgttgc tcgccgaacg
      841 caaggttcgc ggcgagctcg tcctgcttgt gcgaaaggcc gctgaactcg agcagctcgc
      901 tgatctcggc gcgcaccccg gcctggccgc cgagccagcg cgagaggaag ccgcggcgct
      961 tgcgctcccc gggcgcgttc tgggcgaccc agaggttctg ccacaccgaa agctggccga
     1021 agaggcggat gttctggaac gtgcgcgcga tgccgtgctg cacgcgcctg tgagtcggaa
     1081 gcttgtccag gctttcgcct tcgaatcgta tttcgccagc ggttggcgga aagacccctg
     1141 cgatgacatt cacggtagtg ctcttgcccg agccgttcgg cccgatcagg ccgaagatct
     1201 cgccttggcg caccgtgaga tcgagcttgt cgatagcctt tacgccgccg aagtggcgcg
     1261 acagggcgcg cagctcaagc acggcgccct ccgaggaaac cgtacgggcg caggttcatc
     1321 gccgccacga tgagcgcgcc gaagagcagg ttgcgccagt cgccgaggaa gcgcagcccc
     1381 tcgatgagga acgcctgcac gaagatcacc gccgcgagcg tgccgaacac gttcttcagt
     1441 ccgccgacga tggggtaggc gatcgcaaac gtggcaagga gcacgttgaa gttcgcgtgc
     1501 tcgatgtgcg tggtgtagtg ggcgtagagg ccgccgccga ttccggcgat cgcgccgcct
     1561 gcgctcatcg cgctcacctt caccgcagtg aggttgacgc cgaccgattg cgccgcgagc
     1621 tcatcctcgc cgacggcgcg cagcaccgcg ccgtagcgcg agcgatcgaa ccaccagagc
     1681 agccccatcg cggccagaac gaaaacccat atgaagagca tcacttcccc ggtgctccag
     1741 ccgttttcct ggaaatagcg gatctggcgg aagccctcgc cgccgagcgg accgaccttg
     1801 atgtcgccct tcgccacctg gtagtcgaaa ttgaagaaga aaagacgcac gccctcgccg
     1861 aaggcgagcg tcgcgacgac cagcatcagc cccttgacgc gcagcgccgg gaacccgact
     1921 gcgcacgccg tggcggcccc gagcactccg cccgcgccca gcgccggcag gattggccat
     1981 ccgccgagga cggtgagcat gccggccgcg taggcgccga cggcgaaaaa ggcggcctgc
     2041 gccatcgtca cctggcccgt gagcagcacg acatacgccg agaggccgag cagggtatga
     2101 ataccgatcg tctgcgccag tccgtagtag aaatccatca gctcttgaag agcccggcgg
     2161 ggcggaagac gaggaacgcg aaaaggagca gcatcacgta cagatcacgc tccgtgacgc
     2221 cgcggaattg ctggaacacg ttctcgaacg cgcccaccag gaagccggcg atgatcgcgc
     2281 ccgggatgct gccgagcccg ccgatcaccg tgacgatcag ccctttcacg gtcagcggcg
     2341 cggtgagaag cggcgaaagg gcacccacgg acgcgccgat cagcgcgccg gcaatgccgc
     2401 caaggaggcc cgtaatgacg aatgtcgtgc cgtttacccg gccgacccgg atgccgcag
//
LOCUS       DASUNT010000532         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_422584, whole genome shotgun sequence.
ACCESSION   DASUNT010000532 DASUNT010000000
VERSION     DASUNT010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422584"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VFX94_10720"
     CDS             <1..948
                     /locus_tag="VFX94_10720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX5768698.1"
                     /translation="VISAIECVHALNSAGKRLPFAIEVIGFGDEEGVRFGTTLLGSRA
                     VAGIFDQKILGAVDSTGKTMSQALTEFGLDPKQIPGIARRKGQVLAYAELHIEQGPVL
                     EAEGLAVGVVTAINGFSRLRVTLRGAAGHAGTVPMKLRRDALAATAECVLTVERIARS
                     HPELVGTVGRLEAKPGAINVIPGEVMFTVDVRAPQDALRKHAVAAIRAETESIARKRN
                     LESAIELLQEFGCTACASGLMEQMARAVERQGFPVRRLPSGAGHDGMALAAIADICML
                     FVRCKGGVSHSPLEAITQADAAVGARVLFDFIEHFRIPA"
     gene            923..1834
                     /gene="puuE"
                     /locus_tag="VFX94_10725"
     CDS             923..1834
                     /gene="puuE"
                     /locus_tag="VFX94_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HEX5768699.1"
                     /translation="MSTSEYPRDLAGYGAKPPRPKWPGGARLALQFVLNYEEGGENSV
                     LHGDKASEAFLSEIVGAQPLEGVRHMSMESIYEYGSRVGVWRLLELFARYEAPLTVFG
                     VAMAMERNPAVVERLLKVGHEIASHGWRWINYQYVPLDVEREHMQRAIEVHRRLTGVR
                     PLGWYTGRTSPNTRRLVAEDGGFVYDADDYNDDLPWYDARYRDKSDKPQLVVPYTLDA
                     NDMRFATAQGFNSGDDFFNYLRDSFDVLYAEGGRMMSVGLHCRLVGRPGRFAALERFL
                     KYVRTKKAWVARRIDIARHWLKHHPHG"
     gene            1827..>2608
                     /locus_tag="VFX94_10730"
     CDS             1827..>2608
                     /locus_tag="VFX94_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5768700.1"
                     /translation="MAEELNPAPRLLMGPGPINADPRVLRAMSAQLLGQFDPQFRAYM
                     KEVSDLYRAVFQTKNRWTLLIDGTARAGIEAALVSLVEPGDKVLVPVFGRFGHLKVEI
                     ARRCGAEVIPMELEWGSVFADEVLEREIKKHKPKVVAVSHGDTSTTMAQPLAELGKLC
                     RQHDALLYVDATATLGGMDLQADAWQLDVVSAGLQKCLGGPSGSAPLTLNERAEKCIV
                     RRRHTEQGLRKAQEPQGEGPVIGSNYFDLAMIMDYWSERALN"
BASE COUNT          458 a          850 c          895 g          405 t
ORIGIN      
        1 gtgatcagcg cgatcgagtg cgtccatgcg ttgaattcag caggtaagag actcccattc
       61 gcaatcgaag tgatcggttt cggtgacgag gaaggcgtgc gcttcggcac caccctgctc
      121 ggcagccgcg cggtggccgg catcttcgac cagaagatcc tcggcgccgt ggattccacc
      181 ggcaagacca tgtcccaggc gctgacggag ttcggcctgg acccaaagca gatccccggt
      241 atcgcgcgca ggaaaggcca ggtcctggcc tacgccgagc ttcatataga gcaaggccct
      301 gtgctcgagg ccgaaggcct cgcggtgggc gtggtgaccg cgatcaacgg cttctcgcgc
      361 ctgcgcgtca cgctgcgcgg cgccgcgggc cacgccggca cggtgccgat gaagctgcgg
      421 cgcgacgcgc tcgccgcgac cgccgagtgc gtgctcacgg tcgagcgcat cgcgcgcagc
      481 caccccgagc tggtcggcac cgtcgggcgg ctcgaggcca agcccggcgc gatcaacgtc
      541 attcccggcg aagtgatgtt caccgtcgac gtgcgcgcgc cccaggatgc gctgagaaag
      601 catgcggtcg cggcgattcg cgccgaaacc gagagcatcg ccagaaagcg aaacctcgag
      661 agcgcaatcg agctgctgca ggagttcggc tgcacggcat gcgcaagcgg cctgatggaa
      721 cagatggcgc gcgccgtcga gcgccagggc ttcccggtgc gccgcctgcc ctcgggggcc
      781 gggcacgacg gcatggcgct cgctgcgatc gccgacatct gcatgctgtt cgtgcgttgc
      841 aagggcggcg tcagccatag cccgctcgaa gcgatcacgc aggccgacgc cgccgtaggc
      901 gcgagagtgc tgttcgattt cattgagcac ttccgaatac ccgcgtgacc tcgccggcta
      961 cggggcgaag ccgccccggc cgaaatggcc cggcggcgcg cgccttgcgc tgcagttcgt
     1021 gctcaactac gaggagggcg gcgagaactc ggttctgcac ggcgacaagg cgtcggaggc
     1081 gttcctctcc gagatcgtgg gcgcgcagcc gctcgaaggc gtgcgccaca tgtcgatgga
     1141 gtcgatctac gagtacggct cgcgcgtcgg cgtgtggcgg ctgctcgagc tcttcgcgcg
     1201 ctacgaggcg ccgctcaccg tgttcggcgt ggccatggcg atggaacgca accccgcggt
     1261 cgtggaacgc ttgctcaagg tcgggcacga gatcgcgagc cacggctggc gctggatcaa
     1321 ctaccagtac gtgccgctcg acgtggagcg cgagcatatg cagcgcgcga tcgaggtcca
     1381 tcgccggctg acgggtgtgc ggccgctcgg ctggtacacc ggccgaacca gcccgaacac
     1441 gcgccgcctg gtggccgagg acggcggctt cgtctacgac gcggacgact acaacgacga
     1501 cttgccgtgg tacgacgcgc gataccgcga caagagcgac aagccgcagc tcgtcgtacc
     1561 gtacacgctg gacgcgaacg acatgcgctt cgccactgca caggggttca actcgggcga
     1621 cgattttttc aactacctca gggacagctt cgatgtgctg tacgccgagg gcggccgcat
     1681 gatgtcggtc gggctgcatt gccggctcgt cggccggccc ggcagattcg ccgcgctcga
     1741 acggtttctg aagtatgtgc gcacaaaaaa agcttgggta gcgcgccgca tcgacatcgc
     1801 gcgccactgg ctgaagcatc accctcatgg ctgaggagct caacccggcg ccgcggctcc
     1861 tcatgggccc agggccgatc aacgccgatc cgcgggtgct gcgcgcgatg agcgctcagc
     1921 tgctcggtca gttcgacccg cagtttcgcg cgtacatgaa ggaggtgtcg gatctctacc
     1981 gcgcggtgtt ccagacgaag aaccgctgga cgctgctcat cgacggcacc gcgcgtgcgg
     2041 ggatcgaggc ggcgctcgtc tcgctcgtcg agccgggcga caaggtgctc gtgccagtgt
     2101 tcggccgctt cggccacctc aaggtcgaga tcgcacggcg ctgcggcgca gaagtgattc
     2161 ccatggaact tgagtggggc agcgttttcg ccgatgaagt cctggaacgc gagatcaaga
     2221 agcacaaacc caaggtcgtc gccgtgtcac acggcgatac ctcgaccacc atggcgcagc
     2281 cgctcgcgga gctgggaaag ctttgcaggc agcatgacgc cctgctctac gtggacgcga
     2341 ccgcaacgct cggcggcatg gacctccagg ccgacgcctg gcagctcgat gtggtaagcg
     2401 ccgggctgca gaaatgcctc ggcggcccat cgggcagcgc gccattgacg ctgaacgagc
     2461 gcgccgagaa gtgcatcgtg cggcgccggc ataccgagca gggactgcgc aaggcgcagg
     2521 agccgcaggg cgagggaccg gtcatcggtt cgaactattt cgatctcgcg atgatcatgg
     2581 actactggtc ggaacgcgcg ctcaacca
//
LOCUS       DASUNT010000533         4144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_247122, whole genome shotgun sequence.
ACCESSION   DASUNT010000533 DASUNT010000000
VERSION     DASUNT010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247122"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFX94_10735"
     CDS             <1..324
                     /locus_tag="VFX94_10735"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5768701.1"
                     /translation="IYDGLGQAAAPENFAALAMRGHWVSYGQASGPLQPLSLAEKSGT
                     FSQPVLFHFTATHLQEMAGNVFRALRDGTIRAEVRHRYPLAAASDAHRDLEARRTSGQ
                     VVLLT"
     gene            complement(317..1288)
                     /locus_tag="VFX94_10740"
     CDS             complement(317..1288)
                     /locus_tag="VFX94_10740"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX5768702.1"
                     /translation="MELAVTLYAHPRLHSRDSEAVRDFLRGKQLDFEVLGRGASRVPL
                     DTHINALYMPDGSYLGYVQYGAAAQTRSGAAAGDYRLQFALEGGCEITLGNRRYTCAG
                     SDAVVTSPGRPHGLRLSADCKRVLAVFSRDALAQQLASLLGAPLRSELVFQPELDTSR
                     GAGRTLVEYLHLALREFNRPDSALHSPLAIREFEQLFMTTLLLAHRHNYSALLHGQPA
                     AVTPRDVKRTVDYIHANLDAPVTLADLVAVAGVPGRTLRQHFRHFKGMAPMAYLRRAR
                     FAEARAELQRRDGRTVTEIARRWGFEHMGRFSAGYRRLFGESPSKTK"
     gene            1428..1703
                     /locus_tag="VFX94_10745"
     CDS             1428..1703
                     /locus_tag="VFX94_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEX5768703.1"
                     /translation="MRKYIVEREIPNIGAADGNAIREVARKSNGIVEGLGGGIEWLES
                     YITGDKVYCVYLARDEEIIRKHAEKGGFPANRIVEVRRMMDPTSALA"
     gene            1700..2596
                     /locus_tag="VFX94_10750"
     CDS             1700..2596
                     /locus_tag="VFX94_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5768704.1"
                     /translation="MKIWKALCMAVLPMVALAQERGLTRATLGGIELEYEIRGDGEPV
                     FLVHAGIFADWFEPLLQEPALTGRYRVISYHRVGYAGSSRVAGPVTIAQQAAHLRLLM
                     RHLRIDRAHVVGHSSGGNIALQLALVAPDVVHSLVIMEPALPVGGSGPQRLLATRPAM
                     VPAVEAFRAGDKARAVDLFMQGVSGPGYRAPLEQKLPHAFDQAVAAADTFFGQELPAV
                     QNWSFTREDAIRIRRPVLAVVGEKSTDVSPIWKQRQEMLLGWLPNAEPFVLPGTTHLL
                     HVENPRAMAEALAEFFARHPLP"
     gene            complement(2600..3535)
                     /locus_tag="VFX94_10755"
     CDS             complement(2600..3535)
                     /locus_tag="VFX94_10755"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5768705.1"
                     /translation="MVFLLLLTGAAWGQEYPARTVRVVVPFPPGGAPDLVGRTLANRL
                     SERLGQPFVVENRTGAGGNIAAEAVAKSAPDGYTLLATSDGPLVINPSVYSAMPFDTL
                     RDFAPISIAASAGLVLMACPSLPASSLQDVLNLSKTRKLTYASSGYGSSQHVAAELLK
                     DSAAIDLTHVPYKGFGAAVGDAMSCNVDLIFGAISTGIPHVRAGKLRPIAVTQPKRHS
                     GLPDTPTFGEAGHPGVIMEAYMGLLAPAGTPRPIVDKLYTEVTAILLEKETAERLTGA
                     GLDLVRSTPEQYAARIRADLAKYARLSKALAAKAE"
     gene            complement(3537..4040)
                     /locus_tag="VFX94_10760"
     CDS             complement(3537..4040)
                     /locus_tag="VFX94_10760"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5768706.1"
                     /translation="MNTGPLFRLPIVAPAGHPLSATALGTAEGAFEHVRDVFAKRVGT
                     YTGAKVSDFQAVQVKLARARCLIDSARYLMRESALVFQGNRLLDIETKLRLRSQNAFA
                     VAQAREAVETLWSFYGANAIYTRDPLQRYLRDVQAASQHFSFNFDIAGSAYGTVAMGG
                     KYANPTM"
BASE COUNT          681 a         1349 c         1472 g          642 t
ORIGIN      
        1 atttacgacg gcctcgggca ggcggcggcg ccggagaact tcgcggcgct cgcgatgcgc
       61 ggccactggg tgagctacgg ccaggcgagc ggcccgctgc aaccgctgag cctggccgag
      121 aagtccggga ccttctcgca gccggtcctc ttccacttca ctgccacgca ccttcaggag
      181 atggccggca acgtattcag ggcactgcgc gatgggacga tccgggcgga ggtgcggcac
      241 cgttatccgc tcgcggcggc gtccgacgcg catcgcgacc tcgaagcacg ccgcaccagc
      301 ggccaggtcg tactgcttac ttagtcttcg aaggcgattc gccgaagagc cggcggtagc
      361 cggcggaaaa gcgccccatg tgctcgaagc cccagcggcg cgcgatctcg gtgacggtgc
      421 ggccgtcgcg gcgctggagc tctgcgcgcg cctcggcgaa gcgggcgcgg cgcaggtacg
      481 ccatcggcgc cattcccttg aagtggcgga agtgctgccg cagcgtgcgg cccggtacgc
      541 ccgcgacagc gaccaggtca gcgagcgtaa cgggagcatc caggttggcg tggatgtagt
      601 cgacagtgcg ctttacgtcg cgaggcgtaa cggctgccgg ctggccgtgg agcagggcgc
      661 tgtagttgtg tcggtgcgcg aggagcaggg tggtcatgaa aagctgctcg aactcgcgaa
      721 tggcaagcgg cgagtgcagc gccgagtccg ggcgattgaa ttcgcgcagc gcgagatgca
      781 ggtactcgac cagcgtgcgg ccggcgccgc ggctcgtgtc gagctcgggc tggaacacga
      841 gctcggaacg cagcggcgcg ccgagcaggc tcgcgagctg ctgcgcgagc gcgtcgcggc
      901 tgaagacggc gagaacgcgc ttgcagtccg cgctcaggcg caggccgtgc ggcctgccgg
      961 gcgaggtgac gaccgcgtcg cttccggcgc aggtatagcg ccgattgccg agggtaatct
     1021 cgcagccgcc ctcgagcgcg aactggaggc gatagtcccc ggccgcggct ccggagcgtg
     1081 tctgcgcggc cgcgccgtac tggacatagc cgaggtagct gccgtcgggc atgtagagcg
     1141 cgttgatgtg cgtgtcgagc ggcacgcgcg acgcgccgcg gcccaggacc tcgaagtcga
     1201 gctgcttgcc gcgcagaaaa tcgcgcaccg cctcgctgtc acggctgtgc agccgcggat
     1261 gcgcatacaa ggtgacggcg agctccatca cggcagggta ctcccggcgg ccgccgccgt
     1321 gcagtcctta cagacataac tctttgccga ttgcggatag gcggcgccaa gcccggattg
     1381 ccgcaccggc gcccgcggcg cgacgatgcg ccgcgaaagg aggcgtcatg cgcaagtaca
     1441 tcgtcgagcg ggagataccg aacatcgggg cggccgacgg caacgcaata cgcgaggtgg
     1501 cgcgcaagtc caacgggatc gtcgaaggcc tcggcggcgg gatcgagtgg ctggagtcct
     1561 acatcaccgg cgacaaggtg tactgcgtgt accttgcgcg cgacgaagaa atcatccgca
     1621 agcacgccga gaagggcggc ttccccgcca accgcatcgt cgaggtgagg cggatgatgg
     1681 atccgacgag cgccctggca tgaaaatctg gaaagccttg tgcatggccg tcttgccgat
     1741 ggtggcgctt gcccaggaga gaggcctgac ccgggcgacg ctcggcggca tcgagctgga
     1801 gtatgaaata cgaggagacg gcgagccggt ctttctggtg cacgccggca tctttgccga
     1861 ctggttcgag cccctgctgc aggagccggc cttgacgggt cgctaccgtg tcatcagcta
     1921 tcaccgggtc ggctatgcgg gcagcagccg cgtggcgggc ccggtcacca tcgcgcaaca
     1981 ggcggcgcac ctgcgcctgc tcatgcgcca cctgcgcatc gatcgagcgc atgtcgtcgg
     2041 ccactcctcc ggcggcaaca tcgcgctcca gctggcgctc gtcgcgcccg acgtggtgca
     2101 ctcgctcgtc atcatggagc cggcgctgcc ggtcggcggc agcgggcccc agcggttgct
     2161 cgcaacccgg ccggctatgg tgcccgcggt agaggcattc cgtgccggcg acaaggcgcg
     2221 cgccgtggac ctcttcatgc agggcgtttc cgggcccggc taccgtgcgc cgctggagca
     2281 gaagcttccc cacgcattcg accaggcggt tgccgccgcc gatacttttt tcggccagga
     2341 gctgcctgcg gtgcagaatt ggtcgttcac acgggaagat gcgatccgca tccgccggcc
     2401 ggtgcttgcg gtcgtcggcg agaagagcac cgacgtatcg ccgatctgga agcaacgcca
     2461 ggagatgctg ctcggctggc tgccaaacgc ggagcctttc gtgctccccg gtacgacgca
     2521 tttacttcac gtcgagaacc cgcgcgcaat ggcggaggcc ttggccgagt tctttgcccg
     2581 ccacccgctg ccctgaaatt cactccgcct tggcggcgag ggccttggag agcctggcgt
     2641 acttggcgag gtcggcgcgg atgcgggcgg cgtactgctc gggcgtgctg cgcaccaggt
     2701 ccaggccggc gcccgtgagg cgctcggcgg tttccttctc gagcagaatg gcggtgactt
     2761 ccgtatagag cttgtccacg atggggcgcg gcgtgccggc aggagcgagg aggcccatat
     2821 acgcctccat gatcaccccg ggatggcccg cctcgccgaa ggtcggcgtg tcgggcaggc
     2881 cggagtggcg cttcggctgc gtgacggcga tcggcctgag cttgccggcg cgcacgtgcg
     2941 gaatcccggt cgagatcgcg ccgaagatga ggtcgacatt gcagctcatc gcatcgccca
     3001 ccgcggcgcc gaagcccttg tagggcacat gcgtcaggtc gatcgcagcc gaatccttga
     3061 gaagctccgc agcgacgtgc tggctgctgc cgtagccgga tgaggcatag gtcagcttgc
     3121 gcgttttact gagattcaag acgtcctgaa gtgaagaagc gggcagcgaa gggcaggcca
     3181 tcagcacgag cccagcgctc gccgcgatgg agatcggcgc gaaatcgcgc agcgtgtcga
     3241 aaggcattgc ggagtacacg ctcgggttga tcacgagcgg gccgtccgaa gtcgcgagca
     3301 gcgtgtagcc gtcgggcgcc gacttcgcca ccgcctccgc ggcgatgttg ccgccggcgc
     3361 cggtgcggtt ctcgaccacg aaaggctggc cgaggcgctc gctcagccgg ttggcgagcg
     3421 tgcggccgac gaggtcgggc gcgccgccgg gcgggaatgg gaccacgacg cgcaccgtgc
     3481 gcgccgggta ctcctgcccc cacgccgccc cggtgagcag aagcaggaag accaccttac
     3541 atagtcgggt tcgcatattt tccgcccatg gcgacggtgc cgtacgccga gcctgcgatg
     3601 tcgaagttga aggagaaatg ctggctcgct gcctgcacgt ctcgcaggta gcgctgcaac
     3661 gggtcgcggg tatagatggc attcgcgccg tagaacgacc agagcgtctc cactgcttcg
     3721 cgcgcttgcg ccacggcgaa agcgttctgt gagcgcagcc gcagctttgt ttcgatatcc
     3781 aacagccgat tgccttgaaa aaccagcgcc gattcgcgca tcaggtagcg cgcggaatcg
     3841 atcaggcagc gagcgcgcgc gagcttcacc tgcaccgcct ggaaatccga gaccttggcc
     3901 ccggtgtagg ttccaacccg cttcgcaaag acgtctctca cgtgctcgaa agcgccttcg
     3961 gcggtgccga gcgcggtggc cgagagggga tgtcccgcgg gcgccacgat cggaagccgg
     4021 aacagcggcc cggtgttcaa cgccgcgcca ggatgttcgc cgcccccccg gcaacgtgta
     4081 agcgcgagcg cgcgccgctc ggtcggatgc cacccagcat ggccgcgagt ctagccgcgc
     4141 acgc
//
LOCUS       DASUNT010000534         5285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_247188, whole genome shotgun sequence.
ACCESSION   DASUNT010000534 DASUNT010000000
VERSION     DASUNT010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247188"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="VFX94_10765"
     CDS             <1..1079
                     /locus_tag="VFX94_10765"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5768707.1"
                     /translation="DLPLMRWLEERMWPTEEHHLSERMVRDGTSLACAEMLRGGITCF
                     NDMYFFPEAALEAALEAGMRVALGLIVVDFATAYASDPDDYLRKGLALRDASRETPLV
                     SFCLAPHAPYTVADATLRHVATLAAELDLPVHGHLHETESEIEHSIREHGVRPLERLR
                     RVGLLGPGLIAVHGVHLLPEEIRLLALHGCSVVHCPSSNLKLASGFAAVHSMLEQGIN
                     VALGTDGAASNNRLDVLTEMRTAALLAKALARDAQALPAHAALRAATLGGARALGLGA
                     RIGSIEPGKRADLAALALRAPELAPCYDPVSLIVYAAGREHVTHVWVDGNLRLRDGVL
                     ENPAAGLDTRWQIWQNSFKSPPGS"
     gene            1111..1680
                     /locus_tag="VFX94_10770"
     CDS             1111..1680
                     /locus_tag="VFX94_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5768708.1"
                     /translation="MKKVWLILIALAFAAGCATEPEKPAPQPAPPPPPKPAPPPEPKP
                     APPPPVEKPKPEPEKPKPVAEKVTFAADVLFDFDKAVIKPEGKSKLDDISNKVRGINL
                     EVVIAIGHADRIGPDQYNQRLSVRRAEAIKAYLVSKGIEGNRVYTEGKGEKQPVTGAK
                     CKGVGGAKLIQCLQPDRRVEIEVIGTRKN"
     gene            1741..2424
                     /gene="ubiG"
                     /locus_tag="VFX94_10775"
     CDS             1741..2424
                     /gene="ubiG"
                     /locus_tag="VFX94_10775"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX5768709.1"
                     /translation="MNVDPAELEKFNALAHRWWDPEGDFRPLHDINPLRLEWIAGHAE
                     LAGATVLDVGCGGGILAEAMARRGARVTGIDLSDKALRVAQLHSHESKLDIRYEKSPV
                     EEFTGEFDLVTCMELLEHVPRPAGMVAACARLVRPGGRVFFSTINRNPKSYLHAVIGA
                     EYVLGLLPKGTHDYTRFIKPSELARWSREAGLRLEETTGMTYNPITRRYRLAADCDVN
                     YLVCCSRDA"
     gene            2417..3064
                     /locus_tag="VFX94_10780"
     CDS             2417..3064
                     /locus_tag="VFX94_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX5768710.1"
                     /translation="MPSRAVLFDFDGTLADTAPDLAAAVNRMRVEQGYEAMPLERLRP
                     FASAGARGLLQAAFRVKPDDAEYKAMRQEFLDLYAESCCGETRLFPGIAELLRELAQR
                     HIRWGVVTNKATRFTERILAHLELKPDCVACGDTTPHLKPHPAPLLHAAGQMHLAPSS
                     CWFLGDDLRDMKAARAAGMRPVAVEWGYHHPESGGPNTWEAEAVIAQPMDLVRLL"
     gene            3061..4011
                     /locus_tag="VFX94_10785"
     CDS             3061..4011
                     /locus_tag="VFX94_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX5768711.1"
                     /translation="MKAVDHNFADIELPKPSPSSRDLLVKVEAVSVNPVDYKQRKQAT
                     GSRILGWDAAGTVAAVGSETELFREGDAVFYAGDVTRPGCNSEFHLVDERIVGRKPKT
                     LDFAQAAAMPLTSITAWEAFFDRMKVQPGKRILIISGAGGVGSIGIQLAKQAGLHVVA
                     TASRAESVAWVRALGADEVVNHRQSLTAQIKTPVDYIANFSGDLDAYWDAMAELVAPQ
                     GALVAIVGNTKPLAMDAVRMKSATVCWELMFTRPRFKTPDMIEQHRLLNKVAEWLDAG
                     KLRCTLKETLSPISGANLRKAHEKLESATMIGKLVLKGWS"
     gene            4099..4605
                     /locus_tag="VFX94_10790"
     CDS             4099..4605
                     /locus_tag="VFX94_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX5768712.1"
                     /translation="MAIAPRLQEFLSARHSEYEVLPHPRTAGSAATAQAAHVPGRRFA
                     KAVVVEDDAGYMLAVLPSTHHVALTQLGAALERRPLRLAKESELAELFADCELGALPA
                     VGAAYQLPTVIEEELDAQPDIYFEAGDHEHMVHMTHAEFMRATGQARRAHFSRIDAGL
                     SRVAGGDC"
     gene            4693..5066
                     /gene="ssrA"
                     /locus_tag="VFX94_10795"
     tmRNA           4693..5066
                     /gene="ssrA"
                     /locus_tag="VFX94_10795"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5160..>5285)
                     /locus_tag="VFX94_10800"
     CDS             complement(5160..>5285)
                     /locus_tag="VFX94_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5768713.1"
                     /translation="EAMAKQGNTIRITSTEQAQAAFRTELAKYAALVKKVGLEPQ"
BASE COUNT          933 a         1836 c         1669 g          847 t
ORIGIN      
        1 acgacctgcc gctcatgcgc tggctcgaag agcgcatgtg gccaacggag gagcatcacc
       61 tttcggagcg catggtgcgc gacggcacgt cgctcgcgtg cgccgagatg ctgcgcggcg
      121 gcatcacctg cttcaacgat atgtatttct ttcccgaggc tgcgctcgaa gctgcgctcg
      181 aggccgggat gcgcgttgcg ctcgggctca tcgtggtgga cttcgccacg gcctacgcat
      241 ccgatccgga cgactacctt cgcaaggggc ttgcgctgcg cgacgcaagc cgcgaaacgc
      301 cgctcgtctc tttctgcctc gccccgcacg cgccctatac cgtcgccgac gcaacgttgc
      361 gccatgtcgc gacgcttgcc gccgagctcg accttccggt tcatggccac ctgcacgaga
      421 cggaaagcga aatcgagcac tcgatccggg agcacggcgt gaggccgctg gaacgactgc
      481 gccgcgtggg gcttctcggg cccgggctga tcgccgttca cggggttcac cttcttcctg
      541 aagaaatccg gctcctagcc ctccatggct gctcggtcgt gcattgccca tcgtcgaatc
      601 tcaagctggc aagcggtttc gctgcggtcc attcgatgct cgagcaaggc atcaatgtcg
      661 cgctcggcac cgacggcgcg gcgagcaaca accggctcga tgtgctcacc gagatgcgga
      721 cggcggcgct gctcgccaag gcgctcgcgc gcgatgctca ggccctgccc gcgcatgctg
      781 cgctccgcgc cgccacgctc ggcggcgcgc gcgccctcgg cctcggcgcg cgcatcggct
      841 ccatcgagcc aggcaagcgc gccgacctcg ctgcgctcgc gttgcgcgct ccggagctcg
      901 cgccctgcta cgaccctgtt tcgcttatcg tgtatgcggc cggtcgcgag cacgtgaccc
      961 atgtatgggt cgacggaaat ctgcggctgc gcgacggcgt gctcgagaat ccggccgccg
     1021 gcttggacac ccggtggcag atctggcaga attccttcaa gagtccccca ggctcttgac
     1081 agcgaataat tttcgctcag cggaggaaaa atgaagaaag tctggctcat cctcatcgcg
     1141 ctcgctttcg ccgccggttg cgcgaccgaa ccggaaaagc ccgcgcccca gcccgcgccg
     1201 ccgcctccgc cgaagccagc cccgccgccc gagcccaagc ccgcgccgcc gccgcctgtg
     1261 gagaagccga agcccgagcc cgagaagccg aagccggtcg ccgaaaaggt cacgttcgca
     1321 gccgatgtgc tgttcgactt cgacaaggcg gtgatcaagc ccgaaggcaa atccaagctc
     1381 gacgacatct cgaacaaggt gcgcggcatc aacctcgagg tggtgatcgc gatcgggcac
     1441 gccgaccgca tcggccccga ccagtacaac cagcggctat cggtgcgccg cgcggaggcg
     1501 atcaaggcct acctcgtctc gaaaggcatc gaaggcaacc gcgtctacac ggaaggcaag
     1561 ggcgagaagc agccggtcac gggcgcgaag tgcaagggcg tgggcggcgc gaagctcatc
     1621 cagtgcctgc agcccgaccg ccgcgtcgag atcgaagtga tcggcacgcg gaagaactag
     1681 aaaaggcatt gcgtcaacat cagaccccgc tccggcgggg ttttttgttt taatagccgc
     1741 gtgaacgtcg atccggccga actggagaaa ttcaacgccc tcgcccaccg ctggtgggat
     1801 cccgaagggg acttccgccc gctgcacgac atcaatccgc ttcgcctgga atggattgcc
     1861 ggccacgcgg agctcgccgg cgccacggtg ctcgacgtgg gatgcggcgg cgggattctg
     1921 gccgaggcaa tggcgcggcg cggcgcgcgg gtcacgggaa tcgacctttc agacaaggcg
     1981 ctgcgcgtcg cgcagcttca ctcgcacgag agcaagctcg acatccgcta cgagaaatcc
     2041 ccggtggaag agttcacggg cgagttcgac ctcgtgacct gcatggagct gctcgagcac
     2101 gtgccgcgac ccgccggcat ggtcgccgcg tgcgcccgcc tcgtgcgtcc gggcggccgg
     2161 gtgttcttct ccaccatcaa ccgcaacccg aagtcgtacc tccacgccgt gatcggcgcc
     2221 gagtacgtgc tcggccttct gccgaaggga acccacgact acacgcgctt catcaagccc
     2281 tccgagcttg cgcgctggag ccgcgaggcg gggcttcgcc tcgaggagac gaccggcatg
     2341 acttacaacc cgatcacgcg ccgctaccgg ctggcagccg actgcgacgt gaactatctc
     2401 gtctgctgct cgcgcgatgc ctagccgcgc cgtcctcttc gatttcgacg gcacgctcgc
     2461 cgacacggcg cccgacctcg ccgctgcggt gaaccgcatg cgcgtggagc agggctacga
     2521 ggcgatgccg ctcgagcgcc tgcggccgtt cgcttcggcg ggagcacgcg gcctgctgca
     2581 ggcggcgttt cgcgtcaagc ccgacgacgc cgagtacaag gcgatgcgcc aggagttcct
     2641 cgatctctat gccgagagct gttgcggcga aaccaggctc ttcccgggca tcgccgagct
     2701 cctgcgcgag ctcgcgcaac gccacatccg ctggggcgtg gtaaccaata aagccacgcg
     2761 cttcaccgag cgcatcctcg cgcacctcga gctcaagccc gactgcgtcg cgtgcggcga
     2821 caccacgccg cacttgaagc cccaccccgc tccgctcctg cacgccgccg ggcaaatgca
     2881 tctcgctcct tcgagctgct ggttcctcgg cgacgacctg cgcgacatga aagccgcgcg
     2941 tgccgccggt atgcggcctg tcgcggtaga gtggggatac caccatcccg agagcggcgg
     3001 cccgaacacc tgggaggcgg aagccgtgat tgctcagccc atggatctcg tgcgcctcct
     3061 atgaaagccg tcgaccacaa cttcgccgac atcgagctgc cgaagccgtc gccttccagc
     3121 cgcgacctcc tggtgaaggt cgaagccgtc tccgtcaacc cggtcgacta caagcagcgc
     3181 aagcaggcaa ccggctcgcg catcctgggc tgggatgccg ccggcacggt cgccgccgtt
     3241 ggttctgaaa cagaattgtt cagagaaggc gatgcggtct tttacgcggg cgacgtaacg
     3301 cgtccgggct gcaacagcga gttccacctc gtcgacgagc gcatcgtcgg ccgcaagccc
     3361 aagacgctcg acttcgcgca agctgccgcg atgccgctga cgtcgatcac cgcatgggaa
     3421 gcgttcttcg accgcatgaa agtgcagccc ggcaagcgca tccttatcat cagcggcgcg
     3481 ggcggcgtcg gctcaatcgg catccagctc gccaagcagg ccggcctgca cgtcgtcgcc
     3541 accgcttcgc gcgccgaatc tgtcgcctgg gtaagagcgc tcggcgccga cgaggtcgtg
     3601 aaccacaggc aaagcctcac ggcgcagatc aaaaccccgg tcgactacat cgccaacttc
     3661 agcggcgatc tggatgccta ctgggatgcg atggccgagc tcgtcgcgcc gcagggcgcc
     3721 ctcgtcgcca tcgtgggcaa caccaagccg ctcgcgatgg atgcggtgcg catgaaaagc
     3781 gcgaccgtgt gctgggagct catgttcacc cggccgcgct tcaagacgcc ggacatgatc
     3841 gagcagcacc ggctgctgaa caaagtggcg gagtggctcg acgccggcaa gctgcgctgc
     3901 acgctgaagg agacgctctc cccgatcagc ggcgcgaacc tcaggaaggc acacgagaag
     3961 ctcgagtccg caaccatgat cgggaagctc gtcctcaagg gctggagctg acgccaagct
     4021 gcccgcagac gccgtccttg cgctgaatca atatccccct ccgccatcgg cgtaaagatg
     4081 gcgcgaaacg gaggatttat ggcgatcgca cccaggctcc aggaatttct cagcgcacgt
     4141 catagcgaat acgaggtttt gcctcatccg cgtaccgcag gcagcgcggc aaccgcacaa
     4201 gccgcccacg tgcccggccg gcgcttcgcg aaggcagtgg tcgtcgagga cgacgccggc
     4261 tacatgctcg cggtgctgcc ctcgacgcac cacgtggccc tgacgcagct cggcgcggcg
     4321 ctggagcgcc ggccgctgcg cctggcgaag gaaagcgagc tcgccgagct tttcgccgat
     4381 tgcgagctcg gcgcactgcc ggccgtcggc gccgcctacc agttgccgac cgtcatcgag
     4441 gaagagctcg acgcccagcc ggatatctat ttcgaggccg gcgaccacga gcacatggtg
     4501 cacatgaccc acgcggaatt catgcgcgct actgggcaag cgcgccgcgc gcatttcagc
     4561 cgcatcgatg ccgggcttag ccgcgtcgcc gggggcgatt gctgaggcgg cggccgagcg
     4621 gcaattgaac ttgcggcatc cggccccatc tatcgaatcg gagcggtaaa atgctccggc
     4681 tacaccgatt ccgggggcga cctggtttcg acgtgggcaa cgaagcagcg cagggcatgc
     4741 cgaggaccag atcacctcgt taatccagtc tggaaacact acaaacgcca acgacgagcg
     4801 tttcgctctc gcggcctaac acccgcggga cgctgcacta gcttgctgat gggctaggcc
     4861 cccgcgtaag cgggggtgca gcgtcattca catcagcctc tcctccgggt ttggccgctt
     4921 ggcccggggg atccgtcgaa gcggctagcc gagcgtcagc ctgccggtgg gcgggcgtaa
     4981 ggcgagatac aaatcaccag gctaagcatg tagtcctgac tgtgtagggc ttacggacgc
     5041 gggttcgatt cccgccgcct ccaccaacac gacggctagc tcccgagccg gcttgttgcg
     5101 ccccgttcgc cgaaagacgt aacggccgtt cttgaccggt acccaccgaa ccgcagtcgt
     5161 tactgcggct ccagccccac ctttttcacc agagcggcgt acttcgccaa ctcggtgcgg
     5221 aacgctgcct gcgcctgctc cgtgcttgtg atgcggatcg tgttgccctg cttcgccatc
     5281 gcttc
//
LOCUS       DASUNT010000535         2565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1123095, whole genome shotgun sequence.
ACCESSION   DASUNT010000535 DASUNT010000000
VERSION     DASUNT010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123095"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..446
                     /gene="rpsK"
                     /locus_tag="VFX94_10805"
     CDS             21..446
                     /gene="rpsK"
                     /locus_tag="VFX94_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX5768714.1"
                     /translation="MASRNAPTRQELQQTASARARKKVKKNVAEGIAHIHASFNNTIV
                     TITDRQGNTLAWATSGHAGFKGSRKSTPFAAQVAAERCGRAAQDCGVKNIEVRIKGPG
                     PGRESAVRALNAIGLKIGSIADVTPIPHNGCRPPKRRRV"
     gene            450..1076
                     /gene="rpsD"
                     /locus_tag="VFX94_10810"
     CDS             450..1076
                     /gene="rpsD"
                     /locus_tag="VFX94_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX5768715.1"
                     /translation="MARNLDKCRHCRREGEKLFLKGEKCFTDKCPVERRAYAPGQHGQ
                     KSGARLSDYGKQLREKQKVRHIYGLLERQFRKTYAEAARSKGVTGERLLQLLESRLDT
                     VVYRMGFGASRTEARQIVRHNGILVNGKRVNIPSYQVRAGDVIEVAGGAKEQLRIKAA
                     AEAAASRGIPEWMEVDSKGLKGTFKNLPARSDLPQTINESLIIELYSK"
     gene            1089..2078
                     /gene="rpoA"
                     /locus_tag="VFX94_10815"
     CDS             1089..2078
                     /gene="rpoA"
                     /locus_tag="VFX94_10815"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX5768716.1"
                     /translation="MPQSGFLKPRIVDVQNLSPNHARVTMEPFERGYGHTLGNALRRV
                     LLSSMPGHAPTEVQIAGVVHEYSTLDGVREDVVDILLNLKGVVFRLHNRAEAILSLKK
                     RGGEGNGAVTAADIEPAHDVEIVNPDHVIAHLAAGGKLEMQIKVESGRGYVPGNMRYL
                     PEETKGIGRIILDASFSPVRRVSYSVESARVEQRTDLDKLIMDIETNGAIEPEEAIRY
                     SARVLVDQLSVFAQLEGTALPTEQPKSPAVDPILLRPVDDLELTVRSANCLKAENIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASRGLTLGMKLENWPPAGLEHRA"
     gene            2082..2528
                     /gene="rplQ"
                     /locus_tag="VFX94_10820"
     CDS             2082..2528
                     /gene="rplQ"
                     /locus_tag="VFX94_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX5768717.1"
                     /translation="MRHAHGLRKLNRTSSHRLAMLRNMTVSLLRHEEIRTTLPKAKEL
                     RRVVEPMITLGKKPSLANRRLAFDRLRDRGIVEKLFDDLGPRYAKRNGGYLRILKIGF
                     RNGDNAPMALVTLMDRPEESAEPKPEEKKKKGEQKTTDETKAAKAA"
BASE COUNT          524 a          865 c          814 g          362 t
ORIGIN      
        1 aagcagggaa ggccgcctaa atggcaagca gaaacgcccc gacgcgccag gagctgcagc
       61 agaccgcctc cgcgcgcgcg cgcaagaagg tcaagaagaa cgtcgccgag ggcatcgccc
      121 acatccacgc gtcgttcaac aacaccatcg tcaccatcac cgaccgccag ggcaacacgc
      181 tcgcgtgggc gacctccggc cacgccggct tcaagggctc gcgcaagtcg acgccgttcg
      241 cggcgcaggt cgcggccgag cgctgcgggc gcgcggcgca ggactgcggc gtgaagaaca
      301 tcgaagtgcg catcaagggg ccgggccccg gccgcgagtc cgcggtgcgc gcgctgaacg
      361 cgatcggtct caagatcggc agcatcgccg acgtaacccc cattccccac aacggttgcc
      421 ggccgcccaa gcggcgccgc gtctagacga tggccagaaa cctcgataag tgccggcatt
      481 gccgccgaga aggcgaaaag ctgttcctga agggcgaaaa gtgctttacc gacaaatgcc
      541 cggtcgagcg gcgtgcgtac gcgcctggcc agcacggcca gaagagcggt gcgcggcttt
      601 ccgactacgg caagcagctg cgcgagaagc agaaggtgcg ccacatctac ggcctgctcg
      661 agcgccagtt ccgcaagacc tacgccgagg cggcgcgctc caagggcgtg accggcgagc
      721 ggctgctgca gcttctcgag tcgcgcctcg acacggtggt gtaccggatg ggcttcggcg
      781 cctcgcgcac cgaggcccgc cagatcgtgc gccataacgg catcctggtg aacggcaagc
      841 gggtcaatat cccgtcgtac caggtgcgcg ccggcgacgt gatcgaggtc gctggaggcg
      901 caaaagagca gctgcgcatc aaggccgccg ccgaagccgc agcctcgcgc ggcattccgg
      961 aatggatgga agtggactcc aaggggctca agggcacgtt caagaacctg cccgcgcgca
     1021 gcgatctgcc ccagacgatt aacgaaagcc tcatcatcga gttgtactcg aagtagagaa
     1081 ggacttacat gccgcaatcg ggattcttaa agccgcgtat cgttgacgtt cagaatctgt
     1141 cgcccaacca cgcacgcgtg acgatggagc ctttcgagcg tgggtacggc cacacccttg
     1201 gcaacgcttt gcgccgcgtg ctgctctcgt cgatgccggg ccacgcgccg accgaagtgc
     1261 agatcgcggg tgtggtgcac gagtactcga cgctcgacgg cgtgcgcgag gatgtggtgg
     1321 acatcctgct caacctcaag ggcgtggtgt tccggctgca caaccgcgcc gaggcgatcc
     1381 tgtcgctcaa gaagcgcggc ggcgagggca acggcgcggt gacggccgcc gacatcgagc
     1441 cggcgcacga cgtggagatc gtgaatcccg atcacgtgat tgcgcacctc gccgccggcg
     1501 gcaagctcga gatgcagatc aaagtcgagt cgggccgcgg ctatgtcccc ggtaacatgc
     1561 gctatctgcc cgaagagacc aagggcatcg gccggattat tctggacgcc tcgttcagcc
     1621 ccgtgcgccg cgtgtcctac tcggtcgagt cggcgcgcgt cgagcagcgc accgacctcg
     1681 acaagctgat catggatatc gagaccaatg gcgcgatcga gcccgaggaa gcgatccgct
     1741 attcggcgcg cgtgctcgtc gatcagctct cggtgttcgc acagctcgaa ggcacggcgc
     1801 tcccgaccga gcagccgaag tcgcccgcgg tcgatccgat cctgctcagg ccggtggacg
     1861 acctcgagct cacggtgcgc tcggcaaact gcctcaaggc cgagaacatc tactacatcg
     1921 gcgacctcat ccagcgcacc gagaccgagc tcctcaagac gcccaacctc ggccgcaagt
     1981 cgctcaacga gatcaaggaa gtcctcgcct cgcgcggcct gacgctcggc atgaaactcg
     2041 aaaactggcc tcccgccggc ctcgaacatc gcgcataagc catgcggcac gcacacgggc
     2101 tgaggaaact caaccgcacc tcgagccacc ggctcgcgat gctgcgcaac atgaccgtgt
     2161 cgctgctgcg gcacgaggag attcgcacca cgctgccgaa agccaaggag ctgcgacgtg
     2221 tggtcgagcc gatgatcacg ctgggcaaga agccctcgct cgccaaccgt cgcctcgcct
     2281 tcgatcgcct gcgcgaccgc ggcatcgtcg agaagctctt tgacgacctc ggcccgcgct
     2341 acgccaagcg caacggcggc tacctccgca tcctgaagat tggcttccgc aacggcgaca
     2401 acgcgcccat ggcgctcgtc acgctgatgg accggcccga ggaatcagcg gagcccaagc
     2461 ccgaggaaaa aaagaagaaa ggcgagcaga agacgaccga cgagaccaaa gcggccaaag
     2521 ccgcctgatc gttctcttca cggatttcgg gtcgcgcgac cttta
//
LOCUS       DASUNT010000536         3464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1035172, whole genome shotgun sequence.
ACCESSION   DASUNT010000536 DASUNT010000000
VERSION     DASUNT010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035172"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFX94_10825"
     CDS             <1..273
                     /locus_tag="VFX94_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768718.1"
                     /translation="DKLLEGLAGIEYNAGCWVFRGVVARIQASTQVSSTAFIFQIEFN
                     GIGALGTDEVVGILRRNVPGYSVTNPAESRLAPADARPRLPFDQVY"
     gene            278..1567
                     /locus_tag="VFX94_10830"
     CDS             278..1567
                     /locus_tag="VFX94_10830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5768719.1"
                     /translation="MKRFVPLFLLLLSMPLAGQGVLPVDRIVAVVNKDVITATELGEA
                     VAAAQRQLRRQKTPLPERGLLERQMLERLILDKAQLQLARDKGIRIDEVQLDRAVQRV
                     VQNNNMSLADFRGALERDGVSFQAWREDLREQMVLSRVREREVDDRIQVSDTEIDLFL
                     AETQAKPDTRVEYQISHILVRVPEQASPERIEAARARAQKALAEVRAGADFATLAASY
                     SDAPDALQGGAVGWRSHDRLPELFSSALARMQPGAVSEVLRSPAGFHLLKLNERRGAA
                     LENAPVAQTRLRHILIRASETMSEGEALRRLNDLRARIVSGAADFGEMARVHSGDATA
                     ARGGELEWVYPGDTVPDFERAYQELKIGEISQPVRTPFGYHLIQVLERRSADVSPERR
                     RTQARQALRERKADEAYQDWLRQLRDQVYVELRLEER"
     gene            1630..2466
                     /gene="pdxA"
                     /locus_tag="VFX94_10835"
     CDS             1630..2466
                     /gene="pdxA"
                     /locus_tag="VFX94_10835"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX5768720.1"
                     /translation="MIGAQQFAARVVAIGDRSLLDFPEVAHVPLRRPCAPGKLDPANS
                     RYVLEVLDRAIDGCLSGEFDAMVTAPVQKSVINDAGIVFTGHTEYLAERAGAAHVVMM
                     LVGGGLRVALATTHLALADVPRAVTKELLVNTLGVVDADLKKRFRIPQPRILVAGLNP
                     HSGESGHFGTEDFQVIAPAVAQARAAGIDASGPLPADTIFVPARVSQADCVLAMYHDQ
                     GLPVLKYASFGRGVNVTLGLPFVRTSVDHGTALDIAGTGKADPGSLIEALNLAIELAS
                     SA"
     gene            2438..3181
                     /gene="rsmA"
                     /locus_tag="VFX94_10840"
     CDS             2438..3181
                     /gene="rsmA"
                     /locus_tag="VFX94_10840"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX5768721.1"
                     /translation="MPSSLHRPRKRFGQHFLHERRVLARIVDAIAPAADDFIVEIGPG
                     EGALTEPLLERVQRLEVIELDRDLAAALQQKYPDRLGVHCADALEFDFARFPSGVRLL
                     GNLPYNISTALLFHLARDAKRVRDMHFMLQLEVVERMVAAPSTPAYGRLSVSLQARFR
                     MKKLFNVARGAFRPPPKVESALVRMEPLDCELPVDEHLLRRAFSARRKTLRNALPDVD
                     FAALGIDPGLRPENLSPLDYARLSIQSGG"
     gene            complement(3178..>3464)
                     /locus_tag="VFX94_10845"
     CDS             complement(3178..>3464)
                     /locus_tag="VFX94_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5768722.1"
                     /translation="RPEQKYSLAFVGYDTEDRASVIELTYNYGVERYDLGGGYGHVAL
                     EVDDAARTCEAVRAKGGKVTREAGPVKGGTTVIAFVEDPDGYKIEFIERG"
BASE COUNT          518 a         1199 c         1186 g          561 t
ORIGIN      
        1 gacaagttgc tggagggcct ggcaggcatc gagtacaacg cggggtgctg ggtgttccgc
       61 ggtgtcgtcg cgcggatcca ggcgtcaacc caggtaagct ccacggcatt catcttccaa
      121 atcgagttca atggcatcgg cgccctgggt accgacgagg tggtcgggat actgaggcgc
      181 aacgtgcctg ggtactcggt gaccaatccg gccgaatcgc ggctcgcccc ggccgacgct
      241 cgtccgcggc tccccttcga ccaggtgtat tgagagcatg aagcgctttg tccctctctt
      301 tctcctgctc ctttccatgc cgctcgcagg gcaaggcgtc cttccggtcg atcgcatcgt
      361 ggcggtggtg aacaaggatg tgatcaccgc caccgagctc ggcgaggcgg tggcggccgc
      421 gcagcggcag ctgcgccggc aaaagacgcc gctccccgag cgtgggctgc tcgagcgcca
      481 gatgctcgag cggctgatcc tcgacaaggc gcagctgcag ctcgcgcgcg acaagggcat
      541 ccgcatcgac gaggtgcagc tcgaccgcgc ggtgcagcgc gtggtgcaga acaacaacat
      601 gagcctcgcc gatttccgcg gcgcgctgga gcgcgacggc gtctcgttcc aggcgtggcg
      661 cgaagacctg cgcgagcaga tggtgctctc gcgcgtgcgc gagcgcgagg tggacgaccg
      721 cattcaggtg agcgacaccg agatcgatct ttttctcgcc gagacgcagg cgaagcccga
      781 cacgcgcgtc gagtaccaga tctcgcatat cctggtgcgc gtgccggagc aggcgagccc
      841 cgagcgcatc gaggcggcgc gcgcccgcgc gcagaaggcg ctcgccgaag tgcgcgccgg
      901 cgccgatttt gcgacgctcg cggcgagcta ctccgacgcg ccggacgcgc tgcagggcgg
      961 cgcggtcggc tggcgcagcc acgaccggct gcccgagctc ttttccagcg cgctcgcccg
     1021 catgcagccc ggcgcggtga gcgaggtgct gcgcagcccg gcgggctttc acctgctgaa
     1081 gctcaacgag cgccggggtg ccgcgctcga gaacgcgccg gtggcgcaga cgcgcctgcg
     1141 ccatatcctg atacgggcga gcgaaaccat gtccgagggg gaggcgctcc ggcgcctgaa
     1201 cgacctgcgc gcgcgcatcg tcagcggcgc cgcggacttc ggcgaaatgg cgcgcgtgca
     1261 ttccggcgac gccacggcgg cgcgcggcgg cgagctcgag tgggtctatc ccggcgacac
     1321 cgtgccggac ttcgagcgcg cctaccagga gctcaagatc ggcgagatca gccagccggt
     1381 gcgcacgcct ttcggctacc acctgatcca ggtgctggag cggcgctcgg ccgatgtgtc
     1441 gcccgagcgg cgccggacgc aggcgcgcca ggcgctgcgc gagcgcaagg ccgacgaggc
     1501 ataccaggac tggctgcgcc agctccgcga ccaggtctac gtcgagctca ggctggagga
     1561 gcgataaggc gcccggtcgt cgcgctgagc tccggagagc ccgccggcat cggccccgag
     1621 ctctgccgga tgatcggcgc gcagcagttc gccgcacgcg tagtggcaat cggcgatcgc
     1681 tcgctgctcg actttccgga ggtagcgcac gtgccgctgc gccggccgtg cgctccggga
     1741 aagctcgacc ccgccaacag ccgctacgtg ctcgaggtgc tcgaccgggc gatcgacggc
     1801 tgcctttccg gcgagttcga cgccatggtc accgcgccgg tgcagaaaag cgtgatcaac
     1861 gacgccggca tcgtctttac cggccatacc gaatatctgg ccgagcgcgc cggcgccgcg
     1921 cacgtcgtga tgatgctcgt cggcggcggg ctgcgcgtcg cgcttgccac cacgcatctc
     1981 gccttggccg acgttccgcg cgccgtcacc aaagagctcc tggtaaacac cctgggcgtg
     2041 gtggacgccg acctgaagaa gcgctttcgc attccgcagc cccggatcct cgtcgccggc
     2101 ctgaatccgc acagcggcga gtcgggccac ttcggcaccg aggacttcca ggtcatcgct
     2161 cccgcggtgg cgcaggcgcg cgccgccggt atcgatgcaa gcggcccgct gccggccgac
     2221 acgatcttcg ttccggcgcg cgtcagccag gccgactgcg tgctcgcgat gtaccacgac
     2281 caggggctgc cggtcctcaa gtacgcctcc ttcggccgtg gcgtcaacgt gacgctcggg
     2341 ctgccgttcg tgcgcacgtc ggtcgaccac ggcacggcgc tcgacatcgc gggcacgggc
     2401 aaggccgacc cgggcagcct gatcgaggca ctgaaccttg ccatcgagct tgcatcgtcc
     2461 gcgtaagcgc ttcggccagc atttcctgca cgaacgccgg gtgctcgcca ggatcgttga
     2521 cgcgattgcg ccggcagcgg atgacttcat cgtcgaaatc gggccgggcg agggcgcgtt
     2581 gaccgagcct ctgttggagc gggttcagag gctcgaagta atcgagcttg accgcgacct
     2641 tgcggcggcg ctgcagcaga aatatcccga caggctcggc gtgcattgcg cggatgcgct
     2701 cgagttcgat ttcgcccgct ttccatccgg ggtgcgcctg ctgggcaacc tgccttacaa
     2761 catatcgacc gcgctccttt tccatctcgc ccgcgatgcg aagcgggtgc gcgacatgca
     2821 cttcatgctg cagctcgaag tggtagagcg catggtggcg gcgccgtcga ctccggccta
     2881 tgggcggctt tcggtttcgc tccaggcgag gttccgcatg aagaagcttt tcaacgtggc
     2941 gcgcggcgcg ttccgcccgc cgcccaaggt cgagtcggcg ctcgtcagga tggagccgct
     3001 cgactgcgag ctgccggttg acgagcacct gctgcgacga gccttttccg cgcggcgcaa
     3061 gacgctgcgc aacgcgctgc ccgatgtcga cttcgccgcg ctcggcatcg atcccggcct
     3121 gcggcccgag aacctttctc ccttggatta cgcgcgtctt tccatccagt ccggcggcta
     3181 gccgcgctcg atgaactcga tcttgtagcc gtcggggtcc tcgacgaagg cgatcacggt
     3241 cgtgccgccc ttgaccgggc cggcttcccg cgtgaccttg ccgcccttgg cgcgcaccgc
     3301 ctcgcaggtc ctcgctgcgt catccacctc cagcgccacg tggccgtagc cgccgcccag
     3361 gtcgtagcgc tcgacgccgt agttgtaggt gagctcgatc accgaggcgc ggtcctcggt
     3421 gtcgtagccg acgaaggcga gcgagtactt ctgctccggg cggt
//
LOCUS       DASUNT010000537         5152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_423022, whole genome shotgun sequence.
ACCESSION   DASUNT010000537 DASUNT010000000
VERSION     DASUNT010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423022"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..657
                     /locus_tag="VFX94_10850"
     CDS             34..657
                     /locus_tag="VFX94_10850"
                     /inference="COORDINATES: protein motif:HMM:NF015317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HEX5768723.1"
                     /translation="MARQEHDTLLARLIGRSRLIVVVAALAVLLAAFSLFLLGAWLAL
                     GTVWSTWSGVVSGSVGSTEVIIRFLEIVSVMLKAVFFYLIGVGFYSLFISPLNVTVAL
                     GVETLNDLETKIISVIIVIMAVAFLEHFIRWEAPLEVLYHAAALGIAVAALVFFKIHT
                     NREGQEARMRRDPDLAKVQMFQRSHEEHDVADDAPDHTRRGAGGEQR"
     gene            827..1387
                     /locus_tag="VFX94_10855"
     CDS             827..1387
                     /locus_tag="VFX94_10855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010811772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX5768724.1"
                     /translation="MTTIIGISGSTRRGSLNSALLRAIAAQMPQGTELRVESIAAIPL
                     YNGDEEAAHGIPEPVARLKDAIASAEGLLLVTPEYNNSVPGVAKNAIDWLSRPPADIG
                     RVFGGKRVAIAGASPGGFGTVLSQNAWLPVFRTLGAELWPGGRLLVSRAASVFDASGE
                     LKDDAVRENVRKFVAGFVAYVKGKLG"
     gene            1481..1639
                     /locus_tag="VFX94_10860"
     CDS             1481..1639
                     /locus_tag="VFX94_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768725.1"
                     /translation="MSDPKQAAQGSAATAQQSPRFSEMKPLEKLKFVLKVAICMISFG
                     FIYPHILE"
     gene            complement(1646..2164)
                     /locus_tag="VFX94_10865"
     CDS             complement(1646..2164)
                     /locus_tag="VFX94_10865"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5768726.1"
                     /translation="MRRLLFCFTLACSLFLPPALARDVPYVPTPEKVVERMLEIAQVG
                     PNDVVYDLGSGDGRIVIAAARKHGARGVGIDIDPDRVKEARANARSAGVSDRVEFREA
                     DLFKTDLGEATVVTLYLLSSVNLQLRPKLLELKPGTRIVSHAFDMGDWKPLKTEKVGT
                     STVYYWVVPERK"
     gene            2239..4089
                     /locus_tag="VFX94_10870"
     CDS             2239..4089
                     /locus_tag="VFX94_10870"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX5768727.1"
                     /translation="MFDLVTKYKRVIQVFLGLIAITFATWGIESYTQFRSSRDTVATV
                     NGMEISQREFGEEMRRQQEQMRRMFGGAIDPAMLDTPESRRLVLENLISQRLIAREVG
                     RAHMFMSREAVIDAITSAAEFQEDGKFSAAKYSAYLASRGVSDQGNVAELQTRLPLAR
                     FAGSVSETAIAPRTVAVRLAAIEAQKREVSEARISEEQFLQQVKIDEAQVKAHYDSHL
                     ADYRTPERVRAEYLVLSADALGRQDPPTEAEIKAAYEARASQFRVEEQRRASHILVKT
                     KEEADKILQELKKNPGRFAELAKKESQDQGSAEKGGDLGWFGRGMMVKPFEDAVFKLA
                     QNDVQVVESEFGFHVVRLTGIQAAKARPYEEVRKELGDELVRQKGQRKFAEAAENFSN
                     LVYEQPESLKPAAERFKLQVQTTGWIGKSARQELGALDNPKLLSALFSPDAIKNRRNT
                     DAIEVAPSTLVAARVLEHQPAAQRAFDEVKGEIAEQLRRREASALALKEGQAKLEQLR
                     KGEDAGVKWSAPRVVSRRDAQGVPANVLRQVVAADTSKLPSFVGVPIPDGGYVLLRIS
                     RVIDEPAKEDDPQIAARFAQIYGAAQYEAMVESLRASADIEINAANLEKK"
     gene            complement(4127..4936)
                     /gene="fabI"
                     /locus_tag="VFX94_10875"
     CDS             complement(4127..4936)
                     /gene="fabI"
                     /locus_tag="VFX94_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX5768728.1"
                     /translation="MALLPGRKILVTGVISNRSIAYGVAKAARREGADIALTYQNERF
                     KDRVTDMAKELGSDIALPCDVANDREIEELFSRLGQRWQHLDGLVHAIAFAPREAIAG
                     DFLDGMTRDAFRQAHDISSYSFAALAKGALPLMQGRKASLVTLTYLGAERVVPNYNTM
                     GLAKASLEASVRYLAASLGPRGIRVNGISAGPIKTLAAAGIGGFGKILKFVEEHAPLR
                     RNVTTEDVGNAAAFLLSDLAGAITAEILYVDGGFSHVVAGIPSSASSGTEA"
     gene            4935..>5152
                     /locus_tag="VFX94_10880"
     CDS             4935..>5152
                     /locus_tag="VFX94_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768729.1"
                     /translation="MRPIILSGVRILLMLMVLVAGCGEVWNDPYPRSERNANVLYAAF
                     VQRPKHLDPVQSYTVDESLFTRQILEPPL"
BASE COUNT          883 a         1666 c         1762 g          841 t
ORIGIN      
        1 cggtgatcgc tgcgccggcc cgctgagcgc ccaatggcca ggcaggagca cgatacgctc
       61 ctcgcgcggc tgatcggccg ctcgcgcctt atcgtagtcg tcgccgcgct ggcggtgctg
      121 ctcgcggcgt tctcgctctt cctgctcggc gcgtggctcg cgctaggcac cgtgtggagc
      181 acgtggtccg gcgttgtgag cggaagcgtc ggcagcaccg aggtgatcat ccggttcctc
      241 gagatcgtca gcgtgatgct gaaggcggtg ttcttctacc tgatcggcgt ggggttctac
      301 agcctgttca tctcgcccct caacgtgacg gtagcgctcg gggtcgagac gctgaacgat
      361 ctcgagacca agattatcag cgtcattatc gtgatcatgg cggtggcgtt cctcgagcac
      421 ttcatccgct gggaagcgcc cctcgaagtc ctgtaccacg cggccgcgct cggcatcgcc
      481 gtcgccgcgc tcgtgttttt caagattcac accaaccgcg aaggccagga agcgcgcatg
      541 cggcgcgatc cggatctggc gaaggtgcag atgttccagc gctcgcacga ggagcatgat
      601 gtggccgacg acgcgccgga ccataccagg cgaggggcgg gcggcgagca gcgctgaccc
      661 atgctgaact gcgggaaact ggcgtttccc caaccgggag accaaccact cgaagtccgc
      721 gccgacggcg cccgcccgag ccgcagcatg cggcccgaat ccttaacgcc cgcttaagga
      781 ggctggtcgc gcgccgtcga tcatgcgttt gcgctaatgt ctgtccatga cgaccatcat
      841 tggaatctca ggcagcacgc ggcgcgggtc gctgaactcc gcgcttctgc gcgcgattgc
      901 ggcccaaatg ccgcagggca ccgagctgcg cgtggaatcg attgctgcga ttccgctcta
      961 caacggtgac gaggaggccg cgcacggcat cccggagccg gtagcgcgtc tcaaggacgc
     1021 catcgcttcg gccgagggtc tgcttctcgt gacgccggaa tacaacaaca gtgtgcccgg
     1081 cgtggcgaag aacgcgatcg actggctctc gcggccgccg gccgacatcg ggcgcgtgtt
     1141 cggcggcaag cgggtggcga ttgccggcgc ctcccccggc ggcttcggca cggtgctgtc
     1201 ccagaatgct tggctgccgg tgttcaggac gctgggcgcc gagctctggc cgggcggacg
     1261 gctgctcgtc tcgcgcgccg cgtcggtgtt cgacgccagc ggcgagctca aggacgacgc
     1321 cgtgcgcgag aacgtacgca agttcgtcgc gggcttcgtt gcctacgtaa aaggcaagct
     1381 cggataaacg acgtacaatc ggcgcgcgag ctcaaggggc ggcatactcc cgcggcaagc
     1441 ctgccggcgc ccccgctcgc atgctcatca ggagggcaac atgtccgacc cgaagcaagc
     1501 tgcgcagggg agtgcggcga cggctcagca gtctcccagg ttcagcgaga tgaagccgct
     1561 cgagaagttg aaattcgtgc tcaaggtggc gatctgcatg atctcgttcg gcttcatcta
     1621 cccgcacatc cttgagtaga cgtctctact tgcgctccgg cacgacccag tagtagacgg
     1681 tgctcgtgcc gaccttctcg gtcttgagcg gcttccagtc gcccatgtcg aacgcatgcg
     1741 acacgatccg ggtgccgggc ttgagctcga ggagcttcgg ccgcagctga agattcacgc
     1801 tggagagcag gtagagcgtc acgacggtag cctcgccgag gtcggtcttg aagagatcgg
     1861 cctcgcggaa ctcgacgcgg tccgaaaccc cggcgctgcg cgcgttcgcg cgcgcctcct
     1921 tgacccggtc cgggtcgatg tcgatgccca cgccgcgcgc gccatgcttc ctcgcggcag
     1981 cgatcacgat gcgcccgtcg cccgagccga ggtcgtacac cacgtcgttc ggcccgacct
     2041 gggcgatctc gagcatgcgt tcaacgacct tttccggggt cggaacgtac ggcacgtcgc
     2101 gcgccagggc aggcggcagg aagagggagc aggcgagggt gaagcagaag agcaggcgtc
     2161 gcatggattc tccagggcag taggctctct cgctcagaca agccggtaga atccgggttc
     2221 ttacgtccga gccggcccgt gttcgatctc gtcaccaagt ataagcgcgt catccaggtt
     2281 ttcctcggcc tgatcgccat cacgttcgcc acgtggggca tcgagtccta tacacagttt
     2341 cgctcctcgc gcgacacggt ggcgacggtg aacgggatgg agatctcgca gcgcgagttc
     2401 ggcgaggaga tgcgccggca gcaggagcag atgcgccgca tgttcggcgg ggcgatcgat
     2461 ccggccatgc tcgacacgcc tgaatcgcgg cgcctggtgc tcgagaatct catctcgcag
     2521 cgccttatcg cccgcgaagt ggggcgggcg cacatgttca tgagccgcga ggcggtgatc
     2581 gatgccatca ccagcgctgc ggagttccaa gaggacggca agttctccgc cgcgaagtat
     2641 tccgcgtacc tcgcgagccg cggcgtttcc gatcagggca acgttgccga gctgcagacc
     2701 cggctgcccc tcgcgcgctt cgccggctcg gtgtcggaga ccgccatcgc gccgcgcacc
     2761 gtggcggtgc gcctcgccgc gatcgaggcg cagaagcgcg aggtgtcgga ggcgcgcatt
     2821 tccgaggagc aatttctcca gcaggtgaag atcgacgagg cccaggtcaa ggcgcactac
     2881 gactcgcacc tcgccgacta tcgcaccccg gagcgggtgc gcgccgagta tctggtcctt
     2941 tcggccgatg cgctggggcg ccaggatccg ccgaccgaag ccgagatcaa ggcagcctac
     3001 gaggcgcgcg cgagccaatt ccgcgtcgag gagcagcgcc gcgcgagcca catcctggtc
     3061 aagacgaagg aggaagccga caagatcctt caggagctga agaagaaccc cgggcgcttc
     3121 gccgagctcg ccaagaagga gtcccaggat caggggtcgg ccgaaaaggg cggcgacctc
     3181 ggctggttcg gccgcggcat gatggtgaag cccttcgagg atgcggtgtt caagctcgcg
     3241 cagaacgacg tgcaggtggt ggagagcgag ttcggcttcc acgtcgtgcg cctgaccggc
     3301 atccaggccg cgaaagcgcg tccgtacgag gaggtgcgca aggagctcgg cgacgagctc
     3361 gtgcgccaga agggacagcg caagttcgcc gaggcggcgg agaacttcag caacctggtg
     3421 tacgagcagc cggagagcct gaagcccgcc gcggagcgct tcaagctgca ggtgcagacc
     3481 acgggctgga tcggcaagtc ggcgcgccag gagcttgggg cgctggacaa cccgaagctg
     3541 ctgtcggcgc tcttctcccc ggatgcgatc aagaaccgcc gcaacaccga cgcgatcgag
     3601 gtggcgccca gcacgctcgt cgcggcgcgc gtgctcgagc accaacccgc cgcgcagcgc
     3661 gccttcgacg aagtgaaggg cgagattgcc gagcagctgc gccggcgcga ggcctcggcg
     3721 ctggcgctga aggaaggcca ggcgaagctc gagcagctgc gcaagggcga ggacgccggc
     3781 gtgaaatgga gcgcgccgcg cgtggtctcg cggcgcgatg cgcagggagt gccggccaac
     3841 gtgctgcgcc aggtggtggc cgccgatacg agcaagctgc cctcgttcgt cggcgttccg
     3901 attccggacg gcggctacgt gctgctacgt atctccaggg tcatcgacga gcccgcgaag
     3961 gaagacgatc cgcagatcgc cgcccgcttc gcgcagatct atggcgcggc acagtacgag
     4021 gcgatggtgg agagcctgcg cgcgagcgcc gacatcgaga tcaacgcggc caaccttgag
     4081 aagaagtagc caagaaaagg ggtcctgacc ccttttcttc ttttcttcag gcttccgtgc
     4141 ccgagcttgc cgaggagggt atcccggcca cgacgtggct gaagccgccg tccacgtaca
     4201 ggatctccgc ggtaattgcg cccgccagat ccgagaggag gaacgcggcg gcgttgccga
     4261 cgtcctcggt ggtgacgttg cgccgcagcg gggcatgctc ctcgacgaac ttgaggatct
     4321 tgccgaagcc gccgatgccg gcggcggcga gcgtcttgat cgggccggca gagatgccgt
     4381 tcacccggat gccgcgcggc ccgaggctcg cggcaaggta gcgcaccgag gcctcgaggc
     4441 tcgccttcgc gagccccatc gtgttgtagt tcggcacgac gcgttcggcg ccgagatagg
     4501 tgagcgtgac gagagaagcc tttcgtccct gcatgagcgg cagcgcgccc ttcgccagcg
     4561 cggcgaagct gtacgaggag atgtcatgcg cctggcggaa tgcatcgcgc gtcattccgt
     4621 ccaggaaatc gcccgcgatc gcctcgcgcg gcgcaaacgc gatcgcgtgc accagcccat
     4681 cgagatgctg ccatctttgc ccgagccggg aaaaaagctc ctcgatctcc cgatcgttcg
     4741 ccacgtcgca gggcagcgcg atgtcggagc cgagctcctt cgccatgtcg gtgacgcggt
     4801 ccttgaagcg ctcgttctgg taggtgagcg cgatatccgc gccttcgcgc cgcgcggcct
     4861 tcgccacccc gtaggcgatc gagcggttgg agatgacgcc ggtgacgagg atcttcctgc
     4921 cggggagaag cgccatgcgg ccgattatac tgagcggcgt gcgcatcctt ctgatgctca
     4981 tggtgcttgt cgcgggctgc ggcgaggtct ggaacgatcc ttatccgcgc tcggagcgaa
     5041 acgcgaacgt cctgtacgcg gcgttcgtgc agcggccgaa gcacctggac ccggtgcagt
     5101 cctacaccgt cgacgagtcg ctcttcacgc gccagatcct cgagccgccg ct
//
LOCUS       DASUNT010000538         2526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_423142, whole genome shotgun sequence.
ACCESSION   DASUNT010000538 DASUNT010000000
VERSION     DASUNT010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423142"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VFX94_10885"
     CDS             <1..1054
                     /locus_tag="VFX94_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5768730.1"
                     /translation="KQQIRFCRSFDGTRIAFAVTGHGPADALPLVRAPHWLTHLEYEW
                     QSPIWRPWLTALSSGRAVVRMDERGCGLADRAVETISFEAFVRDLEAVVDAAGLERFA
                     LFGHSQGGAIALEYAVRHPERVAYLVLLGAYARGRFKRSPESRSEAEAQLKLVEFGWG
                     GDDPSYRQFFASQFMPGATLEQLRQMAELQRVSSSPADALRILRAFYDIDVSASVPRI
                     GCPTLVLHARGDLRVSFEEGRAVAAMIPGARFVPLESENHILLEHEPAFACVFEELRE
                     FLGSRQSFGGLTAREAQILEHIAQGLDNAQIAARLDLSEKTVRNHITSIFDKISVESR
                     AQAIVKAREQGLGQRS"
     gene            1149..1907
                     /locus_tag="VFX94_10890"
     CDS             1149..1907
                     /locus_tag="VFX94_10890"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEX5768731.1"
                     /translation="MGALAFLYGAVSYALFLGVFLYAIAFVGNLPVPKTIDSGASAGL
                     VESILVNVVLLGLFAVQHSVMARPAFKRWWTRIVPRSVERSTYVLLASLALALLYWQW
                     RPMTGLVWSVEQPAGKLALYALFGLGWTIVLLSTFMINHFDLFGLRQVWLRVRGADYT
                     EVPFVTRGFYNFVRHPIMLGFLIAFWATPHMTTGHLLFAIATTGYIFIGIFFEERDLA
                     AAHGRSFEQYRREVPMILPVPRRSEESKRAMSAR"
     gene            complement(2216..2449)
                     /locus_tag="VFX94_10895"
     CDS             complement(2216..2449)
                     /locus_tag="VFX94_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768732.1"
                     /translation="MEYILYFSPSPAPQNLAQEHLDRLIPMRFSSEKDALHGAVLVMR
                     GGQHPWLIAGPGVVLDAQEIAARCEPILRLFRR"
BASE COUNT          374 a          853 c          843 g          456 t
ORIGIN      
        1 gaagcagcag atccgcttct gcaggagctt cgacggcacg cgtatcgcct ttgctgtcac
       61 cgggcacggt ccggccgatg cgctgccgct ggtgcgcgcg ccgcactggc tgacccacct
      121 ggaatacgag tggcagagcc cgatctggcg gccgtggctg accgcgcttt cgagcgggcg
      181 cgcggtggtg cgcatggacg agcgcggctg cgggctggcc gaccgcgccg tcgagaccat
      241 ctcgttcgag gcgttcgtgc gcgacctgga agcggtggtc gacgccgcgg ggctcgagcg
      301 ctttgcgctt ttcggtcatt cacagggcgg cgcgatcgcg ctggaatacg cggtgcgcca
      361 tcccgagcgc gtcgcgtacc tcgtgctgct cggcgcctat gcgcgcggcc ggttcaagcg
      421 ctcgccggaa agccgcagcg aagccgaggc gcagctcaag ctggtcgagt tcggctgggg
      481 cggcgacgat ccttcgtacc gccaattctt tgccagccag ttcatgccgg gggcgacgct
      541 cgagcagctg cggcagatgg ccgagctgca gcgcgtgtcg tcctcgccgg ccgacgcgtt
      601 gcgcatcctg cgcgcgttct atgacatcga cgtgtcggcg tcggtgccgc gcatcggctg
      661 cccgaccctg gtgctgcatg cgcgcggcga cctgcgcgtt tcgttcgagg aggggcgcgc
      721 cgtcgcggcg atgattccag gcgcgcgctt cgtgccgctc gagtcggaga atcacatcct
      781 gctcgagcac gagccggcat tcgcgtgcgt gttcgaggag ctgcgggaat ttctcggcag
      841 ccggcaaagc ttcggcggcc ttaccgcgcg cgaggcgcag atcctggagc acatcgcgca
      901 gggcctcgac aacgcccaga tcgccgcccg gctcgatctg tccgagaaga ccgtgcgcaa
      961 ccacatcacc agcatcttcg acaagatcag cgtcgagagc cgcgctcagg caatcgtgaa
     1021 ggcgcgggag cagggtctgg gacagcggtc ctagcctttc aggcctcgcc cggcgtaccc
     1081 gggaccagcg ccccgtgcga cgctctcaac cctccggcag gatagagccc ctaacggagg
     1141 gtcacttcat gggcgcattg gcattcctgt acggcgcagt cagctacgcg ctctttctcg
     1201 gcgtctttct gtacgccatc gccttcgtgg gcaacctgcc ggtgcccaag accatcgact
     1261 ccggagcctc cgcggggctg gtggaatcaa ttctggtgaa cgtggtgctg ctcggcctgt
     1321 tcgcggtcca gcacagcgtg atggcgcggc cggcattcaa gcgctggtgg acgcggatcg
     1381 tgccgcgctc ggtcgaacgc agcacctacg tcctgcttgc cagccttgcg ctggcgttgc
     1441 tctactggca gtggcgcccg atgaccggat tggtttggag cgtcgagcag ccggcgggca
     1501 agctcgccct ctacgccctg tttggcctgg gctggaccat cgtgctgctg tcgaccttca
     1561 tgatcaacca cttcgatctg ttcgggctgc ggcaggtgtg gctgcgcgtg cgcggcgccg
     1621 actacaccga ggtgccgttc gtcacgcgcg gcttctacaa cttcgtccgc cacccgatca
     1681 tgctggggtt cctcatcgcc ttctgggcga cgccgcacat gacgacgggg catctgctgt
     1741 tcgccatcgc caccaccggc tacatcttca tcggcatctt cttcgaggag cgcgacctcg
     1801 ccgccgcgca tgggcgcagc ttcgagcagt accggcgcga ggtgccgatg atcctgccgg
     1861 tgcctcgcag gtccgaagaa agcaagcgcg cgatgagcgc gcgttgacgt gtcatccgcg
     1921 cctggcgttc gggtcccggc ccggcggccg ttgtccctcg ctgggcgact ggtccgaaag
     1981 caggtcgacc agcctgagga acagcgcatc cggaactggc gcggcgccgc tcaggtaaag
     2041 cttcatcgct cgttgagtca cacctagcct cttcgcaaga ttctctacgc cgccaagccg
     2101 tgcggccgcc tgttcgagca gccgttgtct tgcttctggt gtggacatgg cacccccgca
     2161 gcatcgcagt ctcgctgctc gcagccctta cccgcaatcg gggggaacct tagactcaac
     2221 gcctgaagag ccgcaggatt ggctcgcagc gcgcggcgat ctcctgcgca tcgagcacta
     2281 cgcccggccc ggcgatcagc cagggatgct ggccgccgcg catcaccagc accgcgccgt
     2341 gcagcgcatc cttctcgctg ctgaagcgca tcgggatcag ccgatcgaga tgctcctgcg
     2401 cgaggttctg cggggcgggt gaggggctga agtaaaggat gtactccatc gagcagtttc
     2461 tacgccgcgc cgcacgccgt atcttgcggt gccggacaac ccgagtagtc tgcgaggacc
     2521 aatgcg
//
LOCUS       DASUNT010000539         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_247628, whole genome shotgun sequence.
ACCESSION   DASUNT010000539 DASUNT010000000
VERSION     DASUNT010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247628"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFX94_10900"
     CDS             complement(<1..237)
                     /locus_tag="VFX94_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX5768733.1"
                     /translation="MQVRRIAGALGAEILGVDLSRDIPAPEIRRVFLEHQVIFLRGQK
                     LDPAQFMAFARTMGKPVPYPFVPGIEGFPEVIEVK"
     gene            complement(238..1017)
                     /locus_tag="VFX94_10905"
     CDS             complement(238..1017)
                     /locus_tag="VFX94_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5768734.1"
                     /translation="MDLGLHGKTALVCAASKGLGKGCAMALAREGVNLVITARGAEAL
                     ESTADEIRNEWRVKVTAVAGDITTAQGREAALKAAPAPDILVNNAGGPPPGDFREWDR
                     ETWVKAVDANMITPILLIRAVVDGMIARKFGRIVNVTSGAVKAPIPELGLSNGARTGL
                     TGFIAGLSRTTVRHNVTINNLLPGPFETDRLRANLEFNAKKLGRTADELRKQRTDANP
                     AGRFGTIEEFGDACAYLCSAQAGFITGQNVLMDGGAYPGTF"
     gene            complement(1020..1922)
                     /locus_tag="VFX94_10910"
     CDS             complement(1020..1922)
                     /locus_tag="VFX94_10910"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5768735.1"
                     /translation="MTRSSQTSYRAWGILFALIGVLAFSFRPILIKLSYGAHPVSPVT
                     LLFLRMSLSLPFFLAIGWWLRAQEPRLRPRDWLAIAGLGFIGYYGASFLDFIGLQWVG
                     AGVGRLILYLFPTLVVLLSFLFLRKPPTWREIVALVVSYAGIALVVWNQIAGEGRLFA
                     LGAGFVFASALCYAVYLVAGSQVVKRVGSMRFTAYSTTAATVPAILQFLFAESAQSLH
                     LPAAVWGYAVVLATVSTVLPLFLQAEALNRIGANHFALMGAIGPVSVAVTSAVGLDEP
                     FTSVQALGGALVICGVLLVSLKKG"
     gene            complement(1919..>2168)
                     /locus_tag="VFX94_10915"
     CDS             complement(1919..>2168)
                     /locus_tag="VFX94_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX5768736.1"
                     /translation="AYEKLRSHVEEKQPAAAPAEPASAPNPISDILFGKTGPRGGRQT
                     QGVLEAATKSAARAMGSELGRQILRGVLGSILGSGRRR"
BASE COUNT          394 a          715 c          717 g          342 t
ORIGIN      
        1 ctttacctcg atgacctcgg ggaagccctc gatgcccgga acgaacgggt aagggaccgg
       61 cttgcccatg gtgcgcgcga acgccatgaa ctgcgccgga tcgagtttct ggccacggag
      121 gaagatgacc tggtgctcga gaaatacgcg gcggatttcg ggcgccggga tgtccctcga
      181 caggtcgacg ccgaggatct ctgcgcccaa ggcaccggcg atgcgcctga cctgcattca
      241 gaacgtaccg ggatacgcac cgccgtccat cagcacattc tggccggtga tgaaccctgc
      301 ctgagcgcta cataaatagg cgcaggcgtc gccgaactct tcgatggtgc cgaagcgccc
      361 ggcgggattc gcgtcggtgc gctgcttgcg gagctcgtcg gctgttcttc ccagcttctt
      421 cgcattgaac tccagattcg cgcgcagccg gtcggtctcg aacggtccgg gcaggaggtt
      481 gttgatggtg acgttgtgcc gcaccgtggt gcgcgagagc ccggcgatga aacccgtcaa
      541 cccggtgcgc gcaccgttcg acagcccgag ctcggggatc ggcgccttca ccgcgccgga
      601 agtaacgttg acgatgcggc cgaacttgcg ggcgatcatg ccgtcgacca cggcgcggat
      661 caggaggatc ggcgtgatca tgttcgcgtc caccgccttc acccaggtct cgcgatccca
      721 ttcgcggaaa tcccccggcg gcggaccgcc ggcgttgttg acgaggatgt ccggcgcggg
      781 ggcggctttc agcgccgctt cacgcccctg cgcggtcgtg atgtcgcctg ctaccgcagt
      841 gacttttact ctccattcgt ttcgaatctc atctgccgtc gattccaggg cctcggcgcc
      901 gcgcgcggta atgacgagat tcacgccctc tcgcgcgagc gccattgcgc aacctttgcc
      961 gagtcccttc gacgccgcgc acactagcgc cgtcttgccg tggagtccga gatccatgcc
     1021 tagcctttct tgagtgacac gagaagaacg ccgcagatga cgagcgcgcc gccgagcgcc
     1081 tgcaccgacg taaaaggctc atcgagcccg acggcgctcg tcaccgcgac gctcaccggc
     1141 ccgatcgcgc ccatcagcgc gaagtggttc gcgccgatgc ggttcagcgc ctcggcctgc
     1201 aggaagagcg gcagtaccgt gctcaccgtg gcgagcacga cggcgtagcc ccagaccgcc
     1261 gccggcagat gcagcgactg cgccgattcg gcgaacagga actggagaat tgcaggcacc
     1321 gtcgcggccg tggtggagta cgcggtgaag cgcatggatc ccacgcgctt caccacctgg
     1381 ctgccggcga cgaggtagac ggcatagcac agcgccgagg cgaacacgaa ccccgccccg
     1441 agcgcgaata gccttccttc cccggcgatc tggttccaca cgacgagcgc gatcccggca
     1501 tagctgacga cgagcgcgac gatttcacgc caggtcgggg gcttgcgcag aaagaggaag
     1561 gaaagaagca ccacgagcgt cggaaagagg tagaggatca gccggccgac gcccgcgccg
     1621 acccattgca ggccgatgaa gtcgagaaag ctcgcgccgt agtagccgat gaaccccagc
     1681 ccggcgatcg ccagccagtc gcgcggtcgg aggcgcggct cctgcgcgcg caaccaccag
     1741 ccgatcgcga gaaagaaggg cagcgagagg ctcatgcgca ggaaaaggag cgtcaccggg
     1801 ctgaccgggt gcgctccata ggaaagcttg atgaggatcg gtcggaatga aaacgccagt
     1861 actccgatca gagcgaacag aattccccaa gcgcggtatg aggtttggct gcttcgcgtc
     1921 aacggcggcg gccgctgccg aggatcgacc cgagcactcc gcgcagaatt tgccggccaa
     1981 gctccgagcc catggcgcgc gcggcgcttt tggtggccgc ctcgagcacg ccctgggtct
     2041 gccggccgcc gcgcgggccg gtcttgccga aaaggatgtc ggaaatcggg ttgggcgccg
     2101 aagcgggctc agcgggagcc gccgccggct gcttctcctc cacgtggctg cgcagctttt
     2161 cgtaagcc
//
LOCUS       DASUNT010000540         4805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_860624, whole genome shotgun sequence.
ACCESSION   DASUNT010000540 DASUNT010000000
VERSION     DASUNT010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860624"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1588
                     /gene="glgX"
                     /locus_tag="VFX94_10920"
     CDS             <1..1588
                     /gene="glgX"
                     /locus_tag="VFX94_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX5768737.1"
                     /translation="PAVPEAERGTYRALAHGDVVGYIKSLGVTTVELLPVHTFINDSF
                     LLERKLTNYWGYNSIGFFAPDPRYAADASKTLQEFKEMVARFHDAGLEVFIDVVYNHT
                     AEGNELGPTLSFKGIDNASYYRLLPDKKRHYINDTGTGNTLNLSHPVVIQMVCDSLRY
                     WAQEMHVDGFRFDLGTILAREPGGFDTYNGFMKAVVQDPLLAKVKLIAEPWDCGPGGY
                     QLGRFPAGWAEWNDQFRDTVRDYWRGEASPAALAPRLCGSPDIFNRRGRQPWASVNFI
                     TAHDGFTLYDLVTYNDKHNEANREGNRDGHSHNRSWNHGVEGPTDDEMIRGLRKRQMK
                     NMLATLLLSQGTPMLLAGDEFGRSQRGNNNAYCQDNEISWLRWVHDDRAMRLIRFVQK
                     LTGLRARYPILRRTRFLRGEFDAELGVKDVTWFNANGEDMREEHWKDGNVRCFGMLLD
                     GRAQTTGIHRRGEEATLLIIFNPWQDLVKFKLPRANGGENWKLLADTNFPDSKEEPQF
                     GFGHEYEVTARSLLLFALGQ"
     gene            1572..3251
                     /locus_tag="VFX94_10925"
     CDS             1572..3251
                     /locus_tag="VFX94_10925"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5768738.1"
                     /translation="MLSASNASSLLGLLVAAAVLYFARDVLIPLALAILVSFLLAPVV
                     RRLEYWKLGRVPSTLLAVALTTAVIGAVGYVGAREALSLAAKLPEYRENIRAKVRALR
                     ANDSGTLGKAAEAIKELEGEAAPSAAQPLAVAETPPSALAAAAEWLGPFVKPVGTALA
                     VFVLTILLLLHRENMRERLIGLIGPRQINITTQALGEASYRVSRYLSTLLLVNASFGI
                     PFGIALYFIGIPSALLWALLGTLLRFIPYVGVWIAVAMPVLLAFGIFDGWTEVTAVLG
                     TFLVLELLLVNAVEPWLYGRSAGISPIAVIAAALFWTWLWGPVGLLLAVPLTVCIAVM
                     GRYIPELGWVNTLLGVEPVLEPEARLYQRLVALDDEEAMALAHEYAREHGVAALYDSV
                     LVPALSLAESDRHAGALEPDRARFVAQTVREIIDEVAESAPQESRCVHVCIVPAHDEA
                     DELAGAMLARLLPGAQLLSSKSLAAEVLERVSDGACKLVCICAVPPHAASHAAYRARR
                     MRQRFPELKTVIALLTANGIERAKPRLLAAGASEVFTRLADAEGYARQISV"
     gene            3262..3705
                     /gene="aac(6')"
                     /locus_tag="VFX94_10930"
     CDS             3262..3705
                     /gene="aac(6')"
                     /locus_tag="VFX94_10930"
                     /EC_number="2.3.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004548791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047663 - aminoglycoside
                     6'-N-acetyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6'-N-acetyltransferase"
                     /protein_id="HEX5768739.1"
                     /translation="MKIEACESADHPGWLRLREALWPGSRAGHLAEMKLFVSQPARFR
                     QFMAYSPSSEAIGLAEASLRSDYVNGTESSPVAFLEGLYVVPQARRKGVAASLVAAVC
                     GWARAAGCRELASDAALENAISQVVHRALGFEETERVVFFRWVLE"
     gene            3702..4088
                     /locus_tag="VFX94_10935"
     CDS             3702..4088
                     /locus_tag="VFX94_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEX5768740.1"
                     /translation="MIRIVRLGTPRVRGEGLRIGAARRPPRGVKREDYARLDYYDVWL
                     PELAPSQKLVSWAQSQPLTDKRWAKFERDYRRQMTKPEAARLLELLARLSHYADFSLG
                     CYCKNERRCHRSILKQLLAGHGARIS"
     gene            4069..4587
                     /locus_tag="VFX94_10940"
     CDS             4069..4587
                     /locus_tag="VFX94_10940"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5768741.1"
                     /translation="MAHESLETPSPWVVRWAPLVARGPVLDVASGGGRHARLFAARGL
                     EVVAVDREAHVIPGVEFLRADLEDGSPWPFAGRRFGGIVVTNYLHRPLFSHLAAALDE
                     GGVLVYETFMLGNERFGKPSNPAFLLHPGELLKAFAGLTVVAFEQGATEKAVVQRLCA
                     IRGDATRARIPA"
     gene            4584..>4805
                     /locus_tag="VFX94_10945"
     CDS             4584..>4805
                     /locus_tag="VFX94_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX5768742.1"
                     /translation="MITGSLVAVVTPMHEDGRLDSGRFKQLIDWHVAEGTDGIVVVGT
                     TGESPTVDFDEHKELIRLAVQHSHGRIPVV"
BASE COUNT          765 a         1594 c         1624 g          822 t
ORIGIN      
        1 tccggccgtg cccgaggccg agcgcggcac gtatcgcgcg ctcgcccacg gcgacgtggt
       61 cggctacatc aagtcgctcg gggtgacgac cgtggagctg ctgccggtgc acaccttcat
      121 caacgacagc ttcctgctcg agcgcaagct caccaactac tggggctaca actcgatcgg
      181 cttcttcgcg ccggatccgc gctacgccgc cgatgcctcg aaaacgctgc aggaattcaa
      241 ggagatggtg gcgcgcttcc acgatgccgg gctggaagtc ttcatcgacg tggtctacaa
      301 ccacaccgcc gagggcaacg agctgggacc cacgctttcc ttcaagggga tcgacaacgc
      361 ttcgtactac cggctcctgc ccgacaagaa gcggcactac ataaatgaca ccggtacggg
      421 caacacgctt aatctgtcgc accccgtggt gatccagatg gtgtgcgaca gcctgcgcta
      481 ctgggcgcag gagatgcacg tcgacggctt tcgcttcgac ctcggcacga tcctcgcgcg
      541 cgagccgggc ggcttcgata cgtacaacgg cttcatgaag gcggtggtgc aggatcccct
      601 gctcgccaag gtgaagctga tcgccgaacc ctgggactgc ggaccgggcg gctaccagct
      661 cggacggttc ccggcgggct gggccgagtg gaacgaccag tttcgcgaca cggtgcgcga
      721 ttactggagg ggcgaagcgt cgcccgcggc gcttgcgccg cggctttgcg gctcgccaga
      781 catcttcaac cgccgcgggc gccagccgtg ggcgagcgtc aacttcatca cggcgcacga
      841 cggcttcacg ctctacgacc tcgtcacgta taacgacaag cacaacgagg cgaatcgcga
      901 gggcaaccgg gacggccatt cgcacaaccg ttcctggaac cacggcgtcg agggacccac
      961 cgacgacgag atgatccgcg ggctgcgcaa gcggcagatg aagaacatgc tggccacgct
     1021 gctgctctcg cagggtacgc caatgctgct cgccggcgac gagttcggcc gcagccagcg
     1081 cggcaacaac aacgcctact gccaggacaa cgagatctcg tggctgcgct gggtgcatga
     1141 cgaccgggcg atgcgactga tccgcttcgt gcagaagctc accggcctgc gcgcccgcta
     1201 cccgatactg cggcgcacgc gcttcctcag gggagagttc gacgccgagc tcggcgtcaa
     1261 ggacgtgacg tggttcaacg ccaacggcga ggatatgcgc gaggagcact ggaaagacgg
     1321 aaacgtgcgc tgcttcggga tgctgctcga cgggcgggcg cagaccaccg gcatccacag
     1381 gcgcggcgag gaggcgacgc tgctcatcat cttcaatccg tggcaggacc tggtgaagtt
     1441 caagctcccg cgcgcgaacg gcggcgaaaa ctggaagctg cttgccgaca ccaacttccc
     1501 ggattccaag gaagagccgc aattcgggtt cggtcacgag tatgaggtga ccgcgcgctc
     1561 gctgctgttg tttgctctcg gccagtaacg cctcctcgct cctcgggctg ctcgtcgcgg
     1621 cggcagtcct ctacttcgcg cgcgatgtgc taattccgct cgcgctcgcg atcctggtct
     1681 cgttcctgct cgcgccggtc gtgcgccgcc tggagtactg gaagctgggg cgcgttcctt
     1741 cgacgctgct cgcggtggcg ttgaccaccg ccgtgatcgg agccgtgggc tacgttggcg
     1801 cgcgcgaggc gctctcactc gccgccaagc tgccggagta tcgcgagaac atccgcgcca
     1861 aggtgcgcgc gctgcgcgcg aacgacagcg gcaccctcgg caaagccgcc gaagcgatca
     1921 aggaactgga aggcgaggcc gcgccctccg cggcgcaacc tctggcggtg gcggagacgc
     1981 cgccgagcgc gctggccgcg gccgccgaat ggcttggccc gttcgtgaag ccggtcggca
     2041 ccgctctcgc ggtcttcgtg ctgaccatcc tgctgctgct ccatcgagag aacatgcgcg
     2101 agcggctgat cggcctcatc ggcccgcgcc agatcaacat caccacccag gcgctcggag
     2161 aggcctccta ccgcgtaagc cggtatctct ccacgctgct cctggtgaac gcttcgtttg
     2221 gcatcccgtt cggcatcgcg ctgtacttca tcggcattcc gagcgcgctg ctatgggcgc
     2281 tgctcggcac gctgttgcgc ttcattccct acgtcggcgt gtggatcgcc gtcgccatgc
     2341 cggtgctgct tgcgttcggg atcttcgacg gctggaccga ggtcacggcg gttctcggga
     2401 ccttcctcgt gctcgagctt ctgctcgtga atgccgtcga gccatggctt tacggccgaa
     2461 gcgcgggaat ctcgccgatc gcggtgatcg ccgcggcgct cttctggaca tggctctggg
     2521 gcccggtcgg gctgctcctc gccgtgccgc ttaccgtgtg cattgccgtc atgggccgct
     2581 acatccccga gctgggctgg gtgaacacgc tgctcggggt cgagccggtg ctcgagccgg
     2641 aggcgcgtct ctatcagcgg ctggttgccc tcgacgacga ggaagcgatg gcgcttgcgc
     2701 acgagtatgc gcgcgagcac ggtgtcgccg cgctgtacga ctcggttctc gtcccggcgc
     2761 tttcgctcgc cgaaagcgac cgccacgccg gcgcgctcga gccggaccgg gcgcgtttcg
     2821 tcgctcagac ggtgcgcgag atcatcgacg aggttgccga aagcgcgccg caggaaagcc
     2881 gctgcgtcca cgtgtgcatc gttcccgcgc acgacgaggc ggacgagctg gccggagcga
     2941 tgctcgcgcg gctcttgcca ggggcgcagc tcttgtcctc caagagcctg gccgccgaag
     3001 tgctcgagcg ggtaagcgac ggcgcctgca agctcgtatg catatgcgcc gtgccgccgc
     3061 acgcggcgag ccacgccgca taccgcgcgc gacgcatgcg ccagcgcttt cccgagttga
     3121 agaccgtgat cgcgcttctc acggcgaacg gcatcgagcg cgcgaagcca aggctcctcg
     3181 ccgccggcgc gagcgaggtg ttcacgcgcc ttgccgacgc cgaggggtat gcccgccaga
     3241 tttccgtgta atttagcccc ggtgaagatc gaagcctgcg agtcggcgga ccacccgggc
     3301 tggctgcgct tgcgcgaggc gctgtggccc ggttcgcgcg ccggtcacct ggcggaaatg
     3361 aagctgttcg tttcgcagcc ggcccgcttc cggcaattca tggcgtactc gccctccagc
     3421 gaggcgatcg ggctggcgga ggcttcactg cgcagcgatt acgtgaacgg taccgaatcg
     3481 tccccggtcg ccttcctgga aggcctgtat gtcgtaccgc aggcgcggcg caagggcgtg
     3541 gccgcttcgc tggtggccgc ggtctgcggt tgggcgcgcg ctgccggctg ccgcgagctt
     3601 gcttcggacg cggcgctgga gaacgcgatc agccaggtcg tgcaccgtgc gctgggattc
     3661 gaggaaaccg agcgcgtagt cttcttccgg tgggtgctcg aatgataagg attgtgcggc
     3721 tcggcactcc gcgcgtgcgc ggcgaagggc tgcgcatcgg cgctgccagg cggccgccgc
     3781 gcggcgtcaa gagggaagac tatgcgcgcc tcgattacta cgatgtgtgg ctgcccgagc
     3841 ttgcccccag ccagaaattg gtgagctggg cgcagtcgca gcccctcacc gataagcgct
     3901 gggcgaagtt cgagcgcgac taccgccggc agatgacaaa gcccgaagcg gcgcggctgc
     3961 tggaactgct cgcgcggctc tcgcattacg cggatttttc gctcggctgc tactgcaaga
     4021 acgagcggcg ctgccatcgc tcgatcctga agcaattgct tgccggacat ggcgcacgaa
     4081 tctcttgaaa cgccgtcgcc gtgggtggtg cgctgggcgc cgctcgtggc gcgcggacca
     4141 gtgctcgacg tggcgagcgg gggcgggcgg cacgcccggt tgttcgcagc gcgcgggctg
     4201 gaagtggtgg cggtcgaccg cgaggcccac gtaatcccgg gggtggagtt cctgagagcc
     4261 gatctcgaag acggcagccc gtggcccttt gccggccggc gcttcggcgg catcgtcgtg
     4321 acgaactatc tgcatcggcc actcttcagc caccttgccg ctgcgctcga cgagggcgga
     4381 gtgctggtct acgagacgtt catgctcggc aatgaacgct tcggcaagcc gtccaatccc
     4441 gccttcctgc tgcatccggg ggagctcctg aaggctttcg ccggcctgac ggtcgtcgct
     4501 ttcgagcaag gagcgaccga aaaggccgta gttcagaggc tttgcgcgat ccgtggagat
     4561 gcgactcgcg ctagaatccc cgcatgatca cgggaagcct ggtcgcagtc gtcacgccga
     4621 tgcacgagga cggccgcctg gactccggcc gcttcaagca gctcatcgac tggcatgtcg
     4681 ccgagggcac cgacggcatc gtggtggtcg gcaccaccgg cgagtcgccc acggtggact
     4741 tcgacgagca caaggagctg atccggctcg cggtccagca ttcgcacggg cgtattcccg
     4801 tggtc
//
LOCUS       DASUNT010000541         5584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1123530, whole genome shotgun sequence.
ACCESSION   DASUNT010000541 DASUNT010000000
VERSION     DASUNT010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123530"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1128)
                     /locus_tag="VFX94_10950"
     CDS             complement(163..1128)
                     /locus_tag="VFX94_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5768743.1"
                     /translation="MRVRFWGTRGSLPVALTWRDVRDRLARALVAANGKNVDTLEKAH
                     AFAGQHLGFSLTHTFGGHSACVELMPPDAGPDEYFVCDMGSGARAFGEHIAARQGGRA
                     ATVYVFMSHMHWDHIMGFPFFGPAYVRGNNIRIHGCHDALEHAFRRQQDPPSFPVPFS
                     QLAADIDFIHLAPEQAHELPAVTVTPQLQHHSGDSYGFRFKSRGKSVVYTTDSEHKLD
                     DHAARKRIVEFFRGADLVIFDAMYSLAEAVSVKADWGHSSNVVGVELCQMANARRLAL
                     FHHEPANGDDAIERILRETRRLEELTRGVQRLEVIAAYDGLEIEL"
     gene            complement(1136..1507)
                     /locus_tag="VFX94_10955"
     CDS             complement(1136..1507)
                     /locus_tag="VFX94_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5768744.1"
                     /translation="MKLIQRRYADSLVLTPAGRLDHDSCDDFGATLQPLVDETRDGRA
                     LVLDLSALEYVSSAGLRLLMLGGKQARTQGGRIMIAAMRPLVAEIFQISRFTMLFEIY
                     PGVREALAAASPQAAAAFDGG"
     gene            1531..2040
                     /locus_tag="VFX94_10960"
     CDS             1531..2040
                     /locus_tag="VFX94_10960"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HEX5768745.1"
                     /translation="MALAAFNFARWIDEHAHLLRPPVGNQMVFREAEDLIVMVIGGPN
                     ARSDYHDDPYEEFFYQLRGDMVLKVIENGRPRDVPIRQGEILLVPGHCRHSPQRPAGT
                     VGLVVEKVRPREVKDGFEWYCPRCWGLVHRVDVNVQNIVTDLPPLFEAFYKSERNCPR
                     CGAVHPGKG"
     gene            2040..2828
                     /locus_tag="VFX94_10965"
     CDS             2040..2828
                     /locus_tag="VFX94_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5768746.1"
                     /translation="MDAEAAARCLFASHERRERFTPLPAHLAPRSVAQAHAIQDAFVA
                     QRAQKLGGIAGYKVALTSPQMRRFVGVDEPQAGMMLETTLHRSPARVRAADFVRLVVE
                     FEIAVQIGDDLPAADSPFSRQRVAEAVEAVMPALELADDRNADYSELARHPLELIADN
                     CWNEGAVFGAPVKDWKTIDLAAVHGVATINGSKVGEGRGADTMGHPLNAVVWLADHLA
                     SIGRGLLRGDVVITGSIITTKNVEPRDFVRFEVDELGSVELRVD"
     gene            complement(2898..3611)
                     /locus_tag="VFX94_10970"
     CDS             complement(2898..3611)
                     /locus_tag="VFX94_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5768747.1"
                     /translation="MSAAIVTGGSAGIGRAIVNELLKTNVEVISLDLQPSPDRHERLE
                     QLNVDLTDPAATRRVADEVSRAHQVTTLVHNAGVIRPALLPDVTLEDLDALVNLHLKT
                     ALILLQASLPAMKGAGYGRVVLVSSRAVLGLPTRTSYAATKAGMLGMARTWALELAPQ
                     GITVNVVAPGPIETDNFYSVVPRGQGERVAQSIPVRRLGQPGDVARAVMFFAHRDAGF
                     VTGQVLYVCGGASVGSLVL"
     gene            complement(3608..4105)
                     /locus_tag="VFX94_10975"
     CDS             complement(3608..4105)
                     /locus_tag="VFX94_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEX5768748.1"
                     /translation="MEAVHGRLMREDDPVRFVLREARLIDEKRFDEWYELFTDDAFYW
                     VPLAPGQTDPLAHNSLAYEDKLLLKLRIERLKLPTAYSQKPASRCLHVLQLPEVERSD
                     DARGEVFMRTPFLYTETRGDESQRYAALAWHTLVRTDAGLRIRLKRVDILNADAALPS
                     IQLFL"
     gene            complement(4081..5382)
                     /locus_tag="VFX94_10980"
     CDS             complement(4081..5382)
                     /locus_tag="VFX94_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX5768749.1"
                     /translation="MSRYRHDARALQGLVEDDRVHRDVYVDPEVFQLEMERLWSRTWI
                     YVGHTSQVRQPGDFITVDIAAKPLILVHHTDGGVRVLMNRCAHKGTKLVYDFSGNTGK
                     TFRCPYHAWTYRTDGTLLNVPLKGGYDGTRLYETSSARGLTPVTHETYRGFVFARLAD
                     GPGLRDYFGDSLSSIDNLADRSPEGELEITGGCLRYLHNCNWKMFVENLNDTMHPMIA
                     HASSAGTARRLWEGKPAELPKPMAIEQYAPFANDYKFFDDMGVQVYPHGHGFSGVNFS
                     IHSAYSAIPEYEEAMKRAYGEERAQRILGTVRHNTVYYPSLTIKGAIQSIRVARPLAA
                     DKTVIESWTLRLKGAPSKLLERTIMYNRLINSPMSVVGHDDLHCYRSAQEGLAAEGNE
                     WVSLHRNFDDRELSEKELTCNGTSEISMRNQFRAWKQFMAA"
BASE COUNT          929 a         1779 c         1941 g          935 t
ORIGIN      
        1 cgataatcgc gggcgccgag gcgcgggcgc gcggaaaaag cttctggtag gtgtcgaaca
       61 gcggctcgcg ccacacggcg atgcccggca ggtcgagcgc aaggacgagc gtgcagaccg
      121 aaagaaccgc cgtgcccagc gcgcgcgggc ccgcgccgat cctcagagct cgatctccag
      181 gccgtcgtag gcggcgatga cctccagccg ctgcacgccg cgcgtgagct cctccaggcg
      241 acgggtctcg cgcaggatgc gctcgatggc gtcgtcgccg ttggccggct cgtggtggaa
      301 gagcgccagc ctgcgcgcgt ttgccatctg gcagagttcc actccgacga cgttgctcga
      361 gtggccccag tcggctttca ccgatacggc ctcggcaagg gagtacattg cgtcgaagat
      421 gacgaggtcg gcgccgcgga agaactccac gatgcgcttg cgggcggcgt ggtcgtcgag
      481 cttgtgctcc gagtcggtgg tatagacgac cgacttgccg cggctcttga agcgaaagcc
      541 gtacgaatcc ccggagtggt gctgtagctg cggcgtaacc gtgaccgcgg gcagctcgtg
      601 cgcctgctcc ggggcgaggt ggatgaagtc gatgtccgcc gcgagctggg agaagggcac
      661 cggaaacgac ggcgggtcct gctggcggcg aaacgcgtgc tcgagcgcgt cgtggcagcc
      721 gtggatgcgg atattgttgc cgcgcacgta agccgggcca aagaagggaa agcccatgat
      781 gtggtcccag tgcatgtgcg acatgaacac gtagaccgtg gccgctcggc cgccctgacg
      841 cgcggcaatg tgctcgccga acgcccgcgc gccgcttccc atgtcgcaca cgaaatactc
      901 gtcgggaccc gcgtcgggcg gcatgagctc gacgcacgcg gaatgcccac cgaaggtgtg
      961 cgtcagggag aagcccagat gctgcccggc gaaggcgtga gctttttcaa gcgtatcgac
     1021 attcttgccg ttggccgcga ccagggcgcg cgcgaggcga tcgcgcacgt cgcgccaggt
     1081 gagcgccacc ggaagcgagc cgcgcgtgcc ccagaagcgc acccgcatcg gcgcgctacc
     1141 cgccgtcgaa tgcggccgcc gcctggggcg aggccgcggc gagcgcttcg cgcacgccgg
     1201 gataaatttc gaacagcatc gtgaagcggc tgatctggaa gatctcggca accagcgggc
     1261 gcatcgccgc gatcatgatg cgcccgccct gggtgcgcgc ctgcttgccg ccgagcatga
     1321 gaagccgcag tcccgccgag gacacgtact cgagcgccga gagatccagc acgagcgccc
     1381 gaccgtcgcg cgtctcgtcc acgagcggct ggagcgtggc cccgaaatcg tcgcagctgt
     1441 cgtggtccag gcgcccggcc ggggtaagca ccaggctgtc cgcgtagcgg cgctggatca
     1501 gcttcatcgc ccgttattct agaatgcggc gtggcgctcg cagcgttcaa tttcgcccgc
     1561 tggatcgacg agcatgcgca cctgctcagg ccgccggtcg gcaaccagat ggtgttccgc
     1621 gaggccgagg acctcatcgt gatggtgatc ggcggaccga atgcgcgcag cgactaccac
     1681 gacgatccct acgaagagtt cttctaccag ttgcgcggcg acatggtgct caaggtgatc
     1741 gagaacgggc ggccgcgcga cgtgccgatc cgccagggcg aaatcctgct cgtgccgggc
     1801 cattgccggc attccccgca gcgccctgcc gggacggtcg gcctggtagt cgagaaagtc
     1861 aggccgcgcg aggtgaagga cggcttcgaa tggtactgcc ccaggtgctg gggcctggtg
     1921 caccgggtgg acgtcaacgt tcagaacatc gttacggatt tgccgccgct tttcgaggcg
     1981 ttctacaaga gcgagcgcaa ctgtccgcgg tgcggcgccg tgcacccggg caagggctga
     2041 tggacgcgga agccgccgca cgctgcctgt tcgcgtcgca cgaacgccgc gagcgcttca
     2101 cgccgcttcc ggcccatctt gccccgcgca gcgttgcgca ggcacacgcc atccaggacg
     2161 ccttcgtcgc ccagcgggcg cagaagctcg gcggcatcgc ggggtacaag gtcgcgctca
     2221 cctcgcccca aatgcggcgc ttcgtcggcg tggacgagcc gcaggcgggc atgatgctcg
     2281 agaccacgct gcaccgatca cccgcgcgcg tgcgcgccgc tgacttcgtc cgtctggtcg
     2341 tggagttcga aatcgccgtg cagatcggcg atgacctccc agcggcggac agcccgttct
     2401 cgcgccagcg cgtggccgag gccgtggagg ccgtcatgcc ggcgctcgag ctcgccgacg
     2461 accgtaatgc cgactacagc gagctcgccc gccatccgct cgagctcatc gccgacaact
     2521 gctggaacga gggcgcggtt ttcggcgcgc cggtcaagga ttggaagacc attgatctcg
     2581 cggcggtgca cggcgtcgca accatcaacg gaagcaaggt cggggaaggg cgcggtgccg
     2641 acacgatggg ccatccgctc aacgctgtgg tgtggcttgc cgaccatctt gcctccattg
     2701 ggcgcgggtt gctgcgtggc gacgtggtca ttaccggcag catcattacg acgaaaaacg
     2761 tggaaccgcg cgacttcgtg cgcttcgaag tggatgagct cggctcggtc gagctccggg
     2821 tggactgacc tggacggtca gcggcgcgag cggcaacgac agccaggtgg cacgagctcg
     2881 aacgacaggt ggcgcggcta aaggacgaga ctgccgacgc ttgctccgcc gcatacgtac
     2941 agcacctggc cggtgacgaa gcccgcgtcc cggtgggcga agaacatcac tgcgcgcgcc
     3001 acgtcgccgg gctggccgag gcgcctgacc gggatggatt gtgcaacgcg ctcgccctgg
     3061 ccgcgaggca ctacgctgta gaagttgtcg gtctcgatcg ggccgggcgc caccacattc
     3121 accgtgatgc cttgcggcgc cagctcgagc gcccaggtgc gcgccatgcc gagcatgccg
     3181 gccttggtcg cggcgtacga cgtgcgagtc ggcagcccga gcacggcgcg tgatgagaca
     3241 aggaccacac gcccgtagcc ggcgcccttc atggcgggca gcgaagcctg cagcaggatg
     3301 agggccgtct tgagatgaag gttgacgagg gcgtccaggt cttcgagcgt cacgtcggga
     3361 aggagggcag ggcggatcac cccggcgttg tggacgagcg tcgtaacctg gtgcgcacgc
     3421 gaaacctcgt ccgccacgcg ccgcgtggcc gcggggtcgg tcagatccac gttcagctgc
     3481 tcgagcctct cgtggcggtc gggcgacggc tggaggtcga gcgagatcac ttcgacattc
     3541 gtcttgagca gctcgttgac gatcgcccgg ccgatccccg cgctgcctcc ggtgacgatc
     3601 gcggcgctca caggaagagc tggatggagg gcagggcggc gtcggcgttc aggatgtcga
     3661 cgcgcttaag tcggatgcgc agccccgcat cggtgcgcac gagcgtgtgc caggcgagcg
     3721 cggcgtagcg ctgcgactcg tcgccccgtg tttcggtata gaggaatggc gtgcgcatga
     3781 aaacctcgcc gcgcgcatcg tccgagcgct cgacctcggg aagctgcagg acgtgcaggc
     3841 agcggctcgc cggcttctgc gagtacgcgg tcggcagctt caggcgctcg atgcgcagct
     3901 tgaggagcag cttgtcctcg tacgcgagcg agttgtgtgc gagcggatcg gtttgcccgg
     3961 gcgcgagcgg aacccagtag aaggcgtcat cggtaaaaag ttcgtaccat tcgtcgaagc
     4021 gcttttcgtc gatgagcctc gcctcccgca gcacgaagcg taccggatcg tcctcccgca
     4081 tcaggcggcc atgaactgct tccatgcgcg gaactggttg cgcatggaga tctcgctcgt
     4141 gccattgcag gtcagctctt tctcgctcag ctcccgatcg tcgaagttgc ggtgcaggct
     4201 tacccattcg ttgccctccg cggcgaggcc ttcctgcgcc gagcggtagc aatgcagatc
     4261 gtcgtgcccg acgaccgaca tgggcgagtt gatcaggcgg ttgtacatga tcgtgcgctc
     4321 gagcagcttc gacggcgcgc ccttcaggcg caaggtccag gactcgatca ccgtcttgtc
     4381 ggcggcgagc ggccgcgcca cgcggatcga ctggatcgcg cccttgatcg tgaggctggg
     4441 gtagtagacg gtgttgtggc gaacggtgcc caggatccgt tgtgcgcgct cttcgccata
     4501 agcgcgtttc atcgcttcct cgtactccgg gatcgcggag taggccgagt gaatcgagaa
     4561 attgacccct gaaaagccgt ggccgtgcgg atacacctgc acgcccatgt cgtcgaagaa
     4621 cttgtagtcg ttcgcgaatg gtgcgtactg ctcgatcgcc atgggcttgg gcagctcggc
     4681 gggcttgccc tcccacaagc gcctcgccgt gccggcggag gaggcgtgcg cgatcatcgg
     4741 atgcatcgtg tcgttcagat tctccacgaa catcttccag ttgcagttgt ggaggtatct
     4801 caggcagccg ccggtgatct cgagctcgcc ctcgggcgag cggtcggcaa ggttgtcgat
     4861 cgaggaaagc gagtcgccga agtagtcgcg caacccgggg ccgtcggcga gccgcgcgaa
     4921 cacgaagccg cgatacgtct cgtgcgtcac gggcgtcaga cctcgcgcgc tggaggtctc
     4981 gtacagcctc gtgccgtcat aaccgccctt cagcggcacg ttgaggaggg taccgtccgt
     5041 acggtaagtc caggcgtggt acgggcagcg gaaagtcttg ccggtgttgc cggagaagtc
     5101 gtagacgagc ttggttcctt tgtgcgcgca gcggttcatc agcacgcgca cgccgccgtc
     5161 ggtatggtgg acgaggatga gcggctttgc cgcgatatcg accgtgatga aatcgcccgg
     5221 ttgccgcacc tggctggtgt gcccgacata gatccaggtg cgtgaccaaa gccgctccat
     5281 ttccagctgg aacacctccg ggtcgacata gacatcgcgg tgcacgcggt cgtcctcgac
     5341 aaggccctgc agggcgcgcg cgtcgtgccg gtaacggctc atggcggcat tttaggagca
     5401 gcttcgcgtc ggcgccgccc gaccgccatt tcctcgaata aacaaggcgg gcggcgacat
     5461 atctgccttc ggatgtcgct cccgcgccac ggcgtgcggg tgatctgagt tgccgcgcgt
     5521 tgtactaagt ctctgattgg acatagggcg gctgattggc acaggtcgtg cgttcccgct
     5581 acag
//
LOCUS       DASUNT010000542         2336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_773029, whole genome shotgun sequence.
ACCESSION   DASUNT010000542 DASUNT010000000
VERSION     DASUNT010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773029"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..246)
                     /locus_tag="VFX94_10985"
                     /pseudo
     CDS             complement(1..246)
                     /locus_tag="VFX94_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364372.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
     gene            complement(258..1247)
                     /locus_tag="VFX94_10990"
     CDS             complement(258..1247)
                     /locus_tag="VFX94_10990"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX5768750.1"
                     /translation="MTWYGLARPFLFSLDPEEAHRLALRLAGLVIPKRFDSPVRAMGL
                     EFPNPVGLAAGLDKDAAHIDALARMGFGFLEVGTVTPRPQPGNPRPRLFRIPEKQALI
                     NRLGFNNIGVDAFLQNVARARYRGILGINIGRNAVTPPERAVDDYELCLEKVYAAASY
                     VTINVSSPNTAGLRSLQEKNSLEALLQRLAARRQELAARHRKHVPLALKIAPDLETDD
                     IRAISEAVRRYGIDAVIATNTSVSRDGVESLPHAEEAGGLSGAPILARSTAVLRELKT
                     LLPDITLIGAGGILSGADAAQKRAAGARLVQLYTGLIYRGPDLVGECVGYLSP"
     gene            complement(1261..2022)
                     /locus_tag="VFX94_10995"
     CDS             complement(1261..2022)
                     /locus_tag="VFX94_10995"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEX5768751.1"
                     /translation="MSRLNDLPHAVLQFYVTAPYPCSYLPDRMARSQVATPSHLINTE
                     LYGDLVKTGFRRSGIFTYRPHCDACRACVPVRIPVAHFSANRSQRRAWKQHCSLTAHP
                     QPLKFRLEHYALYLRYQSKRHSGGGMDNDSRDQYSHFLLQSRVDTRLVEFRDSGQLVM
                     VSIVDYLPDGLSSVYTFYEPDRRNASFGTYNVLWQIEQCRSLAQPYLYLGYWIRESPK
                     MAYKSKFEPIEGFVGGAWRRLGRRSAEENRDEWRS"
     gene            complement(2009..>2336)
                     /locus_tag="VFX94_11000"
     CDS             complement(2009..>2336)
                     /locus_tag="VFX94_11000"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX5768752.1"
                     /translation="RLHREGHAHSFETWLGDRLVGGLYGVAIGRMFFGESMFSRVSDA
                     SKVALATLVGELRAREFPLIDCQMHTPLLASLGAREIPRRTFLRELTALVNYPQSSGK
                     WSRVTT"
BASE COUNT          393 a          757 c          814 g          372 t
ORIGIN      
        1 cagccgccgc acgcgggcgc gtccccgcgc accggcagcg cgccggagct gcgcgttcca
       61 ttcgccctga gcgggcacca tggaaatgca atcgatcggg cagggcggca cgcagagctt
      121 gcagccgatg caccagggtt ccaccacggt gtgcatgagc cgcgccgcgc cgacgatggc
      181 gtcgaccggg caggcgtcca tgcacagcgt gcagccgatg cagcggctct cgtcgatcag
      241 ggcgacggcg gtgcgcttca tgggctcaag tatccgacac attcacccac cagatccggc
      301 ccgcggtaga tgaggccggt gtagagctgg accagccgcg ctccggcggc ccgtttctgc
      361 gccgcgtccg cgcccgaaag gatcccgccc gccccgatga gcgtaatgtc cggcagaagc
      421 gtcttcagct cgcgcagcac cgcggtggat ctcgcaagaa tcggggcgcc cgagaggcca
      481 ccggcctctt ctgcatgcgg caggctctcc acgccgtcgc gcgagacgga ggtattcgtc
      541 gcgatcacgg catcgatgcc ataccggcgc accgcttccg agatcgcccg gatgtcatcc
      601 gtttccaggt caggcgcgat cttgagcgca agcggcacgt gcttgcggtg gcgcgcggcg
      661 agctcctgcc tgcgggcggc cagcctctgc agaagggctt ccagggaatt cttctcctgc
      721 agcgagcgca agccggcagt gttcggggag gagacgttga tggtgacgta ggaggcggcg
      781 gcgtagacct tctcgaggca cagctcgtaa tcgtccaccg cgcgctcggg cggcgtgacg
      841 gcgttgcggc cgatgttgat gccgaggatg ccgcggtagc gcgcccgcgc cacgttctgc
      901 agaaatgcat cgacgccgat gttgttgaag cccaggcggt tgatgagagc ctgcttttcg
      961 ggaattctga acaaccgggg acgcggattg cccggctggg ggcgcggggt gacggttccg
     1021 acctcgagga agccgaaccc catgcgcgcg agcgcgtcga tgtgcgccgc gtccttgtcc
     1081 agccccgccg ccaggccgac cgggttcgga aactccaggc ccatcgcccg gaccggggaa
     1141 tcgaaccgct tggggatcac cagccccgcg agtctcagcg cgaggcggtg tgcttcttcg
     1201 ggatctagcg agaaaagaaa aggtcgggcg agcccatacc acgtcacgtc cgcttcccgc
     1261 tcacgagcgc cattcgtccc tgttctcttc ggcgctcctg cggcccaggc gccgccacgc
     1321 tccgccgacg aagccctcga tcggctcgaa cttggacttg tacgccatct tagggctctc
     1381 ccggatccag tagccgaggt agagataggg ctgcgcgagc gagcggcact gctcgatctg
     1441 ccaaagcacg ttataggtgc cgaagctcgc gttgcgccgg tcgggctcgt agaacgtgta
     1501 aaccgaggac aggccgtcgg gcaggtagtc gacgatagac accatgacga gctggcccga
     1561 gtcccggaat tcgaccagcc gggtgtcgac ccggctctgc agcaggaagt gcgagtactg
     1621 gtcgcggctg tcgttgtcca tcccgccgcc ggaatggcgc ttggactggt agcgcaggta
     1681 gagcgcgtag tgctcgaggc ggaacttgag cggctgcgga tgcgcagtga gactgcagtg
     1741 ctgcttccac gcccgccttt gcgagcggtt ggcgctgaag tgggcgaccg ggatgcgaac
     1801 cggaacgcag gcgcggcatg cgtcgcaatg cggccggtag gtgaagatgc cgctgcggcg
     1861 gaagccggtt ttgacgagat cgccgtacag ctcggtgttg atgaggtggc tcggcgtcgc
     1921 cacctgcgag cgcgccatgc ggtccggcag gtagctgcac ggatagggcg cggtcacgta
     1981 gaactgcagt accgcgtgcg gcaggtcatt aagtcgtgac acggctccac tttccagacg
     2041 actgcgggta gtttaccagc gccgtcaatt cgcgcaggaa ggtgcggcgc gggatctcgc
     2101 gggcgccgag ggaagcgagc agcggcgtgt gcatctggca atcgatcaga gggaactcgc
     2161 gcgcccggag ttccccgacc agcgtcgcca gcgccacctt cgaggcgtcg ctcacgcgcg
     2221 agaacatcga ctcgccgaag aacatccggc cgatggccac gccgtagagc ccacccacga
     2281 ggcggtcccc gagccaggtc tcgaagctgt gcgcatggcc ttcccggtgc aggcgc
//
LOCUS       DASUNT010000543         2136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_773105, whole genome shotgun sequence.
ACCESSION   DASUNT010000543 DASUNT010000000
VERSION     DASUNT010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773105"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..577
                     /gene="gspG"
                     /locus_tag="VFX94_11005"
     CDS             176..577
                     /gene="gspG"
                     /locus_tag="VFX94_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEX5768753.1"
                     /translation="MPNRNSGFTLLELLVVIVIIGLLAGYVAPRYFSQVGKSEIQVAR
                     AQIDALEKALDQFRLDHRRYPSAEEGLAALEPYLKKALPNDPWGRPYVYRTPGQRGAY
                     EVISYGRDGKAGGSGEDADVGIASREVSLSK"
     gene            936..1364
                     /locus_tag="VFX94_11010"
     CDS             936..1364
                     /locus_tag="VFX94_11010"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX5768754.1"
                     /translation="MPTHTERRDGDRLERGTAYVALPNRHLLVNPDGTLSLSQSELVH
                     FVRPSADLLFESAAASYRERAIAVVLSGSGRDGAMGVKAIKKTGTVIVQDERTRRGNT
                     AAAEKCFDKARELHERIAAVREAALGSEELDMEKLREKAG"
     gene            complement(1377..2057)
                     /locus_tag="VFX94_11015"
     CDS             complement(1377..2057)
                     /locus_tag="VFX94_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768755.1"
                     /translation="MGRKLKTQQLTDSILGQHLALSVASHLARTQLVPEPLAVCDSQH
                     LGDMVDIVARALAKVAPLYVRDAGSGQPRELAAGELEGAVISRAATLLALMDGRTFSS
                     VSIKRADLRQAIAVLKAVGIEELAPRRKPESPPRSEARERVAELRTRIAEIEALLRPP
                     LLAEQVERANRLIVSTARQAPDGRVANLAMRLMTVVQEAKDSDEPSQSVQAALVRLRT
                     ALEERQGG"
BASE COUNT          336 a          725 c          746 g          329 t
ORIGIN      
        1 aaagcaccgg tcgctttggc gatcttttcc gtattgcagc gagcgggtcc atgaacggca
       61 ccgcgcgcaa ggtgctgttg cgctcgccca cgatcgtgtc agacccgagc ggccaaacct
      121 gcagcctcga gcagatccgg gcgctgctgt actcgggata gtgaaaggaa aacgcatgcc
      181 caaccggaat tccggcttca cgctgctcga gctgctcgtg gtaatcgtga tcatcggcct
      241 gctcgcgggc tacgtcgcgc cgcgctattt ctcgcaggtc gggaagtcgg agatccaagt
      301 ggcgcgcgcg caaatcgacg cgctcgaaaa agcgctcgac cagttccggc tcgaccaccg
      361 ccgctacccc tccgcggagg agggcctggc ggcgctcgag ccttacctga agaaggcgct
      421 gccgaacgac ccctggggcc ggccgtacgt ataccgaacg cccggccagc gcggcgcgta
      481 cgaggtgatc tcctacggcc gcgacggcaa ggcgggcggc agcggcgagg acgcggacgt
      541 cgggatcgcg agccgggaag tctccctcag caagtagcga cgcgcgctcg gcgatgcgat
      601 tcaaactgct cgccgtagat gccggcgacg ctgcgcgaat cccagacgcg gacgaagtcg
      661 atcacggtct gccttcgccg atgcgccgcg gcacctcggc gaactcgggc gtaccgcagg
      721 tcgtcggtgc cggccttgaa gctcaagccc aggatcccga cctcgcgctg gcctttctcc
      781 accaccgctc accatttcgc gcatcgtccc cggcagcatg gtgctgcgca tcacgatgac
      841 aggggcgcca gggtgcgtgc ggatcgcctc cccgatctgc tcgcagaccc gctttacgta
      901 gcgcaggtcg attgccgccg ttcggcaggc taggcgtgcc gacgcacacg gagcggcgcg
      961 acggtgacag gctggagcgc ggcacggcgt acgtcgcgct gccgaaccgg cacttgctgg
     1021 tgaacccgga cggcacgctg tcgctctctc agtcggagct ggtccacttc gtgcggccct
     1081 cggcggacct gctttttgaa tcggccgccg cgagttaccg tgagcgcgcc atcgccgtgg
     1141 tactgtcggg ctcggggcga gacggcgcca tgggcgtgaa ggcgatcaag aagaccggca
     1201 cggttatcgt gcaggatgag aggactcgcc gcggcaacac cgccgcggca gagaagtgct
     1261 tcgacaaggc gcgcgagctt cacgagcgca tcgcggcggt gcgcgaagcg gcgctcgggt
     1321 ccgaagagct cgacatggag aagctgcgcg agaaggcggg ctaggtccgc gccttttcag
     1381 ccgccctggc gttcctcgag cgcggtgcgc agccgcacca gcgcggcctg cacgctctgc
     1441 gagggctcat cgctgtcctt ggcctcctgc accaccgtca tcagccgcat cgcgaggttg
     1501 gcgacgcggc cgtcgggcgc ctggcgcgcg gtgctgacga tgaggcgatt ggcgcgctca
     1561 acctgctcgg caagcagcgg cggccgcagc agggcttcga tttcggcgat gcgggtgcgc
     1621 agctcggcca cgcgctcgcg cgcctcgctc cgcggcgggc tctcgggctt gcggcgcggc
     1681 gcgagctcct cgatgccaac ggccttcagc acagcgatcg cctggcgcag gtcggcgcgc
     1741 ttgatcgaaa cgctcgagaa ggtgcggccg tccattagcg cgaggagcgt cgcggcgcgg
     1801 gagattaccg cgccttcgag ctcgcccgcc gccagctcgc gcggctgccc tgagcccgca
     1861 tcgcgtacat agagcggcgc gaccttggcg agcgcgcgcg ccacgatgtc caccatgtcg
     1921 cccaggtgct gggagtcgca gaccgcgagc ggctcgggca cgagctgcgt gcgcgccagg
     1981 tgcgaggcta ccgacaaggc gaggtgttgc ccgaggatgc tgtcggtgag ctgctgcgtc
     2041 ttcaactttc ggcccacggg gcctatttta tgaaaatcgc ctcgctgcgc agccgggaaa
     2101 gccccgacag ccgggcccgc catatggtgc aggcgg
//
LOCUS       DASUNT010000544         4057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_685695, whole genome shotgun sequence.
ACCESSION   DASUNT010000544 DASUNT010000000
VERSION     DASUNT010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685695"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..700)
                     /gene="nthA"
                     /locus_tag="VFX94_11020"
     CDS             complement(101..700)
                     /gene="nthA"
                     /locus_tag="VFX94_11020"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEX5768756.1"
                     /translation="MKDQGKYRPLSYFQLMEISLRELLVEKGILTNEQVDAEVEDMRR
                     RTPERGAKVVARAWVDSDFRKRLLENGTKACEELGLDIPALKLVVVENTPEVHNAIVC
                     TLCSCYPRMLLGIPPDWYKSRNYRSRMVREPRKVLSEFGLVLSEKIQVRVHDSSADMR
                     YMVLPMRPAGTEGWSEERLAALVNRDSMIGVAIPKGPNG"
     gene            complement(697..987)
                     /locus_tag="VFX94_11025"
     CDS             complement(697..987)
                     /locus_tag="VFX94_11025"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HEX5768757.1"
                     /translation="MPAKNRFKPGDRVRVLKAYPPGHIRTPYYIRGCTGEIERICGTF
                     PNPEELALMRSGLPAQPLYRVRFLQKDVWPDYSGNARDAVEVEIFQHWLDPA"
     gene            complement(971..1588)
                     /locus_tag="VFX94_11030"
     CDS             complement(971..1588)
                     /locus_tag="VFX94_11030"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768758.1"
                     /translation="MIERSHHDMGGQPAGEVQPSEHDYADWERRIDAMAVLLWGIKGT
                     KKLFSVDEHRRAIESLPPQAYDSMTYYEKWIYALAQCLIARGVITSEELARKMAGVGG
                     GHAGPRSHHDMGGLGAGKVIPSEHDYEQWERRIDALSVILGRNVRITVDERRKAIESL
                     PPDAYDTMSYYERWTAALAHTMLQRGMITTEELARKMAEVDARKK"
     gene            complement(1590..3284)
                     /locus_tag="VFX94_11035"
     CDS             complement(1590..3284)
                     /locus_tag="VFX94_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5768759.1"
                     /translation="MNTPVELATLTGAEAVVEMLRAHGVDTMFGLCGDTSLPLYDALA
                     RLGSIRHILTRDERHAAYMADGYARISGRVGVCEGPSGGGATYILPGLVEANESSIPV
                     LAVNTDVSVSSRGKFTLTELDQRALMKPLTKWNAVLDRSADIPRVLRKAFDAMTTGRP
                     GAAHIALPFDVQNGPVDRADIWADPTLGSFPSRRVAPDPALVELAAKLLRNAKQPLFV
                     CGGGVVLSGAEGELSTLAETISAPVATTISGKGSIDERCAHSAGVVGSNGGTPETRAV
                     VDAADVIVFVGCRAGSVTTERWRHPQAGKARIIHIDVDPAVPGTNYKVDVPLVGDARL
                     CLSALIDALEGHKREADLTWVDAKKREKFEKFEALARSDEQPIKPERVVFELQTHLDR
                     DAILVADPGTPCPYFSAYYVVKGTGRRYFSNRAHGALGYAMAAAMGAHVARPRVKTVA
                     VMGDGSFGMCVGELETAVRLKLPVTFIVISNAVYGWIKAGQKSGYERRYFSVDFGATD
                     HAKVAEAFGVKSWRVFDPARLGTALKSALAHDGPSLVDIVCQPLHEARAPVSEWVA"
     gene            3471..>4057
                     /locus_tag="VFX94_11040"
     CDS             3471..>4057
                     /locus_tag="VFX94_11040"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5768760.1"
                     /translation="MDVAERQRPPPGELCLDHLAHFVLDLDAAAAVWEKLGLRVTPVS
                     VHQVSGKPAGTSNRCVMFEEGYVELLAPTQKTAHAGRVHSLMKRYDGVHLACFGTPSA
                     AAEYARLAAHGFEPEPVVKLERKLKTGEAVRFNVVYVPPGKMAEGRVQYCEHLTPQHV
                     WRRGFVNAFRLRSVYVVADDPEEVAARWGRFGGLLP"
BASE COUNT          671 a         1343 c         1303 g          740 t
ORIGIN      
        1 cgctgttcac ctcgaggtct agcgtcgtgc agaactccac atacgccgcc gagtcgccga
       61 gcggcactac gcctaaaggc cgcgccttag ccaagcagtc ctagccgttg gggcccttag
      121 ggattgcaac gccgatcatg ctgtcccggt tgacgagcgc ggcgaggcgc tcctcgctcc
      181 agccctcggt gccggcgggc cgcataggga ggaccatgta gcgcatgtcg gccgaggagt
      241 cgtgaacgcg tacttgtatt ttttcggaca gcaccaaccc gaattccgaa agcaccttgc
      301 ggggctcgcg caccatgcgg gagcggtaat tgcgcgattt ataccagtcg ggcgggatgc
      361 cgaggagcat gcgcgggtag caggagcaca gcgtgcagac gatggcgttg tgaacttcgg
      421 gtgtgttttc gaccaccacc agctttagcg cggggatatc gagccccagc tcctcgcatg
      481 ccttggtccc gttctccagc aggcgctttc gaaaatcgga gtcaacccag gcgcgcgcca
      541 cgaccttggc gccgcgctcg ggcgtgcgcc ggcgcatgtc ctccacttcg gcgtcgacct
      601 gctcgttcgt gaggatgcct ttctccacca gcagctcgcg caggctgatt tccatcagct
      661 ggaaatacga gagcggccgg tatttgccct ggtctttcat gcggggtcca gccagtgttg
      721 aaagatttcg acctccaccg cgtcgcgcgc gttgcccgaa tagtccggcc agacgtcttt
      781 ctgcagaaac cggactctgt aaagcggttg agcgggaagc ccggagcgca tgagcgcgag
      841 ctcctcggga ttggggaacg tgccgcatat gcgctcgatc tcgccggtgc acccgcggat
      901 gtagtacggc gtgcggatat ggccgggggg ataggccttc agcactctta cccggtcacc
      961 gggcttgaat ctattttttg cgggcatcga cttccgccat cttgcgggca agctcctcgg
     1021 tggtaatcat cccgcgctgg agcatggtgt gcgcaagcgc cgcggtccag cgctcgtaat
     1081 agctcatcgt gtcatacgca tccgggggca gggactcgat ggctttgcgg cgctcgtcca
     1141 ccgtgattct cacgttcctt ccgagaatga cggaaagggc atcgatccgg cgctcccatt
     1201 gctcgtagtc gtgctcgctc ggaattactt tccccgcccc aaggccgccc atgtcgtggt
     1261 gactccgagg gcccgcatgt cctcctccca cgccggccat cttgcgcgcg agctcctcgc
     1321 ttgtgatcac gccgcgcgcg atgaggcact gcgcgagcgc gtagatccat ttctcgtagt
     1381 aggtcatgga gtcgtacgcc tgcggcggca gcgattcgat cgcccggcgg tgctcgtcca
     1441 ccgagaagag cttcttcgtt cccttgattc cccacagcaa taccgccatc gcgtcgatgc
     1501 gccgctccca gtcggcatag tcgtgctcgc tcggctgcac ctcgccagcc ggctgcccgc
     1561 ccatgtcgtg gtggctgcgc tcaatcatgt catgcgaccc attccgatac cggcgccctc
     1621 gcttcgtgca gcggctggca gacgatgtcc accagagagg ggccgtcgtg cgcgagcgca
     1681 cttttcaggg ccgtgccgag tcttgcggga tcgaagaccc gccagctctt cacgccgaaa
     1741 gcctcggcca ccttggcgtg atcggtcgcg ccgaaatcca cggaaaaata gcggcgctcg
     1801 tagcccgatt tctggccggc cttgatccag ccatagaccg cgttggagat gacgatgaaa
     1861 gtgacgggca gcttgagccg caccgcggtc tcgagctccc cgacgcacat gccgaagctg
     1921 ccgtcgccca tgacggcgac ggtcttcact cgcggccgcg cgacgtgcgc gcccatcgcc
     1981 gcggccatgg cgtagccgag cgccccgtgc gcgcggttgg aaaagtaccg ccgcccggtg
     2041 cccttcacga catagtaggc ggaaaagtac gggcacggtg tccccggatc ggcgaccaag
     2101 atcgcatcgc gatcaagatg ggtctgcagc tcgaagacaa cccgctccgg cttgatcggc
     2161 tgctcgtcgg agcgcgccag cgcttcgaac ttctcgaatt tctcacgctt tttcgcgtcg
     2221 acccacgtga ggtccgcttc tcgcttatgg ccctccaatg cgtcgatcaa tgccgagaga
     2281 cagaggcgag cgtcgccgac gagcggcaca tcgaccttgt agttggtccc cggaaccgcg
     2341 gggtcgacgt cgatgtggat gatcctcgcc tttccggcct gcggatggcg ccagcgctcg
     2401 gtggtcaccg agccggcgcg gcagccgacg aagacgatca cgtcggcggc atcgaccacc
     2461 gcgcgcgttt ccggcgtgcc gccgttcgag ccgacgactc cggcagaatg ggcgcagcgc
     2521 tcgtcgatcg agcccttgcc gctgatcgtc gtagcgaccg gagcggagat ggtctccgcc
     2581 agagtgctca attccccttc ggcaccggag agcaccacgc ccccaccgca gacgaagagc
     2641 ggctgcttcg cgttgcgcag cagcttcgca gcaagctcca ccagcgcggg atcgggcgcg
     2701 acgcgccgcg acggaaaaga accgagcgtc ggatcggccc atatgtcggc gcggtccact
     2761 ggcccgttct gtacatcgaa cggtagcgcg atgtgcgcag caccggggcg ccccgtggtc
     2821 atggcgtcga acgccttgcg caacacccgc gggatgtccg ccgagcgatc gagcaccgcg
     2881 ttccatttcg tcagcggctt catcaaggcg cgctgatcta gctccgtgag cgtgaacttg
     2941 ccgcgtgaag agacggagac atccgtattg acggcgagca ccggaatcga ggattcgtta
     3001 gcctctacga gcccgggcag gatgtaggtg gcgccgccgc ccgaagggcc ttcgcatacg
     3061 cccactctgc cgctgatgcg cgcatagccg tctgccatgt acgcagcgtg gcgctcgtcg
     3121 cgcgtgagga tgtgcctgat ggagccgagg cgggcgagcg cgtcgtagag cggcaggctg
     3181 gtatcgccgc acagcccgaa catcgtgtcc acgccgtggg cgcgcagcat ctcgaccacc
     3241 gcttccgccc cagtcagggt cgcgagctct acgggcgtgt tcacgccctt atattagggc
     3301 gggctggccg atttcaccac gaggctcgag ccagtagcgc tcgccccggt ggtcgtacca
     3361 gcgtttggtc tcgagatcca ggttcgcgca gaccgcgccg tcgccgtacg cgatgccgtc
     3421 ggcgcaggag aaatgccagc tttagcgtcg agtatgctat ctgcaccgcc atggacgtcg
     3481 ccgaacgcca gcgcccgcct ccgggcgagc tctgcctcga ccaccttgca cactttgtgc
     3541 tcgacctcga cgccgcggcc gccgtgtggg agaagctcgg cttgagggtc acgccggttt
     3601 cagtgcacca ggtctcgggg aaacccgccg gaacgtccaa ccgctgcgtc atgttcgagg
     3661 aaggctatgt cgagctcctt gcaccgactc agaaaaccgc gcacgcgggg cgggtgcact
     3721 cgctcatgaa gcgctacgac ggcgtacacc tcgcctgctt cggcacgccc agcgccgcgg
     3781 ccgagtacgc gcggctcgcc gcgcatggct tcgagcccga gccggtagtg aagctggaga
     3841 ggaaactcaa aacgggcgag gcggtgcgct tcaacgtcgt ctatgtgccg ccgggaaaga
     3901 tggccgaagg ccgagtgcag tattgcgagc atctcacccc tcagcacgtc tggcgccggg
     3961 gcttcgtcaa cgcgtttcgc ttgcgaagcg tttatgtcgt tgcggacgat ccggaggagg
     4021 ttgcggcgcg ttgggggcgc ttcggcggcc tgctgcc
//
LOCUS       DASUNT010000545         5705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_248184, whole genome shotgun sequence.
ACCESSION   DASUNT010000545 DASUNT010000000
VERSION     DASUNT010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248184"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VFX94_11045"
     CDS             complement(<1..628)
                     /locus_tag="VFX94_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5768761.1"
                     /translation="MRFVATLAALAFSTAALAQKETAIRFALDWRFEGPAAPYFVAID
                     KGYYKAEGLNVSIDPGAGSVEGINRVASGAYEMGFADINSLVKYRDNPRNLPVKAVMM
                     VYDTPAFSIVTLKKTGIAKPKDLEGKVLGAPAPDGAYAQWPIFVQANKIDASKVKIEN
                     IGFPVREPMLAQGKVDAITGFWFSSYMNLKANGVKDEDIVVLHMRDYGV"
     gene            676..2001
                     /gene="xdhA"
                     /locus_tag="VFX94_11050"
     CDS             676..2001
                     /gene="xdhA"
                     /locus_tag="VFX94_11050"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HEX5768762.1"
                     /translation="MRSSLEFFLNGERVSLPASRGTRTVLAWLREERRLTGTKEGCAE
                     GDCGACTVVVSEPGSDLPVCSCIYLLGALDGKALTTVEGLKGHPAQRAMVECHGSQCG
                     FCTPGFVMALYAHYRNPSGALADALAGNLCRCTGYAPILAAGKRMFEYAHATSEESHA
                     PQRVPAEGVQDRCFSAPRNLVELGRVLQGGATLLGGGTDLGLVVTKQLKDWEQVVYLG
                     NVAELKSIVDRGSFLEIGGAVTYSQAHPALAAIHPDIGELLRRLGGAQVRACGTIAGN
                     VANGSPIGDSMPFLLALGATVLLQKGEGQREVRLDTFYTGYRKSVLQPGEFIRLIRVP
                     RLAPGARFAAYKVSKRFDQDISAVCAAFHVEGAQARFGFGGMAATPSRAPRAEAAFAQ
                     GVEAACAALAHDFKPLSDHRASAWYRLTLAQNLLRKFARGASPTAIFKL"
     gene            1998..4211
                     /gene="xdhB"
                     /locus_tag="VFX94_11055"
     CDS             1998..4211
                     /gene="xdhB"
                     /locus_tag="VFX94_11055"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HEX5768763.1"
                     /translation="MIREPLPHDSASLHVSGEARYVDDLPEPPGTLQAAIGVSTETFA
                     KLLCLDLDPVRRAPEVVAVISAADIPGSNNIGPVLPDEPIFALDKVEFRGQPLFAVAA
                     TSVEAARRAARLARAEYQPHEPVVSVEQALERKQFVVPSIVVSRGTIRRDSPYSARGK
                     LRCGGQEHFYLEGQVALAIPGERGAMLVHSSTQHPTEVQHLVAVALGVPDARVTVELR
                     RLGGGFGGKETQAALYAVVAALLARATRRPVKLRADRDDDMIGTGKRHDFLYEYEAGF
                     DREGRIEALDLMLASRCGISADLSGAVNDRAVFHCDNAYFLPNVRITSHRCKTNTVSN
                     TAFRGFGGPQGMFAAEGVIAAIARRLGIDALDVRKRNLYGEAPRNITHYGMTVKDNVL
                     PELVEELETRSEYRRRRAAIADFNRTNEAYKKGLALTPVKFGIAFTTVSMNQAGALVH
                     IYTDGSVHLNHGGTEMGQGLMIKVAQLVAEELGVPLDRVQITPTSTAQVPNTSPTAAS
                     SGTDVNGEAARAACRVLKERLAPFREKFPEASFAELAREAFLARVPLSATGYWRTPDI
                     HFDRESFTGEPFYYFAYGAAVSEVAIDTLTGEWRLLRADLLHDVGRSLNPAIDRGQVE
                     GGFIQGMGWLTMEELVWDAGGALATHAPSTYKIPVAGDCPRQFNVWLWERGENLRDVA
                     YRSKAVGEPPLMLALSVFHAIYDAIGATGDSRDPVPLEAPATPENILRAICQLRS"
     gene            4193..4951
                     /gene="xdhC"
                     /locus_tag="VFX94_11060"
     CDS             4193..4951
                     /gene="xdhC"
                     /locus_tag="VFX94_11060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HEX5768764.1"
                     /translation="MPIEILIVETRGSAPREAGTRMWVDGGQIHGSIGGGNLEYTALK
                     IAREMLLSGEEFRQRKFSLGDSLGQCCGGSVTLHFTKKALFVFSETTCFHLALFGAGH
                     VGKEVARIVERLPCKVTWIDPRPDVFPSTVGGNVKVVIEEEPAWMVDEASANAYYLVM
                     THSHALDLEIVERVLERRDVAFLGLIGSQTKAAKFHLRLKAKGIDSSPLVCPVGLFKA
                     GKHPAEVAVSAVAQLLARRAQVPLGSALERVGGA"
     gene            complement(4889..>5705)
                     /locus_tag="VFX94_11065"
     CDS             complement(4889..>5705)
                     /locus_tag="VFX94_11065"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5768765.1"
                     /translation="FCGIAGIRPTHGLVSVENILPLCPSLDTVGPLTRTVEDAALVLE
                     VIAQADLTRELERPVSGLRIGRPRNVFYEGCEPEIAAAMEASLDVYRRLGATIVDIEL
                     ADFARLGALAAVLHAAEANPFHDEWFRTRPQDYSAHVRERLERGRPTLALDYVKALQA
                     RAPAIAAFCEAVFSKVDVVHGPVVSFQTPTIAQTDISTGEAAAQMLGSFVRLTRPISY
                     LGLPVVVANAGFTREGMPIGMQLIAPPHDDASALRAAHAFQRATEWHLRAPRE"
BASE COUNT          964 a         1894 c         1886 g          961 t
ORIGIN      
        1 cgacgccgta gtcgcgcatg tgcagcacaa cgatgtcttc gtccttcacg ccgttcgcct
       61 tcaggttcat gtaagaggag aaccagaacc cggtgattgc atcgaccttg ccctgcgcaa
      121 gcatcggctc gcgcaccggg aagccgatgt tctcgatctt caccttcgac gcgtcgatct
      181 tgttcgcctg cacgaagatc ggccactggg cgtacgcgcc gtcgggggcc ggcgcgccga
      241 gcaccttgcc ttcgaggtcc ttcggcttgg cgatgccagt cttcttcagc gtcacgatgg
      301 aaaaggccgg cgtgtcgtac accatcatca ccgccttcac cggcaggttg cgcgggttgt
      361 cgcgatactt gaccagcgag ttgatatcgg cgaagcccat ctcgtacgcg cccgaggcaa
      421 cccgattgat gccctcgacc gagcccgcgc caggatcgat cgagacattg agcccttcgg
      481 ccttgtagta gcccttgtcg atcgcgacga agtacggcgc cgccggccct tcgaagcgcc
      541 aatcgagcgc gaagcggatc gcggtttctt tctgtgccag cgcggcagtg gaaaaagcga
      601 gcgcggcgag agtcgctacg aagcgcatgg gagctcccct gaacgaagac ttttccggat
      661 aaagtctaca gcaccatgcg ctcgagcctg gagtttttcc tcaatgggga gcgagtgtcc
      721 ctccccgcga gccggggcac ccgaacggtg ctcgcctggc tgcgcgagga gcgccggctc
      781 accggcacca aggagggctg cgccgaaggc gattgcggcg cctgcaccgt ggtcgtctcc
      841 gagcccggct ccgatctgcc ggtctgcagc tgcatctacc tgctcggcgc gctcgacggc
      901 aaggcgctca ccacggtaga aggcctgaag ggccatccgg cgcagcgcgc gatggtggaa
      961 tgccacggct cgcaatgcgg cttctgcacg cccggcttcg tcatggcgct ctacgcgcac
     1021 taccgcaacc ccagcggcgc gctcgccgac gcgctcgccg gaaacctgtg ccggtgcacc
     1081 ggttacgcgc cgatcctggc ggcgggaaaa aggatgttcg agtacgcgca cgccacgagc
     1141 gaggaaagcc atgctcccca gcgagtaccc gcggagggcg tccaggaccg gtgcttcagc
     1201 gccccgcgca acctcgtcga gctcggtcgc gtgctgcaag gcggcgcgac gctgctcggg
     1261 ggcggtaccg atctggggct cgtcgtcacg aagcagttga aagattggga gcaggtggtc
     1321 tatctgggaa atgtggccga gctcaagagc atcgtagacc gtggttcgtt tctcgagatc
     1381 ggtggagcgg tcacgtattc gcaggcacat cccgcgctcg cggcgatcca tcccgacatc
     1441 ggcgagctcc tgcggcgcct cggcggcgcg caagtgcgcg cctgcgggac gattgccgga
     1501 aacgtcgcga acggctcgcc gatcggcgac tcgatgcctt ttctgctcgc gctgggcgca
     1561 accgtgctgc tgcaaaaggg cgagggccag cgcgaggtgc gacttgatac cttttatacc
     1621 gggtatcgca agagcgtcct ccagccgggc gaattcatac gcttgatccg cgtgcccagg
     1681 cttgctcccg gcgcgcgctt cgcggcgtac aaggtgtcca agcgctttga ccaggacatc
     1741 tcggcagtgt gcgcggcgtt ccatgtggaa ggagcccagg cgaggttcgg tttcggcggc
     1801 atggcggcga cgccctcgcg cgcgccgcgc gcagaagcgg ctttcgcgca aggagtcgaa
     1861 gccgcgtgcg ctgcgctcgc ccacgatttc aagccgctta gcgatcatcg cgcttcagcg
     1921 tggtaccggc tcaccctggc gcagaatctg ctgcgcaagt ttgcgcgcgg cgcttctcca
     1981 accgcgatct tcaagctgtg atccgcgagc cgttgccgca cgacagcgct tcgctgcacg
     2041 tctcgggcga ggcgcgctac gtcgacgatc tgcccgagcc gcccgggacg ctgcaggcgg
     2101 cgatcggggt gtcgaccgag acctttgcga agctcctctg cctcgacctc gaccccgtgc
     2161 ggcgcgcgcc cgaagttgtg gcggtaatca gcgcggccga tatcccgggg agcaacaaca
     2221 tcgggccggt gctgccggat gagccgattt tcgcgctgga caaggtcgag ttccgcggcc
     2281 agccgctgtt cgccgtggcg gcgaccagcg tcgaggctgc ccgtcgcgcg gcccggctcg
     2341 ctcgcgccga ataccagccg cacgagcctg tcgtcagcgt cgagcaggct ctcgaacgca
     2401 agcagttcgt cgtgccgtca atcgtcgtct cgcggggaac catccgccgc gattctccct
     2461 acagcgcaag aggcaagctc cgctgcgggg ggcaggagca cttttacctc gaaggccagg
     2521 tggcgctcgc gattcccggc gagcgcggcg caatgctcgt acattcgtcc actcagcatc
     2581 cgaccgaggt gcagcatctc gtggccgtgg cgctcggcgt gcccgacgcg agggtcaccg
     2641 tcgagctcag gcggctcggc ggcggcttcg gcggcaagga gacgcaggcc gcgctctatg
     2701 ccgtggtcgc ggcactcctc gcccgcgcca cccgccggcc ggtgaagctg cgcgccgacc
     2761 gcgacgacga catgatcggc accggcaagc ggcacgactt cctttacgaa tacgaagccg
     2821 ggttcgaccg cgagggacgc atcgaggcgc tcgacctgat gctcgcgtcg cgttgcggga
     2881 tctcggccga cctttccggc gcggtgaacg accgcgcggt gtttcactgc gacaacgcct
     2941 acttcctgcc caacgtgcgc attacctccc accgctgcaa gacgaatacc gtttctaaca
     3001 cagcgttccg cggcttcggc ggccctcagg gaatgtttgc agccgaaggc gtgatcgcag
     3061 ctattgcgcg ccgcctggga atcgacgcgc tcgacgtgcg caagcgcaat ctttacggcg
     3121 aagcgccgcg caacatcacc cattacggga tgacagtaaa ggacaacgtc cttccggaac
     3181 tcgtcgagga gctggagacc cgatccgaat atcggcgacg acgcgccgca atcgccgatt
     3241 tcaaccgcac gaacgaggcg tacaagaaag gcctggcgct cacgccggtc aagttcggta
     3301 tcgccttcac cacggtgtcg atgaaccagg caggggcgct ggtgcacatc tataccgacg
     3361 gcagcgtgca cctcaaccac ggcggcaccg agatgggcca ggggctgatg atcaaggtgg
     3421 cccagctcgt ggccgaggag ctcggcgttc cgctcgatcg cgtgcaaatc accccgacct
     3481 cgactgccca ggtgccgaac acctcgccga ccgcggcgtc gagcggcacc gacgtgaacg
     3541 gcgaggcggc gcgcgcggca tgccgggtgc tgaaggagcg tctcgcgcca ttccgggaaa
     3601 agtttcccga agcgagcttc gcggagctgg cgcgcgaggc gttcctcgcc cgcgtaccgc
     3661 tctcggcgac aggctattgg cgcacgccag acatccactt cgatcgcgag agcttcaccg
     3721 gcgagccgtt ctactacttc gcctatggcg ctgcggtttc cgaagtcgcg atcgatacgc
     3781 tcaccggcga gtggcgtttg ctgcgcgcgg acctgctcca tgacgtcggc cgctcgctca
     3841 acccggcgat cgaccggggg caggtcgaag gcggcttcat acagggcatg gggtggctca
     3901 cgatggaaga gctcgtgtgg gacgcgggcg gcgcgctcgc gacgcacgcc ccctccacct
     3961 acaagatccc cgtggcgggc gactgcccgc ggcagttcaa cgtctggctg tgggagcgcg
     4021 gcgagaatct gcgcgacgtc gcgtaccgtt ccaaggcggt cggcgagcca ccgctcatgc
     4081 tcgcgctgtc ggtgttccac gccatctacg acgcgatcgg cgccaccggc gattcgcgcg
     4141 acccagtgcc gctcgaagct cccgccacgc cggagaacat cctgagggcg atatgccaat
     4201 tgagatcctg atcgtcgaaa cccgcggctc cgcgccccgc gaggccggca cccggatgtg
     4261 ggtggacggc ggccagattc acggcagcat cggcggcggc aatctcgagt acaccgcgct
     4321 caagattgcg cgcgagatgc tgttgtccgg cgaagagttc aggcaacgga aattctctct
     4381 tggcgattcg ctcggccaat gctgcggcgg cagcgtgacg ctgcatttca ccaagaaagc
     4441 cctttttgtt ttttcagaga cgacctgctt tcacctcgca cttttcggcg ccgggcacgt
     4501 cggcaaggaa gtggcgcgca tcgtggagcg cctgccctgc aaggtgacgt ggatcgatcc
     4561 gcgtcccgat gtctttccgt caacagtagg cggcaacgta aaggttgtca tcgaagagga
     4621 gccggcgtgg atggtggatg aagcatccgc caacgcgtac tacctcgtca tgacgcacag
     4681 ccacgcgctc gacctcgaga tcgtagagcg ggtgctcgag cgaagagatg tggcgtttct
     4741 gggcctgatc ggctcgcaga ccaaggctgc gaagttccat ctcaggctga aagcaaaggg
     4801 catcgattcg tcgccgctgg tatgcccggt cgggctcttc aaggcgggca agcatcccgc
     4861 agaggtcgcc gtttccgccg tggcgcagct actcgcgcgg cgcgcgcagg tgccactcgg
     4921 tagcgcgctg gaacgcgtgg gcggcgcgta gcgcgcttgc atcgtcgtgc ggcggtgcga
     4981 tgagctgcat gccgatcggc atcccttctc tcgtaaagcc ggcgttggca acgacgaccg
     5041 gcagccccag gtaactgatc gggcgcgtga ggcgtacgaa gcttccgagc atctgcgccg
     5101 cggcttcgcc ggtcgagatg tccgtctgcg cgatggtcgg cgtctggaag gacaccaccg
     5161 gcccgtgcac cacgtcgacc tttgaaaaga ctgcctcgca gaacgccgcg atggccggag
     5221 cgcgcgcctg cagcgccttc acgtagtcga gcgcgagcgt gggccggccg cgctccagcc
     5281 gctcgcgcac gtgcgccgag tagtcctgcg gccgggtgcg gaaccattcg tcgtggaacg
     5341 gattcgcttc ggcggcgtgc agcacggccg ccagggcgcc cagcctggcg aaatccgcaa
     5401 gctcgatgtc cacgatcgtg gcgcccagcc ggcggtacac gtcgagcgat gcctccatcg
     5461 cggccgcaat ctcgggctcg cagccttcgt agaacacgtt gcgcggccgg ccgatgcgca
     5521 atccggagac aggcctttca agctctcgcg tcagatcggc ttgtgctatt acctccaaga
     5581 ccagagcagc gtcttccacc gtgcgggtga gcgggccaac cgtgtcgagc gatgggcaaa
     5641 gcggcaggat gttttcgacc gagaccaggc cgtgggtcgg gcgaatgccc gcaatgccgc
     5701 agaag
//
LOCUS       DASUNT010000546         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1124198, whole genome shotgun sequence.
ACCESSION   DASUNT010000546 DASUNT010000000
VERSION     DASUNT010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124198"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1991
                     /locus_tag="VFX94_11070"
     CDS             <1..1991
                     /locus_tag="VFX94_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5768766.1"
                     /translation="SVTGACSAEWLPIKPKTDAAFLFALIHVLLHEEVRSRLDVEFLY
                     RHTSSPYLVGTNGYYLRDPGYGQPIVAGSLEAKETHVALEIGLDGDVLAEGRLEGETA
                     FAKLLAHMKPYSPEWAQAICDVPAATIRRIAREYLDAARVGETIEIEGKRLPYRPVAV
                     SLGKTVTNGWGGYECCWARTLLTSLVGALEVPGGILGTTVRLNRPMSERHRSVKAGPD
                     GLMNYPFNPTDAAHWSSQPNIRNAYRSMVPLAADGPWSQALGPTHFSWMFLDETPPGL
                     PRVTLPELWFLYRTNPAISFWDNAALEKRMARFPFVVAFAFTRDETNHFADILLPDAG
                     DLESLQLIRIGGSKYIEQFWDHEGFALRQPAIAPRGQARDFTDIATDLAARCGLLESY
                     NAAINRGTLGVGLKDFSLEVARSHKSEEIWDAVCKAASSELTGGAEAHGLDWWKEHGL
                     ATRPFPRTAWYLYPTLAAKGLRFEMPYQERLARIGAELGRRLHEKGMRWWDKQLAEYQ
                     ALPAWKDFPGVWEASLAAMGGDGKAYPFWLLTARSMQYAWGGNVGMQLIKEVADNVAG
                     HNGVIINSAKAKELRIGDGDWIRIATPKAEVRARAVPRQGIRPDTLLLLGQFGHWATP
                     HARDFGMPSMNPLVPMSLELTDATGSGADLVRVSVNRI"
BASE COUNT          357 a          686 c          651 g          317 t
ORIGIN      
        1 tctcggtcac cggcgcctgc tccgccgagt ggctgccgat caagcccaag accgacgccg
       61 cgttcctgtt cgcgctcatc cacgtgctgc tgcacgagga ggtgcgcagc cgcctggacg
      121 tggaatttct ctatcgccac acctcgtcgc cgtatctcgt cgggacgaac ggctactacc
      181 tgcgcgatcc gggctacggc cagccgatcg tcgccggctc gctcgaggca aaggagacgc
      241 acgtcgcgct cgagatcggc ctggacggcg acgtgctcgc cgaaggccgg ctcgaaggcg
      301 agaccgcgtt tgccaagctc ctcgcgcaca tgaagcctta ctcgcccgaa tgggcgcagg
      361 cgatctgcga cgtgcccgcg gcgaccatcc ggcgcatcgc tcgtgagtac ctcgacgcag
      421 ctcgcgtcgg agaaacgatc gagattgaag gaaaacgtct accgtaccgg ccggtcgcgg
      481 tgagcctcgg caagacggtc actaacggct ggggcggcta cgagtgctgc tgggcgcgca
      541 cgctgctcac ctcgctcgta ggagcgctcg aagtcccggg cggcatcctc ggcactacgg
      601 tgcggctgaa ccgcccgatg tcggagcgcc accgcagcgt gaaggcgggc cctgacgggc
      661 tgatgaacta ccccttcaac ccgactgacg ccgcgcactg gagctcgcag ccgaacatcc
      721 gcaacgcgta tcgctcgatg gttccgctcg ccgccgacgg cccctggagc caggcactcg
      781 gcccgacgca tttctcgtgg atgttcctcg atgagacgcc gccgggactg ccgcgcgtga
      841 cccttcccga gctctggttt ctctaccgta ccaacccggc gatctcgttc tgggacaacg
      901 ccgcgctcga gaagcgcatg gcgcgtttcc ccttcgtcgt cgcgttcgcc tttacccgcg
      961 acgagaccaa ccatttcgcc gacatcctgc ttcccgatgc gggcgacctg gagagcctgc
     1021 agctcatccg catcggtggc tcgaaataca tcgagcagtt ctgggaccac gaagggttcg
     1081 cgctcaggca gccggccatc gcgccgcgcg ggcaggcccg cgacttcacc gacatcgcga
     1141 ccgacctcgc cgcgcgctgc gggcttctcg agagctacaa cgccgcgatc aatcggggaa
     1201 cgctgggggt tgggctcaaa gacttcagcc ttgaggttgc ccgaagtcac aaaagcgaag
     1261 aaatctggga cgcggtgtgc aaggcggcaa gcagcgagct caccgggggc gccgaggccc
     1321 acgggctcga ctggtggaaa gagcacggcc tcgccacccg gccgttcccg cgcaccgcct
     1381 ggtacctcta cccgacgctt gcagccaagg gcctgcgctt cgagatgccc taccaggagc
     1441 ggttggcgcg catcggagcc gagctcggac gccgcctgca cgagaagggc atgaggtggt
     1501 gggacaagca gctcgccgag taccaggcgc tgcccgcgtg gaaggacttc cccggcgtat
     1561 gggaagcctc gctcgccgcg atgggcggcg acggcaaggc atacccgttc tggctcctca
     1621 ccgcgcggtc aatgcaatac gcgtggggtg gaaacgtcgg aatgcagctc atcaaggaag
     1681 tcgccgacaa cgtggccggc cataacggcg tgattatcaa ttctgcaaaa gccaaggagc
     1741 tgcgaatcgg cgacggcgat tggatccgca tcgcaacacc caaagccgag gtccgcgcgc
     1801 gcgcggtccc gcggcaggga atccgccccg acacgctgct gctgctgggg cagttcggcc
     1861 actgggcgac gccgcacgcc agagacttcg gcatgccgtc catgaacccg ctggtgccga
     1921 tgtcgctgga gctcaccgat gcgaccggtt caggcgccga tctggtgaga gtttcagtaa
     1981 acagaatatg actcgttggg ccatggtcgc c
//
LOCUS       DASUNT010000547         5419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1124467, whole genome shotgun sequence.
ACCESSION   DASUNT010000547 DASUNT010000000
VERSION     DASUNT010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124467"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1893)
                     /gene="ftsH"
                     /locus_tag="VFX94_11075"
     CDS             complement(13..1893)
                     /gene="ftsH"
                     /locus_tag="VFX94_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX5768767.1"
                     /translation="MLKNLVIWLVIGLVLMTVFNQFNTRQSAQAPMEYSQFLEEVKAG
                     RIAKVTIEGRQLKATTSEGKRVTSYSPGDIWLVSDLLKYGVKIEAKPEEEPSLLMNIF
                     VSWFPMLLLIGVWIFFMRQMQGGGRGGAFSFGKSRARMLDESTNTVTFADVAGCEEAK
                     EEVAELVDFLRDPSKFQKLGGRIPRGVLMVGNPGTGKTLLARAIAGEAKVPFFSISGS
                     DFVEMFVGVGAARVRDMFEQAKKHAPCIVFIDEIDAVGRQRGAGLGGGNDEREQTLNQ
                     LLVEMDGFESTMGVIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDIRGREEILKVHM
                     RKVPLAPDVKPDILARGTPGFSGADLANLVNEAALFAARGNKRLVDMDDFERAKDKII
                     MGAERRSMVMPEEERRNTAFHESGHAIVARLLPKTDPVHKVTIIPRGRALGVTMQLPE
                     SDRYSQDRDRLLNMIAVLFGGRIAEELFMNQMTTGASNDFERATEIARRMVTQWGMSD
                     ELGPMVYGENEGEIFLGRSITTHKNVSEATMQKVDAEIRRIIDQQYRLARRLLEDNRD
                     KVEAMAKALMEWETLDSDQINDIMAGKPPRPPKPTSAPSSGPSSGGTPAQPEPAPTTT
                     PA"
     gene            complement(1931..2521)
                     /locus_tag="VFX94_11080"
     CDS             complement(1931..2521)
                     /locus_tag="VFX94_11080"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEX5768768.1"
                     /translation="MAKSSKAWLRRHVSDPYVKQARKQGYRSRAAFKLIEIDSRERLF
                     RPGQTVIDLGAAPGGWSQVAAEKVTPQGRVIAIDVLEIAPIFGVTVHKGDFRRISLES
                     RADVVLSDLSPNMSGTPNVDQARWLELAEGAVELCRKILKPDGVFLVKAFHGEAFDEL
                     MEHLKTAFVKVKVVKPSASRGESAETYVVARGLRAL"
     gene            complement(2811..3833)
                     /locus_tag="VFX94_11085"
     CDS             complement(2811..3833)
                     /locus_tag="VFX94_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5768769.1"
                     /translation="MKLLLLALAAMLAACAAVSFPYFDPAKPHRAADGFRNNYPHPEK
                     GGFWRWKWDQLRGGLPRNPEEGYRFAAATPNLVANPSVTWVGHATVLLRVGGLSVLTD
                     PHFSERASPVTFAGPRRVVSPVPALNELPHIDAVVVSHNHYDHLDHDSVARLAEQAGG
                     SPRFFVPLGLKDWFARRGIQDVVELDWWESLDFRGLKVDFVPVQHWSKRTLTDENQTL
                     WGGWVIRHPELSFFFAGDTGYSKDFADIRARFGGFDLAAIPIGAYAPRWFMQIMHLDP
                     AEAVRVHQDVNARRSLAIHWGTFANLTDESLYEPPLRLVEERKKAGLADDAFLVLRHG
                     ETHVLR"
     gene            complement(4001..4183)
                     /locus_tag="VFX94_11090"
     CDS             complement(4001..4183)
                     /locus_tag="VFX94_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768770.1"
                     /translation="MPLRTAFWQSAAQSLAPSVRNRYAADLELAESVDLFIAGLIDAW
                     RRARVALMRGRRKAYS"
     gene            complement(4277..4435)
                     /locus_tag="VFX94_11095"
     CDS             complement(4277..4435)
                     /locus_tag="VFX94_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768771.1"
                     /translation="MKKILPYVLVETFLPGGSLIALGMWLYQNRDRLPKWRGRAHGAS
                     DAPAPSAT"
     gene            complement(4507..>5419)
                     /locus_tag="VFX94_11100"
     CDS             complement(4507..>5419)
                     /locus_tag="VFX94_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768772.1"
                     /translation="GRLTPGTKAPLRTSPIALLRRENVSAWGMEKGAVDLSSEAAAVR
                     KALEARGASFFHELVANTRLLPSLVERGLAELAGAGIATADSFAGLRALLAAPEKRRR
                     LVETAGRWSLLRNEKSDEVEAVARTLLKRYGIVFRTLLQRESRLPPWRDLVRVYRRLE
                     ARGEIRGGRFVGGFGGEQFAAPDAVGRLRAVRKRERIEELVVLSAADPLNLVGILTPE
                     ARVAAVHRNRVLLRDGLPIAAVEGGQLRRLADSELDDSRLRTLLARRSLRHPPALHVR
                     TPTPGETAWLERARDRMRNYPWSQVRR"
BASE COUNT          904 a         1781 c         1803 g          931 t
ORIGIN      
        1 ctgcgtgacg gattacgctg gcgtggtcgt cggggcaggc tccggctgcg ccggggtccc
       61 gccggacgaa ggaccggacg aaggcgccga cgtgggcttg ggcggacgcg gcggcttgcc
      121 cgccatgatg tcgttgatct ggtcggagtc cagcgtctcc cactccatga gcgccttcgc
      181 catcgcctcg accttgtcgc ggttgtcctc gaggagccgg cgcgcgaggc ggtactgctg
      241 gtcgatgatg cggcggatct cggcgtcgac cttctgcatc gtcgcttccg agacgttctt
      301 gtgcgtggtg atcgagcggc cgaggaagat ctcgccctcg ttctcgccgt agaccatcgg
      361 gccgagctcg tcggacatgc cccactgcgt gaccatgcgg cgcgcaatct cggtcgcgcg
      421 ctcgaagtcg ttcgaggccc cggtggtcat ctggttcatg aagagctctt cggcgatgcg
      481 gccgccgaag agaaccgcga tcatgttgag caggcggtcg cggtcctggc tgtagcggtc
      541 gctctcggga agctgcatcg tcacgccgag cgcgcggcca cgcgggatga tcgtcacctt
      601 gtgcaccggg tcggtcttgg gcagcaggcg cgcgacgatc gcgtggccgg actcgtggaa
      661 agcggtgttc ctgcgctctt cctcgggcat gaccatggag cggcgctcgg cgcccatgat
      721 gatcttgtcc ttggcgcgct cgaagtcatc catgtcgacg aggcgcttgt tgccgcgcgc
      781 ggcgaagagc gccgcctcgt tcaccaggtt agcgaggtcg gcgcccgaga acccgggcgt
      841 gccgcgcgcg aggatgtcgg gcttgacgtc cggcgcaagc ggcaccttgc gcatatggac
      901 tttgagtatt tcctctctgc ctcttatgtc ggggagggga acgacaactt gccggtcgaa
      961 gcggccggga cggaggagcg ccgggtcgag cacgtcgggg cggttggtcg ccgcgatgac
     1021 gatcacgccc atggtggact cgaagccgtc catctcgacg agcagctggt tcagcgtctg
     1081 ctcgcgctcg tcgttgccgc cgcccaggcc ggcgccgcgc tggcggccga ccgcgtcgat
     1141 ttcatcgatg aacacgatgc acggcgcgtg cttcttcgcc tgctcgaaca tgtcgcgcac
     1201 gcgcgccgcg ccgacgccga cgaacatctc gacgaaatcc gagcccgaga tggaaaagaa
     1261 cgggaccttg gcctcgccgg cgatggcgcg cgcgaggagc gtcttgcccg tgccggggtt
     1321 gccgaccatg agcacgccgc gcggaatgcg gccgccgagc ttctggaact tggacggatc
     1381 gcgcaggaag tcgacgagct cggcgacctc ctccttcgcc tcctcgcagc ccgcgacgtc
     1441 ggcgaaggtg acggtgttgg tcgattcatc gagcatgcgc gcgcgcgact tgccgaacga
     1501 gaaggcgccg ccgcgcccgc cgccctgcat ctggcgcatg aagaagatcc acacgccgat
     1561 gagcagcagc atcgggaacc aggagacgaa gatgttcatg aggagcgagg gctcctcctc
     1621 gggcttcgct tcgatcttga cgccgtactt gaggagatcg ctcaccagcc agatgtcgcc
     1681 cggcgaatag ctggtgacgc gcttgccttc gctggtcgtg gccttgagct ggcggccttc
     1741 gatcgtgacc ttggcgatgc ggccggcctt cacttcttcg aggaactgcg agtactccat
     1801 cggcgcctgg gccgactggc gggtgttgaa ctggttgaac acggtcatca gcaccagccc
     1861 gatgacgagc cagatgacca ggttcttcag catgttattc aagtgcttga ctcctgccta
     1921 aaaaatcatc ttacagggcg cgcaagccgc gtgccaccac gtaagtctcc gccgattcac
     1981 cccgtgaagc cgagggtttt acaactttca ccttcacgaa cgctgtcttc aggtgctcca
     2041 tcagctcgtc gaacgcctcg ccatgaaagg ctttgaccag gaaaacaccg tcgggcttca
     2101 ggatcttgcg gcaaagctcg acggcgcctt cggcgagctc aagccagcgc gcctggtcga
     2161 cgttgggagt gcccgacatg ttcggcgaca ggtcggacag caccacgtca gcgcgcgatt
     2221 cgagcgaaat gcggcggaaa tcccccttgt ggacggttac cccgaaaatg ggggcgatct
     2281 ccagcacatc aatggcgatc acccgcccct gcggcgtcac cttctccgcc gctacctgcg
     2341 accagccgcc cggcgcggcg ccgaggtcga tcaccgtctg gccgggccgg aaaagcctct
     2401 ccctggaatc tatttcaatc agtttgaaag cggcgcggga ccggtagccc tgcttgcgtg
     2461 cctgcttgac gtaagggtcg ctgacgtgcc ggcgaagcca cgccttgctc gatttcgcca
     2521 cgtgcaaagc ttaccgtgct cgcttctgca cggtccgcgg gatggtgtat tcacgtgcct
     2581 gcgcgaagcg ctgaacgccg agctttccgc gacgccgtct cgcgtcggtc cttgccgcta
     2641 cctcgagctc gccgtcgcag ctcaccgcgg ccggggacgc tcgggtgcgc cttaccggcg
     2701 aagcgcgcgt cgacgaaaag ctctggacgc tataccaagg cagccggcgg gcaggtcgat
     2761 ctggcaagct gcggcgtcaa gtagctcgac gccgtctcgc gctgacctcg tcagcgcaat
     2821 acgtgtgtct cgccgtgccg caggacgagg aacgcgtcgt cggcgaggcc ggcctttttc
     2881 cgctcctcga ccagccgcag cggcggctcg tacaggctct cgtcggtgag gttcgcgaac
     2941 gttccccagt ggatcgcgag cgaccggcgc gcgttcacgt cctggtgcac acgcaccgct
     3001 tcggctgggt cgaggtgcat gatctgcatg aaccagcgcg gcgcgtaggc gccgatcggg
     3061 atcgccgcca ggtcgaaccc gccgaagcgg gcccggatgt cggcgaaatc cttcgaatag
     3121 ccggtgtccc ccgcgaagaa gaacgagagc tcggggtgcc ggatcaccca cccgccccaa
     3181 agcgtctggt tttcgtcggt cagcgtgcgc ttcgaccagt gctgaacggg cacgaagtcg
     3241 acctttaggc cgcgaaaatc gagcgattcc caccaatcga gctccaccac gtcctggatg
     3301 ccgcgccgtg cgaaccagtc cttcagaccg agagggacga agaagcgcgg cgagccgccg
     3361 gcctgctccg cgagccgtgc cacgctgtcg tggtcgaggt gatcgtagtg attgtgcgaa
     3421 acgacgacgg catcgatgtg cggtagctcg ttcagcgccg gcaccggcga caccacgcgc
     3481 cggggcccgg caaaggtgac cggcgaagcg cgctcggaaa aatgcgggtc ggtgagcacg
     3541 ctgagcccgc ctacgcgaag cagcacggtg gcgtggccaa cccaggtcac cgagggattg
     3601 gccaccaggt tgggagttgc ggctgcgaac cgatagccct cctcgggatt gcgggggagg
     3661 cctccgcgca gctggtccca cttccagcgc cagaacccgc ccttctccgg atgtgggtag
     3721 ttgttgcgaa agccatcggc cgcgcgatgc ggcttggccg gatcgaagta aggaaagctc
     3781 acggcggcgc aggcggcgag catcgccgcg agcgccaaga gtagcagctt catgccaggg
     3841 cggacctggc ctccacgtta catccgaaat gcatctcgag ccagccttcg gcttcgcggc
     3901 gctcagcgtc gtacaacctc ttcatgaatc tcgagtgggt attgagccga ctcagcatgc
     3961 tcagctgatt caagttctcc ggcgtgcagg agatgcacgt tcagctgtag gccttgcgcc
     4021 gcccgcgcat gagcgccacg cgcgcccgcc gccaggcgtc gatcagcccg gcgatgaaga
     4081 gatctacgct ctcggcgagc tcgaggtcgg ctgcataccg attgcgcacc gaaggggcga
     4141 gtgattgagc ggcgctctgc cagaatgcgg ttcgaagggg cattgacgga ctccgtgtga
     4201 cccgtatgca agccacggta ggcgcaagtg ctcggcctaa caaacgagga ttagtcaacc
     4261 accgctttag aaacgctcaa gtcgcgctcg gtgctggtgc gtcggatgca ccgtgcgcgc
     4321 gaccgcgcca cttcggcagg cggtccctgt tctggtagag ccacatgccg agcgcgatca
     4381 gcgagccgcc gggcaggaag gtttctacga gaacataggg aagaatcttt ttcacggcgc
     4441 cagtctggcg cgggcgcgtt gcgcgtccat gtccggcggc cgtgccttcg ttacatccga
     4501 aacgtcctag cggcggacct ggctccaggg atagttgcgc atgcgatcgc gcgcgcgctc
     4561 gagccacgcc gtttctcccg gcgttggcgt gcgcacatgg agcgccggcg ggtggcgcag
     4621 cgaacggcgc gcgagcaagg tcctcagcct cgaatcatcg agctcggaat cagcgagccg
     4681 gcgcagctgc ccgccctcca ccgcggcgat cggcaggcca tcgcgcaaca gcacgcgatt
     4741 gcggtggacc gccgccactc gcgcctcggg cgtgaggatg ccgacgaggt ttagcggatc
     4801 ggcggcgctc aggacgacaa gttcttcaat cctttcgcgc ttgcgcaccg cgcgcagtcg
     4861 tccgacggca tcgggcgcgg cgaactgctc gccgccaaag ccgccgacga agcggccgcc
     4921 gcggatctcg ccgcgcgctt ctagcctgcg gtagacgcgc accagatcgc gccacggcgg
     4981 caggcgcgat tcgcgctgga gcaacgtgcg aaaaacaata ccgtaacgct tgagcaacgt
     5041 tctcgccacg gcttcgacct cgtcgctctt ctcgttcctg agaagcgacc agcgcccggc
     5101 agtttccacc agtcgccggc gcttttccgg cgcggcgagg agcgctctca agccggcgaa
     5161 gctgtctgcg gtcgcgatgc cggcaccggc gagctccgcg aggccgcgct ccacgaggct
     5221 cggcaagagc cgagtgtttg caaccaattc atggaagaac gaagcgccgc gcgcctcgag
     5281 cgctttgcgc acggcggccg cctcggacga gaggtctacc gcgcccttct ccatgcccca
     5341 ggcggaaacg ttctcccggc gaagcaacgc gatcggcgac gtgcgtagcg gtgccttggt
     5401 tcccggcgtg agacgtccc
//
LOCUS       DASUNT010000548         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_511992, whole genome shotgun sequence.
ACCESSION   DASUNT010000548 DASUNT010000000
VERSION     DASUNT010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511992"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VFX94_11105"
     CDS             complement(<1..670)
                     /locus_tag="VFX94_11105"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX5768773.1"
                     /translation="MTNHPPLERLMLFTGNANPRFAQDVAKGLNLSLGRAIVGKFSDG
                     EVMVELLENVRGKDVFVLQSTCAPTNDNLMELLVMIDALKRSSAARVTAAIPYFGYAR
                     QDRRPRSARVAISAKVVANMLASVGVARVLTMDLHADQIQGFFDIPVDNIYAAPVLLG
                     DVWKQRYENLIVVSPDVGGVVRARALAKRLESDLAIIDKRRPRPNVATVMNIIGDVAG
                     RTCVI"
     gene            complement(699..775)
                     /locus_tag="VFX94_11110"
     tRNA            complement(699..775)
                     /locus_tag="VFX94_11110"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(739..741),aa:Gln,seq:ttg)
     gene            complement(790..1614)
                     /gene="ispE"
                     /locus_tag="VFX94_11115"
     CDS             complement(790..1614)
                     /gene="ispE"
                     /locus_tag="VFX94_11115"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008110855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX5768774.1"
                     /translation="MELIWYPAPGKLNLFLHVLERRADGYHDLQTAFRLIDRADRVGV
                     AARDDGKITFEGPFGEDNLCVRAARLLKAEARSTVGCHISLEKNLPVGGGLGGGSSDA
                     ATVLLVLNKLWGFGLSRERLLQLALALGADVPFFIYGRNALGEGIGERLRPLDVPPAW
                     YLVVTPQVSVSTRETFESALTHRSKRLKIPPFFSGQGANDLESAVVARYPEVAASLAW
                     LRQQRPQARMTGSGACVFAEFETEAEARALHSALPGGMRGFVARGLEQHPLYDIVN"
     gene            complement(1605..>2121)
                     /locus_tag="VFX94_11120"
     CDS             complement(1605..>2121)
                     /locus_tag="VFX94_11120"
                     /inference="COORDINATES: protein motif:HMM:NF015512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEX5768775.1"
                     /translation="DPAEDHQAAAAVRIVVLAVVFVAACAQVEIKPPEGPLEFSLAGR
                     IAARYGGESFTGNIAWRHARGGDELLISTPTGQGVAQIVRQGEAVLLKTAEPREYRDN
                     DAEALTERVLGFRIPIDGLADWVQGKPSPQFEGRGWKVEYQDYDAERRPTRMRLTYQG
                     IELRLAISQWN"
BASE COUNT          361 a          709 c          679 g          372 t
ORIGIN      
        1 tgatcacgca ggtgcggccg gccacatcgc cgatgatgtt catcaccgtg gccacgttgg
       61 gccgcgggcg ccgcttgtcg atgatcgcga ggtccgactc caggcgcttc gcgagcgccc
      121 tcgcccgcac cacgccgccc acgtccggcg ataccacgat cagattctcg tagcgctgct
      181 tccacacgtc gccgagcagc acgggcgcgg cgtagatatt gtcgaccggg atgtcgaaga
      241 agccctggat ctggtcggcg tgcaggtcca tcgtgagtac ccgcgccacg cccaccgagg
      301 cgagcatgtt ggcgacgacc ttggcgctga tcgccacccg ggccgagcgc ggccggcggt
      361 cctggcgtgc gtagccgaag tacgggatcg ccgcggtgac gcgcgccgcc gaggagcgct
      421 tcagcgcgtc gatcatcacc aggagctcca tcaggttgtc gttggtcggc gcgcacgtgg
      481 actgcagcac gaagacatcc ttgccgcgca cgttctcgag cagctcgacc atcacctcgc
      541 cgtcggagaa cttgcccaca atcgcgcgcc cgagcgacag gttcaggcct ttcgccacgt
      601 cctgagcgaa gcgcgggttg gcgttgccgg tgaacagcat taaccgctca aggggcgggt
      661 ggttcgtcat cgcgctgttc atgaccgtca tgggttaatg gctggggagg aaggattcga
      721 accctcgtat ggcggaatca aaatccgctg ccttaaccag cttggcgact ccccagtgtg
      781 ctaccagagt caattcacaa tgtcgtagag cggatgctgc tccagtcccc gcgctacgaa
      841 acccctcatt cccccgggta acgccgaatg cagcgcgcgc gcttccgctt cggtttcgaa
      901 ttcggcgaag acacacgctc cggatcccgt catgcgcgcc tgcgggcgct gctgcctgag
      961 ccacgcgagg ctcgctgcca cctcgggata ccgggcaacg accgcagact cgagatcgtt
     1021 cgctccttgc cccgagaaaa agggcggtat tttgagtcgt ttcgaccgat gtgtcaacgc
     1081 ggattcgaac gtttctcttg tggaaaccga gacttgcggc gtcacgacca gataccaggc
     1141 agggggcaca tcgaggggcc gcaggcgctc gccgatgcct tcgccgagcg cgttgcgccc
     1201 gtagatgaag aacggcacgt cggcgccgag cgctagcgca agctgcagca gcctctcccg
     1261 cgacaggccg aatccccaaa gcttgttgag cacgagaagt acagtggccg catcggagga
     1321 gccgccgccg aggcccccgc cgacaggcag gttcttttcc agactgatat ggcacccgac
     1381 ggtgcttcga gcctctgctt tgagcagccg ggcggctcgc acgcagaggt tatcttcgcc
     1441 gaaaggcccc tcgaacgtga tcttcccgtc gtcccttgct gcgacaccca cgcgatcggc
     1501 gcggtcgatc agccgaaaag ccgtctggag gtcgtgatag ccgtctgcgc ggcgctcgag
     1561 cacgtgcaga aagagattca gctttcccgg cgcgggatac caaatcaatt ccactgcgat
     1621 atggccagcc gcagttcgat accctggtac gtcaggcgca tccgcgtggg gcggcgttcg
     1681 gcgtcgtagt cctggtattc gaccttccag ccgcggcctt cgaactgagg cgaaggcttg
     1741 ccctgcaccc agtcggcgag gccgtcgatc gggatccgga agccgagtac ccgctcggtc
     1801 agcgcctcgg cgtcgttgtc gcggtattcg cgcggctcgg cggtcttcag caggacggcc
     1861 tcgccctggc gcacgatctg cgccacgccc tgcccggtcg gcgtgctgat gagcagctcg
     1921 tcgccgccgc gcgcatgccg ccacgcgatg ttgccggtga acgattcgcc tccgtagcgc
     1981 gcggcgatgc ggccggcgag cgagaactcg agcggacctt cggggggctt gatttcgact
     2041 tgcgcgcaag cggcgacgaa gaccaccgcc agcacaacga ttctcaccgc cgcagccgct
     2101 tgatggtctt ctgcagggtc t
//
LOCUS       DASUNT010000549         5165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_512069, whole genome shotgun sequence.
ACCESSION   DASUNT010000549 DASUNT010000000
VERSION     DASUNT010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512069"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VFX94_11125"
     CDS             <1..849
                     /locus_tag="VFX94_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX5768776.1"
                     /translation="EANDHFRVTDDRAQLHTRVAMNSGVIASSLFAAADEAGIPDSIA
                     MQLGDVFGGDVDFHRDLRKGDRFAVVYELHYLGGRPVRAGRLLAAEFTNNGRTFRAVH
                     FNNGYYTPEGNNLRKAFLRSPLEFSRVSSGFGLRRHPIAQAWVKHQGIDYAAPTGTRV
                     RAVGDGIVEHAGLKGGYGKVVIIRHHGQYSTLYAHLSRLNVHRGQRVAQNDTIGAVGQ
                     TGWATGPHLHYEFHIAGQSRNPLSLTLPAALPVAAQDLAAFRNHAEPLIARLDLVANS
                     KLAQLE"
     gene            complement(908..1258)
                     /gene="erpA"
                     /locus_tag="VFX94_11130"
     CDS             complement(908..1258)
                     /gene="erpA"
                     /locus_tag="VFX94_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX5768777.1"
                     /translation="MNAVTEMPAPLVFSDSAANKVKQLIEEEGNPGLKLRVFVTGGGC
                     SGFQYGFTFDEVQNEDDAAMEKNGVTLLIDPMSYQYLAGAEIDYQEGLEGAQFVIKNP
                     NAQSTCGCGSSFSA"
     gene            complement(1277..1675)
                     /locus_tag="VFX94_11135"
     CDS             complement(1277..1675)
                     /locus_tag="VFX94_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX5768778.1"
                     /translation="MFNKTSKPQNRIDSLIGATTRIEGNVIFSGGLRVDGLVRGNVAA
                     LPDQPGTLVVSSDARIDGEVQAAHIVVSGTIHGPVHATETLELQAGSRVKGDVYYKSI
                     EVQQGAVVEGRLVHHPAEMKAVELKLASGG"
     gene            complement(1706..2461)
                     /locus_tag="VFX94_11140"
     CDS             complement(1706..2461)
                     /locus_tag="VFX94_11140"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HEX5768779.1"
                     /translation="MPSGRWKLIRQRFGIAAPRVEVQTQIPWYWRWAGIAMLLGVAAA
                     AASWIYDAGRRFAGFDRSEVLQELKRVQSELDAARGELEQLRAVANAADSRVSIERTA
                     QQKLAQQVRALEQENVRVRQELAMVEQMLTSEERHTQSLAIYQFKVEPAVLPGEYRYR
                     LLLLTPTDRRGRDFNGRLELVVNLQEGGRNAMMSFPEPGDGAAAAFRLAFKYFRRVEG
                     TFRVNPKAKVESVQVRVYEAGSSQPRATHSVTL"
     gene            complement(2478..3518)
                     /gene="argC"
                     /locus_tag="VFX94_11145"
     CDS             complement(2478..3518)
                     /gene="argC"
                     /locus_tag="VFX94_11145"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX5768780.1"
                     /translation="MIKAGIVGGTGYTGVELLRLLAQHPGVELKAITSRKEAGTPVAR
                     MFASLRGRVELAFSEPSARALAGCDVVFFATPNGVAMGEARAVLEGGARVVDLSADFR
                     IKDVAEWERWYKTKHAAPELVREAVYGLPELNREEIRRARLVANPGCYPTAVQLGFLP
                     LVEAGVVDLEHLIADAKSGVSGAGRKTELNLMFSEASDNFAAYHVAGHRHWPEIRQGL
                     AQAAKREVGLVFTPHLTPLIRGIHATLYARIVREMDFQQLFEKRYAGEPFVDVMPAGS
                     HPDTRSVRAANTCRLAVHRPPESARRSDTLVVLSVIDNLVKGASGQAVQNMNLMFGLA
                     ESTGLDQVAVVP"
     gene            complement(3571..3963)
                     /gene="rpsI"
                     /locus_tag="VFX94_11150"
     CDS             complement(3571..3963)
                     /gene="rpsI"
                     /locus_tag="VFX94_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX5768781.1"
                     /translation="MVGDYYYGTGRRKSAVARVFLKPGKGEFKVNGKPVDQFFGRETG
                     RMVVRQPLDLTKNLASFDIMVNVYGGGESGQAGAVRHGIARALIEYDAGLKSALSAAG
                     LVTRDAREVERKKVGLHKARRRKQYSKR"
     gene            complement(3953..4393)
                     /gene="rplM"
                     /locus_tag="VFX94_11155"
     CDS             complement(3953..4393)
                     /gene="rplM"
                     /locus_tag="VFX94_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX5768782.1"
                     /translation="MTTYTAKTGEVPQGWYVVDAQNKVLGRLAAQIAVRLRGKHKPQY
                     TPHIDTGDYIIVVNAAKLRVTGRKTERKIYYRHSGQPGQLRQTSFAKLHAKRPERVLH
                     IAVKGMLPKGPLGYAMLRKLKIYGGATHPHSAQQPKALEICDGR"
     gene            4446..4865
                     /locus_tag="VFX94_11160"
     CDS             4446..4865
                     /locus_tag="VFX94_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX5768783.1"
                     /translation="MNCTVRWMGEGMSFIAETGSKHLVAMDGAPDGGGRNLAPRPMEM
                     VLAGSGGCAAYDVVLILRKSGQDVTGCEVKLAAERASSDPKVFTRVHMHFVVRGRALK
                     RNLVEHAIRLSHEKYCSATAMLVKTAEVTRDFEIMEA"
     gene            4865..>5165
                     /locus_tag="VFX94_11165"
     CDS             4865..>5165
                     /locus_tag="VFX94_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768784.1"
                     /translation="MRPILALVLACSAALAAAQLRTIPKEAIPGKLRHLQAMVVEIDG
                     KPRQLAPGAQIRDTDNRLVLPASLVQKTVVGYTLDGAGSVHRVWILSPAERARLPK"
BASE COUNT          826 a         1776 c         1628 g          935 t
ORIGIN      
        1 gaggcgaatg accacttccg cgtcaccgac gatcgcgccc agctacacac ccgtgttgcc
       61 atgaattccg gcgtcatcgc gagctcgctt ttcgccgcag ccgacgaggc cggcatcccg
      121 gacagcatcg caatgcagct cggcgacgtg ttcggcggcg acgtcgactt tcaccgcgac
      181 ttgcgcaagg gcgatcggtt cgccgtggtc tacgagctcc attacctcgg cggccgcccg
      241 gtgcgcgccg gccggctgct tgccgccgag ttcaccaaca acggcaggac attccgcgcg
      301 gtgcacttca acaacggcta ttacaccccc gagggcaata atctccgcaa ggcgttcctt
      361 cgctcgccgc tcgaattctc gcgcgtgagc tcggggttcg gactgcgccg ccatccgatc
      421 gcgcaggcgt gggtcaagca ccagggaatc gactacgccg cgccgacggg cacccgcgtg
      481 cgtgcagtgg gcgacggcat cgtcgagcac gccggcctca aaggcggtta cggcaaggtg
      541 gtcatcatcc gccaccacgg ccagtactcg accctgtatg cgcacctcag tcgcctcaac
      601 gtgcaccgtg gccagcgggt cgcgcagaac gacaccatcg gggccgtcgg ccagaccggc
      661 tgggcgacgg gtccccatct gcactacgag ttccatatcg ccggccagtc gcgcaatccg
      721 ttatcgctca ccctgcccgc cgcgttgccg gtggcggcgc aggacctcgc ggcgttccgc
      781 aaccacgcgg agcccctcat cgcgcggcta gatctcgtgg cgaacagcaa gctcgcccag
      841 ctggagtaga aggagtagaa aagcaaaggg cccctttcag gggcccttgc gtgttgaatg
      901 ttgactgtta ggcggagaag gacgacccgc agccgcaggt gctctgggcg ttcgggttct
      961 tgatcacgaa ctgcgcgccc tccaggcctt cctggtagtc gatctcggcg ccggcgaggt
     1021 actgatagct catcgggtcg atgagcagcg tgacgccgtt cttttccatc gccgcgtcgt
     1081 cctcgttctg tacctcgtcg aaggtgaagc cgtactggaa gcccgagcat ccgccgccgg
     1141 tgacgaacac gcggagcttg aggccggggt tgccttcctc ttcgatcagc tgcttgacct
     1201 tgttcgcggc actgtcgctg aagaccagcg gcgccggcat ttcggtgact gcgttcattc
     1261 aacgttcctt tgcaaattac ccgcccgagg cgagcttgag ctccactgcc ttcatctcgg
     1321 cgggatgatg caccaggcgc ccttccacca ccgcgccctg ctggacttcg atgctcttgt
     1381 agtacacgtc ccctttgacc cgcgagcctg cctgtagttc gagcgtctcg gtggcatgca
     1441 ccggtccgtg gatcgtgccg cttacgacga tgtgcgccgc ctgcacttcg ccgtcgatgc
     1501 gcgcatcgga ggaaaccacc agcgtgcccg gctggtcggg gagcgcagcg acgttgccgc
     1561 gcaccagccc atccacacgc agcccgccgc tgaagatcac attgccctcg atgcgcgtag
     1621 tggcgccgat caggctgtcg atccggtttt gcggcttgct cgtcttgttg aacactccga
     1681 tctccctctc cctcctcgct tgcgcttaca acgttaccga gtgtgtcgcg cgcggctggc
     1741 tcgatcccgc ctcgtagacg cgcacctgca cactctccac tttcgccttc gggttgaccc
     1801 ggaaagtccc ttccacccgt cggaaatact tgaaagccag ccggaacgcc gcagcggcgc
     1861 cgtctcccgg ctccgggaag gacatcatag cattccgccc cccctcctgc aggttgacga
     1921 ccagctcgag ccgaccgttg aagtcgcgcc cgcgccggtc ggtcggcgtg agaagcagca
     1981 ggcgatagcg gtattccccg ggaagtacgg ctggctccac cttgaattgg taaatcgcga
     2041 ggctttgagt gtgacgttct tccgatgtga gcatctgctc caccatcgcg agctcctgcc
     2101 gcacgcggac attttcctgc tcgagcgcgc gtacctgctg ggcgagcttc tgctgcgcgg
     2161 tgcgctcgat tgacacgcgg ctgtcggcgg cgttggcgac cgcgcgcagc tgctccagtt
     2221 ccccgcgtgc cgcatcgagc tcgctctgca cgcgtttgag ctcctgcaac acttcgctgc
     2281 gatcaaagcc tgcgaagcgc cggcccgcgt catagatcca ggacgcggcg gccgccgcca
     2341 caccgagcag catggcgata cccgcccagc gccagtacca cgggatctgg gtctggacct
     2401 ccacgcgcgg ggctgcgatg ccgaagcgct ggcggatgag tttccagcgg cccgaaggca
     2461 ttaccctctt ccctgcatca gggcaccacc gcaacctgat cgaggccggt actttccgca
     2521 agcccgaaca tcaggttcat gttctggacc gcctgcccgg aagcgccctt aaccaggttg
     2581 tctatgaccg agagcacgac cagcgtgtcg gagcgtcgcg cgctctccgg cggccgatgc
     2641 acggcaagcc ggcaggtgtt cgcggcgcgc accgagcggg tgtcggggtg gctgcctgcc
     2701 ggcatgacat cgacgaacgg ctcgcccgcg tagcgcttct cgaaaagctg ctggaaatcc
     2761 atctcgcgca cgatgcgcgc gtagagtgtc gcgtgaatgc cgcggatgag cggtgtgaga
     2821 tgcggcgtga acacgagccc gacctcgcgc ttcgccgcct gcgcgagacc ctggcggatc
     2881 tcgggccagt ggcggtgtcc cgcgacgtga taggccgcga agttgtccga agcctcggag
     2941 aacatgagat tgagctcggt cttgcgtccc gcgccgctca cgccggactt ggcgtcggcg
     3001 atcaggtgct cgaggtccac cacgcccgcc tcgacaagcg gcaggaagcc gagctggacc
     3061 gcggtgggat agcagcccgg gttcgccacg agccgcgcgc ggcgtatctc ttcccgattg
     3121 agctcgggca gtccgtagac cgcttcgcga acgagctcgg gcgccgcgtg cttcgtcttg
     3181 taccagcgct cccactccgc cacatccttg atgcggaaat cggccgagag gtccaccacg
     3241 cgcgccccgc cttcgagcac cgcgcgcgct tctcccatgg caacgccgtt cggggtagcg
     3301 aagaacacca cgtcgcagcc ggcgagcgcg cgcgcgcttg gctcgctgaa ggcgagctcg
     3361 acgcgcccgc gcaggctcgc aaacatgcgc gcgaccggcg tgccggcttc cttgcgcgag
     3421 gtaatcgcct tgagctccac gccgggatgc tgcgcgagca ggcgtagcag ctcgacgccc
     3481 gtgtatcccg ttcctccgac aatgcctgct ttgatcataa aaaagcaaaa gccgccattc
     3541 ggcggctttc ggttcgcgga atccgtgtat tcagcgtttc gagtactgct tgcgccgcct
     3601 tgccttgtgc aggccgactt tctttcgctc cacttcccgc gcgtcgcgcg tcaccaaccc
     3661 ggccgcgctc aatgcgctct tcaggcccgc atcatactcg atcagcgccc gggcgatgcc
     3721 gtggcgcacc gcgcccgcct ggccgctctc gccgccgccg tagacattga ccatgatgtc
     3781 gaagctcgcg aggttcttgg tgagatcgag cggctggcgc accaccatgc gcccggtctc
     3841 gcggccgaag aactggtcga cgggcttgcc gttcaccttg aactcgccct tgccgggctt
     3901 gaggaacacg cgggccaccg cgctcttgcg ccgccccgtt ccgtagtaat agtcaccgac
     3961 catcgcagat ctccagtgct ttcggctgtt gcgccgaatg gggatgagtt gcgccgccgt
     4021 agattttgag cttgcgcagc atggcatagc cgagcgggcc cttgggcagc atccccttca
     4081 ccgcgatgtg cagtacgcgc tcgggtcgct tggcatgcag cttggcgaag ctggtctggc
     4141 gcagctgtcc cggctgcccg ctgtggcggt agtagatctt gcgctcggtc ttgcggccgg
     4201 tgacgcgaag cttcgcggcg ttcaccacga taatgtagtc gccggtgtcg atatgcggag
     4261 tgtattgcgg cttgtgcttg ccgcgcagcc gcaccgcgat ctgcgccgcg aggcggccga
     4321 gcaccttgtt ctgcgcgtcg accacgtacc agccctgcgg aacctcgcct gtttttgcgg
     4381 tgtaggttgt catgccggaa gcgagaaaag ctgcggattt tacacaaaaa agctagatta
     4441 gtgtcatgaa ctgcacggtg cgctggatgg gcgagggcat gagcttcatc gccgagaccg
     4501 gctcgaagca cctcgtcgcg atggacggcg cacccgacgg cggcggccgt aacctcgcgc
     4561 cccggcccat ggagatggtg ctcgccggga gtgggggctg cgcggcctac gacgtggtcc
     4621 ttatcctgcg gaaaagcggc caggacgtga ccggctgcga ggtgaagctc gcggccgagc
     4681 gcgcgagcag cgatccgaag gtcttcaccc gggtccacat gcatttcgtc gtgcggggcc
     4741 gcgcgctgaa gcgcaacctc gtcgagcacg cgatccggct gtcgcatgag aaatattgct
     4801 cggcgaccgc gatgctcgtg aagaccgccg aagtgacgcg cgattttgag atcatggaag
     4861 cctgatgcgc ccgatactcg cgctcgtcct cgcctgcagc gccgcgctcg ccgccgccca
     4921 gcttcgcacc atcccgaagg aagcgatccc cggcaagctg cgccacctgc aggcgatggt
     4981 ggtcgagatc gacggcaagc cgcgccagct cgcgcccggc gctcagatcc gcgacaccga
     5041 caaccggctc gtgctgcccg cctcgctcgt acagaagacc gtcgtcggat acacgctcga
     5101 cggcgcgggt tccgtgcacc gcgtctggat cctgtcgccg gccgagcgcg cccggctgcc
     5161 gaaga
//
LOCUS       DASUNT010000550         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1301259, whole genome shotgun sequence.
ACCESSION   DASUNT010000550 DASUNT010000000
VERSION     DASUNT010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301259"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFX94_11170"
     CDS             complement(<1..443)
                     /locus_tag="VFX94_11170"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5768785.1"
                     /translation="MTGIEFGPAKPADLPRLVELLGILFESEAEFSPNAEKQRAGLQA
                     ILADPAKGKIFVAREGREVVAMASLLYTVSTAEGGKAALFEDLVTAPEQRKRGIGEAL
                     LKYVVAQARAEGVLRITLLTDMQNERAQAMYRRAGFVGSPMKPMRL"
     gene            complement(440..1522)
                     /gene="ychF"
                     /locus_tag="VFX94_11175"
     CDS             complement(440..1522)
                     /gene="ychF"
                     /locus_tag="VFX94_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX5768786.1"
                     /translation="MKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGIVAV
                     PDTRLDVIAEIAQPQKVVPAVVEFVDIAGLVKGASEGQGLGNQFLAHIRETDAVAHVV
                     RCFEDPNVVHVAGSVDPVSDIEVINTELGLADLDSVDKQLAKYEKVAQTGGDKEAQRL
                     VSVLRKVRTTLDAGQPARATELSNEERAVLRPLFLLTMKPTMYVANIGERGSKELLAK
                     VQAHAAKEGAPVVPVSAALEAQIADMTDEDKKLFLEDMGMEEPGLNRVLRAAYKLLGL
                     QTFFTAGPKEARAWTVRAGATAPQAAGLVHTDFERGFIRAEVASYEDYVACGGEQGAK
                     EAGKLRLEGRDYRVRDGDVIHFRFNV"
     gene            complement(1519..2181)
                     /gene="pth"
                     /locus_tag="VFX94_11180"
     CDS             complement(1519..2181)
                     /gene="pth"
                     /locus_tag="VFX94_11180"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX5768787.1"
                     /translation="MIRLVVGLGNPGKEYERTRHNAGFWLVERFAQANGVVLRKDPKF
                     KALVGKHAETWLLLPQAFMNLSGQPVQMLAGFFKIKPEEVLVVHDELDFPPGTARLKQ
                     GGGIAGHNGLKDISQRLASHDYWRLRLGVGKPPAGTEGADYVLQKPPAEERTAIDGAI
                     DKAIALMPQIIAGDMQAAMNKLHTEEPHDPEPVKQPEVKKEAAEDPAAKPGLLKSLFG
                     RK"
BASE COUNT          336 a          763 c          695 g          429 t
ORIGIN      
        1 agccgcatcg gcttcatcgg cgagccgacg aatccggcgc gccggtacat cgcctgcgcc
       61 cgctcgttct gcatgtcggt gagaagcgtg atgcgcagca cgccctcggc gcgggcctga
      121 gcgacgacgt acttaaggag cgcctcgccg atgccgcgct tgcgctgctc gggcgcggtc
      181 acgagatcct cgaacagcgc cgccttgccg ccttccgcgg tactcaccgt atagagcagg
      241 ctcgccattg cgacgacttc gcgcccttcg cgcgcgacga agatcttgcc cttcgccgga
      301 tcggcaagga tcgcctgcag gccggcgcgc tgcttttcgg cgttaggaga aaattccgcc
      361 tcggactcga aaaggatgcc gagcagctcg acaagccgcg gcaggtcggc gggtttcgcc
      421 ggtccgaatt cgatgccggt cacacgttga acctgaagtg aattacgtcg ccatctcgca
      481 cgcggtagtc gcggccctcg agccgcagct tgcccgcctc cttcgccccc tgctcgccgc
      541 cgcaggcgac gtagtcttcg tacgacgcca cttcggcgcg gatgaagccg cgctcgaaat
      601 cggtgtggac cagacccgct gcctgcggtg cggtggcgcc ggcgcgcacg gtccacgcgc
      661 gcgcctcctt cggcccggcg gtgaagaatg tctgcaggcc gagcagcttg tacgccgcac
      721 gcaagacgcg gttcaacccc ggctcctcca tccccatgtc ctcgaggaaa agcttcttgt
      781 cctcgtcggt catgtcggcg atctgcgcct cgagcgccgc ggacacgggc accaccggcg
      841 cgccttcctt ggccgcgtgg gcctgcacct tcgccaggag ctcctttgag cctcgctcgc
      901 cgatgttggc gacgtacatc gtcggcttca tcgtcaggag aaaaagcggt cgcagtacgg
      961 cacgctcttc gttcgaaagc tcggttgcac gcgccggctg gcccgcatca agagtcgtgc
     1021 gtacttttct caggacggaa accaggcgtt gcgcctcctt gtctccgccc gtctgtgcga
     1081 ccttctcgta cttagcgagc tgcttgtcaa ccgagtcgag atccgcgagg ccgagctcgg
     1141 tattgatcac ctcgatgtcc gacaccgggt ccaccgagcc ggcgacatgc accacgttcg
     1201 gatcctcgaa gcagcgcacg acatgcgcga cggcgtcagt ctcgcggatg tgggcgagga
     1261 actgattgcc cagtccctgg ccttcggaag cgcctttcac cagcccggca atgtccacga
     1321 actccaccac agccggaacc actttctgcg gctgcgcgat ctcggcgatg acatcgagcc
     1381 gcgtgtcggg caccgccacg atgccgacgt tcggctcgat ggtgcaaaag gggtagttct
     1441 cggccgcgat cccggccttg gtcagcgcat tgaacagcgt ggacttgccg acgttgggca
     1501 ggcccacgat gccgcacttc atttcctgcc gaagagcgac ttgagcagac ccggcttggc
     1561 agccgggtct tctgcggctt ccttcttgac ttcgggttgc ttgacgggct cgggatcgtg
     1621 cggctcctcg gtatgcagct tgttcatcgc ggcctgcatg tcaccggcga tgatctgcgg
     1681 catgagggcg atcgccttgt cgatcgcgcc gtcgatcgcc gtgcgctcct cggccggagg
     1741 cttctgcagc acgtagtccg cgccctcggt gcctgcgggc ggcttgccca cgccgaggcg
     1801 caggcgccag tagtcgtgcg acgcgagccg ctgcgagatg tccttcaggc cgttgtgccc
     1861 ggcgatgcct ccgccctgct tgagccgcgc ggtgccgggc gggaaatcga gctcgtcgtg
     1921 caccacgagg acttcctcgg gcttgatctt gaagaatccg gcgagcatct ggaccggctg
     1981 tccgctgagg ttcatgaacg cttgcggcag cagcagccac gtttcggcgt gcttgccaac
     2041 gagcgccttg aacttcgggt ccttgcgcag cacgacgccg ttcgcctgcg cgaagcgctc
     2101 gaccagccag aagcccgcgt tgtggcgcgt gcgctcgtac tccttgcccg ggttccctaa
     2161 gccaacaaca agcctgatca cttcttctct tttttgtcag ccttgtcagc agcaggcttc
     2221 tcg
//
LOCUS       DASUNT010000551         2594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_686726, whole genome shotgun sequence.
ACCESSION   DASUNT010000551 DASUNT010000000
VERSION     DASUNT010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686726"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VFX94_11185"
     CDS             <1..407
                     /locus_tag="VFX94_11185"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX5768788.1"
                     /translation="KIKPGDVVVIRYEGPKGGPGMQEMLAPTSALIGQGLGESVGLLT
                     DGRFSGGTWGMVVGHVAPEAYVGGTIALVKEGDSITIDAKKRLLQLNVSAKELAARRK
                     KWKPPKPRYTQGLLAKYMKLVSTASRGAITDG"
     gene            complement(404..976)
                     /locus_tag="VFX94_11190"
     CDS             complement(404..976)
                     /locus_tag="VFX94_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEX5768789.1"
                     /translation="MKSLAPEVLHFWFGEGAEYGKRHKRWFEKNVEFDAEVAKRFGPL
                     HEDMLAGKHRDWLEARRPCLARIIVLDQFPRHIHRGTARAFSGDALALEAAKHLLGKG
                     WGRELLPVERMFAYLPFEHSESLEDQECACELCEALLAFPEVADTHRYALAHRDIIRR
                     FGRFPHRNAALGRASTPEEVEFLKQPGSGF"
     gene            1019..1378
                     /locus_tag="VFX94_11195"
     CDS             1019..1378
                     /locus_tag="VFX94_11195"
                     /inference="COORDINATES: protein motif:HMM:NF042483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HEX5768790.1"
                     /translation="MRRIIAALLAISLIVPLPARAAMLATDSALGGDRERIGQLLDRT
                     DVLARLQAYGVSPSEVKARVAALTDAEAAELAERIEALPAGGVGVLGAALIVFLVLLL
                     TDILGYTKVFPFTRPMK"
     gene            1375..>2594
                     /locus_tag="VFX94_11200"
     CDS             1375..>2594
                     /locus_tag="VFX94_11200"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX5768791.1"
                     /translation="MNRLAGETSPYLQQHAANAVDWYPWGPEALERARTEDKPILLSI
                     GYSACHWCHVMAHESFEDPGVAEVMNRHFVNVKVDREERPDLDQIYQSAHQMLRQRSG
                     GWPLTMFLSPDGTPFFGGTYFPKEPRHGLPGFAELCERVAAVWREKRADIAAQNAEVR
                     NALERTLPQPAARRDELHAQPVKALLDTLKANFDARLGGFGAAPKFPHPTDLELCLRK
                     GETQIALTTLERMCQGGIYDQLGGGFCRYSVDAEWLIPHFEKMLYDNGPLLGLLADAW
                     LVSGGALYKECAAATAGWIMREMQSPEGGYYSSLDADSEHEEGKFYLWSREEAQQLLT
                     EEEYAAFAARYGLDRPPNFENRHWHLYTANNAQSPVLESAKRKLFAAREKRVRPGRDE
                     KVLVSWNALAIRGMA"
BASE COUNT          457 a          850 c          862 g          425 t
ORIGIN      
        1 ggaagatcaa gcccggggat gtcgtcgtca tccgctacga aggcccgaaa ggcggtcccg
       61 gcatgcagga gatgcttgcg ccgacttccg cgctcatcgg ccagggcctg ggcgaatcgg
      121 tggggctgct gaccgacggg cgcttctccg gcggaacctg gggcatggtc gtcggccatg
      181 tcgcgcccga agcctatgtc ggcggcacga tcgccctggt gaaggagggc gactcgatca
      241 ccatcgacgc caagaaaagg ctgctgcagc tcaacgtttc ggcgaaggag ctcgccgcgc
      301 ggcgcaagaa atggaagccg ccgaagccgc gctacacgca gggcttgctc gccaagtaca
      361 tgaagctcgt atctaccgcg tcgcgcggcg cgataacgga cggctagaag cccgatcctg
      421 gctgtttcag gaactcgact tcttcaggcg tgctggctcg gccgagggcc gcattgcgat
      481 gcggaaaacg cccgaagcgc cggatgatgt cgcgatgggc aagggcgtag cggtgcgtgt
      541 cggccacctc ggggaaggcg agcagcgcct cgcatagctc gcacgcgcac tcctggtctt
      601 ccagcgactc gctgtgctcg aacggcaggt acgcgaacat ccgttccacc ggcagcagtt
      661 cgcggcccca ccctttgccg aggaggtgct tcgccgcctc gagcgcgagc gcgtcgcccg
      721 agaaggcgcg ggcggttccg cgatggatat gacgcgggaa ctggtcaagc acgataatgc
      781 gtgcgaggca gggccgtcga gcttcgagcc aatcccggtg cttgcccgcc agcatgtcct
      841 cgtgcaaggg accgaagcgc ttggcgacct cagcatcgaa ttcgacgttt ttctcgaacc
      901 agcgcttgtg gcgcttgccg tactcggcgc cttccccgaa ccagaagtgc aacacctcag
      961 gtgctaagct tttcatggac caaatccaac caagaacgga gacctcatga tcgctcagtt
     1021 gcgccgcatc atcgctgcct tgcttgccat tagcctcatc gtgccgctac ccgcgcgcgc
     1081 cgcgatgctc gcgaccgaca gcgccctggg tggggaccgg gaacgtatcg gacagttgct
     1141 cgatcgaaca gatgtgctgg cgcggctgca agcgtacgga gtaagcccct ccgaggttaa
     1201 ggcgcgcgtc gccgcgctga ctgacgccga ggcggccgag cttgccgagc gcatcgaggc
     1261 cttacccgcg ggcggggtag gcgtcctggg cgcggcgcta atcgtgttcc tcgtgctgct
     1321 gctgaccgat atcctcggat acaccaaggt ttttccgttc acgcgcccaa tgaagtgaac
     1381 cggctcgcgg gcgagacttc gccctacctg cagcagcacg cggcaaacgc cgtggactgg
     1441 tatccctggg gacccgaggc gctggagcgc gcccgtaccg aggacaagcc gatcctgctc
     1501 tcgatcggct actccgcgtg ccattggtgc catgtgatgg cgcatgagag cttcgaggac
     1561 cccggcgtcg ccgaggtgat gaaccggcac ttcgtcaacg tgaaggtgga ccgggaagag
     1621 cggcccgacc tcgaccagat ctaccagagc gcgcaccaga tgctcaggca gcgctccggc
     1681 ggctggccgc tcaccatgtt cctgtcgccc gacggcacgc cgtttttcgg cggcacctac
     1741 ttccccaagg agccgcgcca cggcctgccg gggtttgccg agctttgcga gcgcgtagcg
     1801 gcggtctggc gggagaagcg cgccgatatc gccgctcaga acgccgaggt gcgtaacgcg
     1861 ctcgagcgca cgttgccgca gcccgccgcg cggcgcgacg agctccacgc gcagccggtg
     1921 aaggcgctgc tcgatacgct gaaggccaat ttcgacgccc gcttgggcgg cttcggggca
     1981 gcgccgaagt ttccgcaccc caccgacctc gagctttgcc tgcgcaaggg cgagacgcag
     2041 atcgcgctga cgacgctcga gcgcatgtgc cagggcggaa tttacgatca gctcggcggc
     2101 ggcttttgcc gctacagcgt ggatgccgag tggctgatcc cccacttcga gaaaatgctc
     2161 tacgacaacg ggccgctact cggcctgctt gccgacgcct ggctcgtgag cggcggcgcg
     2221 ctctataagg agtgtgccgc cgcgactgct ggctggatca tgcgcgaaat gcagtcgccc
     2281 gaaggcggct actactcctc gctcgacgcc gacagcgagc acgaggaggg caagttctac
     2341 ctctggagcc gcgaggaagc tcagcagctt ctcactgagg aggaatatgc ggcgttcgcg
     2401 gcgcgctacg gccttgaccg gccgcccaac ttcgagaacc ggcattggca tctctacacc
     2461 gcgaacaatg cgcaaagccc ggtgctggag tcggcaaagc gcaagctttt cgccgcgcgc
     2521 gagaagcgcg tgcggccggg gcgcgacgaa aaggtgctcg tctcctggaa cgcgcttgcc
     2581 atccgcggca tggc
//
LOCUS       DASUNT010000552         2678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_73271, whole genome shotgun sequence.
ACCESSION   DASUNT010000552 DASUNT010000000
VERSION     DASUNT010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73271"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VFX94_11205"
     CDS             <1..510
                     /locus_tag="VFX94_11205"
                     /inference="COORDINATES: protein motif:HMM:NF023984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HEX5768792.1"
                     /translation="EVVGWRLRWADSAVVIRNRAPDSWFGAPGELYAEKRGPGVQELI
                     RRIEKAVHEYPYANTYSLWPGPNSNTFTAWIARAVPELETDLPATAIGKDYIGSQFFS
                     TAPSGSGFQFSLRGLFGVAASGVDGLELNLLSLNFGISPSGIKLPLVGRIGAARINGS
                     APAEQPAGL"
     gene            complement(533..1126)
                     /locus_tag="VFX94_11210"
     CDS             complement(533..1126)
                     /locus_tag="VFX94_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768793.1"
                     /translation="MKTAAIDSKAALVALLAALAAGCASVSKMESGEQTINGRLVVHL
                     EGAWNHVNAPGIGPAQTWTMEGLPIDQLRIYAGLKDGEVIHQPDNSGNRKNFSFRSSM
                     QPDDIVAMFEGMMTRDGSAFKLNKLEPMNFGGQKGFRFEFSLTRKVDGVELSGVGFGT
                     VANGELYAMLYAAPQLGFFARHQRGVEHMARSARLKL"
     gene            complement(1123..2262)
                     /locus_tag="VFX94_11215"
     CDS             complement(1123..2262)
                     /locus_tag="VFX94_11215"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5768794.1"
                     /translation="MRRRAFLSAGCACGAALLSRLAHAEEWAFPPRFPRPDVSSDEGG
                     LWAIMDREETRLRRSPFNVRDPKLRGYVQDIACRLGATHCLDIRVHLLRTPYFNASMA
                     PNGMMQVWSGLMLRVDNEAQLAAVLGHEIGHYLARHSVERLRDAKSRAAFGQFLGLFG
                     VVGALGQLGVLAGMLAYSRDQERDADRIGAMLMHKAGYDVSEASNVWANLLVETKARP
                     EGAPQSVLFATHPGAQERQASLAELAASYPGGEARREAWQELTAPFMREWLADEVRRG
                     EHEESIALLTRALGRGAQQSEYLYARGEIYRLRGKESDLELALADFRAAAAAGGEPPQ
                     MHRSLGFVHRARKDEPQAKASFQRYLELDPQAPDASMIKTYLEET"
     gene            complement(2259..>2678)
                     /locus_tag="VFX94_11220"
     CDS             complement(2259..>2678)
                     /locus_tag="VFX94_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768795.1"
                     /translation="DADELADINALHGAIARAISMHHFGPGFLNLPTKDGKLDWSLGE
                     GTRAIKKATGAEYALFTWVRDSYASGERIATMIALAVLGVGVGGGQQTGYASLVELET
                     GRVLWFNQLLRASGDLREAEKAAETIDTLLTNFPVPK"
BASE COUNT          404 a          919 c          885 g          470 t
ORIGIN      
        1 gaggtcgtcg gctggcgcct gcgctgggcg gacagcgcgg tggtgatccg caaccgggcg
       61 cccgactcgt ggttcggagc gcccggcgag ctctatgccg agaaacgcgg ccccggggtg
      121 caggagctca tccgccggat cgaaaaagcc gtgcacgaat acccgtacgc caacacgtac
      181 agtctctggc ccggcccgaa ctccaacacg ttcactgcct ggatcgcacg cgcggtgccg
      241 gagctcgaaa ccgacctgcc ggccacggcg atcggcaagg actacatcgg ctcgcagttt
      301 ttctccacgg cgccgagcgg cagcggattt cagttttctc tacgcgggct tttcggcgtg
      361 gcggcgagcg gggtggacgg cctggagctc aatctgctca gcctgaactt cggcatcagc
      421 ccctcgggga tcaagcttcc gcttgtcggg cgcatcggcg ccgcacgcat caacggctcg
      481 gctccggcgg agcagccggc cggcctctga gcggttccag gtcggtgagg gcttagagct
      541 tcagccgggc gctgcgcgcc atgtgctcca cgccgcgctg gtggcgcgcg aagaatccga
      601 gctgcggcgc ggcgtacagc atggcgtaga gctcgccgtt cgcgacggtg ccgaagccga
      661 ccccggaaag ctccacgcca tcgaccttgc gcgtgagcga gaactcgaag cggaatccct
      721 tctgtccgcc gaagttcatc ggctcgagct tgttcagctt gaacgccgag ccgtcgcggg
      781 tcatcatgcc ttcgaacatc gccacgatgt cgtcgggctg catgctcgag cggaagctga
      841 agttcttccg gttgcccgaa ttgtccggct ggtggattac ctcgccgtcc ttgaggccgg
      901 cgtagatccg caactggtcg atcggcagcc cctccatggt ccaggtctgc gccggtccta
      961 tgccgggggc gttcacatgg ttccatgccc cttccagatg gaccaccagc cgtccgttga
     1021 tcgtctgctc gcccgattcc atcttggaga ccgatgcgca tccggcagcc agagcggcga
     1081 ggagcgcgac cagagcggct ttcgagtcta ttgcggcggt tttcatgtct cctccagata
     1141 ggtcttgatc atcgaagcgt ccggcgcctg cgggtcgagc tcgaggtagc gctggaagct
     1201 cgctttagcc tgcggctcgt ccttgcgcgc gcgatgcacg aaccccaagc tgcggtgcat
     1261 ctgcggcggc tcgccaccgg ccgcggccgc cgccctgaaa tcggcgagcg caagctcgag
     1321 gtcgctttcc ttgccacgca gccggtagat ttcgccgcgc gcgtacagat actcgctttg
     1381 ctgtgctccg cgcccgagtg cccgcgtgag cagcgcgatg ctttcctcgt gttctccgcg
     1441 cctcacttcg tccgcgagcc attcgcgcat gaaaggagcc gtcaattcct gccaggcttc
     1501 ccgcctcgct tcgccgccgg ggtaggaggc ggcaagctcg gcaaggctcg cctggcgctc
     1561 ttgggcgccg gggtgcgtcg cgaacaggac actttgcggc gcgccttccg gtcgagcctt
     1621 cgtctccacc agcagattag cccacacgtt ggacgcctcg gacacgtcgt atccggcttt
     1681 atgcatgagc atcgcgccga tgcggtcggc gtcgcgctcc tggtcgcgcg aataggcgag
     1741 catgccggcg agcacgccga gctggccgag cgcgcccacg acgccgaaca agccgaggaa
     1801 ctgcccgaag gcggcgcgcg atttcgcgtc gcgcagacgc tcgacgctgt ggcgggcgag
     1861 gtaatggccg atctcgtgcc ccagcacggc ggcgagctgc gcttcgttgt cgacccggag
     1921 catgaggccg ctccacacct gcatcatccc gttcggcgcc atcgacgcgt tgaagtaggg
     1981 tgtgcgcaac agatgcacac ggatgtcgag gcagtgggtc gcgccgagcc ggcaggcgat
     2041 gtcctgcacg tagccgcgaa gctttgggtc gcgcacattg aacggactgc ggcgcagccg
     2101 cgtttcctcc cggtccatga ttgcccagag cccgccttcg tcgctcgaca cgtccgggcg
     2161 ggggaagcgg ggcgggaacg cccattcctc ggcgtgcgcg agcctggaga gcagcgccgc
     2221 cccgcacgcg cagcccgcgc tcaggaacgc gcgtctcctc acttcgggac cgggaagttc
     2281 gtgagcaggg tatcgatcgt ctcggcggcc ttctccgctt ctcgcagatc gccgcttgcg
     2341 cgcagcagct ggttgaacca cagtacccgc ccggtctcca gctcgacgag cgaggcatac
     2401 ccggtctgct ggccgccgcc cacgccgacg ccgaggacgg cgagcgcgat catcgtggcg
     2461 atgcgctcgc cgctcgcata gctgtcccga acccacgtga acagcgcgta ctcggcgccg
     2521 gtcgccttct tgatcgccct cgtgccttcg ccgagcgacc agtcgagctt accgtccttg
     2581 gtgggcaggt tgaggaaacc cggcccgaaa tggtgcatcg agatcgcgcg cgcgatcgcc
     2641 ccgtgcagcg cgttgatgtc ggcaagctcg tcggcgtc
//
LOCUS       DASUNT010000553         2431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_686878, whole genome shotgun sequence.
ACCESSION   DASUNT010000553 DASUNT010000000
VERSION     DASUNT010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686878"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VFX94_11225"
     CDS             complement(<1..577)
                     /locus_tag="VFX94_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX5768796.1"
                     /translation="MRTLEQERSTRASRAALTITALGIVYGDIGTSPLYAVKETFAPG
                     HGIPLNVANILGGLSAIFWVLIIVVSLKYVVLVMRADNRGEGGIMALIALASAAVRKR
                     PHWRFFILTVGLFGASLFFGEAVLTPAISVVSAVEGLEIGTSAFKPWVLPIATLVLVA
                     LFMLQRQGTAVVGAFFGPVCLLWFIALGAVGL"
     gene            636..2009
                     /gene="trkA"
                     /locus_tag="VFX94_11230"
     CDS             636..2009
                     /gene="trkA"
                     /locus_tag="VFX94_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEX5768797.1"
                     /translation="MKIVILGAGQVGSSVAEGLVSEQNDITVVDLDAQRLRALEDRLD
                     LRTVIGSAGHPSVLKQAGIEDADLLVAVTQSDDTNLVACRLAAKLFNVPRRIARLRST
                     DYLSNEKVIGEEGFDIDLVISPEQVLTDYIAKLVEFPEALQVLDFAVGKVSLVAVRAF
                     AGGPLVGRPLKELRAHMPQIDTRIVAIFRGDRSIIPDGDTVVQPGDEVFCLAASGHIR
                     EVLRELRRMDRPVKRVMIAGGGNIGLRLAFALQQDYSVRVIESNKRRCELLGARLGDT
                     LVLNGDATDEELLEDESIGEMDLFVAVTNDDENNIMGSLLAKRMGARRVVALINRRSY
                     VDLLHAGQIDIAISPAQATIGTLLAHVRRGDVVAVHSLRRGAAEAIEAVVHGGPESSR
                     IAGRRVGQIALPPGATIGALVRGEQVIMCHHDTGIEAGDHVIVFVSSKRLVPQVEKLF
                     QVSASFL"
     gene            2010..>2431
                     /locus_tag="VFX94_11235"
     CDS             2010..>2431
                     /locus_tag="VFX94_11235"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEX5768798.1"
                     /translation="MQALLPVANVLGALLMAFAVTYLLPIATSLIYDDGTAIAFVLAM
                     LIDFAVGCVLWLATRRHRRDLRPRDGYLLVTLGWLLMAAAASIPLVIALPGLSYTDIF
                     FETMSGLTTTGSTVIVALERLQPAVNLWRHALQWYGGMG"
BASE COUNT          406 a          830 c          815 g          380 t
ORIGIN      
        1 agagtcccac cgcgccgagc gcgatgaacc agagaaggca taccggcccg aagaacgcgc
       61 ccacgaccgc ggttccctgc cgctggagca tgaaaagcgc cacgagcacg agcgtcgcaa
      121 tgggcagcac ccacggcttg aaggcgctcg tgccgatctc caggccttcg accgcggaca
      181 cgaccgagat tgccggcgtg agcaccgcct cgccgaagaa aagcgacgcg ccgaacaatc
      241 ccacggtcag gatgaaaaag cgccagtgcg gacgcttgcg taccgcggcg ctcgcaagcg
      301 cgatgagcgc catgatgccg ccctctccgc ggttgtcggc gcgcatgacg agcacgacgt
      361 acttgagcga aaccacgatg atcagcaccc agaaaatcgc cgacaggccg ccgaggatat
      421 tggcgacatt gagcgggatg ccatggccgg gggcgaacgt ctctttcacc gcgtacaggg
      481 ggctcgtccc gatgtcgccg tagacgatgc cgagcgccgt gatggtgaga gccgcccggg
      541 aagctctggt tgagcgttct tgttccaggg tgcgcaaagc tactcccgca ctgcacaaag
      601 cgcaagcgag catcggttaa catccccgcc cagcgatgaa gatcgtcatc ctgggagcag
      661 gacaggttgg cagctcggtc gcggaaggcc tggtttccga gcagaacgac atcacggtcg
      721 tcgacctcga tgcgcagcgc ttgcgcgcgc tcgaggaccg gctggacctg cgcaccgtga
      781 tcggcagcgc ggggcacccg tcggtcctga agcaggccgg tatcgaggac gccgatctgc
      841 tcgttgccgt cacgcagtcc gacgacacca acctcgtggc ctgccggctg gcggcgaagc
      901 tcttcaacgt gccgcgccgc atagcgcggc tgcgctccac cgactacctc tccaacgaaa
      961 aagtgatcgg cgaggaaggc ttcgacatcg acctcgtcat ctcccccgag caggtgctca
     1021 cggactacat cgcgaagctg gtggagtttc ccgaagcgct gcaggtgctc gatttcgccg
     1081 tgggcaaggt gagcctggtc gcggtgcgcg cgttcgccgg cgggccgctc gtcggacgcc
     1141 cgctgaagga gctgcgcgcg cacatgccgc agatcgacac gcggatcgtc gcgatcttcc
     1201 gcggcgaccg ctcgatcatt cccgacggcg ataccgtggt gcagcccggc gacgaggtgt
     1261 tctgcctggc cgcctcgggc cacattcgcg aggtgctgcg cgagctgcgc cgcatggacc
     1321 ggccggtgaa gcgcgtgatg atcgccgggg gcggcaacat cggcctgcgc ctcgccttcg
     1381 cgctgcagca ggactactcg gtgcgggtca tcgagagcaa caagcgccgc tgcgagctgc
     1441 tcggcgcgcg cctgggcgat acgctggtgc tgaacggcga cgcgaccgac gaggagctac
     1501 tcgaggacga aagcatcggc gagatggacc tcttcgtggc ggtcacgaac gacgacgaga
     1561 acaacatcat gggctcgctg ctcgccaagc gcatgggcgc gcggcgggtg gttgcgctca
     1621 tcaaccggcg atcgtacgtg gatttgctgc atgcgggaca gatcgacatc gcgatctcgc
     1681 cggcgcaggc gacgatcggc acgctcctcg cgcacgtgcg ccgcggcgac gtggtcgcgg
     1741 tgcacagcct gcgccgcggc gcggccgagg cgatcgaggc ggtggtccac ggcggcccgg
     1801 aatcctcgcg catcgccggc cggcgcgtcg ggcaaatcgc gctgcccccg ggcgccacga
     1861 tcggcgcgct cgtgcgcggc gagcaggtca tcatgtgcca ccacgatacg ggcatcgagg
     1921 ccggcgacca cgtcatcgtg ttcgtaagct cgaagcgcct ggtgcctcag gtggagaagc
     1981 tcttccaggt gagcgcgtcg ttcctctaga tgcaggcgct cctgccggtc gccaacgtgc
     2041 tcggcgccct gctcatggcg ttcgccgtca cctacctgct gccgatcgcc acttcgctca
     2101 tctacgacga cggcaccgcg atcgccttcg tgctcgcgat gctgatcgac ttcgccgtcg
     2161 gctgcgtgct ctggctcgcc acccgccggc atcggcgcga cctgcgcccg cgcgacgggt
     2221 acctgctcgt cacgctgggc tggctcctga tggcggcggc ggccagcatt ccgctcgtca
     2281 tcgcgctgcc cggcctctcc tacaccgata tcttcttcga gacgatgtcg ggcctcacca
     2341 cgaccggctc gaccgtcatc gtggcgctcg agcgtctgca gcccgcggtc aacctttggc
     2401 gccatgcgct gcagtggtac ggcggcatgg g
//
LOCUS       DASUNT010000554         3597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_425195, whole genome shotgun sequence.
ACCESSION   DASUNT010000554 DASUNT010000000
VERSION     DASUNT010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425195"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VFX94_11240"
     CDS             <1..923
                     /locus_tag="VFX94_11240"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5768799.1"
                     /translation="LPQSVGILSRQDPTRATQLVERIQDLGARRLADMDASGIAKQVV
                     FVTAPGVQVFDAATAVSLASDLYDRLIREIGNFPHRYAALAAVAPQQPSKAAKELQRA
                     IGVGFKGAVINSHTQGEYLDEPKYWDIFEAAQALDMPLYLHPNTPPPAMIAPFLPRGL
                     DGAIYGFAVETGLHLLRIIVTGVFDRFPRLKIIVGHLGEGLPFWLFRLDFMHRSMVAS
                     KRYEGAGPLDHPPSHYLKNNVWVTTSGMQWAPAILFCQQVLGVDRVMYAMDYPYQFVP
                     DEVKVTDELPIADEDRRKLYQLNAERVFRL"
     gene            complement(949..2235)
                     /locus_tag="VFX94_11245"
     CDS             complement(949..2235)
                     /locus_tag="VFX94_11245"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX5768800.1"
                     /translation="MPSIPRRVAILGGVRIAFARANGAYMEHGNQDMLTAAMTALVER
                     FQLKGQRIGEVAAGAVIKHSRHWNLARECALGSGLHPDTPAYDLQRACGTSLSAVAQL
                     AHRIASGELDCAIAGGADSASDIPLSYSPKLQRTVLSLASGKTWGDKLSTIGSLRPRD
                     LAPKAPGIAEPRTGLSMGQHCEEMAKGWSVGRREQDEMALASHKNAAAAWQAGFFKDL
                     VVPFNDLTRDNNVRADTSLEKLASLRTAFDPSPAGTLTAGNSSPLTDGAAAALLSSED
                     WASSRKIPVQAYITFTETAAVDFVGMAGPKEGMLMAPAYAVPRMLERAGLKLQEFDIY
                     EIHEAFAAQVLCTLKAWESEEFCRKQLNRAPLGSIDRAKLNPKGSSVALGHPFAATGA
                     RIVATLAKQLAERGSGRGLISICAAGGMGVTAILER"
     gene            2324..>3597
                     /locus_tag="VFX94_11250"
     CDS             2324..>3597
                     /locus_tag="VFX94_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX5768801.1"
                     /translation="MDEALLLARVQFGLNIAFHILFPSITIGLAWLLVVFRLRYERSR
                     DPAWLEAYKLWVKVFALSFAMGVVSGITMSFQFGTNWPGYMNKVGNVAGPLLAYEVLT
                     AFFLEATFLGVMLFGMNRVSGRVHLASGVIVAAGTTLSAFWILALNSWMQTPAGHVVQ
                     DGVLIAGNWLHVIFNPSFPYRLVHMLLASAITAVFLVAGLSAWRLLKAATDASAGKTL
                     RFAVRLAALLVPAQVVVGDLHGLNTLEHQPAKIAAVEALWETQKGAPLTLFAIIDEDR
                     KRNDYAVEIPQALSVILTHDRDGVVLGLEEFGDDHPPVAPVFWSFRIMVGVGLAMLVL
                     SWTGLYILKRKEGSPPRSLLWVFAAFSFSGWIATLAGWMVTEIGRQPWLVTGILRTAD
                     AVGQASGAELGASLTAYVLTYAALLLSYMVVIT"
BASE COUNT          559 a         1186 c         1172 g          680 t
ORIGIN      
        1 ggcttccaca gtctgtgggg attctttctc ggcaagaccc cacgcgcgct acgcagctcg
       61 tcgagcgcat ccaggacctc ggggcgcgcc gcctcgccga catggacgcg agcggcatcg
      121 cgaagcaggt cgtgttcgta accgcgcctg gggtccaggt cttcgacgcc gctacggcgg
      181 tgtcgcttgc cagcgacctg tacgacaggt tgatcagaga aatcggcaac ttcccgcacc
      241 gctacgcggc actggccgcc gtcgcccccc agcagccttc caaagcggca aaagagctgc
      301 agcgcgcgat cggggttggc ttcaagggcg cggtgatcaa ctcgcacacc caaggcgaat
      361 atctcgacga gccgaagtac tgggacatct tcgaggcggc acaagcgctc gacatgccgc
      421 tgtacttgca cccgaacacg ccgccgccgg cgatgatcgc gccgttcctg ccgcgcgggc
      481 tcgacggcgc gatctacggc ttcgcggtcg agacggggct tcatctcctg cgcatcatcg
      541 tcaccggagt cttcgaccgc ttcccgcgcc tcaaaatcat cgtcggccac ctgggggaag
      601 gcctgccctt ctggctcttc cgcctggact tcatgcatcg gtcgatggtg gcatccaaaa
      661 gatacgaagg agcggggccg ctcgaccatc ctcccagcca ttacctcaag aacaacgtct
      721 gggtgacgac gagcggcatg cagtgggcgc cggcgatcct cttctgccag caggtgctcg
      781 gcgtcgaccg cgtgatgtac gcgatggact atccctacca gtttgtgccc gatgaggtga
      841 aagtcaccga cgagttgccg attgccgatg aggaccggcg caagctctac cagctcaacg
      901 ccgagcgggt gttccgactt tagaaaagga ggggcaggag ccttttccct aacgctcgag
      961 gatggcggtc acccccatcc cgccggcggc gcagatcgag atcaggccgc ggcccgaacc
     1021 gcgctcggcg agctgcttcg caagcgtggc gacgatgcgt gcgccggtcg cggcaaacgg
     1081 atggccgagc gcgacgctcg agcctttggg gttcagcttc gcgcggtcga tggaccccaa
     1141 tggcgcgcgg ttgagttgtt tcctgcagaa ctcttccgat tcccaggcct tgagcgtgca
     1201 caggacctgg gcggcgaagg cctcgtggat ctcgtagatg tcgaactcct ggagcttgag
     1261 cccggcgcgc tcgagcatgc gcggcaccgc gtatgccggc gccatcagca tgccttcctt
     1321 cggccccgcc atgccgacga agtccaccgc cgcggtttcg gtgaacgtga tgtaagcctg
     1381 cacaggaatt tttctcgaag aagcccagtc ctccgatgaa agcagcgcgg cggctgcgcc
     1441 gtcggtgagc ggcgagctgt tacccgcagt cagggtgccc gccgggctcg ggtcaaaagc
     1501 cgtcctgagc gaggccagct tctcgagcga ggtgtcggcg cgcacgttgt tgtcccgggt
     1561 gaggtcgttg aacggaacga cgaggtcctt gaagaaccct gcctgccacg ccgccgccgc
     1621 gttcttgtgg ctggcgagcg ccatctcgtc ttgctcccgg cgcccgaccg accacccctt
     1681 agccatctct tcgcagtgct ggcccatcga aaggccggtg cgcggctcgg cgatccccgg
     1741 cgccttgggc gcgaggtcgc gcgggcgcag gctgccgatc gtggagagct tgtcgcccca
     1801 ggtcttgccg ctcgcgagcg acaagaccgt gcgctgcagc ttcgggctgt aggagagcgg
     1861 gatgtcgctc gcgctgtcgg cgccgccggc gatggcgcag tcgagctcgc cggaggcgat
     1921 gcggtgggcg agctgcgcca ccgcggaaag actcgtgccg cacgcgcgct gcaaatcgta
     1981 ggccggcgtg tcagggtgga gcccgctgcc gagcgcgcat tcgcgcgcga ggttccagtg
     2041 gcgcgagtgc ttgatcactg cgcctgcggc tacttcgccg attctttggc ccttcagctg
     2101 aaaacgttca accagggccg tcatggcggc agtcagcatg tcctggttgc cgtgctccat
     2161 ataggcgccg ttggcgcgcg cgaacgcaat gcgcacgcca ccgaggatcg caacgcggcg
     2221 cggaatcgac ggcatgcgcg gactctaatg caagaaaggg tctcaccctc gattttcgct
     2281 ttccggtatg accgcggttt tggattttcg gtacattcgg gcgatggacg aggccctgct
     2341 gctggcgagg gtgcagttcg ggctgaacat cgccttccat atcctctttc cgagcatcac
     2401 gatcggcctc gcgtggctgc tcgtcgtttt ccgactgcgc tacgagcgca gccgcgaccc
     2461 cgcctggctg gaggcgtaca agctctgggt gaaggtattc gccctctcct tcgccatggg
     2521 ggtggtctcc ggcatcacca tgagtttcca gttcggcacc aactggccgg ggtacatgaa
     2581 caaggtgggt aacgtcgccg ggccgctgct cgcctacgag gtattgacgg cgttcttcct
     2641 ggaggcgacg tttcttggcg tgatgctctt cggcatgaac cgcgtgtcag ggcgtgtgca
     2701 cctcgcctcg ggagtgatcg ttgccgccgg caccaccctg tccgcgttct ggatcctcgc
     2761 gctcaactcg tggatgcaga ccccggccgg acacgtcgtt caggacggtg ttctcatcgc
     2821 cggaaactgg ctccacgtca tcttcaaccc ttcgtttccg taccggctcg tccacatgct
     2881 gctcgcttcg gcgatcaccg cggtgttcct ggtggcgggg ctctcggcct ggcggctgct
     2941 caaggcggcc accgacgctt cggcgggcaa gacgctgcga ttcgcggtgc ggctcgctgc
     3001 gcttctcgta ccggcgcagg tcgtggtggg cgacctccac gggttgaaca ccctcgagca
     3061 ccagccggcg aaaatcgccg ccgtcgaggc gctgtgggag acgcagaagg gggcgccgct
     3121 cacgctcttt gccattatcg acgaggaccg aaagcgtaac gactacgcgg tggagattcc
     3181 gcaggccctg tccgtcatcc tcacccacga tcgtgacggc gtcgtcctcg ggttggagga
     3241 gttcggcgac gatcacccgc cggtcgcgcc ggtgttctgg tcgttccgca tcatggtcgg
     3301 cgtcggcctg gcgatgctcg ttctctcctg gactgggctt tacatcctga aaagaaagga
     3361 aggaagccct ccgcgctcgc ttctctgggt ctttgccgcg ttcagcttct ccggctggat
     3421 cgcgacgctc gccggctgga tggtcaccga gatcggccgt cagccgtggc tcgtcaccgg
     3481 gatcctgcgc accgccgacg ccgtcggcca ggcgagtggc gccgagcttg gcgcaagcct
     3541 caccgcgtac gtgctcacct acgcggcgtt gcttctctct tacatggtcg tgatcac
//
LOCUS       DASUNT010000555         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1125446, whole genome shotgun sequence.
ACCESSION   DASUNT010000555 DASUNT010000000
VERSION     DASUNT010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125446"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VFX94_11255"
     CDS             complement(<1..384)
                     /locus_tag="VFX94_11255"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5768802.1"
                     /translation="MSSASAFGGAWARIRAISAKELRQLARDRLTFGMVVGIPLIQIL
                     IFGYSINFDVRHIEAAVVDEARTSGSRAFLQQLQASQVLDITRQVGSPAELQRAIASG
                     EVSVGIYIPPDYERRRQESGRPAAQI"
     gene            complement(381..1346)
                     /locus_tag="VFX94_11260"
     CDS             complement(381..1346)
                     /locus_tag="VFX94_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5768803.1"
                     /translation="MSSADPIAIRARGLTRRFGALTAVDHVDLDIPRAQIYGFLGPNG
                     SGKSTTIRMLCGLLRPSSGSVSVLGFDVARDSERLRRKLGYMTQKFSLWEDLTVAENL
                     EFIGQIYGLEKRERAARIGARLEEYRLTDRTGQRAGTMSGGQKQRLALAAATLHEPEL
                     LLLDEPTSAVDPQSRRDFWESLFRLVHRGTTILVSTHYMDEAERCHRLAILDTGRLVA
                     EGEPKELAANIDAAVVELETDDIEGARRALSTLEGVKSVAQLGVRLHVLMDRAVEHPE
                     RSVIDALRAAEVQARVERARASLEDVFVAATLAEPQGAPPARNVA"
     gene            complement(1343..2335)
                     /locus_tag="VFX94_11265"
     CDS             complement(1343..2335)
                     /locus_tag="VFX94_11265"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX5768804.1"
                     /translation="MRSAWIVVRMAATCALAAGCSGDADGPVALGTLERDRVELTAEA
                     HEPIVALNVQEGERVSADQVLLQLDSASLTKELASARANASSARHRLLELTRGPRMEE
                     ILEARARLEGAESDVDAETREYERLQGLVEQRLVSQSELDRQRASRDRATAERKRAQA
                     QLALLLKGTRIEELDQARDALSQAQSALERLEISVARLTVRAPRPGVIEALPYEVGER
                     PAAGAVVAVLQADGATYARVYVPAQLRTALRPGAAAHVSVEGIEEPLAGRVRYVSSEA
                     AFTPYYALTQEDRGRLSYLAEVTLTDEQAAARLPVGIPVEVTPDAQRATETASR"
     gene            complement(2328..>2540)
                     /locus_tag="VFX94_11270"
     CDS             complement(2328..>2540)
                     /locus_tag="VFX94_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768805.1"
                     /translation="ETERGHLRRDLDPALTVVSLISLALFPFVSLPITSRVLGVETNE
                     AGLEKLIAHTLRLFQRGVAAEGASHA"
BASE COUNT          351 a          928 c          872 g          389 t
ORIGIN      
        1 gatctgtgca gccggacgcc cgctctcctg gcgccggcgc tcgtagtccg gcgggatgta
       61 gatgccgacg ctcacctcgc cgctcgcgat cgcccgttgc agctccgcgg gggagcccac
      121 ctggcgcgtg atgtcgagca cctgcgaggc ctggagctgt tgcaggaagg cgcgcgatcc
      181 ggaagtgcgc gcctcgtcca ccaccgcggc ctcgatgtgg cgcacgtcga agttgatgct
      241 gtagccgaag atcaggatct ggatcagggg gatgcccacc accatgccga aggtgaggcg
      301 gtcgcgcgcc agctggcgca gctccttggc cgagatcgcg cggatgcgcg cccacgctcc
      361 cccgaacgcg ctggcggagc tcacgccacg ttgcgcgccg gcggcgcgcc ctgcggctcg
      421 gcgagcgtcg ccgcgacgaa aacgtcctcg aggctggcgc gagcgcgttc cacccgcgcc
      481 tgcacttctg ccgcgcgcag cgcatcgatc acgctgcgct ccggatgctc cacggcgcgg
      541 tccatgagga cgtgcagccg cacgccgagc tgggcgacac tcttcacgcc ctcgagggtc
      601 gagagcgccc gccgcgcgcc ttcgatgtcg tcggtctcca gctccacaac cgcggcatcg
      661 atgttcgccg cgagctcctt gggctcgcct tccgcgacca gcctgccggt gtccaggatg
      721 gccaggcggt ggcaccgctc ggcttcgtcc atgtagtggg tggagacgag gatcgtcgtg
      781 ccccgatgca ccaggcggaa caggctttcc cagaaatcgc gacggctctg cggatcgacg
      841 gcgctcgtgg gctcatcgag cagcagcaac tcgggctcgt gcagagtcgc cgccgcgagc
      901 gccaggcgct gcttctgacc gccgctcatc gtgccggcgc gttgcccggt ccggtccgtc
      961 agccggtact cctcgagccg cgcgccgatt cgagccgcgc gctcgcgctt ctcgagcccg
     1021 tagatctgtc cgatgaactc gagattctcc gcgaccgtga ggtcctccca cagcgagaac
     1081 ttctgcgtca tgtagccgag cttgcggcgc agtcgctccg aatcgcgggc cacgtcgaac
     1141 cccagcacgc tcacgctgcc cgagctcggc cgcagcagcc cgcagagcat gcggatggtg
     1201 gtggacttgc ccgagccgtt ggggccgagg aatccgtaga tctgcgcccg tggaatgtcc
     1261 aggtcgacgt gatcgaccgc ggtgagcgcg ccgaagcggc gcgtcagtcc gcgcgcgcgt
     1321 atggcgatgg gatcggccga actcatcgac tggcagtctc cgtggcgcgc tgcgcgtccg
     1381 gtgtaacctc gaccggtatg cccacgggca ggcgcgccgc tgcttgctca tcggtcagcg
     1441 tcacctcggc caggtagctg agacgtccgc gatcttcctg cgtgagtgcg tagtacggcg
     1501 tgaacgccgc ttcgctcgac acgtagcgca cacggccggc gagcggctcc tcgatgccct
     1561 cgacgctcac atgcgccgcg gcgccgggcc gcagcgcggt gcgcaactgc gccggcacgt
     1621 acacgcgcgc ataagtggct ccatccgcct gcagcaccgc aaccaccgca ccggccgcgg
     1681 gccgctcgcc gacctcgtag ggcaatgcct cgatgacacc cggccgtggc gcacgcacgg
     1741 tcagccgcgc gaccgagatc tccagccgct cgagtgccga ttgcgcctgg ctcagcgcgt
     1801 cgcgcgcctg gtccagctcc tcgatgcgcg tgcccttgag caggagcgcg agctgagcct
     1861 gggcccgctt gcgctcggcc gtggcgcgat cgcgggaggc gcgctgacga tccagctccg
     1921 actggctgac gagcctctgc tcgacgagcc cctgcaggcg ctcgtattcg cgcgtttcgg
     1981 cgtccacgtc gctctcggcg ccttccagcc gcgcgcgcgc ctccaggatc tcctccatac
     2041 gcgggccgcg cgtgagctcc agcagccggt ggcgcgccga gcttgcattg gcgcgcgcac
     2101 tcgcgagctc cttggtcagc gacgcggagt cgagctgcag cagcacctgg tccgcgctga
     2161 cgcgctcgcc ttcctgcacg ttgagcgcga cgatgggctc gtgcgcttcc gccgtgagct
     2221 ccacccggtc gcgctccagc gtgccgagcg ccacggggcc gtcggcgtcg cctgaacatc
     2281 ccgcggccag cgcgcaggtg gcggccatcc ggacgacgat ccacgcgcta cgcatgcgag
     2341 gcaccttccg cggcgacgcc gcgctgaaac agccgcaggg tgtgagcgat gagcttctcg
     2401 agtccggcct cattggtctc cacgcccagc acgcggctgg tgatcggcag cgacacgaac
     2461 gggaacaggg cgaggctgat gagcgagacg acggtgagcg ccgggtccag gtcgcgccgc
     2521 aggtggccgc gctcggtctc
//
LOCUS       DASUNT010000556         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1125509, whole genome shotgun sequence.
ACCESSION   DASUNT010000556 DASUNT010000000
VERSION     DASUNT010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125509"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2083
                     /gene="acnA"
                     /locus_tag="VFX94_11275"
     CDS             <1..2083
                     /gene="acnA"
                     /locus_tag="VFX94_11275"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX5768806.1"
                     /translation="QINLEYLARVVWTTERVAYPDSVLGMDSHTAMINSLGILGWGVG
                     GLEGGAGALGESVAMLVPEVIGCRLVGRPRPGVTSTDIVLTVTQTLRKHKVVDKFVEY
                     FGPGVAELSLPDRATIANMSPENGATMGFFPVDAETLRYLRLTGRDEAQVALVEAYCK
                     AQGLWRDAGTPPAEFTAVVEIDLSAVEPSVSGPSRPQDRVPLREAPAAFRSLVPEPKE
                     KDGDVAIAAITSCTNTSNPTVMIGAGLLARNAVARGLKVRPAIKTSLSPGSRVVADYL
                     AKSGLQDSLDALGFHLTGFGCMTCMGNSGPLPDEVAEAVEQGATAVAVLSGNRNFEGR
                     IHPALRANFLASPPLVVAYALAGSVLVDLEREPLGTGSDGKPVHLRELWPSGDEIHRV
                     IDEVLAPSLYRSRYATLLEGTTEWRALKGAAGETYAWSRGSTFIRRPPFFEDMEAKPA
                     EPADIRGARVLGLFGDMFTTDHISPIGVISKGTPAAQYLSSLGVAPADFVNYAARRLN
                     HDVMVRGTFANVRVRNEMTPDVEGSSTVHYPDGTRMPIHAAAERYRAEGVPLVVIAGA
                     EYGAGSSRDWAAKGTRLLGVRAVIAESFERIHRSNLVGMGVLPLQFPAGVTRKTLTLN
                     GDEVIDIVGISGAMAPRCELECTIARGDGNRHELKLLSRLDTRRDVDYFCHGGLLHHV
                     LRHRLSTEGSA"
BASE COUNT          336 a          733 c          747 g          325 t
ORIGIN      
        1 ccagatcaac ctggagtacc tcgcgcgcgt ggtgtggacg accgagcgcg tcgcttatcc
       61 cgactccgtg ctcggcatgg acagccacac cgcgatgatc aacagcctcg gcattctggg
      121 ctggggggtc ggggggctcg aaggcggcgc gggcgcgctc ggcgagtcgg tggcgatgct
      181 ggttcccgaa gtgatcggct gccggctggt gggaagaccg cggcccggcg tgacctccac
      241 cgacatcgtg ctcaccgtca cgcagacgct gcgcaagcac aaggtggtcg acaagttcgt
      301 cgagtacttc ggtcccgggg tcgccgagct gtcgctcccc gaccgcgcca cgatcgccaa
      361 catgagcccg gagaacggcg ccaccatggg ctttttcccg gtggacgccg agacgctgcg
      421 ctacctccgc ctcaccgggc gcgacgaagc gcaggtcgcg ctggtggagg cctattgcaa
      481 ggcacagggc ctgtggcgcg acgccggcac gccgcccgcc gagttcaccg ccgtggtcga
      541 gatcgacctc tcggcggtgg agccgagcgt ctcggggccg agccggccgc aggaccgcgt
      601 gccgttgcgc gaagcgccgg cggctttccg gagtctggtt cccgaaccga aagagaagga
      661 cggcgacgtc gccatcgcgg cgattacgag ctgcaccaat acgtcgaacc cgacggtgat
      721 gatcggcgcg ggcctgctgg cgcgcaacgc ggtggcgcgc gggctgaagg tgcgccccgc
      781 gatcaagacc tcgctctcgc ccggctcgcg cgtggtggcc gactacctgg cgaagagcgg
      841 actgcaggat tcgctcgacg cgctcggctt ccatctcaca ggattcggct gcatgacgtg
      901 catgggcaat tccgggccgc ttcccgacga ggtggccgag gcggtggagc agggcgcgac
      961 cgcggtcgcg gtgctctcgg gcaaccgcaa cttcgaaggg cgcatccatc ctgcgctgcg
     1021 cgcaaacttc ctcgcctcgc cgccgctcgt ggtggcgtac gcgctggcgg gcagcgtgct
     1081 cgtcgacctc gagcgcgagc cgctcggcac cggaagcgac ggcaagccgg tccatctgcg
     1141 cgagctctgg cccagcggcg acgagatcca ccgcgtgatc gacgaggtgc ttgcgccgtc
     1201 gctctatcgc agccgctatg cgacgctgct cgaaggcacg accgagtggc gcgccctgaa
     1261 aggcgcggcg ggcgaaacct acgcctggtc gcgcggcagc accttcatcc ggcgcccgcc
     1321 gttcttcgag gacatggagg cgaagcccgc ggagcccgcc gacatccgcg gcgcccgcgt
     1381 gctcggcctc tttggcgaca tgttcactac cgaccacatt tcgccgatcg gcgtaatctc
     1441 gaaagggacc ccggctgcgc agtacctttc atcgctcggc gtcgcgccgg cggacttcgt
     1501 aaattacgca gcgcggcgcc tgaaccacga tgtgatggtc cgcggcacgt tcgccaacgt
     1561 gcgcgtgcga aacgaaatga cgccggatgt agaagggagc agcaccgtgc attacccgga
     1621 cggcacgcgc atgccgatcc acgccgccgc cgagcgctac cgcgccgagg gcgtgccgct
     1681 ggtagtgatc gccggcgcgg agtacggcgc cggctcctcg cgcgactggg cggcgaaggg
     1741 cacgcggctg ctcggcgtgc gcgcggtgat cgccgagagc ttcgagcgca tccaccgctc
     1801 gaacctggtc ggcatgggcg tgctgccgct gcagttcccc gccggggtga cgcgcaaaac
     1861 gctcactctc aatggcgacg aggtcatcga catcgtcggc atttcaggag cgatggcgcc
     1921 gcgctgcgag ctcgagtgca cgattgcgcg cggcgacggc aaccgtcacg agttgaagct
     1981 gctctcgcgc ctcgatacgc ggcgcgacgt cgactatttt tgccacggcg gcctgctgca
     2041 ccatgtgctg cggcaccgcc tttcgaccga gggatcagca tgaatccaag gaaaaagctg
     2101 cgcgagctgc tcgccgcaac gcgctatgtc gtggtgcccg g
//
LOCUS       DASUNT010000557         2053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_73754, whole genome shotgun sequence.
ACCESSION   DASUNT010000557 DASUNT010000000
VERSION     DASUNT010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73754"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VFX94_11280"
     CDS             complement(<1..756)
                     /locus_tag="VFX94_11280"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5768807.1"
                     /translation="MTPQLEAFKDGLRQLGYLEGKNISLEFRSAEGRYESLRGLADEL
                     VALKVDLIVAFGTPPSQAAKAATRTIPIVMSAVGDPIGSGLVTSLARPGANVTGTSNL
                     SPPLVVKRLELLKESHPELRRVGLFANPANPAQAATVRAVEDAARSLKVEIFQYPVRS
                     TDAIRAAFSSMRTDRIEGLVLGNDTLLIGNSALIAQLAEKQRIRSSGNREFAEAGGLI
                     GYGSVADVNRLSASYVAKILKGAKPGELPIEQPS"
     gene            complement(1120..1290)
                     /locus_tag="VFX94_11285"
     CDS             complement(1120..1290)
                     /locus_tag="VFX94_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768808.1"
                     /translation="MTKTEHLQFLLLMIPSFLILVAAAVSMADLSLPEPSYGLYVATA
                     NAHAVAAENSYE"
     gene            1459..>2053
                     /locus_tag="VFX94_11290"
     CDS             1459..>2053
                     /locus_tag="VFX94_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5768809.1"
                     /translation="MKDRTTEVLPQALLDQIAAVATVRSFAKRAIIVTEGDETDSLYV
                     VLSGKARVFVADDKGREVQLNQLGPGEYFGEVTLDGGPRSASVMALDDTRCAIVKRAE
                     LTPFLEKHPELALHIVRKLAHRVRELTENVRSLALMDVYGRVARLLLELAEEKEGKLV
                     IGEALTHRDIAARVGASREMISRIFSDLAEGGYVRKED"
BASE COUNT          340 a          672 c          709 g          332 t
ORIGIN      
        1 gctcggctgc tcgatcggca gctcgccggg cttggcgcct ttcaggatct tggcgacata
       61 gcttgcagag agccgattca cgtccgcgac ggagccgtag ccgattagcc cgcccgcctc
      121 ggcgaactcg cggttgccgg aggagcggat gcgctgcttc tcagcgagct gcgcgatcag
      181 cgcggagttg ccgatcaaca gcgtgtcgtt gccgaggacc aagccttcga tgcgatccgt
      241 gcgcatggac gagaaggcag cgcggatcgc gtcggtgctg cgcaccgggt actgaaagat
      301 ctccaccttc agcgaccggg cggcgtcctc gacggcccgt accgtcgccg cctgcgcggg
      361 attcgctgga ttggcgaaca gcccgacgcg gcgcagctcc ggatgcgact ctttgaggag
      421 ctcgaggcgt ttcaccacca gcggcgggct gagattggac gtgcccgtga cattcgctcc
      481 cggacgcgcg aggctcgtca ccaggccgct gccgatagga tcgccgaccg cggacatgac
      541 gatcgggatc gtgcgggtcg cggccttcgc cgcctggctc ggtggcgtgc cgaaagccac
      601 gataaggtca accttcaggg cgacgagttc gtcggcgagc ccgcgcagcg attcgtagcg
      661 cccctcggcg gagcgaaact cgagcgaaat gttctttcct tcgaggtacc cgagctggcg
      721 cagtccatcc ttgaacgctt ccagctgcgg ggtcatgccg ccagcaaccc cgccgaagag
      781 gaggccgagc cgaggcagcc ttgctccttc cgcgacagcc aaacgcgccg gcacgcaagc
      841 tgcggcgagg gcggcgagaa ccgtccgccg cttcacgcga tgccccggtc ggcgcgcagg
      901 agcagcgccg gcggcacggt gaggccgagc gccttggcgg tcttcaggtt cactacgagc
      961 tccggccccc aagcggcggc gagggcggcg agcagccggc gccgattcga attcgtggga
     1021 cctccccccc cccccccaaa aaagaagact gccccgtcag cggggcagta tgaacctccc
     1081 tgctatttta ttcagcccgt cggcgccctg atctcgagcc tattcgtaac tgttctcggc
     1141 ggctacggcg tgggcgttcg ccgtggcgac gtacagcccg tacgagggct cggggaggga
     1201 caggtccgcc atggagacgg ccgcggcgac aaggatgagg aagctcggga tcatgagaag
     1261 caggaattgc aggtgctcgg tcttggtcat ggtgtgcctc ctcgttccag tggcgtcaga
     1321 ataggcttcc gcccggggcg ggtcagcgaa gcacggcgcc gcgttgtagt gatgtattta
     1381 ccccggtcga cgaactgggt cttgtcccgg ccgttgtgcg atcgcacaat gtccggtagg
     1441 tcacccgggg agacgtgatt gaaggaccgg acgacggaag tgctgcccca ggcgctgctc
     1501 gaccagatcg cagcagtcgc cacggtgcgc tcgtttgcca aacgggccat catcgtcacc
     1561 gaaggggacg agaccgattc gctctacgta gtgctctccg gcaaggcgcg cgtcttcgtc
     1621 gccgacgaca agggccggga ggtgcagctg aaccagctcg ggccgggcga gtacttcggc
     1681 gaggtgacgc tcgacggggg cccgcgctcg gcgtcggtga tggcgctcga cgacacccgc
     1741 tgcgccatcg tgaagcgggc cgagctcacg cctttcctcg agaagcaccc ggagctcgcg
     1801 ctgcacatcg tgcgcaagct cgcgcaccgc gtgcgcgagc tgaccgagaa cgtgcgcagc
     1861 ctagcgctca tggacgttta cgggcgtgtc gcccggctgc tcctcgagct tgccgaggag
     1921 aaggagggca agctcgtcat cggcgaggcg cttacccatc gcgacatcgc ggcgcgcgtc
     1981 ggcgcttcgc gcgagatgat cagccgcatc ttctccgacc tcgccgaggg cggttacgtg
     2041 cgcaaggaag acg
//
LOCUS       DASUNT010000558         2885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1037452, whole genome shotgun sequence.
ACCESSION   DASUNT010000558 DASUNT010000000
VERSION     DASUNT010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037452"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VFX94_11295"
     CDS             <1..825
                     /locus_tag="VFX94_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5768810.1"
                     /translation="REVFTAKVGRLMPTREDIKRAWPAVMRGTALGSFLGILPGGGAL
                     LASFGAYTVEKKVSSHPEQFGKGAIEGVAGPEAANNAGAQTSFIPMLTLGIPGNAVMA
                     LMIGALMIQGIAPGPQVMNEKPQLFWGLIASMWVGNAFLVILNLPLIGMWIKLLTVPY
                     RYLYPSILVFMSIGVFSLSNNPFDVLIMAIFGVLGYVCVKLECEPAPMILGFILGPLM
                     EENLRRAMLLSRGDPTTFITKPISAGFIIASVILLVIVALPALRKKREEVFVEQKT"
     gene            complement(827..1210)
                     /locus_tag="VFX94_11300"
     CDS             complement(827..1210)
                     /locus_tag="VFX94_11300"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5768811.1"
                     /translation="MADIRVYNVDALGKPLGQYSHVTRVAARELLFIAGMLAPGETFD
                     QQCSGVFAQIAQALKSGGAGWQNVVQFTTYLVHSQDIAKFMQWRLREFPRMFPDGRYP
                     PNTLLVVDRLVQEHFLVEVQAVAAL"
     gene            complement(1219..1629)
                     /locus_tag="VFX94_11305"
     CDS             complement(1219..1629)
                     /locus_tag="VFX94_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5768812.1"
                     /translation="MTDAEQVTTRHRILVVDDEESMRLIVAATLEKELGVEVQLAGTC
                     EQALRLAQTYAFDAILLDLMMPGIGGFAVLSAVRRSAPNARTPVVIVSARSDNAAMAR
                     CMSAGADAFVKKPLQLDELAQTVRAQFAARSERT"
     gene            complement(1822..2373)
                     /locus_tag="VFX94_11310"
     CDS             complement(1822..2373)
                     /locus_tag="VFX94_11310"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX5768813.1"
                     /translation="MGHRLSKIATRTGDGGDTGLGDGSRVPKDHLRVAALGEVDELNC
                     VLGLVLAEEVPEEMRGTLLQVQHDLLDLGGELSIPGHSLLKRERVEFLDSAIETLNST
                     LSPLKEFILPGGSRAAAAAHLARTVCRRAERRVVALGRKERVSELARQYLNRLSDLLF
                     IAARMLNQAAGVEDVQWRHQRKR"
     gene            complement(2357..>2885)
                     /locus_tag="VFX94_11315"
     CDS             complement(2357..>2885)
                     /locus_tag="VFX94_11315"
                     /inference="COORDINATES: protein motif:HMM:NF014640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX5768814.1"
                     /translation="TIPVVVGVGHETDFTIADFAADRRAPTPTAAAEMASPSRSELTA
                     RVARHAAFLHREMERKLRYAVQALDAWARRLVHPAQRLRSYQGQVIQLKARLAFAFSH
                     RGHRCQAHLARLEAALASLDPTAVLSRGYSITYDGAGGVVRSAAAVRPGERITTRLAE
                     GLIESEVRKNHGPSA"
BASE COUNT          452 a          987 c          964 g          482 t
ORIGIN      
        1 cgcgaggtgt tcaccgccaa ggtcgggcgc ttgatgccga cccgggagga catcaagcgc
       61 gcctggccgg cggtgatgcg tggcaccgcg ctcggctcgt tcctcggcat cctgcccggc
      121 ggcggcgcgc tgctcgcttc cttcggcgcc tacacggtgg agaaaaaggt ctcgagccat
      181 cccgagcagt tcggcaaggg cgcgatcgag ggcgtcgccg gtccggaagc ggcgaacaac
      241 gccggcgcgc agacctcgtt cattccgatg ctcacgctcg gcatcccggg caacgcggtg
      301 atggcgctga tgatcggcgc gctcatgatc cagggcatcg cgcccgggcc gcaggtgatg
      361 aacgagaagc cgcagctctt ctgggggctt atcgcgtcga tgtgggtcgg caacgctttc
      421 ctcgtgatcc tcaacctgcc gctcatcggc atgtggatca agctgcttac cgtaccctac
      481 cgctacctct acccgtcgat actcgtcttc atgtcgatcg gcgtgttcag cctgtcgaac
      541 aacccgttcg atgtgctgat catggcgatc ttcggcgtgc tcggctacgt gtgcgtcaag
      601 ctcgagtgcg agcccgcgcc gatgatcctc ggcttcatcc tcgggccgct catggaagag
      661 aacctgcgcc gcgcgatgct gctttcgcgc ggcgatccga cgaccttcat caccaagccg
      721 atcagcgcgg gcttcatcat cgcctcggtg atcctgctcg tgatcgtcgc gctgcccgcg
      781 ctgcgcaaga agcgcgaaga ggtgttcgtc gagcagaaga cctgagctag agcgcggcca
      841 cggcctgcac ttccaccaag aagtgctcct gcaccaaccg gtccaccacg agtagcgtgt
      901 tcggagggta ccgtccgtca ggaaacatcc gcggaaactc ccgcagccgc cattgcatga
      961 acttcgcgat gtcctgcgag tgcacgagat aggtggtgaa ctgcacgacg ttctgccagc
     1021 cggcgccgcc cgatttcagg gcctgcgcga tctgcgcaaa cacaccgctg cactgctggt
     1081 cgaacgtctc ccccggcgcc agcatgccgg cgatgaacag caactcgcgc gcggcgacgc
     1141 gcgtgacgtg agagtactgg ccgagcggct tgcccagcgc atcgacgttg tagacgcgga
     1201 tatctgccat ggaggacctc aggtgcgttc cgagcgggct gcaaattggg ccctgacggt
     1261 ctgcgccagc tcgtccagtt gcaggggctt cttcacgaag gcgtcggcgc cggccgacat
     1321 gcagcgcgcc atcgcggcat tgtccgagcg cgccgagacg atgacgaccg gggtgcgcgc
     1381 attcggcgcc gagcgtcgca ccgcggagag cacggcgaag ccgccgatgc cgggcatcat
     1441 gagatcgagc aggatcgcgt cgaaggcata ggtctgcgcg aggcgcagcg cctgctcgca
     1501 cgttccggcg agctggacct cgacgccaag ctccttctcc agcgtggccg cgacgatgag
     1561 gcgcatcgat tcttcgtcgt ccaccacgag gatgcggtgc cgcgtggtga cctgctctgc
     1621 gtccgtcacg cgccgtccat cgctcgtttt gcgcccgcca tggccagtac tttacacaag
     1681 gccccgtgta gaacacgggc ccggtcggtt gcacggtcgg tgagccgccg cccggctcga
     1741 gactcacggc ggagcgccgg cacgtcgtcg agcgacttgg ctgccgccac gagcttgagc
     1801 tccacttttt cgcccccgcc tctagcgctt gcgctggtgg cgccactgga cgtcttccac
     1861 ccccgccgcc tggttgagca tgcgtgccgc aatgaagaga aggtccgaca gccggttcag
     1921 gtactggcgc gcgagctcgc tcacgcgctc cttgcgccct agggcgacca cgcgccgctc
     1981 ggcccgccgg cacacggtgc gcgcgaggtg cgccgcggcg gcggcgcggc tgccgcccgg
     2041 caggatgaat tccttcaggg gtgagagcgt gctgttgagc gtctcaattg cgctgtcgag
     2101 aaactccacg cgctctcgct tgaggagcga gtgcccggga atgctgagct cgcccccgag
     2161 atcgagcagg tcgtgctgga cttggagcag cgtgccgcgc atctcctcgg ggacctcttc
     2221 ggcgagcacc aacccaagga cgcaattcag ctcatcgact tcgccgagcg ctgcgacgcg
     2281 aagatggtcc ttcggcacgc gcgagccgtc gccgaggccg gtgtcgccgc catcgccggt
     2341 tcgcgtggcg atcttgctaa gccgatggcc catgattctt cctgacttcg ctctcgatca
     2401 agccctcggc gagccgcgtg gtgatgcgct cgcccggccg cacggccgcc gcgctgcgca
     2461 ccacgccgcc cgcgccgtcg taggtgatgc tgtaaccgcg cgagagcacc gcggtcggat
     2521 cgagcgaagc gagtgcggcc tcgagccgcg cgaggtgcgc ctggcagcgg tgcccccggt
     2581 gagaaaacgc gaacgcaagc cgggccttga gctggatgac ctgaccctga tacgagcgca
     2641 gccgttgcgc cggatgcacc aggcgccgcg cccacgcgtc gagcgcctgc acggcgtagc
     2701 gcagcttgcg ctccatttcc cggtgcagga aagccgcgtg ccgcgccacg cgagccgtga
     2761 gctcggagcg cgaggggctc gccatttcgg cggcggcggt gggcgtgggc gcacgccggt
     2821 cggcggcaaa gtccgcgatg gtgaagtcgg tctcatgccc cacgccgacg accacgggaa
     2881 tagtg
//
LOCUS       DASUNT010000559         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_949632, whole genome shotgun sequence.
ACCESSION   DASUNT010000559 DASUNT010000000
VERSION     DASUNT010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949632"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFX94_11320"
     CDS             complement(<1..378)
                     /locus_tag="VFX94_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5768815.1"
                     /translation="MVLLCAGCHGMSGEGRIDAGYPRLAGQPHRYLERQLEAYADGRR
                     KSAVMEPLARSLTAGERRRLAAHFSSRTADAAPGDSYSKRGETLAVHGDEALRVQACQ
                     NCHGPMGRGQPPSGPALAGLGADY"
     gene            complement(380..2137)
                     /locus_tag="VFX94_11325"
     CDS             complement(380..2137)
                     /locus_tag="VFX94_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX5768816.1"
                     /translation="MTTSLKEVDAVLLGVGLVGSIIGRELVRAGLDVVGLERGEPRHT
                     VPDFQGPHMHDELRYAVRKSLMMNTSKETYTFRNHAAQTALPLRRLEGFLPGTGLGGS
                     AVHWNGQTWRFQDSDFRMRSHHLERYGRGMLPEEAAVQDWGVSYADMEPYFDRFEYLM
                     GICGKAGNLRGQKIAGGNVFEDPRSREYPNPPLKEPYGSSLFRAAAAKLGYHPFPMPS
                     ANMSRPYVNPEGLTLKTCTFCGFCERFGCEHFAKASPQTALLPVLLADKRFALRTGCQ
                     VTRINWSGREARSVTYVDEAGREFEQPARLVIVGLYALNNVRMLLLSKIGTPYDPKSG
                     RGVVGRNYAYQTTSGANVFYGSDQHINPFMRSGANGVVIADFVADNFDHAKLGFVGGG
                     YIGEVMSHGRPIHFQPMPPGTPKWGSAWKRALVKHYNHTVSIGVHGSSIAVRSNYLDL
                     DPTYRDAWDLPLLRITFDFPENDIRLSAYLTEKARGIAEAMGGGQVSASPRKRPYTAF
                     EYQSTHNTGGAIMGADRKTSVVNPWLQSWDLHNVFVVGASAFPQNASYNPTGPVGALA
                     YRAADAIVNRYLRKPGPLA"
     gene            complement(2121..>2356)
                     /locus_tag="VFX94_11330"
     CDS             complement(2121..>2356)
                     /locus_tag="VFX94_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEX5768817.1"
                     /translation="DLGAAGQGEFFELLLALTMEGYLGDPVYGGNRGMAVWKMIGFPG
                     AYANYYALVGKNVAFEAEPKSLAEGAHAHDHKP"
BASE COUNT          372 a          787 c          809 g          388 t
ORIGIN      
        1 atagtcggcg cccaggccgg cgagcgccgg gccgctcggc ggctggccgc ggcccatcgg
       61 tccgtggcag ttctggcatg cctgcacgcg caacgcctcg tcgccgtgca cggcgagcgt
      121 ctcgccgcgc ttggaatagg aatctcccgg cgccgcgtct gcagtacggg aggagaaatg
      181 cgcggcgagc ctgcggcgct cacccgcggt gagcgagcgc gcgaggggct ccatgaccgc
      241 gctcttgcgc cggccatcgg cataggcctc aagctggcgc tcgagatagc ggtgcggctg
      301 gccggcgagg cgcggatagc cggcgtcgat gcggccctca ccgctcatgc cgtggcagcc
      361 cgcgcaaaga agcaccatct caagcaagcg ggccgggctt gcggagatac cggttgacga
      421 tcgcgtcggc cgcgcgatac gcgagcgcgc cgaccgggcc ggtggggttg taggacgcgt
      481 tctgcgggaa ggccgaggcg ccgaccacga acacgttgtg caggtcccag gactgcagcc
      541 acggattcac cacgctcgtc ttgcgatcgg cgcccatgat cgcgccgccg gtgttgtgcg
      601 tggactggta ctcgaaggcg gtgtacgggc gcttgcgcgg cgaggccgac acttgcccgc
      661 cgcccatcgc ctcggcgatg ccgcgcgcct tttccgtgag gtacgccgag agccggatat
      721 cgttctcggg aaaatcgaac gtgatgcgaa ggagcggcag gtcccacgcg tcgcggtagg
      781 tcgggtcgag atcgagatag ttcgagcgca ccgcgatcga gctaccgtgc acgccgatgc
      841 tcacggtatg gttgtagtgc ttcaccagcg cacgcttcca tgccgagccc cacttgggcg
      901 tcccgggcgg catgggctgg aaatggatcg ggcggccgtg actcatcacc tcgccgatgt
      961 agccgccgcc gacgaagccg agcttcgcat ggtcgaagtt gtcggcgacg aaatcggcga
     1021 tcaccacgcc gttcgcgccc gagcgcatga acgggttgat gtgctgatcg cttccgtaga
     1081 acacgttcgc gccactcgtg gtctggtagg cgtagttgcg ccccaccacg ccgcggccgc
     1141 tcttcgggtc gtaaggcgtg ccgattttcg agagaagtag catgcgcacg ttgttgagcg
     1201 cgtacaggcc gacgatcacc agccttgccg gctgctcgaa ctcgcgcccc gcctcgtcga
     1261 cgtaggtgac gctgcgcgcc tcgcgcccgc tccagttgat tctcgtcacc tggcagccgg
     1321 tgcgcagcgc gaagcgcttg tccgcgagca gcacgggcag cagcgccgtc tgcggcgagg
     1381 ccttggcgaa atgctcgcag ccgaagcgct cgcagaatcc gcagaaggtg caggttttga
     1441 gcgtgagtcc ctcgggattc acgtagggcc ggctcatgtt cgcagaaggc atcggaaacg
     1501 ggtgatagcc gagcttcgcc gccgcggcgc gaaaaagcga cgagccgtag ggctccttca
     1561 ggggcgggtt cggatactcg cgcgagcgtg gatcctcgaa cacgttgccg ccggcgattt
     1621 tctgcccgcg caggttgccc gccttgccgc aaattcccat caggtactcg aagcggtcga
     1681 agtacggctc catgtcggcg taactcacgc cccagtcctg caccgccgct tcctcgggca
     1741 gcatgccgcg tccgtagcgc tcgagatggt gcgagcgcat gcgaaagtcc gaatcctgga
     1801 agcgccaggt ctggccgttc cagtgcaccg ccgagccgcc gaggccggtg ccgggcagga
     1861 agccttcgag gcgccgcagc ggcagcgcgg tctgggctgc gtggttgcgg aaggtgtagg
     1921 tctccttcga cgtgttcatc atgagcgact tgcgcaccgc gtagcgcagc tcgtcgtgca
     1981 tgtgcggccc ctggaagtcg ggcaccgtgt ggcgcggctc gccgcgctcg aggcccacca
     2041 cgtccagccc cgcgcgcacg agctcgcgcc cgatgatcga gcccaccagc cccacgccga
     2101 gcaggacggc gtcgacttcc ttaaggcttg tggtcatgcg cgtgcgcgcc ttcggcaagg
     2161 ctcttcggct cggcctcgaa cgcaacgttc ttgcctacga gcgcgtaata gttcgcgtag
     2221 gcgcccggaa agccgatcat cttccacacc gccatgccgc ggttgccgcc gtataccgga
     2281 tcgcctagat agccctccat ggtgagcgcg aggagcagct cgaagaactc gccctgcccg
     2341 gccgcgccga gatcga
//
LOCUS       DASUNT010000560         6060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1302121, whole genome shotgun sequence.
ACCESSION   DASUNT010000560 DASUNT010000000
VERSION     DASUNT010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302121"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VFX94_11335"
     CDS             <1..424
                     /locus_tag="VFX94_11335"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HEX5768818.1"
                     /translation="VTFSGEVRKGKQRFTMKVVVPVTSLCPCSKKISERGAHNQRSHV
                     TVTARTNEFVWIEEIIDLVEKQASSELYGLLKRPDEKFVTERAYDNPKFVEDMVRDVA
                     AVLNLDERIDAYVVESENFESIHNHSAYALIERNKRAR"
     gene            442..1128
                     /locus_tag="VFX94_11340"
     CDS             442..1128
                     /locus_tag="VFX94_11340"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX5768819.1"
                     /translation="MLGLTADAQDYASQRARMVAEVDAMYTETRAETGLSAMSAAVRN
                     ALGKVERHRLVPPGEASRAYRNHPLPIGAGQTISQPYIVALSTDLLNPTPADVVLEVG
                     TGSGYQAAVLAEIVSRVYSIEIIPALGSEARKRLEELDYRNIEVRIGDGYAGWPEKAP
                     FDGIVVTAAAPRVPQALVDQLKPGGRMVIPVGASGDIQYLKLLTKRADGGVDEKRVLP
                     VRFVPLVPGK"
     gene            complement(1184..1816)
                     /locus_tag="VFX94_11345"
     CDS             complement(1184..1816)
                     /locus_tag="VFX94_11345"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEX5768820.1"
                     /translation="MRPAILLLLFVLSTGVGAADVALIGLIGDKAAVLAVDGGDPKTV
                     KVGQKWHGITVISVERGEATVEIDGQRRVLKIGQHHRAAAQAADRQSITLAADSRGHF
                     ITQGAINGNPVRFLVDTGATVVALPAAEAMRLGIDYRKGQPGMSSTAGGMVSTYRVRF
                     DSVRLGDIEVLGVDGVVLEQGLDIALLGMSFLNRVEMKRDGHTMVLIRRF"
     gene            1815..2564
                     /locus_tag="VFX94_11350"
     CDS             1815..2564
                     /locus_tag="VFX94_11350"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEX5768821.1"
                     /translation="MKPVGIVRTARTEGPGYFATYLERRSIPWELVALDQGEKVPANA
                     RAYSGLAVMGGPMSANDDLPWIAPLLELLRNAVRADVPLIGHCLGGQLMSRAFGGAVR
                     VNPVREIGWGEVRVADNGVARDWLGELQAFETFQWHGETFSIPPGATRVLEGSHCLNQ
                     AFALGKHFAMQCHVEMTPELVRTWVGTGGEEMRTHRKSPAVQSAAEIEQDLEPRLERL
                     HQIADRIYDRWSEALSRGQTGEVTPRFTRGS"
     gene            complement(2548..3033)
                     /locus_tag="VFX94_11355"
     CDS             complement(2548..3033)
                     /locus_tag="VFX94_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX5768822.1"
                     /translation="MPSFDIVSQVDRQEVRNAVEQTNKEIANRFDFKGSDARVEQTDL
                     ALSVYADDEFKLGQVLDVLRQRLAKRNVDVRSLEPGAVEKISGDKVKRPVSVKVGVPQ
                     EKAKQIQKLIKDSKLKVAASIQGDAVRVSGTKKDDLQAAIQLVRQSVTDLPLQYINFR
                     E"
     gene            complement(3036..3692)
                     /locus_tag="VFX94_11360"
     CDS             complement(3036..3692)
                     /locus_tag="VFX94_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768823.1"
                     /translation="MRIQVLAGFFVASNVFAAPFAVQVGEARIGLDAPPGFADTSFTG
                     SPRLQELGESLTSASNRVLLFAISDADLRRFTLGDQLELRRYMVVYTPRASERERMTP
                     GSFKTFAAESLRDVGQMPAPADYRKYLESRPAGTVNLLAELRKDPDAVAVLLGARSKP
                     TGFFERSKYQLSSTSIVLLRGKPLTLSIYTQYDDDADVEWVRTTTARWIDELKRLNAR
                     "
     gene            3757..5106
                     /gene="argG"
                     /locus_tag="VFX94_11365"
     CDS             3757..5106
                     /gene="argG"
                     /locus_tag="VFX94_11365"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX5768824.1"
                     /translation="MAKILSKLPRGEKIGVAYSGGLDTCAAVRWMRANGAIPYTYSAN
                     LGQPDEADFEDIPRKALAYGAEKARLVECRPQLVAEGIAAIQCGAFHISTGGTTYFNT
                     TPLGRAVTGTMLVVAMREDGVDVWSDGSTYKGNDIERFYRYGLLTNPALRIYKPWLDQ
                     RFIDELGGRKEMSEYLSRAGVAYRTSAEKAYSTDSNILGATHEAKDLEFLNKAMAIVT
                     PIMGVAFWREDVPVKSEKVAVRFEQGQPVALNGQQFRNSVELLQEANAIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYRANGRRLGRLLYQG
                     RWFDPQAMMLRETAQRWVAAAVSGEVTLELRRGNDYSILDTTSPNLTYKPERLTMEKG
                     EEVFFAPADRIGQLTMRNLDIADTRDKLRIYGETGLLGEPKEGAVPLLGGGEPKKKA"
     gene            complement(5110..6012)
                     /gene="argF"
                     /locus_tag="VFX94_11370"
     CDS             complement(5110..6012)
                     /gene="argF"
                     /locus_tag="VFX94_11370"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX5768825.1"
                     /translation="MAVRHILQLRDLSLDELEHVFARTRWIKDKFKRYERYWPLEDRT
                     LAMIFEKPSTRTRMSFEAGMHQLGGAAIYLYTRDSQLGRGEPVEDAAQVISRMCDVIM
                     VRTFEQAIIERFAGASRVPVINGLTNEYHPCQILADVFTYVEHRGPIRGRTVAWIGDS
                     NNVCNTWLQAAELFDFRLNVSTPPGYEVEKERMNRNSEAFADPMAAARGADLVTTDVW
                     TSMGFEDENEERRRDFQDWQVDADMMRAAKKDALFMHCLPAHRGEEVAAAVIDGPQSV
                     VWDEAENRLHTQKALLEFLVLGKK"
BASE COUNT         1035 a         2006 c         2025 g          994 t
ORIGIN      
        1 agtgacgttt tccggcgaag tgcgcaaggg gaagcagagg ttcacgatga aagtcgtggt
       61 gccggtgacc agcctctgcc cctgctccaa gaaaatctcg gagcgcggcg cacacaacca
      121 gcgctcgcat gtcaccgtga ccgcgcgcac caacgaattc gtttggatcg aggagatcat
      181 cgacctggtg gagaagcagg cgtcgagcga gctgtacggc ctgctcaagc gcccggacga
      241 aaaattcgtc accgagcgcg cctacgacaa tcccaagttc gtcgaggaca tggtgcgcga
      301 tgtggcggcg gtgctcaacc tggacgagcg catcgacgcc tacgtggtcg agtcggagaa
      361 cttcgagtcg atccacaacc acagcgccta cgccctcatc gagaggaaca aacgcgcgcg
      421 ctagcggggt tgttcctcct catgctcggg ctcacggcgg acgctcagga ctatgcaagc
      481 cagcgggccc gcatggtggc ggaagtggac gcgatgtaca cggagacgcg cgcggaaacc
      541 gggctgtccg cgatgtcggc ggcggtgcgt aacgccctgg gcaaggtgga gcgccaccgg
      601 ctcgtgccgc ccggcgaagc ttcgcgtgcc taccgcaacc acccgctgcc gatcggcgcc
      661 ggccagacga tctcgcagcc ctatatcgtt gcgctttcga ccgatctcct gaaccccacg
      721 cccgccgacg tagtcctcga ggtcggcacc ggctcgggct accaggcggc cgtgctcgcc
      781 gagatcgttt ctcgggtgta ctcgatcgag atcatccccg cactcggcag cgaggcgcgc
      841 aagcgcctgg aagaactcga ttaccgcaac atcgaagtcc ggatcggaga cggatacgcc
      901 ggctggccgg agaaggcgcc tttcgacggc atcgtcgtca ccgcggcggc gccgcgcgtg
      961 ccgcaggctc tggtggacca gttgaagccc ggcggccgca tggtgattcc ggtgggcgct
     1021 tcgggcgaca ttcagtacct gaagctcttg accaaacggg ccgatggcgg ggtagacgag
     1081 aagcgcgtgc tgcccgttcg cttcgtgccc ctggtgccgg gaaaatagcc ggggtcagac
     1141 cccgatttct cggttggggt ccgaccctcg atctttcgtt tcgctagaac cggcggatga
     1201 gcaccatggt gtgcccgtcg cgcttcatct cgacgcgatt gaggaagctc atgccgagaa
     1261 gcgcgatgtc gaggccctgc tcgaggacca cgccgtccac tcccaaaacc tcgatgtcgc
     1321 cgagccgcac gctgtcgaat ctcacgcggt aggtcgacac catgccgccg gcggtgctgc
     1381 tcatcccggg ctggcccttg cggtaatcga tgccgagccg catggcttcc gccgcgggca
     1441 gcgcgacgac cgtcgcgccg gtatcgacga gaaatcgcac cgggttgcca ttgatggcgc
     1501 cttgggtgat gaagtgtccg cgcgagtcgg cggcgagcgt gatgctctgc cggtccgcgg
     1561 cctgggcggc cgcgcggtga tgctgcccga ttttcagcac gcggcgctgg ccatcgatct
     1621 ccaccgtcgc ctcgccgcgc tcgaccgaga tcacggtgat gccgtgccat ttctgcccga
     1681 ccttcaccgt cttggggtcg ccgccgtcca cggcgagcac cgccgccttg tccccgatga
     1741 ggccgatcag ggccacatcg gcggcgccca cgccggtgga caaaacgaaa agcagaagca
     1801 aaatagcggg tcgcatgaag cccgtcggta ttgtcaggac tgcgcgcacc gaaggtccag
     1861 gttattttgc cacctacctc gaacgccgct cgatcccgtg ggagctcgtc gcgctcgacc
     1921 aaggtgagaa agttcctgcg aacgcgcgcg cgtactcggg tctcgcggtc atgggcggcc
     1981 cgatgagcgc gaacgacgac ctgccgtgga tcgctccgct gctcgagctc ttgcgcaatg
     2041 ccgtgcgggc cgacgtgccg ctcatcgggc actgtctcgg cggccagctc atgtcccgcg
     2101 cgttcggcgg cgcggttcgc gtcaaccccg tgcgcgagat cggctggggc gaggtgcgcg
     2161 tcgccgacaa cggcgtcgca cgcgattggc tgggagagct tcaggccttc gagaccttcc
     2221 agtggcacgg cgagactttc agcatcccgc cgggcgcgac gcgggtgctc gaaggcagcc
     2281 attgcctgaa ccaggcgttc gcgctcggca agcacttcgc catgcaatgc cacgtcgaga
     2341 tgacgccgga gctggtgcgc acctgggtcg gcaccggcgg cgaggaaatg cgcacccatc
     2401 gcaagagccc cgccgtgcag tcggccgccg agatcgagca ggacctcgag ccgcgcctgg
     2461 agcgcctgca ccagatcgcg gaccgcatct acgaccgctg gagcgaagcg cttagccggg
     2521 ggcaaacggg ggaggttacc ccccgtttca ctcgcggaag ttgatgtact ggagcgggag
     2581 gtccgtcacg gattgccgga caagctgaat ggcggcctgc aggtcatctt tcttggtgcc
     2641 cgacacgcgc accgcgtcac cctggatcga ggcggcgacc ttgagctttg aatccttgat
     2701 cagtttctgg atctgcttcg ccttctcctg cggcacgccg accttgacgc tcaccgggcg
     2761 cttcaccttg tcaccggaga tcttctcgac cgcgcccggc tccagcgagc gcacgtcgac
     2821 gttgcgcttg gcgaggcgct ggcgcagcac gtcgagcacc tggccgagct tgaactcgtc
     2881 gtcggcatag acgctgagcg cgaggtccgt ctgctccacg cgggcgtcgg atcccttgaa
     2941 gtcgaagcga ttcgcgattt ccttgttggt ctgctccacc gcgttcctca cttcctggcg
     3001 atcgacctgc gagacgatgt cgaaggaggg catggtcagc gcgcgttgag acgcttcagc
     3061 tcgtcgatcc accgtgccgt cgtggtgcgg acccactcca cgtcggcgtc gtcgtcgtac
     3121 tgggtataaa tcgagagcgt gagcggcttg ccgcgcagca ggacgatgct ggtggacgaa
     3181 agctggtact tcgagcgctc gaagaagccg gtgggcttgc tgcgcgctcc gaggagcacg
     3241 gcgacggcgt cggggtcctt gcgcagctcg gcgagcaggt tcaccgtccc tgccgggcgc
     3301 gactcgaggt acttgcgata gtcggccggc gccggcatct gaccgacatc gcgcagcgac
     3361 tcggcggcga aggtcttgaa gctgcccggc gtcatgcgct cgcgctcgct ggcgcgcggg
     3421 gtgtacacga ccatgtagcg gcgtaactcc agctgatcgc cgagggtaaa gcgccgcaaa
     3481 tccgcatcgc tgatcgcgaa gaggagcacg cggttcgacg ccgaggtgag cgattcgccg
     3541 agctcctgca gccgcggcga gccggtgaag gaggtatcgg cgaagcccgg cggagcgtcc
     3601 aggccgatgc gcgcttcgcc gacctggact gcgaacggcg ccgcgaaaac gttgcttgcc
     3661 acgaaaaagc cagccaggac ctggatgcgc atgcggcgcc attctagccg cttgcgccgg
     3721 atcacttatg attggccttt ccggagagcg cagcgcttgg cgaagatact gagcaagctt
     3781 ccgcggggcg agaaaatcgg cgtcgcgtat tccggcggcc tggatacctg cgccgccgtg
     3841 cgctggatgc gcgcgaacgg cgcgattccg tacacgtaca gcgcgaatct cggccagccc
     3901 gacgaggccg acttcgagga catcccgcgc aaggcgcttg cctacggcgc ggagaaggcg
     3961 cgccttgtcg aatgccgccc gcagctcgtc gccgagggaa tcgccgccat ccagtgcggc
     4021 gcgttccaca tctcgacggg cggtacgacg tacttcaaca ccacgccgct cggccgtgcg
     4081 gtgaccggga cgatgctcgt ggtcgccatg cgcgaggacg gggtggacgt ctggagcgac
     4141 ggcagcacct acaagggcaa cgacatcgag cgcttttatc gctatgggct gctcacgaac
     4201 cccgcgctgc gcatctacaa gccctggctc gaccagcgct tcatcgacga gctcggcggc
     4261 cgcaaggaga tgtccgagta tctttcccgg gcgggcgtgg cgtaccggac gagcgcggag
     4321 aaggcgtatt cgaccgactc caacatcctc ggcgccacgc atgaggcgaa ggacctcgag
     4381 ttcctgaaca aggccatggc gatcgtcacg ccgatcatgg gcgtggcgtt ctggcgcgaa
     4441 gacgtgccgg tgaagtccga gaaagtcgcc gtgcgcttcg agcagggcca gccggtcgcg
     4501 ctcaacggcc agcaattccg caactccgtc gagcttctgc aggaggcgaa tgcgatcggc
     4561 gggcgccatg gcctgggcat gtcggaccag atcgagaacc gcatcatcga ggccaagagc
     4621 cgcggcatct acgaggcgcc gggcatggcg ctgctccaca tcgcctacga gcgcctggtc
     4681 accgggatcc acaacgagga caccatcgag cagtaccgcg cgaacggccg gcggctgggc
     4741 cggctgctgt accagggccg gtggttcgac ccccaggcca tgatgctgcg cgagaccgcg
     4801 cagcgctggg tggccgccgc ggtaagcggc gaggtgacgc tcgagctgcg ccgcggcaac
     4861 gactattcga tcctcgacac caccagcccc aacctcacct acaagcccga gcgcctcacc
     4921 atggagaagg gcgaggaggt gtttttcgcc cccgccgacc gcatcggcca gctcacgatg
     4981 cgcaacctcg acatcgccga cacgcgcgac aagctgcgca tctacggcga aaccggcctc
     5041 ctcggcgagc cgaaggaagg cgccgtgccg cttctcgggg gcggcgagcc gaagaaaaaa
     5101 gcctagggac tatttctttc ccaggaccag gaattcgagc agggccttct gcgtgtgcag
     5161 ccggttttcc gcctcgtccc acaccaccga ctgcggcccg tcgatcaccg cggcggccac
     5221 ttcctcgccg cggtgcgccg gcaggcagtg catgaagagc gcgtccttct tcgccgcgcg
     5281 catcatgtcg gcgtcgacct gccagtcctg gaagtcgcgc cgccgctcct cgttctcgtc
     5341 ctcgaagccc atcgaagtcc acacgtcggt ggtgacgagg tcggcgccgc gggcggcggc
     5401 catggggtcg gcgaaggcct cgctgttgcg attcatcctt tccttctcca cctcgtagcc
     5461 cggcggggtc gagacgttaa gccggaagtc gaagagctcc gccgcctgca gccaggtgtt
     5521 gcacacattg ttcgagtcgc cgatccaggc gacggtacgg ccgcggatcg ggccgcgatg
     5581 ctcgacgtac gtgaagacat cggcgaggat ctggcaggga tggtactcgt tggtaagccc
     5641 gttgatcacc ggcacgcggg acgcgccggc gaagcgctcg atgatcgcct gctcgaaagt
     5701 gcgcaccatg atgacgtcgc acatgcgtga gatcacctgg gccgcgtcct ccaccggctc
     5761 gccccggccg agctgcgagt cgcgcgtgta gagatagatc gcggcgccgc ccagctggtg
     5821 catgccggcc tcgaacgaca tgcgcgtgcg cgtcgaaggc ttctcgaaga tcatcgccag
     5881 cgtgcggtcc tcgagcggcc agtagcgctc gtagcgcttg aacttgtcct tgatccagcg
     5941 cgtgcgcgcg aacacatgct cgagctcgtc gagcgaaaga tccctgagtt gcaggatgtg
     6001 gcgtaccgcc atggcgcgcc ggctcagcgc gccgcggccg tggcctgaga gctgcgctcg
//
LOCUS       DASUNT010000561         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1037567, whole genome shotgun sequence.
ACCESSION   DASUNT010000561 DASUNT010000000
VERSION     DASUNT010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037567"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFX94_11375"
     CDS             complement(<1..435)
                     /locus_tag="VFX94_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768826.1"
                     /translation="MKESSLCRKGRDAYARRAWQQAYQELLLADRAAPLGGEDLERLA
                     VASYLTGRESEFLSCYERAHRAHLAREAPEHAARCAFWLGLAALLRGEPGQASAWLAR
                     ARRLVGRKACPVQGYLLLPDAERQLQAGDFQAACKTASRAAVI"
     gene            complement(410..1582)
                     /locus_tag="VFX94_11380"
     CDS             complement(410..1582)
                     /locus_tag="VFX94_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX5768827.1"
                     /translation="MLPLQGIRVVEFAHMVMGPSCGLVLADLGAEVIKVEPLAGDNTR
                     RLEAAGAGFFPVFNRNKKSLAVDLQKPEGRELILKLLRKADVLTENFRPRALDKLGFS
                     YEKLSKENPKLVYCSLKGFLKGPYEHRLALDEVTQMMGGLAYMTGLPDRPLRAGSSVI
                     DIMGGTFAAVGILAALREREKTGRGMRVTSALFESTAYLVAQHMAQFELTGEAPPPMS
                     VKRPAWGIYDIFDTAGGGRLFVGVVTDTQWEVFCREFGAPDLAGDPRLRSNGLRVKER
                     GWLVPRINDIMMKHTQAELTAKLEAIGLPFAPIAKPWDLLEDPHLKASKGLVETRINT
                     KTIRVPALPLALDGERLGKRADPPGIGQHGAELLSQLGYSSADLARLKDEGVVALP"
     gene            complement(1583..2401)
                     /locus_tag="VFX94_11385"
     CDS             complement(1583..2401)
                     /locus_tag="VFX94_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010110572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5768828.1"
                     /translation="MASLRGKTLFVTGGSRGIGLAIALRAAADGANIAIAAKTVEPHP
                     KLPGTIHTAARDIEAAGATCLPLQCDIRDEAQIARAVAATVERFGGIDVLVNNASAIG
                     LTGTMETPPKRFDLMFGINVRGTYMTSQACIPHLRKAANPHILTLSPPLNMNPRWFRH
                     HVAYTMAKYGMSMCVLGMAEEFREAGIAVNALWPKTIIATAALQMIPGIDPARGRKPE
                     VMADAAHWILTQPARSCSGNFFIDEDVLRKAGVADLRRYAVDPSKSLAQDLFLD"
BASE COUNT          387 a          828 c          838 g          416 t
ORIGIN      
        1 gataaccgct gcacgcgaag cggtcttgca ggcggcctgg aaatcacctg cctgtagctg
       61 ccgctcggcg tctggcagca gcaaataccc ctgcacggga caagccttgc gcccgacgag
      121 gcgccgggcc cgggcgagcc atgcgctcgc ctggcccggc tcgccgcgca gcagcgcggc
      181 gagaccgagc cagaacgcac agcgcgcggc gtgctcgggc gcttcgcgcg cgagatgcgc
      241 gcggtgggcg cgctcgtaac agctgagaaa ctcgctctcg cggccggtca ggtaggacgc
      301 cacggcaagc cgttccagat cctcgccgcc cagcggcgcg gcgcggtcgg cgagcaggag
      361 ctcctgatag gcctgctgcc acgcgcgccg cgcataggcg tcgcggccct tacggcaaag
      421 cgacgactcc ttcatccttc aagcgcgcga gatcggcgct ggagtacccc agttgcgaga
      481 gcagttccgc gccgtgctgg ccgattcccg gagggtcggc gcgcttgccc aggcgctcgc
      541 cgtcgagcgc aagcggcagg gcgggaactc ggatcgtttt cgtgttgatc cgggtttcga
      601 caaggccctt cgacgccttg aggtgcgggt cctccagcag gtcccaaggc ttggcgatgg
      661 gcgcgaacgg caggccgatc gcctcgagct tcgcggtgag ctcggcttgc gtgtgcttca
      721 tcatgatgtc gttgatccgc ggcacgagcc agcctcgctc cttgacccgc aagccgttgc
      781 tcctcagcct gggatcgccc gcgaggtcgg gcgccccgaa ctcccggcag aaaacctccc
      841 attgcgtatc agtcaccacg ccgacgaaga ggcgtccgcc gccggcggta tcgaagatgt
      901 cgtagatgcc ccaggcgggg cgcttcaccg acatgggcgg aggcgcctcg ccggtgagct
      961 cgaactgcgc catgtgctgc gcgacgaggt aggccgtgct ctcgaaaagc gcgctcgtga
     1021 cccgcatccc gcgcccggtc ttttcgcgct cgcgcagcgc cgccaggatc ccgaccgcgg
     1081 cgaacgttcc gcccatgatg tcgatcaccg aggagccggc gcgcagcggc cggtcgggaa
     1141 gccccgtcat gtaagcaagg ccgcccatca tctgggtgac ctcgtcgagg gcgagacgat
     1201 gctcgtacgg gcccttgaga aagcctttca gcgagcagta cacgagcttc gggttttctt
     1261 tggaaagctt ctcgtacgaa aagccgagct tgtcgagcgc cctgggacga aagttctccg
     1321 tcagcacgtc ggccttcctg agaagcttga gaatcaactc tcgtccttcg ggcttctgca
     1381 agtcgaccgc gaggcttttc ttgttgcgat tgaacaccgg gaagaagccc gcgcccgcgg
     1441 cttcgagccg ccgcgtgtta tcgccagcga ggggctccac cttgatcacc tcggcgccga
     1501 ggtcagcaag cacgagcccg cacgaggggc ccatgaccat gtgggcgaat tcgacgaccc
     1561 ggatgccttg gagcggcagc attcagtcga ggaagaggtc ctgggcgagg gacttcgaag
     1621 gatcgaccgc gtacctgcgc agatcggcga cgcccgcctt gcgcaggacg tcctcgtcga
     1681 tgaagaaatt gccgctgcag gaccgggcgg gctgggtgag gatccagtgc gccgcgtcgg
     1741 ccatcacttc gggcttgcgc ccgcgggccg ggtcgatgcc ggggatcatc tgcagcgcgg
     1801 ccgtggcgat gatggttttc ggccacagcg cgttcaccgc gatcccggcc tcgcggaact
     1861 cctccgccat gccgagcacg cacatgctca tcccgtactt cgccatggtg taggcgacat
     1921 ggtgcctgaa ccagcgcggg ttcatgttga gcggcggcga gagcgtcagg atgtgcgggt
     1981 tcgccgcctt gcgcagatgc gggatgcacg cctgcgacgt catgtacgtt ccgcgcacgt
     2041 tgatgccgaa catgaggtcg aagcgcttcg gcggtgtctc catcgtgccg gtaaggccga
     2101 tcgcgctcgc gttgttgacc aggacgtcga tgccgccaaa tcgctcgacg gtggccgcaa
     2161 ccgcgcgcgc gatctgcgcc tcgtcgcgaa tgtcgcattg cagcggcagg cacgttgcgc
     2221 ctgccgcctc gatgtcgcgc gccgcggtat ggatggttcc cggaagcttc ggatgcggct
     2281 ccacggtctt cgcggcgatc gcgatgttcg cgccgtcggc ggcggcgcgc agcgcgattg
     2341 caaggccgat gcccctgctg cccccggtca cgaagagcgt cttgcctctc aacgacgcca
     2401 ttgcggcttg cgcttctcga tgaaggcgcg cacgccctcg gcgaaatcgg gctccgaggc
     2461 gctctgcgc
//
LOCUS       DASUNT010000562         3859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_599382, whole genome shotgun sequence.
ACCESSION   DASUNT010000562 DASUNT010000000
VERSION     DASUNT010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599382"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..281)
                     /gene="atpE"
                     /locus_tag="VFX94_11390"
     CDS             complement(6..281)
                     /gene="atpE"
                     /locus_tag="VFX94_11390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX5768829.1"
                     /translation="MDKLQYLAMIQAYTSIGIGLMIGVGAAGACIGVGVMCSRFLEAA
                     ARQPELTNSLQAKVFLLLGLIDASFIIGVGLAMMFAFANPLLAVIPK"
     gene            complement(328..1152)
                     /gene="atpB"
                     /locus_tag="VFX94_11395"
     CDS             complement(328..1152)
                     /gene="atpB"
                     /locus_tag="VFX94_11395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX5768830.1"
                     /translation="MAAGNTLTPTEYIQHHLTFFTKPLGEGGFWTLHVDSLVTALLVG
                     VVAIGFIWWVVRGATSGVPNKRQAFVELAFDFIDDQVKGVFHHGDRNRFVAPAALTVF
                     VWVLLMNAMDFLPVDIMAKILGALGLHEWRLVPTADVNTTFALSLSVWLLMIFFAIAV
                     KGLGGWIHELTCTPFGVKPWAAAPFLVVFNVLFNLIEYVSKPLSHSLRLFGNMYAGEI
                     IFLLLWLLAATGLAGTIFAVFLGLGWAIFHILIVVLQAFIFMMLTVVYIALAHEHH"
     gene            complement(1162..1530)
                     /locus_tag="VFX94_11400"
     CDS             complement(1162..1530)
                     /locus_tag="VFX94_11400"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEX5768831.1"
                     /translation="MLRRLSKPIRTVLAWQAAATLAMTLAGAAFIGIHGAVSAAAGGL
                     VSILAGLAAALIAARVNGKSAGGILAGALRAEAVKVGLSVLLLWLVLANYPEAIVVIV
                     IGSFLVTMLLFSMAFFVREY"
     gene            complement(1587..2426)
                     /locus_tag="VFX94_11405"
     CDS             complement(1587..2426)
                     /locus_tag="VFX94_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX5768832.1"
                     /translation="MKAKGLGRGLDALLGGAAAEEAPREALLTLPVARIRPGRYQPRT
                     KMDQQALAELAASIRSQGLMQPLLVRPVERERYELIAGERRWRAAQMAGLEEVPALVR
                     EVPDEAALAMSLIENIQRENLNPMEEAAGIQRLIEEFRMTHEQAADAVGRSRSATTNL
                     LRLLKLARPVQEMLMEGALEMGHARALLALDGARQIEAGKRVAAKGLSVRETEALVQG
                     LLRGPAVPRKRNDRDLARLEEEIAGRLGTTVEIRAGSKGTGKIVLHYSSLDHLDELIK
                     KLR"
     gene            complement(2423..3196)
                     /locus_tag="VFX94_11410"
     CDS             complement(2423..3196)
                     /locus_tag="VFX94_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX5768833.1"
                     /translation="MPRVLAVVNQKGGVGKTTTTVNLAAALSQAGRRVLLVDMDPQGN
                     ATMGSGIDKRTLARTVYHALLGLGELAGIRVRAERGGYDLVPANRDLAGAEVELVELP
                     ARETRLKSALERVAADYEYILIDCPPSLSLLTVNALAAADQVLIPMQCEYYALEGLSD
                     LVGTIKRVRANLNPALDIAGLLRTMYDPRNTLSQQVSSELESHFGDKVFRTLVPRNVR
                     LAEAPSYGVPGVLWDPGSKGAQAYIALASELLQRQEAYS"
     gene            complement(3175..3756)
                     /gene="rsmG"
                     /locus_tag="VFX94_11415"
     CDS             complement(3175..3756)
                     /gene="rsmG"
                     /locus_tag="VFX94_11415"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX5768834.1"
                     /translation="MTLVLPREAHERLLDYVALLAKWNRTYNLTAIREPLAMVSHHLL
                     DSLSVLPHLPLGAKQARLADAGSGAGLPGIPLAIARPGWRVALAESSQKKAAFLRQAA
                     IELELDNVEVHEGRVQVWRPQPLFDLVISRAFAALGDFISACRHLVTPGGMLAAMKGA
                     PPKDLSDNCSSIALRVPLLQAQRHLILCPASSP"
BASE COUNT          711 a         1263 c         1250 g          635 t
ORIGIN      
        1 ccgcgctact tcgggatcac ggcaagcagc ggattggcga acgcgaacat catcgccagg
       61 cccacgccga tgatgaacga cgcgtcgatc aggccgagca gcaggaacac tttcgcctgc
      121 agcgagttgg tgagctcggg ctggcgcgca gcggcttcga ggaagcggct gcacatgacg
      181 ccgacgccga tgcaggcgcc tgccgcgccg accccgatca tcaggccgat cccgatgctg
      241 gtgtaggcct ggatcattgc cagatactgc agtttgtcca tttttgattt ccctttctgc
      301 gcagagtgcg gtaacggtta gtgccggcta gtgatgctcg tgggcaaggg cgatgtacac
      361 gacggtgagc atcatgaaaa tgaacgcctg cagcaccacg atcaggatgt ggaaaatcgc
      421 ccagcccagg ccgaggaaga cggcgaagat cgtgccggcg agcccggtcg ccgccagcag
      481 ccacagcagc aggaagatga tctcgcccgc gtacatgttg ccgaaaagcc gcagcgagtg
      541 cgacagcggc ttggaaacgt attcgatgag gttgaaaagc acattgaaca ccacgagaaa
      601 cggcgccgcg gcccagggtt tcacgccgaa gggcgtgcag gtgagctcgt ggatccagcc
      661 gccgaggccc ttcaccgcga tggcgaagaa gatcatgagc agccagaccg agagcgagag
      721 cgcaaaggtg gtgttcacgt ccgccgtggg caccagccgc cattcgtgca ggccgagcgc
      781 gcccaggatc ttggccatga tgtcgaccgg cagaaaatcc atcgcattca tcagcagcac
      841 ccagacgaag accgtcagcg ccgcgggcgc gacgaagcgg ttgcggtcgc cgtgatgaaa
      901 aacccccttg acctggtcgt cgatgaagtc gaacgccagc tcgacgaacg cctggcgctt
      961 gttcggcacg ccggaggtcg caccgcgcac cacccaccag atgaagccga tggcgacgac
     1021 gccgacgagc agcgcggtga ccagcgaatc gacatgcaat gtccagaagc cgccttcacc
     1081 aagcggcttg gtgaaaaacg tcaggtggtg ctggatgtac tccgtcgggg taagcgtatt
     1141 gcctgcggcc atctggatgc gtcaatactc gcgaacgaaa aacgccatcg aaaaaagcag
     1201 catggtgacc aggaacgacc cgatgactat aaccacgatc gcttcgggat agttcgccag
     1261 caccagccac agcagaagaa cgctcaggcc gactttcacg gcttcggcgc gcagtgcacc
     1321 ggccaagatt ccacctgccg atttcccgtt gaccctggca gctatcagcg cggctgccag
     1381 gccggcaagg atgctgacca ggccgccggc ggccgcggaa accgcgccgt ggataccgat
     1441 gaacgccgct cccgcgagcg tcatcgcaag cgtggctgct gcctgccacg cgagcacggt
     1501 gcggataggc ttgctcagcc ggcgaagcac agcgttcggt ttcttcgcgg cgagcaaagc
     1561 cgcgaaagta tatcggaact gggcgctcac cggagcttct tgatcagctc gtcgaggtga
     1621 tcgaggctcg agtagtgcag cactatcttg cctgttcctt tgcttccagc gcgaatctcg
     1681 accgtcgtgc cgagccgccc ggcgatttcc tcctccaggc gcgcgagatc gcggtcgttt
     1741 cgcttgcggg gcaccgccgg cccgcgcaac agcccttgca ccagcgcctc ggtttcgcgc
     1801 accgacagcc ccttcgccgc cacgcgcttg ccggcctcta tctggcgcgc cccgtcgagc
     1861 gcgagcagcg cgcgcgcgtg ccccatctcg agcgcgcctt ccatcagcat ctcctgcacc
     1921 ggccgcgcga gcttcagcag ccgcagaagg ttcgtcgtgg cgctgcgcga acgcccgacg
     1981 gcgtccgcgg cctgctcatg tgtcatcctg aactcttcga tcaggcgctg gattcccgcg
     2041 gcttcttcca tcggattgag gttctcgcgc tggatgttct cgatgagcga catggcgagc
     2101 gccgcttcgt ccggcacttc acgcacgagg gccgggactt cctcgagccc ggccatctgc
     2161 gcggcgcgcc agcgccgctc gccggcgatc agctcgtagc gctcacgctc caccggccgc
     2221 acgaggagcg gttgcatgag tccttgggag cggatcgacg ccgcgagctc ggcaagggct
     2281 tgctggtcca tcttggtccg cggctggtat cgcccgggcc ggatgcgcgc cacgggcagc
     2341 gtgagcagcg cctcgcgggg cgcctcctcg gcggcggcgc cgccgagcag cgcgtcgagg
     2401 ccccgcccga ggcctttggc cttcatgagt acgcctcctg tcgttgcaac agctcactgg
     2461 caagcgcgat gtacgcctgc gctcccttcg agcccggatc ccacagaacg cccggcacgc
     2521 cgtagctagg cgcctcggca agacggacat tgcgcggcac gagcgtgcga aagaccttgt
     2581 ccccgaagtg agattccagc tcgctcgaca cctgctgcga aagcgtgttg cgcgggtcgt
     2641 acatcgtgcg cagtagaccc gcgatgtcga gcgccggatt gagattcgcc cgtacgcgct
     2701 tgatcgtgcc caccaggtcc gacaggcctt cgagcgcgta gtactcgcat tgcatcggga
     2761 tcagcacctg atcggcggcg gcgagcgcat tgaccgtgag aaggctgagc gagggcggac
     2821 aatcgatcag gatgtattca taatcggccg cgacgcgctc gagcgccgac ttaaggcgtg
     2881 tctcgcgtgc cggcagctcg accagctcta cctccgcgcc ggcgagatcg cggtttgccg
     2941 gcacgaggtc gtagcccccg cgctcggcgc gcacgcggat cccggcaagc tctcccaggc
     3001 cgagaagcgc atggtagacg gtgcgcgcga gcgtgcgctt gtcgattccg ctgcccatgg
     3061 tggcgttgcc ttgaggatcc atatcaacca gcagcacccg ccgaccggct tgcgacagtg
     3121 cggcggcaag gttaacggtc gtcgtcgtct tcccgacgcc gcctttctgg ttcactacgg
     3181 cgaggacgcg gggcatagga taaggtgacg ctgtgcctgg agaagcggta cacgaagggc
     3241 gattgagctg cagttgtcgc tcagatcttt tggtggcgcg cctttcatgg cggcgagcat
     3301 gccccccggt gtgacgagat gccggcaggc cgaaatgaaa tcgccgaggg cggcaaatgc
     3361 gcgcgagatc accaggtcga agagcggctg cgggcgccac acctgcaccc ggccctcgtg
     3421 cacctcgaca ttgtccagct caagttcgat cgctgcctgg cgcaagaacg cggctttctt
     3481 ctgactcgat tctgccagcg cgacgcgcca gccggggcgc gctatcgcga gtggaatccc
     3541 cggcagcccg gcgcccgagc cggcgtcggc gagacgcgct tgcttagccc cgagcggcag
     3601 gtgcggcaac accgaaagcg agtcgagaag atgatgggaa accatggcga gcggctcgcg
     3661 tatcgcggtc aggttgtagg tgcgattcca cttcgccagc agcgccacgt agtcgagcaa
     3721 ccgctcgtgc gcttcccggg ggagcacgag cgtcatttcc ccgaggccgc ggttaagcgc
     3781 ggcgcgcggc gtcatgccct tttcttgtcg agccctcgaa agccccgttt caaatgcaca
     3841 agcagaagag agattgccg
//
LOCUS       DASUNT010000563         4997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1125905, whole genome shotgun sequence.
ACCESSION   DASUNT010000563 DASUNT010000000
VERSION     DASUNT010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125905"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VFX94_11420"
     CDS             <1..486
                     /locus_tag="VFX94_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5768835.1"
                     /translation="PGTFLDVASVQADPLTFAAEPWLDLLASTADPARRRLHLQVRRW
                     TLDETPMAAALLDEVANRLYRDNQFAEGRLEVGGRLATPGAITAPVLGVLDPNSRIVP
                     PSSIEAYRGRTRSSGVTLATYTGESGVVMQHVGVLVGRNAHRTLWPQIATWLQSVTHS
                     M"
     gene            491..691
                     /locus_tag="VFX94_11425"
     CDS             491..691
                     /locus_tag="VFX94_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768836.1"
                     /translation="MTRSQIRQFVETSSVERIREVLDLLQKLKRHVPLSELPQHEVAF
                     LLDLRDHEDFFKTLADAKLQQS"
     gene            complement(769..1350)
                     /locus_tag="VFX94_11430"
     CDS             complement(769..1350)
                     /locus_tag="VFX94_11430"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5768837.1"
                     /translation="MNRALILFTSLLIAPLASAQLAVPANEPYVGQPGKDVIWVPTPP
                     SLVEKMLDMASVTPRDFVVDLGSGDGRNVIAAAKRGARALGVEYDPNLVQVSRRNAAA
                     EGVADRASFVQGDMFEADISKATVLALFLLPDNLERLKPKFERLRPGTRIVTNGYQIA
                     GWDAKEVGVAGGDCAPWCTAYLYVVPTKPNRQD"
     gene            1451..2578
                     /locus_tag="VFX94_11435"
     CDS             1451..2578
                     /locus_tag="VFX94_11435"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX5768838.1"
                     /translation="MLKAVIAAAVSVLFTSFAVQAQAPKSVKVQSTWPASITLQDHLR
                     ILAERIDKLTQGSVKIEALAAGQIVPPFEVLDATNKKVLDGFHAIAYYWVGKNPAAAL
                     FAGPPGGPFGMDHMDFLGWLYVGGGLDMWRDFYQNDLKLNVIVWPAHPSSPQALGWFK
                     KPLKGVADFKGMKCRQTGLNAEVYAKLGQSVVNMAGGEIVPAAQRGVIDCAEWVGGVE
                     DLRLGLPGVFKYHYTPGMHENNSIGEFGLNLDVWKGLSPQQQEAVNSAVKDTFITWIT
                     RWQKQNADAIEEMITKHGVQIRRTPPDILLASLKAWDEVAAEHSSKHPTFKKVYASQK
                     EYAAKVVPAKRYMFPPYSFAANYYWPQEGGGTAAKKDAKKP"
     gene            2562..2879
                     /locus_tag="VFX94_11440"
     CDS             2562..2879
                     /locus_tag="VFX94_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768839.1"
                     /translation="MPKSPEPEETLRWPTNLDRAGIERRVIEVREVARKNALPEIVGF
                     FEGIEHMSPAQIGANVVAALTWLQDKPQHRALTMQLEMVAMNLKNLKDGNSGTDGNSG
                     TEA"
     gene            complement(2932..3141)
                     /locus_tag="VFX94_11445"
     CDS             complement(2932..3141)
                     /locus_tag="VFX94_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768840.1"
                     /translation="MALALAPVAARQEEEKPNKIERAAKKTGQAIENTADRAGKWAGR
                     TVNRAGKFIENTAQKTDKAIRRALE"
     gene            3445..4563
                     /locus_tag="VFX94_11450"
     CDS             3445..4563
                     /locus_tag="VFX94_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5768841.1"
                     /translation="MPGMQVTRQMWSVFKQAVQAWSDDYAPSMGAALSYYTLFSIAPL
                     FLIVIAVAGWFFGEEAARGELTAGLQRLMGEEGARAIEEMVANASKPAEGIIATVVGL
                     LVLMLGATTVFGELQNALDRIWRAPAREKLSGLWRLLRARLLSIGMILGIAFLLMVSL
                     VLDAVLQWLGKLWGTGAWEAVAQVANTLVGFALTTAVFALIYKLIPRAKVEWHDVWVG
                     AAVTALLFTIGKFLIGLYIGRSAVASSFGAAGSLAVVMIWVYYSAQIFLLGAEFTWVY
                     SHAHGSRRGQKRPGAVEVKTPETAPAAAAPSMPPPVRVAPAPLPRLPAARELPVHRRK
                     PLVSFGVAAAVGAIAGVLVRTRPKLFFQRSRPRLFSRW"
     gene            4596..>4997
                     /locus_tag="VFX94_11455"
     CDS             4596..>4997
                     /locus_tag="VFX94_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768842.1"
                     /translation="MGGAAKAVVAALALAIAGGTGYWLYVEKQKTEQQERVLALLGDT
                     TRQLRQALNAPPAPDVVSSIDSNLKLAKAPRHPELALAAEHYIHGAREIVRRRSDAER
                     LAREAAMSRRALAMHMSAASARDTYWIRVASD"
BASE COUNT          815 a         1672 c         1665 g          845 t
ORIGIN      
        1 cccggcacat tcctcgacgt cgcgagcgtg caggccgacc cgctcacctt tgccgccgag
       61 ccgtggctcg acttgctcgc gagcacggca gacccggcgc gtcgccgcct gcacttgcag
      121 gtgcgccgct ggactctcga cgagactccc atggcggcgg cgctgctgga cgaagtggcg
      181 aaccggctat atcgggacaa ccagttcgcc gaagggcggc tcgaagtcgg cggccggctc
      241 gccacgcccg gcgccatcac cgcgcccgtg cttggcgtgc tcgatccgaa ttcccgcatc
      301 gttccgccct cgtcgatcga ggcctaccgc ggtcgcacgc ggagctccgg cgtaacgctc
      361 gcgacctaca ccggggaaag cggcgtggtg atgcagcacg tcggggtgct ggtcgggcgt
      421 aacgcgcacc gaacgctttg gccgcagatc gcaacctggc tgcaatccgt gacacactcc
      481 atgtagatag atgacgcgca gccagatccg gcagttcgtc gagacttcca gcgtcgagcg
      541 catccgcgag gtgctcgacc tgttgcagaa gctcaagcgc cacgtgccgc tgagcgagtt
      601 gccgcagcac gaggtggcgt ttctcctcga cctgcgcgat cacgaggact tcttcaagac
      661 gctcgccgac gcgaagctcc agcagtcctg aaggcctggc gggagcagga cccggcgctg
      721 acgccggatc tcgagttcac ctgcgagttc gacccgcgga gcgcgccgct agtcctgccg
      781 gttcggtttc gtcggcacta cgtaaaggta cgcggtgcac cagggggcgc agtcgccgcc
      841 ggcgacgccc acctccttcg cgtcccagcc ggcgatctgg tagccgttgg tgacgatgcg
      901 cgttcccggc cgcagccgct cgaacttggg cttgaggcgc tccaggttgt cgggcaggag
      961 gaacagcgct agcaccgtcg ccttcgaaat atcggcctcg aacatgtcgc cctggacgaa
     1021 gctcgcccgg tcggcgaccc cttccgcggc cgcattgcgg cgcgagacct gcaccaggtt
     1081 ggggtcgtac tcgacgccga gcgcccgggc gccgcgcttc gccgcggcga tgacgttgcg
     1141 tccgtcgccc gagccgaggt cgacgacgaa gtcgcggggc gtcacggacg ccatgtcgag
     1201 catcttctcg acgagcgagg ggggcgtcgg cacccagatc acgtccttgc cgggctgacc
     1261 gacgtagggc tcattggccg ggacggcgag ctgggcgctc gcgagtggtg caatcaggag
     1321 gctggtgaag agaatcagtg cgcggttcat gcccaaggtt aacgcactag caggctttct
     1381 tgttccgcgc ccctagtctt gccggagcgc cgttgaaggc cctagcatgc ggtttcaggg
     1441 agggtatcgc atgctcaagg ctgtcatcgc agcggccgtt tcggttcttt tcacgtcgtt
     1501 cgcggttcag gcgcaggcgc cgaagtcggt gaaggtgcag tcgacctggc cggcaagcat
     1561 cacgctccag gatcatttgc ggattcttgc cgagcgcatc gacaagctta cccaaggctc
     1621 ggtcaagatc gaggcgctcg ccgccggcca gatcgtgccg ccgttcgagg tgctcgacgc
     1681 caccaacaag aaagtgctcg acggcttcca cgcgatcgcc tactactggg tcgggaagaa
     1741 tcctgcggcc gcgctcttcg ccgggccgcc gggaggcccc ttcggcatgg accacatgga
     1801 tttcctcggc tggctgtacg tcggcggcgg gctggacatg tggcgcgact tctaccagaa
     1861 cgacctcaag ctcaatgtga tcgtgtggcc ggcgcacccg tcgagcccgc aggccctcgg
     1921 ctggttcaag aagccgctga agggcgtcgc cgacttcaag ggcatgaagt gccgccagac
     1981 cgggcttaac gccgaggtct acgccaagct cggccagagc gtggtgaaca tggctggcgg
     2041 cgagatcgtg cccgcggcac agcgtggcgt gatcgactgc gccgagtggg tcggcggcgt
     2101 ggaagacctg cggctcgggc tgccgggcgt cttcaagtac cactacaccc ccggcatgca
     2161 cgagaacaac tcgatcggcg agttcgggct gaacctcgac gtgtggaaag gcctctcgcc
     2221 gcagcagcag gaggcggtca actccgcggt caaggacacc ttcatcacct ggatcacgcg
     2281 ctggcagaag cagaacgccg acgcgatcga ggagatgatc acgaagcacg gcgtgcagat
     2341 ccgtcgtacc ccgcccgaca tcctcctcgc ttcgctcaag gcgtgggacg aggtggcggc
     2401 cgagcactcc tcgaagcacc cgacgttcaa gaaggtgtac gcgtcacaga aggagtacgc
     2461 ggcgaaggtt gtgccggcga agcgctacat gttcccgccg tactctttcg cggccaacta
     2521 ctactggccg caggaaggcg ggggcaccgc agcgaagaag gatgccaaaa agccctgaac
     2581 cggaggaaac ccttcgctgg ccgaccaacc tcgatcgcgc cggcatcgag cgccgcgtga
     2641 tcgaggtgcg tgaggtggcc cgcaagaacg cgcttcccga gatcgtcggc ttcttcgaag
     2701 gcatcgagca catgagcccc gcgcagatcg gcgccaacgt cgtcgccgcg ctcacctggc
     2761 tgcaggacaa gcctcagcac cgcgcgctca ccatgcagct cgagatggtg gcgatgaacc
     2821 tgaagaacct gaaggatggg aattcgggga cggacgggaa ttcggggact gaggcctaac
     2881 caattcgggg accgagccct ttaggctccg tcgccgaatt gcgcccgcga attactccag
     2941 cgcccgcctg atcgccttat cggtcttctg agcggtgttc tcgatgaact tgcccgcccg
     3001 gttgacggtc ctgcccgccc actttccggc ccgatcagcg gtgttttcta tcgcctgacc
     3061 ggtcttcttc gcggcgcgct cgatcttgtt gggcttttcc tcttcctggc gcgccgctac
     3121 aggcgccagg gcgagcgcca aggcgagcac tgcagtcgcc atacgacgcc agattgcggc
     3181 cggagcaact tcgcgaaaaa acatggagta acacctccca gctacattct aggggaggaa
     3241 agaggtacaa cccttggcag acgacgatct cgaccagatc cgcgcgtact acgtcacgct
     3301 gcgcgaggtg atcatcctga attcgggcgg tggaacgacg ccgaatcgcg cgcgcgggtc
     3361 cagcagctct gccgctccgg gagatcccga cgcgcgccgc gcacgcgccc gccgatcgcc
     3421 tgcgcggcgg cacgctgatt gctgatgccg ggcatgcagg tgacgaggca gatgtggtcg
     3481 gtcttcaagc aggccgtcca ggcctggtcc gacgattacg cgcccagcat gggcgcggcg
     3541 ctctcctact acactctctt ttcgatcgcg ccgcttttcc tcatcgtgat cgcggtcgcg
     3601 ggctggttct tcggcgagga agcggcgcgc ggcgagctga ccgccgggct gcaacgactg
     3661 atgggcgagg agggcgcgcg tgcgatcgag gagatggttg cgaatgcctc gaagcctgcg
     3721 gagggcatca tcgccaccgt cgtggggctc ctggtgctca tgctcggcgc aaccacggtg
     3781 ttcggggagc tgcagaacgc gctcgaccgg atatggcgcg cgccggcgcg cgagaagctg
     3841 agcgggctgt ggcgcctgct gcgcgcgcgg ctcctctcga tcggcatgat cctcggcatc
     3901 gcgttccttc tcatggtgtc gcttgtcctc gacgccgtcc tccagtggct cggcaagctg
     3961 tggggcacgg gagcatggga ggcggtggcg caagtggcga acaccctcgt cggcttcgcg
     4021 cttacgactg cggttttcgc gctgatctac aagctcatcc cgcgcgccaa ggtcgaatgg
     4081 cacgacgtct gggtcggcgc cgcggtcacc gcgctgctct tcaccatcgg caagttcctc
     4141 atcgggctct acatcgggcg cagcgccgtc gcttcgtcgt tcggcgccgc cggctcgctc
     4201 gccgtggtga tgatctgggt ctactactcg gcgcagatct ttcttctcgg cgcggagttc
     4261 acctgggtct attcccacgc gcatggctcg cgccgcggcc agaagcgccc cggcgcagtg
     4321 gaagtgaaga cgccagaaac ggcgccggcc gctgcagcgc cctccatgcc gcccccggtg
     4381 cgtgtcgcgc ccgccccgct gcccaggctt cccgcggcgc gggagctgcc tgtgcaccgc
     4441 agaaagccgc tcgtttcgtt tggcgtcgca gccgccgtgg gtgcgatcgc cggcgttctg
     4501 gtgcgcaccc ggccgaaact gttcttccag cgctcgcgtc cgcgactttt ttcacgctgg
     4561 tagatcgaat cgcctttcag ctatcctccc cggccatggg aggtgctgcg aaggcggtcg
     4621 tagccgcgct cgcgctcgcc atcgcgggcg gcacgggcta ctggctttac gtcgagaagc
     4681 agaagacgga gcagcaggag cgggtgctcg cgctcctcgg cgacaccacc aggcagctgc
     4741 gccaggcgct gaacgccccg cctgcgcccg atgtggtgtc gagtatcgac agcaacctga
     4801 agctcgccaa ggcgccgcgc catccggagc tcgcgctagc cgcggagcac tacattcacg
     4861 gcgcgcgcga gatcgtgcgc cggcgaagcg acgccgagcg ccttgcgcgc gaggcggcga
     4921 tgagccgacg ggcgctcgca atgcacatgt ccgcggcgag cgcgcgcgat acctactgga
     4981 tccgcgtcgc ctccgat
//
LOCUS       DASUNT010000564         3330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_775382, whole genome shotgun sequence.
ACCESSION   DASUNT010000564 DASUNT010000000
VERSION     DASUNT010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775382"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2032)
                     /gene="glgX"
                     /locus_tag="VFX94_11460"
     CDS             complement(<1..2032)
                     /gene="glgX"
                     /locus_tag="VFX94_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX5768843.1"
                     /translation="MEAPARALVAPSAVWRGRPYPLGASWDGAGVNFALFSKHAEAVE
                     LCLFDPKGRREIERVAVRERTDFVWHCYLPEARPGLLYGYRVYGPHDPDRGHRFDYNK
                     ILLDPYARLIRGKVSAGIGRCEVVDLAFTWGDDRPPRTPWQDTVIYELHVKGFTQLHP
                     EVPEQLRGTYAGLASQAAISYLKDLGVTAIELLPVHTIADERRLLHHGLRNYWGYNSI
                     GFFAPDMRYSASGTLGEFKSMVKTLHAAGIEVILDVVYNHTGEGDHTGPTLSFRGIDN
                     AIYYRLDPAHLRRYLNYAGTGNSLNLAHRVVLALAMDSLRYWVQEMHVDGFRFDLAAT
                     LARNAEGAFDRNVAFLAAVRQDPVLSQVKLVAEPWDLAEGGYQLGNFPPGWAEWNDKY
                     RDAVRSYWKGDEGLIGELASRISGSQDIFQRSGRAPVASINFVTTHDGFTLHDLVSYE
                     RKRNEANLEGNGDGSDNHRSWNCGVEGPTDQVSIRNLRDKQKRNFLATLLLSQGVPLL
                     VAGDEMGRTQLGNNNAYCHDSELSWIDWQLDADARALAEFAKKLLLLRKSHPLLRRRT
                     YPKPENTVWLSPEGREMSEQDWKLPFARCIGCLMVGERLAERDERGTPIMDDDLLVLM
                     NAHHDAVEFHLPDEAWALLVDTAQPDTNSLGKVYRLEARSIALLARPRRTSPA"
     gene            complement(2041..2448)
                     /locus_tag="VFX94_11465"
     CDS             complement(2041..2448)
                     /locus_tag="VFX94_11465"
                     /inference="COORDINATES: protein motif:HMM:NF027482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
                     /protein_id="HEX5768844.1"
                     /translation="MLALLGPAVGSAAQSDVTGADVDEIRTVIHRQIDAFRRDDAHSA
                     FALVSPKVQQTFGTPERFLDTVRMAYRAVYRPARVSFLELVVMGSDVVQPVQVTDRSG
                     AVWVAYYAMQRQKDGSWRTHGCRLVQPTRIISA"
     gene            complement(2463..3077)
                     /locus_tag="VFX94_11470"
     CDS             complement(2463..3077)
                     /locus_tag="VFX94_11470"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5768845.1"
                     /translation="MPVRISNKVLTAVIVAGGALLLSAAPPYAVAPDLAGIACTLATA
                     EPQSYADREPLDVTQQALVQHLSKRFFIAPAATERVVAAAHRSAAEVGLDPLLVLAVI
                     SIESRFNPIAESVMGAKGLMQIIPKYHLDKLRAAGGEHAVFDPESNIHVGTRILQEYV
                     YRTGTLEAGLQFYNGALRDGSAQYAHKVLAERMRLEQVLRPRDG"
BASE COUNT          563 a         1052 c         1134 g          581 t
ORIGIN      
        1 gagctgggct tgtgcgcctg ggtctagcga ggagtgcgat cgagcgcgct tcgagccggt
       61 aaacctttcc gagggagttc gtgtcgggct gcgccgtgtc cacgagcagc gcccaggcct
      121 catcgggaag gtggaattcg acagcatcgt ggtgcgcgtt catcagcacg agcaagtcgt
      181 cgtccatgat cggcgtgccg cgctcgtcgc gctcggcgag gcgttcgcca accatgaggc
      241 atccaatgca gcgcgcgaag ggcagcttcc agtcctgctc gctcatttcc cggccctcgg
      301 gagagagcca gacagtgttc tcgggcttcg ggtaagtgcg ccggcgaaga agcggatggc
      361 tttttcgcag caagagaagt tttttcgcga actctgccag cgcccgggcg tccgcgtcga
      421 gctgccagtc gatccaggag agctcgctgt cgtgacagta agcattattg ttgccgagct
      481 gggtgcgtcc catttcgtcg ccggccacaa ggagcggcac accttgcgag agcaacagcg
      541 tcgcgaggaa gttgcgtttc tgcttatctc tcaagttcct tatcgaaacc tgatcggtgg
      601 ggccctccac gccgcagttc cagctgcggt ggttgtccga gccgtcgccg ttgccttcca
      661 gattggcttc gttacgcttg cgctcgtagc tcacgaggtc gtgcagcgtg aagccgtcgt
      721 gcgtggtcac gaagttgatg ctagcgacgg gcgcgcggcc gctgcgctgg aaaatatcct
      781 gggagcccga gatgcgcgag gcgagctcgc cgattaagcc ctcgtcgcct ttccagtacg
      841 agcgcactgc atcccgatac ttgtcgttcc actctgccca gccgggcggg aagttgccga
      901 gctggtagcc gccctcggcg aggtcccagg gctcggcgac gagcttcacc tgcgacagca
      961 ccgggtcctg gcgcaccgcg gcgaggaagg cgacgttgcg gtcgaacgcg ccctcggcgt
     1021 tgcgcgcgag cgtggcggcg aggtcgaagc ggaagccatc gacatgcatt tcctggaccc
     1081 agtagcgcag gctgtccatg gcgagcgcga gcaccacgcg atgcgccagg ttcaggctgt
     1141 taccggtgcc cgcgtagttg agatagcgac gcaggtgcgc aggatccagg cggtagtaga
     1201 ttgcgttgtc gatgccgcgg aagctgagcg tgggtccggt atggtcgccc tcgccagtgt
     1261 ggttgtagac gacgtcgaga atgacctcga tgccggccgc gtgcagcgtc ttcaccatgc
     1321 tcttgaattc gcccagcgtg ccgctcgctg aatagcgcat gtccggggcg aagaagccga
     1381 tcgagttgta gccccagtag ttgcgcaaac cgtggtgcag aagccgccgc tcgtcggcga
     1441 tggtgtggac cggcaacagc tcgatcgccg taacgcccag atcctttaga taggaaatgg
     1501 ctgcttggga tgcgaggccg gcatacgtgc cgcgcaattg ttccggcacc tcgggatgga
     1561 gttgcgtaaa gcccttgacg tggagctcgt agatgaccgt gtcctgccac ggcgtgcgcg
     1621 gcggccggtc gtcgccccag gtgaacgcca ggtccacgac ctcgcaccta ccgatcccgg
     1681 ccgaaacttt tccccggatc aggcgggcat agggatcgag caggatcttg ttgtaatcaa
     1741 agcgatggcc gcggtcgggg tcgtgcgggc cgtacacgcg gtagccgtag agcaggccgg
     1801 gccgcgcctc gggcaggtag cagtgccaga cgaaatcggt gcgctcgcgc accgcgacgc
     1861 gctcgatctc gcgccggccc ttcgggtcga aaaggcatag ctccacagct tcggcgtgct
     1921 tcgagaacag cgcgaagttg actccggccc cgtcccagct cgctcccagg ggataggggc
     1981 ggccgcgcca gaccgcgctc ggcgcgacca gggctcgtgc gggcgcctcc acgcccccga
     2041 ttatgcagaa attatccggg tcggctgaac gaggcggcag ccgtgggtgc gccagctgcc
     2101 atccttctgc cgctgcatag catagtacgc cacccaaact gcgccggagc ggtcggtgac
     2161 ctgcaccggc tgcacgacgt cgctgcccat caccacgagc tcgaggaaag ataccctggc
     2221 cggtcggtac accgcgcggt aggccatgcg cacagtgtca aggaagcgct cgggagtgcc
     2281 gaaggtttgc tgtaccttag gcgacacgag ggcaaacgcg ctgtgggcgt cgtcccgccg
     2341 aaaggcgtcg atctggcgat gaatgactgt ccggatttcg tcgacgtcgg cgccggtgac
     2401 atcgctttgc gcggcggagc caaccgccgg accgaggagc gccagcaaaa gaagaagagt
     2461 cctcatccgt ctcgcgggcg caacacctgc tcaagacgca tgcgctcggc gagcaccttg
     2521 tgggcgtatt gcgccgaccc gtcgcgcaag gcgccgttgt agaactgcag gcctgcctcc
     2581 agcgtgccgg tgcggtagac gtactcctgg aggatgcgcg tgccgacgtg gatattgctc
     2641 tccgggtcga acaccgcgtg ctcgccgccc gccgcgcgca gcttgtcgag gtggtacttc
     2701 gggatgatct gcatgagccc cttggcgccc atcacgctct ccgcgatcgg gttgaagcgc
     2761 gattcgatcg agatcaccgc gagtactagc agcggatcga gcccgacttc ggctgccgac
     2821 cggtgcgccg cggctaccac gcgctcggtc gccgccggag cgatgaaaaa gcgcttcgac
     2881 agatgctgaa cgagcgcctg ctgcgtcacg tcgagcggct cgcgatctgc gtaggactgc
     2941 ggctcagccg tcgccagcgt gcaggcgatc cccgccaggt cgggtgcgac ggcgtacggg
     3001 ggcgcggctg aaaggagcag cgctccgccg gcaacgatta ccgcggtgag gaccttgttg
     3061 gaaatccgga cgggcattcg cgcaccccgg gggttggacc gaatggtagg gcgcagcctg
     3121 aacccgggct atcgcggcga cttgaatttc ctgtcgcggc gcgactacac gcggtctccc
     3181 gcacgctaca tccgtacgcg cctcggggcg atagtggcgg gaccccggca ctttggccgc
     3241 gctcgcgtcc agccgctcaa gtcgcccgcg ccctggcaag ccgcgagtag agatcggcat
     3301 agcgacgagc ggctgccgac cagctgaagt
//
LOCUS       DASUNT010000565         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_775407, whole genome shotgun sequence.
ACCESSION   DASUNT010000565 DASUNT010000000
VERSION     DASUNT010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775407"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VFX94_11475"
     CDS             complement(<1..389)
                     /locus_tag="VFX94_11475"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768846.1"
                     /translation="MDFELVRQAARQKYTRPFLILDTAIVRNKIRRFSAAMPRVRPHY
                     AVKANPDRRVLKVLLQEGAGFEIASTAELDLLLALGVPAAEIFYSNPMKSRDAIAYAA
                     AKGVEWYVVDSVEELRRVHEIKPDARQ"
     gene            complement(673..1239)
                     /gene="dcd"
                     /locus_tag="VFX94_11480"
     CDS             complement(673..1239)
                     /gene="dcd"
                     /locus_tag="VFX94_11480"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX5768847.1"
                     /translation="MTLKSDQWIRRMALERRMIEPFEARQVKELAGKKVVSYGTSSYG
                     YDIRCSTEFKIFTNINSTIVDPKNFDEKNFVDYRGEVCIIPPNSFALARTVEYFRIPR
                     NVLTICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPARVYANEGVAQVIFL
                     ESDEVCETSYRDRGGKYQGQKGVTLPRI"
     gene            1269..2120
                     /locus_tag="VFX94_11485"
     CDS             1269..2120
                     /locus_tag="VFX94_11485"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX5768848.1"
                     /translation="MSCPFAVRPLTPWLGAEISGIDLAHELSEALFAALYRAFLRYQV
                     LLFAPQDLSPARQVAFGRRFGELQVHVMNQYHADGHPELYRLSNLDEQGRPSGKHPDK
                     GTLAWHTDGSWQRVTGQATIIYGELMPETGGETHFCDMYGAYERLDARWKARIAGLRA
                     VHNLDFSRSRRHGEDPLTEAQRRAKPPVDHPVVRTHPETGRKCLYLGDHAEYILGMPY
                     DEGRALIEELNALAVHADLTYEHRWRPLELLVWDNRCVMHRATAYDAATQPRVIRRCT
                     VLGEVPA"
     gene            2117..>2502
                     /locus_tag="VFX94_11490"
     CDS             2117..>2502
                     /locus_tag="VFX94_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006395973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HEX5768849.1"
                     /translation="MKHGIALCFVVMASTAVAGGLAPFSEREIRAILAHGPWPAPATS
                     DASNAASGKRTAIELGQRLFFDTRLSGDGRFSCGTCHVPERNWTDNRTRGFAAAEVER
                     NTPTLMNLRLSRAFGWAGSAGTLWEQ"
BASE COUNT          427 a          812 c          802 g          461 t
ORIGIN      
        1 tactgcctgg cgtcgggctt gatctcgtgc acgcgccgca gctcttccac gctgtcgacg
       61 acgtaccact ccactccctt ggccgccgcg tacgcgatgg cgtcgcgcga cttcatgggg
      121 ttggagtaga aaatctccgc cgccggcacg ccaagcgcga gcagcaggtc gagctccgcc
      181 gtcgaggcga tctcgaagcc cgcgccttcc tgtaaaagca ccttgagcac gcgccggtcc
      241 ggattggcct tcaccgcgta atgcgggcgc acgcgcggca tggccgcgct gaaccgacgg
      301 atcttgttcc gcacgatggc ggtgtccagg atcaggaagg gccgggtgta cttctggcgt
      361 gctgcctggc gcacgagctc gaagtccaag ctgacttcgg cgtgcgtgtc aaatagggct
      421 tgcggctctt gcttcggtac tgcggcggat tgtctggtgc gtgtgatgct gctactgcgg
      481 tcccccatca aagtctctcc tcgtaacggt cgaatcacta agtttcggga atatcccgga
      541 cgcaaacgcc gtcggtaaac acgatcaact cggtgaacat cacaaatctc cttctgcgct
      601 gaagcaaaaa gaaaaaaact tgaccggagt ataggggcat gaccccgcaa gtcaagggct
      661 gcgggtgaat ttttagatgc gcggcagtgt gacgcccttc tggccctggt acttgccgcc
      721 gcggtcgcgg taagacgtct cgcagacttc gtcggactcg agaaagatca cctgcgccac
      781 gccttcgttg gcatagacgc gcgcggggag cggcgtcgtg ttggagaact ctagcgtcac
      841 gtgtccttcc cactcgggtt cgagcggcgt gacgttgacg atgatgccgc agcgcgcgta
      901 ggtcgacttc ccgaggcaga tggtgagcac gttgcgcgga atgcggaaat attccacggt
      961 gcgcgcgagc gcgaaggagt tcggcgggat gatgcagacc tcgccgcggt agtcgacgaa
     1021 gttcttctcg tcgaagttct tggggtcgac gatggtcgag ttgatgttgg tgaagatctt
     1081 gaattcggtt gagcagcgga tgtcgtagcc gtagctcgag gtgccgtacg acacgacctt
     1141 cttcccggcg agttccttga cctggcgcgc ctcgaagggc tcgatcatcc gccgctcgag
     1201 cgccattcgc cggatccact ggtctgattt gagggtcatt tcttctccac tggactaaac
     1261 ttgattctat gtcctgtcca ttcgccgtgc ggccgctgac gccgtggctc ggcgccgaga
     1321 tttcgggcat cgacctggcg cacgagcttt ccgaggcgct attcgccgcg ctgtaccggg
     1381 cgtttctgcg ctaccaggtc ctgctcttcg cgccgcagga cctttccccg gcacgccagg
     1441 tggctttcgg ccgccgcttt ggcgagctcc aggtccacgt gatgaaccag tatcacgccg
     1501 acgggcatcc cgagctctac cgcctgtcga acctcgacga gcaaggcaga ccgagcggga
     1561 agcacccgga caaggggacg ctcgcctggc acaccgacgg ctcctggcag cgcgtgacgg
     1621 gccaagcgac catcatttac ggcgagctca tgcccgagac gggtggggag acccacttct
     1681 gcgacatgta cggcgcctac gagcggctcg acgcgcgctg gaaggcgcgt atcgcaggcc
     1741 tgcgggccgt gcacaacctc gatttctcgc gcagccggcg ccacggcgag gatcccctga
     1801 ccgaagcgca gcggcgtgcg aagccgccgg tcgaccaccc ggtagtgcgc acgcatcctg
     1861 aaacgggacg caaatgcctc taccttgggg atcacgccga atacatcctt ggcatgccct
     1921 acgacgaggg gcgcgctctg atcgaggagc tcaacgcgct cgccgtccat gcggatctca
     1981 cctacgagca ccgctggagg ccgctcgagc ttctcgtctg ggacaaccgc tgcgtgatgc
     2041 atcgcgcgac ggcctacgac gcggcgaccc aacctcgcgt catccggcgc tgcaccgtgc
     2101 tcggcgaggt gccggcatga agcacggcat agcgctttgc ttcgtcgtca tggcgtccac
     2161 ggcggtggcc ggcgggctcg caccgttcag cgaacgcgaa atccgcgcca tcctggccca
     2221 tgggccatgg ccggcgccgg cgacgagcga tgcgagcaac gctgcctcgg gcaagcgcac
     2281 tgccatagag cttgggcagc gcctgttctt cgatacgcgc ctctccggcg acgggcgctt
     2341 ctcctgcggc acctgccatg tgccggagcg caactggacg gacaaccgca cccgcggctt
     2401 cgctgccgcg gaggtcgagc gcaacacgcc cacgctcatg aacctgcgcc tgtcgcgcgc
     2461 cttcggctgg gccggcagcg ccggcacgct ttgggaacag ag
//
LOCUS       DASUNT010000566         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1037931, whole genome shotgun sequence.
ACCESSION   DASUNT010000566 DASUNT010000000
VERSION     DASUNT010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037931"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="VFX94_11495"
     CDS             complement(<1..1036)
                     /locus_tag="VFX94_11495"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEX5768850.1"
                     /translation="MAGSARGGRHYGGADPRGRRSGGGVGPESRPAAGTGGGIAAYAG
                     QAALVAAAYFGTAKLSLLAAIPPGYASAIWPPSGIALAAVLLGGDRLWPAIWAGSFAA
                     NVTVDGALFSSALIATGSTLQALVLAVLVRRHIGVPRRFERIEDVIKFVLLAALGSII
                     APTFALIPLTLGYGMPPDELAGNWSTWWQGDACGVLVFAPLVLAWMTPGAQAWPARRL
                     LEAGLFAVVVLGAAHLVFRSGIAPGFLIVPLVVWAAFRFGQREVMTAIAAVCALALWV
                     MPPESLPLLPLFAATLVFTGLVLTVALSGLERAMLELESRVRSRTAELEEAKLAAERA
                     NQAKSQFLANM"
     gene            complement(919..2097)
                     /locus_tag="VFX94_11500"
     CDS             complement(919..2097)
                     /locus_tag="VFX94_11500"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEX5768851.1"
                     /translation="MSRDYELQTLGVRAGQARTEFQEHSEALFLTSSFVFKNARQAAA
                     RFAGEEEGMVYTRYTNPTVAMLQERLAALEGAESSVATASGMSAILTTAMTHLKSGDH
                     VVCSNAVFGATIQLFNNVLSRFGVETTYASPTKLEDWRQAIRPNTRMFFLETPSNPLT
                     EVTDLAGLSRIAKDAGVLLAVDNVLCTPALQRPLELGADLVVHSATKYLDGQGRVIAG
                     AVAGSRALVGDPMTAFLRTAGPTLSPFNAWIVLKGLETLQVRMLAQSAAALELAQWLE
                     TRKEVSRVFYPGLPSHPQYELARRQQRAGGALLSFEVKGGRAAAWRVIDSTRLVSLTA
                     NLGDVKTTIVHPASTTHGRISPEARAAAGITEGLIRVAVGLEAVSDLKADLQRGLEAE
                     "
     gene            complement(2255..>2627)
                     /locus_tag="VFX94_11505"
     CDS             complement(2255..>2627)
                     /locus_tag="VFX94_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX5768852.1"
                     /translation="QMARESGARKVFFASAAPPVRYPNVYGIDMPTRAELIAAHRSEE
                     EVAREIGADALVYQDLDALKDAVQRCNPRLTSFETSCFDGHYITGDITTDYLRSIEIR
                     RDAKRDSNEEEAPQLDLNLAA"
BASE COUNT          454 a          896 c          887 g          390 t
ORIGIN      
        1 acatgttggc gaggaactgg gacttcgcct ggttcgccct ttcggcggcg agcttggctt
       61 cctcgagctc ggcggtacgg ctgcgcacgc gggactcgag ctcgagcatg gcgcgctcga
      121 gcccgctcag cgccacggtc agcacgaggc cggtgaagac gagcgttgcg gcaaagagcg
      181 gcagcagggg gagcgactcc ggcggcatga cccaaagggc aagcgcgcat accgccgcga
      241 tcgccgtcat gacctcccgc tggccgaagc ggaaggcggc ccaaacgacg agcggcacga
      301 tgaggaaccc gggtgcgatg cccgacctga acaccaggtg cgcggcgccg agcaccacca
      361 cggcgaagag cccagcctcg agcagcctgc gcgccggcca cgcctgtgcg ccgggcgtca
      421 tccacgcgag cacgagcggc gcgaacacca gcacaccgca tgcatcgccc tgccaccacg
      481 tcgaccagtt gcccgccagt tcgtcgggcg gcatgccata gccgagggtg agcggaatca
      541 gcgcgaacgt cggcgcgatg atgctgccga gcgccgcgag cagcacgaac ttgatgacgt
      601 cctcgatgcg ctcgaagcgc cgcggcaccc cgatgtgccg gcgcaccagg acggcgagca
      661 caagcgcctg gagcgtgctg ccggtggcga tcaacgcgga agagaacaat gcgccgtcca
      721 ccgtgacatt ggcggcgaag ctgcccgccc agatcgcggg ccacagccgg tctcccccga
      781 gcagcacggc agcgagcgca atgcccgaag gcggccagat cgcgctcgcg tagcccggcg
      841 gaatcgcggc gagcagcgag agcttggcgg tgccgaagta cgcggcagcg acaagcgcag
      901 cctgcccggc gtaagctgct attccgcctc cagtccccgc tgcaggtctg ctttcaggtc
      961 cgacaccgcc tccagaccga cggccacgcg gatcagcccc tccgtaatgc cggccgccgc
     1021 gcgcgcttcc ggcgatatcc tgccatgcgt cgtgctcgcc ggatgcacta tcgtagtctt
     1081 cacgtcgccc aggttggcgg tgagggagac gaggcgcgtc gagtcgatga cccgccaagc
     1141 cgcggcgcgg ccgcccttga cctcgaagga cagcagcgca ccgccggcgc gctgctgccg
     1201 ccgggcgagt tcgtactgcg ggtgcgacgg cagcccggga tagaacaccc ggctgacttc
     1261 tttgcgcgtc tcgagccact gcgcgagctc cagcgccgcg gccgattgag cgagcatgcg
     1321 cacttgcagc gtctcgagcc ctttcagcac gatccaggcg ttgaacggcg acagcgtggg
     1381 ccccgcggtg cgcagaaacg cggtcatcgg gtcgcccacc agcgccctgg atccggccac
     1441 ggccccggcg atcacccgtc cctgcccgtc gagatacttc gtcgcggaat ggaccaccag
     1501 gtccgcgccc agctcgagcg gccgctgcag cgcaggagtg caaagcacgt tgtcgacggc
     1561 gagaagcacg ccggcgtcct tcgcgatacg cgaaaggccg gcgaggtcgg tcacttcggt
     1621 aagggggttg gacggtgttt cgaggaagaa catccgggtg ttgggccgga tcgcctgccg
     1681 ccagtcctcg agcttcgtcg gcgacgcgta cgtcgtctcg acgccgaagc gcgaaagaac
     1741 gttgttgaag agctgaatcg tggcgccgaa gactgcgttg gagcagacca cgtggtcccc
     1801 gctcttcaga tgcgtcatcg cggtagtcag gatcgccgac atcccggagg cggtcgcgac
     1861 cgagctttcc gcgccttcca gcgcggcgag ccgctcctgc agcatcgcca ccgtcggatt
     1921 ggtgtagcgc gtgtacacca ttccctcctc ctcgccggcg aagcgggcgg cggcctgcct
     1981 cgcgttcttg aagacaaagc tcgaggtgag gaacagcgcc tcggaatgct cctggaactc
     2041 ggtgcgggcc tggccggcgc gcacgcccag ggtttgcagc tcgtagtctc tggacataaa
     2101 aaaacccggg cctctagtgc agcggaccgg gtctcgtggg agaactgcgc ggcacgcttt
     2161 agccgaattt attacgcgcc cgcaagctgt ggcccttcaa atcggcgcga cggcaagact
     2221 atatcaggcg gccagccaaa tgtcagccgc gaggttacgc ggctagattc aggtccagtt
     2281 ggggggcttc ttcctcgttc gagtcccgct tcgcatcacg ccggatctcg atcgagcgca
     2341 ggtagtccgt ggtgatatcg cccgtaatgt aatgaccgtc gaaacaggag gtctcgaagc
     2401 tcgtcagccg ggggttgcag cgctgcaccg cgtccttgag cgcgtcgagg tcctggtaaa
     2461 cgagcgcatc ggcgccgatc tcgcgagcca cctcctcctc gctgcggtgc gccgcgatga
     2521 gctcggcgcg cgtcggcatg tcgatcccgt acacgttcgg gtagcgaacc ggcggagccg
     2581 cggaggcgaa gaacaccttg cgcgctcccg actcgcgcgc catctgc
//
LOCUS       DASUNT010000567         2415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1302712, whole genome shotgun sequence.
ACCESSION   DASUNT010000567 DASUNT010000000
VERSION     DASUNT010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302712"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1813)
                     /gene="dsbD"
                     /locus_tag="VFX94_11510"
     CDS             complement(35..1813)
                     /gene="dsbD"
                     /locus_tag="VFX94_11510"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012763150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEX5768853.1"
                     /translation="MKRFLVGLLVAVAAAQAQAQLSRLLKSDELLEPEKAFRFSARAV
                     EGAVEVDFAIADGYYMYRDKFRFAAEGNPAVRLGAPEFPSGRRHRDEFFGETETYRNQ
                     VRIRIPAEGEGRFELKVTSQGCADVGVCYVPMESRASLQAVSGTFVSKGPRTVDEAPR
                     FSIFASDIEIARLFEGNLVLVLAGFFVLGMLLTFTPCVLPMIPILSAIIAGEGKRLDK
                     WRGFALSLTYVLGMALAYALAGVAAAYSGSLLAAALQNAWILGAFAAVFVVLALSMFG
                     FYELRLPGFLHRHLHSAHNRLRGGQIASVAAMGVLSAVIVSPCVAAPLAGALLYISQT
                     RDVALGAAALFAMALGMGVPLVAVGVSEGALLPRAGAWMEGVRKFFGVLLLAVAIWIV
                     SPVIAPMAQMLAWGALFIGSAMFLRAIDPLPEAASGWSRLWKGVGIVALVTGIAFVVG
                     AFSGAQDVLRPLGAISRNSPVAAPLPWTRVASLAELEARLRAPGKTVMLDFYADWCVS
                     CKEMEAFTFSDRKVRAQLDQMLLLQVDVTANNEHDKALLKRFSLFGPPGIIFFDAEGR
                     EIKGLRVIGYQNAERFLKTLSLLTAP"
     gene            complement(1810..2136)
                     /gene="cutA"
                     /locus_tag="VFX94_11515"
     CDS             complement(1810..2136)
                     /gene="cutA"
                     /locus_tag="VFX94_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX5768854.1"
                     /translation="MESTKLLVLTNLPDRATAEKLADMLIGEQLAACVNILAPCRSVY
                     RWKGEVQHDEEHPMLIKTTQERYAALEQALRAGHPYELPEIIAVPIERGLPAYLDWLA
                     AETTRA"
     gene            2152..>2415
                     /locus_tag="VFX94_11520"
     CDS             2152..>2415
                     /locus_tag="VFX94_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5768855.1"
                     /translation="MHKVTVQPSGQGFEVQEGESVLAAALRHGVMLPYGCKNGACGSC
                     KGKIIAGSVDYGHYPARVLSEAERGHGKALFCQAKPLEDLVIEC"
BASE COUNT          450 a          796 c          818 g          351 t
ORIGIN      
        1 gacggcatcc tgacttactt cgccatggac gccgctaagg cgctgtcaaa agcgaaaggg
       61 tcttgaggaa acgctcggcg ttctggtagc cgatcacgcg caggcccttg atctcgcgcc
      121 cctcggcatc gaagaagatg atgccgggcg ggccgaacag cgagaagcgc ttgagcagcg
      181 ccttgtcgtg ctcgttgttc gccgtgacgt ccacctgcaa gagcagcatc tggtcgagct
      241 gcgcgcgtac cttgcggtcg gagaaggtga aggcctccat ttccttgcac gagacgcacc
      301 agtcggcata gaagtccagc atcaccgtct tgcccggagc ccggagcctc gcttcgagct
      361 cggcgagcga ggcgacccgg gtccacggca gcggcgccgc aacgggggag ttgcgcgaga
      421 tcgcgcccag cggccgcagc acgtcctgcg ctcccgagaa tgcaccgacg acgaaggcga
      481 tgccggtcac caacgcgacg atgccgacgc ccttccacag ccggctccag cctgaggctg
      541 cctcgggcag cgggtcgatc gcgcgcagga acatcgccga gccgatgaag agcgcccccc
      601 aggcgagcat ctgcgccatg ggcgcaatca ccggcgagac gatccagatc gccaccgcga
      661 gaagcagcac gccgaaaaac ttgcgcaccc cttccatcca cgcgcccgcc cggggaagca
      721 gtgccccctc ggacacgccc acggcgacga gcggcactcc catgccgagc gccatggcga
      781 aaagcgcggc agcgccgagc gccacgtcgc gcgtctgcga gatgtagagc agcgcccctg
      841 cgagcggcgc ggcgacgcac gggctgacga tcaccgcgga gagcacgccc atcgcagcta
      901 ccgaggcgat ctggccgccg cgcaggcgat tgtgggcgga atgcagatgg cgatgcagga
      961 agcccggcag ccgcagctcg tagaaaccga acatggagag cgcgagcacc acgaacacgg
     1021 ccgcaaaagc gcccaggatc cacgcgtttt gcagcgcggc ggcgagcagc gagccggaat
     1081 acgcggccgc gacgccggca agcgcatagg cgagcgccat tccgagcacg taggtgagcg
     1141 aaagggcgaa cccccgccac ttgtcgagac gcttgccttc gccggcaatg atcgccgaga
     1201 ggatcgggat catcggcagt acgcacggcg tgaacgtgag cagcatgccg aggacgaaga
     1261 agccggcaag cacgaggacg agattgcctt cgaaaaggcg cgcaatctcg atgtcgctcg
     1321 cgaatatcga gaagcgcggc gcttcatcca ccgtgcgcgg gcctttggag acgaaagtcc
     1381 cggaaacggc ctgcaagctt gcccgcgact ccatcggcac gtagcagacg ccgacgtcgg
     1441 cgcagccttg cgatgtgacc ttgagctcga agcgcccctc gccctcggcc gggatgcgga
     1501 tgcgcacctg gttgcggtag gtctccgtct cgccgaagaa ttcgtcgcgg tggcgcctcc
     1561 cgctcgggaa ttccggcgct ccgaggcgaa ccgccggatt gccctcggcc gcgaagcgga
     1621 acttgtcgcg gtacatgtag tagccatcgg cgatcgcgaa gtcgacctcg accgcgcctt
     1681 cgacggcccg tgcgctgaag cggaacgcct tttcgggctc gaggagctcg tcgctcttca
     1741 gtaatcggga aagctgcgct tgcgcctgtg cggcagccac agccacgagt agcccgacca
     1801 ggaaacgctt cacgctcttg tcgtctcggc agccagccaa tcgaggtagg cgggcaggcc
     1861 gcgctcgatt gggaccgcga tgatctcggg aagttcgtag gggtgcccgg cgcgcagcgc
     1921 ctgctcgagc gcggcgtagc gctcctgcgt ggtcttgatg agcatcgggt gctcttcgtc
     1981 gtgctgcacc tcgcccttcc agcgatacac cgagcggcac ggcgcgagga tgttgacgca
     2041 cgcggcgagc tgctcgccga tcagcatgtc ggcgagcttt tccgccgtcg cgcggtcggg
     2101 caggttggtg aggaccaaga gcttggtaga ctccatgcgc ctattctagc catgcacaaa
     2161 gttacggttc agccgagcgg ccagggcttc gaggtccagg aaggcgagtc ggtgctcgca
     2221 gccgcgctgc ggcatggcgt gatgctgccc tatggctgca agaacggcgc ctgcggctcg
     2281 tgcaagggaa aaatcatcgc cggctcggtc gactacggcc actaccccgc gcgcgtgttg
     2341 agcgaggccg agcgcgggca cggcaaggcg cttttctgcc aggcgaagcc gctggaggac
     2401 ctggtgatcg agtgc
//
LOCUS       DASUNT010000568         6224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_250409, whole genome shotgun sequence.
ACCESSION   DASUNT010000568 DASUNT010000000
VERSION     DASUNT010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250409"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VFX94_11525"
     CDS             complement(<1..508)
                     /locus_tag="VFX94_11525"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX5768856.1"
                     /translation="MLVAGYAGFGALAAAHDIPLTLAVASTVVLWALPGQIVLIEMHT
                     LAAPLAATVLAVMLTSARFLPMTLVLIPELRDARHRPWKYYIAAQLLSLSGWTMAMAR
                     FPGIPRPQRLAWFYGFALVLMTVSAASTAAGYLGADHLPPLARLALVFMAPMYYLLIL
                     TGAARERSA"
     gene            complement(557..1462)
                     /locus_tag="VFX94_11530"
     CDS             complement(557..1462)
                     /locus_tag="VFX94_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5768857.1"
                     /translation="MPGSIPASASVEELSRHDARVGWLGIALDFTRRRPLGAIGAAII
                     LAMVVLAATAGWLAPHHPLETDYGAMLAPPDAQHWLGTDAFGRDVLSRLIYGSRTALM
                     VGLGASLLGATLGSLIGVASAYFGGRVDLIVQRIMDVFFAFPVIILALAVVAVLGTGA
                     SNVILAIAVPMVPRCARVVRASALAVREMPYVDAARASGYGDGRIILRHMLPNVMAPI
                     LIMATAFLGEAILLEASLSFLGLGVQEPVAAWGLMLRGAAVQFAESAPWMAIFPGIAI
                     SLAVFGFNLFGDSLRDALDPRLRAQ"
     gene            complement(1428..2384)
                     /locus_tag="VFX94_11535"
     CDS             complement(1428..2384)
                     /locus_tag="VFX94_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5768858.1"
                     /translation="MGAYVLKRLAAMIPTLLGVAVLIFILLRIVPGDVVEARYLAQGS
                     QFLSQDLMNQERAKLGLDKPLWQQFTSWMAGLARLDFGVSMWTGAPITEEIKLRFALS
                     LQLALMASTIATLLAIPLGILAAVKQDTWVDYAVRIFSIAGLATPSFWLGILMILVML
                     TVFKWIPPMVYTPFWVNPWENLAQLIWPALAVGYRYSAVGTRMMRSAMLEVLREDYIR
                     TARAKGLWPNMILARHALKNAILPVLTIISLEFAFLLGGLVVTEQVFNLNGLGMLFVE
                     AIARRDYTLTQALVMLVATVFLLVNFFVDIAYAWLDPRIRFS"
     gene            complement(2432..4024)
                     /locus_tag="VFX94_11540"
     CDS             complement(2432..4024)
                     /locus_tag="VFX94_11540"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5768859.1"
                     /translation="MKLVYLLAVAAGVALFGPAPLQAQRNGGELVFPVPSEPPSYDGH
                     REETFGLIHPIAPFYNTLLRVDPNDKSGTKPHPSVAESWTVSPDGKTYTFKIRRGVKF
                     HDGSELTARDVKATYDKIIFPPPGVGSSRKGQYADVEKVDAPDATTVVFRLKEPSGSF
                     ISSLLSPYNFLYKADILAKDPHWYEKNIMGTGPFTFAEHVRGSHVIGKKNPDYWDKGK
                     PYLNGFRAVFMRDSAAQVAAVRGERAHIQFRGFSPAERDSIVSTLGKKVVVQESPWDC
                     ILLVAINHEQKPFDDKRVRRALTLALDRHQASKSLSKIAIVKEVAGVQVPGTPFATPP
                     EELNKLAGYWPDIEKSRAEARRLLKEAGVPEGFKFTFTNRGIPMPYEPLGVWLIDQWR
                     RVGLNVDQQVLEPAAYYTPLRKGDFQVAMDFQCGYIVEPDLDMYKFLSVDRNPANYAR
                     YKDPVLDALYDKQSRATNPEERKQLVRQFEKRLLDDEVHYLMTLQWHRIIPHSAKVKG
                     WTITPSHYLNNTLDTVWLEGGD"
     gene            complement(4092..5087)
                     /locus_tag="VFX94_11545"
     CDS             complement(4092..5087)
                     /locus_tag="VFX94_11545"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX5768860.1"
                     /translation="MSTTLLEVDNLVKHFPVGGGAFAGPGGTVRAVDGVSFTLKRGET
                     LGLVGESGCGKTTTGRCILQLERPTSGRVLFDGVDLAAIDAVALRAVRRRVQVIFQDP
                     YSSLNPRMTVGQILAEPLKVHGIIRSRSAREARVRELLEQVGLLPQHARRYPHQLSGG
                     QRQRVGIARALAMEPELIVCDEPVSALDVSIQAQIINLLEDLQSRLGLTYLFIAHDLS
                     VVRHISDRVAVMYLGRIVELADRAALYEEPLHPYTRALLSAVPIPDPDVEATRERTVL
                     RGEVPSPLKPPSGCVFHPRCPIAVERCTAEIPPLREIRPGHWAACHLAQVGLSQT"
     gene            complement(5084..6085)
                     /locus_tag="VFX94_11550"
     CDS             complement(5084..6085)
                     /locus_tag="VFX94_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5768861.1"
                     /translation="MASLLEVRNLRTTFLTSAGVVRAVDGVSWDIREGETVALVGESG
                     CGKSVSALSVMRLVAEPAGRIEAGEILYKGRDLLKLSEPEMQRVRGREIAMVFQEPMT
                     SLNPVLSIGRQLTEGLEIHIRMSPAQARERAVELLATVGIPDPQRRLAQYPHHFSGGM
                     RQRMMIAMALACNPSLILADEPTTALDVTIQAQILELMRDLARRLGVAMLIITHNLGV
                     VARYADRVNVMYAGRIIEQGAARELYANPRHPYTLGLLRSVPRLDEPRRARLDPIQGQ
                     PPDLTRLPPGCAFAPRCAYRVARCAEVPPLEAVGQAAGAPHTSACWEAMRLAKELAA"
BASE COUNT         1088 a         2014 c         2187 g          935 t
ORIGIN      
        1 tggcgctgcg ctcacgcgcc gcgccagtga ggatcagcag gtagtacatc ggcgccatga
       61 agacgagcgc aagccgcgcg agcggcggca ggtgatcggc gccaaggtag ccggcagccg
      121 tcgaggcggc gctcaccgtc atcagcacga gcgcgaagcc gtagaaccag gcaagtcgtt
      181 gcgggcgcgg gatgccggga aagcgcgcca tggccatggt ccagcccgag agcgagagca
      241 gctgagcggc gatgtagtac ttccacggcc gatgccgggc atcgcgcaac tcgggtatca
      301 gcaccagcgt catcggcagg aagcgcgccg aagtcagcat cacggcaagc accgtcgcgg
      361 cgagaggcgc cgcgagggtg tgcatctcga tcagcacgat ctgcccgggc agcgcccaga
      421 gcaccaccgt cgaggctacg gcgagcgtga gcgggatgtc gtgcgcggcg gcaagggcgc
      481 cgaatccggc ataacccgcg accagcaccg cggcgggaat acccgcggct tcgcggacgc
      541 cttgccggaa gatggcctat tgcgcgcgca gacgcgggtc gagcgcgtcg cgcagcgagt
      601 caccgaatag gttgaagccg aacaccgcga ggctgatggc gattccggga aagatcgcca
      661 tccacggcgc gctctcggcg aactgcaccg ccgcgccgcg cagcatgagc ccccaggcgg
      721 cgaccggctc ctgcacgccc aggccgagga acgaaagcga agcctccagc aagatcgcct
      781 cgccgaggaa cgcggtcgcc atgatcagga tcggcgccat cacattcggc agcatatggc
      841 gcaggatgat gcggccgtcg ccgtaacccg aggcgcgcgc cgcatcgacg tacggcatct
      901 cgcgtaccgc gagcgcgctg gcgcggacca cgcgcgcgca gcgcggcacc atcggcacgg
      961 cgatggcaag gatcacgttg ctggcgccgg taccgagcac ggcgaccacc gccagcgcga
     1021 ggatgatgac tggaaaggcg aagaagacgt ccatgatgcg ctggacgatg aggtccacgc
     1081 ggccgccgaa ataggcgctc gccacgccga tcagcgagcc gagcgtggcg ccgagcagcg
     1141 atgcgcccag cccgaccatc agcgcggtgc gcgagccgta gatcaggcgc gaaagcacgt
     1201 cgcggccgaa ggcgtcggtg cccagccagt gctgagcatc gggcggcgcc agcatcgcgc
     1261 cgtagtcggt ctcgagcgga tggtggggag caagccagcc ggcagtcgcg gcgagcacga
     1321 ccatggcgag aatgatcgcc gcgccgatcg cgccgagcgg gcggcggcgc gtgaagtcga
     1381 gcgcaatgcc gagccagccg acgcgcgcgt cgtgccggct cagctcctca actgaagcgg
     1441 atgcggggat cgagccaggc ataggcgata tcgacgaaaa aattcacaag caggaatacg
     1501 gtggccacga gcatcaccag cgcctgcgtg agcgtgtagt cgcggcgcgc gatcgcctcg
     1561 acgaacagca ttcccagccc gttcaggttg aacacctgct cggtcacgac cagaccgccg
     1621 aggaggaacg cgaactcaag gctgatgatc gtgagcaccg gcaggatcgc gttcttcagc
     1681 gcgtggcgcg cgagaatcat attgggccag agtcccttgg cgcgcgcggt gcggatatag
     1741 tcctcgcgca gcacttcaag catcgccgag cgcatcatgc gcgtgcccac ggcggagtag
     1801 cggtaaccca cggcaagcgc gggccagatg agctgcgcca ggttctccca cggattcacc
     1861 cagaacggcg tgtagaccat cggcggaatc catttgaata ccgtcagcat cacgagaatc
     1921 atgaggatgc cgagccagaa cgacggcgtg gcgaggccgg cgatcgagaa tatgcgcacc
     1981 gcgtagtcga cccaggtgtc ctgcttgacc gcggcgagga tgccgagcgg gatggcgagc
     2041 agcgtggcga tggtgctcgc catgagcgcg agctgcagcg acagcgcgaa gcgcagcttg
     2101 atctcctcgg tgatcggtgc cccggtccac atcgacacgc cgaagtccag gcgcgcgagg
     2161 ccggccatcc agctcgtgaa ctgctgccac aggggcttgt ccagcccgag cttggcgcgc
     2221 tcctggttca tcagatcctg cgagaggaac tggctcccct gggcgaggta gcgggcctcg
     2281 actacgtcgc ccggcacgat gcgcaggaga ataaagatca gcaccgcgac ccccagcagc
     2341 gtcgggatca tcgcggcgag ccgcttgagg acgtaggcgc ccatcgtggc tcacgccggc
     2401 ggcatggtga ccgccggcgt gatgccgccg ctcagtcgcc gccttcaagc cagactgtgt
     2461 ccagagtgtt gttgaggtag tggctcggcg taatcgtcca gcccttgacc ttcgccgagt
     2521 gcgggatgat gcggtgccac tgcagggtca tcaggtagtg cacctcgtca tccagcaggc
     2581 gcttctcgaa ctgccgcacc agctgcttgc gctcctcggg gttggtcgcg cggctctgct
     2641 tgtcgtagag cgcgtcgagc accgggtctt tgtaacgcgc gtagttcgct gggttgcggt
     2701 cgacggaaag gaacttgtac atgtcgaggt cgggctcgac gatgtagccg cactgaaagt
     2761 ccatcgcgac ctggaagtcg cccttgcgca gcggcgtgta gtaggccgcg ggctcaagca
     2821 cctgctggtc gacgttcaag ccgacgcggc gccactggtc gatcagccac acgccgagcg
     2881 gctcgtaggg catggggata ccgcggttgg tgaaggtgaa cttgaagcct tcgggcacgc
     2941 cggcctcctt gagcagcctt ctcgcctcgg cgcgcgactt ctcgatatcg ggccagtagc
     3001 cggcgagctt gttcaactcc tccggcggcg tggcgaaggg cgtgccgggc acctgcactc
     3061 cggccacctc cttcacgatg gcaatcttgg aaagtgactt ggatgcctga tggcggtcga
     3121 gcgcgagcgt cagcgcgcgg cgcacgcgct tgtcgtcgaa gggcttctgc tcgtggttga
     3181 tcgcgacgag caggatgcag tcccacggcg attcctgcac tacgactttc ttacccagcg
     3241 tgctcacgat gctgtcgcgc tcggccgggg agaaaccacg gaattggatg tgcgcgcgct
     3301 cgccgcgcac cgcggcgacc tgcgccgcgg agtcgcgcat gaacacggcg cggaagccgt
     3361 tcaggtaggg cttgcccttg tcccagtagt cgggattctt tttgccgatc acatgcgagc
     3421 ccctgacgtg ctcagcgaat gtaaagggac cggtgcccat gatgttcttc tcgtaccagt
     3481 gagggtcctt ggccaggatg tccgccttgt agagaaagtt gtagggcgaa agcagggacg
     3541 agatgaacga gcctgagggc tccttcaggc ggaacacgac cgtggtcgcg tccggcgcgt
     3601 cgaccttctc cacgtcggcg tactggccct tgcgcgagga accgacgccg ggcggcggga
     3661 agatgatctt gtcgtaggtc gccttgacgt cgcgcgccgt gagctccgag ccatcatgga
     3721 acttcacgcc gcggcggatc ttgaaagtat aggtcttgcc gtcaggggac accgtccagc
     3781 tctcggcgac cgacggatga ggcttggtgc cgctcttgtc gttcgggtcg acgcgcagca
     3841 gcgtgttgta gaagggcgcg atcgggtgga tcaggccgaa ggtttcctcg cgatgcccgt
     3901 cgtacgacgg gggctccgag ggtacgggga acaccagttc accaccgtta cgttgggcct
     3961 gcagcggagc aggaccgaac agcgcgacac cggccgcgac ggccagcagg tatacgagtt
     4021 tcatggcgac tcctcctcga aggagtagag gccggtcgcg accgcctgtc aagcagctca
     4081 gcgccgggat cttaagtctg gcttaagcca acttgtgcaa ggtggcaggc ggcccagtgg
     4141 ccgggcctga tctccctgag cggcgggatc tctgcagtgc agcgctcgac cgcgatcgga
     4201 cagcgggggt ggaacacgca gcccgacggg ggcttgagcg ggctgggcac ttccccgcgc
     4261 agcacggtgc gttcgcgcgt cgcctccacg tcgggatccg ggatcggcac tgccgaaagc
     4321 agcgcgcggg tataggggtg cagcggttcc tcgtacagag ccgcgcggtc ggcgagctcg
     4381 acgatcctgc cgaggtacat cacggcgacg cggtccgaga tgtggcgcac caccgagagg
     4441 tcgtgggcga tgaagaggta ggtgagcccg aggcggctct gcaggtcctc gagcaggttg
     4501 atgatctgcg cctggatcga cacatcgagc gccgacaccg gctcgtcgca gacgatgagc
     4561 tcgggctcca tcgccagcgc gcgcgcgatg cccacgcgct ggcgctgccc gcccgagagc
     4621 tggtgcgggt agcgccgcgc gtgctgcggc agcagtccca cttgctcgag cagctcgcga
     4681 acgcgagcct cgcgcgccga acggctgcga atgatgccgt gcaccttgag cggctcggcg
     4741 agtatctggc cgaccgtcat gcgcggattg agcgaggagt atgggtcctg gaagatcacc
     4801 tggacgcgtc ggcgcaccgc gcgcagcgcc acggcgtcga tggcagcgag gtcgaccccg
     4861 tcgaagagga cgcggccgct cgtgggccgc tcgagctgca ggatgcagcg cccggtggtg
     4921 gttttgccac agcccgattc gccgaccagt ccgagggtct cgccgcgctt gagggtgaag
     4981 ctcacgccgt cgaccgcgcg caccgtgccg ccgggacccg cgaacgcgcc cccgcccaca
     5041 gggaaatgct tgacgaggtt gtcgacctcg agcagcgtgg tgctcaagcc gcgagctcct
     5101 tggcgaggcg catcgcttcc cagcacgcgg aggtatgcgg agcgcccgcc gcctggccta
     5161 cggcttcgag cggcggcact tcggcgcagc gcgcgacgcg ataggcgcag cgcggcgcga
     5221 aggcgcagcc cggcggcaac cgcgtgagat ccggcggctg tccctggatc ggatcgagcc
     5281 gtgcccggcg cggctcgtcc aggcgcggca ccgagcgcaa gaggccgagc gtatacgggt
     5341 ggcgcggatt ggcgtagagc tcgcgcgccg cgccctgctc gatgatgcgg ccggcgtaca
     5401 tcacgttcac ccggtcagcg tagcgcgcca cgacccccag attgtgagtg atgatcagca
     5461 tcgccacgcc gagccggcgc gcaaggtcgc gcatcagctc caggatttgc gcctggatgg
     5521 tgacgtcgag ggccgtggtc ggctcgtcgg ccaggatgag cgacggattg caggctagcg
     5581 ccatggcgat catcatgcgc tggcgcatgc cgccgctgaa gtgatgcgga tactgggcga
     5641 gccggcgttg cggatccggg atgccgacgg tcgcgagcag ctcgacggca cgttcgcgcg
     5701 cctgcgccgg gctcatcctt atgtggatct ccagcccctc ggtaagctgc cggccgatcg
     5761 aaagcaccgg gttgagcgaa gtcatcggct cctggaacac catcgcgatc tcgcgcccgc
     5821 gcacgcgctg catttcgggc tcggacagct tgagcaggtc gcgccccttg tagaggatct
     5881 cacccgcctc gatgcggccg gcgggctcgg caaccaggcg catcacgctt agcgcggaca
     5941 cggacttgcc gcagcccgat tcgccgacca gcgcgacggt ttcgccctcg cggatatccc
     6001 acgacacccc gtccacggcg cgcaccacgc cggcggaagt gaggaacgtc gtcctgaggt
     6061 tgcgaacttc gagcaggctg gccatggcgg ctaggctacc gcgtttgcgg cgaacagcgc
     6121 ctcgatctcc cgcgccgaga agccgaactc tccgagcacc tcgcgcgtat gctggccgaa
     6181 agtcggcgcc gggcgcgtca ccgcgcccgg tgtcgccgaa agct
//
LOCUS       DASUNT010000569         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_513925, whole genome shotgun sequence.
ACCESSION   DASUNT010000569 DASUNT010000000
VERSION     DASUNT010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513925"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFX94_11555"
     CDS             <1..195
                     /locus_tag="VFX94_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768862.1"
                     /translation="RGPTFETLTAVAFEPLARHARGKPEVLRSLLRAACALERAAPPR
                     RAAFVRNHIQVLQASLRECA"
     gene            362..1309
                     /locus_tag="VFX94_11560"
     CDS             362..1309
                     /locus_tag="VFX94_11560"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5768863.1"
                     /translation="MTRIAPIIVGFSLIAFAGAAGADDAKQQGASDRQQQGQQQGAAG
                     GSAQTSTSMSAKRLIGTDVVDAQGKDMGEIKEVVLDLHNGRVHAAVVEFGGFLGVGDK
                     QYAFPVSELKPAKERNKLMLNVSKDKLKDAQGFDKNKWPAMGDDYWGRVGGKAAAGGT
                     KAQGQKVNLHRASELMGQDVRNKDGQQVGEVSDIVLSSDRASIQHIVVKREGGGEARI
                     TPKQLSLGTDDKLVVDMPGAKAKQGSAKPGSAAGGASAASRTFGELDKDNDGALSQME
                     TAADANAKSNFAKLDKNNDKKLSRDEWQSGQAASGGASK"
     gene            1353..2318
                     /locus_tag="VFX94_11565"
     CDS             1353..2318
                     /locus_tag="VFX94_11565"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5768864.1"
                     /translation="MSSFARQALIVLAVVALAYLAWRVAYALLLAFGGILFALFLRGV
                     ADALARRTRLGMRVSLAVVGLVLLAGVALGALALGPQIAERFDELGRTLAQGAGEARA
                     YLRKTQWGQALLEQQGDLLGAASRVLITAVDAVLALVLVVVAGIYLAVSPRLYAEGVV
                     DLLPEQHRARGRDLLTSTGRALWRWVLGQFVTMAVVGMLTAAGLHLAGVPLAVPLGIV
                     AGLLEFIPFLGPFLAAVPIVLVALTVGTQTALYAALVLLVVQQLESNLLTPLVQRRAV
                     ALPPVLILVGTIALTLLFGALGAVFAAPLLVVVMVWTKMLYMKEA"
     gene            complement(2315..2536)
                     /locus_tag="VFX94_11570"
     CDS             complement(2315..2536)
                     /locus_tag="VFX94_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768865.1"
                     /translation="MSASEMVPALYYRLTDNWLELTVRFIAPTHAIRELKDAMSREIL
                     ARFDDAGLRVASATLEIVRYRTASGRRKR"
     gene            complement(2533..>2983)
                     /locus_tag="VFX94_11575"
     CDS             complement(2533..>2983)
                     /locus_tag="VFX94_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX5768866.1"
                     /translation="SIWFDDPTRLATALGLVTAGLAFALQKVITAVAGYFVILRGQTF
                     NVGDRITMGGVCGDVIALSFTQTTIMEMGQPPAVQNADPAMWVRSRQYTGRVVTVSNA
                     RVFDEPVFNYTREFPYHRFCSRRHAATRWSSAASAKRRSRSSSAAIS"
BASE COUNT          493 a          991 c         1035 g          464 t
ORIGIN      
        1 cgcggcccga cgttcgagac gctgaccgct gtcgccttcg agccgcttgc gcggcatgcg
       61 cgcggcaaac ccgaggtact ccgcagcctg ctgcgcgccg cgtgtgccct cgagcgcgcg
      121 gcgccgccgc gccgcgccgc gttcgtgcgc aaccacatcc aggtgctgca ggcgtctttg
      181 cgcgagtgcg catagctgcc gctgtcggaa gtacagcgag gaatgcaaac gcgcgcatgc
      241 ccaccacacg caatgcgccc aaaatggatg cccgcttcgg ttcttgtaac aagcccagcg
      301 ggcgggcgtt gggcacgatg cgtgcgtgca gttgtggaac ttcacttagg aggcacacga
      361 catgacacgc atcgctccca tcatcgtagg cttttctctt atcgccttcg ccggcgccgc
      421 aggcgcagat gacgccaagc aacaaggcgc cagcgaccgc cagcagcaag gccagcagca
      481 aggcgccgcc ggcggcagcg cgcagacgag cacctcgatg agcgcgaagc gcctcatcgg
      541 caccgacgtg gtcgacgccc agggcaagga catgggcgag atcaaggagg tcgtgctcga
      601 cctgcacaac ggccgcgtgc acgccgcggt ggtcgagttc ggcggcttcc tcggtgtcgg
      661 cgacaagcag tacgcgtttc ccgtaagcga actcaagccc gccaaggagc gcaacaagct
      721 catgctcaat gtcagcaagg acaaactgaa ggacgcgcaa ggctttgaca agaacaagtg
      781 gccggcgatg ggcgacgact actggggccg cgtcggcggc aaggccgccg ccgggggaac
      841 caaggcgcag ggacagaaag tcaacctgca ccgggcgagc gagctcatgg gccaggacgt
      901 gcggaacaag gacggccagc aggtgggcga ggtcagcgac atcgtgctca gctctgatcg
      961 cgccagcatc cagcacatcg tggtgaagcg ggaaggcggc ggcgaagcgc gtatcacgcc
     1021 caagcaactg tcgctcggca cggacgacaa gctggtggtt gacatgcccg gcgccaaggc
     1081 gaagcaaggc agcgcgaagc caggtagcgc cgccggtggc gcgagcgcgg caagcagaac
     1141 cttcggcgag ctcgacaagg acaacgatgg cgcactgagc cagatggaaa ccgcggccga
     1201 cgccaacgcc aagtccaatt tcgccaagct cgacaagaac aacgataaga agctctcgcg
     1261 cgacgagtgg cagtcgggcc aggccgcaag cggcggcgcc agcaagtgat gtcggccccg
     1321 gcagcgcgcc ggggcctcgc tgcagagtag gcgtgagcag tttcgcgcgc caggcgctga
     1381 tcgtgctcgc cgtcgtggcg ctcgcctatc tcgcctggcg cgtcgcctac gcgctcctcc
     1441 tcgccttcgg cggcatcctg ttcgcgctgt tcctgcgcgg ggtcgcagac gcgctcgcgc
     1501 ggcgcacgcg gcttggcatg cgggtatcgc tggccgtggt cggtctcgtc ctcctcgccg
     1561 gcgtggcgct cggcgcgctt gcgctcgggc cgcaaatcgc cgaacgcttc gacgagcttg
     1621 gccgcaccct cgcgcaaggc gcaggcgagg cgcgcgcgta tctgcggaag acgcaatggg
     1681 ggcaggcgct actggagcag cagggtgatc tcctgggggc ggcgagccgc gtgctaatca
     1741 cggcggtcga cgccgtgctc gcgctggtac tcgtggtggt cgccggcatc tatctcgccg
     1801 tgagcccgcg cctgtacgcc gaaggcgtgg tggacctctt gcccgagcag caccgcgcgc
     1861 gcgggcgcga cctgctgacg agcaccggca gagcactctg gcgatgggtt ctcggccagt
     1921 tcgtaaccat ggccgtagtc gggatgctca ccgccgccgg gcttcacctc gccggcgtgc
     1981 cgcttgccgt gccgctcggt attgtcgccg gtctgctcga gttcattccg ttcctggggc
     2041 cgttcctcgc cgcggtgccg atcgtcctcg tcgcgctcac cgtgggcacg cagacggcgc
     2101 tttatgcggc gctcgtcctg cttgtcgtgc aacagctcga gagcaacctg ctcacgccgc
     2161 tggtacagcg ccgggcggtc gcgctgccgc cggtgctgat cctcgtcggc acgattgccc
     2221 ttactctgct cttcggcgcg ctcggcgcgg tgttcgccgc gccgcttctc gtggtcgtga
     2281 tggtatggac caagatgctc tacatgaagg aggcctagcg tttgcgccgg ccgctcgcgg
     2341 tccggtaccg cacgatctcg agcgtggccg aggcgacgcg cagccccgcg tcgtcgaagc
     2401 gcgcgaggat ctcgcggctc atcgcgtcct taagctcgcg aatggcgtgg gtcggtgcga
     2461 tgaagcgcac ggtgagttcg agccaattgt cggtgagccg gtagtacagg gccggcacca
     2521 tctcgcttgc gctcacgaaa tagcggcgct cgagctccgc gagcgccgct tggccgaagc
     2581 tgccgagctc caccgtgtgg cggcgtgccg cctcgagcag aatctgtggt aagggaactc
     2641 gcgcgtgtag ttgaagaccg gttcgtcgaa aacgcgcgcg ttgctgaccg taaccacgcg
     2701 cccggtgtac tggcggctgc gcacccacat cgccgggtcg gcgttctgca ccgccggcgg
     2761 ctggcccatc tccatgatgg tggtctgcgt gaagctgagc gcgatgacat cgccgcacac
     2821 cccgcccatg gtgatgcggt cgccgacgtt gaaggtctgg ccgcgcagga tgacgaagta
     2881 gcccgccact gcggtgatca ccttctgcag ggcgaacgca agcccggcgg tgacgaggcc
     2941 gagcgcggtg gcaagccgtg tggggtcgtc gaaccagatc gac
//
LOCUS       DASUNT010000570         4825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1302854, whole genome shotgun sequence.
ACCESSION   DASUNT010000570 DASUNT010000000
VERSION     DASUNT010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302854"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VFX94_11580"
     CDS             complement(<1..354)
                     /locus_tag="VFX94_11580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX5768867.1"
                     /translation="MIRILAALLVSAPAFAHASYAERPEVEAFVRELVQRHGLGESEL
                     KRVFERAQRLEPVLEAIARPAERIRTWDEYRALMITERRIAEGVEFWNKNSRTLERAQ
                     RKYGVPPQYVVAIIGV"
     gene            complement(351..2306)
                     /locus_tag="VFX94_11585"
     CDS             complement(351..2306)
                     /locus_tag="VFX94_11585"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEX5768868.1"
                     /translation="MMHPLAPEQRQISGRDLAWLVASLLVVTAPHALRAPWWLTLLTL
                     CLYGWRLYFALNRGPLPARWLVLGVAAVAMLGVWVEFRTLFGRQPGILLLMLFSGLKL
                     LETRTHRDGAMAAFLGYFLVITNFLYAQTIPTAAAMCVGLFLLTATLVRLAAPQRAAP
                     ANLRTAGMLLVHAVPAALALFLLFPRVQGPLWGLPQDAYAGMTGLSDTMSPGTLSSVA
                     MSDAIAFRAEFHGASPPQTLRYWRGPVLWDFDGRSWSIGPSLLVDFAPPRGGIASYRY
                     DVLLEPHNRHWLFALETAASLPAGGARMSHDGQIVASAPVRTRTRYELYSVIAPQAQP
                     GEAAGPMRRALRLPEGSNPRSRTLAAQWRAASASDAEVVARAVGFLRQGGYAYTLEPP
                     LLGEHSVDDFLFSTKAGFCEHFASAFVFTMRAAGVPGRVVTGYQGGELNPVDSIITVR
                     QSDAHAWAEVLLPGQGWVRVDPTAAAVPGRLESGMARAVPQSAELPLLMRPELEWLRD
                     VRDRWEAVAHKWNVWVLGYNPERQRDLMASFGIRDADWRTLTATLFTFLGAMTLCLLA
                     WSLKRLARPDPVQRAWRTFCRKLALRGIERAPAEGPRDYAARAARALPAARRSILRIG
                     ALYIALRYGAQATPPAVTRLRKLVREF"
     gene            complement(2303..3271)
                     /locus_tag="VFX94_11590"
     CDS             complement(2303..3271)
                     /locus_tag="VFX94_11590"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5768869.1"
                     /translation="MIAGLIERNPRLANWLYGFTPPERGTVELVHRRVYIVPTRLGWF
                     FGGTLLVLLIGSINYALSLGFALTFLLAGLGLAGMVHTARNLAHIAVTAGRAEPVFAG
                     EPAQFRLWLDACASFDRPAILARHIASGSQLVVDIPARSTAEVLLAVPAPKRGRLALG
                     RVMLETRFPLGLFRAWSYVEPDARCVVYPRPERSPLPPFSGQAAAGALRTQTAGSDDF
                     AGLRGYQRSDSPRHIAWKAVARSEDLLTKQFAGESAAELWLDARLLPAGLALEQRLSR
                     LAGWVLAAERAGAHYGLRLSGVEIAPARGDVHRAACLQVLALCDTT"
     gene            complement(3268..3891)
                     /locus_tag="VFX94_11595"
     CDS             complement(3268..3891)
                     /locus_tag="VFX94_11595"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX5768870.1"
                     /translation="MRFVTCLLLAWPLATLGGAQIYEPLADNVRQRLSHMVADRAPAS
                     MHFREPGDGQRWLTEMDRRLERRIPDRQQRLELLRTVQYEATRAGLDPQLVLGVIEVE
                     SAFRKYAVSRAGARGYMQVMPFWVTLIGQPHHNLFHLRINIAYGCAILRHYLDMEKGD
                     YYRALGRYNGSLGRPEYPNLVLAAWRGRWKYEGPTAMIEHPAQARLT"
     gene            complement(3888..>4825)
                     /locus_tag="VFX94_11600"
     CDS             complement(3888..>4825)
                     /locus_tag="VFX94_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX5768871.1"
                     /translation="KVSTPGKVTCEEVAKLLQVPLQSTVKAVAVMHDQEFHLLLIRGD
                     HTVNEIKTQKLIGVFRFATDPEIERALGCEGGYIGPVGAKVSVIADRAVAVMSDFVCG
                     ANQEGFHLAGVNWGRDVAEPRVADIRNVVAGDPSPDGRGRLEIARGIEVGHIFQLRTK
                     YSEAMKASFLAEDGTARAFEMGCYGIGVTRIVAAAIEQNHDARGIVFPAPIAPFQASF
                     VPIGYHKNAQVRDAAERLYAQMLSAGIDVLMDDRDERPGVLFADMELVGIPHRVVLSE
                     RGLAAGNAEYKGRRDDKPQDVKLGSVVDFLKQRLA"
BASE COUNT          833 a         1628 c         1667 g          697 t
ORIGIN      
        1 tactccgatg atggccacga catactgcgg cggcacgccg tacttgcgct gcgcacgctc
       61 gagcgttcga ctgttcttgt tccagaactc cacgccctcc gcaatgcgcc gttcggtgat
      121 catgagcgca cggtactcgt cccacgtgcg gatgcgctcg gccggccgag cgatcgcctc
      181 cagcaccggc tcgaggcgct gggcgcgttc gaacacgcgt ttcaactcgc tttccccgag
      241 gccgtggcgc tggaccagct ccctcacgaa ggcttcgact tccgggcgct cggcatagga
      301 ggcatgggcg aacgccgggg ccgataccag gagcgcagcc aggatacgga tcaaaattcg
      361 cgcaccagtt tgcgcagccg cgtcactgcc ggcggggttg cctgcgcgcc atagcgcagc
      421 gcgatgtaga gagccccgat acgcaggatg ctgcgccgcg ccgcgggcag cgcgcgcgcg
      481 gcgcgcgcgg cgtagtcgcg cggaccttcc gcaggcgcgc gctcgatgcc ccgcaacgcg
      541 agcttgcgac agaaggtgcg ccacgcccgc tgcaccgggt ccgggcgcgc aaggcgcttg
      601 agcgaccagg cgagcaggca cagcgtcatc gccccgagga acgtgaagag cgtcgccgta
      661 agggtgcgcc agtcggcgtc gcggatgccg aagctcgcca tgagatcacg ctggcgctcg
      721 gggttgtagc ccagaaccca gacgttccac ttgtgcgcca cagcctccca gcgatcgcgc
      781 acgtcgcgca gccactcgag ctccggacgc atgagcagcg gcagctccgc ggactgcggc
      841 acggcgcgcg ccatgccgga ctccaagcgg cccggcaccg cggccgcggt cgggtcgacc
      901 cgtacccagc cttggccggg cagcaatacc tcggcccagg cgtgcgcgtc ggactggcgc
      961 acggtgatga tgctgtcgac cggattcaac tcgccgccct ggtagccggt gaccacgcgg
     1021 cccggaacgc cggcagcccg catcgtaaac acgaacgcgg aggcgaaatg ctcgcagaac
     1081 cccgccttgg tggagaagag aaaatcatcc acggagtgct cgccgagcag gggcggctcg
     1141 agcgtatagg cgtagccacc ctggcgcagg aagcctacgg cgcgcgctac cacttccgca
     1201 tcgctcgcag aggcggcgcg ccactgcgca gcaagcgtcc ttgagcgcgg attcgacccc
     1261 tcggggaggc gcagcgcgcg gcgcatcggc cccgccgctt cgccgggctg cgcctgcggc
     1321 gcaatgaccg agtacagctc atagcgcgtg cgcgtgcgta ccggcgcgct cgcgacgatc
     1381 tgtccgtcgt ggctcatgcg cgcgccgccc gcgggcaggc tcgctgcggt ttccaaggcg
     1441 aacagccagt ggcggttgtg gggctcgagc agaacatcgt agcgatacga ggcaattcct
     1501 ccccgcggcg gcgcgaaatc gaccagcaag ctcggcccga tgctccagct gcggccgtcg
     1561 aagtcccaca gcaccgggcc gcgccagtag cgcagggtct gcggcggcga ggcgccgtgg
     1621 aactcggcgc gaaatgcgat cgcatccgac atcgccacgc tggacagcgt ccccgggctc
     1681 atcgtgtccg agaggcccgt catgccggca tacgcatcct gcggcaggcc ccagagcggc
     1741 ccctgcacgc ggggaaaaag cagaaagagc gcgagcgctg ccgggacggc gtgcacgagc
     1801 agcatgcctg cggtgcgcag attggcgggt gcggcgcgct ggggtgccgc gagccggacg
     1861 agcgttgccg tcagcaggaa aaggccgacg cacatcgcgg ccgccgtggg aatggtctgc
     1921 gcgtagagaa aattggtgat cacgagaaag tagccgagaa aggccgccat cgcgccgtct
     1981 cggtgcgtgc gggtctcgag aagtttgagc cccgaaaaaa gcatcagcag cagaattccc
     2041 ggctgacggc cgaacagcgt gcggaattcc acccatactc cgagcatggc gaccgcggcg
     2101 acgccgagca ccagccagcg cgcgggcagg ggtccgcggt tcagagcgaa gtacaggcgc
     2161 cagccgtaca agcacagggt gagcagcgta agccaccatg gcgcgcgcag tgcgtgcggt
     2221 gcggtcacga caagaagcga ggcgaccagc caggcaagat cgcggccgct gatctgtcgc
     2281 tgctccggtg cgagcggatg catcatgtcg tgtcgcagag cgcgagcacc tgcaggcagg
     2341 cggcgcggtg cacgtcgccg cgcgcaggcg caatctcgac tccggacagg cgcagtccgt
     2401 agtgcgcgcc cgcgcgctcg gccgcaagca cccagcctgc gaggcgtgac agccgctgtt
     2461 ccagcgcgag gcccgcgggg agcagccgcg cgtcgagcca cagctcggct gccgactccc
     2521 cggcgaactg cttggtgagc aggtcctcgc tccttgcgac cgctttccag gcgatgtggc
     2581 gcggcgagtc ggaacgctgg tagccgcgca ggccggcaaa atcgtcgctt ccggctgttt
     2641 gcgtacgcag cgcgccggcc gcggcctgcc cgctgaaggg cggcagaggc gagcgctccg
     2701 ggcgcggata aacaacgcag cgggcgtcgg gctcgacata gctccaggcc cggaacaggc
     2761 cgagcggaaa gcgcgtctcc agcatcacgc ggccgagtgc gaggcgtccg cgcttgggcg
     2821 ctgggacagc gagcagcacc tcggccgtgc tgcgcgccgg gatgtcgacg acgagctgcg
     2881 agcccgaggc gatgtgccgg gcgaggattg cgggccggtc aaaagaagcg caagcgtcga
     2941 gccagagacg aaactgcgcc ggctcgcccg cgaatacggg ctccgcccgc ccggccgtca
     3001 cggcaatgtg tgccaggttg cgcgcggtat gcaccatgcc ggcgaggccg aggcccgcga
     3061 gcaggaaggt gagggcgaac ccgagcgaca aggcgtagtt gatcgagccg atgagcagga
     3121 cgagcagcgt gccgccgaag aaccacccga gccgcgtcgg cacgatgtac acgcgccggt
     3181 gcacgagctc caccgtgccg cgctcgggcg gggtgaagcc gtaaagccag ttggcgagcc
     3241 gcgggttgcg ctcgatcaga ccggcgatca cgtcaagcgc gcctgcgcag ggtgctcgat
     3301 catggccgtc ggtccctcgt atttccagcg tccgcgccat gcggcgagca ccaggttggg
     3361 atattcgggc ctgccgaggc tgccgttgta gcgaccgagc gcccggtaat agtcgccttt
     3421 ctccatgtcg aggtagtggc gcaggatcgc acagccgtac gcgatgttga tcctcaggtg
     3481 gaacaggttg tggtgcggct gcccgatgag cgtcacccag aacggcatca cctgcatgta
     3541 gccgcgcgcg ccggcacgcg aaacggcgta cttgcgaaaa gcgctctcca cttcgatgac
     3601 gccgagaacg agctgggggt cgagtccggc gcgcgttgcc tcgtactgca cggtgcgcag
     3661 gagctcgagc cgctgctggc ggtcaggaat gcgccgctcc aggcgtcggt ccatctccgt
     3721 cagccagcgc tggccgtcgc caggctcgcg aaaatgcatg gacgccggcg ctcgatcggc
     3781 caccatgtgg gaaagccgct ggcgcacgtt gtcggcgagc ggctcgtaga tctgagcgcc
     3841 gcccaacgtg gcgagcggcc aggcgaggag caggcaggtg acgaacctca cgccagccgt
     3901 tgcttgagga aatcgaccac cgaaccgagc ttcacgtcct gcggcttgtc gtcgcgacgg
     3961 cctttgtact cggcgttgcc ggcagcgagc ccgcgctcgg aaaggaccac gcgatgcggt
     4021 atgccgacga gctccatgtc ggcaaacagc actccggggc gctcgtcgcg atcgtccatg
     4081 agcacgtcga ttccagcgct cagcatctgc gcgtagagtc gctcggccgc atcgcggacc
     4141 tgtgcgttct tgtgatagcc gatcggcacg aaggatgcct ggaatggcgc aatgggcgcc
     4201 gggaacacga tgccgcgcgc atcgtggttc tgctcgatcg ccgcggctac gatgcgcgtc
     4261 acgccgatgc cgtagcagcc catctcgaac gctctggcag tgccgtcttc ggcgagaaag
     4321 ctcgccttca tagcctcgga gtacttggtg cgcagctgga aaatatgccc cacctcgatg
     4381 ccgcgcgcga tctcgagccg ccccctgccg tccgggctgg ggtcgcccgc taccacattg
     4441 cgaatatcgg cgacccgcgg ttcggcgaca tcccgacccc agttcacccc cgccaggtga
     4501 aaaccttcct ggttcgcacc gcacacgaag tcgctcatca ccgcgactgc ccggtcggcg
     4561 atcacgctta ctttggcgcc tacaggaccg atgtagccgc cctcgcatcc aagagcgcgt
     4621 tcgatctccg ggtccgtggc gaagcgaaaa acgccgatga gcttttgagt cttgatctcg
     4681 ttcacggtat ggtcgccgcg gatgagaagc aggtgaaact cctggtcatg catgaccgcg
     4741 accgccttca cggtgctctg tagcggcacc tgcaggagct tggccacttc ctcgcaggtg
     4801 accttgcctg gcgtggacac ttttt
//
LOCUS       DASUNT010000571         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1126543, whole genome shotgun sequence.
ACCESSION   DASUNT010000571 DASUNT010000000
VERSION     DASUNT010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126543"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VFX94_11605"
     CDS             <1..699
                     /locus_tag="VFX94_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5768872.1"
                     /translation="EHEGAYTVVGVLAKTGSVIDRLVLTPIESVWQVHEEHQGPQDDA
                     DRKALMEAREVTVVLVQYASPLAAAMLPRQINSQSELQAASPAYETARLFSIVGVGID
                     ALRGFAAVLILAAGLSVFIALYGALEERRYELAVMRTLGASPGKLFGLLIGEALVLSL
                     LGVVLGLMLGHALASILGAWLERQQHYPVTGLEWRPEELWVAGIALTVGVVAAIVPAW
                     RAYRTDVSRTLAQG"
     gene            701..940
                     /locus_tag="VFX94_11610"
     CDS             701..940
                     /locus_tag="VFX94_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768873.1"
                     /translation="MKTLLLLLGIGLSANLYAQGHGKKEEQQDAKRHRVMAAAHEAAA
                     KCLEAGKGEKVCLEQLLKDCKGVGIGKYCGMKHQH"
     gene            948..1415
                     /locus_tag="VFX94_11615"
     CDS             948..1415
                     /locus_tag="VFX94_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HEX5768874.1"
                     /translation="MNKILLAVLLAFAARAIAQHQVPPGVDPNQFKPLPERKDVVSWK
                     TLSQVELVKQKDRYVPQFAKDVSALDQKEVKVQGFMMPLQVGDKQSHFVLTAMPQSCA
                     FCLPGGPESMVEVKSKTPVKYTFDAVVLTGKLTVLKDDPTGIFYRLTDAIPSK"
     gene            1506..2039
                     /gene="hslV"
                     /locus_tag="VFX94_11620"
     CDS             1506..2039
                     /gene="hslV"
                     /locus_tag="VFX94_11620"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX5768875.1"
                     /translation="MEQLHGTTILSVRRGATVALGGDGQVTLGQVVLKGGARKVRRLY
                     QDRILAGFAGGTADAFTLFERFEAKLDKHQGNLMRAAVELAKDWRTDRMLRRLEAMLA
                     VADTEKSLIITGMGDVLEPEHGILAIGSGGPYAQAAARALLEHSTLAAPEIVKRSLEI
                     AGDICIYTNQSHTIEEL"
     gene            2042..>2255
                     /gene="hslU"
                     /locus_tag="VFX94_11625"
     CDS             2042..>2255
                     /gene="hslU"
                     /locus_tag="VFX94_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232231.1"
                     /note="heat shock protein involved in degradation of
                     misfolded proteins; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HEX5768876.1"
                     /translation="MSMTPREIVEELDKHIVGQNEAKRAVAIALRNRWRRAQIKEPLH
                     SEITPKNILMIGPTGVGKTEIARRLAK"
BASE COUNT          406 a          732 c          764 g          353 t
ORIGIN      
        1 gagcacgaag gggcgtacac cgtggtgggg gtgctggcga agaccggttc ggtgatcgac
       61 cggctggtgc tgaccccgat cgagagcgtg tggcaggtgc acgaggagca ccagggcccg
      121 caggacgatg ccgatcgcaa ggcgctgatg gaagcgcgcg aggtcaccgt ggtgctggtg
      181 cagtacgcct cgcccctcgc cgcggccatg ctgccgcgcc agatcaattc gcaaagcgag
      241 ctgcaggccg cttcgcccgc gtacgagacc gctcgcctgt tcagcatcgt cggcgtcggc
      301 atcgatgcgt tgcgcggctt cgcggcggtg ctgatcctcg ccgccggact ctccgttttt
      361 atcgcgctct atggcgcgct cgaggagcgc cgttacgagc tggcggtgat gcgcacgctc
      421 ggcgcttcgc ccggcaagct gttcgggctg ctgatcggcg aagcgctcgt gctgtcgctg
      481 ctcggcgtgg tgctcggcct gatgctgggc catgcgctcg cctcgatcct cggcgcgtgg
      541 ctcgagcgcc agcagcatta ccccgtcacg ggactggaat ggcgccccga agagctctgg
      601 gtcgccggaa tcgcgctgac cgtgggcgtg gtcgcagcca tcgtcccggc ctggcgcgcc
      661 tatcgaaccg atgtttctcg cacgcttgcg caggggtgac atgaaaacgc tgctgcttct
      721 actcggaata gggctttcgg ccaatctgta tgcccaggga cacggcaaga aggaagagca
      781 gcaggatgcc aagcgccacc gcgtgatggc cgcggcccac gaggccgccg ccaagtgcct
      841 ggaagccggc aagggcgaga aggtctgcct ggagcagctg ctgaaagatt gcaaaggcgt
      901 cggaatcggc aaatactgcg ggatgaagca ccagcactga ggatgtcatg aacaagattc
      961 tgttggccgt actgcttgcc ttcgccgcgc gggctatcgc ccagcaccag gtgccccccg
     1021 gtgtcgaccc gaaccagttc aagccgctgc ccgagcgcaa ggacgtggtg tcctggaaga
     1081 ccttgtcgca ggtggagctg gtgaagcaga aggatcgcta cgtgccgcag ttcgccaagg
     1141 acgtaagcgc gctcgaccag aaggaagtga aggtgcaggg cttcatgatg ccgctgcagg
     1201 tcggcgacaa gcagagccac ttcgtgctca ccgcgatgcc gcagagctgc gccttctgcc
     1261 tgccgggcgg ccccgagagc atggtcgaag tgaagagcaa gacgccggtg aagtacacct
     1321 tcgacgcggt ggtgctcacc ggcaagctca cggtgctcaa ggacgatccg accggcatct
     1381 tctaccgcct gacggacgca atcccgagca agtagttccc cggcgtttcg cgcgcgcaca
     1441 cgcagcgcgc gcgacataat cttatcttga cgttccggct gcgcatcccc acctagacga
     1501 ttcccatgga acaactgcac ggcaccacca ttctttcggt ccgccgcggc gcgacggtcg
     1561 cgctcggcgg cgacggacag gtcacgctcg gccaggtggt cctcaagggc ggcgcgcgca
     1621 aggtgcggcg cctataccag gaccgcatcc tcgccggctt cgccggcggc accgcggatg
     1681 ccttcaccct gttcgagcgc ttcgaggcga agctcgacaa gcaccagggc aacctgatgc
     1741 gcgccgcggt cgagctggcg aaggactggc gcaccgaccg catgctgcgc cgcctggagg
     1801 ccatgctcgc cgtggcggac acggagaaat cgctcatcat taccggcatg ggcgatgtgc
     1861 tcgagcccga gcacggcatc cttgccatcg gctcgggcgg gccttacgcg caggcggcgg
     1921 cgcgcgcgct gctggagcac agcacgctgg cggcgccgga gatcgtcaag cgatcgctcg
     1981 agatcgcggg cgacatctgc atctacacca accagtcgca cacgatcgaa gagctctagc
     2041 catgtcgatg acgccgcgcg aaatcgtcga ggagctcgac aagcacatcg tcggtcagaa
     2101 cgaggcgaag cgcgcggtcg cgatcgcgct gcgcaaccgc tggcgccgcg cacagatcaa
     2161 ggagccgctg cacagcgaga tcacgccgaa gaacatcctg atgatcgggc cgaccggggt
     2221 cggcaagacg gagatcgcgc gccgtctcgc gaagc
//
LOCUS       DASUNT010000572         3585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_950535, whole genome shotgun sequence.
ACCESSION   DASUNT010000572 DASUNT010000000
VERSION     DASUNT010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950535"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VFX94_11630"
     CDS             complement(<1..870)
                     /locus_tag="VFX94_11630"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX5768877.1"
                     /translation="MNLDIDCLRTFAHIAHTMSFSRAAEVVRRTQATVSEQINKLETQ
                     LGKRLFVRRKGRVLQLTLDGDRLLQYASRILQLNDEAYSSMSDQALAGFVRLGVPLDF
                     FGRNLTSWLARFKSLHPGVGLEVESEQSEQLLRRASRGELDLAFFKQAPGAGHGHVVV
                     REQLVWVTRQDALSSLAPPLPLILFPEGCAYRRFALSTLRGQGWPFDISFVSPSFDCL
                     RTAVVEGLGITVLARALVTPPLRVVAQRFELPPLPAVEVAYMYGAEETPPVVAELARF
                     LADSLANAGPPRLA"
     gene            996..1757
                     /locus_tag="VFX94_11635"
     CDS             996..1757
                     /locus_tag="VFX94_11635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX5768878.1"
                     /translation="MKLAGATAAAGAALAAAPAGAQAPMRSRFGGVKALAFDAYGTLF
                     DVFSVTALCEQLFPGKGTQIAQTWRFKQLQYSLMRSLMGRHRDFWGLTEDGLVWATKN
                     AKVELTPDRKKQLMDAYLSLAAFPDVKPGLEALKKQGLKLAILSNGEPKMLDAAARSA
                     GIRELLDEIVSVEEVKIFKTSHRVYWLATERLHVSNAELGFVSANNWDGVAAVSAGLR
                     TFWIQRSAAEVPEELGFTVDTTVKGITDLPALLRA"
     gene            1950..2225
                     /locus_tag="VFX94_11640"
     CDS             1950..2225
                     /locus_tag="VFX94_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HEX5768879.1"
                     /translation="MNTNLTLAALAAALLAAGPALAQDKGASIKGPKEKCYGISLAGK
                     NDCAAGPGTTCAGTSTVDYQGNAWTYVPKGTCASIKTPRGTGSLKPV"
     gene            2246..3088
                     /locus_tag="VFX94_11645"
     CDS             2246..3088
                     /locus_tag="VFX94_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEX5768880.1"
                     /translation="MTSTTAPAGLGLKPQHYVEALAAPADGLWFEVHPENYMVAGGPR
                     LGWLHAIRARHPVALHGVGLSLAADAEPDPAHLARLAALAHRVDPWLVSEHLAWSVWR
                     GAYYPDLLPFPRTAQSLARIAANVGRVQEALGRPIAIENPSHYIGLPDHEMDEIDFLC
                     ELARRTGCGMLLDVNNVYVSARNLGTSAEAYVDAFPGELVREVHLAGHTPDPALGHAL
                     LIDSHDAPVAPEVWSLYERLIARIGPRPTLIERDGNLPAFSALMAERARADALLQEPL
                     RKAA"
     gene            3088..>3585
                     /locus_tag="VFX94_11650"
     CDS             3088..>3585
                     /locus_tag="VFX94_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEX5768881.1"
                     /translation="MLARFQDRFVRTLLAPELPAPPAFAVYRNTVMKGCIDALQANYP
                     SVARLVGEEWFRAAAAVYARANPPRVPMLTEYGAGFAGFLESFPPAAELPYLPAVARL
                     DRFWTEAHSAPDEPALRRLNGRRLQLHAAARWGWFALPAYTIWSANREERGDGGEITW
                     RAQGAL"
BASE COUNT          596 a         1213 c         1213 g          563 t
ORIGIN      
        1 ggcgaggcgc ggcgggccgg cgttggccag gctgtccgcg agaaagcggg caagctcggc
       61 cacgacgggt ggcgtttcct cggccccgta catataagcc acttcgaccg ccggcagcgg
      121 cggcagctcg aagcgctgag cgaccactcg cagcggcggt gtcacgagcg cgcgtgcgag
      181 cacggtgatg cccaggcctt cgaccacggc cgtcctcagg cagtcgaagc tcgggctgac
      241 gaagctgatg tcaaaaggcc agccctggcc gcgcagcgtc gagagcgcga agcgccggta
      301 ggcgcagcct tcgggaaaga ggatgagcgg cagcggggga gcgagcgacg acaaagcatc
      361 ctgccgcgtc acccagacga gctgctcgcg cacgacgacg tggccgtgcc cggcgccggg
      421 ggcctgcttg aagaaggcga ggtctagctc gccgcggctc gcgcggcgca ggagctgctc
      481 ggactgctcg gattcgacct ccagcccgac cccagggtgc aggctcttga accgggcgag
      541 ccagctggtg aggttgcgcc cgaagaagtc gagcggcacg cccaggcgca cgaacccggc
      601 gagcgcctgg tcggacatcg acgaataggc ttcgtcgttc agctgcagga tgcggctcgc
      661 gtactgcagc agccggtcgc cgtcgagcgt gagctgcagc acgcgacctt tgcgacggac
      721 gaacaggcgc ttgccgagct gcgtctcgag cttgttgatc tgctcgctga cggtcgcctg
      781 ggtgcggcgc accacttcgg cggcacgcga gaagctcatg gtgtgcgcga tgtgcgcgaa
      841 ggtgcgcagg cagtcgatgt cgagattcat ggagcctcct cataatcgag catgccgata
      901 cccgttatcg gctgaatcga tttcctgttt atgcactccg ccggtagagt cgccgcgaac
      961 cggaggaaat catgtcgaac gatcgaaggg aattcatgaa gcttgccggc gccaccgcgg
     1021 cggccggcgc tgcgcttgca gcggcgccgg cgggtgccca ggcgccgatg cgcagccgtt
     1081 tcggtggggt caaggccctc gcattcgacg cctacggaac attgttcgat gtgttctcgg
     1141 tgaccgcgct ctgtgagcag ctcttccccg gcaaaggcac gcagatcgcg cagacctggc
     1201 gcttcaagca gctccagtac agcctgatgc gcagcctgat ggggcggcac cgcgatttct
     1261 ggggcctcac cgaggacggc ctggtgtggg cgaccaagaa cgcgaaggtc gagctcacgc
     1321 cggacaggaa gaagcagctg atggacgcct atttgagcct cgccgcgttc ccggacgtga
     1381 agcccggcct cgaggcgctc aagaagcagg gcctgaagct cgccatcctg tccaatggcg
     1441 agccgaaaat gctggatgcg gcggcgcgca gcgccggcat ccgcgaactg ctggacgaga
     1501 tcgtcagcgt cgaggaggtc aagatcttca agaccagcca tcgggtctac tggctcgcca
     1561 ccgagcgcct ccacgtcagc aacgccgagc tcggtttcgt ctcggccaac aattgggacg
     1621 gcgtcgccgc ggtatccgcg gggctcagga ccttctggat ccagcgcagc gccgcggaag
     1681 ttcctgagga gctcggcttt acggtcgata cgacggtcaa gggcatcacc gatctgccgg
     1741 cgctgctgcg cgcgtaggcg cggcccgtgc tacaggcttg agcgccgcgc aggcggcgcg
     1801 gttcataggc gcgcaatgcg catgtcggca cttggtcgga catgcgacct agtacttacg
     1861 cgcagccgcg atctgtaagt catccgacca cgccgcgacc aacgcgccgt agtcctcaat
     1921 ccaactttcc tcgagaagga gctgacgata tgaacaccaa cctcacactc gctgcactcg
     1981 ccgccgcgct gctcgccgcg ggtccggcgc tcgcccagga caagggcgcg tccatcaagg
     2041 gcccgaagga gaagtgctac ggcatttcgc tcgcgggcaa gaacgattgc gccgctggtc
     2101 cgggcaccac atgcgccgga acgagcacgg tcgactatca gggcaacgcc tggacgtatg
     2161 ttccgaaagg cacgtgcgcg tcgatcaaga cgccccgggg caccgggtcg ctcaagccgg
     2221 tctgagcgac aggctctacg ccgcaatgac ctcgaccacg gccccggccg ggctggggct
     2281 caagccccag cactacgtcg aagcgctcgc agccccggct gacgggctgt ggttcgaggt
     2341 tcatccggaa aactacatgg tggccggcgg cccgcggctt ggctggctgc acgccatccg
     2401 agcgcgccac ccggtcgcgc tgcacggcgt cgggctctcg ctcgcggccg acgccgagcc
     2461 cgatccagcg cacctcgcgc gcctcgcggc gctcgcgcat cgtgtcgatc cctggctcgt
     2521 ttccgagcat ctcgcgtggt cggtctggcg cggcgcctat tatcccgatc tcctgccctt
     2581 tccgcgcacg gcacagtcgc tcgcgcggat cgcggcgaac gtcggccgcg tgcaggaagc
     2641 gctcggccgg ccgatcgcta ttgagaatcc ctcgcactac atcgggctcc cggaccatga
     2701 aatggacgag atcgacttcc tgtgcgagct cgcccgccgc accggctgcg gaatgctgct
     2761 cgacgtcaac aacgtctatg tcagcgcgcg caatctcggg acatctgccg aggcttacgt
     2821 ggacgccttt ccgggcgagc tcgtgcggga ggtgcatctc gccggccaca cgccggaccc
     2881 ggcgctcggg cacgctttgc tgattgactc gcacgacgcc ccggtcgccc cggaggtatg
     2941 gtcgctctac gagcggctga tcgcccgcat cgggccgcgg ccgacactga tcgagcgcga
     3001 cggaaatctt ccggcttttt ccgccctgat ggccgagcgg gcacgcgccg acgcgctgct
     3061 gcaggagccg ctgaggaaag ccgcctgatg ctcgcgcgct tccaggaccg cttcgtacgc
     3121 acgctgctcg cgcccgagct gccagcgccg ccggcattcg ccgtctatcg caatacagtg
     3181 atgaagggct gcatcgacgc cctgcaggcg aactacccca gcgtcgcgag gctggtaggc
     3241 gaagaatggt tccgtgccgc ggcggcggtt tatgcgcgcg caaatccgcc gcgcgttcca
     3301 atgctgaccg agtacggcgc gggcttcgcc ggcttcctgg agtcgtttcc tccggcagcc
     3361 gagctgcctt atctgcccgc ggtcgcaaga ctcgatcgct tctggaccga agcgcacagc
     3421 gcgccggacg agcccgctct gcggcggctg aacggccggc gcctgcaact gcacgccgcg
     3481 gcgcggtggg gctggttcgc gcttcctgcc tacaccatct ggtcggccaa ccgggaagag
     3541 cgcggcgatg gcggcgagat tacctggcgc gcacaaggcg cgctc
//
LOCUS       DASUNT010000573         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_776032, whole genome shotgun sequence.
ACCESSION   DASUNT010000573 DASUNT010000000
VERSION     DASUNT010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776032"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /gene="fusA"
                     /locus_tag="VFX94_11655"
     CDS             <1..1421
                     /gene="fusA"
                     /locus_tag="VFX94_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX5768882.1"
                     /translation="KGEVSPEELHEPLERALREGHLIPVVFTSAKTGAGVTELLDVIV
                     KLLPNPTEGNPPSYVVTKAGNGQGTDLQPVPDPGKHALAHVFKIEIDPYIGRVAVFRV
                     HQGRLTAGSQLYIGEGRKPIKAGHLYLLRGKTQVEVHEAIPGDICAIAKIDDIQFDHI
                     LHDSADDANVHAKPLDLPMPVFGLAVQPKKRGDEQKVSDVLHKILAEDPCFRIEHNSQ
                     ANETVMRGLGEMHLKAVLEKMSSQYKLDLDTRPPSIPYRETIAVRAEGHSRHKKQTGG
                     AGQFGEVFLKVEPLPRGKGFEFVDSVKGGAIPNQFIPSVEKGVRAVLDSGFLAGYPLQ
                     DVRVIVYDGKSHPVDSKDVAFQSAGRKAFLDALAKARPIVLEPIVNVEIVAPEDRMGD
                     IAGELSGHRGQIKGSDSPRPGLVQITAQAPLSELEHFPARLKSLTAGHGSYSLEFSHY
                     DPAPPQLQQKLVAAHRPAAAEE"
     gene            1433..2209
                     /locus_tag="VFX94_11660"
     CDS             1433..2209
                     /locus_tag="VFX94_11660"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5768883.1"
                     /translation="MRQGRGAPPLVFVHGFLCSHEDWRHQLRFFSKTREVVACDLRAH
                     GKTPGRPQEVSIEHFAGDVVALANNLELPPFILVGHSMGCRVVLEANRIAPERVAGIV
                     LIDGSRAGSGDPDAAEAAARAQIETRGYKAWAEDLFRQMFFESTPASERLVQRAVRQS
                     AEIGAALWPRMARWDAATLEAALAAVRAPVLVIQSTTRNAQGQRASLQARQSTPWLDL
                     LKAKLKSVQIEILPGLGHFAQLEAPERVNRLIAEFSRSTG"
BASE COUNT          402 a          771 c          740 g          338 t
ORIGIN      
        1 agaagggcga ggtgagcccg gaagagctgc acgagccgct cgagcgggcg ctgcgcgaag
       61 ggcacctcat cccggtggtt ttcacctcgg cgaagaccgg cgccggcgtc acggaattgc
      121 tcgacgtgat cgtgaagctt cttccgaacc cgaccgaagg caacccgccc tcctatgtcg
      181 tcaccaaggc gggcaacggc cagggcacgg atctgcagcc ggtgcccgac ccgggcaagc
      241 acgcgctcgc gcacgtcttc aagatcgaga tcgacccgta tatcggccgc gtggcggtgt
      301 tccgcgtcca ccagggacgg cttaccgcgg gcagccagct ctacatcggc gaaggcagga
      361 agccgatcaa ggccgggcac ctctatctgc tgcgcggaaa gacgcaagtc gaggtgcacg
      421 aggcgattcc cggcgacatc tgcgcgatcg ccaagatcga cgatatccag ttcgaccaca
      481 tcctgcacga ctccgccgac gacgcgaacg tgcacgccaa gccgctcgat ctgccgatgc
      541 cggtgttcgg cctcgcggtg cagccgaaaa agcgcggcga cgagcagaag gtgtccgacg
      601 tgctgcacaa gattctggcc gaggacccgt gcttccgcat cgagcacaac tcgcaggcga
      661 acgagaccgt gatgcgcggg ctgggcgaga tgcacctcaa ggcggtgctg gagaaaatgt
      721 cctcgcagta caagctcgac ctcgatacgc gcccgccctc gatcccgtac cgcgagacga
      781 tcgccgtcag ggccgagggc cacagtcgcc acaagaagca gaccggcggc gcgggccagt
      841 tcggcgaggt attcctcaag gtcgagccgc tgccgcgcgg caagggcttc gagttcgtcg
      901 acagcgtgaa gggcggcgcg atcccgaacc agttcatccc gtcggtggaa aaaggcgtgc
      961 gcgcggtgct cgattccggg ttcctcgccg gttatccact gcaggacgtg cgcgtgatcg
     1021 tgtacgacgg caagagccac ccggtggact ccaaggacgt cgccttccag tctgccggcc
     1081 gcaaggcgtt cctcgacgcg ctcgccaagg cgcggccgat cgtgctcgag ccgatcgtca
     1141 acgtcgagat cgtggcgccg gaagaccgca tgggcgacat cgccggagag ctctcgggcc
     1201 accgcggcca gatcaagggc tcggactcgc cgcgaccggg cctcgtccag atcaccgcgc
     1261 aggcgccgct ttcggagctc gagcacttcc cggcgcgctt gaagtcgctc accgcgggcc
     1321 acggctccta cagcctggag ttcagccact acgatccggc gccgccccag cttcagcaga
     1381 agctcgtcgc cgcgcacagg cctgccgccg ccgaggagta gagctgcact tcgtgcgcca
     1441 ggggcgaggc gccccgcccc tggtcttcgt gcacggtttt ctgtgcagcc acgaagactg
     1501 gcgccatcag ttacgttttt tctcgaaaac cagggaagtc gtggcctgcg acctgcgcgc
     1561 ccacggcaag acgccgggcc ggccgcagga agtctcgatc gagcatttcg ccggcgacgt
     1621 agttgctctt gcgaacaacc tcgaacttcc acctttcatt ttggttggcc atagcatggg
     1681 atgccgcgtg gtgctcgaag cgaaccgcat cgcgcccgag cgcgtcgccg gcatcgtgct
     1741 catcgacggc agccgcgccg gcagcggcga cccggacgcc gccgaggcgg cggcgcgcgc
     1801 gcagatcgag acacgcggct acaaggcctg ggcggaagat ctcttccggc agatgttctt
     1861 cgagtccacg ccggcctcag agcgcctcgt ccagcgcgcg gtgcgccaga gcgccgagat
     1921 cggcgcggcg ctatggccgc gcatggcgcg ctgggacgcc gcgaccctcg aagctgcgct
     1981 cgccgcggtg cgcgcgcccg tgctcgtcat ccaaagcacg acgcgcaacg cgcaaggcca
     2041 gcgcgcgtcg ctgcaggcga ggcagtcgac cccctggcta gaccttttga aggcgaaact
     2101 gaaaagcgta caaatcgaga tcctgcctgg tctcgggcat ttcgcgcagc tcgaggcccc
     2161 tgagcgcgtc aaccggctga tcgcggaatt ctctcgctcg accggttgaa acgccgcggc
     2221 ggcttgcgtc gtccaatgtg tgaccagact c
//
LOCUS       DASUNT010000574         2108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_864111, whole genome shotgun sequence.
ACCESSION   DASUNT010000574 DASUNT010000000
VERSION     DASUNT010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864111"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1814)
                     /locus_tag="VFX94_11665"
     CDS             complement(<1..1814)
                     /locus_tag="VFX94_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX5768884.1"
                     /translation="MIPELGQLALCLALAVALVQGIFPLMNPGAAALARSAAQAQALL
                     VAFAFGCLTYAFVTNDFSVQYVAMHSNSALPLHYRVSGVWGGHEGSFLLWCLMLAVWM
                     LAVAAFSAHLPEEMVARVLGVMGVLSAGFIAFMLFTSNPFDRLFPAPPDGRDLNPLLQ
                     DPGMVLHPPMLYMGYVGFSVAFAFAVAALLSGRLDAAWARWSRPWTTVAWVFLTLGIA
                     LGSGWAYYELGWGGWWFWDPVENASFMPWLVGTALIHSLAVTEKRGSFRSWTVLLAIV
                     AFGLSLLGTFLVRSGVLTSVHAFAVDPKRGIFILMLFALFIGGALALYVWRTSRIALG
                     GSFSAVSRESFLLANNVLLAAAAGTVFLGTLYPLAIDALGAGKISVGPPYFETVFVPL
                     VAPALFLLGIGPLARWKRASLPELAVRLRWALAVSLALGLLLPLVFGDWRPMVGFGLA
                     LAAWIVIAALLTIKGRQTRAHYGMVTAHIGVAVFVVGVTLVKAYEAERDANMKPGDSV
                     ALGAYAFRLDGISNVKGPNYVAARAAVSVTREGRPVKVLYPERRVYTVQEQVMTEAAI
                     APGFTGDLYVSLGDPLEGGAWLVKVQHKPFIDWIWGGCL"
     gene            complement(1811..>2108)
                     /locus_tag="VFX94_11670"
     CDS             complement(1811..>2108)
                     /locus_tag="VFX94_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX5768885.1"
                     /translation="APQGRTFRIGGMVEKGSVQRSGVEVRFLVTDTAKSIPVVYSGAL
                     PDLFREGKGVVAQGQLGSDGVFRAREVLAKHDENYMPPEAAHAVEKAQKTLQAK"
BASE COUNT          403 a          722 c          716 g          267 t
ORIGIN      
        1 aggcagccgc cccagatcca gtcgatgaag ggcttgtgct gcaccttgac gagccacgcg
       61 cccccttcga gcggatcgcc gagcgagacg tagagatcgc cggtgaatcc gggcgcgatc
      121 gccgcctcgg tcatcacctg ctcctgtacc gtgtagacgc ggcgctcggg gtagagcacc
      181 ttgaccggcc tgccttcgcg cgtgaccgag accgcagcac gcgccgcgac gtagttcggt
      241 cccttcacgt tcgagatgcc gtcgaggcgg aacgcgtacg cgccgagcgc gacgctgtcg
      301 ccgggcttca tgttggcatc gcgctccgcc tcgtaggctt tgacgagtgt cacgccgacc
      361 acgaacaccg ccacgccgat gtgggccgtg accatgccgt agtgcgcccg cgtctgtctt
      421 cctttgatcg tcagcagcgc cgcgatcacg atccaggcag cgagcgcaag cccgaagccg
      481 accatcggcc gccagtcgcc gaatacgagc ggcagcagca ggccgagcgc aaggctcacc
      541 gcgagcgccc agcgcaggcg caccgcgagc tcgggaaggc tcgcgcgctt ccagcgcgcg
      601 agcgggccga tgcccaggag gaaaagcgcc ggcgcgacga gcggcacgaa cacggtctcg
      661 aagtacggcg ggccgaccga gatcttgccg gcgccgagcg cgtcgatcgc gagcggataa
      721 agcgtcccga ggaacacggt gcccgccgcc gcggcgagca gcacgttgtt cgccagcagg
      781 aatgattccc tggatacggc agagaaggag cctcccagcg caatgcggga ggtgcgccag
      841 acgtaaagcg cgagcgcgcc gccgatgaag agcgcgaaga gcatcaggat gaagatgccg
      901 cgcttgggat ctacggcgaa ggcgtgcacc gaagtcagca cgccggaacg cacgaggaag
      961 gtgccgagga gactcagccc gaacgccacg atagcgagca gcacggtcca gctccggaag
     1021 ctgccgcgct tctcggtcac cgcgagcgaa tggatgagcg ccgtgccgac gagccagggc
     1081 atgaaggagg cgttttccac cgggtcccag aaccaccagc cgccccagcc gagctcgtag
     1141 taggcccagc cgctgccgag cgcgatgccg agagtaagga acacccaggc caccgtggtc
     1201 cagggacgcg accagcgcgc ccatgcggcg tcgagccggc ccgagagcag cgccgcgacc
     1261 gcgaacgcga aggcgaccga gaagccgacg taccccatgt agagcatcgg cggatgcagc
     1321 accatgccgg gatcctgcag cagcgggttc agatcacggc cgtcgggcgg cgccgggaac
     1381 aggcgatcga acgggttgga ggtgaagagc atgaaggcga tgaagcccgc gctcagcacg
     1441 cccatcacgc cgagcacgcg cgccaccatc tcctcgggca gatgagcgct gaaggccgcc
     1501 accgcgagca tccacaccgc gagcatcagg caccacagca ggaacgagcc ttcgtggccg
     1561 ccccagaccc ccgaaacgcg gtagtgcagc ggcagcgcgc tgttggaatg catcgccacg
     1621 tactgcaccg agaagtcgtt cgtcacgaaa gcgtaggtca ggcagccaaa ggcgaaagcg
     1681 acgagcagcg cctgcgcctg cgcggcactg cgggcgagcg cggccgctcc ggggttcatc
     1741 aacggaaaga ttccctgcac gagcgccacg gcaagggcaa ggcacagcgc gagttgtccg
     1801 agctccggga tcatttggct tgtaaggtct tctgcgcctt ttccaccgcg tgcgcggctt
     1861 ccggcggcat gtagttttca tcgtgcttgg cgagcacctc ccgcgcgcgg aacacgccgt
     1921 ccgagccgag ctgcccctgc gcgacgacgc ccttgccctc gcggaaaagg tcgggcagcg
     1981 cgccgctgta caccaccggg atgctctttg cggtgtcggt gaccaggaag cgcacctcga
     2041 cgccggagcg ctgcacgctg cctttctcga ccatgccgcc gatgcggaac gtgcggccct
     2101 gcggcgcc
//
LOCUS       DASUNT010000575         5933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1303856, whole genome shotgun sequence.
ACCESSION   DASUNT010000575 DASUNT010000000
VERSION     DASUNT010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303856"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VFX94_11675"
     CDS             <1..456
                     /locus_tag="VFX94_11675"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HEX5768886.1"
                     /translation="ELIQFRAAVESMAARLGAGVVAPEVRAALEAARELDRACADSDI
                     QVALHVVGQGIEADLGEQPFQVVRREDGVTLMLDVALTRDLGASYEAMTRAGRRLAET
                     YHARLVDDRDNLLDERSLAAIGAQLEAVRQTLAARGIETGSPLALRLFS"
     gene            453..2438
                     /gene="ligA"
                     /locus_tag="VFX94_11680"
     CDS             453..2438
                     /gene="ligA"
                     /locus_tag="VFX94_11680"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX5768887.1"
                     /translation="MTVERRIRKLRQAIERHNRLYYVEDAPEITDAEYDALFRELQAL
                     EAEHPELRCADSPTQRIGGAPLAEFGEVRHRTPMLSIANAFEEEEVRAFDRRVCQALG
                     TEVVEYAAEPKLDGLAVSLVYRDGAFVQGATRGDGTTGEDITANLRTIRSIPLAVKAT
                     YLEVRGEVLMYRSDFDALNKRQREAGQKEYVNPRNAAAGAVRQLDSRITASRPLRFFA
                     YAIANAERIDWKTQSQALERLAALGFAVSRERDVVDGAQGLLEYYARIGRVREKLPYA
                     IDGVVYKVNRLDWQERLGFVSRAPRFALAHKYPAEEQTTEVVGIEVQVGRTGALTPVA
                     KLKPVFVGGVTVTNATLHNESELRRKDVRVGDTVVVRRAGDVIPEVLSVRKELRPPNA
                     REFHMPEKCPVCGSAVVRNEEEAVHRCSGGLYCPAQRKQALLHFASRRAMNIEGLGER
                     LVDQLVDRGLVGNPADLYKLAAQDLESLERMGEKSAANLLRELERSRKASLERLIYAL
                     GIRNVGESTARDLARHFGDLQALSRATGEELAQVPDVGPIVARSIRQFFDEPHNRQVI
                     GDLVRRGVRWDKVAPAPGPAAQVKSFVLTGTLSAMTRDEARAAIEGKGHKVAGSVSKK
                     TDYVVVGQDAGSKLERARALGVPVLEEKEFIEMLQNL"
     gene            2442..3317
                     /gene="galU"
                     /locus_tag="VFX94_11685"
     CDS             2442..3317
                     /gene="galU"
                     /locus_tag="VFX94_11685"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX5768888.1"
                     /translation="MPAKLRKAVFPVAGLGSRFLPVTKASPKEMLPIVDKPLIQYAVE
                     EAAAAGITDMVFVTGRNKRAIEDHFDKAYEIETELERKNKSALLDMVRSVLPEGVRCM
                     YVRQAEPLGLGHAVLCAQPIVGDEAFAVLLADDLIDASPPAMCRMAEIFTETGASLLG
                     VEEVPRSQTQSYGIVTIDKMEADSARIHSIVEKPKPADAPSNLAVIGRYVLTPRIFDM
                     LAKVPPGAGGEIQLTDAISALLQHERVMAVRLPGKRYDCGSKLGYLQATLEYGLRHPE
                     TGEPFARYLKARGDA"
     gene            3310..3856
                     /locus_tag="VFX94_11690"
                     /pseudo
     CDS             3310..3856
                     /locus_tag="VFX94_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
     gene            3859..4596
                     /locus_tag="VFX94_11695"
     CDS             3859..4596
                     /locus_tag="VFX94_11695"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: protein motif:HMM:NF006599.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HEX5768889.1"
                     /translation="MLAIIGGSGLTKLANLEVGRRKVARTPYGDPSGPVTFGRIGDSE
                     VLFLARHGHGHTIAPHEVNYRANLWALKQAGADEVVSVASVGGIRNDIWPGTLLLPHQ
                     IIDYTWGRPATFFEGPGAPVNHIDFTEPYSRRMRERLLEAAAAAGERLTDGGVYAATQ
                     GPRLETAAEINRLEGDGADVVGMTGMPEAALAREIALEYAAIAVVANYAAGRGDSRRA
                     VALERIEAVLEEAMGRVRRIIEKLCEQ"
     gene            4593..5126
                     /gene="def"
                     /locus_tag="VFX94_11700"
     CDS             4593..5126
                     /gene="def"
                     /locus_tag="VFX94_11700"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX5768890.1"
                     /translation="MIRPVLRMGDPRLWQKSVPVEDFGTAELASLLVDMRDTMAHLNG
                     AGLAAPQIGVPLRVVIFGVSANPRYPDVEPVPDTVLINPVLTPLSSEEEEGWEGCLSV
                     PGMRGWVPRVRKLRYAGYDERGRRFQREVEGFHARVVQHEVDHLDGILYPMRIRDMTR
                     FGFNEELFPGAELPPQD"
     gene            5131..>5933
                     /locus_tag="VFX94_11705"
     CDS             5131..>5933
                     /locus_tag="VFX94_11705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEX5768891.1"
                     /translation="MARTLFEEEGEKVRLDRIPTAPPRGIDKQSARKRLEELGTELFE
                     LQDALWGSKANSVMVVLQGRDTAGKDGAIKNVAGYLNPRGVSVVSFGVPTEEERAHDF
                     LWRVHRHAPRLGEFAIFNRSHYEDVLVVRVHGLAPQRLWKERYGHIADFEELLAEHGT
                     IVLKFFLHISRKEQKKRLLERESDPRAAWKLNANDWKERERWNAYTEAYAEAISKTAA
                     PHAPWIVVPADAKWYRNLVVAETVVEALRKRRPHWKRALDEIARKRRAEL"
BASE COUNT         1003 a         1974 c         2099 g          857 t
ORIGIN      
        1 gagttgatcc agttccgcgc cgcggtcgaa agcatggcgg cgcggctcgg tgccggcgtg
       61 gtggcgcccg aggtgcgagc ggcgctcgag gccgcgcgcg agctcgaccg cgcgtgcgcc
      121 gacagcgata tccaggtggc gctccatgtc gtgggccaag gcatcgaggc cgacctgggc
      181 gagcagcctt tccaggtggt gcggcgcgag gacggcgtca cgctgatgct cgacgtggcg
      241 ctcacccgcg acctcggcgc gagctacgag gcgatgacgc gcgccgggcg ccgcctcgcc
      301 gagacgtacc atgcgcggct cgtcgacgac cgcgacaatc tgctcgacga gcgctcgctc
      361 gccgccatcg gagcgcaact ggaggccgtg cgccagacgc tcgccgcgcg cggcatcgag
      421 accgggagcc cgctcgcgct gcggctgttt tcatgacggt tgaaaggagg ataagaaaac
      481 tcaggcaagc aatcgagcgt cacaaccggc tgtactacgt cgaggacgcg cccgagatca
      541 ccgacgcgga gtacgacgcg ctgtttcgcg agctgcaggc gctcgaggcc gagcacccgg
      601 agctgcgctg cgcggactcg cccacgcagc gcatcggcgg cgcgccgctc gccgagttcg
      661 gcgaggtacg ccaccgtacg ccgatgctct cgatcgcgaa cgccttcgag gaggaggaag
      721 tgcgcgcctt cgaccggcgg gtatgccagg cgctcggcac cgaagtcgtc gagtatgcag
      781 ccgagccgaa gctcgacggg ctcgcggtga gcctggtgta tcgcgacggc gcctttgtcc
      841 agggcgccac gcgcggggac gggaccacgg gcgaagacat caccgcaaac ctgcgcacga
      901 tccgttccat tcctttagca gtaaaagcca cgtatctcga ggtgcgcggc gaagtgctga
      961 tgtatcggag cgacttcgac gcgctcaaca agcggcagcg ggaagccggc cagaaggagt
     1021 acgtgaaccc gcgcaacgcc gcggcgggtg cggtgcgcca gctcgattcg cgcatcacgg
     1081 cttcgcggcc gctgcgcttt ttcgcctacg ccatcgccaa tgccgagcgc attgactgga
     1141 agacgcagtc gcaggcgctc gagcggctcg ccgcgctcgg cttcgccgta tccagggagc
     1201 gcgacgtggt cgacggggcg cagggcctgc tcgagtatta cgcccggatc gggcgcgtgc
     1261 gcgagaagct gccgtacgcg atcgacggcg tggtgtacaa ggtgaaccgc ctggactggc
     1321 aggagcggct cggcttcgtc tcgcgcgcgc ccaggttcgc gctggcgcac aagtacccgg
     1381 ccgaggagca gaccaccgag gtggtcggga tcgaggtgca ggtcggccgc accggcgcgc
     1441 tcacccccgt ggccaagctg aagccggtat tcgtcggcgg ggtgaccgtg accaacgcca
     1501 ccttgcacaa cgagagcgag ttgcgccgca aggacgtgcg cgtcggcgac accgtagtgg
     1561 tgcggcgagc gggcgacgtc atccccgagg ttctctcggt aaggaaggaa ttgcgtccgc
     1621 cgaacgctcg ggagtttcac atgccggaga aatgcccggt atgcggctcg gccgtggtga
     1681 ggaacgagga ggaagccgtg catcgctgca gcggggggct gtactgcccg gcgcagcgca
     1741 agcaggcgct gctccacttc gccagccgcc gcgccatgaa catcgagggt ctgggagagc
     1801 ggctggtgga tcagctcgtg gatcgcggcc tcgtcggaaa tcccgccgac ctgtacaagc
     1861 ttgctgcgca agacctggag tcgctggagc ggatgggtga gaagtcggcc gccaatctcc
     1921 tgcgcgagct cgagcgctcg cgcaaggcgt cgctcgagcg gctgatctat gctttgggca
     1981 tccgcaacgt cggcgaaagc accgcgcgcg atctggcccg gcatttcggc gacctgcagg
     2041 ccctttcccg ggcaaccggg gaggagctcg cgcaggtacc cgacgtcggc ccgatcgtgg
     2101 cgcgctcgat ccggcaattc ttcgacgagc cgcacaaccg gcaggtgatc ggggacctcg
     2161 tccggcgcgg cgtacggtgg gacaaggtcg cgccggctcc cgggcccgcc gcgcaggtga
     2221 agagctttgt gctcacgggg accctctccg ccatgacgcg cgacgaggcg cgtgccgcga
     2281 tcgagggcaa ggggcacaag gtggctggct cggtgtcgaa gaagaccgat tacgtggtag
     2341 tggggcagga cgccgggtcc aagctggagc gggcgcgtgc gctgggggta ccggtactgg
     2401 aggaaaagga attcatcgaa atgctgcaga atctgtgacc catgcctgcc aagctcagaa
     2461 aagccgtatt cccggttgcc ggcctcggca gccgattcct gccggtgacc aaggcaagcc
     2521 cgaaggagat gctgccgatc gtcgacaagc cgctgattca atacgcggtc gaagaggccg
     2581 cggccgccgg catcaccgac atggtgttcg tcaccgggcg caacaagcgc gcgatcgaag
     2641 accacttcga caaggcctac gagatcgaga ccgagcttga gcgcaagaac aaaagcgcgc
     2701 tcctcgacat ggtgcgctcg gtgctgcccg agggagtgcg ctgcatgtat gtgcgccagg
     2761 ccgagccgct cggcctcggg catgccgtgc tgtgcgcgca gccgatcgtc ggcgacgagg
     2821 cgttcgccgt gctgctcgca gacgatctta tcgacgccag cccgccggcg atgtgccgca
     2881 tggcggagat cttcaccgag accggcgcct cgctcctcgg cgtcgaggag gtgccgcgca
     2941 gccagacgca gagctacggt atcgtgacga tcgacaagat ggaggcggac agcgcgcgca
     3001 tccacagcat cgtggagaag cccaagccgg ccgacgcccc gtccaacctc gcggtaatcg
     3061 gccgttacgt gctgacgccg aggatcttcg acatgctcgc caaggtgccg cccggcgcgg
     3121 gcggggagat ccagctcacc gacgcgatct ccgcgctgct ccagcacgag cgcgtgatgg
     3181 cggtgcgcct gccggggaag cgctacgact gcggctcgaa gctcggctac ctgcaggcaa
     3241 ccctcgagta cgggctgcgc catccggaga ccggcgagcc cttcgcgcgt tacctgaaag
     3301 cgcgcggcga tgcctgacgc agccgcggcc tgggcattcc tcgagaactc cgagctcgtc
     3361 gcgagcgccg aggaagtgcg ctctgccata cagcgcctcg cggccgaaat cgatcgcgcg
     3421 ctcggcgagc gctatccgct ggtgctcgcg gtcatgggag gcgcggtggt attcgccggc
     3481 cagatcctcc cgctgctacg ctttccgctg gacttcgact atgtgcacgc ctcgcgctac
     3541 ggcgcggcga cgcgcggcgc gcacgtcgag tggcgcgtgc ggccgcccgg gctcgtccgc
     3601 ggccgcaccg tgctggtgct cgacgacatt ctcgaccacg gcgagcacca tgagcgcgat
     3661 tcgcaagcgg ctcaccgagc tcggcgcgca aaccgtgtac gctgccgtgc tggtggagaa
     3721 gaatctccgg atcgcgaagc cggcgagcgc cgagttcgtc ggcttgcgca ttcccgagcg
     3781 cttcgtcttc ggctgcggca tggatgcccg gggcctgtgg cgcaatctcg ccgagatccg
     3841 ggccatgaga ccgtagcggt gctcgccatc atcggcggca gcggactgac caagctcgcc
     3901 aacctcgaag tcggccggcg caaagtcgcg cgcacgccct acggcgatcc ctccggcccc
     3961 gtgaccttcg gccgcatcgg cgactccgag gtcctttttc tcgcgcgcca cggccacggc
     4021 cacacgatcg cgccgcatga agtgaactac cgcgcgaact tgtgggcgct gaagcaggcc
     4081 ggggcggacg aggtggtgtc ggtcgcctcg gtcggcggca tccgcaacga catctggccg
     4141 gggacgctgc ttctgccgca ccagatcatc gactacacct ggggccggcc agccaccttc
     4201 ttcgaaggcc cgggcgcgcc cgtcaaccac atcgatttca ccgagccgta ctccaggcgc
     4261 atgcgcgagc ggctgctcga ggcggcggcc gccgcgggcg agcgcctcac cgacggcggc
     4321 gtgtatgccg cgacccaggg cccgcgcctc gagacggcgg ccgagatcaa ccggctggaa
     4381 ggcgacggcg ccgacgtggt cggaatgacc ggaatgccgg aagctgcgct cgcccgcgag
     4441 atcgcgctcg aatacgccgc catcgcggtg gtggcgaact acgccgccgg ccgcggcgac
     4501 agccggcgcg ccgtggccct cgagcgcatc gaagccgtgc tggaggaggc catgggccgc
     4561 gtgcggcgca tcatcgagaa gctctgcgag caatgatccg gccggttctg cgcatgggcg
     4621 atccccggct gtggcagaag tccgtgccgg tagaagactt tggaaccgca gaattggctt
     4681 cgttgctggt ggacatgcgc gacaccatgg cgcacctgaa cggcgcggga ctcgccgcac
     4741 cgcagatcgg ggtgccgctt cgcgtagtga tcttcggggt gagcgccaat ccgcgctatc
     4801 cggacgtcga gccggtgccc gacacggtcc tcatcaaccc ggtgctgacg ccgctctcga
     4861 gcgaggagga agaggggtgg gagggctgcc tttccgtgcc cgggatgcgc ggctgggtgc
     4921 cgcgcgtccg caagctccgc tacgcgggct acgacgagcg cggccgcaga tttcagcgcg
     4981 aggtcgaagg cttccacgcc cgggtcgtcc agcacgaggt ggaccacctt gacggtatcc
     5041 tgtacccgat gcgcatccgc gacatgacga ggttcgggtt caacgaggag ctgttcccgg
     5101 gcgccgagct gccgccgcaa gactagggcg atggcccgga cgctcttcga agaagaggga
     5161 gagaaggtcc gcctcgaccg gatcccgacc gcgccgcccc gcggaatcga caagcaaagc
     5221 gcgcgcaagc gcctggaaga gctcggcacg gagcttttcg agctgcagga cgcgctgtgg
     5281 ggctccaagg caaacagcgt gatggtcgtg ctgcagggcc gggacaccgc cggcaaggac
     5341 ggcgccatca agaacgtcgc gggctacttg aacccgcgcg gcgtaagcgt ggtgtccttc
     5401 ggcgtcccga ccgaggagga gcgcgcccat gactttctgt ggcgcgtgca ccgccacgcg
     5461 ccgcgcctgg gcgaattcgc gatcttcaac cgctcccatt acgaggacgt gctcgtggtg
     5521 cgcgtccatg gcctcgcgcc gcagcggctg tggaaagagc gctacggcca catcgccgac
     5581 ttcgaggagc tgctcgccga gcacggcacc atcgtgctca agtttttcct ccacatcagc
     5641 aggaaggagc agaagaagcg cctgctcgag cgcgagagcg acccgcgcgc ggcctggaaa
     5701 ctgaacgcca acgactggaa ggagcgcgag cgctggaacg cgtacaccga ggcctacgcg
     5761 gaagcgatct cgaagaccgc cgcgccgcat gcaccgtgga tcgtcgtgcc ggctgatgcc
     5821 aagtggtatc gcaacctcgt ggtcgcggaa acggtcgtcg aggccctgcg caagcggcgc
     5881 ccgcactgga agcgcgccct cgacgagatc gcgcgcaagc gccgcgccga gct
//
LOCUS       DASUNT010000576         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_951458, whole genome shotgun sequence.
ACCESSION   DASUNT010000576 DASUNT010000000
VERSION     DASUNT010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951458"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(532..2193)
                     /locus_tag="VFX94_11710"
     CDS             complement(532..2193)
                     /locus_tag="VFX94_11710"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5768892.1"
                     /translation="MTIETLIRGATVVDGTGARAKREDVGISGGKIVPPGPASKVINA
                     DGLVLAPGFIDPHTHYDAQICWDGATTPSSWHGVTTVVMGNCGVGIAPCRPAAREIAM
                     RDLVNVEAIPFEVLNAGITWDWESFPEYMDAAARRKPALNLAFLAPLTPFRHYVMGEA
                     SMERAATVEETQKISSLLDEAIGAGAFGWSSTLLNQHMGYGARPLACRNASREEVKAY
                     CNVLRERRKGAIEIAMTRQISVLEQAELDLLDFMLEQSRRPVTLIAMFDRDDLPEALR
                     TTMRKLEPMIARGARPQTSPLPLTRELVMRSPFAFAAFPSWKRLFIDTSKQAQAAVYR
                     DIAFRKQFREDLRQPASFADWARITLHEVNSSHLKKYEGKTVAEIALERGVDGVDALL
                     DITLEDDLENEFTVQSWNTRTERMAELLNDRSVLLGLGDGGAHLDMLCDSGYPTYVLG
                     TWVRERKVLSLEEAVRRMTSDPAEFFGIGDRGRIAPGMAADLVLFDPATVGMAARPER
                     MHDLPANGKRMVKRSRGIEMTMVNGVVTWEKSSLTGAAAGEVLRS"
BASE COUNT          386 a          814 c          766 g          446 t
ORIGIN      
        1 ggcgaagcgg gcgagctggg cgacgtgctg cgcttctggc tcgaacttcc agcggtgccg
       61 cctgtgcccg acgatccgct cgagctgccg ctgctcgcgc ccgtacctcc gcttccggca
      121 gtgtcggccg ggcggtcgca gcccgctagg agcgcgcccg aaagcgcgag ggaaagagcg
      181 agggtcagcg tgcgtgtgag tgtgcctgtc atgtgtggct ctcctttcgg acccggagcc
      241 aagcaacaag tagaccgagc cgcgggggcc gagcgcccgc cgcgcaagct tcggactgcg
      301 acagggatca aggtctgacg agacgcattc ccgctactcc gccacgcccg ccttcttgag
      361 gagctgccac accttctccg gcgtgatcgg aatgtcgacg tgggtgacgc cgaggtgcga
      421 cagcgcgtcc accacggcgt tggcaatcgc gggcggagcg ccgacggtcg ccggttcgcc
      481 ttgcagagag ctacgcgcag ctgcgggaaa aaccgttcct gacccctttc gtcatgagcg
      541 aaggacttcg cctgccgctg ctccagtaag gctgctcttc tcccaggtca ccacgccgtt
      601 caccatcgtc atctcgatcc cgcgcgagcg cttgaccatg cgcttgccgt tcgccggcag
      661 gtcgtgcatg cgctccgggc gcgcggccat gccgaccgtg gcggggtcga aaagcacgag
      721 atcggccgcc atgccgggcg cgatgcggcc gcgatcgccg atgccgaaaa actctgccgg
      781 gtccgaggtc atgcgccgca ctgcttcttc cagcgacagc actttccttt cgcgcaccca
      841 ggttccgagc acgtacgtcg gatagcccga gtcgcacagc atatcgagat gcgctccgcc
      901 gtcgcccagg ccaaggagca cgctgcggtc gttgagcagc tccgccatgc gctccgtgcg
      961 ggtgttccag gactgcacgg tgaactcgtt ctcgaggtcg tcttcgagcg tgatatcgag
     1021 cagcgcatcc acgccgtcca ctccgcgctc gagcgcgatc tcggcgacgg tcttgccttc
     1081 gtatttcttc aggtgggaag aattcacctc atgcagcgtg atccgtgccc agtcggcgaa
     1141 ggaggcgggc tgcctcaagt cttcacgaaa ctgctttctg aaagcaatgt ctcggtagac
     1201 ggcagcctgc gcttgcttcg aggtgtcgat gaaaagccgt ttccacgagg gaaacgcggc
     1261 gaaagcgaac gggctgcgca tcacgagctc ccgcgtgagc ggcagcgggg aggtctgcgg
     1321 ccgcgcgccc cgcgcgatca tcggctcgag cttgcgcatg gtggtgcgca gcgcttccgg
     1381 caggtcgtcg cggtcgaaca tcgcgatcag cgtcaccggg cggcggctct gctccagcat
     1441 gaaatcgagc aggtcgagct cggcctgctc gagcaccgag atctgccggg tcatcgcaat
     1501 ctcgattgcg cccttgcgcc gctcgcgcag cacgttgcag taggccttca cctcctcgcg
     1561 gctcgcgttg cggcacgcaa gcggtctcgc cccgtagccc atgtgctggt tgaggagcgt
     1621 gctggaccac ccgaacgccc cagccccgat cgcttcatcc aatagagaag aaattttctg
     1681 cgtctcctcg accgtcgcgg ctctctccat ggacgcctcg cccatgacgt agtgacgaaa
     1741 aggcgtcaaa ggcgccagga acgcgaggtt gagggcgggc ttgcggcgcg cggcggcatc
     1801 catgtactcg gggaagcttt cccagtccca ggtaatgccg gcgtttaaca cctcgaacgg
     1861 aatcgcttcc acgttcacga gatcccgcat tgcgatctct cgtgccgccg gccggcacgg
     1921 cgcgatcccg acgccgcagt tccccatgac cacggttgtc acgccatgcc aggaagatgg
     1981 cgtcgtcgct ccgtcccaac agatctgcgc gtcgtagtgc gtgtgcggat cgatgaaacc
     2041 tggggcgagc acgagtccgt cggcattgat gactttgctg gctggtccag gcggcactat
     2101 tttcccgccc gagatgccga catcctctcg cttcgcccgc gcgcccgttc cgtccactac
     2161 ggttgcgccc ctgatgagcg tttcaattgt catgacggac gatcgtaaca gtactcccct
     2221 gtctggtaat gtcgttggct ctcaccggat ggcctgcctc gacttcgacg ccctgtatcg
     2281 cgcagagtcg cgccgggtat tcgcgacgct cgtgcgcctg ctcggcgact tcgcgcttgc
     2341 cgaagaggct ctctccgagg cgtttcgcgc ggctctcgag caatggccgc gcgaaggcgt
     2401 gcccgccaat cc
//
LOCUS       DASUNT010000577         2672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1215805, whole genome shotgun sequence.
ACCESSION   DASUNT010000577 DASUNT010000000
VERSION     DASUNT010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215805"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VFX94_11715"
     CDS             <1..1039
                     /locus_tag="VFX94_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5768893.1"
                     /translation="TLRREPIGVAGLIVPWNFPMVTTSWKVAPALAAGCTVVLKPSEV
                     APLNAFVFAEVVETAGLPKGVLNVVTGTGPKAGEALVKHPGVDMISFTGSTRAGKRIS
                     ELAAQSVKRVALELGGKSASVILEDADLATAVKGTLNGCYLNSGQTCTALTRMLVPEK
                     RYDEAARLAAELARSFTVGDPLAESTRLGPLTSLAQLERVRGYIKKGVDEGAELLAGG
                     AEPPEGAPAGGYYVRPTVFGKVRNDMTIAQEEIFGPVLSIIAYKDEEDAVRIANETPY
                     GLAGAVWSKDDAHAQRVARRIRAGQVDVNGGAFNLNAPFGGFKQSGHGREAGVYGLEE
                     FLEYKSLQLKG"
     gene            1050..>2672
                     /locus_tag="VFX94_11720"
     CDS             1050..>2672
                     /locus_tag="VFX94_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5768894.1"
                     /translation="MRLLRLVTGLVIASFVVGHFFNHALGVVSIEAMDRLRFALASWW
                     RSPVGTFLLYGSLLTHFALALASLYRRSTLRMPFWEAAQLALGLAIPPLLIGHIVGTR
                     LAWSFLGHSVDYERVVGLLWSSEWSALRQSLLLLIVWSHLCFGLHYWLRARPRYRDLQ
                     PLAFAIALLLPAAALSGFWSAGFYLWPSIENVGGMQKYNVDLAGMSGEQRALMANWRS
                     GLEWGFWVALAATLLARWLRTRIGATYRLRHASGRLITAPVGRSILEAIRDEGVPHAA
                     VCGGRARCTTCRVRVSEGLAHLPPPGRLEAQALSRIEAPVNVRLACQTRPTRDVAVTP
                     LLPPNVGPAGTRDARSGAHGRERPIVAMFVDLRDSTRLGEGRLPYDVVFIMNQFFGEM
                     NAALRATGGYYAQFRGDGLLALYGLESALDAACRAAVEGAAEMQDRIERLSRSLAADL
                     EQPLRIGIGIHAGVAIVGTMGPPEAPIYSAIGDMVNTAARLENMTKAYNCVLVISAEA
                     LKQAGIDAQGSVQHQVRVRGRNERVAVYAVNDP"
BASE COUNT          387 a          923 c          942 g          420 t
ORIGIN      
        1 gacgctgagg cgcgagccga tcggtgtcgc gggcctcatc gtgccgtgga acttcccgat
       61 ggtgacgaca tcgtggaaag tggcgccggc gctcgccgcc ggctgcacgg tggtgctcaa
      121 gccttccgag gtcgcgccgc tcaacgcctt cgtcttcgcc gaagtggtcg agacggcggg
      181 cctgcccaag ggcgtgctga atgtcgtcac gggcaccggc ccgaaggcgg gcgaggcgct
      241 cgtcaagcac ccgggcgtgg acatgatttc attcaccgga tccacgcgag ccggcaagcg
      301 catatccgag ctcgccgcac aatcggtgaa gagagtcgcg cttgaactgg gcggcaagag
      361 cgcctccgtg atcctggagg acgccgatct cgccacggcg gtcaagggca cgctgaacgg
      421 ctgctacctc aattccggcc agacgtgcac ggcgttgacc cggatgctgg ttccggaaaa
      481 aaggtatgac gaagcagccc ggctggcggc cgagctcgcg aggagcttca ccgtcggcga
      541 tccgctcgcc gagtcaaccc ggctcgggcc gctcacttcg ctcgcgcagc tcgagcgggt
      601 gcgcggctac atcaagaagg gcgtcgatga aggcgccgag ctcctcgccg gcggggccga
      661 gccgccggaa ggcgcgcccg cgggcggcta ctacgtgcgc ccgaccgtct tcggcaaggt
      721 gcgcaacgac atgacgatcg cgcaggagga gatcttcggg ccggtgctct ccatcattgc
      781 gtacaaggac gaggaagatg ccgtgcgcat tgcgaacgag accccctacg ggctcgcggg
      841 cgcggtgtgg tcgaaggatg acgcgcacgc gcagcgcgtg gcgcgccgca tccgcgccgg
      901 ccaggtggac gtgaacggcg gcgctttcaa ccttaatgcc ccgttcggcg gattcaagca
      961 gtccggccat ggccgcgagg cgggcgtgta cggcctcgag gaatttctgg aatataagtc
     1021 cctgcagttg aagggataga cgccgcggga tgagactcct gcgcctcgtc acggggctgg
     1081 tgattgccag cttcgtggtc gggcatttct tcaaccacgc gttgggcgtg gtgtcgatcg
     1141 aggcgatgga ccggttgcgc ttcgcgctcg cctcgtggtg gcgcagcccg gtgggcacgt
     1201 tcctcctcta cgggtcgctg ctcacccatt tcgccctggc gctcgcgagc ctttaccggc
     1261 gaagcacgct gcgcatgccc ttctgggagg cggcgcagct cgcgctcggg ctcgccattc
     1321 cgccgctcct catagggcac atcgtcggca cgcggctcgc ctggagcttc ctcgggcaca
     1381 gcgtcgacta cgagcgcgtg gtcggcctgc tctggtcgag cgagtggtcg gcgctgcgcc
     1441 agtcgctgct tctcctcatc gtctggtcgc acctgtgctt cgggctgcac tactggctgc
     1501 gcgcgcggcc gcggtaccgc gacctgcagc cgctcgcctt cgccattgcg ctgctcctgc
     1561 ccgcggctgc gctgtcgggc ttctggagcg cgggcttcta cctctggccg agcatcgaga
     1621 acgtcggtgg catgcaaaag tacaacgtcg accttgccgg catgtcgggg gagcagcgcg
     1681 cgctcatggc gaactggcgc tcggggctcg agtggggctt ctgggtcgcg ctcgcggcta
     1741 cgctcctcgc gcgctggctg cgcacgcgca tcggcgcgac ctacaggctg cgccacgcct
     1801 ccggccgcct gatcaccgcg ccggtcgggc gctcgatcct cgaagcgatc cgcgacgagg
     1861 gcgtgccgca cgcggcggtc tgcggcgggc gcgcgcgctg caccacctgc cgcgtgcgcg
     1921 tgagcgaggg gctcgcccac ctgccgccgc ccgggcgcct cgaagcgcag gcgctctcgc
     1981 gcatcgaagc cccggtcaac gtgcgcctcg cctgccagac gcgcccgacg cgcgacgtcg
     2041 ccgtgacgcc gcttctgccc cccaacgtcg ggcccgccgg gacgcgcgat gcgcgcagcg
     2101 gcgcgcatgg gcgcgagcgc ccgatcgtcg cgatgttcgt cgacctgcgc gactcgacgc
     2161 gcctgggcga aggacgcctg ccctacgacg tggtcttcat catgaaccag tttttcgggg
     2221 agatgaacgc cgcgctgcgc gccaccggcg gctactacgc gcagttccgc ggcgacgggc
     2281 tgctcgcgct ttacggactg gaaagcgcgc tcgacgcggc ctgccgcgcg gcggtcgaag
     2341 gcgccgccga gatgcaagac cgtatcgagc ggctcagccg cagcctcgcg gccgatctgg
     2401 agcagccgct acgcatcggc atcggcatcc atgccggcgt cgccatcgtg ggcaccatgg
     2461 ggccgccgga ggcgccgatc tactcggcga tcggcgacat ggtgaacacc gccgcgcgcc
     2521 tggagaacat gaccaaggcc tacaactgcg tgctcgtcat ctcggccgaa gcgctgaagc
     2581 aggccggtat cgatgcgcag gggtccgtcc agcaccaggt ccgggtgcgc gggagaaacg
     2641 agcgcgtggc ggtctacgcc gtcaacgacc cg
//
LOCUS       DASUNT010000578         2086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1127801, whole genome shotgun sequence.
ACCESSION   DASUNT010000578 DASUNT010000000
VERSION     DASUNT010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127801"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VFX94_11725"
     CDS             complement(<1..465)
                     /locus_tag="VFX94_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003273512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5768895.1"
                     /translation="MQIRNSIFLVTGGASGLGAASARMAAEGGAKVVIADMQADAGEK
                     LAKELGGRFLKTDVSSEADAKAAVQLALKEFGGLHVLVNCAGIAIAERTLGKEAPHDL
                     QRFIRVLNVNVVGTFNMIRLAAEAMAKAVPNASGERGVIVNTASVAAFDGQIG"
     gene            505..>2086
                     /locus_tag="VFX94_11730"
     CDS             505..>2086
                     /locus_tag="VFX94_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768896.1"
                     /translation="MQPGFGIRFNDLYEREGLLRVDAAFLAFLSEGNTALHGRLLAFR
                     NAPPKAKEESDLLVALAPHVEDFLAKLFGIEDEARALAERHNELAPLYSVKRLFVQRR
                     ALHKVKPEDARPDGFHFTTELDFARQVSAWLKDEASHQTQLEGAARYAAWAALTPEGK
                     AKHRGGVLFKAPRKLDYLKLIPHTEVRHREGFKLTDPGTDLAGALDEANYCIWCHEQG
                     KDSCSKGLREKPPAEGFRKTVFGVQLAGCPLEEKISEFHMVKARGEPLAALGIIALDN
                     PMAAGTGHRICNDCMKSCIYQKQDPVDIPQAETRTLKDVLELPWGFEIYSLLTRWNPF
                     DLRRPYPKAPSGKRILVVGLGPAGFTVAHHLMNDGHTVVGIDGLKIEPLEAAICGVDP
                     LGNRVPFRPVKHVSELWEPLDERVMAGFGGVAEYGITVRWDKNFLKVIRLLLERRRQF
                     AMFGGVRFGGTLTIDEAFADGFDHIALCAGAGRPTVLEIPNGFARGVRAASDFLMALQ
                     LTGAAKRESIANLQIRLPI"
BASE COUNT          362 a          713 c          663 g          348 t
ORIGIN      
        1 gccgatctgc ccgtcgaagg cggcgaccga agccgtgttc acgatgacgc cgcgctcgcc
       61 ggaggcattc ggtaccgcct tcgccatcgc ctcggccgcg aggcggatca tgttgaacgt
      121 gccgactacg ttgacgttca gcacgcggat gaagcgctgc aggtcgtgcg gcgcctcctt
      181 gccgagcgtg cgctcggcga tcgcgatgcc cgcgcagttc acgagaacat gcagcccgcc
      241 gaactccttc agcgcgagct gcacggcggc cttcgcgtcc gcctcgctcg acacgtcggt
      301 cttcaggaag cgcccgccga gctccttggc gagcttctcg ccggcatcgg cctgcatgtc
      361 ggcaatgacg actttcgcgc cgccctcggc ggccatgcgc gcgctggccg cgcccagacc
      421 ggaagcgccg ccggtgacga ggaatatgct atttcggatt tgcatcggga actccgcatt
      481 gaattcgccg ataattctaa ccgcatgcaa cccggtttcg gcattcgttt caacgaccta
      541 tacgagcgcg aaggcctgct gcgcgtcgac gccgcttttc tcgcttttct ctccgagggc
      601 aacacggccc tgcacgggag gcttctggcc ttccgcaacg cgccgccaaa ggcaaaagaa
      661 gaatcggacc tgctggttgc acttgcgcca cacgtcgagg actttcttgc gaagctcttc
      721 ggcatcgaag atgaggcgcg cgcgctcgcc gagcggcaca acgagctcgc gccgctgtat
      781 tcggtaaagc gcctcttcgt tcaacggcgc gcgctgcaca aggtgaagcc ggaggatgcc
      841 agacccgacg gctttcactt caccaccgag ctcgacttcg cgcggcaggt gagcgcctgg
      901 ctcaaggacg aagcttcgca ccagacgcag ctcgagggcg ctgcccgcta cgccgcatgg
      961 gcggcgctca cgcccgaagg caaggcaaag caccgtggcg gcgttctgtt caaggcgccg
     1021 cgcaagctcg actacctgaa gctcattccg cacaccgagg tgcggcatcg ggaaggcttc
     1081 aagctcaccg acccgggcac cgacctcgcc ggcgcgctcg acgaggcgaa ctactgcatc
     1141 tggtgccacg agcaaggcaa ggattcgtgc tcgaagggcc tgagggaaaa gccgcccgcc
     1201 gagggctttc gcaagactgt attcggcgtg cagctcgcgg gctgcccgct cgaggaaaag
     1261 atctcggaat tccacatggt caaggcgcgc ggtgagccgc tcgctgcgct cggcatcatc
     1321 gcgctcgaca atccgatggc cgcgggcacc ggccacagga tctgcaacga ctgcatgaag
     1381 tcctgcatct accagaagca ggacccggtc gacattccgc aggcggagac gcgcacgctg
     1441 aaggacgtgc tcgagctgcc gtggggattc gagatctact cgctgctcac gcgctggaat
     1501 cccttcgatc tcaggcgccc gtacccgaaa gccccttcag gaaagagaat tctcgtcgtc
     1561 ggcctcggcc ccgcgggctt caccgtcgcc caccacctca tgaacgacgg ccacacggtc
     1621 gtcggaatcg acggcttgaa gatcgagccg ctggaggcgg caatctgcgg cgtcgatcct
     1681 ctcggaaatc gcgtcccctt ccggccggtc aagcacgtga gcgagctttg ggagccgctc
     1741 gacgagcgcg tgatggcggg cttcggcggc gtggccgaat acggtatcac cgtgcgctgg
     1801 gacaagaact tcctgaaggt gattcgcctg ctcctcgagc ggcggcggca gttcgccatg
     1861 ttcggcggcg tgcgcttcgg cggcacgctg acgatcgacg aggcgttcgc cgacggcttc
     1921 gaccacatcg cactgtgcgc gggcgcaggc cgcccgacgg tgctcgagat ccccaacggc
     1981 ttcgcgcgcg gcgtgcgcgc ggcctccgac ttcctgatgg ctctgcagct caccggcgcg
     2041 gccaagcgcg agtcgatcgc caacctgcag atccgcctgc cgatcg
//
LOCUS       DASUNT010000579         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_164782, whole genome shotgun sequence.
ACCESSION   DASUNT010000579 DASUNT010000000
VERSION     DASUNT010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164782"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="VFX94_11735"
     CDS             complement(<1..1199)
                     /locus_tag="VFX94_11735"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5768897.1"
                     /translation="MADVVFGNPFSAERAALVARLAPGAKLGDLTQDREALARLVAPR
                     LAPWLSRRLSDEERRLVEPALLYVSYHRFVPQIDAHIERQAKLGGAPLAVGFGEELVA
                     DLMGSGFPAEAAARYFAFFFQLRRAFYFIYGSLAGECESMRRLRESVWNNIFTHDMRG
                     FEAALWNRMEDFSTLILGETGTGKGQVAAAIGRSGFIPYLAAERRFAANFAESFIAIN
                     LSQFAESIIESELFGHRKGAFTGAIDHHEGVFERCNAHGALFLDEIGEVSIPIQIKLL
                     QVLQERTFTPLGGREKRRFSGRVIAATNQPLDRLRSEGRFRPDFFYRLCSDVIRMPTL
                     RERIAESSAEFPALVRLLVKRISGSTELADKVLESLPSGYAWPGNVRELEQAVRRVLV
                     TGRYVPE"
     gene            1371..1928
                     /locus_tag="VFX94_11740"
     CDS             1371..1928
                     /locus_tag="VFX94_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768898.1"
                     /translation="MRAVRIDSLAGALYLALSLGGCATTQEAKQLTSDHNPAPARQAE
                     DRPNPAGEVALKCTVGSLEMLRAGPLGVPLSIGAIGFVCLPLAIGAGVLHAAAPHLDL
                     GSGAVPDTGPVALRTGGSAELRALTTAERSVFPQSSAGGCTWGDCYAYKTSDTGSAGV
                     RKLSSHERAVFPTRFQAGCAAYGYW"
     gene            2004..2162
                     /locus_tag="VFX94_11745"
     CDS             2004..2162
                     /locus_tag="VFX94_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768899.1"
                     /translation="MNAIRLVGLACAMCLGVMLGLVFAGGALTVAVALIGGGGALTLG
                     VCAWAEWK"
BASE COUNT          379 a          705 c          734 g          367 t
ORIGIN      
        1 atttcgggaa cgtagcggcc ggtgacgagc acgcggcgta ccgcctgctc cagctcgcgg
       61 acgttccccg gccaggcata accgctcggc aggctctcga gcaccttgtc ggcgagctcg
      121 gtcgagccgg agatgcgttt cacgagcagc cgcacgagcg cggggaactc ggcgctcgac
      181 tcggcgatcc gctcgcgcaa ggtcggcatg cggattacgt ccgagcacag ccggtaaaag
      241 aaatccggcc gaaagcgccc ttcgctgcgc agccggtcga gcggctggtt ggtggcggcg
      301 atgacgcggc cggagaagcg cctcttctcg cgcccgccga gcggcgtgaa agtgcgctcc
      361 tggagcacct gcagcagctt gatctggatc ggtatggata cttcgccgat ctcgtcgagg
      421 aaaagcgcgc cgtgcgcgtt gcagcgttcg aacacgcctt cgtggtggtc gatcgcgccg
      481 gtgaacgcgc ccttgcgatg gccgaagagc tcggactcga tgatcgactc cgcgaactgc
      541 gacaggttga tcgcgatgaa gctctcggcg aagttcgcgg cgaagcgcct ttccgccgcg
      601 aggtagggaa tgaatcccga gcgcccgatg gcggcggcga cctggccttt cccggtgccg
      661 gtctcgccga ggatgagcgt cgagaagtcc tccatgcggt tccacagcgc cgcctcgaag
      721 ccgcgcatgt cgtgcgtgaa aatgttgttc cacaccgact cgcgcagccg ccgcatcgat
      781 tcgcactcgc ctgcgagcga gccgtagatg aagtagaagg cgcgccgcag ctggaagaag
      841 aacgcgaagt agcgcgccgc ggcctccgcg gggaagccgc tgcccatcag gtctgcgacc
      901 aattcttcgc cgaatccgac cgcgagcggc gcgccgccca gctttgcctg gcgctcgatg
      961 tgcgcgtcga tctgcggcac gaagcggtga tagctcacgt aaagcagcgc cggctcgacc
     1021 aggcgtcgct cttcatcgct cagcctgcgt gagagccacg gcgcgagccg cggcgccacc
     1081 agccgcgcca gcgcctcgcg gtcctgcgtc aggtcaccga gcttggcgcc tggagcgagc
     1141 ctcgcgacca gtgccgcgcg ctccgcgctg aaaggattgc cgaagacgac gtcggccaag
     1201 gcgcccagaa acgctcgatc tgacatgcat tcaatgtagt actgatctac aaggaatgct
     1261 agtgacgtag cgcctactgg ccttgactta tatgtgaatc aaatacttga gtcgtggcat
     1321 gccaggtgca tggctaggtc aagcgacaac caaaaatcaa gggagggcaa gtgagagcag
     1381 ttcgaatcga cagcctggca ggcgcgttgt acctcgcgct ctcgctcggc ggctgcgcga
     1441 ctacccaaga agcaaaacag cttacctcgg accataaccc cgcgcctgcg cgccaggccg
     1501 aggaccgtcc taatcccgcc ggcgaggtgg ccctgaagtg cacggtcggc tctctcgaga
     1561 tgcttagagc aggaccgctc ggcgtgccgc tttccatagg ggcgattggc ttcgtatgcc
     1621 tgccgctcgc gatcggcgcc ggcgtgctcc atgccgctgc accccatctc gacttgggga
     1681 gcggtgcggt tccggatacg ggcccggtcg cgctgcgcac gggtggcagc gccgagttgc
     1741 gcgcgctcac caccgccgaa aggtcggtgt ttccgcagag ctcggccggc ggatgcacct
     1801 ggggcgattg ctacgcctac aagacttcgg ataccggctc ggccggggtg cgcaagctca
     1861 gcagccacga aagggccgtt tttccgacgc gcttccaagc cggatgcgcg gcgtacggct
     1921 actggtagcc gcgcgccgcc tgtgagccat ccataagcga tccgtaatcg aactgcctgc
     1981 gcgcgcttcg caccatgccc tccttgaacg cgattcgact cgtcggtttg gcctgcgcca
     2041 tgtgcctggg ggtgatgctc gggctcgtgt ttgccggcgg cgcgctgaca gtcgcggtgg
     2101 cgctcatcgg aggcggcggg gcgctgacgc tcggagtgtg cgcgtgggcc gaatggaaat
     2161 gagcggaggc gaatcatgaa gactc
//
LOCUS       DASUNT010000580         2170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_428047, whole genome shotgun sequence.
ACCESSION   DASUNT010000580 DASUNT010000000
VERSION     DASUNT010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428047"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VFX94_11750"
     CDS             complement(<1..412)
                     /locus_tag="VFX94_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX5768900.1"
                     /translation="MNRYPTWGYALIALTIALGFLYTLPNFFGESPAVQVSSAKATVK
                     ADPALLQKVQDALQKAAISYTGAVLDPGGIRVRFADTDTQLKAKDLIHGTLNPGAAPQ
                     ATPEDDPNVNYVVALNLLPASPTWLSAIHALPMYL"
     gene            complement(409..744)
                     /gene="yajC"
                     /locus_tag="VFX94_11755"
     CDS             complement(409..744)
                     /gene="yajC"
                     /locus_tag="VFX94_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX5768901.1"
                     /translation="MESVVVISPAFAQAAPSGDSGYIGLLPIVLMFVLLYFLMIRPQM
                     KRAKEQKQMIEALQKGDEVITAGGVVGRITKLSDAYVSLEIAPNTEINVQKAAVQVLL
                     PKGTIKSIQ"
     gene            complement(764..1858)
                     /gene="tgt"
                     /locus_tag="VFX94_11760"
     CDS             complement(764..1858)
                     /gene="tgt"
                     /locus_tag="VFX94_11760"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX5768902.1"
                     /translation="MKFSLTRRDGAARRGVLELAHGRVDTPAFMPVGTYGTVKALSPE
                     EVAASGAQIVLGNAFHLWLRPGLEVIGKHGGLHRFMHWAGPILTDSGGFQVFSLETLR
                     KVTEEGVRFSSPVNGERLLLTPEQSVKIQQVLDSDIAMVFDECLAYPASEKRAAESMR
                     LSARWAARSKKAWHSANALFGIVQGGVYPQLRAESLDCLKEIGFDGYAIGGLAVGEPR
                     DERRKVLESIRLPEDRPRYLMGVGTPEDIIEAVAAGVDMLDCVLPTRNARNGWLFTRY
                     GDIRIRNARYRDDLAPLDERCACYTCRNYTRSYLYHLDKTKEMLGARLNTLHNLHYYQ
                     ELMRDIRLSIEENRFHVKAKALLEDRTRLL"
     gene            complement(1855..>2170)
                     /locus_tag="VFX94_11765"
     CDS             complement(1855..>2170)
                     /locus_tag="VFX94_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEX5768903.1"
                     /translation="PEGTFESLPGKRVLAVGTTSLRAMETAALTGRLEGETELFIYPG
                     FEFRVVKRLLTNFHLPRSTLLMLVAAFAGLDNIRRAYAHAIARRYRFFSYGDAMLIEG
                     RS"
BASE COUNT          371 a          669 c          747 g          383 t
ORIGIN      
        1 cgaggtacat gggcagcgca tggatggccg aaagccaggt gggcgatgcg ggcagcaggt
       61 tcaacgccac cacgtagttg acgttcgggt cgtcctcggg cgtcgcctgc ggcgcggcgc
      121 ccgggttcag ggtgccgtgg atcaggtctt tcgccttgag ttgcgtatcg gtgtcggcga
      181 agcgcacgcg gatgccgcca gggtcgagca ccgcgccggt gtaggagatg gcggccttct
      241 gcagcgcgtc ctgcaccttc tggagcagcg ccgggtcggc cttgaccgtc gccttggcgc
      301 tcgacacctg caccgccggc gactcgccga agaagttagg cagcgtgtag aggaagccga
      361 gcgcaatcgt gagcgcgatc agcgcgtagc cccaggtcgg gtatctattc actgaatgct
      421 tttgatagtg cctttaggga ggaggacctg gacggcggct ttctggacgt tgatctccgt
      481 gttcggcgcg atctcgaggc tgacgtaggc gtcggagagc ttggtgatgc gccctaccac
      541 gccgcccgca gtgatcacct cgtcgccctt ctgcagcgcc tcgatcatct gcttctgctc
      601 cttggcgcgc ttcatctgcg gccggatcat gaggaagtac agcagcacga acatcaggac
      661 gatcggcagc aggccgatgt atcccgagtc gccgctgggc gcagcctggg cgaacgctgg
      721 cgagatgacc actacggact ccagagggaa aaaacggcgc agattatagc agccgggtcc
      781 ggtcctccag cagcgccttt gccttcacgt ggaaacggtt ttcctcgatc gaaaggcgga
      841 tgtcgcgcat cagttcctga tagtagtgga ggttgtgcag cgtgttgagg cgcgcgccga
      901 gcatttcctt cgtcttgtcc agatggtaga gatacgagcg ggtgtagttg cggcacgtgt
      961 aacaggcgca gcgctcgtcg agcggcgcca ggtcgtcgcg gtagcgggcg ttgcggatgc
     1021 ggatgtcgcc gtagcgggtg aagagccagc cgttgcgcgc gttgcgggtg ggcagcacgc
     1081 agtccaacat gtcgacgcca gccgcgacgg cttcgatgat gtcctcgggc gtgcccacgc
     1141 ccatcaggta gcggggacgg tcctccggca ggcgaatgct ttcgagcacc ttgcgccgct
     1201 cgtctctcgg ctcgccgacc gcgagtccgc cgatcgcata gccgtcgaaa ccgatctctt
     1261 tcaggcaatc gagcgactcg gcacgcagct gcgggtacac gcccccctgc acgattccaa
     1321 acagcgcgtt cgcgctgtgc caggccttct tcgagcgcgc ggcccagcgg gcggagaggc
     1381 gcatcgactc ggcggccctt ttttcgctcg cggggtaggc gaggcactcg tcgaaaacca
     1441 tggcgatgtc ggagtcgagg acctgctgga tcttcaccga ttgctccggc gtgaggagca
     1501 ggcgctcccc gttcacgggc gaggagaagc gcacgccctc ttcggtcact ttgcgcagcg
     1561 tctccaggct gaacacctgg aagccgcccg aatcggtgag gatcggcccc gcccaatgca
     1621 tgaagcgatg cagcccgccg tgcttgccga ttacctcgag gcccggccgc aaccataagt
     1681 ggaacgcgtt gccgaggaca atctgcgcgc cgcttgcggc cacttcctcc ggcgacagcg
     1741 cctttaccgt tccataggtt ccgaccggca tgaaggccgg cgtgtcgacg cggccgtggg
     1801 cgagctcgag aacgccgcgc cgggcggcgc cgtcgcgccg ggtgagggaa aacttcaaga
     1861 cctgccttcg atcagcatcg cgtcgccgta ggagaagaag cggtagcgac gggcgatcgc
     1921 atgggcatag gcgcgccgga tgttgtcgag gccggcaaaa gccgcgacca gcatgaggag
     1981 cgtcgagcgc ggcagatgaa agttggtgag caggcgcttc acgacccgga actcgaagcc
     2041 gggatagatg aaaagctcgg tctcgccttc gagcctgccg gtgagcgccg ccgtttccat
     2101 cgcgcgcagc gaagtcgtgc cgacggcgag aacgcgcttt ccgggcagag attcgaacgt
     2161 tccctcgggg
//
LOCUS       DASUNT010000581         2313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_601849, whole genome shotgun sequence.
ACCESSION   DASUNT010000581 DASUNT010000000
VERSION     DASUNT010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601849"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VFX94_11770"
     CDS             complement(<1..725)
                     /locus_tag="VFX94_11770"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HEX5768904.1"
                     /translation="MRDADVPGFLLRVQPSGVKSFIVSWGRGRRKTIGRVGKHTVEQM
                     RLKARRILLDAEEHGEPSVATRRSGTLGDFFHEHYKPHVEANHKDHVGNLKAIETEFA
                     YLFNKPLTAISAFDLEKYRLAKLKAGVKPATVNRHFDRIRSVFARALEWKAVETHPLR
                     GLKRSKVDRTGVVRFLSNAEEAALRNALDARDKRMRLERENANAWRAERNYPPFPKIP
                     DDGFGDVLTPMVLLSMNTGMRRG"
     gene            991..1224
                     /locus_tag="VFX94_11775"
     CDS             991..1224
                     /locus_tag="VFX94_11775"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HEX5768905.1"
                     /translation="MGRLVVVIVLVVLAAWLVRRAWRASKSSVSSRSAGPAQQDLVSC
                     ARCGMHLPRSEAREAGGQLYCGEEHARLGAGGP"
     gene            1236..>2313
                     /locus_tag="VFX94_11780"
     CDS             1236..>2313
                     /locus_tag="VFX94_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003273172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX5768906.1"
                     /translation="MRGASVTLPEVFLQPEERIPESFWTSLRYFGYYRIALAAVFLGS
                     ALIYEDAMNLGARLEVFRYLAAGYLLCALLVHVLLRDVRDKFELQLTLQVCLDIVAIT
                     LLMYASGGIRSGLGVMLLISLTGAAIVAPRRLTYLYAALAAIALLLEQSYWVLEHDAP
                     TASFVQPGLLAIGYFAIAGVTSGLAARVAANERLARQRGRELATQTRVNQLVIEDMND
                     GVLVLDRAGRVVQHNPQAQQLLGAPSLLGANIASLLPGFAGSAEIEVDGRDIRLRPLE
                     RGAEEFSLLLVEDTTRAREQAQQLKLAALGRLTANIAHEIRNPLAAISHATELLSEEK
                     RAEDRERLTRIIRDNAGRLERLVSD"
BASE COUNT          361 a          752 c          789 g          411 t
ORIGIN      
        1 tcgccccggc gcatgccggt gttcatggac agcaagacca tcggcgtaag cacatccccg
       61 aaaccgtcgt ccgggatctt cgggaaaggc gggtagttgc gctcggcgcg ccaggcgttg
      121 gcgttctccc gctcaaggcg catccgcttg tcgcgtgcgt ccagcgcatt gcgaagcgct
      181 gcctcctcgg cgttgctgag gaagcgcacc acgcccgtgc ggtcgacctt ggagcgcttc
      241 agccctcgga ggggatgcgt ctcgacggcc ttccattcga gggcgcgcgc gaacacggag
      301 cggatgcggt cgaagtgccg gttcaccgtc gccggcttca cgccggcctt gagcttggcg
      361 agccggtact tctccaggtc gaacgcggaa atcgccgtca gcggcttgtt gaacaggtac
      421 gcgaactcgg tctcgatcgc cttcaggttg ccgacgtggt ctttgtggtt cgcctcgacg
      481 tgcggcttat aatgctcgtg gaagaaatcg cccagagtcc ccgaacgcct cgttgcgaca
      541 ctcggctcgc cgtgctcctc ggcatcgagc agaatccggc gggccttcag ccgcatttgc
      601 tcgacggtgt gcttgccgac gcggccgatg gtcttgcggc gaccgcgccc ccatgaaacg
      661 ataaagctct tgacgccgga cggctgcacg cgcagcagaa agcctggaac gtccgcgtcg
      721 cgcacctcga aaggggccga ccggggcttc gcccgagcga tgacggaagg ggagagcttt
      781 acccgcatgc tgcctccgta agtgcttggt aagtgcaaaa ggaagtatag agcggggcta
      841 aagcggtacg caatgaaact ataagctgtt gaagtctaag gtaaatcaat gattggtgag
      901 ggtgtgggtt ttgcgttcga gacgcaggtg tctctggttc aaatccagtc gccccgacca
      961 tcgttttcgt tcccgttaga taatccggcg atgggccgcc tcgtcgtcgt catcgttctc
     1021 gtggtgctcg ccgcttggct ggtgcgccgc gcctggcggg cttccaagag cagcgtttcg
     1081 agccgcagtg ccgggcccgc gcagcaggac ctcgtgagtt gcgcgcgctg cggcatgcat
     1141 ctgccgcgca gcgaggcgcg cgaagcgggc gggcagctct actgcgggga ggagcatgct
     1201 cgcctcggcg cgggcggtcc ctgatggaag cgtcgatgcg cggtgcctcg gtcaccctgc
     1261 cggaagtctt cctgcagccg gaagagcgca ttccggagtc gttctggacg tcgctgcgct
     1321 acttcggcta ctaccgcatc gcgcttgcgg ccgtgttcct cggcagcgcg ctgatctacg
     1381 aggacgcgat gaacctcggc gcgcggctgg aggtgtttcg ctacctcgcc gccggctacc
     1441 ttctctgcgc gctcctggtg catgtgctgc tgcgcgacgt acgggacaag ttcgagctgc
     1501 agctcacctt gcaggtatgc ctcgatatcg tggccatcac gctgctcatg tacgcaagcg
     1561 gcgggatacg aagcggactg ggcgtgatgc tgcttatttc gcttaccggc gcggcgatcg
     1621 tcgcgccgcg gcggctgacc tacctctatg ccgcgctcgc cgccatcgcg ctgctcctcg
     1681 agcagagcta ctgggtgctc gagcacgacg cgccgaccgc gagcttcgtg cagcccgggc
     1741 tgctcgccat cggctacttt gccatcgccg gcgtgacgag cgggctcgcg gcgcgcgtcg
     1801 cggcaaacga gcgcctcgcg cgccagcgcg ggcgcgagct cgcgacgcag acgcgcgtca
     1861 accagctggt gatcgaagac atgaacgacg gcgtgctggt gctcgaccgc gccggccgcg
     1921 tggtgcagca taatccgcag gcccagcagc ttctcggcgc gcccagcctg ctcggcgcga
     1981 acatcgccag cctgctgccg ggctttgcgg gcagcgcgga gatcgaggtc gacggacgcg
     2041 acatccggct gcgcccgctc gagcgcgggg ccgaagagtt ttccctgttg ctggtggagg
     2101 acacgacgcg cgcgcgggag caggcgcagc agctcaagct cgcggcgcta gggcggctca
     2161 ccgccaacat cgcgcacgag atccgcaatc cgctcgccgc catcagccac gcgaccgagc
     2221 ttctgagcga ggagaagcgc gccgaggatc gcgagcgcct gacgcgcatc attcgcgaca
     2281 acgcggggcg cctggagcgg ctggtgtcgg acg
//
LOCUS       DASUNT010000582         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_341040, whole genome shotgun sequence.
ACCESSION   DASUNT010000582 DASUNT010000000
VERSION     DASUNT010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341040"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFX94_11785"
     CDS             complement(<1..401)
                     /locus_tag="VFX94_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5768907.1"
                     /translation="MHGLMMDQPLLIRDLIRHAERHHGSTEIVSKTVEGGIHRYTYRE
                     AHARARRLANALKRLGVAMHDRVATLAWNGFRHFEVYYAVAGSGAVIHTINPRLFPEQ
                     IAYIANHAEDKIVLYDATFAPLIEKLKPQLKT"
     gene            437..1363
                     /gene="bioB"
                     /locus_tag="VFX94_11790"
     CDS             437..1363
                     /gene="bioB"
                     /locus_tag="VFX94_11790"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX5768908.1"
                     /translation="MTVKDVLGLFGLPFTELVYRAAAVHRGHFDPSQIQLSTLLSVKT
                     GGCPEDCAYCPQSRRYATGVEDEEILPLEEVIACAKTAKANGASRFCMGAAWRGPKDR
                     DLEKVAVFIREVKALGLETCVTLGMLKPGQAERLRDAGLDYYNHNLDTSPQFYGEIIR
                     TRTYRDRLETLERVRAAGIRVCCGGIVGMGESREDRAALIAQLASLDPQPESVPINSL
                     IQVKGTPLHGVVPLDPLEFVRTIAVARICMPKAWVRLSAGREAMPEALQALCFLAGAN
                     SVFYGDKLLTTPNPETLTDRALFERLGLRPTA"
     gene            1383..>2099
                     /locus_tag="VFX94_11795"
     CDS             1383..>2099
                     /locus_tag="VFX94_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5768909.1"
                     /translation="MSDVAHYAVRDGIAVITMNNPPVNGLGNALRAALMAHLKKAEAD
                     AAVKGVVIIGSAKAFSGGADIREFGQPREKPDLFEVNDQQDAMSKPLVAAIGGFALGG
                     GLELALGCHYRVAVPRSQLGLPEVKLGILPGSGGTQRLPRVIPMVEAVRMMTTGATIP
                     AEKAKELGLVDEIVTGDLLEGAIEFLQKPRPIRRIRDMRARIDGDAQEFFANVREQVA
                     KDSRGYPAPLEIVACAEAAAT"
BASE COUNT          343 a          706 c          713 g          337 t
ORIGIN      
        1 gtcttgagct gcggcttgag cttctcgatg agcggcgcga aagtcgcgtc gtagaggacg
       61 atcttgtcct cggcgtggtt cgcgatgtag gcgatctgct cggggaataa acgcgggttg
      121 atggtgtgga tcacggcgcc cgagcccgcg accgcgtagt agacctcgaa atggcgaaag
      181 ccgttccagg cgagcgtcgc cacccggtcg tgcatcgcga cgccgaggcg cttgagcgcg
      241 ttggcgagcc ggcgcgcgcg ggcgtgcgcc tcccggtagg tgtagcggtg gatgccgccc
      301 tccaccgtct tcgacacgat ctcggtcgag ccgtggtggc gctcggcgtg gcggatcagg
      361 tcgcggatga gaagcggctg atccatcatc agcccatgca tgactgcctc ctcgcgaagt
      421 agtttatcct agcgccatga ctgtcaagga cgtgctcggg ctgttcggcc tgcccttcac
      481 cgagctcgtc taccgggccg ccgccgtgca ccgcggccat ttcgatccgt cgcagatcca
      541 gctctccacc ctgctctcgg tgaagaccgg cggctgcccg gaggactgcg cctactgccc
      601 gcagtcgcgc cgctacgcca ccggggtaga ggatgaagag atcctgccac tggaggaggt
      661 gattgcctgc gcgaaaaccg cgaaggcaaa tggtgcaagc cgcttctgca tgggcgcggc
      721 ctggcgcggg ccgaaggatc gcgacctcga gaaggttgcc gtcttcatca gggaagtgaa
      781 ggcattgggc ctcgaaacct gcgtgacgct cggcatgctc aagcccggcc aggccgagcg
      841 gctgcgcgac gccgggctcg actactacaa ccacaacctc gacacctcgc cgcagttcta
      901 cggcgagatc atccgcacgc gcacctaccg cgatcgcctg gagacgctcg agcgcgtgcg
      961 cgccgccggc atccgcgtgt gctgcggcgg catcgtcggc atgggcgagt cgcgcgagga
     1021 cagggcggcg ctcatcgcgc agctcgcgag cctcgatccc cagcccgaga gcgtcccgat
     1081 caattcgttg attcaagtca aaggcacgcc gctccacggt gtcgttcctc ttgatcccct
     1141 cgagttcgta cgcaccatcg cggtggcgcg gatctgcatg ccgaaggcgt gggtgcgcct
     1201 gtccgcgggg cgcgaagcga tgcccgaagc gttgcaggcg ctgtgcttcc tcgccggcgc
     1261 gaactcggtg ttctacggcg acaagctcct caccaccccg aacccggaaa cgctcacgga
     1321 ccgggcgctc ttcgagcgcc tgggcctgag acccaccgct taactccagg agtgcctctc
     1381 gcatgagcga cgtcgctcac tacgctgtcc gcgacgggat cgcggtcatc accatgaaca
     1441 atccgccggt gaacgggctg ggcaacgcgc tgcgcgccgc gttgatggcg cacctcaaga
     1501 aagccgaggc cgatgcggcg gtgaagggcg tggtgatcat cggctcggcg aaagcgttct
     1561 ccggcggcgc cgacatccgc gagttcggac aaccgcgcga gaagcccgac ctcttcgagg
     1621 tgaacgacca gcaggacgcg atgtcgaagc cgctcgtcgc ggcgatcggc ggcttcgcgc
     1681 tcggcggcgg cctcgagctc gcgctcggct gccactaccg cgtcgctgtt ccgcgctcgc
     1741 agctcgggct gcccgaagtc aagctcggca tccttcccgg ctcgggcggc acccagcgcc
     1801 tgccgcgcgt catcccgatg gtcgaagcgg tgcgcatgat gaccaccggc gccacgatcc
     1861 ccgccgagaa ggcgaaggag ctcggcctgg tggacgagat cgtgaccggg gatttgctgg
     1921 aaggagccat cgagttcctg cagaagcccc ggccgatccg ccgtatccgg gacatgaggg
     1981 caaggatcga cggcgacgcg caggagtttt tcgccaacgt ccgcgagcag gtggcgaagg
     2041 actcgcgcgg ctaccccgcg ccgctcgaga tcgtggcgtg cgccgaagcc gccgcgacg
//
LOCUS       DASUNT010000583         7020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1304778, whole genome shotgun sequence.
ACCESSION   DASUNT010000583 DASUNT010000000
VERSION     DASUNT010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7020
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304778"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VFX94_11800"
     CDS             complement(<1..634)
                     /locus_tag="VFX94_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5768910.1"
                     /translation="MSRWGKLGLVLLLIGLALAPLGLKNYGIYLLTLWCIYVIAGMGL
                     NLTVGYAGQMSIGHAAFLGIGAYTAAILMKMGFSFWFVLPLAALHCFLVGLVIGFPAL
                     RVQHHYLAFATLGFNILVYLFVRNEEWLTGGTFGISGLQRPGLLGVSLDAAASYYYFT
                     FASTILLALVLWWVIRSPWGRAFAALRDNPIRAESLGVNIMAYTLLAFAIG"
     gene            complement(631..1506)
                     /locus_tag="VFX94_11805"
     CDS             complement(631..1506)
                     /locus_tag="VFX94_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5768911.1"
                     /translation="MSDFVQLLISGTATGAIYALAALGFTLLWQASGTINFAQGEFVM
                     VPAFLTLSGIVLGAPLWAAFLIACAASMLVLGLGFKRVIVDPLIRHGVIPLVIATLGL
                     SIGIKNFVKSSYSAEAHPFPNVFPDTLFSLGGVTVSVYDIGTLALAGAIVLGLQVFLN
                     KTVTGRAMQAVAQNTEAATVLGINVRRMILYVFLINAALAAVAALLVTPVYLAKFDMG
                     DVIGLKAFYAAIIGGFNQTRGALLGGVLVGILENLVGAYVSPAYKEGVALMLFLVVIL
                     FRPEGLLGKAEERKV"
     gene            complement(1503..2345)
                     /locus_tag="VFX94_11810"
     CDS             complement(1503..2345)
                     /locus_tag="VFX94_11810"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX5768912.1"
                     /translation="MLIGLIGAGIQRSLSPALQEEEARHHGLRLHYQLIDLEEAGVGP
                     EVLPELIRAARVVGFAGLNVTFPCKQSVIPLLDELSEEARSIGAVNTVVREGQRLVGY
                     NTDGSGWSWGFRRALPGADLSRVVLLGAGGAGSACADAVLRLGAQQLVVVDQDASRAA
                     ALAARLNGQFSNRTSAAHDVATALQGATGLIHATPTGMLKIPGMPLPEALLRPSLWVS
                     EVVYVPLETQLLQAARRAGCATVDGGHMNVGQATRGFKLFTGLEADAARMEAHFRRLV
                     SGGA"
     gene            complement(2351..2947)
                     /locus_tag="VFX94_11815"
     CDS             complement(2351..2947)
                     /locus_tag="VFX94_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768913.1"
                     /translation="MLGAAAVAMWWDVAPEMKAEFEDWHSHEHMPERLAIPGFLRGSR
                     WIAASGEPSYFVLYEAASLATLTAGPYMERLNNPTPWSRKMMPHHRNMVRSLCEVRAS
                     FGRGLAQALATLRLDVPADALAPLPERRGLTAAHLLESRPMSGMPQTTEQRIRGGDAE
                     AKWIALIAGYDVDAVAAACREIAPRATPGVYRLSFCLT"
     gene            complement(2948..3709)
                     /gene="aroD"
                     /locus_tag="VFX94_11820"
     CDS             complement(2948..3709)
                     /gene="aroD"
                     /locus_tag="VFX94_11820"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEX5768914.1"
                     /translation="MKSIRIRGKPAWYPLICAPLVGADREALLAEGATALAARADIVE
                     WRVDHFADIGNLERVMETGRALRRALPGMPLLFTRRSVAEGGQPVPISEGEIVEVYSA
                     VCAEGFADLVDYEMSSPAQNMKAVREVSRRHDVGLVYSYHDFERTPPLDALAAQFRRA
                     QELGGDVAKVSVMARGPEDALALLAATLKASQTLELPLIGVSMGPHGALSRMVGFAFG
                     SALTFGVAAGSSAPGQMPIGELRAAIEIARKALGG"
     gene            complement(3706..4746)
                     /locus_tag="VFX94_11825"
     CDS             complement(3706..4746)
                     /locus_tag="VFX94_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5768915.1"
                     /translation="MKTRIAVAGAGYIGRAHIAALRTSPTCTLAAIVDPSPGAAKAAA
                     EAGVALFSSIEELLRKDRPDGVVLATPNQLHVQQALQCVEARLPTFVEKPIAQKVSEG
                     EKLVRRVEKDGARVLIGHHRAHSPIMAKAKEVLDSGVLGRLVAVMGSATFFKPDHYFV
                     DGPWRREPGAGPILLNMIHEVHNLRMLCGEIAAVQAIASHAVRAFPVEDTVAINLQFA
                     NGALGSFMLSDTAACPRSWEQTSQENKAYPSYDDEDCYVIAGTNGSLSVPTLRLKTYP
                     RPEDRSWWKPFDVGTVPMRRADPIVRQMEHFGAVVRGEAEPLVSARDGLANLRVTEAI
                     TEAAKSGGIVRL"
     gene            complement(4743..5708)
                     /locus_tag="VFX94_11830"
     CDS             complement(4743..5708)
                     /locus_tag="VFX94_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX5768916.1"
                     /translation="MAALLAVGTTQAQERSLKFATQNPKGHPITMGMEKFAELAAAKS
                     GGKMKVQLFPGGTLGSDQHNVSALQGGTLEIVSLNSGILASQAKEFGVYDFPFMFASP
                     KEADAVVDGAFGKMMHDKLESKGIVGLAYWELGFRNITNSRRAIRKVEDIAGLKLRVI
                     PNAINVDWVKALGANPTPMPFPEVYAALDQKAIDGQENPVTVINANKFYEVQKHLVIT
                     NHQYNPQSVIFSKKVWDSLSADEQKILRDAAMESAKFQRQASREQAGVALENLKKNGM
                     QVTELAPADLAKFQAAMKPVVAKHSEAVGPDVVKALEAELAKLRK"
     gene            complement(5755..>7020)
                     /locus_tag="VFX94_11835"
     CDS             complement(5755..>7020)
                     /locus_tag="VFX94_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX5768917.1"
                     /translation="FLGSLIGAMALGIPIAFALLACGVALMVHLNQFDAQILAQNLIN
                     GADSFPLLAVPFFILAGEIMNAGGLSKRIVDLALALVGHIRGGLGYVTIMAAILLSAL
                     SGSAVADAAALTAFLLPMMRKAGHDAARSGGLIAAASVVGPVIPPSIAFVIFGVAANV
                     SISKLFLAGIFPGILIGAALWITWWWLGRREIIKPPARKRLPEVLGALRESVWALMLP
                     LIILVGLRFGVFTPTEAAVVAAVYALFVAMAVYGELPLRKLYRVFVNAAKLSAVIMFL
                     VAAAMVSAWLITIAQIPAQLVALLQPFLDNKTLLMIAIMVLVLAVGTAMDMTPTILIL
                     TPVLMPVIKAAGIDPVYFGVLFIINNSIGLITPPVGTVLNTVAGVGRMRLDNVMRGSL
                     PFLLAEMAVLLLMVLFPPLVIVPARWLSG"
BASE COUNT         1262 a         2321 c         2325 g         1112 t
ORIGIN      
        1 cgccgatcgc gaaagcgagc agcgtgtacg ccatgatgtt gacgccgagg ctctcggcgc
       61 ggatggggtt gtcgcgcagc gcggcgaagg cgcggcccca gggtgagcga atcacccacc
      121 acagcacgag ggcaagaagg atggtggacg cgaacgtgaa gtagtaatag gacgccgcgg
      181 cgtcgagcga aacgccgagt aacccaggcc gctgtaggcc cgagatgccg aacgtgcctc
      241 cggtcagcca ctcttcgttg cggacgaaga ggtacacgag gatgttgaat cccagcgtgg
      301 cgaaagcgag gtagtggtgc tgaacgcgca gcgcgggaaa gccgatcacc aggcccacca
      361 ggaagcaatg cagcgccgcg agcggcagca cgaaccagaa ggagaagccc atcttcatca
      421 gaatggcggc ggtgtacgcg ccaataccga gaaacgcggc gtggccgatc gacatctgcc
      481 cggcgtagcc caccgtgaga ttgagcccca tgccggcgat gacatagatg caccacagcg
      541 tgagcaggta gatgccgtag tttttcagtc ccagcggagc gagtgccagt ccgatcaaga
      601 gcaacaccag cccgagtttc ccccaccggc tcatactttg cgctcctcgg ccttgccgag
      661 cagcccttcg ggccggaaca ggatcacgac gaggaagagc atcaacgcga cgccttcctt
      721 ataggcgggc gagacatatg cacccacgag gttctcgagg atgccgacca gcacgccgcc
      781 aagcagcgcg ccgcgcgtct ggttgaagcc gccgatgatc gcggcgtaga acgccttcag
      841 gccgatgacg tcgcccatgt cgaacttggc gagatagacc ggtgtcacga gcagcgcggc
      901 gacggcggcg agggccgcgt tgatcaggaa cacatagagg atcatgcgcc tcacgttgat
      961 cccgagcact gtcgcggctt ccgtgttttg cgccaccgcc tgcatcgcgc gccccgtcac
     1021 ggtcttgttg aggaagacct gcagcccgag cacgatggcg cccgcgagcg caagcgtgcc
     1081 gatgtcgtag accgagacgg tcacgccccc gagcgaaaac agcgtgtccg ggaatacgtt
     1141 cgggaaagga tgggcttccg cgctgtagct cgacttgacg aaattcttta tgccgatcga
     1201 aagcccgagg gtggcgatga cgaggggaat cacgccgtgc cggatgagcg ggtcgacgat
     1261 caccctcttg aagcccaggc cgagcaccag catggacgcg gcgcaagcga tcaggaacgc
     1321 ggcccacagc ggcgccccga gcacaatgcc ggagagcgtg aggaaggcgg gcaccatcac
     1381 gaactcgccc tgcgcgaaat tgatcgtgcc cgaggcctgc cacagaagcg tgaagccgag
     1441 cgcggcgagc gcatagatcg caccggtcgc cgtgccggaa atcagcaact gcacgaaatc
     1501 gctcaagccc ctcccgagac cagcctgcgg aagtgtgctt ccattcgggc cgcatcggct
     1561 tcaaggccgg taaacagctt gaagccgcga gtcgcctggc cgacattcat gtggccgccg
     1621 tcgaccgtgg cgcagccggc acgccgggcc gcctgcagga gctgcgtctc gagcggcaca
     1681 tacaccacct cggaaaccca aagcgagggg cgcagcagcg cttcgggcag cggcatgccg
     1741 ggtatcttca gcatgccggt cggcgtggcg tggatcagtc cggttgcgcc ttgcagcgcc
     1801 gtcgcaacat cgtgcgccgc acttgtgcgg tttgaaaatt gaccgttcag tcgggccgca
     1861 agcgctgccg cccgggacgc gtcctggtcc acgacgacga gctgctgcgc gccaaggcgc
     1921 agcacggcgt cggcgcaggc cgatcccgcg ccgcctgcgc cgagcagcac tacgcgagag
     1981 agatcggcac ccggcagcgc gcgcctgaag ccccagctcc aacccgagcc gtccgtgttg
     2041 tagccgacga gccgctggcc ctcccgcacg acggtgttga ccgcgccgat cgacctcgct
     2101 tcctccgaca gctcgtcgag cagcgggatc accgactgct tgcacggaaa agtgacgttc
     2161 aagccggcaa agccgacgac gcgagctgcg cggataagct cgggcaagac ttcaggcccc
     2221 acgcctgctt cctccaggtc gattagctga tagtgcagcc gcaggccatg atggcgcgcc
     2281 tcttcttcct gcagcgccgg cgacagtgag cgctggatgc ctgcgccgat gaggccgatg
     2341 agcattttcc tcatgtcagg cagaaggaca gccggtaaac gcccggcgtg gcgcgtggcg
     2401 cgatttctcg gcacgcggcc gctaccgcat cgacatcgta gccggcgatg agcgcaatcc
     2461 acttcgcttc ggcgtccccg ccgcggattc tctgctcggt ggtctgcggc ataccgctca
     2521 tcggccggga ttccaggagg tgcgctgccg tgagacctct acgctcgggt agcggcgcta
     2581 gtgcgtcggc tggaacatcg agccgcagcg tggccaaggc ctgtgcaagg ccgcggccaa
     2641 agctcgcccg tacctcgcac aagctgcgca ccatgttgcg gtgatgcggc atcatcttgc
     2701 gcgaccacgg cgtcggattg ttcaggcgtt ccatataagg cccggcggtc agtgtggcaa
     2761 ggctcgccgc ctcgtacagc acgaaatagg acggctcgcc ggaagccgcg atccaccgac
     2821 tgccgcgcag gaaacccggt atggccaggc gctcgggcat gtgctcgtgg gagtgccaat
     2881 cctcgaactc ggccttcatc tccggtgcga cgtcccacca catggccacg gcggcggcgc
     2941 cgagcactca cccgcctaat gctttgcgcg cgatctcgat cgcggcccgt agctcgccga
     3001 tcggcatctg gcccggcgcc gagctgcccg cggcaacccc gaaggtgagc gccgagccga
     3061 aagcgaagcc caccatgcgg ctgagggcgc cgtggggccc catggaaacg ccgatgagcg
     3121 gcagctcgag cgtctgcgac gccttcagcg tcgctgcaag aagtgcgagc gcgtcttcgg
     3181 ggccccgcgc catgaccgac accttcgcta cgtcgccgcc cagctcctgc gcgcggcgga
     3241 actgcgcggc tagcgcatcg agaggcggtg tgcgctcgaa atcgtggtac gagtacacga
     3301 ggccgacatc gtggcggcga gagacctcgc gcaccgcctt catgttctgc gcggggctgc
     3361 tcatctcgta gtcgaccagg tcggcaaagc cctcggcgca gaccgctgaa tagacctcga
     3421 cgatttcgcc ctcggagatt ggcaccggct gtccgccttc ggccacggag cggcgcgtaa
     3481 agaggagcgg catgccgggc agcgcgcgcc gcagggcacg gccggtctcc atgactcttt
     3541 ccagatttcc aatgtccgcg aaatggtcga cccgccattc gacgatgtcg gcgcgcgccg
     3601 cgagagccgt cgcgccttcc gcgagcagcg cttcccggtc agcgccgacg agcggcgcgc
     3661 agatcagggg gtaccaggcg ggcttgccgc ggatgcggat cgacttcaca gccgcacgat
     3721 gccgccgctc ttggccgcct cggtgatggc ctcggtaacg cgcaggttgg caaggccgtc
     3781 gcgcgcgctc accagcggct cggcctcccc ccgcaccacc gcgccgaaat gctccatctg
     3841 ccgcacgatc gggtccgcgc gcctcatcgg caccgtgccg acgtcgaacg gcttccacca
     3901 cgaccggtcc tcgggacggg gataggtctt caaccgcagg gtcggcaccg aaagactgcc
     3961 gttggtgccg gcgatcacgt agcagtcttc gtcgtcgtag ctcggataag ccttgttctc
     4021 ctgcgaagtc tgctcccagc tgcgcgggca ggcggcggta tccgagagca tgaagctgcc
     4081 gagcgctccg ttcgcgaatt ggagattgat tgcaacggtg tcctcaaccg ggaaggcgcg
     4141 caccgcgtgc gaggcgattg cttgcacggc agcgatttcc ccgcacagca tgcgcaggtt
     4201 gtgcacctcg tggatcatgt tgagcaggat aggccccgcg cccggctcgc ggcgccacgg
     4261 cccgtctacg aagtaatggt cgggcttgaa gaaggtcgca ctgcccatca ctgccaccag
     4321 ccgcccgagc actcccgaat cgagcacctc cttcgccttg gccatgatcg ggctgtgcgc
     4381 gcggtgatgc ccgatgagca ctctcgcccc gtctttctcc acccgtctta cgagcttttc
     4441 gccttcgctg accttctgcg cgatcggctt ttccacgaag gtgggcaaac gcgcctcgac
     4501 gcattgcagc gcctgctgca cgtggagctg gttcggcgtg gcgaggacta cgccgtcggg
     4561 gcggtccttc cgcagaagct cctcaatcga ggaaaacagc gctaccccgg cttccgcggc
     4621 ggctttcgcg gctccgggcg acgggtcgac gatggcggcg agcgtgcacg tcgggctcgt
     4681 ccgcagcgcg gcgatgtggg cgcggccgat gtagccggcg cccgccaccg cgatgcgggt
     4741 cttcatttac gcagcttggc gagctcggct tcgagcgcct tgacgacgtc ggggcccacc
     4801 gcctccgagt gcttcgcgac caccggcttc atcgccgcct ggaatttcgc gagatccgcc
     4861 ggggccaatt cggtgacctg catgccgttc ttcttcaggt tctcgagcgc cacgccggcc
     4921 tgctcgcgcg aggcctggcg ctggaatttc gccgactcca ttgcggcgtc gcgcaggatc
     4981 ttctgctcat ccgcgctcag cgagtcccag actttcttgc tgaagatcac cgactgcggg
     5041 ttgtactgat ggtttgtgat caccaggtgc ttctgcacct cgtagaactt gttcgcgttg
     5101 atcacggtca ccgggttctc ctgcccgtcg atcgccttct ggtcgagcgc cgcgtacacc
     5161 tccgggaacg gcatcggcgt cggattggcg ccgagcgcct tcacccagtc gacgttgatc
     5221 gcgttcggga tgacgcgcag cttgagcccg gcgatgtctt ccactttgcg gatcgcgcgc
     5281 cggctgttgg tgatgttgcg gaagccgagc tcccagtagg cgaggccgac gatgcctttg
     5341 gactcgagct tgtcgtgcat catcttgccg aaggcgccgt cgaccaccgc gtcagcctcc
     5401 ttcgggctgg cgaacatgaa agggaagtcg tagacgccga actccttcgc ctgcgaggcg
     5461 aggatgccgg agttgagcga cacgatttcc agcgtgccgc cctgcagcgc cgagacgttg
     5521 tgctggtcgc tgccgagcgt gccgccgggg aagagctgca ccttcatctt gccgccgctc
     5581 ttggccgcgg ccagctcggc gaacttttcc atgcccatgg tgatcgggtg gcccttgggg
     5641 ttctgggtcg cgaacttgag gctgcgctcc tgcgcctgcg tcgtgcctac ggcaagcagg
     5701 gccgccataa aagtgaatct gaatacaggt ttcatcaggg tctccttgga aaagttagcc
     5761 ggacagccag cgcgccggca cgatcaccag cggcgggaaa agtaccatca gaagcagcac
     5821 cgccatctcg gcgagcagga aaggcagcga gccgcgcatc acgttgtcga gcctcatgcg
     5881 gcccacgccg gccacggtgt tgagcaccgt gccgaccggt ggcgtgatga ggccgatcga
     5941 gttgttgatg atgaacagca cgccgaagta gaccggatcg atgcctgccg ccttgatcac
     6001 cggcatcagc accggcgtga ggatcaggat cgtcggggtc atgtccatcg ccgtgccgac
     6061 cgcgagcacg agcaccatga tggcgatcat cagcagcgtc ttgttgtcga gaaagggctg
     6121 cagcagcgcg acgagctgcg ccggtatctg cgcgatggta atgagccacg ccgagaccat
     6181 cgcggcggcg accaggaaca tgatcaccgc ggagagcttg gcggcgttca cgaagacgcg
     6241 gtagagcttg cgcagcggca gctcgccgta gaccgccatt gcgacgaaca gcgcatacac
     6301 cgcggccacc accgcggcct ccgtgggggt gaacacgccg aagcgcaggc cgacgaggat
     6361 gatgagcggc agcatcagcg cccagaccga ctcgcgcagc gcgcccagca cctccggcag
     6421 ccgcttgcgc gccggcggct tgatgatctc ccggcgtccg agccaccacc aggtaatcca
     6481 cagcgcggcg ccgatcagga tcccggggaa gattccggcg aggaacagtt tcgagatcga
     6541 cacgttcgcc gcgacgccga agatgacgaa ggcaatggac ggcggaatca ccggcccgac
     6601 cacgctcgct gcagcgatca ggccgcccga gcgggcggcg tcgtggccgg ccttgcgcat
     6661 catcggcagc aggaaggcgg tgagcgcggc ggcgtcggcc accgccgagc cggagagcgc
     6721 cgagagcagg atcgccgcca tgatagtgac gtagccaagc ccgccgcgga tgtggccgac
     6781 gagcgcgagc gcgagatcga cgattcgctt ggaaagcccg ccggcattca tgatctcgcc
     6841 ggcaaggatg aagaacggca cggcgagcag cgggaagctg tcggcgccgt tgatcaggtt
     6901 ttgcgcgagg atctgggcgt cgaactggtt gagatgcacc atcagcgcca cgccgcaggc
     6961 gagcagcgcg aacgcgatcg gaatgccgag cgccatggcg ccgatgagcg agccgaggaa
//
LOCUS       DASUNT010000584         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_777879, whole genome shotgun sequence.
ACCESSION   DASUNT010000584 DASUNT010000000
VERSION     DASUNT010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777879"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..372)
                     /gene="rpsQ"
                     /locus_tag="VFX94_11840"
     CDS             complement(118..372)
                     /gene="rpsQ"
                     /locus_tag="VFX94_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX5768918.1"
                     /translation="MSNQRTLMGRVTSNKMQKTIVVRVERRVRDVFMGKTITRSRKYH
                     AHVEEGQFGEGDLVEIAECRPISRTKSWRVTRLVEKSKAV"
     gene            complement(369..560)
                     /gene="rpmC"
                     /locus_tag="VFX94_11845"
     CDS             complement(369..560)
                     /gene="rpmC"
                     /locus_tag="VFX94_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX5768919.1"
                     /translation="MKANELRGKSGAELQKELEGLLRAQFGLRMQLATQQLSNTSQLK
                     KVKRDIARVRTIMKEKAAK"
     gene            complement(563..979)
                     /gene="rplP"
                     /locus_tag="VFX94_11850"
     CDS             complement(563..979)
                     /gene="rplP"
                     /locus_tag="VFX94_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX5768920.1"
                     /translation="MLQPARTKYRKQQKGRNTGNATRGAKVSFGEFGLKATTRGRLTA
                     RQLEAGRRAMSRHIKRGGRIWIRVFPDKPVSQKPAEVRMGNGKGNPEYFVCEIQPGKI
                     IFEMDGVTDALAKEAFALASAKLPFRTIVVQRMLGQ"
     gene            complement(983..1840)
                     /gene="rpsC"
                     /locus_tag="VFX94_11855"
     CDS             complement(983..1840)
                     /gene="rpsC"
                     /locus_tag="VFX94_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX5768921.1"
                     /translation="MGQKINPVGFRLAVNRNWSSRWYANSRSFAPMLAEDITVRDFLK
                     KKLAHASVGRVLIERPAKDARITIFSARPGVVIGKKGEEIESLKAELRRIMGVQQVHV
                     NIEEIRKPEIDAQLIADSIAQQLEKRIMFRRAMKRAMQNAMRLGAQGIKIMSAGRLNG
                     IEIARTEWYREGRVPLHTLRADIDYGLGEAKTTYGVIGIKVWVYKGEVMPKGDAAAAL
                     LAATTAPLPQPEAPVEPKRAKKPAPRAGARRGGPARPKKEGAPAAPAPKTVVKKPKKI
                     MKDEGEKKE"
     gene            complement(1840..>2021)
                     /gene="rplV"
                     /locus_tag="VFX94_11860"
     CDS             complement(1840..>2021)
                     /gene="rplV"
                     /locus_tag="VFX94_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX5768922.1"
                     /translation="LESAIANAEHNDGADIDALKVKSITVERGMFLKRFQARAKGRGN
                     RILKHTCHIYITVGD"
BASE COUNT          271 a          667 c          646 g          437 t
ORIGIN      
        1 cgcgcgccgg tgttatcggc gacgtccagc acggtctgca tctgaatcat ttcatgtctc
       61 cattccaact tgcactgccc ggcagtgcag ttttggaccc cgaaggggag cgcgattcta
      121 aacggccttg gacttctcca caaggcgcgt gacacgccag gatttggtcc gggagatcgg
      181 ccggcactcg gcgatctcga cgagatcgcc ctcgccgaac tggccttcct cgacgtgcgc
      241 gtggtatttc cgggagcgcg taatggtctt gcccatgaag acgtcgcgca cgcgccgctc
      301 gacgcgcacc acgatcgttt tctgcatctt gttgctcgtg acgcgtccca tcagggtccg
      361 ttgattgctc atttcgcggc tttctccttc atgatcgtgc ggactcgagc gatatcccgc
      421 ttcactttct tgagctggct ggtgttcgaa agctgctggg tcgcgagctg catgcgcagg
      481 ccgaactgcg cgcgcagcag gccttccagc tccttctgca gctctgcgcc gctcttgcct
      541 cggagttcgt ttgctttcat ggctactgtc cgagcatgcg ttgcacgacg atagtgcgga
      601 acggcaactt ggccgaggcg agtgcgaacg cctccttcgc cagcgcgtcg gtgacgccat
      661 ccatctcgaa gatgatcttg ccgggctgga tctcgcacac gaagtactcc gggttgccct
      721 tgccgttgcc catgcgcacc tcggcgggct tctgcgacac cggcttgtcg ggaaacacgc
      781 ggatccagat ccggccgccg cgcttgatgt ggcgcgacat ggcgcggcgc ccggcctcga
      841 gctggcgcgc cgtcagccgg ccgcgcgtcg tggccttgag gccgaactcg ccgaacgaca
      901 ctttggcgcc gcgcgtggcg ttgccggtgt tgcggccctt ctgctgcttg cggtacttgg
      961 ttctggcggg ttgcaacatc ggctattcct tcttttcgcc ttcgtctttc atgatcttct
     1021 tcggcttctt gaccaccgtc ttgggcgcgg gtgcggcggg agcaccctct ttcttcggcc
     1081 gcgccgggcc gccgcgcctt gcgcccgcgc gcggcgcggg cttcttcgcg cgcttgggct
     1141 cgaccggcgc ttcgggctgc ggcagcgggg cggtggtggc agcgagcagc gccgcggcgg
     1201 cgtcgccctt gggcatcacc tcgcccttgt agacccagac cttgatgccg atcacgccgt
     1261 aggtggtctt cgcctcgccg aggccgtaat cgatgtcggc acgcagcgtg tgcagcggca
     1321 cgcgaccctc gcggtaccac tcggtgcgcg cgatctcgat gccgttcagg cggccggcgc
     1381 tcatgatctt gatgccctgc gcgcccaggc gcatggcatt ctgcatggcg cgcttcatcg
     1441 cgcggcggaa catgatgcgc ttctcgagct gctgcgcgat ggaatcggcg atcagctgcg
     1501 cgtcgatctc gggcttgcgg atttcctcga tgtttacgtg cacctgctgt acgcccatga
     1561 tgcggcgcag ttccgccttg agcgactcga tctcctcgcc cttcttgccg atcaccacgc
     1621 ccgggcgcgc cgagaagatg gtgatgcggg cgtccttcgc cgggcgctcg atcagcaccc
     1681 ggccgaccga agcgtgggcg agcttcttct tcaggaagtc gcgcaccgtg atgtcctcgg
     1741 cgagcatcgg cgcgaacgag cggctgttcg cgtaccagcg cgaactccag ttgcggttga
     1801 ccgcgaggcg aaagcccacg gggttgatct tttggcccat cagtcaccca ccgtaatgta
     1861 gatatggcac gtgtgcttaa gtatgcggtt gccccgcccc ttggcgcggg cctggaagcg
     1921 cttgaggaac atgccgcgct cgaccgtgat cgactttacc ttgagcgcat cgatgtcggc
     1981 gccgtcgttg tgctcggcgt tggcgatcgc cgactcgagc a
//
LOCUS       DASUNT010000585         2824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1128621, whole genome shotgun sequence.
ACCESSION   DASUNT010000585 DASUNT010000000
VERSION     DASUNT010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128621"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VFX94_11865"
     CDS             complement(<1..651)
                     /locus_tag="VFX94_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5768923.1"
                     /translation="MRLKDKVAIVTGAGSGFGAGIARRFAEEGARVIVNDVDPKGDKI
                     AQEIGGRFVQADVTKGADWAKLVQAAGERLDIVVNNAGWTHRNKPYLEVSEAEFDRVY
                     AVNVKSVYLSALHAVPVFRKRGGGCFVNIASTAGVRPRPGLTVYNSSKGAVIIMSRSM
                     AAEFGPDRIRVNCVNPVFSPETSLSADFAGGALTEEAKKRFLSTIPLGRFSTPLDVA"
     gene            complement(648..1763)
                     /locus_tag="VFX94_11870"
     CDS             complement(648..1763)
                     /locus_tag="VFX94_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5768924.1"
                     /translation="MGSISIVQSLAGVDAGEWNSLAGRQPFVRHEFLCALIDTGCASP
                     ASGWAPSFVLARRAGALVGAMPLFAKSHSYGEYVFDWAWADAHERHGIDYYPKLLCAV
                     PFTPVRGARLLGTEKALLLDAAFELARGSSSLHVLFPREEDMPALAARGMLIRRTVQF
                     HWRNEGYVDFEDFLRRMTHARRKKIRQERRKVKEAGITFSWLEGPAISRRHWELFNRC
                     YRRTYAEHRSSPYLSLEFFLRIGAALPQNTALLVAERGARPIAASLFLKDEAALYGRY
                     WGALEHVPLLHFECCYYQAIEYAIARRLQAFEGGAQGEHKLWRGLLPVEAPSAHWLAH
                     PSFARAVEAFLEREGAGVARYVDELCEHSPFKKEEDK"
     gene            complement(1750..2319)
                     /locus_tag="VFX94_11875"
     CDS             complement(1750..2319)
                     /locus_tag="VFX94_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5768925.1"
                     /translation="MALSESLRPSLLFWYGQVLMLFKRHSSALEVFRSVTRESPRHQQ
                     AWASLAFLLAERDEHQAALDAYERAIALNPDDAASHFNVAFILQRIGRHEAALPRFER
                     AIEIDAKVDRAWYGLGLSLAHLGRLEEAAEKLQEAARLQPMNPYAGYQLAAVWHRLHR
                     RDKVRAQYERIKGFDPKVAERIRVEFGVH"
     gene            2394..2732
                     /locus_tag="VFX94_11880"
     CDS             2394..2732
                     /locus_tag="VFX94_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX5768926.1"
                     /translation="MKKIEAIVKPFKLDEVREALSEVGVSGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVVVANNQVESAIDAIVRSARTGKIGDGKIFVTSVEHVVRI
                     RTGEINEAAV"
BASE COUNT          497 a          942 c          937 g          448 t
ORIGIN      
        1 cgcgacatcg agcggcgtcg agaagcgccc gagcggtatg gtcgagagaa agcgcttctt
       61 ggcttcctcg gtgagcgcgc cgccggcgaa atcagccgag agtgaagtct cggggctgaa
      121 caccgggttc acgcagttga cacggatcct gtccggaccg aactccgcgg ccatcgacct
      181 cgacatgatg atcacggcgc ccttggagct gttgtagacc gtgagcccgg gccgcggccg
      241 cacgccggcg gtcgaggcga tgttcacgaa gcagccgccg ccccgcttgc ggaataccgg
      301 cacggcgtgc agcgcggaaa ggtagacact cttcacgttc accgcgtaca cccggtcgaa
      361 ctcggcctcg ctcacctcga ggtagggctt gttgcgatgc gtccagccgg cgttgttgac
      421 cacgatgtcg agccgctcgc ccgcggcctg cacgagcttc gcccagtcgg cgccctttgt
      481 gacgtcggcc tggacaaacc tgccgccgat ctcctgcgcg atcttgtcgc ctttgggatc
      541 gacgtcgttc acgattaccc gggcgccttc ctcggcgaag cgccgggcga tgccggcgcc
      601 aaagcccgag ccggcgccgg taacgatggc gaccttgtcc ttgagcctca tttgtcctcc
      661 tccttcttga acgggctatg ttcgcacagc tcgtccacgt agcgcgcgac gccggcaccc
      721 tcgcgctcga gaaaggcttc caccgcgcgc gcgaagctcg ggtgcgcgag ccagtgcgcc
      781 gacggcgcct cgaccggcag aagcccgcgc cagagcttgt gctcgccctg cgcgccgccc
      841 tcgaaagcct gcaggcgccg cgcgatggcg tactcgatcg cttggtagta gcagcattcg
      901 aaatgaagga gcggcacgtg ctccagcgca ccccaatagc ggccgtagag cgcggcttcg
      961 tccttgagga atagcgacgc ggcgatcggc cgcgcgccgc gctccgccac aagcagcgcg
     1021 gtgttctgcg gcagcgccgc gccgatacgg agaaagaact cgaggctgag atacggactc
     1081 gagcgatgct cggcgtacgt cctgcggtag cagcggttga agagctccca gtgccgccgg
     1141 ctaatcgccg gtccttcgag ccaggagaac gtgattccag cctccttcac cttgcggcgc
     1201 tcctggcgga tcttcttccg ccgcgcgtgc gtcatgcggc gcaggaagtc ctcgaagtcc
     1261 acatagccct cgttgcgcca gtgaaactgc acggtgcgcc tgatcagcat gccgcgtgcg
     1321 gccagcgccg gcatgtcttc ctcacgaggg aaaagcacat gcagcgacga gctaccgcgc
     1381 gcgagctcga aggcggcgtc gagaagcagc gccttttccg taccgaggag gcgagcgcct
     1441 cgcaccggcg tgaacggaac ggcgcagagc agcttggggt agtaatcgat tccgtgccgc
     1501 tcgtgcgcgt cggcccaggc ccagtcgaac acgtattcgc cgtaggaatg cgacttggcg
     1561 aagagcggca tggcgccgac gagcgccccg gcgcgccgcg ccagcacgaa gctcggcgcc
     1621 cacccgctcg ccggcgaggc gcatccggta tcgatcagcg cgcagaggaa ttcgtggcgc
     1681 acgaagggct gccgcccggc gagcgagttc cattcgccgg catccacgcc ggccagcgac
     1741 tggacgatgc taatggaccc caaattcgac gcggatcctc tcggccactt tgggatcgaa
     1801 gcctttgatc cgctcgtatt gcgcgcggac cttgtcgcgg cggtgtaagc ggtgccatac
     1861 ggcggcgagc tggtagccgg catacgggtt catcggctgc aggcgcgccg cctcctggag
     1921 cttctccgcc gcctcctcga gccgcccgag gtgcgcgagc gataggccga ggccgtacca
     1981 ggcgcggtcc acctttgcgt cgatttcgat cgcgcgctcg aaacggggca gggcagcctc
     2041 gtgccggccg atgcgctgca ggatgaacgc cacgttgaag tgcgacgctg catcgtcggg
     2101 attgagggcg atcgcccgct cgtacgcgtc gagcgccgcc tggtgctcgt cgcgctcggc
     2161 gagcaggaag gcgagcgagg cccaggcctg ctggtgccgg gggctctcgc gcgtgacgga
     2221 ccggaaaact tccagtgcgc tcgagtggcg cttgaacagc atcaggacct gcccgtacca
     2281 gaaaagcagc gacgggcgca gggactcgct caacgccatg ccggggattt tacgagcttt
     2341 gtcgctgtac aatgccccat ccaggagaag agggcgtacg gaggactgcg cggatgaaaa
     2401 agatcgaggc gatcgtcaag ccgttcaagc tcgatgaagt gcgcgaggcg ctttccgagg
     2461 tgggcgtcag cggcctcacg gtgaccgagg tgaaggggtt cggccggcag aagggccaca
     2521 ccgagctcta tcgcggcgcc gagtacgtcg tcgacttcct gcccaaggtc aagatcgaag
     2581 tcgtagtcgc gaacaaccag gtcgaaagcg cgatcgacgc catcgtgcgc tccgcccgca
     2641 ccggcaagat cggcgacggc aagatcttcg tcacctccgt cgagcacgtc gtccggatca
     2701 ggaccggcga aatcaacgaa gccgccgtct agaacaacgc tcgacgtcac cccgcggagg
     2761 aacgtcaccc ccgttttgac caacataggt tcccgcatgc gcgggaacga cgatcaccgc
     2821 gttc
//
LOCUS       DASUNT010000586         2550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1305049, whole genome shotgun sequence.
ACCESSION   DASUNT010000586 DASUNT010000000
VERSION     DASUNT010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305049"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1527)
                     /locus_tag="VFX94_11885"
     CDS             complement(34..1527)
                     /locus_tag="VFX94_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX5768927.1"
                     /translation="MLDPNRSDLVAGVAGAGTMGRGIAQVLAQCGARTLLYDAQPGAA
                     RKAKDSIAQALGKLVEKGRLGAGEADNAVGRIEIIDSVDAFGPSHVVVEAIVEDLNAK
                     QQLFSSLEKQVRSDCILASNTSSLSVTAMAAACQRPGRVAGYHFFNPVPVMKIVEVVD
                     GVLTEPWVTDALAALARRFGHTPVRCKDTPGFVVNHAGRAFVPEALRVLSEGIADFAT
                     IDRILVDAAGFRLGPFGLMDLVGLDVAHSVMKSMYRQYYEEPKYRPSFLAEPRVAAGL
                     LGRKTGRGWYEYGQEGPKAIAEASPPKIKPHAVWAVPELKELLFELGAKPELKPTPEA
                     VCFVAPLGTDATTSAFELGLDPQRTVAVDPLFGFSKRRTLMRTCITGKEILEAAHGLL
                     AADGVPVSVINDSPGFVAQRVVAHVVNVGCDIAQMRIASPEDLDRAVTLGLGYPRGPL
                     AMGDALGPKRVLAVLEAMHDFYQDPRYRPSPWLRRRAALGVSLLKEE"
     gene            1585..2529
                     /locus_tag="VFX94_11890"
     CDS             1585..2529
                     /locus_tag="VFX94_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5768928.1"
                     /translation="MRKLLALAGSLLLAAAAHAQQFPSKPVTLIVPWPAGGSTDIYFR
                     KLGEITARHLGQPLVIENRPGGSGMNGPATMAKTARPDGYTISQLAITAFRVPHMQKV
                     DWDPITDFTYIVGLAGYTFGIVVKADSPFKTFQDLLAYARANPSKLSYATPGTGTSLH
                     LAMEEIAAKAGVQFLHVPFKGYGDGAIALMGGHVMVQVDSTGWAKQVDSGAQRLLATL
                     GDKRTRWNAPTVKELGVDTVSNSPFGLVGPKGMPRDVVKVLHDAFKKSLDDPEYLKML
                     AQLDQPAWYMSSEDYARWAGDMLKAERATIERVGLLLK"
BASE COUNT          451 a          819 c          845 g          435 t
ORIGIN      
        1 taaatgtagg cgtctagcat ggggtggctt tctctattct tccttcaata aagagacccc
       61 gagcgcggcg cgtctgcgca gccacgggct ggggcgatag cgcgggtcct ggtagaaatc
      121 gtgcatcgct tcgagcaccg cgagcacgcg cttcggtccg agcgcgtcgc ccatcgcgag
      181 cgggccgcgc ggatagccga gcccgagggt aacggcgcgg tcgaggtctt ccgggctggc
      241 gatgcgcatc tgcgcaatgt cgcagccgac gttcacgacg tgcgcgacca cgcgctgcgc
      301 gacgaagccc ggcgagtcat tgatcaccga caccggcacg ccatcggcag caagaagccc
      361 gtgcgccgcc tccaggattt cttttcctgt aatgcaagtt ctcatcaagg tgcggcgctt
      421 ggagaagccg aacaggggat ctaccgctac cgtgcgctgc ggatcgagtc cgagctcgaa
      481 cgcgctcgtg gtcgcgtcgg taccgagcgg cgcgacgaag cagaccgcct cgggcgtcgg
      541 cttcaattcc ggcttcgccc cgagctcgaa cagcaattcc ttcagctccg gcaccgccca
      601 gacggcgtgc ggcttgattt tcggtgggga agcttcagca atcgcttttg gcccttcttg
      661 tccatactca taccatcctc gccccgtctt gcgtccgagc aatccggcag cgacgcgcgg
      721 ctcggcgagg aaggaggggc ggtacttcgg ctcctcgtag tactgccggt acatcgactt
      781 catgacggag tgcgcgacat caaggcctac cagatccatg agaccgaaag gtccgagccg
      841 gaatcccgca gcgtcgacga ggatgcgatc aatcgttgcg aaatcggcga tgccctcgga
      901 cagcacgcgc agcgcctcgg gcacgaatgc ccgcccggcg tggttgacga cgaatcccgg
      961 cgtgtccttg cagcgcaccg gcgtgtggcc gaagcggcgg gcaagcgcgg cgagcgcgtc
     1021 ggttacccac ggttcggtga ggactccgtc gaccacctcc acgatcttca ttaccggtac
     1081 cgggttgaaa aagtggtagc cggcgacgcg gccgggccgc tggcacgcag ctgccatcgc
     1141 ggtgacggag agcgaggagg tgttggacgc gagaatgcaa tcgcttctca cttgcttttc
     1201 gaggctggaa aagagctgct gcttcgcgtt caagtcttcg acaatggctt ccaccacgac
     1261 gtggctgggc ccgaacgcgt ccaccgagtc gatgatttct atccggccga cggcgttgtc
     1321 cgcctccccg gcgccgagcc gccctttctc gacgagcttg ccgagcgcct gcgcgatcga
     1381 gtctttcgcc ttgcgcgctg cgccgggctg ggcgtcatag agcagcgtcc gggcgccgca
     1441 ctgggcgaga acctgcgcga tgccgcggcc catggtaccc gcaccggcga cgccggcgac
     1501 gagatcgctg cggttgggat ctagcaagcc cttattctag cggccggagg tggcggctat
     1561 aatcgccgcg aggaggaggc gagcatgaga aagctactgg ccctggcggg aagcctgctg
     1621 ctggcggctg cggcgcacgc gcagcaattc ccgtcgaaac ccgtcacact gattgttccg
     1681 tggccggcgg gtggcagcac cgacatctac tttcggaagc tcggcgagat aactgcccgg
     1741 cacctgggtc agccgctcgt tatcgagaac cggccgggcg gcagcggaat gaatgggccg
     1801 gcgaccatgg cgaagaccgc gaggcccgac ggctacacca tctcgcagct cgccatcacg
     1861 gccttccgcg tgccgcacat gcagaaggtc gactgggacc cgatcacgga tttcacctac
     1921 atcgtcggcc ttgctgggta caccttcggc atcgtggtga aagccgactc gccgttcaag
     1981 accttccagg atctccttgc gtacgcgcgc gcgaacccca gcaagctttc gtacgccacg
     2041 ccgggaacgg gcacgtcgct ccatctcgcc atggaggaga tcgcggccaa ggccggcgtg
     2101 cagttcctcc acgtcccgtt caagggctac ggcgacggcg cgatcgcgct gatgggcggg
     2161 cacgtgatgg tgcaggtcga ttccaccggc tgggcgaaac aggtggactc gggcgcccag
     2221 cggctgctcg cgacgctcgg cgacaagcgc acgcgctgga atgcgccgac ggtcaaggag
     2281 ctcggcgtcg ataccgtatc gaactcgccg ttcggcctgg tgggcccgaa gggcatgccg
     2341 cgcgacgtag tcaaagtgct gcacgacgcg ttcaagaagt cgctggacga tcctgagtac
     2401 ctcaagatgc tggcgcagct cgatcagccc gcctggtaca tgtcgagcga ggattacgcg
     2461 cgctgggccg gcgatatgct gaaggccgag cgcgccacca tcgagcgggt ggggttactt
     2521 ctcaagtaga ggaggcgacc atgaagtatc
//
LOCUS       DASUNT010000587         3389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_428701, whole genome shotgun sequence.
ACCESSION   DASUNT010000587 DASUNT010000000
VERSION     DASUNT010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428701"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VFX94_11895"
     CDS             complement(<1..809)
                     /locus_tag="VFX94_11895"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX5768929.1"
                     /translation="MLPVSLGDAVELAAILILAVLLFLVLFEPPVAYRVRTPAAGVGS
                     REFVNYLSAIVNARVFAPGEVLVLNGGEQAFAAQLAAIRAARRSIHLEVYLFLRGRVA
                     DEMLAALEERARNGVRVRLVVDRYGSLLTPKRYFDGLKQAGGELHWYQPIAWYTLKRL
                     NNRTHRDLLIVDGEVAFIGGMGVADYWIGPPGRGQPWRDTMIRVCGELAEGLQTSFAE
                     NWLEASGEILAEEEFQAGAASPEAPVSGTEVALVANSSPSEGRATRARMLF"
     gene            862..1212
                     /locus_tag="VFX94_11900"
     CDS             862..1212
                     /locus_tag="VFX94_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768930.1"
                     /translation="MKTFAAYTAALAIGLATAVFILWLGRSTPVGTDISFAAASVVFA
                     LAIVARVLSAPLVHHWFGLALALSLPSCILGIVMFSLVARLGEYFWAWLWSALGALAA
                     SLIGAYFAARASRA"
     gene            complement(1219..1896)
                     /locus_tag="VFX94_11905"
     CDS             complement(1219..1896)
                     /locus_tag="VFX94_11905"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX5768931.1"
                     /translation="MINRSLAFAAMMLLVEPGAYAACSDAPSAGVDWSGCTKQQLVIR
                     GKNLQGARFERGTLLAVNFERSDLSGGSFAAAHVSRTSFRDAVLERANLSKLTAVRSS
                     FAGARLVGADLQKAELHRCVLTGAALRGVDMSKGDFGRSVFDRADLREANFKYASLAR
                     ASLREANLAGADFSQAHLFATNVEGTDLSAVKGLRQAQLDAACGDARTRLPPGLKPPS
                     GWPCAPD"
     gene            complement(1908..2414)
                     /locus_tag="VFX94_11910"
     CDS             complement(1908..2414)
                     /locus_tag="VFX94_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768932.1"
                     /translation="MKTPQIFIAYAPRGAGLRCALAYLVAERDVYGWYTGPRLEGTLA
                     GEFFGLEDFYSKHAARYSAVQVSDLHSNWALEEARRHELAQMQEAFAREWLVYRDDPR
                     AAVQLEAYAEAELAAGEVMLRFDRLAKLSKLQPNWTFYSAGFERSVLRHLAKRWPLEY
                     CIELAEAA"
     gene            2495..2710
                     /locus_tag="VFX94_11915"
     CDS             2495..2710
                     /locus_tag="VFX94_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768933.1"
                     /translation="MKIHVQCRHGGDPRTFLVGARRLQVMRVLERVAENSLRRFRVRV
                     FDGREFVLRHDLHTDDWRLASVRARQR"
     gene            2787..3041
                     /locus_tag="VFX94_11920"
     CDS             2787..3041
                     /locus_tag="VFX94_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768934.1"
                     /translation="MSKADIEVAAALKDLFRRLPSVVGFSVQEVLERELVVADLETHF
                     WPERSQELFAEVTVALLDLIDKEPAARELLIGRTFARTLH"
     gene            3136..>3389
                     /locus_tag="VFX94_11925"
     CDS             3136..>3389
                     /locus_tag="VFX94_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768935.1"
                     /translation="MTEDQVRLEAEAERATALLQGGHDYYVPLAVAAAIAFHQAHGST
                     KAIVSRDDYDDALNLAAAALSRLLTIYVVRDARQGRVPLA"
BASE COUNT          543 a         1163 c         1125 g          558 t
ORIGIN      
        1 tggaagagca tgcgcgcgcg cgtggcgcgc ccctccgacg gcgagctgtt ggccacgagc
       61 gcgacttccg tgccggacac tggagcttca ggcgaggctg cgcccgcctg gaactcctct
      121 tccgccagga tctcgcccga ggcctcgagc cagttctcgg cgaacgaagt ctgcagacct
      181 tccgctagct cgccgcagac ccgaatcatc gtgtcgcgcc acggctggcc ccggccaggc
      241 gggccgatcc agtagtccgc cacgcccatg ccgccgatga acgccacctc gccgtcgacg
      301 atgagcagat cgcggtgcgt gcggttgttg agacgcttca acgtgtacca ggcgatcggt
      361 tggtaccagt gcagctcgcc tccggcctgc ttcagcccgt cgaagtagcg cttcggggtg
      421 agcaggctgc catagcggtc cacgaccagc cgcacgcgca cgccgttgcg agcacgctcc
      481 tccagcgccg cgagcatttc gtcagcgacg cgcccgcgca agaagagata gacctcgaga
      541 tgaatgctgc gccgcgcggc gcggatggcg gcaagctgcg ccgcgaacgc ctgctcgccg
      601 ccgttcagta cgagcacctc gccgggcgcg aacacacgcg cgttcacgat cgccgagagg
      661 tagttcacga actcgcgcga cccgacgcca gcggcgggcg tgcgcacgcg atacgcgacc
      721 ggcggctcga acagcacgag aaagagcagc accgcgagga tcaggatcgc ggcaagctcg
      781 acggcgtcac ctagggaaac gggcagcact cagcgtatcc tcgccatcgc gctattttgc
      841 ccggctaggt aagctctgcc cgtgaagacc ttcgccgcct acaccgcagc cctcgcgatc
      901 gggctcgcga ctgccgtatt catcctctgg ctcgggcgca gcacgccggt cggtaccgac
      961 atctccttcg ctgccgcctc ggtcgtgttc gcactcgcca ttgtcgcgcg tgtgctttcc
     1021 gcgccgctcg tgcaccactg gttcggcctg gcgctcgcac tttcgctgcc gagctgcatc
     1081 ctcggcatcg tcatgttcag cctcgtggcg cggctcggag agtacttctg ggcctggctt
     1141 tggtcggcgc tcggcgcgct ggccgcgtcg ctcatcggag cgtacttcgc cgcgagggcg
     1201 agccgcgcct gatgcgcttc agtccggtgc gcacggccag cccgacggcg gcttcaaccc
     1261 cggcggcagc cgggtccggg cgtcgccgca ggcggcgtcg agctgcgcct gccggagccc
     1321 cttgaccgcc gagaggtcgg tgccttcgac gttggtcgcg aagaggtgag cttgggagaa
     1381 gtcggcgccg gcgaggttcg cttcgcgcag actcgcgcgc gcgaggctcg cgtatttgaa
     1441 gttcgcttcg cgcagatcgg cgcggtcgaa cactgagcgc ccgaaatcgc ccttggacat
     1501 gtcaacgccg cgcagcgcgg cgccggtcag gacgcagcgg tgcagctccg ccttctgcag
     1561 atcggcaccg acgagccgcg caccggcgaa gctgctgcgc accgcggtca gcttgctcag
     1621 gttcgcgcgc tcgagcacgg cgtcgcggaa cgaggtgcgg ctcacatgcg cggcggcgaa
     1681 gcttccgccg ctcagatcgc tgcgctcgaa gttgacggca agcagagtgc cgcgctcgaa
     1741 gcgcgcgcct tgcaggttct taccgcggat gacgagctgc tgtttggtgc aacccgacca
     1801 gtcgacgccc gcggatggcg cgtcgctgca ggccgcgtac gcgccaggct ccacgaggag
     1861 catcattgcg gcaaaggcca agctgcggtt gatcatgcga gatcgcttca ggctgcctcg
     1921 gcgagctcga tacagtactc tagcggccag cgcttggcca gatggcgcag caccgagcgc
     1981 tcgaagcccg ccgagtagaa ggtccagttc ggctgcaatt tggaaagctt ggcgaggcga
     2041 tcgaaccgca gcatgacttc gccggcggcg agctcggcct cggcgtaggc ctctagctgc
     2101 accgcggcgc gcgggtcgtc gcgatacacg agccattcgc gcgcgaacgc ttcctgcatc
     2161 tgcgcgagct cgtggcggcg tgcctcctcg agcgcccagt tcgagtgcaa gtcggagact
     2221 tggactgccg agtagcgtgc ggcgtgcttg gagtagaagt cctccagccc gaagaactcg
     2281 ccggcgagcg tgccctcgag gcgtggtccg gtataccagc cataaacgtc gcgctcggcg
     2341 acgaggtagg cgagagcgca gcgcagcccg gcgccgcgcg gcgcatacgc gataaagatc
     2401 tgcggagtct tcatgccgct atttgacgcc agcagatgcg cgcgtgcttg accgggatca
     2461 aggtccggcg ccaaagcgaa ggcaggatga agcgatgaag attcatgtgc agtgccggca
     2521 cggcggcgat ccccgcacgt ttctcgtcgg cgcgcgccgc ctgcaggtga tgcgcgtcct
     2581 ggaacgggta gccgagaact cgctacggcg cttccgcgtg cgggtgttcg acgggcgcga
     2641 gttcgtgctg cgtcacgacc tgcacaccga cgactggcgt ctcgccagcg tgcgcgcgcg
     2701 acagcgctag cgtttcggcg ttttcctacc ctgagaatca gagcgcgccg atagccgcag
     2761 gcgtccacgc cgcgtaacgt gaaccgatga gcaaagccga catcgaagtc gccgccgccc
     2821 tcaaagatct tttccgccgg ctgccctccg tcgtgggttt ctcggtacag gaggttctcg
     2881 agcgcgagtt ggtcgtcgcc gacctcgaaa cccacttctg gccagagcgt tcgcaggagc
     2941 tcttcgccga ggttacggtg gcgcttctcg acctcataga caaggagccc gccgcgcgcg
     3001 agctgctgat tggaagaact ttcgcgcgta cgcttcacta atagaggttt cttatccgcg
     3061 agtccgggct tcgtaagggc ttgcaccgcg gcccggtcgg gtagacactc ttcgcagccg
     3121 ccggacggag cggccatgac cgaagaccag gttcgtctcg aagccgaagc agagcgtgcc
     3181 accgcgcttc tccagggcgg gcacgattac tacgtgcctc tcgccgtcgc cgcggccatc
     3241 gcattccacc aggcgcatgg cagcaccaag gccatcgtca gccgcgacga ctacgatgac
     3301 gccctcaacc tcgccgccgc ggccctatcg cggctcctca cgatctacgt ggtgcgcgat
     3361 gctcgccagg gccgcgtgcc gctcgcgat
//
LOCUS       DASUNT010000588         2122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_428728, whole genome shotgun sequence.
ACCESSION   DASUNT010000588 DASUNT010000000
VERSION     DASUNT010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428728"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VFX94_11930"
     CDS             complement(<1..348)
                     /locus_tag="VFX94_11930"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5768936.1"
                     /translation="MPPVTQALLFTNIAAFLLSGLGVLPLDDLALWQPGGFESRFAPW
                     QLVTYAFLHGGLAHIFFNMLGLYMFGSEVERLFGSRFYAAYYFGCVLSAALCHLAVTG
                     WMGVPPYPTVGASG"
     gene            complement(350..1189)
                     /locus_tag="VFX94_11935"
     CDS             complement(350..1189)
                     /locus_tag="VFX94_11935"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX5768937.1"
                     /translation="MAFLSQTTPAFDLQGHRGARGHAPENTLPGFERALAIGVDTLEL
                     DVGVTRDGVVVIHHDRRLNPDIARGPDGRWIAAPGPTIHSLTYAELQRYDVGRTRPGS
                     EYAKQFARQQPLDGTRIPRLADLFELVKATPVRFNIETKVSPDAPDETPAPEPFARAV
                     LAEVRKAGVEERTTIQSFDFRTLAVVQREAPGISTAYLTSAKKGESIPKMVHEAKGAV
                     WSPNFRDLDARALAASRELGLRVIPWTVNEPADIARILEMKVDGIISDYPDRVREALK
                     ARR"
     gene            complement(1207..1584)
                     /locus_tag="VFX94_11940"
     CDS             complement(1207..1584)
                     /locus_tag="VFX94_11940"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5768938.1"
                     /translation="MQLDWDSGLVKHLGIRFVQTEKDRIVAELEIRPELLTTTGALHG
                     GTLMAFADTIGAAGTVVNLEPGQSTATLESKTNFFAAAFNGVVRAESTPLHKGRRTHV
                     WQTRITDASGKLLSLTIQTQVIL"
     gene            complement(1587..>2122)
                     /locus_tag="VFX94_11945"
     CDS             complement(1587..>2122)
                     /locus_tag="VFX94_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5768939.1"
                     /translation="KQVFAPPYLKAGKLVIAQTANILLYLGPRLGLAPRSEAHRLRLH
                     GLQLTIADWLSEVHDTHHPIASSLYYEDQKREAKRRAGIFLAERLPKFMRYFERTVDE
                     RFSYVHLSLFQMIEGLRYAFPNAMRKHERGYPRLLRIHDRVAARPRIAPYLASKRRIA
                     FNRHGIFRRYPELDERG"
BASE COUNT          362 a          670 c          733 g          357 t
ORIGIN      
        1 gcccgaggcg ccgaccgtcg gatagggcgg cacgcccatc catccggtga cggcgaggtg
       61 gcacagcgcc gcgcttagca cgcagccgaa gtaatacgcg gcatagaagc gcgagccgaa
      121 cagccgctcc acctccgaac cgaacatgta aaggccgagc atgttgaaga agatgtgcgc
      181 gaggccgccg tgcaggaagg cataggtaac gagctgccac ggcgcgaagc gcgactcgaa
      241 tccgcccggc tgccacagcg ccaggtcatc gagcggcagc acgccgagcc cgctcaggag
      301 gaacgccgcg atattcgtga acagcagcgc ttgggtgacc ggcggcaact cagcgcctcg
      361 ctttcagcgc ttcgcgcacc cggtccggat agtcgctgat gatgccgtcg accttcatct
      421 cgagaatgcg ggcgatgtcg gccggctcgt tcacggtcca ggggatgacg cgcagcccga
      481 gctcgcgtga ggcggccaac gcgcgcgcat cgaggtcgcg gaagttgggc gaccagaccg
      541 cgccctttgc ttcgtgcacc atcttcggaa tcgactcgcc cttctttgcc gaagtgagat
      601 aggcggtgct gatgccggga gcttcgcgct gcactaccgc gagggtacga aagtcgaacg
      661 actggatcgt cgtccgctcc tccacgccgg ccttgcgcac ctcggcgagc accgctcgcg
      721 cgaacggctc cggcgccggc gtctcgtcgg gcgcgtctgg cgaaaccttg gtctcgatgt
      781 tgaaacggac cggagtcgcc ttcaccagtt cgaacaggtc cgccaggcgc gggatgcgcg
      841 tgccgtcgag cggctgctgg cgggcgaact gcttcgcgta ctccgacccc ggccgggtgc
      901 ggccgacgtc gtagcgctgc aattccgcgt acgtcagcga atggatcgtt ggcccgggcg
      961 cggcgatcca gcgcccgtcc ggaccgcgcg cgatatccgg gttcaggcgg cgatcgtggt
     1021 ggatgacgac gaccccgtcg cgcgtcacgc cgacgtcgag ctcgagcgta tcgacgccga
     1081 tggcgagcgc gcgctcgaag cccggcagcg tgttctccgg cgcatggccg cgcgcgccgc
     1141 gatggccctg cagatcgaaa gccggtgtgg tttgagagag aaaagccatc aacagcagga
     1201 cctggtttat agaatcacct gggtctggat ggtgagcgaa agcaacttgc cgctcgcatc
     1261 cgtgatgcgc gtctgccaca cgtgtgtgcg cctgcccttg tgcagcgggg tcgactcggc
     1321 gcgcaccacg ccgttgaacg ccgcggcgaa gaagttggtc ttcgactcga gtgtcgcggt
     1381 gctctgcccg ggctcgaggt tcaccaccgt gccggcggcg ccgatcgtgt ctgcgaacgc
     1441 catcagcgtt ccgccatgca gcgcgccggt ggtcgtgagg agctcgggac ggatctcgag
     1501 ctcggccacg atgcggtcct tttccgtctg cacgaagcgg atgccgaggt gcttcaccag
     1561 tccgctatcc cagtcaagct gcatgctcag cctctctcgt cgagctcagg atagcggcgg
     1621 aagatgccgt gccggttgaa cgcaatgcgc cgcttcgagg cgagataggg cgcgatgcgc
     1681 ggccgggcgg cgacgcggtc atggatgcgc agcagcctgg ggtagccgcg ttcatgcttg
     1741 cgcatggcat ttggaaacgc gtaacgcagt ccctcgatca tctggaaaag cgaaaggtgc
     1801 acatacgaga agcgctcgtc taccgtgcgt tcgaagtagc gcatgaattt gggcaggcgc
     1861 tcggcaagga agatgccggc gcgccgcttg gcttcccgct tctggtcctc gtagtagagg
     1921 cttgacgcga tgggatggtg ggtgtcgtgc acctcgctca gccagtccgc gatggtgagc
     1981 tgcaggccgt ggagtcgcag acggtgcgcc tcgcttctcg gcgcgagacc gaggcgaggg
     2041 ccgaggtaca gcaggatatt cgcggtctga gcgatgacga gcttccccgc ctttaagtaa
     2101 gggggcgcga acacctgctt gt
//
LOCUS       DASUNT010000589         2629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1305297, whole genome shotgun sequence.
ACCESSION   DASUNT010000589 DASUNT010000000
VERSION     DASUNT010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305297"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFX94_11950"
     CDS             <1..189
                     /locus_tag="VFX94_11950"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEX5768940.1"
                     /translation="GSEVCGPEFTPDGRTLFIAVQHPGDGSTFDKPSTRWPDFRNDLP
                     PRGAVIAVTKADGGEIGG"
     gene            191..1153
                     /locus_tag="VFX94_11955"
     CDS             191..1153
                     /locus_tag="VFX94_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5768941.1"
                     /translation="MKRSLVFLLALFSTALFAQSFPSKPMRVLVPFPAGGTTDVVARL
                     VGQRVSEQVGQPVLVENRPGAGGTIAAAEVARAAPDGYTLLIHNITFPIASVAAALGN
                     RATYNADTDFAGVSIAVYVPFVWTAHPSTVGKDLSETMKYLQKEKSVRYTYGSTGPGS
                     TMHVLGEALSRAAGVEMTHVPFKGAAPLKQDLIAGRINLGGDQLSTSLADIKAGKLKA
                     LATSASRRIAELPDVPTVRELGYPVLEAEGWNGFFAPAKTPREVLERLNREFAAAAKH
                     PEVAKRLREMAAEPVGSPGAEQEAIFKRQMDQFRPVIKEMRIEG"
     gene            1157..2107
                     /locus_tag="VFX94_11960"
     CDS             1157..2107
                     /locus_tag="VFX94_11960"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5768942.1"
                     /translation="MRKIFLGLLFVCGTAVAQSWPTKPVKLIVTYPPGGSSDLMSRVY
                     GAKLGELWGQQVVIESKPGAAGSIGMDFAAKQPHDGYTFVVGNLGPVAVNPLLSKVPY
                     NIEKDFVAVSMISTGPNVLVVRTDAPFQSLKEVIAFAKANPGKLNYGTSGPGSVSHLS
                     SEMLKNITKVDAVEVAYKGGVLAVQDLLGGQINFIFSDSLPAMQHIRAGKLRALCVTG
                     TQKYELLPEPPPCQNDAPGLVAVNWWGVLFPAGTPRPIVQKLNEDTVKALADPDVKKR
                     FADLGVEAVSSTPEHFAGFIKEEMAKYAKLIKEANIKVNP"
     gene            2104..>2629
                     /gene="maiA"
                     /locus_tag="VFX94_11965"
     CDS             2104..>2629
                     /gene="maiA"
                     /locus_tag="VFX94_11965"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEX5768943.1"
                     /translation="MKLYGFWRSLASYRVRVALAMKGLRAEEISIDLLKGKQNTDEYL
                     AVNPQGVVPALVLDDDGPPLFQSLAILEFLEETRPTPPLLPNDARGRARVRGLSLIAA
                     ADGHPLITPRIRTYLEKEMRQDEPARTRWLAHWTLRALEAIDVHLSREKETGKFCHGD
                     TPTMADICLASQVIG"
BASE COUNT          465 a          874 c          875 g          415 t
ORIGIN      
        1 ggctccgagg tctgcggtcc cgagttcacg cccgacggca ggaccctctt catcgccgtg
       61 cagcatcctg gcgacggctc tactttcgac aagccgtcga cgcgctggcc ggacttccgc
      121 aacgacctgc cgcctcgcgg agcggtgatc gcggtgacca aagccgacgg cggcgaaatc
      181 ggaggatgaa atgaaaagaa gcctggtatt ccttcttgcg ctcttctcca ccgcgctctt
      241 cgcgcagagt tttccttcca agccgatgcg cgtcctggtg cccttcccgg ccggcggcac
      301 gaccgacgtc gtggcgcggc tcgtcggcca gcgcgtgagc gagcaggtgg gccagccggt
      361 gctggtggaa aaccgccccg gcgcgggcgg cacgatcgct gccgccgagg tcgcgcgcgc
      421 ggcgcccgac ggatacacgc tcctcatcca caacatcacg ttcccgatcg cttcggtcgc
      481 cgcagcgctt ggcaaccgcg cgacatacaa cgcggacacg gatttcgccg gcgtgtcgat
      541 cgcggtctac gtgccgtttg tctggaccgc ccacccgagc acggttggca aggatctttc
      601 cgagacgatg aaataccttc aaaaggaaaa gtccgtgcgc tacacctacg gctccaccgg
      661 gccgggctcg accatgcacg tgctcggcga ggcgctctcg cgcgcggccg gggtcgaaat
      721 gacgcacgtg ccgttcaagg gcgcggcgcc gctcaagcag gacctgatcg ccggccgcat
      781 caacctcggc ggcgaccagc tttccacttc gctggcggac atcaaggcgg gcaagctgaa
      841 ggcgcttgcc accagcgcct cgcgccgcat cgccgagctg cccgacgtgc cgacggtgcg
      901 cgagctcggc tacccggtgc tggaagccga gggctggaac ggattcttcg cgccggcaaa
      961 gacgccgcgc gaggtcctcg agcggctgaa ccgcgagttc gccgcagcgg cgaagcaccc
     1021 ggaggtggcg aagcgcctgc gcgagatggc cgccgagccg gtcggctccc cgggcgccga
     1081 gcaggaagcg atcttcaagc ggcagatgga ccagttccgg ccggtcatca aagagatgcg
     1141 catagagggc taagccatga gaaagatttt tctcggattg cttttcgttt gcgggaccgc
     1201 ggttgcgcaa tcgtggccca ccaagccggt gaagctgatc gtcacgtatc cgcccggcgg
     1261 cagctcggac ctgatgtcgc gcgtctacgg cgcgaagctc ggcgagctct ggggccagca
     1321 ggtggtgatc gaatccaagc ccggcgccgc ggggtcgatc ggcatggatt tcgcggccaa
     1381 gcagccgcac gacggctaca ccttcgtcgt gggcaacctc ggcccggtgg cagtgaaccc
     1441 gctgctctcc aaggtgccgt acaacatcga gaaggatttc gtggcggtgt ccatgatctc
     1501 caccgggccg aacgttctgg tggtgcggac cgacgcgcct ttccagtctt tgaaagaggt
     1561 gatcgctttc gccaaggcga atcccggaaa gctcaactat ggcacgagcg gcccgggcag
     1621 cgtgtcgcac ctttcaagcg agatgctgaa gaacatcacc aaggtcgacg cggtcgaggt
     1681 ggcctacaag ggcggcgtgc tggcggtgca ggacctgctc ggcgggcaga tcaacttcat
     1741 cttctccgac tcgctgcccg caatgcagca tatccgcgcg ggcaagctgc gcgcgctgtg
     1801 cgtcacgggg acgcaaaaat acgagctcct gcccgagccg ccgccgtgcc agaacgacgc
     1861 gccggggctc gtcgcggtca actggtgggg cgtgcttttc cccgccggca cgccgcggcc
     1921 gatcgtgcag aagctgaacg aagacaccgt caaggcgctc gccgatccgg acgtgaagaa
     1981 gcgtttcgcc gacctcggcg tcgaggcggt gtcgagcacg ccggagcact tcgccggctt
     2041 catcaaggag gagatggcga agtacgcgaa gctcatcaag gaggcgaaca tcaaggtgaa
     2101 tccgtgaaac tctacggatt ctggaggtcg ctcgcgagct atcgcgtgcg ggtggcgctc
     2161 gcgatgaagg gcctgcgcgc ggaggagatc tcgatcgatc tcctcaaggg caagcagaac
     2221 accgacgagt acctggcggt gaacccgcag ggcgtggtcc ccgcgctcgt gctcgacgac
     2281 gacggtccgc cgctcttcca gtcgcttgcg atcctcgaat tcctcgagga gacgcggccg
     2341 accccaccgc tcctgccgaa cgatgcccgc ggccgcgcgc gcgtgcgcgg gttgtcgttg
     2401 atcgccgccg ccgatggcca tccgctgatc acgcctcgga taagaaccta cctcgagaag
     2461 gagatgaggc aagacgagcc ggcccgcacc cgctggctcg cccactggac gctgcgcgcg
     2521 ctcgaggcga tcgacgtgca cctctcgcgg gagaaggaga ccggcaagtt ctgccacggc
     2581 gacacgccga cgatggcgga catctgcctc gcttcccagg tgatcggcg
//
LOCUS       DASUNT010000590         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1305341, whole genome shotgun sequence.
ACCESSION   DASUNT010000590 DASUNT010000000
VERSION     DASUNT010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305341"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VFX94_11970"
     CDS             <1..436
                     /locus_tag="VFX94_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768944.1"
                     /translation="ENQRALTALLENLNRLAYNADRNLAGLDRTIASVGAAAEDVRAL
                     SRALGEDASRLSTRYDKLGEEATAAAREAAAAMRQVSADVARISQRVDALLASGDVEL
                     RITAQELRATADALGVVARRLNDPRSVLWGPPEGGLGPGEAR"
     gene            433..1023
                     /locus_tag="VFX94_11975"
     CDS             433..1023
                     /locus_tag="VFX94_11975"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEX5768945.1"
                     /translation="MRVFVLIILTVLTGCVSPGARQPQHFYVLEDIGKTFEARAARAS
                     TLLLAPTTAAGFYDTQNIAYSRSPGTRAYYHYHSWTELPARRIGELLAARLEKSGGFR
                     MVASMGTPVRGDVVLTTHLAELYHDAAAAPGVARIVLAAELTDPLKRAIVARKSFNAT
                     APAASFDAPGAVQGFNQALAGLLDEVSAWIDAEAPR"
     gene            complement(1040..>2126)
                     /locus_tag="VFX94_11980"
     CDS             complement(1040..>2126)
                     /locus_tag="VFX94_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5768946.1"
                     /translation="NLVSDGAPALALGMEKGDKDIMKHPPRPPKEPVINRDMAIGIGV
                     IALVDALAILTVFYFALQRYPTSLEAAQTIAFVTLCVSELVRAFTARSEYHSIFSIGV
                     FSNKWMVWAVGASLLLVLLVVYVPFLQPFFDTVPLTLDDWLMMAPFFFASAIAMELLK
                     LYFRWRSAKAFERRPAAAAAASAPVDTAGGASMTKVLIPVDGSRNSEFAVRHVVRQFM
                     NNTAMEIHLLNVQQPLSSYVTRFVSRKNVRDYHHDEAEKALRPARQILDSFGIPYAAH
                     AELGPRAETITDAARRLRCDHIVMSAARKNSLTRLVENSTTNRVLELTPVPVEIVAGD
                     SVSKWERYGIPASVGTLIALLVAAAAD"
BASE COUNT          345 a          721 c          749 g          311 t
ORIGIN      
        1 ggagaaccag cgtgcgctga cggcgctcct cgagaacctc aaccgcctgg cgtacaacgc
       61 cgaccgcaac ctcgcggggc tcgaccgcac tatcgcctcg gtgggcgccg cagccgagga
      121 cgtgcgcgcg cttagccgcg cgctcggcga ggacgcgagc cgcctgagca cccgctacga
      181 caagctcggg gaagaggcca ccgcggcggc acgcgaagcc gccgcagcca tgcgccaggt
      241 gagcgcggac gtggcgcgca tttcgcagcg cgtggacgcg ctgctcgcga gcggcgacgt
      301 ggagctgcgc attacggcgc aggagctgcg cgccaccgcc gacgcgctcg gcgtcgtggc
      361 gcgcaggctc aacgatccgc gcagcgtcct gtggggaccg ccggagggcg gcctaggtcc
      421 cggggaggcg cggtgagagt ttttgttctc atcatcttga cggttctgac cggttgcgtg
      481 tcgcctgggg cgcggcagcc gcagcacttc tacgtccttg aagacatcgg aaagaccttc
      541 gaagccaggg cggcgcgggc ttccacgctg ctgttggcgc cgaccaccgc cgccgggttc
      601 tacgacacgc agaacatcgc gtacagccgc tccccgggga cccgcgccta ttaccactac
      661 cacagctgga ccgagctgcc ggcgcgcagg atcggcgagc tgctcgccgc gcgcctggaa
      721 aaaagcggcg gctttcgcat ggtggctagc atgggaaccc cggttcgcgg cgatgtcgta
      781 ctgacgacgc acctcgccga gctttaccat gacgccgccg ccgcgccggg cgtggcgcgg
      841 atcgtgctcg ccgccgagct caccgacccg ctgaagcgcg ccatcgtcgc gcgcaagagc
      901 ttcaacgcca cggcacccgc agcgagcttc gacgcccccg gcgccgtgca gggattcaac
      961 caggcgctcg ccgggctgct cgacgaggtg agcgcctgga tcgacgcgga ggcgccgcgc
     1021 tagccgctcg gcttcacgct caatcggctg cggcggcgac gagcagcgcg atcagggtgc
     1081 cgacgctcgc cgggataccg tagcgctccc atttcgacac cgagtccccg gccacgatct
     1141 cgaccggcac cggggtcagc tcgagcaccc ggttggtggt cgagttttcc accaggcgcg
     1201 tgagcgagtt cttacgtgcc gcgctcatca cgatgtggtc gcagcgcagc cgccgggcgg
     1261 cgtcggtgat ggtttcggcg cgcggcccca gctcggcgtg cgcggcgtag gggatgccga
     1321 agctgtcgag gatctggcgc gcggggcgca gcgccttctc ggcctcgtca tggtggtagt
     1381 cgcgcacgtt cttgcggctc acgaaacgcg tcacgtagct actgagcggc tgctgcacgt
     1441 tcagcaggtg gatctccatc gcggtgttgt tcatgaactg gcgcacgaca tgccgcacgg
     1501 cgaactcgct gttgcgcgag ccgtcgaccg ggataagcac cttcgtcatg gaagcgcctc
     1561 ctgcggtgtc aaccggcgcg gacgccgcag cggcggctgc cggacgacgc tcgaatgcct
     1621 tcgcggagcg ccagcggaag tagagcttga ggagctccat ggcgattgcg gaggcgaaga
     1681 agaacggcgc catcatgagc cagtcgtcga gcgtgagggg cacggtgtcg aagaagggct
     1741 gcaggaacgg cacgtacacc acgagcagca cgagcagcag cgacgcgccg accgcccaca
     1801 ccatccactt gttggagaaa accccgatcg agaagatcga gtggtactcg gagcgcgcgg
     1861 tgaaggcgcg cacgagctcc gacacgcaga gcgtgacgaa ggcgatcgtc tgcgccgcct
     1921 cgaggctcgt cggatagcgc tgcagcgcga agtagaacac cgtcaggatg gcgagcgcgt
     1981 ccacgagcgc gatcacgccg atgccgatcg ccatgtcgcg gttgatcacc ggctccttcg
     2041 gcgggcgcgg cgggtgcttc atgatgtcct tgtcgccctt ctccatgccg agcgcgagcg
     2101 cgggcgcgcc gtcgctcacc aggttg
//
LOCUS       DASUNT010000591         2548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1129005, whole genome shotgun sequence.
ACCESSION   DASUNT010000591 DASUNT010000000
VERSION     DASUNT010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129005"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VFX94_11985"
     CDS             complement(<1..308)
                     /locus_tag="VFX94_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5768947.1"
                     /translation="MKPVWIVDDDRSIRWVIEKALSREGIEFNSFASAQDALDALGAG
                     TPEVLVSDIRMPGRSGLELLQAVKQRHPAVPVIVMTAYSDLDSAVAAFQGGAYEYLPK
                     P"
     gene            complement(305..1333)
                     /gene="glnL"
                     /locus_tag="VFX94_11990"
     CDS             complement(305..1333)
                     /gene="glnL"
                     /locus_tag="VFX94_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEX5768948.1"
                     /translation="MAAATFAGLDLLATAVVILDDEFVVRYANPAAENLLTAGARSLI
                     GQPFLGLFAERNELERSLEEARVIHWDYSARNVTYVRTGREPVPLSCTVTRIDAFGLG
                     LLAELRPIEHELRLAREERLESEQSANRELIRNLAHEIKNPLGGLRGSAQLLERELDR
                     AELREYTQVIIKEADRLQSLMDRMLTPHRAPRVEALSLHEVLERVRSLERAEFGIDIE
                     RDYDPSLPELMGDREQLIQAVLNIARNAAQAGARSITFRTRALRNVTILKQRHKLALE
                     LQVIDDGSGVPEEIQDRIFNPLVSGREGGTGLGLSLAQTFVHYHQGVIEFESKPGRTT
                     FRILLPLR"
     gene            complement(1357..1824)
                     /locus_tag="VFX94_11995"
     CDS             complement(1357..1824)
                     /locus_tag="VFX94_11995"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX5768949.1"
                     /translation="MDARKLLVALALGSLAMPAHAQLTEIFKCRDARGHWTYTNDRRQ
                     AEKEKCELVASQINVSPAQKAARPSNFPRESSAERAAGKGRQREILQQELASEEAALS
                     KARQALAEQEAVRSGDERNYARVEERLKPFKESVESHQKNIEALRREIANQAR"
     gene            complement(1858..>2548)
                     /gene="glnA"
                     /locus_tag="VFX94_12000"
     CDS             complement(1858..>2548)
                     /gene="glnA"
                     /locus_tag="VFX94_12000"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451619.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX5768950.1"
                     /translation="YTVWNTAASYGKTATFMPKPVVGDNGSGMHCHQSVWKGGKNLFA
                     GNGYAGLSDFALHYIGGIIKHAKALNAITNPGTNSYKRLVPGFEAPINLAYSARNRSA
                     ACRIPYVSSPNARRVEVRFPDPTANSYLAFAAMLMAGLDGVQNKIHPGDPIDKDLYHL
                     PPEEAKKVPNVCSSLEQALENLQDNHAFLTRGGVFSEDFIESYIALKNDELTRFRMTT
                     HPVEFDMYYAA"
BASE COUNT          381 a          834 c          874 g          459 t
ORIGIN      
        1 ggcttgggca ggtactcgta ggcgccgccc tggaacgccg ctacggcgct gtccagatcg
       61 gagtaagcgg tcattacgat caccggcacc gcgggatggc gctgcttcac ggcctgcagc
      121 agctcgagcc ccgagcgtcc cggcatgcgg atgtcggaga ccagcacctc gggggtgccc
      181 gcgccgagcg catcgagcgc gtcctgcgcc gaggcgaacg aattgaactc gatgccctcc
      241 cggctgagcg ctttctcgat cacccagcgg atggagcggt cgtcgtcgac gatccagacc
      301 ggtttcatct cagcggcagc aagatcctga atgtggtgcg gcccggtttg gactcgaact
      361 cgatgacgcc ctggtgatag tggacgaacg tctgagcgag cgagagtccc aagccggtgc
      421 ccccttccct tcccgagacc agcggattga agatccggtc ctggatctcc tccggcacgc
      481 ccgagccgtc gtcgatgact tgcaattcta atgccagctt gtggcgctgc ttgaggatgg
      541 tgacgttgcg cagcgcgcgc gtgcggaaag tgatgctgcg cgcgccggcc tgcgcggcgt
      601 tgcgcgcgat gttcagcacc gcctggatga gctgctcgcg gtcgcccatc agctcgggca
      661 ggctcgggtc gtagtcgcgc tcgatgtcga tgccgaactc ggcgcgctcg aggctgcgca
      721 cccgctcgag cacctcgtgc aggctcagcg cctcgacgcg cggcgcgcgg tgcggggtga
      781 gcatgcggtc catcagcgat tgcaggcggt cggcttcctt gatgatcacc tgggtgtact
      841 cgcgcaattc ggcgcgatcc agttcgcgct cgagcagctg cgcggatccg cgcaggccgc
      901 cgagcgggtt cttgatctcg tgggccaggt tgcggatcag ctcgcggttg gcgctctgct
      961 ccgattcgag gcgctcctcg cgcgcgaggc gcagctcgtg ctcgatcggc cgcagctcgg
     1021 ccagcaggcc caggccgaag gcgtcgatgc gcgtcacggt gcacgaaagc ggcaccggct
     1081 cgcggccggt gcgcacgtag gtgacgttgc gcgccgagta atcccagtgg atgacgcgcg
     1141 cctcctccag cgagcgctcg agctcgttgc gctcggcgaa caagccgagg aacggctgcc
     1201 cgatcaggct gcgcgccccg gcggtgagca ggttctcggc ggccgggttg gcgtagcgca
     1261 ccacgaactc gtcatccagg atgaccaccg cggtggcgag caggtcgagg ccggcaaacg
     1321 tagccgcggc cattcactct ggcctcaaaa cgaacttcag cgagcctggt tggcgatctc
     1381 gcgccgcagc gcctcgatgt tcttctggtg gctctcgacg ctttccttga acggcttcaa
     1441 ccgctcctcg acgcgcgcat agttgcgctc gtcgccgctg cgcaccgcct cctgctcggc
     1501 gagcgcctgg cgcgccttgg agagcgcggc ctcctcgctg gcgagctcct gctgcaggat
     1561 ctcgcgctgg cgccccttgc cggcggcgcg ctcggcgctc gactcgcgcg gaaaattgga
     1621 tgggcgggcg gccttctgcg ccgggctgac gttgatctgg ctcgccacca gctcgcactt
     1681 ctccttctcc gcctggcgcc ggtcgttggt gtaggtccaa tgcccccgcg cgtcgcggca
     1741 cttgaagatc tccgtgagct gggcatgcgc cggcatggct aacgagccaa gcgccagcgc
     1801 caccagcagc ttccgggcat ccatccccgg aagcttagca aaatcaatga gaaggcgcta
     1861 agcggcgtag tacatgtcga actccaccgg gtgcgtggtc atgcggaagc gggtgagctc
     1921 gtcgttcttc agcgcgatgt aggactcgat gaaatcctcc gagaacacgc cgccgcgcgt
     1981 gaggaaggcg tggttgtcct gcaggttttc cagcgcctgc tcgagcgacg agcacacgtt
     2041 gggcaccttc ttcgcctctt cgggcggcag gtggtagagg tccttgtcga tcgggtcgcc
     2101 gggatggatc ttgttctgca ccccgtcgag gccggccatc agcatcgccg cgaaggcgag
     2161 ataggagttg gccgtcggat cgggaaagcg cacctcgacg cggcgcgcgt tcgggctcga
     2221 aacgtacggg atgcggcagg ccgccgagcg gttgcgcgcc gagtaggcga ggttgatcgg
     2281 cgcctcgaag cccggcacca ggcgcttgta cgaattcgtc cccgggttgg tgatcgcgtt
     2341 cagcgccttg gcgtgcttga tgatgccgcc gatgtagtgc agcgcgaagt ccgacaggcc
     2401 ggcgtagccg ttgccggcga acaggttctt gccgcccttc cagaccgact ggtggcagtg
     2461 catgcccgag ccgttgtcgc cgaccacagg tttcggcatg aaggtcgcgg tcttcccgta
     2521 agacgcggcg gtattccata ccgtgtac
//
LOCUS       DASUNT010000592         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_690739, whole genome shotgun sequence.
ACCESSION   DASUNT010000592 DASUNT010000000
VERSION     DASUNT010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690739"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFX94_12005"
     CDS             complement(<1..186)
                     /locus_tag="VFX94_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5768951.1"
                     /translation="MPTIALLSWEQARAHASPVRFSVFVEEQGVPREIEIDEQDSSSV
                     HAVVFDGEAPVATGRLLP"
     gene            210..2174
                     /locus_tag="VFX94_12010"
     CDS             210..2174
                     /locus_tag="VFX94_12010"
                     /inference="COORDINATES: protein motif:HMM:NF006196.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HEX5768952.1"
                     /translation="MKSRWSDAEASRFTGPLGARVYTSRLLGADKSLVLHGGGNTSVK
                     LREKDIFGEERDVLYVKGSGWDLETIEPEGFVPLPLAYMQRLPSLASMSDAQMVNELN
                     THVLRAGAPSPSVETLLHALLPHRYVDHTHADALLAVSNAPEGEKRMRDIYGERVAVI
                     PYAMAGFQLAALFARAFPRQAGAKTIGVVLLSHGIVSFGAEARESYERMIELVSLAEA
                     YLERKRAWHLPPGEAKSFPVQREAIAGLRRAISEQAGFALVMKVDGSQKFRAFAQRPD
                     VEKLSQHGPATPDHLLFTKPVPMIGRDAAGYARRYREYFERNAGAEGKTMLDPAPRMV
                     LDRELGFAAAGRDAHEAAIIEELYDHTIDVILGAEALGGWRSVGERHLFDIEYWELEQ
                     AKLRKSGPRPPFRGEVAVVTGAASGIGKACVQAFLRRGAAVAGLDRNPAIENAWRQPD
                     FLGATVDLTDPTALGEALDRTVRTFGGVDMVVLNAGIFPSSQPLEEVTAQTWQETMSV
                     NVEAPLRILQLCHPLLRLAPNGGRIVVIGSRTVPAPGPGAAAYSASKAALNQLARVAA
                     LEWAQDGIRINSLHPDAVYDTGLWTPELLAARAKAYNMTVEQYRRKNLLKTEVSSRDV
                     AELAAEMCGPLFAKTTAAQIPVDGGNDRVV"
     gene            2175..>2403
                     /gene="mtnA"
                     /locus_tag="VFX94_12015"
     CDS             2175..>2403
                     /gene="mtnA"
                     /locus_tag="VFX94_12015"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466012.1"
                     /note="isomerizes methylthioribose-1-phosphate into
                     methylthioribulose-1-phosphate; involved in methionine
                     salvage pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX5768953.1"
                     /translation="MSVEPLRWLGDRLELLDQRQLPDNTVYVTCRTAAEVAQAIRDMV
                     VRGAPAIGCAAAFGIALDPAVSSYDILAKSRP"
BASE COUNT          387 a          818 c          816 g          382 t
ORIGIN      
        1 cggcaaaaga cggccggtgg ccaccggtgc ttctccgtcg aagacgacgg cgtgaaccga
       61 gctgctgtcc tgctcgtcga tctcgatctc gcgcggcacg ccctgctctt cgacgaacac
      121 gctgaagcgg accggcgagg cgtgcgcacg cgcttgctcc caggagagca gcgcgattgt
      181 cggcatggcg cgaaaagtat aatcgccgga tgaaaagccg ctggagcgac gccgaagcgt
      241 cgcggttcac cggcccgctc ggcgctcgcg tctatacctc gcgcctgctc ggcgccgaca
      301 aatcgctcgt cctgcacggc ggcggcaaca cctcggtgaa gctgcgcgag aaggacatct
      361 tcggcgaaga gcgcgacgtc ctctacgtga aaggcagcgg ctgggatctc gagaccatcg
      421 agcccgaggg cttcgtgccg ctgccgcttg cctacatgca gcggctgccc tcccttgcgt
      481 cgatgagcga tgcgcagatg gtgaacgagc tcaacactca cgtgttgcgc gcgggcgcgc
      541 cctcgccttc ggtggagacg ctgctgcatg cgctcctgcc gcaccggtac gtggaccaca
      601 cgcacgcgga tgcgctactc gccgtgtcca acgcgcccga gggcgagaag cgcatgcgcg
      661 atatctacgg cgagcgcgtc gccgtcatcc cttacgccat ggcgggattc cagctcgccg
      721 cgcttttcgc gcgcgcgttc ccgcgccagg ccggcgccaa aacgatcggc gtggtgctcc
      781 tctcgcacgg catcgtttcg ttcggcgccg aggcgcgcga gtcctacgag cgcatgatcg
      841 agctcgtctc cttggccgag gcttacctcg agcgcaagag ggcttggcac ttgcctcccg
      901 gggaagccaa gagcttcccg gtgcagcgcg aggcgatcgc aggcctgcgg cgcgcgatct
      961 ccgagcaggc cggatttgcc ttggtcatga aagttgacgg cagccaaaaa ttccgtgcgt
     1021 tcgcgcagcg accggacgta gagaagcttt cgcagcacgg cccggccact ccggaccacc
     1081 tcctcttcac caagccggtg ccgatgatcg ggcgcgatgc ggccgggtac gcccggcgct
     1141 atcgcgagta tttcgagcgc aacgccggcg cggaaggaaa aaccatgctc gatccggcgc
     1201 cgcgcatggt gctcgaccgc gagctcggct tcgccgccgc cgggcgcgac gcgcacgagg
     1261 cggcgatcat cgaggagctc tacgaccaca ccatcgacgt catcctgggc gccgaggcgc
     1321 tcggcggctg gcgctctgta ggcgaaaggc acctgttcga catcgaatac tgggagctcg
     1381 agcaggcgaa gttgcgcaag tcgggcccga ggccgccgtt tcgcggcgag gtggccgttg
     1441 tgacgggagc agcgtcgggc attggcaagg cgtgcgtgca ggcgttcctg cggcgcggcg
     1501 cggcggtggc gggcctcgac cgcaacccgg ccattgaaaa tgcgtggcgg cagcccgatt
     1561 ttttgggcgc gaccgtggat ctcaccgacc ccacggcgct cggcgaggcg ctggatcgta
     1621 ccgtgcgcac cttcggcggc gtggacatgg tcgtgctcaa cgccggcatc ttcccctcct
     1681 cgcagccgct ggaggaggtc acggcgcaga cctggcagga gacgatgagc gtgaatgtag
     1741 aagcgccgct gcgcatcctg cagctgtgcc atcccctgct tcgcctcgcg ccgaacggcg
     1801 gacgaatcgt ggtgatcggt tcgcgcactg tcccagcgcc gggacccggt gcggctgcct
     1861 attcggcgtc caaggcggcg ctcaaccagc tggcgcgcgt cgccgcgctc gaatgggcgc
     1921 aggatggcat ccgtatcaac tcgcttcatc cggacgcggt gtacgacacc ggcctctgga
     1981 cgcccgagct cctcgcggcg cgcgccaagg cctacaacat gaccgtggag cagtaccgca
     2041 ggaaaaacct tctcaagacc gaagtgagtt cgcgcgacgt cgcggagctc gccgcggaga
     2101 tgtgcgggcc gctcttcgcc aagaccaccg ccgcgcagat cccggtggac ggcggcaatg
     2161 accgggtggt ctgattgagc gtcgagccgc tgcgctggct gggcgatcgc ctggagcttc
     2221 tcgaccagcg ccagcttccc gacaacacgg tctacgttac ctgccgcacc gcggctgagg
     2281 tggcgcaggc aatccgcgac atggtggtgc gcggcgcgcc ggccatcggt tgcgcggcgg
     2341 ccttcggcat cgcgctggac cccgcagtgt cctcctacga catcctggcg aagagccgtc
     2401 cta
//
LOCUS       DASUNT010000593         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1217204, whole genome shotgun sequence.
ACCESSION   DASUNT010000593 DASUNT010000000
VERSION     DASUNT010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217204"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VFX94_12020"
     CDS             <1..695
                     /locus_tag="VFX94_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5768954.1"
                     /translation="LVEGIGEDFIPPVCDLSRVRTAYSIPDKEAFRTCRDLLRKEGIM
                     AGTSSGTLIAAALRYCREQTQPKRVVSLVPDSGNKYLSKVYNDYWMIDQGFIERETYG
                     DLRDLIARRHKEHAVVTVNADETVLAAHQRMKLYDMSQRPVLQDGRIVGLVDEEDILL
                     EVYENPAHFSEPVTEAMESRLVTVPPSASIGQLMAIFKRGLVAIVVDGSEFLGLITRT
                     DLLNWLRRRTQQ"
     gene            692..1834
                     /locus_tag="VFX94_12025"
     CDS             692..1834
                     /locus_tag="VFX94_12025"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEX5768955.1"
                     /translation="MNFETKVIHAGQEPDPRTGAVMTPIYATSTYAQSSPGEHKGYDY
                     ARTRNPTRDALEACLAELEGGKAGFAFASGMAAIGTVLELLDSGSHVVAMDDLYGGSY
                     RILERVRKRSANISATFTDLSDPGNLEKNLKPNTRMVWVETPTNPLLKIVDLEALAAI
                     ARRHNVISVCDNTFASPWIQRPLELGFDIVVHSTTKYLNGHSDVIGGAAVLKEQTWKE
                     KLAFLQNAVGAVPSPFDAFLTIRGIKTLALRMERHCGNAMHVAAWLEKHPRVARVLYP
                     GLASHPQHTLAARQMNARYGGMVTAMLKGGLAASRRFLERCKLFTLAESLGGVESLIE
                     HPAIMTHASLPEDVRVGLGIDEGLVRLSVGIESVDDLIAELKYALD"
     gene            1899..>2124
                     /locus_tag="VFX94_12030"
     CDS             1899..>2124
                     /locus_tag="VFX94_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768956.1"
                     /translation="MIRMPGTSHAGPLPPLTGEARALSLRLRLHVAALAAKERNVDLE
                     TPARYIEQAFRAQGVEFSVQEFASGGRKVRN"
BASE COUNT          390 a          706 c          690 g          338 t
ORIGIN      
        1 ggctcgtcga gggcatcggc gaggacttca tcccgccggt ctgcgacctt tcgcgcgtgc
       61 gcacggccta cagcattccc gacaaggaag cgtttcgcac gtgccgcgac ctgctaagga
      121 aggaaggcat catggccggc acctcttccg ggacgctgat cgccgcggcg ctgcgctact
      181 gccgcgagca gacccagccg aagcgcgtgg taagcctggt tcccgacagc ggcaacaagt
      241 atctgtccaa ggtgtacaac gactactgga tgatcgacca gggcttcatc gagcgcgaga
      301 cctacggcga tctgcgcgac cttattgccc ggcgccataa agagcacgcg gtcgtcacgg
      361 tgaacgcgga cgaaaccgtg ctcgccgcgc accagaggat gaagctctac gacatgtcgc
      421 agcgtcccgt gctgcaggac ggcaggatcg tcggcctcgt ggacgaggaa gacatcctgc
      481 tcgaggtgta cgaaaatccc gcgcatttca gcgagccggt gaccgaggcg atggagagcc
      541 gcctcgtcac cgtgccgccg agcgcgtcga tcggccagct gatggcgatt ttcaagcgcg
      601 gcctggtggc gatcgtcgtg gacggcagcg agtttctcgg cctcatcacg cgcacggatc
      661 tgctcaactg gctgcgcaga aggacacaac aatgaacttc gaaaccaagg tgatccacgc
      721 cggacaggaa cccgatccgc gtaccggcgc cgtgatgacg ccgatctacg ccacctccac
      781 ttacgcgcag tcgagccccg gcgagcacaa gggctacgac tacgcgcgga cgcgaaaccc
      841 gacgcgcgac gcgctcgaag cctgcctcgc cgagctcgag ggcggcaagg cgggctttgc
      901 cttcgcctcc ggcatggccg cgatcggcac ggtgctcgag ctcctcgatt ccgggtcgca
      961 cgtggtggcg atggacgacc tctacggcgg gagctaccgg atcctggagc gcgtgcgcaa
     1021 gcgctccgcc aacatcagcg cgactttcac ggacctgtcc gatcccggaa atctcgagaa
     1081 gaatctgaag cccaataccc gcatggtctg ggtcgagaca cccacgaatc cgctcctcaa
     1141 gatcgtcgat ctcgaagcgc tcgcagcgat cgcgcgccgg cacaacgtga tctcggtgtg
     1201 cgacaacact ttcgcctcgc cgtggattca aaggccgctc gagctcggct tcgacatcgt
     1261 ggtgcactcc acgaccaagt acctcaacgg gcattccgat gtcatcgggg gcgccgcggt
     1321 gctcaaagag cagacctgga aggaaaagct ggcattcctg cagaacgcgg tcggcgcagt
     1381 gccgagcccg ttcgacgcct tcctgacgat acgcggcatc aagacgctcg cgctgcgcat
     1441 ggagcggcac tgcggcaatg cgatgcacgt cgccgcgtgg ctggagaagc atccgagggt
     1501 agcccgggtg ctgtatccgg gactggcgag ccacccgcag cacacgctcg cggcgcgcca
     1561 gatgaacgcg cgctacggcg gcatggtcac cgcgatgctg aagggcggcc tcgcggcttc
     1621 ccggcgcttt ctcgagcgct gcaagctttt cactctggcc gagagcctcg gcggcgtgga
     1681 aagcctgatc gagcacccgg cgatcatgac gcacgcctcg ctgcccgagg acgtgcgcgt
     1741 cgggctgggc atcgacgagg gcctggtgcg cctttcggtc ggcatcgaaa gcgtggacga
     1801 cctcatcgcc gagctgaaat acgcgctcga ctagagccat cggcctgcct ggcgcgttcc
     1861 ttgccctcgc gctcgggata gcggggtttc tctacttcat gatccgcatg cccggaactt
     1921 cgcacgccgg cccgttgccg ccgctcacgg gagaggcacg cgcgctgtcg ctcaggctgc
     1981 ggctgcatgt ggcggcgctc gccgccaagg agaggaacgt cgacctcgag acgcccgcgc
     2041 gttacatcga gcaggcgttt cgcgcgcaag gcgtcgagtt ctccgtccag gagttcgcga
     2101 gcggcgggcg caaggtgcgc aaca
//
LOCUS       DASUNT010000594         4576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1305573, whole genome shotgun sequence.
ACCESSION   DASUNT010000594 DASUNT010000000
VERSION     DASUNT010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305573"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VFX94_12035"
     CDS             <1..1143
                     /locus_tag="VFX94_12035"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEX5768957.1"
                     /translation="YYYLAAFSEAAMVGALADWFAVVALFRRPLNLPIPHTAIIPRNK
                     GRIARGLSEFIQQNFLSSAAVVQRIAEFKPGDTLCRWLLRSENVATVAHYATRFIGYA
                     LGAVDDERVQRFLQRNFTGLVRSADIAAAAAQVLDILTENKRHHALLDAALNSLDAVL
                     AKEETRRYIAVEVGKSAPLLKRISDWFNLQLDERAALKIAEVAIAKVHEVREDRDHEL
                     RRRFDDYVASFIDRLKKDDSLRRKVHAIRDELLESPALGSYIGGLWEEFREWLAADLN
                     LAASVTHERIAGMVRAFGTRLEADREIRQWIDEQILAAIPPLVEEHRAKIGRFVEDQI
                     NGWQEQKLVEELERHIGPDLQYIRVNGTLVGGLAGLVIASLTELAR"
     gene            complement(1140..1445)
                     /locus_tag="VFX94_12040"
     CDS             complement(1140..1445)
                     /locus_tag="VFX94_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768958.1"
                     /translation="MQKLLALALALSLQPAMATAPPVEIDPNTRIQGGADPRGSGAAA
                     GAGTPRDSKMDSDPRIEPPGHNAPRPDRADTDKDKPISERKPQEREDFERSKSQPGR"
     gene            complement(1721..2800)
                     /locus_tag="VFX94_12045"
     CDS             complement(1721..2800)
                     /locus_tag="VFX94_12045"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX5768959.1"
                     /translation="MKVHAIDTCVLTVPTPKPMALQYPQHKLVVAEISTDEGVRGLGY
                     SLVFGGGGAEAVQAYLDARLKPALLGEDPTYVEYLWEKMFRVDMGMKKQGAAAYALAA
                     LDIGLWDIAGKALGKPLYKLWGAMRHRIGCYGSGGWAKYTVEDLVREAEKYAGYGCKY
                     YKMKIHDPDPKVNRARVEAVRRALGPGVRLMVDVNQRLDVIGNQRQAALLEEFDLVWY
                     EEPVLADDLQALSEVARAIRIPVATGENNYTRYEFRDIIQRSGARYLMPDVCRANGFS
                     ETLKIGHLAAAHGLAVSPHVVHEISLHVVGALANGFLVEFMDWAPPDLFEELPECKGG
                     RFRIPDRPGHGISLARGAIDKYRSR"
     gene            complement(2813..3100)
                     /locus_tag="VFX94_12050"
     CDS             complement(2813..3100)
                     /locus_tag="VFX94_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX5768960.1"
                     /translation="MRQHAEFDAILAEYREQLPQKLARLESLFAGARFGELMRELHTL
                     AGSAGTFGLPRVGEAASAAEAYLDSCGSNPDSGQRAELARLLGLVRQASFY"
     gene            complement(3112..3840)
                     /locus_tag="VFX94_12055"
     CDS             complement(3112..3840)
                     /locus_tag="VFX94_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5768961.1"
                     /translation="MSDKRVYVVTSKGNAELTGANTSLSRDDLKLLVLVDGYASIADI
                     ARQTENNLPVAAVAQTLDRLAKAGFIADPDATASINVGDFFKESETGLASLQASGFYV
                     RIARRDPERPTLAKDHKVTVLAVEDDPALTKLLRTYLRMEGFNVRLAATREEIMKGLV
                     EQPRPDVVLLDVTLPDTDGFDVLTKMRSTEQVKDVPVIMTTAKATREAVLKGLRQGAN
                     GYVTKPFDMDIVLKAIRTVLGLKG"
     gene            3925..>4576
                     /locus_tag="VFX94_12060"
     CDS             3925..>4576
                     /locus_tag="VFX94_12060"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5768962.1"
                     /translation="MHIFGPPARFPLARERRYTPPEASLEALLALHERLGLERVLIVQ
                     PSVYGTDNSCTLDALRRLGPRARGVAVTDTRIRENELRRLKRLPVALVVDHFGLAQST
                     DEVRYLASLLREGNTYVKLSGPHACRWAPDAAALERLRGWTAMRAVSQNPRRHSSSPL
                     RFLDDPAHHRDRSLRGVAGAAAQERHAARAPCCRQDHGAYGLFSRRAVLRPRRQPLR"
BASE COUNT          707 a         1540 c         1522 g          807 t
ORIGIN      
        1 tactactacc tcgctgcgtt cagcgaggca gcgatggtgg gcgcactcgc cgactggttc
       61 gcggtggtgg cgctctttcg ccggccgctc aatctgccga tcccgcacac cgccatcatc
      121 ccgcgcaaca agggcaggat cgcgcgcggg ctgtcggaat tcatccagca gaatttcctc
      181 tcgtccgccg cggtagtgca gcgcatcgcg gaattcaagc cgggcgacac gctttgccgc
      241 tggctgctga ggagcgagaa cgtggctacg gtcgcgcact atgcgacccg cttcatcggc
      301 tatgcgcttg gcgccgtgga cgacgagcgc gtgcagcgct tcctgcagcg caatttcacc
      361 ggcctcgtgc gcagcgccga catcgccgcg gccgcggccc aggtgctcga tatcctgacc
      421 gagaacaagc gccaccatgc gctgctcgat gccgcgctca acagcctcga cgccgtgctc
      481 gcgaaggagg agacgcggcg ctacatcgcg gtcgaggtag gcaagagcgc gccgctcctg
      541 aagaggatca gcgactggtt caacctccag ctcgacgagc gcgcggcgct gaagatcgcc
      601 gaggtggcga tcgcgaaggt gcacgaggtg cgcgaagacc gcgaccacga gctgcgccgg
      661 cgcttcgacg actacgtcgc ttcattcatc gacaggctga agaaggacga ctcgctcaga
      721 agaaaggtgc acgcgatccg cgacgagctg ctcgaaagcc ccgcgctcgg cagctacatc
      781 ggcggcctgt gggaggagtt ccgcgaatgg ctcgcggcgg acctgaacct cgccgcgtcg
      841 gtgacgcacg agcgcatcgc cggcatggtg cgcgccttcg gcacgcgcct ggaggccgac
      901 cgcgagatcc gccagtggat cgacgagcag atcctcgccg cgatcccgcc gctggtggag
      961 gaacaccgcg cgaagatcgg gcgcttcgtc gaggaccaga tcaacggctg gcaggagcag
     1021 aagctcgtcg aggagctcga gcgccatatc ggccccgacc tgcagtacat ccgcgtgaac
     1081 ggcacgctgg tcggcgggct cgccggcctg gtgatcgcct cgctcaccga gcttgcgcgc
     1141 tagcggccgg gctggctctt gctcctttcg aagtcttcgc gttcctgcgg cttgcgctcg
     1201 ctgatcggct tgtccttgtc cgtgtccgcc ctgtccggac gcggagcgtt gtggccgggc
     1261 ggctcgatcc gcgggtcgct gtccatcttg ctgtcgcggg gcgtgccggc gccggcggcg
     1321 gcgccgctac cgcgcgggtc ggcgccgccc tggatcctgg tattcggatc gatttccacg
     1381 ggaggcgccg tggccatcgc cggctgaagc gaaagagcga gcgcgagcgc gaggagtttt
     1441 tgcatggcct cgaaattgct gcaagtgcca tgccatgaag ctcttgtgcc tcgcgcttct
     1501 gctgatcggg cttgccgcat gcgaacgcga catccctcag cgcagcggcg ggaacgaagg
     1561 cagcgcggcg caggcggccg gcggtgtcgg ctcggatgaa ggcagggccg cgcgtcgcgc
     1621 cgggcggtag gaggtccggg aataccagga cgaaggccta acttcgcagc tttgcgaagt
     1681 aagttagagg gctccgtccc cgaatccccg tcccgaattc ctacctgctg cggtatttgt
     1741 cgatcgcccc gcgcgcgagc gagatgccgt gccccggccg gtcggggatg cggaagcggc
     1801 cacccttgca ctcgggcagc tcttcgaaga gatcgggcgg cgcccagtcc atgaactcga
     1861 cgaggaagcc gttggcgagc gcgccgacca cgtggagcga gatctcgtgc acgacgtgcg
     1921 gcgatacggc gaggccgtgc gctgcggcga ggtggccgat cttgagcgtc tcgctgaagc
     1981 cgttggcgcg gcagacatcc ggcatgagat agcgcgcccc gcttctctga atgatgtccc
     2041 tgaactcgta acgggtgtag ttgttctcgc cggtggcgac cggaatgcga atcgcacgcg
     2101 ccacttcact cagtgcctgg aggtcgtcgg cgagcaccgg ctcctcgtac cagacgaggt
     2161 cgaactcttc gagcagcgcc gcctggcgct ggttgccgat cacgtcgagg cgctggttga
     2221 cgtccaccat cagccgcacg cccgggccga gggcgcggcg caccgcttcg acccgcgcgc
     2281 gattcacttt ggggtcgggg tcgtggatct tcatcttgta gtacttgcag ccgtacccgg
     2341 cgtacttttc ggcttcgcgc accaggtctt ccaccgtgta tttcgcccag ccgccgctgc
     2401 cgtagcagcc gatgcggtgc cgcatcgcgc cccaaagctt gtagagcggc ttcccgagcg
     2461 ccttgccggc gatgtcccac aggccgatgt cgagcgcagc cagcgcgtac gccgctgcac
     2521 cctgcttctt catccccatg tcgacgcgaa acatcttttc ccacagatat tcgacgtacg
     2581 tgggatcttc gccgagcagc gcgggcttga gccgggcgtc gaggtatgcc tgcaccgctt
     2641 ccgcgccgcc gccgccgaac accaggctgt agcccagccc gcgcacgcct tcgtcggtgc
     2701 tgatctcggc gacgaccagc ttgtgctgcg ggtactggag cgccatcggc ttcggcgtgg
     2761 ggacggtgag gacacacgtg tcgatggcgt gaactttcac ggcctgccga tgctagtaga
     2821 acgaggcctg acggacgagg cccagcagcc gcgcgagctc ggcgcgctgg ccggagtccg
     2881 ggttcgagcc gcacgagtcg aggtaggctt cggccgcgct tgccgcctcg cccacgcgcg
     2941 gcaggccgaa cgtgccggcc gacccggcga gcgtgtgaag ctcgcgcatg agctcgccga
     3001 agcgagcacc ggcgaagagg ctttccaggc gcgccagctt ctgcggcagc tgctcccgat
     3061 actctgcgag gatggcgtcg aactcggcgt gttgcctcat cgcgcgccgc gctagccctt
     3121 cagcccgagc accgtccgga tcgccttgag aacgatgtcc atgtcgaacg gcttggtgac
     3181 gtagccgttg gcgccctgcc gcaagccctt gagtacggct tcgcgcgtcg ccttcgcggt
     3241 cgtcatgatg acgggcacgt ccttcacctg ctcggtactg cgcattttcg tcagcacgtc
     3301 gaagccgtcg gtgtcgggga gcgtcacgtc gagcagcacg acgtcgggcc ggggctgctc
     3361 gaccagccct ttcatgattt cctcgcgcgt cgcggcaagg cgcacgttga agccctccat
     3421 gcgcaggtac gtgcgcagca gcttggtgag ggcggggtcg tcctcgaccg cgagcaccgt
     3481 gaccttgtgg tccttcgcga gcgtcgggcg ctccggatcg cgccgcgcga tgcgaacgta
     3541 gaagccgctt gcctgtagcg aggcgagccc ggtctcggac tccttgaaaa aatcgccgac
     3601 gttgatgctc gcggtggcgt ccggatcggc gataaagccg gctttcgcca gccggtccag
     3661 cgtctgcgcg acggccgcga ccggaaggtt gttctccgtc tgcctggcga tgtcggcgat
     3721 gctcgcgtag ccgtcgacca gcaccaggag cttgagatca tcccgcgaaa gcgaggtgtt
     3781 cgccccggtg agttcggcgt tacccttcga cgtcaccaca tagacgcgtt tgtcgctcac
     3841 gatcccttcc cccggtaact ataatctttt gccggaaatc cagataatgc ctttgcacgt
     3901 cccgccgggg gcttgcgact gcctgtgcat attttcggcc cgccggcgcg ctttccgctg
     3961 gcccgcgagc gccgctacac ccccccggaa gcctcgctag aagcccttct cgcactgcac
     4021 gaacggcttg gactggagcg cgtgctgatc gtgcagccga gcgtctatgg cactgacaac
     4081 tcctgcacac tcgatgccct gcgacgtctc ggcccgcggg ctcgcggcgt cgccgtcacc
     4141 gacactcgca tccgggagaa cgagctgcgg cgcttgaagc gcctgcccgt cgcgctcgtg
     4201 gtcgatcatt tcgggctggc gcaatcgacg gacgaagtac gctatctcgc gtcgctcctt
     4261 cgggaaggaa atacctacgt gaagctgtcc ggtccgcacg cttgccgatg ggccccggac
     4321 gccgcggcgc tcgagcgact gcggggctgg acggcgatgc gggctgtttc gcaaaatcct
     4381 cgtcgacatt ccagctcgcc tttacgattt ctagatgacc ccgcgcatca tcgagaccga
     4441 agtcttcgcg gagttgccgg cgcggctgcg caagagcggc acgccgcccg agcgccttgc
     4501 tgccggcaag accacggcgc ctatgggctc ttttctcgaa gggccgtcct tcgaccgcga
     4561 cggcaacctt tacgtg
//
LOCUS       DASUNT010000595         2696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_602838, whole genome shotgun sequence.
ACCESSION   DASUNT010000595 DASUNT010000000
VERSION     DASUNT010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602838"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..136
                     /locus_tag="VFX94_12065"
     CDS             <1..136
                     /locus_tag="VFX94_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEX5768963.1"
                     /translation="TRRLARYRCESCGFRARQFHWQCPACGGWETYPPRRTEEFDLTP
                     "
     gene            147..1457
                     /locus_tag="VFX94_12070"
     CDS             147..1457
                     /locus_tag="VFX94_12070"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX5768964.1"
                     /translation="MNVTVIGTGYVGLVTGACLADAGNHVFCLDVNEERIRALNDGHV
                     PIFEPGLEPIVKRNHDAGRLRFSTDVASSVAHGELQFIAVNTPPGEDGSADLQHVLAA
                     ARAIGRHMKAWKLVVTKSTVPVGTADRIGEVLKAETKVPFAVVSNPEFLKEGAAVQDF
                     MRPDRVVIGAQDERAVKLLRQLYAPFQRNHERIMVRSVRSAELTKYAANSMLATRISF
                     MNELANLAEAIGADIEEVRRGIGSDPRIGYQFLYAGAGFGGSCFPKDVKALQRSALGA
                     GKPLRVLAAVEAVNEAQKHVLAEKVKARLGKDLRGRTIAVWGLAFKANTDDMREAPSR
                     TLITDLVAAGALVRGYDPAAGEQARKIFESEKGIAVVDSALAALEGADALVIVTEWQE
                     FRSPDFAAIRERLKVPAIFDGRNLYDPAVLKGLGIEYYPIGRRT"
     gene            1454..2374
                     /gene="rfaE1"
                     /locus_tag="VFX94_12075"
     CDS             1454..2374
                     /gene="rfaE1"
                     /locus_tag="VFX94_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEX5768965.1"
                     /translation="METSKARVLVVGDVMLDRYWFGDVSRISPEAPVPVVLIQEEDQR
                     LGGAANVAWNCKELGARTRLLSVVGRDEPGQALERLLKARRIEASLHRDRGLSTTQKL
                     RVIGRRQQLLRIDFERPPSREVLALKLEEFKRALADCDVVILSDYGKGGLAHIVEMIR
                     SARRAGKRVLVDPKGDDYSRYRNASIITPNLAELRQVIGTWKDEKDLKSRAQALAGRL
                     GLEALLLTRGEDGMTLFKGSKTFSVKAEKREVLDVTGAGDTVIAALAVMLAAGAGLES
                     AVRLANRAAGIVVGKLGTAAVKHGELFGAR"
     gene            2378..>2696
                     /locus_tag="VFX94_12080"
     CDS             2378..>2696
                     /locus_tag="VFX94_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5768966.1"
                     /translation="MYCVVTGAAGFIGSKLVEGLNRHGITEVIAVDNLSHADKFRNLA
                     DAEIADYLDQAEFIANLERLEGSVEAVLHQGACSDTMESDGRYMLQNNYEYSKRLYEW
                     CQEE"
BASE COUNT          483 a          872 c          938 g          403 t
ORIGIN      
        1 cacgcggcgc ctcgcgcgct atcgttgcga gagctgcggc ttccgggcgc gccagttcca
       61 ctggcaatgt ccggcctgcg gcggctggga gacctacccg ccgcgccgca ccgaggaatt
      121 cgatctcaca ccatgaacaa ttccgcatga acgtaaccgt tatcggcacc gggtacgtcg
      181 gcctggtcac cggcgcctgc ctcgctgacg ccgggaacca cgtcttctgc ctcgacgtga
      241 acgaagaacg catccgcgcg ctgaacgacg ggcacgtccc gatcttcgag cccggcctcg
      301 agccgatcgt gaagcgcaac cacgacgccg gcaggctgcg gttctccacc gacgtggcgt
      361 caagcgtggc gcacggcgag ctgcagttca tcgcggtgaa cacgcctccc ggcgaggacg
      421 gctccgccga cctgcagcac gtgctcgccg ccgcgcgcgc catcgggcgg cacatgaagg
      481 catggaagct cgtcgtcacc aagtcgacgg ttccggtcgg caccgcggac cgcatcgggg
      541 aggtgctgaa agccgaaacg aaggtgccgt ttgccgtggt gtcgaacccg gagtttctga
      601 aggaaggcgc ggcggtgcag gacttcatgc gccccgaccg ggtcgtcatc ggcgcccagg
      661 acgagcgggc cgtgaagctg ctgcgccagc tctacgcgcc tttccagcgc aaccacgagc
      721 gcatcatggt gaggagcgtg cgctcggccg agcttaccaa gtacgcggcc aactcgatgc
      781 tcgcgacgcg tatctccttc atgaacgagc tcgccaacct cgccgaggcg atcggcgccg
      841 acatcgagga ggtgcgccgc ggcatcggct ccgatccgcg catcggctac cagttcctct
      901 acgccggcgc gggcttcggc ggctcgtgct tccccaagga tgtcaaggcg ctgcagagaa
      961 gcgcgctcgg agcaggaaag ccgctgcgcg tgctcgctgc agtggaggcg gtgaacgagg
     1021 cacagaagca cgtgctcgcc gagaaggtga aggcgcggct cggcaaggat ctgcgcggcc
     1081 gcacgatcgc ggtgtggggg ctcgcgttca aggcgaatac cgacgacatg cgcgaggcgc
     1141 cgagccgcac gctcataacc gacctggttg ccgccggggc tttggtgcgc gggtacgacc
     1201 ctgcagccgg ggagcaggcg aggaaaatct tcgagagcga gaaaggcatt gcggtggtcg
     1261 actcggcgct tgcggcgctg gaaggcgcag acgctctcgt catcgtcacc gagtggcagg
     1321 agttccgcag cccggatttt gccgccatcc gcgagcggct gaaggtgccg gcgatcttcg
     1381 acggccgcaa cctgtacgat cccgcagtgc tgaaaggcct cggcatcgag tactacccga
     1441 tcgggagaag aacatagaaa cctccaaggc ccgcgtcctg gtggtcggcg acgtgatgct
     1501 cgaccgctac tggttcggcg acgtgtcgcg catctcgcct gaggcgccgg tgccggtggt
     1561 cctgatccag gaggaggacc agcgactcgg cggcgcggcg aacgtggcgt ggaactgcaa
     1621 ggagctcggt gcgcgcacac gcctgctttc cgtggtgggg cgcgacgagc cggggcaggc
     1681 gctcgagagg ctgctcaagg cccggcgcat cgaagcgagc ctgcaccggg accgcggcct
     1741 gagcaccacg cagaagctgc gcgtgatcgg caggcgccag cagctgttgc gcatcgattt
     1801 cgagcgtccg ccctcgcgcg aagtgctcgc cctgaagctc gaggaattca agcgcgcgct
     1861 tgccgattgc gacgtggtca tcctgtcgga ctacggcaag ggcgggctcg cgcacatcgt
     1921 cgaaatgatc cgcagcgctc gccgggcggg gaagcgcgtg ctggtcgacc cgaagggcga
     1981 cgactattcg cgctacagga acgcgagcat catcacgccg aacctggccg agctgcgcca
     2041 ggtgatcggg acctggaagg acgagaagga cctgaagagc cgcgcccagg cgctcgccgg
     2101 caggctcggg ctggaagcgc tgctgcttac ccgcggcgag gacggcatga ccctgttcaa
     2161 gggaagcaag accttttcgg tgaaggccga gaagcgcgag gtgctggacg tcaccggcgc
     2221 cggcgacacg gtgatcgcgg cgctcgccgt catgctcgcc gcgggcgcgg gactggagag
     2281 cgccgtgcgc ctcgccaacc gcgctgccgg catcgtggtc ggcaagctgg gcaccgcggc
     2341 ggtgaagcac ggggaacttt ttggagctcg ctgatccatg tattgcgtag tcaccggcgc
     2401 cgccggattc atcggctcga agctcgtcga aggcctcaat cggcacggca tcaccgaggt
     2461 catcgcggtg gacaaccttt cgcacgcaga caagttccgc aacctggccg atgccgagat
     2521 cgccgattat ctcgaccagg ccgagttcat cgcgaacctc gagcgcttgg agggctcggt
     2581 ggaagcggtg ctgcaccagg gcgcctgctc cgacaccatg gagagcgacg ggcgctacat
     2641 gctgcagaac aactacgaat attcgaagcg cctctacgaa tggtgccagg aagaag
//
LOCUS       DASUNT010000596         5947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_866037, whole genome shotgun sequence.
ACCESSION   DASUNT010000596 DASUNT010000000
VERSION     DASUNT010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866037"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2038)
                     /locus_tag="VFX94_12085"
     CDS             complement(<1..2038)
                     /locus_tag="VFX94_12085"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX5768967.1"
                     /translation="MIDKSFNPAAIEQRWYERWESCGYFRHRDANLPGATPAYCIQLP
                     PPNVTGTLHMGHAFQQTLMDALIRYHRMRGYNTNWVAGTDHAGIATQIVVERQLEEQG
                     KTRHDLGRDKFVERVWQWKQQSGSSITRQMRRLGASANWTYADTEGQRAGYFTMDARM
                     SRAVVEVFVRLYEEGLIYRGKRLVNWDPVLGTAVSDLEVDTEEEDGRIWTIRYPLEER
                     SGSISVATVRPETMLGDVAVAVNPKDTRFTALVGKKAVLPLTGRTIPIIADDYVDPQF
                     GTGAVKITPGHDFNDYHVGKRHGLEAISVMTLDGRMNEQAPVKYRGLERFAARKQVIE
                     DLRKQDLLVADKPYKLRVPRSGRTGVIVEPMLTDQWFVAMESLARKGLEVVAKGEVRF
                     FPEHWTKTYTEWLENIQDWCISRQLWWGHRIPAWYDDKGNVLVGASEEEVRKKHNITA
                     PLKRDPDVLDTWFSSALVPFSSLGWPEKTKDQDLFLPSSVLVTGFDIIFFWVARMVMM
                     TTHFTGKVPFRHVYINAIVRDAEGEKMSKSRGNTLDPLDLIDGIEFKDLLDKSTSGLL
                     RKEHKEKAEKYVRSHFANGIPAFGADALRFTFASLATHGRDIKFDLQRCEGYKNFCNK
                     LWNAARFALMNCEGFQASGKPQPKTDAERWILQRLQHTLAQIAEGFADYRFDQAA"
     gene            complement(2035..2337)
                     /locus_tag="VFX94_12090"
     CDS             complement(2035..2337)
                     /locus_tag="VFX94_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768968.1"
                     /translation="MNDRDVPVLTEIVTEERALRRPVDDATLEALTRSLEQRLLERLG
                     PEVHRIVAERLARTLATTLDQAIEGLRSEIRLNLQQMVREAVAGCVASVPPPKNPE"
     gene            complement(2330..2746)
                     /locus_tag="VFX94_12095"
     CDS             complement(2330..2746)
                     /locus_tag="VFX94_12095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEX5768969.1"
                     /translation="MDFYFNAPDRLQVACRLAGKAMKENQRLLIYAPDAELATRIDKM
                     LWTWPATGFVPHCAVHAPLAAETPVLIASGDETPEACELLLNLGADCPPHFERFSRLL
                     EIVDATDREAGRKRYRFYRERGYKLANHDLAQGSNE"
     gene            complement(2752..4239)
                     /locus_tag="VFX94_12100"
     CDS             complement(2752..4239)
                     /locus_tag="VFX94_12100"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX5768970.1"
                     /translation="MKFSIKALSPESAKTGCVVVGVHQGTDQKGGELTAAARRLDQAS
                     KGALKQALRDISGRTGSTLLLRGLPGVAAERVLLVGLGEKPARETQYRDAVRAAGNAL
                     KDLGATDAALFLVDMKVAARPLGWNVRHAVLCTADAFYRFDRLKTQKKPPAPALAEVI
                     LPLSPKAELENALREAMATAGGSALARTLGNLPSNICTPSYLAEEAKKLAREFKLAVE
                     VLERKDMEKLGMGALMSVARASHEPPKLVVIKYQGRKGAKPLAFVGKGITFDTGGISL
                     KPAAEMDEMKFDMSGAAAVLGTVRALAEMRAPVNVVGIIPTCENMPGGHATKPGDVVT
                     TLSGQTVEILNTDAEGRLILCDALSYAERFEPEAVIDIATLTGAIVISLGHVATGLFA
                     NDEKLAAQVRAAADDAWDRVWQMPLWEDYQEQLRSNFADMANIGGRPAGSVTAACFLS
                     RFTKKLRWAHLDVAGTAWKSGREKGSTGRPVPLLVRFALRHAGRK"
     gene            4256..5332
                     /gene="lptF"
                     /locus_tag="VFX94_12105"
     CDS             4256..5332
                     /gene="lptF"
                     /locus_tag="VFX94_12105"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEX5768971.1"
                     /translation="MIFQRALLREFAGLAGAVFMTLFAIAVTTRLIRLLGQAAGGRFP
                     SDAVFAFLGFFALGALPVLLSLTMFISVLLTMTRSWRDSEMVIWFGAGQSLTAWLKPV
                     MLFALPQIAVIAALSLFISPWAAQMAAQYTSKLETRDDVSRVTPGVFGETANRTRVFF
                     VESVSGDSSAVQNIFVSSVQQSKSGVSMSRGGHTETAPNGDRFIVLEKGRRYEGAPGD
                     PEYRVTEFDRYAARIELKEGKEVTPTHKNLPTWALITHPTNQNLGELLWRVGIPISAG
                     VLVMLAIPMSFVNPRAGRSVNLLFALLTYIVYSNLLSVSQARVAQGRMDFGVGWWLVH
                     AGMIALLVFMFAQRMQLIRLRIGK"
     gene            5337..>5947
                     /locus_tag="VFX94_12110"
     CDS             5337..>5947
                     /locus_tag="VFX94_12110"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX5768972.1"
                     /translation="MRTLERYLARQIYFATGFVLLGFLGLFAFFDLIGELGDLGKADY
                     HLRQVFTFVLLSAPTHAYELFPVVVLIGTLYVLSQLASNSEYTVMRASGLSPRRAGIT
                     LARIGVAFVVLTFLIGEWIAPWAEEQAQKVKLRAMSSAIGQDLSSGLWFKDEGSFINV
                     REARQTNQLVGVRIYDFDADYRLQTITLAQRADYAGGGLWQLH"
BASE COUNT          882 a         1968 c         1998 g         1099 t
ORIGIN      
        1 gcgccgcctg gtcgaagcgg tagtcggcga aaccttccgc gatctgcgcg agcgtgtgct
       61 gcaggcgctg caggatccag cgctcggcat cggtcttcgg ctgcggcttg ccgctggcct
      121 ggaagccctc gcagttcatc agcgcgaagc gggcggcgtt ccacagcttg ttgcagaagt
      181 tcttgtagcc ctcgcagcgc tgcaggtcga acttgatgtc gcggccgtgc gtcgccagcg
      241 acgcgaacgt gaagcgcagc gcgtcggcgc cgaacgccgg gatgccgttc gcgaagtggc
      301 tgcgcacgta tttctcggcc ttttccttgt gctccttgcg aagcaacccc gaggtcgact
      361 tgtccagcag gtccttgaac tcgattccat cgatgaggtc gagcgggtcg agcgtgttgc
      421 ccctggattt cgacattttc tcgccctcgg cgtcgcgcac gatggcgttg atgtacacgt
      481 ggcggaatgg caccttgccg gtgaagtgcg tggtcatcat taccatgcgc gccacccaga
      541 aaaagatgat gtcgaaccct gtgacgagca ccgaggaggg caggaacagg tcctggtcct
      601 tggtcttttc cggccagccg agcgacgaga aaggcaccag cgccgaggaa aaccaggtgt
      661 cgagcacgtc cgggtcgcgc ttcaagggcg cagtgatgtt gtgctttttc cggacctctt
      721 cctcgctcgc gccgaccagg acattgcctt tgtcgtcgta ccaggcggga atgcgatggc
      781 cccaccagag ctgtcgcgag atgcaccaat cctggatgtt ctcgagccac tcggtatagg
      841 tcttggtcca gtgctcgggg aagaaccgga cttcaccctt cgccacgact tccaggccct
      901 tgcgggcgag gctttccatc gccacgaacc actggtcggt cagcatcggc tcgacgatga
      961 ccccggtgcg gcccgaacgc ggcacgcgca gcttgtaggg cttgtccgcg acgagaagat
     1021 cctgctttcg caggtcctcg atcacctgct tgcgcgccgc gaagcgctcg aggccgcggt
     1081 acttcacggg cgcctgctcg ttcatgcgcc cgtccagcgt catgacgctg atcgcctcca
     1141 gaccgtggcg cttgccaacg tggtagtcgt tgaaatcgtg cccgggggtg atcttgaccg
     1201 cgcccgtgcc gaactgcgga tcgacgtagt cgtcggcgat gatcgggatc gtccttcccg
     1261 tgagcggcag gactgccttc ttgcccacga gcgcggtgaa gcgcgtgtct ttcgggttca
     1321 cggccaccgc gacgtcaccg agcatggtct cgggccgcac ggtcgccacg ctgatcgaac
     1381 cgctgcgctc ttcgagcgga tagcggatcg tccagatcct gccgtcctct tcctcggtgt
     1441 cgacttcgag gtcggacacc gccgtgccga ggaccggatc ccagttgacc aggcgcttgc
     1501 cccggtaaat gagcccctct tcatagagac gaacgaaaac ttccaccacc gcgcgtgaca
     1561 tgcgcgcgtc catggtgaag tagccggcgc gctgcccttc ggtgtccgca taggtccagt
     1621 tcgccgatgc gccgaggcgg cgcatctggc gcgtgatgct cgagcccgac tgctgcttcc
     1681 attgccagac gcgctcgacg aacttgtcgc ggcccagatc gtggcgcgtc ttgccttgtt
     1741 cctcgagctg gcgctcgacg acgatctgcg tggcgatgcc ggcatgatcg gtgcccgcca
     1801 cccagttcgt gttgtaaccg cgcatgcggt gatagcggat gagcgcgtcc atcagcgtct
     1861 gctggaaggc gtgccccatg tgcagcgtgc cggtgacgtt cggcggcggc agctggatac
     1921 agtacgccgg tgtcgcgccc ggaagattcg cgtcgcggtg gcggaagtag ccgcagcttt
     1981 cccaacgctc gtaccagcgc tgctcgattg ccgccgggtt gaagctcttg tcgatcactc
     2041 cgggttcttc ggcggcggca cggacgccac gcagccggcg accgcctcgc gcaccatctg
     2101 ctgcaggttc agcctgatct cggagcgcaa tccctcgatc gcctggtcga gcgtggtggc
     2161 gagcgtgcgc gcgaggcgct ccgcgacgat gcggtgcact tcgggaccga ggcgctcgag
     2221 cagcctttgc tcgagcgagc gcgtgagcgc ctccagcgtg gcgtcgtcga ccgggcggcg
     2281 cagcgcgcgc tcttcggtca cgatctcggt aagcaccggc acgtcgcggt cattcattgc
     2341 tgccctgcgc caggtcgtgg ttcgcgagct tgtagccccg ctcacgatag aagcgatagc
     2401 gcttacggcc cgcctcgcgg tctgtggcat ccactatttc cagcaggcgc gagaagcgct
     2461 cgaagtgggg cgggcagtcg gcgccgaggt tgaggaggag ctcgcaggct tcgggagtct
     2521 cgtcgccgga agcgatcagt accggcgtct cggccgcaag cggcgcatgc accgcgcagt
     2581 ggggcacgaa cccggtggcg ggccacgtcc agagcatctt gtcgatgcgc gtcgcgagtt
     2641 ccgcgtcggg agcgtagata agcagccgct ggttctcctt catcgccttg ccggcgaggc
     2701 ggcaggcgac ctgcagcctg tccggcgcat tgaagtagaa atccacgctg gtcatttgcg
     2761 gcccgcgtgc cgcagcgcga aacggacgag aagcggaacc ggccggccgg tcgagccctt
     2821 ttcgcggccg ctcttccacg cggtgccggc gacgtcgagg tgcgcccagc gcagcttctt
     2881 ggtgaagcgc gaaaggaagc atgccgcggt cacgctgccg gcggggcggc cgccgatgtt
     2941 cgccatgtcg gcgaagttcg agcgcagctg ctcctggtag tcctcccaca gcggcatctg
     3001 ccagacgcgg tcccaggcgt cgtcggcggc ggcgcgcact tgagcggcga gcttctcgtc
     3061 gttggcgaag aggcccgtcg cgacatggcc gagcgagatg acgatcgcac cggtcagcgt
     3121 agcgatgtcg atcacggcct cgggctcgaa gcgctccgca taggagagcg cgtcgcacag
     3181 gatgaggcgg ccctcggcgt cggtgttcag gatctcgacc gtctggcccg acagtgtggt
     3241 caccacgtcg cccggcttgg tggcgtgccc gccgggcatg ttctcgcacg tcggaatgat
     3301 gccgaccacg ttgaccggcg cgcgcatctc cgcgagcgcg cgcaccgtgc cgagcaccgc
     3361 ggcggcgccc gacatgtcga acttcatctc gtccatctcg gcggccggct tgagcgagat
     3421 gccgccggtg tcgaaggtaa tgcccttgcc gacgaacgcg aggggcttcg cgcctttgcg
     3481 gccctggtac ttgatcacca cgagcttcgg cggctcgtgc gaggcgcgcg ccaccgacat
     3541 cagcgcgccc atgccgagct tctccatgtc cttgcgctcg agcacttcca cggcgagctt
     3601 gaactcgcgc gcgagcttct tcgcctcctc ggcgaggtag gacggcgtgc agatgttcga
     3661 gggcaggttg ccgagcgtgc gcgcgagcgc gctgccgccc gccgtcgcca tcgcctcgcg
     3721 cagcgcgttc tcgagctcgg ccttgggact cagcggcagg atcacttcgg cgagcgcggg
     3781 cgcgggcggc tttttctgcg tcttcagcct atcgaaccgg tagaaggcgt ctgctgtgca
     3841 cagcacggcg tggcgcacgt tccagccgag cgggcgggcg gcgaccttca tgtcgaccag
     3901 gaaaagcgca gcatccgtgg cgccgaggtc cttgagggcg ttgcccgccg cgcgcaccgc
     3961 gtcgcgatac tgcgtttcgc gggcgggctt ctcgccgaga cccacgagca gcacgcgctc
     4021 ggccgcgacg cccggcagcc cgcgcagcag gagcgtcgag ccggttcggc cggagatgtc
     4081 tcgcagcgct tgcttgagcg cgcccttgga ggcctgatcc aggcggcgcg ccgcggcggt
     4141 gagctcgccg cccttttgat cggttccctg atgcacgccc accaccacgc aaccggtctt
     4201 cgccgactcg ggcgagagcg cctttatgct aaatttcacg ggccgaaagt atcgcatgat
     4261 cttccagcgg gcgctgctca gggaattcgc aggtcttgcg ggcgcggtgt tcatgacgct
     4321 gttcgccatc gcggtcacca cgcgtctcat ccgcctgctc ggccaggccg ccgggggcag
     4381 gtttccctcc gacgcggtgt tcgcgtttct cggcttcttc gcgctcggcg cgctgccggt
     4441 gctgctctcg ctcaccatgt tcatctcggt gctgctcacg atgacgcgca gctggcgcga
     4501 ctcggagatg gtgatctggt tcggcgccgg ccagtcgctc accgcgtggc tgaagccggt
     4561 gatgctgttc gcgctgccgc agatcgcggt gatcgcggcg ctgtcgctct tcatctcgcc
     4621 gtgggcggcg cagatggcgg cccaatacac ctccaagctc gagacgcgcg acgacgtctc
     4681 gcgcgtcacg ccgggcgtct tcggcgagac cgcgaaccgc acgcgcgtgt tcttcgtcga
     4741 gtcggtgtcg ggcgattcca gcgcggtgca gaacatcttc gtgagctcgg tgcagcagtc
     4801 gaaaagcggc gtgagcatga gccgcggcgg gcataccgag acagcgccca acggcgaccg
     4861 cttcatcgtg ctcgagaagg gccggcgcta cgagggcgcg cccggcgacc ccgagtaccg
     4921 ggtgacggaa ttcgaccgct acgccgcgcg catcgagctg aaggaaggca aggaagtcac
     4981 gccgacgcac aagaacctgc cgacctgggc gctcatcacc catccgacca accagaatct
     5041 gggcgagctg ctgtggcggg tcggcatccc gatctcggcg ggggtgctcg tgatgcttgc
     5101 gatcccgatg agcttcgtca acccgcgcgc cgggcgctcg gtgaacctgc tgttcgcgct
     5161 gctcacctac atcgtgtact cgaacttgct cagcgtgagc caggcgcgcg tggcgcaggg
     5221 acgcatggac ttcggcgtcg gctggtggct ggtgcacgcc gggatgattg cgctgctcgt
     5281 cttcatgttc gcgcagcgca tgcagcttat tcgcctgcgc atcggcaaat aggcgcgtgc
     5341 gcaccctcga gcgctacctc gcccgccaga tctacttcgc caccgggttc gtgctgctcg
     5401 ggttcctcgg gcttttcgcg ttcttcgacc tgattggcga gctcggtgac ctcggcaagg
     5461 ccgactacca tctgcgccag gtcttcacct tcgtgctgct gtcggcgccg acgcacgcct
     5521 acgagctctt cccggtggta gtgctgatcg gcacgcttta cgtcctttcg cagctcgcaa
     5581 gcaactctga gtacaccgtg atgcgggcct cggggctctc gccgcggcgc gccgggatca
     5641 cgctcgccag gatcggggtc gcattcgtcg tgctcacctt cctgatcggc gaatggatcg
     5701 ctccctgggc ggaggagcag gcgcaaaagg tgaagctgcg ggcgatgagc tcggcgattg
     5761 gccaggacct gtcgtcgggg ctttggttca aggacgaggg ctcgttcatc aacgtgcgcg
     5821 aggcacgcca gacgaaccag ctcgtcggcg tgcgcatcta cgatttcgac gccgattacc
     5881 gcctgcagac gatcacgctt gcgcagcgcg ccgactatgc cggcggcggc ctgtggcagc
     5941 tgcatga
//
LOCUS       DASUNT010000597         2511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_253288, whole genome shotgun sequence.
ACCESSION   DASUNT010000597 DASUNT010000000
VERSION     DASUNT010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253288"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..101)
                     /locus_tag="VFX94_12115"
     tRNA            complement(17..101)
                     /locus_tag="VFX94_12115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(65..67),aa:Leu,seq:tag)
     gene            157..945
                     /gene="hpnC"
                     /locus_tag="VFX94_12120"
     CDS             157..945
                     /gene="hpnC"
                     /locus_tag="VFX94_12120"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEX5768973.1"
                     /translation="MAVRHYENFPVASLLLPAPLREPVQIIYRFARSADDFADEGDDA
                     PQERLRKLDDYRHQLTAPRDAVLRDVAKIIREHELPVHLFADLLDAFTQDVTKKRYAD
                     FPQVLDYCRRSANPVGRLLLHLFKRTNDSDLRASDAVCSALQLVNFWQDVELDYRKNA
                     RVYLPQDDMARFGVTERHLEAMLCDDAWRRLMAFQVERTRRLMLRGSGLGKSLPGRIG
                     LEIRATIQGGLRILEKIESAGYDVFRRRPKLRTYDWPLLVARAL"
     gene            955..1788
                     /gene="hpnD"
                     /locus_tag="VFX94_12125"
     CDS             955..1788
                     /gene="hpnD"
                     /locus_tag="VFX94_12125"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEX5768974.1"
                     /translation="MTPDEYCEQKAAASGSSFYYSFLFLPVERRRAITALYAFCREVD
                     DLVDETSDAQVAAAKLAWWRLEVANLFAGKAQHPVTKALAVFKDRYAIAQDRLNEIID
                     GMEMDLTQTRYLDWPGLERYCYRVAGAVGLLAAGIFGYREARTLDYARELGTAFQLTN
                     IIRDVGEDARKNRIYLPMAELKQFGVPAADILNARETPQFSRLMEFQAARARAYYDKA
                     MQALPDADRRAQRPGLIMAAIYRTLLGEIERDGFRVLTRRTSLTPLRKFWISWRTWVS
                     A"
     gene            1773..>2511
                     /locus_tag="VFX94_12130"
     CDS             1773..>2511
                     /locus_tag="VFX94_12130"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5768975.1"
                     /translation="MGKRVGSPVNAPVAIVGAGYAGMAAAVALAERGVRVAVFESGAV
                     PGGRARRVQISVEGRELSLDNGQHILVGACTELFRLMRTVGVASEALLRIPHELRYAD
                     GFAFRALWFPAPLGLAAGLLVTARVPVAERIGALRFIAALRRRKWRFEPDITVKELLD
                     AHRQNGRLRHYLWQPLCVSALNTPLGHASANIFLAALRDTLAGEGDAADLLLPRIDLS
                     RLFPEPAAEYVRARGGEIRLGQTVRDLA"
BASE COUNT          401 a          862 c          816 g          432 t
ORIGIN      
        1 atttaatcgc cggtactggt gcgaagggag ggactcgaac cctcatgcct tgcggcgtca
       61 gatcctaagt ctggtgcgtc tgccaattcc gccaccttcg ccccgttccg aaagagctga
      121 acgttatagc tgccgaatca acgtattata gccgccgtgg cggtgcgcca ttacgagaac
      181 ttcccggtcg cttcgctgct cttgcccgcg ccgctgcgcg agccggtgca aatcatctac
      241 cgcttcgcgc gcagcgccga cgatttcgcc gacgaaggcg acgatgcgcc gcaggagcgc
      301 ctgcgcaagc tcgatgacta ccgccaccag ctcaccgcac ctcgcgatgc ggtgctgcgc
      361 gacgtcgcaa aaatcattcg ggaacatgag cttccggtcc atctgttcgc cgacctgctc
      421 gacgcgttta cgcaggacgt gacgaagaag cgctatgccg atttcccgca agtgctggat
      481 tactgccgcc gctcggccaa ccccgtcggc aggcttcttc tgcacctgtt caaacgaacg
      541 aacgattcag acctgcgcgc ctcggacgcc gtctgctctg ccctgcagct cgtgaatttc
      601 tggcaggacg tggagctcga ctaccggaag aacgcccggg tctacctgcc gcaggatgac
      661 atggcgcgat tcggggtcac cgagcggcat ctcgaggcaa tgctctgcga cgatgcctgg
      721 cgccggctga tggcatttca agtggagagg acacggcggc tcatgctccg cggatcgggc
      781 ctggggaaaa gccttcccgg ccgcatcggc ctcgaaatcc gcgcgacgat ccagggcggc
      841 ctgcgcatcc tcgagaagat cgaaagcgcg ggctacgatg tgtttcgccg ccgcccgaag
      901 ctgcgtacgt acgactggcc gctcctcgtc gccagggctt tgtgagagcc cggcatgacc
      961 cctgacgagt actgcgagca gaaggccgcg gcgagcggct cgagcttcta ttactcgttc
     1021 cttttcctgc ccgtggagcg ccggcgcgcg attacggcgc tctacgcgtt ttgccgcgag
     1081 gtggacgacc tcgtggacga gacgagcgac gcgcaggtcg cggcggcgaa gctcgcgtgg
     1141 tggcgcctgg aggtcgcgaa cctcttcgca ggcaaggcgc agcaccccgt caccaaggcc
     1201 ctggcggtct tcaaggaccg ctatgcgatc gcacaggatc gcctgaacga gatcatcgac
     1261 ggcatggaga tggacctgac gcagacgcgc tacctcgact ggccggggct cgagcgctat
     1321 tgctaccgcg tggcgggcgc ggtcgggctg cttgccgccg gcatcttcgg ctaccgcgag
     1381 gcgcgcacgc tcgactacgc gcgcgagctc ggcacggcat tccagctcac caacatcatc
     1441 cgcgacgtgg gcgaggatgc gcgcaagaac cgcatctatc tgccgatggc cgagctcaag
     1501 cagttcggcg tgccggcggc ggatatcctg aacgcgcgcg agacaccgca gttcagccgc
     1561 ctgatggagt ttcaggcagc ccgcgcccgc gcttactacg acaaggcgat gcaggcgctc
     1621 ccggacgcgg accgccgcgc gcagcgtccg gggctcatca tggcggcgat ctatcgcacg
     1681 ctgctcggag aaatcgagcg cgacgggttc cgcgtcctca cgcggcgcac ctcgcttacg
     1741 ccgctcagga agttctggat ttcgtggcgc acatgggtaa gcgcgtaggc agcccggtga
     1801 acgccccggt cgccattgtc ggcgccggct atgcaggcat ggccgccgcg gtcgcgctcg
     1861 ccgagcgcgg cgtgcgagtc gcggtgttcg agtcgggcgc cgtgccgggc ggacgcgcgc
     1921 gccgggtgca aatctccgtc gagggccgcg agctcagcct cgacaacggc cagcacattc
     1981 tcgtcggcgc ctgcacggag cttttccgcc tgatgcgcac ggtcggcgtt gcgtccgagg
     2041 cgctgctgcg cattccgcac gagcttcgct acgccgacgg gttcgcgttt cgcgcactat
     2101 ggtttcccgc gccgctcggc ctggcagcgg gactgctcgt cacggcgcgc gtgcccgttg
     2161 ccgagcgcat cggcgcgctc aggttcatcg cggcactgcg caggcgcaag tggcgcttcg
     2221 agcccgacat cacggtgaag gagctactgg atgcgcaccg ccagaacggc cggctgcgcc
     2281 attacctttg gcagccgctt tgcgtgtcgg cgctcaatac cccgctcggc cacgcctccg
     2341 ccaacatctt tctcgcggcc ttgcgcgaca cgctcgccgg cgaaggcgac gcagccgacc
     2401 tgctccttcc ccgtatcgac ctctcgcggc ttttccccga gccggccgcg gaatacgtgc
     2461 gcgcacgcgg cggcgaaatc cgccttgggc agacggtgcg ggatctcgcc t
//
LOCUS       DASUNT010000598         2305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_953169, whole genome shotgun sequence.
ACCESSION   DASUNT010000598 DASUNT010000000
VERSION     DASUNT010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953169"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VFX94_12135"
     CDS             complement(<1..398)
                     /locus_tag="VFX94_12135"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5768976.1"
                     /translation="MNTIRLLLVWLAGLAAALAQEPPIIVAAVISQTGAQAELAADYA
                     KGIELWRDELNASGGLLGRRVELRLVDDASKAARASALYAGLIKEEKADLLIGPYGSA
                     ATLVAAGEAERARRVMVNGAGQAPVIHGRAP"
     gene            401..1756
                     /locus_tag="VFX94_12140"
     CDS             401..1756
                     /locus_tag="VFX94_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5768977.1"
                     /translation="MTLQERLADIVGAAHVLTEPADTKPYLTDWRKQYSAPAECVVRP
                     ASTQEVSQVVALCAHEGVAIVPQGGNTGLVGGSVPTGARREAVLAMGRMNRIRALDAL
                     NDTITVEAGCVLAAVQRAADEAGRLFPLSLAAEGSCQVGGNLSTNAGGVNVLRYGNAR
                     ELVLGLEVVLPDGRVWNGLRGLRKDNTGYDLKQLFLGAEGTLGVITAAVLRLYPRPSA
                     VATAWIALASPREAVELLAALRERLGERISAFELVSRACLDAVLVHVADIHDPLPAAH
                     PWYVLAQFADSGSPVELQERVEQALAEREAALAQSGEQARALWRIRESIPEAQFANVK
                     HDISIPISRIADFIDSAGRDLNASFVQTEIYCFGHVGDGNLHYNVGPERLLASRDAIN
                     RIVHGAVARFGGSISAEHGLGQLKREAIRELKPPLEMELMRKLKDALDPLGLMNPGKV
                     L"
     gene            complement(1753..>2305)
                     /locus_tag="VFX94_12145"
     CDS             complement(1753..>2305)
                     /locus_tag="VFX94_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5768978.1"
                     /translation="AHKFGARGLGIELDAALVEKSRQAARTAGVADKVSFVQGDVLVA
                     DISKATVVTAYLLPALMVKLQSRFIDELAPGTRIVSHAFAMTGWTPDATETVRLKSRH
                     PGQGDESRLHLWVVPAEVRGLWRGPGIEVRIEQNYQKIDVQGARNAKLSGRDISWDGF
                     TGRVEGNRIVGELSGRAVELTRR"
BASE COUNT          372 a          793 c          781 g          359 t
ORIGIN      
        1 ggcgcacgcc cgtggatcac gggcgcctgg cccgcgccgt tcaccatcac gcggcgcgcg
       61 cgctccgctt cgccggcggc gaccagagtc gcggcggagc cgtacggacc gatgagcaga
      121 tccgccttct cctccttgat caagccggcg tacagcgccg acgcgcgcgc ggccttcgac
      181 gcgtcatcga caagccgcag ctcgactctt ctgccgagca agccgcccga tgcgttgagc
      241 tcgtcgcgcc agagctcgat gcccttcgcg tagtcggcgg cgagctccgc ctgcgcaccg
      301 gtctgtgaaa tcaccgcagc aacgatgatg gggggctcct gcgctagcgc ggcggcgagg
      361 cccgctagcc aaaccaggag aagccgaata gtgttcaagg atgaccctgc aagaacgcct
      421 cgccgacatt gtaggggcgg cccacgtgtt gaccgagccg gcggatacga aaccgtacct
      481 cacagactgg cgcaaacagt actcggcgcc ggccgaatgc gtcgtgcggc cggcgagcac
      541 gcaggaggtt tcgcaggtgg tggcgctgtg cgcgcatgag ggcgtcgcaa tcgtccccca
      601 gggaggcaac accggcctgg tgggcggctc tgtgcccacc ggcgcgcggc gtgaggctgt
      661 actcgcaatg ggccgcatga accgcatacg ggcgctcgac gcgctgaacg acacgatcac
      721 cgtagaagcg ggttgtgtgc tcgccgcggt gcaacgggcg gccgacgagg ccgggcgcct
      781 tttcccactg tcgcttgcgg ccgagggaag ctgccaggtc ggcggcaacc tttccaccaa
      841 tgccggcggc gtgaacgtgc tgcgttacgg caacgcgcgc gagctggtgc tcggtctcga
      901 ggtcgtgctg cccgacggac gcgtatggaa cggcctgcgc ggcctgcgca aggacaacac
      961 cgggtacgac ctcaagcagc tcttcctcgg cgcggaaggc acgctcggcg taattacggc
     1021 cgccgtattg cgcctctacc cgcggccgag cgccgtggcc acggcctgga tcgcactcgc
     1081 gagtccgcgc gaggcagtcg agctgctcgc cgcgctgcgc gagcggctcg gcgagcgcat
     1141 ttcggctttc gagctcgtgt cgcgcgcgtg cctagatgcg gtgctcgtcc atgtggcgga
     1201 tatccacgat ccgctgccgg cggcgcatcc ctggtatgtc cttgcgcaat tcgccgacag
     1261 cggcagcccg gttgagctgc aggagcgggt cgagcaggcg ctcgccgagc gcgaggcggc
     1321 cctcgcgcaa agcggagagc aggcgcgcgc gctctggcgc atccgcgaaa gcattccgga
     1381 agcgcaattc gcgaacgtga agcacgacat ctcgattccg atctcccgga ttgcggactt
     1441 catcgattcc gccgggcggg acttgaacgc gtctttcgtg cagaccgaga tctactgctt
     1501 cgggcacgtg ggcgacggga atctccatta caacgtcggt ccagaaaggc tgcttgcaag
     1561 ccgcgacgcc atcaaccgca tcgtgcacgg cgcggtcgcg cgcttcggcg gctcgatctc
     1621 cgcggagcac gggctcggcc agctcaagcg cgaggcgatc cgggagttga agccgccgct
     1681 cgagatggag ctgatgcgaa agcttaagga cgcgctcgac ccgctggggc tgatgaaccc
     1741 cggcaaggtg ctctagcggc gcgtaagttc gaccgcgcgg ccggagagtt cgcccacgat
     1801 gcggtttccc tccacccggc ctgtgaaccc gtcccacgag atgtcccgcc ccgacagctt
     1861 cgcgttgcgg gcgccctgca cgtcgatctt ctggtagttc tgctcgatgc gaacttcgat
     1921 ccccgggccg cgccaaaggc cgcgcacttc ggcgggcacg acccaaagat ggagccggct
     1981 ctcgtccccc tgcccggggt ggcgcgactt gagcctcacg gtctcggtcg catccggcgt
     2041 ccagcccgtc atcgcgaagg cgtgcgagac gatgcgcgtt cccggagcca gctcgtcgat
     2101 gaagcgcgac tgcaacttca ccatgagcgc gggcaggaga tatgccgtca cgacggtcgc
     2161 tttggagatg tcggcgacga gcacatcgcc ctgcacgaag ctcaccttgt cggcgacccc
     2221 agcggtgcgg gcagcctgcc gcgacttctc caccagcgcc gcgtcgagct cgatgcccag
     2281 accgcgcgcg ccgaacttat gcgcc
//
LOCUS       DASUNT010000599         4142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_253422, whole genome shotgun sequence.
ACCESSION   DASUNT010000599 DASUNT010000000
VERSION     DASUNT010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253422"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFX94_12150"
     CDS             complement(<1..239)
                     /locus_tag="VFX94_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768979.1"
                     /translation="MKRFLFLVAFLAGCAAPPPAPEPGKPAQPDATVVTPAPKENIAV
                     AGLMETARADAAAGNLANAAAAIERALRIEPRNPRL"
     gene            complement(256..2547)
                     /gene="mrcB"
                     /locus_tag="VFX94_12155"
     CDS             complement(256..2547)
                     /gene="mrcB"
                     /locus_tag="VFX94_12155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HEX5768980.1"
                     /translation="MRRLLWCSLLAVTLLAAGVTLYLDIRVRTEFEGRRFALPARIYA
                     RPLELHAGLRIAQADVVEELRQLGYREAPPEGESGWYAKRENGLELALRPFVFWDGRQ
                     PAKRISVVFDKDKITALRDAGGAELPLARLEPLAIGGIYPAGNEDRVLVRLPDVPKHF
                     IQTLIAVEDRKFYAHAGFDVRGLARAARSLFTDRVQGGSTITQQLVKNFFLTPERTIQ
                     RKLTELAMAVLLELHYDKDEILETYLNEIYFGQDRDRAIHGVGLAAQFYFGKEVQHLS
                     VAESALLVGLIRGPAHYDPYRHPNRALERRNLVLREARDQKSITAEEYAQARAMPLNV
                     SKKTGMGTTPYPAFLQLVHRQLRRDYDEADLRTEGLRIFTTLDPRVQESAEAALSRRL
                     GQFDKERRFNREPGLEGAVVVTHPQTGEVQALIGGRDPRFRGYNRALDASRPVGSLLK
                     PAIYLTALSEPSKYTLLTPIDDGPFVWKSRGAPDWQPANYDRKFHGIVPLRTALAQSY
                     NAAAARLGTEIGIERVLDTVRRLGVERPMRPFASTLLGAVELSPLEVAQMYQTIASGG
                     FRSPLRAIREVTTQEGRPLTRYPLAVEQVFAPEPIYLLTAAMQEVVREGTAQGLNRFL
                     PPEIGVAGKTGTTDEQRDAWFAGFTGDRLAIVWVGYDDNRAAKLAGASAALPIWGDMM
                     AALGPEPLALARPERIELVWIDPQTGLRGGASCPGATEVPFVQGSAPQDRAPCSGSID
                     AAVEAVGQTVEKAKSWLERLFGR"
     gene            complement(2659..3609)
                     /locus_tag="VFX94_12160"
     CDS             complement(2659..3609)
                     /locus_tag="VFX94_12160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5768981.1"
                     /translation="MAAAAESRGRQASTPREIPARGWLDITRRVKDQIKRDRLSIIAA
                     GVAFYALLAIFPGLVALVAMYGLFADPAQIERHVAPLSGILPPEAAKIVLTQLHDLVS
                     TNRGALGLGAIAGLLVALWSASSAVRTLMEALNVAYDEEERRGLVRFYGTALILTVGG
                     VIAVILAIGLVVGLPAVLNFIGLSSLLETVVSLARWPILALLAIVAFAVIYRYGPSRA
                     QPRWRWVSWGATIAVALWIVGSALFSLYVTRFGNYNETYGAAGAVVILLMWFLLSSYA
                     ILIGAEINAEMERQTRKDTTSGQERQMGARRAYAADTVGE"
     gene            complement(3609..>4142)
                     /locus_tag="VFX94_12165"
     CDS             complement(3609..>4142)
                     /locus_tag="VFX94_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768982.1"
                     /translation="LQEGMTSVREGMTSVRDKTSQTMQSASETLASTKDRIAGNISSL
                     RERATHATDTARYQWERARGSVDYLVQEQPLALGAIGLAIGAILGAAAPRTRYEERLM
                     GDASRGLAEKAREMGSQQLERAQETVKQVAETVGQGSRPGAESQPGQPEGNVPLQQAQ
                     SEHPGPTAAGPDRKGAW"
BASE COUNT          606 a         1430 c         1418 g          688 t
ORIGIN      
        1 aggcgcgggt tgcgcggctc gatgcgcagc gcacgctcga tggccgcggc tgcgttggcg
       61 agattgcccg cggcggcatc ggcgcgcgcg gtctccatca ggccggccac cgcgatgttt
      121 tctttcggcg ccggggtcac caccgtcgcg tccggttgag cgggtttgcc gggctccggg
      181 gcgggcggcg gcgccgcgca cccggcgagg aaagcgacaa gaaagaggaa ccgtttcatc
      241 gaccgaggaa ccgtttcatc ggccgaagag gcgctcgagc cagctcttcg ccttctcgac
      301 cgtctgcccg accgcctcga ctgcggcgtc gatgctgccg gagcaggggg cgcggtcctg
      361 cggcgccgag ccctgcacga agggtacttc cgtggcgccg gggcagctcg cgccgccgcg
      421 aagcccggtc tgcgggtcga tccacacgag ctcgatgcgc tcgggcctgg cgagcgcgag
      481 cggctcgggc ccgagcgcgg ccatcatgtc gccccagatc ggcagcgcgg cgctcgcgcc
      541 ggcgagcttt gcagcccggt tgtcgtcgta gcccacccac acgatggcga gccggtcgcc
      601 ggtgaagccg gcgaaccagg cgtcgcgctg ctcgtccgtg gttccagtct tgccggcgac
      661 cccgatttcc ggcggcaaga acctgttcag gccctgcgcg gtgccttcgc gcactacttc
      721 ctgcatcgcg gctgtaagga ggtaaatcgg ctcgggcgcg aacacctgct cgaccgcgag
      781 cggatagcgc gtgagcgggc ggccctcctg cgtcgtgacc tcgcggatgg cgcgcagcgg
      841 agagcggaag ccgccgctcg cgatcgtctg gtacatctgc gccacttcga gcggggagag
      901 ctccaccgcg cccagcaggg tggaagcgaa cggccgcatc gggcgctcca cgccgagtcg
      961 cctgacggta tcgagcacgc gctcgatgcc gatctcggtc ccgaggcgcg ccgcggcggc
     1021 gttgtaggac tgcgcgagcg cggtgcgcag cggcacgatg ccgtggaact tcctgtcgta
     1081 gttcgccggc tgccagtcgg gcgcgccgcg gctcttccag acgaacggcc cgtcgtcgat
     1141 cggcgtaagc agcgtgtact tagaaggttc gctcagagct gtcaggtaga tggccggctt
     1201 caggagcgag ccgaccgggc gcgatgcatc cagcgcgcgg ttgtagccgc ggaagcgcgg
     1261 atcacggccg ccgatcagcg cctggacttc gccggtctgc gggtgggtca cgaccaccgc
     1321 gccctccagc ccgggctcgc ggttgaagcg gcgctccttg tcgaactgcc cgagccgcct
     1381 gctgagcgcg gcctcggccg attcctggac gcgcggatcg agcgtggtga agatgcgcag
     1441 gccctcggtg cgcagatcgg cctcgtcgta atcgcggcgc agctgccggt gcacgagctg
     1501 caggaacgcg gggtatggcg tcgtgcccat gccggtcttc ttgcttacgt tcagcggcat
     1561 cgcgcgcgcc tgcgcgtact cctcggcggt gatgcttttc tggtcccgcg cttcgcgcag
     1621 cacgagattg cgccgctcga gcgcccggtt cggatgccgg tacgggtcgt agtgcgccgg
     1681 gccccggatc agcccgacca ggagcgccga ctcggcgacg ctcagatgct ggacctcctt
     1741 gccgaagtag aactgcgcgg cgagcccgac gccgtggatg gcgcggtcgc ggtcctggcc
     1801 gaaatagatc tcgttcaggt aagtctcgag gatctcgtcc ttgtcgtaat gcagctcgag
     1861 cagcaccgcc atcgcgagct cggtcagctt gcgctggatc gtgcgctcgg gcgtgaggaa
     1921 gaagttcttg acgagctgct gcgtgatcgt gctgccgccc tgcacccggt cggtaaagag
     1981 gctgcgcgcc gcccgcgcga ggccgcgcac gtcgaacccg gcgtgcgcat agaacttgcg
     2041 gtcctcgacc gcgatcagcg tctggatgaa gtgcttcggc acgtcgggca ggcgcaccag
     2101 cacccggtct tcgttgcccg ccggatagat gcctccgatc gcgagcggct ccaggcgcgc
     2161 gagcggcagc tccgccccgc ccgcgtcgcg cagcgcagtg atcttgtcct tatcgaaaac
     2221 gacgcttatc cgcttggccg gctggcgccc gtcccagaac acgaatggac gcagcgccaa
     2281 ctccagcccg ttttctcttt tcgcatacca gcccgattcc ccttccggcg gagcttcgcg
     2341 gtagccgagc tggcgcagct cctccaccac atcggcctgc gcgatgcgca atcccgcgtg
     2401 cagctcgagc gggcgcgcgt aaatgcgcgc cggcagcgcg aagcgccggc cttcgaactc
     2461 ggtgcgcacc cggatgtcga ggtacagcgt aacgcccgcg gccagcagcg tgacggcgag
     2521 cagcgagcac cagagcaatc tgcgcaagga cagtctgcgg aaaagcgagc gccgcggcat
     2581 cgcggcattc tacttgcgcc ggtgctcgct ccgcgtgcga atgcgccggt ttgtcagtgg
     2641 tctgtcagat tcctgtcgtt attcgccgac cgtgtcggca gcgtatgccc gacgcgcgcc
     2701 catctgccgc tcctggccgc tcgtcgtgtc ctttctcgtc tggcgctcca tctcggcgtt
     2761 gatctcggcg ccgatcagga tcgcgtagga agagagcagg aaccacatga gcagtatcac
     2821 caccgcgccg gccgcgccgt aggtctcgtt gtagtttccg aagcgggtga catagagcga
     2881 gaacagcgcc gagccgacga tccacaacgc cacggcgatc gtagcgcccc agcttaccca
     2941 ccgccagcgc ggctgcgcgc ggctcgggcc gtagcggtag atgacggcga acgcgacgat
     3001 tgcgagcagc gcgaggatcg gccagcgcgc gagggacacc accgtctcga gcagcgagct
     3061 cagcccgatg aaattcagga cggccggcag gccgacaacc agcccgatcg ccaggatgac
     3121 agcgatgacg ccgccgacag tgaggatcag cgccgttccg taaaaccgga cgaggccgcg
     3181 tcgctcctct tcatcgtagg cgacgttgag cgcctccatc agcgtgcgca cggcggagga
     3241 ggcgctccat agcgcgacca gcaggccggc gatcgcgccc aggccgagcg cgccgcggtt
     3301 ggtggagacg aggtcgtgga gctgcgtcag cacgatcttt gccgcttccg gcgggaggat
     3361 gccgctcagg ggggcgacgt gccgctcgat ctgcgcgggg tccgcgaaca ggccgtacat
     3421 cgccactagc gcgacgaggc cgggaaatat ggcgagcaag gcgtagaacg ccacccccgc
     3481 cgcgatgatc gacagccggt cacgcttgat ctggtccttc actcgccgcg tgatgtcgag
     3541 ccagccgcgc gcagggatct cgcgcggcgt gctcgcctgg cggccgcggg actcggccgc
     3601 ggccgccact accaggcgcc ctttcggtcc gggcccgccg ccgtcggccc ggggtgctcg
     3661 ctctgcgcct gttgcaacgg cacgtttcct tccggctgtc ctggctggga ctccgctccc
     3721 ggccgggacc cctgtcccac ggtttccgca acctgcttta cggtctcctg agctctctcg
     3781 agctgttgcg aacccatttc tcttgccttc tcggcaagac cccggcttgc atcgcccatg
     3841 agcctttcct cgtaccgggt ccttggcgcc gccgcgccaa ggatcgcgcc gatggcgagc
     3901 ccgatcgcac caagcgcaag gggttgctct tgcacgaggt agtcgacgct gccgcgtgcc
     3961 cgctcccatt ggtaccgcgc agtgtcggta gcgtgggtcg cgcgctcgcg cagtgagctg
     4021 atatttccgg caatccggtc cttggtcgat gccagcgttt cagaagcgct ctgcatggtt
     4081 tggctcgtct tgtcgcgcac cgaagtcatg ccttctctga ccgaagtcat tccttcctgc
     4141 ag
//
LOCUS       DASUNT010000600         3892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_603325, whole genome shotgun sequence.
ACCESSION   DASUNT010000600 DASUNT010000000
VERSION     DASUNT010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603325"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..921
                     /locus_tag="VFX94_12170"
     CDS             55..921
                     /locus_tag="VFX94_12170"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX5768983.1"
                     /translation="MELIQALKAYPALLYGTSAFFGLCVGSFLNVVIHRLPKMLERGW
                     REECAELAGQPLPHEPAYSLVVPRSACPACGHRIRAWENVPILSWLVLGGKCSACGTG
                     ISLKYPLVEALAGLGAAYAAWRFGATVAALGAALFIWFTIALAFIDQQTGLLPDSLTL
                     PLVWIGLLANLVGAFVPLRDAVLGAVAGYLALWLIFHGYRLLTGKEGMGYGDFKMNAA
                     VGAFLGWKLLPLVILLSSLVGLFFGALQMFAKRGRWEAGFRFHFGPYLAIAAIVAMFW
                     GESILRWYLQRL"
     gene            918..1520
                     /gene="coaE"
                     /locus_tag="VFX94_12175"
     CDS             918..1520
                     /gene="coaE"
                     /locus_tag="VFX94_12175"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234741.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX5768984.1"
                     /translation="MTFVVGLTGGIGSGKSAAAEEFARLGAAIVDTDVIAHELTGKGG
                     AAIPAIEALFGHDVLGPDGSMDRRKVRERVFADPAAKQKLEALLHPMIREESARRIAA
                     AAGPYVVHVVPLLIESPDYRARVDRVLVVDSPEQTQIERVRARSGLSEREVRAILRNQ
                     VTRAARVAAADDVIDNGGSREALREQVAALHQKYLQFAGK"
     gene            1517..2272
                     /gene="zapD"
                     /locus_tag="VFX94_12180"
     CDS             1517..2272
                     /gene="zapD"
                     /locus_tag="VFX94_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEX5768985.1"
                     /translation="MITYEYPLNERIRTLLRLEDLFERSRHFIARTEPHDHHVALLTL
                     FEIMEVAGRADLKSDLLQELERQKQVLLSFRNNPDIAERALASVLRDIEQASQALFSM
                     TGKIGQYLRENDWLMSIKQRTGIPGGACEFDLPSYHFWLHRRSEERTGQLAAWTSPLY
                     PLRDGSAIVLRILRESGKPQRLAAPQGMFQQMLGGKTAQMLRLRLEDNMPCVPEISAN
                     KYVLNIRFLAQNAEEKARTADWDVGFELTFCNL"
     gene            2260..2442
                     /locus_tag="VFX94_12185"
     CDS             2260..2442
                     /locus_tag="VFX94_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEX5768986.1"
                     /translation="MQSVRIVRCPRCGTRTEYSAQNKWRPFCSERCRMIDLGRWAAEE
                     YRLPEEEDARSKPHES"
     gene            complement(2417..3319)
                     /locus_tag="VFX94_12190"
     CDS             complement(2417..3319)
                     /locus_tag="VFX94_12190"
                     /inference="COORDINATES: protein motif:HMM:NF006530.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HEX5768987.1"
                     /translation="MIEVAAAVIQRPDGAFLLAQRPPGKVYAGYWEFPGGKVEPGEPP
                     DRALARELHEELGIEIGPAYPWITRVYTYPHGTVRLRFFKVFSWKNEPHPREDQAIAW
                     QESGAAMAAPMLPANAPVLASLKLPVEYAVTNAAGYGSADMLARLERRLAQGLKLIQV
                     REPALSAEDRSLFTRQVIGLAHRHGCKVMVKSAFPGADGVHFPAVELMNLGTRPRGLA
                     AASCHTRAELEHAMKLELDFAVLGPVKEVKGKSPALGWARFAELARGASIPVYAIGGL
                     TRADIRQAWRAGAHGVAMIRGAWT"
     gene            complement(3329..>3892)
                     /locus_tag="VFX94_12195"
     CDS             complement(3329..>3892)
                     /locus_tag="VFX94_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5768988.1"
                     /translation="IVKGLLNKYARQGLRLIEVEKNDLVDLPTIVDQVAPRPERFLLF
                     CDDLSFHGAEEGYIALKVALDGSISTTSENLLIYATSNRRHLMPEYMAENLETKYLGD
                     EIHPGETVEEKISLSERFGLWVTFYPFDQDEYLEIVGQWLKSFDASFSEKTKEEALQW
                     ALQRGSRSGRVAYQFARDWAGRHARKR"
BASE COUNT          618 a         1314 c         1295 g          665 t
ORIGIN      
        1 cgcggaaagt aatttccgcg cccttttgtt tgggccgagg ccaagcgacg cgcgatggaa
       61 ctcatccagg cattgaaggc ataccccgcg ctgctctacg gcacgtcggc gttcttcggc
      121 ctgtgcgtgg gctcgttcct gaacgtggtc atccaccggc tgccgaagat gctggagcgc
      181 gggtggcgcg aagaatgcgc tgagctcgcc gggcagcccc tgccccacga gccggcgtat
      241 agcctcgtcg tgccgcgctc ggcgtgcccg gcctgcgggc accgcatccg cgcatgggag
      301 aacgtgccga tactgagctg gctcgtcctc ggcggcaaat gctccgcgtg cggaaccggt
      361 atttcgctca agtatccgct ggtggaagcg ctcgcgggtc taggggccgc gtacgccgcg
      421 tggcgcttcg gcgcgacggt cgcggcgctg ggcgctgcgc tgttcatctg gttcacgatc
      481 gcgctcgcgt tcatcgacca gcaaaccgga ttgctgcccg acagccttac gctcccgctc
      541 gtatggatcg ggctgctggc gaacctcgtc ggggcgttcg ttccgctgcg cgatgcggtc
      601 ctcggggcgg tcgccgggta cctcgcgctc tggctgatct tccacggcta ccgcctgctc
      661 accggcaagg aaggcatggg ttacggcgac ttcaagatga acgccgcggt gggcgcattt
      721 ctcggctgga agctcctgcc cctggtgatc ctgctctcct cgctcgtagg cctgttcttc
      781 ggcgcgctgc agatgttcgc caagcgcggc cgctgggaag cgggattccg cttccatttc
      841 ggcccgtatc ttgcgatcgc cgccatcgtg gcgatgttct ggggcgagtc gatcctgcgc
      901 tggtatttgc agcggctgtg acctttgtcg tcggcctcac cggaggaatc ggcagcggca
      961 agagcgcggc ggccgaggag ttcgcgcggc tcggggcggc gatcgtggat accgatgtca
     1021 tcgcacacga gctcaccggg aaaggcggcg cggcgatccc cgcgatcgag gcgctcttcg
     1081 gccacgacgt gctcggcccg gacggctcga tggatcgcag aaaggtccgc gaacgcgtct
     1141 ttgccgaccc ggccgcaaag cagaagctcg aagcgctgct gcacccgatg atccgcgagg
     1201 agtcggcccg gcgcatcgct gctgccgccg ggccctatgt ggtgcatgtc gtaccgctgc
     1261 tcatcgagtc gcccgattat cgcgcgcgcg tcgaccgggt gctggtggtg gactcccccg
     1321 agcagacaca gatcgagcgc gtgcgcgcgc gcagcgggct ttccgagcgc gaggtgcgcg
     1381 ccatcctgcg caaccaggtg acgcgcgccg cgcgtgtggc ggccgccgac gacgtgatcg
     1441 acaacggcgg ctcgcgcgaa gcgctgcgcg aacaggtcgc cgctctgcac caaaaatacc
     1501 tacaattcgc aggcaagtga tcacctacga atatccgctg aacgagcgca tccgcacgct
     1561 gctgcggctt gaggatttgt tcgaacgttc gcgccacttc atcgcgcgca ccgagccgca
     1621 cgaccaccac gtcgcgctgc tcacgctgtt cgagatcatg gaggtcgcgg gccgcgcgga
     1681 cctgaagtcg gacctgctgc aggagctgga gcgccagaag caggtgctgc tctcgttccg
     1741 caacaacccg gacattgccg agcgggcgct cgccagcgtg ctgcgcgaca tcgagcaggc
     1801 gtcgcaggcg ctgttctcga tgaccggcaa gatcggccag tacctgcgcg agaacgactg
     1861 gctgatgtcg atcaagcagc gcaccggcat cccaggcggc gcgtgcgagt tcgacctgcc
     1921 ctcctaccat ttctggctgc accgccgctc ggaggagcgc accggccagc tcgcggcctg
     1981 gacttcgccc ctctacccgc tgcgcgacgg ctcggccatc gtgctgagaa tcctgcgcga
     2041 gtcgggcaag ccgcagcgtc tcgccgcgcc ccagggcatg ttccagcaga tgctcggcgg
     2101 caagaccgcc cagatgctgc ggctgcgcct cgaagacaac atgccgtgcg tgcccgagat
     2161 cagcgccaac aagtacgtgc tcaacatccg cttcctcgcg cagaacgccg aagagaaggc
     2221 gcgcaccgcg gactgggacg tcggcttcga gctcacgttt tgcaatctgt aaggatcgtg
     2281 cgctgcccgc gctgcgggac gcgcaccgag tattcggcgc aaaacaagtg gcgcccgttc
     2341 tgcagcgagc gctgccgcat gatcgacctc ggcagatggg ccgcggagga ataccgcctg
     2401 cccgaagagg aagacgctag gtccaagccc cacgaatcat agccacgccg tgcgcgcctg
     2461 cgcgccaagc ctgccggatg tcggcccgcg tcaggccgcc gatcgcgtag accggaatgc
     2521 tcgcgccgcg ggcgagctcg gcgaagcgcg cccatccgag cgcgggcgac ttgcccttaa
     2581 cctccttcac cgggccgaga acggcgaagt cgagctcgag cttcatcgca tgctcgagct
     2641 ccgcgcgagt gtggcaggaa gccgcggcga ggccgcgggg ccgcgttccg aggttcatca
     2701 actccacggc cggaaagtgg acgccgtccg cgccgggaaa cgcggacttc accatgacct
     2761 tgcagccatg gcggtgcgcc aggccgatca cctgccgggt gaaaaggctc ctgtcctcgg
     2821 cactgagcgc gggctcgcgc acttgtatca gcttgagccc ctgcgcgagc cgcctttcca
     2881 ggcgcgcgag catgtcggcg ctgccgtatc cggcggcgtt cgtgactgca tattcgaccg
     2941 gcagcttgag cgaggcgagt accggcgcgt tcgccggcag catgggcgcg gccatcgccg
     3001 cgccggattc ctgccacgcg atcgcctggt cttcacgcgg atggggctcg ttcttccagg
     3061 aaaaaacctt gaaaaaccgc agccgcaccg tgccatgcgg gtaggtgtat acccgcgtga
     3121 tccagggata tgccggcccg atctcgatgc cgagctcctc gtgcagctcg cgggcgaggg
     3181 ctcggtcggg cggctcgccg ggctccacct tgccgccggg aaattcccag tagccggcgt
     3241 agaccttccc cggcgggcgt tgcgcgagca ggaacgcgcc gtccgggcgc tggatcaccg
     3301 ccgccgcgac ctcgatcacc gggctgttct acctcttccg ggcgtgccgg ccggcccagt
     3361 cccgggcgaa ctgataggcg acgcggccgc tgcgcgagcc gcgctgcaat gcccattgca
     3421 gcgcctcctc cttcgtcttc tcactgaaag aggcatcgaa cgatttcagc cactggccga
     3481 cgatctcgag gtattcatcc tggtcgaagg gatagaacgt cacccacagg ccgaagcgct
     3541 cggaaagcga gatcttctcc tccaccgtct cgcccggatg gatctcgtcg ccgagatact
     3601 tcgtctcgag gttctccgcc atgtattcgg gcatgaggtg gcggcggttg gaggtggcgt
     3661 agatgaggag gttctcggag gtcgtggaga tcgagccgtc gagcgccacc ttgagcgcga
     3721 tgtagccctc ttcggcaccg tgaaagctca gatcgtcgca aaagaggagg aagcgctccg
     3781 ggcgcggcgc cacctggtcc acgatggtcg gcaggtcgac gaggtcgttc ttctccacct
     3841 cgatcagtcg caggccctgc ctcgcgtact tgttgagcag ccctttcacg at
//
LOCUS       DASUNT010000601         2904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_953599, whole genome shotgun sequence.
ACCESSION   DASUNT010000601 DASUNT010000000
VERSION     DASUNT010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953599"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..872)
                     /locus_tag="VFX94_12200"
     CDS             complement(63..872)
                     /locus_tag="VFX94_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEX5768989.1"
                     /translation="MRIYGVDFTCAPRKAKPITVASGVLQGNLLQLEALEKLASFAAF
                     ESFLARRGPWIGGFDFPFSLPRELVRDLGWPSHWPALVAHCAAMDRLAFRASLERYRA
                     SRRAGSKYVHRATDYPAGSSSPMKLVNPPVALMFHEGARRLAACDVHMPALRDGPPSK
                     VALEAYPGLLVRKQLGIRASYKSDTRREHTAARAAVRKAILRALRGGEPLGIGLQLPR
                     RLENEIAADGPGDLLDAVICAAQAAWGWRRRSANFGLPVHAPAGEGWIVTA"
     gene            complement(869..1720)
                     /locus_tag="VFX94_12205"
     CDS             complement(869..1720)
                     /locus_tag="VFX94_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX5768990.1"
                     /translation="MDLVELQIFKTIAEQGGITRAAAALHRVQSNVTTRVKQLEERLG
                     TKLFHRQGRKLVLSAEGRLLLEYAERLLRLSTEAQAAIRSQVLHGQFRLGALESTAAA
                     RLPPVLAAYHRAHPGVRLELFTGTSGALLDKVHRGDIEAAFVAEPFAAKNLEMQHVFT
                     EELVLITPRSHGRVSSPKQLEQRVVLAFPAGCSYRRRLESWLGRSSIVAERVMEYGSY
                     HAITACVAAGGGIAVVPRSVLRVVGAESQLGVHALPPQVAKARTMLVWPRGRDSAALA
                     ALREELG"
     gene            1813..2790
                     /locus_tag="VFX94_12210"
     CDS             1813..2790
                     /locus_tag="VFX94_12210"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX5768991.1"
                     /translation="MAIRTALTEALGIRHPILLAPMDLVSDARLTAAVSAAGALGLLG
                     GGYGDEPWLRRELELLERSKARFGVGFITWSMARQPKLLDIALERKPVAVMLSFGDPA
                     PFAERIKRAGARLICQVQTLAMAKEAATSGADILIAQGAEGGGHGASRGLVSLVPEIV
                     DAVGARVPVVASGGLADGRGLAAGLMLGASGAMFGTRFYASHEASGAHAAKERIRAAG
                     GDDSLRSIVFDISRRNVWPAPFTGRCLRNPFIDRWYGREVELLRSADGEAERLAEARR
                     KQDFDVAPVIAGEACGLVHDILPAREIVERIMHEAERLLARGARQPEAA"
     gene            2756..>2904
                     /locus_tag="VFX94_12215"
     CDS             2756..>2904
                     /locus_tag="VFX94_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEX5768992.1"
                     /translation="MRAALDSRKPRDSAAGVAAAGLIALAVAMGIGRFAFTPLLPMMQ
                     VDAGLS"
BASE COUNT          450 a          992 c         1008 g          454 t
ORIGIN      
        1 ggaggaagac cgcctcgcac atttctgcat ggaatccaaa ggctacaagc tcgtgccggt
       61 gcctaagcag taacgatcca tccttcgccg gcgggcgcgt gcaccggtag cccgaagttc
      121 gcgctgcgcc gcctccatcc ccatgccgcc tgcgccgcgc agatcacggc gtcgagcaga
      181 tcgcccgggc cgtcggcggc gatttcgttt tccagtcggc gtggcagctg aagaccgatt
      241 cccaacggct cacctccgcg caacgcccgc aggattgctt ttctcacggc ggcgcgggcc
      301 gcggtgtgct cgcgtcgcgt gtcgctcttg taggaagcac gaatgccgag ctgcttgcgt
      361 acgagcaggc cggggtacgc ttccagcgcg actttcgaag gcggaccgtc tcgcaaggca
      421 ggcatgtgca catcgcaggc ggcgagccgg cgggcgcctt cgtggaacat gagcgcgacc
      481 ggaggattca cgagcttcat gggactcgaa gagccggccg ggtagtcggt agcgcgatgc
      541 acgtacttgc tgcccgcacg cctcgatgcc ctgtagcgct cgaggctcgc gcgaaaggcg
      601 aggcggtcca tggccgcgca atgcgccacc agcgccggcc agtgcgaggg ccagccgagg
      661 tcgcgcacca gctcgcgcgg caggctgaac ggaaagtcga agccgccgat ccacggcccg
      721 cgccgcgcaa ggaagctctc aaaggcggcg aagctcgcta gcttttccaa cgcctctagc
      781 tgcagcaggt tgccctggag aacccctgaa gcgacggtga tcggcttcgc cttgcgcgga
      841 gcgcaggtga agtcgacgcc gtagatcctc aaccgagctc ttcgcgcaac gcggcaagcg
      901 cggcggaatc gcggccgcgc ggccagacga gcatcgtgcg cgccttggcg acttgcggcg
      961 gcagcgcatg cacgccgagc tggctttccg cgccgaccac ccgcagcacc gagcggggaa
     1021 cgaccgcgat gccgccgccg gcggccacgc acgccgtgat cgcgtggtac gagccgtact
     1081 ccatgacacg ctcggccacg atgctggagc ggccgagcca cgactcgagc cgccggcgat
     1141 acgagcaccc ggcagggaag gcgagcacta cacgctgctc gagctgcttg gggctgctca
     1201 cgcggccgtg gctgcgtggc gtgatgagga cgagctcctc cgtgaagacg tgctgcatct
     1261 ccagattctt cgccgcgaat ggctcggcca cgaatgccgc ctcgatgtcg ccgcggtgca
     1321 ccttgtcgag cagcgcgccg ctcgttccgg tgaagagctc gagccgcacg ccgggatgcg
     1381 cgcgatgata ggcggcgagc accgggggca ggcgcgccgc ggcagtgctt tcgagcgcgc
     1441 cgagccggaa ctggccgtgc agcacctggc ttcgaatcgc ggcctgcgcc tcggtcgaca
     1501 ggcgaagcag ccgctcggcg tattcgagga gcagccggcc ttcggcagaa agcacgagct
     1561 tgcgtccctg gcgatggaag agcttcgtgc cgaggcgctc ctcgagctgc ttcaccctcg
     1621 tggtgacgtt cgattgcacc cggtgcagcg cggctgcggc gcgcgtaatg ccgccctgct
     1681 cggcgatggt cttgaagatc tgcagctcga ccaggtccat cgttcagaaa tggagaatca
     1741 tccgttcaga attattcatt tatcaagaaa gatgcgtcaa gctagagttc gctctctccg
     1801 gcgaggtgtc ccatggcgat ccgcactgcg ttgaccgaag ctctaggaat ccggcacccc
     1861 atcctgctcg cgccgatgga cctcgtgtcc gatgcgcgcc tcaccgctgc ggtaagcgcc
     1921 gccggcgcgc tcgggctgct cggcggcggc tacggcgacg agccatggct gcgccgcgag
     1981 ctcgagctcc tcgagcgctc gaaggcacgc ttcggcgtcg ggttcatcac ctggagcatg
     2041 gcgcggcagc cgaagctgct cgacatcgcg ctcgagcgca agccggtcgc ggtcatgctt
     2101 tcgttcggcg atccggcccc ctttgccgag cgtatcaagc gagccggcgc gcgcctcatt
     2161 tgccaggtgc agacgctcgc catggccaag gaggcggcca ccagcggcgc cgatatcctg
     2221 atagcgcaag gcgcggaggg cggcggccac ggcgcctcgc gcgggctggt tagcctcgtc
     2281 ccggagatcg tcgacgcggt cggcgcgcgg gtcccggtgg tcgcgtccgg cggccttgcc
     2341 gacgggcgcg gccttgcagc cggcctcatg ctgggcgcct cgggcgcgat gttcggcacg
     2401 cgtttctacg cctctcacga agcgtcgggg gcgcacgccg ccaaggagcg catccgggcc
     2461 gcaggcggag acgactcgct gcgcagcatc gtgtttgata tctcgcgccg caacgtctgg
     2521 cctgcgccgt tcaccggacg ttgcctgcgc aacccgttca tcgatcgctg gtacggccgc
     2581 gaagtcgagc tcctgcgaag cgccgacggc gaggccgagc gcctggccga ggcgcgccgc
     2641 aagcaggact tcgacgtcgc gccggtgatc gccggcgagg cttgcggcct ggtgcacgac
     2701 atcctgccgg cgcgggagat cgtggaacgc atcatgcacg aagcggaacg actccttgcg
     2761 cgcggcgctc gacagccgga agccgcgtga ttctgccgcg ggcgttgccg cggccggctt
     2821 gatcgcgctc gccgtggcga tgggcatcgg ccggttcgcg ttcacgccgc tgctgccgat
     2881 gatgcaggtc gacgccggcc tgtc
//
LOCUS       DASUNT010000602         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_779209, whole genome shotgun sequence.
ACCESSION   DASUNT010000602 DASUNT010000000
VERSION     DASUNT010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779209"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFX94_12220"
     CDS             complement(<1..212)
                     /locus_tag="VFX94_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768993.1"
                     /translation="MITHGHVDKIRHALAGMEDVLAQSHGGRDDEDALLEFRRMCWAA
                     LLLMDDSEAQQLIDQLVQRAKDLYSEG"
     gene            complement(596..1669)
                     /locus_tag="VFX94_12225"
     CDS             complement(596..1669)
                     /locus_tag="VFX94_12225"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX5768994.1"
                     /translation="MRRGAGLPARQARPARCLAAAHRPAISTLREKVAALARRLTRRA
                     ALPAPGRFSVSRKSSLHGFINVAPAPPWREYLLYLPRAFDALERAALVVWIHGCRQDP
                     EQFAAGTRIARFADERGFVVLLPRQSRMANSERCWNWFDRRTAGGAGEAAIVAAQATE
                     VRDKFRLDPRRVYVAGLSSGGTLAATLALRAPGVFAAAAIHSSLACGAATDAHDAARA
                     MLEGPRAATDAIALEARAKAADKARVPALVMHGAADTSVAPVNSVFLVRQFLLFNGLA
                     PEDLPAGAALPPAQVRPFRARVSDYLAADYHAGRRLAARLVTIPGVGHAWSGGDSKFE
                     FFDGGGLDATALICDFFASHSRK"
     gene            complement(1615..>2445)
                     /locus_tag="VFX94_12230"
     CDS             complement(1615..>2445)
                     /locus_tag="VFX94_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5768995.1"
                     /translation="QKPINLRQLRDVLTARRDGKRNAASIGTESESLRDVQSVRRAIA
                     NDELIVHYQPQYHIKTRALAGYEALVRWQHPVRGLLPAAAFIEAFETAGVIDQLTWIV
                     LKRVLADRRKWSSQATRVPVSVNMSALLLRDLALPERLYSLITEHGGNPSDIVLEITE
                     SGLMRELHTSLDILARIRLKQLHLSIDDFGTGYAMMHQLRRVPARELKLDMAFVQSMH
                     ADESADIILRKTLELAHDLDMSVVAEGVETPRQLDRLAEYGCDVAQGYLLGRPGPLVA
                     "
BASE COUNT          421 a          778 c          847 g          399 t
ORIGIN      
        1 ccctcggaat acaggtcctt cgcgcgctgc acgagctggt cgatgagctg ctgggcttcg
       61 ctgtcgtcca tgaggagaag cgctgcccag cacatgcgtc ggaactcgag aagggcgtct
      121 tcgtcatcgc ggccaccgtg cgattgcgcc agcacgtctt ccataccggc gagcgcgtgg
      181 cggatcttgt cgacgtgccc gtgcgtgatc acgggttaca tgtttccacc gacccggtga
      241 cctgcacaac ccaccatcac agctttttgc ggcgcagacc gtgactaaat tcacccctga
      301 ggctttctgg caacggcaac cctcgggtcg tcgccgtcag gcgacaaggc ggcggtttac
      361 gccggtaccg agatacgggt cccgacgggt gtcgatcggc tcgggactgc tttcgtcctg
      421 gtgagccagg gccagatgaa ctggggaata taggcaagca ctgccgacgc tttccccgac
      481 tggagccaag agtggtcggt gaagacgagc gtgagcggga gcgcgaccca cgtgccggcc
      541 tcaagaagat tgctgaactg tcaggcgagt agcgccaggc actagcccgg aagggctact
      601 tgcgagaatg cgaagcgaag aaatcgcaga taagggctgt agcatccagg ccaccgccgt
      661 cgaaaaactc gaacttggag tcaccgccgc tccaggcgtg gccgacgccc gggatggtga
      721 cgagccgggc ggcgaggcgg cggccggcgt ggtaatcggc ggcgaggtaa tcggagacgc
      781 gcgcccggaa gggacgtact tgcgcaggag gcaaggcagc gccagcgggc aggtcttccg
      841 gcgccaggcc gttgaagagc aggaactggc gcacgaggaa cacggagttc actggcgcca
      901 cgctcgtatc cgcggcaccg tgcatgacga gcgcaggcac gcgggccttg tcggccgcct
      961 tcgcgcgcgc ttcgagtgcg atcgcgtcgg ttgcggcgcg cggtccttcg agcatggcgc
     1021 gggctgcatc atgtgcatcc gtggcggcgc cgcacgccaa gctggaatga atcgctgctg
     1081 ctgcaaaaac gcccggcgcg cgcagtgcaa gcgtagcggc gagcgtcccg ccggaagaca
     1141 agccggccac atacacgcgg cgcgggtcga ggcggaactt gtcccttacc tcggtggcct
     1201 gtgccgcgac gatggccgcc tcgccggctc cccccgcggt gcgccgatcg aaccagttcc
     1261 agcagcgctc ggaattcgcc atgcggctct ggcggggaag caagacgacg aatccgcgct
     1321 cgtcggcgaa gcgtgcgatg cgcgtgccgg cggcgaattg ctccgggtcc tggcggcagc
     1381 cgtggatcca caccacgagc gcggcgcgct cgagagcgtc aaacgcgcgc ggcaggtaga
     1441 gcaggtattc gcgccatggc ggcgcgggcg caacattgat gaacccgtgc aggctcgact
     1501 tgcggctgac cgaaaagcgc ccgggcgcgg gcagcgccgc ccggcgcgtg agccggcgcg
     1561 ccagcgcggc gactttttct cttaacgtgc taattgccgg gcggtgggcg gcggctaggc
     1621 aacgagcggg ccgggcctgc cgagcaggta accctgcgcc acgtcgcagc cgtactcggc
     1681 gagccggtcg agttgccgcg gtgtctccac cccttcggcc accaccgaca tgtcgagatc
     1741 atgcgcaagc tcgagcgttt tcctcaaaat gatgtcggcc gactcgtctg catgcatcga
     1801 ctgcacgaag gccatgtcga gcttgagctc ccgcgccggc acgcgccgca actggtgcat
     1861 catcgcatag ccggtgccga aatcatcgat cgagaggtgc agctgcttca ggcggatgcg
     1921 cgccaaaatg tcgagcgaag tgtgcaactc gcgcatgagg ccgctttcgg tgatctccag
     1981 cacgatatcc gatgggttac cgccgtgctc ggtgatgagc gagtacagcc gctcggggag
     2041 cgcgaggtcg cgcaagagca gggccgacat gttaaccgac acagggacgc gcgtggcttg
     2101 cgagctccat ttcctgcgat cggcgagcac ccgcttgagc acgatccagg tcagctggtc
     2161 aatgacgccc gctgtttcga aagcctcgat gaaggccgcg gccggcagca gccccctgac
     2221 cgggtgctgc cagcgcacca gggcttcgta gccggcgagc gcgcgcgtct tgatatggta
     2281 ttgcggctgg tagtggacga tgagctcgtc gttcgcgatc gcgcggcgca cgctttgcac
     2341 gtcgcgcagg ctttcgctct cggtcccgat gcttgcggcg ttgcgcttcc cgtcgcgccg
     2401 cgccgttagg acgtcgcgca gctggcgtag gttgatcggc ttctg
//
LOCUS       DASUNT010000603         2268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_691735, whole genome shotgun sequence.
ACCESSION   DASUNT010000603 DASUNT010000000
VERSION     DASUNT010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691735"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..1062
                     /locus_tag="VFX94_12235"
     CDS             325..1062
                     /locus_tag="VFX94_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768996.1"
                     /translation="MSALHSFAEGGFAFLEGVFAYSQGAVALDGYAIERACFARPLPV
                     AEGFRRIAAHLQGLGRPKTALCACELRSPRPFSFAGFGEFNRGYVAVMEQWGLFRDRQ
                     NAVARSNVAPEINPPAEPSFYAFSYTVPVAHARRNFVVAGSGEWPEGGRFPEDIIARG
                     DVTEAGTRNKAAWVLDMMQARLTGLGGFGFGEASAVQVYTVRRFPIELVAQRAGSAVT
                     WHYCRPPIEGLEFEMDVRCVSLERRLG"
     gene            complement(1037..2032)
                     /locus_tag="VFX94_12240"
     CDS             complement(1037..2032)
                     /locus_tag="VFX94_12240"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5768997.1"
                     /translation="MKPLAWAGVALLASVPLTGIGNYPLHLIIMCLLWAYVYTSWSIM
                     GRLGLVSLGHGAFLGIGAYAVVMLWNHFSLSPWLGAPVAVVTSLAVAFMIGLPCFRFR
                     IVGHYFALVTLALSEVTRLVIVALRDHTGGSLGATPNTALAQGNTWSLAALQFSDKRV
                     WFYVILACWLAGLWVWARLDRSMTRLALEAISQEEDAAASVGINVTRSKLFVTLISAG
                     MTSLGGVLYAQYQVYINPETVSGIGISLQMVFGAIAGGMFVMLGPTVGAAFTLLLQEG
                     LRLALGDAFKQFPALDLAIYGLMLVLFIIYMPKGILGAALEWRARRRATPAAAPG"
     gene            complement(2029..>2268)
                     /locus_tag="VFX94_12245"
     CDS             complement(2029..>2268)
                     /locus_tag="VFX94_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5768998.1"
                     /translation="VLQYDIHPAIGLSFGPITFLICVLGGLGNLLGGLVAAFIFAQFI
                     SVGGFFFHLEWGYVIAFAFFIAVMFWRPQGILAGK"
BASE COUNT          449 a          703 c          769 g          347 t
ORIGIN      
        1 acaaaacgat gctcccttaa aggttcatca gccgttgttt ttcagcgagc acgcgcgccg
       61 cgtaccgtta cgtcatgtca ccagcaacgt aacgacttgt ccgtcaagga atgcaagccg
      121 cggcagacat cagtattgga cggaagttcg agagtgtgat gtaagggaac gctgatccaa
      181 cgtagactcc gagacgctca tactgggcgc gcggtagggg aagttcattt cattcgctag
      241 gcggacaaga aacgatgggg aacgcatgta gagtgaagcg ggcgaagcgt tatttcctcc
      301 tcctccacca acacaagctt ctcgatgagc gcgctccata gctttgccga aggcggtttc
      361 gcgttcctcg aaggggtatt cgcttactcc cagggcgccg tcgcgcttga tggctacgcc
      421 atcgagcgtg cgtgcttcgc gcggccgttg ccggttgccg aaggcttccg ccgcatcgcc
      481 gcgcaccttc agggcttggg acggccgaag acggcgctct gcgcctgcga gttgcgctcg
      541 cccagaccct tctcgttcgc cgggtttggc gagttcaacc gcggctatgt cgcagtgatg
      601 gagcaatggg gcctctttcg cgaccggcaa aacgcggtag cgcgctcgaa cgtagcgccc
      661 gagatcaatc cgcccgccga gccctcgttc tacgccttca gctacacggt ccccgtggcg
      721 cacgcgaggc gcaacttcgt ggtcgccggc agtggcgagt ggcccgaagg tgggcgcttt
      781 cccgaggaca tcattgcgcg cggcgacgtc accgaggcgg gcactcgcaa caaagccgct
      841 tgggtgctgg acatgatgca ggcgcgcctt acagggctcg gcggcttcgg cttcggcgag
      901 gcgagcgcgg tgcaggtgta caccgtgcga cgcttcccga tagaactcgt cgcgcaacgc
      961 gccggcagcg ccgtcacgtg gcactactgc cggccgccga tcgaggggct ggagttcgag
     1021 atggacgtgc gctgcgtcag cctggagcgg cggctggggt agcgcggcgg cgtgcgcgcc
     1081 actcgagcgc ggcaccgagt atgcccttcg gcatgtagat gatgaagagc accaacatga
     1141 gcccgtagat ggcgaggtcg agcgccggga actgcttgaa ggcgtcgccg agcgcaaggc
     1201 gcaggccctc ctgcaggagc agcgtgaacg ccgcgccgac cgtggggccc agcatgacga
     1261 acatgccgcc ggcgatcgcg ccgaagacca tctgcagcga gatgccgatg cccgacacgg
     1321 tctcgggatt gatgtagacc tggtactggg catagagcac gccgccgaga cttgtcatgc
     1381 cggcggagat gagcgtcacg aagagcttcg agcgcgtgac gttgatgccg accgacgccg
     1441 cggcgtcttc ctcctggctg atggcttcga gcgcgaggcg cgtcatgctg cggtccagac
     1501 gcgcccagac ccacagcccg gccagccagc atgccaggat gacatagaac cacacgcgct
     1561 tgtccgagaa ctgcagcgcg gcgagcgacc aggtgttgcc ctgcgcgagc gcggtgttcg
     1621 gcgtcgcgcc gagcgaaccc ccggtatggt cgcgcagcgc cacgatcacg aggcgggtca
     1681 cctcggagag cgcgagagtc accagcgcga agtagtggcc gacgatgcgg aagcggaagc
     1741 agggcaggcc gatcatgaag gcgaccgcga ggctggtgac cacggccacg ggtgcgccga
     1801 gccatggcga caggctgaag tggttccaca gcatcaccac cgcgtaggcc ccgataccga
     1861 ggaaggcgcc atggccgagg ctcaccaagc ccaggcgccc catgatcgac cagctcgtgt
     1921 acacgtacgc ccagagcagg cacatgatga tgaggtgcaa cggatagttg ccgataccgg
     1981 tgagcggtac ggaggccagc aacgcgaccc cggcccaggc gagtggcttc acttgccggc
     2041 gaggatgccc tggggccgcc agaacatcac cgcaatgaag aaagcgaagg cgatcacgta
     2101 gccccactcg aggtggaaga agaacccgcc caccgagatg aactgcgcga agatgaaggc
     2161 ggcgaccagg ccaccaagca gattgcccag gccgccgagc acgcagatga ggaaggtgat
     2221 cgggccgaag gagaggccga ttgccggatg gatgtcatat tgcagcac
//
LOCUS       DASUNT010000604         2467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_429943, whole genome shotgun sequence.
ACCESSION   DASUNT010000604 DASUNT010000000
VERSION     DASUNT010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429943"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="VFX94_12250"
     CDS             <1..947
                     /locus_tag="VFX94_12250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX5768999.1"
                     /translation="HSNAVRSRARANAEAHYALPPEFYKFWLDDPLMLYTCAYWTDGT
                     RTLEEAQRNKCDHVARKLLLKPGEEVIDVGSGFGGFMFHAHERYGVKVTAVNTTGSQA
                     EHARREIERKRLGNALSMQMHEDFRDAGQRQFDKVASIGCLEHAGRDQLAELIRAHAG
                     YLKKGGIGLIHFIGHVGRFDTDFFIRKYVFPGGWIPSLADVIVEMENAGLEILDVENL
                     RRHYALTLDVWAERFDRSWDKIRALDPIRFDERFRRIWRVYLYGCAEMFRVPNGRTHL
                     FQVVFSKGNVTSSSYPMTREFLYEQVKAEAAPRRVGHG"
     gene            940..2280
                     /locus_tag="VFX94_12255"
     CDS             940..2280
                     /locus_tag="VFX94_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5769000.1"
                     /translation="MADAAAAHAAKRESLRESLRQGAGPVALGKETSNLFRDREARER
                     RRLDVRGFTQVLRISDGWVEAEGMIPYDDLTRECLARAVMPAVVPQLKTITLGGAVAG
                     VGIEASSHRQGLVHDTMLELDVLLGDGRVVSCTPDNAHADLFFGFPNSYGTLGYALRV
                     KARTVPVKPYVHLSHLPFKNGRDYFSQLEEHLRAGKADFVDGTIFSPERLFITLGRFV
                     DSAPFTSDYTYKNIYYRSIAEKREDYLTAHDYIWRWDTDWFWCSKNVFAQNAFIRRFV
                     YGKNRLGSRTYTKIMRWNSRVGATKKLERILGLHSESVIQDVDIPISRAAEFLDFYSQ
                     EIGLWPQWICPIGPQSHSGRFVLYPVKNTWYVNFGFWDLKRTREAHAPGHFNRMIEDK
                     VSELGGIKSLYSDSFFSEADFHARYGGAAYDALKAKYDPVGAFPRLYDKCVLRA"
BASE COUNT          429 a          830 c          782 g          426 t
ORIGIN      
        1 cgcattcgaa cgccgtgcgc tcgcgggcgc gcgccaacgc cgaggcgcac tacgcgctgc
       61 cgccggagtt ctacaagttc tggctcgacg acccgctcat gctctacacc tgcgcgtact
      121 ggaccgacgg cacgcgcacg ctcgaggagg cgcagcgcaa caagtgcgac cacgtggcgc
      181 gcaagctgct cctcaagccg ggcgaggagg tgatcgacgt cggcagcggc ttcggcggct
      241 tcatgttcca cgctcacgag cgctacggcg tgaaggtgac cgcggtcaac accaccgggt
      301 cgcaggccga gcacgcgcgc cgcgagatcg agcgcaagcg cctcggcaac gcgctctcca
      361 tgcagatgca cgaggacttc cgcgacgccg ggcagcgaca gttcgacaag gtggcctcga
      421 tcggctgcct ggagcacgct gggcgcgatc agcttgcgga gctcattcgc gcgcacgcgg
      481 gctacctcaa gaagggcggc atcggcctca tccacttcat cggtcatgtc gggcgcttcg
      541 acaccgactt cttcatccgc aagtacgtgt tccccggggg ctggattccg agcctcgccg
      601 atgtgatcgt cgagatggag aacgccggcc tggaaattct cgacgtcgag aacctgcgcc
      661 gccactacgc tctcacgctc gacgtgtggg cggagcgctt cgaccgcagc tgggacaaga
      721 tccgcgcgct cgacccgata cgcttcgacg agcgcttccg gcgcatctgg cgcgtgtacc
      781 tctacggctg cgccgagatg ttccgcgtgc cgaacggccg cacgcacctg ttccaggtgg
      841 tgttctccaa gggaaacgtc accagctcga gctacccgat gacgcgcgag ttcctgtacg
      901 agcaggtgaa ggccgaagcc gccccgcgcc gcgtcgggca tggctgacgc ggctgcggcg
      961 catgccgcca agcgcgaatc tctccgagag tccctgaggc agggcgccgg gccggtcgcc
     1021 ctggggaagg agacatccaa ccttttccgc gaccgcgagg cccgcgagcg gcgccggctg
     1081 gatgtccggg gctttactca ggtcctgcga atctccgacg gttgggtcga agccgaaggc
     1141 atgattccct acgacgacct gacccgcgaa tgcctcgcgc gcgccgtgat gcccgccgtg
     1201 gtgccgcagc tcaagaccat cacgctcggc ggcgcggttg cgggggtcgg catcgaggct
     1261 tcgtcgcacc gccaaggcct cgtgcacgac actatgctcg agctcgacgt gctgctcggc
     1321 gacggccgcg tcgtgagctg cacgccggac aacgcgcacg ccgacctctt ctttggcttt
     1381 cccaactcgt acggcacgct tggctatgcg ctgcgcgtga aggcgaggac cgtgccggtg
     1441 aaaccgtatg tgcacctgtc gcacttgccg ttcaagaatg gacgagatta tttctcgcaa
     1501 ctggaagagc atctgcgcgc gggaaaggcc gatttcgtcg atggcacgat cttttcgccc
     1561 gagcggcttt tcatcacgct cggccgtttt gtcgattcgg cgccgtttac cagcgattac
     1621 acgtacaaga acatctacta ccgctcgatc gccgagaagc gcgaggacta cctcaccgcg
     1681 cacgattaca tctggcgctg ggacaccgac tggttctggt gctcgaagaa cgttttcgcg
     1741 cagaacgctt tcatcaggcg attcgtctac ggcaagaacc ggctgggctc gcgcacctac
     1801 acgaagatca tgcgctggaa cagccgcgtc ggcgcgacga agaagctcga gcgcatcctc
     1861 gggctgcatt cggaatcggt gatccaggac gtcgacattc cgatctcgcg cgcggccgag
     1921 ttcctcgact tctactcgca ggagatcggc ttgtggccgc agtggatctg tccgatcggg
     1981 cctcaatccc actccggccg attcgtgctc taccccgtga agaacacctg gtacgtcaac
     2041 ttcggcttct gggacctgaa gcgcacgcgc gaggcgcacg cgccggggca cttcaaccgc
     2101 atgatcgagg acaaggtgag cgagctcggc ggcatcaagt cgctgtactc cgacagcttt
     2161 ttctccgagg ccgatttcca cgcccgctac ggtggcgccg cctacgacgc gctcaaggcg
     2221 aaatacgacc cggtgggagc gttccctcgt ctctacgaca aatgcgtctt acgggcatga
     2281 attcccacag gctcgaagtg ctcggcctgg caaacgtctt ctcgtggacg cactggcagt
     2341 cgacgcagcg caccgcggcc gggttggcgc gcagccgctc gacgccgatg tcgggcgccg
     2401 caccggcgag gtcctcgaag cggtcgcggc gtacccgctc gacgtcgtca cgcagctcgg
     2461 ctatcag
//
LOCUS       DASUNT010000605         3106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1130079, whole genome shotgun sequence.
ACCESSION   DASUNT010000605 DASUNT010000000
VERSION     DASUNT010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130079"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VFX94_12260"
     CDS             <1..472
                     /locus_tag="VFX94_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5769001.1"
                     /translation="PHRTPRLEPLGVHEVLERVRSLVRAEFGAEIVRDYDPSLPEVMG
                     DREQLIQAVLNVARNAAQAGSKTVIFRTRVLRQVTILKRRCKLALELQVIDDGPGVAD
                     EIQDRIFHPLVSGREGGSGLGLSLAQTFVHYHHGVVEFESQPGRTIFRILLPIR"
     gene            469..1905
                     /gene="ntrC"
                     /locus_tag="VFX94_12265"
     CDS             469..1905
                     /gene="ntrC"
                     /locus_tag="VFX94_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEX5769002.1"
                     /translation="MKPVWIVDDDRSIRWVIEKALSREGIEFNSFSSAQEALDALSGA
                     TPEVLVSDIRMPGRSGLELLQAVKQRHPAVPVIVMTAYSDLDSAVAAFQGGAYEYLPK
                     PFDIDQAVELIRRALEERRRDSHEAEGAVENKEILGQAPAMQEVFRAIGRLSQSSATV
                     LITGESGTGKELVARALHRHSARAQQPFIAINTAAMPKDLLESELFGHERGSFTGAQQ
                     QRRGRFEQAEGGTLFLDEIGDMPAELQTRLLRVLSDGTFYRVGGHQQIKVNVRVIAAT
                     HHNLEQRVREGSFREDLYHRLNVIRLRLPSLRERAEDIPLLAKHFLARSAQQLGVEPK
                     RIAEEALTHIARLDFPGNVRQLENLCHWLTVMAPGQVIDLGNLPPEFRRELPGDGPAG
                     AADWIAALEQEAERRLARGDTGILDALGRQFERALISKALARTGGRRIDAANLLGMGR
                     NTITRKIQELGIDGDKSGNSGTHSGTEP"
     gene            complement(1950..3005)
                     /locus_tag="VFX94_12270"
     CDS             complement(1950..3005)
                     /locus_tag="VFX94_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769003.1"
                     /translation="MKPYQILILGASYGSLLATKLLLAGHSVKLVCLPAEADAINTDG
                     TRVRLPVKGREGLVEVHSKKLPGRLSADGPQTIKPSEYDLVALAMQEPQYRSAGVREL
                     LDAIAKAKVPCMSIMNMPPLPYLARIPGVSTKELRSCYTDASVWDSFDPALMTLCSPD
                     PQAFRPPEEKVNVLQVRLPTNFKSACFPSEANTAILKKLEADIEAARFEKMELPVKLK
                     VHESVFVPLAKWAMLIAGNYRCVTREGPRSIKDAVHSDVNATRAAYNWVVNLCLLLGA
                     KETDLVPFDKYAAAAQSLGSPSSAARALYGGAPYIERVDRLVQAIAAQKGLRLPALDE
                     VVGLVDAKLEANRKKAA"
BASE COUNT          549 a         1005 c         1016 g          536 t
ORIGIN      
        1 gccgcaccgc accccgcgtc tcgagcccct gggcgtgcac gaggtgctcg agcgggtgcg
       61 gagcctggtg cgcgccgagt tcggagccga gatcgtgcgc gactacgatc cgagcctgcc
      121 cgaggtcatg ggtgatcgcg agcagctcat ccaggccgtg ctcaatgtcg cgcgcaacgc
      181 cgcacaagcc ggcagcaaga ccgtcatatt caggacgcgc gtgctgcgcc aggtgaccat
      241 cctgaagcgc cgctgcaagc tggcattaga attgcaagtc atcgacgacg gcccgggcgt
      301 ggcggacgag atccaggaca ggatcttcca tccgctggtt tcgggaaggg aaggcggctc
      361 gggcctcgga ctctcgctcg cgcagacgtt cgtgcactac caccacggcg tcgtcgagtt
      421 cgagtcgcag ccgggccgga ccatattcag gatcctgttg ccgataagat gaagccggtc
      481 tggatcgtcg acgacgaccg ttccatccgc tgggtgatcg agaaagcgct cagccgggaa
      541 ggcatcgaat tcaactcgtt ttcctcggcg caggaagcgc tcgacgcgct ctcgggcgcc
      601 acccccgagg tgctcgtctc ggacatccgc atgccggggc gctcgggact cgagctgctc
      661 caggcggtga agcagcgcca tcccgcagtg ccggtgatcg tgatgacggc atattcggac
      721 ctcgacagcg cggtggcagc tttccagggc ggcgcttacg agtatctgcc caaacctttc
      781 gatatagacc aggcggtcga gctcatacgg cgcgcgctcg aggagcggcg tcgagactcg
      841 cacgaagcag agggtgcggt tgaaaacaag gaaatcctcg ggcaggcgcc cgcgatgcag
      901 gaggtgttcc gcgccatcgg ccggctgtcg caatcgagcg cgaccgttct gatcacgggc
      961 gagtcgggaa ccggcaagga gctcgtcgca cgcgccttgc accgccacag tgcgcgcgcg
     1021 cagcagccgt tcatcgcgat caacaccgcc gccatgccga aggacctgct cgaatccgag
     1081 ctcttcggcc acgagcgcgg ctcgttcacg ggcgcacagc agcagcgccg cggccgcttc
     1141 gagcaggccg aaggcgggac cctgttcctg gacgagatcg gggacatgcc tgccgagctg
     1201 cagacgcggc tcctgcgcgt gctctccgat ggcacgttct atcgcgtcgg gggccaccag
     1261 cagatcaagg tcaacgtgcg ggtgatcgcg gccacgcacc acaacctcga gcaacgcgtg
     1321 cgcgagggca gctttcgcga ggacctgtac caccgtctta acgtcatccg gctgcgcctt
     1381 cccagcctgc gcgagcgcgc cgaggacatt ccgttgttgg cgaagcattt cctcgcacgc
     1441 agcgcgcagc agctcggtgt cgagcccaag cgcatcgccg aggaggcgct tacccacatc
     1501 gcgcggctcg actttccggg caatgtgcgt cagctcgaga acctctgcca ctggctcacc
     1561 gtaatggcgc cggggcaggt gatcgatctc ggcaacctgc cgccggagtt ccgcagggag
     1621 ttgcccggcg atggcccggc cggcgccgcc gactggatcg cggcgctcga gcaggaagcc
     1681 gagcgccgcc tcgcgcgcgg cgataccggg atcctggacg cgcttgggcg tcagttcgag
     1741 cgtgcgctca tcagcaaggc gctcgcacgc accggcggcc ggcgcatcga cgccgcgaat
     1801 ctgctcggaa tgggccgcaa taccatcacc cgcaagatcc aggaactggg gatcgacgga
     1861 gacaaatcag gcaattcggg gacccattcg gggacggagc cctaacttac ttcgcaaagc
     1921 tccgaagcta gggctccgtc cccgaatcgc taagcggctt tcttgcggtt cgcctcgagc
     1981 ttcgcgtcca ccagcccgac cacttcgtcc agcgcgggta gccgcaggcc tttttgcgcg
     2041 gcgatcgcct gcaccagccg gtcgacacgc tcgatgtaag gcgcgccgcc gtagagcgcg
     2101 cgcgcggccg aggacgggct gccgaggctt tgcgcggcgg cggcgtactt gtcgaaggga
     2161 acgaggtcgg tttccttcgc gccgaggagc aggcacaggt tcaccaccca gttatacgcg
     2221 gcgcgcgtgg cattgacgtc agaatgcacg gcgtctttga tcgagcgtgg gccttcccgg
     2281 gtgacgcatc ggtaatttcc ggcgatcagc attgcccact tcgcgagcgg cacgaacacc
     2341 gactcgtgaa ccttgagctt gaccggcaac tccatctttt cgaagcgcgc cgcttcgatg
     2401 tccgcctcga gctttttcag aatcgcggta ttcgcctccg acgggaagca cgccgacttg
     2461 aaattcgtcg gcagccgcac ctggagtacg ttcaccttct cttcgggggg gcggaaagct
     2521 tgtggatccg ggctgcagag cgtcatgagg gccgggtcga agctgtccca gacgctcgca
     2581 tcggtgtagc aggacctcag ctctttggtg gacaccccgg gtatgcgcgc aaggtagggc
     2641 agcggcggca tgttcatgat cgacatgcag ggcaccttgg ccttcgcgat cgcatcgagg
     2701 agctcgcgca cgccggcgga tcggtattga ggctcctgca tcgcgagtgc gaccaggtcg
     2761 tattccgagg gcttgatcgt ttgcggaccg tcggcggaca gccttccggg cagcttcttc
     2821 gagtgcactt cgaccaagcc ttcgcgcccc ttcaccggca accgcacccg ggtcccgtcg
     2881 gtgttgatgg cgtcggcctc agccggcagg cacacaagct tcaccgaatg gcctgcgaga
     2941 agcagcttgg tcgcaagcag ggagccgtag gaggcgccca gaatcaggat ttggtacggt
     3001 ttcataatgt gaaatggatc cccgtggtct gaagttcagg ctactccgtt cgagccatga
     3061 attcatgttg cactgccaca tttcgcttaa taaaattgtg cgatgc
//
LOCUS       DASUNT010000606         2988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_254155, whole genome shotgun sequence.
ACCESSION   DASUNT010000606 DASUNT010000000
VERSION     DASUNT010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254155"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..564)
                     /locus_tag="VFX94_12275"
     CDS             complement(28..564)
                     /locus_tag="VFX94_12275"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX5769004.1"
                     /translation="MTPEQIQTANKANVETLLAVANAQFSVLEKLANISAGAVKSAFE
                     ESIANTRALLGAKDVQEFVTLQNSFAQPAIEKAIAYSKSLYEVATEANAELSKVAERR
                     VAEWNENFVSMLDKVSKNAPAGSDVAVAAIKSMLAAGNSAYDNLTKVAKQATEIAEAN
                     VAAATETVKGLAKSKKAA"
     gene            695..1909
                     /locus_tag="VFX94_12280"
     CDS             695..1909
                     /locus_tag="VFX94_12280"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5769005.1"
                     /translation="MATLKYHPNDPALLADPFPFYSRMREEDPVHWSPLLRAWVLTRY
                     DDVKRVCLDTAGMSSDRLRPFFATLAPPEALRVAELARYLTLWMVFRDPPEHTRLRRF
                     AAKVFHVRSIHALRPNIEALTQWLLDSIGGRREFDFIADFAGPLPALVIMDMLGVPRA
                     ELGRLKRLSDEMALFIGSARGAPEKYERAAQATREMADIFRDLVAARRKAPQRDLLSE
                     LIALDEGGERLSADELVATCMLLLFAGHETTTHHLANGLRALLAFPGELEKLRADRSL
                     APAAVEELLRFDGPIGAQVRIVREPLAFRGKALKPGERVFLLLNAANRDARAYPDPDR
                     LDLARNGVPHLTFGFGAHICLGFPLARLEGQIALPAVLARWRHVELAAEPEWLDSMVL
                     RGMKAMPVRVWG"
     gene            1906..2808
                     /locus_tag="VFX94_12285"
     CDS             1906..2808
                     /locus_tag="VFX94_12285"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5769006.1"
                     /translation="MSAEPDHLPQRLWWVLGSLTLAWGFNWTAMKVALAEVPPWTFRS
                     LCLGLGSAVLFAALRASGQRLALPTGQWRRLWLLALLNITSWNILVAFGVGMIPSGRA
                     ATLAYTMPVWAVPLSVWLLGERITGAKLVGLALGLAGLALLLAESFGGLGRAPLGSLL
                     VLGAALSWALGTVLQKRFPVQLPTGLYTAWIMLLGGVPIFAGALVFDDWEALRSVGAI
                     AWLGTAYNVVIAFAFAHWAWIKIATSVPVSVFSISMLLIPVVGVVSGMAFLGERPAWA
                     EYAALALVLVALLTVLRPGRAAAG"
     gene            complement(2771..>2988)
                     /locus_tag="VFX94_12290"
     CDS             complement(2771..>2988)
                     /locus_tag="VFX94_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX5769007.1"
                     /translation="TPRYLHIPAAVNAAGEKLSKQTGAAPIRRAKGVLQRALSFLGQR
                     ETENLDRAVRDWNPSLIPPQRALVAAP"
BASE COUNT          415 a          979 c          978 g          616 t
ORIGIN      
        1 cgttgctctg gctgcccggt ttggcgctta ggcggccttt tttgatttcg cgaggccctt
       61 caccgtctcg gtcgcggccg cgacgttcgc ttccgcgatc tcggtcgcct gcttcgcgac
      121 cttggtgagg ttgtcgtagg cggagttgcc ggcggcgagc atcgacttga tcgcggcgac
      181 ggcgacgtcc gagccggccg gggcgttctt cgacacctta tcgagcatgc tgacgaagtt
      241 ctcgttccac tcggcgacgc gtcgctcggc aaccttgctg agctcggcgt tcgcttcggt
      301 agcgacttcg taaaggctct tcgagtaggc aatggccttc tcgatggcgg gctgcgcgaa
      361 cgagttctgc agcgtcacga attcctgcac gtctttcgcg ccgagcaggg ctcgggtgtt
      421 ggcaatcgac tcttcgaagg cgctcttgac agcgccggcg ctgatgttgg ccagcttttc
      481 gagcaccgag aactgcgcgt tcgctacggc gaggagggtc tcgacgttcg ccttgttggc
      541 ggtttgaatc tgttcgggtg tcacgtacat ttccagtttc tcctgaggtc taagaaaatc
      601 tcttttgtgc attgcagcaa aagcgattgt agaccgatgg tctgtaaagt caagaatttt
      661 gtgcatagca gcatcgacta caaactgtga gttagtggcc actctcaaat accaccctaa
      721 cgatcctgcg ctgctggcgg atcctttccc gttttattcc cgcatgcggg aagaagaccc
      781 cgttcactgg agcccgctgc tcagggcttg ggtgctgacg agatacgacg acgtgaagcg
      841 cgtctgcctc gacaccgccg ggatgtcttc cgaccggctg aggccgttct tcgctacgct
      901 ggcgcccccc gaagcactac gcgttgccga gctcgcccgc tacctgacgc tttggatggt
      961 gtttcgcgac cccccggagc acacgcggct gcgccggttc gccgcgaagg tgttccacgt
     1021 gcgctcgatt cacgccctgc ggccgaacat cgaggcgctc acgcagtggc tgctcgattc
     1081 gattggcggg cgccgcgagt tcgacttcat cgctgatttt gccgggccgc tgccggcgct
     1141 cgtcatcatg gacatgctcg gcgtcccgcg cgcggagctc gggcgcctga agcggctgtc
     1201 cgacgagatg gcactcttca tcggaagcgc gcgcggtgcg ccggagaaat acgagcgcgc
     1261 ggcgcaggcg acgcgcgaga tggcggacat tttccgtgat ctcgtcgcag cgcggcgcaa
     1321 ggcgccgcaa cgcgacctgc tgagcgagct catcgcgctc gacgaaggcg gcgaacgcct
     1381 ctccgcggac gagctcgtcg cgacctgcat gctccttctt ttcgccgggc acgagaccac
     1441 cacgcatcat ctcgccaacg gcttgcgcgc tttgctcgca tttccgggcg agctcgagaa
     1501 gctgcgcgcc gatcgttcgc tcgccccggc ggccgtcgag gagctgctgc gcttcgacgg
     1561 gccgatcggg gcgcaggtgc ggatcgtgcg cgagccgctt gctttccgcg gcaaggcact
     1621 gaagccgggc gagcgcgtct tccttctctt gaacgcggcg aaccgggatg cgcgcgccta
     1681 cccggatcct gaccgcctgg atctcgcacg aaacggcgtg ccgcacctca cgttcggctt
     1741 cggcgcgcac atctgcctcg gcttcccgct cgcgcggctg gaagggcaga tcgcgctgcc
     1801 cgccgtgctc gcgcgctggc gccacgtcga gctcgccgcc gagccggaat ggctggattc
     1861 catggttctg cgcggaatga aagcgatgcc cgtgcgggtg tggggttgag cgccgaaccc
     1921 gaccacctgc cgcagcgcct gtggtgggta ctgggaagcc ttactctggc ctgggggttc
     1981 aactggaccg cgatgaaggt cgcgctggcc gaagtgccgc cgtggacgtt ccgcagccta
     2041 tgcctcgggc ttggctcggc ggtcttgttc gcggcgctgc gcgccagcgg gcagcgcctt
     2101 gccttgccga ccgggcagtg gaggagactt tggctactcg cgcttctcaa catcaccagc
     2161 tggaacatac tcgtcgcctt cggcgtaggc atgattccat cgggccgcgc cgcgaccctc
     2221 gcgtacacga tgccggtatg ggcggtcccc ctctccgtat ggctgctcgg tgagcgaatc
     2281 accggcgcga agctggtcgg tctcgcgctc ggtctcgccg ggcttgcgct gcttctcgcg
     2341 gaaagcttcg gcggcctcgg ccgcgcacca ctcggctccc tgctcgttct cggggcagcg
     2401 ctctcatggg ccctcggtac cgtcctgcag aagcgcttcc ccgttcagct gccgaccggg
     2461 ctctacaccg cctggatcat gctgctcggc ggcgtaccaa tcttcgcggg cgctctcgtg
     2521 ttcgacgact gggaggcgct tcgcagtgtt ggcgcgattg cgtggctcgg aacggcgtac
     2581 aacgtcgtca tcgcgttcgc attcgcgcac tgggcatgga tcaagatcgc gacctccgtt
     2641 ccggtgagcg tgttctccat cagcatgctg ctcatcccgg tggtcggcgt cgtgtcgggg
     2701 atggcattcc ttggcgagcg accggcctgg gccgagtacg ccgcgctcgc cctggtgctt
     2761 gtcgcgctgc ttacggtgct gcgaccaggg cgcgctgcgg cgggatgagc gaagggttcc
     2821 agtctctcac cgcccgatcc aggttctcgg tctcacgctg gccgagaaac gaaagcgccc
     2881 gttgcagtac gccttttgcg cgccgaatgg gcgctgcgcc tgtctgcttc gaaagctttt
     2941 ctcccgcggc gttcaccgcc gccgggatgt gcagataacg cggcgtcg
//
LOCUS       DASUNT010000607         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_342905, whole genome shotgun sequence.
ACCESSION   DASUNT010000607 DASUNT010000000
VERSION     DASUNT010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342905"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VFX94_12295"
     CDS             <1..1057
                     /locus_tag="VFX94_12295"
                     /inference="COORDINATES: protein motif:HMM:NF042664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HEX5769008.1"
                     /translation="RALTPPDVEFAAVSSHTALILALESIATRCKSDMPKPDLAEQRT
                     KIRYLQSVHAASWAGKGAVAEAFAAAWAEADDRKTAIEWYRKALVAEDSGASMKAVEQ
                     LLNLRVRLAAEDVDAARRTGDPAKLRALARDRAREARDAIENLEKLVEELSTMERINL
                     CGSAHKRLALILEAAGEADGAAIEAVRRHYAAAEELGAKSPDVFYPALNVIAVDVLAR
                     SGRVPAGRFKAVREAIDARLRDDPDFWSAAGDIELMLYEALNAAALAPKLAALESGLE
                     DLHARWPSKLMWSSVRDQARFVLPRYARRRGAPAEEDAALRLLTLLEAYAGDKTEIKP
                     RKGSGARTGQRARGRKS"
     gene            complement(996..1925)
                     /locus_tag="VFX94_12300"
     CDS             complement(996..1925)
                     /locus_tag="VFX94_12300"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX5769009.1"
                     /translation="MAKKSAKRKAKRQAISLHLGLNAVDPAHYEGWDGPLAACEFDAR
                     DMEALARSRGMSASVLLTRQATRARVLKAIRSAARRLKKGDFFFLSFSGHGGQVDDVS
                     RDEDDLGDQADKQDETWCLFDAQLIDDELHYELSRFAAGVRILVFSDSCHSGTVLRAA
                     LPITDPARPNARSRFMPPAIARRTYAAHREFYDRLQRDVEKRAGKAPVADPDAALAEV
                     AVSSRLTAIVKKFKPAAILISGCQDNQTSMDGERNGAFTEALLSVWNSGGFRGNYAQF
                     HASIKARLPATQTPNFFVLGPAARFVRQTPFAV"
BASE COUNT          340 a          740 c          768 g          340 t
ORIGIN      
        1 cagggcgctc acgccgcccg acgtggaatt cgcagccgtg agctcgcaca ccgcgctcat
       61 cctcgcgctc gagagcatcg cgacgcgctg caagtccgac atgccgaagc ccgacctcgc
      121 cgagcagcgc acgaagatcc ggtacctcca gtcggtgcac gccgcgagct gggccggcaa
      181 gggggcggtc gccgaggcgt tcgcggcggc ctgggcggaa gccgacgacc gcaaaaccgc
      241 gatcgagtgg tatcgaaagg cgctcgtcgc ggaggacagc ggcgcgtcca tgaaagccgt
      301 tgagcagctc ctgaacttgc gcgtgcgcct cgccgccgaa gacgtggacg cggcgcgccg
      361 cacgggcgac ccggcgaagc tgcgcgccct cgccagggac cgcgcccggg aggcgcgcga
      421 tgcgatcgaa aatctcgaga agctggtgga ggagttgtcc accatggagc gcatcaacct
      481 gtgcggctcg gcgcacaagc gcctcgccct gatcctcgag gcggccggcg aggccgacgg
      541 cgcggcgatc gaggcggtgc ggcgccatta cgccgcggct gaagagctcg gcgcgaagtc
      601 gcccgacgtt ttttacccgg ctttgaacgt gatcgcggtt gacgtgctgg cgcggtccgg
      661 ccgcgtgccc gccggccgat tcaaggcggt gcgcgaggcg atcgacgcca ggctgcgcga
      721 cgatccggat ttctggagcg cggcgggcga catcgagctc atgctctacg aggcgctcaa
      781 cgccgccgcg ctggcgccga agctcgccgc gctcgagtcc gggctcgagg acctgcacgc
      841 acgctggcct tcaaagctca tgtggagctc ggtgcgcgac caggcgcgct tcgtgctgcc
      901 gcgttatgcg cggcgtcgcg gcgcgccggc agaggaggac gcggctttgc gcctcctcac
      961 gctgctggag gcttacgccg gcgacaagac tgagatcaaa ccgcgaaagg ggtctggcgc
     1021 acgaaccggg cagcgggccc gaggacgaaa aagttaggcg tttgcgtcgc cggcaggcgt
     1081 gccttgatcg atgcgtggaa ctgcgcgtag ttgccgcgga agcccccgct gttccagacg
     1141 ctcagcagcg cctcggtgaa ggcgccgttg cgctcgccgt ccatcgaggt ctggttgtcc
     1201 tggcaaccgg agatgaggat cgccgccggc ttgaacttct tcacgatcgc ggtgaggcgc
     1261 gagctcaccg ctacttcggc aagcgccgca tccgggtcgg ccaccggggc cttgccggcg
     1321 cgcttctcga cgtcgcgctg cagccgatca tagaactccc tgtgcgctgc gtacgtgcgc
     1381 cgggcgatgg ccggcggcat gaagcgcgag cgcgcattcg gccgcgccgg gtcggtgatg
     1441 ggcagcgccg cgcgcagcac cgtgccgcta tggcagctgt ccgagaacac gaggatgcgc
     1501 acgcccgccg cgaagcggct gagctcgtaa tgcagctcgt cgtcgatgag ctgcgcatcg
     1561 aacaggcacc aggtctcgtc ctgcttgtcg gcctggtcgc cgaggtcgtc ctcgtcgcgg
     1621 ctcacgtcgt cgacctggcc gccgtggccc gagaagctga ggaaaaagaa atcgcctttc
     1681 ttgagcctgc gggcggcgct gcggatcgcc ttcagcacgc gcgcgcgcgt cgcctggcgg
     1741 gtgagcagca cgctcgccga catgccgcgc gagcgcgcga gcgcctccat gtcgcgcgcg
     1801 tcgaactcgc acgccgcgag cggcccgtcc cagccttcat agtgcgccgg atcgaccgcg
     1861 ttcagaccga gatgcagcga gatcgcctgg cgctttgctt tgcgcttcgc agatttcttt
     1921 gccatgtccg aaatctatga cgcgtgccca ggcgcagtaa acgtgaatat tggcttcttt
     1981 tccatgccgg cctggcacgc gaggcgcctg agctgatcgg tcgcatgcca gccttcgcgg
     2041 ggctcgaatg ggagcacaac ccggcagcgt atgcctgcgg cgcacgctgc gcctgcccgc
     2101 gagcgccgcg accgcgctca agtaattcgc cttagtattg gagctattgc gcgcacgctg
     2161 ggatctcgtc atggcaccaa cggcgctg
//
LOCUS       DASUNT010000608         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_867000, whole genome shotgun sequence.
ACCESSION   DASUNT010000608 DASUNT010000000
VERSION     DASUNT010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867000"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VFX94_12305"
     CDS             complement(<1..250)
                     /locus_tag="VFX94_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5769010.1"
                     /translation="MKKILVLLSTLAIVAAIAPFTGPFIILLLAQILVLGIIAMSVDL
                     LLGYTGLPSLGQAAYLGVGAYLTAILATKFGFGLGWDFW"
     gene            complement(247..1107)
                     /locus_tag="VFX94_12310"
     CDS             complement(247..1107)
                     /locus_tag="VFX94_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5769011.1"
                     /translation="MDANFWLIQTFNGVSYGALLFLLASGLTLIFGVMRIVNLSHGAY
                     FLLGGYVALTVIRGTGSWVLALPAAALTLAIVGLLMERIFLRPLGFDPLRQVLLTVGF
                     AFLFQQAALDIWGGDNLIIQPPAALSQSVVAGGLHLPVYRIFMIALAAAIGLALWFAM
                     ERTRIGAVVRATVDDAEMARGVGIDTSKVSMFIFALGAFLAALGGVIGGAFLGIYPGL
                     DFEVLPIAFAVVIIGGMGSLAGAAVGSMLVGLADNFGKALFPEVAYFSLYAPMVLILA
                     MKPTGLMGRE"
     gene            1148..2032
                     /locus_tag="VFX94_12315"
     CDS             1148..2032
                     /locus_tag="VFX94_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5769012.1"
                     /translation="MRPLERIAYSPIIQRAALRLPGGARMIVWTIVNVEEWDIDQPMP
                     RTGHTPPAGGAPMPDIPNWAWHEYGNRVGFWRLTEILDSLSIKAALAINGTAVRAYEP
                     ISRAALERGWEFMGHGYTQRNMQKVENERDDIAKTTEAIRALTGKRPRGWLGPGLTET
                     WETPDLLAEAGYDYVCDWVLDDQPVMLKTRTRPILNVPYTQECNDVAMMLIQHHEAGE
                     YYRRAMDQFEQLYADSRDSARVMAIVVHPYIMGAPHRAKYYRRALEEICAHKDVLFWT
                     GEQIADWYMEKGSGPFAR"
BASE COUNT          439 a          692 c          679 g          285 t
ORIGIN      
        1 gccagaagtc ccagcccaaa ccgaagccga acttggttgc gagaatcgcg gtgaggtaag
       61 cgccgacccc gagatacgcc gcctggccga gcgacggcag gccggtgtag ccgagcagga
      121 gatccacact catggcgatg atgccgagca ccaggatctg cgcgagcagc aggatgatga
      181 atggcccggt aaacggtgca atcgccgcaa cgattgccag tgttgaaagc aaaaccaaaa
      241 tcttcttcac tctcgtccca tcagcccggt gggcttcatg gcgagaatca gcaccatcgg
      301 cgcgtacagg ctgaagtacg ccacctcggg aaagagcgcc ttgccgaagt tgtcggcgag
      361 gcccaccagc atgctgccga ccgcggcgcc ggcgagcgag cccatgcccc cgatgatcac
      421 caccgcgaac gcgatcggca ggacctcgaa gtcgaggccc ggataaatgc cgaggaaggc
      481 gccgccgatc acgccgccga gcgcggcgag gaacgcgccg agcgcgaaga tgaacatcga
      541 taccttcgac gtgtcgatgc ccacgccgcg cgccatctcg gcatcgtcga cggtggcgcg
      601 caccaccgcg ccaatgcgcg ttcgctccat agcgaaccag agcgcgaggc cgatcgccgc
      661 cgcgagcgcg atcatgaaga tgcggtacac cggcagatgc agcccgccgg ccaccacgct
      721 ctgcgagagc gccgccggcg gctggatgat caggttgtcg ccgccccaga tatcgagcgc
      781 cgcctgctga aagaggaacg cgaagccgac cgtgagcagc acctggcgca gcgggtcgaa
      841 cccgagcggc cgcaggaaaa tgcgctccat caggagaccg acgatggcaa gtgtcaaagc
      901 cgccgccgga agcgcgagca cccaggagcc ggttccccgg atcacggtga gcgccacgta
      961 gccgccgaga aggaagtagg cgccgtgcga gaggttgacg atgcgcatca cgccgaagat
     1021 cagcgtgagg ccgctcgcaa gcaggaagag cagcgcgccg tacgacacgc cgttgaacgt
     1081 ctggatcagc cagaagttcg cgtccacgcg gttcctcctc cgggcgaaag agtagcatca
     1141 cttctccatg agacccctcg agcggatcgc ttattcgccc atcatccagc gcgcggcgct
     1201 caggcttccc ggcggcgcgc gcatgatcgt ctggaccatc gtcaacgtag aggaatggga
     1261 catcgaccag ccgatgccgc gcaccggcca cacgccgccc gcgggcggcg cgccgatgcc
     1321 cgatatcccg aactgggcgt ggcacgagta cggcaaccgc gtcggcttct ggcggcttac
     1381 tgaaattctt gattctctga gtatcaaagc ggcgctcgca atcaacggca ccgcggtgcg
     1441 ggcctacgag ccgatctcgc gcgcggcgct cgagcgcggc tgggagttca tgggccacgg
     1501 ctatacgcag cgcaacatgc agaaggtgga gaacgagcgc gacgacatcg cgaagaccac
     1561 cgaggcaatc cgcgccctca ccggaaagcg gccgcgcggc tggctcgggc ctggcctcac
     1621 cgaaacgtgg gagacgcccg acctgctggc cgaagcaggg tacgactacg tctgtgactg
     1681 ggtgctcgac gaccagcccg tgatgctcaa gacgcgcacc cggccgatcc tcaacgtgcc
     1741 atacacgcag gagtgcaacg acgtcgcgat gatgctcatc cagcaccacg aggcgggcga
     1801 gtactaccgc cgcgcgatgg accagttcga gcagctttat gccgactcgc gcgattcagc
     1861 gcgcgtgatg gcgatcgtcg tgcatcccta catcatgggc gcgccgcacc gcgcgaagta
     1921 ctaccggcgc gcgctcgaag aaatctgcgc gcacaaggac gtgctgttct ggaccggcga
     1981 gcagatcgcc gactggtata tggaaaaggg gtcaggaccc tttgcccggt gagctacgcg
     2041 cagctccgcg gaaaagggtc ctgacccctt ttcggcgtag catagacggg cagtc
//
LOCUS       DASUNT010000609         4119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_430000, whole genome shotgun sequence.
ACCESSION   DASUNT010000609 DASUNT010000000
VERSION     DASUNT010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430000"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..653
                     /locus_tag="VFX94_12320"
     CDS             24..653
                     /locus_tag="VFX94_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX5769013.1"
                     /translation="MAKIYINPLPVRIWHWLNALGFVVLILTGINIRYADVVGLMSFA
                     MAVQVHNTVGFILIANYLVWLVFYLFTDKIVVYHPELNPRIYFRDSLRQMLYYGYGIF
                     RGDANPHHASAYRKFNALQALTYQIVMILLVPTQFYTGVLLWDIERFGGSIALFGGVR
                     VVATVHVLLFIFFTGFIFVHPYLASLSKKPFVHYKAMITGYEEVEEKNP"
     gene            complement(734..2131)
                     /locus_tag="VFX94_12325"
     CDS             complement(734..2131)
                     /locus_tag="VFX94_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5769014.1"
                     /translation="MSARILVVDDEDIVLQSCRRALAGGERVVDTAASGAEALKRIEE
                     GGYDVVLLDIMMPDIDGLQVLQRVKESHPDIEVIMVTGLSQINTAVRSMKLGAFDYLP
                     KPFDPDELALVVDRAVERRRLLQQSMDPKMDAGAKYRFDNIIGSSPRMQEVFRLIAQC
                     APTNTTVLITGESGTGKELIARAIHYNSLRKDRPIVPVDCMSLSENLLESELFGHVKG
                     AFTGAIAHKRGLLAVAEGGSVFLDEVGNIPLSIQAKLLRVIQEREFKAVGDTRTQSAD
                     IRLICATNRDLRAMVAEGTFRDDLFYRINIFPIRVPALRERRDDIPALAMHFLKDCST
                     EIGKPVSRFSDDAMALIASYDWPGNVRELENAVHRAVILATDDVVRKAHLAGLGQGVP
                     ASAPRTGEELKRIKKAAREKSVEEIEKAFVLEALKRNGWNVTKSAEETGMQRANFQAL
                     MKKHGIRLRGQGRSE"
     gene            complement(2128..3630)
                     /locus_tag="VFX94_12330"
     CDS             complement(2128..3630)
                     /locus_tag="VFX94_12330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5769015.1"
                     /translation="MATLLAFAALVLYQEQSRRLSDAAEHVAQLSDVITRSTRFAMLH
                     VRPDYVHSIIKDVALQEHIDRVRIFNKEGTIIDSSDFAEIGLKVDRKAEGCSQCHQSE
                     RPLAAVPRHERARIFETTDGRRMLASMEVIRNEPSCSNAACHAHPAEQTVLGVLDIVY
                     PLEDIDRATRASALTMVALSAAFVLLASGLVSLFVHRLVYRPLRDLETGAKRLAAGNL
                     DEQIPVRGEDEFGRLAASFNSMTLALKDATRNLEEKIAERTRELRTAQAEAARGEKLA
                     SVGLLAAGIAHELNNPLTGVLTFSTLMRKKAQPGSQEAEDLDLVIRETKRCAAIIRRL
                     LDFAREKTPEKKFADLNRLIEETVRLVERPAHLADIAIRLELDAALPEVWMDPDLVKQ
                     VVMNMLVNAQHAIDHNGSITVRTRALADKVEISIIDTGCGIPEKDLTRIFDPFFTTKG
                     VGKGTGLGLSVSHGIVEAHGGSIDVTSAVGEGSTFRVHLPLQPASAGVGA"
     gene            complement(3697..>4119)
                     /locus_tag="VFX94_12335"
     CDS             complement(3697..>4119)
                     /locus_tag="VFX94_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX5769016.1"
                     /translation="TVAVGHGKKAARHIDAWLKDRTYEKPEKHEAASLDKLNPWYYSD
                     APKTVRPMLDVIRRQSTFDEVTGGLDETNALFEARRCLSCGNCFECDNCYGVCPDNAV
                     IKLGPGKRFEFNYEYCKGCGLCVAECPCGAIKMVPEAI"
BASE COUNT          623 a         1375 c         1355 g          766 t
ORIGIN      
        1 gctcacgaga cgaaaggcgc ccaatggcaa agatctacat taacccgctg ccggtgcgca
       61 tctggcactg gctgaacgcg ctcggcttcg tggtgctgat tctcacggga atcaacatcc
      121 gctatgccga cgtggtcggg ctcatgtcct tcgccatggc ggtgcaggtg cacaacacgg
      181 tcggcttcat cctgatcgcg aactacctcg tgtggctcgt gttctatctc ttcaccgaca
      241 agatcgtggt ctaccacccg gagctcaacc cgcgcatcta cttccgcgac agcctgcgcc
      301 agatgctcta ctacggctac ggcatcttcc gcggcgatgc caacccgcac cacgccagcg
      361 cgtaccgcaa gttcaacgcg ctgcaggccc tcacctacca gatcgtgatg atcctgctcg
      421 tgccgaccca gttctacacc ggagtgctcc tctgggacat cgagcgcttc ggcggctcga
      481 tcgcgctctt cggcggcgtg cgcgtggtcg cgacggtgca cgtgctgctc ttcatcttct
      541 tcaccgggtt catcttcgtg cacccgtatc tcgccagcct gtcgaagaag ccgttcgtcc
      601 actacaaggc gatgatcacc ggctacgaag aggtggagga gaagaacccc tagaggaaga
      661 aaacagaaaa aaatggggcc cccgccttcg cgggggtgaa gacagattgg gtccccgcct
      721 gcgcggggag ggcttattca gatcttccct ggccgcggag ccggatgccg tgcttcttca
      781 tgagcgcctg gaagttggcg cgctgcatgc cggtctcttc ggcgctcttg gtgacgttcc
      841 agccgttgcg cttgagcgcc tcgagcacga acgccttctc gatctcttcg accgacttct
      901 cgcgcgccgc tttcttgatt cgcttgagct cctcgccggt gcggggagcc gatgccggga
      961 cgccttggcc gaggccggcg aggtgcgcct tgcgcaccac gtcgtcggtg gcgaggatca
     1021 cggcgcggtg cacggcgttc tcgagctcac gcacgttgcc cggccagtcg tagctcgcga
     1081 tgagcgccat cgcgtcatcg gagaagcggc tcaccggctt gccgatctcg gtgctgcagt
     1141 ccttcaggaa atgcatggcg agcgccggga tgtcgtcgcg ccgctcgcgc agggcgggca
     1201 cgcggatcgg gaagatgttg atgcggtaaa acagatcgtc gcggaacgtg ccctcggcca
     1261 ccatggcgcg caggtcgcgg ttggtggcgc agatgagccg gatgtcggcg ctctgcgtgc
     1321 gcgtatcccc gaccgccttg aactcgcgct cctggatcac gcgcaggagc ttcgcctgga
     1381 tggaaagcgg gatgttgccg acctcgtcga ggaacacgct gccgccctcc gcgaccgcaa
     1441 gcagtccgcg cttgtgggcg atcgcgcccg tgaacgctcc cttcacgtgg ccgaagagct
     1501 cgctctcgag caggttctcg gagagcgaca tgcagtcgac cggcacgatc ggccggtcct
     1561 tgcgcaggct gttgtagtgg atggcgcgcg cgatcagctc cttgccggtg ccgctctccc
     1621 cggtgatcag caccgtggta ttggtgggcg cgcactgcgc gatcaggcgg aacacctcct
     1681 gcatgcgcgg gctcgagccg atgatgttgt cgaagcggta cttcgccccc gcgtccatct
     1741 tcgggtccat gctctgctgc agcaggcgcc ggcgctcgac cgcgcggtcc accacgagcg
     1801 cgagctcgtc gggatcgaaa ggcttgggca ggtagtcgaa ggcgccgagc ttcatcgagc
     1861 gcaccgcggt attgatctgc gacagcccgg tcaccatgat gacttcgatg tccggatgcg
     1921 actccttcac gcgctgcagc acctgcaggc cgtcgatgtc gggcatcatg atgtcgagga
     1981 gcaccacgtc gtagccgccc tcctcgatgc gcttcagcgc ctcggcgccg ctcgccgccg
     2041 tgtccaccac gcgctcgccg ccggcgagcg cgcgccggca gctctgcagg acgatgtcct
     2101 cgtcgtcgac gaccaggatc ctggcgctca tgcgccgact ccggcgctcg ccggctgaag
     2161 cggcaggtgg acgcggaaag tcgacccctc gccgacggcg ctcgtcacgt cgatcgagcc
     2221 gccatgcgcc tccacgatgc catggctcac cgagaggccg agcccggtgc ccttgccgac
     2281 gcctttggtg gtgaagaacg ggtcgaagat gcgcgtcagg tctttctccg ggatgccgca
     2341 gcccgtgtcg atgatcgaga tttccacttt atcggcaagc gcccgggtgc gcacggtgat
     2401 gctgccgttg tggtcgatcg cgtgctgcgc gttcaccagc atgttcatga cgacctgctt
     2461 gacgaggtcc gggtccatcc acacctcggg cagcgccgca tcgagctcga ggcggatcgc
     2521 gatgtcggcg aggtgcgccg gccgctccac caggcgcacc gtctcctcga tcaggcggtt
     2581 caggtccgcg aacttctttt ccggcgtctt ctcgcgcgcg aagtcgagca gccgccggat
     2641 gatcgcggcg cagcgcttcg tctcgcggat cacgagatcg aggtcctcgg cctcctggct
     2701 tcccggctgc gccttcttgc gcatgagcgt cgagaaggtg agcacgccgg tgagcgggtt
     2761 gttgagctcg tgggcgatgc cggcggcgag caggcccacc gaggcgagct tctcgccgcg
     2821 cgcggcttcc gcctgcgccg tgcgcagctc gcgcgttctt tccgcgatct tctcctccag
     2881 gttgcgcgtc gcgtccttga gcgcgagcgt catgctgttg aacgacgccg cgaggcgccc
     2941 gaactcgtcc tcgccgcgta ccggaatctg ctcgtcgaga ttgccggcgg cgagccgctt
     3001 ggcgccggtt tcgaggtcgc gcagcggccg gtacacgagg cgatgcacga agaggctcac
     3061 caggccggag gcaagaagca cgaaggcggc cgatagcgcc accatggtga gcgcgctcgc
     3121 gcgggtcgcg cggtcgatgt cctccagcgg atagacgatg tcgagcacgc cgagcaccgt
     3181 ttgctcggcc ggatgggcat ggcacgcggc gttggagcac gagggctcgt tgcggatcac
     3241 ctccatgctg gcaagcatgc gccggccgtc cgtggtctcg aagatgcgcg cgcgctcgtg
     3301 cctgggcacc gcggcgagcg ggcgctcgct ctggtggcac tgcgagcagc cttcggcctt
     3361 gcggtccact ttcaggccga tctcggcgaa gtcgctcgaa tcgatgatcg tgccttcctt
     3421 gttgaagatg cgcacgcggt cgatgtgctc ctggagcgcc acgtccttga tgatgctgtg
     3481 cacgtaatcc gggcgcacgt ggagcatggc gaagcgcgtg ctgcgcgtga tcacgtcgga
     3541 gagctgggcc acgtgctccg ccgcatcgct cagccggcgg ctttgctcct ggtacaacac
     3601 gagcgcggcg aatgcgagca gggtcgccat gagcgcgacg cccagacgga tcgcgacctt
     3661 gagccggagg gtcgtcgtgg cgcggctccc ggacgactag atcgcttcgg gaaccatctt
     3721 gatcgcgccg caagggcact cggccacgca cagcccgcac cccttgcaat actcgtagtt
     3781 gaactcgaag cgcttgcccg gcccgagctt gatcaccgcg ttgtcggggc acacgccgta
     3841 gcagttgtcg cactcgaagc agttgccgca cgagaggcac cggcgcgcct cgaagagcgc
     3901 attggtctcg tccagccctc cggtcacctc gtcgaaggtc gattgccggc ggatcacgtc
     3961 gagcatcggg cgcacggtct tcggtgcgtc cgagtagtac cagggattga gcttgtccag
     4021 gctcgccgcc tcgtgctttt cgggcttctc gtaggtcctg tccttcagcc aggcgtcgat
     4081 gtggcgggcc gccttcttgc catggcccac cgccaccgt
//
LOCUS       DASUNT010000610         2660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_954012, whole genome shotgun sequence.
ACCESSION   DASUNT010000610 DASUNT010000000
VERSION     DASUNT010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954012"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..802
                     /locus_tag="VFX94_12340"
     CDS             137..802
                     /locus_tag="VFX94_12340"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5769017.1"
                     /translation="MKRDLRANQKERTRQGLVAAAKELARAGAAPTVAEAGEHARVSR
                     ATAYRYFPTQESLLIEVSDVTPVVAPVEQMLASLTGDDTEERLAKLLDTFNPIVVAEE
                     VPLRTALRVYLDTWLEARRRGEVAPKVREGRRMRWLDVALEPARRQLPKAEWRRLRAA
                     LALTLSIDALVVMKDVAQLDDAEALSVLRWSALSLLRASLSTARAASRRASGQSPASR
                     SAR"
     gene            complement(867..>2660)
                     /locus_tag="VFX94_12345"
     CDS             complement(867..>2660)
                     /locus_tag="VFX94_12345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5769018.1"
                     /translation="LVLIWRRRGFGNVLYLWLGVVLVASIADLTLSNFSGGRYTIGWH
                     ASRANLVVSQCLLLAFLLADTAHEERRLARIAALAAYGGAVAVVLAAVHLRWFLDPWL
                     GTKVPFITIYGAVAIAVWFGGPGPAVLAMVLGYASANVRYISDHAGVSVAGPAESMTL
                     ALFALSCSLIIVLGEAMRRARDRYRVSEAEVKERARELQRADANKSRFLAVLSHELRN
                     PLAPLRTGLALLRMRAQNDPATAETHDMMERQITQLSRLIDDLLDVSRIDRGKLELRT
                     QRLAIATVLRTAVDTAKPNIDGQGHELVVSYPGEPLYVEGDLVRLAQVVSNLLNNAAK
                     FTPPKGRIELSACVENERLVVRVADNGIGIAPERLPEVFEMFVQLEPAGGAAGGLGLG
                     LTLARAIVRRHGGEIVALSRGRGQGAEFIVRLPLATGPPMVEEEPSLPSVQPALSSRR
                     RVLVVDDNVDAAQTLAAYLRMEGHRVESALDGEAALRIAEVLHPDIAFIDLNMPRMDG
                     VEVARRLRVTPWGRSARLVALTGLGQPSDIERTREAGFDEHITKPADLVYVSRLAAEK
                     IGVTERIGVRPPDSLGRVDPEVGGQHRAGRG"
BASE COUNT          429 a          919 c          918 g          394 t
ORIGIN      
        1 gcttcatagc ggaagtgctg gttgatgatc tcgtccattc gctgcggcgt cattgaccgc
       61 ctccttgcgg ttttcatgag acacatgtat cgtgacgata cgtatatctc ttgtcaagac
      121 gaagtaccct gaaatagtga aacgcgacct gcgcgcaaac cagaaggaac gcacccgcca
      181 ggggctcgtg gccgcggcga aggagctcgc ccgcgccggc gcggcgccca cagtagccga
      241 ggctggcgag cacgcgcgcg tctcgcgcgc gacggcgtac cgctatttcc ccacgcagga
      301 gtcgctcctc atagaggtca gcgacgtgac ccccgtcgtc gctccggtcg agcagatgct
      361 cgccagcctc acgggtgacg ataccgaaga gcgccttgcg aagctgctcg acaccttcaa
      421 cccgatcgtc gtggccgagg aggtgccgct gcgcacggcg ctgcgcgtct acctcgacac
      481 gtggctcgag gcccggcgcc gcggtgaagt cgcgcccaaa gtgcgggagg gccggcgcat
      541 gcgctggctc gacgtcgcgc tcgagcccgc gcgccgtcaa ctccccaagg cggaatggcg
      601 ccgcctgcgc gctgcgcttg ccctcaccct cagcatcgac gcgctcgtgg taatgaagga
      661 tgtcgcgcag ctcgacgacg cggaagcgct ttcggtcctg cgctggagcg cgctcagcct
      721 tcttcgcgcc agcctcagca cggcgcgggc ggcgtctcgg cgagcttcgg gtcaatcgcc
      781 cgcaagccgt tcggccaggt gagcttcgtg aagtccaccg aggtcggcag ccccatccct
      841 tcgtctgcgt gcaggtgccc gggacgctac ccgcgcccgg cgcggtgctg accgccgact
      901 tctggatcta ctctccctag agaatcaggg ggtctgaccc cgattctttc cgtgacgccg
      961 attttctcgg cggcgaggcg cgagacgtag accaggtccg ccggcttggt gatgtgctcg
     1021 tcaaagcccg cctcgcgcgt tcgctcgatg tcggaaggct gcccaaggcc ggtgagcgcc
     1081 acgaggcggg cgctgcgccc ccaaggcgtc acgcgcagcc tgcgcgcgac ttccacaccg
     1141 tccatgcgcg gcatgttgag gtcgatgaac gcgatgtccg ggtgcagcac ttcggcgatg
     1201 cgaagcgccg cctcgccgtc gagcgcgctc tcgacgcgat ggccttccat gcgcaggtag
     1261 gcggccaggg tctgcgcggc gtctacgttg tcgtccacca cgagcacacg ccggcgcgac
     1321 gagagcgcgg gctgcacgga cggcagcgag ggctcctcct ccaccatcgg cggcccggtg
     1381 gcgagcggca gcctgacgat gaactcggcg ccctggccgc gcccacggct gagcgcgacg
     1441 atctcgcccc cgtgccggcg cacgatggcg cgcgcaagcg tgagcccaag tccgagcccg
     1501 cccgcggcgc cgcccgcggg ctcgagctgc acgaacattt cgaacacctc gggcagccgc
     1561 tccggcgcga tgccgatgcc gttgtcggcg acgcgaacca ccaggcgttc gttctcgacg
     1621 caggccgaca gctcgatacg gcccttcggc ggggtgaatt tcgccgcgtt gttgagcagg
     1681 ttcgacacca cctgggcgag gcgcacgagg tcgccctcga cgtacagcgg ctcaccgggg
     1741 tagctcacga cgagctcgtg cccctgccca tcgatgttcg gcttggcggt atcgaccgcg
     1801 gtgcgcagca cggtggcgat ggcgaggcgc tgggtgcgaa gctcgagctt gccgcggtcg
     1861 atgcggctca cgtcgagcag gtcgtcgatc aggcgcgaga gctgcgtgat ctggcgctcc
     1921 atcatgtcgt gggtctcggc cgtggcgggg tcgttctgcg cgcgcatccg gagcagcgca
     1981 agccctgtgc gcagcggcgc cagcgggttg cgcagctcgt gcgacagcac tgcgaggaag
     2041 cggctcttgt tcgcatccgc ccgctgcagc tcgcgcgcgc gctccttcac ctcggcctcc
     2101 gacacacggt agcggtcgcg cgcgcggcgc atcgcctcgc cgaggacgat gatgagcgag
     2161 cacgacagcg cgaacagcgc gagcgtcatc gactccgccg gcccggccac cgacacgccg
     2221 gcgtgatcgg agatgtagcg cacgttggcg ctggcatagc cgagcaccat cgcgagcacc
     2281 gccgggccgg ggccgccgaa ccagaccgcg atggcgaccg cgccgtaaat cgtgatgaaa
     2341 ggaaccttgg tgccgagcca gggatcgagg aaccagcgca ggtggacggc ggccagcacc
     2401 accgccaccg cgccgccgta ggcagccagc gcggcgatgc gcgcgaggcg ccgctcctcg
     2461 tgcgcggtat cggcaagcag aaaagcgagc agcaggcatt gggagacgac gaggttcgcg
     2521 cgcgaggcgt gccagccgat ggtgtagcgc ccgccggaga agttggaaag cgtgagatcg
     2581 gcaatcgaag cgacgagcac gacgccgagc cataggtaaa gcacgttgcc gaaccctcgc
     2641 ctgcgccaga taagaacgag
//
LOCUS       DASUNT010000611         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1306694, whole genome shotgun sequence.
ACCESSION   DASUNT010000611 DASUNT010000000
VERSION     DASUNT010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306694"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFX94_12350"
     CDS             <1..289
                     /locus_tag="VFX94_12350"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX5769019.1"
                     /translation="VRLGGLEIELEHRGAVPGPVRIAIRPESIVLKASTNSGLRGVVR
                     KAAYLGTHMEYTVEAPIGPLFVVDRDVARPIAAGTEVAIGFSDHGVALVPS"
     gene            307..2627
                     /gene="hrpA"
                     /locus_tag="VFX94_12355"
                     /pseudo
     CDS             307..2627
                     /gene="hrpA"
                     /locus_tag="VFX94_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524161.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
BASE COUNT          441 a          903 c          890 g          393 t
ORIGIN      
        1 ggtgcggctc ggcggcctgg agatcgagct cgagcaccgg ggcgctgtcc cgggcccggt
       61 caggatcgca atccgccccg aatcgatcgt tctgaaggca agcaccaact cggggctgcg
      121 cggcgtagta aggaaggccg cctacctcgg tacgcacatg gagtacacgg tagaggcgcc
      181 gatcgggccg ctgttcgtcg tcgaccgcga cgtcgcccgg cccatcgcgg cgggcaccga
      241 agtcgcgatc ggcttctccg accacggcgt agcgctcgtt cccagctgag attcgatttc
      301 gatcccgcgc tgccgatcaa cgcccggcgc gacgagatcc gcgccgccgt ggaagcacac
      361 ccggtggtga tcgtgtgcgg cgagaccggc tcgggcaaga ctacgcagct tcccaagatg
      421 ttgctggaga tgaacccgcg gctgcgcatt ggccatacgc agccgcggcg catcgccgcg
      481 cgctcggtgg cggcgcgcat cgccgaagag ctcggcaccg agctcggcga gcgcgtgggc
      541 tacaaggtgc gcttccagga ccaggcggga agccggactt cgatcaagct catgaccgac
      601 ggcatcctgc ttgccgagac gcagggcgac cgcgagctgc gccactacga ccggctcatc
      661 atcgacgagg cgcacgagcg cagcctgaac atcgattttc ttctcggtta tctgaagcaa
      721 ctgctcgcca cccggcggga tctgaaagtc ctggtcacgt cggccaccat cgacgccgag
      781 cgcttttcga agcacttcga gggcgcaccg gtcatcgagg tgtcaggacg gctctacccc
      841 gtggagatcc gctaccgccc ggtcggcggc gacgccgagg acacgacgcg cgacgaagag
      901 gaagaggcgc tcgccggagc gctcgacgag ctctgccgcg aaggtccggg ggacatcctc
      961 gttttcctcc ccggtgagcg cgagatacgg gatgctgcgc aggtactgag aaagaacttc
     1021 cgcggcgaaa tcctgccgct ctattccagg ctgtccgccg cggagcagga ccgggtgttc
     1081 aagcccggaa aggagcgccg cgtggtgctc gccaccaacg tggcggagac ctcgctcacc
     1141 gtgccgcgca tccggtacgt cgtggacacc ggcgacgcgc gcgtcaagcg ctacagctac
     1201 cgcaacaagg tggagatgct gcgcgtggag ccgatctcgc aggccgcggc gcagcagcgc
     1261 gccggccgct gcggccgggt cgccgacggc gtctgcatcc ggctctactc tgaagaagac
     1321 ttcgcgaaac gcccggcgtt caccgatccc gagctcctgc gctcctcgct cgcctcggtc
     1381 atcctgcggg cgaagagcct tggcctgggc gaagtcgaga cttttccctt cctcgatccg
     1441 ccgagcccgc gcgcaatcgc cgacggctac gacctgctga ccgagctggg ggctgtagat
     1501 gaaaagaaga tactcacccc gattggaaac gagcttgcgc gccttccgct cgacccgcgc
     1561 gtcgcgcgaa tgctggtcgc ggcgcgcgag gagcgctgcc ttgcgcaggt gcgcatcatc
     1621 gcggcggcgc tctcggtgca ggacccgcgc gagcggccgc tcgagcgcgc cgccgccgcg
     1681 gacgagcgcc acgcgcgctt cgccgacgat cattccgact tcctcgcgta tctgaagctc
     1741 tggaagctgc aggaggagcc cgggctgcgg cggctgtgcc gcgagagctt cctttcctac
     1801 ccgcgcatgc gcgaatggcg cgacgtgcac gcccagctcg agcaggcgct cgactggccc
     1861 gagtccgcgg tgaagcccga gaagccggaa ggctaccgcg tgatccaccg ggcgctgctt
     1921 gccgggctac tgggcaacat tggcatgaaa gacgaggagg gcgcgtacac gggcgcgcgc
     1981 ggcatcaagt tctgggtgca tccgggctcg tggacgaaga agccgggcaa gtggatcgtc
     2041 gccgcggagc tggtggagac cactcgcctc ttcgcgcgca cggtcgcgtc gatcgagccg
     2101 cgctggctgg aggaaatcgg agcgcacctg ctgaaacgca gcctgagcga cccgcactgg
     2161 gagaaggatc gcggcgaggt ggtagccttc gagcgcggca cgctgtacgg cctgccggtc
     2221 tacgccaacc gccgcgtgcc gttcgcgccc cacgacccgg atctcgcgcg cgacatcttc
     2281 atccgctcgg cgctggtgga aggcgatttc gacacgcgcg ccccgttttt tgcgcacaac
     2341 cggcgcctgg tcgcggacat cgagcgtctc gagcacaagt cgcggcgccc ggacatcctg
     2401 gtggacaaag agctgatcca cgccttctac gacgagcgca ttccgcggga aatcaattcc
     2461 ggacagtcat tcgaacggtg gcgccgccag gccgaggcgg cgaggccgcg gctgctctac
     2521 ctcgcgcgcg aggacctgat gcgccacgag gcggcgggca tcaccaccga caacttcccg
     2581 ccgtcgctcg cgctcgggcc caaccgctat gcgctcgagt accactt
//
LOCUS       DASUNT010000612         2361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1306719, whole genome shotgun sequence.
ACCESSION   DASUNT010000612 DASUNT010000000
VERSION     DASUNT010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306719"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..1252
                     /gene="hemB"
                     /locus_tag="VFX94_12360"
     CDS             251..1252
                     /gene="hemB"
                     /locus_tag="VFX94_12360"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX5769020.1"
                     /translation="MNIYGSFPALRMRRMRRDEFSRRLMRETTLTAGDLIYPVFVLDG
                     KARTEKVASMPGVERMTVDRLLRVAEQCVKFGIPVLALFPAIDSGLKTADGREAFNPK
                     GLVPRAVAALKKRFPELGIMADVALDPYTTHGQDGVIDATGYILNDATLDILAKQALA
                     EAAAGVDIVAPSDMMDGRVGRIRTALEKAGHIHTKIMAYSAKYASGFYGPFRDAVGSA
                     KALGKADKRTYQLDPANSDEALREVGLDLAEGADMVMVKPGLPYLDVLRRVKDEFKAP
                     TYAYQVSGEYSMLRGAIERGWLPESCMLETLLAFKRAGADGILTYFALDAAKALSRG"
     gene            complement(1230..>2361)
                     /gene="dsbD"
                     /locus_tag="VFX94_12365"
     CDS             complement(1230..>2361)
                     /gene="dsbD"
                     /locus_tag="VFX94_12365"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF001419.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEX5769021.1"
                     /translation="NKWRGLALSLTYVLGMALAYALAGIAAAYSGSLLAAALQNVWVL
                     GAFAAVFVILALSMFGFYELRLPGFVHHHLHTAHKRLRGGQIASVAAMGVLSAVIVSP
                     CVAAPLAGALLYISQTRDVVLGGAALFAMALGMGVPLVAVGVSEGALLPKAGAWMDSV
                     RKFFGVLLIAVAIWIVSPVLPPMGQMLAWGALFIGSAMFLRAIDPLPDSASGWWRLLK
                     GVGIVALVAGIAFIIGAFSGAQDVLRPLAGISRTGPSAMPLPWIRVASLAELEGKLKA
                     PGKTVMLDFYADWCVSCKEMEAFTFSDPNVRAQLDRLLLLQVDVTANSEADKALLKRF
                     SLFGPPGIIFFDPHGREIKGLRVIGYQNPDRFLKTLSLATAP"
BASE COUNT          486 a          755 c          759 g          361 t
ORIGIN      
        1 cgccgaggac gtgctttttt cgagtgtgac aaagcagggc ctcgagcaat gtcgcggcgt
       61 gctcgagtcg tggctcgagc acgccgcaga aataaaaagc ccccggtaaa ggggatataa
      121 accgggggcg aaacgcctta acgttgaatt aaggcacccg ctcagggagg agaagcggga
      181 gacgatcaac tcgtccgaag gtaagattca gcccgccccg caaagttccc ccggtaacat
      241 ttggaaataa atgaacattt acggtagctt cccggcgctg cggatgcggc gcatgcgccg
      301 cgacgagttc tcgcgccgcc tgatgcgcga gaccacattg acggcggggg atctcatcta
      361 cccggtcttc gttctggatg gaaaagcccg cacggaaaaa gtggcttcca tgccgggcgt
      421 cgagcgcatg accgtcgacc ggctgctgcg cgttgccgaa caatgcgtga aattcggaat
      481 cccggtgctc gcgctcttcc ctgcaatcga ttccgggctt aagacggccg acggccgcga
      541 agcgttcaat ccgaagggac tggttccgcg cgccgtcgcc gcactaaaga agcgttttcc
      601 cgaactgggc atcatggccg acgtggcgct cgatccgtac acgacgcacg gtcaggacgg
      661 cgttatcgac gcaaccggat acatcctcaa cgacgcgacg ctcgatattc tggcaaagca
      721 ggcgctcgcc gaagctgctg caggtgtgga catcgtcgcg ccttccgaca tgatggacgg
      781 ccgcgttggc cggatccgca cggcgctcga aaaagccggt catatccaca ccaagatcat
      841 ggcgtactcg gcgaagtacg cgtccggctt ctatggcccg ttccgcgacg cggtcggctc
      901 ggcgaaggcg ctcggcaaag ccgacaagcg aacctaccag ctcgaccccg cgaactccga
      961 tgaggcgttg cgcgaggtcg gtctcgacct cgccgaaggc gccgacatgg tgatggtgaa
     1021 gccaggcctt ccgtacctcg atgtcctgcg ccgagtgaag gacgagttca aggctcccac
     1081 gtacgcgtac caggtttccg gcgagtactc catgctgcgc ggcgcaatcg agcgcggctg
     1141 gctgccggaa tcgtgcatgc tggagacgtt gctcgcgttc aagcgcgccg gcgccgacgg
     1201 catcctgacg tatttcgcgc tcgatgcggc taaggcgctg tcgcgaggct gagcgttttc
     1261 aagaagcgat cggggttctg atagccgatg acccgcaggc ccttgatctc gcggccgtgc
     1321 gggtcgaaaa agatgattcc cggggggccg aatagcgaga agcgcttgag cagcgccttg
     1381 tcggcttcgc tgttcgcggt gacgtcgact tgcaggagaa gcaggcggtc aagctgcgcg
     1441 cgcacgttcg ggtcggagaa cgtgaacgct tccatttcct tgcacgagac gcaccagtca
     1501 gcatagaaat ccagcatcac ggtcttaccg ggtgccttga gcttgccttc gagctcggcg
     1561 agcgaggcca cccggatcca cggcagcggc atggccgagg ggccagtcct ggaaattccc
     1621 gcaagcgggc gcagcacatc ctgcgcgcct gagaacgcgc cgatgatgaa cgcaatgccg
     1681 gcgaccaggg ccacgatgcc gacgcctttc aacagccgcc accaccccga ggccgagtcg
     1741 ggcagggggt ctatcgcacg caggaacatc gcggagccga taaaaagcgc gccccaagcg
     1801 agcatctgcc ccatcggcgg gagcaccggc gagacgatcc agatcgccac cgcgataagc
     1861 agcacgccga agaacttgcg cacgctgtcc atccaggcgc cggccttggg cagcagcgcg
     1921 ccctccgaca cccccaccgc tacaagcggc acgcccatcc cgagcgccat cgcgaaaagc
     1981 gcagcgccgc cgagcacaac gtcgcgagtc tgcgagatgt agagcaatgc gccggcgagc
     2041 ggcgccgcga cgcatgggct cacgatcacc gcagagagta cgcccatcgc tgccaccgag
     2101 gcgatctggc cgccgcgcag ccgcttgtgc gccgtgtgca gatggtgatg cacgaagccc
     2161 ggcaggcgta gctcgtagaa gccgaacatg gaaagcgcga ggatcacgaa cacggccgca
     2221 aacgcgccga ggacccaaac attttgcagg gccgcggcga gaagcgagcc cgagtacgcg
     2281 gccgcgatgc cggcaagcgc gtaggcgagc gccattccga gcacgtacgt gagcgaaagc
     2341 gcgagcccgc gccacttgtt c
//
LOCUS       DASUNT010000613         2097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_430208, whole genome shotgun sequence.
ACCESSION   DASUNT010000613 DASUNT010000000
VERSION     DASUNT010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430208"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VFX94_12370"
     CDS             complement(<1..752)
                     /locus_tag="VFX94_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Twin-arginine translocation pathway signal
                     sequence domain-containing protein"
                     /protein_id="HEX5769022.1"
                     /translation="MISRRDFLAASVAALFVPGLASAQASGYRRLLVLVELKGGNDGL
                     NAVVPYADDTYYRLRPRIAIRADDVLKLDRSTGLHPALEPLMPLWREGNLAIVQGVGY
                     PSPNLSHFRSIEIWDTASRSDQYLGEGWLARAFEAHPVPAQFAAGGVAVGGADMGPLA
                     GAARAIALTQPEVFLRQARLARPVGSGRNSALDHILRVEAGIVEAAARLDAGVQFATA
                     FPPGQFGNAVRVAAQLAANPSGVAAIRVSLGG"
     gene            complement(749..>2097)
                     /locus_tag="VFX94_12375"
     CDS             complement(749..>2097)
                     /locus_tag="VFX94_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEX5769023.1"
                     /translation="EDARHLLNRTGFDAQLREIDEFARLTRREGVERLLAGVQPEAST
                     PPPAWVKEWTDPRVVRAMADEERKAFVRLQIERALELKAWWLAEMLATRSPFTERMTL
                     FWHNHFASSLQKVRSPPLMHRQNALLRRHALGNFGELLHAVSRDPAMIVYLDSATNRR
                     GQPNENFARELMELFTLGEGKYSEQDIREAARAFTGWSIEPQTGEYRFRPRQHDDGVK
                     QVLGRAVRSGDEVLDVLLAQPATAEYLAARLWREFVSPQPKPEELARVAAAFRGSGYE
                     IRAAMRALLLTPAFWAPENRGVLIKSPTDLVVGTMRQFGIEVSDPLPFVFLMRALGQD
                     LLSPPNVKGWPGGEAWINSTTLLARKQYLERLLRVEDTRMLMQKNMDSLKPGARRMIQ
                     AIAELHFSARDWMAPFEGRETMVPVVLLAGAPAGNPSGSGIEYLRALLADPLYQLK"
BASE COUNT          331 a          713 c          729 g          324 t
ORIGIN      
        1 aagccgccga gcgaaacccg gatagccgcg acgcccgacg gatttgcggc aagctgcgct
       61 gccacgcgca ccgcgttgcc gaactggccg ggcggaaaag cggtggcgaa ctgcacgccg
      121 gcgtcgagcc gcgccgccgc ctccacgatg ccggcctcca ccctgagaat gtgatcgagc
      181 gcgctgttgc ggccgcttcc cacgggacgc gccaggcgcg cctgccgcag gaacacttcg
      241 ggctgggtga gcgcaatcgc gcgcgccgcc ccggcgagcg gtcccatgtc cgcgccgccg
      301 accgcaacgc cgcccgcggc gaactgcgcg ggaacgggat gcgcctcgaa cgcgcgcgcg
      361 agccagcctt cgccgaggta ctggtcgctt cttgaagcgg tgtcccagat ctcgatggag
      421 cggaaatgcg agaggttggg gctcgggtag cccacgcctt gcacaatggc gaggttgcct
      481 tcgcgccaga gcggcataag cggctcgagt gccgggtgca ggcccgtgct gcggtcgagt
      541 ttaagcacgt cgtccgcgcg gatcgcgatc cgcgggcgca gccggtagta cgtgtcgtcc
      601 gcgtaaggca cgacagcatt gaggccgtcg ttgccgccct tgagctcgac caggacgagg
      661 agccggcgat agcccgaagc ctgcgcggac gcgaggccgg ggacgaaaag cgcggcgacg
      721 ctggcagcaa gaaagtcgcg gcgggaaatc atttgagctg gtagagcgga tcggcgagga
      781 gcgcgcgcaa gtattcgatt ccgcttcccg acggattgcc ggcgggcgcc cccgcaagga
      841 ggacgaccgg taccatggtc tcgcgcccct cgaacggcgc catccagtcg cgcgccgaaa
      901 aatgcagctc cgcgatcgcc tgaatcatgc gccgtgcgcc gggcttgagg ctgtccatgt
      961 ttttctgcat gagcatccga gtgtcctcca cacgcagcag gcgttcgagg tactgcttgc
     1021 gcgcgagcag cgtggtcgag ttgatccacg cctctccccc cggccatccc ttgacgttcg
     1081 ggggcgagag caggtcctgg ccgagggcgc gcatgaggaa cacgaaaggc agcgggtcgc
     1141 tcacttcgat gccgaactgg cgcatcgtgc cgacgacaag gtccgtcggc gacttgatga
     1201 gcacgccgcg gttctcgggt gcccagaacg ccggcgtgag cagcagcgcg cgcattgccg
     1261 cccgtatctc gtagccgcta ccgcgaaacg cggcggcgac gcgcgccagc tcttcgggct
     1321 tgggttgtgg cgagacgaac tcgcgccaca gcctcgccgc gaggtactca gccgtcgccg
     1381 gctgtgccag cagcacatcg agcacctcgt cgccgctccg taccgcgcgt ccgaggacct
     1441 gcttcacgcc gtcgtcgtgc tggcgaggcc tgaagcggta ctcaccggtt tgcggctcga
     1501 tactccagcc ggtgaacgcg cgcgccgcct ccctgatgtc ctgttcgctg tacttgccct
     1561 cgcccaaagt aaagagctcc atcagctcgc gggcgaagtt ctcgttcggc tggccgcgcc
     1621 ggttggtggc cgaatcgagg tacacgatca tcgccgggtc ccgcgacacc gcgtgcagca
     1681 gctccccgaa gttgccgagc gcgtgcctgc gcagcagcgc gttctgccgg tgcatgagcg
     1741 gcggcgagcg caccttctgc aggctggagg cgaagtggtt gtgccagaag agcgtcatgc
     1801 gctccgtgaa cggggagcgc gttgcgagca tctcggcaag ccaccacgcc ttgagctcga
     1861 gagcgcgctc gatctgcagg cgcacgaacg ccttgcgctc ttcgtctgcc atggcgcgca
     1921 cgacgcgcgg gtcggtccat tccttgaccc acgcgggcgg cggggtgctc gcctcgggct
     1981 gcaccccggc aagcagccgc tcgaccccct cgcgccgggt cagtctggcg aattcgtcga
     2041 tctcgcgaag ctgggcgtcg aacccggtac ggttgaggag atggcgcgcg tcttcgg
//
LOCUS       DASUNT010000614         4450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_254444, whole genome shotgun sequence.
ACCESSION   DASUNT010000614 DASUNT010000000
VERSION     DASUNT010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254444"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1569)
                     /locus_tag="VFX94_12380"
     CDS             complement(160..1569)
                     /locus_tag="VFX94_12380"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5769024.1"
                     /translation="MALYLEHFGLSEPPFRITPHTDFFFDGADRGATLEGLIYAVLHD
                     EGIVKVSGEVGSGKTMLCRVLMERLPQHVATIYLATPALGRDEILLAIADELNLKISP
                     ERRSVALRELQEQLIALYGAGRRVVVLIDEAHVMPDDTLEQVRLLSNLESNRHKLLQI
                     VLFGQPELDETLAKPALRQLRDRITHAFRTRPLAVTEVAKYLSFRMRAAGYRGPEVFT
                     PRAARLIARASGGLTRRINILADKALLAAYTEGTHGIVPRHVRAAIADSEFAAPRKPR
                     RIGYIAAAAAAGLAIGAAAQWIMTPLAPPARPVSAPAKMAALQQPPPAGPAPNVELQA
                     APPEAPPEPRLSEGQARRLAGYSPGGQKILADRLAASRALLERVDDERYALELFLTEN
                     TDPARMERFLLRAREIVPLEALYVVPIAAGGQYRLWVLFGDFASREEAVAASGGLPPQ
                     YKQAFRVAPRSFGDLRKQL"
     gene            complement(1572..2957)
                     /locus_tag="VFX94_12385"
     CDS             complement(1572..2957)
                     /locus_tag="VFX94_12385"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5769025.1"
                     /translation="MSTPLPSPKEKPGLLIVDDDPLITETLAFALGTAYTVYACESRA
                     HAIELLRQLEAPPPLALVDLGLPPTPHAPDEGFRLIAELLAHSPRMKIFVLSGQNEAA
                     NARHARALGAWDFIAKPADPASLDRALARGLEAPAAEDAEFVGASPALTKLKDQIAQF
                     AAAPYPVLIEGESGSGKELVAKRLHRLSPRSGRPYFSLNCAAISPQLVEPTLFGHVKG
                     AFTGAAATKAGFFEDAADGTLFLDEIGELPLDMQAKLLRVLENGEYQRVGETQLRTAR
                     CRVLAATNRDLRRAVREGSFRADLYHRLSVFTLSVPALRELEQDKLLLLDHFRRQSDR
                     PFQLDEAASERWLRYDFPGNVRELRNIVIRLSAKYPGQRLSVAELEPELDVDIGDDAG
                     RNDAVFEQALRQLERGGQFDLDQTLRAWERAYIQAALRLTRGNVSQAAKLLGINRTTL
                     YSRMNSAEEKK"
     gene            complement(2932..3678)
                     /locus_tag="VFX94_12390"
     CDS             complement(2932..3678)
                     /locus_tag="VFX94_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5769026.1"
                     /translation="MKGETPALRVTALVKRYGKFEALAGVDLELAAGDAFGLVGANGA
                     GKTTLIKCMLDLAACDAGAVHIFGVPALRPAARRRLAYLPERFNPPYYLRAGEFLAAM
                     FELAGERYDASRVESLLCSLELERAVLGQPVRTLSKGMTQKLGLAGCFAVPRDMYVLD
                     EPMSGLDPAARVAVKSVLRQLATEGRTLFFTSHVLADVEELCSSIAVLERGRVRFRGT
                     AAALCRRYSESSLERAFMKCIRDEHAVAVS"
     gene            3697..>4450
                     /locus_tag="VFX94_12395"
     CDS             3697..>4450
                     /locus_tag="VFX94_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5769027.1"
                     /translation="MPQDPIVQVKDVSFGYDPARPVLRDIELAIARGAVVGIMGQSGC
                     GKTTLLRVIMGALRPSRGTARVLGRLTTELDTPGLYEMRRKMGMLFQFGALFTDMSVY
                     ENVAFPLREHTDLPADLIHDLVMLKLQAVGLRGAAHLMPTELSGGMSRRVALARSIAL
                     DPELILYDEPFTGLDPISFGVIARLIRSLNDSLGATSIIVTHDVQEALNVVDYAYFMA
                     EGKVIAQGTPEQIRASPEPFVNQFVNGKPDGPV"
BASE COUNT          699 a         1502 c         1547 g          702 t
ORIGIN      
        1 gcaaccgctc accaagttca ccgacagcaa ggccacgctc gtcagcgcga gcgctctccg
       61 catcttcatg cgcggcattc cctcccgcga cgaaggataa cacgatgctt gtgtgaagaa
      121 agtcgcgtaa gcgcctgtcg cgcaggcgtt ttcggtgtct tacaactgct tgcgcaaatc
      181 gccgaagctg cgcggcgcga cgcgaaaggc ctgcttgtat tgcggcggca gaccgccgct
      241 cgcggcaacc gcctcttccc ggctcgcgaa atcgccgaaa aggacccaca accggtactg
      301 accgccggcg gcgatcggga ccacgtacag cgcttccagc gggactattt cacgcgcgcg
      361 cagcagaaac cgctccatgc gcgccggatc ggtgttctcg gtgagaaata gttcaagcgc
      421 gtagcgctca tcgtcgacgc gctcgagcaa cgcccggctg gccgcgagcc gatcggccag
      481 gattttctgc ccgcctgggg aatagccggc gagccggcgc gcctggcctt cgctcaatcg
      541 cggttcgggc ggcgcctcgg gcggtgcagc ctgcagctct acgtttggtg ccgggcccgc
      601 cggtggaggc tgctgcagag ccgccatctt cgcgggcgca gagaccgggc gcgcgggcgg
      661 cgcgagcggc gtcatgatcc actgagccgc cgcgccgatc gccagccccg ccgccgcggc
      721 cgcggcgatg tagccgatgc ggcgcggttt cctgggagcg gcgaactcgg agtccgcgat
      781 cgcggcgcgt acatggcgcg ggacgatccc atgggtgccc tctgtatagg cagcgagcag
      841 cgctttgtcg gcgaggatgt tgatgcgccg ggtgagcccg ccagaggcgc gagcaataag
      901 gcgcgctgcg cgcggggtga aaacctcggg tccgcggtag cccgcggcgc gcatgcggaa
      961 cgagaggtat ttcgccactt ccgtgaccgc gagcgggcgc gtgcggaagg catgagtgat
     1021 gcggtcgcgc agctgccgca gcgcgggctt ggcgagcgtt tcgtcgagct ccggctggcc
     1081 gaaaagcacg atctgcagga gcttgtgccg gttcgactcg agattcgaca gcagccgcac
     1141 ctgctcgagc gtgtcgtcgg gcatgacgtg cgcctcgtcg atcagaacca ccacgcgtcg
     1201 ccccgcgccg tacagcgcga tcagctgctc ctgcagctcg cgcagcgcga ccgaacgccg
     1261 ctccggcgaa atcttcaggt tgagctcgtc ggcgatcgcg agcaggatct cgtcgcgccc
     1321 gagcgcgggc gtggcgagat agatcgtcgc gacatgctgc ggcagccgtt ccatcagcac
     1381 gcggcacagc attgtcttgc cgctgcccac ctcgcccgac accttgacga tgccctcgtc
     1441 gtgcagcacg gcgtagatca gcccttcgag cgttgcgccg cggtcggcac cgtcgaagaa
     1501 gaagtcggtg tgcggcgtaa tgcggaacgg gggttccgac agcccgaagt gctccaggta
     1561 gagggccacg gctacttctt ctcttctgcg ctgttcatgc ggctgtataa cgtggtgcgg
     1621 ttgatgccga ggagtttggc cgcctggctc acgttaccgc gcgtcagccg cagcgccgcc
     1681 tggatatagg cgcgctccca cgccctgagc gtctggtcga gatcgaactg cccgccgcgc
     1741 tcgagctgcc gcagcgcctg ctcgaagaca gcgtcgttcc ggcccgcatc gtcgccgatg
     1801 tcgacgtcga gctcgggctc gagctcggcc accgagagcc gctgcccggg gtacttggcc
     1861 gagagccgga tcacgatgtt gcgcagctcg cgcacgttgc ccgggaaatc gtagcgcagc
     1921 cagcgctcgc tcgccgcttc gtcgagctgg aacggccggt cggactggcg gcggaagtga
     1981 tcgagcagga gcagcttgtc ctgctccagc tcgcgtagcg ccggtacgct cagcgtgaag
     2041 accgagaggc ggtggtacag atcggcgcgg aacgagccct cgcgcaccgc gcggcgcagg
     2101 tcgcgattcg ttgcggcgag gacgcggcag cgggcggtgc gcagctgggt ctcgccgacg
     2161 cgctggtact cgccgttctc cagcacgcgc agcagcttcg cctgcatgtc gagcggcagc
     2221 tcgccgatct cgtccaggaa aagcgtcccg tcggccgcgt cctcgaaaaa cccggccttg
     2281 gtggcggccg cgccggtgaa ggcgcccttc acgtggccga agagcgtggg ctcgacaagc
     2341 tgcggcgaga tagcggcgca gttcaggctg aaatacggcc ggcccgagcg cggcgagagc
     2401 cggtgcagcc gcttcgcgac gagctccttg ccgctgcccg actcgccctc gatcagcacc
     2461 gggtagggcg cggcggcaaa ctgcgcgatc tggtctttca gcttcgtgag cgcggggctt
     2521 gcgccgacga attcggcgtc ttccgccgca ggcgcctcca gcccgcgcgc gagcgcgcgg
     2581 tcgaggctcg ccgggtcggc gggcttggcg atgaaatccc aggcgcccag ggcacgcgcg
     2641 tggcgcgcgt tggccgcctc gttctgcccc gacagcacga aaatcttcat gcgcggcgaa
     2701 tgcgcgagga gctcggcgat caggcgaaag ccctcgtccg gagcgtgcgg cgtgggcggg
     2761 agcccgaggt cgacgagcgc gaggggcggc ggcgcctcga gctggcgcag aagctcgatc
     2821 gcgtgcgccc gggactcgca ggcgtagacg gtgtacgcgg tgccgagcgc gaaggcgagc
     2881 gtctcggtga tcagcggatc gtcgtccacg atcagcagcc cgggcttctc tttaggagac
     2941 ggcaacggcg tgctcatcgc ggatgcattt cataaaggcg cgctccaggc tcgactcgct
     3001 gtagcgccgg cacaaggccg ccgcagtgcc ccggaagcgc acccgaccgc gctcgagcac
     3061 cgcgatcgag gagcagagct cctcgacgtc ggcgagcacg tgggacgtga agaagagcgt
     3121 acggccctcg gtggccagtt gccgcagcac ggatttcacc gccacccggg cggcggggtc
     3181 aaggccgctc atcggctcat cgagcacgta catgtcgcgc ggcacggcga agcagccggc
     3241 gagcccgagc ttctgcgtca ttcctttgga gagggttcgc accggctggc cgaggaccgc
     3301 gcgctcgagc tccaggctgc aaagcagcga ttcgacgcgg gaggcgtcgt agcgctcgcc
     3361 ggcgagctcg aacatcgcgg cgaggaactc gcctgcacgc aggtaatacg gcgggttgaa
     3421 gcgctccggc agataggcga ggcgccgccg ggcggcgggc cgcagcgccg gcacgccgaa
     3481 aatgtgtacc gcgccggcgt cgcacgcggc gaggtcgagc atgcatttga tgagcgtcgt
     3541 cttgccggcg ccgttcgcgc ccaccaggcc gaacgcatcg ccggcggcga gctcgagatc
     3601 gacgccggcg agcgcttcga atttgccgta acgtttgacc agcgccgtca cgcgcagcgc
     3661 cggtgtctct cccttcatgc ttgataatat tacgccgtgc cgcaagatcc gatcgtgcag
     3721 gtgaaggacg tctccttcgg ttacgacccg gcgcgtccgg tgctgcgcga catcgagctc
     3781 gcgatcgcgc gcggcgcggt cgtcggcatc atggggcagt cgggctgcgg caagacgacg
     3841 cttcttcgcg tgatcatggg tgcgctgcgt ccttcgcgcg gcacggcgcg cgtgctcggg
     3901 cgcctcacca ccgagctcga cacgccgggg ctgtacgaaa tgcggcgcaa gatgggcatg
     3961 ctcttccagt ttggagcgct ctttaccgac atgtcggtgt acgagaacgt cgcgttcccg
     4021 ctgcgcgagc ataccgacct tcccgcagac ctgatccacg atctcgtgat gctcaagctc
     4081 caggcggtgg gcctgcgcgg cgcggcgcac ctcatgccca ccgagctctc ggggggaatg
     4141 tcgcgccgcg tggcgctcgc gcgctccatc gcgctcgacc cggagctcat tctctacgac
     4201 gagcctttca ccggactcga tccgatctca ttcggcgtca tcgcgcgcct gatccgcagc
     4261 ctcaatgact ctctcggcgc tacctccatc atcgtcacgc acgacgtgca ggaggcgctc
     4321 aacgtggtcg attacgcgta cttcatggcc gagggcaagg tgatcgcgca gggcacgccg
     4381 gagcagatcc gcgcgagccc cgagcccttc gtcaaccagt tcgtcaacgg caagcccgac
     4441 ggcccggtgc
//
LOCUS       DASUNT010000615         4656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_167642, whole genome shotgun sequence.
ACCESSION   DASUNT010000615 DASUNT010000000
VERSION     DASUNT010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167642"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..108
                     /locus_tag="VFX94_12400"
     CDS             <1..108
                     /locus_tag="VFX94_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEX5769028.1"
                     /translation="VPDPYYGGAAGFERVLDLVEEHCRSLLDELKRSTS"
     gene            complement(98..2647)
                     /locus_tag="VFX94_12405"
     CDS             complement(98..2647)
                     /locus_tag="VFX94_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX5769029.1"
                     /translation="MKRMLFNATQQEELRVAIVDGQKLVDLDIESASREQRKSNIYKG
                     IITRIEPSLEAAFVDYGEERHGFLPFKEVSRSYFKADIDVGRARIQDALREGQELIVQ
                     VEKDERGTKGAALTTFISLAGRYLVLMPNNPRGGGVSRRVEGEERNELRDTLDQLQLP
                     DGMSVIARTAAIGRSLEELSWDLNYLMQLWRAIEAEASKSTGKPFLIYLESSLVIRAI
                     RDYFQPDIGEILIDTEEIFEQAQTFMQTVMPGNVGKVKLYRDDIPLFSRFQIEHQIES
                     AYSRQVALPAGGAIVIDHTEALVAIDVNSARATRGGDIEETAFRTNLEAAEEIARQLR
                     LRDLGGLIVIDFIDMESQRNQREVENRLRDALKFDRARVQIGKISRFGLMELSRQRLQ
                     ASLEETAHISCPRCSGTGFIRGTESTALHVLRIIQEEAMKENTGAVHAQVPVDVSSFL
                     LNEKRAEIQKLEGRLKVNIVLVPNPHLETPHYKVQRLKHDELNQMEHVPASYELVEQP
                     EEPKAPGAEEESRRERQEAAVKGITPLQPAPVVDQSPPPVPQPLASPPPQPLASPPPQ
                     PQPAVQPAAEGGLLAKLFGLFKPAASALPPVAAAQPAAQPAQPQPRPDQQPRREHQHR
                     REPRAEHQRGDGRDRGRDRNREGRPRDQHRDRQQQGRLEQRRNGQPPQQQRQQRPPQQ
                     QQQRPQQQPQPPQQQPPQQQRDGEGERRGGGRRNRRDRHRERREERPANAPSFVIKEE
                     QLREDQRREALARKEEPHGEDALPPPPAPRVEEPAARVQAEAAPRIEQPNIDAKTLLE
                     DDGLVMIETDRSKAPPAPPVEETPHLGRPRRERPKPSSSQEEELQQVETRK"
     gene            2909..3802
                     /locus_tag="VFX94_12410"
     CDS             2909..3802
                     /locus_tag="VFX94_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX5769030.1"
                     /translation="MNDLSKAGARLKATWVEVGEESEAQRIDNFLLRKLKGVPKSHVY
                     RVLRSGEVRVNSGRVKPDYRLRAGDRIRVPPIRVAEGQRAARPHEFPVVYEDASLLVI
                     DKPAGVAVHGGSGVSYGVIESLRAARPQAKLLELAHRLDRDTSGLLMLAKKRSALVEL
                     HRMLREGEVEKIYLAVVNGEYKGPAEIRAALHKYVTGSGERRVAVTEDGRSALTRVKV
                     LRRNAESSLLEVRLLTGRTHQIRVHLAHVGHPIFGDEKYGGGEAKRLLLHAAKLAFRH
                     PLTGEPMRLASPAPAEMKAAL"
     gene            3802..4449
                     /locus_tag="VFX94_12415"
     CDS             3802..4449
                     /locus_tag="VFX94_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX5769031.1"
                     /translation="MKYRLLVFDWDGTIIDSASTIAECIREASRSLGLQVPGDERARH
                     VIGLGLHDAMRIVVPELPASRYPEFVASYRAHFLARKDSMQLFAGMRELLEELCGRHL
                     LAIATGKSRAGLDRDIDFHGLKALFAASRCADETHPKPHPAMLMELMDELAVPNDAAL
                     MIGDTSHDLEMARAADVGALAVSYGAHAEHGLRACGPLGCFSSVGELKAWLAANG"
     gene            4428..>4656
                     /locus_tag="VFX94_12420"
     CDS             4428..>4656
                     /locus_tag="VFX94_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX5769032.1"
                     /translation="MAGGERLICAGAALAEAGRGVRFEVEYFGEPAPAFVVRKNGAVH
                     GYLNRCAHVAMELDWQEGTFFDADGRDLLCST"
BASE COUNT          654 a         1531 c         1647 g          824 t
ORIGIN      
        1 gtgcccgatc cttactacgg cggcgccgcc ggcttcgagc gcgtgctcga tctcgtcgag
       61 gagcattgcc gctcgctcct cgacgagctg aaacggtcta cttcctagtc tcgacttgct
      121 gcagttcctc ctcctgcgac gacgagggct tcggccgctc gcggcgcgga cgaccgaggt
      181 gcggtgtctc ttcgacgggc ggggcaggcg gggctttcga ccggtctgtc tcgatcatga
      241 ccagaccgtc gtcctccagc agcgtttttg catcgatgtt aggctgctcg atgcgcggtg
      301 ccgcttcggc ctgcacgcgc gcagccggtt cttcgacgcg gggggcaggc ggcggcggga
      361 gcgcgtcctc gccgtgcggc tcctccttgc gcgcgagcgc ttcgcgccgc tggtcctcgc
      421 gcagctgctc ctcttttatg acgaaggaag gcgcgttcgc cggccgctcc tcgcggcgct
      481 cgcgatggcg gtcgcgccgg ttgcgccttc cgcctccccg gcgctcaccc tcgccgtcgc
      541 gctgctgctg cggcggctgc tgctgaggcg gttgtggctg ctgctgaggc cgttgttgct
      601 gctgctgagg cgggcgttgc tgccgctgtt gctgtggcgg ctggccattg cggcgctgct
      661 cgagccggcc ctgctgctgg cggtcgcgat gctggtcgcg cggacggcct tcgcgattgc
      721 ggtcgcgacc gcgatctctg ccatcgccac gctggtgctc ggcgcgcggc tcgcggcggt
      781 gctggtgctc gcgtcgcggt tgctggtccg gccggggctg cggctgtgca ggttgcgcgg
      841 cgggctgggc ggcggccaca ggcggcaggg ccgaagccgc cggcttgaaa agcccgaaca
      901 gcttcgcaag caggccgcct tcggcggcag gctgcaccgc gggctgcggc tggggcgggg
      961 gcgaggcgag cggctggggc gggggcgagg cgagcggctg cggcacgggc ggcggcgact
     1021 ggtccaccac cggcgcgggc tgaagcgggg taatgccctt gacggccgct tcctgccgct
     1081 cccgcctgct ttcctcctcg gcgccagggg ccttgggctc ttcgggctgc tcgacgagct
     1141 cgtagctcgc cggcacgtgc tccatctggt tcagctcgtc gtgcttgagg cgctgcacct
     1201 tgtagtgcgg cgtctcgagg tgcgggttgg ggacgagcac gatattgacc ttcaaacgcc
     1261 cctcgagctt ctggatctcg gcgcgcttct cgttcagcag gaaagaggag acatcgaccg
     1321 gcacctgcgc gtgcaccgcg ccggtgttct ccttcatcgc ttcttcctgg atgatgcgca
     1381 gcacgtggag cgcggtggac tcggtgccgc ggatgaagcc ggtgccgctg cagcgcgggc
     1441 agctgatgtg cgcggtttcc tcgagcgagg cttgcaggcg ctggcgcgaa agctccatca
     1501 gcccgaagcg cgagatcttt ccgatctgca cgcgcgcgcg gtcgaacttg agcgcgtcgc
     1561 gcaggcggtt ctccacctcg cgctggtttc gctgcgactc catgtcgatg aagtcgatga
     1621 cgatgaggcc gcccaggtcg cgcaggcgaa gctggcgcgc gatctcctcg gcggcttcga
     1681 ggttggtgcg aaaggcggtc tcctcgatgt cgccgccccg ggtagcgcgc gccgagttga
     1741 cgtcgatcgc gacgagcgcc tcggtgtggt cgatgacgat cgccccgccg gcgggcaggg
     1801 ccacctggcg cgaataggcg ctctcgatct ggtgctcgat ctggaagcgc gagaaaagcg
     1861 ggatgtcgtc ccggtagagc ttcaccttgc cgacgttgcc gggcatgacc gtctgcatga
     1921 acgtctgcgc ctgctcgaag atctcctcgg tgtcgatcag gatctcgccg atgtcgggct
     1981 ggaagtagtc gcggatcgcg cggatcacca ggctggactc gaggtagatg aggaagggct
     2041 tcccggtgga cttggacgcc tcggcctcga tcgcgcgcca gagctgcatc aggtagttca
     2101 ggtcccagct cagctcctcg agcgagcggc cgatcgccgc ggtgcgcgcg atcacgctca
     2161 ttccgtcggg gagctgaagc tggtcgagcg tatcgcgcag ctcgttgcgc tcttcgccct
     2221 cgacccggcg cgacacgccg ccgccgcgcg ggttgttcgg catgagcacg aggtagcgtc
     2281 ccgccaggct gatgaacgtg gtcagcgcag cgcccttggt gccgcgctcg tccttctcga
     2341 cctggacgat cagctcctgg ccttcgcgca gcgcatcctg gatgcgggcg cggcctacgt
     2401 cgatgtcggc cttgaaatag ctgcgcgaaa cttccttgaa cggcaggaac ccgtgccgct
     2461 cctcgccgta gtcgacgaac gcggcctcga ggctgggctc gatgcgggtg atgattccct
     2521 tatagatgtt gctcttgcgt tgctccctgg atgcggactc gatgtcgagg tcgacgagct
     2581 tttgcccatc gacgatcgcg acgcgcagct cttcctgctg cgtcgcgtta aacaacatgc
     2641 gcttcacttt ttgttctcct gcgcgcgcag gagagaggtg tcagccggct gaaaaaatct
     2701 cctcttgggg atgacctctt cgggtcctct gagacgtccg gggaaaagcc cctacgcgcg
     2761 ctgctagtag tatcgctact tccggtgagc gaaattaacc agcgcttcag gcccggctgc
     2821 ggccgggcag gaggcgggtt gcaggtccgg gcgactcgcc ggagtcctgt gcctcgcaac
     2881 tcgaagcggc gagaagatca tacggagaat gaatgactta agcaaggccg gggcccggct
     2941 caaggcaacc tgggtcgaag tcggcgagga gagcgaggcg cagcgcatag acaacttcct
     3001 gctgcgcaag ctgaagggcg tgccgaagag ccacgtctac cgcgtgctgc gcagcggcga
     3061 ggtgcgggtg aacagcggcc gcgtcaagcc cgattaccgg ctgcgcgccg gcgaccgcat
     3121 ccgggttccg cccatccggg tcgccgaagg ccagcgggca gcgaggccgc atgagttccc
     3181 ggtcgtgtac gaggacgcct ccctcctggt gatcgacaag cccgccgggg tcgcggtgca
     3241 cggcggcagc ggtgtgagct acggcgtcat cgagtcgtta cgggcggcgc ggccgcaggc
     3301 gaagctcctc gagctcgcgc accggctgga ccgcgatacc tccggcctgc tcatgctcgc
     3361 caagaagcgc agcgccctgg tggagctgca ccggatgctg cgcgaagggg aggtggagaa
     3421 gatctacctc gccgtggtca acggcgagta caaagggccg gccgagatcc gcgccgccct
     3481 gcacaagtac gtcaccggct cgggggagcg ccgggtcgcg gttaccgagg acggcaggtc
     3541 ggccctcacg cgcgtcaagg tcctgcggcg gaacgccgag tcgagcctgc tcgaagttcg
     3601 gctcctgacc ggccgcacac accagatccg cgtgcacctc gcgcacgtgg ggcatccgat
     3661 cttcggcgat gaaaagtacg gcggcggcga agcgaagcga ctgctcctgc atgcggcgaa
     3721 gctcgctttt cgccatccgc tcaccggcga gccgatgcgc ctggcatcgc ccgcgcctgc
     3781 cgaaatgaaa gccgccctgt aatgaagtac cgcctcctcg tgttcgactg ggacgggacg
     3841 atcatcgatt cggcctcgac cattgccgag tgcatccgcg aggcgagccg cagcctgggg
     3901 ctgcaggtgc ccggcgacga gcgcgcgcgc catgtgatcg gcctcgggtt gcacgacgcc
     3961 atgcggatcg tggtgccgga gctgcccgca agccgctacc cggaattcgt cgcgagctac
     4021 cgcgcccact tccttgcgcg caaggattcg atgcagcttt tcgccggcat gcgcgagctt
     4081 ctcgaggagc tttgcggcag gcatctgctc gccatcgcga cgggcaagag ccgcgcaggc
     4141 ctcgatcgcg acatcgactt ccatggcctg aaggctctct ttgcggcttc gcgctgcgcc
     4201 gacgagaccc accccaagcc gcacccggcg atgctgatgg agctcatgga cgagctcgcg
     4261 gtgccaaacg atgccgcgct catgatcggc gacacttcgc acgacctcga gatggcccgt
     4321 gccgcggacg tcggcgcgct cgccgtgagc tacggggcgc acgcggaaca tgggctgcgc
     4381 gcctgcgggc cgctcgggtg cttttccagc gtcggcgagc tcaaggcatg gctggcggcg
     4441 aacggctgat ctgcgcgggc gccgcgctcg ccgaagccgg ccgcggcgtg cgcttcgagg
     4501 tcgaatattt cggcgagccc gcgccggcgt tcgtggtgcg caagaacggt gcagtgcacg
     4561 gctacctgaa ccgctgcgcc cacgtcgcga tggagctcga ctggcaggaa ggcacgttct
     4621 tcgatgccga cggacgcgat ttgctatgct cgacgc
//
LOCUS       DASUNT010000616         4543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1042597, whole genome shotgun sequence.
ACCESSION   DASUNT010000616 DASUNT010000000
VERSION     DASUNT010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042597"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VFX94_12425"
     CDS             complement(<1..757)
                     /locus_tag="VFX94_12425"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX5769033.1"
                     /translation="MKNPLIVAGKSYSSRLLIGTGKYRDFAQTRAAVEASGAEIVTVA
                     IRRTNIGQNANEPSLLEALPPSKFTYLPNTAGCYSAEDAVRTLRLARELLDGHELVKL
                     EVLGDPHTLYPNMPETLKAAEQLVKEGFKVMVYCSDDPIQAQMLEATGCVAIMPLASL
                     IGSGMGIINPWNLQIIIDRVKVPVLVDAGIGTASDAAIAMELGCDGVLMNTAIAKARD
                     PVRMARAMKLAVEAGREAYLAGRMPKKPYEASPS"
     gene            complement(759..962)
                     /gene="thiS"
                     /locus_tag="VFX94_12430"
     CDS             complement(759..962)
                     /gene="thiS"
                     /locus_tag="VFX94_12430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX5769034.1"
                     /translation="MICVTVNGAAHRFDTAVDLSGLLSSLNLQGKKVAVERNGEIVPK
                     SVHASTLLADGDRLEIVVAVGGG"
     gene            complement(982..1218)
                     /locus_tag="VFX94_12435"
     CDS             complement(982..1218)
                     /locus_tag="VFX94_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769035.1"
                     /translation="MGVIQRFVGVLVGLLFLAAVLVFASLALGVLLAVGLAIWGWVWW
                     RTRALRRAPGRGVVIEGEYRDVTRSQRIEDRERP"
     gene            1239..1312
                     /locus_tag="VFX94_12440"
     tRNA            1239..1312
                     /locus_tag="VFX94_12440"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1271..1273,aa:Gly,seq:ccc)
     gene            1667..2572
                     /locus_tag="VFX94_12445"
     CDS             1667..2572
                     /locus_tag="VFX94_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEX5769036.1"
                     /translation="MPYSVEGKLVVAISSRALFDFEEENRVFEKDGEPAYIALQYARL
                     DVPARQGVAFALVQKLLAFNTPGQSRVEVVVLSKNDPVSGLRVFRSAKQAGLMIERGV
                     FTRGRSPYRYLDALKANLFLSANEADVMAALDAKVPAARVYPESTQAASRHADEVRIA
                     FDGDAVLFSDEAERVYQRDGLDAFTKHETARALQPLPPGPFKPLLEALHRLQTASGKE
                     VPVRIRTALVTARSAPAHERAVRTLMEWNIAVDEAMFLGGLAKGEFLKSFEPDFYFDD
                     QRGHIESARAYVAAGHVPFGVTNIR"
     gene            complement(2605..3513)
                     /locus_tag="VFX94_12450"
     CDS             complement(2605..3513)
                     /locus_tag="VFX94_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX5769037.1"
                     /translation="MQLLKLESVSRDFDVSPPWLNRVIEREPRRILRAVDHISFEISQ
                     GETLALVGESGCGKSSVARLIAGLYAPTEGRIEFHGRRLQMIFQDPYASLNPRWRVRS
                     IIAEPMRFLKEKKPAEEFLLQVGLSPDDAQRYPHEFSGGQRQRISIARALAGEPDLLI
                     CDEPTSALDVSVQAQILNLMAELQTRLALTYLFISHNLAVVSLVADRVGVMYLGRIVE
                     LGGAAQLFSRPRHPYTRMLLDAVPDLAMSGKPRTPVAGEVPNPLAPPPGCAFHPRCPH
                     ANARCQSEVPRFRDGVACHAVEEGRI"
     gene            complement(3500..4459)
                     /locus_tag="VFX94_12455"
     CDS             complement(3500..4459)
                     /locus_tag="VFX94_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5769038.1"
                     /translation="MILEVEGLTVQFPTRRGILTAVDAITFSIAPGEVLGVVGESGAG
                     KSITGLAVIRLLEPPGRIAAGEIRLEGRRIDKLPHEEMRRIRGRRIGVIFQDPLTSLN
                     PLYTIGQQLEETILTHLKLSPKAARERALALLREVGIPAPELRYNSYPHHFSGGMRQR
                     VVIALALAAEPRLLIADEPTTALDVSIQAQIITLLKRLAAERGAAVMLITHDMGVIAE
                     TAQRVAVMYAGRIVEIGPVREVIHAPRHPYTVGLMGSIPRITSHREKLVQIDGAMPRL
                     NAIPPGCAFNPRCPRRMERCTRERPELVPAGASRAACWLHAAS"
BASE COUNT          746 a         1475 c         1579 g          743 t
ORIGIN      
        1 agctcgggct cgcctcgtag ggtttcttcg gcatgcgccc ggcgaggtag gcctcgcggc
       61 cggcctccac cgcgagcttc atcgcacggg ccatgcgcac gggatcgcgc gccttggcga
      121 tggcggtgtt catgagcacg ccgtcgcaac ccagctccat cgcgatcgcc gcgtcggaag
      181 cggtgccgat gcccgcgtcg accagcaccg ggaccttcac gcggtcgatg atgatctgca
      241 ggttccacgg gttgatgatg cccatgcccg acccgatgag cgaagcgagc ggcatgatcg
      301 ccacgcagcc cgtggcttcc agcatctgcg cctggatcgg atcgtcagag cagtacacca
      361 ttaccttgaa gccctcttta accagctgct cggctgcctt cagcgtctcg ggcatgttgg
      421 ggtagagcgt gtgcggatcg cccaggacct cgagcttcac gagctcatga ccgtcgagaa
      481 gctcgcgcgc gagccgcagc gtgcgcacgg cgtcctccgc gctgtagcac ccggcggtat
      541 tcggcaggta ggtgaacttc gacggcggca gcgcctcgag caggctcggc tcgttggcgt
      601 tctggcctat gttggtacgc cggatggcta cggtcacgat ttcggcgcct gaagcctcca
      661 ccgcggcgcg cgtttgcgcg aagtcgcggt acttccccgt gccgatgagg agccgcgagg
      721 aatagctctt acccgcgacg atcagggggt tcttcacgtc acccgcctcc caccgcgacc
      781 acgatctcga gccggtcgcc gtcggcgagc agcgtggacg cgtgcacgct cttcggcacg
      841 atttcgccgt tgcgttcgac ggccactttc ttaccctgaa ggttgagcga agagagcaag
      901 cccgagaggt ccaccgccgt atcgaaacgg tgcgcggcgc cgttcaccgt gacgcagatc
      961 atgcggtaat gataccgccg gctaaggtct ttcgcggtcc tcgatgcgct gcgagcgggt
     1021 cacgtcgcgg tactcgccct cgataaccac cccgcgtccc ggagcacgcc gcagcgcgcg
     1081 cgtgcgccac cacacccatc cccagatcgc gaggcccacg gcgagcagta cgccgagcgc
     1141 tagcgaagcg aagaccagga ccgcggccag gaacagcagg ccgaccaaga cccctacgaa
     1201 ccgctggatg actcccattg gtctatgatt cgccgtccgc gggtgtagtt caatggcaga
     1261 acatcagctt cccaagctga atacgtgggt tcgattccca tcacccgctc caacgcagcg
     1321 tggcagtggt cagtcgccgg ggagagaaaa acgatccagc gttgagggtc tttaccagcg
     1381 ccgctcacag agcttggcgt aggcgaagcc agttccgtcc tgccgccgtg ctcaaggcgc
     1441 tcgccgcggc tgatcaccgt cgcaagcggc tcctcgtccg accagtatcg gaacagcgca
     1501 tgcaggcagg tggtccgcag gtgcttttca tccggctgcg gcagggtgct caccggaagg
     1561 cagaatgcac ctttcctgcg acgctccttc agcatgcgag aactgcacgg caatcgctgt
     1621 gccggcgcat tgcgcttgcg cgcataatca cgcccgaggg aggcgaatgc cctattcggt
     1681 tgaaggcaag ctggtcgtgg cgatctcgtc gcgggcgctg ttcgacttcg aagaagaaaa
     1741 ccgggtgttc gagaaggacg gcgagccggc gtacatcgcg cttcagtatg cgcggctcga
     1801 tgttccggcg cgccaggggg tggcgtttgc gctggtccag aaactgctcg cgttcaacac
     1861 cccggggcag agccgcgtcg aggtcgtggt gctatccaag aacgacccgg tttccggcct
     1921 gcgcgtgttc cgctcggcca agcaggcggg gctcatgatc gagcgcggcg tgttcacgcg
     1981 cggtcgcagc ccctaccgtt acctggatgc cctcaaggcg aatctattct tgtccgcgaa
     2041 cgaggccgac gtcatggcgg cactcgacgc gaaggtgccg gcggcccgcg tgtaccccga
     2101 gtccacccag gcagcgagcc gccacgccga cgaagtgcgc atcgcctttg atggcgacgc
     2161 ggtgctcttc tccgacgagg cggaacgcgt ctaccagcgc gatgggctgg acgcgttcac
     2221 gaagcacgag accgcgcggg cgctgcagcc gctgccgccg ggtccgttca agccgctcct
     2281 ggaggctctt caccgcctgc agacagcctc gggcaaggag gtgccggtgc gcataagaac
     2341 cgccctggtt accgcccgca gcgcgccggc gcacgagcgc gcggtgcgca cgctgatgga
     2401 gtggaacatc gcggtagacg aggcgatgtt cctcggcggc ctcgccaagg gcgagttcct
     2461 gaagtcgttc gagcccgatt tctacttcga cgaccagcgc gggcacatcg agtcggcgcg
     2521 tgcctacgtg gccgccgggc acgtgccttt tggcgtgacg aacatccgct aaccgaaaaa
     2581 ggggtcagga cccttattcg cccctcagat ccggccttcc tccaccgcgt ggcaggcgac
     2641 gccgtcgcgg aagcgcggaa cctcgctctg gcagcgggca ttggcgtggg gacagcgagg
     2701 atggaaggcg cagcccggcg gcggggcaag cgggttgggc acttcgccgg cgaccggggt
     2761 gcgcggcttg ccgctcatgg cgaggtcggg caccgcgtcg agcagcattc gcgtgtacgg
     2821 gtggcgcggc cgcgagaata gctgcgcggc gccgccgagc tcgacgatgc ggccgaggta
     2881 catcacgccg acgcgatcgg cgacgaggga caccaccgcg agattgtgcg agatgaagag
     2941 gtaggtaagc gcgaggcggg tctgcagctc tgccatgagg ttgaggatct gcgcctggac
     3001 cgagacgtcg agcgcggaag tcggttcgtc gcagatcagg agatcgggct ccccggcgag
     3061 ggcgcgcgcg atcgagatgc gctggcgctg gccgccggag aactcgtgcg ggtagcgctg
     3121 cgcgtcgtcc ggcgagaggc ccacctgcag caggaattcc tccgcaggct tcttttcctt
     3181 cagaaaccgc atcggctcgg cgatgatgct gcgcacgcgc cagcgcgggt tgaggctcgc
     3241 gtacgggtcc tggaaaatca tctgcaggcg ccggccgtgg aattcgatgc gtccctcggt
     3301 gggcgcatag agtccggcga tcaggcgtgc gacgctggat ttgccgcaac cggactcgcc
     3361 gacgagggcg agggtctccc cttgcgaaat ctcgaagctg atgtggtcca cggcgcgcag
     3421 gatgcggcgc ggctcgcgct cgatcacgcg gttgagccac ggaggcgaga cgtcgaagtc
     3481 ccggctgacg gactcgagct taagaagctg catgcagcca gcaagccgcc cggctcgccc
     3541 ccgccggcac gagctcgggc cgctcgcgcg tgcagcgttc catgcgccgc gggcagcgcg
     3601 ggttgaaggc gcatccgggg gggatggcgt tcaggcgggg cattgcaccg tcgatctgga
     3661 cgagtttttc gcggtgagaa gtaattcgtg gaatggagcc catcaggccg acggtatacg
     3721 ggtggcgcgg cgcgtggatc acctcgcgca ccgggccgat ctcgacgatt ctgccggcgt
     3781 acatcacggc gacgcgctgc gccgtctccg cgatcacgcc catgtcgtgg gtgatcagca
     3841 tgaccgcggc gccgcgctcg gcggcgaggc gcttgaggag cgtgatgatc tgcgcctgga
     3901 tcgagacatc gagcgcggtg gtcggctcgt cggcgatgag cagccgcggc tcggccgcga
     3961 gcgcgagcgc gatgacgacg cgctggcgca tgccgcccga gaagtggtgc gggtagctgt
     4021 tgtagcgcaa ttcgggcgcc gggatgccga cttcccgcag aagcgcgagc gccctttccc
     4081 gcgcggcctt cggcgagagc ttgaggtgcg tcaagatggt ctcttcgagc tgctgtccga
     4141 tggtgtagag cgggttgagc gaggtgagcg ggtcctggaa gatgacgccg atcctgcggc
     4201 cgcggatgcg gcgcatctct tcgtgcggca gcttgtcgat gcgccggcct tcgagccgga
     4261 tttcgcccgc ggcgatccgc ccgggcggct cgagcagccg gatgacagcg agcccggtga
     4321 tcgatttgcc ggcgccggat tcacccacga cgccgagcac ctcgccgggc gcgatggaga
     4381 acgtgatcgc atccaccgcg gtgaggatcc cgcgccgcgt cggaaactgc accgtgagcc
     4441 cctcgacttc gaggatcatc gaagcttcgg gttcagggcg tcgcgcagcc agtcgccgag
     4501 caggttcacc gacagcacga gcacgacgag cgccacgccc ggg
//
LOCUS       DASUNT010000617         2666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_954572, whole genome shotgun sequence.
ACCESSION   DASUNT010000617 DASUNT010000000
VERSION     DASUNT010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954572"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..2054)
                     /gene="mrdA"
                     /locus_tag="VFX94_12460"
                     /pseudo
     CDS             complement(138..2054)
                     /gene="mrdA"
                     /locus_tag="VFX94_12460"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
     gene            complement(2051..2566)
                     /gene="mreD"
                     /locus_tag="VFX94_12465"
     CDS             complement(2051..2566)
                     /gene="mreD"
                     /locus_tag="VFX94_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX5769039.1"
                     /translation="MDLSASSSRQILQPVRVSTIVASVALALFLDFLPWRDVRLMPDF
                     VALVLLFWCVRQPRLVGLGVAWTLGLVTDAGNGVLLGQHALAYSLLAFLGIWLSRRIL
                     WFGPWLQALHVGAILLLTQGVAVLVRLAAGDQFPGWQVFVGPLAGALLWPPTSWLLLL
                     PQRRAAKEQTI"
BASE COUNT          449 a          867 c          915 g          435 t
ORIGIN      
        1 gggggcatcg aagctcgcgg agaacaacgc cgcgaagccc agcatgcaga gcgacgcgac
       61 gatgacgagc agcgtggcgt cgagtccttc cacgagccgc agagcgatgc gctggacggg
      121 aacgcgggcg atctccatca atcgctttcg gttccgtcgt tgaactcgtc ctccggggtg
      181 ggctcctgca gccccggcgg cagcttgccg agcaggtaat agtcgagcac cgtgcgcgag
      241 atgggcgcgg ctgcgcgcgc accgaacccg ccattttcga cgatcacggc aagcgcgatg
      301 accgggttgt cgaccggcgc gaacgcgatg aacagcgaat ggtcgcggtg ccgctcggcg
      361 atcttcgctt cgtcgtactt ctcgccttgc ttcatgccga ccacctgcgc ggtgccggtc
      421 ttgccgccgc tcgcgtactc ggccttggca aaggcgcgtg cgccggtgcc ctccttattg
      481 accccggcca tggcgcgctt cacgaactcc acccactcgc gccgcagcgg cacctgcgag
      541 ctgagctgcg gctcgaacgt gcggcgctca cgcgtgcgcg ggttctcgat atggctcacc
      601 acgtgcggcc ggtacatgtc gccgttggcg gctaccgtgg ccatcgcgtg agcaagctgg
      661 atcggggtgt atgcgttgta gccctggccg atgccgatgg aaatggtcta ccccgggtac
      721 cagcgctgct gctccgggcg gcggaagcgc ttcgttttcc actccggcga gggcagcacg
      781 ccggccgcct cgccttgcag gtcgatcccg gtcgggctgc cgagcccgag cagcttcatg
      841 aagccggcga tcgcgtcgat tcccatgtcg ttcgccagca tgtagtaata cgtgtcggag
      901 gacacgacga tggacttgta gaggtctacc acgccatgcc caccgggctt ggagtcgcgg
      961 aagcgtcggt ccccgaatac gaaatagccc ggatcgttga tggtgctcga cgggcggcgc
     1021 ttgccgagct cgagcgcggc gagcgccatg aacggcttga acgtggaccc gggcgggtag
     1081 accccggcga tggcgcggtt gttgagtggc ctgtcggggg agtggttgag ctcggtccag
     1141 gcctgcgggt cgataccgtc gacgaaaagg ttcaagtcga agcccggctt ggacacgagc
     1201 gcgagcaccg cgccgctgcc gggctcgatc gcgaccagcg cgccccggcg gttgccgaac
     1261 gcgttctccg caacgcgctg cagcagcatg tcgattgtca gagcgacgtt ctgcccggac
     1321 accgcggggg tctgcgaaag cgtgcggatg ccgcgacctg cggcatcgat ctcgacctgc
     1381 tcgaagcccg ttgtgccatg gagctcgcgc tcgtagcttg cctccacgcc gctctttccg
     1441 atgtagtcgc tgccacggta gttggcatct acgccttctt gctcgagggc cgcctggtcg
     1501 ccctggttga tgcggcccat gtaaccgatg acatgcgagc cgacgtcgcc gaaggggtac
     1561 tggcggaaaa gccgcgcctt gatcgcgacg ccttcatagc ggaagcggtt cgccgcgaac
     1621 ttcgccacct cctcgtcgga aaggcgggtc cggatgggaa ggctctcggg attgcgcgtc
     1681 tccaccgcga ggcgctttaa acgcgccctg tccctgggcg tgatctcgat cagctcggac
     1741 acctggtcga tcgtctcctc gacgctcttt acccggcgcg ggaagatctc gagcgtgtag
     1801 gcggaatagt tgcgcgccag gacaacgccg ttgcggtcgg tgatgaggcc gcggttcggc
     1861 ggaatgggaa cgatggcgat gcggttgtcc tcggctttcg cgcggtagtg gtcatgctgc
     1921 accacctgca ggaaaaagaa gcgcgcgaga aggatgccaa aggcggcgag catggcgatt
     1981 gcggcgatgc cgatgcgcag ctggaactgc tgcagctcgc gctcggagtt gcgcagctca
     2041 gccgcgccca tcatatggtc tgctccttcg ccgctcgccg ctgcggtagc agcagcagcc
     2101 agctcgtcgg cggccaaagc agcgcgcccg cgagcgggcc gacgaacacc tgccagccgg
     2161 gaaattgatc gcccgcggcg aggcgcacga gcaccgcaac cccctgcgtc aggagcagga
     2221 tcgccccgac atgcagcgcc tgcagccagg ggccgaacca gaggatgcgg cgggacagcc
     2281 agatgccaag aaaggcgagc agcgaatagg cgagggcgtg ctgtccaagc agcacgccgt
     2341 tgcccgcgtc ggtcactagg ccgagagtcc aggcgacgcc gaggcccacc agccgcggct
     2401 ggcgcacgca ccagaagagc agcacgagcg cgacgaaatc gggcatcagg cgcacgtcgc
     2461 gccaaggcag gaagtcgaga aaaagcgcga gcgcgaccga cgcgacgatg gtactcacac
     2521 gcaccggctg caggatctgg cggctcgagc tagcggagag atccatcctt gtccttcagc
     2581 cggccgcggc gcgtgcgctc cttcgtgctc tgcgcttcct cgggtcgcgc gggcttgatc
     2641 gtctcgtccg acagcacgag aacgaa
//
LOCUS       DASUNT010000618         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_254757, whole genome shotgun sequence.
ACCESSION   DASUNT010000618 DASUNT010000000
VERSION     DASUNT010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254757"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VFX94_12470"
     CDS             <1..652
                     /locus_tag="VFX94_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination factor protein RarA"
                     /protein_id="HEX5769040.1"
                     /translation="DAAETREAAVDAAAVQRVAQRKVLVYDKSGEEHFNLISALHKSL
                     RESDPDAGLYWFARMLEAGEEPGYLARRLTRFAVEDVGLADPRALRVTLDACETYERL
                     GSPEGELALAEAALYLAVAAKSNAVYEAYNAARDFVSADGTRPVPLHIRNAPTPLMKN
                     LGHGKGYRYAHDEPDAYSAGQTYFPEGMPAVEWYRPTERGLEAKIRQRIEELRSKK"
     gene            649..1926
                     /gene="serS"
                     /locus_tag="VFX94_12475"
     CDS             649..1926
                     /gene="serS"
                     /locus_tag="VFX94_12475"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX5769041.1"
                     /translation="MIDIQLLRRNPEEVAKRLATRGPGAFDVAQFERIEAARKDLQTA
                     VEQAQASRNRIAKDIGQAKAKGHDVAPLLEQAEKLKGLLEKSEQQLALLQQEMQEFLR
                     RVPNIPHESVPLGASSQESREERRWGSPREFSFSPRDHVDVGEALGGLDFATAAKVAG
                     SRFVAMRGEVAHLHRAIAQFMLDVHTREHGYTEVYAPYMVSAECADGVSSLAKFKGDL
                     FKIEGRELYLIPTAEYPVTNFVREEIVELKSLPLKLVCHSPCFRSEAGSAGKDTRGMI
                     RNHQFDKVEILQVTHPAQSYQTLEELTGHAETILKRLELPYRVVSLCTGDIGFASAKT
                     YDLEVWLPAQNTYREISSCSNFEAFQARRMQARFRNENGKPELVHTLNGSGLAVGRTL
                     VAILENYQNADRSVTVPTALRNYMDGAERLAPA"
     gene            1962..2051
                     /locus_tag="VFX94_12480"
     tRNA            1962..2051
                     /locus_tag="VFX94_12480"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1996..1998,aa:Ser,seq:cga)
BASE COUNT          408 a          699 c          679 g          335 t
ORIGIN      
        1 ggacgccgcg gagacccgcg aggccgcggt cgatgccgcg gcggtccagc gcgtggcgca
       61 gcgcaaggtg ctcgtctacg acaagtccgg cgaggaacac ttcaacctga tctcggcgct
      121 gcacaagtcg ctgcgcgaga gcgatcccga cgccggtctc tactggttcg cgcgcatgct
      181 cgaagccgga gaggagcccg gctacctggc gcgccgactg acccggtttg ccgtcgagga
      241 cgtcggcctc gccgatccgc gcgcgctgcg cgtgacgctg gatgcctgcg agacctacga
      301 gcgcctcggc agcccggagg gcgagctcgc cctcgccgag gcggcgctct acctcgcggt
      361 ggcggcgaaa tccaatgcgg tgtacgaagc ctataatgcc gcgcgcgact tcgtctcggc
      421 cgacggcacg cgaccggtgc cgctccacat ccggaacgcg cctacgccac tcatgaagaa
      481 cctcgggcac ggcaagggct accgctacgc gcacgacgag cccgacgcct attccgcggg
      541 gcagacttat ttccccgagg gcatgcctgc cgtcgagtgg tacaggccga ccgagcgcgg
      601 gctcgaagcg aagatcaggc agagaatcga agagctgaga agcaaaaagt gatcgacatc
      661 cagctgctac gcaggaatcc cgaggaagtc gcgaaacgcc tcgccacgcg cggcccgggg
      721 gcgttcgacg tcgcgcagtt cgagcgcatc gaggcagcgc gcaaggacct gcagaccgcg
      781 gtcgagcagg cgcaggcttc gcgcaaccgc attgcgaagg acatcggcca ggcgaaggcg
      841 aagggccacg atgtcgcccc gctcctcgag caggcggaga agctgaaagg gctgctcgag
      901 aaatcggagc agcagctcgc ccttctccag caggagatgc aggagttcct gcgccgcgtg
      961 cccaacatcc cgcacgagtc cgtgccgctc ggcgcatcgt cgcaagagag ccgggaagag
     1021 cgtcgctggg gaagccccag ggagttttcc ttttctccaa gagatcatgt ggacgtcggc
     1081 gaagcgctcg gcggactcga cttcgccacc gcggccaagg tcgcgggcag ccgcttcgtc
     1141 gcgatgcgcg gagaggtggc gcacttgcat cgcgccattg cgcaattcat gctcgatgtg
     1201 cacacgcgcg agcacggcta caccgaggtg tacgcgccgt acatggtgag cgcggaatgc
     1261 gccgatggcg tctcgagcct ggcgaagttc aaaggcgatc tcttcaagat cgaaggacgc
     1321 gagctctatc tcatcccgac ggcggaatac ccggtaacga acttcgtgcg cgaggaaatc
     1381 gtcgagctga aaagcctgcc gctcaagttg gtctgccact ctccctgctt ccgctcggaa
     1441 gcgggctcgg cgggcaagga cacgcgcggc atgatccgca accatcagtt cgacaaagtc
     1501 gagatactcc aggtgacgca tcctgcgcag tcctaccaaa cgctggagga gctcaccggg
     1561 cacgccgaga cgattctgaa gaggctcgag ctaccgtatc gagtcgtctc cttgtgcacc
     1621 ggcgatatcg gctttgcgag cgcgaagacc tacgatctcg aggtatggct ccccgcccag
     1681 aacacctacc gcgagatctc ttcctgctcc aacttcgagg ccttccaggc gcggcgcatg
     1741 caggcgcgct ttcgcaacga gaacggcaag cccgagctcg tgcacacgct gaacggatcg
     1801 ggtctcgcgg tcgggcgcac gctcgtcgcg atcctcgaga actatcagaa cgccgaccgg
     1861 tctgtcaccg tgccgaccgc gctacgcaac tacatggacg gagccgagcg cctcgctccc
     1921 gcctgataaa atccgcggct cgcgcttttc gttcgtaccg cggagagatg ccggagtggt
     1981 cgaacggggc agactcgaaa tctgttgtac ggcttgccgt accgggggtt cgaatccctc
     2041 tctctccgcc aactttcaac agcttacaaa gccacggctt cttccttcgc ttcggcacgg
     2101 tgacgtaaat gtgccgtagc t
//
LOCUS       DASUNT010000619         3853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_780206, whole genome shotgun sequence.
ACCESSION   DASUNT010000619 DASUNT010000000
VERSION     DASUNT010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780206"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VFX94_12485"
     CDS             <1..777
                     /locus_tag="VFX94_12485"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX5769042.1"
                     /translation="ASLRDLRAFLARRGCQVLAEGETEADLAIVIGGDGTMLSAAREL
                     VRHRVPLVGVNQGRLGFMTDIGHQDMQRGVGAILDGEYTLEERSLLDCEIRRDGNSLV
                     RTIALNDAVLGKGAEGRLIEFQLSVDGEFVYTLRADGVIVSTPTGSTAYALSAQGPIL
                     HPAVPALTLVPLNPHTLSARPVSVSDRSVIEISIVHALDARAHFDGLALADMAEGDRL
                     AIRRSDDVVRFVHPPGYRYFATLREKLGWSAAIDRRRGGG"
     gene            780..2417
                     /gene="recN"
                     /locus_tag="VFX94_12490"
     CDS             780..2417
                     /gene="recN"
                     /locus_tag="VFX94_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX5769043.1"
                     /translation="MLRALEAANFVIIEQTSVEFGPGFTALTGETGAGKSILVDAIEL
                     LVGGRGDATLVREGAERAELSAEFDSEEKTLSSWLDESGLAGDPGTVLLRRTIDRAGR
                     SRCFINGHAVTLAQLKAAGEHLVDIHGQHEHQSLLRAAAQRALLDSHSSSEEVAGQTA
                     RSYRAWKRLQELADEAQKSFAQREREREDVQSLVSELKTLNPREGEWEQVSAEHTRLQ
                     HGSSLLGGAQSALEALAEAEGACLGQLAAVASRLKALSQHDARLDTVVEMLDSAEAQA
                     AEAVRTLRQYASRVDLDPEALREVEARIEALHAAARKHRVRPHALHELLRDSEKRLNE
                     LELAIDPEALRREVAAAKADFDSLCRKLGSLRRAGAEALGKAVTAAMQQLAMAGGRFS
                     ASLPTLKEPGSSGGEEVEFEVCSHPSLPLRPLAKVASGGELSRVSLAIQLVAAKASPV
                     GTLVFDEIDAGIGGAVAETIGRSLRRLGSQRQVLCVTHLPQVAASANDQWSVSRSGLT
                     VKVQRLGRAARIAELARMLGGAESTARKHAAELLDAT"
     gene            complement(2491..2916)
                     /gene="fur"
                     /locus_tag="VFX94_12495"
     CDS             complement(2491..2916)
                     /gene="fur"
                     /locus_tag="VFX94_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEX5769044.1"
                     /translation="MSTVQSLKDSGLKATLPRRKILELFESSKVRHLSAEDVYKALLA
                     EGLDVGLATVYRVLTQFEHAGLLARQHFETGKAVFELNQGGHHDHLLCLQCGRVEEFY
                     DAEIEKRQTEIARARGFALHGHSLALYADCTKQDCPYHP"
     gene            2936..3298
                     /gene="bamE"
                     /locus_tag="VFX94_12500"
     CDS             2936..3298
                     /gene="bamE"
                     /locus_tag="VFX94_12500"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEX5769045.1"
                     /translation="MPRRPFALLLSAALSLAVSGGCSSVPRVPGITPYRIDIQQGNYI
                     SSDMVAQLKPGMSKEQVRLALGTPLLTDIFHADRWDYVYWYERAGNKPEQRKLTVFFE
                     DGKLTRLDGDVVAAGATQ"
     gene            3295..>3853
                     /gene="dapB"
                     /locus_tag="VFX94_12505"
     CDS             3295..>3853
                     /gene="dapB"
                     /locus_tag="VFX94_12505"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX5769046.1"
                     /translation="MRIAIAGAGGRMGRTVIEAVSADPEIELVAAVDMPGSPALGQPA
                     GKIRIVCALETLARADVVLDFTRPEGTLAFLAHARAMVIGTTGFTEEDKDRIAQAAKR
                     IPIVQAANFAVGVNIAYKLAETAARILGEDYDVEIVEAHHRHKVDAPSGTALAMGETV
                     AAALGRDLKQCAVHGREGVTGERDPS"
BASE COUNT          609 a         1294 c         1352 g          598 t
ORIGIN      
        1 gcctcgctgc gcgatctgcg cgcgttcctc gcgaggcgcg gctgccaggt tcttgcggag
       61 ggcgaaaccg aagccgatct cgcgatcgtt atcggcggcg acggcacgat gctctccgcg
      121 gcgcgcgagc tcgtgcgcca ccgagttccg ctcgtcggcg tgaaccaggg ccggctcggc
      181 ttcatgaccg acatcgggca ccaggacatg cagcgcggcg tgggcgcgat tctcgacggc
      241 gaatacacgc tcgaggagcg ctcgcttctc gactgcgaga tccgccgcga cggcaactcg
      301 ctcgtgcgca cgatcgcgct caacgatgcg gtgctcggca aaggcgccga aggccgcctg
      361 atcgagttcc agctctcggt ggacggcgaa ttcgtctata cgctgcgcgc cgacggcgtg
      421 atcgtgtcaa cgccgaccgg ctcgaccgcg tatgcgctgt ccgcgcaggg cccgatcctt
      481 catcccgccg tgccggcgct cacgctggtg ccgctcaacc cgcacacgct ctcggcgcgg
      541 ccggtgagcg tgagcgaccg cagcgtgatc gagatctcga tcgtgcacgc gctcgacgcg
      601 cgcgcgcatt tcgacggcct cgcgctcgcg gacatggccg agggggaccg cctcgcgatc
      661 cggcgctcgg acgacgtggt gcgcttcgtg cacccgccgg gctaccgcta cttcgccacg
      721 ctgcgcgaga agctcggctg gagcgcggcg atcgacaggc gccgcggcgg gggttgacgg
      781 tgctgcgcgc gctcgaagcg gcgaatttcg tcatcatcga gcaaacgtca gtggagttcg
      841 gtcccggctt cacggcgttg accggcgaga ccggcgccgg caagtcgatc ctcgtcgacg
      901 cgatcgagct cctcgtcggc ggccgcggcg acgcaaccct ggtgcgcgag ggtgcagagc
      961 gcgcggagct ttcggcagaa tttgattccg aagagaagac attgtcctca tggcttgatg
     1021 aatccggtct cgcgggggat cccggcaccg tgctgctgcg aaggaccatc gaccgcgcgg
     1081 ggcgctcgcg ctgcttcatc aacggccacg ccgtcacgct cgcccagctc aaggcggcag
     1141 gcgagcatct tgtcgacatc cacggccagc acgagcacca gtcgctcctg cgcgcggccg
     1201 cgcagcgggc acttctcgac tcgcattctt cttcggaaga agtcgccggg cagactgcgc
     1261 ggtcgtatcg cgcttggaag cggctccagg agctcgcaga cgaggcccag aagagctttg
     1321 cgcagcgcga gcgcgagcgc gaggacgttc agtctctcgt ttccgagctg aagacattga
     1381 acccccggga gggcgagtgg gagcaggtct cggccgagca cacccggctg cagcacggct
     1441 cgagcctcct cggcggcgcg cagtcggcgc tcgaggcgct cgcggaagcg gaaggggcgt
     1501 gccttggcca gctcgctgcg gtggcgagcc ggctgaaggc gctgtcgcag catgacgctc
     1561 ggctcgacac cgtggtggag atgctggact ccgccgaggc gcaggcggcc gaagcggtgc
     1621 gcacgttgcg ccagtacgcc tcccgcgtgg acctcgaccc ggaagcgctg cgcgaggtgg
     1681 aggcgcgcat cgaggcgctg cacgcggccg cgcgcaagca ccgggttcgc ccgcacgcgc
     1741 tgcacgagct gctgcgcgac tcggaaaagc ggctgaacga gctcgagctc gcgatcgacc
     1801 ccgaagcgct gcgccgcgag gtggccgcgg cgaaggccga ttttgattcc ctttgccgca
     1861 agctcggctc cttgcgccgc gcaggcgccg aggcgctcgg caaggcggtc acggcggcga
     1921 tgcagcagct cgcgatggca ggcgggcgct tttccgcttc tcttcctacg ttaaaagagc
     1981 ctggcagctc tggcggggaa gaagtggagt ttgaagtttg ctcgcacccg agcctgccgc
     2041 tccgcccgct cgcgaaggtc gcctccggcg gcgagctttc gcgcgtgagc ctcgcgatcc
     2101 agctcgtcgc cgccaaagcc tcgcccgtcg gcacgctggt cttcgacgaa atcgacgctg
     2161 gtatcggcgg ggcggtcgcg gaaacaatcg gaagatccct gcgccggctc gggagccagc
     2221 gccaggtgct gtgcgtcacc cacctgccgc aggttgccgc cagcgcaaac gatcagtggt
     2281 cggtcagccg ctcagggttg acggtcaagg tgcagaggct gggtcgcgcg gcgaggattg
     2341 cggagctcgc gcgcatgctg ggcggcgccg agagcaccgc tcgcaagcac gccgccgagc
     2401 tgctggacgc tacctgaagc gcaaaagggg tcgggaacct ttttgcaggg agctacgcgc
     2461 agctgcgcaa aaaaggtttc tgaccccctt ttaggggtgg tacgggcagt cctgcttggt
     2521 gcagtcggca tagagggcga gcgagtgccc gtgcagcgcg aagccgcggg cgcgcgcgat
     2581 ctcggtctgg cgcttctcga tctcggcgtc gtagaactcc tcgacccgcc cgcattggag
     2641 gcagagcagg tggtcgtggt ggccgccctg gttgagctcg aacaccgcct tgccggtctc
     2701 gaagtgctgg cgcgcgagga gcccggcatg ctcgaactgg gtgagcacgc ggtagacggt
     2761 ggcgagcccg acgtcgagtc cctcggcgag gagcgccttg tagacgtcct cggcggagag
     2821 gtggcgcacc ttgctcgatt cgaagagctc gaggatcttg cgccgcggaa gcgttgcctt
     2881 gaggccgctg tccttcaggc tttgaaccgt gctcatcagt tatgattata tgcggatgcc
     2941 acgccgcccc ttcgcgctgc tgttgtccgc ggcgctttcg ctcgccgtgt cgggcggttg
     3001 ttcctcggtg ccgcgcgtcc cgggcatcac gccttatcgc atcgacatcc agcagggcaa
     3061 ctacatctcc tccgacatgg tggcccagct caagcccggc atgagcaagg agcaggtacg
     3121 cctcgcgctc ggcacgccgc ttctcaccga catcttccac gccgaccgct gggactacgt
     3181 ctactggtac gaaagggccg gcaacaaacc ggagcagcgc aagctcaccg tgttcttcga
     3241 agacggcaag ctgacgcgcc tcgacggtga cgtggtcgcg gccggcgcaa cgcagtgaga
     3301 atcgccatcg ccggcgcggg cggccgcatg ggacgcacgg tgatcgaggc ggtgtccgcg
     3361 gacccggaga tcgagctcgt cgccgcagtc gacatgccgg gaagcccggc cctcgggcag
     3421 cccgccggca agataaggat cgtctgcgcg ctcgagacgc ttgcccgggc ggacgtggtc
     3481 ctcgacttca cccgcccgga agggacgctc gcgtttctcg cgcacgcaag agccatggtc
     3541 atcggcacca ccggctttac cgaagaggac aaggaccgca tcgcgcaggc cgcgaagcgc
     3601 atcccgatcg tgcaggcggc caacttcgcg gtcggcgtaa acatcgccta caagctcgcc
     3661 gaaaccgcgg ctcgcatcct cggcgaggac tacgacgtcg aaatcgtcga ggcgcaccac
     3721 cgccacaagg tcgacgcgcc gagcggcacg gcgctggcga tgggcgagac cgtcgcggcc
     3781 gcgctcgggc gcgacctgaa gcaatgcgcg gtccatggcc gcgagggcgt gacgggggag
     3841 cgcgatccgt cca
//
LOCUS       DASUNT010000620         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_430778, whole genome shotgun sequence.
ACCESSION   DASUNT010000620 DASUNT010000000
VERSION     DASUNT010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430778"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2144
                     /gene="purL"
                     /locus_tag="VFX94_12510"
                     /gene_synonym="purI"
     CDS             <1..>2144
                     /gene="purL"
                     /locus_tag="VFX94_12510"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEX5769047.1"
                     /translation="LGTISPWSSKATDIAHNCGLASVRRIERGVLFHVDSSRPGIVPL
                     LHDRMTQTVLRSEDEAGKLFERVPPRPLQYIRISELREANRTLGLALSEDEIEYLDRA
                     YRAMGRDPTDTELTMFAQANSEHCRHKIFNADWIVDGERQPHSLFAMIRQTHALHPQG
                     TVVAYSDNAAVLEGRAARRFHAGADFVYRAHEGVVHTVIKCETHNHPTAIAPFPGAAT
                     GAGGEIRDEGSTGRGAKPKAGLVGFSVSHLRIPGYERPWEHAGAGRPARIASALDIML
                     EGPIGAAAFNNEFGRPNLCGYFRTFESGSRGYHKPIMLAGGLGNIGAAHAAKQPLPAG
                     TLLLQLGGPGLAIGMGGGAASSMGAGANTEDLDFDSVQRDNAEMQRRAQEVIDRCWQR
                     GPGNPIMSIHDVGAGGLSNALPELAHAAGRGARLELRAIPSEDSGMSPREIWCNEAQE
                     RYVLAVSPGNLALFSALCERERCPFAVLGTATGDGRLLVTDALLEKPPVDVELAMILG
                     KPPKLLRDVKRLPAARTPLSLADITVAAAASRVLQHPAVAAKSFLVSIGDRTVGGLCA
                     RDPFVGPWQVPVADCAVTLLDYEGYAGEAFAIGERTPLALIDAPASGRMAVGEALTNL
                     AAALPELARVKLSANWMAAAGAPGEDAALFDTVKAVALGLCPALGISIPVGKDSLSMR
                     TAWDAKEILAPLSLVVSAFAPVDDARAALTPQ"
BASE COUNT          321 a          758 c          740 g          325 t
ORIGIN      
        1 cctgggcacg atctcgccct ggtcgtcgaa ggcgaccgac attgcgcata actgcgggct
       61 cgcatcggtc cggcgcatcg agcgcggggt gctcttccat gtcgattcat cgcgtcctgg
      121 gatcgtcccc ctgctgcacg accgcatgac gcagacggtg ctgcgctcgg aggacgaggc
      181 cgggaagctc ttcgagcgcg tgccgccccg gccgctgcaa tacatacgca tctccgagct
      241 gagggaagcg aaccgcacgc tcgggctcgc cctgagcgag gacgagatcg aataccttga
      301 ccgcgcctac cgcgcgatgg ggcgcgatcc gaccgacacc gagctcacca tgttcgcgca
      361 ggcgaactcg gagcattgcc gccacaagat attcaatgcc gactggatcg tggacggcga
      421 gcgccagccg cattcgctct tcgccatgat ccggcagacg cacgcgctcc atccccaggg
      481 cacggtggtg gcgtactcgg acaacgccgc agtcctcgaa ggaagagccg cgcgcaggtt
      541 tcatgcgggc gctgacttcg tgtaccgcgc ccatgagggc gttgtgcata ccgtgatcaa
      601 gtgcgagacg cacaatcacc cgaccgctat cgcgcccttt ccgggcgcgg ctaccggcgc
      661 gggcggcgag atccgcgacg aaggctcgac cggccgcggg gccaagccga aagccggact
      721 ggtggggttt tcggtctcgc acttgcgcat tcccgggtat gagcgtccct gggagcatgc
      781 cggcgcgggc aggccggcgc gcatcgcctc ggcgctcgac atcatgctcg agggcccgat
      841 cggcgcggcg gccttcaaca acgagttcgg ccgcccgaac ctgtgcggct atttcaggac
      901 gttcgagtcc ggcagccgcg gctatcacaa gccgatcatg ctggcgggcg gccttggcaa
      961 catcggcgcc gcgcacgccg ccaagcagcc gctgcctgcc ggaacgcttt tgctccagct
     1021 cggaggtccc gggcttgcca tcggaatggg cgggggcgcc gcctcgtcga tgggcgcggg
     1081 cgcgaacacc gaggacctcg acttcgactc ggtccagcgc gacaacgccg aaatgcaacg
     1141 ccgggcgcag gaagtgatcg accgttgctg gcaacgcggg ccgggcaacc cgattatgtc
     1201 gatccacgac gtcggcgcgg gcggcctgtc gaacgcgctt cccgagcttg cgcatgccgc
     1261 cgggcgcggc gcgcgcctgg aactcagggc gatcccctcc gaggacagcg gcatgtcgcc
     1321 gcgcgaaatc tggtgcaacg aggcgcagga gcgatacgtg ctcgcggttt ccccgggaaa
     1381 ccttgcgctc ttcagcgcgc tctgcgagcg cgagcgctgc ccgttcgccg tcctcggcac
     1441 cgcgaccggc gacggccggc tgctcgtcac cgacgcgctg ctcgagaagc cgccggtcga
     1501 tgtcgagctc gcgatgattc tcggcaagcc gccgaagctg ctgcgcgacg tgaagcggct
     1561 gcctgcggcg cgcaccccgc tatcgctcgc cgacattacc gttgcggcgg cggcgagccg
     1621 cgtgctgcag catcccgcgg tcgcggcgaa gagcttcctc gtctcgatcg gcgaccgcac
     1681 cgtcggcggg ctttgcgcac gcgacccgtt cgtgggcccc tggcaggtac cggtggccga
     1741 ctgcgcggta acgctcctcg actacgaggg ctacgccggc gaagcgttcg cgatcggcga
     1801 gcgcacgccg ctcgcgctga tcgacgcgcc ggcttccggg cgcatggcgg tgggagaggc
     1861 gctcacgaac ctcgctgcgg cgctgcccga gctcgcgcga gtgaagctct ccgccaactg
     1921 gatggccgcc gccggcgcgc ccggcgagga cgcggcgctc ttcgacacgg tcaaggcggt
     1981 ggcgctcggg ctttgcccgg cgctcggcat ctcgatcccg gtcggcaagg attcgctctc
     2041 gatgcgcacc gcgtgggacg caaaagagat cctcgcgccg ctttcgctcg tggtctcggc
     2101 cttcgcgccg gtggacgatg cgcgcgccgc gctcacgccg caac
//
LOCUS       DASUNT010000621         7270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1042840, whole genome shotgun sequence.
ACCESSION   DASUNT010000621 DASUNT010000000
VERSION     DASUNT010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7270
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042840"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /gene="glnS"
                     /locus_tag="VFX94_12515"
     CDS             complement(<1..1364)
                     /gene="glnS"
                     /locus_tag="VFX94_12515"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HEX5769048.1"
                     /translation="MSKPARAPRAPSTSNFVRQVIEADLAARRYASVRTRFPPEPNGY
                     LHFGHVKSICLNFGIAAEYRGNCNLRFDDTNPEKEEQEYVDSIKEGVKWLGFDWGKKE
                     LYASDYFDQLYRFAEALIERGVAYVDSQSAEEMAAARGTFTEPGRESPWRNRSVEENL
                     KLFREMRDGKYRDGEHVLRAKIDMRSPNINMRDPAMYRIRHATHHRTADKWCIYPTYD
                     WAHGLSDCIEGISHSLCTLEFEDHRPLYDWFNQRVADAGLIQPPLSRQIEFARLNLTF
                     VVLSKRRLIQLVEQKVVDGWDDPRMPTLAGARRRGYTPEGFRIFAERIGVSKAHQVID
                     FAILEDCMREHLNEVAPRRMAVLDPLKLVIDDYPEGKEERMEVPNHPQKPDWGKRAVP
                     FSRELWIEREDYADSPPKGFFRLFPGNEVRLRYGYVVKCTAMKDGVVHCTHYPDSRSG
                     SPGA"
     gene            complement(1494..1673)
                     /locus_tag="VFX94_12520"
     CDS             complement(1494..1673)
                     /locus_tag="VFX94_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769049.1"
                     /translation="MSGRCVPLAAVPLPLVPALRLATWLPLGLPVDVLAAGLALDDGV
                     PALVLLSCDSVVAQP"
     gene            1684..1998
                     /gene="pal"
                     /locus_tag="VFX94_12525"
     CDS             1684..1998
                     /gene="pal"
                     /locus_tag="VFX94_12525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX5769050.1"
                     /translation="MYYEFDKYDVKPEYRALVESHARWLKANPQAKIVIEGNADERGS
                     REYNVALGQRRAESVTKMLTLLGAKPDQIEAISWGEEKPRAGGHDDASWAENRRADFA
                     SR"
     gene            complement(2095..3048)
                     /locus_tag="VFX94_12530"
     CDS             complement(2095..3048)
                     /locus_tag="VFX94_12530"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HEX5769051.1"
                     /translation="MRAAFVIASAFAATQAFAQAGFPPPPPKVSPAAGVIDMHVHSHP
                     DVFGRNMDDIDVAQLAKSKGMRGILLKNHVAETASRAALVMKVVPGIEVWGGIVLNKA
                     VGGVNPDAVEWMHRTYGGRGKVVWLPTFESDKHVKTLAKPDARGLVVAPGGQVTPEME
                     AILKIIARENLVLATGHVHPEEIIAVVRRGRDLGVKSMLITHGFTNVPGLTMAQAKQV
                     AEMGAVIEVCFLQFLAGPNAPLPFLTHWTQINAKSVAQAVKEIGAKSLVVSSDLGQSG
                     NMTHPDGLEAAIAAMKREGISDADIDLMMRKNPARLLGLAD"
     gene            complement(3088..3225)
                     /locus_tag="VFX94_12535"
     CDS             complement(3088..3225)
                     /locus_tag="VFX94_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769052.1"
                     /translation="MQVLPRTFTAPAPAAKDASLVAMERMGIVMDATATFEGGEWDFR
                     A"
     gene            3287..5752
                     /locus_tag="VFX94_12540"
     CDS             3287..5752
                     /locus_tag="VFX94_12540"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5769053.1"
                     /translation="MIRVLLLFAARRARGRMALSAAGIALGVALGYGVHLVNRAAVSD
                     VTAAVRAIAGEADLEVRGGRSGFPEALYPNIARLPGVSVASPALELDAGLPGTERILR
                     LIGIDILRAAMLQPQLVLEDRYELLAPDRIVLSPVAAQALGVAKGDTVSLVGAGGTVQ
                     LQVAAVVASLRGVSALTDISTAQWRFGRLGILNRIDIRLSTGTDREQAMAAIRDLLPP
                     GAHVVAVESLEEASAYPSRSYRVNLNVLAMVALFTGGFLVFSAQALEAARRRSEHALL
                     RVLGTKRAGISRLVLMEAAMLGAIGAAVGLALGYAFAHVAVRAAGADLGAGMFQGASA
                     QVKLSGAGAAVYFVAGILVALLGALVPARDAARTPAARALKAGDEQALFERVAPVWPG
                     LLLIAIGAALTQAGPVGGLPVFGYAAIACLLVGAIALVPRIAELVFRYLPLPSYPPLR
                     LALGQLRAAPGQAAVSLAAIVASFSLMAAMAIMVASFRESVDEWLARVLPAEVYFRTT
                     HSGDTALLDAAFEAGVRALPQVARADFLRSGRIVLDPARPALVLIARDRAEATFPLLE
                     RYERRAGDPPSVWISEAVVDLYGFAPGARLELPIANKSHPVLVAGVFRDYARQHGTVL
                     IDRADYIALTGDRTANDGALWLAPGATPAEAIAALRALPGAEQLDIAEPGEIREVSLR
                     IFDRSFAVTYAMEAVAVVVGLFGLSSSLGAIVLARRREFGVLRHLGLTRGQIRGMLAT
                     EGALLALVGSVAGLAAGAAISLVLVYVVNRQSFHWSMELHPPYLLLAGFIVLLVALAV
                     LTALVAGREAMGMGPVRAVREDW"
     gene            5755..6735
                     /locus_tag="VFX94_12545"
     CDS             5755..6735
                     /locus_tag="VFX94_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEX5769054.1"
                     /translation="MRRRTFLALSLLPFPALGAKVDYPAVQPNTRLQFPRDHGAHPEF
                     RIEWWYVTGWLDGPLGFQITFFRARPEEASDNPSAFNPRQILFAHAALSDPKRGRLVH
                     DQRAARAGFSLAHAELERTGVWIDDWRLELHGNRYEAHIAARDFAFDFHLLAHQLVLQ
                     GEQGFSRKGHRPNEASYYYSRPQLNLVGKLNGRDIEGTAWLDHEWSSAYMAPEAAGWD
                     WCGINFSDGGSLMAFRMRDRNGGVHFTSPGSTFRALRTWTSPRTGVQYPVEMAVNDLV
                     LKPLMDDQELDSRASTGTIYWEGAVTAHRNTNPVGRGYLELTGYWRPMKL"
     gene            complement(6704..>7270)
                     /locus_tag="VFX94_12550"
     CDS             complement(6704..>7270)
                     /locus_tag="VFX94_12550"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5769055.1"
                     /translation="LVMAAPLGERIGPRQWTGVAISFCGVYLIATQGRLAVPSFNVGD
                     MMALASMMMWAGYTVLLRVRRDPLNTIELLVMVCVFGVVFMLPWVGWELVHNPRVELT
                     LPGALAVLYSAIGSLLLAYAGWSYVVRRLGAARAGVTMHLMPAMGVALAAVFLGEHPS
                     WFHFAGIALILAGVALSSFRASSASSSR"
BASE COUNT         1122 a         2433 c         2479 g         1236 t
ORIGIN      
        1 tccgcgccgg gtgagccgga gcgcgagtcc gggtagtgcg tgcagtgcac tacgccatcc
       61 ttcatggcgg tgcacttcac cacgtagcca tagcgcaacc gcacctcgtt gccgggaaaa
      121 agcctgaaga agcccttggg cggagaatcc gcgtagtcct cgcgctctat ccagagctcc
      181 ctcgagaagg gaacggcgcg cttgccccag tcgggctttt gcggatggtt cgggacctcc
      241 atgcgctcct ccttgccctc gggatagtcg tcgatcacga gcttcagcgg gtcgagcacc
      301 gccatgcgcc gcggggccac ctcgttcagg tgctcgcgca tgcagtcctc caggatcgca
      361 aagtcgatga cctggtgcgc cttggacacg ccgatgcgct cggcaaagat gcggaaccct
      421 tcgggtgtgt agccgcggcg gcgcgcgccg gcgagcgtgg gcatgcgcgg gtcgtcccag
      481 ccgtccacga ccttctgctc cacgagctgg atcagccgcc gcttcgagag cacgacaaac
      541 gtcaggttca accgcgcgaa ttcgatctgc ctggacaagg gcggctggat cagcccggcg
      601 tccgccacgc gctggttgaa ccagtcgtag agcggccggt gatcctcgaa ctcgagcgtg
      661 caaagcgagt gggagatgcc ctcgatgcag tccgacagcc cgtgcgccca atcgtaagtc
      721 ggatagatgc accacttgtc cgcggtccgg tgatgcgtcg cgtgtcggat gcggtacatg
      781 gcggggtcgc gcatgttgat gttgggcgag cgcatgtcga tcttggcgcg cagcacgtgc
      841 tcgccgtccc tgtacttgcc gtcgcgcatt tcgcggaaga gcttcaggtt ctcctccacg
      901 cttcggttgc gccacggcga ttccctgccc ggctcggtga aggtgccgcg ggcggcggcc
      961 atctcctcgg cgctctggct gtcgacgtag gcgacccctc gctcgatcaa cgcctcggcg
     1021 aagcggtaga gctggtcgaa gtagtccgag gcatagagct ctttctttcc ccagtcgaag
     1081 ccgagccact tcacgccttc cttgatcgag tcgacgtact cctgctcctc cttctcgggg
     1141 ttggtgtcgt cgaagcgcag gttgcagtta ccgcggtact cggccgcgat gccgaaattc
     1201 aggcagatcg acttgacgtg cccgaagtgc aggtacccgt tcggctcggg aggaaagcgt
     1261 gtgcgcacgc tcgcataccg gcgcgcggcc aggtccgcct cgatgacctg gcggacgaaa
     1321 ttgctcgtgg aaggggcccg gggcgcccgg gcgggcttgc tcatgggaac gggacgggat
     1381 tatcgcactg acgcagcgcg cgattttgga gtattgtcgc gccaccagaa caatatccgg
     1441 gaggttttca tgaagcgcgc ttcattcgca gttctcgcaa tcgcagccgc gctctacggc
     1501 tgcgccacca ccgaatcgca ggacagcagc acgagcgcgg gcacgccgtc gtcgagcgcc
     1561 agccccgcgg caagcacatc caccggcagc ccgagcggca gccaggtcgc cagccgcagc
     1621 gccggcacga gcggcagcgg caccgccgcg agcggcacgc agcgcccgga cattaagcgc
     1681 agcgtgtact acgagttcga caagtacgac gtgaagccgg agtaccgcgc gctcgtggaa
     1741 agccatgcgc gctggctcaa ggccaacccg caggcgaaga tcgtgatcga gggtaacgcc
     1801 gacgagcgcg gcagccgcga gtacaacgtg gcgctcggcc agcggcgcgc cgagtcggtc
     1861 accaagatgc tgacgctcct gggcgcgaag cccgaccaga tcgaggcgat cagctggggc
     1921 gaggagaagc cccgcgcggg cggccacgac gacgcctcct gggcagagaa ccgccgcgcc
     1981 gacttcgctt cgcggtaagc gggttggggt cagaccccaa ccggaaaatc ggggtcagac
     2041 ccaaaccgga agttcgggtc tgaccccggt gttttttcgg ccccgattct tttcctagtc
     2101 cgcgagcccg agcagccgcg ccgggttctt gcgcatcatg aggtcgatgt cggcgtcgga
     2161 aataccttcg cgcttcatcg cagcgatggc agcttccagc ccgtcggggt gcgtcatgtt
     2221 gccgctctgc ccgaggtcgg aggagacgac gaggcttttc gcaccgatct ccttcaccgc
     2281 ctgcgccacg ctcttggcgt tgatctgcgt ccagtgcgtg aggaacggga gcggggcgtt
     2341 gggcccggca aggaactgaa ggaagcagac ttcgatcacc gcgcccatct ccgctacctg
     2401 cttcgcctgc gccattgtca gcccgggcac gttggtaaag ccgtgcgtaa tgagcatgct
     2461 tttgacgcca aggtcccgcc cgcgccgcac tacggcgatg atttcctccg ggtggacatg
     2521 gccggttgcg aggaccagat tctcgcgcgc aatgatcttg aggatcgctt ccatctccgg
     2581 cgtcacctgg ccgccgggcg cgaccaccag gccgcgcgca tcgggcttgg caagggtctt
     2641 cacgtgcttg tcggattcga aagtggggag ccagaccacc ttgccgcgcc cgccgtaggt
     2701 ccggtgcatc cactccaccg cgtcgggatt gacgccgccc acggccttgt tgagcacgat
     2761 gccaccccag acttcgatcc cgggcacgac cttcatcacg agagccgcgc ggcttgcagt
     2821 ctccgcgacg tggttcttga ggaggatgcc gcgcatgccc ttcgatttgg caagctgggc
     2881 gacgtcgatg tcgtccatgt tccggccgaa gacgtccgga tgcgagtgca catgcatgtc
     2941 gatcaccccg gcagcagggc tcaccttcgg cggcggcggc ggaaatccgg cctgtgcgaa
     3001 cgcctgggtg gcggcgaacg cggacgcgat gacgaatgct gctctcatgg ggctcctcct
     3061 ggttgcgcgg catcatagcg caaccgctca agccctgaag tcccactccc cgccctcgaa
     3121 ggtcgcggtc gcgtccatca cgatgcccat gcgctccatg gcgacgagcg aggcgtcctt
     3181 tgccgcaggc gctggcgcgg tgaatgtgcg cggtagaacc tgcaatccct gcggctagtt
     3241 ccgaaggtcc acgcgtagag cggcgcctga catatattgt tcgcgaatga tccgggttct
     3301 gctgctcttc gccgcgcggc gggcgcgggg gcgcatggcg ctgtccgcgg ccggtatcgc
     3361 gctcggcgtg gcgctcggct acggcgtgca tctcgtgaac cgcgccgcgg taagcgatgt
     3421 gacggcggcg gtgcgcgcga tcgcggggga agccgacctc gaggtgcggg gcggccgtag
     3481 cggttttccg gaggcgctgt accccaacat cgcgcgcctt ccgggcgttt cggtggcgag
     3541 cccggccctc gagctcgacg cgggcctccc gggaaccgag cgcattctgc gccttatcgg
     3601 gatcgacatc ttgcgggccg cgatgctcca gccgcagctc gtgctcgagg atcgctatga
     3661 gctgctcgcg cccgaccgga tcgttctctc cccggtggcg gcgcaagcgc tcggcgtggc
     3721 gaagggcgac acggtgtcgc tcgtcggcgc aggcggcacg gtgcaactgc aggttgcggc
     3781 agtggtcgcg tcgttgcgcg gggtttctgc attgaccgac atctccaccg cgcaatggcg
     3841 cttcggccgg ctcggcatcc tcaatcgcat cgacatccgg ctctccaccg gcaccgatcg
     3901 cgagcaggcc atggcggcga tccgggacct tttgccgccc ggcgcgcacg tcgtggccgt
     3961 ggagtcgctg gaagaagcga gcgcttatcc ctctcgttcg taccgggtga acctcaacgt
     4021 gctcgccatg gtggcgctct tcaccggcgg ctttctcgtc ttttcagcgc aggcgctcga
     4081 ggccgcgcgg cgccgcagcg aacatgccct gctcagggtg ctgggcacga agagagcggg
     4141 catttcgaga ctcgtcctga tggaagcggc gatgctcggc gcgattggcg ccgcggtagg
     4201 gcttgcgctg ggctacgcgt tcgcgcatgt cgccgtgcgg gcggcgggcg cagacctcgg
     4261 cgcggggatg ttccaggggg cttcggcgca agtgaagctc tcgggcgccg gcgccgcggt
     4321 gtacttcgta gccggcatcc tggtggcgct gctcggggcg ctggtcccgg cgcgcgacgc
     4381 cgcccgcacg cctgcggcac gcgcgctgaa ggccggggac gagcaggcgc tcttcgagcg
     4441 cgtcgcgccg gtgtggccgg gattgctgct gatcgccatt ggagccgccc tgacgcaggc
     4501 gggaccggtg ggcgggctgc ccgtcttcgg ctacgccgcg atcgcctgcc tgctcgtggg
     4561 cgcgatcgcg ctggtcccga ggatcgccga gctcgtcttc cggtacctgc cgctgccgtc
     4621 ctatccgccg ctgcgcctcg cgctcgggca gcttcgcgcg gcgcccggcc aggcggcggt
     4681 gagcctcgcc gcgatcgtcg cgagcttctc gctcatggca gccatggcga tcatggtggc
     4741 atcgtttcga gagtcggtgg acgagtggct ggcccgcgtg ctgccggcgg aagtctattt
     4801 ccgcaccacg cattcgggcg acacggctct tctcgacgcg gcattcgaag caggcgtgcg
     4861 tgcgctgccc caggtcgcgc gcgccgactt tttgcgcagc ggccgcatcg tgctcgatcc
     4921 ggctcgtccg gcgctcgtgc tgatcgcgcg cgaccgcgcc gaggccactt ttccgcttct
     4981 cgagcgctac gaacgccggg cgggagaccc gccttcggtc tggatcagcg aggccgtggt
     5041 ggatctctac ggatttgcgc ccggggcgcg cctcgagctg ccaatcgcga acaagagcca
     5101 cccggtgctg gtggccgggg tgttccgcga ctatgcgcgc cagcacggca ccgtgcttat
     5161 cgaccgcgcc gactacatag cgcttacggg agaccggacg gcgaacgacg gtgcgctgtg
     5221 gctcgccccc ggcgcgacgc ccgccgaggc gatcgccgcg ctgcgcgcat tgccgggcgc
     5281 agagcagctc gacatcgccg agccgggcga gatccgcgaa gtgtcgctgc gcatcttcga
     5341 tcgcagcttc gccgtcacct atgcgatgga ggccgtagcg gtggtggtgg gcctcttcgg
     5401 gctctcgtcg agcctcggcg ctatcgtgct cgcgcgccgg cgcgagttcg gcgtgctgcg
     5461 ccacctcggc ctcacgcgcg ggcagattcg gggcatgctc gccacggaag gcgctttgct
     5521 tgcgctcgtc ggctcggtag caggccttgc cgccggcgcg gcaatcagcc tggtgcttgt
     5581 ctacgtggtc aaccgccagt cgttccactg gagcatggag ctgcacccgc cgtacctgct
     5641 tctcgccggt ttcatcgtgc tgctggtcgc gctcgcggtg ttgacggccc tcgtagcggg
     5701 cagggaagcg atgggcatgg gcccggtgcg cgcggtgcgc gaggactggt agccatgcgc
     5761 cgcaggacat ttctcgcgct ttcgctgctg ccgttccctg cgctcggcgc caaagtggac
     5821 tatccggcgg tacaaccgaa tacgcgcctg cagttcccgc gcgaccacgg cgcgcatccg
     5881 gaattccgta tcgaatggtg gtacgtgacc ggttggctcg acggcccgct cggctttcag
     5941 atcaccttct tccgtgccag gccggaggaa gcgagcgaca acccgagcgc gttcaatccg
     6001 cggcagatcc tcttcgcgca cgcggcgctc agcgatccga agcgcggccg cctggtccac
     6061 gaccagcgcg cggcacgcgc cggcttttcg ctcgcgcacg ccgagctcga gcgcaccggc
     6121 gtatggatcg acgactggcg gctcgagctt cacggcaacc gctacgaagc acacattgct
     6181 gcccgcgatt tcgcgttcga tttccacctc ctcgcgcatc agctcgtgct gcagggggag
     6241 cagggcttca gccgcaaggg ccaccggccg aacgaggcga gctattacta cagccgcccg
     6301 cagctcaacc tggtcggcaa gctgaacggc cgggacatcg agggaacggc gtggctcgac
     6361 cacgaatggt cgagcgccta catggcgccc gaagcggccg gctgggattg gtgcggcatc
     6421 aatttctccg acggcggctc gctcatggct ttccggatgc gcgacaggaa cggcggcgtt
     6481 cacttcactt ctcccgggtc gacgttccgc gccctgagaa cgtggacctc gccgcgcacc
     6541 ggcgtccagt atccggtgga gatggcggtc aacgacctcg tcctcaagcc gctcatggac
     6601 gaccaggagc tcgactcgcg cgcgagcacc gggacgatct attgggaggg tgctgttact
     6661 gctcatagaa ataccaaccc ggttggccgc ggttatctcg agctcaccgg ctactggagg
     6721 ccgatgaagc tctgaaggac gaaagtgcca cgccggcgag aatcagcgcg atgccggcga
     6781 aatgaaacca gctcgggtgc tcgccgagaa agaccgccgc cagcgctacg cccatcgcgg
     6841 gcatgagatg catcgttacc ccggcgcgcg cggcgcccag ccggcgcacg acatagctcc
     6901 agccggcgta ggcgagaaga agcgagccga tcgccgaata aaggacggcg agcgcgccgg
     6961 gtagcgtgag ctcgacgcgg ggattgtgga caagttccca gccgacccag ggaagcatga
     7021 aaaccacgcc aaagacgcac accatgacga ggagctcgat cgtgttgagc gggtcgcggc
     7081 gcacgcgcag cagcaccgtg tatcccgccc acatcatcat cgaggcgagc gccatcatgt
     7141 cgccgacgtt gaaggacggc accgccagcc gaccctgggt ggcgatgagg tagacgccgc
     7201 agaaggagat cgcgacgccc gtccattgcc tcgggccgat gcgctcgccg agcggcgccg
     7261 ccatgacgag
//
LOCUS       DASUNT010000622         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1219136, whole genome shotgun sequence.
ACCESSION   DASUNT010000622 DASUNT010000000
VERSION     DASUNT010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219136"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VFX94_12555"
     CDS             complement(<1..411)
                     /locus_tag="VFX94_12555"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5769056.1"
                     /translation="MKSSPVCAVLAAGLVACSAPTGPKPAEVPRLENAKRVRTVWSVN
                     VGGAHGFAFQPALADDSVYAAARDGTVTRLDQKTGDRRWRVGLDLRLSGAVGADESVV
                     AVASEEGVVVALEARDGKERWRARVSSEVLAPPAI"
     gene            complement(414..1046)
                     /locus_tag="VFX94_12560"
     CDS             complement(414..1046)
                     /locus_tag="VFX94_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5769057.1"
                     /translation="MALDLEEQEQVAELKAWWKQHGNLIVTVLLVAALAFAGWQGWRW
                     YSASQAAQAGAVFDALAKATQAGDAKAVRDSAGTLLESYPRTLYASMGALMAARFNFD
                     RGDLKAAKAQLEWVVERAPSEDFRDIARLRLAAILLDEKAPDEALKFLEARHGAAYES
                     QYAALRGDALVAKNQPADARAAYQLAIDKAGKEQSAFRDTVRMRLEALGG"
     gene            complement(1046..2380)
                     /gene="hisS"
                     /locus_tag="VFX94_12565"
     CDS             complement(1046..2380)
                     /gene="hisS"
                     /locus_tag="VFX94_12565"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX5769058.1"
                     /translation="MVQKVKPTLLRGFDQEYLPQDQLHFNRLIDIIRRNFELYGFLPI
                     ETPSAERREVLTSKGGVEKEIYALTRLAEADDDEAATKGALRFDLTVPLARYVAMRER
                     ELAFPFRRYQIQRVWRGETPQARKGRFREFYQCDIDIINRDRLSYLAEAEIPSVIYSV
                     FKEMAIGEFRIRINNRKALKGVLQEFRVPEERAAAVLRALDKVEKEEAAKVRAELERE
                     GMSSAAAGELYELIARKRDALQALSTVRAAAPGVAELKQVADAIRQFGVPDSAFAIDL
                     GVVRGLDYYTGTIYETTLTASPELGSICSGGRYDDLASYFTETRLPGVGISIGLTRLF
                     SKLKEAGLLQPLERTPAEVLVTTMDPRYLEQYLTIAGKLRAAGINTEVYLEPAKLGNQ
                     LAYADRKGFRIAIIAGENEFAKNAVQIKNLSTKTPRDVPMTDVVAAARQDLS"
BASE COUNT          347 a          877 c          835 g          432 t
ORIGIN      
        1 gatcgccggc ggcgccagca cctcgctcga gacgcgggcg cgccagcgtt ccttgccgtc
       61 gcgcgcctcg agcgcgacga cgacgccttc ctcgcttgct acggcgacca cgctctcgtc
      121 ggcgccgacg gcgcccgaga gccgcaagtc gaggccgacg cgccagcgcc tgtcgccggt
      181 cttctggtcg agccgcgtga ccgtgccgtc ccgcgccgcc gcgtataccg agtcgtcggc
      241 gagcgcgggc tggaacgcga agccgtgggc tccgcccacg ttcaccgacc agacggtgcg
      301 cacgcgcttc gcgttctcca gccgcggcac ctcggcgggc ttcgggccgg tcggtgccga
      361 gcaggcgacg agcccggccg cgagaacggc gcagaccggg ctcgacttca ccctcaccct
      421 ccgagcgcct cgagccgcat gcgcacggtg tcgcggaacg cgctctgctc cttgcccgcc
      481 ttgtcgatcg cgagctggta ggcggcgcgc gcatccgccg gctggttctt ggccacgagg
      541 gcgtcgccgc gcaacgcggc gtactgcgac tcgtaggccg cgccatgcct cgcctcgagg
      601 aacttgagcg cctcgtccgg cgctttctcg tcgagcagga tcgccgcgag ccgcaggcgc
      661 gcgatgtcgc ggaagtcttc cgacggcgca cgctcgacca cccactcgag ctgcgccttt
      721 gcggccttga ggtcgccgcg gtcgaagttg aagcgcgccg ccatgagggc gcccatcgat
      781 gcgtagaggg tgcgcggata gctctcgagc agcgtgccgg cggagtcgcg caccgccttc
      841 gcgtcgcccg cctgcgtcgc cttggcgagg gcgtcgaaca cggcgcccgc ctgcgccgcc
      901 tggcttgcgc tgtaccaacg ccagccctgc cacccggcga acgcgagcgc cgcgacgagc
      961 agcacggtaa cgatgaggtt gccgtgctgc ttccaccacg ccttcagctc ggcgacctgt
     1021 tcctgctctt caagatcgag ggccatcagc tgagatcctg cctggccgcg gctacgacat
     1081 cggtcatggg aacgtcccgg ggagtcttgg tcgaaaggtt cttgatctgg actgcatttt
     1141 tggcgaactc gttctcgccc gcgattatgg cgatgcggaa gcccttgcgg tcggcgtagg
     1201 caagctggtt gcccagcttc gcaggctcga ggtacacctc ggtattgatc ccggcggccc
     1261 ggagcttccc ggctatggtc aggtattgct ccaggtagcg cggatccatg gtggtgacca
     1321 gcacctccgc cggcgtgcgc tcgagcggct ggagcagccc ggcttccttc aacttcgaga
     1381 agagccgcgt cagcccgatc gagattccca cgccgggaag ccgcgtttcg gtgaagtagc
     1441 tcgcgaggtc gtcgtagcgc ccgccggagc agatgctgcc gagctctgga gacgccgtga
     1501 gcgtggtctc gtagatcgtg ccggtgtagt agtccaggcc gcgtaccacg cccaggtcga
     1561 tcgcaaaagc ggagtccggc acgccaaact ggcggatcgc gtcggcgacc tgcttcagtt
     1621 ccgccacgcc cggggcggcc gcccggaccg tggacagcgc ctgcagggca tcgcgcttgc
     1681 gcgcgatgag ctcgtagagc tcgccggcgg cggcgctcga catcccttcg cgctccagct
     1741 ccgcgcggac cttcgccgcc tcctccttct cgaccttgtc gagggcgcgc agcaccgcgg
     1801 cggcgcgctc ctccggcacg cggaactcct gcagcacgcc tttcagggcc ttgcggttgt
     1861 tgatgcggat gcgaaactcg ccaatcgcca tttccttgaa cacgctgtag atcacgctcg
     1921 ggatctcggc ctcggccagg tacgaaagcc ggtcccggtt gatgatgtcg atatcgcact
     1981 ggtagaactc gcgaaagcgg cccttgcgcg cctgcggcgt ctcgccgcgc cacacgcgct
     2041 ggatctggta gcggcggaac gggaacgcca gctcgcgctc gcgcatcgcg acgtagcgcg
     2101 cgagcggcac cgtcaggtcg aagcgcagcg cgcccttggt ggcggcctcg tcgtcgtccg
     2161 cctcggccag ccgcgtgagc gcgtagatct ccttctccac gccgcccttc gaggtgagca
     2221 cctcgcggcg ctcggccgac ggcgtctcga tcggcaggaa gccgtagagc tcgaagttgc
     2281 gccggatgat gtcgatcagc cggttgaagt gcagctggtc ctggggcagg tattcctggt
     2341 cgaagccccg cagcagggtt ggcttgactt tctgcaccat caaagcgctt tgatcgggat
     2401 tttcttcccg tacttctgct ggacgtagtc ctcgaccagg gcctggaatt cctcggcgat
     2461 gcgcgcgccc ttgagcgtta cggtcttctt g
//
LOCUS       DASUNT010000623         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_518688, whole genome shotgun sequence.
ACCESSION   DASUNT010000623 DASUNT010000000
VERSION     DASUNT010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518688"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..2089
                     /locus_tag="VFX94_12570"
     CDS             38..2089
                     /locus_tag="VFX94_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5769059.1"
                     /translation="MTESASPPSFSELGLSDAVVAALNDVGYENPTPIQAQTIPVILE
                     GADLLGQAQTGTGKTAAFALPILSKIDVELRATQALILAPTRELAIQVAEAFQKYAAK
                     MPGFHVLPIYGGQSYTPQLKGLKRGAHVVVGTPGRLLDHVKSGALSLESLKFLVLDEG
                     DEMLQMGFVDDIEFLFQQAPEEKQVALFTATLPHAIRRIAQTHMREPREISVQSRGEA
                     APKIRQRYWLVSGLHKLDALTRLLEAEKFDAILVFVRTKLETVDLAQRLEARGFSASA
                     LNGDMEQRQREQTIAQLKNGKIDILIATDVAARGLDVERISHVINYDIPYDSETYIHR
                     IGRTGRAGRQGDAILFVSPRERNMLRIIERVTRQQIEEMKMPSVADVNELRVVKFKEK
                     ITAAVLSGAGKEFQSLLEQVETEGNIPAIEIAAALASMVQGSAPLFLKQKAESEEPKA
                     PPRIDGSQPTQTYRIEVGHAHGVLPGNIVGAIANEAGIEGRNIGHIDIREDHSYVDLP
                     TLPDEMMASLQSVRVRGEAIRIQRVDSKPDKPKFSGKSRRPDRMAEAASFEDTRGERR
                     PYAARKPYIGGGNRFDKAGEPRPHESRRAQSRNYKGTPRFAGAGEPRVESFTPVGDAD
                     AAQVARPRPAGKPRFGGKPSFGGKRSFEKPGGFKKKFTGKAAGAKGSYKGGKAFRNKA
                     G"
BASE COUNT          426 a          774 c          696 g          324 t
ORIGIN      
        1 gctttcccca ctttcctcac ccggcaccaa cacccttatg actgaatccg cctccccgcc
       61 cagcttttcc gagttgggat tgagcgacgc cgtcgtcgcc gccctcaacg acgtgggcta
      121 cgaaaacccc acccccatcc aggcccagac catcccggtc atcctggaag gcgcggacct
      181 gctcggccag gcgcagaccg gcaccggcaa gacggcggcc ttcgccctgc ccatcctctc
      241 gaaaatcgac gtggagctgc gcgccacgca ggcgctgatc ctcgcgccca cgcgcgagct
      301 ggccatccag gtcgccgagg ccttccagaa atacgccgcc aagatgccgg gcttccacgt
      361 gttgcccatc tacggcggtc agagctacac gccccagttg aaaggcctca agcgcggcgc
      421 gcacgtggtg gtcggcaccc ccggcagatt gctggatcac gtgaagagcg gcgccctgag
      481 cctcgagtcg ctgaagttcc tggtgctcga cgaaggcgac gagatgttgc agatgggctt
      541 cgtcgacgac atcgagttcc tgttccagca ggcgccggaa gaaaagcagg tggccttgtt
      601 caccgccacg ctgccgcacg ccatccgccg catcgcccag acccacatgc gcgaaccgcg
      661 cgagatcagc gtgcagtcgc gtggcgaggc cgcgccgaag atccgccagc gctactggct
      721 ggtgagcggc ctgcacaagc tcgatgcgct cactcgcctg ctcgaggccg agaagttcga
      781 tgccatcctg gtgttcgtgc gcaccaagct cgagaccgtc gacctggcgc agcgcctcga
      841 ggcgcgtggc ttctccgcct cggcgttgaa cggcgacatg gagcagcgcc agcgcgagca
      901 gaccatcgcg caactcaaga acggcaagat cgacatcctg atcgcgaccg acgtggccgc
      961 gcgcgggctc gacgtcgagc gcatctcgca cgtcatcaac tacgacatcc cgtacgactc
     1021 cgagacctac atccaccgca tcggccgcac cgggcgcgcg ggtcgccagg gcgacgccat
     1081 cctgttcgtc agcccgcgcg agcggaacat gctgcgcatc atcgagcgcg tcacgcgcca
     1141 gcagatcgaa gagatgaaga tgccgagcgt ggccgacgtc aacgagctgc gcgtggtgaa
     1201 gttcaaggag aagatcacgg cggcggtgtt gtccggcgcc ggcaaggaat tccagtcgct
     1261 gctcgagcag gtggaaaccg aaggcaatat tcccgccatc gaaatcgccg ccgcgctcgc
     1321 cagcatggtg cagggttcgg cgccgttgtt cctcaagcag aaggccgagt ccgaggagcc
     1381 caaggcgccg ccgcgcatcg acggcagcca gccgacgcag acctatcgca tcgaagtcgg
     1441 tcacgcgcac ggcgtgcttc ccggcaacat cgtcggcgcg atcgccaatg aagccggcat
     1501 cgaaggtcgc aacatcggcc acatcgacat tcgcgaggat cacagctacg tggatctgcc
     1561 cacgctgccc gacgagatga tggcgagcct gcagagcgtg cgcgtgcgtg gcgaagccat
     1621 ccgcatccag cgcgtggatt cgaaacccga caaaccgaaa ttcagcggaa aatcccgccg
     1681 tcccgaccgc atggccgagg ccgcttcgtt cgaggacacg cgcggcgagc gtcgccccta
     1741 tgccgccagg aagccgtaca tcggtggtgg caatcgcttc gacaaggccg gcgagccgcg
     1801 accccatgaa agccgccgtg cccaatcgcg caactacaag ggcacgccgc gctttgccgg
     1861 cgccggtgag ccgcgcgtcg agagcttcac gccggtcggc gacgcggacg ccgcgcaggt
     1921 cgcgcgtccg cgacctgcgg gcaagccgcg ctttggcggg aaaccttcgt tcggcggcaa
     1981 gcgctcattc gaaaagccag gcggcttcaa gaaaaaattc accggcaagg cggctggagc
     2041 caagggcagc tacaagggcg gcaaggcctt tcgcaacaag gcgggctgag caccggcact
     2101 agtaaggcgg gcgcagggcc tcgggctcgg tgccattgcc ttcatcggtc tccggcaggc
     2161 gcagctcgtc gaaatactcg cgcatgttct cgaacatgtc gccgcgcacc ttcgccttgc
//
LOCUS       DASUNT010000624         4803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_255487, whole genome shotgun sequence.
ACCESSION   DASUNT010000624 DASUNT010000000
VERSION     DASUNT010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255487"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VFX94_12575"
     CDS             complement(<1..726)
                     /locus_tag="VFX94_12575"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5769060.1"
                     /translation="MKRAVLFSALTVLAGFAAAQSYPSRPVTIVVPFAAGGPTDIIAR
                     ITGEYFSKALGQQFVVENIAGAGGTTGITRGMQAKPDGYTIMMGHMGTHGAAPALYKN
                     LKYDPAKDFTPLGLVAGTPILIVARKDFPAANLKEFVAKVKDPAAKVKQAHAGVGSVS
                     FTTCTLLTSQLGAKSIVAAYRGTGPALNDLVGGQVDYMCDQIVNLVEQVKGGSIRAYA
                     IATAERSPALPDVPTTKEAGLPEY"
     gene            complement(743..2242)
                     /locus_tag="VFX94_12580"
     CDS             complement(743..2242)
                     /locus_tag="VFX94_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5769061.1"
                     /translation="MELLSNLALGFATALSPTNLFLGFIGCLVGTLIGVLPGVGPIAT
                     IAMLMPLTLKVDPTGALIMLAGIYYGAQYGGSTTAILVNMPGEVTAVVTALDGHEMAK
                     QGRAGAALGMAAIGSFFAGSVATLVIAAIGAPLTRLALLFGPAEYFALMVFGLGLAIV
                     LARGSVLKAVIMVVLGLLLSTVGTDLETGQDRLTLGIAELSDGVDFAVLAMGLFGIAE
                     ILRNLENPESRNVAIAQIGRLLPTRAELKRCFGPIVRATGVGSILGILPGNGAVLGPF
                     ASYAMEKKLAKDPSRFGKGAIEGVAGPEAANNAGAQTSFIPMLTLGIPGNAVMALMIG
                     ALMIQGIAPGPQVMNEKPELFWGLIASMWVGNAFLVILNLPLIGLWVRLLKVPYRLMF
                     TTILLFCAIGIYSINNNPYDVYFTAFFGLVGYLFIKLGLEPAPLLLGFVLGRLMEEKM
                     RQALALSEGSFMTFVERPVAAVLLALAVGIILIAVLPAVSRSRQKAFQE"
     gene            complement(2244..2696)
                     /locus_tag="VFX94_12585"
     CDS             complement(2244..2696)
                     /locus_tag="VFX94_12585"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX5769062.1"
                     /translation="MRGKLLRQDLLGGLVIIAVAAFAYWQARELSTAAAGGMGPGTLP
                     KGLAVLLAGLGVLLVLHSLKSERMGRWSVRGPLFVLGGLVLFGVTVRPLGLAVAGPLA
                     IAAASFASSEVRWRETLISGVLMTVFCIALFKYALGLPIPLAPWLVGY"
     gene            2779..3228
                     /locus_tag="VFX94_12590"
     CDS             2779..3228
                     /locus_tag="VFX94_12590"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5769063.1"
                     /translation="MYKHILLPTDGSRRAGKGAAEGVRLAKALGAKVTGVYVAPPFYR
                     PPTYLEGGVYIPGVAPKVYRKHSEALAKKAFAPIEAAARKARVRCATRLLTANRASDG
                     ILKAARALGCDAIAMSSRCRRALDAVLLGSETRRVLLSSKVPVVVFR"
     gene            complement(3225..4376)
                     /gene="prpC"
                     /locus_tag="VFX94_12595"
     CDS             complement(3225..4376)
                     /gene="prpC"
                     /locus_tag="VFX94_12595"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEX5769064.1"
                     /translation="MASVSEILPKAKKSVALSGVPAGNTALCSVGRTGNDLHYRGYDI
                     LDFADQAEFEEIAYLLVHGRLPTREELGQYKKHLRTLRALPQMLKAALECIPEDAHPM
                     DVLRTGVSVLGTLEPEKPDHGQPGAQRIADRLMASLGSMLLYWYHYAGEGRRIDVETD
                     DESIGGHFLHLLHQKKPKDEWVRAMHTSLILYAEHEFNASTFTARVIAGTNSDMYSAI
                     GGAIGALKGPKHGGANEAAFEVLTRYADPDVAERDILRRLEAKELVIGFGHPVYTIAD
                     PRNEVIKAVASRLARDRGQNLFDIAERLEQVMWREKRMFANLDWYSAVSYHAMGVPTD
                     MFTPLFVISRTSGWAAHVIEQRADGKIIRPSANYTGPENRRWVPLEGRA"
     gene            complement(4364..>4803)
                     /locus_tag="VFX94_12600"
     CDS             complement(4364..>4803)
                     /locus_tag="VFX94_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5769065.1"
                     /translation="DLDRVYSYFPRLKDRRSSLAGYTSGGEQQMTAVGRALMARPKMI
                     LLDEPSMGLAPQIVHEIFEIVQNLNKREKVSFLLAEQNAVMALRFAEFGYILENGRVV
                     IEGTSAQLAENEDVKEFYLGISSGKQKSFRDVKHYRRRKRWLA"
BASE COUNT          832 a         1580 c         1588 g          803 t
ORIGIN      
        1 gtactcgggc aggcctgcct ccttggtggt cgggacgtcg ggcagcgcgg gcgaacgctc
       61 cgccgtcgcg atggcgtagg ccctgatgct gccgcccttg acctgctcga caaggttgac
      121 gatctggtcg cacatatagt ccacctgccc gccgacgaga tcgttgagcg ccggtccggt
      181 gccgcggtag gccgccacga tgctcttcgc gccgagctgc gacgtgagga gcgtgcaggt
      241 ggtgaacgac accgagccca cgccggcgtg cgcctgtttg accttggcgg cgggatcctt
      301 caccttggcg acgaattcct tgagatttgc ggcgggaaag tccttgcgcg ccacgatcag
      361 gatcggcgtg ccggcgacga ggccgagcgg cgtgaagtcc ttcgccgggt cgtacttgag
      421 gttcttgtac agcgcgggcg cggcgccgtg cgtgcccatg tggcccatca tgatcgtgta
      481 gccgtcgggc ttcgcctgca tgccgcgcgt aatgccggtg gtgccgccgg cgccggcgat
      541 gttctcgacc acgaactgct ggccgagcgc cttcgagaag tattcgccgg tgatgcgggc
      601 gatgatgtcg gtcggcccgc ccgcggcgaa cggcaccacg atggtcaccg ggcgcgacgg
      661 atatgattgt gccgccgcga agcctgcaag cacagtcaat gcggaaaaca gaacagcgcg
      721 tttcatgatt tctcctctga tttcattcct ggaatgcttt ctggcggctg cggctcactg
      781 caggcagcac cgcgatcagg atgattccga cagcgagcgc gagcagcacg gcggcgaccg
      841 ggcgctcgac gaaggtcatg aagctgccct cggaaagcgc gagcgcctgg cgcatctttt
      901 cctccatcag cctgccgagc acgaagccga gcagcaacgg cgccggctcc aggccgagct
      961 tgatgaagag atagccgacc agcccgaaaa acgccgtgaa gtagacgtcg taggggttgt
     1021 tgttgatcga atagatgccg atcgcgcaga acagcaggat cgtggtgaac atcagccggt
     1081 acgggacctt gagaagtcgc acccacagcc cgatcagcgg caggttgagg atcacgagga
     1141 acgcgttgcc cacccacatc gaggcgatca gcccccagaa gagctccggc ttctcgttca
     1201 tcacctgcgg gccgggcgcg ataccctgga tcatcagcgc gccgatcatc agcgccatta
     1261 ccgcgttgcc cggaatgccg agcgtcagca tggggatgaa cgaggtctgc gcgccggcgt
     1321 tgttcgcggc ttccggcccc gccacccctt cgatcgctcc cttgccgaag cgcgaggggt
     1381 ctttcgcgag cttcttctcc atggcatagg aagcgaacgg gccgagcacc gcgccgttgc
     1441 ccggcaggat gccgagaatc gagccgacgc cggtggcgcg cacgatcggg ccgaagcagc
     1501 gcttgagctc cgcgcgcgtc ggcagcaggc gcccgatctg cgcgatcgcc acgttgcgcg
     1561 actcgggatt ctcgaggttc ctcaggatct cggcgatgcc gaagaggccc atggcgagca
     1621 cggcgaagtc cacgccgtcg gagagctcgg cgatgccgag cgtgaggcga tcctggccgg
     1681 tttcgaggtc tgtccccacg gtggagagca gcagcccgag caccaccatg atcaccgcct
     1741 tgagcaccga gccgcgcgcg agcacgatgg cgagccccag gccgaacacc atcagggcga
     1801 aatactcggc ggggccgaag agcagcgcaa gccgcgtgag cggcgcgccg atggcggcga
     1861 tgacaagcgt cgccacgctg ccggcgaaga acgagccgat cgccgccatg ccgagcgcgg
     1921 cgcctgcgcg accctgcttc gccatctcgt ggccatcgag cgcggttacc accgcggtca
     1981 cctcgcccgg catgttgacg aggatcgcgg tggtcgagcc gccgtactgc gcgccgtagt
     2041 agatgccggc gagcatgatg agagcgccgg tcggatcgac cttcagggtg agcggcatca
     2101 gcatcgcgat cgtggcgatc gggccgacgc cgggcagcac cccgatcagc gtgccgacga
     2161 ggcagccgat gaagccgaga aaaagattgg tgggggaaag tgcggtcgcg aagcccagag
     2221 caagattgct caggagctcc atgtcaataa cctaccagcc agggcgccaa cggaatcggc
     2281 agcccgagcg cgtatttgaa gagcgcgatg cagaaaaccg tcatcagcac cccggagatc
     2341 agcgtctcgc gccagcgcac ctcgctcgag gcaaacgacg ccgcggcgat cgcgagcggc
     2401 ccggcgaccg cgagcccgag cggccgcacc gtcacgccga agagcacgag cccgccgagg
     2461 acgaagagcg gtccgcgcac cgaccagcgc cccatgcgct cactcttcaa ggaatgcagg
     2521 acgagcagca cgcccagccc tgcgagcagc accgcgagcc ccttgggcag ggtgccgggg
     2581 cccatgccgc ccgccgcagc ggtcgaaagc tcgcgcgcct gccagtacgc aaacgcggcg
     2641 accgcgatga tcacgaggcc gccgagcaaa tcctgtcgca ggagttttcc gcgcatcagc
     2701 gccattcttc taacagactg cttccgggtt ttgatccacg tcaatagggc tcaccgcagc
     2761 cggggcacca tgcctcccat gtacaagcat atcctgcttc cgacggatgg ctcgcggcgc
     2821 gccggcaagg gcgccgccga gggggtgcgg cttgccaagg cgctcggggc gaaggtcacc
     2881 ggcgtctacg ttgccccgcc cttttaccgg ccgccgacct atctcgaagg cggcgtctat
     2941 atccccgggg tcgcgccgaa ggtgtatcgc aagcacagcg aagcgctggc gaaaaaagcg
     3001 tttgcgccga tcgaagcggc cgccaggaag gcgcgcgtgc gctgcgcgac ccggttgctc
     3061 accgcaaacc gggcgagcga tgggatcctc aaggcggcgc gggcgctggg atgcgacgcg
     3121 atcgcgatgt cctcgcgctg ccgccgcgcc ctcgacgcag tgctccttgg cagcgagacg
     3181 cgccgggtgc tcctcagctc gaaggttccg gtcgtcgtct ttcgctaggc gcggccttcg
     3241 agcgggaccc agcgccggtt ctccgggccc gtgtagttgg cgctcgggcg gatgatcttg
     3301 ccgtcggcgc gctgctcgat gacgtgcgcc gcccaccccg aggtgcgcga aatcacgaaa
     3361 agcggcgtga acatgtcggt cggcacgccc atcgcgtggt acgagaccgc gctgtaccag
     3421 tcgaggttgg cgaacatcct cttctcgcgc cacatgacct gttccagccg ctcggcgatg
     3481 tcgaacaggt tttgacctct atctcttgca agacggctcg caaccgcctt gattacctca
     3541 ttgcgggggt ccgcgatggt gtagaccggg tggccgaagc cgatcaccag ctccttcgcc
     3601 tcgaggcgcc gcaggatgtc gcgctcggct acatcaggat cggcatatcg ggtgagcact
     3661 tcgaaagccg cctcgttcgc gccgccgtgc ttcggcccct tgagcgcgcc gatcgcgcca
     3721 ccgatcgccg agtacatgtc cgaattggtg ccggcgatga ctcgcgcagt aaaggtgctg
     3781 gcgttgaatt cgtgctcggc gtagaggatg agcgaggtat gcatcgcgcg cacccactca
     3841 tcctttggtt tcttctgatg gagcagatgc aagaaatggc cgccgatgga ttcgtcgtcg
     3901 gtctcgacgt cgatgcgccg gccctcgccg gcgtagtggt accagtagag cagcatcgag
     3961 ccgagcgagg ccatcagccg atcggcgatg cgctgcgcgc cgggctggcc atggtcgggc
     4021 ttttccggct ccagcgtgcc tagcaccgac acgccggtgc gcaggacatc catgggatgc
     4081 gcgtcttccg ggatgcattc gagcgccgcc ttcagcatct gcggcagcgc cctcagggtc
     4141 ctgaggtgct tcttgtactg tcccagctcc tcccgggtgg gaagcctccc gtgcaccagc
     4201 aggtaggcaa tctcctcgaa ctcggcctga tcggcgaaat cgaggatgtc gtagccacga
     4261 tagtggaggt cattgccggt gcgtccgacg ctgcaaagcg ccgtgtttcc ggcaggcacg
     4321 ccggacagcg cgaccgactt tttagccttg ggaaggattt cgcttacgct agccatcttt
     4381 tcctccgccg gtagtgcttt acgtcgcgga agctcttctg cttgccgctc gaaatgccga
     4441 ggtagaactc cttcacgtcc tcgttctcgg cgagctgcgc cgaggtgccc tcgatcacca
     4501 cgcgcccgtt ctcgaggatg tagccgaact cggcgaatct gagcgccatc accgcgttct
     4561 gctcggcgag cagaaagctc accttctcgc gcttgttgag gttctgcacg atctcgaaga
     4621 tctcgtgcac gatctgcggc gcaagtccca tcgaaggctc gtcgagcaga atcatcttcg
     4681 gccgcgccat cagcgcgcgt ccgaccgcgg tcatctgctg ctccccgccc gaggtgtagc
     4741 cggcaaggct cgaacggcgg tccttgagtc gcgggaagta gctgtacacg cggtcgaggt
     4801 cgc
//
LOCUS       DASUNT010000625         3346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_955423, whole genome shotgun sequence.
ACCESSION   DASUNT010000625 DASUNT010000000
VERSION     DASUNT010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955423"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VFX94_12605"
     CDS             <1..428
                     /locus_tag="VFX94_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769066.1"
                     /translation="VTAGTFVQPFYSAEMAKGGLRKLFTATEAVGYDHELLDIFFSEK
                     FLKANPDAVRAFLADYVAVTKYYLANTAQAKTDLHKAGFVKTPLPIYLKVSDWKRDPN
                     ARVDVESLKKLSTFMREKLQWLEKPVNVDSMVDLSYLPR"
     gene            complement(435..1271)
                     /locus_tag="VFX94_12610"
     CDS             complement(435..1271)
                     /locus_tag="VFX94_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769067.1"
                     /translation="MKARASALHLVAAALFALAPGAQAQPDERAATAQRLMARSGLAV
                     QLRGFTDQIVTDIRQSATRFDENTVERLIQSAKQAYRPEALQQDITARVAKKLTVGDM
                     KAALAWLETDAGARITRAEEVASTSHDMQGLSKFAEGLRGKPLPAQRERLISDLIWAT
                     GAVRTAATAAETMAFGIAYGLDSLQPRERRMGEARVRGNIRDAMPADKMQGLFAQQLP
                     LMYAYAYREIPDADLARYLNFLKGSTGRRYQDAMNAAFMEGLSRASIQMGELAGARQR
                     QT"
     gene            1385..1603
                     /locus_tag="VFX94_12615"
     CDS             1385..1603
                     /locus_tag="VFX94_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769068.1"
                     /translation="MKLSRALRASLSARAARHAAAGREALRTGLHDGDRAAVFELDDD
                     LLAVLLVDLALDRVAEQPADRRTLCTVP"
     gene            complement(2192..>3346)
                     /locus_tag="VFX94_12620"
     CDS             complement(2192..>3346)
                     /locus_tag="VFX94_12620"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5769069.1"
                     /translation="PIATETFATPTAEKKLSVEVAKLAGIKEGRAVLRVVARDGALFR
                     GNETVLQREITIDITPPTLQLVAEDRYINFGGAGAIVYKPSADTATSGVRVGDHFFPG
                     HKGQVKGKEDHYVVFFAHPYNAPPNAKAFLVATDKAGNTREMAISYELKNVKYRKSTI
                     TVSDNFIQSKVAPLLSAGDAKEGSAKETFLAVNNRLRKQNDDRIAAITKKSSSARQWD
                     GAFVQLSNSKVEANFADERTYTYKGVAIDKAYHLGYDLSVTERYPAEAANAGTVVFAG
                     DLGIYGNTVILDHGLGLFTLYSHLSSIDVKEGETVKQRQALGRTGQTGLAAGDHLHYG
                     VYLHGVAVLPVEWWDAKWIKDNIAPKLEGMSGEEIAVMQQPRKAAPKRRR"
BASE COUNT          521 a         1120 c         1077 g          628 t
ORIGIN      
        1 aggttaccgc cggcaccttc gtgcagccgt tctactccgc cgagatggcg aagggcggac
       61 tgaggaagct cttcaccgcc accgaggccg tcggctacga ccacgagctg ctcgacatct
      121 tcttcagcga aaaattcctg aaggccaacc ccgatgcggt gcgcgccttc ctcgccgact
      181 acgtcgcggt cacgaagtac tacctcgcca acaccgcgca ggcgaagacc gacctgcaca
      241 aggccggctt cgtgaagacg ccgctgccga tctacctcaa ggtgtccgac tggaagcgcg
      301 atccgaacgc gcgcgtcgac gtcgagagcc tgaaaaagct ttccaccttc atgcgcgaga
      361 agctgcagtg gctcgagaag ccggtgaacg tcgactcgat ggtcgatctg agctacctgc
      421 cgcgctgagc ggctctaggt ctggcgctgc cgcgcgccgg cgagctcgcc catctgaatg
      481 ctcgcccgcg agagcccttc catgaaggcg gcgttcatcg cgtcctggta acgcctgccg
      541 gtcgagccct tgaggaagtt caggtaccgg gcaagatcgg catccggaat ttcccgatag
      601 gcataggcgt acatgagcgg cagctgttgc gcgaaaagcc cctgcatctt gtccgcgggc
      661 atcgcgtcgc ggatattgcc gcggacgcgc gcctctccca tgcggcgctc gcgcggctgg
      721 agcgagtcca ggccgtaggc gatgccgaac gccatcgtct ccgccgcggt ggccgctgtg
      781 cgcaccgcgc ccgtggccca gatgagatcg gagatgagcc gctcgcgctg agccggcagc
      841 ggcttgccgc gcaggccctc ggcgaacttc gatagcccct gcatgtcgtg cgacgtggac
      901 gccacctcct ccgcacgcgt gatgcgcgcg ccggcatcgg tttcgagcca cgcgagcgcg
      961 gccttcatgt cgcccacggt gagcttcttc gccacgcgcg ccgtgatgtc ctgctgcagc
     1021 gcttccggcc ggtaggcctg cttcgccgat tggatcaggc gctcgacggt gttttcgtcg
     1081 aatctcgtcg cgctttgccg gatgtcggtg acgatctggt cggtaaagcc gcgcagctgc
     1141 accgcgagcc cggagcgcgc catcaggcgc tgcgcggtcg cggccctctc gtcaggctgg
     1201 gcttgagctc ccggcgcaag cgcgaaaagc gccgcggcaa cgagatgcag tgccgatgct
     1261 cttgctttca tgtttcgctc cctcgcgatt ccggccaaag acgccggaaa gcgccactat
     1321 gcgggcaggg ggttccgagg ggtgcaagaa gctgcaagcc gtcgtgacgt agttcactcc
     1381 agttatgaaa ctcagccgcg ctctacgcgc gagcctttcg gcccgcgcag cacgccacgc
     1441 ggccgccggg cgcgaagccc tgcgaaccgg gctgcacgat ggcgatcgtg ctgccgtttt
     1501 cgagctggat gacgatctcc tggccgtcct gctcgtcgat cttgcgctcg atcgcgttgc
     1561 cgagcagccc gccgatcgcc gtacgctctg caccgtgccg taggaaacgc tctgcacggc
     1621 gttcgcctcg ctgtgcgcga agtcctgggc gctcgcgccc gcgcaggccg cgagcatgaa
     1681 gagaatgagt ccctatctca tttgagttgc tccaagtgct gtccgtttga atgggaggga
     1741 ggtgagaaac acattgagcc gctcgcatca cgcgaggttt tcagggcgta cggaccgctc
     1801 gttccatttg ctacatccgc gacgaccgca cggtacgcgt gcgtgacgaa cgcccgccat
     1861 catgcgttca agagaggtct atatgaagaa gactcacatc cgtaacaccc tcggcatcac
     1921 catcctcgcc ctcgcggccg ccggctgcgc cagcgatccg atgtccgctg ccgcgggcgg
     1981 cacaagcgct gcgccgacag tcgacccggc gcccgtgcgg gcccactaca gcgattcggt
     2041 gacgtccaac ttcaacgccc ggattaccgg acggattgga actgcccgaa gtgctacaag
     2101 taggttcggt cgcgccggcg gatgagctgg cgcggctgat gccgcgcagc cgcggggcca
     2161 ggcccgagct caccctcgaa gaggggacct gctagcgccg gcgtttcggc gcggccttgc
     2221 gcggctgctg catgacggcg atctcctcgc cgctcatgcc ctcgagcttc ggcgcgatgt
     2281 tgtccttgat ccacttcgcg tcccaccact ccaccggcag gaccgcgacg ccgtgcaggt
     2341 agacgccgta gtgcaggtgg tcgccggcgg cgaggccggt ctggccggtc ctgccaagcg
     2401 cctggcgctg cttcacggtt tcgccttctt tcacatcgat cgagctcagg tgactgtaga
     2461 gcgtgaagag cccgaggccg tggtccagga tgaccgtgtt gccgtagatt ccgaggtcgc
     2521 ccgcgaagac caccgtgcct gcgttcgccg cctcggccgg atagcgctcg gtgaccgaga
     2581 ggtcgtagcc gaggtggtac gccttgtcga tcgccacgcc cttgtaggtg taggtgcgct
     2641 cgtcggcgaa gttcgcttcc accttggagt tcgagagctg gacaaacgcg ccgtcccact
     2701 gccttgcgga actggacttc ttcgtgatcg ccgcgatcct gtcgtcgttc tgcttcctca
     2761 ggcggttgtt gacggcgagg aacgtctcct tcgcgctgcc ctccttggcg tcgccggcgc
     2821 tcaggagcgg agccaccttg ctctggatga agttgtcgga tacggtgatg gtgctcttgc
     2881 gatacttcac gttcttgagc tcgtaggaaa tggccatctc gcgggtattg cccgccttgt
     2941 ccgtcgcgac gaggaacgcc ttggcgttcg gcggtgcgtt gtaggggtgg gcaaagaaca
     3001 cgacgtagtg atcctccttc cccttcacct ggcccttgtg gcccgggaag aagtgatccc
     3061 caaccctcac cccgctggtc gcggtatcgg cggagggctt gtacacgatc gcgcccgccc
     3121 cgccgaagtt gatataccgg tcttccgcga cgagctgcaa cgtgggcggc gtgatgtcga
     3181 tggtgatctc cctctgcagc accgtctcgt tgcccctgaa gagcgcgccg tcccgggcga
     3241 cgacgcgcag gacggcgcgg ccttccttga tcccggcgag cttcgccacc tcgacgctga
     3301 gcttcttttc cgcggtcggg gtcgcgaatg tctcggtggc gatcgg
//
LOCUS       DASUNT010000626         2155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_519235, whole genome shotgun sequence.
ACCESSION   DASUNT010000626 DASUNT010000000
VERSION     DASUNT010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519235"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFX94_12625"
     CDS             complement(<1..380)
                     /locus_tag="VFX94_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HEX5769070.1"
                     /translation="MKLRIVVALAAAAVLGGCAKEQTRYGDARGVETVTNEYGSTDLQ
                     LMAESMARSMLQAPVIASGNLPVVTVQEVRNKTSEYIDTRAITDSIRAELQKGGRVRF
                     AVDQAGMNQQADELKRQQSGLYAK"
     gene            complement(377..811)
                     /locus_tag="VFX94_12630"
     CDS             complement(377..811)
                     /locus_tag="VFX94_12630"
                     /inference="COORDINATES: protein motif:HMM:NF018889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HEX5769071.1"
                     /translation="MKTTLVIAAIAASALVAGCAAKKGFEKPGYAFGCPIGETKVSSM
                     DDLVKAKVLSEGRRTTIQATEMRCSQAGDLMRIDANLNNDSRSVKRIAYKFRWMDREG
                     MRAWEEEAWKPLMLYENSNLVINTISPTNKAVDFRLILRSEE"
     gene            complement(826..>2155)
                     /locus_tag="VFX94_12635"
     CDS             complement(826..>2155)
                     /locus_tag="VFX94_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769072.1"
                     /translation="AMRSYDAELYVTLERASTGAVDDAIRLLESNNRKSDKDLLYYLE
                     LGMLQRLAGRYEESQKAWKSAAARIEAQNAGGLAEASSFVRGVSSYVVSDKLRAYPGY
                     DYEKVMLLTYLALNHLAMGDFDGARVAIKQAHELEAQIAEARAKQYAEVEESARQRGA
                     RTSFKELNGYPIETIDTPEVNALRNGYQSALSHYLAGFIYEALGEPSLAAPGYRLANE
                     LQPGQPLLEEALRGLDERVAGARDDGMTEVLFIVGSGSAPAIQSRQFLLPIFIETRMV
                     LIANAFPVMVATSWTPLPSELTLDGRSLAAARITSVDLMARRRLMDDMPAIMLRATIR
                     STVNATLQYQAQRASDREQSAAMAIAAGILTASSVLLASADDRTWRALPSEVSIARAR
                     VPRGVHTITLHTPDGPRSASATVSGRYAVVDLRLLRQQLFVSAPKLPAVR"
BASE COUNT          296 a          733 c          717 g          409 t
ORIGIN      
        1 tccttggcgt acaggccgct ctgctggcgc ttgagctcgt cggcctgctg gttcatgccc
       61 gcctggtcga ccgcgaagcg cacccggccg cccttctgca gctcggcgcg gatgctgtcg
      121 gtgatggcac gggtatcgat gtactcgctc gtcttgttgc gcacttcctg caccgtgacc
      181 acgggcaggt tgccgctcgc gatcaccggc gcctggagca tcgagcgcgc catcgactcg
      241 gccatcaatt gcaggtcggt cgagccgtac tcgttggtga cggtctcgac gccgcgcgcg
      301 tcgccatagc gggtctgctc cttggcgcag ccgccgagta ccgccgcggc ggcgagcgcg
      361 accacgatgc gcagtttcat tcctcgctcc tgaggatcag gcggaaatcc accgccttgt
      421 tggtcggcga gatggtgttg atcacgaggt tggagttttc gtagagcatg agcggcttcc
      481 acgcttcttc ctcccaggcg cgcatgcctt cgcggtccat ccagcggaac ttgtacgcga
      541 tgcgcttgac gctgcggctg tcgttgttca ggttggcgtc gatccgcatg aggtcgcccg
      601 cctgcgagca gcgcatctcg gtcgcctgga tcgtggtgcg ccgcccttcg ctcagcacct
      661 tggccttgac gaggtcgtcc atcgagctca ctttggtctc gccgatcggg cagccaaacg
      721 cgtagccggg cttttcgaag cccttcttcg ccgcgcagcc ggccacgagc gcgcttgcgg
      781 ctatcgcggc gatcacgagc gtggtcttca tgtgacctcc ctgtttcaac gcactgcggg
      841 cagtttcggc gcgcttacga aaagctgttg gcgcaacagg cgcaagtcta ccaccgcgta
      901 gcggcccgac acggtggcgg aggcgctgcg cggcccgtcc ggcgtgtgca gcgtgatggt
      961 gtgcacgccg cgcggtacgc gcgcccgggc gatcgacacc tccgacggaa gcgcgcgcca
     1021 cgtgcggtcg tcggcggagg cgagcaggac cgagcttgca gtgaggatgc cggccgcaat
     1081 cgccattgcc gccgactgct cgcggtccga cgcgcgctgc gcctggtatt gcagcgtcgc
     1141 gttcacggtg gagcggatcg tcgcgcgcag catgatcgcc ggcatgtcgt ccatcaggcg
     1201 acgccgcgcc atgagatcga cgctcgtgat gcgcgccgcc gcaaggctgc gcccgtcgag
     1261 cgtcagctcg ctcggcagcg gcgtccacga ggtggcgacc atcaccggga aggcgttggc
     1321 gatcaggacc atccgcgttt cgatgaagat cggcagcagg aattgtcggg actggatcgc
     1381 gggtgcggag ccgctgccca cgatgaaaag cacctccgtc atgccgtcat cgcgcgcgcc
     1441 ggcaacgcgc tcgtcgagcc cgcgcagcgc ttcttcgagc agcggctggc ccggctgcag
     1501 ctcgttcgcc agccggtatc ccggtgcagc caggctcggc tcgcccagcg cctcgtagat
     1561 gaatcccgcg aggtaatgcg acagcgcgct ctggtagccg ttgcgcagcg cgttcacctc
     1621 gggcgtatcg atggtttcga tcgggtagcc gttcagctcc ttgaagctcg tgcgcgcgcc
     1681 ccgctggcgc gccgactctt cgacttccgc gtactgcttc gcccgtgcct ccgcgatctg
     1741 cgcctcgagc tcgtgcgcct gcttgatcgc cacgcgcgcc ccgtcgaagt cacccatcgc
     1801 aaggtgattg agcgccaggt acgtgaggag catcaccttc tcgtagtcgt agcccggata
     1861 ggcgcgcagc ttgtcgctca cgacgtagct cgagacgccg cgcacgaagc tcgatgcctc
     1921 ggcgagcccg cccgcattct gggcttcgat gcgcgcggcg gcgctcttcc acgccttctg
     1981 gctttcctcg tagcggccgg cgagcctctg cagcatgccg agctcgaggt agtagagcag
     2041 atccttgtcg ctcttgcggt tgttcgactc gagcagcctg atcgcgtcgt cgaccgcgcc
     2101 ggtggacgcg cgctcgagcg tgacgtagag ctcggcgtca tagctgcgca ttgcg
//
LOCUS       DASUNT010000627         4828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1043539, whole genome shotgun sequence.
ACCESSION   DASUNT010000627 DASUNT010000000
VERSION     DASUNT010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043539"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2505)
                     /gene="glnE"
                     /locus_tag="VFX94_12640"
     CDS             complement(<1..2505)
                     /gene="glnE"
                     /locus_tag="VFX94_12640"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011325861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEX5769073.1"
                     /translation="MSQSIPHGGQKLALSRFAQRLLAARPELAAELADPQPFSRAEML
                     DALRCRADDELARELRRLRARVLLRVMARDLAGLAALEEVCGTMSDLAEIAIEAALGA
                     EQLMVVGMGKLGGRELNVSSDVDLVFLYRESAEAQERYERAGRRLIRRLSETTAEGFV
                     FRVDMRLRPYGDSGPLACNLEALEQYFITQGREWERYAWLKARAITAPGGGSALRELD
                     AVVRPFVFRKYLDYGAFGALRALHAEVRRDVERRELAEHVKLGPGGIREIEFIVQALQ
                     LVRGGRDPELRVRATLEALRILARKSLLPESAARELSDAYVFLRTLEHRLQYLDDAQR
                     HDLPEDEEDRSRVAAMCGFASWGAFHACWRAVRASVSRHFLDVFAEPSAPPRPWPQHP
                     RLAALRASQRYAALPEESRRRLDRLVPALARAAQGTPDAEATLARAVDLVEAIASRAA
                     YLALLAEHPEALERVARMIGASSWAAEYVTRHPLLLDELLDDRVLYAPPDWVAFSESL
                     KGQLAAAHDDTERRMNILREMHQAQVFRLLAQDLAGLLTVEKLADHLSALADLMLQAC
                     IDEVWHELRGRHREAPRFAVIGYGKLGGKELGYASDLDIIFLYDDDDERAPEVYARLA
                     QRLNTWVSSRTSSGILFETDLELRPSGASGLLVSSIAAFERYQNESAWVWEHQALTRA
                     RYCAGDADVGDKFEAIRQRILTRKRDPKPLKAEILAMRDKLHAAHPNTSGLFDVKHDA
                     GGMIDIEFAVQFLVLGFSSEFPSLTGNLGNIALLSMAADHGLIPAELAERCRNAYREF
                     RRIQHSLRLNGARYARVPRERVAAETEAVRMLWQAVF"
     gene            2532..>4828
                     /locus_tag="VFX94_12645"
     CDS             2532..>4828
                     /locus_tag="VFX94_12645"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HEX5769074.1"
                     /translation="MNSTMKRLLRALEVLAWSAFFALAVLVLTVRFWVLPDIERYREG
                     IVAAMSRGLGMPVRIGAIQAGWLGLRPQVALSDVRLYDAQGREALVLPLIDNVIAWRS
                     LARGEILLHSVAIENPRLSVRRDAAGDLYVAGIRISRGAGGGFGAWVLAHEDISIRGA
                     EIEWLDELRAAPPLVLSDVELRLVNRGRSHWLGLNATVPAELGSTLQARAELQAEDLD
                     PAALSGRAFLELGYTDLAAWRAWVDYPFDVRAGHGALRVWTDLERGKPVSATTDLALA
                     DFRASLADELSPLELASVQGRVQARALADGVELAGRGLAVVLQGGPEVPRTDFSIVWR
                     PQAGGAVTANVIELDSLARVAGALPLPPQLSQAIEELAPRGRLDEPRLEWSGPFDAPA
                     RLGVRMRFSQLALRPSGAIPGFSGLSGSLESTDERGSVALAGRNASVDLPRVFPEPTI
                     ALDSLSGQVDWERGRDGGLAVRIASLTFANAHASGNVYGSYARRDEGPGSIDLSGVLN
                     RADGREVGRYLPHVVPDEVRRWLTRGIVAGEGTNVRLRIRGDLRNFPFVNPATGQFQV
                     TAHIEKGVLDYAADWPRIDNIVGELNFERDRFQLTGRSGTMLGAQLSNVRVAIPSLRG
                     PDRHVQVSGQADGPSADFLKFVHSSPLRKDLGQRLADLKASGRGKLRLKMDIPLAALT
                     NTRVTGEFEFAANEVTVLPGLPAIESASGRVAFTDSGFTLHDARGQVFGGAVAVSGGT
                     GAGGALEVLARGDASFESTRTLFDHP"
BASE COUNT          689 a         1687 c         1714 g          738 t
ORIGIN      
        1 gaacaccgcc tgccacaaca tcctgaccgc ctcggtctcg gccgccactc gctcgcgcgg
       61 cacgcgcgca tagcgcgcgc cgttcagccg caagctgtgc tggatgcgcc ggaactcgcg
      121 gtatgcgttt cggcagcgct ccgcgagctc ggcggggatc aggccgtggt ccgccgccat
      181 gctcagcagc gcgatgttgc cgaggttgcc ggtgagggaa gggaattcgc tcgagaagcc
      241 caggacgaga aactgcaccg cgaattcgat gtcgatcatg ccgcccgcgt cgtgcttgac
      301 gtcgaatagg ccagaggtat tcgggtgcgc cgcatgcagc ttgtctctca ttgcaagaat
      361 ttccgccttc agcggcttcg gatcccgctt tcgggtgaga atccgctgcc ggatggcttc
      421 gaatttgtca cccacgtccg cgtctcccgc gcaatagcgc gcgcgggtga gcgcctggtg
      481 ctcccatacc cacgcgcttt cgttctggta gcgttcgaag gctgcaatcg aggagacgag
      541 cagccctgag gcgccgctcg gcctgagctc caggtcggtc tcaaagagaa tcccggacga
      601 ggtgcggctg cttacccagg tgttcagtcg ctgggcgagg cgcgcataga cttcgggcgc
      661 acgctcgtca tcatcgtcgt acagaaagat gatgtcgaga tcggaggcgt agccgagctc
      721 cttgccgccg agcttgccat agccgatcac cgcgaagcgt ggcgcctcgc ggtgccggcc
      781 gcgcagctcg tgccacacct cgtcgatgca ggcttgaagc atgaggtcgg cgagcgccga
      841 gaggtgatcg gcgagcttct ccaccgtgag cagcccggcg agatcctgcg cgagcaggcg
      901 aaacacctgc gcctggtgca tctcgcgcag gatgttcatg cgccgctcgg tgtcgtcgtg
      961 cgcggccgca agctgccctt tcaggctctc cgagaacgca acccagtccg gcggcgcgta
     1021 cagtacccgg tcgtcgagca gctcgtcgag gagcagcggg tggcgcgtga cgtattcggc
     1081 ggcccagctc gaagcgccga tcatgcgcgc gacgcgctcg agcgcctcgg ggtgctcggc
     1141 gaggagcgcg aggtacgcgg cgcggctcgc gatcgcctcg acgagatcca ctgcccgggc
     1201 aagcgtcgcc tcggcatcgg gcgtcccctg cgcggcacgc gcaagcgccg gaaccaggcg
     1261 atcgagccgg cggcgcgact cctccggcag cgccgcgtag cgctggctcg cgcgcagcgc
     1321 ggcaagccgc gggtgctgcg gccagggccg cggcggtgcg ctgggctcgg caaagacgtc
     1381 gaggaaatgg cgcgacaccg aggcgcgcac ggcgcgccag cacgcgtgga acgcccccca
     1441 ggacgcgaac ccgcacatcg ccgcgacgcg cgagcggtcc tcctcgtcct cgggcaagtc
     1501 gtggcgctgg gcgtcgtcca gatactgcag ccggtgctcg agggtgcgca gaaatacgta
     1561 cgcgtcgctg agctcgcgcg cggcgctttc gggcaaaaga gacttgcgcg caaggatcct
     1621 gagggcctcg agggtcgccc tgacgcgaag ctccgggtcg cggccgccgc gcacgagctg
     1681 cagcgcctgc acgatgaact cgatctcgcg gatgccgccg gggccgagct tgacgtgctc
     1741 ggcgagctcg cggcgctcga catcgcgccg cacctcggcg tgcagcgcgc gcagcgcgcc
     1801 gaacgcgccg tagtcgagat acttgcggaa cacgaacggg cgcacgaccg cgtcgagctc
     1861 gcgcagcgcg ctgccgccgc cgggcgccgt gatggcgcgc gccttgagcc aggcatagcg
     1921 ttcccattcg cgtccctggg tgatgaagta ctgctcgagc gcctccaggt tgcacgcaag
     1981 cgggccggag tcgccgtagg gccgcagccg catgtcgacg cggaacacga agccctcggc
     2041 ggtcgtctcg gagagccgcc ggatcagcct gcgcccggcg cgctcgtagc gctcctgcgc
     2101 ctccgcgctc tcgcggtaca ggaacacgag gtcgacgtcc gaggagacgt tgagctcccg
     2161 cccgccgagc ttccccatcc cgaccaccat gagctgctcg gcgccgagcg ccgcctcgat
     2221 cgcaatctcg gcgagatcgc tcatcgtgcc gcacacctcc tcgagcgcgg cgaggccggc
     2281 gagatcgcgc gccatgacgc gcaggagcac acgcgcgcgc agccggcgca gctcgcgcgc
     2341 gagctcgtca tccgcgcggc accgcagcgc gtcaagcatc tcggcgcggc tgaagggctg
     2401 cgggtcggcg agctcggcgg cgagttccgg ccgcgccgcg agcagccgct gcgcgaagcg
     2461 cgagagcgcg agtttctgcc cgccgtgcgg gatcgattgc gacattggca gtttagaatg
     2521 gccgcctgga cgtgaattcc accatgaagc gactgctgcg ggcgctcgaa gtgctcgcct
     2581 ggtcggcctt tttcgcgctt gccgtgctcg tgctcacggt gcgcttctgg gtgctgcccg
     2641 acatcgaacg ttaccgcgaa ggcatcgttg ccgccatgtc gcgcggcctc ggcatgccgg
     2701 tgcgcatcgg cgccatccag gcggggtggc tcggcctgcg gccgcaggtc gcgctttccg
     2761 acgtgcggct ctacgacgca caggggcgcg aggcgctcgt gctgccgctc atcgacaacg
     2821 tgatcgcctg gcgctcgctc gcgcgcggcg agatcctgct gcacagcgtg gcgatcgaga
     2881 acccgcgcct ttcggtgcgg cgcgacgccg ccggggatct ctacgtggcg gggatcagga
     2941 tctcgcgcgg cgcgggcgga ggcttcggcg cgtgggtgct cgcgcacgag gacatctcca
     3001 tccgcggcgc cgagatcgaa tggctcgatg agctgcgcgc cgctccgccg ctcgtgctgt
     3061 cagacgtgga gctcaggctc gtgaaccgcg gccggtcgca ttggctcggc ctgaacgcga
     3121 ccgtccccgc cgagctcggc agcaccttgc aggcgcgcgc cgagctgcag gccgaagacc
     3181 tggacccggc cgcgctgagc ggccgcgcct tcctggagct cggctacacc gacctcgctg
     3241 cgtggcgcgc ctgggtcgac tatccgttcg acgtccgcgc cgggcacggc gcgttgcgcg
     3301 tctggaccga tctcgagaga ggcaagccgg tttcagccac caccgatctg gcgctcgcgg
     3361 attttcgcgc aagcctcgcc gatgagctct cgccgctcga gctcgcttcg gtgcagggtc
     3421 gggtgcaggc ccgcgcgctc gccgacgggg tggagcttgc cggccgcggc ctcgcggtgg
     3481 tgctgcaagg cgggcccgaa gtcccgagga ccgacttcag catcgtctgg cgtccgcagg
     3541 cgggcggtgc cgtcacggca aacgtcatcg agctcgattc gctcgcccgc gtagccggcg
     3601 cgctgcccct gccgccgcag ctctcccagg cgatcgaaga gctcgcgccg cgcggccgcc
     3661 tggacgagcc gcgcctggag tggagcgggc cgttcgatgc gccggcgagg ctcggcgtgc
     3721 gcatgcgctt ctcgcagctc gcgctgcgcc cgagcggcgc gatcccgggg ttctcgggtc
     3781 tgagcggcag cctggaatcc accgacgagc gcggaagcgt cgcgctcgct gggcgcaacg
     3841 cgagcgtgga tttgccccgc gtctttcccg agcccacgat cgcgctcgac tccctctccg
     3901 gccaggtcga ctgggagcgc ggccgcgacg gcgggctcgc ggtgcgcatc gcctcgctga
     3961 cgttcgcgaa cgctcatgca agcgggaacg tctacggcag ctatgcccgc cgcgacgaag
     4021 ggccggggag catcgacctc tccggagtgc tcaatcgcgc cgacgggcgc gaggtggggc
     4081 gctacctgcc tcacgtcgtt ccggacgagg tgcgccgctg gttgacccgc ggcatcgttg
     4141 ccggcgaggg gacgaacgtg cggctgcgca tccgcggcga cctgcgcaat ttcccgttcg
     4201 tcaatccggc cacggggcag ttccaggtca cggcgcatat cgagaagggc gtgctcgatt
     4261 acgccgcgga ttggccgcgc atcgacaaca tcgtcggcga gttgaacttc gagcgcgacc
     4321 gcttccagct cacggggcga agcggcacca tgttgggcgc gcagctctcc aacgtacgcg
     4381 tcgccatccc gagcctgcgc gggccggacc ggcacgtgca ggtgagcggg caagccgacg
     4441 gcccgagcgc cgacttcctg aagttcgtgc attcctctcc gctgcgcaag gatctcgggc
     4501 agcgcctggc cgatctcaag gcgtctggac gcggaaagct gcgcctcaag atggatatcc
     4561 ctctcgccgc gctcacgaac acgcgggtca cgggggagtt cgagttcgcc gcaaacgagg
     4621 tcacggtgct gcccgggctc ccggccatcg aatcggcttc cgggcgggtg gctttcaccg
     4681 actcgggctt cacgctgcac gatgcgcgcg ggcaggtgtt cgggggcgcg gtcgccgtct
     4741 cgggcggcac gggagccggg ggagccttgg aggtgctcgc gcgcggcgac gcttccttcg
     4801 agtccacacg cacgcttttc gatcaccc
//
LOCUS       DASUNT010000628         2037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1131792, whole genome shotgun sequence.
ACCESSION   DASUNT010000628 DASUNT010000000
VERSION     DASUNT010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131792"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VFX94_12650"
     CDS             <1..791
                     /locus_tag="VFX94_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ni,Fe-hydrogenase III large subunit"
                     /protein_id="HEX5769075.1"
                     /translation="IALELERVANHLGDLGYLGNDVALSFGFFQFWRLKEALLRMHGE
                     AFGHRYLMDLVIPGGVARDLVNKRRLLEVIAYIKSEVDILRGIYDEHAGVQDRFIMAG
                     QVLPELAARLGLTGPAARASGQALDLRADFPMPPYETLGVRKAGHLRGDVAARVTVRF
                     EELAESIRMIEELVERLPPGGLQAPWKAPEAGSRGCGWIEGWRGEVFVALEAGANGAL
                     ERVHAHDPSWQNWPALERAVLGNIVPDFPLINKSFNLSYSGHDL"
     gene            794..1318
                     /locus_tag="VFX94_12655"
     CDS             794..1318
                     /locus_tag="VFX94_12655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006027183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX5769076.1"
                     /translation="MYTILKQIARTGIVTEPAPRPDSSLQPLEQRLSEAVLRHFGRAL
                     AIRHVDAGSCNGCELEIHACNSPYYNLERLGIRFVASPRHADLLLVTGPVSRNMETAL
                     RRTYEATPEPKLVVAIGDCGCTGGIFGESYASCGRVSNVIPVDVAVPGCPPEPVAIIR
                     GILAAVARKKSTAR"
     gene            complement(1315..1710)
                     /locus_tag="VFX94_12660"
     CDS             complement(1315..1710)
                     /locus_tag="VFX94_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769077.1"
                     /translation="MRAALLVIAGLALIGYVAVAWVLPSMAGSEVRAAAQALVSGADS
                     AKQEVSAKAEKSGNLAGAGSGVKLVEKDDPKYGKMKWIVADNGAIRAWNEKNALEVTF
                     TPSLQGGKTTWNCKGYPVDAMPAICGGKG"
     gene            1772..>2037
                     /locus_tag="VFX94_12665"
     CDS             1772..>2037
                     /locus_tag="VFX94_12665"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX5769078.1"
                     /translation="MARHYRLWRAPARVCAAGRLTFFYGMVSVRLRISGRVQGVGFRY
                     AMQAEAERRGLGGWVRNRRDGTVEALVHGDAAALEALVEAEGAAT"
BASE COUNT          323 a          679 c          677 g          358 t
ORIGIN      
        1 caatcgcgct agagctcgag cgcgtcgcga accacctcgg cgatctcggc tacctcggca
       61 atgacgttgc actctcgttc ggcttcttcc agttctggcg actgaaggaa gcgctgcttc
      121 gcatgcatgg cgaagctttc ggccaccgtt acctgatgga tctcgtgata cccggggggg
      181 tcgcaagaga cctagtaaat aaacgacgtt tgttggaagt cattgcttac atcaagagtg
      241 aggtagacat cctgcgcggc atttacgacg aacatgccgg ggtccaggac cgcttcatca
      301 tggccggcca ggtcctgccc gagctggccg cacggctcgg cctcaccggg ccggcggcac
      361 gcgccagtgg gcaggcgctc gacctgcgcg ccgactttcc catgccgccg tacgaaacgc
      421 tcggcgtgcg caaggcgggc cacctgcgag gcgatgtagc tgcgcgcgtc acggtgcgct
      481 tcgaagagct tgccgagtcg atccggatga tcgaggagct cgtcgagcgg ctcccgcccg
      541 gcggccttca ggcgccctgg aaagcgcccg aggcgggcag ccgcggctgc ggctggatcg
      601 aaggttggcg gggcgaggtg ttcgtcgcgc tcgaagccgg agcgaacggc gccctggagc
      661 gggtgcatgc gcacgacccc tcctggcaga actggccggc gctcgagcgc gcggtgctcg
      721 gcaacatcgt cccggacttc ccgctgatca acaagtcgtt caacctgagc tacagcggac
      781 acgacctcta ggcatgtaca cgatcctgaa gcagattgcc cgcacgggca ttgtcaccga
      841 gccagcgccg cggccggata gctcgctgca gccgttggag cagcgcctga gcgaggcggt
      901 gctcaggcat ttcggccgcg cgctcgccat ccgccatgtc gacgcgggct cgtgcaacgg
      961 ctgcgagctc gagatccacg cctgcaacag tccctactac aacctcgagc ggctcggcat
     1021 ccgcttcgtg gcgagcccgc gccatgcgga ccttctgctc gtgaccggcc cggtgtcgcg
     1081 caacatggaa accgccctgc ggcgtaccta cgaggcgacg cccgagccga agctcgtggt
     1141 cgctatcggg gactgcgggt gcacaggggg gatattcggc gaaagctacg cgagctgcgg
     1201 cagggtgtcg aacgtaatcc cggtcgacgt ggcggttccg ggctgcccgc ccgagccggt
     1261 cgcgatcatc cgcggaattc tcgccgcggt cgcgcggaaa aaaagtacgg cgcgctagcc
     1321 cttgccgccg cagatggccg gcatcgcgtc caccgggtag cccttgcagt tccaggtggt
     1381 ctttccgccc tgcagcgagg gagtgaaggt cacctcgagc gcgttctttt cgttccaggc
     1441 gcggatcgcg ccgttatcgg ccacgatcca tttcatcttg ccgtacttgg ggtcgtcctt
     1501 ctcgacgagc ttgacgccgc tgccggcgcc ggcgaggttg cccgacttct cggcctttgc
     1561 cgatacttcc tgcttcgccg aatcggcccc gctgacgaga gcctgcgccg cagcccttac
     1621 ctccgagccg gccatcgagg gcaacaccca ggcaaccgcg acgtatccga tcagggcgag
     1681 accggcgatc actagcaacg cagcacgcat ttggtttctc cctttgttgc taccgcgagt
     1741 actttttctc cgtgcttaag aacagcttac catggcgcgt cattaccggc tttggcgcgc
     1801 cccggcgcgt gtctgcgccg ccggccgcct gaccttcttt tacggaatgg tgagcgttcg
     1861 gctgcggatc agcgggcgag tccagggcgt cgggttccgc tatgcgatgc aagccgaagc
     1921 cgagcggcgc ggcctcggcg gctgggtgcg taaccggcgc gatggcaccg tggaggctct
     1981 ggtgcacgga gacgccgccg cgctcgaggc gctcgtcgag gccgagggcg cggcgac
//
LOCUS       DASUNT010000629         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1219954, whole genome shotgun sequence.
ACCESSION   DASUNT010000629 DASUNT010000000
VERSION     DASUNT010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219954"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VFX94_12670"
     CDS             <1..496
                     /locus_tag="VFX94_12670"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465596.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX5769079.1"
                     /translation="KAGVIETSFREETETDLFGEQTVLCGGCVELVKAGFDTLVEAGY
                     APEMAYFECLHELKLIVDLMYEGGIGTMNYSISNNAEYGEYVTGPKIIDEGVRRRMKE
                     ALHRIQNGEYAKEFVLENRAGAPGLLSQRRMTAELPIEKVGARLREMMPWIKRNRLVD
                     TSKN"
     gene            493..1167
                     /locus_tag="VFX94_12675"
     CDS             493..1167
                     /locus_tag="VFX94_12675"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEX5769080.1"
                     /translation="MKRRPYPHPFIAREGWLFVAIALAASLLAGFVAGWWWSIPLWLA
                     AVFVLQFFRDPPRQVPDAPGAVLSAADGRVVEVSRAQDPYVKRDALKVSVFMNVFNVH
                     SNRSPVDGVVKERWYFPGAFVNAALDKASLSNERNALWLRTPEGHDVTCVQVAGLIAR
                     RILCYVGAGAELERGERFGFIRFGSRVDHYLPLDAEVKAAIGDKVYAAQTVLAWLRTP
                     TTTRSS"
     gene            1131..1943
                     /locus_tag="VFX94_12680"
     CDS             1131..1943
                     /locus_tag="VFX94_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HEX5769081.1"
                     /translation="MAENPDYDPIELAEPHAGPPPAGPGGLRRRGIYLLPNLFTLGVL
                     FCGFFAIVQAMNGRFEVGCIAIFVAMVLDGMDGRVARWTNTQSEFGAQFDSIADMVAF
                     GAAPALMAYTWVLKDIQLFGLGWIGAFIYCAGAGIRLARFNANIAVVDKRYFQGLPSP
                     SGAAVVAGFIWVLVDFGFGPREHPWLAWLAWAVAVFAGFTMVSNVPFWSFKEVNWRKR
                     VPLWVILAAVVGISVVASRPSLVLFALIMAYAISGYVMWAMGHRVRPVLPEE"
     gene            complement(1894..>2033)
                     /locus_tag="VFX94_12685"
     CDS             complement(1894..>2033)
                     /locus_tag="VFX94_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEX5769082.1"
                     /translation="PLLERVTHPVAVDPDAKLEKTARARGWRVITLREAPAARGDPSP
                     T"
BASE COUNT          298 a          673 c          701 g          361 t
ORIGIN      
        1 caaggccggc gtcatcgaga cgagctttcg cgaggaaacc gagaccgatc tcttcggcga
       61 gcagaccgtg ctctgcggcg gctgcgtcga gctcgtcaag gccgggttcg atacgctggt
      121 cgaggcgggc tacgcgccgg agatggcgta cttcgagtgc ctgcacgagc tcaagctcat
      181 cgtcgacctg atgtacgagg gcggcatcgg cacgatgaac tactcgatct ccaacaacgc
      241 ggagtacggc gagtatgtca cggggccgaa gatcatcgac gagggcgtga ggcggcgcat
      301 gaaggaggcg ctgcaccgca tccagaacgg cgagtacgcc aaggagttcg ttctcgagaa
      361 ccgcgccgga gcgccgggcc tgctcagcca gcggcgcatg accgccgagc tgccaatcga
      421 gaaagtgggc gcgcggctgc gcgagatgat gccgtggatc aaacgcaacc gcctggtcga
      481 cacttcgaag aattgaagcg acggccttac ccgcatccgt tcatcgcgcg cgaggggtgg
      541 ctgttcgtcg ccatcgcgct ggcggcgtct cttctggccg gcttcgtggc cggctggtgg
      601 tggtcgatcc cgttgtggct cgccgcggtg ttcgtcctcc agttcttccg cgacccgccg
      661 cgccaggtgc ctgacgcccc gggcgccgtg ctgtcggcgg ccgacggccg cgtggtcgag
      721 gtgagccgcg cgcaggatcc ctacgtgaag cgcgacgcgc tcaaggtcag cgtgttcatg
      781 aacgtgttca acgtgcactc gaaccgctcg ccggtcgatg gcgtggtgaa ggagcgctgg
      841 tactttcccg gcgctttcgt caacgccgcg ctcgacaagg cgtcgctctc gaacgagcgc
      901 aatgcgctgt ggctgcgcac gccggaagga cacgacgtca cctgcgtcca ggtcgccggg
      961 ctgattgcgc ggcgcatcct gtgctacgtt ggcgccggcg ccgagctcga gcggggcgag
     1021 cgcttcggct tcatccgctt cgggtcacgc gtcgatcact acctgccgct cgacgccgag
     1081 gtgaaggcag cgatcggcga caaggtgtac gccgcacaga ccgtgctcgc atggctgaga
     1141 accccgacta cgacccgatc gagctagccg agccgcacgc cgggccgccg cccgccggcc
     1201 cgggcgggct gcgccggcgc ggcatctacc tgctgcccaa cctcttcacg ctcggggtgc
     1261 tcttctgcgg gttcttcgcc atcgtgcagg ccatgaacgg gcgcttcgag gtcggctgca
     1321 tcgcgatctt cgtcgccatg gtgctcgacg gcatggacgg gcgcgtcgcg cgctggacca
     1381 acacccagag cgagttcggc gcgcagttcg actcgatcgc cgacatggtg gcgttcggcg
     1441 cggcccccgc cctcatggcc tacacctggg tgctgaagga catccagctc ttcggcctgg
     1501 gctggatcgg cgccttcatc tactgcgcgg gcgccggcat ccgcctcgcg cgtttcaacg
     1561 ccaacatcgc ggtggtggac aagcgctact tccagggctt gccgagcccc tcgggcgccg
     1621 cggtggtcgc gggcttcatc tgggtgctgg tcgacttcgg cttcggcccg cgcgagcacc
     1681 cctggctcgc atggcttgcc tgggcggtag cggtcttcgc cggcttcacc atggtgtcga
     1741 acgtgccctt ctggagtttc aaggaagtga actggaggaa gcgcgtgccg ctgtgggtca
     1801 tccttgcggc ggtggtgggc atctccgtgg tggcctcgcg accgtccctc gtgctcttcg
     1861 cgctcatcat ggcgtacgcc atctcgggct acgtcatgtg ggcgatgggt caccgcgtgc
     1921 ggccggtgct tcccgaagag taatcacgcg ccagccgcgc gcacgcgcag ttttctccag
     1981 tttcgcatcg ggatcgacgg cgaccggatg agtgacccgc tcgagcagcg gca
//
LOCUS       DASUNT010000630         3903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_519717, whole genome shotgun sequence.
ACCESSION   DASUNT010000630 DASUNT010000000
VERSION     DASUNT010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519717"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VFX94_12690"
     CDS             <1..950
                     /locus_tag="VFX94_12690"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5769083.1"
                     /translation="VNVGFAEPVSRNVRRELERLPGVAQVEVSRAIPVRLRAGYRSYR
                     TGITGVEDEATLSRIMDADLREAKPAPGGVLLTTRLAERLGVRPGDSVSAELLEGKRV
                     RAELRVTGTVREMAGMNAWMRLEDLNRLALEGPVVSSAALTVERAQENALLERLKQMP
                     AAAVVIVNRSLLETFRETTGRNVLFFTTVLSIFAATIAVGVVYNNARIQLAERAWELA
                     SLRVLGFTRREVSVLLLGELAVEIALAIPLGFLAGFWLAGLLLALMRVEMMEFPLVIF
                     PTTYLYAGAVVLAAGVVSALIVRNRIDNLDLVAVLKTRE"
     gene            947..2131
                     /locus_tag="VFX94_12695"
     CDS             947..2131
                     /locus_tag="VFX94_12695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5769084.1"
                     /translation="MTRRNKTALAAILIALALLVGWALRPQAVAVETAEVTKGAFEQT
                     VSDDGQTRVRDRYVIAAPLAGHVERIALEVGDPVKQGQIVAELTPTAPAFLDARTQRE
                     LRERIGAAEAQLARARAELLKTQAQRDQASADRERQARLSKEGFLSKNALEQAELALR
                     TSERAVEAARFAEEAARHDAAQARAAMTRAESGAPPTKWPVTSPVSGVVLKMVQKSEG
                     AVGLGAPLLEVADPRTLEAVVDVLSQEAVAVRPGMPARLELGQGVPPIPARVRLVEPA
                     AFTKVSALGVEEQRVNVVLDFQGPLDRLQTIGDGFRVEAHIVVFREEAAVKAPVGALF
                     REGAGWAAFVVEGERARKRAVKLARRNNLEAMIEDGLKPGERVVVYPSDALKDGSRVR
                     PG"
     gene            complement(2100..2498)
                     /locus_tag="VFX94_12700"
     CDS             complement(2100..2498)
                     /locus_tag="VFX94_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769085.1"
                     /translation="MDTPSVAVLLEIDHEYREKARAGSLPMIAPRRLNPEGKAWLPVL
                     HRRYAPWHFTALFSNTERAHLLHRVYDWVVIFYEGADGTGQATVVTERRGALTGERVV
                     RGREPECARYYRGELVPPAMATPAERASRP"
     gene            2645..3163
                     /locus_tag="VFX94_12705"
     CDS             2645..3163
                     /locus_tag="VFX94_12705"
                     /inference="COORDINATES: protein motif:HMM:NF023682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermostable hemolysin"
                     /protein_id="HEX5769086.1"
                     /translation="MSVLIVDTRSELERFIAERFRIAYGAQVKHFCRQLLGMRDADGA
                     WQAAAGFSPAADGPLYLEHYLDRPVEAVLADALGTRVPRAQIVEVGNLASAAKGFGSR
                     FLPTLRRHLLERDFRWVVFTATRQVRAILEHLAFEARALAPAAPSRLPDGGVGWGTYY
                     AHDPNVMAGYIA"
     gene            3160..>3903
                     /locus_tag="VFX94_12710"
     CDS             3160..>3903
                     /locus_tag="VFX94_12710"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5769087.1"
                     /translation="MTAVGPGSLDARVARMAKALAGARPRPRVAGLAADNGVDWVVAD
                     LAAERAGVVLVPLPAFFTAGQLEHVVAASGMDSVLGARLAGFEAVGELEGLRWSHRPC
                     ETPELPRGTSKLTFTSGTTGAPKGVALRSAQQWAVARSIAGVARALALRRHLCLLPLS
                     VLLENVGGVYAALAAEMQFCVPSLAEVGMRGSSSFDADACLAAIEHWQAESVILLPQM
                     LAALASAVERGAVAPRSLKFAAVGGARVSP"
BASE COUNT          553 a         1345 c         1398 g          607 t
ORIGIN      
        1 atgtgaacgt cggcttcgcc gagccggtat cgcgcaatgt gcgccgcgag ctcgagcggc
       61 tgccgggcgt ggcgcaggtc gaggtgagcc gcgcgatacc ggtgcgcctg cgcgccggct
      121 accgcagcta ccgcaccggc atcaccggcg tcgaggacga ggcgacgctc tcgcgcatca
      181 tggacgcgga cctgcgcgaa gcgaagcccg cgccgggcgg ggtcctcctc accacgcggc
      241 ttgccgagcg gctcggcgtg cgcccgggag acagcgtgag cgccgagctg ctcgaaggaa
      301 agcgggtgcg tgcggagctg cgcgtcaccg gcaccgtacg cgagatggcc ggcatgaacg
      361 catggatgcg cctggaggac ctgaaccgcc tcgcgctcga gggcccggtg gtttcctccg
      421 ccgccctgac ggtcgagcgt gcgcaggaga acgcgcttct cgagcgcctc aagcagatgc
      481 ccgcggccgc ggtggtcatc gtcaaccgca gcctgctcga aaccttccgc gaaaccaccg
      541 ggcgcaacgt gctcttcttc accacggttc tcagtatctt cgccgccacc atcgcggtgg
      601 gcgtggtcta caacaacgcg cgcatccagc tggctgagcg tgcctgggag ctcgccagtc
      661 tgcgcgtgct cggcttcaca cgccgcgagg tatccgtgct gctcctgggc gagctcgcgg
      721 tggagatcgc gctcgcaatc ccgctcggct tcctcgccgg cttctggctc gcggggctgc
      781 tcctcgccct catgcgggtc gaaatgatgg aattcccgct cgtcatcttt ccgaccacct
      841 atttgtacgc cggcgcggtg gtgctcgccg cgggcgtggt gagcgcgctc atcgtgcgca
      901 atcgcatcga taatctggat ctcgtcgccg tgctcaagac ccgcgaatga cccgacgcaa
      961 caagaccgcc ctcgccgcca tcctcatcgc actcgctctt ctggtgggct gggcgctgcg
     1021 cccgcaagcg gtggccgtgg agacggccga agtcacgaag ggagcattcg aacagacggt
     1081 ttcggatgac ggccagacgc gcgtgcgcga ccgctacgtg atcgccgcgc cgctcgccgg
     1141 acacgtagag cgcatcgcgc tcgaggtggg cgacccggtg aagcaaggac agattgtcgc
     1201 cgagcttacg ccgacggcgc ccgcgttcct cgatgcacgc acgcagcgcg agctgcgcga
     1261 gcgcatcggc gccgccgagg cgcagctcgc gcgcgcccgc gccgagctgc tcaagacgca
     1321 ggcgcagcgc gaccaggcga gcgccgaccg cgagcgtcag gcgcggctct ccaaggaagg
     1381 cttcctttcg aagaacgccc tggagcaggc cgagctcgcc ctgcgcacct ccgagcgtgc
     1441 ggtcgaggcg gcgcgctttg ccgaggaagc cgcgcgccac gatgcggcgc aggcgcgcgc
     1501 cgccatgacg cgcgccgaaa gcggcgcgcc gccgacgaag tggccggtga cctcgcccgt
     1561 gtccggcgtg gtgctcaaga tggtgcagaa gagtgagggc gcggtcggtc tcggcgcgcc
     1621 gcttctcgag gtcgccgacc cgcgcacgct cgaagccgtg gtcgacgtgc tctcgcagga
     1681 ggcggtcgcg gtgcggcccg gcatgccggc gcgccttgag ctcggccagg gcgtgccgcc
     1741 gatccccgcg cgcgtgcgcc tcgtcgagcc ggccgcgttc acgaaggtct cggcgctcgg
     1801 cgtcgaggag cagcgcgtga acgtggtgct ggacttccag gggccgctcg acaggctgca
     1861 gacgatcggc gacggctttc gcgtcgaggc gcacatcgtc gtgttccgcg aggaagccgc
     1921 ggtcaaggcg ccggtcggcg cgctattccg cgagggcgcg ggctgggccg cgttcgtagt
     1981 cgaaggcgag cgcgcgcgga agcgcgccgt gaagctcgcg cggcgcaaca acctcgaagc
     2041 gatgatcgag gacggcttga agcccggcga gcgtgttgtc gtctacccgt ccgacgcgct
     2101 taaggacggc tcgcgcgttc ggccggggta gccatcgccg gcggaacgag ctcgccgcgg
     2161 tagtagcgcg cgcattcggg ctcgcgtccg cgtaccacgc gctcgccggt gagcgcgccg
     2221 cggcgctcgg tgaccacggt cgcctggccg gtgccgtcgg caccctcgta aaagatcacg
     2281 acccagtcgt acacgcggtg cagtagatgg gcgcgctcgg tattggagaa aagcgcggtg
     2341 aagtgccatg gcgcgtaacg cctgtgcagc accggcagcc aggccttgcc ctcgggattt
     2401 agccggcgcg gcgcgatcat cggcagcgag ccggcgcgcg ccttttcgcg gtactcgtgg
     2461 tcgatctcga ggagaacggc cacgcttggc gtatccatgc gtttagcgta ggccgaccag
     2521 cgcgacgaac gttgacgcag atcaaacgtc ctgagacaaa gttcacgtgc gcataggccg
     2581 gccggacccc ggcgcgcgtt cgttaatggt cggttaatgc cgcgtgtcta cgatcgcgcc
     2641 gcttatgagc gtactcatcg tcgacacacg aagcgagctc gagcgtttca tcgccgagcg
     2701 tttccggatc gcgtacggcg cgcaggtgaa gcatttctgc aggcaactgc tcggcatgcg
     2761 cgatgcggat ggtgcctggc aggcggccgc ggggtttagc ccggcagccg acggcccgct
     2821 ctacctcgag cactacctgg atcggccagt ggaggccgta ttggcggacg cgctcggaac
     2881 gcgcgtgccg cgtgcgcaga tcgtcgaagt ggggaacctg gcttccgcgg ccaaaggctt
     2941 cggctcccgc tttcttccga cgctgcgccg ccatctgctc gagcgcgact tccgctgggt
     3001 ggttttcacg gccacgcgcc aagtgcgcgc catcctcgag cacttggcgt tcgaggcgcg
     3061 cgcgctggcg ccggcggcgc cgagccgctt gcccgatggc ggcgtcggct ggggcactta
     3121 ctacgcgcac gatccgaacg taatggcggg ctatatcgca tgaccgcggt cggcccgggc
     3181 tcgctcgacg ctcgcgtggc gcgcatggcg aaggccctcg ccggggcgcg gccgcggccg
     3241 cgcgttgctg gcctcgcggc cgacaacggc gtcgactggg tggtcgccga cctcgccgca
     3301 gagagagcgg gcgtggtgct cgtgccgctt ccggcgttct tcaccgcggg acagctcgag
     3361 cacgtggtcg cggcgagcgg catggacagc gtgctcggcg cgcgtcttgc gggcttcgaa
     3421 gcggtgggcg aactcgaagg actacgctgg tcgcaccggc catgcgagac gcccgagcta
     3481 ccgcgcggca cgtcgaagct caccttcacc tcgggaacca cgggggcgcc gaaaggcgtt
     3541 gcgctgcgat cagctcagca gtgggccgtg gcgcgctcga tcgccggggt tgcgcgcgcc
     3601 ctcgcacttc ggcggcatct ttgcctgctg cccctctcgg tgctgctcga gaacgtcgga
     3661 ggggtctacg ccgcgctcgc cgcggagatg caattctgcg ttccttcgct cgccgaagtc
     3721 ggcatgcgcg gctcgtcgag cttcgacgcc gacgcctgcc tcgccgcgat cgagcactgg
     3781 caggccgaaa gcgtgatcct gcttccgcag atgctcgccg cgctcgcgag cgccgtcgag
     3841 cgcggcgccg tcgcgccgcg ctcccttaag ttcgccgccg tgggtggagc gcgcgtttcg
     3901 ccg
//
LOCUS       DASUNT010000631         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1308520, whole genome shotgun sequence.
ACCESSION   DASUNT010000631 DASUNT010000000
VERSION     DASUNT010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308520"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFX94_12715"
     CDS             <1..807
                     /locus_tag="VFX94_12715"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5769088.1"
                     /translation="VDKMIKKNQALADVHESVVGACLMTDLKEFSSVFQTRTPAEVVR
                     LLNNYFDVLFPVVRGAGGHPIDVLGDAMLALWYAASPTPAVRENACLAALQLAEAAGR
                     FRESGSNQVFHTRIGIEYGEMSLSGVGAFFHREFRPVGAPVNAAARLQELAKRLGTQV
                     LVTSSVLAGLKRFLARDVGHFRLRGFDDHVHVYELMGERDHATPAQLDLCEKFKLALA
                     SYQAGKGDEARARFEEILAIKAYERDGPSIFFRQKCASHSYPVQGALVQE"
     gene            1004..>2081
                     /locus_tag="VFX94_12720"
     CDS             1004..>2081
                     /locus_tag="VFX94_12720"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX5769089.1"
                     /translation="MAQVVSLQGQVEIQRAGTTSWMRVERLDTRLCEGDRLRSGSLSR
                     AALLVSGQALLRVDQNSLLGLRASPDEIRVEFDSGRVYSISRFPRRFRILTPFVNAGV
                     EGTEFMVTQGRDQAEIAVFEGRVATEDRVGEPGARQLLQSGQAAGFARGTAPAVRALV
                     NPTDAVQWALYYPPLDSEPTSAGRLLRLGRVDEAEREIQSALAAKPGDAEALALGAMI
                     RVVKNDKQAALDLAQQAVSRDASSVRARLALSYAQQAHFRLDEALASATEAVKLQPAN
                     AVAQARRAELLLSMGRIQDAEAAAGEAVRADPADSRARTVLGFAQLARLDTTAARAQL
                     ERAAALDPSDPLPRLGLGLAAIKEG"
BASE COUNT          339 a          688 c          736 g          318 t
ORIGIN      
        1 gtcgacaaaa tgatcaaaaa gaaccaggcg ctcgccgacg tgcacgaatc cgtggtcggc
       61 gcctgcctga tgaccgacct caaggagttc tccagcgttt tccagacgcg cactccggcc
      121 gaggtcgtgc gcctgctgaa caattacttc gatgtgcttt tcccggtggt gcgtggcgca
      181 ggcggacacc cgatcgatgt ccttggcgac gccatgctcg cgctgtggta cgcggcgagc
      241 ccgactcccg cggtgcgcga gaatgcatgc cttgccgcct tgcagctcgc ggaggcggcc
      301 ggacgcttcc gcgagtccgg gtccaaccag gtattccata cccgcatcgg catcgagtac
      361 ggggaaatga gcctgagcgg cgtcggcgcg ttctttcacc gcgagttccg cccggtgggc
      421 gccccggtga acgcggccgc ccggctccag gagcttgcca agaggctcgg gacccaggtg
      481 ctggtcacga gctcggtgct tgcagggctg aagcgcttcc tcgcccgcga cgtaggccac
      541 ttcaggctgc gcggtttcga cgatcacgtc cacgtctacg agctgatggg ggagcgcgac
      601 cacgcgacgc cggcgcagct cgatctatgc gagaaattca agctcgccct ggcaagctat
      661 caggccggga agggcgatga ggcgagagcg cgcttcgagg agatcctcgc aatcaaggcc
      721 tacgagcgcg acggtccgtc gatattcttc cggcagaagt gcgccagtca cagctatcct
      781 gtgcagggcg cactcgtgca ggagtgagcg gcgcagcaag ctagttgccg ggccagcacc
      841 cggcgggtat cgtacaagca ggcagcggac gctctcaaaa agggacgagg catcgatggt
      901 gccgctgaca agaactcgaa acagctctct gctgctggtt ctggcggcgg gcgcgttcgc
      961 ttttgcgccc gcgcccaggg cccaggaatc ctgcgcgccg gtcgtggcgc aagtcgtctc
     1021 gctgcaaggc caggtggaga ttcagcgtgc cggcacgacg agctggatgc gcgtcgagcg
     1081 gctcgatacc cggctgtgcg aaggcgaccg gctgcgctcg ggctcgctta gccgcgcggc
     1141 gctgctcgtg tccggccagg cgctgctgcg cgtcgatcag aacagcttgc tcgggctgcg
     1201 cgcgtcgccg gacgaaatac gcgtcgagtt cgattccggc cgggtgtact cgatctcgcg
     1261 ctttccgcgc cgcttccgca tcctcacgcc gtttgtgaac gcgggcgtcg aaggcacgga
     1321 gttcatggtc acccagggca gagaccaggc cgagatcgcg gtgttcgagg gacgcgtcgc
     1381 caccgaggac cgcgtcggcg agccgggagc gcggcagctc ctgcagagcg gacaggcggc
     1441 cggcttcgcg cgcggcacgg cgccggccgt gcgcgcgctc gtgaatccca ccgacgccgt
     1501 gcaatgggcg ttgtactacc cgccgctcga ctccgagccg acgagcgcag gcaggctgct
     1561 gcgccttgga cgcgtggacg aggcggagcg cgaaattcag tcggcattgg cggcgaagcc
     1621 cggggacgcg gaggcgcttg cgctcggcgc gatgatccgg gtggtcaaga acgacaagca
     1681 ggcggcgctc gacctggccc agcaggccgt aagccgggat gcgagctccg tacgggcacg
     1741 gctggcgctt tcctatgcgc aacaggcgca cttcaggctt gacgaggcgc tcgccagcgc
     1801 aactgaagcg gtcaagctgc agccggcaaa cgcggtggca caggcccgac gcgccgagct
     1861 gctgctctcc atgggacgca tccaagacgc tgaagcggcg gcaggcgaag cggtgcgcgc
     1921 cgatccggcg gacagccggg cccgcacggt gctcggcttc gcgcagctcg cgcgcctcga
     1981 caccacggcg gcgcgcgctc aactcgagcg ggcggcggcg ctcgacccgt ccgatccctt
     2041 gccgcgtctg gggctgggcc tcgccgccat caaggagggc a
//
LOCUS       DASUNT010000632         2873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_781520, whole genome shotgun sequence.
ACCESSION   DASUNT010000632 DASUNT010000000
VERSION     DASUNT010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781520"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..2290)
                     /locus_tag="VFX94_12725"
     CDS             complement(44..2290)
                     /locus_tag="VFX94_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX5769090.1"
                     /translation="MRPTSKTGTPLPARLAGLLREARWLLLIAAALYLLLIFATFQRS
                     DPGWSHSATDAVARNAGGAFGAWLADLLLYLFGVSAYWWAALCAYIVVWGFRRLDGTR
                     LAVSLGGFALLIASSAALESLRLHSLPAELPLAPGGVIGEVLAGPLSGAFGFTGATLA
                     LLTFAAIGFSLFVGVSWLTIFEAVGTGLEKTYGGGRAAWERRRDRKLGTRARAQREAV
                     VEIEKRREDDHPPLRIEPALAEIRKSERAQKERQAPLFEHLPDTPLPPLKLLDEPAGG
                     GETIAAETLEFTSRLIEKKLSDFGVAVKVLAAYPGPVITRYEIEPAVGVKGSQIVNLV
                     KDLARALSVVSIRVVETIPGKSCMGLEIPNPHRQVVRLSEILGSQAYHGLHAPLVLAL
                     GKDIAGHPVVADLARMPHLLVAGTTGSGKSVALNAMILSLLYKSELRQVRLILIDPKM
                     LELSVYQDIPHLLAPVVTDMKQATNALAWCVAEMDRRYKLMNWIGARNLSGYNHKVAE
                     RKIEDPFAEKTGEAQAKPEILQPLPHIVVVIDELADLMMVVGKKVEELIARLAGKARA
                     AGIHLVLATQRPSVDVITGLIKANIPTRIAFQVSSKVDSRTILDQSGAETLLGAGDML
                     YLPPGTGLPQRVHGAYVADHEVHKVVEYLKSLAKPDYVDEVLESAETEELAVDGVPAG
                     EKDPLYDQAVDIVLRTRRPSISLVQRHLRIGYNRAARLIEDMERAGMVSPMQSNGNRE
                     VLVPAKAE"
     gene            complement(2312..>2873)
                     /locus_tag="VFX94_12730"
     CDS             complement(2312..>2873)
                     /locus_tag="VFX94_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5769091.1"
                     /translation="PRSTTIMAGGDPTDSLYIVLSGRLKVMMSDSEGKEVILAILGPG
                     EFFGEMGLIDDEPRSATVVTIEPCELLSIAKRDFKKAMAENWQMTEAVMRGLVKRLRE
                     ADRKIGSLALLDVYGRVARLLLDMAENVDGEKVVTKRLPKQDIAKMIGASREMVSRVM
                     KDLQTGGYIEMRGSSIVLRDTIMLPE"
BASE COUNT          479 a          961 c          972 g          461 t
ORIGIN      
        1 gagcaacagg agaactgcga acaggaatct catcggagag aaactactcc gccttggcgg
       61 gaaccaatac ctcgcggttg ccgttggact gcatcggaga gaccatgccg gcgcgctcca
      121 tgtcctcgat gaggcgcgca gcgcggttgt agccgatgcg caagtgccgc tgcacgagcg
      181 atatcgaagg gcgccgggtg cgcagcacga tgtccaccgc ctggtcgtac agcgggtcct
      241 tctcgccggc aggcacgccg tcgactgcaa gttcctccgt ttcggcggac tccagcactt
      301 cgtcgacgta gtccggcttc gccagcgact tcaggtactc caccaccttg tggacttcgt
      361 ggtcggcgac gtaggcgccg tgcacgcgct gcgggagccc ggtgcctggc gggaggtaca
      421 gcatgtcgcc ggcgccgagc agcgtctcgg cgcccgactg gtcgagaatg gtgcgcgaat
      481 cgaccttgga ggagacctgg aaggcgatgc gcgtggggat gttcgccttg atgagcccgg
      541 tgatcacgtc caccgacggg cgctgggtcg cgagcacgag gtggatcccc gcggcgcgcg
      601 ccttgccggc gagccgggcg atcagctcct ccactttctt ccccaccacc atcatcaggt
      661 cggcgagctc gtcgatcacc acgacgatat gcggcagcgg ctgcaggatc tccggcttcg
      721 cctgcgcctc gcccgtcttc tcggcaaacg ggtcctcgat cttgcgctcg gccaccttgt
      781 ggttgtagcc ggagaggttg cgcgcgccga tccagttcat gagcttgtag cgccggtcca
      841 tctccgccac gcaccaggcg agcgcattgg tcgcctgctt catgtccgtg accaccggcg
      901 cgagcaggtg cgggatgtcc tggtagaccg agagctcgag catcttcggg tcgatcagga
      961 tgaggcgcac ctggcgcagc tccgacttgt agaggagcga caggatcatc gcgttcagcg
     1021 cgaccgactt gcccgatcct gtcgtgccgg cgacgaggag gtgcggcatg cgcgcgaggt
     1081 ccgcaacgac cgggtggccg gcgatgtcct tgccgagcgc gagaacgagc ggcgcgtgca
     1141 ggccatggta ggcctgcgag ccgaggatct cggacaggcg caccacctgg cggtgcgggt
     1201 tcgggatctc caggcccatg cacgatttgc ccgggatggt ctcgaccacc cggatcgaca
     1261 cgaccgacag cgcgcgcgcc aggtccttca ccaggttcac gatctggctg cccttcacgc
     1321 cgaccgcggg ctcgatctcg tagcgcgtga tgaccggccc ggggtacgcg gcaagcacct
     1381 tcacggcgac gccgaagtcg gaaagcttct tctcgatcag gcgcgaagtg aattccagcg
     1441 tctcggcggc gatcgtctcg cccccgcccg ccggctcgtc gagaagcttg agtggcggaa
     1501 gaggcgtatc cggcaggtgc tcgaaaagcg gcgcctgccg ctctttctgc gcgcgctcgg
     1561 atttcctgat ctcggcgagc gccggctcta tgcgcaacgg cgggtgatcg tcttcccggc
     1621 gcttctcgat ctcgaccacg gcctcgcgct gcgcgcgggc gcgggtgccg agcttgcggt
     1681 cccggcgacg ctcccaggcg gcgcggccgc cgccatacgt tttctcgaga cccgtcccga
     1741 ccgcttcgaa aatcgtaagc cacgaaacgc cgacgaacag gctgaacccg atggcggcga
     1801 acgtgagcag cgcgagggtc gcgccggtaa agccaaacgc gccggatagc ggcccggcga
     1861 gcacttcgcc gattaccccg ccgggcgcga gcggcagctc ggccggcagg ctatgcagcc
     1921 gcagggactc gagcgcggcg ctcgaagcga tgagcagcgc aaatccgccg agcgacacgg
     1981 cgagacgcgt gccgtccagg cgacggaagc cccagaccac gatgtaggcg cacaaggcag
     2041 cccaccagta ggccgacacg ccgaagaggt aaagaagaag atccgccagc caggcgccga
     2101 acgcaccgcc ggcattgcgc gccaccgcgt cagtggcgct atgcgaccag ccgggatcgg
     2161 agcgctggaa ggtggcgaag atcagcagca ggtaaagcgc cgccgcgatc aggagcagcc
     2221 atcttgcttc gcggagcagg cccgccagcc gggcggggag cggagtgccg gttttgctag
     2281 tcgggcgcag gaggaggtcc cccgtgcccc gttactccgg gagcatgatg gtgtcgcgca
     2341 gaacgatgct cgagccgcgc atctcgatgt agccgccggt ctgcaggtcc ttcatcaccc
     2401 ggctcaccat ctcgcgcgag gcgccgatca tcttggcgat gtcctgcttg ggcaggcgct
     2461 tggtgacgac tttctcgccg tcaacgttct ccgccatgtc gagcagcagg cgagccacgc
     2521 ggccgtacac gtcgaggagc gcaaggctgc cgatcttgcg gtcggcctcg cgcagccgct
     2581 tgacgaggcc gcgcatcacg gcctccgtca tctgccagtt ctcggccatg gccttcttga
     2641 agtcgcgctt ggcgatcgag agcagctcgc acggctcgat cgtcaccacc gtggccgagc
     2701 gcggctcgtc gtcgatgagt cccatctcgc cgaagaactc gcccggcccg aggatcgcga
     2761 ggatcacttc cttgccctcg gagtcgctca tcatgacctt cagccggccc gaaagcacga
     2821 tgtagagcga atcggtcgga tcgcctccgg ccatgatggt ggtggagcga ggc
//
LOCUS       DASUNT010000633         2037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_256293, whole genome shotgun sequence.
ACCESSION   DASUNT010000633 DASUNT010000000
VERSION     DASUNT010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256293"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..>2037)
                     /locus_tag="VFX94_12735"
     CDS             complement(81..>2037)
                     /locus_tag="VFX94_12735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5769092.1"
                     /translation="GALAQIDRFANADAGLSSVGYDPFNNAIAKRRLQELRQAWGEYH
                     KLLRPIVGFAAGPYVETADRGTQLSAAGLRLNRELDQAARFGLSRHEDMAKLVSSLAE
                     AVEQETSQRAADLRTYLFATLAVSAVLLIAVIFYFGRNLSRQEQSSTRAKKETDDILR
                     TVNQGLFLLDKELKLGSERSEALERIFRRTDFDKLSFDGLLRGIVPEKTLETAMEYVR
                     LLWGERVNEKLIKTINPLVEVEVHIDAGNGQLDTQYLEFDFNRVKADGVMSRLLVTVN
                     DVTRRVHLARELLESQEKAQAQLDLLLHILHVDPRQLTGFLEESEVAMKMVNSILKEP
                     AREETAFRAKLDGIFRQIHGVKGEAAALGLKTVESRAHGFEESLAELRSRPALSGNDF
                     LPLVVKLDDLFGHLASVREMVGRLADLRVAMTSDDAPRFSEHDDTDIGPAGTLIQPAP
                     QILQRMAEGAEAAGTLESSLKALASKVASEQSKQVHIVCSGLDNVPKSYRRAVKDITI
                     QMVRNCVAHGIETPAERSNAAKDAAGTIVLKFESRADGYELVCQDDGRGLSTEKLKQT
                     AIKRGLLSEEQAAQLDERRALSLIFMPGFSTQDGVTKDAGRGVGMDIVRNLVQELGGK
                     VGVSTTVGRFARFRIWLPGATGASRAA"
BASE COUNT          291 a          679 c          694 g          373 t
ORIGIN      
        1 cgcatgatgt tcgagtcgtc gacgatcatc agcttcaggg gtgcaaccgc gttcatgctc
       61 cgatcctttg ttctttaact tcaggccgcg cgcgaggcgc ccgtggcgcc cggcagccaa
      121 atgcgaaagc gcgcgaagcg ccccaccgtg gtggaaactc cgaccttgcc gccgagttcc
      181 tggaccaggt tgcgtacgat gtccatgccg acgccgcggc cggcgtcctt ggtcacgccg
      241 tcctgcgtcg agaagcccgg catgaagatg agcgacagtg cgcgccgctc atcgagctgc
      301 gcggcctgct cctcgctcag caggccgcgc ttgatggcgg tctgcttgag tttctcggtc
      361 gagaggccgc ggccgtcgtc ctggcacacc agctcgtatc catccgcgcg gctctcgaac
      421 ttgagtacga tggtgccggc ggcgtccttg gctgcgttgc tgcgctcggc gggtgtctcg
      481 atgccgtgcg cgacgcaatt gcgcaccatc tggatcgtga tgtccttgac cgcgcgccgg
      541 tagctcttcg gcacgttgtc caggcccgag cagacgatgt gcacctgctt ggactgctcg
      601 ctcgccacct tcgaggcgag cgccttgagc gagctttcga gcgtgccggc cgcttcggcg
      661 ccttccgcca tgcgttgcag gatctgcggc gcgggctgga tcagggtgcc cgccgggccg
      721 atgtcggtat cgtcgtgctc gctgaagcgc ggcgcgtcgt ccgaggtcat cgccacgcgc
      781 aggtcggcga gacgccccac catctcgcgt accgaggcga ggtgcccgaa caggtcgtcg
      841 agcttcacca ccagcggcag gaagtcgttg ccggacaggg ccgggcggct gcgcagctcc
      901 gcgagcgatt cctcgaagcc gtgggcgcgc gactcgaccg tcttcaggcc cagcgcggcc
      961 gcttcgccct tcacgccgtg gatctggcgg aagatgccgt cgagcttggc gcggaaggcg
     1021 gtctcctcgc gggccggctc cttgagaatc gagttcacca tcttcatcgc gacttcgctc
     1081 tcttcgagga agccggtgag ctggcgcgga tcgacgtgca ggatgtgcag cagcaggtcg
     1141 agctgcgcct gcgccttctc ctgcgactcg agcagctcgc gcgcgaggtg cacgcggcgc
     1201 gtgacgtcgt tgaccgtcac gagcaggcgc gacatgacgc cgtccgcttt cacgcggttg
     1261 aagtcgaact ccaggtactg cgtgtcgagc tggccgttgc cggcgtcgat gtgcacctcg
     1321 acctcgacca gcgggttgat ggtcttgatg agcttttcgt tgacgcgctc gccccacagc
     1381 aggcgcacgt actccatcgc ggtctcgagg gtcttctccg gcacgatgcc gcgcagcagg
     1441 ccgtcgaagg agagcttgtc gaaatcggtg cggcggaaga tgcgctccag cgcctcggaa
     1501 cgctccgagc cgagcttcag ttccttgtcg agcaggaaca gaccctggtt cacggtgcgc
     1561 agaatgtcgt ccgtctcctt cttcgcgcgc gtggagctct gctcctggcg cgagaggttg
     1621 cggccgaagt agaagatcac cgcgatcagc agcaccgccg acacggcaag cgtcgcgaag
     1681 aggtaggtgc gaaggtcggc ggcgcgctgg ctggtctcct gctccaccgc ctcggcgagc
     1741 gagctcacca gcttcgccat gtcctcgtgc cgcgacaggc cgaagcgtgc ggcctggtcc
     1801 agctcacggt tcaggcgcag gcccgccgcg ctcagctgcg tgccgcggtc tgcggtctcg
     1861 acgtaggggc cggccgcgaa gccgacgatc ggcctgagca gcttgtggta ctcgccccag
     1921 gcctgtcgca gctcctgcag ccggcgcttg gcgatcgcgt tgttgaaggg gtcgtagccg
     1981 acgctcgaga gtccggcatc ggcgttcgcg aaacggtcga tctgcgcgag agcgccg
//
LOCUS       DASUNT010000634         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_519865, whole genome shotgun sequence.
ACCESSION   DASUNT010000634 DASUNT010000000
VERSION     DASUNT010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519865"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFX94_12740"
     CDS             <1..321
                     /locus_tag="VFX94_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HEX5769093.1"
                     /translation="RIHYAVNCASIGCPMLREEAYVAVRLDEQLEEQARRFLSDRTRN
                     RFRGGRLEVSKIFDWYKEDFEPREEYFARYAGVLGVPPGTSPRLVFLDYDWALNDSRS
                     SSPR"
     gene            282..953
                     /locus_tag="VFX94_12745"
     CDS             282..953
                     /locus_tag="VFX94_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012732785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX5769094.1"
                     /translation="MGAQRLSVVVPALNEASGIRAALAALVPLRAGGHEVIVVDGGSS
                     DGTPELARGLCDHVLSAPRGRALQMNAGAAAATGDALVFLHADTHLPQGADELIFQSL
                     RLSPWGRFDVEIDARHPLLRIVACTMNLRSRLTGIATGDQAIFVRREVFPGFPDIELM
                     EDVAFCKAMKRFGPPACRRERVVTSGRRWESRGVLRTLVLMWRLRLMYFLGARPERLA
                     RIYYR"
     gene            950..1531
                     /locus_tag="VFX94_12750"
     CDS             950..1531
                     /locus_tag="VFX94_12750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX5769095.1"
                     /translation="MIIVFAKAPVPGEAKTRLIPRLGPWRAARLHVRLTLHALSTARA
                     ARCGPVELHLTRRHSLFKEARLQRGRDLGERMQHAFCRSRKAILIGIDCPMLSARDLR
                     RAARWLRGGYDAVLAPAEDGGYALIGLRKPIPALFEGIDWGSERVYADTVKKLARYRW
                     RALPRVWDLDRPQDLDRLRSLRFPLASRQGARR"
     gene            complement(1502..>2041)
                     /locus_tag="VFX94_12755"
     CDS             complement(1502..>2041)
                     /locus_tag="VFX94_12755"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX5769096.1"
                     /translation="AGCGERPKLERLAPDAVVLAFGDSLTYGTGATEDESYPAQLEKL
                     IGRRVVRAGVPGEVTAQALVRLPAALDEHAPRLVLLCIGGNDFLRRLGNHQAEKNVRE
                     MVKLAQSRGVAVFLIGTPEPGFSVSPPSFYGGIAKEYRVPYEGAIVGEVLKDRTLKAD
                     PIHRTHAATASSPSALPRR"
BASE COUNT          300 a          698 c          689 g          354 t
ORIGIN      
        1 cgcatccact acgcggtgaa ctgcgcctcg atcggttgcc cgatgctgcg cgaggaagcc
       61 tacgtcgccg tgcgcctgga cgagcaactc gaggagcagg cccggcgctt cctgtccgac
      121 cgcacccgca accggttccg cggcggacgt ctggaggtgt cgaagatctt cgactggtac
      181 aaggaggact tcgagccgcg cgaggagtat ttcgctcgct acgccggtgt cctcggcgtg
      241 ccgccgggca cgagcccgcg tctcgtgttc ctggactacg attgggcgct caacgactct
      301 cggtcgtcgt ccccgcgctg aacgaggcgt ccggaatccg cgccgcgctc gcagcgcttg
      361 tgccactgcg cgctggcggc cacgaggtaa tcgtggttga tggtggcagc agcgatggaa
      421 cgcccgagct tgcgcggggg ctgtgcgacc atgtgctaag cgccccgcgc gggcgcgcgc
      481 tgcaaatgaa cgccggcgcc gccgcggcga ccggggatgc gcttgtcttt ctgcacgcgg
      541 acactcatct gccgcaaggc gccgacgagc tgatcttcca atccctgcgc ctttccccct
      601 ggggaagatt cgacgttgaa atcgacgctc gccatccgct gctcaggatc gtcgcctgca
      661 ccatgaacct gcgctcgcgc ctcaccggca tcgcgacggg cgaccaggcg atcttcgtgc
      721 gccgcgaagt ttttcccggc tttcccgaca tcgagctgat ggaagatgtg gcgttctgca
      781 aggcgatgaa gcgtttcgga ccgcccgcgt gccggcgcga gcgcgtggtc acctcgggcc
      841 gtcgctggga gtcgcgcggc gtgctgcgca ccctcgtcct catgtggcgc ctgcgcctca
      901 tgtacttcct cggcgcgcgt cccgagcgcc tcgcgcggat ctactaccgg tgatcatcgt
      961 gttcgccaag gctcctgtgc ccggggaggc gaagacgcgg ctcattccgc gcctgggccc
     1021 ctggcgggct gcgcggctgc acgtgcgcct cacgctgcac gcgctaagca ctgcgcgcgc
     1081 cgcaaggtgc ggtccggtag agctgcatct tacgcgcagg cattcgctat tcaaggaagc
     1141 gcggcttcag cgagggcgcg atcttggcga acgcatgcag cacgcatttt gcagatcgcg
     1201 gaaagcgatc ctcatcggca tcgattgccc gatgctcagt gcgcgggacc tgcggcgcgc
     1261 ggcgcgctgg ctgcgcggtg gctacgacgc ggtgcttgct cccgcggagg acggcggcta
     1321 tgcgctcatc ggactaagga agccaattcc cgcgctcttt gagggcatcg actggggaag
     1381 cgagcgcgtg tacgcggaca cggtgaagaa gctcgctcga tatcgctggc gcgcgctgcc
     1441 ccgcgtctgg gacctcgacc ggccgcagga tctggaccgg ctcagatcgc tccgctttcc
     1501 cttagcgtcg cggcaaggcg ctcggcgatg atgcggtagc cgcgtgcgtt cggtggatgg
     1561 gatcggcctt gagagttcgg tccttcagca cttcgcccac gatcgcgcct tcgtagggca
     1621 cgcggtactc cttggcgatg ccgccgtaaa aagagggcgg cgaaacgcta aagcccggct
     1681 ccggtgtgcc gatcagaaac accgcaacgc cgcggctttg cgcgagcttc accatctcgc
     1741 gcacgttttt ttccgcctgg tggttgccca gccgccgcag gaaatcgttg ccgccaatgc
     1801 acagcagcac gagccgcggc gcgtgctcgt cgagcgcagc gggcagccga acgagcgcct
     1861 gcgcggtcac ctcgcccggc acgccagcgc gtacgacgcg ccgcccgatg agcttctcga
     1921 gctgcgccgg atagctctcg tcctcggtgg cgccggtgcc atacgtgagg ctatcgccga
     1981 aggcgagcac taccgcgtcc ggcgcaagcc gctcgagctt ggggcgctcg ccgcagcccg
     2041 c
//
LOCUS       DASUNT010000635         4565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1044245, whole genome shotgun sequence.
ACCESSION   DASUNT010000635 DASUNT010000000
VERSION     DASUNT010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044245"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..100
                     /locus_tag="VFX94_12760"
     CDS             <1..100
                     /locus_tag="VFX94_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HEX5769097.1"
                     /translation="CVWGGSLSALRLEDRKLYQIACRQYQTPGGDA"
     gene            97..516
                     /locus_tag="VFX94_12765"
     CDS             97..516
                     /locus_tag="VFX94_12765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX5769098.1"
                     /translation="MSDCVFCRIVARQISATVVHEDEHTLAFMDIGQVNPGHVLVAVK
                     AHVENIFVLEVAQACAVAGTMVRVARGIGRAFAPHGLSVYQANGAAAGQTVLHYHVHL
                     VPRYAGDGMALTWPVKNPAREKLEEYAAKIRASLAPG"
     gene            complement(499..1440)
                     /locus_tag="VFX94_12770"
     CDS             complement(499..1440)
                     /locus_tag="VFX94_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX5769099.1"
                     /translation="MSILVTGSIAYDTIMVFPDRFRNHLLPDQLHILNVCFLTPQMRR
                     EFGGTAGNIAYNLKLLGEEPLVMASVGEDIAPYLERFQELGIATRLLARVPGEFTAQG
                     FITTDLDDNQITAFHPGAMNHSHKNHVTRELGARLAIIGPDGKQGMLQHARECAAAQV
                     PFVFDPGQGLPMFSREELGEFLQLAQYVAVNDYEGKLLEEKTGRGLEALAREVEALVC
                     TLGANGSFILAAGKRYEVPCVAADAVVDPTGCGDAYRAGLLYGIAQGWDWPSTGRLGA
                     VMGAIKIAQRGGQNHAPTREQIERRFRAAFGYSPWGE"
     gene            complement(1437..2189)
                     /locus_tag="VFX94_12775"
     CDS             complement(1437..2189)
                     /locus_tag="VFX94_12775"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HEX5769100.1"
                     /translation="MSLVTRCPACRTAFRVRREQLAAHSGSVRCGKCAEVFNGITGLV
                     EEGAEQSSIDPSPQPGLFDPSRRPLPAARPPGEQVALPRFMAGTERAPGRAWLWAGAA
                     VAAAALLSAQVAYHYRAEIAAFLPEARAPLLAACQQLGCAVALPRRPDVLSIEASDLQ
                     ADPRRQGVIVLHALIRNRSRLPQQYPALELTLTDEGGRPVLRRVLLPRDYVEPEQARD
                     GIAAGGEAALRVYLDSGGERVTSYRLYLFYPA"
     gene            complement(2186..2995)
                     /gene="prmA"
                     /locus_tag="VFX94_12780"
     CDS             complement(2186..2995)
                     /gene="prmA"
                     /locus_tag="VFX94_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX5769101.1"
                     /translation="MAWLALTLRVEGTAAEAFSDALLEAGAQSVSLEGADMQTLAAVL
                     ASDTDPEALVACAARMAGIPVPAYKTGTIAERDWVRASQAQFAPLQVGRRLWIGASWH
                     RAPAARIALRIDPGLAFGTGSHPSTKLALAYLEDEIRGGERILDYGCGSGILAIAAAK
                     LGAALVHAVDLDPEAVETARANALANGVRLTAALPDALPAGDYDVVVANILAPVLIEL
                     APMLAQRSRRIALSGILETQAAELAQAYAPWFDMTIMASDEGWALLAGARK"
     gene            complement(2995..4353)
                     /gene="accC"
                     /locus_tag="VFX94_12785"
     CDS             complement(2995..4353)
                     /gene="accC"
                     /locus_tag="VFX94_12785"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX5769102.1"
                     /translation="MFEKILIANRGEIALRVQRACRELGIKTVAVHSEADADAKYVML
                     ADESVCIGPAPSRDSYLNIPAIISAAEVTDAEAIHPGYGFLAENADFAEKVERSGFVF
                     IGPRPENIRLMGDKVSAKAAMAKAGIPTVPGSDGALPDDPKEIVRIARKVGYPVIIKA
                     AGGGGGRGMRVVHTEAALLNAVNMTRSEAQAAFANPMVYLERYLEQPRHIEIQVLADA
                     HKNAVHLGERDCSLQRRHQKVMEEAPAPELPAKLRDRIGERCVDACRRIGYRGAGTFE
                     FLYENGEFYFIEMNTRIQVEHPVTEMVTGVDLVQEQIYVAAGEKLRMRQKDVQVRGHA
                     IECRINAEDPYRFTPSPGRITSYHPPGGPGIRVDSHVYQGYTVPPNYDSMVGKVIAYG
                     ATREQALGRMRIALSEMVVEGIQTNIELHRELLNDTRVMRGGVSIHYLEHKLAQTEKK
                     RK"
     gene            complement(4357..>4565)
                     /gene="accB"
                     /locus_tag="VFX94_12790"
     CDS             complement(4357..>4565)
                     /gene="accB"
                     /locus_tag="VFX94_12790"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX5769103.1"
                     /translation="GTFYRAPSPGAPSFVEVGQSVSKGQTLCIIEAMKLLNEIESDVA
                     GTVKAILVENGQPVEYGQPLFLIE"
BASE COUNT          706 a         1540 c         1552 g          767 t
ORIGIN      
        1 ctgtgtgtgg ggcgggtcgc tcagcgctct gcggctcgag gatcgcaagc tctatcagat
       61 tgcatgccgg cagtatcaaa ccccaggagg agacgcatga gcgattgcgt attctgcagg
      121 atcgtggcgc ggcagatttc cgcgaccgtg gtgcacgagg acgagcacac gctcgccttc
      181 atggacatcg gccaggtgaa cccgggtcac gtgctcgtcg ccgtgaaggc gcacgtggaa
      241 aatatcttcg tcctggaggt cgcccaggcg tgcgcggtcg ccggcaccat ggtccgcgtc
      301 gcgcgaggta tcggccgtgc ttttgctccg cacgggttat cggtctatca ggcgaacggc
      361 gcggcggccg ggcagacggt gcttcactac cacgtgcacc tcgtgccgcg ctatgcgggc
      421 gacggcatgg cgcttacctg gccggtgaag aatccggcgc gcgaaaaact tgaggagtac
      481 gcggcgaaga tccgcgcgtc actcgcccca gggtgagtag ccgaacgcgg cgcgaaaccg
      541 gcgctcgatt tgctcgcgcg tcggagcatg gttctgcccg ccgcgctgcg cgatcttgat
      601 tgcgcccatc acggcgccta gcctgcccgt gctcggccag tcccagccct gcgcgatgcc
      661 gtaaaggagt ccggcgcggt aggcgtcgcc gcagccggtc ggatctacca ccgcatcggc
      721 tgccacgcat ggcacttcgt agcgcttgcc cgcggcgagg atgaacgaac cgttggcgcc
      781 gagcgtgcac acgagagcct cgacctcgcg cgcaagggcc tccaagccgc gtccggtctt
      841 ctcttccagg agcttgcctt cgtagtcgtt caccgcgacg tactgggcaa gctgcaggaa
      901 ctcgccgagc tcctcgcgcg agaacatcgg caggccctgc cccggatcga agacgaaagg
      961 cacctgcgct gcggcgcact cgcgggcatg ctgcagcatg ccttgcttgc catccggtcc
     1021 gatgatcgct agccgcgcac cgagctcgcg cgtgacatgg ttcttgtgcg aatgattcat
     1081 cgctccgggg tggaacgcgg tgatctggtt gtcgtcgagg tccgtcgtga tgaatccctg
     1141 cgcggtgaac tccccgggca cccgcgcaag cagccgcgtg gcaatgccga gctcctggaa
     1201 gcgctcgagg tagggcgcga tgtcctcccc gacgctcgcc atgacgagcg gctcttcgcc
     1261 aaggagcttc aggttgtagg cgatgttgcc cgcggtgccg ccgaactcgc ggcgcatctg
     1321 gggagtaaga aagcacacat tgaggatgtg cagctggtcg gggaggagat gattacggaa
     1381 gcggtcgggg aacaccatga tggtgtcgta agcgatcgag ccggtgacga ggatgctcat
     1441 gcggggtaga agaggtagag acgatagctc gtaacgcgct cgccgcccga gtcgaggtac
     1501 acgcgcagcg ccgcctcgcc gccggccgcg ataccgtccc gggcctgttc cggctcgaca
     1561 tagtcgcgag ggagcagcac ccgccgcagc accggccggc cgccctcgtc ggtcagcgtg
     1621 agctccagcg cggggtattg ttgcggcagg cgcgagcggt tgcggatcaa agcgtgcagc
     1681 acgatcacgc cctgccggcg cggatcggcc tgaagatcgg acgcctcgat gctcagcacg
     1741 tcggggcggc gtggcagcgc cacggcgcag ccgagctgct ggcaggcggc caggagcggc
     1801 gcgcgtgcct cgggcaggaa cgccgcgatc tcggcgcgat agtgatacgc cacctgcgcg
     1861 ctcagtaggg ccgccgcggc gacggccgcc ccagcccaga gccaagcgcg gccgggcgct
     1921 cgttcggtgc ccgccatgaa cctgggcagc gcaacttgct ccccgggtgg gcgcgccgct
     1981 ggcagcggcc ggcgcgatgg gtcgaagagc ccgggttgcg gcgaagggtc gatggaggac
     2041 tgctcggcac cctcctccac gagtccggtg atcccattga aaacctcagc gcacttgccg
     2101 cagcggacgc tgccggaatg cgccgcgagc tgctcccgcc tcacccggaa cgcggtgcgg
     2161 catgccgggc agcgagtgac gaggctcatt tgcgcgctcc ggcgagcagc gcccagccct
     2221 cgtcactggc catgatcgtc atgtcgaacc acggcgcgta cgcctgcgcg agctccgctg
     2281 cctgcgtctc caggatgccg gaaagcgcga tgcgtctgct ccgctgcgca agcatgggcg
     2341 cgagctcgat cagcactggg gcgaggatgt tcgccacgac gacatcgtaa tcgccggcgg
     2401 gtagcgcatc gggcagcgcc gcagtcagcc tgacgccatt ggctagcgcg ttggcgcgcg
     2461 cggtctccac ggcttcgggg tccaggtcga ccgcgtggac cagcgctgcg ccgagcttgg
     2521 cggcggcgat cgccaatata ccggagccac atccgtagtc gagaatacgc tcgccgcccc
     2581 ggatttcgtc ttcgagatat gcaagcgcga gcttcgtgct ggggtggctg ccggtgccga
     2641 atgcgagacc cgggtcgatg cgtagcgcaa tgcgcgcagc gggcgcacgg tgccagcttg
     2701 ccccgatcca gagccggcgg ccgacctgga gcggcgcaaa ctgtgcctgg ctcgccctca
     2761 cccaatcgcg ctcggcgatc gtgccggtct tgtacgcggg caccgggatg ccggccatgc
     2821 gagcggcgca cgccacgagc gcctccgggt cggtgtccga agcaagcacc gcggccagcg
     2881 tctgcatgtc cgcgccttcg agcgaaacag actgcgcccc agcttcgagc agtgcgtcgc
     2941 tgaacgcctc ggccgccgtg ccctcgacgc gcagcgtgag cgcgagccag gccatcactt
     3001 tctcttcttt tccgtttgcg cgagcttgtg ctcgaggtaa tggatcgaca ccccgccgcg
     3061 catcacccgc gtgtcgttga gcagctcgcg gtgcagctcg atgttggtct ggatgccctc
     3121 gaccaccatt tccgacagcg cgatgcgcat gcgcccgagc gcctgttccc gcgtggcgcc
     3181 gtatgcgatc accttgccca ccatggagtc gtagttcggc ggcaccgtgt atccctggta
     3241 cacgtgcgaa tcgacccgga ttcccgggcc gccgggcgga tggtacgagg tgatccttcc
     3301 gggcgagggc gtgaagcggt acgggtcctc ggcgttgatg cggcattcga tggcgtggcc
     3361 gcgcacctga acgtccttct ggcgcatgcg aagcttctcc ccggcggcca cgtagatctg
     3421 ctcctgcacc aggtccacgc cggtcaccat ctcggtcacc ggatgctcga cctggatgcg
     3481 cgtattcatc tcgatgaagt agaactcgcc attctcgtag aggaactcga acgttccggc
     3541 gccgcggtag ccgatgcggc ggcacgcatc cacgcagcgc tccccgatcc tgtcgcgcag
     3601 cttcgccggc agctcgggcg ccggcgcctc ttccatcacc ttctgatggc ggcgctgcag
     3661 cgagcagtcg cgctcaccga ggtgcaccgc attcttatgg gcatcggcca gtacctggat
     3721 ctcgatgtgc cgcggctgct cgagatagcg ctcgaggtag accatcgggt tggcgaacgc
     3781 ggcctgcgcc tcgctgcgcg tcatgttgac ggcgttgagc agcgccgcct cggtgtgcac
     3841 aacgcgcatc cctcgcccgc cgccgccgcc ggccgccttg atgatcaccg gatagcccac
     3901 cttgcgcgcg atgcgcacga tctctttcgg gtcatcgggc agcgcgccat cggagcccgg
     3961 gacggtcggg attccggcct tggccatcgc cgccttggcg ctcaccttgt cgcccatcag
     4021 gcgaatattt tcgggccgcg gcccgatgaa gacgaaaccc gagcgctcca ccttttcggc
     4081 aaagtcggcg ttctcggcga ggaagccgta gccgggatgg atcgcctcgg cgtcggtcac
     4141 ctcggccgcg ctgatgatcg cggggatgtt gaggtagctg tcgcgcgagg gcgccgggcc
     4201 gatgcacacg ctctcgtcgg cgagcatcac gtacttggcg tcggcgtcgg cctcggagtg
     4261 caccgccacg gtcttgatgc ccagctcgcg gcaggcgcgc tgcacgcgaa gggcgatctc
     4321 gccgcggttg gcgatgagga tcttctcgaa catgccctac tcgatgagga acagcggttg
     4381 gccgtattcc accggctggc cgttctccac caggatcgcc ttcaccgtgc cggccacgtc
     4441 gctctcgatc tcgttcagca gcttcatcgc ctcgatgatg cacagcgtct ggcccttgga
     4501 gaccgactgc ccgacctcga cgaaggaggg cgcccccggc gacggcgcgc ggtagaaggt
     4561 gccca
//
LOCUS       DASUNT010000636         3849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1132473, whole genome shotgun sequence.
ACCESSION   DASUNT010000636 DASUNT010000000
VERSION     DASUNT010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132473"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /gene="lpxK"
                     /locus_tag="VFX94_12795"
     CDS             <1..816
                     /gene="lpxK"
                     /locus_tag="VFX94_12795"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX5769104.1"
                     /translation="VGGSGKTPLVIWVAQFLKSRGRSPGIIARGYGVRLREPRAATIA
                     SEAAEVGDEPVILARRSGCPVWVGPDRVEVAAALRRQHPEVDVLVLDDGLQHYRMRRD
                     VEIVVVDARGFGNGFMLPAGPLREPRLRLRTVDAVVSHGARIAGYPMELQGEELHRMT
                     DARERRALKSFAGQKVHAAAGIGDPNRFFLHLGRAGLKVMPHPFPDHYAFPGAELEFG
                     DGLPVILTEKDAVKLRSRARPEWWVLPVSASLDAAFGAWLLGKLDECRRSKAA"
     gene            788..979
                     /locus_tag="VFX94_12800"
     CDS             788..979
                     /locus_tag="VFX94_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX5769105.1"
                     /translation="MSAVDPRLLEILVCPLCKGKLVYRKPAAELVCKADRLAYPVKDG
                     IPVMLEDEARKLPPEEEVV"
     gene            976..1701
                     /gene="kdsB"
                     /locus_tag="VFX94_12805"
     CDS             976..1701
                     /gene="kdsB"
                     /locus_tag="VFX94_12805"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX5769106.1"
                     /translation="MNFRVIIPARYASTRFPGKALAELAGKPMVVHVCERAAKSGATA
                     VHVATDDERIARAVEQAGFAAVMTSPRHASGTDRIAEAAKKLGFKAADIVVNVQGDEP
                     LISPRLIRDVGTLLRKRRDAAVATACHPIHDSAAAASPNVVKVVLDAKGYALCFSRSQ
                     IPYPREAGAAWLRHAGIYGYRVGFLKTFARLKPSPLEKAEALEQLRALWHGYRIAVAV
                     SKAEIPPGVDTPQDLEAVRRILA"
     gene            1698..2333
                     /gene="adk"
                     /locus_tag="VFX94_12810"
     CDS             1698..2333
                     /gene="adk"
                     /locus_tag="VFX94_12810"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX5769107.1"
                     /translation="MKLILLGPPGAGKGTQAKFICERFGIPQISTGDMLRAAIKAGTA
                     LGRQAKQVMDAGQLVSDDIIISLVRERLKQRDCARGYLFDGFPRTIPQAEAISREGVA
                     IDHILEIDVPDEDIITRMSGRRVHPASGRVYHVKFNPPKAQGRDDQTGEPLVQREDDR
                     EHTVRKRLEVYRAQTRPLIDYYQRHAAGKYRRISGRGSVEDVKQRALAALT"
     gene            2368..>3849
                     /locus_tag="VFX94_12815"
     CDS             2368..>3849
                     /locus_tag="VFX94_12815"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX5769108.1"
                     /translation="MNAGLIFALVCALLAIAYGVWQSASILRLPDGNARMREIAAAVR
                     EGAIAYLWRQYKTIALVGVVLFILIGFFIDWPSAWGFFIGAVLSGACGIIGMNISVRA
                     NVRTAEAARIGLNEALQVAFRGGAITGLLVVGLGLLGVAGYFALLYANALDKKDLAHI
                     IHPLVGLGFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNV
                     GDNVGDCAGMAADLFETYAVTIIATMLLGALMVKTPGALIYPLALGGFAIIASIVATW
                     FVRAKPGDRNVMPALYRGLWVAGGLALVAFWPITLYIMGDVVREVPFEIAGGKKLISV
                     WHIWGAAVVGLVLTGFLVWITEYYTGTQFKPVHHVAEASITGHATNIIAGLGVSMKST
                     AWPVVAVCVAIFVSDWLAGLYGIAIAATSMLSMAGIVVALDAYGPITDNAGGIAEMAG
                     LPDSVRAITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTH"
BASE COUNT          596 a         1312 c         1307 g          634 t
ORIGIN      
        1 gtcggcggca gcggcaagac gccgctcgtc atctgggtcg cgcaattcct gaagagccgg
       61 ggccgctcgc cgggtatcat cgcgcgcggg tacggcgtgc ggctgcgcga gccgcgcgcc
      121 gcgacgatcg cctcggaggc ggccgaagtc ggcgacgagc cggtgatcct tgctcgccgc
      181 agcggctgtc ccgtctgggt gggccccgat cgtgtggagg tcgccgccgc gctgcgcagg
      241 cagcatcccg aggtcgacgt gctcgtgctc gacgacgggc tgcagcacta ccgcatgcgc
      301 cgcgacgtcg agatcgtggt ggtggatgcg cgcgggttcg gcaacggctt catgctgccc
      361 gccggcccgc tgcgggagcc gcggcttcgc ctgcgtaccg tggacgcggt cgtctcgcac
      421 ggcgcgcgca ttgccggcta cccgatggag ctccagggcg aggagctgca tcgcatgacc
      481 gacgcgcgcg agcgccgggc gctcaagtcc ttcgccgggc agaaagtgca cgcggcggcg
      541 ggcatcggcg acccgaaccg ctttttcctg caccttggcc gggcggggct caaagtcatg
      601 ccgcatcctt tcccggacca ttacgccttt ccgggagcgg agctcgagtt cggcgacggc
      661 ctgccggtga tcctcaccga gaaggacgcg gtaaaattgc gcagccgcgc gcggcccgaa
      721 tggtgggtgc tgcccgtgag cgcaagcctc gatgccgctt ttggtgcgtg gctcctggga
      781 aaactcgatg agtgccgtcg atccaaggct gcttgagatc ctcgtctgcc ccttgtgcaa
      841 gggcaagctc gtctatcgca agcccgcggc cgagctcgtc tgcaaggccg atcgcctcgc
      901 ctacccggtc aaggacggca tcccggtgat gctcgaggac gaagcgcgca agctgccccc
      961 cgaagaagaa gtggtttgaa cttccgggtc atcatcccgg cgaggtacgc gtccacgcgc
     1021 ttcccgggaa aggcgctcgc cgagctcgcc ggcaagccga tggtggtgca tgtgtgcgag
     1081 cgggcggcga agagcggggc taccgcggtg cacgtggcca ccgacgacga gcgcatcgcc
     1141 cgggcggtcg agcaagccgg cttcgctgcc gtgatgacga gcccgcgcca cgcctcgggc
     1201 acggaccgca ttgcggaggc ggcgaagaag ctcggcttca aggctgcgga catcgtggtc
     1261 aacgtgcagg gcgacgagcc attgatttcg cctcgcctga tccgggacgt cggcacgctc
     1321 ttgaggaagc gccgggacgc ggccgtggcg acggcgtgcc atccgattca cgactcggcc
     1381 gcggccgcga gcccgaacgt ggtgaaggtc gtgctcgacg ccaagggcta tgcgctctgc
     1441 ttctcgcgct cgcagattcc ctatccgcgc gaggccgggg cggcctggct gcgccatgcc
     1501 ggcatctacg gctaccgcgt cggcttcctc aagaccttcg cccgcctcaa gccttccccg
     1561 ctcgaaaaag cggaagccct cgagcaattg cgagcgctct ggcacggcta tcgcattgcg
     1621 gtagcggtga gcaaggccga gattccgccg ggagtcgaca cgccgcagga cctggaggcg
     1681 gtgcgtagaa ttcttgcatg aagctcatcc tcctcgggcc acccggcgca ggcaagggga
     1741 cgcaagccaa gttcatctgc gagagattcg gcatcccgca gatctccacc ggcgacatgc
     1801 tgcgcgccgc gatcaaggcc ggcactgcgc tcggccggca ggcaaagcag gtcatggacg
     1861 cgggccagct cgtttccgac gacatcatca tcagcctcgt gcgcgagcgc ctgaagcagc
     1921 gcgactgcgc gcgcggctac ctgttcgacg gcttcccgcg cacgatcccg caggccgagg
     1981 cgatcagccg cgagggcgtg gcgattgacc acatactcga gatcgatgtt ccggacgaag
     2041 acatcattac ccgcatgagc ggccggcgcg tgcaccccgc ctcggggcgc gtctaccacg
     2101 tgaagttcaa ccccccgaag gcgcaaggac gggacgacca aaccggcgag cccctggtgc
     2161 agcgcgaaga cgatcgcgaa cacacggtgc gcaagcgcct cgaggtctac cgcgcgcaga
     2221 ctcggccgct catcgactac taccagcgcc atgccgccgg caaataccgc aggatctccg
     2281 ggcgcggcag cgtcgaggac gtgaagcagc gcgccctcgc tgcgctgacc tgataaaatt
     2341 ttcccccttt ccagggggat agatcctatg aacgcaggcc tcattttcgc gctcgtctgc
     2401 gcgctcctcg ccatcgcgta cggcgtctgg cagagcgcaa gcatcctgcg cctgcccgac
     2461 ggcaacgcgc gcatgcgcga gatagcggcg gccgtgcgcg agggcgcgat cgcgtatctc
     2521 tggcgtcagt acaagacgat cgccctggtc ggcgtggtgc tcttcatcct catcgggttc
     2581 ttcatcgact ggccctcggc gtggggcttc ttcatcggcg cggtgctctc cggtgcgtgc
     2641 ggcatcatcg gcatgaacat ctccgtgcgc gccaacgtgc gcaccgccga ggcggcgcgc
     2701 atcggcctga acgaggcgct gcaggtggct ttccgcggcg gcgcgattac cggcctgctg
     2761 gtcgtcggcc tcggcctgct cggcgtcgcg ggctacttcg ccctgcttta cgccaacgcg
     2821 ctggacaaga aagacctcgc tcacatcatc cacccgctcg tcgggctcgg ctttggcggc
     2881 tcgctcatct cgatctttgc gcgtctcggc ggcggcatct tcaccaaggg ggccgacgtc
     2941 ggcgccgatc tcgtcggcaa ggtcgaagcc gggattcccg aggacgaccc gcgcaacccg
     3001 gcggtcatcg ccgacaacgt cggagacaac gtcggcgact gcgccggcat ggcggcggac
     3061 ctcttcgaga cctacgcggt caccatcatc gccaccatgc tgctcggcgc gctgatggtg
     3121 aagacgccag gcgcgctcat ctatccgctc gcgctcggcg gctttgccat catcgcctcg
     3181 atcgtcgcga cctggttcgt gcgcgcgaag ccgggcgaca ggaacgtcat gccggcgctg
     3241 taccgcggcc tctgggtcgc gggcgggttg gcgctcgtcg cgttctggcc gatcacgctc
     3301 tacatcatgg gggacgtggt gcgagaagtt ccgttcgaga tcgccggggg caagaagctg
     3361 atcagcgtct ggcatatctg gggcgccgcg gtggtggggc tcgtcctcac gggcttcctg
     3421 gtgtggatca ccgagtacta caccggcacg cagttcaagc cggtgcacca tgtcgccgag
     3481 gccagcatta ccgggcatgc gaccaacatc attgccggct tgggcgtgtc gatgaagtcg
     3541 acggcgtggc cagtggtcgc ggtgtgcgtc gcgatcttcg tgtccgattg gctcgcaggg
     3601 ctctacggca tcgcgatcgc cgccacctcg atgctgtcga tggcgggcat cgtcgtcgcg
     3661 ctcgacgcgt acgggccgat caccgacaac gccggcggca tcgccgagat ggccggtctt
     3721 cccgactcgg tgcgcgccat taccgacccg ctggatgcgg tcggcaacac caccaaggcg
     3781 gttaccaagg gctacgcgat cggctccgcc ggcctcgccg cgctggtgct gttcgccgac
     3841 tacacgcat
//
LOCUS       DASUNT010000637         2317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1220805, whole genome shotgun sequence.
ACCESSION   DASUNT010000637 DASUNT010000000
VERSION     DASUNT010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220805"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..587)
                     /locus_tag="VFX94_12820"
     CDS             complement(54..587)
                     /locus_tag="VFX94_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769109.1"
                     /translation="MERAAFAAEEVKKKVVVNDRMQRGYVYFLTEPEGRNFDPEFRPQ
                     LTPSELLKIGVFGGKYMTDCRAEFPAAWFERAKLCAERHEPELNFFKVNASQPLSTWR
                     AKGWIHPDDPRGWFQWYCRYYMGRRAPDDARQIRRWKNIRRHLAQIEKHCARRDLSCR
                     PRQRQSLLHWAYDSREV"
     gene            complement(554..898)
                     /locus_tag="VFX94_12825"
     CDS             complement(554..898)
                     /locus_tag="VFX94_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769110.1"
                     /translation="MEKKLNLKSGMKLRVLGKPAPVALAGVETTASAKAGAVIVFART
                     LAEVDANAGPAVDAAKADRIAWIAYPKAGQLDTDINRDILWKHLLKKGVQAVRQVAID
                     DVWSALRLRPKK"
     gene            complement(901..1605)
                     /locus_tag="VFX94_12830"
     CDS             complement(901..1605)
                     /locus_tag="VFX94_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5769111.1"
                     /translation="MLAIRDLDLYYGDAQALDRVTLDVAASEIVAIVGANGAGKSSLI
                     RSIAGMEKPRSGKVLFQESDITGRESHEICNLGIGQVAEGRQIFPSLSVLENLQMGAM
                     LTRARAAAKRNLEDVFALFPRLAERRHQVAGTMSGGEQQMIAIGRCLMGAPELIMFDE
                     PSLGLAPVVVQEVLHCIRLLNERGLTIVLVEQNVAVSLRISSRAYVLENGRIVMSGTG
                     DKLLHDDRVRQAYLGL"
     gene            complement(1605..2315)
                     /locus_tag="VFX94_12835"
     CDS             complement(1605..2315)
                     /locus_tag="VFX94_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5769112.1"
                     /translation="MRNISKSFRGLRAVRDVSFEIPEGTISALIGPNGAGKTTIFNMV
                     AGVYAPNSGSILFQTRPIHGLRPDEVCAAGIGRTFQIVKPFAGLSVLDNVMVGAFLNE
                     RHAAAARRHAADILEKLGLGPRLHLPASALTLPDRKRLEVARALATRPKLLLLDEVMA
                     GLRPTECDQMVEVFRELNRTQGLTILLIEHVMRAVMALARHIGVLHHGELIARGAPEQ
                     VVRDPAVLECYLGEETEI"
BASE COUNT          372 a          754 c          777 g          414 t
ORIGIN      
        1 ggagttttaa cttacttcgc aaagcttcgg agttagggct ccgtcccgaa tcgctacacc
       61 tctcgggagt cgtaggccca gtgcaggagc gactggcgct ggcgcgggcg gcacgagagg
      121 tcgcgccgcg cgcagtgctt ctcgatctgc gcgaggtggc ggcggatatt cttccagcgg
      181 cggatctggc gcgcgtcgtc gggtgcgcgg cgccccatgt agtagcggca gtaccactgg
      241 aaccagccgc gggggtcgtc cgggtggatc cagcccttcg ctcgccacgt gctgaggggc
      301 tgcgatgcgt tgaccttgaa gaaattcagc tcgggttcgt ggcgctcggc gcagagcttc
      361 gcgcgctcga accacgcggc cgggaactcg gcgcggcagt cggtcatgta tttcccgccg
      421 aagacgccga tcttgagcag ctccgagggc gtgagctgcg gcctgaactc gggatcgaaa
      481 ttcctgccct cgggctcggt caggaagtac acgtagccgc gctgcatgcg atcgttgacg
      541 acgactttct tcttcacttc ttcggccgca aacgcagcgc gctccataca tcgtcgatgg
      601 cgacctggcg cacggcctgt acgcccttct tcagcaggtg cttccaaagg atgtcgcggt
      661 tgatatcggt gtcgagctgg cctgccttgg gataagcaat ccacgcgatg cggtccgcct
      721 tcgccgcgtc aaccgccggg ccggcgttgg cgtccacttc cgcgagagtt ctcgcgaaga
      781 cgatgacggc gccggccttg gccgacgcag tggtttcaac gccggcaaga gcgacaggcg
      841 cgggcttgcc gagcacgcga agcttcatgc cgctcttgag gttgagcttc ttctccattc
      901 tcacagtccg agatacgcct ggcgcacgcg gtcgtcgtgc agcagcttgt cgccggttcc
      961 gctcatgacg atacgcccgt tttcgagcac gtaggcgcgg ctcgagatcc tgagcgacac
     1021 ggcgacgttc tgctccacca gcacgatggt aaggccgcgc tcgttcagca gccgtatgca
     1081 atgcagcact tcctggacca caaccggcgc gagcccgagc gagggctcgt cgaacatgat
     1141 gagctcgggc gcgcccatca ggcagcggcc gatcgcgatc atctgctgct cgccgccgga
     1201 catggtgccg gcgacctggt gacgccgctc cgcaagtcgc gggaagagcg cgaatacgtc
     1261 ctccaggttg cgcttcgccg cggcgcgggc gcgcgtcagc atggcgccca tctgcaggtt
     1321 ctcgagcacc gagaggctgg ggaaaatctg gcggccctcc gccacctgcc cgatgccgag
     1381 gttgcagatc tcgtgcgact cgcgcccggt aatgtcgctt tcctgaaaca gcacttttcc
     1441 gctgcgtggc ttttccatgc cggcaatgct tcgaatgagc gaggacttgc cggcgccgtt
     1501 cgcgccgacg atcgcgacga tctcgctcgc ggccacgtcg agcgtcacgc ggtcgagcgc
     1561 ctgggcgtcg ccgtagtaga gatcgaggtc gcgaatcgcg agcatcagat ctcggtctcc
     1621 tccccgaggt agcattcgag caccgccggg tcacgcacca cctgctcggg cgcgccgcgc
     1681 gcgatgagct cgccgtggtg cagcacgccg atgtgccgcg cgagcgccat cacggcgcgc
     1741 atgacgtgct cgatgaggag gatggtgaga ccctgggtgc ggttgagctc gcggaatacc
     1801 tccaccatct ggtcgcattc ggtcgggcgc agcccggcca tgacctcgtc gaggagcagg
     1861 agcttcgggc gcgtggcgag cgcgcgcgcc acctcgaggc gcttgcgatc cggcagcgta
     1921 agagcggagg cgggcaggtg gagcctcggc ccgaggccga gcttctccag gatgtcggcc
     1981 gcgtgccggc gcgcggccgc ggcatgacgt tcgttcaaga aagcaccgac catcacgttg
     2041 tcgagcaccg acagcccggc gaaaggcttg acgatctgga acgtgcggcc gatgccggcg
     2101 gcgcacactt catcgggccg cagcccgtgg atcggccttg tctgaaacaa gatcgaaccg
     2161 gagttcgggg cgtatacgcc cgccaccatg ttgaagatcg tggtcttgcc cgcgccgttc
     2221 ggcccgatga gcgcgctgat cgtgccctcg ggaatttcga acgatacatc gcgcaccgcg
     2281 cgcaggccgc ggaacgattt cgagatgttc cgcactt
//
LOCUS       DASUNT010000638         4970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_869632, whole genome shotgun sequence.
ACCESSION   DASUNT010000638 DASUNT010000000
VERSION     DASUNT010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869632"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VFX94_12840"
     CDS             complement(<1..890)
                     /locus_tag="VFX94_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5769113.1"
                     /translation="MQQVVKTPQGAQKYEGRLALGHVLDWLVADGMAGAEAAGLLKKE
                     RRYYRGTTHPLVIVADQKWKIGNTLLTLDVLSEWLAKRVGMEYMHIDPLKIDFAAVTD
                     MMSSAYATRFRILPVAVTPKEAVIATAEPGVRDWEPELARIIKRDIIRVISNPLDIER
                     FQVEFYNLAKSMKGAKVSHGGAGGLSNFEQLVELGKTNKQLDANDQGVVQVVDWLWQY
                     AFDQRASDIHLEPRREQGVIRFRIDGVLHPVYQVPLTVMNAMIARIKLLGRMDVVEKR
                     RPLDGRNKTTRPDTADGPGG"
     gene            958..1395
                     /locus_tag="VFX94_12845"
     CDS             958..1395
                     /locus_tag="VFX94_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEX5769114.1"
                     /translation="MRAKLINEHEQRVFAVIFDTGEDPMKGLERFAAEHDLDASGFTA
                     IGAFSEAMLGYFDWEKKDYEPIPVREQTEVLALLGDITLDEAGGRKVHAHAVLGKRDG
                     SACGGHLLSAKVRPTLEVILTESPGYLQRRHDPASGLALIRLD"
     gene            1449..1988
                     /locus_tag="VFX94_12850"
     CDS             1449..1988
                     /locus_tag="VFX94_12850"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX5769115.1"
                     /translation="MATSVLGCIALALALLGPLHDASERSLAAHMVQHMLLIGVAAPL
                     LALSLPALTFSPAGAFALHGAAVWLAHAPALIAWMAASHAAHMAAHATLLGTALLFWS
                     TVLRRRGEAALWVLATLIHTGLLGALLTFAPRELYGAYSLADQQLAGLLMWVPGGLLY
                     LAAGLVFARRAIASHAVRP"
     gene            1972..2844
                     /gene="coxB"
                     /locus_tag="VFX94_12855"
     CDS             1972..2844
                     /gene="coxB"
                     /locus_tag="VFX94_12855"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX5769116.1"
                     /translation="MQSALNPAGPTAALIAELAWVLFAGGALIFAGVMALAAYAVFSD
                     KRSKLSPRALIVGGGIAFPVLVLSALLGYSLVRMSTLQAGGEPALRIEVIGEQWWWRV
                     RYPQFETANEIRIPVGKLVELELKSADVLHSFWVPALAGKLDMVPGRTNRMRVQADQP
                     GEYRGQCAEYCGGPHALMALFVVALPSEDFDNWAAKQREPASQENESFNKTCAGCHTV
                     RGTAAAGVLGPDLTHVGGRRTIGAALLPMNRGALAGWIASSQHLKPGNLMPSFTMLPG
                     DELSALAEYLEGLK"
     gene            2850..>4970
                     /gene="ctaD"
                     /locus_tag="VFX94_12860"
     CDS             2850..>4970
                     /gene="ctaD"
                     /locus_tag="VFX94_12860"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX5769117.1"
                     /translation="MNPIPRPAEELEELRRVWALPRGIRLLTEYNNNFIGGLYVATAF
                     VFFVLAGILALLMRTQLAVPGNDLMGPELYNQLFTMHGTMMMFLFAVPAVEAMGILLL
                     PNMLGARDMPFPRLSLYAYWAYAVGGLVFFTSLFFGLAPTGGWFMYPPLSSEAYSPGI
                     NEDFWLLGIGFIEISAIAGAIEIIVGILRTRAPGMTLARMPIFAWAMLIFALMVVFAF
                     PAVIVATMLLELERAFGWPFFDATRGGDALLWQHLFWFFGHPEVYIIFLPAAGMVSMI
                     IPAMTRAPLAGSRYVILALAATGFLSFGLWVHHMFATGIPALSVSFFQAASMAVAVPA
                     GIQVFAWIATFARGKAKLATPALFILGFFFTFVLGGLTGVMVAVVPFDWQAHDTYFVV
                     AHLHYVLIGGMVFPLFAGFYYWMPYVSRHALSEPVGRWVFWLTFGGFNIGFLPMHITG
                     LLGMPRRVYTYPAGLGWDALNLVSTIGAFMIALGVALFIVDFARRFRPSSEKNAGNVW
                     DAGTLEWLPNGNYSNRSIPVVESAYPLWDQPRLAELVDAGAYYLPGTATGKREALVTA
                     PLDGAPQYVLQMPPPGWAPFLAAIFTAAFFMLLTVKLVALAVICGAIAVGSIWRWAWQ
                     LDPGPVRTAVDIGGGVRVPVHCTGPASHSWWAMILLILVAAAIYGSALFSYLYLWLVS
                     PQVWPKPGEYPDLFAGLLTAALIGS"
BASE COUNT          695 a         1707 c         1602 g          966 t
ORIGIN      
        1 tcgccgccgg gaccatccgc ggtatccggc cgcgtggtct tgttgcgccc gtccaggggc
       61 cgccgcttct cgacgacatc catgcggccg agcagcttga tgcgcgcgat catcgcgttc
      121 atcaccgtca gcggcacctg gtagacgggg tgcagcacgc cgtcgatgcg gaagcggatc
      181 acgccctgct cgcgccgcgg ctccaggtgg atgtcgctcg cgcgctggtc gaaggcgtac
      241 tgccacagcc agtccaccac ctgcacgacg ccctggtcgt tggcgtcgag ctgcttgttg
      301 gtcttgccga gctcgaccag ctgctcgaaa ttggacaggc cgccggcgcc gccgtggctg
      361 accttggccc ccttcatcga cttggcgagg ttgtagaact cgacctggaa gcgctcgatg
      421 tcgagcgggt tcgagattac gcgaatgatg tcgcgcttga tgatgcgcgc gagctcgggc
      481 tcccagtcgc gcacgccggg ctcggcagtg gcgatcaccg cctctttcgg cgtgacggcg
      541 accggcagga tgcggaagcg cgtcgcgtac gccgaggaca tcatgtcggt gaccgcggcg
      601 aaatcgatct tgagcgggtc gatatgcatg tattccatgc caacgcgctt cgccagccac
      661 tcgctcagca cgtcgagcgt cagcagcgtg tttcctatct tccacttctg gtccgcgacg
      721 atcacaagcg gatgcgtcgt gccacggtag tagcgccgct ccttcttgag caggccagca
      781 gcctcggcgc ccgccattcc gtcggccacc agccagtcga gcacgtgacc cagcgcgagc
      841 cgtccttcgt acttttgcgc cccctgcggg gtcttaacta cttgttgcat aggcgaaaga
      901 ctataccgca aaggtcgaat tcgctcctgc ggcacgcttg ttgcccctgt aaaaaccatg
      961 cgagcgaagc tcatcaacga gcacgagcag cgcgtgttcg ccgtgatttt cgacacgggc
     1021 gaggacccga tgaagggcct cgagcgcttt gctgccgagc acgatctcga cgcatccggc
     1081 tttaccgcca tcggcgcgtt cagcgaggca atgctcggct acttcgactg ggagaagaag
     1141 gactacgagc cgatcccggt acgcgagcag accgaggtgc ttgccctcct gggggatatc
     1201 acgctcgacg aagcgggcgg caggaaagtg cacgcgcacg cggtgctcgg caagcgcgac
     1261 ggcagtgcgt gcggcggcca cttgctctca gcgaaggtgc gcccgacgct ggaagtgatc
     1321 ctgacggagt cgccgggcta cctgcagcgc cggcacgacc cggcgagcgg tcttgcgctg
     1381 atccgcctgg actagcgtcc cggcgtcagg aattgacggc gcgaatcctt gtccgaccac
     1441 gcatgcgcgt ggcgacatcg gttctaggat gcattgccct tgcgctcgcg ctcctcgggc
     1501 cgctgcacga cgcgtccgag cgcagcctcg ccgcgcacat ggtgcagcac atgctgctaa
     1561 taggagtcgc ggctccgcta ctcgcgctat cgctgcccgc actaacgttc tcgcccgccg
     1621 gcgcgttcgc gctgcacggc gccgcggtct ggctcgcgca tgcgcccgcc ctcatcgcct
     1681 ggatggcggc gagccacgcg gcccacatgg ccgcgcacgc gacgcttctc ggcaccgcgc
     1741 tccttttctg gtccaccgtc ctgcgccggc ggggcgaggc agcgctctgg gtgctcgcca
     1801 cgctgatcca caccggcctg ctcggcgccc ttctcacttt cgcgccgcgc gagctctacg
     1861 gcgcctattc gctcgccgac cagcagctcg ccggccttct catgtgggtg ccgggcggct
     1921 tgctgtatct cgccgccggc ttggtattcg cccgacgagc catcgcttct catgcagtcc
     1981 gcccttaacc ctgctggtcc gacggccgca ctcatcgccg agctcgcctg ggtgctcttc
     2041 gccggcggcg cgctgatctt cgccggcgtc atggcgctgg ccgcctacgc cgttttctcc
     2101 gacaagagat ccaagctctc tccccgggcg ctgatcgtcg ggggcggcat cgcgtttccg
     2161 gtcctcgtgc tcagcgcgct gcttgggtac agcctcgtga ggatgagcac gctgcaagct
     2221 ggcggcgagc ccgcgctgcg catagaggtg atcggcgagc aatggtggtg gcgggtgcgc
     2281 tacccgcagt tcgagacggc gaacgagatc cgcatcccgg tcggcaagct cgtagagctc
     2341 gagctcaagt cggccgacgt gctgcatagc ttctgggtgc cggcgcttgc tggcaagctc
     2401 gacatggtgc cgggtcgcac caaccggatg cgcgtgcagg ccgaccagcc cggcgagtac
     2461 cgcgggcagt gcgccgagta ctgcgggggc ccgcacgccc tcatggcgtt attcgttgtg
     2521 gctctcccgt cggaggactt tgacaattgg gctgcgaagc agcgcgaacc cgcttcccaa
     2581 gaaaacgagt cttttaacaa gacttgcgcc gggtgccaca ccgtacgcgg aacggccgca
     2641 gcaggcgtgc tcgggcctga cctgacgcat gtgggcggcc ggcgcacgat cggcgcggcg
     2701 ctcctgccga tgaaccgcgg cgcgctcgcg ggctggatcg cctcgagcca gcacctcaag
     2761 cccggcaacc tgatgccctc cttcaccatg ctgccgggcg acgagctgag cgcgctcgcc
     2821 gagtacctgg aggggctgaa gtagtgctgt tgaatcccat tccgcgtcct gcggaagagc
     2881 tcgaggagct aaggcgcgtc tgggcgctgc cgcgcgggat ccgccttctg acggaataca
     2941 acaacaactt catcggtggg ctgtacgtcg cgaccgcatt cgtgtttttc gtgctcgccg
     3001 gcatcctcgc gctcctcatg cgtacgcagc tcgcggtccc cggcaacgac ctcatgggcc
     3061 cggagctcta caaccaactc ttcaccatgc acggcacgat gatgatgttc ctcttcgccg
     3121 tgccggcggt ggaggcgatg gggatcctgc tgctgcccaa catgctgggt gcgcgcgaca
     3181 tgccgtttcc gcgcctctcg ctctacgcct actgggctta cgcggtaggc ggactcgtat
     3241 tcttcaccag cctcttcttc ggactggcgc cgaccggcgg ctggttcatg tacccgccgc
     3301 tcagctcgga agcgtactcg cccggaatca acgaagactt ctggctgctc ggcatcggtt
     3361 tcatcgagat ctcggcgatc gcgggcgcga tcgagatcat cgtcggcatc ctgcgcacgc
     3421 gcgcgcccgg catgacgctc gcgcgcatgc cgatcttcgc ctgggcgatg ctgatcttcg
     3481 ccctgatggt cgtgttcgcc ttcccggcgg tgatcgttgc gacgatgctg ctcgaactgg
     3541 agcgcgcgtt cggctggccc ttcttcgatg ccacgcgggg cggcgatgcg cttctgtggc
     3601 agcacctctt ctggttcttc ggccaccccg aggtctacat catcttcctg cccgccgccg
     3661 gcatggtctc gatgatcatc cccgcgatga cgcgcgcgcc gctcgcgggt agccgctacg
     3721 tgatcctcgc gcttgctgca accgggttcc tgtcgttcgg gctttgggtg caccatatgt
     3781 tcgccaccgg catcccggcg ctgtcggtgt cgttcttcca ggcggccagc atggcggtgg
     3841 cggtacccgc gggtatccag gtgttcgcct ggatcgcgac cttcgcgcgc ggcaaggcga
     3901 agctcgccac gccggcgctc ttcatcctgg gcttcttctt caccttcgtg ctcggcggcc
     3961 tgaccggcgt gatggtggcg gtggtgccgt tcgactggca ggcgcacgac acctatttcg
     4021 tggtcgcgca cctgcactac gtgctgatcg gcggcatggt attcccgctt ttcgccggct
     4081 tctattactg gatgccgtac gtgagccgcc atgcgctgtc cgagcctgtg ggacgctggg
     4141 tgttctggct caccttcggc ggcttcaaca tcggcttctt gccgatgcac atcaccgggc
     4201 ttctcggcat gccgaggcgc gtgtacacgt accccgccgg cctcggctgg gacgcgttga
     4261 acctcgtctc caccatcggc gccttcatga tcgctctcgg cgtcgcgctc ttcatcgtcg
     4321 acttcgcgcg ccgcttccgg ccctcgtcgg aaaagaacgc cggcaacgtg tgggatgcgg
     4381 gcacgctcga atggctgccg aacggcaact actcgaaccg cagcatcccg gtggtggaga
     4441 gcgcttatcc gctgtgggac cagccgcgcc tggcggagct ggtggatgcc ggggcttact
     4501 acctgcccgg cactgcgacg ggaaagcgcg aagcgctcgt caccgcgccg ctcgacggcg
     4561 cgccgcagta cgtgctgcag atgccgccgc ccggctgggc gcctttcctc gccgcgatct
     4621 ttaccgcggc gttcttcatg ctgctgacgg tgaagctcgt cgcgctcgcc gttatctgcg
     4681 gcgcgatcgc ggtcggctcc atatggcgct gggcgtggca gctcgacccg gggcccgtgc
     4741 gaacggcagt cgacatcggc ggcggcgtgc gcgtaccggt gcactgcacc gggccggcgt
     4801 cgcattcgtg gtgggcgatg atcctgctga tactcgttgc cgctgccatc tatggcagcg
     4861 cgctcttttc gtatctctac ctctggctcg tctcgccgca ggtgtggccg aagccgggcg
     4921 aatatccgga ccttttcgcc ggccttctta cggccgcgct gatcggctct
//
LOCUS       DASUNT010000639         3144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1132963, whole genome shotgun sequence.
ACCESSION   DASUNT010000639 DASUNT010000000
VERSION     DASUNT010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132963"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VFX94_12865"
     CDS             <1..487
                     /locus_tag="VFX94_12865"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5769118.1"
                     /translation="NRRTMLEPGQPAPSFALPDADMQEVSLSEFKGKKNVVLYFYPKD
                     GTPGCTLVTTDFSDHQDQFGKHDCVVLGVSRDDCITHADFRDRNGVTISLLSDPEGEV
                     CRKYGVIQEKQLTEGVRRECLVRSTFVIDKKGVIRHAAYNVNPRGHAAEVFDLVRRLR
                     S"
     gene            504..950
                     /locus_tag="VFX94_12870"
     CDS             504..950
                     /locus_tag="VFX94_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5769119.1"
                     /translation="MVVAKDTVVRLRVQMHDAQGVELQSASELTYLHGGYGELLETLE
                     RALEGKSPGDSVRLQLEPEQAFGEYDAALLRVEPAKRYGEGIEVGMEIEEDSRLYTVT
                     DVAAGKVVLDGNHPLAGMALRFFCQVLSVRRAQPEEIRGGVSSPRA"
     gene            947..1288
                     /locus_tag="VFX94_12875"
     CDS             947..1288
                     /locus_tag="VFX94_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769120.1"
                     /translation="MKRLLLVCALILAAPARALSPAAQEFMAISKELEPVQCEKRRLR
                     REIAFAEAERRGEDLRVLRKKFAALDADPQTAKLEKRLAELEPRVKASRDPEDLAAIS
                     LQQRQAFYRCD"
     gene            1297..2148
                     /locus_tag="VFX94_12880"
     CDS             1297..2148
                     /locus_tag="VFX94_12880"
                     /inference="COORDINATES: protein motif:HMM:NF020448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HEX5769121.1"
                     /translation="MLHITNGDCAAEVLSGAVGGTILPWRDVLHEGPVRAGLGLEELS
                     RERAHFIADSGWGDRSEVARSFAERDAAFRRAGRHDEVVLWFEHDLYDQLQLIQVLDG
                     LAELRGPPVSLVCEAEYLGTMAAERAAELFVLRNPVTRRHFNEAQAAWAAFRSPDPGD
                     IENVKSAALPFLAAALRRHLQEFPWTSDGLSLLERRVLEALRFGALSFRSLFAAVKED
                     PQFLGDAVLVWHLERLTREGLIEREQDRWKPAREARVRREPRWLGGYEVKDERVRWDP
                     GSGRLVA"
     gene            complement(2128..>3144)
                     /locus_tag="VFX94_12885"
     CDS             complement(2128..>3144)
                     /locus_tag="VFX94_12885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX5769122.1"
                     /translation="GLHSDTGRVLGQAVRATHTVTFIALKPGLLTLDGPDYCGLVHVA
                     DLGLDAREIVAPAAWVADPAAFKRLLKPRPRNFHKGMAGSLGILGGAAGMTGAALLAG
                     RAALKLGAGRVYVGLLSDEVPVDLASPELMLRHPDDALGQDLDAIVAGPGIGQGERAE
                     TLVGAALASDLPCVLDADALNLIAESEDLRHACARRSADTILTPHPAEAARLLALKTA
                     DVQADRVKAARILAANLNAHVVLKGAGSILVARDGHWFINTSGNAGMASAGMGDALSG
                     ILGALLAQRHSGESALVLGVHLHGSAADELVAGGAGPVGLTAGEIADAARRRWNAWLG
                     YKTS"
BASE COUNT          474 a         1052 c         1124 g          494 t
ORIGIN      
        1 gaatcgccgc acgatgctcg aacctggtca acccgctccg tcgttcgcgc tgcccgatgc
       61 cgacatgcag gaagtcagcc tgtcggagtt caagggcaag aagaacgtgg tgctctactt
      121 ctatccgaag gacggaacgc cgggctgcac gctggtcacc acggatttct cggatcacca
      181 ggaccagttc ggcaagcacg actgcgtggt gctcggcgtc tcgcgcgatg actgcattac
      241 gcacgccgat ttccgcgacc gtaacggcgt cacaatctcg ctgctctccg atccggaggg
      301 cgaggtgtgc cgcaagtacg gcgtaatcca ggaaaagcag ctcacggagg gcgtgcgccg
      361 cgagtgcctg gtgcgctcta cgttcgtcat tgataagaag ggcgtcatcc ggcacgccgc
      421 ctataacgtg aacccgcgcg gccacgccgc cgaagtgttc gatctggtga ggcggctgcg
      481 ctcctagggc cgcggttccg gtcgtggtgg tcgccaaaga cacggtcgtc aggctgcgcg
      541 tgcagatgca cgacgcgcag ggggtcgagc tgcagtcggc gtccgagctg acgtacctgc
      601 acggcggcta cggcgagctg ctggagacgc tcgagcgggc gctcgaaggc aagtcgccgg
      661 gcgattcggt gcggctgcag ctggagcccg aacaggcgtt cggggagtac gacgcggctt
      721 tgctgcgcgt cgagcccgcc aagcgctatg gcgagggcat cgaggtcggc atggagatcg
      781 aggaggattc gcgcctctac acggtgaccg acgtcgccgc cggcaaggtg gtgctcgacg
      841 gcaaccatcc gctcgccgga atggcgctgc gattcttctg ccaggtgctg agcgtgagga
      901 gggcgcagcc ggaggaaatc cgcggcgggg tatcatcgcc gcgcgcatga agcggctcct
      961 tctcgtctgc gcgctgatcc ttgccgcgcc ggcgcgcgcg ctgagcccgg ccgcgcagga
     1021 attcatggcg atctcgaaag agctggaacc ggtgcaatgc gagaagcgcc gccttcgccg
     1081 cgagatcgcg ttcgcggagg ctgagcggcg cggcgaggac ctgcgcgtcc tgcgcaagaa
     1141 attcgccgcg ctcgacgccg atccgcagac cgccaagctc gagaagcggc tcgccgagct
     1201 cgagccgcgc gtcaaggcga gccgcgaccc cgaggacctc gccgcgatca gcctgcagca
     1261 gcgccaggcg ttctatcgct gcgattgagt caggcgttgc tccacatcac gaacggcgat
     1321 tgcgccgccg aggtattgtc cggagcggtt ggcggtacga tcctgccctg gcgcgacgtg
     1381 ctgcacgagg gcccggtgcg ggcagggctc gggctcgaag aactgtcgcg cgagcgcgcc
     1441 cacttcatcg ccgacagtgg atggggcgat cgctccgagg tcgcgcgctc tttcgccgag
     1501 cgcgatgccg ctttccgtcg cgccgggcgg cacgacgagg tcgtgctgtg gttcgagcac
     1561 gacctgtacg accagctgca gctcatccag gtcctcgacg ggctcgccga gctgcgcggg
     1621 ccgccggtct cgctcgtgtg cgaagccgaa tacctcggca ccatggcggc cgagcgggcc
     1681 gccgagcttt tcgtgcttcg caatccggtg acgcggcgcc acttcaacga ggcgcaagcg
     1741 gcctgggcgg cgttccgttc tccggatccc ggggacatcg agaacgtgaa gtccgcggca
     1801 ttgccattcc tcgcagcagc cctgcgccgg catctgcagg agtttccgtg gacgagcgac
     1861 ggtctctcgc tcctcgagcg tcgggtgctg gaagcgctgc gcttcggcgc gctttcgttc
     1921 cggagcctgt tcgctgcggt gaaggaggat ccgcagttcc tgggcgacgc cgtgctcgtc
     1981 tggcatctcg agcgactcac gcgcgaggga ttgatcgagc gcgagcaaga ccgctggaag
     2041 ccggcgcggg aggcacgcgt gcggcgagag ccgcgttggc tcggcggcta cgaggtgaag
     2101 gacgaacggg tgcgctggga tcccgggtca ggacgtcttg tagcctagcc acgcgttcca
     2161 gcgccgccgc gccgcgtcgg cgatctcccc ggcggtgagc ccgaccgggc cggcgccgcc
     2221 tgcgacaagc tcgtcggcgg cggagccatg caggtgcacc ccgagcacaa gcgcgctctc
     2281 gccggaatgg cgctgcgcga gcagcgcgcc gaggatgccg gacaatgcgt cgcccatgcc
     2341 ggcgctcgcc atgccggcgt tgccgctcgt gttgatgaac cagtgcccgt cgcgcgccac
     2401 gaggatgctg cccgcgccct tcagcacgac atgcgcattc aggttcgcgg cgaggatcct
     2461 ggccgccttt acgcggtcgg cctgcacgtc ggcggtcttc aaggcgagca gccgcgccgc
     2521 ttccgcggga tgcggcgtca gtatcgtgtc cgcgctgcgg cgcgcacacg cgtggcgcag
     2581 gtcttcgctt tccgcgatga ggttcagcgc gtctgcgtcc agcacgcagg gcagatcgct
     2641 cgcgagcgcg gcgccgacca gcgtctcggc gcgttcgccc tgcccgatgc cggggccggc
     2701 gacgatggcg tcgaggtcct gcccgagcgc gtcgtccgga tgtcgcagca tcagctcggg
     2761 gctggccaaa tccactggca cttcgtcgct cagaagcccg acgtagaccc gtcccgcgcc
     2821 gagcttcagt gcggcgcggc cggcaagcag cgccgcgccc gtcatgccgg ccgcgccgcc
     2881 gaggatgccg agcgagcctg ccattccctt gtggaagttg cgcggccgag gcttgagcag
     2941 cctcttgaac gccgccggat cggctaccca ggcggcaggc gccacgatct cgcgcgcatc
     3001 gagcccgagg tccgccacgt ggaccaggcc gcaatagtcc ggtccgtcga gggtcagcag
     3061 ccccggcttg agggcgatga acgtgacggt gtgcgtcgcg cgtacggctt ggccgagcac
     3121 gcgcccggtg tcggagtgaa ggcc
//
LOCUS       DASUNT010000640         3539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1044928, whole genome shotgun sequence.
ACCESSION   DASUNT010000640 DASUNT010000000
VERSION     DASUNT010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044928"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /gene="ugpE"
                     /locus_tag="VFX94_12890"
     CDS             <1..701
                     /gene="ugpE"
                     /locus_tag="VFX94_12890"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HEX5769123.1"
                     /translation="GEHFWENYTQVLTAGSARGAGAPVGQMMLNSLVMALAIAFGKIA
                     ISIIASFAIVYFRFPLRNFFFWMIFVTLMLPVEVRIIPTFKVVSDLGMINSYVGLTLP
                     LIASATATFLFRQFFLTIPDELAEAARMDGAGPMRFFWDVVVPLSKTSVAALFVIQFI
                     YGWNQYLWPLLITTDESMYTTVIGIKRMVTGGDALTEWHLVLATAMLALLPPAVVVVL
                     MQKWFVRGLVETEK"
     gene            706..1701
                     /locus_tag="VFX94_12895"
     CDS             706..1701
                     /locus_tag="VFX94_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HEX5769124.1"
                     /translation="MAQVSFRDVRKSYAGLEVIHGVSVEVGDGEFVVIVGPSGCGKST
                     LLRMVAGLEQITSGEVAIGGRVVNNLEPKDRDIAMVFQNYALYPHMSVYDNMSYGLRI
                     RGFPKTDLDARVRKAAEILELSAFLQRKPRQLSGGQRQRVAMGRAIVREPAVFLFDEP
                     LSNLDAKLRVQMRAELQALHRRLGTTSLYVTHDQVEAMTLAHRMIVMNAGRAEQIGAP
                     LEVYAKPATTFVAAFIGSPPMNLIPQERDGRKMLFGIRPEHLEPCAESEAKLVADIDL
                     IEPLGADTLVYGHLGSGTRIAARLHSSVDARAGRLALRYRPEHAHYFDAQTGARL"
     gene            1702..2871
                     /locus_tag="VFX94_12900"
     CDS             1702..2871
                     /locus_tag="VFX94_12900"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEX5769125.1"
                     /translation="MLKPTPFHERTAALCASFAWRRWAGYVVASSYELSHEREYHAIR
                     GSAALFDVSPLYKYRIRGNDAARLLDLVVTRTIHDLNVGQVAYTPWCDAAGKVLDDGT
                     VARLGENEFRLTSAEPNLRWLEDNAVGLQVEIEDVSESLAALSLQGPASRAILGDIDL
                     RYFRLARRHLGGIPVAISRTGYTGDLGYEIWVSPRDAIALWDYLIEAGTPYGIVPAGM
                     LALDVARIEAGLVLIDVDYVPARKALIDSQTSSPYELDLAWTVNLGKDRFVGREALAR
                     EASRGAQWQFVGLEIDWTSLERLYAEVGLATRLPAAAWRTSVPVYAGNQQAGYATSGG
                     WSPLLKKYIALAHLESRHAAPGTQLDIEMTVEHRRKRAAVRVVKKPFFDPERKRA"
     gene            2864..>3539
                     /locus_tag="VFX94_12905"
     CDS             2864..>3539
                     /locus_tag="VFX94_12905"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5769126.1"
                     /translation="MPSYDAIVIGAGHNGLVCAAYLARAGRKVLVLERRERIGGAALT
                     EEVFPGFRFSVFSYVVSLLRPEIIRELELPRHGLHILPLESTLTPLAGGNYLAQWNDH
                     DQNRRELARHSLRDAEAYDEFGLMLHQMARAVKPLLALAPPDPHSLSFGEVRSLARLG
                     RYFRDLGRPHFNRLMKLLTMSSADFLDEWFETEALKATKSASGIIGTLLGPRSPGTAY
                     VLLHHYM"
BASE COUNT          563 a         1187 c         1158 g          631 t
ORIGIN      
        1 cgggcgagca cttctgggaa aactacaccc aggtgctgac cgccggctcc gcgcgcggcg
       61 ccggcgcgcc cgtcgggcag atgatgctga acagcctggt gatggcgctc gccatcgcgt
      121 tcggcaagat cgcgatctcg atcatcgcct cgttcgccat cgtctacttc cgctttccgc
      181 tgcgcaattt cttcttctgg atgatcttcg tcaccctcat gctgccggtg gaggtgcgca
      241 tcatcccgac cttcaaggtg gtgtcggatc tgggcatgat caactcctac gtcggcctga
      301 cgcttccgct catcgcctcg gcaaccgcga cgttcctctt ccgccagttc ttcctcacga
      361 tcccggacga gcttgccgag gcggcgcgca tggacggcgc ggggccgatg cgctttttct
      421 gggacgtcgt cgtgccgctc tccaagacga gcgtggccgc gctcttcgtg atccagttca
      481 tctatggctg gaaccagtac ctttggccgc ttctcatcac gaccgatgaa tcgatgtaca
      541 cgaccgtgat cggcatcaag cgcatggtca ccggcggcga cgcgcttacc gagtggcacc
      601 tggtgctcgc gaccgcgatg ctcgcgctcc ttccgccggc ggtggtcgtg gtgctcatgc
      661 agaagtggtt cgtccgcggc ctggtcgaga cggagaaata gcgcgatggc ccaggtctcg
      721 ttccgcgacg tccggaagtc ctacgccggc ctggaggtga tccacggggt atcggtggag
      781 gtgggggacg gcgagttcgt cgtcatcgtg ggcccgtcgg ggtgcggcaa gtcgacgctg
      841 cttcgcatgg tggcgggcct ggagcagatc acctcagggg aggtcgccat cggcggccgg
      901 gtggtcaaca acctcgagcc caaagaccgc gacatcgcga tggtgttcca gaactatgcg
      961 ctctatcccc acatgagcgt gtacgacaac atgtcgtatg gcttgcggat tcgtggcttt
     1021 ccgaaaacgg acctggatgc aagggtccgc aaggccgcgg aaatactcga gctgagcgcc
     1081 ttcctccaga gaaaaccgcg ccagctttcc ggcgggcagc ggcaaagggt cgccatgggg
     1141 cgtgccatcg tgcgcgagcc ggcagtgttc ctgttcgacg agccgctctc caacctcgac
     1201 gcgaagctgc gcgtgcagat gcgcgcggag ctccaggcgc tgcaccgcag gctcggcacg
     1261 accagcctct acgtcactca cgaccaggtc gaagcgatga ccctcgcgca ccgcatgatc
     1321 gtgatgaacg ctggtcgggc ggagcagatc ggggcgccac tcgaggtgta cgcaaagccc
     1381 gccacgacct tcgtcgccgc tttcatcggc tccccgccca tgaacctcat tccgcaggag
     1441 cgagacggcc gcaaaatgct ctttggcatc cggcccgagc acctcgagcc gtgcgccgag
     1501 tccgaggcga agctcgtcgc ggatatcgac ctcatcgagc cgctgggcgc cgataccttg
     1561 gtgtacggtc atctcggcag cggaacgaga attgcggcgc gccttcattc ttcggtcgac
     1621 gcgcgcgcag gcaggctcgc cttgcgttat cgtcccgagc atgcgcatta cttcgacgcg
     1681 cagaccggcg cccgcctcta gttgctgaaa cccacccctt tccacgagcg cacggcggcg
     1741 ctctgcgcaa gcttcgcctg gcgccggtgg gccgggtatg tagtggcaag ctcctacgag
     1801 ctgtcgcacg agcgggaata ccatgccatc cgcggctcgg cggcgctctt cgatgtgtcg
     1861 ccgctttaca agtaccgcat acgcggcaac gacgcggcgc ggctcctcga tctcgtggtt
     1921 acaagaacca ttcatgacct gaacgtcggg caggtggcgt acacgccgtg gtgcgacgcc
     1981 gccggcaagg tgctcgacga cggcaccgtc gcccggcttg gggaaaacga gtttcgcttg
     2041 acttcggccg agcccaacct gcgctggctc gaggacaacg cggtggggct ccaggtggag
     2101 atcgaggacg tgtcggagtc gcttgccgca ctgtcgctcc agggcccggc ttcgcgcgcc
     2161 atcctgggcg acatcgatct gcggtacttt cgtctcgcgc gcaggcacct tggcggcatc
     2221 ccggtcgcga tatcgcgcac cggctacacc ggggacctcg gctacgagat ctgggtctcg
     2281 ccgagggacg cgatcgcgct gtgggattac ctgatcgagg cgggcacgcc ttacggcatc
     2341 gtgccggcgg gaatgctcgc gctcgacgtg gcgcgcatcg aggcgggcct ggtgctgatc
     2401 gatgtggatt atgtgccggc gcgcaaggca ctgatcgaca gccagacctc gagcccctac
     2461 gagctcgatc tcgcctggac agtgaatctc gggaaggacc gctttgtcgg ccgcgaggcg
     2521 ctcgcgcggg aggcgagccg cggcgcgcag tggcagttcg tcggtctcga gatcgactgg
     2581 acctcgctcg agcgcctgta cgccgaggtc ggcctcgcca cgcgccttcc ggcggccgcc
     2641 tggcgcacga gcgtacccgt ctacgccggt aaccagcagg ccggctacgc gacgagcggc
     2701 gggtggtcgc cgctcctgaa gaagtacatc gcgctcgcgc atctcgagtc gcgccacgcg
     2761 gcgccgggca cgcagctcga catcgagatg actgttgagc accggcgcaa gcgcgcggcg
     2821 gtgcgcgtgg taaagaagcc gtttttcgat cccgagcgca agcgtgccta gctacgacgc
     2881 aatcgtcatc ggggccggcc ataacggcct ggtatgcgcc gcatacctgg cccgcgcggg
     2941 acgcaaggtt ctggtgctcg agcggcgcga gcgcatcggc ggcgctgcgc tgaccgagga
     3001 ggtgttcccc ggcttccgct tttcggtgtt ctcgtacgtg gtgagcctgc tgcgtccgga
     3061 gatcatccgc gaactcgagc tgccgcgcca cgggctccac atcctgcccc tcgagagcac
     3121 gctcacgccg ctggcgggcg gaaactatct cgcgcagtgg aacgaccacg accagaaccg
     3181 gcgcgagctc gcgcgccact cgctgcgcga cgccgaggcc tatgacgagt tcgggctgat
     3241 gctccaccag atggcgcgcg cggtgaagcc gctgctcgcg ttggcgccgc ccgacccgca
     3301 ctcgctctcg ttcggggaag tgagaagcct cgcgcgcctc ggccgctatt tccgcgatct
     3361 cggcaggccg cacttcaacc gtctgatgaa gctcctcacc atgagctcgg cggactttct
     3421 cgacgaatgg ttcgagaccg aggcgctcaa ggccaccaaa tcggcgagcg gcatcatcgg
     3481 cacgctgctt ggcccgcgtt cgcccggcac cgcctacgtt cttctgcacc actacatgg
//
LOCUS       DASUNT010000641         2805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_346004, whole genome shotgun sequence.
ACCESSION   DASUNT010000641 DASUNT010000000
VERSION     DASUNT010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346004"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VFX94_12910"
     CDS             complement(<1..501)
                     /locus_tag="VFX94_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEX5769127.1"
                     /translation="MKRVDHAEASSVFLKSTTHAEFHDKRLWDLRQKRDREAHAIPEW
                     EELRNLASAIKEHTLSHLADYLEQFERNAIANGMRVHWARDAAEHNQIVFDLLEKNRV
                     RVLVKSKSMLTDECEMRPFLETRSIEVIETDLGERIQQLDDEPPSHIVVPAVHKLRAD
                     VAEVFGK"
     gene            complement(498..1211)
                     /locus_tag="VFX94_12915"
     CDS             complement(498..1211)
                     /locus_tag="VFX94_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX5769128.1"
                     /translation="MKIGLFIPCYVDTFFPEIGVATLELLERLGVDVGYPLDQTCCGQ
                     PMANSGCHDDAAPAERLFRKNFARFDYVVAPSGSCTHHVRERAKLHPNALELTEFLHD
                     VLQVREFPWAKFPHKVGLHVSCAGLRGLGLASTSEIAGRAFSKPLALLSGVKGVQIVT
                     PARPDECCGFGGTFSVTEEPVSARMGYDKVNDHLAAGAEYIVTPDPSCMMHQKGCAER
                     LGLALKFIHIAQVLNGARA"
     gene            complement(1211..2170)
                     /locus_tag="VFX94_12920"
     CDS             complement(1211..2170)
                     /locus_tag="VFX94_12920"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5769129.1"
                     /translation="MKRFTVAGALALFASLSFAQAYPARPVTIVVPFAAGGPTDIIAR
                     ISGEYFSKALGQQFVVENVAGAGGTTGITRAAQAKPDGYTIMMGHMGTHGAAPALYKN
                     LKYDPAKDFTPIGLVAGTPILIVARKDFPAANLKEFIAKLKDPGAKVKQAHAGVGSVS
                     FTTCTLLSSQVGAKSIVAAYRGTGPALNDLVGGQVDYMCDQIVNLVEQVKGGSIKAYA
                     IATAERSPALPDVPTTKEAGLPDYQVSAWNALFAPKGLPREIHTRLNETMVKALDDEA
                     TRKRLLDLGSVIPDKAGRSPEALQKLVESEVKRWDPILKAYAQ"
     gene            complement(2185..>2805)
                     /locus_tag="VFX94_12925"
     CDS             complement(2185..>2805)
                     /locus_tag="VFX94_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5769130.1"
                     /translation="QGAIEGVAGPESANNAGAQNSFIPLLTLGIPPNAVMALMVGAMT
                     IHGLIPGPQVITKYPNLFWGMIASMWIGNLLLLIINLPLIGLWVRLLKVPYRLMFTTI
                     LLFCAIGIYSINNNPYDVYFTAFFGLVGYVFIKLGLETAPLLLGFVLGRLMEEKMRQA
                     LALSEGSFMTFLERPVAAVLLALALGIILIAVLPAVSRSRQKAFQE"
BASE COUNT          477 a          899 c          910 g          519 t
ORIGIN      
        1 cttgccgaac acctccgcca catcggcgcg cagcttgtgc accgccggaa cgacgatgtg
       61 gctgggcggt tcgtcgtcga gctgctggat gcgctcgccc aggtcggtct cgatgacctc
      121 gatgctgcgg gtctcgagga acggccgcat ctcgcattcg tcggtgagca tggatttgct
      181 ctttaccagg acccgaacgc ggttcttttc cagaaggtcg aaaacaatct ggttatgctc
      241 ggcggcgtcg cgcgcccagt gaacccgcat gccgttcgcg atcgcgttgc gctcgaactg
      301 ctcgaggtag tctgcgaggt gcgagagcgt gtgctccttg atggccgagg cgaggtttcg
      361 cagctcctcc cattcgggaa tggcgtgggc ctcgcggtcg cgcttctgcc tcaggtccca
      421 gagacgcttg tcatgaaatt ccgcgtgggt ggtcgacttg agaaaaaccg atgaagcttc
      481 agcatggtcc acgcgcttca tgcgcgcgcc ccgttcagca cctgcgcgat gtggatgaat
      541 ttgagcgcga ggccgagccg ctcggcgcag cccttctggt gcatcatgca cgaggggtcg
      601 ggcgtgacga tgtactcggc gcccgcggcg aggtggtcgt tcaccttgtc gtagcccatg
      661 cgcgccgaca ccggctcctc ggtgacggaa aatgtcccgc cgaagccgca gcattcgtcg
      721 ggcctcgcgg gtgtcacgat ctgcacgcct tttaccccgg agagaagagc aagcggcttt
      781 gagaaagccc gcccggcgat ctcgctcgtg ctcgcgaggc cgagaccgcg caggccggcg
      841 cagctcacgt gcaggccgac cttgtgcggg aacttcgccc acgggaactc gcgcacctgc
      901 agcacatcgt gcaggaactc ggtgagctcg agggcgttgg ggtgcagctt cgccctctcg
      961 cgcacatggt gcgtgcaact gcccgagggc gcgacgacat agtcgaacct ggcaaagttc
     1021 tttctaaaaa gccgctcggc cggcgccgcg tcgtcgtggc agccgctgtt cgccatcggc
     1081 tgcccgcaac acgtctggtc gagcggatag ccaacgtcga cccccagccg ctcgagcagc
     1141 tcaagggttg ctacgccgat ctcgggaaaa aaagtatcga cgtagcaggg gatgaaaagg
     1201 ccgatcttta ttactgcgcg tacgccttca gaatcgggtc ccagcgcttg acttcgctct
     1261 cgacgagctt ctgcaacgct tcgggcgatc gccccgcctt gtccgggatc acgctcccga
     1321 ggtcgagcag gcgcttgcgc gttgcttcgt cgtcaagcgc cttgaccatc gtctcgttca
     1381 accgcgtgtg gatttccctg ggtaacccct tgggcgcgaa gagcgcgttc caggcgctca
     1441 cctggtagtc gggcagcccc gcttccttgg tcgtcggcac gtcgggcagc gcgggcgagc
     1501 gctccgcggt ggcgatcgca taggccttga tgctgccgcc cttgacctgc tccaccaggt
     1561 tcacgatctg gtcgcacatg tagtccacct gcccgccgac gaggtcgttg agcgcgggcc
     1621 ccgtgccccg gtaggcggcg acgatgcttt tcgcgccgac ctgcgaactg agcagcgtgc
     1681 aggtcgtgaa ggacaccgac cccacgccgg cgtgcgcctg cttcaccttg gcgccggggt
     1741 ccttcaattt cgcgatgaac tccttcaggt tcgcggcggg aaagtccttg cgcgccacga
     1801 tcaggatcgg cgtgccggca acgaggccga taggcgtgaa gtccttggcc gggtcgtact
     1861 tcaggttctt gtacagcgcc ggcgccgcgc cgtgcgtccc catgtggccc atcatgatgg
     1921 tgtagccgtc gggcttcgcc tgcgcggccc gggtgatgcc ggtggtgccg cccgcgcccg
     1981 ccacgttctc cactacgaac tgctggccga gcgccttgga gaaatactcg ccggagatgc
     2041 gggcgatgat gtcggtcggc ccgcctgccg cgaacggcac gacgatggtc accgggcggg
     2101 ccggataagc ctgtgcaaac gacagtgagg cgaaaagcgc caaagcgcca gcgacggtga
     2161 aacgtttcat taatgtctcc ttggtcattc ctggaaagct ttctgccggc tgcggctcac
     2221 cgcgggcagc accgcgatca ggatgatccc gagcgcgagc gcgagcagca cggcggcgac
     2281 cgggcgctcg aggaaagtca tgaagctacc ctcggaaagc gcgagcgcct ggcgcatctt
     2341 ttcctccatc agcctgccga gcacgaagcc gagcagcagc ggcgccgtct ccagaccgag
     2401 cttgatgaag acatagccga ccagcccgaa gaacgcggtg aagtacacgt cgtacgggtt
     2461 gttgttgatc gagtagatgc cgatcgcgca aaagagcagg atcgtggtga acatcaggcg
     2521 gtacggcacc ttgagcagcc gcacccacag cccgatcagc ggcaggttga tgatgaggag
     2581 caggaggttc ccgatccaca tcgaggcgat cattccccag aacaggttcg ggtacttcgt
     2641 gatgacctgc gggcctggaa tcaggccgtg gatcgtcatc gcgcctacca tgagcgccat
     2701 caccgcattc ggtgggatgc cgagcgtgag cagcgggatg aatgaattct gcgccccggc
     2761 gttattcgcg ctttccggtc ccgccacgcc ctcgatcgcg ccctg
//
LOCUS       DASUNT010000642         3997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1309658, whole genome shotgun sequence.
ACCESSION   DASUNT010000642 DASUNT010000000
VERSION     DASUNT010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309658"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VFX94_12930"
     CDS             complement(<1..780)
                     /locus_tag="VFX94_12930"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5769131.1"
                     /translation="MTDLSPVSAAVGRLRPTFAGRLLQRNDAGYNEARRVHNGLIDKR
                     PALIAQCRGLADIVDAIQFARTQKLEVAVRGGGHNVAGRATIDGGVVIDMSLMKGIHV
                     DPKARTARAQGGLTWKDFNRETQLHGLATTGGVVSTTGIAGLTLGGGLGWLMGKHALA
                     LDNLISVDVVLADGRMLTASKEDNADLFWALRGGGGNFGVVTSFLFKLHPIPNLYAGP
                     MLWSLDRSAEVLRWYRDFITSAPDELNGFFAFLTVPAVAPFP"
     gene            complement(1162..1542)
                     /locus_tag="VFX94_12935"
     CDS             complement(1162..1542)
                     /locus_tag="VFX94_12935"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5769132.1"
                     /translation="MHRSRLGTVVIDCQTADLDRAAEFWAKALNRVARKPQDPADANY
                     RELETPPGEVVMAVQQVDHPSRVHIDIETDDLDAEVARLEKLGATRVAFVKRWWVMEA
                     PTGQRFCVVRPQRPDFPSGANDWS"
     gene            complement(1643..>3997)
                     /locus_tag="VFX94_12940"
     CDS             complement(1643..>3997)
                     /locus_tag="VFX94_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769133.1"
                     /translation="ILEAGRWLAAPWMNVIANAAFGFQAGVEGSGYTWSLGSRENQLT
                     PWSNDPVTDRPGEVLYVRDEASGELWGPTALPIRSGSAPYVAAHGQGYSRFEHSAHGV
                     ALELVQYVPLEDPIKISRLTLRNTSERTRRLSVTAYVEWVLGASRAASALSIVTEIDP
                     QTGALFARNPWNVAFGSRVAFADLGARQTAWTGDRREFLGRHGTLDSPAALAEEAPPL
                     SKRTGAGLDACGALQAPVVLEPGGTAEIVFFLGEAASDAEAQALVERYRTSDLDAVLR
                     EVAGFWDEVLGAIQVKTPDRSMDVMLNRWLLYQTLACRVWARAAFYQASGAYGFRDQL
                     QDGMALALSRPALTREHLLRAAGRQFVEGDFQHWWLPPSGQGVRTRISDDRVWLACAV
                     AHYVATSGDRAVLDERVAFLEGRPLAAGEHDAYFQPMLAEERASLFEHCARGLDRSLA
                     VGAHGLPLIGTGDWNDGMNRVGEHGKGESVWLGWFLHATLAAFAPLATARGEHARAAS
                     WLSNAAALKDALERNGWDGDWYRRGYFDDGTPLGASASEECRIDSIAQSWAVLSGAAD
                     PARARRAMAAVEEHLIRPSDGLALLFTPPFDRTPLDPGYIKGYPPGIRENGGQYTHAA
                     AWCVIAFAALGQGDRAAALFSLLNPINHAATRADVQRYKVEPYAVAADVYSVPPHVGR
                     GGWTWYTGSAGWLYRAGIESILGFRLQGTSFLLDPCIPPSWPRFELTYKHHSARYHIS
                     VENPRGVSRGVASATLDGNALPPGPARVPLVDDGAIYDVRVILG"
BASE COUNT          650 a         1294 c         1436 g          617 t
ORIGIN      
        1 tgggaagggc gccacagcgg gaacggtcag gaaggcgaag aacccattga gctcgtccgg
       61 ggcactggtg atgaagtcgc ggtaccagcg cagcacctcc gccgagcggt ccagcgacca
      121 gagcatcggt ccggcgtaga ggttgggaat cgggtgcaac ttgaagagga aggaggtcac
      181 cacgccgaag ttgccgccgc cgccgcgcag cgcccagaac agatctgcgt tgtcctcctt
      241 gctggccgta agcatcctgc cgtcggccaa gacgacgtcg accgagatga gattgtccag
      301 cgcgagcgcg tgcttgccca tgagccagcc gaggccgcct ccgagcgtga ggcccgcgat
      361 gcccgtggtc gagaccacgc ctccagtcgt cgcgagacca tggagctgag tttcacggtt
      421 gaagtctttc catgtgaggc ccccttgcgc gcgggccgta cgggccttgg gatcgacatg
      481 aatgcccttc atcagcgaca tgtcgatcac cacgccaccg tcgattgttg cgcgcccggc
      541 cacgttgtga ccgccgcctc ggaccgcgac ttcgagcttc tgcgtccgtg cgaattggat
      601 ggcgtccacg atgtcggcga gccctcggca ctgggcgatc agagcaggcc gcttgtcgat
      661 gagtccgtta tggactctcc tggcctcgtt atagccagcg tcattgcgct gaaggagacg
      721 tcccgcaaac gtcggacgca atctaccaac ggcggccgaa acgggtgaaa ggtcggtcat
      781 gtcagaactc ctgattcaag ggaggattca attcaactcg gactgtgagc ctcatacctt
      841 cgactcactg atccctaagg ttcagcggag cgtccgagct gggagtcgca cgagtcgggg
      901 ccgctaggtg tccggcaatc ctacgccaaa ctcagcatgc gatgcgcgtt ttaggggttc
      961 cgccgaactt atgctgcgga gactgttggc gcccaacgct cggacgccgt acgtccgcat
     1021 ggtcagaagc attgccgtct atctctcgcg tcgtgcagct cggcctgatg cggacgaagg
     1081 cgccgttcac ggcagggctc gccgagcggc ttgcacgcga gctccgcgta gcagttgatt
     1141 tgccgcactc atcacagcgc cctaggacca gtcgttcgcg ccgctcggga aatcggggcg
     1201 ctgtggccgc acaacgcaga agcgctggcc ggtgggcgct tccatcaccc accagcgctt
     1261 gacgaaagcc acgcgcgtcg cgccgagctt ctcgaggcgc gccacttcgg cgtcgaggtc
     1321 gtcggtctcg atgtcgatgt gcacgcggct cggatgatcc acttgttgca ccgccatcac
     1381 gacctcgccg ggaggcgtct cgagttcgcg gtagttcgcg tcggccggat cctgcggctt
     1441 gcgcgcaacg cggttaagcg ctttggccca gaattcggcc gcgcggtcga gatccgcggt
     1501 ttggcaatcg atgaccaccg tacccagacg gcttcgatgc atggatggac tccttcgtaa
     1561 ccgcgccacc ctactacgag aaccgcgtgg gatcagaaga aaagggggtg aacttgctga
     1621 ctggccagtc tttcccgccg agtcagccca ggatcacgcg cacgtcgtag attgcaccgt
     1681 catctacgag ggggacacgg gccgggccgg gggggagggc gttgccatcc aaggtggcgc
     1741 tggcgacgcc gcggctgacg ccgcgcgggt tctcgacgct gatgtggtag cgggcggagt
     1801 ggtgtttgta ggtgagctcg aagcgcggcc aggacggggg gatgcagggg tcgaggagaa
     1861 atgaggtgcc ctgcaggcga aagccgagga tggattcgat gccggcgcgg tagagccaac
     1921 cggcggagcc ggtgtaccag gtccagccgc cgcggccgac atggggaggc acggaataga
     1981 cgtcagcggc gacggcgtag ggctcgacct tgtagcgctg gacgtctgcg cgcgtggcgg
     2041 cgtggttgat gggattcagc aaggagaaga gggcggcggc ccggtcgccc tggccgaggg
     2101 cggcgaaggc gatgacgcac caggccgcag cgtgggtgta ctggccgccg ttttcgcgga
     2161 tgccgggcgg gtagcccttg atgtagccgg gatcgagcgg ggtgcggtcg aagggcggag
     2221 tgaagaggag cgcgaggccg tcactggggc ggatgaggtg ctcctccacc gcggccatgg
     2281 cgcgccgcgc gcgagccggg tcggcggcgc cggagaggac ggcccaggac tgggcgatgg
     2341 agtcgatgcg gcattcctcg ctcgcggagg cgccgagcgg ggtgccgtcg tcgaagtaac
     2401 cgcgccggta ccagtcgccg tcccagccgt tgcgctccag cgcatctttc agcgcggcgg
     2461 cgttcgaaag ccagctcgcg gcgcgcgcgt gctcgccgcg tgccgttgcg agcggggcga
     2521 aggcggcgag ggtcgcatgc aggaaccagc cgagccagac gctctcgcct ttgccgtgct
     2581 cgccgacgcg gttcatgccg tcgttccagt cgccggtgcc gatgagcggc aggccgtgcg
     2641 cgcccacggc gaggctgcgg tcgagcccgc gcgcgcagtg ctcgaagagc gaagcgcgct
     2701 cctcggcgag catgggctgg aagtaggcgt cgtgctcgcc cgccgcgagc ggccgacctt
     2761 cgaggaaggc aacgcgttcg tcgagcaccg cgcggtcgcc gctcgtcgca acgtagtgcg
     2821 ccacggcgca ggcgagccag acccgatcat ccgagatgcg cgtgcgcacg ccctggccgg
     2881 acggcggcag ccaccagtgc tggaaatcgc cttcgacgaa ctgccgtcct gccgcgcgca
     2941 ggaggtgctc gcgcgtcagc gccggccgcg acagggcgag cgccatgccg tcctggagct
     3001 ggtcgcggaa gccgtaggcg ccgctcgcct ggtagaacgc cgcgcgcgcc catacgcggc
     3061 aggcgagcgt ctggtagagc agccagcggt tgagcatcac gtccatggag cggtcgggcg
     3121 ttttcacttg gatcgcgccg aggacctcgt cccagaaacc ggccacctcg cgcaggaccg
     3181 catcgagatc gctcgtccgg tagcgctcga cgagtgcttg cgcttcggcg tcactcgccg
     3241 cctcgccgag gaagaacacg atctcggccg tgccgcccgg ctcgagcacc accggcgcct
     3301 gcagcgcgcc gcaggcatcg aggccggcgc cggtgcgctt ggagagcggc ggcgcctctt
     3361 cggcgagcgc ggccgggctg tcgagggtgc cgtgccggcc caggaattcg cgccggtcgc
     3421 cggtccaggc ggtctgccga gcgcccagat cggcaaaggc gacccgagag ccgaacgcca
     3481 cgttccaggg gttgcgcgcg aagagcgcgc cggtctgcgg atcgatctcg gtcacgatcg
     3541 agagcgccga cgccgcacgt gatgcgccga gcacccactc gacataagca gtgaccgaaa
     3601 gccggcgcgt gcgctccgac gtattgcgca gggtgagccg cgagatcttg atcggatcct
     3661 ccagcggcac gtactggacg agctcgagcg cgacgccgtg cgcgctgtgc tcgaagcggc
     3721 tgtagccctg gccgtgcgcg gcgacgtagg gcgccgagcc gctgcggatc ggcagcgcgg
     3781 tcggccccca gagctcgccg ctcgcctcgt cgcgcacata gaggacctcg cccggccggt
     3841 cggtcaccgg atcgttcgac cagggggtga gctggttctc gcggctgccg agcgaccagg
     3901 tgtagccgct gccttcgacg cccgcctgaa agccgaacgc ggcattggcg atgacgttca
     3961 tccacggcgc cgcaagccag cgcccagcct cgaggat
//
LOCUS       DASUNT010000643         3089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1221440, whole genome shotgun sequence.
ACCESSION   DASUNT010000643 DASUNT010000000
VERSION     DASUNT010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221440"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFX94_12945"
     CDS             <1..515
                     /locus_tag="VFX94_12945"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5769134.1"
                     /translation="GEAPRPLVWVGIVLICGGVLSLGLAGFHWRTSRVSTLWALANAV
                     IIAAYTLVDAGGVRAASSPATYVVWLFVLDALPFPLLVLAIKKDQLMVYASRYWLRGL
                     IGGALSAAAYGVVLWAMTRAPVAAVAALRETSVIFAALIGAWLLKEGHLARRVAGAAT
                     VAAGVVVLKI"
     gene            512..2779
                     /locus_tag="VFX94_12950"
     CDS             512..2779
                     /locus_tag="VFX94_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5769135.1"
                     /translation="MNAEIVIIGGGIVGASVAYHLAKLGRRDIVLLEQGRLTCGTTWH
                     AAGLVGQMRPNRSMTRMSQYGIQLYSTLEEETGLATGWKQCGSVNVARTPERWIVFQR
                     QAAMARSFGIEVHLLSPREALEKWPLLRIDDLQGALWFPADGKANPADLAQSLAKGAR
                     NLGVKIIEGVNALEVRAKNQFVSGVETSEGPISCDIAVNCAGQWARAFAGRQGINIPL
                     HSAEHFYIVTEAIAGVHPMLPVMRDPDGFIYFKEEVGGLLMGGFEPVAKPWGMEGIPD
                     RFEFQLLPEDWDQFQPLMEAAIHRVPALEEAGVKMLLNGPESFTPDGNFILGEAPELR
                     NYFVAAGFNSAGIANAGGAGKLLAEWIDGGESPMDLFEVDVRRFGAFAADAKWLKQRT
                     VETLGLHYAMRWPRHELESGRPLRVSPLYEVLKKKGAQFGSKFGWERANYFGAPMQYT
                     LGRPNWLGAVIEEQLAIRNAVGILDQTSFGKLLVRGRDARLLLDRVCANRIGELSYTP
                     MLNRKGGYESDVTVQRLDEEQYLVVTGSAQPVRDRAWLLRNRIAGEDVAVDDVTERWS
                     VISILGPRAKAVLDNTNIGAARAMPASYVGGPGWEIFVAIEEAAGLYEGLWNAGAALG
                     MRDCGYYALDALRIEAGRRAFGAELSPDCTPYEAGLGFTVRSERRKAPQALSKRLLMF
                     TFPASELFAWGGEPILREGKPVGELSSVGYSATVSCMVGMGFVHCGDIEGSYAIEVSG
                     QQVPAKASLQAPWRA"
     gene            2767..>3089
                     /locus_tag="VFX94_12955"
     CDS             2767..>3089
                     /locus_tag="VFX94_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769136.1"
                     /translation="MARLVRADRELELPRVDAALRADGHELILLPEGVAEQELAAATE
                     LAELLLMCYTPVTRRVIENARELRAIVKYGVGIDAIDLAAARDRGIPVVNVPEYAEHT
                     VAEGA"
BASE COUNT          474 a         1005 c         1098 g          512 t
ORIGIN      
        1 tcggcgaggc gccgcggcct ctcgtctggg tggggatcgt gctgatctgc ggcggcgtgc
       61 tgtcgcttgg cctcgcgggt ttccactggc gcacctcgcg cgtttccacg ctgtgggcgc
      121 tcgcgaacgc cgtcatcatc gccgcctaca ctctggtcga cgctggcggc gtgcgcgcag
      181 ccagcagccc ggcaacctac gtggtgtggc ttttcgtact ggatgcgctg cctttcccgc
      241 ttcttgtcct cgcgatcaag aaggaccagt tgatggttta cgcgagccgt tactggctgc
      301 gcgggctcat cggcggtgcg ctgtcggcgg cggcgtatgg cgtcgtgctg tgggccatga
      361 cgcgcgcacc ggtggcggcg gtggcagcgc tgcgcgagac ttcggtgatc ttcgccgcgc
      421 tcatcggtgc gtggctgctc aaggaagggc atctggcgcg ccgcgtcgcg ggcgcggcca
      481 ccgtagctgc cggcgtggtc gtattgaaaa tatgaacgcc gaaatcgtaa tcatcggcgg
      541 cgggatcgtg ggcgcaagcg tcgcctacca cctcgcaaag ctcggccgcc gcgacatcgt
      601 gctgctcgag caggggcggc tgacgtgcgg cacgacgtgg cacgcggccg ggctcgtggg
      661 gcagatgcgc cccaatcggt cgatgacccg catgagccag tacggcatcc agctctattc
      721 caccctcgag gaggaaaccg ggctggcaac cggctggaag cagtgcggga gcgtgaacgt
      781 ggcgcgcacg cccgagcgct ggatcgtgtt ccagcgccag gccgcgatgg cgcggagctt
      841 cggcatcgaa gtccatctgc tgagcccgcg cgaggcgctc gagaagtggc cgctcctgcg
      901 catcgacgac ctgcagggcg cgctttggtt ccctgccgac ggcaaggcga atcccgccga
      961 tctcgcgcag agcctcgcca agggggcgcg caacctgggg gtgaaaatca tcgagggcgt
     1021 gaatgcgctc gaggtgcggg ccaagaacca gttcgtgagc ggcgtggaga cgagcgaagg
     1081 cccgatctcg tgcgacatcg cggtcaactg cgcgggccag tgggcgcgcg cattcgccgg
     1141 ccggcagggc atcaacattc cgctgcattc cgccgagcac ttttacatcg tcacggaggc
     1201 gatcgccggc gtgcatccga tgctgccggt gatgcgcgac cccgacggct tcatctactt
     1261 caaggaggag gtcggcgggc tcctcatggg cggtttcgag ccggtcgcga aaccctgggg
     1321 catggagggt atcccggaca ggttcgagtt ccagctcctg cccgaggact gggaccagtt
     1381 ccagccgctc atggaggccg cgatccaccg cgtgcccgcg ctcgaagagg cgggagtgaa
     1441 gatgctgctg aacgggccgg agagcttcac gccggacggc aactttatcc tcggcgaggc
     1501 gcccgagctg cgcaattact tcgtcgccgc gggcttcaac tccgcgggca tcgccaacgc
     1561 cggcggcgcc ggcaagctcc tcgccgagtg gatcgacggc ggcgagtcgc ccatggacct
     1621 cttcgaggtc gacgtgcggc gcttcggcgc cttcgccgcc gatgcgaaat ggctgaagca
     1681 gcgcacggtc gagaccctcg gcctgcacta cgccatgcgc tggccgcgcc acgagctcga
     1741 atcgggacgg ccgctgcgcg tttccccgct gtatgaagtc ctgaagaaga aaggcgccca
     1801 gttcggctcg aagttcggct gggagcgcgc gaactatttc ggcgcgccga tgcagtacac
     1861 gctcgggcgg ccgaactggc tcggcgccgt gatcgaggag cagctcgcca tccgcaacgc
     1921 ggtcggcatc ctcgaccaga cctcgttcgg caagctgcta gtgcgcgggc gcgatgcgcg
     1981 gctgctgctc gatcgggtgt gcgcgaatcg catcggcgag ctcagctata cgccgatgct
     2041 gaaccgcaaa ggcggctacg aaagcgatgt cacggtccag cgcctggacg aggagcaata
     2101 cctggtcgtc acgggctccg cccagccggt gcgcgacagg gcgtggctct tgaggaacag
     2161 gatcgccgga gaggatgtcg ccgtcgacga tgtgaccgag cgctggtcgg tcatctcgat
     2221 cctcgggccg cgggcgaagg ccgtcctcga caataccaac atcggagccg cgcgcgcgat
     2281 gccggcctct tatgtcggcg gccccggttg ggagatcttc gtcgcgatcg aggaagccgc
     2341 ggggctctac gaaggtctct ggaacgccgg cgccgcgctc ggcatgcgcg actgcggcta
     2401 ctacgcgctc gacgcgctgc gcatcgaggc cggccggcgc gccttcggcg ccgagctctc
     2461 gcccgattgc acgccgtatg aagcagggct tggttttacc gtgcgttccg aaagacggaa
     2521 agcgccgcag gcgctgtcca agcggctgct catgttcacc ttcccggcga gcgagctctt
     2581 cgcctggggc ggcgagccga tcctgcgcga gggcaagccg gtgggcgagc tttcctcggt
     2641 cggctacagc gccaccgttt cctgcatggt cggcatgggg ttcgtccact gcggggacat
     2701 cgaaggcagc tacgccatcg aagtgtcggg acagcaggtt ccggcgaaag cgtcgctgca
     2761 ggcgccgtgg cgcgcttagt tcgcgccgac cgcgagctcg agctgccgcg ggtggatgcc
     2821 gccttgcgcg ccgacggtca tgagctgatc ctgcttcccg agggcgttgc ggagcaggag
     2881 ctcgcggcgg cgacggagct cgccgagctg ctgctcatgt gctacacgcc ggtcacgcgt
     2941 cgggtcatcg aaaacgctcg cgagctgagg gccatcgtga agtacggcgt cggcatcgat
     3001 gcgatcgacc tcgccgcggc gcgcgatcgc ggcatcccgg tcgtcaacgt ccccgaatat
     3061 gccgagcaca ccgtggccga aggcgcgtt
//
LOCUS       DASUNT010000644         2170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_607084, whole genome shotgun sequence.
ACCESSION   DASUNT010000644 DASUNT010000000
VERSION     DASUNT010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607084"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFX94_12960"
     CDS             complement(<1..328)
                     /locus_tag="VFX94_12960"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX5769137.1"
                     /translation="MNVAPPKTLGELFLGFLSIGARSFGGVMPWAHRVMVEERRWMTE
                     ADFTETIGLCQFLPGPNIGNASIVLGKRWFGLRGSVVAFLGLFALPFVWVMALGLLYA
                     DFSSIPA"
     gene            378..1193
                     /locus_tag="VFX94_12965"
     CDS             378..1193
                     /locus_tag="VFX94_12965"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5769138.1"
                     /translation="MRALLLALIGIPLFVQAQPLPIFDAHIHYSHDAWDVVPVQDAIA
                     IMRKAGVKRALVSSSGDDGQQRLFAAAPDLVIPELRVYRNRGETGTWFRDETLIPYLE
                     ERLRRYRYVGIGEFHLYGADADLPVPRRMVGLAKKYNLFLHAHSDIDAVERLFRQWPQ
                     ARILWAHAGFERPEKVAEMLRKHKNLWADLAFRTDHASGGKVDPSWRGVFLQFPDRFM
                     VGTDTFTPERWHYIPEHADWSRTWLADLPREVAERIAWKNGETVFGSLLQRPD"
     gene            1262..>2170
                     /locus_tag="VFX94_12970"
     CDS             1262..>2170
                     /locus_tag="VFX94_12970"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HEX5769139.1"
                     /translation="MLAFQASPEVGKFFSLELAVCAKAGAAAESVKVDAHMPEHRHGM
                     NYAPEVKPLGAGRWRAEGLMFHMPGKWEFVFEIRAAGTTDRMSHGVVLAEFSEEEKRK
                     ILSHGPWPPPARNDPSNRVSGKREAIAFGEKLFFEPRLSGTGSVLCATCHVPFRAFQD
                     SRPRGFGLAEVERNTPSLFNTRFHRWYGWDGAHDSLWSQSVRPLLDAREMNASAAHVA
                     GVVRKLFSKEYEAAFARPVPGEDEAVFVDVGKALAAFQETLVSGRTAFDDFRDALAAG
                     KDSSYPEPAKRGLRLFVGKGNCSVCHV"
BASE COUNT          368 a          709 c          744 g          349 t
ORIGIN      
        1 cggccgggat cgacgagaag tccgcgtaga ggagaccgag cgccatcacc cagacgaaag
       61 gcagcgcgaa gaggccgagg aaggcgacga ccgatccgcg cagcccgaac cagcgcttgc
      121 cgaggacgat cgatgcgttg ccgatgttcg gcccgggaag gaactggcac aggccgatgg
      181 tctcggtaaa gtcggcctcg gtcatccagc gccgctcctc caccattacg cggtgcgccc
      241 acggcatgac gccgccgaag gagcgcgcgc cgatcgagag gaagccgagg aagagctcgc
      301 cgagcgtttt cggcggcgcg acgttcaagg gatccaagcc tggtgacgtt atttcacggg
      361 gattctacac ttcggggatg cgcgccctcc tgctggcatt gatcggcatt ccactcttcg
      421 tccaggcgca accgctgccc atctttgacg cacacatcca ctacagccac gacgcctggg
      481 acgtggtgcc ggtgcaggac gcgatcgcga tcatgcgcaa ggccggcgtg aagcgcgcgc
      541 tcgtctcgag ctcgggcgac gacgggcagc agcgactgtt cgccgccgcg cccgatctcg
      601 tgatcccgga gctgcgcgtc taccgcaatc gcggcgagac cgggacgtgg ttcagggacg
      661 agacgctgat cccctacctg gaagagcgcc ttcgcaggta ccgctatgtg ggcatcggcg
      721 agttccatct ctacggcgcc gatgcggacc tgcccgtgcc gcgccgcatg gtgggactcg
      781 cgaagaagta caacctgttc ctgcacgcac actccgatat cgacgcggtg gagcgcctgt
      841 tccggcaatg gccgcaggcg cgcatcctgt gggcgcacgc cggcttcgag cgccccgaga
      901 aagtcgccga gatgctgcgc aagcacaaga acctgtgggc ggatctcgcg ttccgcaccg
      961 accacgcctc gggcggcaag gtcgatcctt cgtggcgcgg ggtttttctc cagtttcccg
     1021 accgcttcat ggtcggcacc gataccttca cgcccgagcg ctggcactac atccccgagc
     1081 acgccgactg gtcgcgcacc tggctcgcgg acctgccgcg cgaggtcgcc gagcgcatcg
     1141 catggaaaaa cggcgaaacg gttttcggct cgctgttgca gcggccggac tagcgctcgg
     1201 cggagctgcc ggcgcctgcg tcccggcgct ggagggcact cgcctggaat ccgcgcggta
     1261 cgtgctggcg ttccaggcat ctcccgaggt cgggaagttt ttctcgctcg aactcgcggt
     1321 ttgcgcgaaa gccggcgcgg ctgcggaatc ggtgaaagtc gatgcgcaca tgcccgagca
     1381 ccggcacggc atgaactacg cgccggaggt aaaacccctt ggcgccggcc gctggcgcgc
     1441 cgaggggctg atgttccaca tgcccggaaa gtgggagttc gtgttcgaga tccgcgccgc
     1501 cggcacgacc gacaggatgt cgcacggcgt tgtgctcgcc gagttctccg aggaagaaaa
     1561 gcgaaagatc ctgtcgcacg ggccgtggcc gccgcctgcg cgcaacgacc cgagcaaccg
     1621 cgtctccggc aagcgcgaag cgatcgcgtt tggcgagaag cttttcttcg agccgcggct
     1681 ctcgggcacg ggctcggtgc tgtgcgccac ctgccatgtg ccgttccgcg ctttccagga
     1741 cagccggccg cgcggcttcg ggctcgcgga ggtcgagcgc aacacgccga gcttgttcaa
     1801 tacgcgcttt catcgctggt acgggtggga cggcgcgcac gacagcctct ggtcgcagag
     1861 cgtgcgcccg ctgctcgatg cgcgcgagat gaacgcgagc gcggcgcatg ttgcgggcgt
     1921 ggtgcgcaag cttttttcca aggagtacga ggcggcgttc gcacgccctg tgcccgggga
     1981 agacgaagcc gtgttcgtcg acgtgggaaa agcgctcgcc gcgttccagg agacgctggt
     2041 gagcgggcga accgcttttg acgacttccg ggacgcgctc gcagcgggaa aggacagctc
     2101 ctatccggaa ccggcgaagc gaggcctgag gctcttcgtc ggcaagggca attgctcggt
     2161 gtgccacgtg
//
LOCUS       DASUNT010000645         2072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_957665, whole genome shotgun sequence.
ACCESSION   DASUNT010000645 DASUNT010000000
VERSION     DASUNT010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957665"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1584)
                     /locus_tag="VFX94_12975"
     CDS             complement(13..1584)
                     /locus_tag="VFX94_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5769140.1"
                     /translation="MRALLAMIFAAAATAAASRVDAQVLRVVPHSNLIILDPIWTTQY
                     MARNHGYMIYDTLFGTDETSQVRPQMVDTWTVSPDRRLWSFTLRAGLQFHDGAPVTGE
                     DVIASLARWGKRDAMGQKLMTFVERMDAPGRDEFRIFLREPCGFVLEALGKPSSNVPF
                     IMPRRVAETPADKQIEDYVGSGPYVFKRDEFKPGDKAVYTRNTDYVPRKEAPSGTAGG
                     KRVYVERVEWNLALRDAQSQVNALARGEIDVIEHPAFESYEQLRLEPGIALVRKDPVG
                     AQYMCRFNHLHPPFDTPTVRRAALAAMQQEPFLRAQVGVKELYRTCASMFTCNTPYAS
                     AKGSEIQSKSNPKKARELLKSSGYDGTPVLILKPTDVAPLQKLPEVAAQLLRQAGFNV
                     DLQAMDWGTLASRRAKKEPPAQGGWNLFCTVWAAPDIWHPLGNAALGAAGDRSWFGWP
                     ADPELERLRDDFARATEESRKKALAEAIQARAFEIGTHAPLGEIVDRLATRENVTGWV
                     IGPGDVYWNVRKTGG"
     gene            complement(1611..>2072)
                     /locus_tag="VFX94_12980"
     CDS             complement(1611..>2072)
                     /locus_tag="VFX94_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEX5769141.1"
                     /translation="AVATLLHDLGLAKAFSGPLRFEVEGANAARAFARKEGIDDRRAQ
                     LIWDGVALNSTLSIALYKEAEVALCTAGIGLDWAGIGYDALTEAQMAAILDAFPRLDM
                     KRQFARATCRIVEERPGTTYDNFARDFGERFVPGYRRISTVDYLFNSPFKE"
BASE COUNT          323 a          703 c          675 g          371 t
ORIGIN      
        1 gctctaatcg cctcagccgc cagtcttcct cacgttccaa tacacgtcgc ccggcccgat
       61 cacccagccg gtaacgttct ctcgcgtggc gagccggtcc acgatttcgc cgagcggcgc
      121 gtgggtgccg atctcgaaag cgcgcgcctg gatcgcctcg gcgagcgcct tcttcctcga
      181 ctcctcggtg gcgcgcgcga agtcgtcgcg cagcctctcg agctcggggt cggccggcca
      241 gccaaaccag gatctgtcgc cggcggcgcc gagggcggcg ttccccagcg ggtgccagat
      301 gtcaggcgcg gcccacacgg tgcagaaaag gttccagcca ccctgcgcgg gcggctcctt
      361 cttcgcgcgg cgcgatgcca gcgtgcccca gtccatggcc tgcaggtcga cattgaagcc
      421 ggcctgccgc aggagctgcg ccgccacctc gggcagcttc tgaagcgggg cgacgtcggt
      481 cggcttgagg atcagcaccg gcgtgccgtc gtagcccgag ctcttcagca gttcccgcgc
      541 cttcttcggg ttcgacttcg attggatctc gctgcccttc gcgctggcgt agggcgtgtt
      601 gcacgtgaac atcgaagcgc acgtccggta cagctccttg accccgacct gggcccgcag
      661 gaaaggctcc tgctgcatcg ccgccagcgc cgcccggcgc accgtcgggg tgtcgaacgg
      721 cgggtgcagg tggttgaagc ggcacatgta ctgggcgccg accggatcct tgcggacgag
      781 cgcgatgccc ggctccagcc ttagctgctc atagctctcg aatgccggat gctcgatcac
      841 gtcgatctcg cccctcgcga gggcgtttac ctgcgattgc gcatcgcgca gcgccagatt
      901 ccattcgacg cgctcgacgt acacgcgctt gccgcccgcc gtccccgacg gggcttcctt
      961 gcgcggcaca tagtcggtgt ttctcgtgta aacggccttg tcgccaggct tgaactcgtc
     1021 ccgcttaaag acgtacgggc cgctaccgac gtagtcttcg atctgcttgt cggcgggcgt
     1081 ttcggccacg cgcctgggca tgatgaaggg cacgttcgat gagggcttgc cgagcgcctc
     1141 gagtacgaac ccgcaaggtt cgcgcaggaa gatgcggaat tcatcccgcc ccggcgcgtc
     1201 catgcgctcg acgaaggtca tcagcttctg ccccatggcg tcgcgttttc cccagcgcgc
     1261 gagggaagcg atcacgtcct cgcctgtgac tggtgctccg tcgtggaact gcagtcccgc
     1321 gcgcagcgtg aagctccata ggcggcggtc cgggctcacc gtccacgtgt cgaccatctg
     1381 cgggcggacc tggctcgtct cgtcagtgcc gaagagcgtg tcgtagatca tgtacccgtg
     1441 gttgcgggcc atgtactgcg tggtccagat cgggtcgagg atgatgaggt tggaatgcgg
     1501 gacgacacgc aagacctgcg cgtcgacccg cgatgccgcg gccgttgccg ccgctgcgaa
     1561 gatcatggca agcaaagcac gcacgcgaac cccctcccga gcgagtaatg ctactctttg
     1621 aacggtgaat tgaagagata gtccacggtt gagatccgcc ggtagcccgg cacgaagcgc
     1681 tcgccgaaat cgcgcgcgaa attgtcatag gtggtaccgg ggcgttcctc gacgatccgg
     1741 caggtggcgc gcgcgaactg ccgcttcatg tccaggcgcg gaaacgcatc gaggatcgcc
     1801 gccatctgcg cctcggtcag ggcgtcgtag cctatgccgg cccaatccag gccgatgcct
     1861 gccgtacaca acgcaacctc cgcctccttg tacaaggcga tggaaagtgt cgagttgagc
     1921 gcgacgccgt cccagatcag ctgcgcgcgg cggtcgtcga ttccctcctt gcgggcgaat
     1981 gcgcgcgcgg cattcgctcc ctcgacttcg aagcgcagcg gcccgctgaa ggccttggcc
     2041 aggccgagat cgtgcaggag ggtcgcgacg gc
//
LOCUS       DASUNT010000646         2636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_257959, whole genome shotgun sequence.
ACCESSION   DASUNT010000646 DASUNT010000000
VERSION     DASUNT010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257959"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VFX94_12985"
     CDS             <1..643
                     /locus_tag="VFX94_12985"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX5769142.1"
                     /translation="VGFSEDRMRRVAGLHEVERVFSLADELGVEGPIPPLKVVPDPNL
                     VARALDAFGVSRDKRKLAVQISARRPTQQWPTRHFVELIERLHALGIAVMLVWAPGPS
                     SHPRHPGDDEKAAAITAAFTTGAAPVAYRTERLAELIGALAACDAVVTSDGGAMHLAA
                     ALQKPIVCFFGDVPPEQWYPRGVPHRILRAASRQVADIPVDETVAALRELLAI"
     gene            complement(636..1856)
                     /locus_tag="VFX94_12990"
     CDS             complement(636..1856)
                     /locus_tag="VFX94_12990"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEX5769143.1"
                     /translation="MRALYTIALRLALPLILLRLWWRGRREPGYREHVEERLGVYAVD
                     KPEKAVWVHAVSVGEARAAAPLVRELQRLLPDHAMVMTCTTAAGRETLKQVYGESMTC
                     VFLPYDYPEAVKSFLQTFRPRLAVLMETELWPNLLAESARHGVPVALANARMSETSAR
                     GYRRWRLLTGPGIRSLAAVCAQSEADADRLRALGATRVVVTGNLKFDVALDEAQLAAG
                     REWRRRLGRPVLLLASTREGEEKLLLDEIPSPAAFLTIVVPRHPQRFDDVARWVDARR
                     TKVAMPKETDRVYLGDTMGEMAFYYAACDVAVIGGSFMPLGGQNLIEALAAGAPVVLG
                     PSMFNFAEATRLALEVGAAVQAGDAKGAIRQALELMARAQARCAMAQAGRALCEAHRG
                     STARHVEVLRALIR"
     gene            complement(1853..>2636)
                     /gene="waaC"
                     /locus_tag="VFX94_12995"
     CDS             complement(1853..>2636)
                     /gene="waaC"
                     /locus_tag="VFX94_12995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEX5769144.1"
                     /translation="AAMHRAVRRVIPVAIRRWRSGLWKPSVWSEIAAWRGQLCAERYD
                     AVIDTQSLVKSALVARLARGKRHGMDRTSAREWLASGFYDLRYPVPRELHAVQRNRIV
                     AASALGYEISGPADYGLRTPDTGKSGYIVLLTMTSRADKLWPEERWVELARDLGAPAV
                     LPWGSEAERARAERISAQIGGTVPPRRNLEQLARLFVNARAVVGLDTGLTHLAAALGV
                     PAVGIYCGSDPALTGLYGAPLARNAGGPGRPPSASEVLRLLP"
BASE COUNT          390 a          967 c          875 g          404 t
ORIGIN      
        1 cgtcggcttt tccgaagacc ggatgcggcg cgtggccggc cttcacgagg tggagcgggt
       61 gttttcgctc gccgatgagc tcggcgtcga aggcccgatc ccgccgctca aggtcgtgcc
      121 ggacccgaac ctcgtcgcgc gcgcgctcga tgccttcggg gtgtcgcggg acaagcgcaa
      181 gctcgccgtg cagatcagcg ctcgccggcc gacgcagcag tggccgacgc ggcatttcgt
      241 cgagcttatc gagcgcctgc acgcgctcgg cattgccgtc atgcttgtct gggcccccgg
      301 cccgtcgagc catccccgcc atcccggcga cgacgagaag gcggcggcga tcacggccgc
      361 ctttacgaca ggcgccgcgc cggtcgccta ccgcaccgag cggctcgccg agctgatcgg
      421 cgcgcttgcc gcctgcgacg ccgtcgtcac gagcgacggc ggcgccatgc acctggccgc
      481 cgcgctgcag aagcccatcg tgtgcttctt cggcgacgtc ccgcccgagc aatggtatcc
      541 ccgcggcgtg ccgcaccgca ttctgcgcgc agccagccga caggtggccg acatcccggt
      601 ggacgagacg gtggcggcgc tgcgggagct gctcgctatc tgatcagcgc gcgcaatact
      661 tcgacatgtc gagcggtcga gccgcggtgc gcctcgcaca aggccctgcc ggcttgcgcc
      721 atcgcgcaac gggcttgcgc gcgcgccatt agctcgagcg cctggcggat cgcccctttg
      781 gcatcgcccg cctggaccgc cgccccgacc tcgagggcga gccgcgtcgc ctccgcgaag
      841 ttgaacatgc tcgggccgag caccacgggc gcgccggctg ccagcgcctc gatgaggttc
      901 tgcccgccga gcggcatgaa gctgccgccg atcaccgcca cgtcgcacgc cgcgtagtag
      961 aacgccatct ctcccatggt gtcgccgagg tacacgcgat cggtctcctt tggcatggcc
     1021 acctttgtgc ggcgcgcgtc tacccatcgc gcgacgtcgt cgaagcgctg cggatggcgc
     1081 ggcaccacga tggtgagaaa cgcggctggg gacggtattt cgtccagcag cagcttttcc
     1141 tctccctcgc gcgtgctggc gagtaggagc accggccgcc cgagcctcct cctccattcg
     1201 cgccctgcgg caagctgcgc ctcgtcgagc gcgacgtcga acttgaggtt gccggtgacc
     1261 acgacacgcg tggcgccaag cgcgcgcaga cgatcggcgt cggcttcgct ctgggcgcaa
     1321 accgccgcga ggctccgaat cccgggcccg gtcagcaagc gccagcgccg gtagccgcgc
     1381 gccgacgtct ccgacatgcg cgcgttggcg agcgccaccg ggacgccgtg gcgtgcgctt
     1441 tcggcaagaa ggttgggcca gagctcggtt tccatgagaa ccgcgagcct cgggcggaaa
     1501 gtctggagga agctcttcac tgcttcgggg tagtcgtatg gcaggaaaac gcaagtcatg
     1561 ctttcgccgt agacctgctt gagcgtttcg cggcccgccg ccgtggtaca ggtcatgacc
     1621 atggcatggt cgggcagcag gcgctgcaac tcgcgcacca gcggcgcagc ggctctcgct
     1681 tcgccgaccg acaccgcgtg cacccagacg gccttttctg gcttatcgac cgcatacacg
     1741 cccaggcgct cctcgacatg ctcccgatag ccgggctcgc gccgcccgcg ccaccaaagg
     1801 cgcaggagaa tgagcggcag cgcgagccgc agcgcgatcg tgtagagcgc cctcatggca
     1861 ggagccgcag cacttcggac gcgctcggtg gccgccccgg gccgcctgcg ttccgcgcga
     1921 gcggcgcgcc gtacagcccg gtcagcgccg ggtcagagcc gcaatagatg ccgaccgccg
     1981 gaactccgag cgcagcagca aggtgcgtaa gcccggtgtc gaggcccacc acggcgcgcg
     2041 cgttgacgaa aagccgtgcg agctgttcca ggtttctcct cggcggcacg gtgccgccga
     2101 tctgcgccga gatgcgctcg gctcttgctc gctccgcctc gctcccccac ggaagtacgg
     2161 ccggcgcgcc aaggtctcgt gcgagctcga cccagcgctc ctccggccac agcttgtccg
     2221 cgcggctcgt catcgtgagg agcacgatat agccggactt gccggtatcc ggcgtgcgca
     2281 agccataatc cgccgggccg cttatctcgt atccgagcgc ggatgcggcg acgatcctgt
     2341 ttctctgcac ggcgtgcagc tcgcgcggca ctggatagcg caaatcgtag aacccgctcg
     2401 caagccattc gcgcgcgctc gttcgatcca tgccgtggcg cttgccgcgc gcgagccgcg
     2461 cgaccaacgc gctcttcacg agcgattgcg tgtcgatcac cgcgtcataa cgctcggcgc
     2521 aaagctgccc gcgccacgcg gcgatctccg accacaccga gggcttccac agccccgagc
     2581 gccagcgccg aatcgcgacc ggtatcacgc ggcgcacagc gcggtgcatc gccgct
//
LOCUS       DASUNT010000647         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_695929, whole genome shotgun sequence.
ACCESSION   DASUNT010000647 DASUNT010000000
VERSION     DASUNT010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695929"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VFX94_13000"
     CDS             <1..503
                     /locus_tag="VFX94_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006762484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5769145.1"
                     /translation="LPLGAGSFELVWSNMALHWLADPLSAFREFARVLAPEGLLMFST
                     LGPDTLKELRAATAAERVHEFIDMHDLGDMLVAAGFAAPVMDMEMIQVEYGKSENLLA
                     DLRASGQTSARADRRRGLGGRAFRERLRAALPGRATFEVVYGHAWKGRKADPEVKSVR
                     IFKRMP"
     gene            540..980
                     /locus_tag="VFX94_13005"
     CDS             540..980
                     /locus_tag="VFX94_13005"
                     /inference="COORDINATES: protein motif:HMM:NF021517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HEX5769146.1"
                     /translation="MDTPQFSRTLKRNCSMSPADLAKVFAALAALVLAIGAGFAAAGA
                     WLVLPFAGLEVLLLGAAFVLHARHAADYERIELQSGRLEVEVTEADRVARYQLQNARV
                     SMEEGRVVLRDAKEEIEIGRHIGAEARAELVAELEKTLIQGRIA"
     gene            1076..2031
                     /gene="coxB"
                     /locus_tag="VFX94_13010"
                     /pseudo
     CDS             1076..2031
                     /gene="coxB"
                     /locus_tag="VFX94_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009970051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
BASE COUNT          346 a          652 c          709 g          324 t
ORIGIN      
        1 gcctgcccct cggcgccggg tcgttcgagc tcgtgtggtc gaacatggcg ctgcactggc
       61 tcgccgaccc gctgtcagcg ttcagggaat tcgcgcgcgt gctggcgccc gaggggttgc
      121 tcatgttcag cacgctcggg cctgacaccc tgaaggagct gcgcgctgcc acggcggccg
      181 agcgggtgca cgagttcatc gacatgcacg acctcggcga catgctggtc gcggcggggt
      241 ttgctgcccc cgtcatggac atggagatga tccaagtcga atacggaaag agcgagaatc
      301 ttctggccga cctgcgcgcg agcgggcaga cgagcgcccg cgccgaccgg cggcgcggcc
      361 tgggcgggcg cgcgttccgc gagcggctgc gcgcggcgct gccggggcgc gccacgttcg
      421 aagtggtgta cgggcacgcg tggaaaggcc gcaaggcaga cccggaagtg aaaagcgttc
      481 gtattttcaa gcgcatgcct tgaggcgcga gttccggttg tgatactttc gcctttttgg
      541 tggacacccc gcaattcagc cggactctga agcgcaattg ttcgatgtcc ccggctgatc
      601 tggcgaaggt gttcgcggcg ctcgcggctc tcgtgctcgc gatcggcgcc ggcttcgccg
      661 ccgcgggagc gtggctcgtc ctgccgttcg ccgggcttga agtgctgctg ctcggcgcgg
      721 cattcgtgct gcacgcgcgc catgcggccg attacgagag gatcgagctg cagtcgggtc
      781 gcctcgaggt cgaggtgacg gaggcggacc gcgtcgcgcg ctaccagctg cagaacgcca
      841 gggtatcgat ggaagaaggg cgcgtcgtgc tgcgcgatgc gaaagaagaa atagagatcg
      901 gtcggcatat cggcgccgaa gcgcgcgccg agctcgtggc cgaactggaa aagacgctca
      961 ttcagggaag aattgcatga agggaagcaa gctcgtctca cgcacactcg ctgtgttcgc
     1021 cggattggcc gcagcagccg cttcaggagc tgcgcacgcg atcgcttggt attttcagac
     1081 gcccgcgtcg aagatggcgc aggacatcga cacgctgcac caggtgatca tgtggatcat
     1141 cgtggtgatc ttcgtcggcg tgttcgggtt catgttctac gcctgctacg cgcaccgcaa
     1201 gtcgatcggg cacaaggccg cccagttcca cgagaacacc accgtggagc tgctctggac
     1261 cgtgatcccg gcgctgattc tgatcgtcat cgcgtggccg gtgaccaagg tggtgatcgc
     1321 gcagaaggac acctccgccc ccgacctcac catcaaggtg accggctatc agtggaaatg
     1381 gggctacgac tatctgcggg gcgaaggcga aggcatcagc ttcacctcca tgcttgccac
     1441 gccgcgcgag cagatcgacg gcaaggcgcc caagggcgag aactatctgc tcgaggtcga
     1501 ccacgagatg atcgtgccgg tcggcaagaa gatccgcgtc atcaccaccg ccgccgacgt
     1561 ggtccacgcc tggtggatcc cagctctggg cgcgaagcag gacgcgatcc ccggcttcct
     1621 gcgcgacacc tggttccgcg ccgagcaggc gggcacctac cgcggccagt gcaccgagct
     1681 ctgcggcaag gagcacgcct tcatgccgat cgttgtgcgc gtcgtgccgc aggacgagta
     1741 cacgaagtgg gtgggcgagc gcaagaagat cgctgccggc gtcgaggcgc cggcgccggt
     1801 cgcgtctgcc gctccggcgg cggccgccgc gcccgccgac gagagcaagc cgctgccgct
     1861 ggccgagctc gtgagccgcg gcgaaaagat ctacgccgcg aactgcatcg cctgccacca
     1921 ggcgaccggg cagggcacgc cggcgatgaa ggcgcccgcg ctcgcgggca acaagctggt
     1981 caccggcgcc gagcaggcgg gcacctaccg cggccagtgc accgagctct g
//
LOCUS       DASUNT010000648         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1222316, whole genome shotgun sequence.
ACCESSION   DASUNT010000648 DASUNT010000000
VERSION     DASUNT010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222316"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /gene="mutM"
                     /locus_tag="VFX94_13015"
     CDS             complement(<1..569)
                     /gene="mutM"
                     /locus_tag="VFX94_13015"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX5769147.1"
                     /translation="MPELPEVEVTRRGLAPQITGRTISAVAVREPRLRWPVPREVLTL
                     AGRTVRNIRRRGKYLLVDCGDGHLILHLGMSGSLRVLPPDTPAQKHDHFDLVLGDRLL
                     RLRDPRRFGAVLWVSGDTDAHPLLAHLGIEPLSRSLDAKRLYALTRAHRTAIKQFLMD
                     GRRIVGVGNIYAAESLFRAGIDPRKPARRL"
     gene            635..2284
                     /locus_tag="VFX94_13020"
     CDS             635..2284
                     /locus_tag="VFX94_13020"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5769148.1"
                     /translation="MHKLIVAGALGCACVSACAQPAEDLSEPTLYEFLLGEIAVQRGD
                     LPLAAKTYLDLARRTRDPRVARRAVEVANQAKETGVALQAARLWNELEPSAHSLQVLA
                     ATLVAAKRVDEAGPVLEKLLSAEGVNRENAFMQLNRLLAGNPDKMANQRVVRNIAAKY
                     PQLAQAHFALAQAAAIAGDNETALAAAREAQKLQPDWELAVLLEAQVLQQRSPAAAAK
                     RLGEFVQAYPSAREARLSYARVLVLEKRLPEARKQFEAVASASPKNADVVYAVGLLAF
                     QLKDYTAAEEYMKRVLGLGYRDPEGVRYLLGQIAEEQKLWPRAIQWYETIETGDHVLP
                     ARMRTASAMAKQGQLDEARAFLKRVADENPEDSVQLTVAEAQLLRDAQRHAEAFTLLS
                     EALGKQPEQPDLLYDLALTAEKLERYDILESHLRKLIEVKPDHAHAYNALGYSFADRN
                     TRLPEARKLIEKALELAPEDYFIMDSMGWVLYREGDLKGAARELRRAYSGRPDAEIGA
                     HLGEVLWMLGERDEARRVWDEALKAGPENETLQKTIKRLRR"
BASE COUNT          411 a          800 c          833 g          328 t
ORIGIN      
        1 agccggcgcg cgggtttgcg agggtcgatg cctgcgcgga acaggctctc ggctgcgtag
       61 atattgccga cgccgacgat acgccggccg tccatcagga actgcttgat cgccgtgcga
      121 tgcgcacggg tcagcgcgta caggcgcttc gcgtcgagcg agcgcgacag cggctcgatg
      181 cccagatgag cgagcagcgg atgcgcgtcg gtatcgcctg acacccatag caccgcaccg
      241 aagcgccgcg ggtcgcgcag ccgcagcagg cggtcgccta gcacgagatc gaaatgatcg
      301 tgcttctgcg ccggcgtgtc cggtggcagc acgcgaaggc ttccggacat gccaaggtgc
      361 agaatgagat ggccgtcgcc gcaatccacg agcagatact tgccgcgccg gcggatgttg
      421 cgcacggtgc ggccggcaag cgtcaaaacc tcacgcggca ccggccagcg cagccgcggc
      481 tcgcgcaccg cgacggcgct gatcgtgcgg ccggtaattt gcggcgcgag gccgcggcgc
      541 gtcacttcga cttcgggaag ctccggcatg ccaagtgcct tgtgcggggc accaaatata
      601 cctaagatcg ctcggaccca gcatggtgaa agcaatgcac aaattgattg tggcgggagc
      661 gcttggctgc gcttgcgtca gcgcgtgcgc ccagccggcg gaggatctga gcgagcccac
      721 gctgtacgag ttcctgctcg gcgagatcgc cgtgcagcgc ggcgacctac cgctcgccgc
      781 caagacctac ctcgatctcg cgcggcgcac gcgcgatccg cgcgtcgcgc gccgcgcggt
      841 ggaggtcgcg aaccaggcga aggagaccgg agttgcgctg caggccgcgc ggctgtggaa
      901 cgagctcgag ccctcggcgc actcgctgca ggtgctggcc gcgacgctgg tcgcggcaaa
      961 gcgcgtcgac gaggccgggc cggtgctcga gaagctgctc tccgccgaag gcgtgaaccg
     1021 cgagaacgca ttcatgcagc tgaaccggct gctcgcgggc aatcccgaca agatggccaa
     1081 ccagcgggtg gtcaggaaca tcgccgccaa gtatccgcag ctggcgcagg cgcactttgc
     1141 gctcgcgcag gccgccgcga tcgccggcga caacgagacc gcgctcgccg ccgcgcgcga
     1201 agcgcagaag ctgcagcccg actgggagct cgccgtgttg ctcgaagcgc aggtgctgca
     1261 gcaacgttcg cccgcggcgg ccgcgaagcg gctcggcgag ttcgtccagg cctacccgag
     1321 cgcacgcgaa gcgcggctca gctatgcgcg cgtgctggtg ctggagaagc gcctgcccga
     1381 ggcgcgcaag cagttcgaag ccgtggcgag cgcatcgccg aagaacgccg acgtggtcta
     1441 tgccgtcggc ctgctcgcgt tccagctgaa ggattacacc gccgccgagg agtacatgaa
     1501 gcgcgtcctc ggcctcggtt atcgcgaccc cgagggcgtg cgctacctgc tcgggcagat
     1561 cgccgaggag cagaagctct ggccgcgcgc gatccagtgg tacgagacca tcgagacggg
     1621 cgaccacgtc ctgccggccc gcatgcgtac cgcgagcgcg atggcgaagc agggccagct
     1681 cgacgaagcg cgcgcgttcc tgaagcgcgt cgccgacgag aaccccgagg attcggtgca
     1741 gctgaccgtg gccgaggccc agctcctgcg cgacgcccag cggcacgcgg aagcgttcac
     1801 gcttctgtcc gaggcgctcg gcaagcagcc cgagcagccg gatttgcttt acgacctcgc
     1861 ccttaccgcc gagaagctcg agcgctacga tatcctcgag tcccatctgc gcaagctgat
     1921 cgaagtgaag cccgaccacg cgcacgcgta caacgcgctc ggctattcgt tcgccgaccg
     1981 caacacgcgg ctgccggagg cgcgcaagct gatcgagaag gcgctcgagc tcgcccccga
     2041 ggactacttc atcatggaca gcatgggctg ggtcctctac cgcgaaggcg accttaaggg
     2101 cgcggcgcgc gagctgcgtc gcgcctatag cgggcggccc gatgccgaga tcggcgcgca
     2161 cctcggcgaa gtgctctgga tgctcggcga gcgcgacgag gcccggcgcg tctgggacga
     2221 ggcgctgaag gccggcccgg agaacgagac gctgcagaag acgatcaagc ggctgcggcg
     2281 gtgagaagcg ttgtactcgc ggcgctgctt ctcgccgcgt gcgcgcagat cgagatcaag
     2341 ccgcccgaag gtccgctcga gttctcgctc gc
//
LOCUS       DASUNT010000649         3538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_258432, whole genome shotgun sequence.
ACCESSION   DASUNT010000649 DASUNT010000000
VERSION     DASUNT010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258432"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VFX94_13025"
     CDS             complement(<1..598)
                     /locus_tag="VFX94_13025"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5769149.1"
                     /translation="MALAILCWTPLFSVAKRTLPVLDAFALGTVRYALGIALFVLLLA
                     AFEGRSALRFGDRLGAAVLSGMIGITAFNLFVWLGLGYTRPEHASIILALQSPLTALA
                     LWLVRGQRPAGFTLACVAIAITGVVLVISKGDLANALAGGSLLGDALVFMGAISWVTY
                     ALSAGRFPGWSPLRFTVMTCIPGAVGLAIANAIALAIGV"
     gene            complement(647..2314)
                     /locus_tag="VFX94_13030"
     CDS             complement(647..2314)
                     /locus_tag="VFX94_13030"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEX5769150.1"
                     /translation="MERIWLKSYPAGVPADVDPKKYRSLIQLFDTSIAKYRDRPAFHS
                     MGKAITFGELDKLSRAIGAWLQARALAKGARVAIMMPNCLQYPIAMFGILRAGCTVVN
                     VNPLYTPRELEHQLSDAGAEAIVILENFGHVLQEVRLRTPLKHMVVTSLGEMLGFKGA
                     IVNLVVRKVKKMVPPYDLPGAISFKQALSAGASRSLSTPPLGHDDIAFLQYTGGTTGV
                     SRGAMLLHRNIIAALLQYEAWLGPAIGSERAVIITALPLYHIFSLTVNCLVMMVVGGE
                     NVLITNPRDIPGFVKELAKHKYTMITGVNTLFNALLNNPDFAKLDFSSLKLAVGGGMA
                     VQKAVAERWKQVTGTPLIEGYGLTETSPAATANPMNLTEFSGTIGLPMSSTEIVLRDD
                     SDRDVPLGQPGEICIRGPQVMAGYWQRPDETAKAIGKDGFFRTGDVGVMDAKGFITIV
                     DRKKDMILVSGFNVYPNEIEQVVAMHPGVLEVAAIGVPDEHSGEVPKIFVVKRDPQLT
                     EHDLIEHCKKELTGYKRPKYVEFRTELPKTNVGKILRRALREEKKAA"
     gene            complement(2394..3167)
                     /locus_tag="VFX94_13035"
     CDS             complement(2394..3167)
                     /locus_tag="VFX94_13035"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX5769151.1"
                     /translation="MTYEFITVEQKGRVGVITLNRPKQLNALSPGLMKELGQALAALD
                     ADAGIGAIVLTGNEKAFAAGADIAAMKDFSYMHAYLTDYISDWEHFRKVRKPVIAAVA
                     GYALGGGCELAMHCDIVIAAENARFGQPEVNLGIMPGYGGTQRLPRFTSKAKAMDLCL
                     TARMMDAPEAERAGLVSRVVASDKLMEEALAVGEKVAGCSLPVVMMIKESINRAYETS
                     LAEGVLFERRLFQSQFSLEDQKEGMAAFLEKRKPAFKNR"
     gene            complement(3157..>3538)
                     /locus_tag="VFX94_13040"
     CDS             complement(3157..>3538)
                     /locus_tag="VFX94_13040"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX5769152.1"
                     /translation="STRPGATFFIPGTRGAEIHALVRPTGEETVVEKNYPNSFRATAL
                     QQLLEGQGVKHLAVAGMMTHMCVDASVRHAADLGYKITLLGDACATRAQSYGGEKVPA
                     KQVHAAFLAALNGFYAKVIKTHDL"
BASE COUNT          586 a         1161 c         1173 g          618 t
ORIGIN      
        1 cgacgccgat ggccagggcg atcgcattgg cgatcgccag cccgaccgcg cccgggatgc
       61 aggtcatgac ggtgaagcgc agcggcgacc agccgggaaa gcggccggcc gagagcgcat
      121 acgtgaccca cgatatcgcg cccatgaaga cgagcgcatc gccgaggagg cttccgcccg
      181 caagggcgtt ggcgaggtct cctttggaga tcacgagcac cacgccggtg atcgcgatgg
      241 cgacgcacgc gagcgtgaag cccgccgggc gctgtccgcg caccagccag agcgcgagcg
      301 ccgttagggg cgactgcagc gcgaggatga tcgaggcgtg ctccgggcgc gtgtagccga
      361 gcccgagcca cacgaaaaga ttgaacgcgg tgatgccgat catgccagaa aggactgctg
      421 cgcctaggcg gtcgccgaaa cgcagcgcgc tgcggccctc gaaggcggcg agcagcagca
      481 cgaaaagcgc gatgccgagc gcatagcgca ccgtgccgag cgcgaaggcg tcgagcaccg
      541 gcagcgtgcg cttcgccacc gagaagagcg gcgtccaaca caggatggca agcgccatcc
      601 agccgaggcc gcggaccagg aaaaccgggg ccagaaacgc ttctgcctaa gctgccttct
      661 tctcctcgcg cagcgcgcgt cgcaaaatct tgccgacgtt ggttttcggc agctcggtgc
      721 ggaactcgac gtacttcggc cgtttgtagc cggtgagctc cttcttgcag tgctcgatga
      781 ggtcgtgctc ggtgagctgc ggatcgcgct tcacgacgaa gatcttgggc acctcgccgg
      841 aatgctcgtc gggcacgccg atcgccgcca cctcgagcac gcccgggtgc atcgccacca
      901 cctgctcgat ctcgttcggg tacacgttga agccggacac gaggatcatg tcctttttgc
      961 gatcgacgat cgtgatgaat ccctttgcgt ccatgacgcc gacgtcgccc gtgcggaaaa
     1021 agccgtcctt gccgatcgcc ttcgcggtct cgtccgggcg ttgccagtag cccgccatca
     1081 cctgcggccc gcggatgcag atttcgcccg gctggccgag cggcacgtcg cggtcgctgt
     1141 cgtcgcgcag cacgatctcg gtcgaggaca tcggcaggcc gatggtgccg ctgaactcgg
     1201 tgagattcat cggattggcc gtggcggcgg gcgaggtctc ggtgagcccg tagccttcga
     1261 tgagcggcgt gccggtgacc tgcttccagc gctcggccac ggccttctgc acggccatgc
     1321 cgccgccgac ggcgagcttc aggctcgaga aatcgagctt cgcgaaatcg gggttgttga
     1381 gcagcgcgtt gaagagcgta ttgacgccgg tgatcatcgt gtacttgtgc ttcgccagct
     1441 ccttgacgaa gcccgggata tcgcgcgggt tggtgatcag gacgttctcg ccgccgacaa
     1501 ccatcatcac caggcaattc accgtgagcg aaaagatgtg gtagagcggc agtgcggtga
     1561 tgatgaccgc gcgctcactg ccgatggccg gcccgagcca cgcttcgtac tggaggagcg
     1621 cggcgatgat gttgcggtga agcagcattg cgcccctcga cacgccggtc gtgccgccgg
     1681 tgtactgcag gaacgcgatg tcgtcgtgac cgaggggcgg agtcgagagc gacctggacg
     1741 cccccgcgga gagcgcctgc ttgaacgata tggcgccggg cagatcgtag ggcggcacca
     1801 tttttttcac cttgcgcaca accaggttga cgatcgcacc cttgaagccg agcatctcgc
     1861 cgagcgaggt aacgaccatg tgcttgagcg gcgtgcgcag ccgcacctcc tgcagcacgt
     1921 ggccgaaatt ttccaggatc acgatcgcct cggcacccgc gtcgctaagc tggtgctcaa
     1981 gctcacgcgg tgtgtaaagc ggatttacgt tcaccacggt acagccggcg cgcaggatgc
     2041 cgaacattgc gatcgggtac tgcaggcagt tcggcatcat gatcgcgacc cgcgccccct
     2101 tggcgagcgc gcgcgcctgc agccaggcgc cgatggcgcg cgacagcttg tcgagctcgc
     2161 cgaacgtaat cgcctttccc atgctgtgga acgccgggcg gtcgcggtac ttggcgatgc
     2221 tcgtgtcgaa gagctggatg agcgagcggt acttcttcgg atcgacgtcg gccgggacgc
     2281 cggcgggata gctcttgagc cagatgcgtt ccatgggtcc tcctccgcgg agcagcgatt
     2341 ctaccgggga ctgacccccg gttcccggtt tggggtctga ccccgacggc gggttacctg
     2401 ttcttgaacg cgggcttgcg cttctcgagg aacgctgcca tgccctcctt ctggtcctcg
     2461 agcgagaact gcgactggaa caagcgccgc tcgaagagca cgccctcggc gagcgaggtc
     2521 tcgtaggcgc ggttgatcga ctccttgatc atcatcacca ctgggagcga gcagcccgca
     2581 accttctccc cgaccgccag cgcctcctcc atgagcttgt ccgaggccac gaccctggac
     2641 accaggcccg cgcgctcggc ctccggcgcg tccatcatgc gcgccgtgag gcacagatcc
     2701 atcgccttcg ccttgctcgt gaagcgcggc aggcgctgcg tgccgccgta gcccggcatg
     2761 atgccgaggt tgacctcggg ctggccgaag cgcgcgtttt ccgcggcgat cacgatgtcg
     2821 cagtgcatgg cgagctcgca gcccccgccc agcgcgtaac ccgcgaccgc ggcgatcacg
     2881 ggcttgcgca ccttgcgaaa atgctcccag tcgctgatgt agtcggtgag ataggcgtgc
     2941 atgtagctga agtccttcat cgccgcgatg tcggcgccgg cggcgaacgc cttctcgttg
     3001 ccggtgagca cgatcgcccc tatgcccgcg tcggcatcga gcgcggcgag cgcctgtccc
     3061 agctccttca tcaggccggg gctcagggca ttcagctgct tcggccggtt gagcgtgatg
     3121 acgcccaccc ggcccttctg ctctaccgtg atgaattcat aggtcatggg tcttgatcac
     3181 ttttgcgtag aaaccgttga gcgcggcgag gaacgcggcg tgcacctgct tcgccggcac
     3241 cttctcgccg ccgtagctct gcgcgcgcgt ggcgcaggca tcgcccagca gcgtgatctt
     3301 gtagcccagg tcggcggcgt gacgcacgct cgcgtcgacg cacatgtgcg tcatcatgcc
     3361 ggcgaccgcg aggtgcttga cgccctgccc ttcgagcagc tgctgcagcg ccgtggcgcg
     3421 gaagctgttg ggatagtttt tttccaccac ggtttcttcc ccggtgggac gcaccagggc
     3481 gtgtatctcc gcgcccctcg tgccgggaat gaagaacgtc gcgcccggac gcgtcgac
//
LOCUS       DASUNT010000650         3316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_347175, whole genome shotgun sequence.
ACCESSION   DASUNT010000650 DASUNT010000000
VERSION     DASUNT010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347175"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VFX94_13045"
     CDS             <1..795
                     /locus_tag="VFX94_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX5769153.1"
                     /translation="PRVEATSYSNPKVVPQFADASELLKSIQRKSGVSYKATCANVRA
                     VERALADLEAGHGASEVSLLVSATESHTRKNLNRNRAEQWQNIAEMVAAANGRLRMVG
                     TISVAFGCPFEGSVDPGSVLADVERFARLGVAHVALGDTTGMATPPAVRKIFKSFPGS
                     VVGIAHVHATRGTGLVNCVAAYEAGVRHFDSSFGGVGGHPAKVQYGGGFTGNVCTEDL
                     VNLFESMGIDTGIDLDGLLSTAQFCEQTLGRELYGRVTRSGLNPLI"
     gene            816..1073
                     /locus_tag="VFX94_13050"
     CDS             816..1073
                     /locus_tag="VFX94_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769154.1"
                     /translation="MRLASGHEALVARVLATDGKTGFGFSLRLDATEARHMAEWHAGA
                     RSERPKIEPLLAHPWETAYVAGEAVPWELEPAFAAVKWLSG"
     gene            1058..1516
                     /locus_tag="VFX94_13055"
     CDS             1058..1516
                     /locus_tag="VFX94_13055"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX5769155.1"
                     /translation="MAIRLENRIPPPIVAVGFAAGMLGVAWVAPALTFPLPSMLALPV
                     GAAGLLLDGIALFHFLRRRTTINPLKPQSASALVTGGIYRLSRNPMYLGLASLLLAWA
                     IYLGNLAALAGLPCFILYMNRFQIGPEERALQARFGAGYLAYRMRVRRWL"
     gene            1550..2245
                     /locus_tag="VFX94_13060"
     CDS             1550..2245
                     /locus_tag="VFX94_13060"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5769156.1"
                     /translation="MAQISYERPDGKTVNAYLAEPKAGKGAPGVVVIQEWWGLNDQIK
                     AVAEKLAAAGYRALVPDLYRGKVAVEAKEAEHLMTGLNFGDAAGQDVRGAVQHLKASG
                     SRKVGVTGFCMGGALTVLSAVNVPEADAAVAWYGFPPLEYVDATKIKAALMGHWATKD
                     EFFTISKVDELEKKLRDAKVKFEFHRYDAKHAFANETAVGPKRPVAAAEYNAQAAELA
                     WKRTLDFFAKNLK"
     gene            complement(2246..3196)
                     /locus_tag="VFX94_13065"
     CDS             complement(2246..3196)
                     /locus_tag="VFX94_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5769157.1"
                     /translation="MRKIVLLLLLLPALAAAQSYPSKPVRIVLPFGPGGIADITTRTI
                     APKLGEGLGQQVLVENMPGAGGIRAAETVARAEPDGHTLLLLTNGNAVSQALFKSLPY
                     DPVNDFAMISTVGFFSMVIVTRPESPAKTLQEAIAMAKANPGKMNIGTITPGGTQHLA
                     GELFRSTAGIDALVVPHKTTGEVIVAARNGNVDLAVDFIAPLLSSIKSGALRPLAVTA
                     AKRFPGLPEVPTVIEAGVAGYDVASWNAMAAPAKTPRAVIDRVNQELRKALSAEEVQR
                     RFGELGVEARASTPEELRKFFIAESQRWTRVVEAAKIPKQ"
BASE COUNT          561 a         1057 c         1137 g          561 t
ORIGIN      
        1 ccccgcgtcg aggccacgtc ctactccaac cccaaggtcg tgcctcagtt cgccgacgct
       61 tcggagctgt tgaaatccat tcagagaaaa tccggagttt cctacaaggc gacctgcgcc
      121 aacgtgagag cggtggagcg cgcgctcgcc gatctcgagg ccggccacgg cgcaagcgag
      181 gtgagcctgc tcgtgtcggc gaccgaatcg cacacgcgca agaacctgaa ccgcaaccgc
      241 gccgaacaat ggcagaacat cgccgagatg gtcgcggcgg ccaacgggcg cttgcgtatg
      301 gtagggacaa tttcggttgc gttcggctgc ccgttcgaag gcagcgtaga tccgggctcg
      361 gtgcttgcgg acgtggagag gtttgcccgg ctgggtgtgg ctcacgtggc gctgggcgat
      421 acgaccggca tggccacgcc gcctgccgta cgaaaaatct tcaagtcgtt tcccgggtca
      481 gtggtcggaa ttgcacacgt ccacgccacg cgcggcacgg ggctcgtgaa ctgcgtcgcc
      541 gcgtacgagg cgggcgtgcg ccacttcgat tcctccttcg gcggcgtcgg cgggcatccg
      601 gcgaaggtcc agtacggcgg cggcttcacc ggcaacgtgt gcaccgagga cctggtgaac
      661 ctgttcgagt cgatgggcat cgataccggc atcgacctcg acggcctgct ctccaccgcg
      721 cagttctgcg agcaaacgct cggccgcgag ctgtacggcc gcgtgacgcg cagcgggctg
      781 aacccattaa tctagctttc acccacgtcc attcggtgcg gctcgcctcg ggccatgaag
      841 cgctcgtcgc acgcgtgctt gccacggacg gcaagaccgg cttcggcttc agcctgcgcc
      901 tggacgccac cgaggcgagg catatggcgg aatggcatgc gggcgcgcgc agcgagcgcc
      961 cgaagatcga gccgctgctc gcgcatccgt gggagacggc ttacgtcgcg ggagaagcgg
     1021 ttccctggga gctcgagccg gcgtttgccg cggtcaagtg gctatccggc tagagaaccg
     1081 cataccgccg ccgatcgtcg cggtaggctt cgctgccggc atgctcggcg ttgcatgggt
     1141 cgcgcccgcg ctgaccttcc cgcttccttc aatgcttgcg ctgccggtcg gcgcggcggg
     1201 gctcctgctc gatggcatcg cgcttttcca ttttctgcgc aggcgcacga cgatcaatcc
     1261 gttgaaaccg cagtcggcgt cggcactcgt cacgggcggg atttaccggc tgagccgcaa
     1321 tccgatgtat cttggcctcg caagcctgct gctcgcatgg gcgatctacc tgggaaatct
     1381 cgccgccctg gcggggctgc cgtgcttcat cctatatatg aacaggttcc agattggccc
     1441 cgaagagcgg gcgctgcagg cgcggttcgg tgccggatac ctcgcctacc ggatgcgcgt
     1501 gcggcgctgg ctttgaagga aaatcaggtt ctgacccgag gaggcatgta tggctcaaat
     1561 cagctacgaa agacctgacg gaaagaccgt gaacgcctat ctggccgaac cgaaggccgg
     1621 aaagggcgcg cccggcgtgg tggtgatcca ggagtggtgg ggcctgaacg accagatcaa
     1681 ggccgtcgcg gagaagctcg ccgccgcggg ttatcgcgcg ctcgtgcccg atctctatcg
     1741 cggcaaggtc gccgtcgagg cgaaggaagc cgagcacctg atgaccgggc tcaacttcgg
     1801 cgacgccgcc gggcaggacg ttcgcggcgc ggtgcagcac ctcaaggcga gcggcagccg
     1861 caaggtcggc gtgaccggct tctgcatggg cggcgcgctt accgtcctca gcgccgtcaa
     1921 cgtccccgag gccgatgcgg cggtcgcgtg gtacggattt ccgccgctcg aatacgtcga
     1981 cgcgacgaag atcaaagccg cgctcatggg ccactgggcg acgaaggacg agtttttcac
     2041 catctcgaaa gtggacgagc tcgagaagaa gctgcgagac gccaaggtga agttcgagtt
     2101 ccatcgctac gacgcgaagc acgcgttcgc caacgagacg gcggttgggc ccaagcgtcc
     2161 ggtggcagcg gctgaataca acgcgcaggc ggccgagctc gcctggaagc gcacgctcga
     2221 cttcttcgcg aagaacttga agtaattact gcttcggaat cttcgccgcc tcgaccacgc
     2281 gcgtccagcg ctgggactcg gcgatgaaga acttgcgcag ctcctccggg gtgcttgcgc
     2341 gcgcctcgac gccgagctcg ccgaagcgcc gctgcacctc ctcggcggag agtgccttgc
     2401 gcagctcctg gttcacccgg tcgatgaccg cgcgcggcgt tttggccggc gccgccatcg
     2461 cgttccacga ggcgacgtcg tagccggcga cgccagcttc gatgacggtc ggcacctcgg
     2521 gcaggccggg gaagcgcttc gctgcggtga cggcaagcgg ccgcagcgca ccggacttga
     2581 tgctcgagag aagcggcgcg atgaaatcca cggcgagatc cacgttgccg ttgcgcgccg
     2641 cgacgatgac ttcaccggtg gtcttgtgcg gcacgacgag cgcgtcgatg ccggcggtgg
     2701 agcggaaaag ctcgcccgcg aggtgttgcg tgccgccggg ggtgatggtg ccgatattca
     2761 tctttcccgg gttcgccttg gccatggcga tcgcctcctg cagggtcttc gcgggtgatt
     2821 cgggccgggt gacgatcacc atgctgaaga agccgacggt cgagatcatg gcgaagtcgt
     2881 tcaccgggtc gtagggcagc gacttgaaga gcgcctggct caccgcattg ccgttcgtga
     2941 ggagcaacag cgtgtgcccg tccggctcgg cgcgcgccac cgtctccgcg gcgcggatgc
     3001 cgcccgcgcc gggcatgttc tcgacgagca cctgctggcc aagcccctcg ccgagcttag
     3061 gagcgatggt ccgcgtggtg atgtcggcga tgccccccgg gccgaacggc aggacgatgc
     3121 gcacgggttt cgagggatag gactgcgcag ccgcgagcgc cggcagcaag agcagaagga
     3181 gaacgatctt tctcattgga agagctcctc gaccgtgaac cgccgcggaa tcatattctg
     3241 cttataggcg caatcgatgg cgatatcgag gctgcgccgg ttagcctcga gcccgtgcgg
     3301 cagcatgggc gggtcc
//
LOCUS       DASUNT010000651         3953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_82328, whole genome shotgun sequence.
ACCESSION   DASUNT010000651 DASUNT010000000
VERSION     DASUNT010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82328"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2785)
                     /locus_tag="VFX94_13070"
     CDS             complement(<1..2785)
                     /locus_tag="VFX94_13070"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5769158.1"
                     /translation="MRLPELCIRRPVLATVLSLMIVLIGAISFSRLSVREYPKIDTPV
                     VSVRTVYKGASPTVMESQITQPLEDSIAGIEGVRTVKSVSREEVSQITVEFLPERDVD
                     SAANDVRDRVARVRAQLPEASDDSVVSKIEADAQAIIWMGLSSDKHSSLELSDYADRY
                     IADRLKTLPGVASVIIGGGRRYAMRIWLDRERLAAYSLTPQDVENALKRQNVEIPAGR
                     IESARREFTVLAEADLRTAEQFNNMIIVENRGYPVKLRDVGSARLGALDERSIIRRNG
                     QPGVGLGVVKQSTANTLSVAQAAKAEMARIQPTLPEGMQLQVSFDSSLFIERSIDSVY
                     RAMGEAVVLVMAVIFVFLRSLRATLIPFVTIPVSLVGSFFFLYLMGFTINVLTLLGVV
                     LAIGLVVDDAIVVLENCHRHIEMGKSRVQAAADGSGEIAFAVVAMSLTLTAVFAPVAF
                     VPGNTGKLFAEFALTVAAAVAVSGFVALTLTPMMCSKILKEHEKHGGLYLAMERFFHA
                     VTEGYRRAVGVTLRKPFIPLAAFAVVVAGTGFVYQKLRHELSPLEDRGYFLTLINAPE
                     GASMEYTDGFVRAVERMYADVPEIRSMFAVVAPGLERPNPVNFGVTFTQLAPWEERSR
                     KTQQITASMAPRLAASLPGVLAFPVNPNSLGQGFRTPQVQFVIQDNTYEDLDASVNKI
                     LEKTRTSGSIVNADSDLKLNKPQLSVDIDREKAAALGIDVEVIGRTLETLLGGRQVTR
                     FKREGKQYDVMVQLEAKDRSTPADLTSIYVRARDGTLVQLSNLVRVRETVAAKELNHF
                     NRARAAIISANVAPGSTLGEALDFLDGIAKELLPASARTDLDGVSREFRESGAGLAFV
                     ILFAALFIYLMLSAQFESFVSPFVIMLTVPLAGLGALAAIWLTGGTLNVYSKVGLVML
                     IGLITKHGILIVEF"
     gene            complement(2787..3839)
                     /locus_tag="VFX94_13075"
     CDS             complement(2787..3839)
                     /locus_tag="VFX94_13075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5769159.1"
                     /translation="MRLVAVALVLALLPAFAQQPGKPPAALPVKAVPAKLARAVIETG
                     AVGSLRADEAVVIRPEIAGRIDRIAFEEGQNVKKGALLASLDAAETRALVASSRAQAG
                     LDRQRMERAADLHKKGFISQQALDEMQSNYARSAAKLREDEAKLAKSEIRAPFSGVAG
                     LRQVSEGAYVAAGTDIARLEKIDQLKLDFRVPESYLAQLKTGLALSIAVDAFPQASFP
                     GQIYAIEPAVDEQTRTVLVRARVANPQLRLRPGLFARVQLTLGARENAVWIPEEAIVP
                     RGQESYVFRVVDSRAELVAVTTGTRKVGEVEIMKGIAAGDIVVTEGTQRLAPGMQVSI
                     IPSAKPAAAIPGKKGG"
BASE COUNT          651 a         1330 c         1346 g          626 t
ORIGIN      
        1 caaattccac gatcaggatg ccgtgcttgg tgatcaaccc gatcagcatt accagcccca
       61 ccttggaata gacgttgagc gtcccgccgg tgagccagat ggcggcgagc gcgccgaggc
      121 cggcgagcgg cacggtaagc atgatgacga agggagagac gaagctctcg aactgcgccg
      181 agagcatgag gtagatgaag agcgcggcaa agagaatgac gaaggcgagg cccgcaccgg
      241 actcgcggaa ctcgcgcgac accccgtcca agtcggtgcg cgcgctggcc gggaggagct
      301 ctttggcaat cccatccagg aagtcgagcg cttcgccgag cgtcgagccg ggcgcgacgt
      361 tcgccgagat gatcgccgcc cgtgcgcggt tgaagtggtt gagctccttc gccgccacgg
      421 tctcgcgcac gcgcaccagg ttcgagagct gcaccagcgt gccgtcgcgc gcgcgcacgt
      481 agatcgaggt caggtctgcg ggcgtgctgc ggtccttcgc ctcgagctgc accatcacgt
      541 cgtactgctt gccctcgcgc ttgaagcgcg tgacctggcg cccgccgagc agcgtctcga
      601 gcgtgcgccc gatcacctcg acgtcgatgc cgagggccgc ggccttctcg cggtcgatgt
      661 cgaccgagag ctgcggcttg ttgagcttca ggtccgagtc cgcgtttacg atcgacccgg
      721 aggtcctggt cttctccagg atcttgttga ccgaggcgtc gaggtcttcg taggtattgt
      781 cctggatcac gaattgcacc tggggcgtgc ggaaaccctg gccgagcgag ttcgggttca
      841 ccgggaaggc aagcacgccg ggcagcgacg ccgcgagccg cggcgccatc gaagcggtga
      901 tctgctgggt cttgcggctg cgctcttccc acggcgcaag ctgcgtaaac gtgacgccga
      961 agttcaccgg gttggggcgc tctagccccg gcgccaccac cgcgaacatg ctgcggattt
     1021 ccggcacgtc ggcatacatg cgctccaccg cgcgcacgaa gccgtcggtg tactccatcg
     1081 acgcgccctc gggcgcgttg atcagcgtga ggaagtagcc ccggtcctcg agcggcgaga
     1141 gctcgtgcct gagcttctgg tacacgaaac ccgtcccggc cacgaccacg gcgaacgctg
     1201 caaggggaat gaacggcttc ctcaaggtga cacccacggc gcgccgatag ccttcggtca
     1261 ctgcgtggaa gaagcgctcc atggcgaggt agaggccgcc gtgcttttcg tgctccttga
     1321 ggattttcga gcacatcatc ggcgtgagcg tcagcgcgac gaagcccgag accgcgaccg
     1381 ccgcggccac ggtgagagcg aactcggcga agagcttgcc ggtgttgccg ggaacgaagg
     1441 cgacgggggc gaacaccgcg gtaagcgtaa gcgacatcgc gacgaccgcg aacgcgatct
     1501 cgcccgagcc gtcggccgca gcctgcacgc ggctcttgcc catctcgatg tggcggtggc
     1561 agttctccag caccacgatc gcgtcgtcga ccacgaggcc gatggcaagc accacgccga
     1621 gcagcgtcag cacgttgatc gtgaacccca tcaggtacag gaaaaaaaac gagccgacga
     1681 gcgagaccgg gatggtgacg aaggggatca gcgtcgcgcg cagcgagcgc aggaagacga
     1741 agatcaccgc catcacgagc accaccgcct cgcccatcgc gcggtacacc gagtcgatcg
     1801 agcgctcgat gaaaagcgag gagtcgaagg agacctgcag ctgcatgccc tcgggcaggg
     1861 tgggctggat gcgcgccatt tccgcctttg ccgcctgcgc cacggagagc gtgttcgcgg
     1921 tggactgctt cacgacgccg agcccgacgc cgggctggcc gttgcgccgg atgatgctgc
     1981 gctcatcgag cgcgccgagc cgcgccgagc cgacgtcgcg cagcttcacc gggtagccgc
     2041 ggttctcgac gatgatcatg ttgttgaact gctcggcggt ccgcaggtcc gcttcggcga
     2101 gcacggtaaa ctcgcggcgc gcggactcga tgcgccccgc cgggatctcg acgttctggc
     2161 gcttgagcgc gttctccacg tcctgcggcg tgagcgagta ggcggcaagc cgctcccggt
     2221 cgagccagat gcgcatcgca tagcgccggc cgccgccgat gatcacggaa gcgacgccgg
     2281 gcagcgtctt caggcggtcg gcgatgtagc ggtcggcgta gtcggagagc tcgagcgacg
     2341 aatgcttgtc gctcgagagt cccatccaga tgatggcctg cgcgtcggcc tcgatcttgg
     2401 agaccaccga gtcgtcggag gcctcgggga gctgcgcgcg cacgcgcgcc acgcggtcgc
     2461 gcacgtcgtt cgccgccgag tcgacgtcgc gctcgggcag gaactcgact gtgatctggc
     2521 tcacttcctc gcgcgacacc gacttcaccg tgcgcacgcc ctcgatcccg gcgatcgagt
     2581 cctcgagcgg ctgcgtgatc tgcgactcca tcaccgtcgg actcgctcct ttgtagacgg
     2641 tgcgtaccga cacgaccggc gtgtcgatct tcggatactc gcgcaccgag aggcgcgaga
     2701 aggagatggc gccaatcagc acgatcatga ggctcagcac cgtggcgagc accgggcggc
     2761 ggatgcagag ctccggcagc cgcacgctag ccgcctttct tcccggggat ggcggccgca
     2821 ggcttggcgc tcgggataat cgagacctgc atcccgggcg caaggcgctg agtgccctcg
     2881 gtgacgacga tgtcgcccgc agcgatgccc ttcatgatct ccacctcgcc caccttgcgc
     2941 gtgccggtcg tcacggcaac gagctcggcc cggctgtcga ccacccggaa tacatagctc
     3001 tcctgaccgc gcgggacgat ggcctcctcc ggaatccaca ccgcgttctc gcgcgccccg
     3061 agcgtgagct gcacgcgcgc gaacaatccc gggcgcagcc tcagctgggg attggcgacc
     3121 cgcgcgcgca cgagcaccgt gcgcgtctgc tcgtcgacgg cgggctcgat cgcgtagatc
     3181 tggccgggaa agctcgcctg cggaaacgca tccaccgcga tgctcagcgc gaggccggtc
     3241 ttgagctggg cgagatagct ctccggcacg cggaagtcga gcttgagctg gtcgatcttc
     3301 tcgaggcgcg cgatgtcggt gccggcggcc acgtaggcgc cttcgctcac ctggcgaagt
     3361 cccgcgacgc cggagaacgg cgcgcggatc tcgctcttcg cgagcttcgc ctcgtcctcg
     3421 cgcagcttgg ccgccgagcg cgcgtagttc gattgcatct cgtcgagcgc ctgctggctg
     3481 atgaagccct ttttgtgcag gtcggcggcg cgctccatgc gctgcctgtc gagcccggcc
     3541 tgcgcgcgcg aagaggcgac gagcgcgcgc gtctccgcgg cgtcgaggct tgcgaggagc
     3601 gcccctttct tcacgttctg gccttcctcg aacgcgatgc ggtcgatccg cccggcaatc
     3661 tccggccgga tcacgacggc ctcgtcggcg cgcaggctgc ccaccgcgcc ggtctcgatc
     3721 accgcgcgcg caagcttggc cgggaccgcc ttgaccggaa gcgcggccgg aggttttccg
     3781 ggctgctggg cgaaggcagg aaggagcgct aaaacaagcg cgacggcgac aagacgcacc
     3841 gtgcggattc tactcggccc ccgagaaggc ccgggcgtct cgccgcgcta gcggcggtac
     3901 cactgccgca gctctatggg gttgccgtcc ggatccctga tctcggcgcg agc
//
LOCUS       DASUNT010000652         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_258531, whole genome shotgun sequence.
ACCESSION   DASUNT010000652 DASUNT010000000
VERSION     DASUNT010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258531"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1877)
                     /locus_tag="VFX94_13080"
     CDS             complement(<1..1877)
                     /locus_tag="VFX94_13080"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEX5769160.1"
                     /translation="MEDDGLFILSSYFLGVEHPPGYPLYTLLGWLFTFLPFGSVAYRV
                     HLLSGLFGALTCALVWLCARQLSERRLAAYMSALALGVTPVFWSQSLIAEVYTFNTFF
                     FALLTYLALQSSARLLPWMALVFGLSLSNHWPLMLLAVPAFAVLLWPLRSELARGSVA
                     LAALVLVGLLPYVWMVRRSWMDLPINFDGPLESLWEVWFFVSRSGYAQVDQSPSAGWL
                     DRVKFFRFQGEQLAYQFALAGTLLAATGFVAQWRLLPRRVCGFLTIAFLMPTAVLLML
                     LGFDYDTVTKHMYHVYPLPAYVVVALWLGLGSVWLAQRLRVRPQHAGAGGAAVLALIF
                     ALGSRSNMLGDYDWAARYAKATLEILPRGAIVFARSDADLSALAYFHLVEGVRPDIKL
                     YQPKGLVLGTRLFHPLRVTQKEMDTKLVEFIEQAPAPVVFTGEFYTGNARHDRWLHAE
                     VDRSSRDPYQATIEIPDQAVRFFEESILHVQERNAWIAYHQDELRRRYAILLAHRLKP
                     GQPPAERDARHLKAMSQDFFGALGLAEGLLGRGGGYSAGAVVQMLEQVRDLMPSDATK
                     AHRSKFFYLRGLVRLDLGDRSGGIQDLETALGVWPTRENPALQPLEDLWRASQPQAAE
                     RP"
     gene            complement(1973..>2360)
                     /locus_tag="VFX94_13085"
     CDS             complement(1973..>2360)
                     /locus_tag="VFX94_13085"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX5769161.1"
                     /translation="RFFLFAAVGVTGLGVHFGVLSAVFQAGAGFLASQALATWAAMTS
                     NFFLNNVFTYGDQRLRGRAVWRGLLSFYVACGLGAFINVAVAEWLYLQSAPYWAAGMA
                     GALLAAVWNFFTTASFTWGGARRRTR"
BASE COUNT          472 a          769 c          807 g          312 t
ORIGIN      
        1 gggcgctcgg cggcctgcgg ctggctggcg cgccacaggt cttcgagcgg ctgcagggcc
       61 gggttttccc gggtgggcca taccccgagc gcggtttcga ggtcctggat cccgccgcta
      121 cgatcgccca gatcgagccg caccaggccc cgcaggtaga agaattttga gcgatgcgcc
      181 ttggtggcgt cggagggcat cagatcgcga acctgctcga gcatctgcac gaccgcgccg
      241 gccgaatacc cgccgccgcg gccgagaagg ccttccgcca gcccgagcgc gccgaaaaag
      301 tcctgcgaca tcgctttcag atggcgcgca tcacgctcgg ccggcggctg gccgggcttc
      361 agccggtgcg cgagcaggat ggcgtagcgg cgccgcagtt cgtcctggtg ataggcgatc
      421 caggcgttgc gctcttgaac gtgcaggatc gactcttcaa aaaagcgcac cgcctggtcc
      481 gggatctcga tcgtcgcctg gtaggggtcg cgcgaggagc ggtccacctc ggcgtgcagc
      541 caccggtcgt gccgggcgtt cccggtatag aactcaccgg taaatacgac cggcgcgggc
      601 gcttgctcga tgaactccac cagcttggtg tccatttctt tctgcgtaac gcgcagcgga
      661 tggaacaagc gggtgccgag cacgagtccc ttaggctgat agagcttgat atcgggccgc
      721 acgccctcga cgagatggaa atacgcgagc gccgaaagat cggcgtcgct ccgcgcgaac
      781 acgattgcgc cgcgcggcag aatttccagc gtggccttcg cgtagcgtgc cgcccaatcg
      841 tagtcgccga gcatattgga gcggctgccg agagcgaaaa tgagcgcgag caccgctgcg
      901 ccgcccgccc cggcgtgctg cgggcggact cgcaggcgct gcgcgagcca gacgctgccg
      961 aggccgagcc aaagcgccac caccacgtac gcaggcaacg gatatacgtg gtacatgtgc
     1021 ttggtcacgg tgtcgtagtc gaatccgagc agcatgagaa gcactgcggt cggcatcagg
     1081 aaagcgatgg tcaggaatcc gcagacgcgg cgcgggagca gccgccactg cgcgacgaac
     1141 cccgttgctg caagcagcgt ccccgcgagc gcgaactggt acgcgagctg ttcgccttgg
     1201 aagcgaaaga acttcacccg gtcgagccac cccgctgacg gagactggtc gacctgggcg
     1261 tagccggagc ggctgacgaa gaaccacacc tcccacagcg actcgagggg cccgtcgaaa
     1321 ttgatcggca ggtccatcca agagcggcgc accatccaga catagggcag taggccgacg
     1381 agcaccagcg cggcgagcgc gacgctgccc cgcgccagct cgctgcgcaa cggccacagc
     1441 aacaccgcga aggccggcac ggcgagcagc atcaatggcc agtgattgct taggctcagg
     1501 ccgaagacga gcgccatcca cggcagcagg cgcgcgctcg actgaagcgc aagataggtg
     1561 agcagcgcga agaagaacgt attgaaggta taaacctcgg cgatcagcga ttgcgaccag
     1621 aacaccggcg taacgccgag cgcgagcgcg ctcatgtagg ccgccaggcg ccgctcgctg
     1681 agctggcgcg cgcaaagcca gaccagcgcg caggtcaggg cgccgaacag gccgctcagc
     1741 aggtgtacgc ggtacgcgac cgagccgaaa ggcaggaaag tgaacagcca gccgagcagc
     1801 gtgtacagcg ggtagccggg agggtgctcg actccgagga aatagctcga caggatgaaa
     1861 aggccgtcgt cctccaacgc gaccgtgcgc ggcgccgtgg ctgcgtacag cgctaagagc
     1921 gcaccggcga cgagtccggc ctgcgcccag tcggcgcgat tggaacgcgc gttcagcgtg
     1981 tcctccggcg cgcgccgccc caggtgaaag aagcggtggt gaagaaattc cagacggcgg
     2041 caagcagggc gccggccatc cctgcggccc agtagggcgc ggactgcagg tacagccact
     2101 ccgccaccgc gacgttgatg aacgcgccga ggccgcacgc gacatagaaa ctcagcaggc
     2161 cgcgccacac cgcgcgtccg cgcaagcgct gatcgccata ggtgaagacg ttgttcagga
     2221 agaagttgct cgtcatcgct gcccaggtgg cgagcgcctg gctcgcgagg aatccggcgc
     2281 cggcctggaa cactgcgctc aggaccccga aatgcacgcc gaggccggta acgccgacgg
     2341 ccgcgaagag aaagaaccgc
//
LOCUS       DASUNT010000653         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_696275, whole genome shotgun sequence.
ACCESSION   DASUNT010000653 DASUNT010000000
VERSION     DASUNT010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696275"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFX94_13090"
     CDS             complement(<1..192)
                     /locus_tag="VFX94_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5769162.1"
                     /translation="MGAAAGFAVFALVFGIVPFLGLAPFYETFLYLLLHAIVLATSWN
                     ILSGYSGYFSFGHGAFFGAG"
     gene            complement(192..1037)
                     /locus_tag="VFX94_13095"
     CDS             complement(192..1037)
                     /locus_tag="VFX94_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5769163.1"
                     /translation="MPSATLLGQSLLSGLFIGALYGLLGLGLSLSWGMLRQVNLAHFA
                     LAFLGGYLTLQLAGRFDPFVTLAVTVPLFFLAGMALHWFLSRYAPDMLNTLLVTFGLT
                     TIIEAGIQWIWTADFQRMESHYGAMRWKVGAFYLPLPELVTLVFAMLLCGSMWALLRF
                     TDFGRAARAMTEDAAMAAAFGVNEKRLGYVISGLAAAFAAVAGVCLALTHTLAPVQIY
                     AWMGVVFAGVMIGGLGSALGPLLAGFLIGVSEALTMAVTAPTWAPLVSFSLLIAIILF
                     RPARF"
     gene            complement(1055..>2222)
                     /locus_tag="VFX94_13100"
     CDS             complement(1055..>2222)
                     /locus_tag="VFX94_13100"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5769164.1"
                     /translation="GAALSTLALPAALRAQGKPIRIGSTLPLTGPLASLAVIHKVTAE
                     IYVENLNKRGGLLGRPVEWIVRDDQSKPELTRTIYEQLITADKVDLIQSPYATASILA
                     AMGVAQRYNKVLLHTSFGIPKLAKYEMQFQTAGVAFDPDNVWPNLIFDAAAKLPKPPK
                     TVAVVTSKFPSVHHISVGAREVMKKRGLQEVLHLEYEFGNRDFGPIASRVKDANPDFL
                     WGGTNGIDPVLMLEAMKRIDYRPPAQVHLFPAPGPMLKMAEAQGALALTNFEAHPPFT
                     DDPVIAQFVKTYAERSKEAGIPYPAVDLQSAIMYAAWQVLETVVTATKSLEDKAIAAW
                     LRNNEVKTLFGTLRWNGPQNYVEGSDLYKVKQLQQGRWIVIHPSQWAAPGATIV"
BASE COUNT          433 a          700 c          742 g          347 t
ORIGIN      
        1 gcctgccccg aagaaggcgc catggccgaa cgagaaatag ccggagtagc cgctgaggat
       61 gttccaggac gtggcgagca cgatcgcgtg cagcaagaga tacaggaacg tctcgtagaa
      121 cggcgcaagc ccgaggaagg ggacgatgcc gaagaccagg gcgaaaacgg cgaacccggc
      181 ggcagcgccc atcagaacct cgccggccgg aaaagaatga tggcgatgag aagcgagaac
      241 gacacgagcg gtgcccaggt cggcgccgtc accgccatgg tgagcgcctc gctcacgccg
      301 atcagaaatc ccgcgagcag cggcccgagc gcgctgccga gcccgccgat catgacccct
      361 gcaaagacca cccccatcca ggcgtagatc tgcaccggcg cgagcgtgtg cgtgagcgcg
      421 aggcatacgc cggcgaccgc ggcgaaggca gcagcgaggc ctgaaatcac gtagcccagc
      481 cgcttttcgt tgacgccgaa cgccgccgcc atcgctgcgt cttcggtcat tgcgcgcgcc
      541 gcgcgaccga aatcggtgaa gcgcagcagc gcccacatcg agccgcagag gagcatggcg
      601 aagacgagcg ttacgagctc cggcagcggc aggtaaaagg cgccgacttt ccagcgcatc
      661 gcgccatagt ggctctccat gcgctggaag tcggccgtcc agatccactg aatgcccgcc
      721 tcgatgatcg tcgtcaggcc gaaagtgacg agcagcgtgt tcagcatgtc gggcgcgtag
      781 cgcgacagga accagtgcag cgccatgccg gcaaggaaga acagcggcac ggtaacggcg
      841 agcgttacaa atggatcgaa gcgccccgca agctgcaagg tgagatagcc gccgagaaag
      901 gcaagcgcga aatgcgccag gtttacctga cgcagcatgc cccacgagag cgaaagacca
      961 agcccgagca gcccgtacag cgcgccaatg aagaggccgg acagtaaaga ctgcccgagg
     1021 agtgtcgcgc tcggcaccta gcttccggcg aggctcagac gatagtcgcg ccgggcgcgg
     1081 cccactggct ggggtggatc acgatccacc tgccctgctg gagctgcttt accttgtaca
     1141 gatccgagcc ttcgacgtag ttctgcggcc cgttccagcg cagggtgccg aagagcgtct
     1201 tcacttcgtt gttcctcagc cacgcggcga tcgccttgtc ctccaggctc ttcgtcgccg
     1261 tgaccaccgt ctcgagcacc tgccaggcgg catacatgat cgcggactgc agatcgaccg
     1321 cgggataggg aatgccggct tccttgctgc gctcggcgta ggtcttcacg aactgggcga
     1381 ttacggggtc atcggtgaac ggcggatgcg cctcgaaatt ggtgagcgcc aacgcgccct
     1441 gtgcctccgc catcttcagc atcgggccgg gcgcggggaa gagatgcacc tgcgccggcg
     1501 gccggtagtc gatgcgtttc atcgcctcga gcatgagcac gggatcgatg ccattggtgc
     1561 cgccccacag gaagtcgggg ttcgcgtcct tgacgcggct cgcgatcggg ccgaagtccc
     1621 ggttgccgaa ctcgtactcg aggtgcagca cttcctgcag gccgcgcttc ttcatgactt
     1681 cgcgcgcgcc gaccgagatg tggtgcaccg agggaaactt gctcgtgacg acggccaccg
     1741 ttttcggcgg cttgggcagc ttcgctgcgg cgtcgaaaat gagattcggc cagacgttgt
     1801 cgggatcgaa ggcaactccg gcggtctgga attgcatctc gtacttggcg agcttgggaa
     1861 tgccgaacga ggtgtgcagc agcaccttgt tgtagcgctg cgccacgccc atcgccgcga
     1921 ggatcgaggc cgtggcgtaa gggctctgga tgagatcgac cttgtcggcg gtgatgagct
     1981 gctcgtagat cgtgcgcgtg agctcgggct tggactgatc gtcgcgcacg atccactcca
     2041 ccggccggcc gagcagcccg ccgcgcttgt tgaggttctc cacgtagatc tcggccgtca
     2101 ccttgtgaat cacggcgagc gaggcgagcg gtcccgtgag cggcagcgtg ctaccgatgc
     2161 gaatcggctt gccctgcgcg cgcagcgccg cgggcaatgc cagagtggac aaagctgcac
     2221 ct
//
LOCUS       DASUNT010000654         3972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_871315, whole genome shotgun sequence.
ACCESSION   DASUNT010000654 DASUNT010000000
VERSION     DASUNT010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871315"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VFX94_13105"
     CDS             complement(<1..940)
                     /locus_tag="VFX94_13105"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5769165.1"
                     /translation="MLTRRRFLGTLGAAALAGCGRPAPRGGDTTLVFKHGKLFGDPHV
                     LRGLLDEFERAHPGLRIREETLPSSSDEQYQFYAINLQARADVFDVLAVDIIWVAGFA
                     RAGWLKPLAAEGGFFRGPMEAVTWRGSVYALPWFVDAGLLYYRRDLVEKPPEHWHELP
                     QLARAPRLHGFVWQGKQYEGLVCNALEYLHSNGGAVLEGERAVLASPQNREALAFMAD
                     LVRRYGVTPASVTTLTEEPSRHVFGQGRAAFLRNWPYAWRLFQDPASRVHGKVAVAEL
                     PRFPGGRSAPTLGGWQLAVNVASRDPEAAEGLVRFMT"
     gene            complement(1173..3290)
                     /gene="glgX"
                     /locus_tag="VFX94_13110"
     CDS             complement(1173..3290)
                     /gene="glgX"
                     /locus_tag="VFX94_13110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX5769166.1"
                     /translation="MQSLRVWPGKPYPLGATWDGSGTNCALFSAHADKVELCLFDETG
                     EKETARIALPEFTHEIWHGYLPDVRPRQRYGYRVHGPYDPAAGHRFNPHKLLIDPYAK
                     ALAGALVWHDALFGYRVGDEKGDLSFDERDSAAYLPKCVVIDPAFTWGRERPAQRPWH
                     EAVIYEMHVRGFTMRRGDVPQEARGTFAGLSAAPVVQYLKELGVTAVELMPVHAFVHD
                     RHFVERGLRNYWGYNTLGFFAPHPEYLLHNSTSEVKTLVQLMHGAGIEVILDVVYNHT
                     AEGSHLGPTLSLRGIDNKTYYYLADDPRYYNDFTGTGNALELRHPYVLRMVTDSLRYW
                     VQEMRVDGFRFDLATTLARVNGKFDEHASFLDAVAQDPVLAGVKLIAEPWDTGPGGYQ
                     VGNFPPGWAEWNDQYRDTVRRFWKGDPGQLPEFASRISGSADVYHRRGRHTWASVNFV
                     TAHDGFTLRDLVSYDAKHNEANREDNRDGAEANHSWNCGAEGETSDAAILALRRRQMR
                     NFLATLLLSQGVPMLLAGDAIARTQGGNNNAYCQDNETSWIDWSLDAQAQSQLEYTRR
                     LVALRASHIVFHRSRHFEGTHIPGTEVKDVTWLRPDGAEMAEADWKNAEARTLGLLLS
                     GEAGLMHLTEAGEQETDDTFCLLFNAAHEDVAFTLPTPDGGRWQALIDTADEQGFVEG
                     PSFARGDRITLKARSLQLLRLVH"
     gene            complement(3431..>3972)
                     /locus_tag="VFX94_13115"
     CDS             complement(3431..>3972)
                     /locus_tag="VFX94_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiB"
                     /protein_id="HEX5769167.1"
                     /translation="MGSMLADMTTLLREHRLALPPDLALVLKACVTLETYGRELDPEF
                     DMVGEAAPFLEAATLERFGPARLARRGWRASRILLDTLADLPRDLRELVRSARSGRLQ
                     MHVDLTSLYRFGQQLDGAANRLAIGIVTAAFIVGSAIVMTVEGGPTLLGLPLFGLLGF
                     LGAALAGAWLLVSILRSGR"
BASE COUNT          620 a         1342 c         1412 g          598 t
ORIGIN      
        1 cggtcatgaa ccgcaccaag ccctcggcgg cctcggggtc gcgcgaggcg acgttcaccg
       61 cgagctgcca gccgccgagc gtgggcgccg agcgcccgcc cgggaaacgc ggcagctcgg
      121 cgacggccac cttgccgtgc acgcgcgagg cgggatcctg gaacaggcgc caggcataag
      181 gccagttgcg caggaacgcc gcgcggccct ggccgaagac atgccggctc ggctcttcgg
      241 tcagcgtggt gaccgaggcg ggcgtgacgc cgtagcgccg cacgaggtcg gccatgaagg
      301 cgagcgcctc ccggttttgc gggctcgcga gcaccgcgcg ctcgccctcg agcaccgcgc
      361 cgccgttgct gtgcaagtat tcgagcgcat tgcacaccag accctcgtac tgcttgccct
      421 gccacacgaa cccgtgcagc cgaggcgcgc gcgccagctg cgggagctcg tgccagtgct
      481 cgggcggctt ctccacgagg tcccggcggt agtagagcag gcccgcgtcg acgaaccagg
      541 ggagcgcgta cacgctcccg cgccaggtca ccgcttccat cggcccgcgg aagaagccgc
      601 cctcggcggc gagcggcttg agccacccgg cgcgcgcgaa gcccgccacc cagatgatgt
      661 ccactgccag cacgtcgaag acgtcggcgc gcgcctgcag gttgatggcg tagaactggt
      721 actgctcgtc gctcgacgaa ggcagggtct cctcgcgaat gcgcagcccc ggatgcgcgc
      781 gctcgaactc gtcgagcagg ccgcgcagca cgtgcgggtc gccgaagagc ttgccgtgct
      841 tgaagaccag cgtggtgtcc ccgccgcgcg gcgccggccg gccgcagccg gcgagcgccg
      901 cggcgcccag cgttcccaaa aagcggcgtc gcgtgagcat cgcccgccta ctctagacca
      961 tcgaaccggg agacgaactc caactgggga acgatcctct cgctccccga ctacccgcac
     1021 cacacgatcc gcctcgacgt gccgcaaggg gaaatcacca tcggcatctt cgccttccgc
     1081 ggcgcggacg aagcccgagc gggaagcacc gtcgccggcg agaacctcgc ggccggcagc
     1141 gggcaggcga ggtgatagaa gcgccagggc agctagtgaa cgagccgcaa gagctgcagc
     1201 gagcgcgcct tgagcgtgat cctgtcgccg cgcgcgaagc tcggcccctc gacgaagccc
     1261 tgctcgtcgg cggtgtcgat cagcgcctgc cagcggccgc cgtccggggt gggcagggtg
     1321 aaggccacat cctcgtgcgc ggcgttgaag agcaggcaga aggtgtcgtc ggtctcctgc
     1381 tcgccggcct cggtgaggtg cataagcccc gcttcgcccg acaggagtag cccgagcgtg
     1441 cgcgcctcgg cgttcttcca gtccgcctcg gccatctcgg cgccgtccgg gcgcagccac
     1501 gtcacgtcct ttacctcggt gcccgggatg tgcgtacctt caaagtgccg cgagcggtgg
     1561 aacacgatgt ggctcgcgcg cagcgcgacc agccggcgcg tgtactcgag ctggctttgc
     1621 gcttgggcgt cgagcgacca gtcgatccag ctggtctcgt tgtcctggca ataggcgttg
     1681 ttgttgccgc cttgggtgcg ggcgatggcg tcgccggcga gcagcatcgg caccccctgc
     1741 gaaaggagca gcgtggcgag gaagttgcgc atctggcggc ggcgcagggc gaggatcgcg
     1801 gcgtcgctgg tctcgccctc ggcgccgcag ttccagctgt gattggcctc cgcgccgtcg
     1861 cggttgtcct cgcggttcgc ctcgttgtgc ttggcgtcgt agctcaccag gtcgcgcagc
     1921 gtgaagccgt cgtgcgcggt gacgaagttg acgctcgccc aggtgtgccg gccgcggcgg
     1981 tggtagacat ccgccgagcc ggagatacgc gaggcgaatt ccgggagctg acccggatcg
     2041 cccttccaga agcgacgcac cgtgtcgcgg tactggtcgt tccactccgc ccaaccgggc
     2101 gggaaattgc cgacctggta gccgcccggc ccggtgtccc agggctcggc gatgagcttc
     2161 accccggcga ggacggggtc ctgggcgacc gcgtcgagga agcttgcatg ctcgtcgaat
     2221 tttccgttga cgcgtgcgag cgtggtagcg aggtcgaagc gaaagccgtc cacgcgcatc
     2281 tcctgcaccc agtagcgcag agagtcggtc accatgcgca gcacgtacgg gtggcgcaac
     2341 tccagcgcat tgccagtgcc ggtgaagtcg ttgtagtagc gcggatcgtc ggcgaggtaa
     2401 taataggttt tgttgtcgat gccgcgcagg gagagcgtcg gcccgaggtg gctgccctcg
     2461 gccgtatggt tgtagaccac gtcgagaatc acctcgatgc ccgcgccgtg catgagctgc
     2521 actaaggtct tcacttcgct cgtgctgttg tgcagcaggt actcgggatg cggcgcgaag
     2581 aagccgaggg tgttgtagcc ccaatagttg cgcagcccgc gctcgacaaa gtggcggtcg
     2641 tgcacgaacg cgtgcacggg catgagctcg accgcggtga cgccgagctc cttcaggtac
     2701 tgcacgaccg gggcggccga gagcccggcg aaggtgccgc gcgcctcctg cggcacgtcg
     2761 ccgcggcgca tggtgaagcc gcgcacgtgc atctcgtaga tcaccgcctc gtgccaaggg
     2821 cgctgcgccg ggcgttcgcg gccccaggtg aacgccgggt cgatcaccac gcacttgggc
     2881 aggtaagcgg cgctgtcgcg ctcgtcgaac gagagatcgc ccttctcgtc gccgacccgg
     2941 tagccgaaga gcgcgtcgtg ccagacgagc gcgcccgcga gcgccttggc gtagggatcg
     3001 atcagcagct tgtgcgggtt gaagcggtgg ccggcagccg ggtcgtaggg tccgtggacg
     3061 cggtagccgt agcgctggcg tgggcgcacg tcgggcagat agccgtgcca gatctcgtgc
     3121 gtgaactccg gcaacgcgat gcgcgcggtc tccttctcgc ccgtctcgtc gaaaaggcag
     3181 agctccacct tgtcggcgtg tgccgagaag agcgcgcagt tcgtgccgct gccgtcccag
     3241 gtcgcgccca gcggataggg cttgcccggc cacacgcgca atgactgcat cggccgctag
     3301 cttacgtgtg tagccgcttg tgcgcttcca tggcgaggag caggaccagg gccagggcgc
     3361 ctttcgagct tctagcatcg cagcttcggc gggaggacac ctgatctccc tcaaacaccc
     3421 tgatgacggc ctagcggccg ctgcgcagga tcgagacgag gagccaggcg ccggcgagcg
     3481 ccgcgcccag gaatcccagc aatccgaaga gcggcaggcc gaggagcgtg gggccgccct
     3541 cgaccgtcat cacgatcgcc gagccgacga tgaaggctgc ggtgacaatg ccgatcgcga
     3601 ggcgattcgc cgcgccgtcg agttgctggc cgaagcggta gaggctcgtg agatcgacgt
     3661 gcatttgcaa ccgcccgctg cgcgctgagc gcacgagctc gcgcaggtcg cgcggcaggt
     3721 cggcgagcgt gtcgagcagg atgcggctcg cgcgccagcc gcgtcttgcc aaccgcgcag
     3781 ggccgaagcg ctccagcgtg gcggcctcga ggaacggcgc cgcctcgccc accatgtcga
     3841 actccgggtc gagctcgcgg ccgtaggtct ccagggtgac gcaggctttc agcaccagcg
     3901 ctagatcggg cggcagcgcg agccggtgct cgcgcaagag cgtggtcatg tcggcgagca
     3961 tcgagcccat ac
//
LOCUS       DASUNT010000655         4443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1046453, whole genome shotgun sequence.
ACCESSION   DASUNT010000655 DASUNT010000000
VERSION     DASUNT010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046453"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VFX94_13120"
     CDS             complement(<1..319)
                     /locus_tag="VFX94_13120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5769168.1"
                     /translation="MPPPPIAMREQLREERFNGVWTQELNRVFADVAPYYDQANVVAS
                     LGMWEFLLRGFMDTMQLRAGERVLDVCAGTNAVGIALLKREPGLEVHAMDRSAEMQQV
                     GRRR"
     gene            complement(333..659)
                     /gene="frdD"
                     /locus_tag="VFX94_13125"
     CDS             complement(333..659)
                     /gene="frdD"
                     /locus_tag="VFX94_13125"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF003977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HEX5769169.1"
                     /translation="MARSNKPIVWGPFAAGGTLTAFLTPVLVLVTLLQPSGLSYESMH
                     ALASHWLAKIVFVGVIFFSLWSAAHRLRITLYDLGLRADKLVATVLYAATLACAGAAV
                     YYLLKL"
     gene            complement(659..1042)
                     /locus_tag="VFX94_13130"
     CDS             complement(659..1042)
                     /locus_tag="VFX94_13130"
                     /inference="COORDINATES: protein motif:HMM:NF014366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit C"
                     /protein_id="HEX5769170.1"
                     /translation="MSRQPYVRPMSSEWIFRHPRYVRYMVREFSCLFIGGWTALMVYG
                     LKQLSDGPAAWAAFLSLLKSPAGVVFQLLTLAFATYHSITWFNLTPKALPLQFGEDFV
                     PDWVIAAAHFAAWGLVSVVILALSL"
     gene            complement(1039..1773)
                     /locus_tag="VFX94_13135"
     CDS             complement(1039..1773)
                     /locus_tag="VFX94_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX5769171.1"
                     /translation="MRQVELEVFRYNPESDREPRFQRYTVECQEEWVVLDALNHVKET
                     LDPTLSYRWSCHMAVCGSCGMMINSEPKLACKAFLRDYEGTIRVEPLRHFPVERDLVT
                     VIDDFVAKLARVKPWLMPKEPPGTGEYRQTPAQLKRYKQFTLCINCMLCYAACPEYGL
                     IPGYIGPAAIALAHRWNRDSRDGGRDARQEEIASKEGAWDCSFVGACSEVCPEHVDPA
                     AAIQQVKIESTVDWYQAHLMPWLNDK"
     gene            complement(1770..3521)
                     /gene="frdA"
                     /locus_tag="VFX94_13140"
     CDS             complement(1770..3521)
                     /gene="frdA"
                     /locus_tag="VFX94_13140"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HEX5769172.1"
                     /translation="MESVAADVAIIGAGGAGLRAAIALAEAAPKLRIALVSKVYPMRS
                     HTCAAEGGAAGVIKPDDSLQQHFDDTVGGGDWLSDQDAVEYFIAEAPKELLQLERWGC
                     PWSREPDGSVAVRPFGGMKLMRTWFAADKSGFHILHTLFQTSLKYPSIQRFDEFYATD
                     LLVEDGRCRGLTAIEMRSGQMRRFDAAAVILAGGGAGRMFPFTTNGAIKTGDAMALAY
                     RAGVPLKDMEFVQYHPTGLPSTGTLLTEACRGEGGVLLNKHGRRFLQDYGMGPETPLG
                     KPVLKTMELGPRDRVSQAFWHEQKKGNAVSTRWGDAMLLDMRHLGEKKINERLPLVRD
                     MSISYMGVDPVTDPVPVRPVVHYMMGGIHTDIRAATPVAGLFAAGECACVSINGANRL
                     GSNSLTELLVFGRRAALSALDFFEKNSNPAPSAGLADKAQARIRALFQRTGSGQTVAG
                     LRKEMLHTMEEHAGIYRSGEGLAAACAKLAELRQRYRDVELHDKTNVYNTDLLQTLEL
                     GAMLECAEAVVQSAAARKESRGAHQRLDFPERDDKKYLRHSLAFYRGAEPPGVDYCGV
                     TITKSQPGVRDYSGAHV"
     gene            3645..>4443
                     /locus_tag="VFX94_13145"
     CDS             3645..>4443
                     /locus_tag="VFX94_13145"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX5769173.1"
                     /translation="MKQALIAAAAAFALAVPTAHAQQKVRLNLASAFPTSLTLIGEAP
                     VKLSQKLNRLSGGTLELRVNEPGALVPALQAIQATGQGSVDAAWSNAGFFAATDTAFN
                     MFAAVPFGPGIPEYLAWIHHGGGLQLSREMFGKHGVYNIPCAIIPPEASGWFRREIKS
                     VEDLKGLKMRFFGLGARAMNKLGVATQLLAPGDIFQALQLGTIDATEFSLPSMDQKFG
                     FYQVAKFYYFPGWHQQATFLEFYINASKWKSLSEQHRAMVESACGDLT"
BASE COUNT          731 a         1486 c         1478 g          748 t
ORIGIN      
        1 cgcgacgccg gcctacctgc tgcatctccg cgctgcgatc catggcgtgc acctcgaggc
       61 caggctcgcg cttgagaagc gcgatgccga cggcgttcgt gccggcgcag acgtcgagca
      121 cgcgctcgcc cgcgcgcagc tgcatggtat ccatgaagcc gcgcagcaga aactcccaca
      181 tcccgaggct cgcaacgacg ttggcctggt cgtagtacgg cgcgacgtcg gcgaacacgc
      241 ggttcagctc ctgcgtccag accccattga agcgctcctc gcggagctgc tcgcgcatgg
      301 cgatgggcgg cggcggcatg gtcccctcct ctttatagtt tgagcagata gtaaactgcg
      361 gccccggcgc aggcgagcgt ggcggcgtac agcaccgtgg cgaccagctt gtcggcgcgc
      421 agccccaggt cgtagagcgt gatgcgcagg cggtgcgccg cgctccaaag cgagaagaaa
      481 atgactccga cgaacacgat cttggcgagc cagtgggacg ccagggcgtg catcgactcg
      541 taggaaagcc cgctcggctg gaggagcgtc acgagcacga gcaccggcgt aagaaacgcg
      601 gtcagcgttc cgccggcggc gaagggcccc cagacgatcg gcttgttgga cctggccatc
      661 aaagggaaag cgcgaggatc accaccgaca cgagccccca tgccgcgaaa tgggctgcgg
      721 cgataaccca atccggcacg aagtcctcgc cgaactgcag cggcagcgcc ttcggcgtga
      781 gattgaacca ggtgatgctg tgataggtcg cgaacgcgag cgtcagaagc tggaatacga
      841 cgccggccgg gctcttcagc agcgagagga acgccgccca ggccgccggc ccgtcggaaa
      901 gctgcttcag cccgtacacc atcagggcgg tccagccgcc gatgaagagg cagctgaact
      961 cgcgcaccat gtagcgcacg tagcgggggt ggcgaaagat ccactcggaa ctcatcgggc
     1021 gaacgtaggg ctggcggctc atttgtcgtt cagccacggc atgagatgcg cctggtacca
     1081 gtcgacggtg ctttcgatct tgacctgctg gatcgctgcc gccggatcga cgtgctcggg
     1141 gcacacttcg gagcacgcgc cgacgaacga gcagtcccac gcgccttcct tggacgcgat
     1201 ctcttcctgg cgcgcatcgc ggccgccgtc gcgcgagtcg cggttccagc ggtgcgcgag
     1261 cgcgatggcc gccggaccga tgtagccggg aatcaggccg tattccgggc acgccgcata
     1321 gcaaagcatg cagttgatgc aaagcgtgaa ctgcttgtag cgcttgagct gcgcgggagt
     1381 ctggcggtat tcgccggttc cggggggctc cttcggcatg agccagggct tgacgcgcgc
     1441 gagcttggcg acgaagtcgt cgatcaccgt gacgaggtcg cgctcgaccg ggaagtggcg
     1501 cagcggctcg actcgtatcg tgccttcgta atcgcgcagg aaggccttgc aggcgagctt
     1561 cggctcgctg ttgatcatca tcccgcagct tccgcatacc gccatgtggc acgaccagcg
     1621 gtagctgagc gtcgggtcga gcgtctcctt cacatggttg agcgcgtcga gtaccaccca
     1681 ttcttcctgg cactcgaccg tgtagcgctg aaaccgcggc tcgcggtccg attcagggtt
     1741 gtagcggaat acctcgagct cgacctgtct cacacgtgcg ctcccgagta gtcgcgcacg
     1801 ccaggctgcg acttcgtgat cgtcacgccg cagtagtcga cgcccggggg ctcggcgccg
     1861 cgatagaagg cgagcgaatg gcgcaggtac tttttgtcgt cgcgctccgg aaaatcgagc
     1921 cgctggtgcg cgccgcgcga ctccttgcgc gcagccgcgc tctgcaccac ggcctcggcg
     1981 cactccagca tcgcgccgag ctcgagcgtc tgcagcagat ccgtgttgta gacatttgtc
     2041 ttgtcgtgca gctcgacatc gcggtagcgc tgccgcagct cggcgagctt cgcgcaggcg
     2101 gcggcaaggc cctcgccgct cctgtagatg cccgcgtgct cttccatcgt gtgcagcatc
     2161 tccttgcgca gcccggcgac ggtctggccg ctgcccgtgc gctggaaaag ggcccggata
     2221 cgggcttgcg ccttgtccgc caaaccggcg cttggcgcag gattgctgtt tttctcgaaa
     2281 aagtccaaag cgcttagcgc tgcgcgacgg ccgaagacca gcaactcggt gagcgaattg
     2341 gagccgaggc ggttcgcgcc gttgatgctt acgcaggcgc attcgcccgc cgcgaacagc
     2401 ccggcgaccg gggtcgccgc cctgatgtcg gtatggatgc cgcccatcat gtaatggacc
     2461 accgggcgca cgggcacggg atcggtgacc gggtcgacgc ccatgtagct gatcgacatg
     2521 tcgcgcacga ggggcagccg ctcgttgatc ttcttttcgc cgaggtggcg catgtcgagg
     2581 agcatcgcgt cgccccagcg ggtgctcacc gcgttgccct tcttctgctc gtgccagaag
     2641 gcctggctca cgcggtcgcg cggcccgagc tccatcgtct tgaggaccgg cttgccgagc
     2701 ggcgtctccg gccccatgcc gtagtcctgc aggaagcggc ggccgtgctt gttgagaagt
     2761 acgccgcctt cgccccggca ggcttcggtg agaagcgtgc cggtgctcgg caggccggtc
     2821 gggtggtact gcacgaactc catgtccttg agcggcacgc ccgcgcggta ggcaagcgcc
     2881 atagcatcgc cggtcttgat cgcgccgttc gtggtgaagg ggaacatccg cccggcgccg
     2941 cccccggcca agatcaccgc cgcggcgtcg aagcgccgca tctgcccgct cctcatctcg
     3001 atcgcggtca ggccgcggca gcgcccgtcc tcgaccagca ggtcggttgc gtagaactcg
     3061 tcgaagcgct ggatcgaggg gtacttgagc gaggtctgga agagcgtgtg caggatgtgg
     3121 aagcccgact tgtcggcggc gaaccaggtg cgcatgagct tcatgccgcc gaaggggcgc
     3181 accgccaccg agccgtcggg ctcgcggctc cacgggcagc cccagcgctc gagttgcagg
     3241 agctccttgg gcgcctcggc gatgaagtac tcgaccgcgt cctggtccga gagccagtcg
     3301 ccgccgccca ccgtgtcgtc gaagtgctgc tggaggctgt cgtccggctt gatcaccccg
     3361 gcggcgccgc cctccgcggc gcaggtgtgg ctgcgcatcg gatagacctt ggacacgagc
     3421 gcgatgcgca gcttcggggc cgcctctgcg agcgcgatcg ccgcccgcaa cccggcccct
     3481 cccgcgccga tgatcgctac gtcagccgct acgctctcca ccgccctatt tctccttcgc
     3541 ttgacgcgaa tgtgcaacga agccggcgtc ttcgagttgt cctagatcaa atccggcccg
     3601 attgtggata atgcaggcgc aaccataccc gagggggaga agacatgaag caggctctga
     3661 tcgccgcggc cgcagcgttc gcgctcgccg tgccgacggc tcacgcacag cagaaggtcc
     3721 gcctgaacct cgccagcgcg tttccgacca gccttacgct gatcggcgag gcgccggtga
     3781 agctctcgca gaaactcaat cgcctgtcag gcgggacgct ggagctgcgg gtgaacgagc
     3841 ccggcgccct cgtgcctgca ctgcaggcga tccaggcgac cgggcagggt tcggtcgacg
     3901 ccgcgtggtc gaacgcggga tttttcgccg ccaccgacac cgccttcaac atgttcgccg
     3961 cggtcccctt cggccccggg atcccggaat acctcgcctg gatccaccat ggcgggggcc
     4021 tgcagctctc gcgcgagatg ttcggcaagc atggcgtcta caacatcccg tgcgccatca
     4081 tcccgcccga ggcctcgggc tggttccggc gcgagatcaa gtcggtcgag gacctgaagg
     4141 ggctgaagat gcgctttttc gggctgggcg cgcgcgccat gaacaagctc ggcgtggcca
     4201 cccagctcct cgcgccgggc gatatcttcc aggcgctgca gcttggcacc atcgacgcga
     4261 ccgagttctc gctgccgtcg atggaccaga agttcggctt ctaccaggtg gcgaagttct
     4321 actacttccc cggctggcac cagcaggcga cgttcctcga gttctacatc aacgcgagca
     4381 aatggaagtc gctctccgag cagcaccggg cgatggtcga gtcggcgtgc ggcgacctca
     4441 cca
//
LOCUS       DASUNT010000656         2116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1311078, whole genome shotgun sequence.
ACCESSION   DASUNT010000656 DASUNT010000000
VERSION     DASUNT010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311078"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..533)
                     /locus_tag="VFX94_13150"
     CDS             complement(69..533)
                     /locus_tag="VFX94_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5769174.1"
                     /translation="MTTFRCARHDDLPAIVRLLADDPLGAKRERYETPLPQAYVRAFE
                     AIDADPNNELVVACLEGEVVGVLQVTFIPYLTYQGGWRALIEAVRIDSRQRSRGLGRK
                     MFEWAIARSRDRGCHLVQLTTDKSRPDAKRFYESLGFAASHEGMKLQLKSNP"
     gene            complement(530..>2116)
                     /locus_tag="VFX94_13155"
     CDS             complement(530..>2116)
                     /locus_tag="VFX94_13155"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5769175.1"
                     /translation="KHHTSKTMQAYLRLVQWGLAHRKLALLSGGVLLAASAAMGPFLA
                     KTFIPASDRGQTTINFELAPGTPLEETVAAAEAMRRIIAQHKEVTGVLAVIGAGAQLD
                     AIGTSSTGEVRAGTLTVKLLPENQRKLSQRQFERLIAPELAAVPGLRVRFGGGEAGET
                     FQLVLVSDSPAVLDRTAEAVERAMRSIPGVGNPAATSGLRRPEIAIRPDTSRAADLGV
                     TSADIATTARVGTIGDTSTLLPKFNLEDRSIPIRTQISREARGDLDELRLLRVPTAGG
                     GSVPLDNVAAVEMGSGPAQITRFDRRRSVTIKADIAGVPLGTVTEQIQALPSIKNLPA
                     EVKQTPYGDSERMNELFGEFAIAIVAGVLLVYCVLVLLFHDFAQPLTIMAALPLSVGG
                     ALGLLLASGEALSLPAIIGILMLMGIVTKNSILLVEYALVEIDKGVPRAEALIDACSK
                     RAQPIIMTTVAMGAGMLPIALKIGADADFRAPMAIAVIGGLITSTLLSLFYVPLVFTY
                     VDDIKHWLARSGGFLVLQKS"
BASE COUNT          331 a          668 c          779 g          338 t
ORIGIN      
        1 ggtgaggtgg gcgtagcgga tcggcggtgc ctgccacagg ggtacggcca tgacgaacgc
       61 gagcccgctc atggattgct cttcagttgc agtttcatgc cttcgtgcga ggcggcgaag
      121 ccgaggcttt cgtagaagcg cttcgcgtcc gggcgggact tgtcggtggt gagctgcacg
      181 aggtggcagc cgcggtcgcg gctgcgcgcg atcgcccatt cgaacatttt cctgccgagg
      241 ccccgggaac gttggcgcga atcgatccgc accgcctcga tcagtgcccg ccacccgccc
      301 tggtacgtga ggtaggggat gaacgtgacc tgcagcacgc cgaccacttc gccttcgagg
      361 caggcgacga cgagctcatt gttcggatcg gcatcgatcg cttcgaaggc gcgcacgtag
      421 gcctgcggga gcggcgtctc gtagcgctcg cgcttcgcgc ccagcgggtc gtcggcgagc
      481 aggcggacga tcgccggaag gtcgtcgtgc cgggcgcagc ggaaagtcgt catgactttt
      541 gcagaaccag aaatccgccg gaacgcgcga gccagtgctt gatgtcgtcc acgtaggtga
      601 acaccagcgg cacgtagaag agcgagagca gcgtcgaggt gatgaggccg ccgatcaccg
      661 cgatcgccat cggcgcgcgg aagtcggcat ccgccccgat cttgagcgcg atcggcagca
      721 tgccggcgcc catcgcgacg gtggtcatga tgatcggctg ggcgcgcttg ctgcacgcgt
      781 cgatcagcgc ctcggcgcgc ggcacgccct tgtcgatctc gaccagcgcg tactccacga
      841 gcaggatcga gttcttggtg acgatgccca tcagcatcag gatgccgatg atggcgggga
      901 gggagagcgc ctcgccgctc gcgaggagca ggccgagcgc gccgccgacc gagagcggca
      961 gggcggccat gatggtgagg ggctgggcga agtcgtggaa gaggagcacc agcacgcagt
     1021 acacgagcag cacgcccgcg acgatggcga tggcgaactc gccgaagagc tcgttcatgc
     1081 gctcggaatc gccgtagggc gtctgcttca cctcggcggg aaggttcttg atcgacggca
     1141 gcgcctggat ctgctcggtc accgtgccga gcggaacgcc cgcgatgtcg gccttgatgg
     1201 tgacgcttcg gcggcggtcg aagcgcgtaa tctgcgccgg gccgctgccc atctcgaccg
     1261 ccgcgacgtt gtcgagcggc acgctgccgc cgcccgcagt gggcacgcgc aggagcctga
     1321 gctcgtccag gtcaccgcgc gcttcgcgcg agatctgcgt gcggatcggg atactgcggt
     1381 cctcgagatt gaacttcgga agcagcgtcg aggtgtcgcc gatcgtgccg acgcgcgccg
     1441 tggtcgcgat gtcggccgaa gtgacgccga ggtcggcggc gcggctcgta tccgggcgga
     1501 tcgcaatctc gggccggcgc aggccggagg tcgccgccgg gttgccgacg ccggggatcg
     1561 agcgcatcgc gcgctccacc gcttctgcgg tgcggtccag caccgcgggg ctgtcgctca
     1621 cgagcacgag ctggaaggtc tcgccggctt cgccgccgcc gaagcgcacg cgcaggccgg
     1681 ggacggcggc gagctcgggc gcgatcagcc gctcgaactg gcgctgcgac agcttgcgct
     1741 ggttctcagg aagaagtttt accgtcaaag tgcctgcacg aacctcgccg gtgctcgagg
     1801 tgccgatggc gtcgagctgc gcgccggcgc cgatcacggc cagaacgccg gtcacttcct
     1861 tgtgctgcgc aatgatcctc cgcatggctt cggcagcggc caccgtctcc tcgagcggcg
     1921 tgccgggcgc gagctcgaag ttgatcgtgg tctgcccgcg gtcggaagcc gggatgaagg
     1981 ttttggcgag gaagggcccc attgccgccg acgcggcgag cagcacgccg ccggagagga
     2041 gcgcgagctt tcggtgcgcg aggccccatt ggacgaggcg cagataggcc tgcatggttt
     2101 tggaggtgtg atgttt
//
LOCUS       DASUNT010000657         5602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_522544, whole genome shotgun sequence.
ACCESSION   DASUNT010000657 DASUNT010000000
VERSION     DASUNT010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522544"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VFX94_13160"
     CDS             <1..869
                     /locus_tag="VFX94_13160"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX5769176.1"
                     /translation="LGVQIVLFFGFLFAFAVKVPMWPVHTWLPDAHTEAPTGGSVVLA
                     AILLKLGGYGFVRFTLPILPDAARELAWLMIALSLVAIAYIGLVTLVQTDMKRLIAYS
                     SVSHMGFVTLGFFIFNAYGVEGGLVQMISHGFVSAAMFLCVGVLYDRMHTRMIGDYGG
                     VVNTMPKFASFMMLFAMANAGLPATSGFVGEFMVIMGAMKASFWLAFVAATTLVLGAA
                     YTLWMYKRVIFGAVANSHVGALKDLNPREFMVLGALAAAVLAMGVYPRPFTEVMHASV
                     NELLRHVALTKL"
     gene            871..2376
                     /gene="nuoN"
                     /locus_tag="VFX94_13165"
     CDS             871..2376
                     /gene="nuoN"
                     /locus_tag="VFX94_13165"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEX5769177.1"
                     /translation="MNWTLLIIDHLPALPEGIVLAGACALMIFDLYVKDERRAATLWF
                     AQVILLLAFAATVFVLWGSVGKRLMIFNGLFVADAMGHLLKLVSYLAVSAALVYSRQY
                     LAERGLLRGEFVTLLLFSLLGMMVMISAASFLTLFLGLELMSLCLYALVALNRDSSLS
                     TEAAMKYFVLGALSSGLLLYGMSMIYGATGTLNIYEVAGHAARLAATPADRTFLVFGL
                     VFVVAGLAFKVGVVPFHMWIPDVYHGAPTAVTLIVGSAPKLAAFAMAVRLLVNGLIDL
                     AYDWQQMLVLLAVLSMALGNIAAIAQANLKRMLAYSTIAHMGFMLLGLLSGVVDGNRL
                     SAPDAYSSALFYVIVYVLMSLGAFGVLLYCSRAGFECENLDDVKGLNRTHPWFAFLML
                     LLMFSLAGIPPLAGFYAKLAVFSAAVNAGYIWLAVVAVIFSLIGAFYYLRVVKLMYFD
                     EPGDSRAGVAGMEGGSAGFRVALSVNGLALLALGIAPWPLMNACFEAIKAL"
     gene            2354..2908
                     /locus_tag="VFX94_13170"
     CDS             2354..2908
                     /locus_tag="VFX94_13170"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5769178.1"
                     /translation="MKPSKPSDLTERFVSGGQVYDGTLLKVYRDTVRLPDGTHGTREY
                     IRHPGAVAVVARFDDGAVLLERQYRYPGRREFIEIPAGKLEPGEPHLDTAKRELLEET
                     GYVAAEWTRLGVIHTAIGYADEAIEIFLARGLQYQAARLDAGEFLEVLRLPFGEAVAM
                     IRDGRISDSKTVAGLLWVDKWAAP"
     gene            complement(2905..3072)
                     /locus_tag="VFX94_13175"
     CDS             complement(2905..3072)
                     /locus_tag="VFX94_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769179.1"
                     /translation="MEAVLQPEVAIGNATQRFDEQGNLKDETSKKLIAQLVQNLVKKV
                     RLHRPEMKAAA"
     gene            3405..4097
                     /locus_tag="VFX94_13180"
     CDS             3405..4097
                     /locus_tag="VFX94_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX5769180.1"
                     /translation="MSEIRRSAERGHANHGWLDSFHSFSFADYYDPRHMGFGPLRVIN
                     EDRVQPGSGFGTHGHGDMEIISYVLQGALGHEDSMGNGSTIVPGDVQRMSAGTGVRHS
                     EYNYEKAGVTHFLQIWIEPDRTGIAPSYEQKHFTSEDKRGRLRLIASPDGREGSVTIH
                     QNAFVYAGLFHGPETASHSLALSRGYVHIARGKVKVNGHALSAGDALKTDGGSIEIQN
                     GENAEVLVFDLP"
     gene            4100..4378
                     /locus_tag="VFX94_13185"
     CDS             4100..4378
                     /locus_tag="VFX94_13185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5769181.1"
                     /translation="MDIGHEERGHRGAFVIERDGKRLAELTYTVAGSRVILDHTSVDD
                     ALRGTGAGKAMVQAAVEWARSGSRRLMPLCPFARSVFEKTPEFRDVLA"
     gene            complement(4375..>5602)
                     /locus_tag="VFX94_13190"
     CDS             complement(4375..>5602)
                     /locus_tag="VFX94_13190"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5769182.1"
                     /translation="LRRNLRLAFSLLLALVAGCAGAPSFPPVTAYEGARVIVGDGTVI
                     ENATLVVLGERIEQVGPAASVPVPAAATRVSLAGKTVMPMLIDTHVHLAAGREALIRD
                     LRRRAYYGVSAVLSLGLDGFDVLDLRRAPIAGAARFVSAGRGITMQEPGRTTVPYWVL
                     TEAEARAAVKELAAHNVDIVKIWVDTRDGQYRKLTPELYGAIIDEAHKRGLRVTAHIF
                     DLEDAKGLLRAGVDALAHGVRDRDIDEELVALFRERPQVVLTPNLPERGVKADLGWLK
                     QSLAPSQIEILERANTDRPIAQAFFGIQARNLARLNAAGVRITMGTDGNRPWGPHEEM
                     HDMVSAGMTPMEVIVAATRNSAEFLRLRDTGTLHPGKSADFIVMDANPLDDITNTRRI
                     SAVVLRGVPVDRSKPP"
BASE COUNT          807 a         1925 c         1852 g         1018 t
ORIGIN      
        1 cgctcggcgt gcagatcgtg cttttcttcg gcttcctctt cgccttcgcg gtcaaggtgc
       61 cgatgtggcc ggtgcatacc tggctgcccg acgcgcatac cgaggcgccg accggcggct
      121 cggtggtgct tgcggccatc ctgctgaagc tcgggggtta cggcttcgtt cgcttcacgc
      181 tgcccatcct gcccgacgcc gcgcgcgagc tcgcctggct gatgatcgcg ctgtcgctcg
      241 tcgcgatcgc ctacatcggc ctcgtaacgc tcgtgcagac cgacatgaag cgcctcatcg
      301 cctactcgtc ggtgtcgcac atgggattcg tgacgctcgg cttcttcatc ttcaacgcct
      361 acggagtcga aggcgggctc gtgcagatga tctcgcacgg cttcgtctcg gccgcgatgt
      421 tcctttgcgt cggcgtgctc tacgaccgca tgcacacgcg catgatcggc gactacggcg
      481 gggtggtgaa cacgatgccg aagttcgcct ccttcatgat gctcttcgcc atggcgaacg
      541 cggggctgcc ggccaccagc ggcttcgtcg gcgagttcat ggtcatcatg ggcgcgatga
      601 aggccagctt ctggcttgcc ttcgttgccg ccaccaccct cgtgctcggg gccgcctata
      661 ccctgtggat gtacaagcgc gtgatcttcg gggcggtggc gaactcgcac gtcggcgcgc
      721 tgaaggacct gaacccgcgc gagttcatgg tgctcggcgc gctcgccgcg gcggtgctcg
      781 ccatgggggt ctatccccgc ccgttcaccg aggtgatgca tgcctcggtg aacgagctgc
      841 tgcgccatgt cgcgctcacc aagctctagg atgaactgga ccctgctcat catcgaccac
      901 ctgccggcgc tgcccgaggg catcgtcctt gccggcgcgt gcgcgctcat gatcttcgac
      961 ctgtacgtca aggacgagcg ccgcgcggcc acgctctggt tcgcgcaggt gatcctgctc
     1021 ctcgcgttcg cggcgaccgt cttcgtgctg tgggggtcgg tcggcaagcg gctgatgatc
     1081 ttcaacggcc tcttcgtcgc cgacgcaatg gggcatctcc tcaagctggt ctcctacctc
     1141 gcagtgtcgg cggcgctcgt ctactcgcgc cagtacctcg ccgagcgcgg cctgctgcgc
     1201 ggggagttcg tgacgctgct cctcttctcg ctgctcggca tgatggtgat gatcagcgcg
     1261 gcgagcttcc tcacgctctt tctcggcctc gagctgatgt cgctgtgcct gtatgcgctc
     1321 gtggcgctga accgcgactc gtcgctctcc accgaggcgg cgatgaagta tttcgtgctg
     1381 ggcgcgcttt cctcggggct gctgctctat ggcatgtcga tgatctacgg ggcaacgggc
     1441 acgctcaaca tctacgaggt ggcggggcac gccgcccggc tcgccgcgac gccggcggac
     1501 cgcaccttcc tcgtgttcgg cctggtcttc gtggtcgccg ggctcgcgtt caaggtcggc
     1561 gtggtgccct ttcacatgtg gatccccgac gtttaccacg gcgcgcctac cgcggtaacg
     1621 ctcatcgtcg gctccgcgcc gaagctcgcc gcgttcgcca tggcggtccg gctcctggtg
     1681 aacgggctca tcgacctcgc gtacgactgg cagcagatgc tggtccttct cgcggtgctc
     1741 tccatggcgc tcggcaatat cgccgcgatc gcccaggcga acctcaagcg catgctcgcc
     1801 tactccacga tcgcgcacat gggcttcatg ctcctcggcc tgctttccgg cgtggtggac
     1861 ggcaaccggc tgagcgcccc cgacgcctac tcgagcgcgc tcttctacgt catcgtctac
     1921 gtgctcatgt cgctcggcgc gttcggcgtg ctgctctact gctcgcgcgc cggcttcgag
     1981 tgcgagaacc tggacgacgt gaaaggcctg aaccgcaccc acccctggtt cgccttcctc
     2041 atgctgctgc tcatgttctc gctcgccggc atcccgccgc tcgcgggctt ctacgccaag
     2101 ctcgcggtgt tctcggctgc ggtcaacgcg ggctacatct ggctcgcggt cgtggcggtg
     2161 atcttctcgc tcatcggcgc cttctactac ctgcgcgtgg tgaagctcat gtacttcgac
     2221 gagccgggcg actcgcgcgc cggcgtcgcc gggatggaag gcggcagcgc cggcttccgc
     2281 gtggcgctct ccgtcaacgg cctcgcgctc ctcgcgctcg gcatcgcgcc ctggccgctg
     2341 atgaacgcat gctttgaagc catcaaagcc ctctgacctg accgagcgct tcgtgagcgg
     2401 cggccaggtg tacgacggca cgctgctcaa ggtctaccgc gacacggtgc ggcttcccga
     2461 cggcacgcac gggacgcgcg aatacatccg ccaccccggc gcggtcgccg tcgtggcgcg
     2521 cttcgacgac ggcgcggtgc ttctcgagcg ccagtaccgc tatcccggcc gccgcgagtt
     2581 catcgagatc ccggccggca agctcgagcc cggcgagccg catctcgaca cggcgaagcg
     2641 cgagctgctc gaggaaacgg gttacgtcgc ggccgaatgg acacgcctcg gcgtgatcca
     2701 caccgcgatc ggatatgcgg acgaggcgat cgagattttc ctcgcgaggg ggctgcaata
     2761 ccaggcggct cggctggacg ccggggaatt tctggaagtg ctgaggcttc ctttcggcga
     2821 ggccgtggcg atgatccgcg acggccgcat cagcgactcc aagaccgtag cgggcctgct
     2881 ctgggtcgat aagtgggccg cgccttaggc ggcggctttc atctccgggc ggtgcaggcg
     2941 caccttcttc acgaggttct gcactagctg cgcgatcagc ttcttcgagg tctcgtcctt
     3001 caggtttccc tgctcgtcga agcgctgcgt cgcgttgccg atggccacct cgggctgcag
     3061 caccgcttcc atgttgaggg cgtggcgagg aggattgcgt cggcgccgcg tatgcgctgc
     3121 ttgagctccg ccacctggcg cggcgggctg cgctcctcgt cctggttgaa gaccggaatg
     3181 ccgtcgaggt cgaacacctc gatcgtcgcg tagttcgggc aaagctcgcg cgcggcgcgc
     3241 agcgcgctgc ggttgtagga acctttgcga aggcttcccg cgatgccgag aattttcagt
     3301 gggtccgcca tcatcgtcct ccggggggta aaccccaccg gatgccagaa acatgcccgc
     3361 gggcgggctt gcaaaaacgc tcggccgccg caaacttgca tgccatgagc gaaatccgcc
     3421 gcagcgccga gcgcggtcac gccaaccacg gctggctcga ctccttccac agcttttcct
     3481 tcgcggacta ctacgacccg cgccacatgg gcttcggccc gctgcgggtg atcaacgagg
     3541 accgggtgca acccggctcg gggttcggca cccatggcca cggcgacatg gagatcatca
     3601 gctacgtcct ccagggcgcg ctcggccacg aggacagcat ggggaacggc tccaccatcg
     3661 tgccgggcga tgtgcagcgc atgagcgcgg gcaccggcgt gcgccacagc gagtacaact
     3721 acgagaaggc cggcgtcacg cacttcctcc agatctggat cgagccggac aggaccggca
     3781 tcgcgccctc ctacgagcag aagcacttca cctccgagga caagcgcggg cgcctgcggc
     3841 tcatcgcttc gcccgacggc cgcgaaggct cggtgaccat ccaccagaac gccttcgtgt
     3901 atgccggact cttccacggt cccgagaccg cgagccacag cctcgccctg agtagaggct
     3961 acgtgcacat agcgcgcggg aaggtgaagg tgaacggcca tgcgctctcg gcgggcgatg
     4021 cgctgaagac cgacggcggt tcgatcgaga tccagaacgg cgagaacgcc gaagtgctgg
     4081 tcttcgacct gccgtagcca tggacatcgg acacgaagag cgagggcacc gcggcgcctt
     4141 cgtcatcgag cgcgacggca agcgattggc ggagctgact tacaccgtag cgggcagccg
     4201 ggtcatcctc gaccacacga gcgtggacga cgcgctgcgc ggcactgggg ccggcaaggc
     4261 gatggtccag gcggcggtcg aatgggcgcg ctcgggcagc cggcgcctca tgccgctttg
     4321 ccccttcgcg cgctcggtct tcgagaagac cccggaattc cgcgacgtgc tcgcttaggg
     4381 cggtttcgag cgatcaacgg gcacgccgcg cagcacgacc gcggaaatgc gccgggtgtt
     4441 cgtgatgtcg tcgagcgggt tcgcgtccat cacgatgaag tccgcgctct tgccggggtg
     4501 cagcgtgccg gtgtcccgca gccgcaggaa ctcggccgag ttgcgcgtcg cggcgacgat
     4561 gacctccatc ggcgtcatgc cggccgacac catgtcgtgc atttcctcgt gcgggcccca
     4621 cgggcggttg ccgtcggtac ccatggtgat gcgcacgcca gcggcgttca gccgcgccag
     4681 gttgcgcgcc tggataccga agaacgcctg cgcgatcggc cggtcggtgt tcgcgcgctc
     4741 gaggatttcg atctggctcg gcgcgaggct ctgcttcagc cagccgaggt cggccttcac
     4801 gccgcgctcc ggcaggttgg gtgtgagcac gacctgcggc cgctcgcgga acagcgcgac
     4861 gagctcctcg tcgatgtcgc ggtcgcgcac gccatgcgcc agtgcgtcga ccccggcgcg
     4921 gaggagcccc ttggcgtcct caaggtcgaa gatgtgcgcg gtgacgcgca gcccgcgctt
     4981 gtgcgcctcg tcgatgatcg cgccatagag ctcgggcgtg agcttgcgat actggccgtc
     5041 gcgcgtatcg acccagatct tgacgatgtc gacattgtgc gcggcgagct ccttcaccgc
     5101 ggcgcgcgcc tcggcttcgg tgagcaccca gtagggcacg gtggtgcgtc cgggctcctg
     5161 catcgtgatc ccccggccgg cgctcacgaa gcgcgcggcg ccggcaatcg gcgcgcggcg
     5221 caggtcgagc acgtcgaagc cgtcgagccc gaggctgagc accgcgctta cgccgtagta
     5281 cgcgcgccgc ctgaggtcgc ggatcagcgc ctcgcgtccc gccgcgagat gcacatgtgt
     5341 atcgatgagc atcggcatca ccgtcttgcc cgcgaggctg acccgcgtcg cggctgcggg
     5401 aaccggcacg ctcgccgccg gcccgacctg ctcgatcctc tcgcccagca cgacgagcgt
     5461 ggcgttttcg atgacggttc cgtcgccgac gatcacgcgg gcgccttcgt aggccgtgac
     5521 cggcgggaag gagggagccc ccgcgcatcc cgcgacgagg gcaagaagca gtgaaaacgc
     5581 tagccgcaga ttcctacgta gc
//
LOCUS       DASUNT010000658         3095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_784345, whole genome shotgun sequence.
ACCESSION   DASUNT010000658 DASUNT010000000
VERSION     DASUNT010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784345"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1689)
                     /locus_tag="VFX94_13195"
     CDS             complement(40..1689)
                     /locus_tag="VFX94_13195"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5769183.1"
                     /translation="MHRLLVAMALGCAGIGACAQPAEDLSEPTLYEFLLGEIAVQRGD
                     LPLAAKTYLDLARRTRDPRVARRAVEVANQAKEPQLALEAARLWNELEPSAHSLQILA
                     ATLVATKRVDEAGPVIEKLLSTEGVNLENAFAQLNRLLAGNPDKAANQRVIKGIAAKY
                     PQLPQAHLAVAQAAAIAGDNDAAIASAREAQKLRPDWELAVLLEAQVLQQRSPAAAAK
                     RLGEFVEAYPGSREARLAYARLLVMEKRLPEARKQFEAVASASPKNPDVLYAVGLLAF
                     QSKDYAAAEDYMKRVLGLGYRDPEGVRYLLGQMAEEQKMWPRAIQWYETIETGDHALP
                     ARMRTANAMAKQGQLDEARAFLKRVANDNPEDAVQLTVAEAQLLRDAQRHAEAFSLLS
                     EALGKQPEQPDLLYDIALTAEKLERYDLLETHLRKLIEVKPDHAHAYNALGYSFAERN
                     TRLPEARKLIEKALELAPEDYFIIDSLGWVLYREGDLKGAARELRRAYGGRPDAEIGA
                     HLGEVLWMLGERDEARRIWDESLKTAPDNETLQKTIKRLRK"
     gene            1756..2565
                     /gene="mutM"
                     /locus_tag="VFX94_13200"
     CDS             1756..2565
                     /gene="mutM"
                     /locus_tag="VFX94_13200"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX5769184.1"
                     /translation="MPELPEVEVTRRGLAPQLTGRTISAVAVREPRLRWPVPRDVLCL
                     AGRTVRTIRRRGKYLLIDCGDGHLIMHLGMSGSLRVLPPGTPAQKHDHFDLVLGDRVL
                     RLRDPRRFGAVLWAAGDADAHPLLAHLGIEPLSRALDARRLYALTRAHRTAIKLFLMD
                     GRRIVGVGNIYANESLFRAGIDPRKPARRLSAGQCEKLAKAIKDTLRAAIRSGGSTLR
                     DFVGADGAAGYFQSRYWVYDRAGKECRRCGKLIRKRQQGQRSTYYCPLCQK"
     gene            complement(2576..3052)
                     /gene="coaD"
                     /locus_tag="VFX94_13205"
     CDS             complement(2576..3052)
                     /gene="coaD"
                     /locus_tag="VFX94_13205"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX5769185.1"
                     /translation="MIKAVYPGTFDPLTRGHEDLVRRAATLFDTIVLAVAEAKAKRPF
                     FTLEERISMAREVLADLANVEVVGFTGLLTDFVRKQGSRVVLRGLRAVSDFEYEFQLA
                     GMNRHLYRGMETVFLTPSEQHMFISATLVREIAMLGGDVSEFVHPTVAARLRQKRG"
BASE COUNT          468 a         1069 c         1036 g          522 t
ORIGIN      
        1 cgcacgcagc cagaaacgct gcgagcaata caaccgctct cacttgcgca gtcgcttgat
       61 ggtcttctgc agggtttcgt tgtcgggcgc ggtcttgagc gactcgtccc agatgcgccg
      121 tgcttcgtcg cgctcgccca gcatccagag cacttcgccg aggtgcgcgc cgatctcggc
      181 gtcgggccgg ccgccgtagg cgcggcgcag ctcgcgcgcc gcgcccttga gatcgccttc
      241 gcgatacagg acccagccca ggctgtcgat gatgaagtag tcctcgggtg cgagctcgag
      301 cgccttctcg atcagcttcc tcgcctcggg cagccgggtg ttgcgctcgg caaacgaata
      361 gccgagcgcg ttgtaggcgt gcgcgtggtc gggcttcacc tcgatcagct tgcgcaggtg
      421 ggtctcgaga aggtcgtaac gctcgagctt ctcggcggtg agcgcgatgt cgtacaggag
      481 atcgggctgc tccggctgct tgccgagcgc ctcggaaagg agcgaaaaag cctctgcatg
      541 ccgctgcgcg tcgcgcagga gctgcgcctc ggcgacggtg agctggaccg catcctcggg
      601 gttgtcattt gcaacgcgct tcagaaacgc gcgcgcctcg tcgagctgtc cctgcttcgc
      661 catcgcgttc gcggtgcgca tgcgcgcggg cagggcatgg tcgccggtct cgatggtctc
      721 gtaccactgg atcgcgcgcg gccacatctt ctgctcttcg gccatctggc cgagcaggta
      781 gcgtacgccc tcggggtcgc gatagcccag accgaggacg cgcttcatgt aatcctcggc
      841 cgcggcatag tccttcgact ggaacgcgag cagccctacg gcgtagagca cgtccggatt
      901 cttcggcgaa gcacttgcca cggcttcgaa ctgcttgcgc gcctcgggca gacgcttctc
      961 catcaccagc aggcgcgcat aggcgagccg cgcctcgcgc gagccgggat aggcctcgac
     1021 gaactcgccg agccgcttcg ccgccgcagc gggcgagcgc tgctgcagca cctgagcctc
     1081 gagcagcacc gcgagctccc agtcgggccg cagcttctgc gcctcgcggg cagaggcgat
     1141 cgcggcgtcg ttgtcgccgg cgatggccgc ggcctgcgcg acggcaagat gcgcctgcgg
     1201 cagctgcggg tatttcgccg cgataccctt gatcacgcgc tggttggctg ccttgtcggg
     1261 attgccggcg agcaggcggt tgagctgcgc gaacgcgttc tccaggttga cgccttcggt
     1321 cgaaagcagc ttctcgatca ccggcccggc ttcgtccact cgcttggtcg caaccagcgt
     1381 cgcggcgaga atctgcagcg agtgcgccga gggctcgagc tcgttccaca gccgcgcggc
     1441 ctccagcgca agctgaggct ccttcgcctg gttggcgacc tccaccgcgc ggcgcgccac
     1501 gcgcggatcg cgcgtgcggc gcgcgagatc gaggtaggtc ttggcggcaa gagggaggtc
     1561 gccgcgctgc acggcgatct cgccgagcag aaactcgtac agcgtgggct cgctcagatc
     1621 ctcggccggc tgggcgcacg ccccgatgcc agcgcagcca agcgccatcg ccacaagaag
     1681 tctgtgcatt gctttggtca tgctgggtgc gggccatctt aggtatattt ggtgccccgc
     1741 acaagcgcac ttgtcatgcc ggagctccca gaagtcgaag tgacgcggcg cggccttgcg
     1801 ccgcagctca ccgggcgcac gatcagcgcc gtagcagtgc gtgagccgcg cctgcgctgg
     1861 ccggtgccgc gcgacgtgct gtgcctcgcc gggcgcacgg tgcgcacgat ccgccgccgc
     1921 ggcaagtacc tgctcattga ttgcggcgac ggccatctca tcatgcacct cggcatgtcg
     1981 ggcagcctgc gtgtgctgcc tcccggcacg ccggcgcaga agcacgacca tttcgatctc
     2041 gtgctcggcg atcgcgtgct gcggctgcgc gacccgcggc gcttcggcgc ggtgctgtgg
     2101 gctgccggcg acgcagacgc gcacccgctt ctcgcgcatc tcggcatcga gccgctctca
     2161 cgcgcgctgg acgccaggcg cctgtacgcc ctcactcgcg cgcaccgcac ggcgatcaag
     2221 ctcttcctca tggacggccg gcgcattgtc ggcgtcggca acatctacgc gaacgagagc
     2281 ctctttcgcg ccggcatcga cccgcgcaag cccgcgcgcc ggctgagcgc cgggcaatgc
     2341 gaaaagctcg cgaaggccat caaggacacg ctgcgcgcgg cgatccgctc gggcggctcg
     2401 acgctgcgcg atttcgtggg cgccgacggc gccgcgggat actttcagag ccgctactgg
     2461 gtgtacgacc gcgcgggaaa ggaatgccgc cgctgcggca agctcattcg caagcgccag
     2521 cagggacaac gctcgaccta ctactgcccg ctgtgccaga agtagatagg ccggttcagc
     2581 cgcgtttctg cctcagcctc gcggcgaccg tcgggtgcac gaactcactc acgtcgccgc
     2641 ccagcatggc gatctcgcgc accagcgtgg cggagatgaa catatgctgc tcggagggcg
     2701 tgaggaacac cgtctccatc ccgcggtaga gatggcggtt cattccggcg agctggaact
     2761 cgtactcgaa gtcggacacg gcgcgcaacc cgcgcagcac gacgcgcgag ccttgcttgc
     2821 gcacgaagtc ggtcagcagg ccggtgaagc cgacgacctc tacgttcgcc aggtcggcaa
     2881 gtacctcgcg cgccatcgat atgcgctcct ccagagtgaa gaacgggcgc ttggcctttg
     2941 cttccgctac ggcgagcacg atcgtgtcga agagcgtggc cgcgcggcgc accaggtcct
     3001 cgtggccgcg cgtgagcgga tcaaacgttc ccgggtagac cgccttgatc atcgccgcct
     3061 ctccattcca agagctggta gctcacctgc ccggc
//
LOCUS       DASUNT010000659         2845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1223187, whole genome shotgun sequence.
ACCESSION   DASUNT010000659 DASUNT010000000
VERSION     DASUNT010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223187"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="VFX94_13210"
     CDS             complement(<1..1444)
                     /locus_tag="VFX94_13210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5769186.1"
                     /translation="MKEGPDPFFEEKCSILVVDDLEEKLLVFRTILEELGQNLVTVRS
                     GSEALREVLAREFAVILLDVNMPDIDGLETARLIRQYKKSAHTPIIFITAYADEMQTA
                     QGYSLGAVDYILSPVVPEVLRSKVRVFVDLYQMQQRTRAMAGEQVARATAEAAKLAAE
                     ETTRRSNFLARASHELGASLDLEQSMRRLLDLVVPQVAQIAALVVDADTEKPLIFLKT
                     EKNTQAVPGYDALPQDLREALQRVVREGLPLEGDTVCYPLLAGERTLGALALASPRGL
                     EPRDLVTLQELASRAAIALENARLYSSLQRESVRSRQAEERLQDANRRKDEFLAMLSH
                     ELRNPLAPIRNAVEVIRRLAPPDPKLTMARDVVDRQVSLLARLVDELLDVSRISQGKI
                     SLRREPVDLARVVAHSVETARPLIDARAQTLAVSVPAAPVWLSADFARLSQVVSNLLN
                     NASKYTPEGGRIELSAAAAEGEASITVRDNG"
     gene            complement(1441..>2845)
                     /locus_tag="VFX94_13215"
     CDS             complement(1441..>2845)
                     /locus_tag="VFX94_13215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5769187.1"
                     /translation="SRELSKLLGGEIKLASAPGAGSVFTLYLPQTYLPSRQARPRLIT
                     EKIPAPAPVESVSPDEPAELQLVNEAGDDRDEVQPGDQVILIVENDLGFAKILLEAAR
                     EKRFKGVVASSGAAALAMVREYHPAVMTLDIFLPDMQGWRVLERLKADLATRHIPVCV
                     VSTDEARDRALDSGAIGFISKPLVSRDLVDRAIERLKAFVSRPAKQVLLLMPDGAARS
                     EIVERLGAADVPMLVAWSGDEALRLLRTSQVDCLVVGENAADLEPQDVLDVLAEQPVT
                     QILPVVFHGQNPLAARWKNSEGAFALREAHTRERLLDYIYFFLHRSAASMPAAEREFL
                     DNLHSTHRILEGKRALIVDDDMRNIFALATVLDEQGMEIVSANNGRDAIRKVEADADI
                     DVVLMDIMMPEMDGITTIKEIRKLPKGADLPIIAVTAKAMKGDREKCIEAGAWDYLSK
                     PVDRLHLLAVLRGWLHR"
BASE COUNT          407 a          972 c          989 g          477 t
ORIGIN      
        1 cgccgttgtc gcgcaccgtg atgctcgctt caccctcggc ggcggccgcc gagagctcga
       61 tgcgcccgcc ctcgggtgtg tacttggacg cgttgttgag caggttcgac accacctgcg
      121 agaggcgcgc gaagtcggcc gacagccaga ccggcgccgc cggcaccgac accgcaagcg
      181 tctgggcgcg cgcgtcgatc agcgggcgcg cggtttccac gctgtgcgcc acgacgcgcg
      241 cgagatcgac cggctccctc cggagcgaga tctttccctg gctgatgcgc gacacgtcga
      301 gcagctcgtc gaccagccgc gcgagcagcg acacctggcg gtcgaccacg tcgcgcgcca
      361 tggtgagctt cggatcgggc ggggcgaggc gccggatcac ctcgaccgcg ttgcggatcg
      421 gggcgagcgg gttgcgcagc tcgtgcgaca gcatggcaag gaactcgtcc ttgcgccggt
      481 tggcgtcctg cagcctctcc tccgcctggc gcgagcggac gctctcgcgc tgcaggctgg
      541 agtacagccg cgcgttctcg agcgcgatcg cggcccggct cgccagctcc tgcagcgtga
      601 cgaggtcgcg cggctcgagc ccgcggggcg atgcgagcgc gagcgcgccg agcgtgcgct
      661 cgccggcgag cagcggatag cacacggtgt cgccttccag gggcaggccc tcgcgcacga
      721 cgcgctgcag cgcttcgcgc aggtcctgcg gcagcgcgtc gtaaccggga acggcctggg
      781 tgttcttttc ggttttcagg aatatcagcg gtttttccgt gtctgcgtcg acgaccagcg
      841 cggcgatctg cgccacttgc ggcaccacca ggtcgagcag ccgccgcatg ctctgctcca
      901 ggtcgagcga ggcgccaagc tcgtggctcg cgcgcgcgag gaaattcgag cgccgcgtgg
      961 tttcttcggc ggcgagcttc gccgcctcgg cggtcgcgcg cgccacctgt tcgcctgcca
     1021 tggcgcgcgt gcgctgctgc atctggtaga gatcgacgaa cacgcgaacc ttgctgcgca
     1081 gcacctcggg gacgaccggc gagaggatgt aatccaccgc gccgagcgag tagccttgcg
     1141 ccgtctgcat ctcgtcggcg taggcggtga tgaagatgat cggcgtatgc gccgacttct
     1201 tgtactggcg gatgaggcgc gcggtctcca gcccgtcgat gtcgggcatg ttgacgtcga
     1261 gcaggatgac ggcgaattcg cgcgcgagta cctcgcgcag cgcttccgac cccgagcgca
     1321 ccgtcaccag gttctgtccg agctcctcca ggatcgtccg gaagacgagc agcttttcct
     1381 cgaggtcgtc gaccaccagg atgctgcatt tctcctcgaa aaaggggtct ggaccctctt
     1441 tcaccggtgg agccagccgc gcaggaccgc gagcaaatgc agccggtcga ccggcttgga
     1501 gaggtaatcc cacgcgcccg cttcgatgca tttctcgcgg tcgcccttca ttgccttggc
     1561 ggtcaccgcg atgatcggca ggtcggcgcc tttcggcagc ttgcggatct ccttgatcgt
     1621 ggtgatgccg tccatctccg gcatcatgat gtccatcagg acgacgtcga tgtcggcgtc
     1681 ggcctcgacc ttcctgatcg cgtcgcggcc gttgttcgcc gagacgattt ccatgccctg
     1741 ctcgtcgagc accgtggcga gcgcgaagat gttgcgcatg tcgtcgtcga cgatgagcgc
     1801 gcgcttgccc tccagtatcc ggtgcgtcga gtgcaggttg tcgaggaact cgcgctcggc
     1861 cgccggcatg ctggcggcgc tgcggtgcag gaagaaatag atgtagtcga gcagccgctc
     1921 gcgggtgtgc gcctcgcgca gcgcgaacgc gccctcgctg ttcttccagc gcgcggcgag
     1981 cgggttctgg ccgtggaata ccaccggcag tatctgcgtc accggctgct cggcgaggac
     2041 atcgagcacg tcctgcggct cgaggtcggc cgcgttctcg cccaccacca ggcagtcgac
     2101 ctggctcgtg cgcagcaggc gcagcgcttc gtctcctgac caggcgacga gcatcggcac
     2161 gtcggcggcg ccgagccgct cgacgatctc gctgcgcgcc gcgccatcgg gcatgaggag
     2221 cagcacctgc ttcgccgggc gcgagacgaa ggccttcagg cgctcgatcg cccggtcgac
     2281 gagatcgcgc gatacgagcg gcttggagat gaagccgatc gcccccgagt cgagcgcgcg
     2341 gtcgcgcgcc tcgtcggtcg ataccacgca caccgggatg tgccgcgtcg cgagatccgc
     2401 cttcaggcgc tcgagcaccc gccagccctg catgtcgggc aggaagatgt cgagcgtcat
     2461 caccgccggg tggtactcgc gcaccatggc gagcgccgcc gcgccgctgg aggcgaccac
     2521 gcccttgaag cgcttctcgc gcgccgcttc gagcaggatc ttggcgaagc cgagatcgtt
     2581 ctcgacgatg aggatcacct ggtcgccggg ctggacctca tcgcggtcgt cgcccgcctc
     2641 gttgacgagc tgcagctccg ccggctcatc gggcgaaacc gactcgaccg gcgcgggagc
     2701 ggggatcttc tcggtgatga gccgcggcct cgcctggcgc gagggcaggt acgtctgcgg
     2761 caggtagagc gtgaacacgc tgcccgcgcc cggcgcgctc gcgagcttga tctcgccgcc
     2821 gaggagcttg gagagctcgc gcgag
//
LOCUS       DASUNT010000660         4975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_259284, whole genome shotgun sequence.
ACCESSION   DASUNT010000660 DASUNT010000000
VERSION     DASUNT010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259284"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFX94_13220"
     CDS             complement(<1..221)
                     /locus_tag="VFX94_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5769188.1"
                     /translation="MNFLRFEHGETIDLLRESVREFTAREIAPRAAHIDQTNEFPSEL
                     WRKLGALGVLGITVEEEYGGTAMGYLAHI"
     gene            complement(299..682)
                     /locus_tag="VFX94_13225"
     CDS             complement(299..682)
                     /locus_tag="VFX94_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5769189.1"
                     /translation="METERAEFSISELAREFDVTPRAIRFYEDQGLLAPRREGQRRVY
                     SPRDRTRLKLTLRGKRLGLTLSEIRDLIDMYEPGRDERPQLERFLAVLESHKSSLLQQ
                     RDDIEAQLSEIQAFEKKVRKQLKRG"
     gene            709..1737
                     /locus_tag="VFX94_13230"
     CDS             709..1737
                     /locus_tag="VFX94_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5769190.1"
                     /translation="MSAQAISSLTYPFPEVPAPGTTIEVAPGIRWLSMPLPFKLDHIN
                     LWLVEEEGGWTVVDTGIGNPETRALWEQILGQGHVKRVILTHYHPDHAGNAAWLCERY
                     GAELWTTQGEYLTAHAVRTAGAGYTPDAVLAVFRKNGLDDERAARMRSSRNRYAELVP
                     GFPHSYRRIIEGDEVPMGGRRWRAIVGHGHAPEHLSLFSKELNTVIAGDMLLSTISTN
                     VSVWSIDPEGDPLRLFLDSIARYRGLPADVLVLPSHGKPFRGAHRRIGELEQHHRERL
                     EELSQCLHSPKSAAELLGVLFRRPLDAHQTFFAMGEAIAHLHYLYYAGRAERAMGHDG
                     IMRYAIRP"
     gene            1747..3549
                     /gene="phaC"
                     /locus_tag="VFX94_13235"
     CDS             1747..3549
                     /gene="phaC"
                     /locus_tag="VFX94_13235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEX5769191.1"
                     /translation="MKAPENLENQELAKVCHDVAERSSRILGDFVQKQAETVSSAVRD
                     EMGIAKAFMDLYARMATDPGAIAALSMNLWLDHFRLWQSSWIKLFGMQAPPVAEPDKG
                     DSRFKDEDWSKNFLFDLIKQSYLISARHIREAVAKVDGLPAESEKKVAFFTRQYVDAL
                     APSNFLLTNPQVLRETVSSGGQNLVRGLKNLLADLEKGGGQLRISMTDESAFQLGKNV
                     ATTPGKVVYQTDLMQLIQYAPLTEQVYRRPLVIIPPWINKYYILDLRATNSFIRWAVQ
                     QGHTVFVVSWVNPDARLGQKGFEDYMLEGSLAALDAVQRGTGATEVNFIGYCLGGTLL
                     GATLAYLAEKKQHSVASATFFVSLLDFSQPGELGVFIDEEQVANLERKMNERGYLEGS
                     EMAGTFNLLRANDLVWSFVVNNYLLGKDPFPFDLLYWNADSTRMPARMHSFYLRNMYI
                     KNLMGVRGGIELAGVPIDLSRVTLPSYFISTVEDHIAPWKTTYKGAKYLRGLVRFVLG
                     GSGHIAGIVNPPAAKKYHYWTNEAMPDTPEEWFKGATQHPGSWWEDWQRWIEPHGGGK
                     APARTPGEGALRAIEDAPGSYAMTRIAQKTRAKR"
     gene            complement(3620..4372)
                     /locus_tag="VFX94_13240"
     CDS             complement(3620..4372)
                     /locus_tag="VFX94_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5769192.1"
                     /translation="MRVIVTGASSGIGEALARRYAREGVVLGLISRRRVDPALPGEVV
                     SYALDVNNERALTTAAQDFIQRYGPPDIVIANAGIASGTDGAEIGDVAKLRKIVETNV
                     VGLAATLAAFAPAMRSAGRGTLVGIASIAGFRGLAGSGAYCASKSAAITWMESLRAEL
                     YGSGVSVVCICPGYIDTPMTRINRFRMPFLLSADEAARRIQRAIAAKRRLAIIPWQMA
                     LLSLLLRMMPGWLFDRLASRAPRKPRTSRFEL"
     gene            complement(4391..>4975)
                     /locus_tag="VFX94_13245"
     CDS             complement(4391..>4975)
                     /locus_tag="VFX94_13245"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEX5769193.1"
                     /translation="ALVATLSLAPLAALGQRSPDYYELNPPQPVETTDKIEVLEFFWY
                     GCIHCYNLEPKLESWLKTLPKDVEFRRVPAVFNDRWAHDATIYYAFEALGLVDKLHRP
                     LFDAIHRDRLRTDDSKQLSAWLEKHGVDPKKFETTAKSFGVQSKTKRAIRLTTDYKID
                     GTPAMAVQGRYTVPSSEAMLAVVNQLVTQVRKQK"
BASE COUNT          881 a         1619 c         1612 g          863 t
ORIGIN      
        1 atgatgtgcg cgaggtagcc catcgcagtg ccgccgtatt cctcctcgac cgtgatgccg
       61 agaacgccga gcgcgccgag cttgcgccag agctcggaag gaaactcgtt cgtctggtcg
      121 atgtgcgcgg cgcgcggggc gatctcccgc gcggtgaact cgcgcaccga ctcgcgcagc
      181 aggtcgatgg tctcgccatg ctcgaatctg agaaagttca tgaccgctcg cttcgactcc
      241 ggtctgtcgg gttcatgagg ctgggccggg aggagcctac ttaacgttta cgttaacgtc
      301 aacctcgctt cagctgcttg cggaccttct tctcgaacgc ctgaatctcg gaaagctgcg
      361 cctcgatgtc gtcgcgctgc tgcagcaggc tcgatttgtg cgactcgagc acggcgagaa
      421 agcgctcgag ctgcggccgc tcgtcgcgcc cgggctcgta catgtcgatg aggtcgcgga
      481 tctcggagag cgtgagtccc aggcgcttgc cgcgcagcgt gagcttgagc cgggtgcggt
      541 cgcgcggcga gtagacgcgg cgctggccct cgcgccgcgg cgcgagcagg ccctggtcct
      601 cgtagaagcg gatggcgcgc ggggtgacat cgaactcgcg cgcgagctcg ctgatcgaga
      661 attcggcgcg ttccgtttcc atggtaactt cgattttgat tctagcggat gtcagctcag
      721 gcgatctctt cgctcactta tccgttcccc gaagtgcccg cgcccggcac gacgatcgaa
      781 gtcgcgcccg ggatccggtg gctttcgatg ccgctgccgt tcaagctcga ccacatcaac
      841 ctctggctgg tcgaggaaga gggcggctgg acggtggtcg acaccgggat cggcaacccc
      901 gagacccgcg ctctctggga gcagatcctc gggcaaggac acgtgaagcg cgtcatcctc
      961 acgcattacc accccgacca cgccggcaat gcggcatggc tttgcgagcg ctacggcgcc
     1021 gagctgtgga caacgcaggg ggagtacctg accgcgcacg ccgtgcgcac cgcgggcgcc
     1081 ggctacacgc ccgacgcggt gctcgccgtg ttccgaaaaa acggcctcga cgacgagcgt
     1141 gccgcgcgca tgcgcagctc gcgcaaccgc tacgcggagc tcgtgcccgg ctttccgcac
     1201 tcgtaccgcc gcatcattga aggcgacgag gtgccgatgg gcgggcgtcg ctggcgcgcc
     1261 atcgtcggcc acggtcacgc gccggagcac ttgtccctgt tctccaaaga gctgaacacc
     1321 gtgatcgccg gagacatgct gctctccacc atctccacca acgtgagcgt ctggtcgatc
     1381 gaccccgaag gcgatccgct gcgcctgttt ctcgactcca tcgcgcgcta ccgcgggctg
     1441 cccgcggacg tgctcgttct gccttcgcac ggcaagcctt tccgcggcgc gcatcggcgc
     1501 atcggcgagc tcgagcagca ccaccgtgag cggctcgagg agttgagcca gtgcctgcac
     1561 agcccgaaaa gcgccgccga gctgctcggc gtgctgttcc gcaggccgct cgacgcgcat
     1621 caaacatttt ttgcaatggg cgaggcgata gcccatctgc actacctgta ctatgcgggc
     1681 cgtgccgagc gcgccatggg ccatgatggc atcatgcgct atgcgatccg cccatagcag
     1741 aaggcgatga aagcgcccga aaatctcgaa aaccaggagc tcgccaaggt gtgccacgac
     1801 gtcgcggaac gctcctcgcg catcctgggc gacttcgtgc agaagcaggc cgagacggtg
     1861 tcctccgcgg tgcgcgacga gatggggatc gccaaggcgt tcatggacct ctacgcgcgc
     1921 atggcgaccg acccgggcgc aatcgccgcg ctgtcgatga atctgtggct cgaccacttt
     1981 cgcctgtggc agtcgagctg gatcaagctc ttcggcatgc aagcccctcc ggtcgccgag
     2041 cccgacaagg gcgattcgcg cttcaaggac gaggactggt cgaagaactt tctcttcgac
     2101 ctcatcaagc agtcctatct catcagcgca cgccatatcc gcgaggccgt ggcgaaggtg
     2161 gacggcctgc cggccgaatc ggagaaaaag gtcgcatttt ttacccgtca gtacgtcgac
     2221 gcgctcgcgc cctccaactt tctgctgacc aatccgcagg tgctgcgcga gacggtgtcg
     2281 agcggaggcc agaacctggt gcgcgggctg aagaaccttc tcgccgatct cgaaaaaggt
     2341 ggcggtcagc tgcgcatctc gatgaccgac gagagcgcct tccagctcgg caagaacgtc
     2401 gccaccacgc ccggaaaggt cgtctaccag accgacctca tgcagctcat ccagtacgcc
     2461 ccgctcaccg agcaggtgta ccggcgcccc ctcgtcatca tcccgccctg gatcaacaag
     2521 tactacatcc tcgacttgcg ggctacgaat tccttcatcc gctgggcggt gcagcagggg
     2581 cacacggtgt tcgtcgtgtc ctgggtgaac ccggacgcgc gcctcgggca gaaaggcttc
     2641 gaggactaca tgctcgaagg ctcgcttgcc gcgctcgacg cggtgcagag gggaaccgga
     2701 gcgacggaag tcaacttcat cggctactgc ctcggcggca cgttgctcgg cgccacgctc
     2761 gcctacctcg ccgagaagaa gcagcacagc gtggcgagcg ccacgttttt cgtcagcctg
     2821 ctcgatttct cgcagccggg cgagctcggc gtcttcatcg atgaagagca ggtggcgaac
     2881 ctcgagcgca agatgaacga gcgcggctat ctcgaagggt cggagatggc cgggaccttc
     2941 aacctgctgc gcgcgaacga cctcgtgtgg tcgttcgtgg tgaacaacta cctgctcggc
     3001 aaggatccct tcccgttcga cctgctgtac tggaacgccg actccacgcg catgccggcg
     3061 cgcatgcaca gcttttacct gcgcaacatg tacatcaaga accttatggg cgtccgcggc
     3121 gggatcgagc tcgccggcgt gccgatcgat ctttccaggg tgacgctgcc ttcgtacttc
     3181 atctcgaccg tcgaagacca catcgctccg tggaagacga cctacaaggg agcgaaatac
     3241 ctgcgcgggc ttgtgcgctt tgtgctcgga ggctccggcc acatcgcggg catcgtgaac
     3301 ccgcccgcgg cgaagaagta ccactactgg accaacgaag ccatgcccga cacgccagag
     3361 gagtggttca agggcgccac acagcaccca gggtcgtggt gggaagactg gcaacgctgg
     3421 atcgagccgc atggcggcgg aaaagccccg gcgcgaaccc cgggcgaggg cgcgctgcgc
     3481 gcgatcgaag atgccccggg cagctacgcg atgacgcgca tagcgcagaa aacgagggcg
     3541 aagcgctaac ggaattcggg gtcagggtcg caacttgttt tcgcgtacgc aagaacaagt
     3601 tgcgaccctg accccgaact cagagctcaa accgggacgt tctcggcttg cgcggggcgc
     3661 gcgaagcaag ccggtcgaaa agccagcccg gcatcattcg cagcaacagc gatagcagtg
     3721 ccatctgcca cggaatgatc gcgagccggc gcttcgccgc aatggcgcgc tggatgcgcc
     3781 gtgccgcctc gtcggccgaa agcaggaacg gcatgcggaa gcggttgatg cgggtcatgg
     3841 gcgtgtcgat gtagcccggg cagatgcaca ctacggacac cccgctgccg taaagctcgg
     3901 cgcgcaggct ttccatccag gtgatcgccg ccgatttcga agcgcagtaa gcgccgctgc
     3961 ccgcgagacc ccggaaaccg gcgatgctcg cgattccgac gagcgtgccg cggccggcgc
     4021 tgcgcatggc gggcgcgaag gcggcgaggg tggcggccaa accgaccacg ttcgtctcga
     4081 cgatcttcct cagcttcgcc acgtcgccga tctcggcgcc gtcggtcccg ctcgcgatcc
     4141 cggcgttcgc gatcacgata tcaggcggcc cgtagcgttg gatgaagtcc tgcgctgccg
     4201 tggtcagcgc gcgctcattg ttcacgtcga gcgcgtagga gactacctcg ccaggcagcg
     4261 cgggatcgac cctgcggcgg gaaatcagtc cgaggactac gccttcgcgg gcatagcgcc
     4321 gcgccagcgc ttctccgata cccgaggagg cgccggtgac aatgacccgc acgtggtcgt
     4381 tcttggttcc ctatttctgc ttgcggacct gcgtcacgag ctggttgacg accgcgagca
     4441 tcgcctcgct cgagggcacg gtatagcggc cctgcaccgc catggcgggc gtgccgtcga
     4501 tcttgtagtc ggtggtgagg cggatggcgc gcttggtctt gctttgcacg ccgaaggatt
     4561 tcgccgtcgt ttcgaacttc ttcgggtcga ccccgtgctt ctcgagccac gcggaaagct
     4621 gcttggagtc atcggtgcgc aggcggtcgc gatggatggc gtcgaagagc ggccggtgca
     4681 gcttgtccac cagcccgagc gcttcgaagg cgtaatagat agtggcatcg tgtgcccagc
     4741 ggtcgttgaa gacggccggc acgcgcctga actccacgtc cttcggcagc gtcttgagcc
     4801 aactctcgag cttcggctcg aggttgtagc agtgaatgca cccgtaccag aagaattcga
     4861 ggacctcgat cttgtcggtt gtctcgaccg gctgcggggg gttgagctcg tagtaatccg
     4921 ggcttcgctg gccgagcgcg gcgagcgggg ccagggaaag cgtcgcgaca agcgc
//
LOCUS       DASUNT010000661         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_872463, whole genome shotgun sequence.
ACCESSION   DASUNT010000661 DASUNT010000000
VERSION     DASUNT010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872463"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VFX94_13250"
     CDS             <1..538
                     /locus_tag="VFX94_13250"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5769194.1"
                     /translation="AIEHAQRAYRRDNLAFQVGDAMALNFADNAFDVVVCSHVYEHVP
                     DSRRMMVEILRVLKPGAVCYFAAANRLVWREPHYGLPLLSVLPRSLAHGYMRLAGKGR
                     YYHELHLTYWGLRKLVERFQLHDYTRRILSDPEAFGAAYMLKRGSPKARLATAIARHA
                     YWLLPSYIWLLRKPQASA"
     gene            570..1265
                     /locus_tag="VFX94_13255"
     CDS             570..1265
                     /locus_tag="VFX94_13255"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5769195.1"
                     /translation="MRRAYEAAYGHIARCSGPEAAILDCGANNGWAFDVLAERFGITR
                     AQYHGIEWNARCAEQGRARGLDIAQGDLNQGIACDDGSFSCIQALSVLEHLLNPCRFL
                     KACHRVLRPGGRLVLLTPNISTYFTAVLILLGRMPSSGPHPDSNELLERAEIFRVSGA
                     DRQPDTEDDTPMHRHLIVFSYRVLHDYLRAIGFSQVHGRGFGLYPFPVFMQPVLERLD
                     PYHCHQMVFVAKK"
     gene            complement(1273..>2384)
                     /locus_tag="VFX94_13260"
     CDS             complement(1273..>2384)
                     /locus_tag="VFX94_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769196.1"
                     /translation="GGIGILRDDAGNDTYEAQMFAQGTGYYYGIGLAWDLAGDDQWRA
                     VRYAQGNGVHEAVGVLRDEAGDDAYELSYGVGQGMGLDLGLGVLFDAAGDDRYQATVH
                     AQGNATSNGIGLLFDRAGADEWRMSADPRAWGRAEPARRLPSLGLLLYEPGRAAFFRE
                     SKPVAPPNDGAPIDVPEPSTERTCPPAADVPPSEEASFAHALRSLEFLFRGRPADPAT
                     QAYVRQRLVNDLQDALAELPRDDFTTEWILGNFLPCVLREATPDEAGEMWDAMERTLV
                     SDLESPFAIAMALALRERPAPPVQNARITQALARHRSCQVRGAALVLDSSAPAAQAAL
                     RSSCWFLQATALGILAKLGAGIDPGTPLPSFLRVP"
BASE COUNT          402 a          785 c          755 g          442 t
ORIGIN      
        1 ggcaatcgag catgcccaac gcgcttaccg gcgcgacaac ctcgccttcc aggttggcga
       61 cgcgatggcg ctcaatttcg cggataacgc gttcgatgtc gtggtctgtt cgcacgtcta
      121 tgagcatgtt ccggattcgc gcaggatgat ggtcgagata ctccgcgtcc tgaaacccgg
      181 ggcagtgtgc tacttcgccg cagccaaccg cctcgtgtgg agggagccgc attacggact
      241 gccgttgctc tccgtattgc cgcgcagcct ggcgcatggg tacatgcgcc tagcgggaaa
      301 gggacggtac tatcacgagc tgcatctaac ttactggggc ttgcgcaagc tggtcgagcg
      361 cttccagttg cacgattaca cccgaaggat cctgtccgac cccgaggcgt tcggcgccgc
      421 ttacatgctg aagagaggta gtcccaaagc tcgcctcgct acggccattg cccggcatgc
      481 ctattggctc ctgccgagct atatctggct gctgcgcaag ccgcaagcaa gtgcttagca
      541 agtacttcaa ggccctgtac cgagatgcca tgcgccgtgc atatgaggcg gcctacggcc
      601 acatcgcgcg ctgttccgga cccgaggcgg cgattctcga ctgcggggcg aataacggct
      661 gggcgtttga cgtcctggcc gagcgcttcg gcatcacgcg agcgcagtat cacggcatcg
      721 aatggaacgc gcgctgcgcc gagcaaggac gcgcgcgcgg gcttgatatc gcgcagggcg
      781 atctcaatca gggcatcgcg tgcgacgacg gcagtttttc gtgcatccag gcgctttccg
      841 tcctcgagca cttgcttaat ccgtgtcgat ttctgaaggc ctgccatcgg gtcctgcggc
      901 cgggcggccg cctcgtgcta ctcacgccca atatcagcac ttacttcacg gcggttctta
      961 tcctgctcgg caggatgccc tcgagcggcc cgcatcccga ttccaatgag ctcctggagc
     1021 gcgcggaaat cttcagagtg agcggcgcgg atcggcagcc cgacacggag gacgatacgc
     1081 cgatgcatcg ccacctgatc gttttcagct accgcgtgct gcacgattac ctgcgcgcga
     1141 ttggtttctc gcaggtgcac ggccgcggtt tcggcctgta ccccttcccg gtcttcatgc
     1201 agccggtcct cgagcggctc gatccgtacc attgtcacca gatggtcttc gtcgcaaaga
     1261 agtgaccacc gtctatggga cgcgcagaaa ggacggcaag ggcgtcccag gatcgatgcc
     1321 ggcgccgagc ttggccagta tgccgagcgc cgtggcttgc agaaaccagc acgacgagcg
     1381 cagcgccgcc tgcgcggccg gcgctgagct atcgagcacc agcgcagcgc cccgcacttg
     1441 gcagctccgg tggcgagcca gggcttgagt gattcgcgcg ttttgcaccg gcggagcggg
     1501 gcgctcgcgc aacgccagcg ccatggcgat cgcgaaaggg ctctccagat cggagacgag
     1561 cgtcctttcc atggcatccc acatctcgcc agcttcatcc ggcgttgctt cccgtaacac
     1621 gcatggcaga aagttgccga gtatccattc ggtggtgaaa tcgtcgcgcg gaagctccgc
     1681 aagcgcgtct tgcaggtcgt tgacgagccg ttgtcgcaca taggcctgcg ttgccggatc
     1741 agccgggcgc ccgcgaaaaa ggaattccag gctccgcagg gcatgcgcga acgatgcttc
     1801 ctcgctgggc ggcacgtcgg ccgccggcgg gcaagttcgc tccgtacttg gctcgggcac
     1861 gtcgatcggc gcgccatcgt tgggtggcgc taccggcttg ctttcgcgga agaacgccgc
     1921 tctgccgggt tcgtagagta gaagcccgag gctgggcagt ctgcgcgccg gttccgcccg
     1981 gccccaggct cgcgggtcgg cgctcatgcg ccactcgtcg gcgcccgcgc ggtcgaaaag
     2041 cagcccgatg ccattcgacg tggcgttgcc ctgcgcgtga acggtggcct gatagcggtc
     2101 atcgccagcg gcatcgaaga gcacgcccag gccgaggtca agacccatgc cctggccaac
     2161 gccgtaagaa agctcgtagg catcgtcgcc ggcttcgtcc cgcagcacac cgaccgcttc
     2221 gtgcacccca ttaccttggg cgtatcgcac ggcgcgccac tggtcgtcgc ccgcgagatc
     2281 ccaggcgagc ccgatgccgt agtagtagcc cgtaccctgc gcgaacatct gcgcctcgta
     2341 cgtgtcgttg cctgcgtcgt cgcgcaggat tccaatgccg ccag
//
LOCUS       DASUNT010000662         6331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_259804, whole genome shotgun sequence.
ACCESSION   DASUNT010000662 DASUNT010000000
VERSION     DASUNT010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259804"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VFX94_13265"
     CDS             complement(<1..718)
                     /locus_tag="VFX94_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HEX5769197.1"
                     /translation="MKHARIVVTRYGGPDELRLVEEDRPEPKDGEVRVKMLAAGVALP
                     DLMMREGIHPETPRVPFTPGWDLVGLVDRLGEGVSGIEPEQTVAALPIAGAYAEYVCV
                     PQQELVPVPAGVDPAEAVSLILNYITAYQMIHRSAKVRAGQRALIHGAAGGVGTALLQ
                     LGRLAGLEMYATCSSRGAATVSELGAIPIDYRQLDFVEEIHRLTGDGVDAVFDGIGGS
                     HIWRSRKALRRGGKVVAYGLT"
     gene            complement(715..1470)
                     /locus_tag="VFX94_13270"
     CDS             complement(715..1470)
                     /locus_tag="VFX94_13270"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5769198.1"
                     /translation="MSKLAVGGVSKSFAQTTALEKIDLAIADNDFLCILGPSGCGKST
                     LLRIVAGLETPSTGRVLLDGQAVERPGPDRGMVFQSYTLFPWLTVRQNILFGARSTAA
                     VADSLIVKVGLKGFENHYPKQLSGGMQQRTALARALANNPKILLLDEPFGALDNQTRA
                     LMQELLLGIWEADRKTVLFVTHDIEEAIFMANRVAVMSARPGHIKSIVPIALPHPRHY
                     TMKTTPEFSRYKAELTEEIRVESIRTMQMASAA"
     gene            complement(1467..2240)
                     /locus_tag="VFX94_13275"
     CDS             complement(1467..2240)
                     /locus_tag="VFX94_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5769199.1"
                     /translation="MRPLRPVGPLARVALGAAFFVLFFAAWALATFGGYVSPTFLADP
                     ASMVREGWNLLVHHGFIKDILITIWRVFGGFLLAAAIGIPLGMAMGAYKPIEAFFEPF
                     ISFSRYLPASAFIPLLILWAGTGEAQKLLVIFVGSFFQIVLMVAVTVGNTRRDLVEAA
                     YTLGASDRAIVTRVLFPSAAPEIAEISRLVLGWAWTYVIVAELIGASSGIGHMIMDSQ
                     ALLNTGQIIFGIVVIGVIGLVSDYLFKTLNRRLFPWSLA"
     gene            complement(2271..3206)
                     /locus_tag="VFX94_13280"
     CDS             complement(2271..3206)
                     /locus_tag="VFX94_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5769200.1"
                     /translation="MKRLVVISVVALAAQPAFSQDKVNIGISGWTGFAPLTLALQAGI
                     FKQNGVEATIKKIPQQSRHLAIASGDIQCAATTVETWLVWNANGVLTKQIFQLDKSYG
                     ADGMATRANVAKIADLKGKTIGSQAPGTSPYFVMAWMLKKNGLTPKDVTVVSIDPGPA
                     AQAFLAGQYDAVMTYEPYLSSIRAKPDIGKIIATTLDYPVVMDTFGCTPKYLAENGRA
                     ARAMTQSYFQALEMIAKEPQKSYEIMGADVKQSAEQFGNSAKYLRWQDRAANQKFFGG
                     ELQAFLKEAGQILLEIGIIKSMPDVNSTIDTSYIK"
     gene            3245..4909
                     /gene="hutU"
                     /locus_tag="VFX94_13285"
     CDS             3245..4909
                     /gene="hutU"
                     /locus_tag="VFX94_13285"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX5769201.1"
                     /translation="MNSPKSRVVRSPRGSKLSAKSWLTEAPLRMLMNNLDPEVAERPA
                     DLVVYGGIGKAARNWECYEAIVESLVSLEDDQSLLVQSGKPVGVLRTHPDAPRVLIAN
                     SNLVPNWASWEHFNELDRKGLMMYGQMTAGSWIYIGSQGIVQGTYETFAEAGRKHYGG
                     DWSGKWILTAGLGGMGGAQPLAATMAGASMLAVECDPSRIEMRLKSGYLDKRAASLDE
                     AINILSEAAAPVSVALLGNAAEVVPQILKKAKASRFRPALVTDQTSAHDPANGYLPEH
                     WSLEKWKDLRKREPDEVAMSARESIANHVQAILQFKAMRIPCVDYGNNIRQEAKNAGV
                     KNAFDYPGFVPAYIRPMFCVGKGPFRWVALSGDPQDIERTDAKVRELFPDDPGLQRWL
                     DLASEKIRFQGLPARICWLGLGQRHRAGLAFNEMVRTGELKAPIVIGRDHLDAGSVAS
                     PNRETEGMRDGTDAVSDWPLLNLMLNTASGATWVSFHHGGGVGMGYSQHAGMVIVCDG
                     TEAAAKRIERVLWNDPASGVLRHADAGYAEAIACAQAKGLAVPMRR"
     gene            4906..>6331
                     /gene="hutH"
                     /locus_tag="VFX94_13290"
     CDS             4906..>6331
                     /gene="hutH"
                     /locus_tag="VFX94_13290"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX5769202.1"
                     /translation="MKIGARPFDLPALRRIAREGPQLELDPTCLPRLRAGLESVERIV
                     ASGKPAYGINTGFGRLSQTRIPDEELGQLQTNIVLSHAAGTGALLDDATVRLVLALKV
                     LSLSTGYSGVRPLLVERLLQLYNRGIYPCIPAKGSVGASGDLAPLAHLSLALLGIGDV
                     RVNGKIVAARQALDDLEPLRLAPKEGLALLNGTQVSSALALIGLFAAEDVFAAALAAG
                     GLSVDAAAGSDTPFDSRIHALRGQAGQQLVARQYAELLAQSEIRRSHLENDPRVQDPY
                     SLRCQPQVMGACLDQMRHAARIFEIESAAVTDNPIVFFETDEILSGGNFHAEPVALAA
                     DVLALAIAEIGALSERRIALLIDSSLSGLPPFLVRHGGVNSGFMVAQVTAAALAAENK
                     SLAHPASVDSLPTSANQEDHVSMATFAARRLSEMARNSAGIVAIELLSAAQGIDLRAP
                     LKTSPRLQEVHSLIRSRVGFYDHDR"
BASE COUNT         1108 a         2091 c         2076 g         1056 t
ORIGIN      
        1 cggtgaggcc ataggccacg acctttccgc cacgacggag agccttgcgg gaacgccaga
       61 tgtggctgcc gccgatgccg tcgaagacgg cgtctacgcc gtcgcccgta aggcggtgaa
      121 tttcttcgac gaagtcgagc tgtcggtagt caatcgggat agcacccagc tccgatacgg
      181 tcgccgcccc tcgcgatgag caagtcgcgt acatctccaa tcccgcaaga cgcccaagct
      241 gcagcagtgc cgtgccgacc ccgcctgcgg cgccgtgaat caacgcgcgc tggccggctc
      301 tcaccttggc ggagcggtgg atcatctggt acgcggtgat gtagttgagg atcaggctga
      361 cggcctctgc gggatccacg ccagccggca ctgggaccag ctcctgctgc ggcacgcaga
      421 catactcggc gtacgcgccg gcgatcggta gcgcggcgac cgtctgctcc ggctcgatgc
      481 cagaaacgcc ctcgccgaga cgatccacca ggccgaccag atcccagccg ggagtgaagg
      541 gcacgcgcgg tgtctcggga tgaatcccct cgcgcatcat caggtcgggc aaggcgacac
      601 ctgccgcaag catcttcacc cgtacttcgc catcttttgg ctcggggcgg tcttcttcaa
      661 ccagccgaag ttcatcgggc ccgccgtagc gggtgacgac gatgcgagcg tgtttcacgc
      721 cgcagacgcc atttgcatcg tgcggatcga ctcgacgcgg atctcttcgg tcagctcggc
      781 cttgtagcgt gagaactccg gcgtggtctt catcgtgtag tgccgcgggt gcggcagcgc
      841 gatcggcacg atggacttga tgtgtccggg ccgcgccgac atcaccgcca cgcggttcgc
      901 catgaagatc gcctcctcga tgtcgtgggt gacgaagagc acggttttgc ggtcggcctc
      961 ccagatgccg agcagcagct cctgcatgag cgcccttgtc tggttgtcga gcgcgccgaa
     1021 gggttcgtcc agcaacagga ttttcgggtt gttggccagt gcgcgggcga gcgcggtgcg
     1081 ctgctgcatg ccgccggaaa gttgcttcgg gtagtggttc tcgaagcctt tgagcccgac
     1141 tttgacgatg agtgaatctg ccaccgctgc cgtcgagcgc gcaccgaaaa ggatgttctg
     1201 ccgtacggtg agccacggga agagcgtgta ggactggaac accatgccgc ggtcgggccc
     1261 cgggcgctcg accgcctggc catcgagcaa cacgcgcccg gtgctcggcg tttccagacc
     1321 ggccacgatg cgcaaaaggg tcgacttgcc gcagcccgag gggccgagga tgcaaaggaa
     1381 gtcgttatcc gcgatggcga gatcgatttt ctccaaggcc gtggtctggg caaagctctt
     1441 cgatactccc ccgaccgcca gcttgctcac gcgaggctcc agggaaagag ccgccggttg
     1501 agcgtcttga agaggtaatc cgaaacgagg ccgatcacgc cgatcaccac gatgccgaaa
     1561 atgatctgcc cggtattaag cagcgcctgg ctgtccatga tcatgtggcc gatgccggaa
     1621 gaagcgccga tcagctcggc gacgatcacg tacgtccacg cccagccgag cacgaggcgc
     1681 gaaatctccg cgatctcggg cgcagccgac gggaaaagca cgcgggtcac gattgcccga
     1741 tcgctcgcgc cgagcgtgta ggccgcttcc acaagatcgc gccgcgtatt gcccacggtg
     1801 actgccacca tgagcacgat ctggaagaac gagccaacaa agatgacgag gagcttctgc
     1861 gcctcgccgg ttccggccca caggatcagc agcggaatga acgccgaagc cggcaggtag
     1921 cgcgagaacg agatgaacgg ctcgaagaac gcctcgatcg gcttgtacgc gcccatcgcc
     1981 atcccgagcg ggataccgat cgccgcggcg aggagaaagc cgccgaacac ccgccagatg
     2041 gtgatgagga tgtccttgat gaagccgtga tgcacgagca ggttccagcc ctcgcgcacc
     2101 atacttgcgg gatcggcgag aaaggtcggc gacacatagc cgccgaacgt ggcgagcgcc
     2161 caggcggcga agaagaggac gaagaaagcc gcgccgagcg cgacccgcgc gagcggcccg
     2221 accggcctaa gcggcctcac gccgcgctag tttctacggg agtcaacggc ttacttgatg
     2281 tagctcgtgt cgatggtgct gttcacgtcg ggcatcgact tgatgatgcc gatctcgagc
     2341 aggatctgcc cggcttcctt gaggaaagcc tgcagctcgc cgccgaagaa cttctggttc
     2401 gccgcgcggt cctgccagcg caggtacttg gcggagttgc cgaactgctc ggcgctctgc
     2461 ttcacgtcgg cgcccatgat ctcgtaggac ttctgcggct cttttgcgat catctccagc
     2521 gcctggaaat agctctgcgt catcgcgcgc gccgcgcggc cgttttcggc caggtatttg
     2581 ggcgtgcagc cgaacgtgtc catcacgacc gggtagtcga gcgtggtggc gatgatcttg
     2641 cctatgtcgg gcttcgcgcg gatcgaggag aggtacggct cgtaggtcat taccgcgtcg
     2701 tactgaccgg cgaggaacgc ctgcgccgcc ggcccgggat cgatcgacac gaccgtgacg
     2761 tccttcggcg tgaggccgtt cttcttcagc atccaggcca tgacgaaata gggcgacgtc
     2821 cccggagcct gcgagccgat cgtcttgccc ttgaggtcgg cgattttcgc gacgttggcg
     2881 cgcgtcgcca tgccgtcggc gccgtagctc ttgtcgagct ggaatatttg cttcgtcagc
     2941 acgccgttcg cgttccacac gagccaggtc tcgaccgtgg tcgccgcgca ctggatgtcg
     3001 cccgaggcga tagcgaggtg gcggctctgc tgcgggatct tcttgatggt ggcttcgaca
     3061 ccgttctgct tgaagatacc tgcctgaagc gcgagcgtga gcggcgcgaa tcccgtccag
     3121 ccgctgatgc cgatattcac tttgtcctgg gaaaaagcgg gctgggcggc gagcgccacc
     3181 acggaaatga cgacaagacg tttcatcggt tctccccggg ttctgtgcaa agatgttact
     3241 ccaaatgaac tccccgaaat cccgcgtcgt gcgctcgccg cgcggcagca agctgagcgc
     3301 gaagagctgg ctcaccgagg cgccgctgcg catgctgatg aacaacctcg accccgaggt
     3361 ggccgagcgg cccgcggacc tggtggtgta cggcggcatc ggcaaggcgg cccgcaactg
     3421 ggaatgctac gaagcaatag tagaaagtct tgtttctctg gaagatgatc agagcctgct
     3481 cgttcaatcc ggaaagcccg tcggcgtgct taggacgcac cccgacgccc cgcgggtgct
     3541 gatcgcgaac tccaacctcg tgccgaactg ggcgagctgg gagcacttca acgagctcga
     3601 tcgcaagggc ctgatgatgt acgggcagat gacggcaggc tcgtggatct acatcggcag
     3661 tcagggcatc gtgcagggaa cctacgaaac cttcgccgaa gcgggccgca agcactatgg
     3721 cggcgactgg agcggcaaat ggatcctcac cgcgggcctc ggcggcatgg gaggcgccca
     3781 gccgctcgcc gcaaccatgg cgggcgcttc gatgctcgcc gtcgagtgcg atccttcgcg
     3841 catcgagatg cggctgaagt cggggtacct ggacaagcgc gcagccagcc tcgacgaagc
     3901 catcaacata ttgagcgagg ctgcagcgcc ggtttcagtg gcgctgctcg gcaacgcggc
     3961 cgaagtggtt ccccaaatcc tgaagaaagc aaaagcctcg aggttcaggc ccgcgctcgt
     4021 gaccgaccag acgtcggccc acgatccggc caacggctac ctgcccgagc actggtcgct
     4081 ggagaagtgg aaggacctta gaaagcgcga gcccgatgag gtggccatgt cggcgcgcga
     4141 gtcgatcgcc aaccatgttc aggcgatcct tcagttcaag gcgatgcgaa ttccctgcgt
     4201 cgactacggc aacaacatcc gccaggaagc taagaacgct ggcgtcaaaa acgccttcga
     4261 ctatccgggc ttcgtaccgg cgtacatccg gccgatgttc tgcgttggga aagggccgtt
     4321 ccgctgggtg gcgctctcgg gcgacccgca ggacatcgaa cggaccgacg ccaaggtgcg
     4381 cgagcttttt ccggatgacc cggggttgca gcgctggctc gaccttgctt cggaaaagat
     4441 caggttccag ggcctgccgg cgcgcatctg ctggctcggc ctcggacagc gccatcgcgc
     4501 cgggctcgcg ttcaacgaga tggtgcgtac gggcgagctc aaggcgccga tcgtaatcgg
     4561 gcgcgatcac ctcgacgcag gctcggtggc gagcccgaac cgcgagaccg aaggcatgcg
     4621 cgacggcacc gatgcggtct cggactggcc gctcctcaac ctgatgctca acaccgcttc
     4681 gggggcgacc tgggtgtcgt tccatcatgg cggcggcgtt ggcatgggct actcgcagca
     4741 cgccggcatg gtcatcgtgt gcgacggcac cgaggccgcg gcgaagcgca tcgagcgcgt
     4801 gctgtggaac gacccggcga gcggcgtgct gcgccacgcc gatgccggct acgccgaagc
     4861 catcgcctgc gcgcaagcga agggtctcgc cgtgccgatg cggcgatgaa gatcggcgcg
     4921 cggcccttcg accttcccgc cctgcgccgc atcgcgcggg aaggcccgca gctcgagctc
     4981 gacccgacgt gcctgccccg gctgcgggcg ggactcgaat cggtcgagcg catcgtggcg
     5041 tccggcaagc ccgcgtacgg catcaacacc ggatttggcc gcctgtcgca gacgcgcatc
     5101 ccggacgaag agctcgggca gctgcagacc aacatcgtcc tttcgcacgc cgccggcacc
     5161 ggagcgctcc tcgacgacgc gaccgtgcgc ctcgtgctcg cgctcaaggt cctgtctctc
     5221 tccaccggct actcgggcgt gcggccgctc ctcgtcgagc gcctgctgca actctacaac
     5281 cgcggcatct atccctgcat cccggcgaag ggctcggtcg gcgcctcggg cgacctcgcg
     5341 cccctcgccc acctgtccct tgcgctcctc gggatcggcg atgtccgtgt aaacggaaag
     5401 atcgtcgccg cgcgccaggc attggacgac ctggagccgc tcaggctcgc gcccaaggaa
     5461 gggctggcgc ttctcaacgg cacccaggtt tcgagcgcgc tcgcgctgat cggcctcttc
     5521 gcggccgagg acgtattcgc cgcggcgctg gccgcggggg ggctctccgt ggacgcggcc
     5581 gcggggagcg acacgccgtt cgactcgcgc atccacgcgc tgcgcggcca ggccgggcag
     5641 cagctcgtgg cgcgtcagta cgccgagctc ctcgcccaga gcgagatccg ccgctcgcac
     5701 ctcgagaacg acccgcgcgt gcaggacccc tacagcctgc gctgccagcc gcaggtgatg
     5761 ggcgcgtgcc tcgaccagat gcgccacgcg gcgcgcattt tcgagatcga atcggccgcg
     5821 gtcacggaca acccgatcgt gttcttcgag acagacgaga tactgtcggg cgggaatttc
     5881 cacgccgagc cggttgcgct cgcggccgat gtgctcgcgc tcgcgatcgc cgagatcggc
     5941 gcgctctccg agcggcgcat cgcgctcctc atcgacagct cgctatccgg cctgccgccg
     6001 ttcctcgtcc ggcacggcgg cgtgaattcg gggttcatgg tggcgcaggt caccgcggcc
     6061 gcgctcgcgg cggaaaacaa gtcgctcgca cacccggcga gcgtggacag cctgccgacg
     6121 tcggcgaacc aggaggacca cgtcagcatg gcgaccttcg cggcccgaag gctttccgaa
     6181 atggcgcgta acagcgccgg catcgtcgcc atcgagctcc tttccgcggc gcagggcatc
     6241 gacttgcgcg cccccctcaa gacctcgccg cgcctgcagg aagtgcattc cctcatccgc
     6301 tcgcgagtcg gcttctacga ccacgaccgc t
//
LOCUS       DASUNT010000663         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_435832, whole genome shotgun sequence.
ACCESSION   DASUNT010000663 DASUNT010000000
VERSION     DASUNT010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435832"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /gene="accA"
                     /locus_tag="VFX94_13295"
     CDS             complement(<1..609)
                     /gene="accA"
                     /locus_tag="VFX94_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEX5769203.1"
                     /translation="MKTTFLEFEQPIAELETRIEELRFVQDDSAVDISEEIQRLTKRS
                     QSLTKDIYGKLTPWQVAQVARHPQRPFTLDYIQALFTHFEELHGDRTFSDDASIVGGL
                     ARFNGAPCVVLGHQKGRDTKEKIARNFGMPKPEGYRKALRLMKLAEKFGLPVFSFVDT
                     PGAYPGIDAEERGQSEAIGRNLFEMARLRAPIIVSIIGEGGSG"
     gene            complement(617..1234)
                     /locus_tag="VFX94_13300"
     CDS             complement(617..1234)
                     /locus_tag="VFX94_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX5769204.1"
                     /translation="MKPEYWDRAKRALARGDPVMAAIMRSHPKVFLMRRGEPFLTLAR
                     AICGQQISVKAAQSVWDRVVACCGEVTPEKVLSRPRPQLRACGLSDRKTEYIADLAQH
                     FADGRIHLHRWPEMSDDEIIAELVEVRGIGRWTAEMFLIFNLLRPDVFPLDDLGLQKG
                     IRVSYFKGRQVSLARMRKLGESWRPWRSVATWYLWRSLDPLPVEY"
     gene            complement(1224..>2264)
                     /locus_tag="VFX94_13305"
     CDS             complement(1224..>2264)
                     /locus_tag="VFX94_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6352 family protein"
                     /protein_id="HEX5769205.1"
                     /translation="STWRRTRAWSSHLPEFWRNSGFRLLERDPAGKLRVTDDFLRAYY
                     LRSELHPVEESGVAERRLHASLMESPARNIAEAEIQAVEDADARDNYRILLRFRQKLL
                     DTGTLEGSYMSLFRAPIDVPPMFVDQLAHVILRNILDGCDDPLRLRAAELFFREQKAT
                     IQDGHVLLADLETVEMHASGNRYGSIGRLIVEAQGDLAKVDLDVLDRANAAIYWERES
                     RHDTVISLTYGRPALDALCRVMETWIAHFLALRVSIKPLRKIDEPRWAWHVGLDAEST
                     AILNELWSGGEIEPGRMRNLLAIFALDFDEQNAMRADVRGRTVYLALSCEDGVVKMKP
                     QNLLTNLPLNEA"
BASE COUNT          377 a          741 c          731 g          415 t
ORIGIN      
        1 gcccgagccg ccctcgccga tgatggacac gatgatcggc gcgcgcaggc gcgccatctc
       61 gaacaggttc ctgccgatcg cctcggattg gccgcgctcc tctgcgtcaa tcccgggata
      121 ggcgcccggc gtgtcgacga aactgaacac cggcaggccg aacttctctg cgagcttcat
      181 gagccgcagc gccttgcggt agccctctgg cttgggcatg ccgaagttgc gcgcgatctt
      241 ctccttggtg tcgcgcccct tctggtggcc gagcactacg cacggcgcgc cgttgaagcg
      301 cgcgaggccg ccgacgatcg aggcgtcgtc ggaaaaggtg cggtcgccgt gcagctcctc
      361 gaagtgcgtg aacagtgcct ggatgtagtc gagcgtgaac ggccgctgcg ggtggcgcgc
      421 cacctgcgcc acctgccacg gcgtcagctt gccgtagatg tccttggtga gcgactggct
      481 gcgcttggtg aggcgctgga tctcctccga gatgtcgacg gccgaatcgt cctgcacgaa
      541 gcgcagctct tcgatgcgcg tctcgagctc cgcgatcggc tgctcgaatt cgaggaacgt
      601 ggtcttcatc cccggctcag tattctaccg ggagaggatc gagcgagcgc cacaggtacc
      661 aggtggccac cgagcgccac ggccgccagc tttcgccaag cttgcgcatg cgcgccagcg
      721 aaacctgtct ccccttgaag taacttactc gtatgccctt ctgcagcccg aggtcgtcga
      781 gcggaaatac atctggccgc agcaggttga agatgaggaa catctcggcg gtccagcgcc
      841 cgatgccgcg cacctcgacc agctcggcaa tgatctcgtc gtccgacatc tcgggccagc
      901 gatgcaggtg gatgcggccg tcggcgaagt gctgcgccag gtcggcgatg tattccgtct
      961 tgcgatccga caacccgcaa gcacgcagtt gcggtctggg tcttgaaagc accttctcag
     1021 gagtcacttc cccgcagcaa gcgacgacgc ggtcccagac gctttgcgcg gccttgaccg
     1081 agatctgctg cccgcagatc gcgcgcgcaa gcgtgaggaa cggctccccg cggcgcatga
     1141 ggaacacctt cgggtgcgaa cgcatgatcg cggccatcac cgggtcccct cttgccagcg
     1201 cacgcttcgc gcggtcccag tattcaggct tcattgagag gcaggttggt caacaggttc
     1261 tgcggcttca tcttcactac cccgtcttcg cacgagagcg cgagatagac cgtcctgcca
     1321 cgaacgtcgg cgcgcattgc gttctgctcg tcgaaatcga gcgcgaagat cgccagcaga
     1381 ttgcgcatcc gccccggctc gatctcgcct ccgctccaga gctcgttcag gatggcggtg
     1441 gactcggcgt cgagccctac gtgccaagcc cagcgcggct cgtcgatctt cctcagcggc
     1501 ttgatggaaa cgcgcagcgc aaggaagtgc gcaatccacg tttccattac gcggcagagc
     1561 gcatcgaggg cgggccgacc gtaggtcagg ctgattacgg tgtcgtggcg cgactcgcgc
     1621 tcccagtaga tcgccgcgtt ggcgcggtcg agcacgtcga ggtccacctt ggccaggtca
     1681 ccttgcgcct cgacgatgag ccgtccgatc gatccgtagc ggttgccgct cgcgtgcatc
     1741 tcgaccgttt caaggtccgc gagcagcacg tggccgtcct gtatggttgc cttctgctcg
     1801 cggaaaaaaa gctccgccgc gcgcagccgc agcggatcgt cgcagccgtc gaggatgttg
     1861 cgcaggatca cgtgcgcgag ctggtcgacg aacatgggtg gcacgtcgat cggcgctctg
     1921 aacaggctca tgtaggagcc ctcgagcgtg ccggtgtcga gcagcttctg ccggaagcgc
     1981 aggaggatgc ggtaattgtc ccgcgcgtcg gcgtcctcga ccgcctggat ttctgcttcc
     2041 gcgatgttcc ttgctggact ctccatcagc gacgcatgca ggcgccgctc ggccacgccc
     2101 gattcctcga ccgggtgaag ctccgagcgc agatagtagg cgcgcaggaa gtcgtcggtg
     2161 acccgaagtt ttccggccgg gtcgcgttct aggagacgaa agccgctatt gcgccagaac
     2221 tcaggcaggt gcgaggacca ggcgcgagtg cgacgccacg tgct
//
LOCUS       DASUNT010000664         2579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_259942, whole genome shotgun sequence.
ACCESSION   DASUNT010000664 DASUNT010000000
VERSION     DASUNT010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259942"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VFX94_13310"
     CDS             <1..655
                     /locus_tag="VFX94_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5769206.1"
                     /translation="GAVILRTRALTLLMLTLAITAILLEIANKWTSMTGGADGLSGVK
                     VGEILGLFRFDLYGRAAFFYCLVMLFIGWWVVRRIVHSPYGAMLTGIRENAARMDAIG
                     APVYWRLVLIYTISATLAGIAGALLTQVNQFVGLNVLGLEPSGDILVMLILGGVGRLY
                     GAFIGPVVYLIAQDYLAKQYPEYWYFGIGVLLIVVVLFARGGILGILDVFLRNKKAV"
     gene            624..1373
                     /locus_tag="VFX94_13315"
     CDS             624..1373
                     /locus_tag="VFX94_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5769207.1"
                     /translation="MYSLETRKLCKSFGALTVADNIDFRLEAGARHALIGPNGAGKTT
                     FVNMLMGALAPSSGHIFLGGEDVTRTPQSTRVRRGLGRTFQVTSLFRNLPVLDNVALG
                     IAERDGTATRMWRPASAHREIIEESRSLLAGLGLADDAGTRVADLAYGKQRLVEIAIA
                     LGLRPKVLLLDEPAAGVPSHESEIILTNLEKLPAEIAILIIEHDMELVFRFARRITVL
                     VQGTVLVEGPPQDIARDPRVHQVYLGEQGHA"
     gene            1366..2058
                     /locus_tag="VFX94_13320"
     CDS             1366..2058
                     /locus_tag="VFX94_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5769208.1"
                     /translation="MLSLAQVRAGYGDTVVLEDVDVELPERGALAVLGRNGVGKTTLL
                     ATIMGHTTFHSGRMTFKGAPIAHRPVYERSKLGIGYVPQEREIFPSLSLEENLLVAER
                     PGRWNLAAVYKLFPRLEERKAARGNQLSGGEQQMLAIGRALMGNPSLLLMDEPLEGLA
                     PIIVEALLISLKRLVSDDSLALVLVEQHAKLALQVTENAIVLNRGRVSYSGRSAELLA
                     DPQRLAALVLTQ"
     gene            complement(1985..>2579)
                     /locus_tag="VFX94_13325"
     CDS             complement(1985..>2579)
                     /locus_tag="VFX94_13325"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5769209.1"
                     /translation="GARARRARVVPAACARAPRPQEAASGGVTRFRPARVGDVDAIVG
                     MMRGCYGEDSYTFVEDEARAAALMLIEVQGLGRLWVISDGDTVVGYVAVALGFSLEYR
                     GREAFINELFIAESHRGRGLGGEALEIAEAWCREAGVNALHLEVERHRENALELYRRR
                     GFADHGRYLMTKWLTESRPGPRAAADRPAARRSARSS"
BASE COUNT          387 a          902 c          856 g          434 t
ORIGIN      
        1 cggcgcggtg atcctgcgca cccgggcgct cacgctcctc atgctgacgc tcgcgatcac
       61 ggcgatcctg ctcgagatcg ccaacaagtg gaccagcatg acgggcggcg cggacggcct
      121 gtccggggtc aaggtgggcg agattctcgg gctgttccgg ttcgacctct acggcagggc
      181 cgcctttttc tattgcctcg tgatgctgtt catcggctgg tgggtggtgc gccgcatcgt
      241 ccattcgccc tacggcgcga tgctcaccgg catccgcgag aacgcggccc gcatggacgc
      301 catcggcgcg ccggtgtact ggcggctcgt gctcatctac acgatctcgg ccacgctggc
      361 gggtatcgcg ggtgcgctgc tcacgcaggt gaaccagttc gtcgggctca acgtgctcgg
      421 cctcgagccc tccggcgata tcctggtcat gctgattctc ggcggcgtcg gccggctcta
      481 cggcgcgttc atcggtccgg tcgtctacct gatcgcccag gactacctcg ccaagcaata
      541 ccccgaatac tggtacttcg gcatcggcgt gctgctgatc gtcgtggtgc ttttcgcccg
      601 cggcggcatc ctcggaatcc tcgatgtatt ccttagaaac aagaaagctg tgtaagagct
      661 tcggtgcgct gaccgtcgcc gacaacatcg acttccggct cgaggcgggg gcgcgccacg
      721 cgctcatcgg gccgaacggc gcgggcaaga ccactttcgt gaacatgctg atgggcgcgc
      781 tcgcgccgtc ctccggtcac atcttcctcg gcggggagga cgtgacgcgc acgccgcagt
      841 cgacgcgcgt gcgccgtggc ctcgggcgca cgttccaggt cacgtcgctt ttccgcaatt
      901 tgcccgtgct cgacaatgtc gcgctcggca tcgcagagcg cgatggcacc gcgacgcgca
      961 tgtggcgccc cgcttcggcg catcgggaaa tcatcgagga gagccgcagc ctgctcgccg
     1021 gactcgggct cgccgacgat gccggcaccc gcgttgccga cctcgcctac ggcaagcagc
     1081 gcctcgtcga gatcgcgatc gcgctcggct tgcggccgaa ggtgctgctg ctggacgagc
     1141 cggccgccgg cgtgccctcc cacgagtccg aaatcatcct gacgaacctg gaaaagctgc
     1201 ccgcagagat cgccatcctc atcatcgagc acgacatgga gctcgtcttc cgcttcgcgc
     1261 gccgcatcac ggtgctggtc cagggcacgg tgctggtgga agggccgccg caggacatcg
     1321 cgcgggaccc gcgcgttcac caggtgtacc tcggagagca gggacatgct tagcctcgcg
     1381 caggtacgcg cgggctacgg cgacacggtg gtgctcgagg acgtcgatgt ggagcttccc
     1441 gagcgcggcg cgctcgccgt gttggggcga aacggcgtcg gcaagaccac gcttctcgcc
     1501 accatcatgg gccacaccac ctttcactcg ggacgcatga ccttcaaggg cgctccgatc
     1561 gcgcaccggc cggtctacga gcgctcgaag ctcggcatcg gctacgtgcc gcaggagcgc
     1621 gagatcttcc cctcgctctc gctcgaagag aacctgctcg tcgccgagcg cccggggcgc
     1681 tggaacctgg ccgcggtcta caagctcttt ccgcggctcg aggagcgcaa ggccgcgcgc
     1741 ggcaaccagc tctccggcgg cgagcagcag atgctcgcga tcgggcgcgc acttatgggc
     1801 aatccctcgc tcctcctcat ggacgagccg ctcgaggggc tcgcgccgat catcgtcgag
     1861 gcgctgctga tctccctgaa gcggctggtg tccgacgatt cgctggcgct cgtgctggtc
     1921 gagcagcacg ccaagctcgc gctccaggtg accgagaatg cgatcgtgct gaaccgcggg
     1981 cgcgtcagct actccgggcg gagcgccgag ctgctggccg atccgcagcg gctcgcggcc
     2041 ctggtcttga ctcagtaagc cacttggtca tgaggtagcg gccgtggtcg gcgaagccgc
     2101 ggcgcctgta gagctcgagc gcgttctcgc ggtggcgctc gacctcgagg tgcagcgcgt
     2161 tcacgcccgc ctcgcggcac caggcttcgg cgatctcgag agcctcgccg ccgagcccgc
     2221 gcccgcggtg gctctcggcg atgaagagct cgttgatgaa cgcctcgcgg ccgcgatatt
     2281 cgaggctgaa tccgagcgcc accgcgacgt agccaaccac cgtgtcgccg tctgagataa
     2341 cccaaaggcg gcccaggccc tgaacttcga tcaacatgag cgccgcggcc cgcgcctcgt
     2401 cctcgacgaa tgtgtagctg tcctcgccgt agcagccgcg catcatgccg acgatggcat
     2461 cgacatcgcc gacgcgcgcc ggccggaagc gggtcacgcc gccgctcgct gcctcttgcg
     2521 gtcggggcgc tcgcgcgcac gctgcaggaa ccacacgagc gcggcgagcg cgagcgccg
//
LOCUS       DASUNT010000665         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_959890, whole genome shotgun sequence.
ACCESSION   DASUNT010000665 DASUNT010000000
VERSION     DASUNT010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959890"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VFX94_13330"
     CDS             <1..359
                     /locus_tag="VFX94_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769210.1"
                     /translation="ANGLTSQPKDHAAALSMGSCALAAARRDPEHIEEERRVSAAFDR
                     IEKLCFRLLRGHRASTEALGSEWMRNYPGLDARIETNRGRVIFDLYLKLRYYDLGPLA
                     EWVNGGPAVRPAACAN"
     gene            complement(356..1090)
                     /locus_tag="VFX94_13335"
     CDS             complement(356..1090)
                     /locus_tag="VFX94_13335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5769211.1"
                     /translation="MSAPRPLAALSALDTERWRTVTAKASDVTGETLSPVLEFCRQNT
                     VRLLIARCAASELTGAQAMEAAGFQLMDTLVFYSRAVTDVLPDEAAALPIRTHKTGDE
                     AGVRAVAAQAFAGYQGHYHADPRLDQRQCDELYVDWAYRSCLSREIANAVLVAESDAR
                     IAAFATLRLNSAEEGEGVLFGVAPAAQGRGIYRSLMLAGMRWCKAQGCSRMVVSTQLT
                     NLAVQKVWSRLGFEPSSACYTFHKWF"
     gene            complement(1087..>2096)
                     /gene="rffA"
                     /locus_tag="VFX94_13340"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(1087..>2096)
                     /gene="rffA"
                     /locus_tag="VFX94_13340"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HEX5769212.1"
                     /translation="GCRKALLTHSCTAALEMAAILAGIGPGDEVIMPSFTFVSTANAF
                     VLRGAVPVFVDIRPDTLNIDETKIAAAITPRTRAIVPVHYSGVGCEMDAIMTIAAQHG
                     LLVIEDAAQGTLATYKNRALGAIGNLGALSFHETKNIISGEGGALLVNDTRLLERAEI
                     VREKGTNRSQFFRGQVDKYTWVDIGSSYLPGEIIAAFLWAQMEEAQQITARRLALWDR
                     YHAALADLECAGDARRPYVPPECKHNAHMYYLLLPSLEQRAAFIARLKGQEIQAVFHY
                     VPLHSSPMGRKLGRAAGELRHTEELSDRLVRLPFWLGLEERQAGVVQRVIDAARMKGA
                     P"
BASE COUNT          348 a          698 c          702 g          348 t
ORIGIN      
        1 tcgccaacgg cctgacttcg cagccgaagg accatgcggc ggctctgtcg atgggaagct
       61 gcgctcttgc ggcggccagg cgtgatccgg agcacatcga ggaggagcga cgcgtctcgg
      121 ccgcgtttga ccggatcgag aaactctgct ttcggctgct gcgcggccat cgcgcttcca
      181 ccgaggcgct tggctcggaa tggatgcgca actatccggg cctcgacgcc cgcatcgaaa
      241 cgaaccgcgg ccgggtgatc ttcgatctct acctgaagct gcgctactac gaccttgggc
      301 cgctagcgga gtgggtaaac ggcgggcctg ccgtgcggcc ggcggcctgc gcgaactaga
      361 accacttgtg gaaggtgtag caggcgctgg agggctcgaa ccctagcctg ctccaaactt
      421 tctgcacggc gaggttggtg agctgggtag aaacgaccat gcgcgagcag ccttgcgcct
      481 tgcaccagcg catcccggcg agcatgagag agcggtaaat gccgcgcccc tgcgccgcgg
      541 gcgccacacc gaacagcaca ccttcgcctt cctcggcgct gttcaggcgc agggtcgcga
      601 acgccgcaat tcgggcgtcg ctttcggcga ccagcaccgc attcgcgatt tcacgcgata
      661 ggcacgagcg gtacgcccag tccacataga gctcgtcgca ctgccgctga tcgagccgcg
      721 gatcggcgtg atagtgcccc tggtatccgg cgaaagcttg cgcggcgacg gcgcgcacac
      781 cggcttcgtc ccccgtcttg tgagtgcgaa tgggcagcgc ggccgcttcg tccggcagga
      841 cgtcggtcac ggcgcgcgag tagaagacca gcgtgtccat gagctggaag cccgcagctt
      901 ccatggcctg cgccccggtg agctcgctcg cggcacagcg tgcgatcagc agtctcacgg
      961 tgttctgccg gcagaactcg agcaccggag aaagcgtctc gcccgtgacg tcggacgcct
     1021 tcgccgtgac tgtcctccag cgctcggtat ctagcgcgga aagcgcggcg agagggcggg
     1081 gcgcgctcac ggtgcgcctt tcatgcgggc ggcgtcgatg accctctgca ccacaccggc
     1141 ctggcgctcc tccaggccga gccagaacgg cagccgcacc agccggtcgc tcagctcctc
     1201 cgtgtgacgc agctcgcccg ccgcgcgccc gagcttccgc cccataggcg agctgtgcag
     1261 cggaacgtag tggaataccg cctgaatctc ctgccccttc agccgcgcga tgaaggcggc
     1321 gcgctgctcg aggctcggta gcagcaggta atacatgtga gcgttgtgct tgcactccgg
     1381 cggcacgtag ggccggcgcg cgtcgccggc gcactcgagg tcggcaagcg ccgcatggta
     1441 gcgatcccac agcgccaggc gccgcgcggt gatctgctgc gcctcctcca tctgcgccca
     1501 caggaatgcg gcgatgatct cgcctggcag gtacgaagag ccgatgtcga cccaggtgta
     1561 cttgtccacc tgaccgcgga aaaactggct gcggttggtg cctttctcgc gcacgatctc
     1621 cgctcgctcg agcagccgcg tgtcgttcac gagcagcgcc ccgccttcgc cgctgatgat
     1681 gttcttcgtc tcgtgaaaag acagcgcgcc gaggttcccg atggcgccca gggcgcggtt
     1741 tttgtaggtg gcaagcgtcc cttgcgccgc atcctcaatg acgagcaagc cgtgctgggc
     1801 cgcgatggtc atgatcgcgt ccatctcgca gccgacaccg ctgtagtgca ccgggacgat
     1861 cgcccgcgtg cgcggcgtaa tggcggctgc gatcttggtc tcgtcgatgt tgagcgtatc
     1921 gggacggatg tcgacgaaga ccgggaccgc accgcgcagc acgaaggcat tggccgtcga
     1981 aacgaaagtg aaagacggca tgattacctc atcgccggga ccgatgccgg cgaggatcgc
     2041 cgccatctcg agcgcggcgg tgcacgaatg ggtgaggagc gccttgcggc aaccgg
//
LOCUS       DASUNT010000666         3419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_260410, whole genome shotgun sequence.
ACCESSION   DASUNT010000666 DASUNT010000000
VERSION     DASUNT010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260410"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VFX94_13345"
     CDS             complement(<1..500)
                     /locus_tag="VFX94_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006752620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5769213.1"
                     /translation="MDPSAVGLQSVLVLLASSVIAVAVCQSLRLPPMVGYLVTGLALG
                     PHVLGVVSEREEIHQLAEFGVVFLMFSIGLEFSLSKLKSMRRLVFGLGLAQVAATIAL
                     GILAAAALGASWQVGLALGGVAAMSSTAIVSKLLAERGELDSTHARQVIAVLLFQDLA
                     VVPLLVL"
     gene            555..1535
                     /locus_tag="VFX94_13350"
     CDS             555..1535
                     /locus_tag="VFX94_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX5769214.1"
                     /translation="MDPTLELARRVLEIEAEAVRALIERVDQRFAAAVELIDKRKGRV
                     VVSGMGKSGHIARKVASTLASTGTPAYFVHPAEASHGDLGMIENGDVFIAISYSGASE
                     ELVQIVPQIKRRGAKLIAITGKADSALAREADVHLDAAVAQEACPHNLAPTASTTAAL
                     ALGDALAVALLELRGFSAADFARTHPRGGLPAQALARQALLHVEDVMRKGERVPKVAV
                     GTPLRQAVAEMSRGGIGMTAVLDAAGVVQGVFTDGDLRRTIEKGAELGALKVDQVMTR
                     NPRTLRSHTLAAEAVHVMEEHKVNQVLVIDERGALVGALNMHDLFRARVI"
     gene            1583..2056
                     /locus_tag="VFX94_13355"
     CDS             1583..2056
                     /locus_tag="VFX94_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX5769215.1"
                     /translation="MIFDVDGVLTDGTLLYGPRGEALKAFSAHDGHGLKMLAESGVAC
                     ALLSGRRSAAVARRAAELGIKHVLQGIEDKLAAARALAIPLDRVGFMGDELVDLPVLT
                     RCGFACAPREAPEAVKSRAHYVARAAAGRGAAREVCELVMRAQSTLEPALARYLG"
     gene            2056..2628
                     /gene="lptC"
                     /locus_tag="VFX94_13360"
     CDS             2056..2628
                     /gene="lptC"
                     /locus_tag="VFX94_13360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX5769216.1"
                     /translation="MFSTTRLFPLGVMLALALLTFYLERTVRDEDLPAAPRRHDPDYF
                     VVNFTTTTYTGEGAVESVMSAARMVHYPDDDTTELFDPRMLHSKPNEPRFSVRAERGQ
                     LSRDGDEIFLYGDVLLVREAAAGRPEARMSTEFLHVLRDRSLVRTDRPVTIVEGGLSL
                     AGRGMEYNNASRELLLRNEVVARFDRAGSE"
     gene            2625..3149
                     /gene="lptA"
                     /locus_tag="VFX94_13365"
     CDS             2625..3149
                     /gene="lptA"
                     /locus_tag="VFX94_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEX5769217.1"
                     /translation="MKRALLAATLCLVSVCAFAEKADRDKPTQVEANRMSADDARRLN
                     VFEGDVVVSKGTMRLNADRLVVRQDAEGFQSATATGRPARFRQRQDPKPGEKEGIWVE
                     GEALRIELDDREQKIELFERARVNRGGDEVAGDYILVDQRSEFYQVSSGKGAAKGRVK
                     AVIQPKPSGAEAGK"
     gene            3158..>3419
                     /locus_tag="VFX94_13370"
     CDS             3158..>3419
                     /locus_tag="VFX94_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5769218.1"
                     /translation="MGSLLRSQRLRKRYKSRTVVQDVSLEVSSGEVVGLLGPNGAGKT
                     TCFYMIVGLIPVDSGSIHLDSRELTHLPIHRRARLGISYLPQE"
BASE COUNT          550 a         1185 c         1213 g          471 t
ORIGIN      
        1 agcacgagca gcggcacgac cgcgaggtcc tggaagagca gcaccgcgat cacctgccgg
       61 gcgtgcgtgc tgtcgagctc gccgcgctcg gcgagcagct tgctcacgat cgcggtcgac
      121 gacatcgcgg cgacgccgcc gagcgcgagg cccacctgcc acgaagcccc gagcgccgcc
      181 gccgccagga ttccgagcgc gatggtggca gccacctgcg cgaggcccag gccgaacacc
      241 aggcggcgca tcgacttcag cttcgacagg ctgaactcca gcccgatcga gaacatgagg
      301 aaaacgacgc cgaactcggc gagctggtgg atctcctcgc gctcggacac cacgcccagc
      361 acatgcgggc cgagggcgag accggtcacg agatagccga ccatcggcgg cagccgaagg
      421 ctctggcaca cggcgaccgc gatcacgctc gacgcgagca gaacgagcac cgactgcagg
      481 ccgactgcgc tggggtccat gggctatact ccgcggcgat gataaaggca gcccgcagcg
      541 tggggaaaag cagcatggat cccaccctcg agctcgcgcg gcgggtgctc gagatcgagg
      601 ccgaggccgt gcgcgcgctg atcgagcgcg tcgaccagcg cttcgccgcc gcggtcgagc
      661 tgatcgacaa gcgcaagggc cgcgtcgtgg tgagcggcat gggcaagtcg gggcacatcg
      721 cgcgcaaggt ggcctccacg ctggcgagca ccggcacgcc agcctatttc gtccatcccg
      781 ccgaggcgag ccatggcgac ctcggcatga tcgagaacgg cgacgtcttc atcgcgatct
      841 cctattcggg cgcgagcgag gagctggtgc agatcgtgcc gcagatcaag cgccgcggcg
      901 ccaagctgat cgccatcacg ggcaaggccg actccgcgct cgcgcgcgaa gccgacgtgc
      961 atctcgacgc ggcggtcgcc caggaggcct gcccgcacaa cctcgcgccg accgcgagca
     1021 ctaccgcggc gcttgcgctc ggcgatgcgc ttgccgtcgc gctgctcgag ctgcgcgggt
     1081 tctcggccgc cgacttcgcg cgcacccacc cgcgcggcgg gctcccggcc caggcgctgg
     1141 cgcgccaggc gcttctgcac gtggaagacg tcatgcgcaa gggcgagcga gtgccgaagg
     1201 ttgccgtcgg cacgccgctc aggcaggcgg tcgccgagat gtcgcgcggc gggatcggga
     1261 tgacggccgt tctcgatgct gcaggcgtgg tgcaaggcgt attcaccgac ggtgaccttc
     1321 gccgcaccat cgagaagggc gccgagctcg gcgccctgaa ggtcgaccag gtcatgacgc
     1381 gcaacccgcg cacgctgcgc tcgcatacgc tcgcggcgga agcggtgcat gtgatggaag
     1441 agcataaggt caatcaggtc ctcgtcatcg acgagcgggg cgcgctggtg ggcgcgctca
     1501 acatgcacga tctcttccgc gccagggtga tttgagcgag gtttcccagc aaacgctcga
     1561 cagagcgcgc ggcgtgcgac tgatgatttt cgacgtcgac ggcgtcctga ccgacggcac
     1621 tctgctctat gggccgcgcg gcgaggccct gaaggcgttc tcggcgcacg acggtcacgg
     1681 cctgaagatg ctcgccgaga gcggcgtggc gtgcgcgctg ctctccggcc gacgctcggc
     1741 agcggtggcg cggcgtgccg ccgagcttgg catcaagcac gtgctccagg gcatagagga
     1801 caagctcgcc gcggcgcgcg cgctcgcaat tcccctggac cgggtcggct tcatgggcga
     1861 tgagctggtg gacctcccgg tgctgacgcg ctgcggcttc gcgtgcgcgc cacgcgaagc
     1921 gcccgaggcg gtgaagagcc gggcgcacta tgtggcgcgc gcggccgcgg gccgcggcgc
     1981 cgcgcgcgag gtgtgcgagc tcgtcatgcg cgcgcaaagc acgctcgaac cggcgctcgc
     2041 gcgctacctc ggctgatgtt ctccacgacg cggctcttcc cgctgggcgt gatgctcgcg
     2101 ctcgccctgc tcacgttcta cctcgagcgc acggtgcgcg acgaggacct gcccgccgcg
     2161 ccgcggcgcc acgaccccga ttacttcgtc gtcaacttca ccaccaccac ctacaccggc
     2221 gaaggcgccg tggaaagcgt catgtcggcg gcgcgcatgg tgcactaccc ggacgacgac
     2281 accaccgagc tcttcgaccc gcgcatgctg cattccaagc cgaacgagcc gcgcttcagc
     2341 gtgcgcgccg agcgcggcca gctttcgcgc gacggcgacg agatcttcct gtacggcgat
     2401 gtgctgctgg tgcgcgaagc ggccgccggg cggcccgagg cgcgcatgag caccgagttc
     2461 ctgcacgtgc tgcgcgaccg ctcgctcgtg cgcaccgacc gcccggtcac gattgtggag
     2521 ggcggcctgt cgctcgcggg gcggggcatg gagtacaaca acgcttcgcg cgagctcctg
     2581 ctgcgcaacg aagtcgtggc gcgcttcgac cgggcgggct ccgaatgaag cgcgcgctgc
     2641 ttgcggcgac gctttgcctc gtctcggtct gcgcgttcgc ggagaaggcc gaccgcgaca
     2701 agccgactca ggtcgaggcg aaccgcatga gtgccgacga cgcgcgccgc ctgaacgtgt
     2761 tcgaaggcga tgtcgtcgtc agcaagggga cgatgcgtct caacgccgac cgcctcgtgg
     2821 tgcggcagga cgccgagggc ttccagtcgg ccacggcgac cggccgtccg gcgcgctttc
     2881 gccagcgcca ggacccgaag ccgggcgaga aggaaggcat ctgggtcgag ggcgaggcgc
     2941 tgcgcatcga gctcgacgac cgcgaacaga agatcgagct cttcgagcgc gcgcgcgtca
     3001 accgcggcgg cgacgaggtg gcgggcgact acatcttggt cgaccagcgc tcggagttct
     3061 accaggtgag ctcgggcaag ggtgcggcca agggccgcgt caaggccgtc atccagccca
     3121 agccttccgg cgccgaagcc ggcaagtgac cgcgccgatg ggtagcctgc tacgctcgca
     3181 gcggctgcgc aagcgctaca agtcgcgcac cgtggtgcag gacgtgtccc tcgaggtgtc
     3241 gagcggcgag gtggtcgggc tgcttgggcc gaacggcgcc ggcaagacga cctgcttcta
     3301 catgatcgtg gggctgattc cggtcgactc gggctcgatc catctcgaca gccgcgagct
     3361 cacccacctg ccgatccacc gccgggcgcg gctggggatc tcctacctgc cgcaggaga
//
LOCUS       DASUNT010000667         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_84356, whole genome shotgun sequence.
ACCESSION   DASUNT010000667 DASUNT010000000
VERSION     DASUNT010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84356"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..972)
                     /locus_tag="VFX94_13375"
     CDS             complement(43..972)
                     /locus_tag="VFX94_13375"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5769219.1"
                     /translation="MKDRTVIALFALCAGLVASAWLAPQWLVFLLALAIAKGLVVLGL
                     LLLMRTGLVSFGQGLYFGLGAYASALLANRAGIADAAVMLVAGTACAALVAAVLGLLL
                     ARYREIFFAMLSLAFSMILYGLLVKSSTLGSTDGFNLHAKSFAGIALGAADARRVFYG
                     VSALAVLAVAWALHRYLASHWGRLSLAIRDNELRVEYLGASVYRVVYVNYVVAAALAG
                     LGGAFAALAVGHVDPETVYWTTSGEFVFIAILAGTGNVLAPFFSAAIFEALRTLASQY
                     APNIWQMALGAAMLAIIMFLPGGLWTLVRRKPA"
     gene            complement(969..1856)
                     /locus_tag="VFX94_13380"
     CDS             complement(969..1856)
                     /locus_tag="VFX94_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5769220.1"
                     /translation="MLTVVFDGVVYSSWLFLMAAGLTLIYGVMRILNIAHGSLYAIGA
                     YAAASLGAAWVASGHAAWGSYAALALSALAVGLVMGPLIERGLLKWMYGKDEIVLLLI
                     TYALFLILEDAIKLVWGVESKMFSEPYALLGNFQLGGLAYPAYNLVLIAVAVLIGLAL
                     SWVLHRTRQGKLLLAVIHDREVSAAMGINVSRFYLATFTGGAVLAALGGALTAPIIAV
                     SPGIGVEVIVLSFAVVVIGGLGSLGGAALGALMVGLLRSVAVRYWPEGELFVIYLVMA
                     GVLILRPKGLFSQPEARRI"
     gene            complement(1872..>2566)
                     /locus_tag="VFX94_13385"
     CDS             complement(1872..>2566)
                     /locus_tag="VFX94_13385"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5769221.1"
                     /translation="DFEAAMKALKPGIEVKTSLMTKLFAGQFGAEISTLLSSGAELVH
                     SSFWGADLEGLVLQGAPRGLFQKHIVLLSAGEPAINRLGTRIPDGTIIGARGPFGPFA
                     PDTEFNRWFRTTFQDRYGVPPNYAAYKATNALLGLKAAYEKAQKAGAQTPSQEQIIAA
                     FENLAFDGVGGTVRMSLGKGHQAVMDNAIGTARTVGGQLTIVDVKRYAAEQVNPPEGM
                     KSEAWIKSGLKK"
BASE COUNT          494 a          860 c          853 g          359 t
ORIGIN      
        1 cgccgaaacg cttgtccagc cgctcggcgg aaagcagcgc gctcacgcgg gctttctcct
       61 gaccagcgtc catagtccgc cgggcaggaa catgatgatc gcgagcatcg ctgcgccgag
      121 ggccatctgc cagatattgg gcgcgtactg gcttgccagg gtgcgcagcg cctcgaagat
      181 cgccgcggag aaaaacggcg cgagcacatt gcccgtgccg gccaggatgg cgatgaacac
      241 gaactcgccc gaggtcgtcc agtacaccgt ctccgggtcg acgtggccga ccgccagcgc
      301 ggcgaacgcg ccgcccaggc cggcgagcgc tgccgcgacc acgtagttca cgtacaccac
      361 gcggtagacg gacgcgccca ggtactcgac ccgcaactcg ttgtcgcgga tcgccaagga
      421 gagccgcccc caatgcgagg caagatagcg gtgcagcgcc caggcgacgg cgagaaccgc
      481 gagcgcggag acgccgtaga acacgcggcg cgcgtcggcg gcgccgagcg cgatcccggc
      541 gaacgacttt gcgtgcaagt tgaacccgtc ggtgctgccc agggtcgagc ttttcacgag
      601 cagcccgtag aggatcatcg agaaagcgag cgacagcatc gcgaagaaga tctcgcggta
      661 gcgcgcaagc agcagcccaa gcaccgcggc gacaagggcg gcgcaagcag tgccggcaac
      721 gagcatcacg gccgcgtcgg cgatcccggc gcggttggca agcagcgccg acgcgtaggc
      781 gccgaggccg aagtagagtc cctggccgaa cgaaacgagc ccggtgcgca tgaggagcag
      841 caggccgagc accaccaatc ccttcgcgat cgccagcgcg agcaggaaca ccagccattg
      901 cggcgcgagc cacgccgagg cgacgagacc cgcgcacagc gcaaagagcg cgatgactgt
      961 tcgatctttc atatgcggcg tgcctcgggc tggctgaaca ggcccttcgg cctgaggatc
     1021 agcacaccgg ccatgaccag gtagatgacg aagagctcgc cctccggcca gtagcgtact
     1081 gccaccgagc gcagcaaccc gaccatcagc gcgccgagcg ccgcgccgcc gaggctgccg
     1141 agcccgccga tcacgaccac ggcgaacgaa agcacgatga cttcgacccc gatccccggg
     1201 ctgaccgcga tgatgggggc ggtcagcgct ccgccgagcg ccgccagcac ggcgccgccc
     1261 gtgaaggtgg cgaggtaaaa gcggctgacg ttgataccca tggccgcgct cacctcacgg
     1321 tcgtgaatca cggcaagcaa gagctttccc tggcgcgtgc gatggagcac ccaggacaaa
     1381 gcgagcccga tcaggaccgc gaccgcgatc agaaccaggt tatacgccgg atacgccagg
     1441 ccgccgagct ggaagttgcc gagcagcgca tagggctccg agaacatctt ggactcgacg
     1501 ccccagacga gcttgatggc gtcttccagt atgagaaaga gggcgtaggt aatgagcaga
     1561 agcacgatct cgtccttccc gtacatccat ttgagaaggc cgcgctcgat cagcggtccc
     1621 attaccagac cgaccgcaag cgcggacagg gcgagcgcgg cgtagcttcc ccacgccgcg
     1681 tgtccgcttg cgacccaggc cgcgccgagc gaagcggccg cgtaagcgcc gatcgcgtag
     1741 aggctgccgt gtgcgatgtt gagaatgcgc atcacgccgt agatcagcgt gagcccggcg
     1801 gccatgagga acagccacga ggaatagacc acgccgtcga agacgacggt gagcatccgc
     1861 tcagcgccga gctacttctt cagccccgac ttgatccagg cttcgctctt cattccctcg
     1921 ggcggattca cctgctcggc ggcatagcgc ttgacgtcga caatcgtgag ctgcccgcct
     1981 acggtcctcg ccgtgccgat ggcgttgtcc atgacggcct ggtggccctt gccgagcgac
     2041 atgcgcaccg tgccaccgac gccgtcgaag gccagatttt cgaacgcggc gatgatttgt
     2101 tcctggctcg gggtttgagc gccggccttc tgagccttct cgtacgcggc cttgaggcca
     2161 agcagcgcgt tcgtcgcctt gtacgcggca tagttgggcg gcacgccata gcggtcctgg
     2221 aacgtggtcc tgaaccagcg gttgaactcg gtgtccggcg cgaacgggcc gaacggaccg
     2281 cgcgcgccga tgatcgttcc gtccgggatc cgtgtgccga ggcggttgat ggccggctcg
     2341 ccggccgaga gcagcacgat gtgcttctgg aaaagcccgc gcggcgcgcc ctgcagcacg
     2401 aggccttcga ggtcggcgcc ccagaagctc gaatgcacga gttcggcgcc gctcgagagc
     2461 agcgtcgaga tctccgcgcc gaactgcccg gcgaagagct tcgtcatgag cgaggtcttc
     2521 acctcgatcc cgggcttgag ggccttcatc gcggcctcga agtcgg
//
LOCUS       DASUNT010000668         3381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_610144, whole genome shotgun sequence.
ACCESSION   DASUNT010000668 DASUNT010000000
VERSION     DASUNT010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610144"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VFX94_13390"
     CDS             complement(<1..388)
                     /locus_tag="VFX94_13390"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEX5769222.1"
                     /translation="MDRLERFYKIDQLLKERKVVSFAAFKEVLGMSRASVKRDLEYMR
                     SRFNAPIEYDRRANGYRFGRPRSGPRYELPGLWFNAAEVQALLTTLQLLANLQPGLLD
                     GQVRPVLERLRSILGKGDHSWEEVVKR"
     gene            complement(435..611)
                     /locus_tag="VFX94_13395"
     CDS             complement(435..611)
                     /locus_tag="VFX94_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769223.1"
                     /translation="MSKRPELYLIHGRADGDERKVFRLRETRPHEAPAENWPADGEWR
                     ELVRRLTDNPVKSE"
     gene            complement(745..978)
                     /locus_tag="VFX94_13400"
     CDS             complement(745..978)
                     /locus_tag="VFX94_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein CsbD"
                     /protein_id="HEX5769224.1"
                     /translation="MDWITIETRWDEYKVAAKQQWDKLSEQQLHGTRGKREYLVRRVQ
                     EAYSLSRGDAERQISEWLAKQAEKRAGTESSTR"
     gene            1167..>3381
                     /locus_tag="VFX94_13405"
     CDS             1167..>3381
                     /locus_tag="VFX94_13405"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5769225.1"
                     /translation="MRTEIGERVQRIEDPLLLRGEGRYTDDLNEPGQAYAYIVRSPHA
                     HGVIRNISLENARAMPGVVAVYTAADLAAYGPHKCALDFKQRDGTPMKKPIRKSLAQD
                     KVRFVGDPVACVVAETYVQAKDAAEAVELDIEPLPAVTLPSEAAKPGAPQLYDDVPNN
                     VCLDFLWGEPEKVAAAFAGAAHVTKLSLRNTRVIVAAMEPRAGLCSFDKANQKWTLTV
                     PGQGVWGQKTQLVDMLGVTPDKVRIRSYHVGGSFGMKGSIYPEYICLVHAARALGRPV
                     KWTDERSGSFLSDQHGRDHEMSAELALDKEGKFLALRITGYGNVGAYLGTVAPQPPSM
                     NVVRNVCGVYRIPLLEVSTKVCFTNTTPVSAYRGAGRPEANMYIERLIDETARQLRIE
                     RFELRRRNHLKPSEIPYKNAANMTVDSGDFGTIFEKALKEADVANFASRKTESRKRGR
                     LRGLGVGSYMEVTAPPNKEMGGIRFEGDKRITFITGTLDYGQGHASPMAQVLSARLGV
                     PFETISLKQSDSDALVFGAGTGGSRSAMMSGSAAIEAAALVIKKGRALAAEELEAAEA
                     DIEFREGRFTVVGTDKSISLLELAGKRPQALDVTHVTEVIPSAFPNGCHVCEVEIDPD
                     TGAVEVVRYSSVNDFGTIVTPLMVEGQIHGGVVQGMGQCFMESARYDETGQLVTGSFM
                     DYALPRANDFTAPFPWQSHPVPATTNPLGAKGCGEAGCAGSMTSVMNAVVDALSEL"
BASE COUNT          565 a         1093 c         1155 g          568 t
ORIGIN      
        1 tgcgcttgac gacctcttcc catgagtggt cgcccttgcc gaggatcgag cgcaggcgct
       61 cgagcaccgg cctcacctgg ccgtcgagca gtccgggctg gaggttggcg agaagctgca
      121 gcgtggtgag gagcgcctgg acctcggcgg cgttgaacca caggcccggc agctcgtagc
      181 gcggccccga gcgcggtctg ccgaagcggt agccgttcgc ccggcggtca tactcgatcg
      241 gggcgttgaa gcggctgcgc atgtactcga ggtcgcgctt cacgctcgcg cgcgacatcc
      301 cgagcacttc cttgaaggcg gcaaacgaaa cgaccttgcg ctccttgagg agctggtcga
      361 tcttgtagaa ccgctcgagc ctgtccatgc gccccgcacg cccgccggcg aggccgagat
      421 cttactcgct tgcgctactc gctcttgacg gggttgtccg tcagccggcg cacgagctcg
      481 cgccactcac cgtccgccgg ccagttctcg gcgggggctt cgtgcgggcg ggtttcgcgc
      541 aggcggaata ccttgcgctc gtccccgtct gcgcgcccgt ggatcaggta aagctcgggg
      601 cgcttgctca ttgcgcttcg ccgtcgggcc gcggcgccgg cgcttcggcg gggcggcgca
      661 tcgctgccgc ggctgccgcc accagggtga cgctgccgca catccattgc tcgttcgtca
      721 tgggctcggt tgcttcttcc cggatcatcg ggtcgagctc tctgtgcccg cgcgcttctc
      781 cgcctgcttc gccagccatt cggaaatctg gcgctcggcg tctcccctgc tcaatgaata
      841 ggcttcctgc acgcgcctca ccagatactc gcgcttgccc cgcgtgccat ggagctgctg
      901 ctcggagagc ttgtcccact gctgcttggc ggcgaccttg tattcgtccc agcgggtttc
      961 gatggtgatc cagtccactt gtgcctccgt tcgcgttgtt cgagctgcgg gcgatgcggg
     1021 ttgctgctgc gtagcgggag gggctgaggt atcgccggca aggcggcggt gcgacaggct
     1081 gcttccggaa ccaggacacc atactggccg ctctgcgtgc ggtcaaggca tttcggtaag
     1141 cggcgcgcgt ttgcaataat ttggccatgc gaactgaaat cggcgagcgc gtacagcgca
     1201 tcgaggaccc tctattgctc cgcggcgaag gccgctacac cgacgacctg aacgagcccg
     1261 ggcaggcata tgcgtacatc gtgagaagtc cgcatgccca cggagtcata agaaacatct
     1321 cattggaaaa cgcgagggcg atgcccggcg tcgtcgcggt ctacaccgcc gccgacctcg
     1381 cggcctacgg cccgcacaag tgcgcgctcg atttcaagca gcgcgacggc acgccgatga
     1441 agaagccgat ccgcaagtcg ctcgcgcagg acaaggtgcg cttcgtgggc gacccggtgg
     1501 cgtgcgtggt ggccgagacc tacgtccagg cgaaggacgc ggccgaggcg gtggagctcg
     1561 acatcgagcc gctgccggcg gtgacgctgc cctccgaggc ggcgaagccg ggcgcgccgc
     1621 agctctacga cgacgtgccg aacaatgtct gcctcgactt cctttggggc gagccggaaa
     1681 aagtggcggc cgccttcgcg ggggcggccc acgtaacgaa attgtctttg cgcaatacga
     1741 gagtcatcgt cgcggcgatg gagcccaggg ccgggctctg ttcattcgat aaggcaaatc
     1801 agaagtggac gctgaccgtc cccggccagg gggtgtgggg gcagaagacg cagctcgtcg
     1861 acatgctcgg cgtgaccccc gacaaggtgc gcatccgctc ctaccacgtc ggcggctcgt
     1921 tcgggatgaa aggctcgatc tacccggagt acatctgcct cgtgcacgca gcgcgggcgc
     1981 tcgggcggcc ggtgaaatgg accgacgagc gctcgggttc gttcctttcc gaccagcacg
     2041 gccgcgacca cgagatgagc gccgagctcg cgctcgacaa ggaagggaag tttctcgctt
     2101 tgaggatcac cggctacggc aacgtcggcg catacctcgg cacggtggcg ccgcagccgc
     2161 cgtcgatgaa cgtggtgcgt aacgtgtgcg gcgtgtaccg catcccgctg ctggaagtgt
     2221 cgaccaaggt gtgcttcacc aacaccaccc ccgtctcggc gtaccgcggc gcggggcgcc
     2281 cggaggcgaa catgtacatc gagcgcctga tcgacgagac ggcgcgacag ctgcgcatcg
     2341 agcgcttcga gctcaggcgc cgcaaccacc tgaagccctc ggagatcccg tacaagaacg
     2401 ccgcgaacat gaccgtcgac agcggcgatt tcgggacgat tttcgagaag gcgctcaagg
     2461 aagcagatgt ggcgaatttc gcttcgagaa aaactgaaag caggaagcgc ggcaggctgc
     2521 gcggcctcgg cgtggggagc tacatggaag tgaccgcgcc gccgaacaag gagatgggcg
     2581 ggatccgatt cgaaggcgac aagcgcatca ccttcatcac cggcacgctc gactacggcc
     2641 aggggcacgc ctcgccgatg gcgcaggtgc tctccgcgcg cctcggtgtc ccgtttgaaa
     2701 ccatttcctt gaaacaaagc gattcagacg cactcgtgtt cggcgccggc accggcggct
     2761 cgcgcagcgc gatgatgagc ggctcggcgg cgatcgaggc ggcggcgctc gtgatcaaga
     2821 agggaagagc gctcgcggcg gaagagctcg aggcggccga ggcggatatc gagttccgcg
     2881 aaggacgctt tacggtcgtc ggtacggaca agtcgatttc gctgctcgag ctggcgggga
     2941 aaagacccca ggcgctggat gtcacccatg tcaccgaggt gattccctcg gcctttccca
     3001 acggctgcca cgtgtgcgag gtcgagatcg acccggacac gggcgcggtc gaggtggtgc
     3061 gctacagctc ggtgaacgac ttcggcacga tcgtgacccc gctcatggtc gagggccaga
     3121 tccacggcgg cgtggtgcag ggcatgggcc agtgcttcat ggagagcgcg cgctacgacg
     3181 agaccggcca gctcgtcacg ggctcgttca tggactacgc gctgccgcgc gcgaacgact
     3241 tcaccgcgcc cttcccctgg cagagccacc cggtgcccgc gaccaccaat ccgctcggcg
     3301 cgaagggctg cggcgaggcg ggctgcgcgg ggtcgatgac ttccgtcatg aacgcggtgg
     3361 tggatgcgct ctcggagctc g
//
LOCUS       DASUNT010000669         4300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_524159, whole genome shotgun sequence.
ACCESSION   DASUNT010000669 DASUNT010000000
VERSION     DASUNT010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524159"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..992
                     /locus_tag="VFX94_13410"
     CDS             87..992
                     /locus_tag="VFX94_13410"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5769226.1"
                     /translation="MNLPDVRQLRYFIAVAETLHFGRAAAALHISQPPLSRSIAELED
                     RVGTRLLARTRRRVELTAEGARFLVEARRVLSQLEHAVLDVRGIAAGTTGRLRIGFVS
                     LADYGVLPRLLKAYKASRPGVELALREMLSPEQAAALAAGELDFGLLLPPVAGVSLEH
                     FVVQRERFVIALPARHRLAQRPGRVTLRELSEEAFVMVPRDIAPGLYDIVAALAARAG
                     FAPRVAQEAIQMQTVVSLVSSGLGVALVPGSIANLGRRGVIYRELADPHPRLELWLAW
                     QRSGLGAAGREFVEQARKALRKAAA"
     gene            989..1759
                     /gene="lgt"
                     /locus_tag="VFX94_13415"
     CDS             989..1759
                     /gene="lgt"
                     /locus_tag="VFX94_13415"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX5769227.1"
                     /translation="MIAHPNIDPIAFSIGPLAVRWYGLMYLAGFAIGWWLGARRIARG
                     QAPITRAQLDDLLFLVVLGVIIGGRLGYVLFYKPGYYASHPFEIIAIWQGGMSFHGGL
                     LGVMAAMGVAAHRHGVDYVRLMDFIAPLIPPGIAAGRLGNFINAELPGRVTDVPWAMV
                     FPGTDGAPRHPSQLYQFALEGVALFVLLWWFSSKPRPRGQISAMFLIGYGVLRFITEF
                     AREPDSFIGYLALGLTMGQWLCLAMIAGGAALLAWSRR"
     gene            1744..2676
                     /locus_tag="VFX94_13420"
     CDS             1744..2676
                     /locus_tag="VFX94_13420"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX5769228.1"
                     /translation="MESALDPVLGAVLPVFGLVGCGYLAGRLRIVTQASSAALNQFVY
                     AFALPAMLFIAVYRGSLDEILSGAFMLAVIAATLGTAVAGFVISNLSGARPAESTMRA
                     LNASFANTGYLGIPLVTVAYGEAAALPAALATVATNFVSFAVAIVCLELFVSPRPGAL
                     RRALRGVIMSPLIWPIVLAIVLVAVEVKIPLPLERLAALLAAAAGPCALFAIGLFVSQ
                     LSIRAGAVASWQTTVLKLVLHPVLMALLVFLVFRIDPLWAKIAVVGAALPLGATAFVL
                     AQRYQLLEAETSSGAVVSTAASLLTVSLVMALLG"
     gene            complement(2673..>4300)
                     /locus_tag="VFX94_13425"
     CDS             complement(2673..>4300)
                     /locus_tag="VFX94_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5769229.1"
                     /translation="SFIEDCAVPLEHLAEYTAQLTEVFRKHGTEGTWYAHASVGCLHV
                     RPVLNMKGDGAMRMRAIAEEAADLVRRYKGAYSGEHGDGLVRSEWIAPFFGPRLTAAL
                     GEIKSWLDPKGLMNPGKIVNPSRMDERTLFRFKPGYAAVVAPSVLDWSEWGGYDKAVE
                     MCNNNGHCRKFDAGTMCPSYRATMDEQHLTRGRANTLRLALSGQLGDEGEKAVKDALE
                     LCVSCKGCKRECPTGVDMARMKIEFLAHYKAKHGWSARDKAIGYLPRYAAWAARLAPL
                     SNLASSLTKRALGFSDKRSLPKWRTDYFAQEGNRDADLVLFVDTFNRYFEPQNARAAF
                     KVLQAAGYRVHAPRGLCCGRTYLSVGLVDEAKREARKVIDALKPYVKRGVPIVGLEPS
                     CLFSLRDEFPVLAGDHLAKASYLFEEFLATNPGKLKFKEMKREALLHGHCHQKAFDAM
                     AAVEKVLGMVPGLKVSTVQTSCCGMAGSFGYEAEHYGVSMKMAELSLLPAIRSSSHET
                     LLVADGTSCRHQIADASGRAAVHVAQVLERALA"
BASE COUNT          589 a         1477 c         1426 g          808 t
ORIGIN      
        1 gggtttgctc cttgtcgcgt gcattgtgcg cgccgcgcgc cccgcaatcc aatgcaattt
       61 cgggggcgct gtaatacctt cgcggtatga atcttccgga cgtgcgccag ttgcgctatt
      121 tcatcgcggt cgccgagacg cttcattttg gccgcgctgc ggcggcgctg catatttcgc
      181 agccgcccct ttcgcgttcg atcgccgagc tggaggatcg cgtcggcacc cggctgctcg
      241 cgcgcacgcg ccggcgtgtc gagctcaccg ccgagggcgc gcgcttcctc gttgaagcga
      301 ggcgcgtcct gtcgcagctc gagcacgcgg tgctcgacgt ccgcggcatc gcggcgggca
      361 cgaccggccg gctgcgcatc gggttcgttt cgctcgcgga ctatggcgtg ctgccgcggc
      421 tcctgaaggc gtacaaggcc tcgcgcccgg gcgttgagct cgcgctgcgc gagatgctct
      481 cgcctgagca ggccgcggct cttgctgcgg gcgagctcga cttcgggctg ctccttccgc
      541 ccgtggcggg cgtatcgctg gaacacttcg tggtgcagcg cgagcgcttc gtcatcgcct
      601 taccggcccg gcaccggctt gcgcagcgcc cgggccgggt gacgctgcgc gagctcagcg
      661 aagaagcctt cgtcatggtg ccacgcgata tcgccccagg gctctacgat atagtcgccg
      721 ctctagccgc gcgcgccggg ttcgcgcccc gagtggcgca ggaggcgata cagatgcaga
      781 cggtggtgag tctggtgtcg agcgggctcg gcgtggcgct cgttcctggt tcgatcgcga
      841 acctcggccg caggggcgtg atctatcgcg agctcgccga cccgcacccg cggctggagc
      901 tgtggctcgc ctggcagcgc agcggccttg gcgcggccgg gcgcgagttc gtcgagcagg
      961 cgagaaaggc cctgcggaaa gcggccgcgt gatcgcgcac ccgaacatcg atccgatcgc
     1021 gttttcgatc gggccactcg cggtgcgctg gtacggcctc atgtacctcg ccgggttcgc
     1081 catcggctgg tggctcggcg cgcgcagaat cgcaagaggg caggcgccga ttacgcgcgc
     1141 acagctcgat gacctgctgt ttttggtcgt gctcggagtc atcatcggcg gccgcctggg
     1201 ttacgtgctt ttctacaagc ccggctacta cgcgtcccat ccattcgaga tcatcgccat
     1261 ctggcagggc ggcatgtcgt tccacggcgg gctgctcggc gtgatggcgg cgatgggcgt
     1321 cgccgcgcat cgccacggcg tcgactacgt gcgcctcatg gacttcatcg cgccgctcat
     1381 cccgccgggg atcgcggccg gcaggctggg caacttcatc aacgccgagc tgcccggccg
     1441 ggtgacggat gttccatggg caatggtgtt cccgggcacc gacggcgcgc cgcgccatcc
     1501 ctcacagctt taccagttcg cgctcgaggg cgtggcgctg ttcgtgcttc tgtggtggtt
     1561 ttcctcgaag ccgcggccgc gcggccaaat ctcggcgatg ttcttgatcg gctacggggt
     1621 gctgcgcttc attaccgagt tcgcccgcga gcccgattct ttcatcggat atctcgcgtt
     1681 ggggctcacc atggggcaat ggctatgcct cgcgatgatc gccggcggcg ccgcgctcct
     1741 cgcatggagt cggcgctaga cccggttctc ggcgcggtgc tgccggtctt cggactggtc
     1801 ggctgcggat acctggcagg gcgcttgcgt atcgtcacgc aggcatccag cgcagccctg
     1861 aaccagttcg tctatgcctt cgccctgccc gcgatgctct tcatcgcggt gtaccgcggc
     1921 tccctggacg aaatcctgag cggcgctttc atgctcgccg tcatcgccgc gacgctcggc
     1981 actgcagtgg cgggattcgt catttcgaac ctgtccggtg cccggcccgc ggaaagcacg
     2041 atgcgggcgt tgaacgcatc cttcgccaat acgggctacc tcggcattcc gctcgtcacc
     2101 gtggcctacg gcgaggccgc agcgctgccg gcagcgcttg ccaccgtagc gacgaatttc
     2161 gtttcgttcg ccgtggccat cgtatgcctg gagctcttcg tcagcccgcg ccccggggcg
     2221 ctacgccggg cgctgcgtgg cgtgatcatg agcccgctca tctggccaat cgtgctcgcc
     2281 atcgtcctgg tggccgtgga ggtgaagatt ccgctgccgc tcgagcgcct cgcggcgctg
     2341 ctcgcggccg cagcggggcc gtgcgcgctc ttcgccatcg ggctcttcgt gtcgcagctc
     2401 tccatccgcg ccggcgcggt cgcgagctgg cagacgaccg tgctcaagct cgtgctgcac
     2461 cccgtgctga tggcgctgct cgtcttcctg gtttttcgaa tcgacccgtt gtgggcgaag
     2521 atcgccgtcg tgggcgccgc cctgccgctc ggcgccaccg ccttcgtcct cgcgcagcgc
     2581 taccaactgc tcgaagccga aacctcgagc ggcgccgtcg tgtcgaccgc ggcttcgctt
     2641 cttaccgttt cgctggtaat ggcgctgctc ggctaggcga gcgcgcgctc cagcacctga
     2701 gcaacgtgca cggcggcgcg accgctcgcg tcggcgatct ggtggcggca gctcgtaccg
     2761 tcggccacga ggagcgtctc gtgcgacgag ctgcggatcg cggggagcag cgaaagctcg
     2821 gccatcttca tcgagacgcc gtagtgctcg gcctcgtagc cgaagctccc cgccatgccg
     2881 cagcagctcg tctgcaccgt ggacaccttc aaccccggaa ccatgccgag caccttctcc
     2941 accgccgcca tggcgtcgaa cgccttctgg tggcaatggc cgtgcagcag cgcttcgcgc
     3001 ttcatctcct tgaacttcag cttgccgggg ttcgtggcca gaaattcctc gaagaggtat
     3061 gaggctttcg cgagatggtc gcccgccagg acaggaaact cgtcgcggag ggaaaacagg
     3121 cacgagggct cgaggcccac gatcggcacg ccgcgcttca cgtaaggctt cagcgcgtcg
     3181 atcaccttgc gcgcttcgcg cttggcttcg tctaccaacc ccacggagag gtaggtacgc
     3241 ccgcaacaca ggccgcgcgg cgcatgcacg cgatagccgg ccgcctgcag caccttgaac
     3301 gctgcgcgcg cgttctgcgg ctcgaagtag cggttgaacg tgtcgacgaa gagaacgaga
     3361 tcggcgtccc tgttcccttc ctgcgcaaag tagtcggtgc gccacttcgg cagactgcgc
     3421 ttgtcggaga agccaagcgc cctcttcgtc agggaagatg cgagattcga tagcggcgca
     3481 aggcgcgctg cccacgcggc atagcgaggc aggtagccga tcgccttgtc gcgcgcgctc
     3541 cagccgtgct tcgccttgta atgcgccagg aactcgatct tcatccgtgc catgtcgacg
     3601 ccggtgggac attcgcgctt gcagcccttg cacgatacgc aaagctccag cgcgtccttc
     3661 accgccttct cgccctcatc gccgagctgg ccggaaagcg cgaggcgaag ggtattcgcg
     3721 cgccctcggg tcaggtgctg ctcgtccatc gtcgcgcggt aggacgggca catcgtgccg
     3781 gcgtcgaact tgcggcagtg gccgttgttg ttgcacatct ccactgcctt gtcgtagccg
     3841 ccccactcgc tccagtcgag gacgctcggc gccaccaccg cggcgtagcc gggcttgaag
     3901 cgaaacagcg tgcgctcgtc catacgcgac gggttgacga tcttgccggg attcatcagc
     3961 cctttcgggt cgagccacga cttgatctcg ccgagcgcgg cggtaaggcg cgggccgaag
     4021 aacggcgcga tccattccga gcgcaccagg ccgtcgccgt gctcgccgga gtaggcgccc
     4081 ttgtagcggc gcaccaggtc cgcggcctcc tcggcgatgg cgcgcatgcg catggcgccg
     4141 tcgcctttca tgttgagcac cgggcgcacg tgcaggcatc cgaccgaggc atgcgcgtac
     4201 cacgttccct cggtgccgtg cttgcggaac acttccgtga gctgcgccgt gtactcggcg
     4261 aggtgctcca gcggcaccgc gcagtcctcg atgaaggaca
//
LOCUS       DASUNT010000670         4774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_698341, whole genome shotgun sequence.
ACCESSION   DASUNT010000670 DASUNT010000000
VERSION     DASUNT010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698341"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1412
                     /locus_tag="VFX94_13430"
     CDS             <1..1412
                     /locus_tag="VFX94_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5769230.1"
                     /translation="IGVLPGITPLVAIGMLFPLTFTLPPVSSLIMLAGIYYGAQYGGS
                     TTAVLVNLPGETAAAVTCIDGYQMARQGRAGPALAVAALASFFAGCIGTLLIALVGPP
                     LGEWALKFGPWEYCALMLMGLVASAVLAHGDAVKGLAMVALGLLFGIVGTDVNSGVPR
                     FAFDLPQLFDGIGFTVIAVGLFAVAEIAAKLESREARELFTGAIAQLMPSRRDVGDAA
                     WPTVRGTALGAAFGVLPGTGPALSSFAAYMLEKKLARDPGRFGHGAIEGVAAPEAANN
                     AAAQTGFIPTLTLGIPGSAVMTVMVGAMMMQGFAPGPGAMAEKPELFWGIIASMWIGN
                     LMLVVLNLPLVGLWVKLLSVPYRWLFPSIVMFCCIGTYSVTNNSFDVYLCAAFGLLGY
                     VLAKLECEPAPLLLGYVLGPLLEEHLRRAMILGRGDPLQFLARPISLAFVLGTALLLV
                     LMAWPALRRGKRLVGRLSQ"
     gene            complement(1402..1620)
                     /locus_tag="VFX94_13435"
     CDS             complement(1402..1620)
                     /locus_tag="VFX94_13435"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5769231.1"
                     /translation="MTGIRIGFSLSLIGTLLGEMFGSQHGVGHLLMQASLHSIERILA
                     LTLLLVVFAAGVNALLLALDRRLHGLIG"
     gene            complement(1649..3394)
                     /locus_tag="VFX94_13440"
     CDS             complement(1649..3394)
                     /locus_tag="VFX94_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004205743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX5769232.1"
                     /translation="MASIVIRSYASPALVLLAFDWPDADQHADFLGFSIRRTPGFYHQ
                     SRSWLPNRRRFAAPEEGKDYPSNEAPFQKFMWWDARIGPEHGDVRQIRYEAWPACGAP
                     GQVRLLEEAKAELRVDIPPHVVDGIGTWFNRAVVSSQAFSFMLREMGLTANQQPTPEQ
                     ALKLRSWLSAGMHTALVDHIEGAGSGKRQLAGAIYHLTDKLWTIPALAKSSEAGAAID
                     LVYDARTIKDAHGETMPPPTEKAVVELKDKVRFHPRNRTNIMHNKFIVSGKGFEGANS
                     KPKSVLMGSANFTTGGLTTQANLIHLFKSPGLAQSYLDRYDLLKANLTKGETAKKSGW
                     SPTETLGDAAIRVFFSPEPAKRRDSIETVVRAIHNARSSVMFCLFSPTDEALRHACFA
                     AGDRGLMMFGLVNNIADKEGEQVEDDMQADEKAAIELYHRSKDNRDVIEASRYRWDTK
                     PVGFVDEQQLFPGEPRPPFPPVIVHHKMVIIDAETPHPLIYTGSANMSKNSVNNNDEN
                     LLEILGSRRLAGMYLAEFLRLYEHYRARAFAPGSNKDDGRLKTTRDAWARRHLQPGTP
                     EFRARLAMTGRLLSG"
     gene            3644..4594
                     /locus_tag="VFX94_13445"
     CDS             3644..4594
                     /locus_tag="VFX94_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5769233.1"
                     /translation="MKRLLVLLCFVTCSTHAAYPEKPIRFVIPSAAGGSPDVLMRILT
                     QQLSAQMNVPIVVENKPGASFVLGTMDVVKAPPDGYSLGYGNIVSLAINRSLLGKVPY
                     DVEKDLTLVSNCVRVFNLLAVNNDLPVRTLAELIDYAKRNPGKLTMGSGGNGTTGHLG
                     GELFKAMTGTYIVHVPYKGSPQAINDLIAGNVQLMFDNLSSIGPHARSGRVRGIAVSG
                     PRRSPVFPELPTIAEAGVPGYETVAWGGVIGPARLPPEVVARLGKEIKAALAHPNVVE
                     RYRQLDTEPDGGSPEEFLALVRRETPKWAEVIRRSGAKVD"
     gene            4587..>4774
                     /locus_tag="VFX94_13450"
     CDS             4587..>4774
                     /locus_tag="VFX94_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX5769234.1"
                     /translation="MTDKLDLVIRNATVIDGTRAPRFGADIAVDAGRISGIGKIREKG
                     RSEIDAAGRIAAPGFIDA"
BASE COUNT          709 a         1561 c         1667 g          837 t
ORIGIN      
        1 tgatcggcgt gctgccgggg atcacgccgc tcgtggcgat cgggatgctc ttcccgctga
       61 cgttcacgct gccgccggtg tcctcgctca tcatgctcgc gggcatctac tacggcgcgc
      121 agtacggcgg ctcgaccacc gcggtgctcg tcaatctgcc gggcgagacc gccgcggcgg
      181 tgacgtgcat cgacggctac cagatggcgc gccaggggcg cgccgggccg gcgctcgcgg
      241 tggcggcgct cgcttccttc ttcgcggggt gcatcggcac gctgctcatc gcgctcgtcg
      301 ggccgccgct cggcgagtgg gcgctcaagt tcggcccctg ggaatactgc gcgctcatgc
      361 tgatgggtct cgtcgcttcg gcggtactcg cgcatggcga cgcggtgaaa ggcctggcga
      421 tggtggcgct ggggctgctc ttcggcatcg tcggcaccga cgtcaactcg ggcgtgccgc
      481 gcttcgcctt cgacctgccg cagctcttcg acggcatcgg cttcacggtg atcgcggtcg
      541 gtcttttcgc cgtggccgag atcgccgcca agctcgagtc gcgcgaggcg cgtgagctct
      601 tcaccggcgc gatcgcgcag ctcatgccca gccgccgcga cgtgggcgac gccgcctggc
      661 ccaccgtgcg cggcaccgcg ctcggcgcgg ccttcggtgt gctgcccggc accggccccg
      721 cgctctcctc gttcgccgcc tacatgctgg aaaagaagct cgcgcgcgac cccggccgct
      781 tcggccacgg cgcgatcgag ggcgtggccg cgcccgaggc ggcgaacaac gccgcggcgc
      841 agaccggctt catccccacg ctcacgctcg gcatcccggg gagcgcggtg atgacggtca
      901 tggtcggggc gatgatgatg cagggcttcg cgccggggcc gggggcgatg gcggagaagc
      961 ccgagctctt ctggggaatc atcgccagca tgtggatcgg gaacctgatg ctcgtggtgc
     1021 tcaacctgcc gctggtcggg ctgtgggtga agctgctgag cgtgccctat cgctggctct
     1081 tcccttcgat cgtcatgttc tgctgtatcg gcacctatag cgtgaccaac aactcgttcg
     1141 acgtgtacct ctgcgccgcg ttcggactgc tgggctacgt gcttgccaag ctcgagtgcg
     1201 agccggcgcc gctgctcctg ggctacgtgc tcgggccgct cctcgaggag catctgcgcc
     1261 gcgcgatgat cctcgggcgc ggcgatccgc tccagttcct cgcgcgcccg atcagcctcg
     1321 cgttcgtgct cggcaccgcg ctgctgctcg tgttgatggc ctggccggcg ctgcggcggg
     1381 gaaagaggct cgtcgggcgc ctcagccaat aagcccgtgc aggcgccggt cgagcgcgag
     1441 cagcagcgcg ttcactcccg cggcgaagac gacgagaagg agcgtgagcg cgaggatgcg
     1501 ctcgatgctg tgcaggctcg cctgcatgag gaggtggccg acgccgtgct gcgagccgaa
     1561 catctcgccg agcagcgtgc cgatgagcga gagcgagaag ccgatgcgta tgccggtcac
     1621 gatctcgggc agcgccgccg gcgggaggtt atcccgaaag cagccttccg gtcatcgcca
     1681 ggcgtgcgcg gaactcaggc gttccgggct gcaggtgcct gcgcgcccag gcgtcacgcg
     1741 tcgtcttcag cctgccgtcg tccttgttcg atccgggagc gaaggcgcgc gcgcggtagt
     1801 gctcgtagag gcgcaggaac tcggcgaggt acatgccggc gagccggcgg ctgcccagga
     1861 tctcgagcag gttctcgtcg ttgttgttca ccgagttctt gctcatgttg gccgagccgg
     1921 tgtagatgag cgggtgcggc gtctcggcgt cgatgatcac catcttgtgg tgcacgatca
     1981 ccggcgggaa gggcggccgc ggctcgcccg ggaagagctg ctgctcgtcg acgaagccca
     2041 ccggcttcgt gtcccagcgg tagcggctcg cctcgatcac gtcgcggttg tccttggagc
     2101 ggtggtagag ctcgatcgcc gctttttcgt cggcctgcat gtcgtcctcg acctgctcgc
     2161 cttccttgtc ggcgatgttg ttgaccagac cgaacatcat caggccccgg tcgccggcgg
     2221 cgaagcaggc gtggcgcagc gcctcgtcgg taggcgagaa gaggcagaac atcaccgagc
     2281 tgcgggcgtt gtggatcgcg cgcaccaccg tctcgatgga gtcgcgcctc ttcgccggct
     2341 cgggagagaa gaacacgcgg atcgcggcgt cgccgagcgt ctcggtcggc gaccagccgg
     2401 atttctttgc cgtctcgccc ttggtgagat ttgccttgag aaggtcgtag cggtccagat
     2461 aggactgggc gagccctggc gacttgaaaa gatggatcag gtttgcctgg gtggtgaggc
     2521 cgccggtagt gaagttcgcc gagcccatca gcacggactt cggcttcgaa tttgcgccct
     2581 cgaagccctt tcccgagacg atgaacttgt tgtgcatgat gttcgtcctg ttgcgcggat
     2641 ggaacctcac cttgtccttg agctcgacta ccgccttctc ggtcggcggc ggcatggtct
     2701 cgccgtgcgc gtccttgatc gtgcgcgcat cgtagacgag atcgatcgcc gcgcccgctt
     2761 cgctggattt cgccagcgcc gggatggtcc agagcttgtc ggtgaggtgg taaatcgcgc
     2821 ccgcgagttg gcgcttgccg ctgcctgcgc cctcgatgtg gtcgacgagc gcggtgtgca
     2881 tgccggcgct cagccagctg cgcagcttga gcgcctgctc gggcgtcggc tgctggttcg
     2941 cggtgagtcc catctcgcgc agcatgaacg agaacgcctg cgagctgacg acggcgcggt
     3001 tgaaccaggt gccgatgccg tccaccacgt gcggcgggat atcgacccgc agctcggcct
     3061 tcgcctcctc cagcagccgc acctggccgg gagcgccgca ggccggccac gcttcgtagc
     3121 ggatctgcct cacgtcgccg tgctcaggac cgatgcgcgc gtcccaccac atgaacttct
     3181 ggaacggcgc ctcgttcgac gggtagtcct taccttcctc cggtgcggcg aaacggcgtc
     3241 ggtttggcag ccaggaccgc gactggtggt agaagcccgg cgttcggcgg atcgagaagc
     3301 cgaggaaatc ggcgtgctgg tcggcgtcgg gccagtcgaa ggcgaggagg acgagcgccg
     3361 gggatgcgta gctgcgtatg acgatcgaag ccatggcgat tcctttcttg cgttacgccg
     3421 gaaaagcagc gtgcgccgat tgcggcggga ttactacgtg cgcaaggcgc accccgcgtg
     3481 tgcgagagtg gccaactatc ctggtctgcg caaggtcgcc ggactgtggc aggcgctcgc
     3541 gcggcgcact cgcgggaggc gccgggcgaa ggccgcggag cagcggctgg agccctggca
     3601 ttcagtagaa tcctcggggc tacccacccc gaggcatgaa gatatgaaac ggctgcttgt
     3661 cctgctttgc ttcgtcacgt gcagcacgca tgccgcttat cccgagaagc cgatccgctt
     3721 cgtgattccc tcggccgcgg gcggctcgcc cgacgtgctg atgcggatcc tcactcagca
     3781 gctctcggcg cagatgaacg tgccgatcgt ggtggagaac aagccgggcg cctcgttcgt
     3841 gctcgggacg atggacgtgg tgaaggcgcc gccggacggc tactcgctcg ggtacggcaa
     3901 cattgtgtcc ctcgcaatca accgctcgct gctcggcaag gtgccctacg atgtggagaa
     3961 ggatctgacg ctggtctcga actgcgtgcg cgtgttcaac ctgctcgcgg tgaacaacga
     4021 cctgccggtg cgcacgctcg ccgagctcat cgactatgcc aagcgcaatc ccggcaagct
     4081 caccatgggc tcgggtggaa acggcaccac cgggcacctg ggcggcgagc tcttcaaggc
     4141 gatgaccgga acctacatcg tccacgttcc ctacaagggc agcccgcagg cgatcaatga
     4201 cctcatcgcg ggcaacgtgc agctcatgtt cgataacctc tcctcgatcg ggccgcacgc
     4261 gaggagcggc cgcgtgcgcg gcatcgccgt gtccgggccg cggcgctcgc cggtgtttcc
     4321 cgagctgccg acgatcgccg aggcgggtgt gccgggctac gagaccgtcg cctggggcgg
     4381 cgtcatcggc ccggcgaggc tgccgcccga ggtcgtagcg aggctgggca aggaaatcaa
     4441 ggctgccctg gcacacccga acgtagtgga gcgctacagg cagctcgaca ccgagcccga
     4501 cggcggctcg ccggaagagt tcctggcgct cgtcaggcgc gaaacgccga agtgggcgga
     4561 agtcatccgc cgctcgggag ccaaggttga ctgacaagct cgacctcgtc atccgcaacg
     4621 cgaccgtgat cgacggcacg cgcgcgccac gatttggagc cgatatcgcc gtcgacgcgg
     4681 gaaggatctc cggcattgga aaaatcaggg aaaaaggcag aagcgaaatc gacgccgccg
     4741 gcaggatcgc cgcgcccggg ttcatcgacg cgca
//
LOCUS       DASUNT010000671         3285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1136682, whole genome shotgun sequence.
ACCESSION   DASUNT010000671 DASUNT010000000
VERSION     DASUNT010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136682"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VFX94_13455"
     CDS             <1..533
                     /locus_tag="VFX94_13455"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX5769235.1"
                     /translation="IDRAQPPPGGDPARYLAPTLAVPSVLGEISSLAREITAGAASDE
                     ERIARLIAWMDANIAKEAIDAFSAADVLRARRAECQGHSYLFAAFARALGVPARVVNG
                     IAYSEEHGGFLYHTWNEAWIAGHGWRPIDATFGQAHADATHVKLIEGETAAELVPLVN
                     LVGRVRVASVSALARW"
     gene            544..1095
                     /locus_tag="VFX94_13460"
     CDS             544..1095
                     /locus_tag="VFX94_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEX5769236.1"
                     /translation="MKFLFDFFPVILFFVAFKLADIYVATGVAIAATFVQVAWLKLRG
                     QRVEPMLWASLGIIVLFGGATLLLQDETFIKWKPTVLYWLFGAVLAGAALLKKNLIRS
                     VLSQEMQLPDPIWSRLNWSWIGFFLLMGALNLYVAYNYSTDHWVNFKLFGGMGLMLLF
                     VLVQALVLARFIEEKDERRGENP"
     gene            1103..1348
                     /locus_tag="VFX94_13465"
     CDS             1103..1348
                     /locus_tag="VFX94_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX5769237.1"
                     /translation="MALEPLDIELVDESSKHAGHAGWRPGGGTHWRLSIVSPRFAGKT
                     TLARHRMVYQALGELMQNPIHALAIVARSPEETKGSP"
     gene            1345..2181
                     /locus_tag="VFX94_13470"
     CDS             1345..2181
                     /locus_tag="VFX94_13470"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5769238.1"
                     /translation="MKTRILAVALLAPALALAQVPEKKDGKAPAAKSAPAKPGGGKPV
                     ATVNGVPVPQSRADFLMQQQLSRGGADTEQMRGMVRDELVNREILMQEAQKAGVAKQP
                     EVQTQLDMARQEIIVSAYLRDWVRKHPITDAEVQQEYEKAKAEQGDKEYKARHILVES
                     EDEAKALIAQLKKGGKFEDLATKNSKDGGSAQRGGELDWNVPAGYDKQFSDAMVKLEK
                     GKYTETPVRTRFGFHIIQLDDVRAAKFATLAEVKPRIQQKLAQSRIEELVRGLRAKAK
                     IE"
     gene            2191..2391
                     /locus_tag="VFX94_13475"
     CDS             2191..2391
                     /locus_tag="VFX94_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HEX5769239.1"
                     /translation="MVRKYGRKASQKVRQAMRERKRGTLRSGRSGKKVKSRKQAIAIG
                     LSQARRAGAKVPRKRKSSRRKK"
     gene            2418..2897
                     /locus_tag="VFX94_13480"
     CDS             2418..2897
                     /locus_tag="VFX94_13480"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX5769240.1"
                     /translation="MRGSLLDEPVDAIVNAANGHLAHGGGVAGIISRAAGPELQAESD
                     ALIRKLGPLPAGSAVVTAAGKLPFKGVIHAVGPRRGEGDEEAKLLQALSEAFKRAQER
                     GWASVSFPAVSSGIFAVPLDVCARAYVKAARASPLRNVRLCLRDQPVIDAVMKELSR"
     gene            2894..>3285
                     /gene="msrA"
                     /locus_tag="VFX94_13485"
     CDS             2894..>3285
                     /gene="msrA"
                     /locus_tag="VFX94_13485"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX5769241.1"
                     /translation="MRSVAFGLILALAAPALAQQQKPEKATFAGGCFWCTEEAFEKVP
                     GVTSVVSGYMGGKLKNPTYDQVSSGRTGHTEVVEVTYDPAKVSYEKLLDTFWLNHDPT
                     VKDRQFCDSGSQYRPEIFYHTEEQKRLA"
BASE COUNT          613 a         1053 c         1108 g          511 t
ORIGIN      
        1 gcatcgaccg cgcacagccg ccccccggcg gcgatcctgc gcgctacctt gcgccgacgc
       61 tcgcggtgcc gagcgtgctc ggcgagatct cctcgcttgc gcgcgagatc accgccggcg
      121 cggcgagcga cgaggagcgc atcgcgcgcc tgatcgcgtg gatggacgcg aacatcgcca
      181 aggaggcgat cgacgcgttc agcgctgccg atgtactgcg cgcgcggcgc gccgagtgcc
      241 aggggcattc ctatctcttc gccgcgttcg cacgcgcgct cggcgtgccg gcgcgcgtcg
      301 tgaacggcat cgcctactcc gaggagcacg gcggcttcct gtaccacacg tggaacgagg
      361 cttggatcgc gggccacggc tggcgaccga tcgatgcgac gttcggccag gcgcacgccg
      421 acgccacgca cgtcaagctg atcgagggcg agaccgcggc cgagctggtg ccgctcgtca
      481 acctcgtcgg gcgcgtgcgc gttgcgtcgg tgtcggcgct cgcacgctgg taaactcggc
      541 taaatgaaat tcctgttcga cttcttcccg gtgatccttt tcttcgtcgc gttcaagctc
      601 gccgacatct acgtcgcgac cggggtggcg atcgctgcga ccttcgtcca ggtggcgtgg
      661 ctcaagctgc gcggccagcg cgtcgagcca atgctgtggg cgagcctcgg cattatcgtg
      721 ctgttcggcg gcgcgacgct gctcttgcag gacgagacgt tcatcaagtg gaagccgacc
      781 gtgctctact ggctgttcgg cgcggtgctg gccggcgcgg cgctgctgaa aaagaacctg
      841 atccgcagcg tgctgtcgca ggagatgcag ctgcccgacc cgatctggag ccggctcaac
      901 tggagctgga tcgggttctt cctcctcatg ggcgcgctga atctgtacgt ggcttacaac
      961 tattcgaccg accattgggt caatttcaag ctcttcggcg gcatggggct gatgctgctc
     1021 ttcgtgctcg tccaggcgct cgtgctcgcc cgcttcatcg aagagaagga tgagcgtcgc
     1081 ggcgagaatc cgtgagcgcc tcgtggcgct cgaaccgctg gacatcgagc tcgtcgacga
     1141 gagctcgaag cacgccggcc atgccggctg gcgtcccggc ggcggaactc actggcggct
     1201 cagcatcgtg tcgccgcgct tcgccgggaa gacgacgctc gcgcgccacc gcatggtgta
     1261 ccaggcgctg ggcgagctga tgcagaatcc catccacgcg ctcgcgatcg tcgcgcgcag
     1321 ccccgaagaa acgaaaggat ccccgtgaag actcgaatcc tcgccgtagc gcttcttgct
     1381 cccgccctgg cgctcgcgca ggtgccggaa aagaaggacg ggaaggcccc cgcggcaaag
     1441 tccgccccgg ccaagcccgg gggtgggaag cccgtcgcca ccgtgaacgg cgtgcctgtc
     1501 cctcagtcgc gcgccgactt cctgatgcag cagcagctga gccgtggcgg cgccgatacc
     1561 gagcagatgc gcggaatggt gcgcgacgag cttgtgaacc gcgagatcct gatgcaggag
     1621 gcgcaaaagg cgggcgtcgc caagcagccc gaggtgcaga cacaactgga catggcccgt
     1681 caggaaatca tcgtcagcgc ctatctgcgc gactgggtgc gcaagcaccc gatcaccgac
     1741 gccgaagtgc agcaggaata cgaaaaagcc aaggcagagc agggcgacaa ggaatacaag
     1801 gcgcgccaca tcctggtcga gagcgaggac gaagccaagg cgctgatcgc gcagctcaag
     1861 aaaggcggca agttcgagga cctggcgacg aagaactcca aggacggcgg tagcgcccag
     1921 cgcggcggcg agctcgactg gaacgtgccc gccggctacg acaagcagtt ctccgacgcg
     1981 atggtgaagc tcgagaaagg caagtacacg gaaacgccgg tgcgtacgcg cttcggcttc
     2041 cacatcattc agctcgacga cgtgcgcgcg gccaagttcg cgaccctggc cgaggtgaag
     2101 ccgcggatcc agcagaagct ggcgcaaagc aggatcgaag agctggtacg ggggctaaga
     2161 gcaaaagcaa agatcgagta ggagctcttt atggtccgga aatacggcag gaaggcatcc
     2221 cagaaagtcc gccaggcgat gcgcgaacgt aagcggggca cgctgcgcag cgggcgctcc
     2281 ggaaagaagg tgaagagccg caagcaggcg atcgcaatcg gcctctcgca ggcgcgccgc
     2341 gccggcgcga aagtgccacg caagcgcaag tcctcgcgca ggaagaagta ggctcttccg
     2401 gccagacgtt cctcgtcgtg cggggcagcc tgctcgatga gccggtggat gccatcgtca
     2461 acgcggcgaa cggccacctc gcccatggcg gcggggtggc cggaatcatc tcgcgtgccg
     2521 ccggcccgga attgcaggcc gaaagcgatg cgcttatccg caagcttggc cccctgcccg
     2581 cgggctccgc ggtcgtcacg gcagccggta agctgccttt caagggcgtg atccatgccg
     2641 tcggaccgcg ccggggggaa ggcgacgagg aagccaagct tcttcaggct ttaagcgaag
     2701 ctttcaagcg ggcccaggag cggggctggg cgtcggtttc cttcccggcg gtgtcgagcg
     2761 gtatcttcgc ggtgccgctc gacgtctgcg cccgcgccta tgtaaaggcg gcgcgcgcct
     2821 cccctttgcg taatgtccgg ctatgcttgc gcgaccaacc ggtcatcgat gccgtcatga
     2881 aggagctatc cagatgagaa gcgtcgcctt cgggctgatc ctggcgcttg cagcgccggc
     2941 tctcgcgcag caacagaagc ccgaaaaggc gacattcgcc ggcggctgct tctggtgcac
     3001 tgaggaagcg ttcgaaaagg tgcccggagt gacctccgtg gtgtccggct acatgggagg
     3061 aaagctaaag aaccccacgt acgaccaggt ttcgagcggc cgcaccgggc acacggaggt
     3121 cgtcgaggtg acctacgacc ctgcgaaggt gagctacgaa aagctgctcg atacgttctg
     3181 gctcaaccac gacccgaccg tcaaggatcg gcagttctgc gactcgggct cccaataccg
     3241 gcccgagatc ttctatcaca ccgaggagca gaagcgcctc gccga
//
LOCUS       DASUNT010000672         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_436733, whole genome shotgun sequence.
ACCESSION   DASUNT010000672 DASUNT010000000
VERSION     DASUNT010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436733"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VFX94_13490"
     CDS             complement(<1..593)
                     /locus_tag="VFX94_13490"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5769242.1"
                     /translation="MKRAFLLLLALAAFDAPAQGWPAKPIRAIVPVGAGSSTDIVHRL
                     VLEQLASQLGQQVVVENRVGAGGTIGAAFVAKSAPDGYTLLAHGSAHTIAPALYKGLS
                     YDPARDFAAIAPIGISPSVLVVSPAKGMKTAAELVAAAKARPNALNFSSVGIGTATHL
                     SAERFMSSAGVQAVHIPFKGGAEAMTEVIAGRVDFFFG"
     gene            698..1648
                     /locus_tag="VFX94_13495"
     CDS             698..1648
                     /locus_tag="VFX94_13495"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5769243.1"
                     /translation="MDLRQVRTFVTVAEVGTVCKAAERLHVAQPALSRQIAHLEDELG
                     LKLFDRVGRRLVLTSEGEQLLNDCRGLLNYARALGEQADVLRRGDVGVLRVSASPHLI
                     EGIFPEFLRHYAARYPNVQVRLVDAAAGAEGFAMLERGEIHLMQTIVRAMAPDEQRFA
                     SHPLAPMEMLAACHPKLGLGTDGAVEIGRLAPYPLLQATPDFAMRRNFDAACRLAGFT
                     PNNVLESRAPHALLAMAEAGHGVAVIPSALRIHRYRVRVLRVTYRGKPLDEPLTVLSD
                     KRRPLPGYAKVFCEMLAQHVRKGFPLPREFHEKPPRRRRA"
     gene            1719..>2630
                     /locus_tag="VFX94_13500"
     CDS             1719..>2630
                     /locus_tag="VFX94_13500"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEX5769244.1"
                     /translation="MSPNLLRAAASCLLLAVAPSRPAETQDPHRFRASSLKPPLTAEI
                     ETQLGPLRFKGAVSVTRTEGRFFGWSGFWVSPDGSRFAAVDGGDWAQGSLSYDADGNL
                     ASLTVQATGPLLDDKGEPFTHQDDKDAEALDFDGTRFLVGFETHDRVLAYRDFTSAAE
                     RIELPEEALVGIPRGAGFSSVAHLPNGATIVLPEYTADDPQEMRVSTRTRGWLLTKNG
                     AGPVWLRKARGLPVSLSAFPNGDLLLLEVLPNRVKITDTRLSRISAKDIAIGGTMRPK
                     ELATLRPPLVDGRFEGSAVRRGPRGETL"
BASE COUNT          454 a          859 c          863 g          454 t
ORIGIN      
        1 ccgaagaaaa aatccacacg gccggcgatc acttcggtca tcgcctcggc cccgcccttg
       61 aacgggatat ggaccgcctg caccccggcg ctcgacatga agcgctcggc gctcaggtgc
      121 gtagccgtgc cgatgccgac cgaagagaag ttgagcgcat tcggccgcgc cttcgccgca
      181 gcgacgagct cggcagccgt cttcatgccc ttcgcggggg aaacgacgag cacgctcggc
      241 gaaattccga tcggcgcgat ggcggcgaag tcgcgcgccg ggtcgtagga cagccccttg
      301 tacagcgccg gggcgatggt gtgcgccgag ccgtgcgcaa gcagggtgta gccgtcaggg
      361 gccgacttcg ccacgaaggc ggctccgatc gtgccgccag cgccgacacg gttctccacc
      421 acgacctgct gtccgagctg cgacgcaagc tgctcgagga cgagccggtg cacgatgtcg
      481 gtcgagctgc cggcgccgac cggcacgatg gcccggatcg gtttcgccgg ccagccctgg
      541 gccggggcgt caaatgcggc aagcgcgagc aggagcagga aagcgcgttt catggtcacc
      601 tccgtcaagt gccggggcat tctggaaatg ggcgcctcat aagtccaata tatagtttgt
      661 ctaggtcgtc atgccgggcg ggcattaaaa ggaaaccatg gacctgcgcc aagtccgcac
      721 cttcgttacc gtcgccgagg tgggcacggt gtgcaaggcc gccgagcgct tacacgtcgc
      781 gcagccggcg ctctcgcggc agatcgccca tctggaggac gagctcggtc tgaagctgtt
      841 cgaccgcgtc ggccggcggt tggtgctcac gagcgaaggc gagcagctgc tcaacgattg
      901 ccgcggcctg ctcaactacg cccgggcgct aggcgagcag gccgacgtcc tgcggcgcgg
      961 cgacgtcggc gtgctgcgcg tgtcggcctc gccgcacctc atcgagggca tcttccccga
     1021 attcctgcgc cactacgcgg cgcgctatcc caacgtgcag gtcaggctgg tggatgcggc
     1081 ggcaggcgcg gaggggttcg ccatgctcga gcgcggcgag atccatctca tgcaaacgat
     1141 cgtgcgtgcg atggccccgg atgagcagcg cttcgcgagc cacccgctcg cgcccatgga
     1201 gatgctggcg gcgtgccatc cgaagcttgg cctgggcacc gacggtgcgg tcgagatcgg
     1261 ccgcctcgcg ccctacccgc tcctgcaagc gacgccggac ttcgccatgc ggaggaattt
     1321 cgacgccgcc tgccggctcg ccggcttcac gccgaacaac gtgctcgaat cccgcgcccc
     1381 gcatgcgctg cttgccatgg ccgaagcggg ccacggggtc gccgtcatcc cgtcggctct
     1441 gcgcatccac cgctaccgcg tgcgggtgct gcgcgtcacc taccgcggca agccactgga
     1501 cgagccgctg accgtgctat ccgacaagcg ccgcccgcta cccggttatg ccaaggtgtt
     1561 ctgcgagatg ctcgcgcagc acgtgcgcaa gggattcccg ctgccacggg aattccacga
     1621 aaaaccgcct cgaaggcgac gggcgtaggc tttaaacatg cagcctaaca aggcgagcgc
     1681 ccagtttggc gatattcctc ggaagggagg acaacgtcat gtcaccgaac cttcttcggg
     1741 ccgccgcctc atgcttgctt ttggcagtgg caccgagtcg tcctgcggag acccaggacc
     1801 cgcaccggtt tcgagcttcg tccctcaagc caccgctcac ggcggaaatc gaaacgcagc
     1861 taggaccgct tcgcttcaag ggggcggtta gcgtaacgcg caccgaaggg aggttcttcg
     1921 gctggtccgg cttctgggtt tcgcctgacg gaagtcggtt tgcggctgtc gacggcgggg
     1981 actgggcgca aggatctctc tcctatgacg ccgacggaaa tcttgcttct ctcacggtgc
     2041 aagcgaccgg cccgctgctt gacgataagg gagagccctt tactcatcag gatgacaagg
     2101 atgccgaagc gctcgacttt gacggaacgc gcttcctcgt gggctttgaa acgcatgatc
     2161 gcgtgcttgc ctacagggac ttcacttcag ccgccgagcg catcgagctg cccgaggaag
     2221 cgctggtcgg cattccgcgt ggcgccggtt tttcatcggt cgcacactta ccgaatggcg
     2281 caacgatcgt actccccgag tacaccgctg acgatcctca ggaaatgagg gtctccacgc
     2341 gaacgcgcgg ctggctccta acgaagaatg gcgcgggccc ggtctggctc cgcaaggctc
     2401 gtgggctacc ggtttcattg tcggcatttc caaatggcga cctgttgctc ctggaagtct
     2461 tgccgaatcg cgtaaagatt acagatacgc ggttgagcag gatttcggcg aaggacattg
     2521 cgattggcgg aacgatgcgg ccaaaggagc tcgcgaccct ccgccctcct ctggtggacg
     2581 ggcgcttcga gggttccgcc gttcgtcgag gtccgcgtgg agagacgttg
//
LOCUS       DASUNT010000673         2458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1224832, whole genome shotgun sequence.
ACCESSION   DASUNT010000673 DASUNT010000000
VERSION     DASUNT010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224832"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..>2458)
                     /locus_tag="VFX94_13505"
     CDS             complement(37..>2458)
                     /locus_tag="VFX94_13505"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5769245.1"
                     /translation="EFEEIIVRAAKEGVAIVRVKDIGRVELAKRDYSIASRLNGKTAT
                     TIAIYQQPGANAVLTADAVKKRMEELKAQFPSGLDYKIVLDTSLFTLYSIDKVVHTFF
                     EAVVLVVLVVFLFLQSLRATVIPILAVPVSIIGTFIGMHLLGFSINMLTMFGMILAIG
                     LVVDDAIVVVETVEANITTHKLSALEAAKRAMSEIAGALISIVLVLLAVFLPVAFLGG
                     VTGTLYKQFAITIAISMVISGIMALTLSPALAAIIIKAHHGDKNRFFRAFEAGFERVR
                     RSYLAGVSRLIAAWPLALAAFAAVIAGIVLMFRIVPSSFVPDEDQGYFFVVVRAPDSA
                     SLAVVERLTRQAEKIMLADPAVQDVDVVDGYDLIDGQFTNNGAVLFASLKPFGERKDP
                     SLLSFPTLGRLNAQFAGIREGFLFALNPPSIPGLGTTGGFELYLQNRGAGDPRVTGAA
                     VQAFLEKARSRPELAGVSSTFRAASQQLFVNLDRNKAEVLGVPVSDVFQTMQAFFGSQ
                     IAGQFSQFSRVWWVILQADADYRAQPDDFDKVYVRSRSGANVPLSALITVKYVASPKL
                     VTRFNGFPAAKITGSQAPGYSSGDALRAMEEVAQEALPADFSSAWAGQALQERQAGST
                     SSSAFIFGLIIVFLLLAAQFEKWTLPVGVVLSVPFAVLGALLLTWALGLENDVYFQVG
                     LVTLVGLAAKNAILISEFAIERVRHGMPAREAAVEAAGLRLRAIVMTSFAFVLGCVPL
                     AIATGPGANSLRAIGTGVIGGMLASTLVAVFFVPLFFWLLESMSRKKPAAAGGPAPQA
                     AHAPRKED"
BASE COUNT          456 a          818 c          837 g          347 t
ORIGIN      
        1 aaaagaaacg caactgaaaa cagcaaacct cgcaactcaa tcctccttgc gcggcgcatg
       61 ggcggcctgg ggcgcgggcc ctccagcggc agcgggcttc ttccggctca tcgactccag
      121 gagccagaag aaaaggggca cgaagaagac cgcgacgagc gtcgaggcga gcatgccgcc
      181 gatcacgccg gtgccgatcg cgcgcaggct gttggcgccc gggccggtgg cgatcgcgag
      241 cggcacgcag ccgaggacaa aggcaaacga ggtcatgacg atcgcgcgca gccgcaggcc
      301 cgccgcctcg accgcggctt cgcgcgccgg catgccgtgg cgcacgcgct cgatcgcaaa
      361 ctcggagatg aggatggcgt tcttcgccgc gaggccgacc agggtcacga ggccgacctg
      421 gaagtagaca tcgttctcga ggccgagggc ccacgtgagg agcagcgcgc cgagcaccgc
      481 gaagggaacc gacagcacca ccccgacggg cagcgtccac ttctcgaact gcgccgcgag
      541 cagcaggaag acgatgatca ggccgaagat gaacgcggac gaagaagtgc tgcccgcctg
      601 cctctcctgc agcgcctgtc cggcccatgc ggacgagaaa tcggccggca gcgcttcctg
      661 cgccacttcc tccatcgcgc gcagggcgtc gcccgagctg tagcccggcg cctggctgcc
      721 ggtgatcttc gccgccggga agccgttgaa gcgcgtgacg agcttcggcg aggcgacgta
      781 cttcaccgtg atgagcgcgg agagcggcac gttggcgccg cttcgcgagc gcacgtagac
      841 cttgtcgaaa tcgtccggct gcgcgcggta gtcggcgtcg gcctgcagga tcacccacca
      901 gacgcggctg aactgcgaga actggccggc gatctgcgag ccgaagaagg cctgcatggt
      961 ctggaaaacg tcggacaccg gcacgcccag cacctcggcc ttgttgcggt cgaggttgac
     1021 gaagagctgc tggctcgcgg cgcggaaggt actcgacacg ccggcgagct cgggacggct
     1081 ccgcgccttc tcgaggaagg cttgtaccgc cgcgccggtt acgcgcggat ctcccgcgcc
     1141 gcggttctgg aggtagagct cgaagccgcc ggtggtgccc aggcccggga tcgacggcgg
     1201 gttcagggcg aaaaggaagc cctccctgat gcccgcgaac tgggcgttca gccgcccgag
     1261 cgtgggaaag gagagcagcg acggatcctt gcgctcgccg aaaggcttga gcgaggcgaa
     1321 gagcaccgcg ccgttgttgg tgaactggcc gtcgatcagg tcgtagccgt ccaccacgtc
     1381 cacgtcctgc accgccggat cggcgagcat gattttttcg gcctggcgcg tcagccgctc
     1441 gaccacggcg aggctggccg agtcgggcgc gcgcaccacg acgaagaagt agccctggtc
     1501 ctcgtcgggc acgaagctcg agggcacgat cctgaacatc agcacgatgc cggcgatcac
     1561 cgcggcgaac gcggcgagcg cgagcggcca ggcggcgatc agcctcgaaa cgcccgcgag
     1621 gtaggaccgg cgcacgcgct cgaagccggc ctcgaaggcc cggaagaagc ggttcttgtc
     1681 gccgtggtgc gccttgatga tgatcgcggc gagcgccggc gagagcgtga gcgccatgat
     1741 ccccgagatc accatcgaga tcgcgatcgt gatggcgaac tgcttgtaga gcgtcccggt
     1801 cacgccgccg aggaacgcca ccggcaggaa caccgcgagc agcacgagca cgatcgagat
     1861 cagcgcgccg gcgatctcgc tcatggcgcg cttcgccgcc tcgagcgccg agagcttgtg
     1921 cgtggtgatg ttcgcctcga ccgtctccac caccacgatc gcgtcgtcca ccaccaggcc
     1981 gatcgcgagg atcatgccga acatcgtcag catgttgatc gagaacccca gcaggtgcat
     2041 gccgatgaac gtgccgatga tcgagaccgg caccgccagg atcgggatta cggtggcgcg
     2101 cagcgactgg aggaaaagga acaccaccag caccaccagc acgaccgcct cgaagaaagt
     2161 gtgcaccacc ttgtcgatcg agtagagcgt gaagagactc gtgtcgagga cgatcttgta
     2221 gtcgaggccg ctcgggaact gcgccttgag ctcctccatg cgcttcttca ccgcatccgc
     2281 cgtcagcacg gcgttcgcgc cgggctgctg gtagatggcg atggtcgtcg ccgtcttgcc
     2341 gttcaggcgg ctcgcgatcg agtagtcgcg cttcgcgagc tcgacgcgcc cgatgtcctt
     2401 gacgcgcacg atggcgacgc cctccttcgc cgcgcgcacg atgatctcct cgaactcc
//
LOCUS       DASUNT010000674         5041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1136775, whole genome shotgun sequence.
ACCESSION   DASUNT010000674 DASUNT010000000
VERSION     DASUNT010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136775"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VFX94_13510"
     CDS             <1..591
                     /locus_tag="VFX94_13510"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX5769246.1"
                     /translation="TILPNTAGCYSAQDAVRTLRLARELLDGHDLVKLEVLGDSHSLY
                     PNMPETLDAAKQLVKEGFKVMVYCSDDPIQAQMLEAAGCVAIMPLASLIGSGMGILNP
                     WNLQLVIDKVKVPVIVDAGVGTASDASIAMELGCDGVLMNTAVAAARDPVLMAKAMKK
                     AVEAGREAFLAGRMPKKLYAASPSSPTEGKIAPKAA"
     gene            588..1238
                     /gene="trmB"
                     /locus_tag="VFX94_13515"
     CDS             588..1238
                     /gene="trmB"
                     /locus_tag="VFX94_13515"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEX5769247.1"
                     /translation="MNRAIRSYVLRQGRTTPAQARALETLFPKYGIVFSHEPLGSRRG
                     ASLVLEIGSGMGETTVAIAKALPEVDFIAVEVHGPGVGSLLNAIEKEKLSNLRVIRHD
                     ALEVLEHMIGERSLAAIHLFFPDPWPKKRHHKRRLVQPAFAALAARKLAPGGVLHVAT
                     DWPDYAAWMEEVFLREPLLEPAAAGFTVRPVTKFESRGRRLGHPVRDLVFRRKERG"
     gene            complement(1144..2382)
                     /gene="hemW"
                     /locus_tag="VFX94_13520"
     CDS             complement(1144..2382)
                     /gene="hemW"
                     /locus_tag="VFX94_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX5769248.1"
                     /translation="MKPVVVETPAKVQLQALPPLALYVHIPWCVRKCPYCDFNSHERG
                     GALPENEYVARLMLDLESLLPSVWGRRVVSVFIGGGTPSLFSPQAIEALLSGVRARLP
                     LEPGAEVTLEANPGTVEAARFRGFRDAGVNRISVGVQSFEPRMLAALGRIHGAEEARR
                     AIEAAQQSCENVNIDLMYGLPGQTLAMARADIEEAAASGVPHVSAYQLTIEPNTVFWR
                     RPPPLPEHDECADMQVMIEDALGGRGFEHYETSAFARTGRRCQHNLNYWRFGDYLGVG
                     AGAHGKISFPDRVTRHEQARQPAEYLEGASRKDSTVPARELPFEFMLNALRLMEGFET
                     ALFSERTGLPLAAIAVPLEQAAGRGLIEPDPRRVRPSARGQRFLNELLTLFLSDGTPG
                     RAPDGRGGGRVIRISSPAGP"
     gene            complement(2363..2962)
                     /gene="rdgB"
                     /locus_tag="VFX94_13525"
     CDS             complement(2363..2962)
                     /gene="rdgB"
                     /locus_tag="VFX94_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX5769249.1"
                     /translation="MKLALASSNPGKVREIEALLEPLRIEVTPQRQLGIPDAEEPHAT
                     FLENALAKARHASRASGMPALADDSGLCVESLGGEPGVHSAYYAGREGSREERDARNN
                     AKLLSRMGEERTAYYCCVLVMVRRPEDPRPLVAEGIWRGEIARIPRGSNGFGYDPLFY
                     LKDLGKTAAELDPDYKNRVSHRGLALRKLLELLRETGSR"
     gene            complement(2959..3684)
                     /gene="rph"
                     /locus_tag="VFX94_13530"
     CDS             complement(2959..3684)
                     /gene="rph"
                     /locus_tag="VFX94_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX5769250.1"
                     /translation="MGVERSAGRRADELRPVRIERRYTRHAEGSVLVEFGDTRVLCTA
                     SVDERVPPFLKDSGRGWVTAEYGMLPRSTNTRAEREAARGRQSGRTQEIQRLIGRALR
                     AVMDLRGLGQRTIQIDCDVLQADGGTRTAAITGSFVALHDAVSWLKEKNVVVQNPIRD
                     HVAAVSVGMAGGSALLDLDYAEDSACGCDMNVVMTGAGRLVEVQGTAERDTFSRAELD
                     RLLDLAAQGIAQLVRHQKISLGL"
     gene            complement(3675..4412)
                     /locus_tag="VFX94_13535"
     CDS             complement(3675..4412)
                     /locus_tag="VFX94_13535"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEX5769251.1"
                     /translation="MKQPIIVQESRIGAREKNEDRVAHWSSSASVAMAVADGLGGHAH
                     GELAAQVVVDVVGASYEAEARPLVADPAGFLVRSLAAGHAAIVREAEKRDLIDHPRTV
                     IAACVVQEGRVHWTHVGDCRFYLLRGGEVIARSRDHTVVQQLVDEGRIPQELAGMHPL
                     RNRLLQCLGGYETPRIGPVESASLASRDVVLLCSDGFWGPLPEREVVKALLSRPLDQA
                     IGELVAAAESGAGFDCDNISVVALRWE"
     gene            4480..>5041
                     /locus_tag="VFX94_13540"
     CDS             4480..>5041
                     /locus_tag="VFX94_13540"
                     /inference="COORDINATES: protein motif:HMM:NF015699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX5769252.1"
                     /translation="MSLPVQSMTGYAAAAAGFARGTLGLELRSVNSRFLDLQVRIADE
                     LRAHEPLLRELIAARISRGKVDCRLSLAESTGQTASRLNAEAISRLRSLADAARGAFP
                     EAAPLRVADILRWPGVIAEIGDDESLRATVAGLCRRALDELVATRTREGEKLAATVLE
                     RVHAMHARLGEIAPLMPQAIAAFQEKL"
BASE COUNT          791 a         1753 c         1674 g          823 t
ORIGIN      
        1 acgatcctgc cgaacacggc aggctgctac agcgcgcagg atgcagtgcg cacgctgcgc
       61 cttgcgcgcg agctgctcga cgggcacgac ctcgtcaagc tcgaagtgct gggtgactcg
      121 cattcgctct accccaacat gccggaaacg ctcgacgcgg cgaagcagct cgtcaaggaa
      181 ggcttcaagg tcatggtgta ctgctcggac gaccccatcc aggcccagat gctcgaagcc
      241 gccggctgcg tggcgatcat gccgctcgcc tcgctgatcg gctcgggcat gggcatcctg
      301 aacccctgga acctgcagct cgtgatcgac aaggtgaaag tcccggtcat cgtcgacgcc
      361 ggagtcggca ctgcgtcgga cgcatcgatt gccatggagc tcggctgcga cggcgtgctg
      421 atgaacacgg cggtcgctgc ggcacgcgat ccagtgctga tggcgaaggc gatgaagaag
      481 gcagtcgagg caggccgcga ggcgtttctt gccggccgca tgccgaagaa gctgtatgcg
      541 gcttcgccga gctctcccac cgaaggaaag atcgcaccga aagccgcttg aaccgggcga
      601 tccgcagcta cgttctgcgg caaggcagga ccactcctgc gcaagcccgc gcgcttgaaa
      661 cgctttttcc caagtacggc attgtgttct cgcacgagcc gctcggctcg cggcgcggcg
      721 cgtcgctcgt tctcgagatc ggctcgggca tgggcgaaac caccgtggcc attgccaaag
      781 cgctccccga ggtggacttc atcgcggtgg aagtacacgg acccggagtc ggcagccttc
      841 tgaacgcaat cgagaaggaa aagctttcca acctgcgggt catccgacac gacgcgctcg
      901 aggtgctcga gcacatgatc ggcgagcgct cgctcgccgc catccatctt ttctttcccg
      961 acccgtggcc caagaaacgt caccataaac gccgcctggt gcagcccgcg ttcgcggcgc
     1021 tcgccgcaag gaagcttgcg cccggcgggg tgctgcacgt agcgaccgac tggccggact
     1081 atgcggcgtg gatggaggaa gtcttcttgc gcgagccgct gctcgagccg gcggccgccg
     1141 gcttcacggt ccggccggtg acgaaattcg aatcacgcgg ccgccgcctc ggccatccgg
     1201 tgcgcgacct ggtgttccgt cggaaagaaa gagggtgagc agctcgttga ggaagcgctg
     1261 gccgcgtgcg ctcggccgca cgcgccgcgg gtcgggctcg atcaggccgc gccctgccgc
     1321 ctgctcgagc ggcacggcga tggcggcaag cggcaagccg gtccgttcgg agaacaaagc
     1381 ggtttcgaag ccttccatca ggcgaagcgc gttcagcatg aactcgaacg gcagctcgcg
     1441 tgccgggacg gtgctgtcct tgcgcgatgc accttcgagg tactccgccg gctgcctcgc
     1501 ctgctcgtgg cgcgtaacac ggtcggggaa gctgatcttg ccgtgcgcgc ctgcgccgac
     1561 tccgaggtag tcgccaaacc gccagtagtt caggttgtgc tggcagcgcc ggcccgtcct
     1621 ggcgaatgcc gaggtttcgt agtgctcaaa acctctcccg cccagcgcat cttcaatcat
     1681 cacttgcatg tcggcgcact cgtcgtgctc gggaagcggc ggcggccggc gccagaatac
     1741 cgtgttcggc tcgatcgtca gctggtaggc cgagacgtgc ggcacgccgc tcgccgctgc
     1801 ctcctcgatg tccgcacgcg ccatggcaag cgtctgcccc ggcaacccgt acatgagatc
     1861 gatgttgacg ttctcgcagc tttgctgcgc cgcttcgatg gcgcgccgcg cctcctccgc
     1921 gccgtggatg cgtcccagcg ccgcaagcat gcgcggctcg aagctctgca cgccgaccga
     1981 aatccggttc acccccgcat cgcgaaagcc gcggaagcgc gccgcctcga ccgtgcccgg
     2041 gttcgcttcg agcgtgacct ccgcgcccgg ctcgagcggc aggcgcgcgc gcacgccgga
     2101 aagcagcgct tcgatcgcct gcggggaaaa caggctcggc gttccgccgc cgatgaacac
     2161 gctcacgact cggcgccccc acacggaggg aagcaggctt tcgaggtcca gcatgagccg
     2221 cgcgacgtat tcgttctccg gtagtgcgcc gccgcgctcg tgcgagttga agtcgcagta
     2281 agggcattta cgcacgcacc aggggatgtg tacgtagagc gcaagcggcg gcagggcctg
     2341 cagctgcacc ttcgcgggag tttcaacgac taccggtttc acgcagtaac tccagcagct
     2401 tgcgcaatgc caggccgcgg tggctgacgc ggttcttgta gtccgggtcg agctcggccg
     2461 ccgtcttccc gaggtccttc agatagaaga gcgggtcata gccgaagcca ttcgacccgc
     2521 gcgggatgcg cgcgatctcg ccgcgccaga tcccttcggc caccagcggg cgcggatctt
     2581 ccggacgacg caccatcacc aggacgcagc agtaataggc cgttcgctct tctcccatcc
     2641 tggaaagaag cttggcgttg tttctggcat cgcgctcctc gcggctgccc tcgcgcccgg
     2701 cgtagtacgc cgagtgcacg cccggctcgc cgcccagcga ttccacgcaa aggcccgagt
     2761 cgtcggcgag cgccggcatc cccgatgcgc ggctcgcgtg gcgcgccttg gcgagcgcgt
     2821 tctcgagaaa ggttgcgtgc ggttcctcgg cgtcgggaat gccgagctga cgttgcgggg
     2881 tgacttcaat tcgcagaggc tcgagcagcg cttcgatctc ccgcaccttg cccggattgg
     2941 acgaggcgag cgcgagcttc acaagccgag cgaaatcttc tggtgccgga cgagttgcgc
     3001 gatgccctgg gctgcgagat cgagcagcct atcgagctcg gcgcgcgaga aagtgtcgcg
     3061 ctcggccgtg ccctgcactt ccaccaagcg tcccgccccc gtcatcacga cgttcatgtc
     3121 gcagccgcag gcggagtctt ccgcgtagtc gaggtcgagg agcgcgctcc cgcccgccat
     3181 gcccaccgac acggcggcga cgtggtcgcg gatagggttt tgcacgacga catttttctc
     3241 tttcagccat gaaaccgcgt cgtgcagggc cacgaacgag cccgtgatcg ccgcggtgcg
     3301 ggtaccgcca tcggcctgca gcacgtcgca gtcgatctgg atcgtcctct gcccaagccc
     3361 gcgcaggtcc attaccgcgc gcagcgcgcg gccgatgagg cgctggatct cctgcgtgcg
     3421 gccagactgc ctgccgcgcg ccgcctcgcg ctcggcgcgc gtattcgtcg agcgcggaag
     3481 catgccgtat tcggcggtta cccagccgcg gccgctgtcc ttcaggaatg gcggcacgcg
     3541 ctcgtccacg ctcgccgtgc acagcacgcg ggtatcgccg aattccacga gcacggagcc
     3601 ctcggcatgc cgcgtgtagc ggcgctcgat gcgcaccggc cgaagctcgt ctgcgcgccg
     3661 gccggcgctt ctttctactc ccatcgcaaa gccaccacgg agatgttgtc gcaatcgaac
     3721 ccggcgccgg attcggctgc cgcaacgagc tcgccgatcg cctgatcgag cggacgggaa
     3781 agcagcgctt tcacgacctc gcgctccggc agcgggcccc agaaaccgtc ggagcagagc
     3841 agcaccacgt cgcggctcgc gagcgatgcg ctctccaccg gcccgatgcg cggagtctcg
     3901 tagcctccca ggcactgcag cagcctgttg cgcagcggat gcatgccggc aagctcctgc
     3961 ggtatgcggc cctcgtccac cagctgctgc acaacggtgt ggtcgcgtga gcgggcgatg
     4021 acctccccgc cgcgcaggag gtagaagcgg cagtcgccga cgtgcgtcca gtgaacgcgg
     4081 ccctcttgca ctacgcaggc ggcgattacg gtgcgcggat ggtcgatcag gtcgcgtttt
     4141 tccgcctcgc gcacgatggc ggcgtgaccg gccgcgagcg agcgcacgag gaagccggcc
     4201 gggtcggcga cgagcggccg cgcctcggct tcgtacgacg cgcccacgac gtcgaccacc
     4261 acctgcgcgg cgagctcgcc gtgcgcatgg ccgcccaggc cgtcggcgac cgccatcgcg
     4321 accgacgcgg acgagctcca gtgggcgacg cggtcctcgt tcttctcgcg cgcgccgatc
     4381 cggctttcct ggacgatgat cggctgtttc attggcggtg gtggtttgcg gcgcgagcgt
     4441 tggcttgcgc ggccatctac cctatgatag ccctctccct tgtcgcttcc cgttcaaagc
     4501 atgaccggct acgccgcggc ggccgccggt ttcgcccgcg gaacgctcgg cctggagctg
     4561 cgttccgtga actcgcgctt cctcgacctg caggtccgca tcgccgacga gctgcgcgcc
     4621 cacgagccgc tgctgcgcga attgatagcc gcccgcatct cgcggggcaa ggtcgactgc
     4681 cggctctccc tcgccgagtc gacggggcaa acggcctcgc gattgaacgc cgaagcgata
     4741 agccggctgc gctcgctcgc cgacgccgcg cggggcgctt tcccggaggc cgcaccgctt
     4801 cgcgtggccg acatcctgcg ctggccgggg gtgatcgccg aaatcggcga cgacgagagc
     4861 ctgcgtgcca cggtggcggg gctctgccgg cgcgcgctcg atgagctcgt cgcgacgcgc
     4921 acgcgcgagg gcgaaaagct ggccgccacg gtgctcgagc gggttcacgc catgcacgcg
     4981 cgccttggtg aaatcgcgcc gctcatgccc caggcgatcg cggcgttcca ggagaagctc
     5041 g
//
LOCUS       DASUNT010000675         2479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_350308, whole genome shotgun sequence.
ACCESSION   DASUNT010000675 DASUNT010000000
VERSION     DASUNT010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350308"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFX94_13545"
     CDS             complement(<1..594)
                     /locus_tag="VFX94_13545"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5769253.1"
                     /translation="MKRWLLAVLAALLPLVSQAQSWPQRPVKFVLPLGPGSGADITAR
                     MLAERLSKQWGQPVVVENRPGADGVVAINTLLQARDDHTLLYAPASSFVGHPYTLEKV
                     PYDAKELLPVARVSNTVVALAVPAALKVQSLNELMQLVREEPGKYNFTAVTNVYDIVF
                     NAYFKSNGLNMVKVSYKDTVSALNDLVENRIQLYSAAY"
     gene            complement(613..993)
                     /locus_tag="VFX94_13550"
     CDS             complement(613..993)
                     /locus_tag="VFX94_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5769254.1"
                     /translation="MIDRIDHIVLTTRDREGCIRFYAEVLGMTLEKFAEGRLALKFGN
                     QKINLHEWGKEFTPRAHVAAPGTLDLCFIASMPLEQVIENLKKNRVPLLEGPVAKTGA
                     LGPMRSVYVRDPDLNLVEISVYNK"
     gene            complement(990..2162)
                     /locus_tag="VFX94_13555"
     CDS             complement(990..2162)
                     /locus_tag="VFX94_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX5769255.1"
                     /translation="MSGPLAGLRVLELGSLVAGPFCGKTFADFGAEVVKIEPPGEGDP
                     LRKWRRMRNGTSLWWQVQSRNKKSVTCDLRRPEGQEIVRRLARRAHVVVENFRAGALE
                     KWNLGWDALSRDNPKLVLVRISGYGQTGPYRERPGFAAIAEGMAGFRYITGFPDRPPV
                     RPNLSIGDTIASLHGVIGALMALHSGKGQVVDVALYEAMFNCMESLIADYDGDGYVRE
                     RTGSTLPGIAPSNLYPCKEGYVLIAGNADSLYHRLMTAIGRPDLRDDPALARNDGRAA
                     RMERIDEAIAEWTRSRSMEEVLSGMEKAQVPAGRIYSAADIAADPHFAARGMIAQVIA
                     GDGEPLKVPGVVPKLSATPGAIGSPAPKLGEHTEEVLQGLGYDHGAILELRKNHII"
     gene            complement(2159..>2479)
                     /locus_tag="VFX94_13560"
     CDS             complement(2159..>2479)
                     /locus_tag="VFX94_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl dehydratase"
                     /protein_id="HEX5769256.1"
                     /translation="TLTGLTEEIFAVRDEKQLVPGALVYSFAEGLLLPSMQDTGLAFL
                     NATLDIKGPTFVGDTIHVECEVTEQRRASKGERGLVRFLNRVLTQNNEVVLEYNPLRL
                     LKMR"
BASE COUNT          387 a          817 c          835 g          440 t
ORIGIN      
        1 gtaggcggcc gagtagagct ggatgcggtt ctcgacgaga tcgttgagcg cgctcaccgt
       61 gtccttgtag gataccttca ccatgttcag cccgttgctt ttgaaatacg cgttgaaaac
      121 gatgtcgtac acattggtga ccgcggtgaa gttgtacttg cccggctcct cgcgcacgag
      181 ctgcatgagc tcgttcagcg actgcacctt gagcgcggcg ggtaccgcga gcgccacgac
      241 agtgttggag acgcgcgcga cgggaagcag ctctttggcg tcgtagggca ccttctcgag
      301 cgtgtaggga tggccgacga aggacgaagc cggcgcatac agcagcgtat ggtcgtcgcg
      361 cgcctggagc aacgtgttga tcgccaccac gccatcggcg ccgggccggt tctcgaccac
      421 caccggctgg ccccattgct tggagagccg ctcagcgagc atgcgcgcgg tgatgtcggc
      481 acccgagccc gggccgaggg gcagcacgaa cttcaccggg cgctgcggcc aggactgcgc
      541 ctgcgagacg agcggcagca gcgccgcgag cacggccagt agccagcgtt tcatgtttgg
      601 tttcctcctc ctttatttat tgtagaccga gatctccacg agattcaggt cggggtcgcg
      661 cacgtacacc gagcgcatcg ggcccagcgc ccccgtcttc gccacggggc cctccaggag
      721 gggaacgcga ttctttttca gattctcgat cacctgctcg aggggcatcg aggcgatgaa
      781 gcacaggtcc agcgttcccg gcgcggcgac atgcgcgcgc ggcgtgaact ccttgcccca
      841 ctcgtgcagg ttgatcttct ggttcccgaa cttgagagcg aggcgaccct cggcgaattt
      901 ctcgagcgtc atgccgagca cttcggcgta gaagcggatg cagccttcgc ggtcgcgcgt
      961 ggtgagcacg atgtggtcga tgcggtcgat catatgatgt ggttctttcg tagctcgagt
     1021 atcgcgccgt ggtcgtaacc caggccctgc aagacctcct cggtgtgctc gccgagcttc
     1081 ggcgcaggcg agccgatcgc gcccggcgtg gccgaaagct tcggcacgac tcccggcacc
     1141 ttgagcggct cgccatcgcc ggcgatcacc tgcgcgatca tgccgcgcgc ggcgaagtgc
     1201 gggtcggccg cgatgtcggc cgcgctgtag atgcgccccg caggcacttg cgccttctcc
     1261 atcccggata ggacttcctc catggaacga cttcgcgtcc attcggcgat cgcctcgtcg
     1321 atgcgctcca tccgagcggc gcggccgtcg ttgcgcgcga gcgccgggtc gtcgcgcagg
     1381 tcggggcggc cgatcgccgt catgaggcgg tggtagaggc tgtcggcgtt gccggcgatc
     1441 agcacgtacc cttccttgca gggatacagg ttcgagggcg cgattcccgg cagcgtgctg
     1501 ccggtgcgct cgcgcacgta gccgtcgccg tcgtagtcgg cgatcaggct ctccatgcaa
     1561 ttgaacatcg cctcgtagag cgcgacgtcg acgacctggc cttttccgga atgcagcgcc
     1621 atcagcgcgc cgatcacgcc atgcagcgat gcgatggtgt cgccgatgga gaggttcggc
     1681 cgcaccggcg gccggtcggg gaagccggtg atgtagcgaa accccgccat gccctcggcg
     1741 atcgcggcga agcctggccg ctcgcggtaa gggccggtct gcccgtagcc ggagatgcgc
     1801 accagcacga gcttcgggtt gtcgcgcgaa agcgcgtccc agccgaggtt ccacttctcg
     1861 agcgcgccgg cgcggaagtt ctcgaccacg acgtgcgcgc gccgcgcgag ccggcgcacg
     1921 atctcctggc cctcggggcg gcgcaggtcg caggtgaccg acttcttgtt gcgcgactgc
     1981 acctgccacc agagcgaggt gccgttgcgc atgcggcgcc acttgcgcag cggatcgccc
     2041 tcccccggcg gctcgatctt gaccacctcg gcgccgaagt cggcgaaggt cttgccgcag
     2101 aacggcccgg cgaccaggct gccgagctcg agcacgcgca ggcccgccaa gggtccgctc
     2161 atctcatctt caagagccgc agcgggttgt actcgagcac cacctcgttg ttctgcgtca
     2221 ggactctatt caggaagcga accagcccgc gctcgccctt cgaggcgcgg cgctgctcgg
     2281 tcacctcgca ctcgacgtgg atcgtgtcgc cgacgaaggt cgggcccttg atgtcgagcg
     2341 tggcgttgag gaacgcgagt ccggtgtcct gcatgctcgg caggagcagg ccttcggcga
     2401 aggaatacac gagcgcgccg ggaacgagct gcttctcgtc ccggacggcg aaaatctcct
     2461 cggtcagccc ggtcagcgt
//
LOCUS       DASUNT010000676         5189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_611073, whole genome shotgun sequence.
ACCESSION   DASUNT010000676 DASUNT010000000
VERSION     DASUNT010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611073"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VFX94_13565"
     CDS             complement(<1..834)
                     /locus_tag="VFX94_13565"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5769257.1"
                     /translation="MDLRVTTPGLLGALVVGLAVWILHSFLAALLVACVTALATWPLY
                     RRFAALLQSHVSRGAISVAFTLLVSALVLAPLIFACWALLAKASALLLDIAAADAQGI
                     GVPPWLQGVPLAGAWLAEHWRNELAHPGALWAWVQQRADAAALLGWLQGFGQLMGRQL
                     VIIGFAILVLAFLYQEGESLARELKRALRHLLGERAEGYIDVAARAVRASANSMLVVA
                     LFDGLASGIAYAIAGVPEPLTWAAITGLLALVPFLGYGAVAALALELALTGASTAALV
                     SL"
     gene            complement(1000..1581)
                     /locus_tag="VFX94_13570"
     CDS             complement(1000..1581)
                     /locus_tag="VFX94_13570"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX5769258.1"
                     /translation="MNLRSVFVAVLAAAATAAQGVDVSQSMILVAKPELVDQVFGSSI
                     LVVAPVGGDQHAGFIVNRPTNVTLAELFPEDGPSQKVIDPVYLGGPLNQQAIFALVER
                     EQSPGGQSFEVMPGLYAAVDEETVDRMIRAESDHARFVVGLVVWRPGELRAEIERGAW
                     YVLPPDAQLALRKPSQGLWEELVHLSKLRANSI"
     gene            complement(1612..1896)
                     /locus_tag="VFX94_13575"
     CDS             complement(1612..1896)
                     /locus_tag="VFX94_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769259.1"
                     /translation="MKPSEHALSEARRQHTEAIVAQHVHELFQRLPMLSGFWLQPDLE
                     VTELSVFTWPGYTAGRELYEEVVQSLVDLADERPEAVPLIRGRTFARAVH"
     gene            complement(1910..2407)
                     /locus_tag="VFX94_13580"
     CDS             complement(1910..2407)
                     /locus_tag="VFX94_13580"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX5769260.1"
                     /translation="MRTDPSGARLRFDRFELDEAEVRLTRAGEPVALAPKPFGVLCAL
                     ARRPGMLVTKDALLDEVWGHRFVTESVLKSAISELRAALDDDPKEPRYIETVSRRGYR
                     FIAGVSGASSQSAAAVERHGVADSGHPALTALTALCRCDAALAELIRSVAARLERERL
                     VLNDS"
     gene            2754..3206
                     /locus_tag="VFX94_13585"
     CDS             2754..3206
                     /locus_tag="VFX94_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HEX5769261.1"
                     /translation="MSCKHLVTALLALVFLGACESNPSKEDIGMATGAVLGGVIGHQF
                     GSGSGNTIATIGGAALGAFVGSRIGRSMDRDDQRKTAAALESSPDNRATTWKNPNTGQ
                     NYTVTPTRTYEGASGPCREFKSTTVIDAREEVVHGTACRQPDGSWKAA"
     gene            3229..4341
                     /locus_tag="VFX94_13590"
     CDS             3229..4341
                     /locus_tag="VFX94_13590"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5769262.1"
                     /translation="MDALLRTQAGELVVALPERGIEGACLARPLAELGLKSAGAIRCV
                     RVEAPPAWHWTTQDAAFLARVLGELRSSGHGMALEGVPQELENLLRLACAAPSSGRRE
                     TTRAPGLRENIGLAVFRGAENALAFVALLGSVVLLLPRFFAARARIRRAELIEVLAES
                     SSRALAIVAVTNLLMGAILAFVGAVQLQTFGAGIYVANLVGIASVRELTPILTAIVLA
                     GRTGASFAARIATMQGNEEIDALSVLGVSPVEFLVVPRVVALALLMPLLYVYGCAFAL
                     LGGAAVAMPVVDVSAAAYAAQTQHAVAGAHFAIGALKALVFGSLVALIGCHYGLAAPR
                     SAAGVGTATTSAVVSSILAIIALDAVFALCASALKI"
     gene            4338..5084
                     /locus_tag="VFX94_13595"
     CDS             4338..5084
                     /locus_tag="VFX94_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5769263.1"
                     /translation="MNAQRTPRIRLEDVALGYAADTVQSGVSAAIAQGEVFAVVGDSG
                     SGKSTLLKALIGLMPVRSGRIEMDGMALAERLREGPPPFGVLFQNGALWTSMTVLENV
                     MLPMELHRYAHPAARAGLARFKLALVGLAGNEERYPASLSGGMRKRAALARALALDPG
                     VLFLDEPSAGLDPLASRRLDELILGLRDGLGITVVLVTHEVESLYRVADRMIFLDGEA
                     HRPLALGAPAELARSAQSAKVREFLSRHVQ"
BASE COUNT          832 a         1773 c         1795 g          789 t
ORIGIN      
        1 gagcgagacc agcgccgcgg tcgaggcgcc ggtcagcgcg agctccagcg cgagcgcggc
       61 cacggcgcca tagccgagga acggcacgag cgcaagcaat ccggtgattg cggcccaggt
      121 gagcggctca ggcacgccgg cgatggcgta ggcgatgccg ctcgccaaac catcgaagag
      181 cgctacgacc agcatgctgt tcgccgacgc acgcaccgcc cgcgccgcga cgtcgatata
      241 gccctcggcg cgttcgccga ggaggtgacg aagcgcgcgc ttgagctcgc gcgccagcga
      301 ctcgccttcc tggtagagaa aagcgagcac caggatggcg aacccgatga tcacgagctg
      361 ccggcccatg agttgcccga agccctgaag ccacccgagc aacgccgccg cgtcggcgcg
      421 ttgttgcacc catgcccaga gcgctccggg atgcgcgagc tcgttcctcc aatgctcggc
      481 aagccaggcg cccgcgagcg gcacgccctg gagccacggc ggaacgccga ttccctgcgc
      541 gtcggcggcg gcgatgtcaa gcagcaacgc gctcgccttg gcgagcagcg cccagcaggc
      601 aaagatcagc ggcgcgagca ccagtgccga caccagcaac gtgaacgcga cagaaatcgc
      661 gccgcgcgag acgtgcgact gcaatagcgc cgcgaatcgc cggtaaagcg gccaggtggc
      721 gagcgcagtc acgcaggcga caagcagcgc cgcaaggaag ctgtgcagga tccagaccgc
      781 cagcccgacg acgagcgcgc ccagcagccc tggcgtggtc acgcggaggt ccatgttccg
      841 ggcatgatga agcgttcgcc gtgcaaagtc gtcagcgaac ggtgaagttt tggtcggggt
      901 tgaccaagcg gtcattcccg cgcatgcggg aaactatgtc attcaaaaaa gaaaacgggg
      961 cccccgcgtt cgcgggggcg acgtgttagt accagccggc tagatgctgt tcgcgcgcag
     1021 cttcgagagg tgcacgagct cttcccacag gccctggctc ggcttgcgca gcgcgagttg
     1081 cgcatcgggc gggagcacgt accatgcccc ccgctcgatt tcggcgcgca gctcgcccgg
     1141 ccgccagacg accaggccga cgacgaagcg agcgtggtcc gattcggcgc ggatcatgcg
     1201 atcgaccgtc tcctcgtcga ccgcggcata aaggcccggc atgacctcga aggactggcc
     1261 accggggctc tgctcgcgct cgacgagcgc gaagattgcc tgctggttga gcggcccgcc
     1321 gaggtagacg gggtcgatta ccttctgcga aggaccgtcc tccgggaaga gctcggcgag
     1381 ggtcacgttg gtcgggcggt tgacgatgaa cccggcgtgc tggtcgccgc ctaccggcgc
     1441 gactacgagg atgcttgagc cgaacacctg gtccacgagc tccggcttcg ccacaagaat
     1501 catcgactgc gaaacgtcca caccctgtgc cgcggttgcc gctgcggcga gcactgcaac
     1561 gaaaacgctc ctcaggttca tcgtcgcctc ctggttaggg aaaccggctg ctcaatgcac
     1621 cgcgcgagcg aacgtgcgcc cgcggatgag cgggaccgcc tcgggacgct cgtcggccag
     1681 atcgacgagc gactgcacca cttcctcgta cagctcccgc cctgcggtgt agcccggcca
     1741 ggtgaacacc gagagctcgg tcacctcgag gtcgggctgc agccagaagc ccgacagcat
     1801 cggcaggcgc tggaacagtt cgtgtacgtg ctgggcgacg atcgcctcgg tgtgctggcg
     1861 acgcgcttcg cttagtgcgt gctcggaggg tttcatgcga gcctcccgtc taggagtcgt
     1921 tgaggacaag ccgctcgcgc tcgagtctcg cggcgaccga gcggatgagc tcggcgaggg
     1981 cggcatcgca gcggcacaat gccgtgagcg ctgtcagggc aggatgtccg gagtcggcga
     2041 caccatgtcg ttcgacagcc gccgcgctct gcgaggaggc gccgctcacg ccggcgatga
     2101 agcggtagcc gcgccgcgac accgtctcga tgtagcgcgg ctctttgggg tcgtcgtcga
     2161 gggcggcgcg cagctcgctg attgccgact tgagtaccga ctcggtgacg aagcgatggc
     2221 cccagacctc gtcgagaagc gcgtccttcg tgaccagcat gccgggcctg cgtgccagtg
     2281 cgcaaagaac tccgaacggc ttcggggcga gcgccacggg ctcaccggcg cgcgtcagcc
     2341 gtacctcggc ctcgtccagc tcgaaacggt cgaagcgcag gcgcgcgcca gacggatcag
     2401 ttctcatgct attgctcctt ccactgcttc cgggatggga agaaagagcc tcgccacgcc
     2461 tgggacttcc ctggggggag gagggagggg tagggaagtg ccaggactgc cgtggcgagg
     2521 tcgtaaggtt cacgatgcaa agcctaggcg catgcgattc atcctgccat tgcgccgagg
     2581 tacaccggtg ttgtgcgcag cgactgaaac gctgccgcac gggatgacgg cctaagactt
     2641 tggtataggt ccgtgctgcc aatcggcaag cgcctcgcgc caaccaaagg ccgacggaag
     2701 acagacgctt cgctgacgac tggcgcgcgg cggacgggta cgctgctcta gtcatgtcct
     2761 gcaaacacct cgtgaccgct ttgctcgcgc tagtcttcct gggtgcgtgc gagtccaacc
     2821 cctcgaagga agatatcggc atggcgaccg gggccgtcct cggcggcgtg atcgggcacc
     2881 agttcggaag cggcagcgga aacacgattg ccaccatcgg cggcgcggcg ctcggcgcgt
     2941 tcgtaggcag ccgcatcggt cgcagcatgg accgcgacga tcagcgcaag accgcggcgg
     3001 cgctggagag ctcgccggac aatcgggcca ccacctggaa aaaccccaat accgggcaaa
     3061 actacaccgt caccccgact cgcacctatg agggcgcctc gggtccgtgc cgagagttca
     3121 agtcgaccac tgtgatcgac gctcgcgagg aggtcgtgca cggcacggcg tgcaggcagc
     3181 ccgacggcag ctggaaagcc gcgtgaacgg gcgcgccggc gaatcatcgt ggatgccttg
     3241 ctcaggacgc aggcgggcga gctcgtcgta gcgttgcccg agcgcggcat cgaaggcgcg
     3301 tgcctcgcgc ggccgctggc cgagctcggt ctgaaatccg cgggcgccat ccgttgcgtt
     3361 cgcgtggaag caccgcccgc atggcactgg acgacgcagg acgcggcatt tctcgcccgg
     3421 gtgctcggcg agctgcggtc atccgggcat ggcatggcgc tcgaaggcgt gccgcaggag
     3481 ctggagaacc tgctccggct tgcctgcgcc gcgccgtctt ccggccgccg ggaaacgacg
     3541 cgcgcgcccg gcctgcggga gaacatcggt cttgccgtct tccggggcgc ggagaacgcg
     3601 ctcgcgttcg tcgccctgct gggatcggtc gtgctcctgc tgccgcggtt tttcgccgca
     3661 cgggcgagga tccgccgcgc cgagctgatc gaggtgctgg ccgagtcgag cagccgcgcg
     3721 ctcgcgatcg tcgccgtgac gaacttgctg atgggcgcca ttctcgcgtt cgtcggcgcc
     3781 gtgcagttgc agaccttcgg cgccggcatc tacgtcgcca acctggtcgg catcgcctcg
     3841 gtgcgggagc tcacgcccat cctcacggcg atcgtcctcg ccgggcgcac cggcgcgagc
     3901 ttcgccgcac gcattgccac catgcagggc aacgaggaga tcgatgcgct cagcgtcctc
     3961 ggcgtgtcgc cggtcgagtt cctcgtcgtg ccgcgcgtgg tcgcccttgc cctgctgatg
     4021 ccgttgctct acgtctacgg ctgcgcgttc gcgctgctcg gcggagctgc cgtcgccatg
     4081 ccggtggtcg atgtgtcggc cgcggcgtac gcagcgcaga cgcagcatgc cgtcgccggc
     4141 gcgcacttcg cgatcggcgc gctgaaggcg ctcgtcttcg gcagcctcgt cgccctcatc
     4201 ggttgccact acgggctggc cgccccgcgc agcgcggccg gcgtcgggac ggcgacgacc
     4261 tccgcggtgg tgagctcgat cctcgccatc atcgcgctgg atgccgtgtt cgcgctctgc
     4321 gccagcgcgc tcaagatatg aacgcacagc gcaccccccg cattcgcctg gaggacgtgg
     4381 cgctcggcta cgccgccgac acggtgcaga gcggcgtgag cgcggcgatc gcccagggcg
     4441 aagtcttcgc cgtcgtcggc gacagcggct cgggcaagag cacgctcctc aaggcgctga
     4501 tcgggctgat gcccgtgcgc agcggccgca tcgagatgga cgggatggcg ctcgccgagc
     4561 gcctgcgcga gggccctccg ccattcggtg tgctgttcca gaacggtgcg ctgtggacct
     4621 ccatgaccgt gctcgagaac gtgatgctgc cgatggagct ccatcgctac gcgcacccgg
     4681 cggcgcgcgc cgggctcgcc cgcttcaagc tcgccctcgt cgggctcgcg ggcaacgagg
     4741 agcgatatcc cgcgagcctg agcggcggca tgcgcaagcg cgcggcgctg gcgcgcgcgc
     4801 tcgcgctcga cccgggcgtg ctctttctcg acgagccctc cgccgggctg gacccgctcg
     4861 cctcgcgccg gctcgacgag ctcatcctcg gcttgcgcga tggcctggga atcacggtgg
     4921 tgctcgtcac ccacgaggtc gaaagcctct accgcgtcgc cgaccgcatg atcttcctgg
     4981 acggcgaggc ccaccggccg ctcgcgctcg gggccccggc cgagctcgcg cgctcggcgc
     5041 agagcgcgaa ggtgcgcgaa ttcctctcgc ggcacgtcca atgaagaagc caagcgcgac
     5101 cctcatcggc acgttcgtcc tcgcggcgct cgcgcttgtg gtggcgggca ttctcttttt
     5161 cggccgcggc gcgctctccg agaagcgct
//
LOCUS       DASUNT010000677         2739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_874678, whole genome shotgun sequence.
ACCESSION   DASUNT010000677 DASUNT010000000
VERSION     DASUNT010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874678"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1758
                     /gene="treZ"
                     /locus_tag="VFX94_13600"
     CDS             46..1758
                     /gene="treZ"
                     /locus_tag="VFX94_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX5769264.1"
                     /translation="MNVPHRIAAQHAMPFGAEVLAQGGVRFRLWAPAATKIELLIAGR
                     MIALEPSGEGWFEHVDAQAGPGTLYRYRVDGELLVPDPASRFQPRDVDGPSEVIAPRS
                     YRWSDHAWNGRAWNEAVIYELHVGTFTAEGTYAGAAKKLSTLKDLGITALELMPLADF
                     AGRRNWGYDGVLPYAPDSAYGRPEDLKALVDAAHAAGLMVFLDVVYNHFGPKGNYLPR
                     YAPQFFTERYRTPWGAAIDYANPTVREFFLHNALYWLEEYRFDGLRFDAVHAIIDPSP
                     CHILEEICVRAPAGKHLVLENDANQAGLIGPGRYTAQWNDDSHHAYHVLATGESDGYY
                     AAYADSPAKHLARSLAEGFAYQGEVSPFTREPRGEPSAHLPPACFVDFLQNHDQIGNR
                     AFGERLASLSRERELKALSAIHLLAPSPPLIFMGEEWGSRQPFLFFCDFDGELGEAVR
                     KGRREEFSRFAGFSDAVPDPLAESTFRQCVLDWGKRDPVWLSHYKKLLQLRARAIAPR
                     TFGPGRYHMFCEYAFVVQWDGLMLLANCANKAISVTGTPDDKPLWSNGRPGDPWSVNW
                     WLTK"
     gene            1743..>2739
                     /gene="malQ"
                     /locus_tag="VFX94_13605"
     CDS             1743..>2739
                     /gene="malQ"
                     /locus_tag="VFX94_13605"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX5769265.1"
                     /translation="MADEVAPSYLDVWGNERPIEPEVRRVLLEAMGPRRQARKVRLSL
                     GRCYQPPVLQERRLWGFMVQLYGARSGRNWGIGDFTDLRALIETAAALGAAVVGVNPL
                     HATQRSPYSPSSRHALNILYIDVEALPGYAPSTRLRRRLQALRDSELVDYEGVRRAKI
                     PVLEDLFAKKKPQVRLPTKALHDFAVFEALREEFGDGWQSWPEAFHHPDSKAVKAFAR
                     KNETRVSFHSWLQSVAREQLQGAARYAHELGMPLGLYVDLALGADRGGAEVWSDQDAY
                     ALGASIGAPPDEFNPLGQDWGLPPYSPRALRAKAYRPFIELLRANMPAGGALRMDH"
BASE COUNT          441 a          933 c          914 g          451 t
ORIGIN      
        1 ggcgccggaa accctgagcc gtgaaaccgg tcacacctcg gtatcgtgaa cgtcccgcat
       61 cgcattgccg cgcagcacgc catgccgttc ggcgccgaag tcctcgcgca gggcggcgtg
      121 cgattccggc tgtgggcgcc cgcggccacg aagattgaac tgctgattgc gggccgcatg
      181 atcgcgctcg agccgagcgg cgaaggctgg ttcgagcacg tcgatgcgca ggccggcccg
      241 ggcacgctgt accgctatcg cgtggatggc gagctcctgg tgccagaccc ggcgtcgcgc
      301 ttccagccgc gcgatgtgga cggtccgagc gaagtgatcg ctccgcgctc ctaccgctgg
      361 agcgaccacg cctggaacgg cagggcgtgg aacgaggcgg tgatctacga gctccatgtg
      421 ggcacgttca ctgccgaggg cacctatgcg ggcgcggcga aaaaattaag cacgctgaaa
      481 gacctcggca tcacggcgct cgagctcatg ccgctggcgg atttcgcggg ccggcgcaac
      541 tggggctacg acggcgtgct gccgtacgcg cccgattcgg cctacggccg gcccgaggac
      601 ctgaaggccc tcgtggacgc cgcgcatgcc gcaggcctga tggtgttcct cgacgtcgtg
      661 tacaaccact tcggccccaa gggcaactac ctgccgcgct acgcgccgca gtttttcacc
      721 gagcgctacc gcacgccctg gggagccgcg atcgactacg ccaacccgac ggtcagggaa
      781 ttcttcctcc ataacgcgct gtactggctg gaggaatacc gcttcgacgg ccttcgcttc
      841 gacgcggtgc acgccatcat cgacccttcg ccgtgccaca tcctggaaga aatctgcgtc
      901 cgcgcgcccg ccgggaagca cctggtgctc gagaacgacg ccaaccaggc gggcttgatc
      961 ggccccggcc gctataccgc gcagtggaat gacgactcgc accacgccta tcacgtgctc
     1021 gcgaccggcg agtccgacgg ctactacgct gcctatgccg attctccggc gaagcacctg
     1081 gcgcgctcgc tcgccgaagg ctttgcctac cagggggaag tttcgccgtt cacccgcgag
     1141 ccgcgcggcg agccgagcgc gcacctgccg cctgcgtgct tcgtggactt ccttcagaac
     1201 cacgaccaga tcggcaaccg ggcattcggc gaaaggctcg cttcgctttc cagagaaaga
     1261 gagctcaagg cgctgagcgc gatccacctg ctcgccccgt cgccaccgct cattttcatg
     1321 ggcgaggaat ggggctcgcg ccagccgttc ctcttcttct gcgattttga cggcgagctc
     1381 ggcgaagcgg tgcgcaaggg ccggcgcgag gagttcagcc gcttcgccgg cttctcggac
     1441 gcggtcccgg atccgctcgc agagagcact ttccgccaat gcgtgctcga ctggggcaag
     1501 cgcgacccgg tctggctctc gcattacaag aagctcctcc agctgcgcgc gcgtgccatc
     1561 gcgccgcgca cgttcgggcc cggcaggtat cacatgttct gcgagtacgc gttcgtggtg
     1621 cagtgggatg gcctcatgct gctcgcgaat tgcgccaaca aggcgatctc ggtgacgggt
     1681 acgccagacg acaagccgct gtggtcgaac ggcaggccgg gcgatccctg gtcggtgaac
     1741 tggtggctga cgaagtagct ccgagctatc tcgacgtctg gggcaacgag aggccgatcg
     1801 agcccgaggt acgccgcgtc ctcctcgagg cgatggggcc gaggcgccag gcgcgcaagg
     1861 tgcgcctttc gctcgggcgc tgctaccagc cccccgtcct ccaggagcgc cggctctggg
     1921 gcttcatggt gcagctctac ggggcacgct ccgggcgcaa ctggggcatc ggcgacttca
     1981 ccgacctgcg cgcgctgatc gagaccgccg ccgcgctcgg cgcagcggtg gtcggcgtga
     2041 atccgctcca tgccacgcag cgcagcccgt acagtccctc cagccgccac gcactcaaca
     2101 tcctgtatat cgatgtcgaa gcgctgcccg gctacgcgcc ctccactagg ctgcgccgcc
     2161 ggctgcaagc gctgcgcgat tccgagctgg tggattacga gggcgtgcgc cgggccaaga
     2221 ttcccgtact ggaagatctg ttcgcgaaga agaaaccgca ggtccgcctt ccgacgaaag
     2281 cgctgcatga cttcgcggtg ttcgaagcgc tgcgcgagga atttggggac ggctggcaat
     2341 cctggcccga ggcttttcac cacccggatt caaaagcggt caaggcgttt gcacgcaaaa
     2401 atgagacgcg cgtttccttt cattcgtggc tgcagagcgt ggcgcgcgag cagctccagg
     2461 gggcggcgcg ctacgcgcac gagctcggca tgccgctcgg gctgtatgtg gaccttgcgc
     2521 tgggcgccga tcgcggcgga gcggaggtgt ggtcggacca ggacgcgtac gcgctcggcg
     2581 cttcgatcgg agcgccgccc gacgagttca acccgctcgg gcaggactgg ggcctgccgc
     2641 cgtattcgcc gcgcgcgctg cgcgcgaagg cctatcggcc gttcatcgag ctcttgcgcg
     2701 cgaacatgcc ggccggcggc gcgctgcgta tggaccatg
//
LOCUS       DASUNT010000678         4743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_85851, whole genome shotgun sequence.
ACCESSION   DASUNT010000678 DASUNT010000000
VERSION     DASUNT010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85851"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /gene="cyaY"
                     /locus_tag="VFX94_13610"
     CDS             <1..312
                     /gene="cyaY"
                     /locus_tag="VFX94_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEX5769266.1"
                     /translation="SEFEALAGGALRRIETALEASGIDADFELKEGGILEIEFEDGAK
                     MIVNRHGAAREIWVAARSGGFHFRWDGAAWRDTRSGEELFAALSELLSRQLGRRVDLR
                     Q"
     gene            complement(283..2607)
                     /locus_tag="VFX94_13615"
     CDS             complement(283..2607)
                     /locus_tag="VFX94_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEX5769267.1"
                     /translation="MWIRYVSLPLAILAGLALAAVLAAGLVVVLAYPQLPSLEALGSQ
                     PKIPLRIYTADGVLIGEFGEERRAVISIAEVPQPLKNAVIAAEDERFYEHPGIDYIGV
                     LRAAWANLVAGGRRQGASTITMQVARNFFLSSEKTLTRKLYEALLAFKIEHSFSKEQI
                     LELYVNQIYLGQRAYGFAAASQTYFGKALGQLTLAETAMLAGLPKAPSLYNPVANPQR
                     AKQRQQYVLRRMNELGYIDATQYEDAVKTPLRTRGRELAEYSVHAEFAAEMVRQALVE
                     HYPDEVYTRGFRVYTTLRKADQEAAYHALRQGVLEYDRRAGYRGPEAYVELPANPTEE
                     DVEDALDGHPDNDDLLAAVVLAYDGKQVQAGLRSGERIAIAGPGLQFVARALDPKAAT
                     QRRIRKGAIIRVQRDAKGWHIVQLPEVEAAFISLDPQDGAIRALVGGFDFSRNKFNHV
                     TQAWRQPGSSFKPFIYSAALEKGFTAATVVADEPVVLEAEQTGSQRWEPKNYDGKFEG
                     PMRLRTALAKSKNMVSIRVLDAIGPRYAQDYVTRFGFEAERHPAYLTMALGAGSVTAW
                     QMARAYSVFANGGFLIQPYFIHKIVDDRGNALALAEPRRAGDEAMRVLDPRNAFIMDH
                     MMQDVTRYGTAARAARLGRSDLAGKTGTTNEFVDAWFAGYQPGLVAVAWVGFDQPKTL
                     GKNQTGGVVALPIWLGYMEHVLREFPEMSRVVPEGLVSVPVGPLAGESTKLVPEFFYR
                     EAVPPPDVLQPLPPPPLPAQPAQPPSLPQINPPA"
     gene            2794..3870
                     /locus_tag="VFX94_13620"
     CDS             2794..3870
                     /locus_tag="VFX94_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEX5769268.1"
                     /translation="MKIDLFTAKAPPLFGLDISSSSVKMLELVDGGKSGYRVDRYAIE
                     PLPRDCVMDGNINNLEAVTESVKRAHARMGTRTRHVAMAVPSGAVISKKIIVPAALRE
                     EELEVQVESEANQYIPFALEEVNLDFQILGPSPSNAEEQEVLIAATRKEKVEDRVAVA
                     DSAGLKALVMDVESFAQQTALSLVVQALPGAGRDQNVAVVDVGANVMNVAVLRNDQSI
                     YTREQAFGGNQLTQDIVSRYGMTAEEAESAKRSGGLPDDFDTEVLKPFMENLSMEVQR
                     ALQFFFTSTQYHAVDHILLAGGSAVIPGLDEVVHTRTQVPTSVANPFALMQASPKIQL
                     KRLMSDAPSLIVACGLAMRRFDPQ"
     gene            3867..4475
                     /locus_tag="VFX94_13625"
     CDS             3867..4475
                     /locus_tag="VFX94_13625"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX5769269.1"
                     /translation="MNPRINLLPHRADRRKRARQHFITVAFGTFLVGAALAGLVYQFY
                     AQQIEVQNDRNTFLANEIKKLDKDIADINELRNQIQALLARKQIIETLQADRAQTVHL
                     LEQMVRQMPEGVFLRSMKQTGSKVHIAGFAQSNARVSTLMRNIEASPWLEQPTLVEVK
                     SASVDKRRVSEFNLYLNQKRAEAKDAAKDGAKDAAKAAAKKG"
     gene            4479..>4743
                     /locus_tag="VFX94_13630"
     CDS             4479..>4743
                     /locus_tag="VFX94_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769270.1"
                     /translation="MAINLNAFVEEFKRTNWRDAGTWGAAPKILVLIAILIGVPVAGF
                     FAFNQGQIEELEAGQLKEVKLKEEYVGKKKQAINLDLHRPQLRE"
BASE COUNT          860 a         1526 c         1587 g          770 t
ORIGIN      
        1 agcgaattcg aagcgttggc gggcggcgcg ctgcgccgga tcgagaccgc cctcgaggcg
       61 agcgggatcg acgccgactt cgagctgaag gaaggcggca tcctcgagat cgagttcgaa
      121 gacggcgcga agatgatcgt caaccggcac ggcgcggcgc gcgagatctg ggtcgccgcg
      181 cgcagcggcg gatttcactt ccgctgggac ggagctgcat ggcgcgacac acgcagcggc
      241 gaggagctgt ttgccgcctt gtcggagctg ctttcgcggc agctaggcag gcgggttgat
      301 ctgcggcaat gacggcggct gcgccggctg cgccggcagc ggcggcggcg gcagcggctg
      361 cagcacatcg ggcggcggca cggcctcgcg atagaaaaac tccggcacga gcttggtcga
      421 ctccccggcg agcgggccga ccggcacgga caccagcccc tcgggcacga ctcgcgacat
      481 ctcgggaaat tcgcgcagca cgtgctccat gtagcccagc cagatcggca gcgcgaccac
      541 gccgcctgtc tggttcttgc cgagagtctt cggctggtcg aatccgaccc acgcgacggc
      601 aaccaggccg ggctggtagc cggcaaacca ggcatcgacg aactcgttgg tggtgccggt
      661 cttgccggcg aggtcggagc ggccgaggcg tgccgcacgc gccgccgtgc cgtagcgggt
      721 cacgtcctgc atcatgtggt ccatgatgaa ggcgttgcgg gggtcgagca cccgcatcgc
      781 ctcgtcgccc gcgcgccgcg gctcggccag ggcgagcgcg ttgccgcgat cgtccacgat
      841 cttgtggatg aagtagggct ggatcaggaa gccgccgttg gcaaacaccg aataggcgcg
      901 cgccatctgc caggcggtga ccgagcctgc gccgagcgcc atggtcagat aggcggggtg
      961 gcgctcggcc tcgaaaccaa agcgcgtgac gtaatcctgc gcgtacctgg gcccgatcgc
     1021 gtccaggacg cgaatcgaaa ccatgttctt tgacttcgcg agggcggtcc gcagccgcat
     1081 cgggccctcg aacttgccgt cgtagttctt cggctcccaa cgctggctgc cggtctgctc
     1141 ggcttccagg acaaccggct cgtcggcaac tacggtggcg gcggtgaagc ccttctcgag
     1201 cgccgcggag taaatgaacg gcttgaacga ggatccgggc tggcgccagg cctgggtgac
     1261 gtggttgaac ttgttacggc tgaaatcgaa gccgcccacc agggcgcgga tcgccccatc
     1321 ctgagggtcg agcgatatga aggcagcctc cacctcgggc agctggacga tgtgccagcc
     1381 tttcgcgtcg cgctggaccc ggatgatggc gcccttgcga atgcgccgct gcgttgccgc
     1441 tttcgggtcg agcgcgcggg cgacgaactg cagccccggc cccgcgatag cgatgcgctc
     1501 gccgctgcgc agaccagcct gtacctgctt gccgtcgtag gcgagcacca cggcggcgag
     1561 gaggtcgtca ttgtccgggt ggccgtcgag ggcgtcctcg acgtcctcct cggtcgggtt
     1621 tgcgggcagt tcgacatacg cttccggacc gcgatagccg gcgcgccggt cgtattccag
     1681 cacgccctgg cgcaaggcgt ggtaggcggc ttcctggtcc gccttgcgca gcgtggtata
     1741 gacacgaaag cctcgcgtgt agacctcgtc ggggtagtgc tctacgagcg cctgccgcac
     1801 catctcagcc gcgaattcgg cgtgcacgga gtactcggcg agctcccggc cgcgggtccg
     1861 cagcggggtc ttcaccgcat cttcatattg cgttgcatca atgtagccaa gctcgttcat
     1921 gcggcgcagc acatattgct ggcgctgctt ggcgcgctgt ggatttgcga ccgggttgta
     1981 gagcgacggc gccttaggca ggcccgcgag catcgcggtt tcggccaggg taagctgacc
     2041 gagcgccttg ccgaaatagg tttgcgacgc tgcggcgaag ccgtacgccc gctgccccag
     2101 atagatctgg ttgacgtaga gctcgaggat ctgctccttc gagaagctgt gctcgatctt
     2161 gaaggcgagt aaagcttcgt aaagcttgcg agtcagcgtc ttttccgaag agaggaaaaa
     2221 gttgcgcgcc acctgcattg tgatggtgct cgcgccctgg cgccggcccc ccgcaacgag
     2281 gttggcccaa gccgcgcgca gcacgccgat gtagtcgatg ccggggtgct cgtagaagcg
     2341 ctcgtcctcc gcggcgatca cggcgttctt gagcggctgc ggaacctcgg cgatcgagat
     2401 gaccgcgcgt cgctcctcgc cgaattcgcc gatcagcacg ccgtcggccg tatagatccg
     2461 taaaggaatc ttcggctggg aaccaagcgc ttcgagcgag ggcagctgcg ggtaggcgag
     2521 caccaccacc aggccggctg ccagcacggc ggcgagcgcc aagccggcca ggatggcgag
     2581 cggcagcgaa acgtaccgga tccacatgtt cttcggactt agagcgggcg ggcgcaggcg
     2641 ttgttgcaag cggtgcgggg acggccggat catacccctt acccctgtgg aaaattgagt
     2701 agacagccgg ggggcttgtt gctagcattt cgagccgttt ctgcgaatcg cacctaagat
     2761 gcggagctta aagggaaaat accggggaga ataatgaaaa tcgacctgtt cacggcgaag
     2821 gcgccaccgc tgttcgggct ggacatcagc tcttcgtcgg tgaagatgct cgagctcgtc
     2881 gacggcggaa agagcggcta tcgcgtcgat cgctacgcca tcgagcccct gccgcgcgac
     2941 tgcgtgatgg acggcaacat caacaacctc gaggctgtta ccgagtcggt caagcgcgcg
     3001 cacgcccgca tgggcacgcg aacccggcat gtcgcaatgg ccgtgccgag cggcgcggtc
     3061 atcagcaaga agatcatcgt gcccgcggcc ctgcgcgaag aagaactcga ggtgcaggtc
     3121 gaaagcgagg cgaatcagta catcccgttc gcgctcgaag aagtgaacct cgacttccag
     3181 atcctcggtc catcgcccag caatgccgag gagcaggagg tcctgatcgc tgccacgcgg
     3241 aaggaaaaag tcgaagaccg cgtcgcggtg gccgattccg ccgggcttaa ggcgcttgtc
     3301 atggacgtcg agtcgttcgc ccagcagacc gcgctttcgc tcgtagtgca ggcccttccc
     3361 ggcgcgggca gggatcagaa cgtcgcggtc gtggacgtcg gcgccaacgt catgaacgtg
     3421 gcggtgctgc gcaacgatca gtccatctat acccgcgagc aggcgttcgg cggcaaccag
     3481 ctcacgcagg acatcgtcag ccgctacggc atgacggcgg aggaggcgga gagcgccaag
     3541 cgctccggcg gcctgcccga cgacttcgac accgaggtgc tcaagccctt catggagaac
     3601 ctttcgatgg aggtgcagcg tgccctgcag ttcttcttta cctctacgca gtaccacgcg
     3661 gtcgaccata tcctgctcgc cggcggctcg gcggtgatcc ccggcctcga cgaggtcgtg
     3721 cacacccgca cccaggtgcc gacgagcgtc gccaacccgt tcgcgctgat gcaggcttcg
     3781 ccgaagatcc agctgaagcg cctgatgtcc gatgcgcctt cgctcatcgt cgcctgcggc
     3841 ctggcgatga ggaggttcga tccccaatga acccgcgcat caacctgctg ccccaccgcg
     3901 ccgaccggcg caagcgtgcg cggcagcact tcatcaccgt cgcgttcggc accttcctcg
     3961 tcggtgccgc ccttgccggc ctcgtgtacc agttctatgc ccagcagatc gaggtccaga
     4021 acgaccgcaa caccttcctc gccaacgaaa tcaagaagct cgacaaggac atcgccgaca
     4081 tcaacgagct gcgcaaccag atccaggcgc tgctcgcgcg caagcagatc atcgagacgc
     4141 tgcaggccga tcgcgcccag acggtgcacc tgctcgagca gatggtgcgc cagatgcccg
     4201 agggtgtttt cctgcgctcg atgaagcaga cggggtccaa agtgcacatc gccggcttcg
     4261 cgcagtccaa cgcgcgtgtc tccacgctga tgcgcaacat cgaggcctcg ccctggctcg
     4321 agcagcccac gctcgtcgag gtgaagtccg ccagcgtgga caagcgccgg gtatcggaat
     4381 tcaacctgta cctgaaccag aagcgcgcag aggccaagga cgccgccaag gacggcgcca
     4441 aggacgccgc caaggccgcg gcgaagaaag gctaggccat ggcgatcaac ctgaatgcgt
     4501 tcgtcgagga attcaagcgg accaactggc gcgacgcggg cacctggggt gcggccccca
     4561 agatcctggt gctcatcgcg attctgatcg gcgtcccggt cgcgggcttc ttcgcgttca
     4621 accagggcca gatcgaggag ctcgaggccg gccagctgaa ggaagtcaag ctgaaggagg
     4681 agtacgtcgg caagaagaag caggcgatca acctggacct ccaccgcccg cagctgcgcg
     4741 aga
//
LOCUS       DASUNT010000679         2560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_874960, whole genome shotgun sequence.
ACCESSION   DASUNT010000679 DASUNT010000000
VERSION     DASUNT010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874960"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1545)
                     /locus_tag="VFX94_13635"
     CDS             complement(<1..1545)
                     /locus_tag="VFX94_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5769271.1"
                     /translation="MLKEFLNNSYLFGGNAPFIEELYEKYLANPQSISEEWRDYFDRM
                     QDLPGAIDKDVAHAPVVESFVQRAKRGEFTAQRTVAAEPVTPERLQVAALLLVTAYRI
                     AGARWATVDPLKRMPRPNIPELEPAYYDLKEADLDQVVNAGSFVGLERVSLRVLVQAL
                     RDTYCRNIGFEYMFISDRAHRQWIQERIEPVRGAPRVSASEQKRLLQKLTEAEQLERY
                     LHTRYVGQKRFSLEGGESLIPSIDELIQRAGAAGVQEIVIGMAHRGRLNVLVNVLGKM
                     PKDLFLEFEGKQASELPSGDVKYHNGYSSDISTPGGPVHLSLAFNPSHLEIVNPVVEG
                     SVRARQRRRDDKTGDQVLPILVHGDAAFAGQGVVMETLNLSGTRGYGTGGTVHLIVNN
                     QIGFTTSDPRDTRSTIYCTDVAKMVEAPIFHVNGDDPEAVLFVTRLALEFRQTFHKDV
                     VIDIVCFRKLGHNEQDEPAVTQPLMYKKISQHPGTRKLYADKLIAQKIIEEAEPENLI
                     RKYRETL"
     gene            complement(1549..>2560)
                     /gene="gltA"
                     /locus_tag="VFX94_13640"
     CDS             complement(1549..>2560)
                     /gene="gltA"
                     /locus_tag="VFX94_13640"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX5769272.1"
                     /translation="ELPNRKQKDEFVDIVTHHTMVHEQLARLYQGFRRDAHPMAVMVG
                     VVGALSAFYHDALDINNPQHRVISAFRLIAKMPTIVAMSYKYSIGQPFVYPKNDLSYT
                     ANFMRMMFGVPTEEYKVNPVLVRALDRIFILHADHEQNASTSTVRLCGSSGANPYACI
                     AAGIASLWGPAHGGANEACLEMLEEIGDASKVPEFVRRVKDKNAGVRLMGFGHRVYKN
                     YDPRAKLMRETCHEVLNELGLHNDRLFELALKLEKVALEDQYFIERKLYPNVDFYSGI
                     VQRALGIPVAMFTCIFSLARTIGWIAQWQEMITDPEYKIGRPRQLYKGATPREVKPID
                     KR"
BASE COUNT          417 a          808 c          850 g          485 t
ORIGIN      
        1 gagcgtctcg cggtacttcc tgatcaggtt ttccggctct gcttcctcaa tgattttctg
       61 tgcgatgagc ttgtccgcgt agagcttgcg cgtgcccggg tgctgcgaga tcttcttgta
      121 catcagcggc tgggtcaccg cgggctcgtc ctgctcgtta tggccgagct tgcggaagca
      181 gacgatgtcg atcaccacgt ccttgtggaa cgtctggcgg aactcgagcg cgaggcgcgt
      241 cacgaaaagc accgcctccg ggtcgtcgcc gttcacgtgg aagatcggcg cctcgaccat
      301 cttcgcgacg tcggtgcagt agatcgtcga gcgcgtgtcg cgcggatcgg aggtggtgaa
      361 gccgatctgg ttgttcacga tcaggtgaac cgtgccgccg gtgccgtatc cgcgcgtgcc
      421 ggaaaggttc agcgtttcca tcaccacgcc ctgccccgcg aacgccgcgt cgccgtgcac
      481 caggatcggc agcacctggt cgccggtctt gtcgtcgcgg cggcgctggc gcgcgcgcac
      541 cgagccctcc accaccggat tgacgatctc cagatgcgag gggttgaagg cgagcgagag
      601 atgcaccggc ccgcccgggg tcgaaatatc ggacgaatag ccgttgtggt acttgacgtc
      661 gccggacggg agctcggagg cctgcttgcc ctcgaactcg aggaacagat ccttcggcat
      721 cttgccgagc acgttgacca gcacgttgag gcgcccgcgg tgcgccatgc cgatgacgat
      781 ctcctgcacg cccgcggcgc cggcgcgctg gatcagctcg tcgatcgacg ggatcaggct
      841 ttccccgccc tcgagcgaga agcgcttctg cccgacgtag cgggtgtgca ggtagcgctc
      901 gagctgctcc gcctcggtga gcttctgcag caggcgcttc tgctcggacg ccgagacccg
      961 gggcgcgccg cgcaccggct cgatgcgctc ctggatccac tggcgatgcg cacggtcgga
     1021 gatgaacatg tactcgaaac cgatgttgcg gcaatacgtg tctcgcaggg cctgcaccaa
     1081 gacacgcaaa gagacccgct ccagccctac aaatgaaccc gcgttcacga cctggtcgag
     1141 gtcggcttcc tttaggtcgt aataagccgg ctcgagctcc gggatgttcg ggcgcggcat
     1201 gcgcttgagc gggtccaccg tcgcccagcg cgcgccggcg atgcggtacg cggtcaccag
     1261 cagcagcgcc gcgacctgca gccgctcggg ggtgaccggc tcggcggcga cggtcctttg
     1321 agcggtgaat tccccgcgct tggcgcgctg cacgaacgat tcgactaccg gcgcgtgcgc
     1381 cacgtccttg tcgatggcac cggggaggtc ctgcatgcgg tcgaagtagt cgcgccactc
     1441 ctcggagatg gactgcggat tggcgaggta tttctcgtac agctcctcga tgaacggcgc
     1501 attgccgccg aagaggtaag agttgttcag gaattccttg agcatgactt atctcttgtc
     1561 gatcggcttc acttcgcgcg gggtggcgcc cttgtagagc tggcgcggcc ggccgatctt
     1621 gtattccggg tcggtgatca tttcctgcca ctgcgcgatc cagccgatcg tgcgcgcgag
     1681 cgagaaaatg caggtgaaca tcgccaccgg gatgccgagc gcacgctgca cgatccccga
     1741 gtagaagtcg acgttgggat agagcttgcg ctcgatgaag tactggtcct cgagcgcgac
     1801 cttctccagc ttgagcgcga gctcgaacag ccggtcgttg tgcaggccga gctcgttcag
     1861 cacctcgtgg caggtctcgc gcatgagctt cgcgcgcggg tcgtagttct tgtagacgcg
     1921 gtggccgaag cccatcaggc gcacgcccgc gttcttgtcc ttcaccctgc ggacgaactc
     1981 ggggaccttg gaggcatcgc cgatttcctc gagcatctcc aggcacgcct cgttcgcccc
     2041 accgtgcgcc gggccccaca gcgaggcgat gccggcggcg atgcaggcgt aggggttggc
     2101 gcccgaggaa ccgcacaggc gcacggtcga ggtggaggcg ttctgctcgt ggtcggcgtg
     2161 caggatgaaa atgcggtcca gcgcgcgcac gagcaccggg ttcaccttgt actcctcggt
     2221 cggcacgccg aacatcatgc gcatgaagtt cgccgtgtac gaaagatcgt tcttcggata
     2281 cacgaacggc tgcccgatcg agtatttgta ggacatcgcg acgatcgtcg gcatcttggc
     2341 gatcaggcga aacgccgaga tgacgcgatg ctgggggtta ttgatgtcga gcgcgtcgtg
     2401 gtagaaggcc gacagcgcac cgaccacgcc caccattacc gccatcgggt gcgcatcgcg
     2461 ccggaagccc tggtacaggc gcgcgagctg ctcgtgcacc atggtgtggt gcgtgacgat
     2521 gtccacgaac tcgtccttct gcttgcggtt cggcagctcg
//
LOCUS       DASUNT010000680         2885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_175371, whole genome shotgun sequence.
ACCESSION   DASUNT010000680 DASUNT010000000
VERSION     DASUNT010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175371"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VFX94_13645"
     CDS             complement(<1..616)
                     /locus_tag="VFX94_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5769273.1"
                     /translation="MDLLLLAAVSGSAVYLTMQFLARKRDRELAEARNDSARFRGLTE
                     LSADWFWETDAEHRIVWLSGGAPVATFLGQTPTYGKRIWEIPGVEVEPRELQAHLEAL
                     EARQSFFDLEIARIDERGARQVHIISGQSRYDAEDHFVGYRGVGRDVTEQRNAERALF
                     RAKERLELALDGGNLAEFHYDAERNELSAGDGWVRFLGHSRSPAI"
     gene            complement(619..2415)
                     /locus_tag="VFX94_13650"
     CDS             complement(619..2415)
                     /locus_tag="VFX94_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX5769274.1"
                     /translation="MEQAVKAAQGKTGGGRLSLAEVLDWLVADRLVDAEPAAQLKKER
                     RYFRGTTHPLVIIAEQKWKKGNTLLTLDVLSEWLAKRVGMEYMHIDPLKIDFAGVTEI
                     MSSAYATRFRILPLAVTAKEAVIATAEPGVRDWEPELARIIKRDIIRVIANPLDIERY
                     QVEFYNLAKSITGASTTISIPGLSNFEQLVELGRTERQLDANDAHVVRVVDWLWQYAF
                     EQRASDIHIEPRRELGIVRFRIDGVLHQVYQIPAPVLAAMTSRIKILARMDVVEKRRP
                     QDGRIKTRSGEQDVELRISTLPTAFGEKIVMRIFDPEVLVRDFSELGFSQEDQARWKE
                     MTDRPNGIVLVTGPTGSGKTTTLYSTLKSLATIEVNVCTIEDPIEMIEPAFNQRQVVS
                     AIELGFAEGVRALMRQDPDIIMVGEIRDLETAEMAVQAALTGHLVLSTLHTNDAPSAI
                     ARLVELGVPAYLLNATLNGVMAQRLVRTLCPHCKQPLELNRAEDQDAWNALVAPWKSR
                     LPTNVYKPVGCLECRMTGYMGRVGVYEVLVFSQAIKALVNQNADLAALREQAFKEGMK
                     PLRVSGAMKIGAGLTTMDEVLNVAPPVRDTAA"
     gene            2483..>2885
                     /locus_tag="VFX94_13655"
     CDS             2483..>2885
                     /locus_tag="VFX94_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEX5769275.1"
                     /translation="MRAKLIHEHGERVFAVIFDTGEDPMKGLERFAAEQNLDASAFTA
                     IGAFSEALLGYFDWEEKDYQHIPVREQTEVLALLGDITLDEAGGRKVHAHVVLGKRDG
                     TACGGHLLAAKVRPTLEVILTESPGHLKRRHD"
BASE COUNT          448 a          983 c          947 g          507 t
ORIGIN      
        1 tgatcgcggg cgagcgcgag tggccgagga agcgcaccca gccgtcgccg gccgagagct
       61 cgttgcgctc ggcgtcgtag tggaattccg cgaggttgcc gccatcgagc gccagctcca
      121 ggcgctcctt cgcgcggaac agcgcccgct cggcgttcct ctgctcggtg acgtcgcggc
      181 ccacgccgcg gtagccgacg aagtggtcct cggcgtcgta gcgcgactgg ccggagatga
      241 tgtgcacctg gcgtgcgccg cgctcgtcga tgcgcgcgat ctcgaggtcg aagaaggatt
      301 gccgcgcctc cagggcttcg aggtgcgcct ggagctcgcg cggttcgacc tcgacgccgg
      361 ggatctccca gatgcgcttg ccgtaggtgg gcgtctggcc caagaaggtg gcgaccggcg
      421 cgccgcccga gagccacacg atgcggtgct cggcgtcggt ttcccagaac cagtccgccg
      481 agagctcggt gaggccccgg aagcgtgccg agtcgttcct cgcctcggcg agctcccggt
      541 cgcgcttgcg cgcgaggaac tgcatcgtga ggtagaccgc gctgcccgac accgccgcga
      601 gcagcaggag gtccatcatc atgcggccgt gtcccgcacg ggcggcgcca cgttgagcac
      661 ctcgtccatc gtggtgaggc cggcgccgat cttcatcgcg cccgagacgc gcagcggctt
      721 catcccttcc ttgaacgcct gctcgcgcag cgcggccagg tcggcattct ggttcaccag
      781 agccttgatc gcctgcgaga agaccagcac ctcgtagacg cccacccggc ccatgtagcc
      841 ggtcatgcgg cactcgaggc agccgaccgg cttgtaaaca ttcgtcggga gcctggattt
      901 ccacggcgcc accagcgcgt tccacgcgtc ctggtcctcg gcgcggttga gctcaagcgg
      961 ctgcttgcag tgcgggcaaa gcgtacgcac caggcgctgc gccatcacgc cgttcagcgt
     1021 ggcgttgagg aggtaggccg gcacgccgag ctcgaccaag cgcgcgatcg cgctcggcgc
     1081 gtcgttggtg tggagcgtgg acagcacgag gtggccggtg agcgccgcct gcaccgccat
     1141 ctcggcggtc tcgaggtcgc ggatctcgcc caccatgatg atgtccgggt cctggcgcat
     1201 cagcgcgcgc accccctcgg cgaagccgag ctcgatcgcg ctcaccacct gcctctggtt
     1261 gaacgccggc tcgatcatct cgatcgggtc ctcgatcgtg cacacgttga cctcgatcgt
     1321 ggcgaggctc ttcagcgtcg aatagagcgt ggtggtcttg cccgagccgg tcggcccggt
     1381 gacgagcacg atgccgttcg gccggtcggt catttccttc cagcgcgcct ggtcctcctg
     1441 cgagaacccg agctcgctga aatcccgcac cagcacctcg gggtcgaaga tgcgcatcac
     1501 gatcttctcg ccgaacgcgg tcggcagcgt cgagatgcgc agctccacgt cctgctcgcc
     1561 gctgcgcgtc ttgatgcgcc cgtcctgcgg gcggcgcttc tcgaccacgt ccatgcgcgc
     1621 caggatcttg atgcggctcg tcatcgccgc gagcaccggc gccgggatct ggtacacctg
     1681 gtgcagcacg ccgtcgatcc tgaagcgcac gatgcccaac tcgcgccgcg gctcgatatg
     1741 gatgtcgctc gcgcgctgct cgaacgcgta ctgccacagc cagtccacca cgcgcaccac
     1801 gtgcgcgtcg ttggcatcca gctgtctctc ggtcctgcca agctcgacca gttgctcgaa
     1861 gtttgacaag cccgggatcg agatcgtcgt gctcgcgcct gtgatcgact tggcgaggtt
     1921 gtagaactcg acctggtagc gctcgatgtc gagcgggttc gcgatcacgc ggatgatgtc
     1981 gcgcttgatg atgcgcgcga gctcgggctc ccagtcgcgc acgcccggct cggcggtggc
     2041 gatgaccgcc tccttcgccg tgacggcgag cggcaggatg cggaagcgcg tcgcgtacgc
     2101 cgaggacatg atttcggtaa cgcccgcgaa gtcgatcttc agcggatcga tgtgcatgta
     2161 ttccatgccg acgcgcttcg ccagccactc gctcagaacg tcaagcgtga gcagcgtgtt
     2221 tccttttttc cacttttgct cggcgatgat gaccagcggg tgggtcgtgc cgcggaagta
     2281 gcgccgctct ttcttgagct gcgccgccgg ctcggcgtcg accagccgat cggccaccag
     2341 ccagtcgagt acctcagcaa gcgagagcct ccccccgccg gttttccctt gtgcggcttt
     2401 aactgcttgt tccatcggcg aaagactata ccgcaaagcg cgcaatcgct tctgcggcac
     2461 gtttgttgcg ctacgtaaaa ccatgcgagc gaagctgatc cacgagcacg gcgagcgcgt
     2521 cttcgccgtg attttcgaca cgggcgagga tccgatgaag ggtctcgagc gctttgcggc
     2581 cgagcagaat ctcgacgcat ccgccttcac cgcgatcggc gcattcagcg aggcgctgct
     2641 cggctacttc gactgggaag aaaaggacta tcagcacatc ccggtgcgcg agcaaaccga
     2701 ggtcctcgct ctgctgggcg atatcacgct cgacgaagcg ggcggcagga aagtgcacgc
     2761 gcacgtggtg ctcggaaagc gcgacggcac tgcgtgcggc ggccatctgc tcgcggccaa
     2821 agtgcgcccg accctcgagg tcatcctgac cgagtcgccg ggccacctga aacgccggca
     2881 cgacc
//
LOCUS       DASUNT010000681         2702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_874996, whole genome shotgun sequence.
ACCESSION   DASUNT010000681 DASUNT010000000
VERSION     DASUNT010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874996"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /gene="yidC"
                     /locus_tag="VFX94_13660"
     CDS             <1..625
                     /gene="yidC"
                     /locus_tag="VFX94_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX5769276.1"
                     /translation="APLFLLLKWLHGLLGNWGWAIVVMTIMLKAAFYPLNAAAARSMG
                     KMKLIAPKMKALQEQYANDKQQLQIKMMEMYKSEKINPLGGCLPILVQIPVFIALYWV
                     LLAAIELRHAPFMLWIKDLSALDPYYVLPILMTGTMILQTRMNPVPPDPVQARVMQIM
                     PFVFSIFFFFFPAGLVLYWLVNNILSIAQQWQIQRMFDRDKPAHAKR"
     gene            612..1934
                     /gene="mnmE"
                     /locus_tag="VFX94_13665"
     CDS             612..1934
                     /gene="mnmE"
                     /locus_tag="VFX94_13665"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX5769277.1"
                     /translation="MPSDELTRDTIAAIATPAGRGGIGIVRLSGPRTAQIAKRILGNV
                     PQPRHATHAVFCDVRGEPLDEGIALYFPAPNSYTGEPVLELQGHGGPVVMHALLAACL
                     DAGARLAEPGEFTRRAFLEGKLDLAQAEAVADLIDAASREAARSALRSLTGDFSAALG
                     ALQSQLIELRALTEAMLDFPEEEVDALHREDAAARLGAVRAKLDELIGSSRQGSLLRC
                     GIHVVLAGRPNVGKSSVLNRLAGEERAIVTPVPGTTRDALREPVQIGGVPLVLVDTAG
                     LRVTSDLVEQLGVERTQRELEQADVVLAVYEIGGDTTPLADLPTSAARIDVYNKVDLV
                     PGFAAPPGAIAVSAKTGTGLEHLRKAILRAAGWLDRGESVFLARERHLRALEAARAHL
                     EAACAESHRWEFFAEELRRAHSALGSITGEFSADDLLGEIFARFCIGK"
     gene            1931..>2702
                     /locus_tag="VFX94_13670"
     CDS             1931..>2702
                     /locus_tag="VFX94_13670"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEX5769278.1"
                     /translation="MRPARDRWINPALALCACAAAGAVFSWLRLPLPWMVGPLAAMAL
                     CNLAGAELRSPRGGRQLGQIVIGTALGLYFTPAVAREVATYGGLLVVAGFFAILLGAL
                     CGFLLGWMAGIDRTTAFFASVAGGATEMTILGERFGARPDRVALAQSLRIVAVVLIVP
                     VALIYSGAHGTDLYQPAPLPLDAVRLALLMAIAAAAAFALNATGMPNAFMFGPLAVVI
                     ALTASDVRFSSVPPALSNAAQVLLGCTLGSRFDRRSLES"
BASE COUNT          403 a          905 c          886 g          508 t
ORIGIN      
        1 cgcgccgctg ttcctgctgc tcaagtggct gcacggcctc ctcggcaact ggggctgggc
       61 gatcgtggtg atgaccatca tgctcaaggc cgcgttctat ccgctcaatg ccgcggccgc
      121 gcgctcgatg ggcaagatga agctcatcgc gcccaagatg aaggcgctgc aggagcaata
      181 cgccaacgac aagcagcagc tgcagatcaa gatgatggag atgtacaagt cggagaagat
      241 caatccgctc ggcggctgtc ttcccatcct cgtgcagatc cccgtgttca tcgcgctgta
      301 ctgggtgctg cttgcggcaa tcgagctgcg ccacgcgccc ttcatgctgt ggatcaagga
      361 tctctcggcg cttgacccgt actacgtgct gccgatcctg atgaccggca cgatgatcct
      421 gcagacgcgg atgaatccgg tgccgcccga cccggtgcag gcgcgggtga tgcagatcat
      481 gccgttcgtc ttcagcatct tcttcttttt cttcccggcc ggactggtgc tgtactggct
      541 ggtcaacaac atcctttcca tcgcccagca atggcagatc cagcgcatgt tcgatcgcga
      601 caagcctgcc catgccaagc gatgagctga cgcgcgacac gatcgccgcg attgccaccc
      661 ccgccggtcg cggcgggatc gggatcgtgc ggctttccgg cccgcgcacg gcgcagattg
      721 cgaaaagaat cctcggcaac gtgccgcagc cgcggcacgc gacgcacgcc gtcttttgcg
      781 acgtgcgtgg cgagccgctc gacgaaggca tcgcgctgta cttcccggcg ccgaactcct
      841 acaccggcga gccggtactc gaactccagg gccatggggg cccggtagtg atgcacgcgc
      901 tgctcgctgc atgccttgac gcgggcgcgc gcctcgcgga gccgggcgag ttcacgcgcc
      961 gggcgttcct cgaaggcaag ctcgatctcg cgcaggccga ggccgtcgcc gatctcatcg
     1021 acgcggcgag ccgcgaagcc gcccgttcgg cgttgcgctc gctcacgggc gatttctccg
     1081 cggccttggg tgccttgcaa tcgcagctca tcgaattgcg cgccctcacc gaggcgatgc
     1141 tcgattttcc cgaagaggaa gtggacgcgc tgcaccgcga agacgccgcg gcgcggctcg
     1201 gcgcggtccg tgccaagcta gacgagctta tcggcagcag tcgccaggga agcctgcttc
     1261 gctgcggtat ccacgtggtc ctcgcggggc gcccgaacgt cggcaaatcg agcgtgctca
     1321 accgactggc gggtgaagaa cgcgccattg tcacgccagt cccgggaacc acccgcgacg
     1381 cattgcgcga gccggtgcag atcggcggtg tgccgctagt cctggtcgac acggctggcc
     1441 tgcgggtcac ctcggacctg gtggagcagc tcggagtcga gcgcacgcag cgcgagctcg
     1501 agcaagcgga cgtcgtattg gctgtctatg aaatcggtgg ggacaccacg ccgcttgccg
     1561 acctgcccac cagcgcggcg cgtattgatg tctacaacaa agttgatctt gtaccggggt
     1621 ttgccgcccc cccgggcgcc atcgctgtgt cggcgaagac gggcacgggg ctcgagcacc
     1681 tgcgaaaagc aatactgcgg gcagcgggct ggttggaccg cggtgaatcg gtgttcctcg
     1741 cccgagagcg tcacttgcga gcgctggagg ccgcgcgagc tcatctcgag gcagcctgcg
     1801 ccgagagcca ccgttgggaa ttcttcgccg aggagctgcg ccgcgcgcac agcgcgctcg
     1861 gctcgatcac cggggagttc agcgccgatg atctgcttgg cgaaattttc gcccgcttct
     1921 gtatcggaaa atgaggccgg cgcgcgaccg ctggatcaat ccggcgctcg cgctttgcgc
     1981 ttgcgcggcg gccggtgcgg ttttttcctg gctcaggctg ccgctcccct ggatggtggg
     2041 tccgctcgcc gcgatggcgc tatgcaatct cgccggcgcc gagcttcgct ctccacgcgg
     2101 agggcgccag ctgggacaga tcgtgatcgg cacggcgctc ggtctttatt tcacgccggc
     2161 ggtggctcgc gaggtggcca cctatggtgg cctgcttgtg gtcgccggct tcttcgccat
     2221 cctgctgggc gcgctatgtg gtttcctgct tggttggatg gcgggcatcg accgcacgac
     2281 cgccttcttc gccagcgtcg ccggcggggc gaccgagatg acgatactcg gcgaacgctt
     2341 cggtgcacgt ccggatcgcg tcgcgctcgc gcaatcgctg cgcatcgttg ccgtcgtgct
     2401 catcgtgccg gtcgcgctca tttattcggg tgcgcacggc actgacttgt accagccggc
     2461 accgctcccc ctggacgcgg tgcgcctcgc tctgctgatg gcgatagccg cggcagcggc
     2521 cttcgcgctg aacgccacag gcatgccgaa tgccttcatg ttcgggccgc tcgccgtcgt
     2581 gattgcgctg accgccagcg acgtgcgctt ctcctcggtg ccccccgctc tgtcgaacgc
     2641 tgcgcaggtt ctgctcggct gcacccttgg ctcgcgcttc gatcgccgat cgcttgagtc
     2701 ta
//
LOCUS       DASUNT010000682         4113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_525874, whole genome shotgun sequence.
ACCESSION   DASUNT010000682 DASUNT010000000
VERSION     DASUNT010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525874"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VFX94_13675"
     CDS             complement(<1..798)
                     /locus_tag="VFX94_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5769279.1"
                     /translation="MKRENVLGRANVEDTPELEAYYRSLEGEALGALWTVANEIEPWY
                     PQPKSVPTLWKWKKVEPFVRRAPELVSAEKAARRVVMLVNPGRKEWSAAVGLLYTGVQ
                     VMNPGEFTSAHRHQASALRFVMEGRGAYTVVDGERLTLGARDFVLTPNGTWHDHGVEA
                     GGTQCVWQDGLDIPLMNSLDANFYEVHPKIVQTPASPHSLPRRENWSKPYSPVFLYKW
                     DDAYKALRKEGIYEYSNPLTGGPVMPTMGARLELIKTPSQAKRHTGSV"
     gene            complement(809..1495)
                     /locus_tag="VFX94_13680"
     CDS             complement(809..1495)
                     /locus_tag="VFX94_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5769280.1"
                     /translation="MLEIRRLETSYGRSQVLFGIDLRIGKGEVVSLLGRNGMGKTTTV
                     RSIMGITQPKSGTISFGGDAIHRLPSYRVAQKGIGLVPEGRQVFPNLTVRENLVATAR
                     PGPWTLEGVFKLFPHIAARQGNYGNQLSGGEQQMLAIARALMTNPRLLILDEATEGLA
                     PLIRGEIYRSIGELKAQGLSILVIDKDVKSLTRVADRHYVLEKGRVVWSGSSKELAAD
                     TALQHRYLGV"
     gene            complement(1495..2226)
                     /locus_tag="VFX94_13685"
     CDS             complement(1495..2226)
                     /locus_tag="VFX94_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5769281.1"
                     /translation="MLEVRGLTKNFGALRASDSIDFDVAEGETHAVIGPNGAGKTTFI
                     SQLAGNLRPDAGVIRFAGEDVTRLPAHARARKGFARSFQITSVFPDFSALHNVALAVQ
                     AQLGRAGFWRPARSDPALVGPARELLREVGLGHRADALAANMSHGEHRQLEVAMALAT
                     KPRLLLLDEPMAGMGVEESQRMIELLSRLKQAKTIILVEHDMDAVFRLADRISVLVYG
                     RVIATGPPEQIRANQEVRKAYLGEA"
     gene            complement(2219..3148)
                     /locus_tag="VFX94_13690"
     CDS             complement(2219..3148)
                     /locus_tag="VFX94_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5769282.1"
                     /translation="MSTRLVLWTISVLALVALPPFVLYIGQPFYLDLVRRIMILAIAA
                     ISLNLILGYGGLVSFGHAAYLGVGAYAVGILGFYGFTSGWLHLAVAVVASALVAAAIG
                     AVSIRTSGIYFIMITLAFSQMLYYLGISIEEFGGDDGMRIKVQSQFPLVDLANPLVFY
                     YVALALMLLSVFAVFRIVNSRFGMTLRAAKSNEARARAIGFSPYPYRLAAFVIAGAMC
                     GLAGMLYANHTRYITPDLASWQQSGDIMFMVILGGMASTAGPVLGTFALLLVEELLKG
                     WTEHWQVVLGPLLVLSVIFFRRGLAGVFYKPDA"
     gene            complement(3145..4065)
                     /locus_tag="VFX94_13695"
     CDS             complement(3145..4065)
                     /locus_tag="VFX94_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5769283.1"
                     /translation="MPWILVFEQLLNGVQLGVTLFLMAAGLTLVLGIMNLVNLAHGSL
                     YMLGAYLTLAASQWTGSYLAGVALGLAGTLVLGMLIEVITLRALYDRDHLDQVLATFG
                     LILFFNELVAIIWGRASLYAQLPEWLKGHVEIFTGSSYPVYRLAVIAVGLLVGIALWY
                     VVARTRLGMLIRAGASNRTMVAALGVNIRLLYTVVFGFGAALAGLAGVMAGPIYSVQP
                     GMGELILIQVFVVIVIGGIGSVRGAFVGALIVGMVDTLGRAFLRPALGAMISPTAGDS
                     AGPALASMLIFVLMAVVLTFRPQGLFPPRG"
BASE COUNT          759 a         1342 c         1369 g          643 t
ORIGIN      
        1 gacgcttccc gtatggcgtt tcgcttggga aggcgtcttt atcaattcaa ggcgcgcacc
       61 catcgtcggc atgaccgggc cgccggtcag cgggttggaa tactcgtaaa tcccttcttt
      121 gcggagagcc ttgtaggcgt catcccattt gtaaagaaag accggtgagt agggcttcga
      181 ccagttctcg cgccgaggta aggaatgcgg cgacgccggc gtctgaacga tcttcggatg
      241 cacctcgtag aagttcgcgt cgagcgagtt catgagcggg atgtccagac cgtcctgcca
      301 gacgcattgc gtgccgccgg cctcgacacc gtggtcgtgc caggtgccat tcggcgtgag
      361 gacgaaatcg cgcgccccga gcgtcaagcg ctcgccgtct acgacggtgt acgcgccgcg
      421 gccttccatg acgaaacgca gcgcgctcgc ctgatgtctg tgagccgacg tgaactcgcc
      481 gggattcatg acctgcacgc cggtgtagag caggccgacc gcggcgctcc attccttgcg
      541 gcccgggttg acgagcatca ctacgcgccg cgccgccttt tcggcggaaa cgagttccgg
      601 cgcgcgccgc acgaacggct ccactttctt ccacttccag agtgtcggaa ccgatttcgg
      661 ctgcgggtac cacggctcga tctcgttcgc caccgtccac agggcgccga gcgcctcgcc
      721 ttccaggctg cggtagtagg cttcgagctc cggcgtgtct tcgacgttcg cgcgcccgag
      781 gacgttctcg cgcttcatgg cggaaagtct acaccccgag atatcggtgc tgcagggcgg
      841 tatccgccgc gagctccttg gagctgccgc tccagaccac gcgccctttt tcgagcacgt
      901 agtggcggtc cgcgacccgg gtcagcgact tcacgtcctt gtcgatgacg aggatcgaca
      961 gcccttgcgc cttgagctcc ccgatcgaac ggtaaatttc gccgcgaatg agcggggcga
     1021 ggccttcggt cgcttcgtcc aggatcagga ggcgcgggtt ggtcatgagc gcgcgcgcga
     1081 tggcgagcat ctgctgctcc ccgcccgaga gctggttgcc gtagttgccc tggcgcgccg
     1141 cgatgtgcgg aaagagcttg aacacgcctt cgagggtcca tggcccgggt cgcgcagtgg
     1201 cgaccaggtt ctcgcgcacc gtgagattcg ggaaaacctg ccgtccctcg ggcaccaggc
     1261 cgattccctt ttgcgctacg cggtaggacg gcaggcggtg gattgcgtcg ccgccgaacg
     1321 aaatcgtgcc cgacttcggc tgcgtgatgc ccatgatcga gcgtacggta gtggtcttgc
     1381 ccatgccgtt acggccgagg agcgatacca cctcgccctt cccgatgcgc aggtcgatgc
     1441 cgaacagcac ctgactgcgg ccgtaggagg tctcgaggcg gcggatctcg agcatcaagc
     1501 ttcgcccagg tacgctttcc gtacctcttg attcgcccgt atctgctcgg gcgggccggt
     1561 ggcgatgacc cggccgtaca ccagcaccga gatgcggtcg gcgaggcgga acaccgcgtc
     1621 catgtcgtgc tcgacgagga tgatggtctt cgcctgcttc agccgcgaga gcagctcgat
     1681 catgcgctgc gattcctcga ctcccatgcc ggccatcggc tcgtcgagca gcagcagccg
     1741 cggcttcgtg gcaagcgcca tggccacttc gagctgacgg tgctcgccgt gcgacatgtt
     1801 cgccgccagg gcatcggcgc gatgtcccaa tcccacttcc cgcagaagct cgcgcgcagg
     1861 tccgacaagc gccgggtcgc tgcgcgcggg acgccagaac cccgcgcggc cgagctgcgc
     1921 ctggacggcg agagcgacgt tgtgcagggc gctgaagtcc gggaaaacgc tggtgatctg
     1981 gaacgagcgc gcgaagccct tgcgcgcgcg cgcatgtgcg ggcaggcgcg tcacgtcctc
     2041 gccggcgaag cggatcacgc cggcgtcagg ccgcaggttg ccggcgagct ggctgatgaa
     2101 ggtcgtcttg ccggcgccgt tcgggccgat tacggcgtgc gtctcgcctt ccgctacgtc
     2161 gaagtcgatg ctgtcgcttg cgcgcagcgc gccaaagttc ttcgtcagtc ctctgacctc
     2221 aagcatcggg cttgtagaaa accccggcga gcccgcgccg gaagaaaatg accgaaagca
     2281 cgagcagcgg cccgaggacg acctgccaat gctcggtcca tcccttgagc agctcctcga
     2341 cgaggagcag ggcgaacgtg ccgagcaccg gccccgcggt gctcgccatg ccgccgagga
     2401 tcaccatgaa catgatgtcg cccgactgct gccagctcgc gaggtcgggc gtgatgtagc
     2461 gcgtatggtt ggcgtacaac atcccggcga ggccgcacat ggcgcccgca atgacaaacg
     2521 ccgccaggcg gtacgggtac ggcgagaagc cgatcgcgcg ggcgcgggct tcgttcgact
     2581 ttgccgcgcg cagcgtcatg ccgaatcgcg agttgacgat cctgaatact gcgaaaacgg
     2641 acaggagcat cagcgcgagc gcgacgtagt agaacaccag cgggttcgcc aggtcgacga
     2701 gcggaaactg gctctgcacc ttgatccgca tgccgtcgtc gccgccgaac tcctcgatcg
     2761 agataccgag gtagtacagc atctggctga aggcgagcgt gatcatgatg aagtagatgc
     2821 cgctcgtgcg gatcgacacc gcgccgatcg ctgcggcgac cagcgccgag gcgacgaccg
     2881 cgacggccag gtgcagccag ccgctggtga agccgtaaaa gccgaggatg ccgaccgcgt
     2941 aggcgcccac gccgaggtag gcggcatggc cgaaggacac gaggccgccg tagccaagga
     3001 tcaggttcag gctgatggcg gcaatggcga ggatcatgat ccgccgcacg aggtcgaggt
     3061 agaacggctg gccgatgtaa agcacgaaag ggggcagcgc gacaagcgcg agcaccgaaa
     3121 tggtccaaag gacgaggcgg gtgctcatcc gcgcggcgga aacaggccct gcggccggaa
     3181 cgtaagcacc accgccatca gcacgaagat gagcatcgag gcgagcgccg ggccggcgga
     3241 gtcgccggcg gtcggggaga tcatcgcgcc gagcgccggc ctgagaaacg cgcgccccag
     3301 ggtatcgacc atgccgacga tcaacgcgcc gacgaacgcg ccgcgcaccg agccgatgcc
     3361 gccgatcacg atcacgacga acacctggat caggatgagc tcgcccatgc cgggctgcac
     3421 cgaatagatc ggacccgcca tcaccccggc gagtccggcg agcgcggcgc cgaatccgaa
     3481 caccaccgtg tagagcagcc ggatattcac gccgagcgcg gccaccatgg tgcggttcga
     3541 ggcccccgcg cggatcagca tcccgagccg cgtgcgcgcc acgacgtacc aaagcgcgat
     3601 cccgacgagc aggccgacgg cgatgaccgc gaggcgatac accggatagc tcgagccggt
     3661 gaagatctcg acatgccctt tcagccactc cggaagctgc gcatacaagg acgcccggcc
     3721 ccagatgatg gcgacaagct cgttgaagaa caggatcagg ccgaacgtgg cgagcacctg
     3781 gtcgagatgg tcgcggtcgt agagcgcgcg cagcgtgatg acctcgatca gcatgccgag
     3841 cacgagcgtg ccggcgaggc cgagggcgac gccggcgagg taagagcccg tccactgcga
     3901 cgccgcgagc gtgagatagg cacccaacat gtagagcgag ccgtgcgcca ggttcacgag
     3961 attcatgatg cccagtacga gcgtcagccc cgccgccatg aggaacagcg tcacccccag
     4021 ctgcacgccg ttcaggagct gctcgaagac caggatccac ggcataaaaa agaaagggcg
     4081 cggtttgcgc gcccttcgtt ttacgctcct tgc
//
LOCUS       DASUNT010000683         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_700260, whole genome shotgun sequence.
ACCESSION   DASUNT010000683 DASUNT010000000
VERSION     DASUNT010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700260"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..517)
                     /locus_tag="VFX94_13700"
     CDS             complement(188..517)
                     /locus_tag="VFX94_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769284.1"
                     /translation="MKPAPLGALNAELGPIEEDLAARIDVIFERCPQLFGFTVQDSSV
                     LPEELRSLSLERELVVSDIGVYPYLNPDQCEQIYNEIAVALLDFIFERPAAKDVLRGR
                     TFVRTLH"
     gene            563..1810
                     /locus_tag="VFX94_13705"
     CDS             563..1810
                     /locus_tag="VFX94_13705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5769285.1"
                     /translation="MADKKIFYGWWMVGAATGLQFLQAGLVTQAFGAYLAVLADEQGW
                     SKTALSGAAALQQMEVALLGPVLGWLIDRFGPKVFVRAGIVVFGSGLCLLSQVQTLPA
                     FYGAFVVIALGSSLCGFFPLNVALIHWFERKRARALSAMSIGLAFGGIIVPLVAQSLT
                     TFGWRATALASGLLAIAVGMPLALVIRNSPRDHGETVDGISEEKSSSETTRESETPPA
                     TRDFTAREALRTQAFWLISLGHGFALFVVHAVSVHAITHLKEGLGYSGAAASLVISLV
                     TTCQIGGVLLGWAIGDRYRKRLIAAGCMLAHAAGLLLLTYAVNTAMVVAFAVLHGTAW
                     GLRGPMMQAMRADYFGRSSIGMILGLSFMIIVVGQVGGPVIPGIFADITGNYRLGFTI
                     IALLAGLGSGFFFLARKPKRPAA"
     gene            complement(1807..>2093)
                     /locus_tag="VFX94_13710"
     CDS             complement(1807..>2093)
                     /locus_tag="VFX94_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEX5769286.1"
                     /translation="AIADARSICAEDLLVAERRYPHRVGETYRLRHNPGHRWFYFPRM
                     RRDEALVFKVYDSERDGRARFTPHTSFVDPGTPPGAPPRQSIEARTLVFF"
BASE COUNT          325 a          695 c          707 g          366 t
ORIGIN      
        1 gaccagatcg atacccggcg ctgggggccg ggcgagcgca tcgtcttcga gccgtatacg
       61 cgcgaggtgt tcgagcagtc gttcaagtgg atcgccgagc gcgagatctt caagccgggc
      121 gagatgggac cggggcgcta cgaagacgct tgtgtttcgc taagctgaaa agggggtcag
      181 ttcgagctta gtggagagtc cggacgaagg tccggccgcg cagcacgtcc ttcgccgccg
      241 gccgctcgaa gatgaagtcg agcagggcca ccgcgatctc gttgtagatc tgctcgcact
      301 ggtcggggtt cagataggga tagacgccga tatcgctcac taccagctcg cgctcgagcg
      361 acagcgagcg cagctcttcg ggcagcaccg aggagtcctg caccgtgaag ccgaaaagct
      421 gcgggcagcg ctcgaagatc acatcgatgc gggcggccag gtcttcctcg atcggcccca
      481 gttcggcatt aagagcgcct agcggtgcgg gtttcatgcc ggcgctgccg caagtaacgg
      541 acccgacgct atgctgtcgg ggttggccga caagaaaatc ttctacggat ggtggatggt
      601 cggcgcggcg accggcctgc agttcctgca ggccggactc gtcacgcagg ccttcggcgc
      661 ctacctggcg gtgctcgccg acgagcaggg ctggagcaag accgcgctct ccggcgcggc
      721 ggcgctccag caaatggagg tcgcactgct cggcccggtg ctcggctggc tgatcgaccg
      781 cttcggcccg aaggttttcg tgcgcgccgg catcgtggtg ttcggcagcg ggctttgcct
      841 gctctcgcag gtgcagacgc tgcccgcgtt ctacggcgcg ttcgtcgtca tcgcgctcgg
      901 gtcgagcctg tgcggcttct ttccgctcaa cgtcgcgctc atccactggt tcgagcgcaa
      961 gcgcgcccgc gcgctctcag cgatgtcgat cgggctcgcc ttcggcggca tcatcgtccc
     1021 gctcgtcgcg cagtcgctca ccacgttcgg ctggcgcgcc accgccctcg cctcgggcct
     1081 gctcgcgatt gccgtcggca tgccgctcgc cctggtgatc cgcaacagcc cgcgcgacca
     1141 cggcgagacg gtcgatggaa taagcgaaga aaagtcctcg agcgaaacga cccgggagag
     1201 cgaaaccccg ccggcaacgc gtgacttcac cgcccgcgag gcgctgcgta cgcaggcctt
     1261 ctggctcatc tcgctcggcc acggcttcgc gctcttcgtg gtgcacgcgg tgtcggtgca
     1321 tgccatcacg cacctcaagg aaggcctcgg ctactcgggg gcggccgcct cgctcgtcat
     1381 aagcctggtg acgacatgcc agatcggcgg cgtgctgctc ggctgggcga tcggcgaccg
     1441 ctaccgcaag cgcctgatcg ccgcgggctg catgctggcg cacgccgcgg ggctgctgct
     1501 gctgacctat gccgtgaaca cggcgatggt ggtcgccttc gccgtgctgc acggcaccgc
     1561 gtggggcctg cgcgggccca tgatgcaggc gatgcgcgcc gactacttcg gacgaagctc
     1621 catcggcatg atcctcggcc tgtcgttcat gatcatcgtg gtaggccagg tcggcgggcc
     1681 ggtgatcccg ggaatcttcg ccgatatcac cggcaactac cgcctcggct tcactatcat
     1741 cgcgttgctc gccggtctcg gctcgggctt ctttttcctc gcgagaaagc cgaaaagacc
     1801 ggccgcctag aagaaaacga gcgtcctcgc ttcgatgctc tggcgcggcg gggcgcccgg
     1861 cggcgtgccc ggatccacga acgaggtatg cggcgtgaag cgcgcacggc cgtccctttc
     1921 cgagtcgtac accttgaaca ccagcgcctc gtcgcgccgc atgcgcggga agtagaacca
     1981 gcgatgaccc gggttgtgcc tgagacgata cgtttcgccc acgcggtgcg gatagcgtcg
     2041 ctcggcgacg agcaggtcct cggcgcagat gctgcgcgca tccgcgatcg cga
//
LOCUS       DASUNT010000684         4228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_788152, whole genome shotgun sequence.
ACCESSION   DASUNT010000684 DASUNT010000000
VERSION     DASUNT010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788152"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VFX94_13715"
     CDS             complement(<1..803)
                     /locus_tag="VFX94_13715"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5769287.1"
                     /translation="MSGNPLDLRPWLVEVEKLGELKHVEGADWNLELGAISELNVKKD
                     APPALLFDRIMGYPKGFRVLTCSTSSPARLSSILRLPIQRDHAGLVAALRGKPAQWQA
                     AAKDFPCEVVKSGPVLENVREGAGVDVHAFPSPLWHELDGGRYIGTGCSVVTRDLDSD
                     WVNVGTYRVQVLDGKHVALDMVPGKHGRIQYEKHQKAGKRFPVAIVVGGDPLGYLIAG
                     IEIPFGMCEWNYVGAILNQPVSVVLGTQTHLPFPAASEIVLEGFVEPND"
     gene            831..1781
                     /locus_tag="VFX94_13720"
     CDS             831..1781
                     /locus_tag="VFX94_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5769288.1"
                     /translation="MRLLAALCIALVSFPAVPQPYPSRPVRMVVGFPPGGGTDIMARV
                     LAPRLSEYLGQAFVVENRPGATTNIASDVVAKSAPDGYTLLFTTSALAINMSLYRNLP
                     FDALRDFAPISVFAESPNLLVAHSSAGASVHELLAQARAKPGAMNYSSAGAGTSQHLA
                     GELFKVRTATQIAHIPYKGTAASLTAVIAGEVHFSFANVPAILGHVRSGRLRPLAVAG
                     PKRSDLMPDVPTMQEAGVAGVEVPVWYALLGPAGLPFDLINTLNSATVRAARSPDLRQ
                     KLIEQGAEPVGNTPEAFSTLLREEVEKWAEVVRISGAKAE"
     gene            1867..2703
                     /locus_tag="VFX94_13725"
     CDS             1867..2703
                     /locus_tag="VFX94_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769289.1"
                     /translation="MKIQLAHGMAALLAAVSLQTAAQEVIRPGQEKWTIMLGAFLPAF
                     ETKMKVDNDQLGSGDNVDLGDDLGVDQDESGGWFGVEWRFAPRHRLGFTYSRFTLNGE
                     RTLTRDLQIGDEIFPAGANVSSQLRLTIMPITYSYSFLKRERDELALTLGLHWSSLRF
                     SAAGSASLGTQDFSRDSNTDADVPLPLLGLRYDHHFSDRWSAGASAAVFALEFGQDTW
                     NFEGHLWSVRLHAEYRFARNFAIGAALDGFDVSVNLSSGSWTGGFDYGYWGPQIYLTA
                     RF"
     gene            complement(2706..4136)
                     /locus_tag="VFX94_13730"
     CDS             complement(2706..4136)
                     /locus_tag="VFX94_13730"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5769290.1"
                     /translation="MKRLLIALAAVASLGHAQADEALLEAMAKPWSGDLDGMIERRAV
                     RALVAPSRMQYWIDRGRQSGAEYELLRKFEEVLNTKYKSKRKHIRINVHFIPTARDEL
                     IPALLQGRGDIAAGILTVTPERLTQVDFGAPFFRNVAEIVVTGPASPPLASIEDLAGQ
                     KLFARRSSSYWTHLEELNARLRQQGRAPFELKTAPEDLQDDDLLEMLNAGLVGLAVVD
                     RYKATTWARVFKRIKPRDDLRVHEGGDIAWMIRKDSPRLKAEISAFAEQHGQGSAFGN
                     SLFKKYTGSSRFVRAATSKEEMRKFDQTVELFRKYSDKYEMDYLLMMAQGYQESRLDQ
                     KAKSPVGAIGVMQVMPDTGKDMKTGDITQLEPNIHAGVKYIRYVQNTFFESQPMDAVN
                     KTLFSFAAYNAGPGRVQDLRARAQKRGLNPNVWFNNVELIAAERIGAETVTYVSSIYK
                     YYVAYKLAVEIQAERARERDALKKKP"
BASE COUNT          677 a         1433 c         1349 g          769 t
ORIGIN      
        1 tcgtcgttcg gctcgacgaa gccctccagc acgatctccg aggcggcggg aaacggcagg
       61 tgcgtctgcg ttccgaggac gaccgagacg ggctgattca agatggcgcc gacatagttc
      121 cactcgcaca tgccgaacgg aatctcgatc cccgcgatca gatacccgag cgggtcgccg
      181 ccgaccacga tcgccaccgg gaagcgcttg ccggccttct ggtgcttctc atactggatg
      241 cggccgtgct tgccaggaac catgtccagg gcaacgtgct ttccatcgag cacctgaacc
      301 cggtaggtgc cgacgttcac ccagtccgag tcgaggtcgc gcgtcaccac cgagcagccg
      361 gtgccgatgt agcgcccgcc atcgagctca tgccagagcg gcgaggggaa cgcgtgcacg
      421 tcgactccgg cgccttcccg aacgttctcc aggaccggcc cgctcttcac cacctcgcag
      481 ggaaagtcct tcgccgccgc ctgccactga gccggcttgc cgcgcagcgc ggcaaccagt
      541 ccggcatgat ctctttgaat cggcaacctg aggatcgacg acaggcgcgc cgggctgctc
      601 gtgctgcagg tcagcacgcg aaagcccttc gggtatccca tgatgcggtc gaagagcaac
      661 gcgggcggcg cgtccttctt cacgttcagc tcgctgatcg cgccgagctc gaggttccag
      721 tccgcgccct cgacatgctt gagctcgccc agcttttcca cttccacgag ccacggccgc
      781 agatcgagag gattcccgct catcgcggcg cattataaga ttcggcggcc atgagacttc
      841 tcgccgcact gtgcatcgcg ctcgtttcat ttccggcggt gccccaaccc tatcccagcc
      901 gcccggtgcg catggtcgtc ggcttcccgc ccggcggggg caccgacatc atggcccgcg
      961 tgctggcgcc gcgcctatcg gagtacctgg ggcaggcctt cgtcgtcgag aaccggcccg
     1021 gcgccaccac gaacattgcg agcgatgtgg tggcgaagag cgcgccggac gggtacacgc
     1081 tgctcttcac cacgtctgcg ctcgcgatca acatgtcgct ctacaggaac ctgccgttcg
     1141 acgcgctgcg cgattttgcg ccgatctcgg tgttcgccga gagcccgaac ctgctcgtgg
     1201 cgcattcgag cgccggcgcg agtgtccacg agctgctcgc gcaggcgagg gcgaagcccg
     1261 gcgcgatgaa ctactcttcc gccggcgcgg gtacgagcca gcatctcgcg ggcgagctct
     1321 tcaaggtgcg caccgcaacg cagatcgcgc acatcccgta caaaggcacg gcggcttctt
     1381 tgaccgccgt catcgccggc gaagtgcact tcagcttcgc caacgtcccg gccatcctgg
     1441 ggcacgtacg aagcggaagg ctcaggccgc ttgcggtggc gggaccgaag cgctcggacc
     1501 tcatgcccga cgttcccacc atgcaggaag ccggtgtcgc cggcgtcgaa gtgccagtgt
     1561 ggtacgccct gctcggccct gccgggctgc cgttcgatct catcaacacc ctgaacagcg
     1621 ccaccgtgcg cgcagcgcgc tcgccggatc tgcggcaaaa gctcatcgag cagggagccg
     1681 agcccgtcgg caatacgccc gaggcgttct caacactcct gcgcgaggaa gtggagaagt
     1741 gggccgaggt cgtgcgcatt tccggcgcaa aggcggaata ggccccaact gatcgcaagc
     1801 gggcgagccc ctgtttgcgc ggaacgcctc ccgcctctaa cgtatggctt catagggagc
     1861 tcgttgatga agatccaact tgcgcacggg atggcggcac tgctcgccgc cgtgagcttg
     1921 cagacggccg cacaggaggt gatccgtccc gggcaggaga agtggacgat catgctgggc
     1981 gccttcctgc ccgcgttcga gaccaagatg aaggtcgata acgaccagct cgggtcgggc
     2041 gacaacgtgg atctcggcga cgacctgggc gtggaccagg acgagtcggg cggctggttc
     2101 ggcgtcgagt ggcgcttcgc gccgcgtcat cggttgggct ttacttacag ccgcttcacg
     2161 ctgaacggtg agcgcacgtt gacccgcgac ctgcagatcg gcgacgagat ctttcccgcc
     2221 ggcgccaacg tgtcgtcgca gctgcggctc acaatcatgc cgatcacata ctcatactct
     2281 ttcctgaagc gcgagcgcga cgagctcgcg ctgacgctcg gcctgcactg gagtagcctg
     2341 cgcttcagcg cggcgggatc cgcctcgctc ggcacgcagg atttctcgcg cgactccaac
     2401 acggatgcgg acgtgccgct gccgctgctt ggcttgcgct acgaccacca cttctccgac
     2461 cgctggtccg caggggcgag cgccgccgtg tttgccctgg agttcggcca ggacacgtgg
     2521 aacttcgaag gccacctgtg gagcgtgcgg ctgcacgccg agtaccgctt cgcgcgcaat
     2581 ttcgccatcg gcgccgcgct cgacggcttc gacgtcagcg tgaacttgag ctccggcagc
     2641 tggacaggcg gcttcgacta cggctactgg gggccgcaga tctacctcac cgcgcgcttc
     2701 tagcgctacg gctttttctt gagcgcgtcg cgctcccgcg cgcgctcggc ctggatctcg
     2761 accgcgagct tgtaggccac gtagtacttg tagatgctcg aaacgtaggt cacggtctcc
     2821 gcgccgatcc gctcggcggc gatcagctcg acgttgttga accagacgtt cggattcagc
     2881 ccgcgcttct gggcgcgagc gcgcaggtcc tgcacccggc ccggcccggc gttgtaggcc
     2941 gcaaaggaga acagcgtctt gttgaccgcg tccatcggct ggctctcgaa gaacgtgttc
     3001 tggacatagc ggatgtactt gaccccggcg tggatgttcg gctcgagctg agtgatgtcg
     3061 ccggtcttca tgtccttgcc cgtgtcgggc attacctgca tcaccccgat ggcgccgacc
     3121 gggctcttcg ccttctggtc gaggcgcgac tcctggtagc cctgcgccat catcaggaga
     3181 tagtccattt cgtacttgtc gctgtacttg cggaagagct caacagtctg gtcgaacttg
     3241 cgcatctctt ccttcgaggt cgcggcgcgg acgaagcggc tgctgccggt gtacttcttg
     3301 aacaggctat tgccaaaagc gctgccctgc ccgtgctgtt cggcaaacgc cgaaatctcc
     3361 gccttaaggc gcgggctgtc cttgcggatc atccaggcga tgtcgccgcc ttcgtgcacc
     3421 ctcaggtcgt cgcgcggctt gatgcgcttg aaaacgcgcg cccaggtggt ggccttgtat
     3481 cgatccacca ccgccagtcc gaccaggccg gcgttcagca tctccagcag gtcgtcgtcc
     3541 tgcaggtcct cgggcgcggt cttcagctcg aagggcgcgc ggccctgctg acgcagacgt
     3601 gcgttcagct cctcgaggtg cgtccagtag ctcgacgagc ggcgcgcgaa gagcttctgc
     3661 ccggcgagat cctcgatcga tgcgagcggc ggcgaggccg ggccggtcac cacgatctcg
     3721 gcgacgttgc ggaagaacgg cgcgccgaag tccacctgcg tcagccgctc cggcgtgacc
     3781 gtgaggatgc cggcggcgat gtcgccgcgg ccctgcagga gcgcagggat cagctcgtcg
     3841 cgcgccgtcg ggatgaaatg cacgttgatt cggatgtgct tgcgcttcga cttgtacttg
     3901 gtgttcagca cttcttcgaa cttcctgagc agctcgtact cggcgccgct ctggcggccg
     3961 cggtcgatcc agtactgcat gcgcgaaggc gccaccagcg cgcgtaccgc gcgccgctcg
     4021 atcatgccgt cgaggtcgcc gctccatggc ttcgccatcg cctcgagcag cgcctcgtcg
     4081 gcttgggcgt gaccgagact cgccacggct gcaagcgcaa tcaacaggcg tttcactctc
     4141 ggatctcctc gagcaccggg cgcaggtagc cgaggaacgc ttcggcgttc tcgctcatgg
     4201 gaaagtggcc caggccttgc attaccgt
//
LOCUS       DASUNT010000685         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_875627, whole genome shotgun sequence.
ACCESSION   DASUNT010000685 DASUNT010000000
VERSION     DASUNT010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875627"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VFX94_13735"
     CDS             <1..593
                     /locus_tag="VFX94_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5769291.1"
                     /translation="RRCEKPLNVYGYSKFLFDQYVRRMLERKNAQVAGLRYFNVYGPN
                     EQHKGRMASVAYHAYQQLLVAGKVKLFVGSGGYDDGEQRRDFVYVEDVVDVNLWLLER
                     RDVSGVFNCGTGRAQTFNQLAAAVINAVQGTRLTVQEMVQKGLIEYIPFPAGLADKYQ
                     SYTQADLTRLRAAGYPGEFLSVEQGVAAYVGELQKR"
     gene            590..1477
                     /gene="cysM"
                     /locus_tag="VFX94_13740"
     CDS             590..1477
                     /gene="cysM"
                     /locus_tag="VFX94_13740"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HEX5769292.1"
                     /translation="MKKTVEDFIGNTPLVRLQRLPGSERNVLLAKLEGNNPAGSVKDR
                     PAISMVRHAERRGEIKPGDTLIEPTSGNTGIALAMAAAIRGYRMVLIMPEHLSAERRQ
                     TMRAFGAEILLTPREGGMEAARDLAERMVREKKGTMLDQFANPDNPRVHYETTGPEIW
                     RETEGRITHFVSSMGTTGTIMGCSRFFKEKNARIQIIGCQPQEGSQIPGIRKWPEAYL
                     PKIYDKSRVDRLEHVSQAEAEDMTRRLAREEGIFAGMSSGGAVHVALRVSKELKHAVI
                     VTVICDRGDRYLTTGVFPD"
     gene            1547..2323
                     /locus_tag="VFX94_13745"
     CDS             1547..2323
                     /locus_tag="VFX94_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5769293.1"
                     /translation="MTPILAFDIETVPDVAGIRRLYELPADLPDHDVAEIALQKRRVQ
                     SGVDFLPPHLHRIVAIGCVLREDDAVQVFSIGEPERDEAATIQKFFDGIDKDTPQLVS
                     WNGSSFDLPVLGYRTLLCGGCATKFWDQGADDPDFRYNNYVSRYHARHCDLMDVLAMY
                     QPRNNAPLDQVAQLAGLPGKLGIGGPAVWAAYLKGEIRKIRDYCEGDCVNTYLLYLRF
                     QRQRGILTQEQHDKETELLRSLLQATGKPYWQEFLALWKT"
BASE COUNT          439 a          758 c          771 g          358 t
ORIGIN      
        1 agcgccgctg cgagaagccg ctcaatgtgt acggctactc gaagtttctg ttcgatcagt
       61 acgtaagaag gatgctggaa aggaagaacg cccaggtggc cgggttgcgc tacttcaatg
      121 tgtacggccc gaacgagcag cacaaagggc gcatggcctc ggtcgcctac cacgcgtacc
      181 agcagctcct ggtcgccggc aaggtgaagc tcttcgtcgg ctcgggcggc tacgacgacg
      241 gcgagcagcg gcgcgacttt gtctacgtgg aagacgtggt ggacgtgaac ctgtggctgc
      301 tcgagcgccg cgatgtctcg ggcgtgttca actgcggcac cgggcgcgcg cagaccttca
      361 accagctcgc cgcggcggtg atcaacgcgg tgcaggggac gcggctcacg gtccaggaga
      421 tggtgcagaa gggcctgatc gaatacatcc cgttcccggc cgggctcgcc gacaagtacc
      481 agagctacac ccaggccgat ctcacgcgcc tgcgagccgc gggctacccg ggtgaattcc
      541 tatcggtcga gcagggcgtc gccgcctacg tcggcgagct gcagaagaga tgaagaagac
      601 cgtcgaggac ttcatcggta acacgccgct cgtgcggctg cagcgcttgc ctggctctga
      661 gcgcaatgtt ctcctcgcaa agctggaggg caacaatccc gccggctcgg tcaaggaccg
      721 gccggcgatc tcgatggtcc gccacgccga gcggcgcggc gagatcaagc ccggcgacac
      781 gctgatcgag cccacctcgg gcaacaccgg catcgcgctc gccatggcgg cggcgatccg
      841 cggataccgc atggtgctca tcatgccgga gcacctgtcg gccgaacgtc gccagacgat
      901 gcgagcgttc ggcgccgaga tcctcctcac gccgcgcgaa ggcggcatgg aggcggcgcg
      961 cgatctcgcc gagcgcatgg tgcgcgagaa gaagggcacg atgctcgacc agttcgcgaa
     1021 ccccgacaac ccgcgcgtgc attacgagac caccggaccg gagatctggc gcgagaccga
     1081 agggcgcatc acgcacttcg tgtcgagcat gggtacgacc ggcaccatca tgggctgctc
     1141 gcgcttcttc aaggagaaga acgcgcgcat tcagatcatc ggctgccagc cgcaggaggg
     1201 ctcgcagatc cccggcatcc gcaagtggcc cgaagcctac ctgccgaaga tctacgacaa
     1261 gtcgcgcgtc gaccggctcg agcacgtgtc gcaggccgag gcggaggaca tgacccggcg
     1321 cctggcgcgc gaggagggca tctttgccgg catgtcctcg ggcggggcgg tgcacgtggc
     1381 gctgcgcgtg tcgaaggagc tgaagcacgc ggtcatcgtc accgtcatct gcgatcgcgg
     1441 cgaccgctat ctgacgaccg gggtgttccc tgactagaac aatcggggtc agaccccaac
     1501 aagatagaac caagaagaat cggggtcaga gcccgatttt tcggccgtga ctccgatcct
     1561 cgccttcgac attgagaccg tgccggacgt cgcgggcatc cgccgcctgt acgagctgcc
     1621 cgccgacctg ccggaccacg acgtggccga aatcgcgctg cagaagcgcc gcgtgcagag
     1681 cggagtcgac ttcctgccgc cgcatcttca ccgcatcgtc gcgatcggct gcgtgctgcg
     1741 cgaggacgac gcggtgcagg tattctcgat tggcgagccc gagcgcgacg aggccgcgac
     1801 gatccagaag ttcttcgacg gcatcgacaa ggacacgccg cagctcgtgt cctggaacgg
     1861 ctcgagcttc gacctgccgg tgctcggtta ccgcacgctg ctgtgcggcg gctgcgcgac
     1921 caagttctgg gatcagggcg ccgacgaccc ggacttcagg tacaacaact acgtgagccg
     1981 ctatcacgcg cggcattgcg acctgatgga cgtgctcgcc atgtaccagc cgcgcaacaa
     2041 cgcgccgctc gaccaggtgg cgcagctcgc cgggctgccg ggcaagctcg gcatcggcgg
     2101 accggcggtg tgggcggcct acctcaaggg cgagatcagg aagatccgcg actactgcga
     2161 gggagactgc gtcaacacct atcttcttta cctccggttc cagcgccagc gcgggatcct
     2221 gacgcaggag cagcacgaca aggaaaccga gctgctccgc tccttgctcc aggcgaccgg
     2281 caagccgtat tggcaggaat ttctcgcact ctggaagacc tgattc
//
LOCUS       DASUNT010000686         2981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_86912, whole genome shotgun sequence.
ACCESSION   DASUNT010000686 DASUNT010000000
VERSION     DASUNT010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86912"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VFX94_13750"
     CDS             complement(<1..853)
                     /locus_tag="VFX94_13750"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX5769294.1"
                     /translation="MQKKLLSLAVAGALAAPAAALAQVEVYGTIHMSLNYMQYGEGTG
                     GVPSVSKFDVASHASNIGVRARENLGGGLSAWAQVETNASMERSNNVAHTSNFASRNS
                     AVGLQGNWGNLFVGQWTTPWADLDALWGIGTVATYGPITSIIGRRETTGSAPNPNCGN
                     TGAGVGGVTSCDSVEGGGGVGHPFWRRISNSVHYQSPVFGGAQVKVAYQTNQDKASTG
                     VTADPQMWSAAVTWAGMGGRLRVGAAFDTHMDFTSVGNDDTGWRVTGGYNFGFMDIGF
                     AYEQMTYK"
     gene            complement(1047..1820)
                     /gene="trpC"
                     /locus_tag="VFX94_13755"
     CDS             complement(1047..1820)
                     /gene="trpC"
                     /locus_tag="VFX94_13755"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX5769295.1"
                     /translation="MSILERILAAKRAEVVAAKEKIPEKEIRARAATATPARDFVGAL
                     RSKRPAVIAEIKRASPSKGLLREDFDPAAIARSYEQAGAACMSVLTDPQFFQGSSTHL
                     QQARAACALPALRKDFLIDPYQAHESRALGADCVLLIAACLGDAQMRELEDLALELGM
                     AVLVEVHDAGELERALALKTPLVGINNRSLRTFETRLETTLELLPRVPNDRIVITESG
                     IRAPADVARMRDAGVHTFLVGEAFMRASDPGFALRSLFF"
     gene            complement(1817..2857)
                     /gene="trpD"
                     /locus_tag="VFX94_13760"
     CDS             complement(1817..2857)
                     /gene="trpD"
                     /locus_tag="VFX94_13760"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX5769296.1"
                     /translation="MKQITIAEAIQRTVEHREVFHDEMLHVMRQIMRGELSPAQIAGF
                     IIGLRVKKETIGEIAAAAQVMRELATPVEVADERHLVDTCGTGGDAAHTFNVSTCAAF
                     VAAAAGAKVAKHMGRSVSSSSGSAEVLEAFGAHIGLGPGDTAQAIEHLGIGFMFAPAH
                     HAAMKYAAPVRKDLGVRTLFNILGPLTNPAGAKQQLMGVFHPDLVGIQVRVLQRLGSR
                     HVMVVYGLDGLDEISISGPTMVGELVKGDINEYNLHPGELGLELYDRRAIEVHTVAES
                     KAMIQAVLEGQPGPAQNIVALNAGAAIYVAGVANTLKAGVERAVQAIRSGAAKQKLEN
                     FVAFTQKVKAKA"
     gene            complement(2854..>2981)
                     /locus_tag="VFX94_13765"
     CDS             complement(2854..>2981)
                     /locus_tag="VFX94_13765"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898208.1"
                     /note="TrpG; with TrpE catalyzes the formation of
                     anthranilate and glutamate from chorismate and glutamine;
                     TrpG provides the glutamine amidotransferase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate/aminodeoxychorismate synthase
                     component II"
                     /protein_id="HEX5769297.1"
                     /translation="GEIMGLRHRSLPVEGVQFHPEALLTEHGHRMLENFLKGRTS"
BASE COUNT          512 a          979 c          943 g          547 t
ORIGIN      
        1 ttttgtaggt catctgctcg tatgcgaagc cgatgtccat aaagccgaag ttatacccgc
       61 ctgttacacg ccagccggta tcgtcattcc ccacgcttgt aaagtccatg tgcgtgtcga
      121 aagcagcacc cacgcgcagc ctgccgccca tgcccgccca ggtcaccgcg gcggaccaca
      181 tctgaggatc tgctgtgacg cccgtcgacg ctttgtcttg gttggtttgg taggcgacct
      241 tgacctgagc gccgccgaac accggcgact ggtagtgaac cgagttcgag atgcgacgcc
      301 agaaggggtg tcccacaccg ccgccacctt ccaccgaatc gcaggacgtt acgccgccga
      361 cgccggcgcc agtgttgccg cagttcgggt tcggcgcaga tcctgtcgtc tcgcggcgcc
      421 caatgatcga tgtgatcgga ccatacgtcg ctacggtacc gatgccccag agcgcatcga
      481 ggtccgccca cggcgtggtc cactggccaa cgaagaggtt accccagttg ccttgcaggc
      541 cgactgcgga gttccgggag gcgaagttcg acgtatgagc gacgttgttc gagcgctcca
      601 tcgatgcgtt cgtctctacc tgcgcccagg cgctaagacc accgccgaga ttctcgcgag
      661 cgcgcacgcc gatgttcgaa gcgtgcgagg cgacgtcgaa cttcgaaacc gacggaactc
      721 cgccggtgcc ctcaccgtac tgcatgtagt tcaaggacat gtggatggtg ccgtacacct
      781 ccacctgtgc cagcgcggca gcgggcgcgg ccaacgcccc ggctacagcc agcgatagaa
      841 gtttcttctg cattcgaatg ctcctctcct ttttggtttg aaaccggcgc ggcgccggac
      901 ccggcatgcg ccaacccctc tcctttgaga agtcgttgga gagtgtgccg aatgccccta
      961 tggcccaaca aggaatcagc ggtgttacac aggagaaggt aggcggatgt tgtgaatctc
     1021 caacatccct tgctgcattg cagcagctag aagaagagcg atctcaacgc aaatcccggg
     1081 tcggaggcgc gcatgaaggc ctcgccgacg aggaaggtat gcacgccggc atctcgcatg
     1141 cgcgctacgt ctgccggggc gcggatgccg ctctccgtga tgacgatgcg gtcgttcggc
     1201 acgcgcggga ggagctccaa ggtggtctcg aggcgggttt caaaggtgcg caggctgcgg
     1261 ttattgatgc cgacgagcgg cgtcttgagc gcgagcgcgc gctcgagctc gccggcatca
     1321 tgcacttcca cgagcaccgc catgccgagc tccagcgcca agtcttcgag ctcgcgcatc
     1381 tgcgcgtctc cgaggcaggc ggcgatcaag aggacgcagt cggcacccaa cgcccttgac
     1441 tcatgggcct gataaggatc gatgaggaag tccttgcgca gcgctgggag cgcacacgcg
     1501 gctcgcgcct gctgcagatg ggtggaagag ccttgaaaga actgcggatc ggtcagcacc
     1561 gacatgcagg cggcgccggc ctgctcgtag cttcgcgcaa tcgctgccgg atcgaaatct
     1621 tcgcgcaaca atcccttcga agggctggcg cgcttgatct cggcaatcac ggccgggcgc
     1681 ttggagcgca gggcgccgac gaagtcgcgc gccggcgtag ccgtcgccgc ccgcgcacgt
     1741 atttcttttt caggaatctt ttccttcgcc gcgacgactt cggcgcgctt tgccgcgagg
     1801 atgcgctcga gaatgctcac gccttcgctt tgaccttctg cgtgaaggcg acaaaattct
     1861 ccagcttctg cttggccgcg ccgcttctga tcgcttgcac ggcgcgctcg acgcccgctt
     1921 tcaaggtgtt cgccaccccg gcaacgtaga tcgcggcgcc ggcgttcagc gccacgatgt
     1981 tctgcgccgg acccggctgg ccttccagca ccgcctggat catcgccttg gattccgcca
     2041 cggtgtgcac ctcgatcgcc cggcgatcgt agagctccag gccaagctcg cccggatgaa
     2101 ggttgtactc gttgatgtcg cccttcacca gctcgccgac catggtcggg ccggagatcg
     2161 agatctcgtc gaggccgtcg agcccgtaga ccaccatcac gtgccgggag ccgaggcgtt
     2221 ggagtacgcg cacctggatg ccgaccaggt ccgggtggaa cacgcccatc agctgctgct
     2281 tggctccggc cgggttggtc agagggccga ggatattgaa gagggtgcgc acgcccaggt
     2341 ccttgcgcac cggggcggcg tacttcatcg cggcgtgatg cgcgggcgcg aacatgaacc
     2401 cgatgccgag atgctcgatc gcctgcgcgg tatccccggg gccgaggccg atatgtgcgc
     2461 cgaacgcctc gagcacttcg gcgctcccag acgacgatga caccgagcgc cccatgtgct
     2521 tggcgacctt cgcgccggcc gcggcggcga cgaaagcggc gcaggtcgaa acgttgaagg
     2581 tatgcgccgc gtcgccgccg gtcccgcacg tgtcgacgag gtgccgctcg tcggcgacct
     2641 cgaccggcgt cgccagctcg cgcatcacct gcgccgcggc ggcgatctcg ccgatggtct
     2701 ccttcttcac gcgcaggcca atgatgaagc cggcaatctg cgcggggctg agctcgccgc
     2761 gcatgatctg gcgcatgaca tgcagcatct cgtcgtggaa cacctcgcgg tgctcgacag
     2821 tacgctggat cgcctcggcg atcgtgatct gcttcatgag gtcctcccct tcagaaaatt
     2881 ctcgagcatt cggtgcccgt gctcggtgag cagggcctcg ggatggaatt gcacgccttc
     2941 gaccggcagg ctgcgatgcc gcagtcccat gatctcgcca t
//
LOCUS       DASUNT010000687         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_1139150, whole genome shotgun sequence.
ACCESSION   DASUNT010000687 DASUNT010000000
VERSION     DASUNT010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139150"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VFX94_13770"
     CDS             complement(<1..699)
                     /locus_tag="VFX94_13770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5769298.1"
                     /translation="MRNDATVIGLVSFAHGMSHFYQLLLAPLFPFIREDLGVSYAALG
                     FLVALFYTLSGLLQPLAGFVVDRYGARSVLLAGVACLVGSTLIMGFSTSYATLALGAA
                     LGGMGNSVFHPADFAILNARVSSARLGYAFSAHGVVGYVGYVLAPVVSVAIGAAFGWR
                     SALLAGAILGVGLLALLVFSSKHLEIEPQPKKKASIMTEASVLFAAPVVLCFAYFTVF
                     AAGLAGLQSFGVSAM"
     gene            complement(696..1463)
                     /locus_tag="VFX94_13775"
     CDS             complement(696..1463)
                     /locus_tag="VFX94_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5769299.1"
                     /translation="MPYVRAAGRSLAYEWIGEARDGGRTLLLLHEGLGSVRQWRDFPA
                     KLAAATGCRALVYDRYCYGQSDVLAEPRRTVRFMHDEALDALPQLMTSLKVEEPILVG
                     HSDGASIALIHAGAGHAVRGVVAMAPHVFIEPVCLSSIKEATATFESTDLAERLGRYH
                     RDARKTFYGWADVWLDPQFEGWDIRDDYLPQVRCPVLAIQGHDDEYGTMAQLDEIARR
                     VAGPCELLKLESCGHAPFRDQPDTVIRAVSAFLRKIL"
     gene            1500..>2006
                     /locus_tag="VFX94_13780"
     CDS             1500..>2006
                     /locus_tag="VFX94_13780"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5769300.1"
                     /translation="MRFTAWIVLFATLMLWSGNWIVARAVREEIAPGLATVGRLAIVL
                     GILAPFALDGLRHRLPVLARRDWRVLAALGFTGGGLHLALQWLGLHYTTATSGILYLS
                     MSPIFILVMAAPLGERIGPRQWAGVAISFCGVYLIATQGRPAVLSFNIGDLMALASMV
                     MWAGYTVLL"
BASE COUNT          359 a          662 c          681 g          304 t
ORIGIN      
        1 catcgcggac acaccgaagc tctgcagtcc cgcgagccct gcggcgaaca ccgtgaaata
       61 ggcgaagcag agcacgaccg gggcggcgaa aagcacgctc gcctcggtca tgatcgacgc
      121 cttcttcttc ggctgcggct cgatctcgag gtgcttggag ctgaaaacga gcagcgcgag
      181 gaggcctacg ccgaggatcg ctccggcgag aagcgccgag cgccagccga acgcggcgcc
      241 gatcgcgacg ctgaccaccg gggccaatac atagccgacg tagccgacca cgccgtgcgc
      301 gctgaaggcg tagcccaggc gtgcgctgct gacccgcgca ttgaggatcg cgaagtctgc
      361 gggatgaaac acgctgttgc ccatgccgcc aagcgcggcg ccgagcgcga gggtcgcata
      421 gcttgtcgaa aagcccatga tgagcgtcga tccgacaagg caggcgaccc cggccagcag
      481 aacgctgcgc gcgccgtagc ggtcgacgac gaacccggcg aggggctgca gcagccccga
      541 cagcgtgtag aagagcgcaa caaggaagcc gagcgccgcg taggaaacgc ccagatcctc
      601 ccggatgaaa gggaaaagcg gcgcgagcag gagctggtag aagtggctca tgccgtgcgc
      661 aaaagagacg agcccgatga cggtcgcgtc gttcctcaca ggattttccg gagaaaagcg
      721 ctgaccgcgc gaatcaccgt atcaggctgg tcgcggaacg gcgcatggcc gcagctctcc
      781 agcttgagga gctcgcaagg gccggctacg cggcgcgcga tttcgtcgag ctgcgccatc
      841 gtgccgtact cgtcgtcgtg gccctggatc gccagcacgg ggcagcgcac ctggggcaga
      901 taatcatcac ggatgtccca gccttcgaac tgcgggtcga gccacacgtc cgcccagccg
      961 tagaaagtct tgcgtgcatc gcggtggtag cggcccagcc tctcggcgag gtcggtgctc
     1021 tcgaaagtcg cggtggcctc cttgatcgaa gaaaggcaga ccggctcgat gaagacatgt
     1081 ggcgccatgg cgacgacgcc gcgcacggca tgcccggcgc cggcatggat cagtgcgatc
     1141 gacgcgccat cggaatgccc gaccaggatc ggctcttcaa ccttcaacga ggtcatgagc
     1201 tgcggcaaag catcgagggc ttcgtcgtgc atgaagcgga ccgtgcgccg cggctcggcc
     1261 agaacatcgg attgcccgta acaatagcgg tcgtacacga gcgcccggca accggtggcc
     1321 gcggccaact ttgccgggaa gtcgcgccat tgccggaccg agcccagacc ctcgtgcagc
     1381 aagagcagcg tgcggccgcc gtcgcgcgcc tcgccgatcc actcgtaagc gagcgatcgg
     1441 ccggccgcgc gcacgtatgg catcggtcga gcgtagcacg cagtggataa tcgcccgcca
     1501 tgcgtttcac cgcctggatc gtgctcttcg ccacgctcat gctttggtcg ggaaactgga
     1561 tcgtggcgcg cgcggtgcgc gaggagatcg cgccgggact cgccaccgtc gggcggctcg
     1621 caatcgtgct cgggatcctc gctcctttcg cgctcgacgg actgcggcac aggcttccgg
     1681 tgctcgcgcg ccgggactgg cgggtgctcg ccgcgctcgg attcaccgga ggcgggctgc
     1741 atctcgcgct gcagtggctc ggcctgcact acaccaccgc gacgagcggc atcctctacc
     1801 tctcgatgtc gccgatcttc atcctcgtga tggcggcgcc gctcggcgag cgtatcgggc
     1861 cgcggcaatg ggcgggcgtg gcgatttcct tctgcggcgt ctacctcatc gccacgcagg
     1921 gccggccggc agtgctctcg ttcaacatcg gcgacctcat ggcgctcgct tcgatggtga
     1981 tgtgggcggg ctacaccgtg ctgctg
//
LOCUS       DASUNT010000688         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_87599, whole genome shotgun sequence.
ACCESSION   DASUNT010000688 DASUNT010000000
VERSION     DASUNT010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87599"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFX94_13785"
     CDS             complement(<1..182)
                     /locus_tag="VFX94_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769301.1"
                     /translation="MSLLLGRADVERLLPAGVCIEAVEEAFRDHALGKVPPPGILGMH
                     AARGGFHVKAGFLGGY"
     gene            complement(179..1135)
                     /locus_tag="VFX94_13790"
     CDS             complement(179..1135)
                     /locus_tag="VFX94_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX5769302.1"
                     /translation="MKRYALKPLHCRPWLLQGLSPKLIESHYENNYGGALKRLNAIAA
                     ELDGADFARLPGFTLNGLKREELIAHNSVILHELYFASLGGDGRDPGSFAEVLTRDFG
                     SVDRWKAEFAAMGRALGGGSGWVLLTHVPRDGTLVNQYASDHSQSLASAIPILALDMY
                     EHAYHIDFGANAAAYVDTFLRNIDWKGVAARYHGALKVEPLADLVQKEFGDLPGVSVE
                     EVKTLLDSGQRVQFIDARPKHYVTRTQDIIDGATWRDPERVKEWAGELSKSDPVVVFC
                     VYGFHIGCQTAGTLREAGFDAKYMKGGHAAWKAIGGPVKRFP"
     gene            complement(1132..1746)
                     /locus_tag="VFX94_13795"
     CDS             complement(1132..1746)
                     /locus_tag="VFX94_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5769303.1"
                     /translation="MSRKVLWFLAAVSLFSTTAAAAEQTWVLGVDGVHLQQPGKARVA
                     IKLPEWHWAHEPYACPPAIAIGPRGEALVTSNVLPVLWKIDPQTLAVTVHSLQLDADS
                     DKDVGFSGLVYSARDNAYFAVSELHGTLWRIDPLLRRGQKIALSEPVRGACSISTQRE
                     QRVSRFTRLCLQGKSVNLAPDQRSAYVQSAGCASNPFALTGGSR"
     gene            1939..>2462
                     /locus_tag="VFX94_13800"
     CDS             1939..>2462
                     /locus_tag="VFX94_13800"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HEX5769304.1"
                     /translation="MFQTLVLSLPTRNSTLRMRVWRALKECGAAVLRDGVYLLPQAEA
                     FDKLEKEIRAHGGFAMTFDATPHGEDDQAALRARFDRTAEYAALVARFSAARASLPTL
                     GPRRGLTALRRLEQGLDKLARIDFFAGQAKEQAVAALASLKARYAGLYAHNEPRSSMK
                     GLRPIERARYQKRL"
BASE COUNT          412 a          842 c          798 g          410 t
ORIGIN      
        1 aagtagccgc cgagaaaccc ggcctttacg tggaagccgc ccctggccgc atgcatgccg
       61 aggattccgg gcggcggaac cttgccgagc gcgtggtccc ggaaggcctc ctccaccgcc
      121 tcgatacaga cgccggccgg cagcaggcgc tccacgtcgg cgcgaccgag cagcaagctc
      181 atgggaagcg cttcaccggc ccgccgatcg ccttccacgc cgcgtgcccg cctttcatgt
      241 atttcgcatc gaagccggcc tcgcgcagcg tccctgcggt ctggcatccg atatggaacc
      301 cgtagacgca gaaaaccacc accgggtcgc tcttcgacag ctcgccggcc cactccttta
      361 cgcgctcggg gtcgcgccac gtcgcgccgt cgatgatgtc ctgggtgcgc gtcacgtagt
      421 gcttgggccg cgcatcgatg aactggacgc gctgcccgga gtccagcagg gtcttcacct
      481 cttccacact cacgcccggt aaatcgccga actccttctg caccaggtcc gcaagcggct
      541 ccaccttcaa cgcgccgtgg tagcgcgccg ccacgccctt ccagtcgatg ttgcgcagga
      601 aggtatcgac gtaggcagcg gcgttcgcgc cgaagtcgat gtggtaagcg tgctcgtaca
      661 tgtccagcgc gaggatcgga attgcgctcg ccagcgactg gctgtgatcg gaggcgtact
      721 ggttaaccag cgttccgtcc cgcggcacat gcgtgagaag cacccagccg gagccgccgc
      781 ccagcgcccg gcccatcgcc gcgaattcgg ccttccagcg gtccaccgag ccgaagtcgc
      841 gggtcagcac ctcggcgaag cttcccggat cgcgcccgtc gcccccaaga ctggcgaaat
      901 acagctcgtg aaggatcacc gaattgtgcg cgatcagctc ctcgcgcttc agtccgttga
      961 gcgtgaagcc gggcagcctg gcgaaatcgg cgccgtcgag ctcggctgca atcgcgttca
     1021 ggcgcttcag agcgccgccg tagttgttct catagtggct ctcgatcagc ttcggcgaga
     1081 gcccctgcag cagccacgga cggcagtgca gcggtttcaa ggcatagcgc ttcatcgtga
     1141 acctccggtc agggcgaagg gattggacgc gcagccggcc gactggacgt aggccgagcg
     1201 ctggtcgggc gcgagattga cgctcttgcc ctgcaggcaa agcctggtga agcggctcac
     1261 gcgctgctcg cgctgcgtgg aaatcgagca agcgccgcgt accggctcgg aaagcgcgat
     1321 tttctgcccc cggcgcagga gcggatcgat gcgccacagc gtgccgtgga gctcgctgac
     1381 cgcaaaatag gcgttgtcac gcgccgagta caccagcccc gagaagccga catccttgtc
     1441 ggaatcggcg tcgagttgca ggctgtgcac ggtgaccgcg agggtctgag gatcgatctt
     1501 ccatagcacc ggcagcacgt tgctcgtcac cagggcttcg ccgcgcggcc cgatggcgat
     1561 cgccggcggg caggcgtagg gctcgtgtgc ccagtgccac tcgggcagct tgatcgcgac
     1621 ccgggccttg cccggctgct gcagatgcac accatcgaca ccgaggaccc atgtctgctc
     1681 ggcggctgct gcggtagtcg aaaacaatga aacggcggcg aggaaccaaa ggactttcct
     1741 gctcatgacg cactcctttc cctttcggga gctcggagca gcgcttgaga cgaggtgtct
     1801 tgactgccgg gtgtctgcct taggacccgg ccagggcgtc gccctgcatg agcggcacct
     1861 taaggagccc gccgccggcg gtcaacgccc ccggcgcact tagtggcaca tttctgtgat
     1921 acgtataaca tgcctgcgat gttccagacc ctggtcctca gcctgccgac gcgcaattcg
     1981 acgctgcgca tgcgcgtctg gcgcgcgctc aaggaatgcg gggcggccgt gctccgggac
     2041 ggcgtttacc tgctgccgca agcggaagcc ttcgacaaac tggagaagga aatccgggcg
     2101 cacggcggct ttgcgatgac gttcgatgca acgccgcatg gcgaggacga ccaggccgcc
     2161 ctgcgtgccc gcttcgaccg cacggcggag tatgccgccc tggtcgcccg cttttcggcc
     2221 gcgagagcct cgctgccgac gctcggcccc cggcgcggcc tgaccgcgct gcgtcgcctc
     2281 gagcaaggcc tggacaagct agcgcgcatc gactttttcg ccggccaggc gaaggagcag
     2341 gccgtcgcgg cgctcgcatc gctcaaggcg cgctatgccg ggctctatgc gcacaacgag
     2401 ccgcgctcgt ccatgaaggg tctgcggccg atcgagcgcg cgcgctatca gaagcgtttg
     2461 tg
//
LOCUS       DASUNT010000689         2562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_963546, whole genome shotgun sequence.
ACCESSION   DASUNT010000689 DASUNT010000000
VERSION     DASUNT010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963546"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /gene="ligA"
                     /locus_tag="VFX94_13805"
     CDS             complement(<1..708)
                     /gene="ligA"
                     /locus_tag="VFX94_13805"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF013790.4"
                     /note="this protein catalyzes the formation of
                     phosphodiester linkages between 5'-phosphoryl and
                     3'-hydroxyl groups in double-stranded DNA using NAD as a
                     coenzyme and as the energy source for the reaction;
                     essential for DNA replication and repair of damaged DNA;
                     similar to ligase LigB; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX5769305.1"
                     /translation="MNVEKRARKLRAAIEQHNRLYYVEDAPEITDAEYDALFRELQAL
                     EAEHPELRSADSPTRRVGGAPLAEFGEVRHRTPMLSISNAFEEEDVRAFDRRVCQALG
                     TERVQYATEPKLDGLAVSLVYRDGAFAQGATRGDGKTGEDVTANLRTVRSIPLATKAK
                     YLEVRGEVLMYRRDFDALNKRQREAGQKEYVNPRNAAAGAVRQLDSRITASRPLRFFA
                     YAIAAAERIDWQTQAQAL"
     gene            complement(705..1559)
                     /locus_tag="VFX94_13810"
     CDS             complement(705..1559)
                     /locus_tag="VFX94_13810"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HEX5769306.1"
                     /translation="MSDLVLGLLVLGAVLFAAVLVYNRLQERSARRAIERAFDSRAED
                     MLAHAPPERREPTLEKGPAQPVPDTRVDYVIELKPTRALSAAAVQEGWLQIDQRFGRR
                     AFLANSDGVWHAALQLVSRAGAVSEGELIEFRSAVETLAARLGASAAAPETRPALEVA
                     RELDRACADGDIQVALHVVGDGVTPEAIDGDFGEQPFQVVRRENGVTLVLDVALTRDL
                     GASYEAMARAGRSLAAAHNARLVDDRGNPLDERALAAIGAQLETVRQTLAARGIETGS
                     ALALRLFS"
     gene            complement(1594..>2562)
                     /locus_tag="VFX94_13815"
     CDS             complement(1594..>2562)
                     /locus_tag="VFX94_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX5769307.1"
                     /translation="PIPSVREALENAVELRIACEKQLAEGRNAVEAAAGALRELEEAK
                     LQAETRAAPLREKLGELRLKEQAARLNFDQFATQLSEAGANEEALAAEAERAPRASSL
                     QGEITRITQALNELGAVNLAALGELSASRERKSFLDAQATDLEEAVKTLEDAIRKIDR
                     ESRELLRETFDTVNGHLAKLFPTLFGGGDAKLVMTGEEILDAGVQVMAHPPGKRNTSI
                     HLLSGGEKALTAIALVFSLFQLNPAPFCLLDEVDAPLDDSNTLRFCDLVKRMSAQTQF
                     LFISHNKISMEMASQLVGVTMPESGVSRVVAVDIEEALRIREELAA"
BASE COUNT          377 a          900 c          849 g          436 t
ORIGIN      
        1 gagcgcctgc gcctgggtct gccagtcgat gcgctcggca gccgcgatcg cgtaggcgaa
       61 aaagcgcagc ggccgcgacg cggtgatgcg ggaatccagc tggcgcaccg ccccggccgc
      121 tgcgttgcgc ggattgacgt actctttctg cccggcctcg cgctgacgct tgttcagcgc
      181 gtcgaaatcg cgccggtaca tcagcacctc gccgcggact tcgaggtatt ttgccttagt
      241 tgctagggga atggagcgaa ccgtacggag gttcgccgta acgtcctctc cggttttgcc
      301 gtcgccgcgc gtggcgccct gggcgaaggc gccatcgcgg taaaccaggc tcaccgcgag
      361 gccgtcgagc ttaggctcgg tcgcgtactg gacccgctcg gtgccaagcg cctggcacac
      421 ccgccggtcg aaggcgcgca cgtcctcttc ctcgaacgcg ttgctgatcg aaagcatcgg
      481 cgtgcgatgc cgcacctcgc cgaattcggc gagcggcgcg ccgcctacgc gccgggtcgg
      541 cgaatctgcg ctgcgcagtt ccggatgctc ggcttccagc gcctgcagct cgcgaaacag
      601 cgcatcgtac tcggcgtcgg tgatctcggg cgcgtcttcg acgtagtaca gccggttgtg
      661 ctgctcgatc gctgcgcgaa gttttcgtgc tcttttttcg acattcatga aaacagacgc
      721 agtgccagcg cgcttccggt ctcgatgccg cgcgctgcca gggtctggcg caccgtctcg
      781 agttgcgcgc cgatcgcggc gagcgcgcgc tcgtcgagcg gattgccgcg atcgtcgacg
      841 agccgcgcgt tgtgcgccgc ggccaggctg cgtccggcgc gcgccattgc ctcgtaactc
      901 gcaccgaggt cccgcgtaag cgccacgtcg aggacgagcg tgacgccgtt ctcgcgccgc
      961 accacctgga acggttgctc gccgaagtcg ccgtcgatcg cctccggggt cacgccgtcg
     1021 cccacgacat gcagcgccac ctggatgtcg ccgtccgcgc acgcgcggtc cagctcgcgc
     1081 gccacctcca gcgcgggccg tgtctccggc gccgccgcgc tcgcgccaag ccgtgcggcc
     1141 agggtttcga ctgcggagcg gaactcgatc aattctcctt cgctcaccgc gccggcgcgg
     1201 ctgacaagct ggagcgcggc gtgccagacg ccgtcggagt tggcaaggaa ggcgcggcgg
     1261 ccgaaacgct ggtcgatctg cagccagccc tcctgcaccg cggccgccga gagcgcccgc
     1321 gtgggcttga gctcgatcac gtagtcgacc cgggtatccg gcacgggttg ggcaggaccc
     1381 ttttccaggg tgggctcccg ccgctccggc ggcgcgtgcg ccagcatgtc ctcggcacgc
     1441 gagtcaaacg cgcgctcgat cgcgcgccgc gccgagcgct cctgcaggcg gttatagacc
     1501 agcactgcgg caaagagaac cgcgccaagc acgagcagac cgagtacgag gtcgctcatg
     1561 acacgcgaga cggggaaaca ggccgcccca gaatcaggcg gccagctcct cccggatgcg
     1621 cagcgcttcc tcgatgtcga ccgccacgac gcgcgacacg cctgactcgg gcatggtgac
     1681 gccgacgagc tgcgaggcca tttccatcga gatcttgttg tggctgatga acaggaactg
     1741 cgtctgcgcc gacatgcgct tcaccaggtc gcagaagcgc agggtattcg agtcgtcgag
     1801 cggcgcatcg acctcgtcga gcaggcagaa cggcgcggga ttcagctgga acaacgagaa
     1861 cacgagggcg atcgcggtga gcgctttctc gccgcccgag agcagatgga tgctggtatt
     1921 gcgcttgcct ggaggatgcg ccatcacctg cacgccggcg tcgaggatct cctcgcccgt
     1981 catcacaagc ttcgcatcgc cgccgccgaa aagcgtgggg aagagcttcg ctaggtgccc
     2041 gttcacggta tcgaaggtct cgcgcagcag ctcgcgactc tcgcggtcga tcttgcggat
     2101 tgcgtcttcg agcgtcttca ccgcctcttc gaggtcggtg gcctgcgcgt cgagaaagct
     2161 cttgcgctcg cggctcgcgc tgagctcccc gagcgcggcc aagttcaccg cgccgagctc
     2221 gttgagcgcc tgggtgatgc gcgtgatctc tccctgcagc gaagacgcac gcggcgcgcg
     2281 ctcggcctcc gcggcgagag cctcctcgtt cgcccccgcc tcgctgagct gcgtggcgaa
     2341 ctggtcgaaa ttgagccgag ccgcctgctc cttcaggcgc agctcgccga gcttttcgcg
     2401 cagcggggcg gcacgcgttt ccgcctgcag cttcgcctct tccagctcgc gcagcgcgcc
     2461 ggcggccgct tccaccgcgt tgcgcccttc ggcgagctgc ttctcgcacg caatcctcag
     2521 ttccactgca ttttccagcg cttcgcgcac cgaagggatc gg
//
LOCUS       DASUNT010000690         4642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U1608
            k141_613512, whole genome shotgun sequence.
ACCESSION   DASUNT010000690 DASUNT010000000
VERSION     DASUNT010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961746
            Sequence Read Archive: SRR25158521
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,768
            CDSs (total)                      :: 2,755
            Genes (coding)                    :: 2,722
            CDSs (with protein)               :: 2,722
            Genes (RNA)                       :: 13
            tRNAs                             :: 10
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 27 of 33
            Pseudo Genes (internal stop)      :: 3 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613512"
                     /isolate="SMAG_U1608"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.51166667 N 112.3322222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VFX94_13820"
     CDS             <1..524
                     /locus_tag="VFX94_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methanesulfonate monooxygenase"
                     /protein_id="HEX5769308.1"
                     /translation="LVKRKPDGAFSEWLFGANREGARLETFGPLGKATFEPGLGKNLL
                     LVAGGSGIAGMMSILARAEAANYFAHHKGWVFFGVRTMKDAFFLEELSQTKAEVTVAL
                     SEDDVPQHNAWPGLKFQKGLVHEVAKSAMAGKYHNVRAYVAGPPPAVDAAIRVLLLDA
                     KLTTDNIRYDKFS"
     gene            524..1255
                     /locus_tag="VFX94_13825"
     CDS             524..1255
                     /locus_tag="VFX94_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5769309.1"
                     /translation="MAKRMVCRVADVPENSLKECEAEGGLKLVVANAGGEFVGFQAMC
                     PHQDVPLCEGLFDGATLTCHMHLWQWDVRSGAPLGIAEAPLQRYPLAREGDALYLGGE
                     STALDVGELFNGLNSSILDKLATLAKRENHPSGAVLYRPGDLADDFYVLDSGRIEFLI
                     GRGDRAAPGGFMLRKGEVFGWAALLEGTPARIASARCLEDSSVLRMNGKRALAVLEGD
                     AAAGFVVMRRLAALIARYLASSGAR"
     gene            1281..2384
                     /locus_tag="VFX94_13830"
     CDS             1281..2384
                     /locus_tag="VFX94_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5769310.1"
                     /translation="MKRRHVIAAAIAASLSGAALAQDYGKPGGPVTVVVGYQPYYTQS
                     WSGVVMRGKKFYEKYLPKGSNVEFQIGLQGAIIVNNMLAGKQHIGYMGDMPSIVATTK
                     TEVADVRLVANLGLAYDQCNAFLVRNDAPQFKNDQEAIRWLAGKTVAVPKGSCTDRFA
                     QATFKRFNIQPKEYLNQNIEVITSNFRAGKLDAAVMWQPTTSRLVDEGLARLVATGKS
                     VQENDGGFLAMRADLIKQRPDVVKAWLQAELDAQLFIADAKNAPEVIKMAKEQTTGFS
                     EKVLWNSLYGRYPDAAGGGEMRNTMHYTFTPEARELITRAATFLHSIKSINVEKVRPE
                     AIEAQWTEEILKERGLKAPIAEVKALPASQAPK"
     gene            2388..3731
                     /locus_tag="VFX94_13835"
     CDS             2388..3731
                     /locus_tag="VFX94_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5769311.1"
                     /translation="MEVAATANGAVDEAAAALRASTPFRDVPERLLARVVALARPARY
                     AAGTRIYHAGDPADDVFVVVSGRVDHVFKPEVGAHEPLKRMTRGGVFGWAGLLLGQTR
                     RLATVTATEPAEVLRIDTEALIAALESEPLEGARVMERFVGMIQREFTVPAMLAQVRR
                     LSGPLTEEMSSLSLTMYRVSLWLRSPRPYLMLVGFALFLGFWYLAVEVWRLPRFREMP
                     GVTAVVKEWLSPNPTYGLSLYTEEYYQHIIVSVWRVAKAFSLATVLGVTFGLLLGWSR
                     AFKDYVFPVFEMLRPIPILAWVPLAIVMFIATESAVVYLAFLASFFATALNTMLGVES
                     IDESYIRAANCLGASRWQVFRHVIIPGALPFIFTGLQISIGVSWFSLVAAEMVSGQYG
                     LGYVINTSYVMVRYPTIVIGMVTLGIVGYVTSAMVRLAGDYLMEWRVRELALAER"
     gene            3728..4549
                     /locus_tag="VFX94_13840"
     CDS             3728..4549
                     /locus_tag="VFX94_13840"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5769312.1"
                     /translation="MSRGQISIQDVVKVYDPQGAAVMAVDHCSLDIEAGEICMIVGPS
                     GCGKTTLLNAIAGFHEITAGRIVMDGEILCSSERPKSTPGPDRIVVFQNGALFPWKSN
                     LENVIFGPVMQGKLSRRDALDKGRAMMSDAGLSGVEHNYPGEVSSGMRRRVEIVRALM
                     NEPKALLLDEPYRALDSLTKSVMHEALLEIYYKNRVTIFFITHDLEEAIFLGHRVAIM
                     TTRPCRPKKILEVDIPHPRNYSVLTSPRFRELMEETVSAVHEEAVKAFEAGEKEM"
BASE COUNT          794 a         1499 c         1593 g          756 t
ORIGIN      
        1 tcctggtgaa gagaaagccc gacggcgcat tctccgagtg gctgttcggc gcgaaccgcg
       61 agggcgcacg gctcgagacg ttcggcccgc tcggcaaggc gacgttcgag cccgggctgg
      121 gcaagaacct gctcctcgtc gccggaggct cgggcatcgc cggcatgatg tcgatcctgg
      181 cgcgggccga ggccgcgaac tacttcgcgc atcacaaagg ctgggtgttc tttggcgtgc
      241 gcaccatgaa agacgccttt tttctggagg agctctcgca gacgaaggcc gaggtaacgg
      301 tcgcgctttc cgaggacgat gtgccgcagc acaacgcgtg gcccggcctc aagttccaga
      361 agggcctggt gcacgaggtg gcgaagagcg cgatggccgg caaataccac aatgtgcgcg
      421 cctacgtcgc cggcccgccg ccggccgtgg acgcggcgat ccgggtcctg ctgctcgatg
      481 ccaagctcac caccgacaac atccgctacg acaaattcag ctgatggcga agcgcatggt
      541 ctgccgcgtg gccgacgtcc cggaaaacag cctcaaggaa tgcgaggccg agggcgggct
      601 caagctcgtg gtcgcgaacg ccgggggcga gttcgtcggc ttccaggcca tgtgcccgca
      661 ccaggacgtg ccgctgtgcg aggggctgtt cgacggcgcg acgctcacct gccacatgca
      721 tctgtggcag tgggacgtgc gcagcggcgc gccgctcggc atcgcggaag cgccgctgca
      781 gcgctacccg cttgcgcgcg agggcgatgc gctctacctc ggcggcgagt ccaccgccct
      841 cgacgtgggt gagcttttca atggattgaa ttcatcgatt ctcgacaagc tcgcaacgct
      901 ggcaaagcga gaaaaccacc cctcgggcgc ggtcctgtac cgccccggcg acctggccga
      961 cgatttctat gtgctcgatt ccgggcgcat cgagttcctg atcgggcgcg gcgaccgcgc
     1021 cgcgccgggc ggcttcatgc tgaggaaagg cgaggtgttc ggctgggcgg cgctgctcga
     1081 aggcacgccc gcgcgcatcg cgagcgcgcg ctgcctggag gattcgtcgg tgctgcgcat
     1141 gaacggcaag cgtgccctcg ccgtgctgga gggcgacgcg gcggcgggat ttgtggtcat
     1201 gcgccggctg gcggcgctga tcgcgcggta tcttgcatca tccggagcaa ggtgaagaca
     1261 accgaggaga ggagggcatc atgaagcgca ggcatgtcat tgcagcggca atcgcggcgt
     1321 cgctaagcgg cgcggcgctc gcgcaggact acggcaagcc gggcggaccg gtgacggtcg
     1381 tggtcggcta ccagccgtac tacacgcagt cgtggtccgg ggtggtgatg cgcggcaaga
     1441 agttctacga gaagtacctg ccgaagggct cgaacgtcga gttccagatc ggcctgcagg
     1501 gcgcgatcat cgtcaacaac atgctcgccg gcaagcagca catcggctac atgggcgaca
     1561 tgccgtcgat cgtcgcgacc acgaagaccg aggtcgcgga cgtgcgcctg gtcgccaacc
     1621 tgggcctcgc ctacgatcaa tgcaacgcct tcctcgtgcg caacgacgcg ccgcagttca
     1681 agaacgacca ggaagcgatc cgctggctgg cgggcaagac cgtcgcggtg ccgaagggaa
     1741 gctgcaccga ccgcttcgcg caggcgacgt tcaagcgctt caacatccag cccaaggagt
     1801 acctgaacca gaacatcgag gtgatcacct cgaacttccg ggcgggcaag ctcgacgcgg
     1861 cggtgatgtg gcagccgacg acctcgcgcc tggtggacga gggcctggcg cggctggtcg
     1921 caacgggcaa gagcgtgcag gagaacgacg gcggcttcct cgccatgcgt gccgacctca
     1981 tcaagcagcg tcccgatgtg gtgaaggcct ggctgcaggc ggagctcgac gcgcagctct
     2041 tcatcgccga cgcgaagaac gcgcccgagg tcatcaagat ggcgaaggag cagaccaccg
     2101 ggttctcgga gaaagtgctg tggaactcgc tctacggccg ctatcccgac gccgccggcg
     2161 gcggcgagat gcgaaacacg atgcactaca ccttcacgcc cgaggcgcgc gagctcatca
     2221 cgcgcgcagc cacgttcctg cactcgatca agagcatcaa cgtcgagaag gtgaggcccg
     2281 aggcgatcga ggcgcagtgg accgaggaga tcctgaaaga gcgcggcctt aaggcgccga
     2341 tcgccgaggt gaaggcccta ccggcttcgc aggcgccgaa gtaggcgatg gaggtcgccg
     2401 ccaccgcgaa cggcgcggtc gacgaagcgg cggccgcact gcgcgcaagc acgccgttcc
     2461 gtgacgtccc ggagcggctt cttgcgcgcg ttgtggcgct tgcccgtccc gcgcgctacg
     2521 ccgcgggcac ccggatttac cacgccggag acccggctga cgatgttttc gtcgtggtgt
     2581 cgggtcgagt cgatcatgta ttcaagcccg aggtgggcgc gcacgagccg ctgaagcgca
     2641 tgacgcgcgg cggggtgttc ggctgggccg gcctgctgct cgggcagacc cggcgcctcg
     2701 ccaccgtgac ggcgaccgag ccggccgagg tgctgcgcat cgacaccgag gcgctgatcg
     2761 cggcgctcga gtccgagccg ctcgagggtg cccgcgtcat ggagcgcttc gtcggcatga
     2821 tccagcgcga gttcaccgtg ccggcgatgc tcgcgcaggt gcggcggctc tccgggccgc
     2881 tcaccgagga gatgtcgagc ctcagcctca cgatgtaccg cgtgtcgctg tggctgagga
     2941 gcccgcggcc gtacctcatg ctggtcggct tcgcgctctt tctcggcttc tggtacctgg
     3001 cggtggaggt gtggcgcctg ccgcgcttcc gcgagatgcc cggcgtaacc gcggtggtca
     3061 aggagtggct ctcgcccaac ccgacctacg ggctttccct ctacaccgag gagtattacc
     3121 agcacatcat cgtatcggtc tggcgcgtcg ccaaggcgtt ctccctcgcc acggtgctcg
     3181 gcgtgacgtt cggcctcctg ctcggctggt ccagggcatt caaggactac gtcttcccgg
     3241 tattcgagat gctgcgcccg atcccgatcc tcgcctgggt gccgctcgcc atcgtcatgt
     3301 tcatcgccac cgaatcggcg gtggtgtacc tcgccttcct cgcctcgttc ttcgccaccg
     3361 cgctcaacac catgctcggc gtcgaatcga tcgacgagtc ctacatccgc gcggcgaact
     3421 gcctcggcgc gagccgctgg caggtgttcc gccacgtcat cattcccggc gcgctgccgt
     3481 tcatcttcac cggcctgcag atctcgatcg gcgtgtcgtg gttttcgctg gtggcggccg
     3541 agatggtgtc gggccagtac ggcctcggct acgtcatcaa tacgtcctac gtcatggtgc
     3601 gctatccgac gatcgtgatt ggcatggtga cgctcggtat cgtcggctac gtcacgagcg
     3661 cgatggtgcg cctcgccggc gactacctga tggaatggcg cgtacgcgag ctggcgctgg
     3721 cggagagatg agccgcggac agatctcgat ccaggacgtc gtcaaggtct acgacccgca
     3781 gggggcggcg gtgatggcgg tcgaccactg ctcgctcgac atcgaagcgg gcgagatctg
     3841 catgatcgtg ggcccctcgg gctgcggcaa gacgacgctg ctcaacgcca tcgccgggtt
     3901 ccacgagatc actgcggggc gcatcgtcat ggacggcgag atcctgtgct cgagcgaaag
     3961 gccgaaatcc acgcccgggc ccgaccggat cgtggtgttc cagaacgggg cgctgtttcc
     4021 gtggaagtcg aacctcgaga acgtgatctt cggcccggtg atgcagggca agctctcgcg
     4081 ccgggacgcg ctcgacaagg gccgcgccat gatgtccgac gccgggttgt cgggcgtcga
     4141 gcacaactat cccggcgagg tgtcctcggg catgcgccgg cgcgtggaaa tcgtgcgcgc
     4201 gctcatgaac gagccgaaag cgctgctgct cgacgagccg taccgcgcgc tcgactcgct
     4261 caccaagagc gtgatgcacg aagcgctgct cgagatttac tacaagaaca gggtgacgat
     4321 cttcttcatc acccacgacc tcgaggaggc gatcttcctc ggccaccgcg tggcgatcat
     4381 gaccacgcgg ccgtgccggc cgaagaagat cctcgaggtc gacatcccgc atccgcgcaa
     4441 ctacagcgtc ctcaccagcc cgcgcttccg cgagctgatg gaggagacgg tgagcgcggt
     4501 gcacgaggag gcggtgaagg cgttcgaagc cggcgaaaag gaaatgtaga gatgccttac
     4561 gtgaggggat gtcaggaatg agcgcagtcg caagcgccgc cgtgcgcccg ccggtcaaga
     4621 agccgggcaa gtcgtggttc tc
//
